piezo1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	piezo1
Entrez Gene ID	100488940
Full Name	piezo-type mechanosensitive ion channel component 1
Synonyms	fam38a
General protein information	Preferred Names piezo-type mechanosensitive ion channel component 1 Names piezo-type mechanosensitive ion channel component 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002933675.5	XP_002933721.3	piezo-type mechanosensitive ion channel component 1 isoform X1
XM_002933675.4	XP_002933721.2	piezo-type mechanosensitive ion channel component 1 isoform X1
XM_012962282.2	XP_012817736.1	piezo-type mechanosensitive ion channel component 1 isoform X3
XM_012962280.1	XP_012817734.1	piezo-type mechanosensitive ion channel component 1 isoform X2
XM_012962280.2	XP_012817734.2	piezo-type mechanosensitive ion channel component 1 isoform X2
XM_012962282.3	XP_012817736.2	piezo-type mechanosensitive ion channel component 1 isoform X3

Pathways from BioSystems

Homologies

	FAM38A	XP_001099984.2
Bos taurus (cattle)	PIEZO1	XP_002694905.2
Gallus gallus (chicken)	FAM38A	XP_414209.4
Homo sapiens (human)	PIEZO1	NP_001136336.2
Rattus norvegicus (Norway rat)	Piezo1	NP_001070668.2
Mus musculus (house mouse)	Piezo1	NP_001032375.1
Xenopus tropicalis (tropical clawed frog)	piezo1	XP_002933721.2
Danio rerio (zebrafish)	piezo1	XP_696355.4
Pan troglodytes (chimpanzee)	PIEZO1	XP_003952965.1
Canis lupus familiaris (dog)	PIEZO1	XP_005620632.1

Gene Ontology

The following piezo1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the piezo1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa56850	XM_002933675.5 Latest version!	Xenopus tropicalis piezo-type mechanosensitive ion channel component 1 (piezo1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXa56851	XM_012962280.2 Latest version!	Xenopus tropicalis piezo-type mechanosensitive ion channel component 1 (piezo1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXa56852	XM_012962282.3 Latest version!	Xenopus tropicalis piezo-type mechanosensitive ion channel component 1 (piezo1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXa08835	XM_002933675.4 Latest version!	Xenopus tropicalis piezo-type mechanosensitive ion channel component 1 (piezo1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OXa34346	XM_012962280.1 Latest version!	Xenopus tropicalis piezo-type mechanosensitive ion channel component 1 (piezo1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OXa34347	XM_012962282.2 Latest version!	Xenopus tropicalis piezo-type mechanosensitive ion channel component 1 (piezo1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

piezo1 ( XM_002933675.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002933721.3 Xenopus tropicalis piezo-type mechanosensitive ion channel component 1 (piezo1), transcript variant X1, mRNA.

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CloneID	OXa56850
Clone ID Related Accession (Same CDS sequence)	XM_002933675.5
Accession Version	XM_002933675.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7425bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	piezo-type mechanosensitive ion channel component 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002933675.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACTGC AAGTCCTCTG TGGGGTCCTG TTTTGGCTCC TTCTGCCCGG CTCCCTCTTG 
GCAGCCTGCT TATTCCGTTA CAACTCCTTG TCACTGGTGT ACCTGTTTTA TCTTCTGCTT 
CTGCCATGGT TTGCTGGACC AAATCCCTGC AGCATGAGAG GTCACACAGG ACGTCTGATG 
AAAGCGTTAC TTGCTACCAG CATCCTCTTC CTCTGTGCTC ATCTCATCTT CCAGATCTGC 
CTGTACACAA TACCTGAACT GGATGACCTG CTAGGGCACA ACTGCAGCAG CTGGGATGTG 
TTGGCTCGTC ACGTAGGTAT AAACAGGCTG GATCTTTCTG ATATCCCCAA CATGGTGCGA 
CAAGTGTCCC CAGATTTTGG CATGTTTGTC GTGTCAGCAT TGTGCCTTGG CTTGTGTAAT 
CGCCTAGTGA CCAAGAAGAG ACGACAGAGG TCTTCAGGCT ATTTTGAGCA GGAAGAAGAG 
AGGACTGAAC TTAACCTAAA ATATGAAAGT AGAGAGTGTG ATACAGAGTT TGAGGTGGGG 
GGAGACTTAC ATTCTGTTCC ACGAGACCGC CTTCTTGGGT CCTGGCTTAG AAAGGCAGCA 
TACAGGCTTC TGGAAACAGT GGAGAAATCA CTTGCTGTCT TACTCTTGGC TCTTGCAGGT 
ATCACTGATC CCTCTGCTTT CTCAGCCCCA TATTATGTGG TTTTCATTGG TCTCTGCACA 
TGGTGGGCCT GCCATTTTCC CATTAGCCAA CTAGGCTTTA ATACTATCTG TGTCATTGTT 
GGGTTCTTCA CTGGAGGTCA CATGATTTGC CTTTATATTT ACCAGACACC TTTTATCCAA 
CAAATGTTTC ATCCTGAACA GTTATGGGCA AGGTTGTTTG GTCTGCATAA CATCATCAGT 
CACTCCAACA TTACAGACTG TACCCATGAC TCTCTGAATC AAAACCTTGA CTGGCCTGTG 
TATGTTCATC CCGGAATCTT ATTACTGCTG TACTACTCCC TATTATCAAT ACTGAAACTG 
AAGAGCTCTG ACAAGAAAAG GATTTCAGGG TGTGACCTTC GGAAATCTCA GCAGGAGGAT 
ATGGAGTTGA CTGATATTGA GCCAGTTTCA AAGGAGCAAT ATAGTGCGGG AGAAGAGACA 
AAACCAATGC TTCAGAGGAT GAACACTGAA GAAGAGGCTA AACCCCAAGA CTGCACTGTT 
CATGTGATTA GAAGAAATTC TAATCAACTG CCAGAAGAAC TTGAAACCAA GGAGCCATCA 
CAATTTCACC TGATGATTCA TGTAATACTG AGCCAAAGCT ATATCTGTGC GCTTATTGCA 
ATGATGGTGT GGAGCATTAC ATATCACAGC TGGCTGACTT TTGTGCTGCT GCTCTGGGCA 
TGTTTAATCT GGATGGTGAG GAACAGGCGC CAGTTTGCAA TGCTGTGCTC TCCCTTTATT 
TTGATGTATG GAGTGACTCT CTGCAGCCTG CAGTATGTCT GGAGTATGGA CTTGAGAGAA 
GAGCTTCCGA CACAACTTGG TTTTATGAAG CTGTCACAGC TGGGACTGGT TCGCGCACCA 
TACCCCTGCC TTAACATGGG AGCACTGCTA CTTTACACGC TGACATTTTG GCTTCTGCTG 
CACCAATTTG TAACAGAAAA ATTTCTAAAA CGTAAGAAGT TTATGTCCCC ACTCCTGGAG 
GTCACAGTGG CAGATGCTGG CAAAGAAAAG AACCTGCTAA AACGTTTTGG GGAGCAAGTA 
AAAAGCTTCT ATGCAAAATA CTGGATCTAT GTGTGTGGAG GCATGTTTAT TGTGGTCAGC 
TTTGCTGGTA GGCTGGTAGT TTACAAAATA GTCTACATGC TTCTGTTCCT ACTATGCTTG 
GTGTTATTTC AGGTGTATTA CTCTTTGTGG AGGAAGCTGC TGACTTTTTT CTGGTGGTTA 
GTTGTAGCAT ACACAATGTT GGTTCTTATC ACCATCTACA CCTTTCAATT TGAAGAGTTT 
CCTGAGTACT GGAAAAACTT GACCAACCTT ACAGATGAGC AGTTGGGTGA TATTGGGCTT 
GAGAGGTTTA GTGTGTCGGA ACTTTTTACC AGCATCCTTA TCCCAGGCTT CTTCCTCCTT 
GCCTGCATCC TCCAGTTACA CTACTTTCAT CGCCCTTTTA TGCACATGAC CAACTTGGCA 
CATGCCTCAT GCCAAGGAAG TTTCCATAGC CACGAGAGTG AGCAGCCAAA GGAAGAAGAA 
GAGAAAGCTG GTGCATGCAG TGATGCTGCT GCTGATGAGG AGGAGGATGA GGAAGAATCT 
GCACATGGAG CTGATCTAAG TAAATGGGAC CTAGTTACAC AGCGCCTTTC TGTCCTGTCC 
TTTAAGTTCT CTGAGCTGCT GCACCGATTA CAGGTTTTCC TTTGGAGGCT TTTGGAGCTC 
CATGTGTTGA AACTGGTGGC TTTGTATAGC GTGTGGGTTG CCCTGCAGGA GGTATCTGTG 
ATGAATTTCC TTTTTGTGGT CCTATGGGCT TTTGCTATGC CTTTCTCCCG ATTCCAGCAC 
ATGGCTTCCT GTCTTTGCAC CATCTGGACC TCTATAATTA TTGTTTGCAA AATGCTTTAC 
CAGCTTACTG TTGTCAACCC TCTGGAGTAC TCTAGCAACT GTACTCAGCC TCCTCTAAAT 
AACACCAGTC TCCTAGAAGA CGAGATTTTG AACTCCACCC TTTATAGAGA ACCTATAGAT 
CCAGCAAACT GGTTTGGTAT CAGAAAAGGT TATCCAAACC TAGGTTACAT ACAGAATCAC 
CTGCAAATAC TCCTACTTTT GGTATTTGAG GCAATTGTTT ATCGGCATCA GGAGTTTTAC 
CGTAAACAAT ACCAGCTTGA GAAGCCACAC ATTCAGGCTG TATTTCCAGA TGTCTACAGA 
GAGCACTTGG ACCAAGGCTT GTTCAGCTGT ATCAAATATT TTATCAACTA TTCCTTTTAT 
AAGTTTGGTC TTGAGATCTG CTTCCTTCTT ACTGTGAATG TGATTGGTCA GAGGATGAAC 
TTTATGGTAA TCTTTCATGG CTGCTGGCTG ATAGTGATTC TTACTCGACG ACAGCGAGTC 
AACATTGCAA AATTATGGCC TAAATACTGT GTGTTCCTAA TCTTTTTCCT GTTGTGGCAG 
TACATGATAT GTCTGGGCAT TCCTCCGGTG CTTTGTATAG ATTATCCCTG GAGGTGGGAC 
ACATTTGTTC CTGTCAACTC TGCACTGATA AAGTGGTTAT ATCTTCCAGA TTTCTATACT 
ACACCTAAAT CTGTCAACCT TATCAGTGAT TTTGTTTTGC TGATTGGTGT GGCCCAGCAG 
TGGAAGGTGT TTATTAATGA GAAGAAGGAA GAATGGTACA CTATGGCAGG GGAAAATGTG 
GAAGAGTATG AAGCTCCAAA AGACAGGCCC AACCCTGTAC CTAACTTCAT CAATTGTCAG 
TCTTATCTGG ATATGGTGAA AGTAGCAGTG TTTCGGTACC TATTCTGGTT TGTCTTGACT 
GTGGTCTTTA TAACAGGGGC CACTCGCATC AGTGTTTTTG GCCTAGGATA TTTGATGTCC 
TGTTTCTACC TTCTTCTGTC TGGCACAACC CTTCTGCAAA AGCCAATGAA AGCCAAGCTG 
GTGCTTTGGG ACTGTTTGAT CCTCTACAAC ATCACTGTTA TACTGTCCAA GAACATGCTG 
TCGCTCCTGT CTTGTGTATT TGTTCAACAA ATGCAAACCA ACTTTTGCTG GGTGATCCAG 
CTCTTCAGCC TAGTTTGCAC AGTTAAGGGT TACTACAATC CCCAAGATAT GGATAAAGAC 
CGGGATTGCT CTCTGCCAGT GGAAGAGGCC GGAATCATTT GGGATAGCAT CTGCTTCTTA 
TTTCTCCTTC TCCAACGAAG AGTTTTTTTG AGTTGCTACT TTCGGCATGT TGTTCTTGAC 
CTTCGTGCCT CCTCCCTTCA AACATCAAGA GGTTTTGAGC TCTTCATGGC CAGCATCAGA 
AAGAATGTGT TTTTCCACCA AGAATCTGAG AGACGATCTC TTAACCAGCT CAGGAAGCAA 
ATGGAGAGAA TCCGAGCAAA GCAAGAACGC TATCATGAAG GCAGAAGACA AACTGATAGC 
AGTCAAGTAA AGGAAGGTGA GCCTGGCAGC AAGAAAGGGT CCAGTAGGCA AAAGATTCAT 
TGGAGCCGCC CATGGTTAGA CCACGCCTCA GTGCTGCATT CAGGAGATTA TTTTCTGTTC 
GAATCAGACA GTGAAGAGGA TGAAGAGTCT TTATTGGAGC AGGATCAGAG GCCACCCAAG 
CAGAGTGCTT TTCAGCTTGC ATACCAGGCA TGGGTTACAA GTGCCAAATC TGCGCTAAAG 
GAAAGAAAGA GTCGGCAGAA AGAGAGATCT GGAAAGGTAG AAATGGTCAC GGTAGAGAAT 
AATGAAGCAG GAGGGGGGAC AGAGTCTCAT ACCCAGGAAC CAAGGGAGAA AGGAGAGGAT 
AAAGATCTGA ATGAGGAGAG AGGAGGAAGC AATGTGGTAC AGCGTATCCT TGACGTGTTG 
AAGTTTCTCT GGGTTCTTTT GCTTGCCATG GTGGATGGAC TGATCAATTG GCTAGGAACC 
CTTACAAAGC AGCATGTTGA TATGTCCACA GTACTGTGGG TAGAGAGATA TATCATAACC 
CAAAAACTGT CTAAGGGAGA AGATATCAAC CGCAGGGATT TGGAAAAGGT ATATGAGACA 
GGGCAGGATG TTAGGGAGCC TAGGATTTCA TCTCCTCCAA CTGATATGTC AACTAGCATT 
GCTTCAAGTG GCTGTGAGGA AGAGGAGCTT ATCTGCAGCA CAGCAGAGTG TACGGAAAAT 
ATAACACAAC TCAGCACCGG GTACAACACA CGAAGTAACA GCTTGGACTC TATTAGGGGG 
TTTCCGGAAA TCAAGCCAAG TCATCGTTCT CGTACTGCTA GTGAATTGCT GCTTTCCAGG 
CCACTTGATT TCCCAGAGCT CTCCGAGTCT CAGCACTTCT TTCAGTCTCA GAGTCGTATT 
CTGAGGCTCC TGTTGGCCCT CTATAACTTT GTGGCTGCTC ACTCTGAGCT GCTTTGTTAC 
TTCATCATCA TTCTCAACAA CATGGTGAGC GCTTCCCTGA TATCACTGTT CCTTCCAATT 
CTCATCTTCC TGTGGGGTAT GCTGTCCATC CACAAACCCA GTAAGCGCTT CTGGATGACT 
GCTATCATTT TTACTGAGGT TATGGTAGTA ATAAAATACC TCTTCCAGTT TGGTTTCTTC 
CCATGGAATA ATTATACCAT TCTGGTACGC AACGAAGGGA AACCATTTTT TCCTCCACGT 
GTCCTGGGAC TTGAGAAGAG TGACTCGTAC ATCAAATATG ATCTGATCCA GCTCTTGATG 
CTCTTTTTCC ATCGCTCACT GCTCATGAGT TATGGTCTTT GGGATCGTAA AGACTTAGAC 
CTTGAAGAGA GAACACCCGG GAAGGATAAA GGGAGCAAAA CAGAGATTCA GGCATCCCAG 
GATCTGGAGC CAGCAGGAGG CAGTAGCTCT TCCCTGCGTC AGCGCAAAAG AAAAGAATCC 
CAGGGCAGTG TGCAAGAAAG AGATTCCTTA AAAGAGAAAT CACTAGATGT GCAGAAGAAG 
AAAAAAGGAA GAGTGGCTCT TAGGGTGAAG ATCATGGGAA TGAAAATGCA ACAGTTCTTT 
GCCTGCATAG TGCGGAATAT ATATCAGCCA GTGAAAGCTT TTTTCCATGA TATTCTACAT 
ACCAAGAACC GTGCTGCAAC AGATGTTTAT GCCTTCATGT TCCTCGCAGA TGTGGTTGAC 
TTTGTGATCA TCGTATTTGG GTTTTGGGCT TTTGGAAAAC ACTCTGCTGC TGCCGACATA 
GCATCATCTC TCTCTGATGA CCAAGTACCA GAAGCTTTCC TAGTCATGGT CCTCATACAG 
TTTTCCACCA TGGTGATAGA TCGTGCTCTA TACCTGCGGA AGACTGTGCT TGGAAAGCTC 
ATCTTTCAAG TTATTCTGGT GATTGGGATA CATGTCTGGA TGTTCTTTAT CCTACCAGCA 
GTCACAGAAA GGATGTTCAA TCAGAACACT GTGGCCCAGC TCTGGTATTT TGTTAAGTGC 
ATTTACTTTG CCCTGTCTGC CTACCAGATC CGCTGTGGGT ATCCTACCCG CATCCTGGGC 
AACTTCCTTA CCAAGAAATA CAACCACCTC AACCTATTCC TATTTCAGGG GTTTCGCTTG 
GTTCCGTTTC TAGTAGAGCT CAGGGCTGTA ATGGACTGGG TTTGGACTGA TACCACCTTG 
TCCTTGTCCA ACTGGATGTG CGTGGAAGAC ATTTATGCTA ATATCTTCAT TATCAAATGC 
AGCCGAGAGA CTGAAAAGAA ATACCCTCAG CCTAAAGGAC AGAAAAAAAA GAAGATAGTG 
AAATATGGAA TGGGTGGTTT GATCATAGTC TTCCTTATCT GTATAATCTG GTTTCCCCTT 
CTCTTCATGT CGTTGGTGCG CTCAGTGGTG GGAGTCGTCA ACCACCCTAT CGATGTCACG 
GTTACTTTTA AACTCGGGGG TTATGAGCCT TTATTCACGA TGAGTGCACA GCAACAGTCC 
ATCCAGCCAT TCACCCCACA GCAATATGAA GCGCTTACCT ATGAGTTTGA GAGACAGCCG 
ACTGCCATGC AGTTCATTAC TTTGTATAGT TATGAGGACA TAGTGACTGC GCGAATTGAG 
GGAAGTTCAG GATCTGTATG GGGAATCAGC CCTCCAAGCC GTGAGCAGAT GAGAAAGGAG 
CTGCAAAATG GATCCTCTGA CATTACACTA CGCTTCACCT GGGATTTTCA GAGGGATCTG 
GCAAAAGGTG GCACTGTGGA GCACACATCT GAGAAACACA CTAAGGACTT GGAGCCTGGG 
AGCCAGGAGA GGATGGAGCT GGCAGATCTT CTTCAAGGAG TACGAGACAC CCCTGTCTCT 
ATCCAGCACT TGTTTCCAAA GTACATCCGC GCTCCTAATG GCCCAGAAGC AAATCCAGTG 
AAACAGCTCC AGCCAGATGA AGAAGAAAGT TACCTGGATG TGGAGGTGCA GCTAAATCGG 
ATGCGAGTTG CAGAGGGAAA TAGCACCAGC AGTTTTGTGG AGTGGTGGGT TATTACTCTA 
AAAGACTGCG AGGGAGAAGA CTGCAACATA CTACCTATGG TGATTTTTAA CGATAAAGTT 
AGCCCTCCTA GCTTAGGATT CCTGGCTGGA TATGGGATAA TGGGTTTGTA TGTATCTATT 
GTCCTGGTCA TTGGGAAGTT TGTGCGCGGC TTCTTCAGTG AAATCTCTCA CTCCATCATG 
TTTGAGGAGC TGCCTTGTGT GGATCGGATT CTCAAGCTGT GCCAGGATAT CTATGTGGTG 
CGGGAGCGTG GGGAATTGGA ACTGGAAGAG GAACTCTATG CAAAACTCAT CTTCCTGTAT 
CGCTCACCAG AAACCATGAT CAAGTGGACT GTAGATAAGG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002933721.3
CDS	820..8244
Translation	MELQVLCGVLFWLLLPGSLLAACLFRYNSLSLVYLFYLLLLPWFAGPNPCSMRGHTGRLMKALLATSILFLCAHLIFQICLYTIPELDDLLGHNCSSWDVLARHVGINRLDLSDIPNMVRQVSPDFGMFVVSALCLGLCNRLVTKKRRQRSSGYFEQEEERTELNLKYESRECDTEFEVGGDLHSVPRDRLLGSWLRKAAYRLLETVEKSLAVLLLALAGITDPSAFSAPYYVVFIGLCTWWACHFPISQLGFNTICVIVGFFTGGHMICLYIYQTPFIQQMFHPEQLWARLFGLHNIISHSNITDCTHDSLNQNLDWPVYVHPGILLLLYYSLLSILKLKSSDKKRISGCDLRKSQQEDMELTDIEPVSKEQYSAGEETKPMLQRMNTEEEAKPQDCTVHVIRRNSNQLPEELETKEPSQFHLMIHVILSQSYICALIAMMVWSITYHSWLTFVLLLWACLIWMVRNRRQFAMLCSPFILMYGVTLCSLQYVWSMDLREELPTQLGFMKLSQLGLVRAPYPCLNMGALLLYTLTFWLLLHQFVTEKFLKRKKFMSPLLEVTVADAGKEKNLLKRFGEQVKSFYAKYWIYVCGGMFIVVSFAGRLVVYKIVYMLLFLLCLVLFQVYYSLWRKLLTFFWWLVVAYTMLVLITIYTFQFEEFPEYWKNLTNLTDEQLGDIGLERFSVSELFTSILIPGFFLLACILQLHYFHRPFMHMTNLAHASCQGSFHSHESEQPKEEEEKAGACSDAAADEEEDEEESAHGADLSKWDLVTQRLSVLSFKFSELLHRLQVFLWRLLELHVLKLVALYSVWVALQEVSVMNFLFVVLWAFAMPFSRFQHMASCLCTIWTSIIIVCKMLYQLTVVNPLEYSSNCTQPPLNNTSLLEDEILNSTLYREPIDPANWFGIRKGYPNLGYIQNHLQILLLLVFEAIVYRHQEFYRKQYQLEKPHIQAVFPDVYREHLDQGLFSCIKYFINYSFYKFGLEICFLLTVNVIGQRMNFMVIFHGCWLIVILTRRQRVNIAKLWPKYCVFLIFFLLWQYMICLGIPPVLCIDYPWRWDTFVPVNSALIKWLYLPDFYTTPKSVNLISDFVLLIGVAQQWKVFINEKKEEWYTMAGENVEEYEAPKDRPNPVPNFINCQSYLDMVKVAVFRYLFWFVLTVVFITGATRISVFGLGYLMSCFYLLLSGTTLLQKPMKAKLVLWDCLILYNITVILSKNMLSLLSCVFVQQMQTNFCWVIQLFSLVCTVKGYYNPQDMDKDRDCSLPVEEAGIIWDSICFLFLLLQRRVFLSCYFRHVVLDLRASSLQTSRGFELFMASIRKNVFFHQESERRSLNQLRKQMERIRAKQERYHEGRRQTDSSQVKEGEPGSKKGSSRQKIHWSRPWLDHASVLHSGDYFLFESDSEEDEESLLEQDQRPPKQSAFQLAYQAWVTSAKSALKERKSRQKERSGKVEMVTVENNEAGGGTESHTQEPREKGEDKDLNEERGGSNVVQRILDVLKFLWVLLLAMVDGLINWLGTLTKQHVDMSTVLWVERYIITQKLSKGEDINRRDLEKVYETGQDVREPRISSPPTDMSTSIASSGCEEEELICSTAECTENITQLSTGYNTRSNSLDSIRGFPEIKPSHRSRTASELLLSRPLDFPELSESQHFFQSQSRILRLLLALYNFVAAHSELLCYFIIILNNMVSASLISLFLPILIFLWGMLSIHKPSKRFWMTAIIFTEVMVVIKYLFQFGFFPWNNYTILVRNEGKPFFPPRVLGLEKSDSYIKYDLIQLLMLFFHRSLLMSYGLWDRKDLDLEERTPGKDKGSKTEIQASQDLEPAGGSSSSLRQRKRKESQGSVQERDSLKEKSLDVQKKKKGRVALRVKIMGMKMQQFFACIVRNIYQPVKAFFHDILHTKNRAATDVYAFMFLADVVDFVIIVFGFWAFGKHSAAADIASSLSDDQVPEAFLVMVLIQFSTMVIDRALYLRKTVLGKLIFQVILVIGIHVWMFFILPAVTERMFNQNTVAQLWYFVKCIYFALSAYQIRCGYPTRILGNFLTKKYNHLNLFLFQGFRLVPFLVELRAVMDWVWTDTTLSLSNWMCVEDIYANIFIIKCSRETEKKYPQPKGQKKKKIVKYGMGGLIIVFLICIIWFPLLFMSLVRSVVGVVNHPIDVTVTFKLGGYEPLFTMSAQQQSIQPFTPQQYEALTYEFERQPTAMQFITLYSYEDIVTARIEGSSGSVWGISPPSREQMRKELQNGSSDITLRFTWDFQRDLAKGGTVEHTSEKHTKDLEPGSQERMELADLLQGVRDTPVSIQHLFPKYIRAPNGPEANPVKQLQPDEEESYLDVEVQLNRMRVAEGNSTSSFVEWWVITLKDCEGEDCNILPMVIFNDKVSPPSLGFLAGYGIMGLYVSIVLVIGKFVRGFFSEISHSIMFEELPCVDRILKLCQDIYVVRERGELELEEELYAKLIFLYRSPETMIKWTVDKD

Target ORF information:

RefSeq Version	XM_002933675.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis piezo-type mechanosensitive ion channel component 1 (piezo1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002933675.5

ORF Insert Sequence:

ATGGAACTGC AAGTCCTCTG TGGGGTCCTG TTTTGGCTCC TTCTGCCCGG CTCCCTCTTG 
GCAGCCTGCT TATTCCGTTA CAACTCCTTG TCACTGGTGT ACCTGTTTTA TCTTCTGCTT 
CTGCCATGGT TTGCTGGACC AAATCCCTGC AGCATGAGAG GTCACACAGG ACGTCTGATG 
AAAGCGTTAC TTGCTACCAG CATCCTCTTC CTCTGTGCTC ATCTCATCTT CCAGATCTGC 
CTGTACACAA TACCTGAACT GGATGACCTG CTAGGGCACA ACTGCAGCAG CTGGGATGTG 
TTGGCTCGTC ACGTAGGTAT AAACAGGCTG GATCTTTCTG ATATCCCCAA CATGGTGCGA 
CAAGTGTCCC CAGATTTTGG CATGTTTGTC GTGTCAGCAT TGTGCCTTGG CTTGTGTAAT 
CGCCTAGTGA CCAAGAAGAG ACGACAGAGG TCTTCAGGCT ATTTTGAGCA GGAAGAAGAG 
AGGACTGAAC TTAACCTAAA ATATGAAAGT AGAGAGTGTG ATACAGAGTT TGAGGTGGGG 
GGAGACTTAC ATTCTGTTCC ACGAGACCGC CTTCTTGGGT CCTGGCTTAG AAAGGCAGCA 
TACAGGCTTC TGGAAACAGT GGAGAAATCA CTTGCTGTCT TACTCTTGGC TCTTGCAGGT 
ATCACTGATC CCTCTGCTTT CTCAGCCCCA TATTATGTGG TTTTCATTGG TCTCTGCACA 
TGGTGGGCCT GCCATTTTCC CATTAGCCAA CTAGGCTTTA ATACTATCTG TGTCATTGTT 
GGGTTCTTCA CTGGAGGTCA CATGATTTGC CTTTATATTT ACCAGACACC TTTTATCCAA 
CAAATGTTTC ATCCTGAACA GTTATGGGCA AGGTTGTTTG GTCTGCATAA CATCATCAGT 
CACTCCAACA TTACAGACTG TACCCATGAC TCTCTGAATC AAAACCTTGA CTGGCCTGTG 
TATGTTCATC CCGGAATCTT ATTACTGCTG TACTACTCCC TATTATCAAT ACTGAAACTG 
AAGAGCTCTG ACAAGAAAAG GATTTCAGGG TGTGACCTTC GGAAATCTCA GCAGGAGGAT 
ATGGAGTTGA CTGATATTGA GCCAGTTTCA AAGGAGCAAT ATAGTGCGGG AGAAGAGACA 
AAACCAATGC TTCAGAGGAT GAACACTGAA GAAGAGGCTA AACCCCAAGA CTGCACTGTT 
CATGTGATTA GAAGAAATTC TAATCAACTG CCAGAAGAAC TTGAAACCAA GGAGCCATCA 
CAATTTCACC TGATGATTCA TGTAATACTG AGCCAAAGCT ATATCTGTGC GCTTATTGCA 
ATGATGGTGT GGAGCATTAC ATATCACAGC TGGCTGACTT TTGTGCTGCT GCTCTGGGCA 
TGTTTAATCT GGATGGTGAG GAACAGGCGC CAGTTTGCAA TGCTGTGCTC TCCCTTTATT 
TTGATGTATG GAGTGACTCT CTGCAGCCTG CAGTATGTCT GGAGTATGGA CTTGAGAGAA 
GAGCTTCCGA CACAACTTGG TTTTATGAAG CTGTCACAGC TGGGACTGGT TCGCGCACCA 
TACCCCTGCC TTAACATGGG AGCACTGCTA CTTTACACGC TGACATTTTG GCTTCTGCTG 
CACCAATTTG TAACAGAAAA ATTTCTAAAA CGTAAGAAGT TTATGTCCCC ACTCCTGGAG 
GTCACAGTGG CAGATGCTGG CAAAGAAAAG AACCTGCTAA AACGTTTTGG GGAGCAAGTA 
AAAAGCTTCT ATGCAAAATA CTGGATCTAT GTGTGTGGAG GCATGTTTAT TGTGGTCAGC 
TTTGCTGGTA GGCTGGTAGT TTACAAAATA GTCTACATGC TTCTGTTCCT ACTATGCTTG 
GTGTTATTTC AGGTGTATTA CTCTTTGTGG AGGAAGCTGC TGACTTTTTT CTGGTGGTTA 
GTTGTAGCAT ACACAATGTT GGTTCTTATC ACCATCTACA CCTTTCAATT TGAAGAGTTT 
CCTGAGTACT GGAAAAACTT GACCAACCTT ACAGATGAGC AGTTGGGTGA TATTGGGCTT 
GAGAGGTTTA GTGTGTCGGA ACTTTTTACC AGCATCCTTA TCCCAGGCTT CTTCCTCCTT 
GCCTGCATCC TCCAGTTACA CTACTTTCAT CGCCCTTTTA TGCACATGAC CAACTTGGCA 
CATGCCTCAT GCCAAGGAAG TTTCCATAGC CACGAGAGTG AGCAGCCAAA GGAAGAAGAA 
GAGAAAGCTG GTGCATGCAG TGATGCTGCT GCTGATGAGG AGGAGGATGA GGAAGAATCT 
GCACATGGAG CTGATCTAAG TAAATGGGAC CTAGTTACAC AGCGCCTTTC TGTCCTGTCC 
TTTAAGTTCT CTGAGCTGCT GCACCGATTA CAGGTTTTCC TTTGGAGGCT TTTGGAGCTC 
CATGTGTTGA AACTGGTGGC TTTGTATAGC GTGTGGGTTG CCCTGCAGGA GGTATCTGTG 
ATGAATTTCC TTTTTGTGGT CCTATGGGCT TTTGCTATGC CTTTCTCCCG ATTCCAGCAC 
ATGGCTTCCT GTCTTTGCAC CATCTGGACC TCTATAATTA TTGTTTGCAA AATGCTTTAC 
CAGCTTACTG TTGTCAACCC TCTGGAGTAC TCTAGCAACT GTACTCAGCC TCCTCTAAAT 
AACACCAGTC TCCTAGAAGA CGAGATTTTG AACTCCACCC TTTATAGAGA ACCTATAGAT 
CCAGCAAACT GGTTTGGTAT CAGAAAAGGT TATCCAAACC TAGGTTACAT ACAGAATCAC 
CTGCAAATAC TCCTACTTTT GGTATTTGAG GCAATTGTTT ATCGGCATCA GGAGTTTTAC 
CGTAAACAAT ACCAGCTTGA GAAGCCACAC ATTCAGGCTG TATTTCCAGA TGTCTACAGA 
GAGCACTTGG ACCAAGGCTT GTTCAGCTGT ATCAAATATT TTATCAACTA TTCCTTTTAT 
AAGTTTGGTC TTGAGATCTG CTTCCTTCTT ACTGTGAATG TGATTGGTCA GAGGATGAAC 
TTTATGGTAA TCTTTCATGG CTGCTGGCTG ATAGTGATTC TTACTCGACG ACAGCGAGTC 
AACATTGCAA AATTATGGCC TAAATACTGT GTGTTCCTAA TCTTTTTCCT GTTGTGGCAG 
TACATGATAT GTCTGGGCAT TCCTCCGGTG CTTTGTATAG ATTATCCCTG GAGGTGGGAC 
ACATTTGTTC CTGTCAACTC TGCACTGATA AAGTGGTTAT ATCTTCCAGA TTTCTATACT 
ACACCTAAAT CTGTCAACCT TATCAGTGAT TTTGTTTTGC TGATTGGTGT GGCCCAGCAG 
TGGAAGGTGT TTATTAATGA GAAGAAGGAA GAATGGTACA CTATGGCAGG GGAAAATGTG 
GAAGAGTATG AAGCTCCAAA AGACAGGCCC AACCCTGTAC CTAACTTCAT CAATTGTCAG 
TCTTATCTGG ATATGGTGAA AGTAGCAGTG TTTCGGTACC TATTCTGGTT TGTCTTGACT 
GTGGTCTTTA TAACAGGGGC CACTCGCATC AGTGTTTTTG GCCTAGGATA TTTGATGTCC 
TGTTTCTACC TTCTTCTGTC TGGCACAACC CTTCTGCAAA AGCCAATGAA AGCCAAGCTG 
GTGCTTTGGG ACTGTTTGAT CCTCTACAAC ATCACTGTTA TACTGTCCAA GAACATGCTG 
TCGCTCCTGT CTTGTGTATT TGTTCAACAA ATGCAAACCA ACTTTTGCTG GGTGATCCAG 
CTCTTCAGCC TAGTTTGCAC AGTTAAGGGT TACTACAATC CCCAAGATAT GGATAAAGAC 
CGGGATTGCT CTCTGCCAGT GGAAGAGGCC GGAATCATTT GGGATAGCAT CTGCTTCTTA 
TTTCTCCTTC TCCAACGAAG AGTTTTTTTG AGTTGCTACT TTCGGCATGT TGTTCTTGAC 
CTTCGTGCCT CCTCCCTTCA AACATCAAGA GGTTTTGAGC TCTTCATGGC CAGCATCAGA 
AAGAATGTGT TTTTCCACCA AGAATCTGAG AGACGATCTC TTAACCAGCT CAGGAAGCAA 
ATGGAGAGAA TCCGAGCAAA GCAAGAACGC TATCATGAAG GCAGAAGACA AACTGATAGC 
AGTCAAGTAA AGGAAGGTGA GCCTGGCAGC AAGAAAGGGT CCAGTAGGCA AAAGATTCAT 
TGGAGCCGCC CATGGTTAGA CCACGCCTCA GTGCTGCATT CAGGAGATTA TTTTCTGTTC 
GAATCAGACA GTGAAGAGGA TGAAGAGTCT TTATTGGAGC AGGATCAGAG GCCACCCAAG 
CAGAGTGCTT TTCAGCTTGC ATACCAGGCA TGGGTTACAA GTGCCAAATC TGCGCTAAAG 
GAAAGAAAGA GTCGGCAGAA AGAGAGATCT GGAAAGGTAG AAATGGTCAC GGTAGAGAAT 
AATGAAGCAG GAGGGGGGAC AGAGTCTCAT ACCCAGGAAC CAAGGGAGAA AGGAGAGGAT 
AAAGATCTGA ATGAGGAGAG AGGAGGAAGC AATGTGGTAC AGCGTATCCT TGACGTGTTG 
AAGTTTCTCT GGGTTCTTTT GCTTGCCATG GTGGATGGAC TGATCAATTG GCTAGGAACC 
CTTACAAAGC AGCATGTTGA TATGTCCACA GTACTGTGGG TAGAGAGATA TATCATAACC 
CAAAAACTGT CTAAGGGAGA AGATATCAAC CGCAGGGATT TGGAAAAGGT ATATGAGACA 
GGGCAGGATG TTAGGGAGCC TAGGATTTCA TCTCCTCCAA CTGATATGTC AACTAGCATT 
GCTTCAAGTG GCTGTGAGGA AGAGGAGCTT ATCTGCAGCA CAGCAGAGTG TACGGAAAAT 
ATAACACAAC TCAGCACCGG GTACAACACA CGAAGTAACA GCTTGGACTC TATTAGGGGG 
TTTCCGGAAA TCAAGCCAAG TCATCGTTCT CGTACTGCTA GTGAATTGCT GCTTTCCAGG 
CCACTTGATT TCCCAGAGCT CTCCGAGTCT CAGCACTTCT TTCAGTCTCA GAGTCGTATT 
CTGAGGCTCC TGTTGGCCCT CTATAACTTT GTGGCTGCTC ACTCTGAGCT GCTTTGTTAC 
TTCATCATCA TTCTCAACAA CATGGTGAGC GCTTCCCTGA TATCACTGTT CCTTCCAATT 
CTCATCTTCC TGTGGGGTAT GCTGTCCATC CACAAACCCA GTAAGCGCTT CTGGATGACT 
GCTATCATTT TTACTGAGGT TATGGTAGTA ATAAAATACC TCTTCCAGTT TGGTTTCTTC 
CCATGGAATA ATTATACCAT TCTGGTACGC AACGAAGGGA AACCATTTTT TCCTCCACGT 
GTCCTGGGAC TTGAGAAGAG TGACTCGTAC ATCAAATATG ATCTGATCCA GCTCTTGATG 
CTCTTTTTCC ATCGCTCACT GCTCATGAGT TATGGTCTTT GGGATCGTAA AGACTTAGAC 
CTTGAAGAGA GAACACCCGG GAAGGATAAA GGGAGCAAAA CAGAGATTCA GGCATCCCAG 
GATCTGGAGC CAGCAGGAGG CAGTAGCTCT TCCCTGCGTC AGCGCAAAAG AAAAGAATCC 
CAGGGCAGTG TGCAAGAAAG AGATTCCTTA AAAGAGAAAT CACTAGATGT GCAGAAGAAG 
AAAAAAGGAA GAGTGGCTCT TAGGGTGAAG ATCATGGGAA TGAAAATGCA ACAGTTCTTT 
GCCTGCATAG TGCGGAATAT ATATCAGCCA GTGAAAGCTT TTTTCCATGA TATTCTACAT 
ACCAAGAACC GTGCTGCAAC AGATGTTTAT GCCTTCATGT TCCTCGCAGA TGTGGTTGAC 
TTTGTGATCA TCGTATTTGG GTTTTGGGCT TTTGGAAAAC ACTCTGCTGC TGCCGACATA 
GCATCATCTC TCTCTGATGA CCAAGTACCA GAAGCTTTCC TAGTCATGGT CCTCATACAG 
TTTTCCACCA TGGTGATAGA TCGTGCTCTA TACCTGCGGA AGACTGTGCT TGGAAAGCTC 
ATCTTTCAAG TTATTCTGGT GATTGGGATA CATGTCTGGA TGTTCTTTAT CCTACCAGCA 
GTCACAGAAA GGATGTTCAA TCAGAACACT GTGGCCCAGC TCTGGTATTT TGTTAAGTGC 
ATTTACTTTG CCCTGTCTGC CTACCAGATC CGCTGTGGGT ATCCTACCCG CATCCTGGGC 
AACTTCCTTA CCAAGAAATA CAACCACCTC AACCTATTCC TATTTCAGGG GTTTCGCTTG 
GTTCCGTTTC TAGTAGAGCT CAGGGCTGTA ATGGACTGGG TTTGGACTGA TACCACCTTG 
TCCTTGTCCA ACTGGATGTG CGTGGAAGAC ATTTATGCTA ATATCTTCAT TATCAAATGC 
AGCCGAGAGA CTGAAAAGAA ATACCCTCAG CCTAAAGGAC AGAAAAAAAA GAAGATAGTG 
AAATATGGAA TGGGTGGTTT GATCATAGTC TTCCTTATCT GTATAATCTG GTTTCCCCTT 
CTCTTCATGT CGTTGGTGCG CTCAGTGGTG GGAGTCGTCA ACCACCCTAT CGATGTCACG 
GTTACTTTTA AACTCGGGGG TTATGAGCCT TTATTCACGA TGAGTGCACA GCAACAGTCC 
ATCCAGCCAT TCACCCCACA GCAATATGAA GCGCTTACCT ATGAGTTTGA GAGACAGCCG 
ACTGCCATGC AGTTCATTAC TTTGTATAGT TATGAGGACA TAGTGACTGC GCGAATTGAG 
GGAAGTTCAG GATCTGTATG GGGAATCAGC CCTCCAAGCC GTGAGCAGAT GAGAAAGGAG 
CTGCAAAATG GATCCTCTGA CATTACACTA CGCTTCACCT GGGATTTTCA GAGGGATCTG 
GCAAAAGGTG GCACTGTGGA GCACACATCT GAGAAACACA CTAAGGACTT GGAGCCTGGG 
AGCCAGGAGA GGATGGAGCT GGCAGATCTT CTTCAAGGAG TACGAGACAC CCCTGTCTCT 
ATCCAGCACT TGTTTCCAAA GTACATCCGC GCTCCTAATG GCCCAGAAGC AAATCCAGTG 
AAACAGCTCC AGCCAGATGA AGAAGAAAGT TACCTGGATG TGGAGGTGCA GCTAAATCGG 
ATGCGAGTTG CAGAGGGAAA TAGCACCAGC AGTTTTGTGG AGTGGTGGGT TATTACTCTA 
AAAGACTGCG AGGGAGAAGA CTGCAACATA CTACCTATGG TGATTTTTAA CGATAAAGTT 
AGCCCTCCTA GCTTAGGATT CCTGGCTGGA TATGGGATAA TGGGTTTGTA TGTATCTATT 
GTCCTGGTCA TTGGGAAGTT TGTGCGCGGC TTCTTCAGTG AAATCTCTCA CTCCATCATG 
TTTGAGGAGC TGCCTTGTGT GGATCGGATT CTCAAGCTGT GCCAGGATAT CTATGTGGTG 
CGGGAGCGTG GGGAATTGGA ACTGGAAGAG GAACTCTATG CAAAACTCAT CTTCCTGTAT 
CGCTCACCAG AAACCATGAT CAAGTGGACT GTAGATAAGG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

piezo1 ( XM_012962280.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817734.2 Xenopus tropicalis piezo-type mechanosensitive ion channel component 1 (piezo1), transcript variant X2, mRNA.

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CloneID	OXa56851
Clone ID Related Accession (Same CDS sequence)	XM_012962280.2
Accession Version	XM_012962280.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	piezo-type mechanosensitive ion channel component 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012962280.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACTGC AAGTCCTCTG TGGGGTCCTG TTTTGGCTCC TTCTGCCCGG CTCCCTCTTG 
GCAGCCTGCT TATTCCGTTA CAACTCCTTG TCACTGGTGT ACCTGTTTTA TCTTCTGCTT 
CTGCCATGGT TTGCTGGACC AAATCCCTGC AGCATGAGAG GTCACACAGG ACGTCTGATG 
AAAGCGTTAC TTGCTACCAG CATCCTCTTC CTCTGTGCTC ATCTCATCTT CCAGATCTGC 
CTGTACACAA TACCTGAACT GGATGACCTG CTAGGGCACA ACTGCAGCAG CTGGGATGTG 
TTGGCTCGTC ACGTAGGTAT AAACAGGCTG GATCTTTCTG ATATCCCCAA CATGGTGCGA 
CAAGTGTCCC CAGATTTTGG CATGTTTGTC GTGTCAGCAT TGTGCCTTGG CTTGTGTAAT 
CGCCTAGTGA CCAAGAAGAG ACGACAGAGG TCTTCAGGCT ATTTTGAGCA GGAAGAAGAG 
AGGACTGAAC TTAACCTAAA ATATGAAAGT AGAGAGTGTG ATACAGAGTT TGAGGTGGGG 
GGAGACTTAC ATTCTGTTCC ACGAGACCGC CTTCTTGGGT CCTGGCTTAG AAAGGCAGCA 
TACAGGCTTC TGGAAACAGT GGAGAAATCA CTTGCTGTCT TACTCTTGGC TCTTGCAGGT 
ATCACTGATC CCTCTGCTTT CTCAGCCCCA TATTATGTGG TTTTCATTGG TCTCTGCACA 
TGGTGGGCCT GCCATTTTCC CATTAGCCAA CTAGGCTTTA ATACTATCTG TGTCATTGTT 
GGGTTCTTCA CTGGAGGTCA CATGATTTGC CTTTATATTT ACCAGACACC TTTTATCCAA 
CAAATGTTTC ATCCTGAACA GTTATGGGCA AGGTTGTTTG GTCTGCATAA CATCATCAGT 
CACTCCAACA TTACAGACTG TACCCATGAC TCTCTGAATC AAAACCTTGA CTGGCCTGTG 
TATGTTCATC CCGGAATCTT ATTACTGCTG TACTACTCCC TATTATCAAT ACTGAAACTG 
AAGAGCTCTG ACAAGAAAAG GATTTCAGGG TGTGACCTTC GGAAATCTCA GCAGGAGGAT 
ATGGAGTTGA CTGATATTGA GCCAGTTTCA AAGGAGCAAT ATAGTGCGGG AGAAGAGACA 
AAACCAATGC TTCAGAGGAT GAACACTGAA GAAGAGGCTA AACCCCAAGA CTGCACTGTT 
CATGTGATTA GAAGAAATTC TAATCAACTG CCAGAACTTG AAACCAAGGA GCCATCACAA 
TTTCACCTGA TGATTCATGT AATACTGAGC CAAAGCTATA TCTGTGCGCT TATTGCAATG 
ATGGTGTGGA GCATTACATA TCACAGCTGG CTGACTTTTG TGCTGCTGCT CTGGGCATGT 
TTAATCTGGA TGGTGAGGAA CAGGCGCCAG TTTGCAATGC TGTGCTCTCC CTTTATTTTG 
ATGTATGGAG TGACTCTCTG CAGCCTGCAG TATGTCTGGA GTATGGACTT GAGAGAAGAG 
CTTCCGACAC AACTTGGTTT TATGAAGCTG TCACAGCTGG GACTGGTTCG CGCACCATAC 
CCCTGCCTTA ACATGGGAGC ACTGCTACTT TACACGCTGA CATTTTGGCT TCTGCTGCAC 
CAATTTGTAA CAGAAAAATT TCTAAAACGT AAGAAGTTTA TGTCCCCACT CCTGGAGGTC 
ACAGTGGCAG ATGCTGGCAA AGAAAAGAAC CTGCTAAAAC GTTTTGGGGA GCAAGTAAAA 
AGCTTCTATG CAAAATACTG GATCTATGTG TGTGGAGGCA TGTTTATTGT GGTCAGCTTT 
GCTGGTAGGC TGGTAGTTTA CAAAATAGTC TACATGCTTC TGTTCCTACT ATGCTTGGTG 
TTATTTCAGG TGTATTACTC TTTGTGGAGG AAGCTGCTGA CTTTTTTCTG GTGGTTAGTT 
GTAGCATACA CAATGTTGGT TCTTATCACC ATCTACACCT TTCAATTTGA AGAGTTTCCT 
GAGTACTGGA AAAACTTGAC CAACCTTACA GATGAGCAGT TGGGTGATAT TGGGCTTGAG 
AGGTTTAGTG TGTCGGAACT TTTTACCAGC ATCCTTATCC CAGGCTTCTT CCTCCTTGCC 
TGCATCCTCC AGTTACACTA CTTTCATCGC CCTTTTATGC ACATGACCAA CTTGGCACAT 
GCCTCATGCC AAGGAAGTTT CCATAGCCAC GAGAGTGAGC AGCCAAAGGA AGAAGAAGAG 
AAAGCTGGTG CATGCAGTGA TGCTGCTGCT GATGAGGAGG AGGATGAGGA AGAATCTGCA 
CATGGAGCTG ATCTAAGTAA ATGGGACCTA GTTACACAGC GCCTTTCTGT CCTGTCCTTT 
AAGTTCTCTG AGCTGCTGCA CCGATTACAG GTTTTCCTTT GGAGGCTTTT GGAGCTCCAT 
GTGTTGAAAC TGGTGGCTTT GTATAGCGTG TGGGTTGCCC TGCAGGAGGT ATCTGTGATG 
AATTTCCTTT TTGTGGTCCT ATGGGCTTTT GCTATGCCTT TCTCCCGATT CCAGCACATG 
GCTTCCTGTC TTTGCACCAT CTGGACCTCT ATAATTATTG TTTGCAAAAT GCTTTACCAG 
CTTACTGTTG TCAACCCTCT GGAGTACTCT AGCAACTGTA CTCAGCCTCC TCTAAATAAC 
ACCAGTCTCC TAGAAGACGA GATTTTGAAC TCCACCCTTT ATAGAGAACC TATAGATCCA 
GCAAACTGGT TTGGTATCAG AAAAGGTTAT CCAAACCTAG GTTACATACA GAATCACCTG 
CAAATACTCC TACTTTTGGT ATTTGAGGCA ATTGTTTATC GGCATCAGGA GTTTTACCGT 
AAACAATACC AGCTTGAGAA GCCACACATT CAGGCTGTAT TTCCAGATGT CTACAGAGAG 
CACTTGGACC AAGGCTTGTT CAGCTGTATC AAATATTTTA TCAACTATTC CTTTTATAAG 
TTTGGTCTTG AGATCTGCTT CCTTCTTACT GTGAATGTGA TTGGTCAGAG GATGAACTTT 
ATGGTAATCT TTCATGGCTG CTGGCTGATA GTGATTCTTA CTCGACGACA GCGAGTCAAC 
ATTGCAAAAT TATGGCCTAA ATACTGTGTG TTCCTAATCT TTTTCCTGTT GTGGCAGTAC 
ATGATATGTC TGGGCATTCC TCCGGTGCTT TGTATAGATT ATCCCTGGAG GTGGGACACA 
TTTGTTCCTG TCAACTCTGC ACTGATAAAG TGGTTATATC TTCCAGATTT CTATACTACA 
CCTAAATCTG TCAACCTTAT CAGTGATTTT GTTTTGCTGA TTGGTGTGGC CCAGCAGTGG 
AAGGTGTTTA TTAATGAGAA GAAGGAAGAA TGGTACACTA TGGCAGGGGA AAATGTGGAA 
GAGTATGAAG CTCCAAAAGA CAGGCCCAAC CCTGTACCTA ACTTCATCAA TTGTCAGTCT 
TATCTGGATA TGGTGAAAGT AGCAGTGTTT CGGTACCTAT TCTGGTTTGT CTTGACTGTG 
GTCTTTATAA CAGGGGCCAC TCGCATCAGT GTTTTTGGCC TAGGATATTT GATGTCCTGT 
TTCTACCTTC TTCTGTCTGG CACAACCCTT CTGCAAAAGC CAATGAAAGC CAAGCTGGTG 
CTTTGGGACT GTTTGATCCT CTACAACATC ACTGTTATAC TGTCCAAGAA CATGCTGTCG 
CTCCTGTCTT GTGTATTTGT TCAACAAATG CAAACCAACT TTTGCTGGGT GATCCAGCTC 
TTCAGCCTAG TTTGCACAGT TAAGGGTTAC TACAATCCCC AAGATATGGA TAAAGACCGG 
GATTGCTCTC TGCCAGTGGA AGAGGCCGGA ATCATTTGGG ATAGCATCTG CTTCTTATTT 
CTCCTTCTCC AACGAAGAGT TTTTTTGAGT TGCTACTTTC GGCATGTTGT TCTTGACCTT 
CGTGCCTCCT CCCTTCAAAC ATCAAGAGGT TTTGAGCTCT TCATGGCCAG CATCAGAAAG 
AATGTGTTTT TCCACCAAGA ATCTGAGAGA CGATCTCTTA ACCAGCTCAG GAAGCAAATG 
GAGAGAATCC GAGCAAAGCA AGAACGCTAT CATGAAGGCA GAAGACAAAC TGATAGCAGT 
CAAGTAAAGG AAGGTGAGCC TGGCAGCAAG AAAGGGTCCA GTAGGCAAAA GATTCATTGG 
AGCCGCCCAT GGTTAGACCA CGCCTCAGTG CTGCATTCAG GAGATTATTT TCTGTTCGAA 
TCAGACAGTG AAGAGGATGA AGAGTCTTTA TTGGAGCAGG ATCAGAGGCC ACCCAAGCAG 
AGTGCTTTTC AGCTTGCATA CCAGGCATGG GTTACAAGTG CCAAATCTGC GCTAAAGGAA 
AGAAAGAGTC GGCAGAAAGA GAGATCTGGA AAGGTAGAAA TGGTCACGGT AGAGAATAAT 
GAAGCAGGAG GGGGGACAGA GTCTCATACC CAGGAACCAA GGGAGAAAGG AGAGGATAAA 
GATCTGAATG AGGAGAGAGG AGGAAGCAAT GTGGTACAGC GTATCCTTGA CGTGTTGAAG 
TTTCTCTGGG TTCTTTTGCT TGCCATGGTG GATGGACTGA TCAATTGGCT AGGAACCCTT 
ACAAAGCAGC ATGTTGATAT GTCCACAGTA CTGTGGGTAG AGAGATATAT CATAACCCAA 
AAACTGTCTA AGGGAGAAGA TATCAACCGC AGGGATTTGG AAAAGGTATA TGAGACAGGG 
CAGGATGTTA GGGAGCCTAG GATTTCATCT CCTCCAACTG ATATGTCAAC TAGCATTGCT 
TCAAGTGGCT GTGAGGAAGA GGAGCTTATC TGCAGCACAG CAGAGTGTAC GGAAAATATA 
ACACAACTCA GCACCGGGTA CAACACACGA AGTAACAGCT TGGACTCTAT TAGGGGGTTT 
CCGGAAATCA AGCCAAGTCA TCGTTCTCGT ACTGCTAGTG AATTGCTGCT TTCCAGGCCA 
CTTGATTTCC CAGAGCTCTC CGAGTCTCAG CACTTCTTTC AGTCTCAGAG TCGTATTCTG 
AGGCTCCTGT TGGCCCTCTA TAACTTTGTG GCTGCTCACT CTGAGCTGCT TTGTTACTTC 
ATCATCATTC TCAACAACAT GGTGAGCGCT TCCCTGATAT CACTGTTCCT TCCAATTCTC 
ATCTTCCTGT GGGGTATGCT GTCCATCCAC AAACCCAGTA AGCGCTTCTG GATGACTGCT 
ATCATTTTTA CTGAGGTTAT GGTAGTAATA AAATACCTCT TCCAGTTTGG TTTCTTCCCA 
TGGAATAATT ATACCATTCT GGTACGCAAC GAAGGGAAAC CATTTTTTCC TCCACGTGTC 
CTGGGACTTG AGAAGAGTGA CTCGTACATC AAATATGATC TGATCCAGCT CTTGATGCTC 
TTTTTCCATC GCTCACTGCT CATGAGTTAT GGTCTTTGGG ATCGTAAAGA CTTAGACCTT 
GAAGAGAGAA CACCCGGGAA GGATAAAGGG AGCAAAACAG AGATTCAGGC ATCCCAGGAT 
CTGGAGCCAG CAGGAGGCAG TAGCTCTTCC CTGCGTCAGC GCAAAAGAAA AGAATCCCAG 
GGCAGTGTGC AAGAAAGAGA TTCCTTAAAA GAGAAATCAC TAGATGTGCA GAAGAAGAAA 
AAAGGAAGAG TGGCTCTTAG GGTGAAGATC ATGGGAATGA AAATGCAACA GTTCTTTGCC 
TGCATAGTGC GGAATATATA TCAGCCAGTG AAAGCTTTTT TCCATGATAT TCTACATACC 
AAGAACCGTG CTGCAACAGA TGTTTATGCC TTCATGTTCC TCGCAGATGT GGTTGACTTT 
GTGATCATCG TATTTGGGTT TTGGGCTTTT GGAAAACACT CTGCTGCTGC CGACATAGCA 
TCATCTCTCT CTGATGACCA AGTACCAGAA GCTTTCCTAG TCATGGTCCT CATACAGTTT 
TCCACCATGG TGATAGATCG TGCTCTATAC CTGCGGAAGA CTGTGCTTGG AAAGCTCATC 
TTTCAAGTTA TTCTGGTGAT TGGGATACAT GTCTGGATGT TCTTTATCCT ACCAGCAGTC 
ACAGAAAGGA TGTTCAATCA GAACACTGTG GCCCAGCTCT GGTATTTTGT TAAGTGCATT 
TACTTTGCCC TGTCTGCCTA CCAGATCCGC TGTGGGTATC CTACCCGCAT CCTGGGCAAC 
TTCCTTACCA AGAAATACAA CCACCTCAAC CTATTCCTAT TTCAGGGGTT TCGCTTGGTT 
CCGTTTCTAG TAGAGCTCAG GGCTGTAATG GACTGGGTTT GGACTGATAC CACCTTGTCC 
TTGTCCAACT GGATGTGCGT GGAAGACATT TATGCTAATA TCTTCATTAT CAAATGCAGC 
CGAGAGACTG AAAAGAAATA CCCTCAGCCT AAAGGACAGA AAAAAAAGAA GATAGTGAAA 
TATGGAATGG GTGGTTTGAT CATAGTCTTC CTTATCTGTA TAATCTGGTT TCCCCTTCTC 
TTCATGTCGT TGGTGCGCTC AGTGGTGGGA GTCGTCAACC ACCCTATCGA TGTCACGGTT 
ACTTTTAAAC TCGGGGGTTA TGAGCCTTTA TTCACGATGA GTGCACAGCA ACAGTCCATC 
CAGCCATTCA CCCCACAGCA ATATGAAGCG CTTACCTATG AGTTTGAGAG ACAGCCGACT 
GCCATGCAGT TCATTACTTT GTATAGTTAT GAGGACATAG TGACTGCGCG AATTGAGGGA 
AGTTCAGGAT CTGTATGGGG AATCAGCCCT CCAAGCCGTG AGCAGATGAG AAAGGAGCTG 
CAAAATGGAT CCTCTGACAT TACACTACGC TTCACCTGGG ATTTTCAGAG GGATCTGGCA 
AAAGGTGGCA CTGTGGAGCA CACATCTGAG AAACACACTA AGGACTTGGA GCCTGGGAGC 
CAGGAGAGGA TGGAGCTGGC AGATCTTCTT CAAGGAGTAC GAGACACCCC TGTCTCTATC 
CAGCACTTGT TTCCAAAGTA CATCCGCGCT CCTAATGGCC CAGAAGCAAA TCCAGTGAAA 
CAGCTCCAGC CAGATGAAGA AGAAAGTTAC CTGGATGTGG AGGTGCAGCT AAATCGGATG 
CGAGTTGCAG AGGGAAATAG CACCAGCAGT TTTGTGGAGT GGTGGGTTAT TACTCTAAAA 
GACTGCGAGG GAGAAGACTG CAACATACTA CCTATGGTGA TTTTTAACGA TAAAGTTAGC 
CCTCCTAGCT TAGGATTCCT GGCTGGATAT GGGATAATGG GTTTGTATGT ATCTATTGTC 
CTGGTCATTG GGAAGTTTGT GCGCGGCTTC TTCAGTGAAA TCTCTCACTC CATCATGTTT 
GAGGAGCTGC CTTGTGTGGA TCGGATTCTC AAGCTGTGCC AGGATATCTA TGTGGTGCGG 
GAGCGTGGGG AATTGGAACT GGAAGAGGAA CTCTATGCAA AACTCATCTT CCTGTATCGC 
TCACCAGAAA CCATGATCAA GTGGACTGTA GATAAGGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817734.2
CDS	820..8241
Translation	MELQVLCGVLFWLLLPGSLLAACLFRYNSLSLVYLFYLLLLPWFAGPNPCSMRGHTGRLMKALLATSILFLCAHLIFQICLYTIPELDDLLGHNCSSWDVLARHVGINRLDLSDIPNMVRQVSPDFGMFVVSALCLGLCNRLVTKKRRQRSSGYFEQEEERTELNLKYESRECDTEFEVGGDLHSVPRDRLLGSWLRKAAYRLLETVEKSLAVLLLALAGITDPSAFSAPYYVVFIGLCTWWACHFPISQLGFNTICVIVGFFTGGHMICLYIYQTPFIQQMFHPEQLWARLFGLHNIISHSNITDCTHDSLNQNLDWPVYVHPGILLLLYYSLLSILKLKSSDKKRISGCDLRKSQQEDMELTDIEPVSKEQYSAGEETKPMLQRMNTEEEAKPQDCTVHVIRRNSNQLPELETKEPSQFHLMIHVILSQSYICALIAMMVWSITYHSWLTFVLLLWACLIWMVRNRRQFAMLCSPFILMYGVTLCSLQYVWSMDLREELPTQLGFMKLSQLGLVRAPYPCLNMGALLLYTLTFWLLLHQFVTEKFLKRKKFMSPLLEVTVADAGKEKNLLKRFGEQVKSFYAKYWIYVCGGMFIVVSFAGRLVVYKIVYMLLFLLCLVLFQVYYSLWRKLLTFFWWLVVAYTMLVLITIYTFQFEEFPEYWKNLTNLTDEQLGDIGLERFSVSELFTSILIPGFFLLACILQLHYFHRPFMHMTNLAHASCQGSFHSHESEQPKEEEEKAGACSDAAADEEEDEEESAHGADLSKWDLVTQRLSVLSFKFSELLHRLQVFLWRLLELHVLKLVALYSVWVALQEVSVMNFLFVVLWAFAMPFSRFQHMASCLCTIWTSIIIVCKMLYQLTVVNPLEYSSNCTQPPLNNTSLLEDEILNSTLYREPIDPANWFGIRKGYPNLGYIQNHLQILLLLVFEAIVYRHQEFYRKQYQLEKPHIQAVFPDVYREHLDQGLFSCIKYFINYSFYKFGLEICFLLTVNVIGQRMNFMVIFHGCWLIVILTRRQRVNIAKLWPKYCVFLIFFLLWQYMICLGIPPVLCIDYPWRWDTFVPVNSALIKWLYLPDFYTTPKSVNLISDFVLLIGVAQQWKVFINEKKEEWYTMAGENVEEYEAPKDRPNPVPNFINCQSYLDMVKVAVFRYLFWFVLTVVFITGATRISVFGLGYLMSCFYLLLSGTTLLQKPMKAKLVLWDCLILYNITVILSKNMLSLLSCVFVQQMQTNFCWVIQLFSLVCTVKGYYNPQDMDKDRDCSLPVEEAGIIWDSICFLFLLLQRRVFLSCYFRHVVLDLRASSLQTSRGFELFMASIRKNVFFHQESERRSLNQLRKQMERIRAKQERYHEGRRQTDSSQVKEGEPGSKKGSSRQKIHWSRPWLDHASVLHSGDYFLFESDSEEDEESLLEQDQRPPKQSAFQLAYQAWVTSAKSALKERKSRQKERSGKVEMVTVENNEAGGGTESHTQEPREKGEDKDLNEERGGSNVVQRILDVLKFLWVLLLAMVDGLINWLGTLTKQHVDMSTVLWVERYIITQKLSKGEDINRRDLEKVYETGQDVREPRISSPPTDMSTSIASSGCEEEELICSTAECTENITQLSTGYNTRSNSLDSIRGFPEIKPSHRSRTASELLLSRPLDFPELSESQHFFQSQSRILRLLLALYNFVAAHSELLCYFIIILNNMVSASLISLFLPILIFLWGMLSIHKPSKRFWMTAIIFTEVMVVIKYLFQFGFFPWNNYTILVRNEGKPFFPPRVLGLEKSDSYIKYDLIQLLMLFFHRSLLMSYGLWDRKDLDLEERTPGKDKGSKTEIQASQDLEPAGGSSSSLRQRKRKESQGSVQERDSLKEKSLDVQKKKKGRVALRVKIMGMKMQQFFACIVRNIYQPVKAFFHDILHTKNRAATDVYAFMFLADVVDFVIIVFGFWAFGKHSAAADIASSLSDDQVPEAFLVMVLIQFSTMVIDRALYLRKTVLGKLIFQVILVIGIHVWMFFILPAVTERMFNQNTVAQLWYFVKCIYFALSAYQIRCGYPTRILGNFLTKKYNHLNLFLFQGFRLVPFLVELRAVMDWVWTDTTLSLSNWMCVEDIYANIFIIKCSRETEKKYPQPKGQKKKKIVKYGMGGLIIVFLICIIWFPLLFMSLVRSVVGVVNHPIDVTVTFKLGGYEPLFTMSAQQQSIQPFTPQQYEALTYEFERQPTAMQFITLYSYEDIVTARIEGSSGSVWGISPPSREQMRKELQNGSSDITLRFTWDFQRDLAKGGTVEHTSEKHTKDLEPGSQERMELADLLQGVRDTPVSIQHLFPKYIRAPNGPEANPVKQLQPDEEESYLDVEVQLNRMRVAEGNSTSSFVEWWVITLKDCEGEDCNILPMVIFNDKVSPPSLGFLAGYGIMGLYVSIVLVIGKFVRGFFSEISHSIMFEELPCVDRILKLCQDIYVVRERGELELEEELYAKLIFLYRSPETMIKWTVDKD

Target ORF information:

RefSeq Version	XM_012962280.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis piezo-type mechanosensitive ion channel component 1 (piezo1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012962280.2

ORF Insert Sequence:

ATGGAACTGC AAGTCCTCTG TGGGGTCCTG TTTTGGCTCC TTCTGCCCGG CTCCCTCTTG 
GCAGCCTGCT TATTCCGTTA CAACTCCTTG TCACTGGTGT ACCTGTTTTA TCTTCTGCTT 
CTGCCATGGT TTGCTGGACC AAATCCCTGC AGCATGAGAG GTCACACAGG ACGTCTGATG 
AAAGCGTTAC TTGCTACCAG CATCCTCTTC CTCTGTGCTC ATCTCATCTT CCAGATCTGC 
CTGTACACAA TACCTGAACT GGATGACCTG CTAGGGCACA ACTGCAGCAG CTGGGATGTG 
TTGGCTCGTC ACGTAGGTAT AAACAGGCTG GATCTTTCTG ATATCCCCAA CATGGTGCGA 
CAAGTGTCCC CAGATTTTGG CATGTTTGTC GTGTCAGCAT TGTGCCTTGG CTTGTGTAAT 
CGCCTAGTGA CCAAGAAGAG ACGACAGAGG TCTTCAGGCT ATTTTGAGCA GGAAGAAGAG 
AGGACTGAAC TTAACCTAAA ATATGAAAGT AGAGAGTGTG ATACAGAGTT TGAGGTGGGG 
GGAGACTTAC ATTCTGTTCC ACGAGACCGC CTTCTTGGGT CCTGGCTTAG AAAGGCAGCA 
TACAGGCTTC TGGAAACAGT GGAGAAATCA CTTGCTGTCT TACTCTTGGC TCTTGCAGGT 
ATCACTGATC CCTCTGCTTT CTCAGCCCCA TATTATGTGG TTTTCATTGG TCTCTGCACA 
TGGTGGGCCT GCCATTTTCC CATTAGCCAA CTAGGCTTTA ATACTATCTG TGTCATTGTT 
GGGTTCTTCA CTGGAGGTCA CATGATTTGC CTTTATATTT ACCAGACACC TTTTATCCAA 
CAAATGTTTC ATCCTGAACA GTTATGGGCA AGGTTGTTTG GTCTGCATAA CATCATCAGT 
CACTCCAACA TTACAGACTG TACCCATGAC TCTCTGAATC AAAACCTTGA CTGGCCTGTG 
TATGTTCATC CCGGAATCTT ATTACTGCTG TACTACTCCC TATTATCAAT ACTGAAACTG 
AAGAGCTCTG ACAAGAAAAG GATTTCAGGG TGTGACCTTC GGAAATCTCA GCAGGAGGAT 
ATGGAGTTGA CTGATATTGA GCCAGTTTCA AAGGAGCAAT ATAGTGCGGG AGAAGAGACA 
AAACCAATGC TTCAGAGGAT GAACACTGAA GAAGAGGCTA AACCCCAAGA CTGCACTGTT 
CATGTGATTA GAAGAAATTC TAATCAACTG CCAGAACTTG AAACCAAGGA GCCATCACAA 
TTTCACCTGA TGATTCATGT AATACTGAGC CAAAGCTATA TCTGTGCGCT TATTGCAATG 
ATGGTGTGGA GCATTACATA TCACAGCTGG CTGACTTTTG TGCTGCTGCT CTGGGCATGT 
TTAATCTGGA TGGTGAGGAA CAGGCGCCAG TTTGCAATGC TGTGCTCTCC CTTTATTTTG 
ATGTATGGAG TGACTCTCTG CAGCCTGCAG TATGTCTGGA GTATGGACTT GAGAGAAGAG 
CTTCCGACAC AACTTGGTTT TATGAAGCTG TCACAGCTGG GACTGGTTCG CGCACCATAC 
CCCTGCCTTA ACATGGGAGC ACTGCTACTT TACACGCTGA CATTTTGGCT TCTGCTGCAC 
CAATTTGTAA CAGAAAAATT TCTAAAACGT AAGAAGTTTA TGTCCCCACT CCTGGAGGTC 
ACAGTGGCAG ATGCTGGCAA AGAAAAGAAC CTGCTAAAAC GTTTTGGGGA GCAAGTAAAA 
AGCTTCTATG CAAAATACTG GATCTATGTG TGTGGAGGCA TGTTTATTGT GGTCAGCTTT 
GCTGGTAGGC TGGTAGTTTA CAAAATAGTC TACATGCTTC TGTTCCTACT ATGCTTGGTG 
TTATTTCAGG TGTATTACTC TTTGTGGAGG AAGCTGCTGA CTTTTTTCTG GTGGTTAGTT 
GTAGCATACA CAATGTTGGT TCTTATCACC ATCTACACCT TTCAATTTGA AGAGTTTCCT 
GAGTACTGGA AAAACTTGAC CAACCTTACA GATGAGCAGT TGGGTGATAT TGGGCTTGAG 
AGGTTTAGTG TGTCGGAACT TTTTACCAGC ATCCTTATCC CAGGCTTCTT CCTCCTTGCC 
TGCATCCTCC AGTTACACTA CTTTCATCGC CCTTTTATGC ACATGACCAA CTTGGCACAT 
GCCTCATGCC AAGGAAGTTT CCATAGCCAC GAGAGTGAGC AGCCAAAGGA AGAAGAAGAG 
AAAGCTGGTG CATGCAGTGA TGCTGCTGCT GATGAGGAGG AGGATGAGGA AGAATCTGCA 
CATGGAGCTG ATCTAAGTAA ATGGGACCTA GTTACACAGC GCCTTTCTGT CCTGTCCTTT 
AAGTTCTCTG AGCTGCTGCA CCGATTACAG GTTTTCCTTT GGAGGCTTTT GGAGCTCCAT 
GTGTTGAAAC TGGTGGCTTT GTATAGCGTG TGGGTTGCCC TGCAGGAGGT ATCTGTGATG 
AATTTCCTTT TTGTGGTCCT ATGGGCTTTT GCTATGCCTT TCTCCCGATT CCAGCACATG 
GCTTCCTGTC TTTGCACCAT CTGGACCTCT ATAATTATTG TTTGCAAAAT GCTTTACCAG 
CTTACTGTTG TCAACCCTCT GGAGTACTCT AGCAACTGTA CTCAGCCTCC TCTAAATAAC 
ACCAGTCTCC TAGAAGACGA GATTTTGAAC TCCACCCTTT ATAGAGAACC TATAGATCCA 
GCAAACTGGT TTGGTATCAG AAAAGGTTAT CCAAACCTAG GTTACATACA GAATCACCTG 
CAAATACTCC TACTTTTGGT ATTTGAGGCA ATTGTTTATC GGCATCAGGA GTTTTACCGT 
AAACAATACC AGCTTGAGAA GCCACACATT CAGGCTGTAT TTCCAGATGT CTACAGAGAG 
CACTTGGACC AAGGCTTGTT CAGCTGTATC AAATATTTTA TCAACTATTC CTTTTATAAG 
TTTGGTCTTG AGATCTGCTT CCTTCTTACT GTGAATGTGA TTGGTCAGAG GATGAACTTT 
ATGGTAATCT TTCATGGCTG CTGGCTGATA GTGATTCTTA CTCGACGACA GCGAGTCAAC 
ATTGCAAAAT TATGGCCTAA ATACTGTGTG TTCCTAATCT TTTTCCTGTT GTGGCAGTAC 
ATGATATGTC TGGGCATTCC TCCGGTGCTT TGTATAGATT ATCCCTGGAG GTGGGACACA 
TTTGTTCCTG TCAACTCTGC ACTGATAAAG TGGTTATATC TTCCAGATTT CTATACTACA 
CCTAAATCTG TCAACCTTAT CAGTGATTTT GTTTTGCTGA TTGGTGTGGC CCAGCAGTGG 
AAGGTGTTTA TTAATGAGAA GAAGGAAGAA TGGTACACTA TGGCAGGGGA AAATGTGGAA 
GAGTATGAAG CTCCAAAAGA CAGGCCCAAC CCTGTACCTA ACTTCATCAA TTGTCAGTCT 
TATCTGGATA TGGTGAAAGT AGCAGTGTTT CGGTACCTAT TCTGGTTTGT CTTGACTGTG 
GTCTTTATAA CAGGGGCCAC TCGCATCAGT GTTTTTGGCC TAGGATATTT GATGTCCTGT 
TTCTACCTTC TTCTGTCTGG CACAACCCTT CTGCAAAAGC CAATGAAAGC CAAGCTGGTG 
CTTTGGGACT GTTTGATCCT CTACAACATC ACTGTTATAC TGTCCAAGAA CATGCTGTCG 
CTCCTGTCTT GTGTATTTGT TCAACAAATG CAAACCAACT TTTGCTGGGT GATCCAGCTC 
TTCAGCCTAG TTTGCACAGT TAAGGGTTAC TACAATCCCC AAGATATGGA TAAAGACCGG 
GATTGCTCTC TGCCAGTGGA AGAGGCCGGA ATCATTTGGG ATAGCATCTG CTTCTTATTT 
CTCCTTCTCC AACGAAGAGT TTTTTTGAGT TGCTACTTTC GGCATGTTGT TCTTGACCTT 
CGTGCCTCCT CCCTTCAAAC ATCAAGAGGT TTTGAGCTCT TCATGGCCAG CATCAGAAAG 
AATGTGTTTT TCCACCAAGA ATCTGAGAGA CGATCTCTTA ACCAGCTCAG GAAGCAAATG 
GAGAGAATCC GAGCAAAGCA AGAACGCTAT CATGAAGGCA GAAGACAAAC TGATAGCAGT 
CAAGTAAAGG AAGGTGAGCC TGGCAGCAAG AAAGGGTCCA GTAGGCAAAA GATTCATTGG 
AGCCGCCCAT GGTTAGACCA CGCCTCAGTG CTGCATTCAG GAGATTATTT TCTGTTCGAA 
TCAGACAGTG AAGAGGATGA AGAGTCTTTA TTGGAGCAGG ATCAGAGGCC ACCCAAGCAG 
AGTGCTTTTC AGCTTGCATA CCAGGCATGG GTTACAAGTG CCAAATCTGC GCTAAAGGAA 
AGAAAGAGTC GGCAGAAAGA GAGATCTGGA AAGGTAGAAA TGGTCACGGT AGAGAATAAT 
GAAGCAGGAG GGGGGACAGA GTCTCATACC CAGGAACCAA GGGAGAAAGG AGAGGATAAA 
GATCTGAATG AGGAGAGAGG AGGAAGCAAT GTGGTACAGC GTATCCTTGA CGTGTTGAAG 
TTTCTCTGGG TTCTTTTGCT TGCCATGGTG GATGGACTGA TCAATTGGCT AGGAACCCTT 
ACAAAGCAGC ATGTTGATAT GTCCACAGTA CTGTGGGTAG AGAGATATAT CATAACCCAA 
AAACTGTCTA AGGGAGAAGA TATCAACCGC AGGGATTTGG AAAAGGTATA TGAGACAGGG 
CAGGATGTTA GGGAGCCTAG GATTTCATCT CCTCCAACTG ATATGTCAAC TAGCATTGCT 
TCAAGTGGCT GTGAGGAAGA GGAGCTTATC TGCAGCACAG CAGAGTGTAC GGAAAATATA 
ACACAACTCA GCACCGGGTA CAACACACGA AGTAACAGCT TGGACTCTAT TAGGGGGTTT 
CCGGAAATCA AGCCAAGTCA TCGTTCTCGT ACTGCTAGTG AATTGCTGCT TTCCAGGCCA 
CTTGATTTCC CAGAGCTCTC CGAGTCTCAG CACTTCTTTC AGTCTCAGAG TCGTATTCTG 
AGGCTCCTGT TGGCCCTCTA TAACTTTGTG GCTGCTCACT CTGAGCTGCT TTGTTACTTC 
ATCATCATTC TCAACAACAT GGTGAGCGCT TCCCTGATAT CACTGTTCCT TCCAATTCTC 
ATCTTCCTGT GGGGTATGCT GTCCATCCAC AAACCCAGTA AGCGCTTCTG GATGACTGCT 
ATCATTTTTA CTGAGGTTAT GGTAGTAATA AAATACCTCT TCCAGTTTGG TTTCTTCCCA 
TGGAATAATT ATACCATTCT GGTACGCAAC GAAGGGAAAC CATTTTTTCC TCCACGTGTC 
CTGGGACTTG AGAAGAGTGA CTCGTACATC AAATATGATC TGATCCAGCT CTTGATGCTC 
TTTTTCCATC GCTCACTGCT CATGAGTTAT GGTCTTTGGG ATCGTAAAGA CTTAGACCTT 
GAAGAGAGAA CACCCGGGAA GGATAAAGGG AGCAAAACAG AGATTCAGGC ATCCCAGGAT 
CTGGAGCCAG CAGGAGGCAG TAGCTCTTCC CTGCGTCAGC GCAAAAGAAA AGAATCCCAG 
GGCAGTGTGC AAGAAAGAGA TTCCTTAAAA GAGAAATCAC TAGATGTGCA GAAGAAGAAA 
AAAGGAAGAG TGGCTCTTAG GGTGAAGATC ATGGGAATGA AAATGCAACA GTTCTTTGCC 
TGCATAGTGC GGAATATATA TCAGCCAGTG AAAGCTTTTT TCCATGATAT TCTACATACC 
AAGAACCGTG CTGCAACAGA TGTTTATGCC TTCATGTTCC TCGCAGATGT GGTTGACTTT 
GTGATCATCG TATTTGGGTT TTGGGCTTTT GGAAAACACT CTGCTGCTGC CGACATAGCA 
TCATCTCTCT CTGATGACCA AGTACCAGAA GCTTTCCTAG TCATGGTCCT CATACAGTTT 
TCCACCATGG TGATAGATCG TGCTCTATAC CTGCGGAAGA CTGTGCTTGG AAAGCTCATC 
TTTCAAGTTA TTCTGGTGAT TGGGATACAT GTCTGGATGT TCTTTATCCT ACCAGCAGTC 
ACAGAAAGGA TGTTCAATCA GAACACTGTG GCCCAGCTCT GGTATTTTGT TAAGTGCATT 
TACTTTGCCC TGTCTGCCTA CCAGATCCGC TGTGGGTATC CTACCCGCAT CCTGGGCAAC 
TTCCTTACCA AGAAATACAA CCACCTCAAC CTATTCCTAT TTCAGGGGTT TCGCTTGGTT 
CCGTTTCTAG TAGAGCTCAG GGCTGTAATG GACTGGGTTT GGACTGATAC CACCTTGTCC 
TTGTCCAACT GGATGTGCGT GGAAGACATT TATGCTAATA TCTTCATTAT CAAATGCAGC 
CGAGAGACTG AAAAGAAATA CCCTCAGCCT AAAGGACAGA AAAAAAAGAA GATAGTGAAA 
TATGGAATGG GTGGTTTGAT CATAGTCTTC CTTATCTGTA TAATCTGGTT TCCCCTTCTC 
TTCATGTCGT TGGTGCGCTC AGTGGTGGGA GTCGTCAACC ACCCTATCGA TGTCACGGTT 
ACTTTTAAAC TCGGGGGTTA TGAGCCTTTA TTCACGATGA GTGCACAGCA ACAGTCCATC 
CAGCCATTCA CCCCACAGCA ATATGAAGCG CTTACCTATG AGTTTGAGAG ACAGCCGACT 
GCCATGCAGT TCATTACTTT GTATAGTTAT GAGGACATAG TGACTGCGCG AATTGAGGGA 
AGTTCAGGAT CTGTATGGGG AATCAGCCCT CCAAGCCGTG AGCAGATGAG AAAGGAGCTG 
CAAAATGGAT CCTCTGACAT TACACTACGC TTCACCTGGG ATTTTCAGAG GGATCTGGCA 
AAAGGTGGCA CTGTGGAGCA CACATCTGAG AAACACACTA AGGACTTGGA GCCTGGGAGC 
CAGGAGAGGA TGGAGCTGGC AGATCTTCTT CAAGGAGTAC GAGACACCCC TGTCTCTATC 
CAGCACTTGT TTCCAAAGTA CATCCGCGCT CCTAATGGCC CAGAAGCAAA TCCAGTGAAA 
CAGCTCCAGC CAGATGAAGA AGAAAGTTAC CTGGATGTGG AGGTGCAGCT AAATCGGATG 
CGAGTTGCAG AGGGAAATAG CACCAGCAGT TTTGTGGAGT GGTGGGTTAT TACTCTAAAA 
GACTGCGAGG GAGAAGACTG CAACATACTA CCTATGGTGA TTTTTAACGA TAAAGTTAGC 
CCTCCTAGCT TAGGATTCCT GGCTGGATAT GGGATAATGG GTTTGTATGT ATCTATTGTC 
CTGGTCATTG GGAAGTTTGT GCGCGGCTTC TTCAGTGAAA TCTCTCACTC CATCATGTTT 
GAGGAGCTGC CTTGTGTGGA TCGGATTCTC AAGCTGTGCC AGGATATCTA TGTGGTGCGG 
GAGCGTGGGG AATTGGAACT GGAAGAGGAA CTCTATGCAA AACTCATCTT CCTGTATCGC 
TCACCAGAAA CCATGATCAA GTGGACTGTA GATAAGGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

piezo1 ( XM_012962282.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817736.2 Xenopus tropicalis piezo-type mechanosensitive ion channel component 1 (piezo1), transcript variant X3, mRNA.

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CloneID	OXa56852
Clone ID Related Accession (Same CDS sequence)	XM_012962282.3
Accession Version	XM_012962282.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	piezo-type mechanosensitive ion channel component 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012962282.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACTGC AAGTCCTCTG TGGGGTCCTG TTTTGGCTCC TTCTGCCCGG CTCCCTCTTG 
GCAGCCTGCT TATTCCGTTA CAACTCCTTG TCACTGGTGT ACCTGTTTTA TCTTCTGCTT 
CTGCCATGGT TTGCTGGACC AAATCCCTGC AGCATGAGAG GTCACACAGG ACGTCTGATG 
AAAGCGTTAC TTGCTACCAG CATCCTCTTC CTCTGTGCTC ATCTCATCTT CCAGATCTGC 
CTGTACACAA TACCTGAACT GGATGACCTG CTAGGGCACA ACTGCAGCAG CTGGGATGTG 
TTGGCTCGTC ACGTAGGTAT AAACAGGCTG GATCTTTCTG ATATCCCCAA CATGGTGCGA 
CAAGTGTCCC CAGATTTTGG CATGTTTGTC GTGTCAGCAT TGTGCCTTGG CTTGTGTAAT 
CGCCTAGTGA CCAAGAAGAG ACGACAGAGG TCTTCAGGCT ATTTTGAGCA GGAAGAAGAG 
AGGACTGAAC TTAACCTAAA ATATGAAAGT AGAGAGTGTG ATACAGAGTT TGAGGTGGGG 
GGAGACTTAC ATTCTGTTCC ACGAGACCGC CTTCTTGGGT CCTGGCTTAG AAAGGCAGCA 
TACAGGCTTC TGGAAACAGT GGAGAAATCA CTTGCTGTCT TACTCTTGGC TCTTGCAGGT 
ATCACTGATC CCTCTGCTTT CTCAGCCCCA TATTATGTGG TTTTCATTGG TCTCTGCACA 
TGGTGGGCCT GCCATTTTCC CATTAGCCAA CTAGGCTTTA ATACTATCTG TGTCATTGTT 
GGGTTCTTCA CTGGAGGTCA CATGATTTGC CTTTATATTT ACCAGACACC TTTTATCCAA 
CAAATGTTTC ATCCTGAACA GTTATGGGCA AGGTTGTTTG GTCTGCATAA CATCATCAGT 
CACTCCAACA TTACAGACTG TACCCATGAC TCTCTGAATC AAAACCTTGA CTGGCCTGTG 
TATGTTCATC CCGGAATCTT ATTACTGCTG TACTACTCCC TATTATCAAT ACTGAAACTG 
AAGAGCTCTG ACAAGAAAAG GATTTCAGGG TGTGACCTTC GGAAATCTCA GCAGGAGGAT 
ATGGAGTTGA CTGATATTGA GCCAGTTTCA AAGGAGCAAT ATAGTGCGGG AGAAGAGACA 
AAACCAATGC TTCAGAGGAT GAACACTGAA GAAGAGGCTA AACCCCAAGA CTGCACTGTT 
CATGTGATTA GAAGAAATTC TAATCAACTG CCAGAAGAAC TTGAAACCAA GGAGCCATCA 
CAATTTCACC TGATGATTCA TGTAATACTG AGCCAAAGCT ATATCTGTGC GCTTATTGCA 
ATGATGGTGT GGAGCATTAC ATATCACAGC TGGCTGACTT TTGTGCTGCT GCTCTGGGCA 
TGTTTAATCT GGATGGTGAG GAACAGGCGC CAGTTTGCAA TGCTGTGCTC TCCCTTTATT 
TTGATGTATG GAGTGACTCT CTGCAGCCTG CAGTATGTCT GGAGTATGGA CTTGAGAGAA 
GAGCTTCCGA CACAACTTGG TTTTATGAAG CTGTCACAGC TGGGACTGGT TCGCGCACCA 
TACCCCTGCC TTAACATGGG AGCACTGCTA CTTTACACGC TGACATTTTG GCTTCTGCTG 
CACCAATTTG TAACAGAAAA ATTTCTAAAA CGTAAGAAGT TTATGTCCCC ACTCCTGGAG 
GTCACAGTGG CAGATGCTGG CAAAGAAAAG AACCTGCTAA AACGTTTTGG GGAGCAAGTA 
AAAAGCTTCT ATGCAAAATA CTGGATCTAT GTGTGTGGAG GCATGTTTAT TGTGGTCAGC 
TTTGCTGGTA GGCTGGTAGT TTACAAAATA GTCTACATGC TTCTGTTCCT ACTATGCTTG 
GTGTTATTTC AGGTGTATTA CTCTTTGTGG AGGAAGCTGC TGACTTTTTT CTGGTGGTTA 
GTTGTAGCAT ACACAATGTT GGTTCTTATC ACCATCTACA CCTTTCAATT TGAAGAGTTT 
CCTGAGTACT GGAAAAACTT GACCAACCTT ACAGATGAGC AGTTGGGTGA TATTGGGCTT 
GAGAGGTTTA GTGTGTCGGA ACTTTTTACC AGCATCCTTA TCCCAGGCTT CTTCCTCCTT 
GCCTGCATCC TCCAGTTACA CTACTTTCAT CGCCCTTTTA TGCACATGAC CAACTTGGCA 
CATGCCTCAT GCCAAGGAAG TTTCCATAGC CACGAGAGTG AGCAGCCAAA GGAAGAAGAA 
GAGAAAGCTG GTGCATGCAG TGATGCTGCT GCTGATGAGG AGGAGGATGA GGAAGAATCT 
GCACATGGAG CTGATCTAAG TAAATGGGAC CTAGTTACAC AGCGCCTTTC TGTCCTGTCC 
TTTAAGTTCT CTGAGCTGCT GCACCGATTA CAGGTTTTCC TTTGGAGGCT TTTGGAGCTC 
CATGTGTTGA AACTGGTGGC TTTGTATAGC GTGTGGGTTG CCCTGCAGGA GGTATCTGTG 
ATGAATTTCC TTTTTGTGGT CCTATGGGCT TTTGCTATGC CTTTCTCCCG ATTCCAGCAC 
ATGGCTTCCT GTCTTTGCAC CATCTGGACC TCTATAATTA TTGTTTGCAA AATGCTTTAC 
CAGCTTACTG TTGTCAACCC TCTGGAGTAC TCTAGCAACT GTACTCAGCC TCCTCTAAAT 
AACACCAGTC TCCTAGAAGA CGAGATTTTG AACTCCACCC TTTATAGAGA ACCTATAGAT 
CCAGCAAACT GGTTTGGTAT CAGAAAAGGT TATCCAAACC TAGGTTACAT ACAGAATCAC 
CTGCAAATAC TCCTACTTTT GGTATTTGAG GCAATTGTTT ATCGGCATCA GGAGTTTTAC 
CGTAAACAAT ACCAGCTTGA GAAGCCACAC ATTCAGGCTG TATTTCCAGA TGTCTACAGA 
GAGCACTTGG ACCAAGGCTT GTTCAGCTGT ATCAAATATT TTATCAACTA TTCCTTTTAT 
AAGTTTGGTC TTGAGATCTG CTTCCTTCTT ACTGTGAATG TGATTGGTCA GAGGATGAAC 
TTTATGGTAA TCTTTCATGG CTGCTGGCTG ATAGTGATTC TTACTCGACG ACAGCGAGTC 
AACATTGCAA AATTATGGCC TAAATACTGT GTGTTCCTAA TCTTTTTCCT GTTGTGGCAG 
TACATGATAT GTCTGGGCAT TCCTCCGGTG CTTTGTATAG ATTATCCCTG GAGGTGGGAC 
ACATTTGTTC CTGTCAACTC TGCACTGATA AAGTGGTTAT ATCTTCCAGA TTTCTATACT 
ACACCTAAAT CTGTCAACCT TATCAGTGAT TTTGTTTTGC TGATTGGTGT GGCCCAGCAG 
TGGAAGGTGT TTATTAATGA GAAGAAGGAA GAATGGTACA CTATGGCAGG GGAAAATGTG 
GAAGAGTATG AAGCTCCAAA AGACAGGCCC AACCCTGTAC CTAACTTCAT CAATTGTCAG 
TCTTATCTGG ATATGGTGAA AGTAGCAGTG TTTCGGTACC TATTCTGGTT TGTCTTGACT 
GTGGTCTTTA TAACAGGGGC CACTCGCATC AGTGTTTTTG GCCTAGGATA TTTGATGTCC 
TGTTTCTACC TTCTTCTGTC TGGCACAACC CTTCTGCAAA AGCCAATGAA AGCCAAGCTG 
GTGCTTTGGG ACTGTTTGAT CCTCTACAAC ATCACTGTTA TACTGTCCAA GAACATGCTG 
TCGCTCCTGT CTTGTGTATT TGTTCAACAA ATGCAAACCA ACTTTTGCTG GGTGATCCAG 
CTCTTCAGCC TAGTTTGCAC AGTTAAGGGT TACTACAATC CCCAAGATAT GGATAAAGAC 
CGGGATTGCT CTCTGCCAGT GGAAGAGGCC GGAATCATTT GGGATAGCAT CTGCTTCTTA 
TTTCTCCTTC TCCAACGAAG AGTTTTTTTG AGTTGCTACT TTCGGCATGT TGTTCTTGAC 
CTTCGTGCCT CCTCCCTTCA AACATCAAGA GGTTTTGAGC TCTTCATGGC CAGCATCAGA 
AAGAATGTGT TTTTCCACCA AGAATCTGAG AGACGATCTC TTAACCAGCT CAGGAAGCAA 
ATGGAGAGAA TCCGAGCAAA GCAAGAACGC TATCATGAAG GCAGAAGACA AACTGATAGC 
AGTCAAGTAA AGGAAGTGCT GCATTCAGGA GATTATTTTC TGTTCGAATC AGACAGTGAA 
GAGGATGAAG AGTCTTTATT GGAGCAGGAT CAGAGGCCAC CCAAGCAGAG TGCTTTTCAG 
CTTGCATACC AGGCATGGGT TACAAGTGCC AAATCTGCGC TAAAGGAAAG AAAGAGTCGG 
CAGAAAGAGA GATCTGGAAA GGTAGAAATG GTCACGGTAG AGAATAATGA AGCAGGAGGG 
GGGACAGAGT CTCATACCCA GGAACCAAGG GAGAAAGGAG AGGATAAAGA TCTGAATGAG 
GAGAGAGGAG GAAGCAATGT GGTACAGCGT ATCCTTGACG TGTTGAAGTT TCTCTGGGTT 
CTTTTGCTTG CCATGGTGGA TGGACTGATC AATTGGCTAG GAACCCTTAC AAAGCAGCAT 
GTTGATATGT CCACAGTACT GTGGGTAGAG AGATATATCA TAACCCAAAA ACTGTCTAAG 
GGAGAAGATA TCAACCGCAG GGATTTGGAA AAGGTATATG AGACAGGGCA GGATGTTAGG 
GAGCCTAGGA TTTCATCTCC TCCAACTGAT ATGTCAACTA GCATTGCTTC AAGTGGCTGT 
GAGGAAGAGG AGCTTATCTG CAGCACAGCA GAGTGTACGG AAAATATAAC ACAACTCAGC 
ACCGGGTACA ACACACGAAG TAACAGCTTG GACTCTATTA GGGGGTTTCC GGAAATCAAG 
CCAAGTCATC GTTCTCGTAC TGCTAGTGAA TTGCTGCTTT CCAGGCCACT TGATTTCCCA 
GAGCTCTCCG AGTCTCAGCA CTTCTTTCAG TCTCAGAGTC GTATTCTGAG GCTCCTGTTG 
GCCCTCTATA ACTTTGTGGC TGCTCACTCT GAGCTGCTTT GTTACTTCAT CATCATTCTC 
AACAACATGG TGAGCGCTTC CCTGATATCA CTGTTCCTTC CAATTCTCAT CTTCCTGTGG 
GGTATGCTGT CCATCCACAA ACCCAGTAAG CGCTTCTGGA TGACTGCTAT CATTTTTACT 
GAGGTTATGG TAGTAATAAA ATACCTCTTC CAGTTTGGTT TCTTCCCATG GAATAATTAT 
ACCATTCTGG TACGCAACGA AGGGAAACCA TTTTTTCCTC CACGTGTCCT GGGACTTGAG 
AAGAGTGACT CGTACATCAA ATATGATCTG ATCCAGCTCT TGATGCTCTT TTTCCATCGC 
TCACTGCTCA TGAGTTATGG TCTTTGGGAT CGTAAAGACT TAGACCTTGA AGAGAGAACA 
CCCGGGAAGG ATAAAGGGAG CAAAACAGAG ATTCAGGCAT CCCAGGATCT GGAGCCAGCA 
GGAGGCAGTA GCTCTTCCCT GCGTCAGCGC AAAAGAAAAG AATCCCAGGG CAGTGTGCAA 
GAAAGAGATT CCTTAAAAGA GAAATCACTA GATGTGCAGA AGAAGAAAAA AGGAAGAGTG 
GCTCTTAGGG TGAAGATCAT GGGAATGAAA ATGCAACAGT TCTTTGCCTG CATAGTGCGG 
AATATATATC AGCCAGTGAA AGCTTTTTTC CATGATATTC TACATACCAA GAACCGTGCT 
GCAACAGATG TTTATGCCTT CATGTTCCTC GCAGATGTGG TTGACTTTGT GATCATCGTA 
TTTGGGTTTT GGGCTTTTGG AAAACACTCT GCTGCTGCCG ACATAGCATC ATCTCTCTCT 
GATGACCAAG TACCAGAAGC TTTCCTAGTC ATGGTCCTCA TACAGTTTTC CACCATGGTG 
ATAGATCGTG CTCTATACCT GCGGAAGACT GTGCTTGGAA AGCTCATCTT TCAAGTTATT 
CTGGTGATTG GGATACATGT CTGGATGTTC TTTATCCTAC CAGCAGTCAC AGAAAGGATG 
TTCAATCAGA ACACTGTGGC CCAGCTCTGG TATTTTGTTA AGTGCATTTA CTTTGCCCTG 
TCTGCCTACC AGATCCGCTG TGGGTATCCT ACCCGCATCC TGGGCAACTT CCTTACCAAG 
AAATACAACC ACCTCAACCT ATTCCTATTT CAGGGGTTTC GCTTGGTTCC GTTTCTAGTA 
GAGCTCAGGG CTGTAATGGA CTGGGTTTGG ACTGATACCA CCTTGTCCTT GTCCAACTGG 
ATGTGCGTGG AAGACATTTA TGCTAATATC TTCATTATCA AATGCAGCCG AGAGACTGAA 
AAGAAATACC CTCAGCCTAA AGGACAGAAA AAAAAGAAGA TAGTGAAATA TGGAATGGGT 
GGTTTGATCA TAGTCTTCCT TATCTGTATA ATCTGGTTTC CCCTTCTCTT CATGTCGTTG 
GTGCGCTCAG TGGTGGGAGT CGTCAACCAC CCTATCGATG TCACGGTTAC TTTTAAACTC 
GGGGGTTATG AGCCTTTATT CACGATGAGT GCACAGCAAC AGTCCATCCA GCCATTCACC 
CCACAGCAAT ATGAAGCGCT TACCTATGAG TTTGAGAGAC AGCCGACTGC CATGCAGTTC 
ATTACTTTGT ATAGTTATGA GGACATAGTG ACTGCGCGAA TTGAGGGAAG TTCAGGATCT 
GTATGGGGAA TCAGCCCTCC AAGCCGTGAG CAGATGAGAA AGGAGCTGCA AAATGGATCC 
TCTGACATTA CACTACGCTT CACCTGGGAT TTTCAGAGGG ATCTGGCAAA AGGTGGCACT 
GTGGAGCACA CATCTGAGAA ACACACTAAG GACTTGGAGC CTGGGAGCCA GGAGAGGATG 
GAGCTGGCAG ATCTTCTTCA AGGAGTACGA GACACCCCTG TCTCTATCCA GCACTTGTTT 
CCAAAGTACA TCCGCGCTCC TAATGGCCCA GAAGCAAATC CAGTGAAACA GCTCCAGCCA 
GATGAAGAAG AAAGTTACCT GGATGTGGAG GTGCAGCTAA ATCGGATGCG AGTTGCAGAG 
GGAAATAGCA CCAGCAGTTT TGTGGAGTGG TGGGTTATTA CTCTAAAAGA CTGCGAGGGA 
GAAGACTGCA ACATACTACC TATGGTGATT TTTAACGATA AAGTTAGCCC TCCTAGCTTA 
GGATTCCTGG CTGGATATGG GATAATGGGT TTGTATGTAT CTATTGTCCT GGTCATTGGG 
AAGTTTGTGC GCGGCTTCTT CAGTGAAATC TCTCACTCCA TCATGTTTGA GGAGCTGCCT 
TGTGTGGATC GGATTCTCAA GCTGTGCCAG GATATCTATG TGGTGCGGGA GCGTGGGGAA 
TTGGAACTGG AAGAGGAACT CTATGCAAAA CTCATCTTCC TGTATCGCTC ACCAGAAACC 
ATGATCAAGT GGACTGTAGA TAAGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817736.2
CDS	820..8169
Translation	MELQVLCGVLFWLLLPGSLLAACLFRYNSLSLVYLFYLLLLPWFAGPNPCSMRGHTGRLMKALLATSILFLCAHLIFQICLYTIPELDDLLGHNCSSWDVLARHVGINRLDLSDIPNMVRQVSPDFGMFVVSALCLGLCNRLVTKKRRQRSSGYFEQEEERTELNLKYESRECDTEFEVGGDLHSVPRDRLLGSWLRKAAYRLLETVEKSLAVLLLALAGITDPSAFSAPYYVVFIGLCTWWACHFPISQLGFNTICVIVGFFTGGHMICLYIYQTPFIQQMFHPEQLWARLFGLHNIISHSNITDCTHDSLNQNLDWPVYVHPGILLLLYYSLLSILKLKSSDKKRISGCDLRKSQQEDMELTDIEPVSKEQYSAGEETKPMLQRMNTEEEAKPQDCTVHVIRRNSNQLPEELETKEPSQFHLMIHVILSQSYICALIAMMVWSITYHSWLTFVLLLWACLIWMVRNRRQFAMLCSPFILMYGVTLCSLQYVWSMDLREELPTQLGFMKLSQLGLVRAPYPCLNMGALLLYTLTFWLLLHQFVTEKFLKRKKFMSPLLEVTVADAGKEKNLLKRFGEQVKSFYAKYWIYVCGGMFIVVSFAGRLVVYKIVYMLLFLLCLVLFQVYYSLWRKLLTFFWWLVVAYTMLVLITIYTFQFEEFPEYWKNLTNLTDEQLGDIGLERFSVSELFTSILIPGFFLLACILQLHYFHRPFMHMTNLAHASCQGSFHSHESEQPKEEEEKAGACSDAAADEEEDEEESAHGADLSKWDLVTQRLSVLSFKFSELLHRLQVFLWRLLELHVLKLVALYSVWVALQEVSVMNFLFVVLWAFAMPFSRFQHMASCLCTIWTSIIIVCKMLYQLTVVNPLEYSSNCTQPPLNNTSLLEDEILNSTLYREPIDPANWFGIRKGYPNLGYIQNHLQILLLLVFEAIVYRHQEFYRKQYQLEKPHIQAVFPDVYREHLDQGLFSCIKYFINYSFYKFGLEICFLLTVNVIGQRMNFMVIFHGCWLIVILTRRQRVNIAKLWPKYCVFLIFFLLWQYMICLGIPPVLCIDYPWRWDTFVPVNSALIKWLYLPDFYTTPKSVNLISDFVLLIGVAQQWKVFINEKKEEWYTMAGENVEEYEAPKDRPNPVPNFINCQSYLDMVKVAVFRYLFWFVLTVVFITGATRISVFGLGYLMSCFYLLLSGTTLLQKPMKAKLVLWDCLILYNITVILSKNMLSLLSCVFVQQMQTNFCWVIQLFSLVCTVKGYYNPQDMDKDRDCSLPVEEAGIIWDSICFLFLLLQRRVFLSCYFRHVVLDLRASSLQTSRGFELFMASIRKNVFFHQESERRSLNQLRKQMERIRAKQERYHEGRRQTDSSQVKEVLHSGDYFLFESDSEEDEESLLEQDQRPPKQSAFQLAYQAWVTSAKSALKERKSRQKERSGKVEMVTVENNEAGGGTESHTQEPREKGEDKDLNEERGGSNVVQRILDVLKFLWVLLLAMVDGLINWLGTLTKQHVDMSTVLWVERYIITQKLSKGEDINRRDLEKVYETGQDVREPRISSPPTDMSTSIASSGCEEEELICSTAECTENITQLSTGYNTRSNSLDSIRGFPEIKPSHRSRTASELLLSRPLDFPELSESQHFFQSQSRILRLLLALYNFVAAHSELLCYFIIILNNMVSASLISLFLPILIFLWGMLSIHKPSKRFWMTAIIFTEVMVVIKYLFQFGFFPWNNYTILVRNEGKPFFPPRVLGLEKSDSYIKYDLIQLLMLFFHRSLLMSYGLWDRKDLDLEERTPGKDKGSKTEIQASQDLEPAGGSSSSLRQRKRKESQGSVQERDSLKEKSLDVQKKKKGRVALRVKIMGMKMQQFFACIVRNIYQPVKAFFHDILHTKNRAATDVYAFMFLADVVDFVIIVFGFWAFGKHSAAADIASSLSDDQVPEAFLVMVLIQFSTMVIDRALYLRKTVLGKLIFQVILVIGIHVWMFFILPAVTERMFNQNTVAQLWYFVKCIYFALSAYQIRCGYPTRILGNFLTKKYNHLNLFLFQGFRLVPFLVELRAVMDWVWTDTTLSLSNWMCVEDIYANIFIIKCSRETEKKYPQPKGQKKKKIVKYGMGGLIIVFLICIIWFPLLFMSLVRSVVGVVNHPIDVTVTFKLGGYEPLFTMSAQQQSIQPFTPQQYEALTYEFERQPTAMQFITLYSYEDIVTARIEGSSGSVWGISPPSREQMRKELQNGSSDITLRFTWDFQRDLAKGGTVEHTSEKHTKDLEPGSQERMELADLLQGVRDTPVSIQHLFPKYIRAPNGPEANPVKQLQPDEEESYLDVEVQLNRMRVAEGNSTSSFVEWWVITLKDCEGEDCNILPMVIFNDKVSPPSLGFLAGYGIMGLYVSIVLVIGKFVRGFFSEISHSIMFEELPCVDRILKLCQDIYVVRERGELELEEELYAKLIFLYRSPETMIKWTVDKD

Target ORF information:

RefSeq Version	XM_012962282.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis piezo-type mechanosensitive ion channel component 1 (piezo1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012962282.3

ORF Insert Sequence:

ATGGAACTGC AAGTCCTCTG TGGGGTCCTG TTTTGGCTCC TTCTGCCCGG CTCCCTCTTG 
GCAGCCTGCT TATTCCGTTA CAACTCCTTG TCACTGGTGT ACCTGTTTTA TCTTCTGCTT 
CTGCCATGGT TTGCTGGACC AAATCCCTGC AGCATGAGAG GTCACACAGG ACGTCTGATG 
AAAGCGTTAC TTGCTACCAG CATCCTCTTC CTCTGTGCTC ATCTCATCTT CCAGATCTGC 
CTGTACACAA TACCTGAACT GGATGACCTG CTAGGGCACA ACTGCAGCAG CTGGGATGTG 
TTGGCTCGTC ACGTAGGTAT AAACAGGCTG GATCTTTCTG ATATCCCCAA CATGGTGCGA 
CAAGTGTCCC CAGATTTTGG CATGTTTGTC GTGTCAGCAT TGTGCCTTGG CTTGTGTAAT 
CGCCTAGTGA CCAAGAAGAG ACGACAGAGG TCTTCAGGCT ATTTTGAGCA GGAAGAAGAG 
AGGACTGAAC TTAACCTAAA ATATGAAAGT AGAGAGTGTG ATACAGAGTT TGAGGTGGGG 
GGAGACTTAC ATTCTGTTCC ACGAGACCGC CTTCTTGGGT CCTGGCTTAG AAAGGCAGCA 
TACAGGCTTC TGGAAACAGT GGAGAAATCA CTTGCTGTCT TACTCTTGGC TCTTGCAGGT 
ATCACTGATC CCTCTGCTTT CTCAGCCCCA TATTATGTGG TTTTCATTGG TCTCTGCACA 
TGGTGGGCCT GCCATTTTCC CATTAGCCAA CTAGGCTTTA ATACTATCTG TGTCATTGTT 
GGGTTCTTCA CTGGAGGTCA CATGATTTGC CTTTATATTT ACCAGACACC TTTTATCCAA 
CAAATGTTTC ATCCTGAACA GTTATGGGCA AGGTTGTTTG GTCTGCATAA CATCATCAGT 
CACTCCAACA TTACAGACTG TACCCATGAC TCTCTGAATC AAAACCTTGA CTGGCCTGTG 
TATGTTCATC CCGGAATCTT ATTACTGCTG TACTACTCCC TATTATCAAT ACTGAAACTG 
AAGAGCTCTG ACAAGAAAAG GATTTCAGGG TGTGACCTTC GGAAATCTCA GCAGGAGGAT 
ATGGAGTTGA CTGATATTGA GCCAGTTTCA AAGGAGCAAT ATAGTGCGGG AGAAGAGACA 
AAACCAATGC TTCAGAGGAT GAACACTGAA GAAGAGGCTA AACCCCAAGA CTGCACTGTT 
CATGTGATTA GAAGAAATTC TAATCAACTG CCAGAAGAAC TTGAAACCAA GGAGCCATCA 
CAATTTCACC TGATGATTCA TGTAATACTG AGCCAAAGCT ATATCTGTGC GCTTATTGCA 
ATGATGGTGT GGAGCATTAC ATATCACAGC TGGCTGACTT TTGTGCTGCT GCTCTGGGCA 
TGTTTAATCT GGATGGTGAG GAACAGGCGC CAGTTTGCAA TGCTGTGCTC TCCCTTTATT 
TTGATGTATG GAGTGACTCT CTGCAGCCTG CAGTATGTCT GGAGTATGGA CTTGAGAGAA 
GAGCTTCCGA CACAACTTGG TTTTATGAAG CTGTCACAGC TGGGACTGGT TCGCGCACCA 
TACCCCTGCC TTAACATGGG AGCACTGCTA CTTTACACGC TGACATTTTG GCTTCTGCTG 
CACCAATTTG TAACAGAAAA ATTTCTAAAA CGTAAGAAGT TTATGTCCCC ACTCCTGGAG 
GTCACAGTGG CAGATGCTGG CAAAGAAAAG AACCTGCTAA AACGTTTTGG GGAGCAAGTA 
AAAAGCTTCT ATGCAAAATA CTGGATCTAT GTGTGTGGAG GCATGTTTAT TGTGGTCAGC 
TTTGCTGGTA GGCTGGTAGT TTACAAAATA GTCTACATGC TTCTGTTCCT ACTATGCTTG 
GTGTTATTTC AGGTGTATTA CTCTTTGTGG AGGAAGCTGC TGACTTTTTT CTGGTGGTTA 
GTTGTAGCAT ACACAATGTT GGTTCTTATC ACCATCTACA CCTTTCAATT TGAAGAGTTT 
CCTGAGTACT GGAAAAACTT GACCAACCTT ACAGATGAGC AGTTGGGTGA TATTGGGCTT 
GAGAGGTTTA GTGTGTCGGA ACTTTTTACC AGCATCCTTA TCCCAGGCTT CTTCCTCCTT 
GCCTGCATCC TCCAGTTACA CTACTTTCAT CGCCCTTTTA TGCACATGAC CAACTTGGCA 
CATGCCTCAT GCCAAGGAAG TTTCCATAGC CACGAGAGTG AGCAGCCAAA GGAAGAAGAA 
GAGAAAGCTG GTGCATGCAG TGATGCTGCT GCTGATGAGG AGGAGGATGA GGAAGAATCT 
GCACATGGAG CTGATCTAAG TAAATGGGAC CTAGTTACAC AGCGCCTTTC TGTCCTGTCC 
TTTAAGTTCT CTGAGCTGCT GCACCGATTA CAGGTTTTCC TTTGGAGGCT TTTGGAGCTC 
CATGTGTTGA AACTGGTGGC TTTGTATAGC GTGTGGGTTG CCCTGCAGGA GGTATCTGTG 
ATGAATTTCC TTTTTGTGGT CCTATGGGCT TTTGCTATGC CTTTCTCCCG ATTCCAGCAC 
ATGGCTTCCT GTCTTTGCAC CATCTGGACC TCTATAATTA TTGTTTGCAA AATGCTTTAC 
CAGCTTACTG TTGTCAACCC TCTGGAGTAC TCTAGCAACT GTACTCAGCC TCCTCTAAAT 
AACACCAGTC TCCTAGAAGA CGAGATTTTG AACTCCACCC TTTATAGAGA ACCTATAGAT 
CCAGCAAACT GGTTTGGTAT CAGAAAAGGT TATCCAAACC TAGGTTACAT ACAGAATCAC 
CTGCAAATAC TCCTACTTTT GGTATTTGAG GCAATTGTTT ATCGGCATCA GGAGTTTTAC 
CGTAAACAAT ACCAGCTTGA GAAGCCACAC ATTCAGGCTG TATTTCCAGA TGTCTACAGA 
GAGCACTTGG ACCAAGGCTT GTTCAGCTGT ATCAAATATT TTATCAACTA TTCCTTTTAT 
AAGTTTGGTC TTGAGATCTG CTTCCTTCTT ACTGTGAATG TGATTGGTCA GAGGATGAAC 
TTTATGGTAA TCTTTCATGG CTGCTGGCTG ATAGTGATTC TTACTCGACG ACAGCGAGTC 
AACATTGCAA AATTATGGCC TAAATACTGT GTGTTCCTAA TCTTTTTCCT GTTGTGGCAG 
TACATGATAT GTCTGGGCAT TCCTCCGGTG CTTTGTATAG ATTATCCCTG GAGGTGGGAC 
ACATTTGTTC CTGTCAACTC TGCACTGATA AAGTGGTTAT ATCTTCCAGA TTTCTATACT 
ACACCTAAAT CTGTCAACCT TATCAGTGAT TTTGTTTTGC TGATTGGTGT GGCCCAGCAG 
TGGAAGGTGT TTATTAATGA GAAGAAGGAA GAATGGTACA CTATGGCAGG GGAAAATGTG 
GAAGAGTATG AAGCTCCAAA AGACAGGCCC AACCCTGTAC CTAACTTCAT CAATTGTCAG 
TCTTATCTGG ATATGGTGAA AGTAGCAGTG TTTCGGTACC TATTCTGGTT TGTCTTGACT 
GTGGTCTTTA TAACAGGGGC CACTCGCATC AGTGTTTTTG GCCTAGGATA TTTGATGTCC 
TGTTTCTACC TTCTTCTGTC TGGCACAACC CTTCTGCAAA AGCCAATGAA AGCCAAGCTG 
GTGCTTTGGG ACTGTTTGAT CCTCTACAAC ATCACTGTTA TACTGTCCAA GAACATGCTG 
TCGCTCCTGT CTTGTGTATT TGTTCAACAA ATGCAAACCA ACTTTTGCTG GGTGATCCAG 
CTCTTCAGCC TAGTTTGCAC AGTTAAGGGT TACTACAATC CCCAAGATAT GGATAAAGAC 
CGGGATTGCT CTCTGCCAGT GGAAGAGGCC GGAATCATTT GGGATAGCAT CTGCTTCTTA 
TTTCTCCTTC TCCAACGAAG AGTTTTTTTG AGTTGCTACT TTCGGCATGT TGTTCTTGAC 
CTTCGTGCCT CCTCCCTTCA AACATCAAGA GGTTTTGAGC TCTTCATGGC CAGCATCAGA 
AAGAATGTGT TTTTCCACCA AGAATCTGAG AGACGATCTC TTAACCAGCT CAGGAAGCAA 
ATGGAGAGAA TCCGAGCAAA GCAAGAACGC TATCATGAAG GCAGAAGACA AACTGATAGC 
AGTCAAGTAA AGGAAGTGCT GCATTCAGGA GATTATTTTC TGTTCGAATC AGACAGTGAA 
GAGGATGAAG AGTCTTTATT GGAGCAGGAT CAGAGGCCAC CCAAGCAGAG TGCTTTTCAG 
CTTGCATACC AGGCATGGGT TACAAGTGCC AAATCTGCGC TAAAGGAAAG AAAGAGTCGG 
CAGAAAGAGA GATCTGGAAA GGTAGAAATG GTCACGGTAG AGAATAATGA AGCAGGAGGG 
GGGACAGAGT CTCATACCCA GGAACCAAGG GAGAAAGGAG AGGATAAAGA TCTGAATGAG 
GAGAGAGGAG GAAGCAATGT GGTACAGCGT ATCCTTGACG TGTTGAAGTT TCTCTGGGTT 
CTTTTGCTTG CCATGGTGGA TGGACTGATC AATTGGCTAG GAACCCTTAC AAAGCAGCAT 
GTTGATATGT CCACAGTACT GTGGGTAGAG AGATATATCA TAACCCAAAA ACTGTCTAAG 
GGAGAAGATA TCAACCGCAG GGATTTGGAA AAGGTATATG AGACAGGGCA GGATGTTAGG 
GAGCCTAGGA TTTCATCTCC TCCAACTGAT ATGTCAACTA GCATTGCTTC AAGTGGCTGT 
GAGGAAGAGG AGCTTATCTG CAGCACAGCA GAGTGTACGG AAAATATAAC ACAACTCAGC 
ACCGGGTACA ACACACGAAG TAACAGCTTG GACTCTATTA GGGGGTTTCC GGAAATCAAG 
CCAAGTCATC GTTCTCGTAC TGCTAGTGAA TTGCTGCTTT CCAGGCCACT TGATTTCCCA 
GAGCTCTCCG AGTCTCAGCA CTTCTTTCAG TCTCAGAGTC GTATTCTGAG GCTCCTGTTG 
GCCCTCTATA ACTTTGTGGC TGCTCACTCT GAGCTGCTTT GTTACTTCAT CATCATTCTC 
AACAACATGG TGAGCGCTTC CCTGATATCA CTGTTCCTTC CAATTCTCAT CTTCCTGTGG 
GGTATGCTGT CCATCCACAA ACCCAGTAAG CGCTTCTGGA TGACTGCTAT CATTTTTACT 
GAGGTTATGG TAGTAATAAA ATACCTCTTC CAGTTTGGTT TCTTCCCATG GAATAATTAT 
ACCATTCTGG TACGCAACGA AGGGAAACCA TTTTTTCCTC CACGTGTCCT GGGACTTGAG 
AAGAGTGACT CGTACATCAA ATATGATCTG ATCCAGCTCT TGATGCTCTT TTTCCATCGC 
TCACTGCTCA TGAGTTATGG TCTTTGGGAT CGTAAAGACT TAGACCTTGA AGAGAGAACA 
CCCGGGAAGG ATAAAGGGAG CAAAACAGAG ATTCAGGCAT CCCAGGATCT GGAGCCAGCA 
GGAGGCAGTA GCTCTTCCCT GCGTCAGCGC AAAAGAAAAG AATCCCAGGG CAGTGTGCAA 
GAAAGAGATT CCTTAAAAGA GAAATCACTA GATGTGCAGA AGAAGAAAAA AGGAAGAGTG 
GCTCTTAGGG TGAAGATCAT GGGAATGAAA ATGCAACAGT TCTTTGCCTG CATAGTGCGG 
AATATATATC AGCCAGTGAA AGCTTTTTTC CATGATATTC TACATACCAA GAACCGTGCT 
GCAACAGATG TTTATGCCTT CATGTTCCTC GCAGATGTGG TTGACTTTGT GATCATCGTA 
TTTGGGTTTT GGGCTTTTGG AAAACACTCT GCTGCTGCCG ACATAGCATC ATCTCTCTCT 
GATGACCAAG TACCAGAAGC TTTCCTAGTC ATGGTCCTCA TACAGTTTTC CACCATGGTG 
ATAGATCGTG CTCTATACCT GCGGAAGACT GTGCTTGGAA AGCTCATCTT TCAAGTTATT 
CTGGTGATTG GGATACATGT CTGGATGTTC TTTATCCTAC CAGCAGTCAC AGAAAGGATG 
TTCAATCAGA ACACTGTGGC CCAGCTCTGG TATTTTGTTA AGTGCATTTA CTTTGCCCTG 
TCTGCCTACC AGATCCGCTG TGGGTATCCT ACCCGCATCC TGGGCAACTT CCTTACCAAG 
AAATACAACC ACCTCAACCT ATTCCTATTT CAGGGGTTTC GCTTGGTTCC GTTTCTAGTA 
GAGCTCAGGG CTGTAATGGA CTGGGTTTGG ACTGATACCA CCTTGTCCTT GTCCAACTGG 
ATGTGCGTGG AAGACATTTA TGCTAATATC TTCATTATCA AATGCAGCCG AGAGACTGAA 
AAGAAATACC CTCAGCCTAA AGGACAGAAA AAAAAGAAGA TAGTGAAATA TGGAATGGGT 
GGTTTGATCA TAGTCTTCCT TATCTGTATA ATCTGGTTTC CCCTTCTCTT CATGTCGTTG 
GTGCGCTCAG TGGTGGGAGT CGTCAACCAC CCTATCGATG TCACGGTTAC TTTTAAACTC 
GGGGGTTATG AGCCTTTATT CACGATGAGT GCACAGCAAC AGTCCATCCA GCCATTCACC 
CCACAGCAAT ATGAAGCGCT TACCTATGAG TTTGAGAGAC AGCCGACTGC CATGCAGTTC 
ATTACTTTGT ATAGTTATGA GGACATAGTG ACTGCGCGAA TTGAGGGAAG TTCAGGATCT 
GTATGGGGAA TCAGCCCTCC AAGCCGTGAG CAGATGAGAA AGGAGCTGCA AAATGGATCC 
TCTGACATTA CACTACGCTT CACCTGGGAT TTTCAGAGGG ATCTGGCAAA AGGTGGCACT 
GTGGAGCACA CATCTGAGAA ACACACTAAG GACTTGGAGC CTGGGAGCCA GGAGAGGATG 
GAGCTGGCAG ATCTTCTTCA AGGAGTACGA GACACCCCTG TCTCTATCCA GCACTTGTTT 
CCAAAGTACA TCCGCGCTCC TAATGGCCCA GAAGCAAATC CAGTGAAACA GCTCCAGCCA 
GATGAAGAAG AAAGTTACCT GGATGTGGAG GTGCAGCTAA ATCGGATGCG AGTTGCAGAG 
GGAAATAGCA CCAGCAGTTT TGTGGAGTGG TGGGTTATTA CTCTAAAAGA CTGCGAGGGA 
GAAGACTGCA ACATACTACC TATGGTGATT TTTAACGATA AAGTTAGCCC TCCTAGCTTA 
GGATTCCTGG CTGGATATGG GATAATGGGT TTGTATGTAT CTATTGTCCT GGTCATTGGG 
AAGTTTGTGC GCGGCTTCTT CAGTGAAATC TCTCACTCCA TCATGTTTGA GGAGCTGCCT 
TGTGTGGATC GGATTCTCAA GCTGTGCCAG GATATCTATG TGGTGCGGGA GCGTGGGGAA 
TTGGAACTGG AAGAGGAACT CTATGCAAAA CTCATCTTCC TGTATCGCTC ACCAGAAACC 
ATGATCAAGT GGACTGTAGA TAAGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

piezo1 ( XM_002933675.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002933721.2 Xenopus tropicalis piezo-type mechanosensitive ion channel component 1 (piezo1), transcript variant X1, mRNA.

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CloneID	OXa08835
Clone ID Related Accession (Same CDS sequence)	XM_002933675.4
Accession Version	XM_002933675.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7425bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	piezo-type mechanosensitive ion channel component 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002933675.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACTGC AAGTCCTCTG TGGGGTCCTG TTTTGGCTCC TTCTGCCCGG CTCCCTCTTG 
GCAGCCTGCT TATTCCGTTA CAACTCCTTG TCACTGGTGT ACCTGTTTTA TCTTCTGCTT 
CTGCCATGGT TTGCTGGACC AAATCCCTGC AGCATGAGAG GTCACACAGG ACGTCTGATG 
AAAGCGTTAC TTGCTACCAG CATCCTCTTC CTCTGTGCTC ATCTCATTTT CCAGATCTGC 
CTGTACACAA TACCTGAACT GGATGACCTG CTAGGGCACA ACTGCAGCAG CTGGGATGTG 
TTGGCTCGTC ACGTAGGTAT AAACAGGCTG GATCTTTCTG ATATCCCCAA CATGGTGCGA 
CAAGTGTCCC CAGATTTTGG CATGTTTGTC GTGTCAGCAT TGTGCCTTGG CTTGTGTAAT 
CGCCTAGTGA CCAAGAAGAG ACGACAGAGG TCTTCAGGCT ATTTTGAGCA GGAAGAAGAG 
AGGACTGAAC TTAACCTAAA ATATGAAAGT AGAGAGTGTG ATACAGAGTT TGAGGTGGGG 
GGAGACTTAC ATTCTGTTCC ACGAGACCGC CTTCTTGGGT CCTGGCTTAG AAAGGCAGCA 
TACAGGCTTC TGGAAACAGT GGAGAAATCA CTTGCTGTCT TACTCTTGGC TCTTGCAGGT 
ATCACTGATC CCTCTGCTTT CTCAGCCCCA TATTATGTGG TTTTCATTGG TCTCTGCACA 
TGGTGGGCCT GCCATTTTCC CATTAGCCAA CTAGGCTTTA ATACTATCTG TGTCATTGTT 
GGGTTCTTCA CTGGAGGTCA CATGATTTGC CTTTATATTT ACCAGACACC TTTTATCCAA 
CAAATGTTTC ATCCTGAACA GTTATGGGCA AGGTTGTTTG GTCTGCATAA CATCATCAGT 
CACTCCAACA TTACAGACTG TACCCATGAC TCTCTGAATC AAAACCTTGA CTGGCCTGTG 
TATGTTCATC CCGGAATCTT ATTACTGCTG TACTACTCCC TATTATCAAT ACTGAAACTG 
AAGAGCTCTG ACAAGAAAAG GATTTCAGGG TGTGACCTTC GGAAATCTCA GCAGGAGGAT 
ATGGAGTTGA CCGATATTGA GCCAGTTTCA AAGGAGCAAT ATAGTGCGGG AGAAGAGACA 
AAACCAATGC TTCAGAGGAT GAACACTGAA GAAGAGGCTA AACCCCAAGA CTGCACTGTT 
CATGTGATTA GAAGAAATTC TAATCAACTG CCAGAAGAAC TTGAAACCAA GGAGCCATCA 
CAATTTCACC TGATGATTCA TGTAATACTG AGCCAAAGCT ATATCTGTGC GCTTATTGCA 
ATGATGGTGT GGAGCATTAC ATATCACAGC TGGCTGACTT TTGTGCTGCT GCTCTGGGCA 
TGTTTAATCT GGATGGTGAG GAACAGGCGC CAGTTTGCAA TGCTGTGCTC TCCCTTTATT 
TTGATGTATG GAGTGACTCT CTGCAGCCTG CAGTATGTCT GGAGTATGGA CTTGAGAGAA 
GAGCTTCCGA CACAACTTGG TTTTATGAAG CTGTCACAGC TGGGACTGGT TCGCGCACCA 
TACCCCTGCC TTAACATGGG AGCACTGCTA CTTTACACGC TGACATTTTG GCTTCTGCTG 
CACCAATTTG TAACAGAAAA ATTTCTAAAA CGTAAGAAGT TTATGTCCCC ACTCCTGGAG 
GTCACAGTGG CAGATGCTGG CAAAGAAAAG AACCTGCTAA AACGTTTTGG GGAGCAAGTA 
AAAAGCTTCT ATGCAAAATA CTGGATCTAT GTGTGTGGAG GCATGTTTAT TGTGGTCAGC 
TTTGCTGGTA GGCTGGTAGT TTACAAAATA GTCTACATGC TTCTGTTCCT ACTATGCTTG 
GTGTTATTTC AGGTGTATTA CTCTTTGTGG AGGAAGCTGC TGACTTTTTT CTGGTGGTTA 
GTTGTAGCAT ACACAATGTT GGTTCTTATC ACCATCTACA CCTTTCAATT TGAAGAGTTT 
CCTGAGTACT GGAAAAACTT GACCAACCTT ACAGATGAGC AGTTGGGTGA TATTGGGCTT 
GAGAGGTTTA GTGTGTCGGA ACTTTTTACC AGCATCCTTA TCCCAGGCTT CTTCCTCCTT 
GCCTGCATCC TCCAGTTACA CTACTTTCAT CGCCCTTTTA TGCACATGAC CAACTTGGCA 
CATGCCTCAT CCCAAGGAAG TTTCCATAGC CACGAGAGTG AGCAGCCAAA GGAAGAAGAA 
GAGAAAGCTG GTGCATGCAG TGATGCTGCT GCTGATGAGG AGGAGGATGA GGAAGAATCT 
GCACATGGAG CTGATCTAAG TAAATGGGAC CTAGTTACAC AGCGCCTTTC TGTCCTGTCC 
TTTAAGTTCT CTGAGCTGCT GCACCGATTA CAGGTTTTCC TTTGGAGGCT TTTGGAGCTC 
CATGTGTTGA AACTGGTGGC TTTGTATAGC GTGTGGGTTG CCCTGCAGGA GGTATCTGTG 
ATGAATTTCC TTTTTGTGGT CCTATGGGCT TTTGCTATGC CTTTCTCCCG ATTCCAGCAC 
ATGGCTTCCT GTCTTTGCAC CATCTGGACC TCTATAATTA TTGTTTGCAA AATGCTTTAC 
CAGCTTACTG TTGTCAACCC TCTGGAGTAC TCTAGCAACT GTACTCAGCC TCTTCTAAAT 
AACACCAGTC TCCTAGAAGA CGAGATTTTG AACTCCACCC TTTATAGAGA ACCTATAGAT 
CCAGCAAACT GGTTTGGTAT CAGAAAAGGT TATCCAAACC TAGGTTACAT ACAGAATCAC 
CTGCAAATAC TCCTACTTTT GGTATTTGAG GCAATTGTTT ATCGGCATCA GGAGTTTTAC 
CGTAAACAAT ACCAGCTTGA GAAGCCACAC ATTCAGGCTG TATTTCCAGA TGTCTACAGA 
GAGCACTTGG ACCAAGGCTT GTTCAGCTGT ATCAAATATT TTATCAACTA TTCCTTTTAT 
AAGTTTGGTC TTGAGATCTG CTTCCTTCTT ACTGTGAATG TGATTGGTCA GAGGATGAAC 
TTTATGGTAA TCTTTCATGG CTGCTGGCTG ATAGTGATTC TTACTCGACG ACAGCGAGTC 
AACATTGCAA AATTATGGCC TAAATACTGT GTGTTCCTAA TCTTTTTCCT GTTGTGGCAG 
TACATGATAT GTCTGGGCAT TCCTCCGGTG CTTTGTATAG ATTATCCCTG GAGGTGGGAC 
ACATTTGTTC CTGTCAACTC TGCACTGATA AAGTGGTTAT ATCTTCCAGA TTTCTATACT 
ACACCTAAAT CTGTCAACCT TATCAGTGAT TTTGTTTTGC TGATTGGTGT GGCCCAGCAG 
TGGAAGGTGT TTATTAATGA GAAGAAGGAA GAATGGTACA CTATGGCAGG GGAAAATGTG 
GAAGAGTATG AAGCTCCAAA AGACAGGCCC AACCCTGTAC CTAACTTCAT CAATTGTCAG 
TCTTATCTGG ATATGGTGAA AGTAGCAGTG TTTCGGTACC TATTCTGGTT TGTCTTGACT 
GTGGTCTTTA TAACAGGGGC CACTCGCATC AGTGTTTTTG GCCTAGGATA TTTGATGTCC 
TGTTTCTACC TTCTTCTGTC TGGCACAACC CTTCTGCAAA AGCCAATGAA AGCCAAGCTG 
GTGCTTTGGG ACTGTTTGAT CCTCTACAAC ATCACTGTTA TACTGTCCAA GAACATGCTG 
TCGCTCCTGT CTTGTGTATT TGTTCAACAA ATGCAAACCA ACTTTTGCTG GGTGATCCAG 
CTCTTCAGCC TAGTTTGCAC AGTTAAGGGT TACTACAATC CCCAAGATAT GGATAAAGAC 
CGGGATTGCT CTCTGCCAGT GGAAGAGGCC GGAATCATTT GGGATAGCAT CTGCTTCTTA 
TTTCTCCTTC TCCAACGAAG AGTTTTTTTG AGTTGCTACT TTCGGCATGT TGTTCTTGAC 
CTTCGTGCCT CCTCCCTTCA AACATCAAGA GGTTTTGAGC TCTTCATGGC CAGCATCAGA 
AAGAATGTGT TTTTCCACCA AGAATCTGAG AGACGATCTC TTAACCAGCT CAGGAAGCAA 
ATGGAGAGAA TCCGAGCAAA GCAAGAACGC TATCATGAAG GCAGAAGACA AACTGATAGC 
AGTCAAGTAA AGGAAGGTGA GCCTGGCAGC AAGAAAGGGT CCAGTAGGCA AAAGATTCAT 
TGGAGCCGCC CATGGTTAGA CCACGCCTCA GTGCTGCATT CAGGAGATTA TTTTCTGTTC 
GAATCAGACA GTGAAGAGGA TGAAGAGTCT TTATTGGAGC AGGATCAGAG GCCACCCAAG 
CAGAGTGCTT TTCAGCTTGC ATACCAGGCA TGGGTTACAA GTGCCAAATC TGCGCTAAAG 
GAAAGAAAGA GTCGGCAGAA AGAGAGATCT GGAAAGGTAG AAATGGTCAC GGTAGAGAAT 
AATGAAGCAG GAGGGGGGAC AGAGTCTCAT ACCCAGGAAC CAAGGGAGAA AGGAGAGGAT 
AAAGATCTGA ATGAGGAGAG AGGAGGAAGC AATGTGGTAC AGCGTATCCT TGACGTGTTG 
AAGTTTCTCT GGGTTCTTTT GCTTGCCATG GTGGATGGAC TGATCAATTG GCTAGGAACC 
CTTACAAAGC AGCATGTTGA TATGTCCACA GTACTGTGGG TAGAGAGATA TATCATAACC 
CAAAAACTGT CTAAGGGAGA AGATATCAAC CGCAGGGATT TGGAAAAGGT ATATGAGACA 
GGGCAGGATG TTAGGGAGCC TAGGATTTCA TCTCCTCCAA CTGATATGTC AACTAGCATT 
GCTTCAAGTG GCTGTGAGGA AGAGGAGCTT ATCTGCAGCA CAGCAGAGTG TACGGAAAAT 
ATAACACAAC TCAGCACCGG GTACAACACA CGAAGTAACA GCTTGGACTC TATTAGGGGG 
TTTCCGGAAA TCAAGCCAAG TCATCGTTCT CGTACTGCTA GTGAATTGCT GCTTTCCAGG 
CCACTTGATT TCCCAGAGCT CTCCGAGTCT CAGCACTTCT TTCAGTCTCA GAGTCGTATT 
CTGAGGCTCC TGTTGGCCCT CTATAACTTT GTGGCTGCTC ACTCTGAGCT GCTTTGTTAC 
TTCATCATCA TTCTCAACAA CATGGTGAGC GCTTCCCTGA TATCACTGTT CCTTCCAATT 
CTCATCTTCC TGTGGGGTAT GCTGTCCATC CACAAACCCA GTAAGCGCTT CTGGATGACT 
GCTATCATTT TTACTGAGGT TATGGTAGTA ATAAAATACC TCTTCCAGTT TGGTTTCTTC 
CCATGGAATA ATTATACCAT TCTGGTACGC AACGAAGGGA AACCATTTTT TCCTCCACGT 
GTCCTGGGAC TTGAGAAGAG TGACTCGTAC ATCAAATATG ATCTGATCCA GCTCTTGATG 
CTCTTTTTCC ATCGCTCACT GCTCATGAGT TATGGTCTTT GGGATCGTAA AGACTTAGAC 
CTTGAAGAGA GAACACCCGG GAAGGATAAA GGGAGCAAAA CAGAGATTCA GGCATCCCAG 
GATCTGGAGC CAGCAGGAGG CAGTAGCTCT TCCCTGCGTC AGCGCAAAAG AAAAGAATCC 
CAGGGCAGTG TGCAAGAAAG AGATTCTTTA AAAGAGAAAT CACTAGATGT GCAGAAGAAG 
AAAAAAGGAA GAGTGGCTCT TAGGGTGAAG ATCATGGGAA TGAAAATGCA ACAGTTCTTT 
GCCTGCATAG TGCGGAATAT ATATCAGCCA GTGAAAGCTT TTTTCCATGA TATTCTACAT 
ACCAAGAACC GTGCTGCAAC AGATGTTTAT GCCTTCATGT TCCTCGCAGA TGTGGTTGAC 
TTTGTGATCA TCGTATTTGG GTTTTGGGCT TTTGGAAAAC ACTCTGCTGC TGCCGACATA 
GCATCATCTC TCTCTGATGA CCAAGTACCA GAAGCTTTCC TAGTCATGGT CCTCATACAG 
TTTTCCACCA TGGTGATAGA TCGTGCTCTA TACCTGCGGA AGACTGTGCT TGGAAAGCTC 
ATCTTTCAAG TTATTCTGGT GATTGGGATA CATGTCTGGA TGTTCTTTAT CCTACCAGCA 
GTCACAGAAA GGATGTTCAA TCAGAACACT GTGGCCCAGC TCTGGTATTT TGTTAAGTGC 
ATTTACTTTG CCCTGTCTGC CTACCAGATC CGCTGTGGGT ATCCTACCCG CATCCTGGGC 
AACTTCCTTA CCAAGAAATA CAACCACCTC AACCTATTCC TATTTCAGGG GTTTCGCTTG 
GTTCCGTTTC TAGTAGAGCT CAGGGCTGTA ATGGACTGGG TTTGGACTGA TACCACCTTG 
TCCTTGTCCA ACTGGATGTG CGTGGAAGAC ATTTATGCTA ATATCTTCAT TATCAAATGC 
AGCCGAGAGA CTGAAAAGAA ATACCCTCAG CCTAAAGGAC AGAAAAAAAA GAAGATAGTG 
AAATATGGAA TGGGTGGTTT GATCATAGTC TTCCTTATCT GTATAATCTG GTTTCCCCTT 
CTCTTCATGT CGTTGGTGCG CTCAGTGGTG GGAGTCGTCA ACCACCCTAT CGATGTCACG 
GTTACTTTTA AACTCGGGGG TTATGAGCCT TTATTCACGA TGAGTGCACA GCAACAGTCC 
ATCCAGCCAT TCACCCCACA GCAATATGAA GCGCTTACCT ATGAGTTTGA GAGACAGCCG 
ACTGCCATGC AGTTCATTAC TTTGTATAGT TATGAGGACA TAGTGACTGC GCGAATTGAG 
GGAAGTTCAG GATCTGTATG GGGAATCAGC CCTCCAAGCC GTGAGCAGAT GAGAAAGGAG 
CTGCAAAATG GATCCTCTGA CATTACACTA CGCTTCACCT GGGATTTTCA GAGGGATCTG 
GCAAAAGGTG GCACTGTGGA GCACACATCT GAGAAACACA CTAAGGACTT GGAGCCTGGG 
AGCCAGGAGA GGATGGAGCT GGCAGATCTT CTTCAAGGAG TACGAGACAC CCCTGTCTCT 
ATCCAGCACT TGTTTCCAAA GTACATCCGC GCTCCTAATG GCCCAGAAGC AAATCCAGTG 
AAACAGCTCC AGCCAGATGA AGAAGAAAGT TACCTGGATG TGGAGGTGCA GCTAAATCGG 
ATGCGAGTTG CAGAGGGAAA TAGCACCAGC AGTTTTGTGG AGTGGTGGGT TATTACTCTA 
AAAGACTGCG AGGGAGAAGA CTGCAACATA CTACCTATGG TGATTTTTAA CGATAAAGTC 
AGCCCTCCTA GCTTAGGATT CCTGGCTGGA TATGGGATAA TGGGTTTGTA TGTATCTATT 
GTCCTGGTCA TTGGGAAGTT TGTGCGCGGC TTCTTCAGTG AAATCTCTCA CTCCATCATG 
TTTGAGGAGC TGCCTTGTGT GGATCGGATT CTCAAGCTGT GCCAGGATAT CTATGTGGTG 
CGGGAGCGTG GGGAATTGGA ACTGGAAGAG GAACTCTATG CAAAACTCAT CTTCCTGTAT 
CGCTCACCAG AAACCATGAT CAAGTGGACT GTAGATAAGG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002933721.2
CDS	1200..8624
Translation	MELQVLCGVLFWLLLPGSLLAACLFRYNSLSLVYLFYLLLLPWFAGPNPCSMRGHTGRLMKALLATSILFLCAHLIFQICLYTIPELDDLLGHNCSSWDVLARHVGINRLDLSDIPNMVRQVSPDFGMFVVSALCLGLCNRLVTKKRRQRSSGYFEQEEERTELNLKYESRECDTEFEVGGDLHSVPRDRLLGSWLRKAAYRLLETVEKSLAVLLLALAGITDPSAFSAPYYVVFIGLCTWWACHFPISQLGFNTICVIVGFFTGGHMICLYIYQTPFIQQMFHPEQLWARLFGLHNIISHSNITDCTHDSLNQNLDWPVYVHPGILLLLYYSLLSILKLKSSDKKRISGCDLRKSQQEDMELTDIEPVSKEQYSAGEETKPMLQRMNTEEEAKPQDCTVHVIRRNSNQLPEELETKEPSQFHLMIHVILSQSYICALIAMMVWSITYHSWLTFVLLLWACLIWMVRNRRQFAMLCSPFILMYGVTLCSLQYVWSMDLREELPTQLGFMKLSQLGLVRAPYPCLNMGALLLYTLTFWLLLHQFVTEKFLKRKKFMSPLLEVTVADAGKEKNLLKRFGEQVKSFYAKYWIYVCGGMFIVVSFAGRLVVYKIVYMLLFLLCLVLFQVYYSLWRKLLTFFWWLVVAYTMLVLITIYTFQFEEFPEYWKNLTNLTDEQLGDIGLERFSVSELFTSILIPGFFLLACILQLHYFHRPFMHMTNLAHASSQGSFHSHESEQPKEEEEKAGACSDAAADEEEDEEESAHGADLSKWDLVTQRLSVLSFKFSELLHRLQVFLWRLLELHVLKLVALYSVWVALQEVSVMNFLFVVLWAFAMPFSRFQHMASCLCTIWTSIIIVCKMLYQLTVVNPLEYSSNCTQPLLNNTSLLEDEILNSTLYREPIDPANWFGIRKGYPNLGYIQNHLQILLLLVFEAIVYRHQEFYRKQYQLEKPHIQAVFPDVYREHLDQGLFSCIKYFINYSFYKFGLEICFLLTVNVIGQRMNFMVIFHGCWLIVILTRRQRVNIAKLWPKYCVFLIFFLLWQYMICLGIPPVLCIDYPWRWDTFVPVNSALIKWLYLPDFYTTPKSVNLISDFVLLIGVAQQWKVFINEKKEEWYTMAGENVEEYEAPKDRPNPVPNFINCQSYLDMVKVAVFRYLFWFVLTVVFITGATRISVFGLGYLMSCFYLLLSGTTLLQKPMKAKLVLWDCLILYNITVILSKNMLSLLSCVFVQQMQTNFCWVIQLFSLVCTVKGYYNPQDMDKDRDCSLPVEEAGIIWDSICFLFLLLQRRVFLSCYFRHVVLDLRASSLQTSRGFELFMASIRKNVFFHQESERRSLNQLRKQMERIRAKQERYHEGRRQTDSSQVKEGEPGSKKGSSRQKIHWSRPWLDHASVLHSGDYFLFESDSEEDEESLLEQDQRPPKQSAFQLAYQAWVTSAKSALKERKSRQKERSGKVEMVTVENNEAGGGTESHTQEPREKGEDKDLNEERGGSNVVQRILDVLKFLWVLLLAMVDGLINWLGTLTKQHVDMSTVLWVERYIITQKLSKGEDINRRDLEKVYETGQDVREPRISSPPTDMSTSIASSGCEEEELICSTAECTENITQLSTGYNTRSNSLDSIRGFPEIKPSHRSRTASELLLSRPLDFPELSESQHFFQSQSRILRLLLALYNFVAAHSELLCYFIIILNNMVSASLISLFLPILIFLWGMLSIHKPSKRFWMTAIIFTEVMVVIKYLFQFGFFPWNNYTILVRNEGKPFFPPRVLGLEKSDSYIKYDLIQLLMLFFHRSLLMSYGLWDRKDLDLEERTPGKDKGSKTEIQASQDLEPAGGSSSSLRQRKRKESQGSVQERDSLKEKSLDVQKKKKGRVALRVKIMGMKMQQFFACIVRNIYQPVKAFFHDILHTKNRAATDVYAFMFLADVVDFVIIVFGFWAFGKHSAAADIASSLSDDQVPEAFLVMVLIQFSTMVIDRALYLRKTVLGKLIFQVILVIGIHVWMFFILPAVTERMFNQNTVAQLWYFVKCIYFALSAYQIRCGYPTRILGNFLTKKYNHLNLFLFQGFRLVPFLVELRAVMDWVWTDTTLSLSNWMCVEDIYANIFIIKCSRETEKKYPQPKGQKKKKIVKYGMGGLIIVFLICIIWFPLLFMSLVRSVVGVVNHPIDVTVTFKLGGYEPLFTMSAQQQSIQPFTPQQYEALTYEFERQPTAMQFITLYSYEDIVTARIEGSSGSVWGISPPSREQMRKELQNGSSDITLRFTWDFQRDLAKGGTVEHTSEKHTKDLEPGSQERMELADLLQGVRDTPVSIQHLFPKYIRAPNGPEANPVKQLQPDEEESYLDVEVQLNRMRVAEGNSTSSFVEWWVITLKDCEGEDCNILPMVIFNDKVSPPSLGFLAGYGIMGLYVSIVLVIGKFVRGFFSEISHSIMFEELPCVDRILKLCQDIYVVRERGELELEEELYAKLIFLYRSPETMIKWTVDKD

Target ORF information:

RefSeq Version	XM_002933675.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis piezo-type mechanosensitive ion channel component 1 (piezo1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002933675.4

ORF Insert Sequence:

ATGGAACTGC AAGTCCTCTG TGGGGTCCTG TTTTGGCTCC TTCTGCCCGG CTCCCTCTTG 
GCAGCCTGCT TATTCCGTTA CAACTCCTTG TCACTGGTGT ACCTGTTTTA TCTTCTGCTT 
CTGCCATGGT TTGCTGGACC AAATCCCTGC AGCATGAGAG GTCACACAGG ACGTCTGATG 
AAAGCGTTAC TTGCTACCAG CATCCTCTTC CTCTGTGCTC ATCTCATTTT CCAGATCTGC 
CTGTACACAA TACCTGAACT GGATGACCTG CTAGGGCACA ACTGCAGCAG CTGGGATGTG 
TTGGCTCGTC ACGTAGGTAT AAACAGGCTG GATCTTTCTG ATATCCCCAA CATGGTGCGA 
CAAGTGTCCC CAGATTTTGG CATGTTTGTC GTGTCAGCAT TGTGCCTTGG CTTGTGTAAT 
CGCCTAGTGA CCAAGAAGAG ACGACAGAGG TCTTCAGGCT ATTTTGAGCA GGAAGAAGAG 
AGGACTGAAC TTAACCTAAA ATATGAAAGT AGAGAGTGTG ATACAGAGTT TGAGGTGGGG 
GGAGACTTAC ATTCTGTTCC ACGAGACCGC CTTCTTGGGT CCTGGCTTAG AAAGGCAGCA 
TACAGGCTTC TGGAAACAGT GGAGAAATCA CTTGCTGTCT TACTCTTGGC TCTTGCAGGT 
ATCACTGATC CCTCTGCTTT CTCAGCCCCA TATTATGTGG TTTTCATTGG TCTCTGCACA 
TGGTGGGCCT GCCATTTTCC CATTAGCCAA CTAGGCTTTA ATACTATCTG TGTCATTGTT 
GGGTTCTTCA CTGGAGGTCA CATGATTTGC CTTTATATTT ACCAGACACC TTTTATCCAA 
CAAATGTTTC ATCCTGAACA GTTATGGGCA AGGTTGTTTG GTCTGCATAA CATCATCAGT 
CACTCCAACA TTACAGACTG TACCCATGAC TCTCTGAATC AAAACCTTGA CTGGCCTGTG 
TATGTTCATC CCGGAATCTT ATTACTGCTG TACTACTCCC TATTATCAAT ACTGAAACTG 
AAGAGCTCTG ACAAGAAAAG GATTTCAGGG TGTGACCTTC GGAAATCTCA GCAGGAGGAT 
ATGGAGTTGA CCGATATTGA GCCAGTTTCA AAGGAGCAAT ATAGTGCGGG AGAAGAGACA 
AAACCAATGC TTCAGAGGAT GAACACTGAA GAAGAGGCTA AACCCCAAGA CTGCACTGTT 
CATGTGATTA GAAGAAATTC TAATCAACTG CCAGAAGAAC TTGAAACCAA GGAGCCATCA 
CAATTTCACC TGATGATTCA TGTAATACTG AGCCAAAGCT ATATCTGTGC GCTTATTGCA 
ATGATGGTGT GGAGCATTAC ATATCACAGC TGGCTGACTT TTGTGCTGCT GCTCTGGGCA 
TGTTTAATCT GGATGGTGAG GAACAGGCGC CAGTTTGCAA TGCTGTGCTC TCCCTTTATT 
TTGATGTATG GAGTGACTCT CTGCAGCCTG CAGTATGTCT GGAGTATGGA CTTGAGAGAA 
GAGCTTCCGA CACAACTTGG TTTTATGAAG CTGTCACAGC TGGGACTGGT TCGCGCACCA 
TACCCCTGCC TTAACATGGG AGCACTGCTA CTTTACACGC TGACATTTTG GCTTCTGCTG 
CACCAATTTG TAACAGAAAA ATTTCTAAAA CGTAAGAAGT TTATGTCCCC ACTCCTGGAG 
GTCACAGTGG CAGATGCTGG CAAAGAAAAG AACCTGCTAA AACGTTTTGG GGAGCAAGTA 
AAAAGCTTCT ATGCAAAATA CTGGATCTAT GTGTGTGGAG GCATGTTTAT TGTGGTCAGC 
TTTGCTGGTA GGCTGGTAGT TTACAAAATA GTCTACATGC TTCTGTTCCT ACTATGCTTG 
GTGTTATTTC AGGTGTATTA CTCTTTGTGG AGGAAGCTGC TGACTTTTTT CTGGTGGTTA 
GTTGTAGCAT ACACAATGTT GGTTCTTATC ACCATCTACA CCTTTCAATT TGAAGAGTTT 
CCTGAGTACT GGAAAAACTT GACCAACCTT ACAGATGAGC AGTTGGGTGA TATTGGGCTT 
GAGAGGTTTA GTGTGTCGGA ACTTTTTACC AGCATCCTTA TCCCAGGCTT CTTCCTCCTT 
GCCTGCATCC TCCAGTTACA CTACTTTCAT CGCCCTTTTA TGCACATGAC CAACTTGGCA 
CATGCCTCAT CCCAAGGAAG TTTCCATAGC CACGAGAGTG AGCAGCCAAA GGAAGAAGAA 
GAGAAAGCTG GTGCATGCAG TGATGCTGCT GCTGATGAGG AGGAGGATGA GGAAGAATCT 
GCACATGGAG CTGATCTAAG TAAATGGGAC CTAGTTACAC AGCGCCTTTC TGTCCTGTCC 
TTTAAGTTCT CTGAGCTGCT GCACCGATTA CAGGTTTTCC TTTGGAGGCT TTTGGAGCTC 
CATGTGTTGA AACTGGTGGC TTTGTATAGC GTGTGGGTTG CCCTGCAGGA GGTATCTGTG 
ATGAATTTCC TTTTTGTGGT CCTATGGGCT TTTGCTATGC CTTTCTCCCG ATTCCAGCAC 
ATGGCTTCCT GTCTTTGCAC CATCTGGACC TCTATAATTA TTGTTTGCAA AATGCTTTAC 
CAGCTTACTG TTGTCAACCC TCTGGAGTAC TCTAGCAACT GTACTCAGCC TCTTCTAAAT 
AACACCAGTC TCCTAGAAGA CGAGATTTTG AACTCCACCC TTTATAGAGA ACCTATAGAT 
CCAGCAAACT GGTTTGGTAT CAGAAAAGGT TATCCAAACC TAGGTTACAT ACAGAATCAC 
CTGCAAATAC TCCTACTTTT GGTATTTGAG GCAATTGTTT ATCGGCATCA GGAGTTTTAC 
CGTAAACAAT ACCAGCTTGA GAAGCCACAC ATTCAGGCTG TATTTCCAGA TGTCTACAGA 
GAGCACTTGG ACCAAGGCTT GTTCAGCTGT ATCAAATATT TTATCAACTA TTCCTTTTAT 
AAGTTTGGTC TTGAGATCTG CTTCCTTCTT ACTGTGAATG TGATTGGTCA GAGGATGAAC 
TTTATGGTAA TCTTTCATGG CTGCTGGCTG ATAGTGATTC TTACTCGACG ACAGCGAGTC 
AACATTGCAA AATTATGGCC TAAATACTGT GTGTTCCTAA TCTTTTTCCT GTTGTGGCAG 
TACATGATAT GTCTGGGCAT TCCTCCGGTG CTTTGTATAG ATTATCCCTG GAGGTGGGAC 
ACATTTGTTC CTGTCAACTC TGCACTGATA AAGTGGTTAT ATCTTCCAGA TTTCTATACT 
ACACCTAAAT CTGTCAACCT TATCAGTGAT TTTGTTTTGC TGATTGGTGT GGCCCAGCAG 
TGGAAGGTGT TTATTAATGA GAAGAAGGAA GAATGGTACA CTATGGCAGG GGAAAATGTG 
GAAGAGTATG AAGCTCCAAA AGACAGGCCC AACCCTGTAC CTAACTTCAT CAATTGTCAG 
TCTTATCTGG ATATGGTGAA AGTAGCAGTG TTTCGGTACC TATTCTGGTT TGTCTTGACT 
GTGGTCTTTA TAACAGGGGC CACTCGCATC AGTGTTTTTG GCCTAGGATA TTTGATGTCC 
TGTTTCTACC TTCTTCTGTC TGGCACAACC CTTCTGCAAA AGCCAATGAA AGCCAAGCTG 
GTGCTTTGGG ACTGTTTGAT CCTCTACAAC ATCACTGTTA TACTGTCCAA GAACATGCTG 
TCGCTCCTGT CTTGTGTATT TGTTCAACAA ATGCAAACCA ACTTTTGCTG GGTGATCCAG 
CTCTTCAGCC TAGTTTGCAC AGTTAAGGGT TACTACAATC CCCAAGATAT GGATAAAGAC 
CGGGATTGCT CTCTGCCAGT GGAAGAGGCC GGAATCATTT GGGATAGCAT CTGCTTCTTA 
TTTCTCCTTC TCCAACGAAG AGTTTTTTTG AGTTGCTACT TTCGGCATGT TGTTCTTGAC 
CTTCGTGCCT CCTCCCTTCA AACATCAAGA GGTTTTGAGC TCTTCATGGC CAGCATCAGA 
AAGAATGTGT TTTTCCACCA AGAATCTGAG AGACGATCTC TTAACCAGCT CAGGAAGCAA 
ATGGAGAGAA TCCGAGCAAA GCAAGAACGC TATCATGAAG GCAGAAGACA AACTGATAGC 
AGTCAAGTAA AGGAAGGTGA GCCTGGCAGC AAGAAAGGGT CCAGTAGGCA AAAGATTCAT 
TGGAGCCGCC CATGGTTAGA CCACGCCTCA GTGCTGCATT CAGGAGATTA TTTTCTGTTC 
GAATCAGACA GTGAAGAGGA TGAAGAGTCT TTATTGGAGC AGGATCAGAG GCCACCCAAG 
CAGAGTGCTT TTCAGCTTGC ATACCAGGCA TGGGTTACAA GTGCCAAATC TGCGCTAAAG 
GAAAGAAAGA GTCGGCAGAA AGAGAGATCT GGAAAGGTAG AAATGGTCAC GGTAGAGAAT 
AATGAAGCAG GAGGGGGGAC AGAGTCTCAT ACCCAGGAAC CAAGGGAGAA AGGAGAGGAT 
AAAGATCTGA ATGAGGAGAG AGGAGGAAGC AATGTGGTAC AGCGTATCCT TGACGTGTTG 
AAGTTTCTCT GGGTTCTTTT GCTTGCCATG GTGGATGGAC TGATCAATTG GCTAGGAACC 
CTTACAAAGC AGCATGTTGA TATGTCCACA GTACTGTGGG TAGAGAGATA TATCATAACC 
CAAAAACTGT CTAAGGGAGA AGATATCAAC CGCAGGGATT TGGAAAAGGT ATATGAGACA 
GGGCAGGATG TTAGGGAGCC TAGGATTTCA TCTCCTCCAA CTGATATGTC AACTAGCATT 
GCTTCAAGTG GCTGTGAGGA AGAGGAGCTT ATCTGCAGCA CAGCAGAGTG TACGGAAAAT 
ATAACACAAC TCAGCACCGG GTACAACACA CGAAGTAACA GCTTGGACTC TATTAGGGGG 
TTTCCGGAAA TCAAGCCAAG TCATCGTTCT CGTACTGCTA GTGAATTGCT GCTTTCCAGG 
CCACTTGATT TCCCAGAGCT CTCCGAGTCT CAGCACTTCT TTCAGTCTCA GAGTCGTATT 
CTGAGGCTCC TGTTGGCCCT CTATAACTTT GTGGCTGCTC ACTCTGAGCT GCTTTGTTAC 
TTCATCATCA TTCTCAACAA CATGGTGAGC GCTTCCCTGA TATCACTGTT CCTTCCAATT 
CTCATCTTCC TGTGGGGTAT GCTGTCCATC CACAAACCCA GTAAGCGCTT CTGGATGACT 
GCTATCATTT TTACTGAGGT TATGGTAGTA ATAAAATACC TCTTCCAGTT TGGTTTCTTC 
CCATGGAATA ATTATACCAT TCTGGTACGC AACGAAGGGA AACCATTTTT TCCTCCACGT 
GTCCTGGGAC TTGAGAAGAG TGACTCGTAC ATCAAATATG ATCTGATCCA GCTCTTGATG 
CTCTTTTTCC ATCGCTCACT GCTCATGAGT TATGGTCTTT GGGATCGTAA AGACTTAGAC 
CTTGAAGAGA GAACACCCGG GAAGGATAAA GGGAGCAAAA CAGAGATTCA GGCATCCCAG 
GATCTGGAGC CAGCAGGAGG CAGTAGCTCT TCCCTGCGTC AGCGCAAAAG AAAAGAATCC 
CAGGGCAGTG TGCAAGAAAG AGATTCTTTA AAAGAGAAAT CACTAGATGT GCAGAAGAAG 
AAAAAAGGAA GAGTGGCTCT TAGGGTGAAG ATCATGGGAA TGAAAATGCA ACAGTTCTTT 
GCCTGCATAG TGCGGAATAT ATATCAGCCA GTGAAAGCTT TTTTCCATGA TATTCTACAT 
ACCAAGAACC GTGCTGCAAC AGATGTTTAT GCCTTCATGT TCCTCGCAGA TGTGGTTGAC 
TTTGTGATCA TCGTATTTGG GTTTTGGGCT TTTGGAAAAC ACTCTGCTGC TGCCGACATA 
GCATCATCTC TCTCTGATGA CCAAGTACCA GAAGCTTTCC TAGTCATGGT CCTCATACAG 
TTTTCCACCA TGGTGATAGA TCGTGCTCTA TACCTGCGGA AGACTGTGCT TGGAAAGCTC 
ATCTTTCAAG TTATTCTGGT GATTGGGATA CATGTCTGGA TGTTCTTTAT CCTACCAGCA 
GTCACAGAAA GGATGTTCAA TCAGAACACT GTGGCCCAGC TCTGGTATTT TGTTAAGTGC 
ATTTACTTTG CCCTGTCTGC CTACCAGATC CGCTGTGGGT ATCCTACCCG CATCCTGGGC 
AACTTCCTTA CCAAGAAATA CAACCACCTC AACCTATTCC TATTTCAGGG GTTTCGCTTG 
GTTCCGTTTC TAGTAGAGCT CAGGGCTGTA ATGGACTGGG TTTGGACTGA TACCACCTTG 
TCCTTGTCCA ACTGGATGTG CGTGGAAGAC ATTTATGCTA ATATCTTCAT TATCAAATGC 
AGCCGAGAGA CTGAAAAGAA ATACCCTCAG CCTAAAGGAC AGAAAAAAAA GAAGATAGTG 
AAATATGGAA TGGGTGGTTT GATCATAGTC TTCCTTATCT GTATAATCTG GTTTCCCCTT 
CTCTTCATGT CGTTGGTGCG CTCAGTGGTG GGAGTCGTCA ACCACCCTAT CGATGTCACG 
GTTACTTTTA AACTCGGGGG TTATGAGCCT TTATTCACGA TGAGTGCACA GCAACAGTCC 
ATCCAGCCAT TCACCCCACA GCAATATGAA GCGCTTACCT ATGAGTTTGA GAGACAGCCG 
ACTGCCATGC AGTTCATTAC TTTGTATAGT TATGAGGACA TAGTGACTGC GCGAATTGAG 
GGAAGTTCAG GATCTGTATG GGGAATCAGC CCTCCAAGCC GTGAGCAGAT GAGAAAGGAG 
CTGCAAAATG GATCCTCTGA CATTACACTA CGCTTCACCT GGGATTTTCA GAGGGATCTG 
GCAAAAGGTG GCACTGTGGA GCACACATCT GAGAAACACA CTAAGGACTT GGAGCCTGGG 
AGCCAGGAGA GGATGGAGCT GGCAGATCTT CTTCAAGGAG TACGAGACAC CCCTGTCTCT 
ATCCAGCACT TGTTTCCAAA GTACATCCGC GCTCCTAATG GCCCAGAAGC AAATCCAGTG 
AAACAGCTCC AGCCAGATGA AGAAGAAAGT TACCTGGATG TGGAGGTGCA GCTAAATCGG 
ATGCGAGTTG CAGAGGGAAA TAGCACCAGC AGTTTTGTGG AGTGGTGGGT TATTACTCTA 
AAAGACTGCG AGGGAGAAGA CTGCAACATA CTACCTATGG TGATTTTTAA CGATAAAGTC 
AGCCCTCCTA GCTTAGGATT CCTGGCTGGA TATGGGATAA TGGGTTTGTA TGTATCTATT 
GTCCTGGTCA TTGGGAAGTT TGTGCGCGGC TTCTTCAGTG AAATCTCTCA CTCCATCATG 
TTTGAGGAGC TGCCTTGTGT GGATCGGATT CTCAAGCTGT GCCAGGATAT CTATGTGGTG 
CGGGAGCGTG GGGAATTGGA ACTGGAAGAG GAACTCTATG CAAAACTCAT CTTCCTGTAT 
CGCTCACCAG AAACCATGAT CAAGTGGACT GTAGATAAGG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

piezo1 ( XM_012962280.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817734.1 Xenopus tropicalis piezo-type mechanosensitive ion channel component 1 (piezo1), transcript variant X2, mRNA.

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CloneID	OXa34346
Clone ID Related Accession (Same CDS sequence)	XM_012962280.1
Accession Version	XM_012962280.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	piezo-type mechanosensitive ion channel component 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACTGC AAGTCCTCTG TGGGGTCCTG TTTTGGCTCC TTCTGCCCGG CTCCCTCTTG 
GCAGCCTGCT TATTCCGTTA CAACTCCTTG TCACTGGTGT ACCTGTTTTA TCTTCTGCTT 
CTGCCATGGT TTGCTGGACC AAATCCCTGC AGCATGAGAG GTCACACAGG ACGTCTGATG 
AAAGCGTTAC TTGCTACCAG CATCCTCTTC CTCTGTGCTC ATCTCATTTT CCAGATCTGC 
CTGTACACAA TACCTGAACT GGATGACCTG CTAGGGCACA ACTGCAGCAG CTGGGATGTG 
TTGGCTCGTC ACGTAGGTAT AAACAGGCTG GATCTTTCTG ATATCCCCAA CATGGTGCGA 
CAAGTGTCCC CAGATTTTGG CATGTTTGTC GTGTCAGCAT TGTGCCTTGG CTTGTGTAAT 
CGCCTAGTGA CCAAGAAGAG ACGACAGAGG TCTTCAGGCT ATTTTGAGCA GGAAGAAGAG 
AGGACTGAAC TTAACCTAAA ATATGAAAGT AGAGAGTGTG ATACAGAGTT TGAGGTGGGG 
GGAGACTTAC ATTCTGTTCC ACGAGACCGC CTTCTTGGGT CCTGGCTTAG AAAGGCAGCA 
TACAGGCTTC TGGAAACAGT GGAGAAATCA CTTGCTGTCT TACTCTTGGC TCTTGCAGGT 
ATCACTGATC CCTCTGCTTT CTCAGCCCCA TATTATGTGG TTTTCATTGG TCTCTGCACA 
TGGTGGGCCT GCCATTTTCC CATTAGCCAA CTAGGCTTTA ATACTATCTG TGTCATTGTT 
GGGTTCTTCA CTGGAGGTCA CATGATTTGC CTTTATATTT ACCAGACACC TTTTATCCAA 
CAAATGTTTC ATCCTGAACA GTTATGGGCA AGGTTGTTTG GTCTGCATAA CATCATCAGT 
CACTCCAACA TTACAGACTG TACCCATGAC TCTCTGAATC AAAACCTTGA CTGGCCTGTG 
TATGTTCATC CCGGAATCTT ATTACTGCTG TACTACTCCC TATTATCAAT ACTGAAACTG 
AAGAGCTCTG ACAAGAAAAG GATTTCAGGG TGTGACCTTC GGAAATCTCA GCAGGAGGAT 
ATGGAGTTGA CCGATATTGA GCCAGTTTCA AAGGAGCAAT ATAGTGCGGG AGAAGAGACA 
AAACCAATGC TTCAGAGGAT GAACACTGAA GAAGAGGCTA AACCCCAAGA CTGCACTGTT 
CATGTGATTA GAAGAAATTC TAATCAACTG CCAGAACTTG AAACCAAGGA GCCATCACAA 
TTTCACCTGA TGATTCATGT AATACTGAGC CAAAGCTATA TCTGTGCGCT TATTGCAATG 
ATGGTGTGGA GCATTACATA TCACAGCTGG CTGACTTTTG TGCTGCTGCT CTGGGCATGT 
TTAATCTGGA TGGTGAGGAA CAGGCGCCAG TTTGCAATGC TGTGCTCTCC CTTTATTTTG 
ATGTATGGAG TGACTCTCTG CAGCCTGCAG TATGTCTGGA GTATGGACTT GAGAGAAGAG 
CTTCCGACAC AACTTGGTTT TATGAAGCTG TCACAGCTGG GACTGGTTCG CGCACCATAC 
CCCTGCCTTA ACATGGGAGC ACTGCTACTT TACACGCTGA CATTTTGGCT TCTGCTGCAC 
CAATTTGTAA CAGAAAAATT TCTAAAACGT AAGAAGTTTA TGTCCCCACT CCTGGAGGTC 
ACAGTGGCAG ATGCTGGCAA AGAAAAGAAC CTGCTAAAAC GTTTTGGGGA GCAAGTAAAA 
AGCTTCTATG CAAAATACTG GATCTATGTG TGTGGAGGCA TGTTTATTGT GGTCAGCTTT 
GCTGGTAGGC TGGTAGTTTA CAAAATAGTC TACATGCTTC TGTTCCTACT ATGCTTGGTG 
TTATTTCAGG TGTATTACTC TTTGTGGAGG AAGCTGCTGA CTTTTTTCTG GTGGTTAGTT 
GTAGCATACA CAATGTTGGT TCTTATCACC ATCTACACCT TTCAATTTGA AGAGTTTCCT 
GAGTACTGGA AAAACTTGAC CAACCTTACA GATGAGCAGT TGGGTGATAT TGGGCTTGAG 
AGGTTTAGTG TGTCGGAACT TTTTACCAGC ATCCTTATCC CAGGCTTCTT CCTCCTTGCC 
TGCATCCTCC AGTTACACTA CTTTCATCGC CCTTTTATGC ACATGACCAA CTTGGCACAT 
GCCTCATCCC AAGGAAGTTT CCATAGCCAC GAGAGTGAGC AGCCAAAGGA AGAAGAAGAG 
AAAGCTGGTG CATGCAGTGA TGCTGCTGCT GATGAGGAGG AGGATGAGGA AGAATCTGCA 
CATGGAGCTG ATCTAAGTAA ATGGGACCTA GTTACACAGC GCCTTTCTGT CCTGTCCTTT 
AAGTTCTCTG AGCTGCTGCA CCGATTACAG GTTTTCCTTT GGAGGCTTTT GGAGCTCCAT 
GTGTTGAAAC TGGTGGCTTT GTATAGCGTG TGGGTTGCCC TGCAGGAGGT ATCTGTGATG 
AATTTCCTTT TTGTGGTCCT ATGGGCTTTT GCTATGCCTT TCTCCCGATT CCAGCACATG 
GCTTCCTGTC TTTGCACCAT CTGGACCTCT ATAATTATTG TTTGCAAAAT GCTTTACCAG 
CTTACTGTTG TCAACCCTCT GGAGTACTCT AGCAACTGTA CTCAGCCTCT TCTAAATAAC 
ACCAGTCTCC TAGAAGACGA GATTTTGAAC TCCACCCTTT ATAGAGAACC TATAGATCCA 
GCAAACTGGT TTGGTATCAG AAAAGGTTAT CCAAACCTAG GTTACATACA GAATCACCTG 
CAAATACTCC TACTTTTGGT ATTTGAGGCA ATTGTTTATC GGCATCAGGA GTTTTACCGT 
AAACAATACC AGCTTGAGAA GCCACACATT CAGGCTGTAT TTCCAGATGT CTACAGAGAG 
CACTTGGACC AAGGCTTGTT CAGCTGTATC AAATATTTTA TCAACTATTC CTTTTATAAG 
TTTGGTCTTG AGATCTGCTT CCTTCTTACT GTGAATGTGA TTGGTCAGAG GATGAACTTT 
ATGGTAATCT TTCATGGCTG CTGGCTGATA GTGATTCTTA CTCGACGACA GCGAGTCAAC 
ATTGCAAAAT TATGGCCTAA ATACTGTGTG TTCCTAATCT TTTTCCTGTT GTGGCAGTAC 
ATGATATGTC TGGGCATTCC TCCGGTGCTT TGTATAGATT ATCCCTGGAG GTGGGACACA 
TTTGTTCCTG TCAACTCTGC ACTGATAAAG TGGTTATATC TTCCAGATTT CTATACTACA 
CCTAAATCTG TCAACCTTAT CAGTGATTTT GTTTTGCTGA TTGGTGTGGC CCAGCAGTGG 
AAGGTGTTTA TTAATGAGAA GAAGGAAGAA TGGTACACTA TGGCAGGGGA AAATGTGGAA 
GAGTATGAAG CTCCAAAAGA CAGGCCCAAC CCTGTACCTA ACTTCATCAA TTGTCAGTCT 
TATCTGGATA TGGTGAAAGT AGCAGTGTTT CGGTACCTAT TCTGGTTTGT CTTGACTGTG 
GTCTTTATAA CAGGGGCCAC TCGCATCAGT GTTTTTGGCC TAGGATATTT GATGTCCTGT 
TTCTACCTTC TTCTGTCTGG CACAACCCTT CTGCAAAAGC CAATGAAAGC CAAGCTGGTG 
CTTTGGGACT GTTTGATCCT CTACAACATC ACTGTTATAC TGTCCAAGAA CATGCTGTCG 
CTCCTGTCTT GTGTATTTGT TCAACAAATG CAAACCAACT TTTGCTGGGT GATCCAGCTC 
TTCAGCCTAG TTTGCACAGT TAAGGGTTAC TACAATCCCC AAGATATGGA TAAAGACCGG 
GATTGCTCTC TGCCAGTGGA AGAGGCCGGA ATCATTTGGG ATAGCATCTG CTTCTTATTT 
CTCCTTCTCC AACGAAGAGT TTTTTTGAGT TGCTACTTTC GGCATGTTGT TCTTGACCTT 
CGTGCCTCCT CCCTTCAAAC ATCAAGAGGT TTTGAGCTCT TCATGGCCAG CATCAGAAAG 
AATGTGTTTT TCCACCAAGA ATCTGAGAGA CGATCTCTTA ACCAGCTCAG GAAGCAAATG 
GAGAGAATCC GAGCAAAGCA AGAACGCTAT CATGAAGGCA GAAGACAAAC TGATAGCAGT 
CAAGTAAAGG AAGGTGAGCC TGGCAGCAAG AAAGGGTCCA GTAGGCAAAA GATTCATTGG 
AGCCGCCCAT GGTTAGACCA CGCCTCAGTG CTGCATTCAG GAGATTATTT TCTGTTCGAA 
TCAGACAGTG AAGAGGATGA AGAGTCTTTA TTGGAGCAGG ATCAGAGGCC ACCCAAGCAG 
AGTGCTTTTC AGCTTGCATA CCAGGCATGG GTTACAAGTG CCAAATCTGC GCTAAAGGAA 
AGAAAGAGTC GGCAGAAAGA GAGATCTGGA AAGGTAGAAA TGGTCACGGT AGAGAATAAT 
GAAGCAGGAG GGGGGACAGA GTCTCATACC CAGGAACCAA GGGAGAAAGG AGAGGATAAA 
GATCTGAATG AGGAGAGAGG AGGAAGCAAT GTGGTACAGC GTATCCTTGA CGTGTTGAAG 
TTTCTCTGGG TTCTTTTGCT TGCCATGGTG GATGGACTGA TCAATTGGCT AGGAACCCTT 
ACAAAGCAGC ATGTTGATAT GTCCACAGTA CTGTGGGTAG AGAGATATAT CATAACCCAA 
AAACTGTCTA AGGGAGAAGA TATCAACCGC AGGGATTTGG AAAAGGTATA TGAGACAGGG 
CAGGATGTTA GGGAGCCTAG GATTTCATCT CCTCCAACTG ATATGTCAAC TAGCATTGCT 
TCAAGTGGCT GTGAGGAAGA GGAGCTTATC TGCAGCACAG CAGAGTGTAC GGAAAATATA 
ACACAACTCA GCACCGGGTA CAACACACGA AGTAACAGCT TGGACTCTAT TAGGGGGTTT 
CCGGAAATCA AGCCAAGTCA TCGTTCTCGT ACTGCTAGTG AATTGCTGCT TTCCAGGCCA 
CTTGATTTCC CAGAGCTCTC CGAGTCTCAG CACTTCTTTC AGTCTCAGAG TCGTATTCTG 
AGGCTCCTGT TGGCCCTCTA TAACTTTGTG GCTGCTCACT CTGAGCTGCT TTGTTACTTC 
ATCATCATTC TCAACAACAT GGTGAGCGCT TCCCTGATAT CACTGTTCCT TCCAATTCTC 
ATCTTCCTGT GGGGTATGCT GTCCATCCAC AAACCCAGTA AGCGCTTCTG GATGACTGCT 
ATCATTTTTA CTGAGGTTAT GGTAGTAATA AAATACCTCT TCCAGTTTGG TTTCTTCCCA 
TGGAATAATT ATACCATTCT GGTACGCAAC GAAGGGAAAC CATTTTTTCC TCCACGTGTC 
CTGGGACTTG AGAAGAGTGA CTCGTACATC AAATATGATC TGATCCAGCT CTTGATGCTC 
TTTTTCCATC GCTCACTGCT CATGAGTTAT GGTCTTTGGG ATCGTAAAGA CTTAGACCTT 
GAAGAGAGAA CACCCGGGAA GGATAAAGGG AGCAAAACAG AGATTCAGGC ATCCCAGGAT 
CTGGAGCCAG CAGGAGGCAG TAGCTCTTCC CTGCGTCAGC GCAAAAGAAA AGAATCCCAG 
GGCAGTGTGC AAGAAAGAGA TTCTTTAAAA GAGAAATCAC TAGATGTGCA GAAGAAGAAA 
AAAGGAAGAG TGGCTCTTAG GGTGAAGATC ATGGGAATGA AAATGCAACA GTTCTTTGCC 
TGCATAGTGC GGAATATATA TCAGCCAGTG AAAGCTTTTT TCCATGATAT TCTACATACC 
AAGAACCGTG CTGCAACAGA TGTTTATGCC TTCATGTTCC TCGCAGATGT GGTTGACTTT 
GTGATCATCG TATTTGGGTT TTGGGCTTTT GGAAAACACT CTGCTGCTGC CGACATAGCA 
TCATCTCTCT CTGATGACCA AGTACCAGAA GCTTTCCTAG TCATGGTCCT CATACAGTTT 
TCCACCATGG TGATAGATCG TGCTCTATAC CTGCGGAAGA CTGTGCTTGG AAAGCTCATC 
TTTCAAGTTA TTCTGGTGAT TGGGATACAT GTCTGGATGT TCTTTATCCT ACCAGCAGTC 
ACAGAAAGGA TGTTCAATCA GAACACTGTG GCCCAGCTCT GGTATTTTGT TAAGTGCATT 
TACTTTGCCC TGTCTGCCTA CCAGATCCGC TGTGGGTATC CTACCCGCAT CCTGGGCAAC 
TTCCTTACCA AGAAATACAA CCACCTCAAC CTATTCCTAT TTCAGGGGTT TCGCTTGGTT 
CCGTTTCTAG TAGAGCTCAG GGCTGTAATG GACTGGGTTT GGACTGATAC CACCTTGTCC 
TTGTCCAACT GGATGTGCGT GGAAGACATT TATGCTAATA TCTTCATTAT CAAATGCAGC 
CGAGAGACTG AAAAGAAATA CCCTCAGCCT AAAGGACAGA AAAAAAAGAA GATAGTGAAA 
TATGGAATGG GTGGTTTGAT CATAGTCTTC CTTATCTGTA TAATCTGGTT TCCCCTTCTC 
TTCATGTCGT TGGTGCGCTC AGTGGTGGGA GTCGTCAACC ACCCTATCGA TGTCACGGTT 
ACTTTTAAAC TCGGGGGTTA TGAGCCTTTA TTCACGATGA GTGCACAGCA ACAGTCCATC 
CAGCCATTCA CCCCACAGCA ATATGAAGCG CTTACCTATG AGTTTGAGAG ACAGCCGACT 
GCCATGCAGT TCATTACTTT GTATAGTTAT GAGGACATAG TGACTGCGCG AATTGAGGGA 
AGTTCAGGAT CTGTATGGGG AATCAGCCCT CCAAGCCGTG AGCAGATGAG AAAGGAGCTG 
CAAAATGGAT CCTCTGACAT TACACTACGC TTCACCTGGG ATTTTCAGAG GGATCTGGCA 
AAAGGTGGCA CTGTGGAGCA CACATCTGAG AAACACACTA AGGACTTGGA GCCTGGGAGC 
CAGGAGAGGA TGGAGCTGGC AGATCTTCTT CAAGGAGTAC GAGACACCCC TGTCTCTATC 
CAGCACTTGT TTCCAAAGTA CATCCGCGCT CCTAATGGCC CAGAAGCAAA TCCAGTGAAA 
CAGCTCCAGC CAGATGAAGA AGAAAGTTAC CTGGATGTGG AGGTGCAGCT AAATCGGATG 
CGAGTTGCAG AGGGAAATAG CACCAGCAGT TTTGTGGAGT GGTGGGTTAT TACTCTAAAA 
GACTGCGAGG GAGAAGACTG CAACATACTA CCTATGGTGA TTTTTAACGA TAAAGTCAGC 
CCTCCTAGCT TAGGATTCCT GGCTGGATAT GGGATAATGG GTTTGTATGT ATCTATTGTC 
CTGGTCATTG GGAAGTTTGT GCGCGGCTTC TTCAGTGAAA TCTCTCACTC CATCATGTTT 
GAGGAGCTGC CTTGTGTGGA TCGGATTCTC AAGCTGTGCC AGGATATCTA TGTGGTGCGG 
GAGCGTGGGG AATTGGAACT GGAAGAGGAA CTCTATGCAA AACTCATCTT CCTGTATCGC 
TCACCAGAAA CCATGATCAA GTGGACTGTA GATAAGGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817734.1
CDS	1201..8622
Translation	MELQVLCGVLFWLLLPGSLLAACLFRYNSLSLVYLFYLLLLPWFAGPNPCSMRGHTGRLMKALLATSILFLCAHLIFQICLYTIPELDDLLGHNCSSWDVLARHVGINRLDLSDIPNMVRQVSPDFGMFVVSALCLGLCNRLVTKKRRQRSSGYFEQEEERTELNLKYESRECDTEFEVGGDLHSVPRDRLLGSWLRKAAYRLLETVEKSLAVLLLALAGITDPSAFSAPYYVVFIGLCTWWACHFPISQLGFNTICVIVGFFTGGHMICLYIYQTPFIQQMFHPEQLWARLFGLHNIISHSNITDCTHDSLNQNLDWPVYVHPGILLLLYYSLLSILKLKSSDKKRISGCDLRKSQQEDMELTDIEPVSKEQYSAGEETKPMLQRMNTEEEAKPQDCTVHVIRRNSNQLPELETKEPSQFHLMIHVILSQSYICALIAMMVWSITYHSWLTFVLLLWACLIWMVRNRRQFAMLCSPFILMYGVTLCSLQYVWSMDLREELPTQLGFMKLSQLGLVRAPYPCLNMGALLLYTLTFWLLLHQFVTEKFLKRKKFMSPLLEVTVADAGKEKNLLKRFGEQVKSFYAKYWIYVCGGMFIVVSFAGRLVVYKIVYMLLFLLCLVLFQVYYSLWRKLLTFFWWLVVAYTMLVLITIYTFQFEEFPEYWKNLTNLTDEQLGDIGLERFSVSELFTSILIPGFFLLACILQLHYFHRPFMHMTNLAHASSQGSFHSHESEQPKEEEEKAGACSDAAADEEEDEEESAHGADLSKWDLVTQRLSVLSFKFSELLHRLQVFLWRLLELHVLKLVALYSVWVALQEVSVMNFLFVVLWAFAMPFSRFQHMASCLCTIWTSIIIVCKMLYQLTVVNPLEYSSNCTQPLLNNTSLLEDEILNSTLYREPIDPANWFGIRKGYPNLGYIQNHLQILLLLVFEAIVYRHQEFYRKQYQLEKPHIQAVFPDVYREHLDQGLFSCIKYFINYSFYKFGLEICFLLTVNVIGQRMNFMVIFHGCWLIVILTRRQRVNIAKLWPKYCVFLIFFLLWQYMICLGIPPVLCIDYPWRWDTFVPVNSALIKWLYLPDFYTTPKSVNLISDFVLLIGVAQQWKVFINEKKEEWYTMAGENVEEYEAPKDRPNPVPNFINCQSYLDMVKVAVFRYLFWFVLTVVFITGATRISVFGLGYLMSCFYLLLSGTTLLQKPMKAKLVLWDCLILYNITVILSKNMLSLLSCVFVQQMQTNFCWVIQLFSLVCTVKGYYNPQDMDKDRDCSLPVEEAGIIWDSICFLFLLLQRRVFLSCYFRHVVLDLRASSLQTSRGFELFMASIRKNVFFHQESERRSLNQLRKQMERIRAKQERYHEGRRQTDSSQVKEGEPGSKKGSSRQKIHWSRPWLDHASVLHSGDYFLFESDSEEDEESLLEQDQRPPKQSAFQLAYQAWVTSAKSALKERKSRQKERSGKVEMVTVENNEAGGGTESHTQEPREKGEDKDLNEERGGSNVVQRILDVLKFLWVLLLAMVDGLINWLGTLTKQHVDMSTVLWVERYIITQKLSKGEDINRRDLEKVYETGQDVREPRISSPPTDMSTSIASSGCEEEELICSTAECTENITQLSTGYNTRSNSLDSIRGFPEIKPSHRSRTASELLLSRPLDFPELSESQHFFQSQSRILRLLLALYNFVAAHSELLCYFIIILNNMVSASLISLFLPILIFLWGMLSIHKPSKRFWMTAIIFTEVMVVIKYLFQFGFFPWNNYTILVRNEGKPFFPPRVLGLEKSDSYIKYDLIQLLMLFFHRSLLMSYGLWDRKDLDLEERTPGKDKGSKTEIQASQDLEPAGGSSSSLRQRKRKESQGSVQERDSLKEKSLDVQKKKKGRVALRVKIMGMKMQQFFACIVRNIYQPVKAFFHDILHTKNRAATDVYAFMFLADVVDFVIIVFGFWAFGKHSAAADIASSLSDDQVPEAFLVMVLIQFSTMVIDRALYLRKTVLGKLIFQVILVIGIHVWMFFILPAVTERMFNQNTVAQLWYFVKCIYFALSAYQIRCGYPTRILGNFLTKKYNHLNLFLFQGFRLVPFLVELRAVMDWVWTDTTLSLSNWMCVEDIYANIFIIKCSRETEKKYPQPKGQKKKKIVKYGMGGLIIVFLICIIWFPLLFMSLVRSVVGVVNHPIDVTVTFKLGGYEPLFTMSAQQQSIQPFTPQQYEALTYEFERQPTAMQFITLYSYEDIVTARIEGSSGSVWGISPPSREQMRKELQNGSSDITLRFTWDFQRDLAKGGTVEHTSEKHTKDLEPGSQERMELADLLQGVRDTPVSIQHLFPKYIRAPNGPEANPVKQLQPDEEESYLDVEVQLNRMRVAEGNSTSSFVEWWVITLKDCEGEDCNILPMVIFNDKVSPPSLGFLAGYGIMGLYVSIVLVIGKFVRGFFSEISHSIMFEELPCVDRILKLCQDIYVVRERGELELEEELYAKLIFLYRSPETMIKWTVDKD

Target ORF information:

RefSeq Version	XM_012962280.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis piezo-type mechanosensitive ion channel component 1 (piezo1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012962280.1

ORF Insert Sequence:

ATGGAACTGC AAGTCCTCTG TGGGGTCCTG TTTTGGCTCC TTCTGCCCGG CTCCCTCTTG 
GCAGCCTGCT TATTCCGTTA CAACTCCTTG TCACTGGTGT ACCTGTTTTA TCTTCTGCTT 
CTGCCATGGT TTGCTGGACC AAATCCCTGC AGCATGAGAG GTCACACAGG ACGTCTGATG 
AAAGCGTTAC TTGCTACCAG CATCCTCTTC CTCTGTGCTC ATCTCATTTT CCAGATCTGC 
CTGTACACAA TACCTGAACT GGATGACCTG CTAGGGCACA ACTGCAGCAG CTGGGATGTG 
TTGGCTCGTC ACGTAGGTAT AAACAGGCTG GATCTTTCTG ATATCCCCAA CATGGTGCGA 
CAAGTGTCCC CAGATTTTGG CATGTTTGTC GTGTCAGCAT TGTGCCTTGG CTTGTGTAAT 
CGCCTAGTGA CCAAGAAGAG ACGACAGAGG TCTTCAGGCT ATTTTGAGCA GGAAGAAGAG 
AGGACTGAAC TTAACCTAAA ATATGAAAGT AGAGAGTGTG ATACAGAGTT TGAGGTGGGG 
GGAGACTTAC ATTCTGTTCC ACGAGACCGC CTTCTTGGGT CCTGGCTTAG AAAGGCAGCA 
TACAGGCTTC TGGAAACAGT GGAGAAATCA CTTGCTGTCT TACTCTTGGC TCTTGCAGGT 
ATCACTGATC CCTCTGCTTT CTCAGCCCCA TATTATGTGG TTTTCATTGG TCTCTGCACA 
TGGTGGGCCT GCCATTTTCC CATTAGCCAA CTAGGCTTTA ATACTATCTG TGTCATTGTT 
GGGTTCTTCA CTGGAGGTCA CATGATTTGC CTTTATATTT ACCAGACACC TTTTATCCAA 
CAAATGTTTC ATCCTGAACA GTTATGGGCA AGGTTGTTTG GTCTGCATAA CATCATCAGT 
CACTCCAACA TTACAGACTG TACCCATGAC TCTCTGAATC AAAACCTTGA CTGGCCTGTG 
TATGTTCATC CCGGAATCTT ATTACTGCTG TACTACTCCC TATTATCAAT ACTGAAACTG 
AAGAGCTCTG ACAAGAAAAG GATTTCAGGG TGTGACCTTC GGAAATCTCA GCAGGAGGAT 
ATGGAGTTGA CCGATATTGA GCCAGTTTCA AAGGAGCAAT ATAGTGCGGG AGAAGAGACA 
AAACCAATGC TTCAGAGGAT GAACACTGAA GAAGAGGCTA AACCCCAAGA CTGCACTGTT 
CATGTGATTA GAAGAAATTC TAATCAACTG CCAGAACTTG AAACCAAGGA GCCATCACAA 
TTTCACCTGA TGATTCATGT AATACTGAGC CAAAGCTATA TCTGTGCGCT TATTGCAATG 
ATGGTGTGGA GCATTACATA TCACAGCTGG CTGACTTTTG TGCTGCTGCT CTGGGCATGT 
TTAATCTGGA TGGTGAGGAA CAGGCGCCAG TTTGCAATGC TGTGCTCTCC CTTTATTTTG 
ATGTATGGAG TGACTCTCTG CAGCCTGCAG TATGTCTGGA GTATGGACTT GAGAGAAGAG 
CTTCCGACAC AACTTGGTTT TATGAAGCTG TCACAGCTGG GACTGGTTCG CGCACCATAC 
CCCTGCCTTA ACATGGGAGC ACTGCTACTT TACACGCTGA CATTTTGGCT TCTGCTGCAC 
CAATTTGTAA CAGAAAAATT TCTAAAACGT AAGAAGTTTA TGTCCCCACT CCTGGAGGTC 
ACAGTGGCAG ATGCTGGCAA AGAAAAGAAC CTGCTAAAAC GTTTTGGGGA GCAAGTAAAA 
AGCTTCTATG CAAAATACTG GATCTATGTG TGTGGAGGCA TGTTTATTGT GGTCAGCTTT 
GCTGGTAGGC TGGTAGTTTA CAAAATAGTC TACATGCTTC TGTTCCTACT ATGCTTGGTG 
TTATTTCAGG TGTATTACTC TTTGTGGAGG AAGCTGCTGA CTTTTTTCTG GTGGTTAGTT 
GTAGCATACA CAATGTTGGT TCTTATCACC ATCTACACCT TTCAATTTGA AGAGTTTCCT 
GAGTACTGGA AAAACTTGAC CAACCTTACA GATGAGCAGT TGGGTGATAT TGGGCTTGAG 
AGGTTTAGTG TGTCGGAACT TTTTACCAGC ATCCTTATCC CAGGCTTCTT CCTCCTTGCC 
TGCATCCTCC AGTTACACTA CTTTCATCGC CCTTTTATGC ACATGACCAA CTTGGCACAT 
GCCTCATCCC AAGGAAGTTT CCATAGCCAC GAGAGTGAGC AGCCAAAGGA AGAAGAAGAG 
AAAGCTGGTG CATGCAGTGA TGCTGCTGCT GATGAGGAGG AGGATGAGGA AGAATCTGCA 
CATGGAGCTG ATCTAAGTAA ATGGGACCTA GTTACACAGC GCCTTTCTGT CCTGTCCTTT 
AAGTTCTCTG AGCTGCTGCA CCGATTACAG GTTTTCCTTT GGAGGCTTTT GGAGCTCCAT 
GTGTTGAAAC TGGTGGCTTT GTATAGCGTG TGGGTTGCCC TGCAGGAGGT ATCTGTGATG 
AATTTCCTTT TTGTGGTCCT ATGGGCTTTT GCTATGCCTT TCTCCCGATT CCAGCACATG 
GCTTCCTGTC TTTGCACCAT CTGGACCTCT ATAATTATTG TTTGCAAAAT GCTTTACCAG 
CTTACTGTTG TCAACCCTCT GGAGTACTCT AGCAACTGTA CTCAGCCTCT TCTAAATAAC 
ACCAGTCTCC TAGAAGACGA GATTTTGAAC TCCACCCTTT ATAGAGAACC TATAGATCCA 
GCAAACTGGT TTGGTATCAG AAAAGGTTAT CCAAACCTAG GTTACATACA GAATCACCTG 
CAAATACTCC TACTTTTGGT ATTTGAGGCA ATTGTTTATC GGCATCAGGA GTTTTACCGT 
AAACAATACC AGCTTGAGAA GCCACACATT CAGGCTGTAT TTCCAGATGT CTACAGAGAG 
CACTTGGACC AAGGCTTGTT CAGCTGTATC AAATATTTTA TCAACTATTC CTTTTATAAG 
TTTGGTCTTG AGATCTGCTT CCTTCTTACT GTGAATGTGA TTGGTCAGAG GATGAACTTT 
ATGGTAATCT TTCATGGCTG CTGGCTGATA GTGATTCTTA CTCGACGACA GCGAGTCAAC 
ATTGCAAAAT TATGGCCTAA ATACTGTGTG TTCCTAATCT TTTTCCTGTT GTGGCAGTAC 
ATGATATGTC TGGGCATTCC TCCGGTGCTT TGTATAGATT ATCCCTGGAG GTGGGACACA 
TTTGTTCCTG TCAACTCTGC ACTGATAAAG TGGTTATATC TTCCAGATTT CTATACTACA 
CCTAAATCTG TCAACCTTAT CAGTGATTTT GTTTTGCTGA TTGGTGTGGC CCAGCAGTGG 
AAGGTGTTTA TTAATGAGAA GAAGGAAGAA TGGTACACTA TGGCAGGGGA AAATGTGGAA 
GAGTATGAAG CTCCAAAAGA CAGGCCCAAC CCTGTACCTA ACTTCATCAA TTGTCAGTCT 
TATCTGGATA TGGTGAAAGT AGCAGTGTTT CGGTACCTAT TCTGGTTTGT CTTGACTGTG 
GTCTTTATAA CAGGGGCCAC TCGCATCAGT GTTTTTGGCC TAGGATATTT GATGTCCTGT 
TTCTACCTTC TTCTGTCTGG CACAACCCTT CTGCAAAAGC CAATGAAAGC CAAGCTGGTG 
CTTTGGGACT GTTTGATCCT CTACAACATC ACTGTTATAC TGTCCAAGAA CATGCTGTCG 
CTCCTGTCTT GTGTATTTGT TCAACAAATG CAAACCAACT TTTGCTGGGT GATCCAGCTC 
TTCAGCCTAG TTTGCACAGT TAAGGGTTAC TACAATCCCC AAGATATGGA TAAAGACCGG 
GATTGCTCTC TGCCAGTGGA AGAGGCCGGA ATCATTTGGG ATAGCATCTG CTTCTTATTT 
CTCCTTCTCC AACGAAGAGT TTTTTTGAGT TGCTACTTTC GGCATGTTGT TCTTGACCTT 
CGTGCCTCCT CCCTTCAAAC ATCAAGAGGT TTTGAGCTCT TCATGGCCAG CATCAGAAAG 
AATGTGTTTT TCCACCAAGA ATCTGAGAGA CGATCTCTTA ACCAGCTCAG GAAGCAAATG 
GAGAGAATCC GAGCAAAGCA AGAACGCTAT CATGAAGGCA GAAGACAAAC TGATAGCAGT 
CAAGTAAAGG AAGGTGAGCC TGGCAGCAAG AAAGGGTCCA GTAGGCAAAA GATTCATTGG 
AGCCGCCCAT GGTTAGACCA CGCCTCAGTG CTGCATTCAG GAGATTATTT TCTGTTCGAA 
TCAGACAGTG AAGAGGATGA AGAGTCTTTA TTGGAGCAGG ATCAGAGGCC ACCCAAGCAG 
AGTGCTTTTC AGCTTGCATA CCAGGCATGG GTTACAAGTG CCAAATCTGC GCTAAAGGAA 
AGAAAGAGTC GGCAGAAAGA GAGATCTGGA AAGGTAGAAA TGGTCACGGT AGAGAATAAT 
GAAGCAGGAG GGGGGACAGA GTCTCATACC CAGGAACCAA GGGAGAAAGG AGAGGATAAA 
GATCTGAATG AGGAGAGAGG AGGAAGCAAT GTGGTACAGC GTATCCTTGA CGTGTTGAAG 
TTTCTCTGGG TTCTTTTGCT TGCCATGGTG GATGGACTGA TCAATTGGCT AGGAACCCTT 
ACAAAGCAGC ATGTTGATAT GTCCACAGTA CTGTGGGTAG AGAGATATAT CATAACCCAA 
AAACTGTCTA AGGGAGAAGA TATCAACCGC AGGGATTTGG AAAAGGTATA TGAGACAGGG 
CAGGATGTTA GGGAGCCTAG GATTTCATCT CCTCCAACTG ATATGTCAAC TAGCATTGCT 
TCAAGTGGCT GTGAGGAAGA GGAGCTTATC TGCAGCACAG CAGAGTGTAC GGAAAATATA 
ACACAACTCA GCACCGGGTA CAACACACGA AGTAACAGCT TGGACTCTAT TAGGGGGTTT 
CCGGAAATCA AGCCAAGTCA TCGTTCTCGT ACTGCTAGTG AATTGCTGCT TTCCAGGCCA 
CTTGATTTCC CAGAGCTCTC CGAGTCTCAG CACTTCTTTC AGTCTCAGAG TCGTATTCTG 
AGGCTCCTGT TGGCCCTCTA TAACTTTGTG GCTGCTCACT CTGAGCTGCT TTGTTACTTC 
ATCATCATTC TCAACAACAT GGTGAGCGCT TCCCTGATAT CACTGTTCCT TCCAATTCTC 
ATCTTCCTGT GGGGTATGCT GTCCATCCAC AAACCCAGTA AGCGCTTCTG GATGACTGCT 
ATCATTTTTA CTGAGGTTAT GGTAGTAATA AAATACCTCT TCCAGTTTGG TTTCTTCCCA 
TGGAATAATT ATACCATTCT GGTACGCAAC GAAGGGAAAC CATTTTTTCC TCCACGTGTC 
CTGGGACTTG AGAAGAGTGA CTCGTACATC AAATATGATC TGATCCAGCT CTTGATGCTC 
TTTTTCCATC GCTCACTGCT CATGAGTTAT GGTCTTTGGG ATCGTAAAGA CTTAGACCTT 
GAAGAGAGAA CACCCGGGAA GGATAAAGGG AGCAAAACAG AGATTCAGGC ATCCCAGGAT 
CTGGAGCCAG CAGGAGGCAG TAGCTCTTCC CTGCGTCAGC GCAAAAGAAA AGAATCCCAG 
GGCAGTGTGC AAGAAAGAGA TTCTTTAAAA GAGAAATCAC TAGATGTGCA GAAGAAGAAA 
AAAGGAAGAG TGGCTCTTAG GGTGAAGATC ATGGGAATGA AAATGCAACA GTTCTTTGCC 
TGCATAGTGC GGAATATATA TCAGCCAGTG AAAGCTTTTT TCCATGATAT TCTACATACC 
AAGAACCGTG CTGCAACAGA TGTTTATGCC TTCATGTTCC TCGCAGATGT GGTTGACTTT 
GTGATCATCG TATTTGGGTT TTGGGCTTTT GGAAAACACT CTGCTGCTGC CGACATAGCA 
TCATCTCTCT CTGATGACCA AGTACCAGAA GCTTTCCTAG TCATGGTCCT CATACAGTTT 
TCCACCATGG TGATAGATCG TGCTCTATAC CTGCGGAAGA CTGTGCTTGG AAAGCTCATC 
TTTCAAGTTA TTCTGGTGAT TGGGATACAT GTCTGGATGT TCTTTATCCT ACCAGCAGTC 
ACAGAAAGGA TGTTCAATCA GAACACTGTG GCCCAGCTCT GGTATTTTGT TAAGTGCATT 
TACTTTGCCC TGTCTGCCTA CCAGATCCGC TGTGGGTATC CTACCCGCAT CCTGGGCAAC 
TTCCTTACCA AGAAATACAA CCACCTCAAC CTATTCCTAT TTCAGGGGTT TCGCTTGGTT 
CCGTTTCTAG TAGAGCTCAG GGCTGTAATG GACTGGGTTT GGACTGATAC CACCTTGTCC 
TTGTCCAACT GGATGTGCGT GGAAGACATT TATGCTAATA TCTTCATTAT CAAATGCAGC 
CGAGAGACTG AAAAGAAATA CCCTCAGCCT AAAGGACAGA AAAAAAAGAA GATAGTGAAA 
TATGGAATGG GTGGTTTGAT CATAGTCTTC CTTATCTGTA TAATCTGGTT TCCCCTTCTC 
TTCATGTCGT TGGTGCGCTC AGTGGTGGGA GTCGTCAACC ACCCTATCGA TGTCACGGTT 
ACTTTTAAAC TCGGGGGTTA TGAGCCTTTA TTCACGATGA GTGCACAGCA ACAGTCCATC 
CAGCCATTCA CCCCACAGCA ATATGAAGCG CTTACCTATG AGTTTGAGAG ACAGCCGACT 
GCCATGCAGT TCATTACTTT GTATAGTTAT GAGGACATAG TGACTGCGCG AATTGAGGGA 
AGTTCAGGAT CTGTATGGGG AATCAGCCCT CCAAGCCGTG AGCAGATGAG AAAGGAGCTG 
CAAAATGGAT CCTCTGACAT TACACTACGC TTCACCTGGG ATTTTCAGAG GGATCTGGCA 
AAAGGTGGCA CTGTGGAGCA CACATCTGAG AAACACACTA AGGACTTGGA GCCTGGGAGC 
CAGGAGAGGA TGGAGCTGGC AGATCTTCTT CAAGGAGTAC GAGACACCCC TGTCTCTATC 
CAGCACTTGT TTCCAAAGTA CATCCGCGCT CCTAATGGCC CAGAAGCAAA TCCAGTGAAA 
CAGCTCCAGC CAGATGAAGA AGAAAGTTAC CTGGATGTGG AGGTGCAGCT AAATCGGATG 
CGAGTTGCAG AGGGAAATAG CACCAGCAGT TTTGTGGAGT GGTGGGTTAT TACTCTAAAA 
GACTGCGAGG GAGAAGACTG CAACATACTA CCTATGGTGA TTTTTAACGA TAAAGTCAGC 
CCTCCTAGCT TAGGATTCCT GGCTGGATAT GGGATAATGG GTTTGTATGT ATCTATTGTC 
CTGGTCATTG GGAAGTTTGT GCGCGGCTTC TTCAGTGAAA TCTCTCACTC CATCATGTTT 
GAGGAGCTGC CTTGTGTGGA TCGGATTCTC AAGCTGTGCC AGGATATCTA TGTGGTGCGG 
GAGCGTGGGG AATTGGAACT GGAAGAGGAA CTCTATGCAA AACTCATCTT CCTGTATCGC 
TCACCAGAAA CCATGATCAA GTGGACTGTA GATAAGGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

piezo1 ( XM_012962282.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817736.1 Xenopus tropicalis piezo-type mechanosensitive ion channel component 1 (piezo1), transcript variant X3, mRNA.

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CloneID	OXa34347
Clone ID Related Accession (Same CDS sequence)	XM_012962282.2
Accession Version	XM_012962282.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	piezo-type mechanosensitive ion channel component 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962282.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACTGC AAGTCCTCTG TGGGGTCCTG TTTTGGCTCC TTCTGCCCGG CTCCCTCTTG 
GCAGCCTGCT TATTCCGTTA CAACTCCTTG TCACTGGTGT ACCTGTTTTA TCTTCTGCTT 
CTGCCATGGT TTGCTGGACC AAATCCCTGC AGCATGAGAG GTCACACAGG ACGTCTGATG 
AAAGCGTTAC TTGCTACCAG CATCCTCTTC CTCTGTGCTC ATCTCATTTT CCAGATCTGC 
CTGTACACAA TACCTGAACT GGATGACCTG CTAGGGCACA ACTGCAGCAG CTGGGATGTG 
TTGGCTCGTC ACGTAGGTAT AAACAGGCTG GATCTTTCTG ATATCCCCAA CATGGTGCGA 
CAAGTGTCCC CAGATTTTGG CATGTTTGTC GTGTCAGCAT TGTGCCTTGG CTTGTGTAAT 
CGCCTAGTGA CCAAGAAGAG ACGACAGAGG TCTTCAGGCT ATTTTGAGCA GGAAGAAGAG 
AGGACTGAAC TTAACCTAAA ATATGAAAGT AGAGAGTGTG ATACAGAGTT TGAGGTGGGG 
GGAGACTTAC ATTCTGTTCC ACGAGACCGC CTTCTTGGGT CCTGGCTTAG AAAGGCAGCA 
TACAGGCTTC TGGAAACAGT GGAGAAATCA CTTGCTGTCT TACTCTTGGC TCTTGCAGGT 
ATCACTGATC CCTCTGCTTT CTCAGCCCCA TATTATGTGG TTTTCATTGG TCTCTGCACA 
TGGTGGGCCT GCCATTTTCC CATTAGCCAA CTAGGCTTTA ATACTATCTG TGTCATTGTT 
GGGTTCTTCA CTGGAGGTCA CATGATTTGC CTTTATATTT ACCAGACACC TTTTATCCAA 
CAAATGTTTC ATCCTGAACA GTTATGGGCA AGGTTGTTTG GTCTGCATAA CATCATCAGT 
CACTCCAACA TTACAGACTG TACCCATGAC TCTCTGAATC AAAACCTTGA CTGGCCTGTG 
TATGTTCATC CCGGAATCTT ATTACTGCTG TACTACTCCC TATTATCAAT ACTGAAACTG 
AAGAGCTCTG ACAAGAAAAG GATTTCAGGG TGTGACCTTC GGAAATCTCA GCAGGAGGAT 
ATGGAGTTGA CCGATATTGA GCCAGTTTCA AAGGAGCAAT ATAGTGCGGG AGAAGAGACA 
AAACCAATGC TTCAGAGGAT GAACACTGAA GAAGAGGCTA AACCCCAAGA CTGCACTGTT 
CATGTGATTA GAAGAAATTC TAATCAACTG CCAGAAGAAC TTGAAACCAA GGAGCCATCA 
CAATTTCACC TGATGATTCA TGTAATACTG AGCCAAAGCT ATATCTGTGC GCTTATTGCA 
ATGATGGTGT GGAGCATTAC ATATCACAGC TGGCTGACTT TTGTGCTGCT GCTCTGGGCA 
TGTTTAATCT GGATGGTGAG GAACAGGCGC CAGTTTGCAA TGCTGTGCTC TCCCTTTATT 
TTGATGTATG GAGTGACTCT CTGCAGCCTG CAGTATGTCT GGAGTATGGA CTTGAGAGAA 
GAGCTTCCGA CACAACTTGG TTTTATGAAG CTGTCACAGC TGGGACTGGT TCGCGCACCA 
TACCCCTGCC TTAACATGGG AGCACTGCTA CTTTACACGC TGACATTTTG GCTTCTGCTG 
CACCAATTTG TAACAGAAAA ATTTCTAAAA CGTAAGAAGT TTATGTCCCC ACTCCTGGAG 
GTCACAGTGG CAGATGCTGG CAAAGAAAAG AACCTGCTAA AACGTTTTGG GGAGCAAGTA 
AAAAGCTTCT ATGCAAAATA CTGGATCTAT GTGTGTGGAG GCATGTTTAT TGTGGTCAGC 
TTTGCTGGTA GGCTGGTAGT TTACAAAATA GTCTACATGC TTCTGTTCCT ACTATGCTTG 
GTGTTATTTC AGGTGTATTA CTCTTTGTGG AGGAAGCTGC TGACTTTTTT CTGGTGGTTA 
GTTGTAGCAT ACACAATGTT GGTTCTTATC ACCATCTACA CCTTTCAATT TGAAGAGTTT 
CCTGAGTACT GGAAAAACTT GACCAACCTT ACAGATGAGC AGTTGGGTGA TATTGGGCTT 
GAGAGGTTTA GTGTGTCGGA ACTTTTTACC AGCATCCTTA TCCCAGGCTT CTTCCTCCTT 
GCCTGCATCC TCCAGTTACA CTACTTTCAT CGCCCTTTTA TGCACATGAC CAACTTGGCA 
CATGCCTCAT CCCAAGGAAG TTTCCATAGC CACGAGAGTG AGCAGCCAAA GGAAGAAGAA 
GAGAAAGCTG GTGCATGCAG TGATGCTGCT GCTGATGAGG AGGAGGATGA GGAAGAATCT 
GCACATGGAG CTGATCTAAG TAAATGGGAC CTAGTTACAC AGCGCCTTTC TGTCCTGTCC 
TTTAAGTTCT CTGAGCTGCT GCACCGATTA CAGGTTTTCC TTTGGAGGCT TTTGGAGCTC 
CATGTGTTGA AACTGGTGGC TTTGTATAGC GTGTGGGTTG CCCTGCAGGA GGTATCTGTG 
ATGAATTTCC TTTTTGTGGT CCTATGGGCT TTTGCTATGC CTTTCTCCCG ATTCCAGCAC 
ATGGCTTCCT GTCTTTGCAC CATCTGGACC TCTATAATTA TTGTTTGCAA AATGCTTTAC 
CAGCTTACTG TTGTCAACCC TCTGGAGTAC TCTAGCAACT GTACTCAGCC TCTTCTAAAT 
AACACCAGTC TCCTAGAAGA CGAGATTTTG AACTCCACCC TTTATAGAGA ACCTATAGAT 
CCAGCAAACT GGTTTGGTAT CAGAAAAGGT TATCCAAACC TAGGTTACAT ACAGAATCAC 
CTGCAAATAC TCCTACTTTT GGTATTTGAG GCAATTGTTT ATCGGCATCA GGAGTTTTAC 
CGTAAACAAT ACCAGCTTGA GAAGCCACAC ATTCAGGCTG TATTTCCAGA TGTCTACAGA 
GAGCACTTGG ACCAAGGCTT GTTCAGCTGT ATCAAATATT TTATCAACTA TTCCTTTTAT 
AAGTTTGGTC TTGAGATCTG CTTCCTTCTT ACTGTGAATG TGATTGGTCA GAGGATGAAC 
TTTATGGTAA TCTTTCATGG CTGCTGGCTG ATAGTGATTC TTACTCGACG ACAGCGAGTC 
AACATTGCAA AATTATGGCC TAAATACTGT GTGTTCCTAA TCTTTTTCCT GTTGTGGCAG 
TACATGATAT GTCTGGGCAT TCCTCCGGTG CTTTGTATAG ATTATCCCTG GAGGTGGGAC 
ACATTTGTTC CTGTCAACTC TGCACTGATA AAGTGGTTAT ATCTTCCAGA TTTCTATACT 
ACACCTAAAT CTGTCAACCT TATCAGTGAT TTTGTTTTGC TGATTGGTGT GGCCCAGCAG 
TGGAAGGTGT TTATTAATGA GAAGAAGGAA GAATGGTACA CTATGGCAGG GGAAAATGTG 
GAAGAGTATG AAGCTCCAAA AGACAGGCCC AACCCTGTAC CTAACTTCAT CAATTGTCAG 
TCTTATCTGG ATATGGTGAA AGTAGCAGTG TTTCGGTACC TATTCTGGTT TGTCTTGACT 
GTGGTCTTTA TAACAGGGGC CACTCGCATC AGTGTTTTTG GCCTAGGATA TTTGATGTCC 
TGTTTCTACC TTCTTCTGTC TGGCACAACC CTTCTGCAAA AGCCAATGAA AGCCAAGCTG 
GTGCTTTGGG ACTGTTTGAT CCTCTACAAC ATCACTGTTA TACTGTCCAA GAACATGCTG 
TCGCTCCTGT CTTGTGTATT TGTTCAACAA ATGCAAACCA ACTTTTGCTG GGTGATCCAG 
CTCTTCAGCC TAGTTTGCAC AGTTAAGGGT TACTACAATC CCCAAGATAT GGATAAAGAC 
CGGGATTGCT CTCTGCCAGT GGAAGAGGCC GGAATCATTT GGGATAGCAT CTGCTTCTTA 
TTTCTCCTTC TCCAACGAAG AGTTTTTTTG AGTTGCTACT TTCGGCATGT TGTTCTTGAC 
CTTCGTGCCT CCTCCCTTCA AACATCAAGA GGTTTTGAGC TCTTCATGGC CAGCATCAGA 
AAGAATGTGT TTTTCCACCA AGAATCTGAG AGACGATCTC TTAACCAGCT CAGGAAGCAA 
ATGGAGAGAA TCCGAGCAAA GCAAGAACGC TATCATGAAG GCAGAAGACA AACTGATAGC 
AGTCAAGTAA AGGAAGTGCT GCATTCAGGA GATTATTTTC TGTTCGAATC AGACAGTGAA 
GAGGATGAAG AGTCTTTATT GGAGCAGGAT CAGAGGCCAC CCAAGCAGAG TGCTTTTCAG 
CTTGCATACC AGGCATGGGT TACAAGTGCC AAATCTGCGC TAAAGGAAAG AAAGAGTCGG 
CAGAAAGAGA GATCTGGAAA GGTAGAAATG GTCACGGTAG AGAATAATGA AGCAGGAGGG 
GGGACAGAGT CTCATACCCA GGAACCAAGG GAGAAAGGAG AGGATAAAGA TCTGAATGAG 
GAGAGAGGAG GAAGCAATGT GGTACAGCGT ATCCTTGACG TGTTGAAGTT TCTCTGGGTT 
CTTTTGCTTG CCATGGTGGA TGGACTGATC AATTGGCTAG GAACCCTTAC AAAGCAGCAT 
GTTGATATGT CCACAGTACT GTGGGTAGAG AGATATATCA TAACCCAAAA ACTGTCTAAG 
GGAGAAGATA TCAACCGCAG GGATTTGGAA AAGGTATATG AGACAGGGCA GGATGTTAGG 
GAGCCTAGGA TTTCATCTCC TCCAACTGAT ATGTCAACTA GCATTGCTTC AAGTGGCTGT 
GAGGAAGAGG AGCTTATCTG CAGCACAGCA GAGTGTACGG AAAATATAAC ACAACTCAGC 
ACCGGGTACA ACACACGAAG TAACAGCTTG GACTCTATTA GGGGGTTTCC GGAAATCAAG 
CCAAGTCATC GTTCTCGTAC TGCTAGTGAA TTGCTGCTTT CCAGGCCACT TGATTTCCCA 
GAGCTCTCCG AGTCTCAGCA CTTCTTTCAG TCTCAGAGTC GTATTCTGAG GCTCCTGTTG 
GCCCTCTATA ACTTTGTGGC TGCTCACTCT GAGCTGCTTT GTTACTTCAT CATCATTCTC 
AACAACATGG TGAGCGCTTC CCTGATATCA CTGTTCCTTC CAATTCTCAT CTTCCTGTGG 
GGTATGCTGT CCATCCACAA ACCCAGTAAG CGCTTCTGGA TGACTGCTAT CATTTTTACT 
GAGGTTATGG TAGTAATAAA ATACCTCTTC CAGTTTGGTT TCTTCCCATG GAATAATTAT 
ACCATTCTGG TACGCAACGA AGGGAAACCA TTTTTTCCTC CACGTGTCCT GGGACTTGAG 
AAGAGTGACT CGTACATCAA ATATGATCTG ATCCAGCTCT TGATGCTCTT TTTCCATCGC 
TCACTGCTCA TGAGTTATGG TCTTTGGGAT CGTAAAGACT TAGACCTTGA AGAGAGAACA 
CCCGGGAAGG ATAAAGGGAG CAAAACAGAG ATTCAGGCAT CCCAGGATCT GGAGCCAGCA 
GGAGGCAGTA GCTCTTCCCT GCGTCAGCGC AAAAGAAAAG AATCCCAGGG CAGTGTGCAA 
GAAAGAGATT CTTTAAAAGA GAAATCACTA GATGTGCAGA AGAAGAAAAA AGGAAGAGTG 
GCTCTTAGGG TGAAGATCAT GGGAATGAAA ATGCAACAGT TCTTTGCCTG CATAGTGCGG 
AATATATATC AGCCAGTGAA AGCTTTTTTC CATGATATTC TACATACCAA GAACCGTGCT 
GCAACAGATG TTTATGCCTT CATGTTCCTC GCAGATGTGG TTGACTTTGT GATCATCGTA 
TTTGGGTTTT GGGCTTTTGG AAAACACTCT GCTGCTGCCG ACATAGCATC ATCTCTCTCT 
GATGACCAAG TACCAGAAGC TTTCCTAGTC ATGGTCCTCA TACAGTTTTC CACCATGGTG 
ATAGATCGTG CTCTATACCT GCGGAAGACT GTGCTTGGAA AGCTCATCTT TCAAGTTATT 
CTGGTGATTG GGATACATGT CTGGATGTTC TTTATCCTAC CAGCAGTCAC AGAAAGGATG 
TTCAATCAGA ACACTGTGGC CCAGCTCTGG TATTTTGTTA AGTGCATTTA CTTTGCCCTG 
TCTGCCTACC AGATCCGCTG TGGGTATCCT ACCCGCATCC TGGGCAACTT CCTTACCAAG 
AAATACAACC ACCTCAACCT ATTCCTATTT CAGGGGTTTC GCTTGGTTCC GTTTCTAGTA 
GAGCTCAGGG CTGTAATGGA CTGGGTTTGG ACTGATACCA CCTTGTCCTT GTCCAACTGG 
ATGTGCGTGG AAGACATTTA TGCTAATATC TTCATTATCA AATGCAGCCG AGAGACTGAA 
AAGAAATACC CTCAGCCTAA AGGACAGAAA AAAAAGAAGA TAGTGAAATA TGGAATGGGT 
GGTTTGATCA TAGTCTTCCT TATCTGTATA ATCTGGTTTC CCCTTCTCTT CATGTCGTTG 
GTGCGCTCAG TGGTGGGAGT CGTCAACCAC CCTATCGATG TCACGGTTAC TTTTAAACTC 
GGGGGTTATG AGCCTTTATT CACGATGAGT GCACAGCAAC AGTCCATCCA GCCATTCACC 
CCACAGCAAT ATGAAGCGCT TACCTATGAG TTTGAGAGAC AGCCGACTGC CATGCAGTTC 
ATTACTTTGT ATAGTTATGA GGACATAGTG ACTGCGCGAA TTGAGGGAAG TTCAGGATCT 
GTATGGGGAA TCAGCCCTCC AAGCCGTGAG CAGATGAGAA AGGAGCTGCA AAATGGATCC 
TCTGACATTA CACTACGCTT CACCTGGGAT TTTCAGAGGG ATCTGGCAAA AGGTGGCACT 
GTGGAGCACA CATCTGAGAA ACACACTAAG GACTTGGAGC CTGGGAGCCA GGAGAGGATG 
GAGCTGGCAG ATCTTCTTCA AGGAGTACGA GACACCCCTG TCTCTATCCA GCACTTGTTT 
CCAAAGTACA TCCGCGCTCC TAATGGCCCA GAAGCAAATC CAGTGAAACA GCTCCAGCCA 
GATGAAGAAG AAAGTTACCT GGATGTGGAG GTGCAGCTAA ATCGGATGCG AGTTGCAGAG 
GGAAATAGCA CCAGCAGTTT TGTGGAGTGG TGGGTTATTA CTCTAAAAGA CTGCGAGGGA 
GAAGACTGCA ACATACTACC TATGGTGATT TTTAACGATA AAGTCAGCCC TCCTAGCTTA 
GGATTCCTGG CTGGATATGG GATAATGGGT TTGTATGTAT CTATTGTCCT GGTCATTGGG 
AAGTTTGTGC GCGGCTTCTT CAGTGAAATC TCTCACTCCA TCATGTTTGA GGAGCTGCCT 
TGTGTGGATC GGATTCTCAA GCTGTGCCAG GATATCTATG TGGTGCGGGA GCGTGGGGAA 
TTGGAACTGG AAGAGGAACT CTATGCAAAA CTCATCTTCC TGTATCGCTC ACCAGAAACC 
ATGATCAAGT GGACTGTAGA TAAGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817736.1
CDS	1201..8550
Translation	MELQVLCGVLFWLLLPGSLLAACLFRYNSLSLVYLFYLLLLPWFAGPNPCSMRGHTGRLMKALLATSILFLCAHLIFQICLYTIPELDDLLGHNCSSWDVLARHVGINRLDLSDIPNMVRQVSPDFGMFVVSALCLGLCNRLVTKKRRQRSSGYFEQEEERTELNLKYESRECDTEFEVGGDLHSVPRDRLLGSWLRKAAYRLLETVEKSLAVLLLALAGITDPSAFSAPYYVVFIGLCTWWACHFPISQLGFNTICVIVGFFTGGHMICLYIYQTPFIQQMFHPEQLWARLFGLHNIISHSNITDCTHDSLNQNLDWPVYVHPGILLLLYYSLLSILKLKSSDKKRISGCDLRKSQQEDMELTDIEPVSKEQYSAGEETKPMLQRMNTEEEAKPQDCTVHVIRRNSNQLPEELETKEPSQFHLMIHVILSQSYICALIAMMVWSITYHSWLTFVLLLWACLIWMVRNRRQFAMLCSPFILMYGVTLCSLQYVWSMDLREELPTQLGFMKLSQLGLVRAPYPCLNMGALLLYTLTFWLLLHQFVTEKFLKRKKFMSPLLEVTVADAGKEKNLLKRFGEQVKSFYAKYWIYVCGGMFIVVSFAGRLVVYKIVYMLLFLLCLVLFQVYYSLWRKLLTFFWWLVVAYTMLVLITIYTFQFEEFPEYWKNLTNLTDEQLGDIGLERFSVSELFTSILIPGFFLLACILQLHYFHRPFMHMTNLAHASSQGSFHSHESEQPKEEEEKAGACSDAAADEEEDEEESAHGADLSKWDLVTQRLSVLSFKFSELLHRLQVFLWRLLELHVLKLVALYSVWVALQEVSVMNFLFVVLWAFAMPFSRFQHMASCLCTIWTSIIIVCKMLYQLTVVNPLEYSSNCTQPLLNNTSLLEDEILNSTLYREPIDPANWFGIRKGYPNLGYIQNHLQILLLLVFEAIVYRHQEFYRKQYQLEKPHIQAVFPDVYREHLDQGLFSCIKYFINYSFYKFGLEICFLLTVNVIGQRMNFMVIFHGCWLIVILTRRQRVNIAKLWPKYCVFLIFFLLWQYMICLGIPPVLCIDYPWRWDTFVPVNSALIKWLYLPDFYTTPKSVNLISDFVLLIGVAQQWKVFINEKKEEWYTMAGENVEEYEAPKDRPNPVPNFINCQSYLDMVKVAVFRYLFWFVLTVVFITGATRISVFGLGYLMSCFYLLLSGTTLLQKPMKAKLVLWDCLILYNITVILSKNMLSLLSCVFVQQMQTNFCWVIQLFSLVCTVKGYYNPQDMDKDRDCSLPVEEAGIIWDSICFLFLLLQRRVFLSCYFRHVVLDLRASSLQTSRGFELFMASIRKNVFFHQESERRSLNQLRKQMERIRAKQERYHEGRRQTDSSQVKEVLHSGDYFLFESDSEEDEESLLEQDQRPPKQSAFQLAYQAWVTSAKSALKERKSRQKERSGKVEMVTVENNEAGGGTESHTQEPREKGEDKDLNEERGGSNVVQRILDVLKFLWVLLLAMVDGLINWLGTLTKQHVDMSTVLWVERYIITQKLSKGEDINRRDLEKVYETGQDVREPRISSPPTDMSTSIASSGCEEEELICSTAECTENITQLSTGYNTRSNSLDSIRGFPEIKPSHRSRTASELLLSRPLDFPELSESQHFFQSQSRILRLLLALYNFVAAHSELLCYFIIILNNMVSASLISLFLPILIFLWGMLSIHKPSKRFWMTAIIFTEVMVVIKYLFQFGFFPWNNYTILVRNEGKPFFPPRVLGLEKSDSYIKYDLIQLLMLFFHRSLLMSYGLWDRKDLDLEERTPGKDKGSKTEIQASQDLEPAGGSSSSLRQRKRKESQGSVQERDSLKEKSLDVQKKKKGRVALRVKIMGMKMQQFFACIVRNIYQPVKAFFHDILHTKNRAATDVYAFMFLADVVDFVIIVFGFWAFGKHSAAADIASSLSDDQVPEAFLVMVLIQFSTMVIDRALYLRKTVLGKLIFQVILVIGIHVWMFFILPAVTERMFNQNTVAQLWYFVKCIYFALSAYQIRCGYPTRILGNFLTKKYNHLNLFLFQGFRLVPFLVELRAVMDWVWTDTTLSLSNWMCVEDIYANIFIIKCSRETEKKYPQPKGQKKKKIVKYGMGGLIIVFLICIIWFPLLFMSLVRSVVGVVNHPIDVTVTFKLGGYEPLFTMSAQQQSIQPFTPQQYEALTYEFERQPTAMQFITLYSYEDIVTARIEGSSGSVWGISPPSREQMRKELQNGSSDITLRFTWDFQRDLAKGGTVEHTSEKHTKDLEPGSQERMELADLLQGVRDTPVSIQHLFPKYIRAPNGPEANPVKQLQPDEEESYLDVEVQLNRMRVAEGNSTSSFVEWWVITLKDCEGEDCNILPMVIFNDKVSPPSLGFLAGYGIMGLYVSIVLVIGKFVRGFFSEISHSIMFEELPCVDRILKLCQDIYVVRERGELELEEELYAKLIFLYRSPETMIKWTVDKD

Target ORF information:

RefSeq Version	XM_012962282.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis piezo-type mechanosensitive ion channel component 1 (piezo1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012962282.2

ORF Insert Sequence:

ATGGAACTGC AAGTCCTCTG TGGGGTCCTG TTTTGGCTCC TTCTGCCCGG CTCCCTCTTG 
GCAGCCTGCT TATTCCGTTA CAACTCCTTG TCACTGGTGT ACCTGTTTTA TCTTCTGCTT 
CTGCCATGGT TTGCTGGACC AAATCCCTGC AGCATGAGAG GTCACACAGG ACGTCTGATG 
AAAGCGTTAC TTGCTACCAG CATCCTCTTC CTCTGTGCTC ATCTCATTTT CCAGATCTGC 
CTGTACACAA TACCTGAACT GGATGACCTG CTAGGGCACA ACTGCAGCAG CTGGGATGTG 
TTGGCTCGTC ACGTAGGTAT AAACAGGCTG GATCTTTCTG ATATCCCCAA CATGGTGCGA 
CAAGTGTCCC CAGATTTTGG CATGTTTGTC GTGTCAGCAT TGTGCCTTGG CTTGTGTAAT 
CGCCTAGTGA CCAAGAAGAG ACGACAGAGG TCTTCAGGCT ATTTTGAGCA GGAAGAAGAG 
AGGACTGAAC TTAACCTAAA ATATGAAAGT AGAGAGTGTG ATACAGAGTT TGAGGTGGGG 
GGAGACTTAC ATTCTGTTCC ACGAGACCGC CTTCTTGGGT CCTGGCTTAG AAAGGCAGCA 
TACAGGCTTC TGGAAACAGT GGAGAAATCA CTTGCTGTCT TACTCTTGGC TCTTGCAGGT 
ATCACTGATC CCTCTGCTTT CTCAGCCCCA TATTATGTGG TTTTCATTGG TCTCTGCACA 
TGGTGGGCCT GCCATTTTCC CATTAGCCAA CTAGGCTTTA ATACTATCTG TGTCATTGTT 
GGGTTCTTCA CTGGAGGTCA CATGATTTGC CTTTATATTT ACCAGACACC TTTTATCCAA 
CAAATGTTTC ATCCTGAACA GTTATGGGCA AGGTTGTTTG GTCTGCATAA CATCATCAGT 
CACTCCAACA TTACAGACTG TACCCATGAC TCTCTGAATC AAAACCTTGA CTGGCCTGTG 
TATGTTCATC CCGGAATCTT ATTACTGCTG TACTACTCCC TATTATCAAT ACTGAAACTG 
AAGAGCTCTG ACAAGAAAAG GATTTCAGGG TGTGACCTTC GGAAATCTCA GCAGGAGGAT 
ATGGAGTTGA CCGATATTGA GCCAGTTTCA AAGGAGCAAT ATAGTGCGGG AGAAGAGACA 
AAACCAATGC TTCAGAGGAT GAACACTGAA GAAGAGGCTA AACCCCAAGA CTGCACTGTT 
CATGTGATTA GAAGAAATTC TAATCAACTG CCAGAAGAAC TTGAAACCAA GGAGCCATCA 
CAATTTCACC TGATGATTCA TGTAATACTG AGCCAAAGCT ATATCTGTGC GCTTATTGCA 
ATGATGGTGT GGAGCATTAC ATATCACAGC TGGCTGACTT TTGTGCTGCT GCTCTGGGCA 
TGTTTAATCT GGATGGTGAG GAACAGGCGC CAGTTTGCAA TGCTGTGCTC TCCCTTTATT 
TTGATGTATG GAGTGACTCT CTGCAGCCTG CAGTATGTCT GGAGTATGGA CTTGAGAGAA 
GAGCTTCCGA CACAACTTGG TTTTATGAAG CTGTCACAGC TGGGACTGGT TCGCGCACCA 
TACCCCTGCC TTAACATGGG AGCACTGCTA CTTTACACGC TGACATTTTG GCTTCTGCTG 
CACCAATTTG TAACAGAAAA ATTTCTAAAA CGTAAGAAGT TTATGTCCCC ACTCCTGGAG 
GTCACAGTGG CAGATGCTGG CAAAGAAAAG AACCTGCTAA AACGTTTTGG GGAGCAAGTA 
AAAAGCTTCT ATGCAAAATA CTGGATCTAT GTGTGTGGAG GCATGTTTAT TGTGGTCAGC 
TTTGCTGGTA GGCTGGTAGT TTACAAAATA GTCTACATGC TTCTGTTCCT ACTATGCTTG 
GTGTTATTTC AGGTGTATTA CTCTTTGTGG AGGAAGCTGC TGACTTTTTT CTGGTGGTTA 
GTTGTAGCAT ACACAATGTT GGTTCTTATC ACCATCTACA CCTTTCAATT TGAAGAGTTT 
CCTGAGTACT GGAAAAACTT GACCAACCTT ACAGATGAGC AGTTGGGTGA TATTGGGCTT 
GAGAGGTTTA GTGTGTCGGA ACTTTTTACC AGCATCCTTA TCCCAGGCTT CTTCCTCCTT 
GCCTGCATCC TCCAGTTACA CTACTTTCAT CGCCCTTTTA TGCACATGAC CAACTTGGCA 
CATGCCTCAT CCCAAGGAAG TTTCCATAGC CACGAGAGTG AGCAGCCAAA GGAAGAAGAA 
GAGAAAGCTG GTGCATGCAG TGATGCTGCT GCTGATGAGG AGGAGGATGA GGAAGAATCT 
GCACATGGAG CTGATCTAAG TAAATGGGAC CTAGTTACAC AGCGCCTTTC TGTCCTGTCC 
TTTAAGTTCT CTGAGCTGCT GCACCGATTA CAGGTTTTCC TTTGGAGGCT TTTGGAGCTC 
CATGTGTTGA AACTGGTGGC TTTGTATAGC GTGTGGGTTG CCCTGCAGGA GGTATCTGTG 
ATGAATTTCC TTTTTGTGGT CCTATGGGCT TTTGCTATGC CTTTCTCCCG ATTCCAGCAC 
ATGGCTTCCT GTCTTTGCAC CATCTGGACC TCTATAATTA TTGTTTGCAA AATGCTTTAC 
CAGCTTACTG TTGTCAACCC TCTGGAGTAC TCTAGCAACT GTACTCAGCC TCTTCTAAAT 
AACACCAGTC TCCTAGAAGA CGAGATTTTG AACTCCACCC TTTATAGAGA ACCTATAGAT 
CCAGCAAACT GGTTTGGTAT CAGAAAAGGT TATCCAAACC TAGGTTACAT ACAGAATCAC 
CTGCAAATAC TCCTACTTTT GGTATTTGAG GCAATTGTTT ATCGGCATCA GGAGTTTTAC 
CGTAAACAAT ACCAGCTTGA GAAGCCACAC ATTCAGGCTG TATTTCCAGA TGTCTACAGA 
GAGCACTTGG ACCAAGGCTT GTTCAGCTGT ATCAAATATT TTATCAACTA TTCCTTTTAT 
AAGTTTGGTC TTGAGATCTG CTTCCTTCTT ACTGTGAATG TGATTGGTCA GAGGATGAAC 
TTTATGGTAA TCTTTCATGG CTGCTGGCTG ATAGTGATTC TTACTCGACG ACAGCGAGTC 
AACATTGCAA AATTATGGCC TAAATACTGT GTGTTCCTAA TCTTTTTCCT GTTGTGGCAG 
TACATGATAT GTCTGGGCAT TCCTCCGGTG CTTTGTATAG ATTATCCCTG GAGGTGGGAC 
ACATTTGTTC CTGTCAACTC TGCACTGATA AAGTGGTTAT ATCTTCCAGA TTTCTATACT 
ACACCTAAAT CTGTCAACCT TATCAGTGAT TTTGTTTTGC TGATTGGTGT GGCCCAGCAG 
TGGAAGGTGT TTATTAATGA GAAGAAGGAA GAATGGTACA CTATGGCAGG GGAAAATGTG 
GAAGAGTATG AAGCTCCAAA AGACAGGCCC AACCCTGTAC CTAACTTCAT CAATTGTCAG 
TCTTATCTGG ATATGGTGAA AGTAGCAGTG TTTCGGTACC TATTCTGGTT TGTCTTGACT 
GTGGTCTTTA TAACAGGGGC CACTCGCATC AGTGTTTTTG GCCTAGGATA TTTGATGTCC 
TGTTTCTACC TTCTTCTGTC TGGCACAACC CTTCTGCAAA AGCCAATGAA AGCCAAGCTG 
GTGCTTTGGG ACTGTTTGAT CCTCTACAAC ATCACTGTTA TACTGTCCAA GAACATGCTG 
TCGCTCCTGT CTTGTGTATT TGTTCAACAA ATGCAAACCA ACTTTTGCTG GGTGATCCAG 
CTCTTCAGCC TAGTTTGCAC AGTTAAGGGT TACTACAATC CCCAAGATAT GGATAAAGAC 
CGGGATTGCT CTCTGCCAGT GGAAGAGGCC GGAATCATTT GGGATAGCAT CTGCTTCTTA 
TTTCTCCTTC TCCAACGAAG AGTTTTTTTG AGTTGCTACT TTCGGCATGT TGTTCTTGAC 
CTTCGTGCCT CCTCCCTTCA AACATCAAGA GGTTTTGAGC TCTTCATGGC CAGCATCAGA 
AAGAATGTGT TTTTCCACCA AGAATCTGAG AGACGATCTC TTAACCAGCT CAGGAAGCAA 
ATGGAGAGAA TCCGAGCAAA GCAAGAACGC TATCATGAAG GCAGAAGACA AACTGATAGC 
AGTCAAGTAA AGGAAGTGCT GCATTCAGGA GATTATTTTC TGTTCGAATC AGACAGTGAA 
GAGGATGAAG AGTCTTTATT GGAGCAGGAT CAGAGGCCAC CCAAGCAGAG TGCTTTTCAG 
CTTGCATACC AGGCATGGGT TACAAGTGCC AAATCTGCGC TAAAGGAAAG AAAGAGTCGG 
CAGAAAGAGA GATCTGGAAA GGTAGAAATG GTCACGGTAG AGAATAATGA AGCAGGAGGG 
GGGACAGAGT CTCATACCCA GGAACCAAGG GAGAAAGGAG AGGATAAAGA TCTGAATGAG 
GAGAGAGGAG GAAGCAATGT GGTACAGCGT ATCCTTGACG TGTTGAAGTT TCTCTGGGTT 
CTTTTGCTTG CCATGGTGGA TGGACTGATC AATTGGCTAG GAACCCTTAC AAAGCAGCAT 
GTTGATATGT CCACAGTACT GTGGGTAGAG AGATATATCA TAACCCAAAA ACTGTCTAAG 
GGAGAAGATA TCAACCGCAG GGATTTGGAA AAGGTATATG AGACAGGGCA GGATGTTAGG 
GAGCCTAGGA TTTCATCTCC TCCAACTGAT ATGTCAACTA GCATTGCTTC AAGTGGCTGT 
GAGGAAGAGG AGCTTATCTG CAGCACAGCA GAGTGTACGG AAAATATAAC ACAACTCAGC 
ACCGGGTACA ACACACGAAG TAACAGCTTG GACTCTATTA GGGGGTTTCC GGAAATCAAG 
CCAAGTCATC GTTCTCGTAC TGCTAGTGAA TTGCTGCTTT CCAGGCCACT TGATTTCCCA 
GAGCTCTCCG AGTCTCAGCA CTTCTTTCAG TCTCAGAGTC GTATTCTGAG GCTCCTGTTG 
GCCCTCTATA ACTTTGTGGC TGCTCACTCT GAGCTGCTTT GTTACTTCAT CATCATTCTC 
AACAACATGG TGAGCGCTTC CCTGATATCA CTGTTCCTTC CAATTCTCAT CTTCCTGTGG 
GGTATGCTGT CCATCCACAA ACCCAGTAAG CGCTTCTGGA TGACTGCTAT CATTTTTACT 
GAGGTTATGG TAGTAATAAA ATACCTCTTC CAGTTTGGTT TCTTCCCATG GAATAATTAT 
ACCATTCTGG TACGCAACGA AGGGAAACCA TTTTTTCCTC CACGTGTCCT GGGACTTGAG 
AAGAGTGACT CGTACATCAA ATATGATCTG ATCCAGCTCT TGATGCTCTT TTTCCATCGC 
TCACTGCTCA TGAGTTATGG TCTTTGGGAT CGTAAAGACT TAGACCTTGA AGAGAGAACA 
CCCGGGAAGG ATAAAGGGAG CAAAACAGAG ATTCAGGCAT CCCAGGATCT GGAGCCAGCA 
GGAGGCAGTA GCTCTTCCCT GCGTCAGCGC AAAAGAAAAG AATCCCAGGG CAGTGTGCAA 
GAAAGAGATT CTTTAAAAGA GAAATCACTA GATGTGCAGA AGAAGAAAAA AGGAAGAGTG 
GCTCTTAGGG TGAAGATCAT GGGAATGAAA ATGCAACAGT TCTTTGCCTG CATAGTGCGG 
AATATATATC AGCCAGTGAA AGCTTTTTTC CATGATATTC TACATACCAA GAACCGTGCT 
GCAACAGATG TTTATGCCTT CATGTTCCTC GCAGATGTGG TTGACTTTGT GATCATCGTA 
TTTGGGTTTT GGGCTTTTGG AAAACACTCT GCTGCTGCCG ACATAGCATC ATCTCTCTCT 
GATGACCAAG TACCAGAAGC TTTCCTAGTC ATGGTCCTCA TACAGTTTTC CACCATGGTG 
ATAGATCGTG CTCTATACCT GCGGAAGACT GTGCTTGGAA AGCTCATCTT TCAAGTTATT 
CTGGTGATTG GGATACATGT CTGGATGTTC TTTATCCTAC CAGCAGTCAC AGAAAGGATG 
TTCAATCAGA ACACTGTGGC CCAGCTCTGG TATTTTGTTA AGTGCATTTA CTTTGCCCTG 
TCTGCCTACC AGATCCGCTG TGGGTATCCT ACCCGCATCC TGGGCAACTT CCTTACCAAG 
AAATACAACC ACCTCAACCT ATTCCTATTT CAGGGGTTTC GCTTGGTTCC GTTTCTAGTA 
GAGCTCAGGG CTGTAATGGA CTGGGTTTGG ACTGATACCA CCTTGTCCTT GTCCAACTGG 
ATGTGCGTGG AAGACATTTA TGCTAATATC TTCATTATCA AATGCAGCCG AGAGACTGAA 
AAGAAATACC CTCAGCCTAA AGGACAGAAA AAAAAGAAGA TAGTGAAATA TGGAATGGGT 
GGTTTGATCA TAGTCTTCCT TATCTGTATA ATCTGGTTTC CCCTTCTCTT CATGTCGTTG 
GTGCGCTCAG TGGTGGGAGT CGTCAACCAC CCTATCGATG TCACGGTTAC TTTTAAACTC 
GGGGGTTATG AGCCTTTATT CACGATGAGT GCACAGCAAC AGTCCATCCA GCCATTCACC 
CCACAGCAAT ATGAAGCGCT TACCTATGAG TTTGAGAGAC AGCCGACTGC CATGCAGTTC 
ATTACTTTGT ATAGTTATGA GGACATAGTG ACTGCGCGAA TTGAGGGAAG TTCAGGATCT 
GTATGGGGAA TCAGCCCTCC AAGCCGTGAG CAGATGAGAA AGGAGCTGCA AAATGGATCC 
TCTGACATTA CACTACGCTT CACCTGGGAT TTTCAGAGGG ATCTGGCAAA AGGTGGCACT 
GTGGAGCACA CATCTGAGAA ACACACTAAG GACTTGGAGC CTGGGAGCCA GGAGAGGATG 
GAGCTGGCAG ATCTTCTTCA AGGAGTACGA GACACCCCTG TCTCTATCCA GCACTTGTTT 
CCAAAGTACA TCCGCGCTCC TAATGGCCCA GAAGCAAATC CAGTGAAACA GCTCCAGCCA 
GATGAAGAAG AAAGTTACCT GGATGTGGAG GTGCAGCTAA ATCGGATGCG AGTTGCAGAG 
GGAAATAGCA CCAGCAGTTT TGTGGAGTGG TGGGTTATTA CTCTAAAAGA CTGCGAGGGA 
GAAGACTGCA ACATACTACC TATGGTGATT TTTAACGATA AAGTCAGCCC TCCTAGCTTA 
GGATTCCTGG CTGGATATGG GATAATGGGT TTGTATGTAT CTATTGTCCT GGTCATTGGG 
AAGTTTGTGC GCGGCTTCTT CAGTGAAATC TCTCACTCCA TCATGTTTGA GGAGCTGCCT 
TGTGTGGATC GGATTCTCAA GCTGTGCCAG GATATCTATG TGGTGCGGGA GCGTGGGGAA 
TTGGAACTGG AAGAGGAACT CTATGCAAAA CTCATCTTCC TGTATCGCTC ACCAGAAACC 
ATGATCAAGT GGACTGTAGA TAAGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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03-6811-6573
050-3174-7589(受注専用)

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オンライン注文

piezo1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

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