piwil1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	piwil1
Entrez Gene ID	100488735
Full Name	piwi-like RNA-mediated gene silencing 1
Synonyms	Xiwi1a,hiwi,miwi,piwi,xiwi1,ziwi
General protein information	Preferred Names piwi-like RNA-mediated gene silencing 1 Names piwi-like protein 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018090379.2	XP_017945868.1	piwi-like protein 1 isoform X2
XM_018090379.1	XP_017945868.1	piwi-like protein 1 isoform X2
XM_002940181.4	XP_002940227.2	piwi-like protein 1 isoform X1
XM_002940181.3	XP_002940227.2	piwi-like protein 1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PIWIL1	NP_004755.2
Pan troglodytes (chimpanzee)	PIWIL1	XP_001137913.1
Xenopus tropicalis (tropical clawed frog)	LOC100496286	XP_002942570.2
Canis lupus familiaris (dog)	PIWIL1	XP_534638.2
Mus musculus (house mouse)	Piwil1	NP_067286.1
Rattus norvegicus (Norway rat)	Piwil1	NP_001102323.1
Danio rerio (zebrafish)	piwil1	NP_899181.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011204	XP_564296.2
Caenorhabditis elegans (roundworm)	prg-1	NP_492121.1
Caenorhabditis elegans (roundworm)	prg-2	NP_500994.1
Bos taurus (cattle)	PIWIL1	XP_002694505.2
Gallus gallus (chicken)	PIWIL1	NP_001092322.1
Drosophila melanogaster (fruit fly)	aub	NP_476734.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009509	XP_310187.3
Xenopus tropicalis (tropical clawed frog)	piwil1	XP_002940227.2

Gene Ontology

The following piwil1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the piwil1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa03573	XM_002940181.4 Latest version!	Xenopus tropicalis piwi-like RNA-mediated gene silencing 1 (piwil1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa34304	XM_018090379.2 Latest version!	Xenopus tropicalis piwi-like RNA-mediated gene silencing 1 (piwil1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OXa34304	XM_018090379.1	Xenopus tropicalis piwi-like RNA-mediated gene silencing 1 (piwil1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa03573	XM_002940181.3	Xenopus tropicalis piwi-like RNA-mediated gene silencing 1 (piwil1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

piwil1 ( XM_002940181.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002940227.2 Xenopus tropicalis piwi-like RNA-mediated gene silencing 1 (piwil1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa03573
Clone ID Related Accession (Same CDS sequence)	XM_002940181.4 , XM_002940181.3
Accession Version	XM_002940181.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	piwi-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2015 this sequence version replaced XM_002940181.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGGAA GAGCTAGAGC AAGAGCTCGA GGCAGAGCCC GTGGTCAGGA ACCACCAGCA 
CCTGGAAAGG AGCCTCAACC TGGATATACA CAAAAGACAT CAACAGAAGA GCAAGCAGAA 
CCAGAGATTG TTGGGCGTGG ACGTCAGAAG AGAGCCTCTG TTGCTCACTC AACTCCAGTG 
ACACAGGCAG GAGACCTGCA GATATCAGCA GGATTTCAAG AAATATCTCT TGGGGATCGT 
GGTGGACGTA GACGTGATTT TCATGACCTT GGTATAAATA CTCGTCAAGC AATAGAACAC 
GTCAAAGAGT CAAAAACAGG TTCTTCTGGA AGCATAATTC AGCTAAGTAC AAACCATATA 
AAACTTATCT CTAGACCTCA GTGGGTATTA TTCCAATATC ATATTGACTA TGCCCCACAG 
ATGGAATCTA GGGGATTGCG CTGTGCTTTA CTCTTTCAGC ATGAGGAACT TATTGGAAAA 
GCACGTGCTT TTGATGGAAC AATGTTATTT TTACCTAAAA GACTTGATAA GGTTAATGAG 
GTTTTTAGTC AAACCCGCAA TGGTGAGACA GTGAAGATCA CTATTACTTT AACAAACGAG 
TTACCACCTA CTTCACCAAC GTGCTTCCAG TTCTACAACA TCATTTTTCG AAGACTTCTG 
AAGATGATGA ATATGAAGCA GATTGGACGT AATTACTACA ATCCTAATGA TCAGATAGAA 
ATTAGTTCAC ATGGCCTTAC TATATATCCA GGGTTTTCCA CTTCAATTCT GCAATATGAA 
AACAACATAA TGTTAAGCAT AGATGTGAGC CACAAAGTTT TAAGAAGCGA AACAGTTCTG 
GATTACATGT ACAATGTGAA CCAAAAAGTG GAGTCACATA AATTCCATGA TATCTGCAGC 
AAAGACCTGA TTGGACAAAT TGTTCTTACA AAGTACAACA ATAAGACTTA CCGGATTGAT 
GATATCAACT GGGATTTTAC TCCAGAGTCT ACATTTAAGA AATCAGATGG TTCAGAAATA 
AGCTTTGTTG ATTACTATAG AACACAATAT AATAAAGGAA TCACAGACTT AAACCAGCCT 
GCTCTTGTTC ATAATCCAAA AAAACCCAGG GGTCCACAGA ATGTTCCAGC AGGACCAATT 
CTCTTGATAC CAGAATTTTG TTTCCTAACA GGTTTAACTG ATCGAATGAG GAGTGATTTT 
AATGTAATGA AGGACCTAGC AATTCATACC AGACTGGCAC CAGAGCAAAG AGAAATACAA 
GTTGGAAAAT TTCTAAAGAA TATTCACAAG GATGATAGTG TGCAGAAAGA GCTTCAGGAT 
TGGGGCTTAA ACTTTGACTC AAAACTTTTG CCTTTTGCTG GCAGAGTAGC TCCAGCAGAG 
AAGATTTTGC AGGCTGGAAA AACAGCTGAC TACAATCCTC AGTTTGCTGA CTGGTCAAGA 
GAATTACGAG GACCGACACT AATCCGTGTG AAACATTTGG ATAATTGGGT ATTGTTATAC 
ACACGCAGAA ACTATGATGC TGCCAATACT TTGACACAAA ATCTGTTCAA AGTGTCAGGT 
CAAATGGGAA TCAAGATGAA TAGAGCAGTA ATGGTGGAAG TAGATGACAC TACAGATGCA 
TATGTAAAAG TTTTGCAGCA AAAGGTAACA CCTGATGTAC AGATGGTAGT TTGTCTTCTC 
TCTAGTAATC GGAAGGATAA ATATGATGCT ATAAAAAAAT ACCTGTGCAT TGACTGCCCA 
GTTCCAAGTC AATGCGTGTT AGCAAAGACT TTAAATAAAC CACAGACCGT TGTTTCAGTT 
GCAACAAAAA TTGCATTACA GATGAACTGC AAGATGGGGG GAGAGTTATG GACTGTTGAA 
ATACCTCTGA AGGAATTGAT GATTGTTGGC ATTGATTGTT ATCACGACAC ATTATCAGGA 
AAAAGATCTA TTGGGGCATT TGTTGCCAGT TTAAACCCAA GCATGACACG GTGGTTTTCT 
CGTTGTGTTC TCCAGGCACA GAAACAAGAG ATTGTTGATG GTCTCAAAGT CTGCATGCAT 
GCTGCCCTAA AAGCCTGGTT TAATTGCAAC AAAAGTCTGC CTTCCCGTAT AATAATCTAC 
CGAGATGGAG TAGGAGATGG TCAGTTGAAA ACAATGGTCG AATACGAAAT ACCACAGCTC 
CAGGATTGTA TAAGGTCTGC TGAAAAAGAT TACAGCCCAA AACTAACTGT TATTGTCGTA 
AAAAAACGAG TCAGTGCTAG ATTCTTTGCA CATGTTGGTG GTAGACTTCA GAACCCTCCT 
CCAGGCACCA TTGTTGATGT GGAAATCACG AGACCAGAAT GGTATGACTT CTTCATAATC 
AGCCAGTCTG TAAGAGTAGG TTCTGTGTCG CCAACTCACT ACAACGTTGT GTATGACAGC 
GGCGCATTGA AACCAGATCA TATGCAAAGA CTGACTTACA AGCTTTGCCA TCTGTACTAC 
AACTGGCCAG GTGTAATCAG AGTGCCAGCT CCTTGTCAGT ATGCCCATAA ATTAGCATTT 
TTGGTTGGTC AGAGCATCCA CAGAGAACCA CATCTTACAC TTTCGGATCG CCTATATTAT 
CTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002940227.2
CDS	223..2808
Translation	MSGRARARARGRARGQEPPAPGKEPQPGYTQKTSTEEQAEPEIVGRGRQKRASVAHSTPVTQAGDLQISAGFQEISLGDRGGRRRDFHDLGINTRQAIEHVKESKTGSSGSIIQLSTNHIKLISRPQWVLFQYHIDYAPQMESRGLRCALLFQHEELIGKARAFDGTMLFLPKRLDKVNEVFSQTRNGETVKITITLTNELPPTSPTCFQFYNIIFRRLLKMMNMKQIGRNYYNPNDQIEISSHGLTIYPGFSTSILQYENNIMLSIDVSHKVLRSETVLDYMYNVNQKVESHKFHDICSKDLIGQIVLTKYNNKTYRIDDINWDFTPESTFKKSDGSEISFVDYYRTQYNKGITDLNQPALVHNPKKPRGPQNVPAGPILLIPEFCFLTGLTDRMRSDFNVMKDLAIHTRLAPEQREIQVGKFLKNIHKDDSVQKELQDWGLNFDSKLLPFAGRVAPAEKILQAGKTADYNPQFADWSRELRGPTLIRVKHLDNWVLLYTRRNYDAANTLTQNLFKVSGQMGIKMNRAVMVEVDDTTDAYVKVLQQKVTPDVQMVVCLLSSNRKDKYDAIKKYLCIDCPVPSQCVLAKTLNKPQTVVSVATKIALQMNCKMGGELWTVEIPLKELMIVGIDCYHDTLSGKRSIGAFVASLNPSMTRWFSRCVLQAQKQEIVDGLKVCMHAALKAWFNCNKSLPSRIIIYRDGVGDGQLKTMVEYEIPQLQDCIRSAEKDYSPKLTVIVVKKRVSARFFAHVGGRLQNPPPGTIVDVEITRPEWYDFFIISQSVRVGSVSPTHYNVVYDSGALKPDHMQRLTYKLCHLYYNWPGVIRVPAPCQYAHKLAFLVGQSIHREPHLTLSDRLYYL

Target ORF information:

RefSeq Version	XM_002940181.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis piwi-like RNA-mediated gene silencing 1 (piwil1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002940181.3

ORF Insert Sequence:

ATGTCAGGAA GAGCTAGAGC AAGAGCTCGA GGCAGAGCCC GTGGTCAGGA ACCACCAGCA 
CCTGGAAAGG AGCCTCAACC TGGATATACA CAAAAGACAT CAACAGAAGA GCAAGCAGAA 
CCAGAGATTG TTGGGCGTGG ACGTCAGAAG AGAGCCTCTG TTGCTCACTC AACTCCAGTG 
ACACAGGCAG GAGACCTGCA GATATCAGCA GGATTTCAAG AAATATCTCT TGGGGATCGT 
GGTGGACGTA GACGTGATTT TCATGACCTT GGTATAAATA CTCGTCAAGC AATAGAACAC 
GTCAAAGAGT CAAAAACAGG TTCTTCTGGA AGCATAATTC AGCTAAGTAC AAACCATATA 
AAACTTATCT CTAGACCTCA GTGGGTATTA TTCCAATATC ATATTGACTA TGCCCCACAG 
ATGGAATCTA GGGGATTGCG CTGTGCTTTA CTCTTTCAGC ATGAGGAACT TATTGGAAAA 
GCACGTGCTT TTGATGGAAC AATGTTATTT TTACCTAAAA GACTTGATAA GGTTAATGAG 
GTTTTTAGTC AAACCCGCAA TGGTGAGACA GTGAAGATCA CTATTACTTT AACAAACGAG 
TTACCACCTA CTTCACCAAC GTGCTTCCAG TTCTACAACA TCATTTTTCG AAGACTTCTG 
AAGATGATGA ATATGAAGCA GATTGGACGT AATTACTACA ATCCTAATGA TCAGATAGAA 
ATTAGTTCAC ATGGCCTTAC TATATATCCA GGGTTTTCCA CTTCAATTCT GCAATATGAA 
AACAACATAA TGTTAAGCAT AGATGTGAGC CACAAAGTTT TAAGAAGCGA AACAGTTCTG 
GATTACATGT ACAATGTGAA CCAAAAAGTG GAGTCACATA AATTCCATGA TATCTGCAGC 
AAAGACCTGA TTGGACAAAT TGTTCTTACA AAGTACAACA ATAAGACTTA CCGGATTGAT 
GATATCAACT GGGATTTTAC TCCAGAGTCT ACATTTAAGA AATCAGATGG TTCAGAAATA 
AGCTTTGTTG ATTACTATAG AACACAATAT AATAAAGGAA TCACAGACTT AAACCAGCCT 
GCTCTTGTTC ATAATCCAAA AAAACCCAGG GGTCCACAGA ATGTTCCAGC AGGACCAATT 
CTCTTGATAC CAGAATTTTG TTTCCTAACA GGTTTAACTG ATCGAATGAG GAGTGATTTT 
AATGTAATGA AGGACCTAGC AATTCATACC AGACTGGCAC CAGAGCAAAG AGAAATACAA 
GTTGGAAAAT TTCTAAAGAA TATTCACAAG GATGATAGTG TGCAGAAAGA GCTTCAGGAT 
TGGGGCTTAA ACTTTGACTC AAAACTTTTG CCTTTTGCTG GCAGAGTAGC TCCAGCAGAG 
AAGATTTTGC AGGCTGGAAA AACAGCTGAC TACAATCCTC AGTTTGCTGA CTGGTCAAGA 
GAATTACGAG GACCGACACT AATCCGTGTG AAACATTTGG ATAATTGGGT ATTGTTATAC 
ACACGCAGAA ACTATGATGC TGCCAATACT TTGACACAAA ATCTGTTCAA AGTGTCAGGT 
CAAATGGGAA TCAAGATGAA TAGAGCAGTA ATGGTGGAAG TAGATGACAC TACAGATGCA 
TATGTAAAAG TTTTGCAGCA AAAGGTAACA CCTGATGTAC AGATGGTAGT TTGTCTTCTC 
TCTAGTAATC GGAAGGATAA ATATGATGCT ATAAAAAAAT ACCTGTGCAT TGACTGCCCA 
GTTCCAAGTC AATGCGTGTT AGCAAAGACT TTAAATAAAC CACAGACCGT TGTTTCAGTT 
GCAACAAAAA TTGCATTACA GATGAACTGC AAGATGGGGG GAGAGTTATG GACTGTTGAA 
ATACCTCTGA AGGAATTGAT GATTGTTGGC ATTGATTGTT ATCACGACAC ATTATCAGGA 
AAAAGATCTA TTGGGGCATT TGTTGCCAGT TTAAACCCAA GCATGACACG GTGGTTTTCT 
CGTTGTGTTC TCCAGGCACA GAAACAAGAG ATTGTTGATG GTCTCAAAGT CTGCATGCAT 
GCTGCCCTAA AAGCCTGGTT TAATTGCAAC AAAAGTCTGC CTTCCCGTAT AATAATCTAC 
CGAGATGGAG TAGGAGATGG TCAGTTGAAA ACAATGGTCG AATACGAAAT ACCACAGCTC 
CAGGATTGTA TAAGGTCTGC TGAAAAAGAT TACAGCCCAA AACTAACTGT TATTGTCGTA 
AAAAAACGAG TCAGTGCTAG ATTCTTTGCA CATGTTGGTG GTAGACTTCA GAACCCTCCT 
CCAGGCACCA TTGTTGATGT GGAAATCACG AGACCAGAAT GGTATGACTT CTTCATAATC 
AGCCAGTCTG TAAGAGTAGG TTCTGTGTCG CCAACTCACT ACAACGTTGT GTATGACAGC 
GGCGCATTGA AACCAGATCA TATGCAAAGA CTGACTTACA AGCTTTGCCA TCTGTACTAC 
AACTGGCCAG GTGTAATCAG AGTGCCAGCT CCTTGTCAGT ATGCCCATAA ATTAGCATTT 
TTGGTTGGTC AGAGCATCCA CAGAGAACCA CATCTTACAC TTTCGGATCG CCTATATTAT 
CTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

piwil1 ( XM_018090379.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017945868.1 Xenopus tropicalis piwi-like RNA-mediated gene silencing 1 (piwil1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa34304
Clone ID Related Accession (Same CDS sequence)	XM_018090379.2 , XM_018090379.1
Accession Version	XM_018090379.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2460bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	piwi-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGGAA GAGCTAGAGC AAGAGCTCGA GGCAGAGCCC GTGGTCAGGA ACCACCAGCA 
CCTGGAAAGG AGCCTCAACC TGGATATACA CAAAAGACAT CAACAGAAGA GCAAGCAGAA 
CCAGAGATTG TTGGGCGTGG ACGTCAGAAG AGAGCCTCTG TTGCTCACTC AACTCCAGTG 
ACACAGGCAG GAGACCTGCA GATATCAGCA GGATTTCAAG AAATATCTCT TGGGGATCGT 
GGTGGACGTA GACGTGATTT TCATGACCTT GGTATAAATA CTCGTCAAGC AATAGAACAC 
GTCAAAGAGT CAAAAACAGG TTCTTCTGGA AGCATAATTC AGCTAAGTAC AAACCATATA 
AAACTTATCT CTAGACCTCA GTGGGTATTA TTCCAATATC ATATTGACTA TGCCCCACAG 
ATGGAATCTA GGGGATTGCG CTGTGCTTTA CTCTTTCAGC ATGAGGAACT TATTGGAAAA 
GCACGTGCTT TTGATGGAAC AATGTTATTT TTACCTAAAA GACTTGATAA GGTTAATGAG 
GTTTTTAGTC AAACCCGCAA TGGTGAGACA GTGAAGATCA CTATTACTTT AACAAACGAG 
TTACCACCTA CTTCACCAAC GTGCTTCCAG TTCTACAACA TCATTTTTCG AAGACTTCTG 
AAGATGATGA ATATGAAGCA GATTGGACGT AATTACTACA ATCCTAATGA TCAGATAGAA 
ATTAGTTCAC ATGGCCTTAC TATATATCCA GGGTTTTCCA CTTCAATTCT GCAATATGAA 
AACAACATAA TGTTAAGCAT AGATGTGAGC CACAAAGTTT TAAGAAGCGA AACAGTTCTG 
GATTACATGT ACAATGTGAA CCAAAAAGTG GAGTCACATA AATTCCATGA TATCTGCAGC 
AAAGACCTGA TTGGACAAAT TGTTCTTACA AAGTACAACA ATAAGACTTA CCGGATTGAT 
GATATCAACT GGGATTTTAC TCCAGAGTCT ACATTTAAGA AATCAGATGG TTCAGAAATA 
AGCTTTGTTG ATTACTATAG AACACAATAT AATAAAGGAA TCACAGACTT AAACCAGCCT 
GCTCTTGTTC ATAATCCAAA AAAACCCAGG GGTCCACAGA ATGTTCCAGC AGGACCAATT 
CTCTTGATAC CAGAATTTTG TTTCCTAACA GGTTTAACTG ATCGAATGAG GAGTGATTTT 
AATGTAATGA AGGACCTAGC AATTCATACC AGACTGGCAC CAGAGCAAAG AGAAATACAA 
GTTGGAAAAT TTCTAAAGAA TATTCACAAG GATGATAGTG TGCAGAAAGA GCTTCAGGAT 
TGGGGCTTAA ACTTTGACTC AAAACTTTTG CCTTTTGCTG GCAGAGTAGC TCCAGCAGAG 
AAGATTTTGC AGGCTGGAAA AACAGCTGAC TACAATCCTC AGTTTGCTGA CTGGTCAAGA 
GAATTACGAG GACCGACACT AATCCGTGTG AAACATTTGG ATAATTGGGT ATTGTTATAC 
ACACGCAGAA ACTATGATGC TGCCAATACT TTGACACAAA ATCTGTTCAA AGTGTCAGGT 
CAAATGGGAA TCAAGATGAA TAGAGCAGTA ATGGTGGAAG TAGATGACAC TACAGATGCA 
TATGTAAAAG TTTTGCAGCA AAAGGTAACA CCTGATGTAC AGATGGTAGT TTGTCTTCTC 
TCTAGTAATC GGAAGGATAA ATATGATGCT ATAAAAAAAT ACCTGTGCAT TGACTGCCCA 
GTTCCAAGTC AATGCGTGTT AGCAAAGACT TTAAATAAAC CACAGACCGT TGTTTCAGTT 
GCAACAAAAA TTGCATTACA GATGAACTGC AAGATGGGGG GAGAGTTATG GACTGTTGAA 
ATACCTCTGA AGGAATTGAT GATTGTTGGC ATTGATTGTT ATCACGACAC ATTATCAGGA 
AAAAGATCTA TTGGGGCATT TGTTGCCAGT TTAAACCCAA GCATGACACG GTGGTTTTCT 
CGTTGTGTTC TCCAGGCACA GAAACAAGAG ATTGTTGATG GTCTCAAAGT CTGCATGCAT 
GCTGCCCTAA AAGCCTGGTT TAATTGCAAC AAAAGTCTGC CTTCCCGTAT AATAATCTAC 
CGAGATGGAG TAGGAGATGG TCAGTTGAAA ACAATGGTCG AATACGAAAT ACCACAGCTC 
CAGGATTGTA TAAGGTCTGC TGAAAAAGAT TACAGGTATG ACTTCTTCAT AATCAGCCAG 
TCTGTAAGAG TAGGTTCTGT GTCGCCAACT CACTACAACG TTGTGTATGA CAGCGGCGCA 
TTGAAACCAG ATCATATGCA AAGACTGACT TACAAGCTTT GCCATCTGTA CTACAACTGG 
CCAGGTGTAA TCAGAGTGCC AGCTCCTTGT CAGTATGCCC ATAAATTAGC ATTTTTGGTT 
GGTCAGAGCA TCCACAGAGA ACCACATCTT ACACTTTCGG ATCGCCTATA TTATCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017945868.1
CDS	223..2682
Translation	MSGRARARARGRARGQEPPAPGKEPQPGYTQKTSTEEQAEPEIVGRGRQKRASVAHSTPVTQAGDLQISAGFQEISLGDRGGRRRDFHDLGINTRQAIEHVKESKTGSSGSIIQLSTNHIKLISRPQWVLFQYHIDYAPQMESRGLRCALLFQHEELIGKARAFDGTMLFLPKRLDKVNEVFSQTRNGETVKITITLTNELPPTSPTCFQFYNIIFRRLLKMMNMKQIGRNYYNPNDQIEISSHGLTIYPGFSTSILQYENNIMLSIDVSHKVLRSETVLDYMYNVNQKVESHKFHDICSKDLIGQIVLTKYNNKTYRIDDINWDFTPESTFKKSDGSEISFVDYYRTQYNKGITDLNQPALVHNPKKPRGPQNVPAGPILLIPEFCFLTGLTDRMRSDFNVMKDLAIHTRLAPEQREIQVGKFLKNIHKDDSVQKELQDWGLNFDSKLLPFAGRVAPAEKILQAGKTADYNPQFADWSRELRGPTLIRVKHLDNWVLLYTRRNYDAANTLTQNLFKVSGQMGIKMNRAVMVEVDDTTDAYVKVLQQKVTPDVQMVVCLLSSNRKDKYDAIKKYLCIDCPVPSQCVLAKTLNKPQTVVSVATKIALQMNCKMGGELWTVEIPLKELMIVGIDCYHDTLSGKRSIGAFVASLNPSMTRWFSRCVLQAQKQEIVDGLKVCMHAALKAWFNCNKSLPSRIIIYRDGVGDGQLKTMVEYEIPQLQDCIRSAEKDYRYDFFIISQSVRVGSVSPTHYNVVYDSGALKPDHMQRLTYKLCHLYYNWPGVIRVPAPCQYAHKLAFLVGQSIHREPHLTLSDRLYYL

Target ORF information:

RefSeq Version	XM_018090379.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis piwi-like RNA-mediated gene silencing 1 (piwil1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018090379.1

ORF Insert Sequence:

ATGTCAGGAA GAGCTAGAGC AAGAGCTCGA GGCAGAGCCC GTGGTCAGGA ACCACCAGCA 
CCTGGAAAGG AGCCTCAACC TGGATATACA CAAAAGACAT CAACAGAAGA GCAAGCAGAA 
CCAGAGATTG TTGGGCGTGG ACGTCAGAAG AGAGCCTCTG TTGCTCACTC AACTCCAGTG 
ACACAGGCAG GAGACCTGCA GATATCAGCA GGATTTCAAG AAATATCTCT TGGGGATCGT 
GGTGGACGTA GACGTGATTT TCATGACCTT GGTATAAATA CTCGTCAAGC AATAGAACAC 
GTCAAAGAGT CAAAAACAGG TTCTTCTGGA AGCATAATTC AGCTAAGTAC AAACCATATA 
AAACTTATCT CTAGACCTCA GTGGGTATTA TTCCAATATC ATATTGACTA TGCCCCACAG 
ATGGAATCTA GGGGATTGCG CTGTGCTTTA CTCTTTCAGC ATGAGGAACT TATTGGAAAA 
GCACGTGCTT TTGATGGAAC AATGTTATTT TTACCTAAAA GACTTGATAA GGTTAATGAG 
GTTTTTAGTC AAACCCGCAA TGGTGAGACA GTGAAGATCA CTATTACTTT AACAAACGAG 
TTACCACCTA CTTCACCAAC GTGCTTCCAG TTCTACAACA TCATTTTTCG AAGACTTCTG 
AAGATGATGA ATATGAAGCA GATTGGACGT AATTACTACA ATCCTAATGA TCAGATAGAA 
ATTAGTTCAC ATGGCCTTAC TATATATCCA GGGTTTTCCA CTTCAATTCT GCAATATGAA 
AACAACATAA TGTTAAGCAT AGATGTGAGC CACAAAGTTT TAAGAAGCGA AACAGTTCTG 
GATTACATGT ACAATGTGAA CCAAAAAGTG GAGTCACATA AATTCCATGA TATCTGCAGC 
AAAGACCTGA TTGGACAAAT TGTTCTTACA AAGTACAACA ATAAGACTTA CCGGATTGAT 
GATATCAACT GGGATTTTAC TCCAGAGTCT ACATTTAAGA AATCAGATGG TTCAGAAATA 
AGCTTTGTTG ATTACTATAG AACACAATAT AATAAAGGAA TCACAGACTT AAACCAGCCT 
GCTCTTGTTC ATAATCCAAA AAAACCCAGG GGTCCACAGA ATGTTCCAGC AGGACCAATT 
CTCTTGATAC CAGAATTTTG TTTCCTAACA GGTTTAACTG ATCGAATGAG GAGTGATTTT 
AATGTAATGA AGGACCTAGC AATTCATACC AGACTGGCAC CAGAGCAAAG AGAAATACAA 
GTTGGAAAAT TTCTAAAGAA TATTCACAAG GATGATAGTG TGCAGAAAGA GCTTCAGGAT 
TGGGGCTTAA ACTTTGACTC AAAACTTTTG CCTTTTGCTG GCAGAGTAGC TCCAGCAGAG 
AAGATTTTGC AGGCTGGAAA AACAGCTGAC TACAATCCTC AGTTTGCTGA CTGGTCAAGA 
GAATTACGAG GACCGACACT AATCCGTGTG AAACATTTGG ATAATTGGGT ATTGTTATAC 
ACACGCAGAA ACTATGATGC TGCCAATACT TTGACACAAA ATCTGTTCAA AGTGTCAGGT 
CAAATGGGAA TCAAGATGAA TAGAGCAGTA ATGGTGGAAG TAGATGACAC TACAGATGCA 
TATGTAAAAG TTTTGCAGCA AAAGGTAACA CCTGATGTAC AGATGGTAGT TTGTCTTCTC 
TCTAGTAATC GGAAGGATAA ATATGATGCT ATAAAAAAAT ACCTGTGCAT TGACTGCCCA 
GTTCCAAGTC AATGCGTGTT AGCAAAGACT TTAAATAAAC CACAGACCGT TGTTTCAGTT 
GCAACAAAAA TTGCATTACA GATGAACTGC AAGATGGGGG GAGAGTTATG GACTGTTGAA 
ATACCTCTGA AGGAATTGAT GATTGTTGGC ATTGATTGTT ATCACGACAC ATTATCAGGA 
AAAAGATCTA TTGGGGCATT TGTTGCCAGT TTAAACCCAA GCATGACACG GTGGTTTTCT 
CGTTGTGTTC TCCAGGCACA GAAACAAGAG ATTGTTGATG GTCTCAAAGT CTGCATGCAT 
GCTGCCCTAA AAGCCTGGTT TAATTGCAAC AAAAGTCTGC CTTCCCGTAT AATAATCTAC 
CGAGATGGAG TAGGAGATGG TCAGTTGAAA ACAATGGTCG AATACGAAAT ACCACAGCTC 
CAGGATTGTA TAAGGTCTGC TGAAAAAGAT TACAGGTATG ACTTCTTCAT AATCAGCCAG 
TCTGTAAGAG TAGGTTCTGT GTCGCCAACT CACTACAACG TTGTGTATGA CAGCGGCGCA 
TTGAAACCAG ATCATATGCA AAGACTGACT TACAAGCTTT GCCATCTGTA CTACAACTGG 
CCAGGTGTAA TCAGAGTGCC AGCTCCTTGT CAGTATGCCC ATAAATTAGC ATTTTTGGTT 
GGTCAGAGCA TCCACAGAGA ACCACATCTT ACACTTTCGG ATCGCCTATA TTATCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

piwil1 ( XM_002940181.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002940227.2 Xenopus tropicalis piwi-like RNA-mediated gene silencing 1 (piwil1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa03573
Clone ID Related Accession (Same CDS sequence)	XM_002940181.4 , XM_002940181.3
Accession Version	XM_002940181.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	piwi-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002940181.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGGAA GAGCTAGAGC AAGAGCTCGA GGCAGAGCCC GTGGTCAGGA ACCACCAGCA 
CCTGGAAAGG AGCCTCAACC TGGATATACA CAAAAGACAT CAACAGAAGA GCAAGCAGAA 
CCAGAGATTG TTGGGCGTGG ACGTCAGAAG AGAGCCTCTG TTGCTCACTC AACTCCAGTG 
ACACAGGCAG GAGACCTGCA GATATCAGCA GGATTTCAAG AAATATCTCT TGGGGATCGT 
GGTGGACGTA GACGTGATTT TCATGACCTT GGTATAAATA CTCGTCAAGC AATAGAACAC 
GTCAAAGAGT CAAAAACAGG TTCTTCTGGA AGCATAATTC AGCTAAGTAC AAACCATATA 
AAACTTATCT CTAGACCTCA GTGGGTATTA TTCCAATATC ATATTGACTA TGCCCCACAG 
ATGGAATCTA GGGGATTGCG CTGTGCTTTA CTCTTTCAGC ATGAGGAACT TATTGGAAAA 
GCACGTGCTT TTGATGGAAC AATGTTATTT TTACCTAAAA GACTTGATAA GGTTAATGAG 
GTTTTTAGTC AAACCCGCAA TGGTGAGACA GTGAAGATCA CTATTACTTT AACAAACGAG 
TTACCACCTA CTTCACCAAC GTGCTTCCAG TTCTACAACA TCATTTTTCG AAGACTTCTG 
AAGATGATGA ATATGAAGCA GATTGGACGT AATTACTACA ATCCTAATGA TCAGATAGAA 
ATTAGTTCAC ATGGCCTTAC TATATATCCA GGGTTTTCCA CTTCAATTCT GCAATATGAA 
AACAACATAA TGTTAAGCAT AGATGTGAGC CACAAAGTTT TAAGAAGCGA AACAGTTCTG 
GATTACATGT ACAATGTGAA CCAAAAAGTG GAGTCACATA AATTCCATGA TATCTGCAGC 
AAAGACCTGA TTGGACAAAT TGTTCTTACA AAGTACAACA ATAAGACTTA CCGGATTGAT 
GATATCAACT GGGATTTTAC TCCAGAGTCT ACATTTAAGA AATCAGATGG TTCAGAAATA 
AGCTTTGTTG ATTACTATAG AACACAATAT AATAAAGGAA TCACAGACTT AAACCAGCCT 
GCTCTTGTTC ATAATCCAAA AAAACCCAGG GGTCCACAGA ATGTTCCAGC AGGACCAATT 
CTCTTGATAC CAGAATTTTG TTTCCTAACA GGTTTAACTG ATCGAATGAG GAGTGATTTT 
AATGTAATGA AGGACCTAGC AATTCATACC AGACTGGCAC CAGAGCAAAG AGAAATACAA 
GTTGGAAAAT TTCTAAAGAA TATTCACAAG GATGATAGTG TGCAGAAAGA GCTTCAGGAT 
TGGGGCTTAA ACTTTGACTC AAAACTTTTG CCTTTTGCTG GCAGAGTAGC TCCAGCAGAG 
AAGATTTTGC AGGCTGGAAA AACAGCTGAC TACAATCCTC AGTTTGCTGA CTGGTCAAGA 
GAATTACGAG GACCGACACT AATCCGTGTG AAACATTTGG ATAATTGGGT ATTGTTATAC 
ACACGCAGAA ACTATGATGC TGCCAATACT TTGACACAAA ATCTGTTCAA AGTGTCAGGT 
CAAATGGGAA TCAAGATGAA TAGAGCAGTA ATGGTGGAAG TAGATGACAC TACAGATGCA 
TATGTAAAAG TTTTGCAGCA AAAGGTAACA CCTGATGTAC AGATGGTAGT TTGTCTTCTC 
TCTAGTAATC GGAAGGATAA ATATGATGCT ATAAAAAAAT ACCTGTGCAT TGACTGCCCA 
GTTCCAAGTC AATGCGTGTT AGCAAAGACT TTAAATAAAC CACAGACCGT TGTTTCAGTT 
GCAACAAAAA TTGCATTACA GATGAACTGC AAGATGGGGG GAGAGTTATG GACTGTTGAA 
ATACCTCTGA AGGAATTGAT GATTGTTGGC ATTGATTGTT ATCACGACAC ATTATCAGGA 
AAAAGATCTA TTGGGGCATT TGTTGCCAGT TTAAACCCAA GCATGACACG GTGGTTTTCT 
CGTTGTGTTC TCCAGGCACA GAAACAAGAG ATTGTTGATG GTCTCAAAGT CTGCATGCAT 
GCTGCCCTAA AAGCCTGGTT TAATTGCAAC AAAAGTCTGC CTTCCCGTAT AATAATCTAC 
CGAGATGGAG TAGGAGATGG TCAGTTGAAA ACAATGGTCG AATACGAAAT ACCACAGCTC 
CAGGATTGTA TAAGGTCTGC TGAAAAAGAT TACAGCCCAA AACTAACTGT TATTGTCGTA 
AAAAAACGAG TCAGTGCTAG ATTCTTTGCA CATGTTGGTG GTAGACTTCA GAACCCTCCT 
CCAGGCACCA TTGTTGATGT GGAAATCACG AGACCAGAAT GGTATGACTT CTTCATAATC 
AGCCAGTCTG TAAGAGTAGG TTCTGTGTCG CCAACTCACT ACAACGTTGT GTATGACAGC 
GGCGCATTGA AACCAGATCA TATGCAAAGA CTGACTTACA AGCTTTGCCA TCTGTACTAC 
AACTGGCCAG GTGTAATCAG AGTGCCAGCT CCTTGTCAGT ATGCCCATAA ATTAGCATTT 
TTGGTTGGTC AGAGCATCCA CAGAGAACCA CATCTTACAC TTTCGGATCG CCTATATTAT 
CTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002940227.2
CDS	203..2788
Translation	MSGRARARARGRARGQEPPAPGKEPQPGYTQKTSTEEQAEPEIVGRGRQKRASVAHSTPVTQAGDLQISAGFQEISLGDRGGRRRDFHDLGINTRQAIEHVKESKTGSSGSIIQLSTNHIKLISRPQWVLFQYHIDYAPQMESRGLRCALLFQHEELIGKARAFDGTMLFLPKRLDKVNEVFSQTRNGETVKITITLTNELPPTSPTCFQFYNIIFRRLLKMMNMKQIGRNYYNPNDQIEISSHGLTIYPGFSTSILQYENNIMLSIDVSHKVLRSETVLDYMYNVNQKVESHKFHDICSKDLIGQIVLTKYNNKTYRIDDINWDFTPESTFKKSDGSEISFVDYYRTQYNKGITDLNQPALVHNPKKPRGPQNVPAGPILLIPEFCFLTGLTDRMRSDFNVMKDLAIHTRLAPEQREIQVGKFLKNIHKDDSVQKELQDWGLNFDSKLLPFAGRVAPAEKILQAGKTADYNPQFADWSRELRGPTLIRVKHLDNWVLLYTRRNYDAANTLTQNLFKVSGQMGIKMNRAVMVEVDDTTDAYVKVLQQKVTPDVQMVVCLLSSNRKDKYDAIKKYLCIDCPVPSQCVLAKTLNKPQTVVSVATKIALQMNCKMGGELWTVEIPLKELMIVGIDCYHDTLSGKRSIGAFVASLNPSMTRWFSRCVLQAQKQEIVDGLKVCMHAALKAWFNCNKSLPSRIIIYRDGVGDGQLKTMVEYEIPQLQDCIRSAEKDYSPKLTVIVVKKRVSARFFAHVGGRLQNPPPGTIVDVEITRPEWYDFFIISQSVRVGSVSPTHYNVVYDSGALKPDHMQRLTYKLCHLYYNWPGVIRVPAPCQYAHKLAFLVGQSIHREPHLTLSDRLYYL

Target ORF information:

RefSeq Version	XM_002940181.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis piwi-like RNA-mediated gene silencing 1 (piwil1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002940181.4

ORF Insert Sequence:

ATGTCAGGAA GAGCTAGAGC AAGAGCTCGA GGCAGAGCCC GTGGTCAGGA ACCACCAGCA 
CCTGGAAAGG AGCCTCAACC TGGATATACA CAAAAGACAT CAACAGAAGA GCAAGCAGAA 
CCAGAGATTG TTGGGCGTGG ACGTCAGAAG AGAGCCTCTG TTGCTCACTC AACTCCAGTG 
ACACAGGCAG GAGACCTGCA GATATCAGCA GGATTTCAAG AAATATCTCT TGGGGATCGT 
GGTGGACGTA GACGTGATTT TCATGACCTT GGTATAAATA CTCGTCAAGC AATAGAACAC 
GTCAAAGAGT CAAAAACAGG TTCTTCTGGA AGCATAATTC AGCTAAGTAC AAACCATATA 
AAACTTATCT CTAGACCTCA GTGGGTATTA TTCCAATATC ATATTGACTA TGCCCCACAG 
ATGGAATCTA GGGGATTGCG CTGTGCTTTA CTCTTTCAGC ATGAGGAACT TATTGGAAAA 
GCACGTGCTT TTGATGGAAC AATGTTATTT TTACCTAAAA GACTTGATAA GGTTAATGAG 
GTTTTTAGTC AAACCCGCAA TGGTGAGACA GTGAAGATCA CTATTACTTT AACAAACGAG 
TTACCACCTA CTTCACCAAC GTGCTTCCAG TTCTACAACA TCATTTTTCG AAGACTTCTG 
AAGATGATGA ATATGAAGCA GATTGGACGT AATTACTACA ATCCTAATGA TCAGATAGAA 
ATTAGTTCAC ATGGCCTTAC TATATATCCA GGGTTTTCCA CTTCAATTCT GCAATATGAA 
AACAACATAA TGTTAAGCAT AGATGTGAGC CACAAAGTTT TAAGAAGCGA AACAGTTCTG 
GATTACATGT ACAATGTGAA CCAAAAAGTG GAGTCACATA AATTCCATGA TATCTGCAGC 
AAAGACCTGA TTGGACAAAT TGTTCTTACA AAGTACAACA ATAAGACTTA CCGGATTGAT 
GATATCAACT GGGATTTTAC TCCAGAGTCT ACATTTAAGA AATCAGATGG TTCAGAAATA 
AGCTTTGTTG ATTACTATAG AACACAATAT AATAAAGGAA TCACAGACTT AAACCAGCCT 
GCTCTTGTTC ATAATCCAAA AAAACCCAGG GGTCCACAGA ATGTTCCAGC AGGACCAATT 
CTCTTGATAC CAGAATTTTG TTTCCTAACA GGTTTAACTG ATCGAATGAG GAGTGATTTT 
AATGTAATGA AGGACCTAGC AATTCATACC AGACTGGCAC CAGAGCAAAG AGAAATACAA 
GTTGGAAAAT TTCTAAAGAA TATTCACAAG GATGATAGTG TGCAGAAAGA GCTTCAGGAT 
TGGGGCTTAA ACTTTGACTC AAAACTTTTG CCTTTTGCTG GCAGAGTAGC TCCAGCAGAG 
AAGATTTTGC AGGCTGGAAA AACAGCTGAC TACAATCCTC AGTTTGCTGA CTGGTCAAGA 
GAATTACGAG GACCGACACT AATCCGTGTG AAACATTTGG ATAATTGGGT ATTGTTATAC 
ACACGCAGAA ACTATGATGC TGCCAATACT TTGACACAAA ATCTGTTCAA AGTGTCAGGT 
CAAATGGGAA TCAAGATGAA TAGAGCAGTA ATGGTGGAAG TAGATGACAC TACAGATGCA 
TATGTAAAAG TTTTGCAGCA AAAGGTAACA CCTGATGTAC AGATGGTAGT TTGTCTTCTC 
TCTAGTAATC GGAAGGATAA ATATGATGCT ATAAAAAAAT ACCTGTGCAT TGACTGCCCA 
GTTCCAAGTC AATGCGTGTT AGCAAAGACT TTAAATAAAC CACAGACCGT TGTTTCAGTT 
GCAACAAAAA TTGCATTACA GATGAACTGC AAGATGGGGG GAGAGTTATG GACTGTTGAA 
ATACCTCTGA AGGAATTGAT GATTGTTGGC ATTGATTGTT ATCACGACAC ATTATCAGGA 
AAAAGATCTA TTGGGGCATT TGTTGCCAGT TTAAACCCAA GCATGACACG GTGGTTTTCT 
CGTTGTGTTC TCCAGGCACA GAAACAAGAG ATTGTTGATG GTCTCAAAGT CTGCATGCAT 
GCTGCCCTAA AAGCCTGGTT TAATTGCAAC AAAAGTCTGC CTTCCCGTAT AATAATCTAC 
CGAGATGGAG TAGGAGATGG TCAGTTGAAA ACAATGGTCG AATACGAAAT ACCACAGCTC 
CAGGATTGTA TAAGGTCTGC TGAAAAAGAT TACAGCCCAA AACTAACTGT TATTGTCGTA 
AAAAAACGAG TCAGTGCTAG ATTCTTTGCA CATGTTGGTG GTAGACTTCA GAACCCTCCT 
CCAGGCACCA TTGTTGATGT GGAAATCACG AGACCAGAAT GGTATGACTT CTTCATAATC 
AGCCAGTCTG TAAGAGTAGG TTCTGTGTCG CCAACTCACT ACAACGTTGT GTATGACAGC 
GGCGCATTGA AACCAGATCA TATGCAAAGA CTGACTTACA AGCTTTGCCA TCTGTACTAC 
AACTGGCCAG GTGTAATCAG AGTGCCAGCT CCTTGTCAGT ATGCCCATAA ATTAGCATTT 
TTGGTTGGTC AGAGCATCCA CAGAGAACCA CATCTTACAC TTTCGGATCG CCTATATTAT 
CTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

piwil1 ( XM_018090379.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017945868.1 Xenopus tropicalis piwi-like RNA-mediated gene silencing 1 (piwil1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa34304
Clone ID Related Accession (Same CDS sequence)	XM_018090379.2 , XM_018090379.1
Accession Version	XM_018090379.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2460bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	piwi-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018090379.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGGAA GAGCTAGAGC AAGAGCTCGA GGCAGAGCCC GTGGTCAGGA ACCACCAGCA 
CCTGGAAAGG AGCCTCAACC TGGATATACA CAAAAGACAT CAACAGAAGA GCAAGCAGAA 
CCAGAGATTG TTGGGCGTGG ACGTCAGAAG AGAGCCTCTG TTGCTCACTC AACTCCAGTG 
ACACAGGCAG GAGACCTGCA GATATCAGCA GGATTTCAAG AAATATCTCT TGGGGATCGT 
GGTGGACGTA GACGTGATTT TCATGACCTT GGTATAAATA CTCGTCAAGC AATAGAACAC 
GTCAAAGAGT CAAAAACAGG TTCTTCTGGA AGCATAATTC AGCTAAGTAC AAACCATATA 
AAACTTATCT CTAGACCTCA GTGGGTATTA TTCCAATATC ATATTGACTA TGCCCCACAG 
ATGGAATCTA GGGGATTGCG CTGTGCTTTA CTCTTTCAGC ATGAGGAACT TATTGGAAAA 
GCACGTGCTT TTGATGGAAC AATGTTATTT TTACCTAAAA GACTTGATAA GGTTAATGAG 
GTTTTTAGTC AAACCCGCAA TGGTGAGACA GTGAAGATCA CTATTACTTT AACAAACGAG 
TTACCACCTA CTTCACCAAC GTGCTTCCAG TTCTACAACA TCATTTTTCG AAGACTTCTG 
AAGATGATGA ATATGAAGCA GATTGGACGT AATTACTACA ATCCTAATGA TCAGATAGAA 
ATTAGTTCAC ATGGCCTTAC TATATATCCA GGGTTTTCCA CTTCAATTCT GCAATATGAA 
AACAACATAA TGTTAAGCAT AGATGTGAGC CACAAAGTTT TAAGAAGCGA AACAGTTCTG 
GATTACATGT ACAATGTGAA CCAAAAAGTG GAGTCACATA AATTCCATGA TATCTGCAGC 
AAAGACCTGA TTGGACAAAT TGTTCTTACA AAGTACAACA ATAAGACTTA CCGGATTGAT 
GATATCAACT GGGATTTTAC TCCAGAGTCT ACATTTAAGA AATCAGATGG TTCAGAAATA 
AGCTTTGTTG ATTACTATAG AACACAATAT AATAAAGGAA TCACAGACTT AAACCAGCCT 
GCTCTTGTTC ATAATCCAAA AAAACCCAGG GGTCCACAGA ATGTTCCAGC AGGACCAATT 
CTCTTGATAC CAGAATTTTG TTTCCTAACA GGTTTAACTG ATCGAATGAG GAGTGATTTT 
AATGTAATGA AGGACCTAGC AATTCATACC AGACTGGCAC CAGAGCAAAG AGAAATACAA 
GTTGGAAAAT TTCTAAAGAA TATTCACAAG GATGATAGTG TGCAGAAAGA GCTTCAGGAT 
TGGGGCTTAA ACTTTGACTC AAAACTTTTG CCTTTTGCTG GCAGAGTAGC TCCAGCAGAG 
AAGATTTTGC AGGCTGGAAA AACAGCTGAC TACAATCCTC AGTTTGCTGA CTGGTCAAGA 
GAATTACGAG GACCGACACT AATCCGTGTG AAACATTTGG ATAATTGGGT ATTGTTATAC 
ACACGCAGAA ACTATGATGC TGCCAATACT TTGACACAAA ATCTGTTCAA AGTGTCAGGT 
CAAATGGGAA TCAAGATGAA TAGAGCAGTA ATGGTGGAAG TAGATGACAC TACAGATGCA 
TATGTAAAAG TTTTGCAGCA AAAGGTAACA CCTGATGTAC AGATGGTAGT TTGTCTTCTC 
TCTAGTAATC GGAAGGATAA ATATGATGCT ATAAAAAAAT ACCTGTGCAT TGACTGCCCA 
GTTCCAAGTC AATGCGTGTT AGCAAAGACT TTAAATAAAC CACAGACCGT TGTTTCAGTT 
GCAACAAAAA TTGCATTACA GATGAACTGC AAGATGGGGG GAGAGTTATG GACTGTTGAA 
ATACCTCTGA AGGAATTGAT GATTGTTGGC ATTGATTGTT ATCACGACAC ATTATCAGGA 
AAAAGATCTA TTGGGGCATT TGTTGCCAGT TTAAACCCAA GCATGACACG GTGGTTTTCT 
CGTTGTGTTC TCCAGGCACA GAAACAAGAG ATTGTTGATG GTCTCAAAGT CTGCATGCAT 
GCTGCCCTAA AAGCCTGGTT TAATTGCAAC AAAAGTCTGC CTTCCCGTAT AATAATCTAC 
CGAGATGGAG TAGGAGATGG TCAGTTGAAA ACAATGGTCG AATACGAAAT ACCACAGCTC 
CAGGATTGTA TAAGGTCTGC TGAAAAAGAT TACAGGTATG ACTTCTTCAT AATCAGCCAG 
TCTGTAAGAG TAGGTTCTGT GTCGCCAACT CACTACAACG TTGTGTATGA CAGCGGCGCA 
TTGAAACCAG ATCATATGCA AAGACTGACT TACAAGCTTT GCCATCTGTA CTACAACTGG 
CCAGGTGTAA TCAGAGTGCC AGCTCCTTGT CAGTATGCCC ATAAATTAGC ATTTTTGGTT 
GGTCAGAGCA TCCACAGAGA ACCACATCTT ACACTTTCGG ATCGCCTATA TTATCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017945868.1
CDS	204..2663
Translation	MSGRARARARGRARGQEPPAPGKEPQPGYTQKTSTEEQAEPEIVGRGRQKRASVAHSTPVTQAGDLQISAGFQEISLGDRGGRRRDFHDLGINTRQAIEHVKESKTGSSGSIIQLSTNHIKLISRPQWVLFQYHIDYAPQMESRGLRCALLFQHEELIGKARAFDGTMLFLPKRLDKVNEVFSQTRNGETVKITITLTNELPPTSPTCFQFYNIIFRRLLKMMNMKQIGRNYYNPNDQIEISSHGLTIYPGFSTSILQYENNIMLSIDVSHKVLRSETVLDYMYNVNQKVESHKFHDICSKDLIGQIVLTKYNNKTYRIDDINWDFTPESTFKKSDGSEISFVDYYRTQYNKGITDLNQPALVHNPKKPRGPQNVPAGPILLIPEFCFLTGLTDRMRSDFNVMKDLAIHTRLAPEQREIQVGKFLKNIHKDDSVQKELQDWGLNFDSKLLPFAGRVAPAEKILQAGKTADYNPQFADWSRELRGPTLIRVKHLDNWVLLYTRRNYDAANTLTQNLFKVSGQMGIKMNRAVMVEVDDTTDAYVKVLQQKVTPDVQMVVCLLSSNRKDKYDAIKKYLCIDCPVPSQCVLAKTLNKPQTVVSVATKIALQMNCKMGGELWTVEIPLKELMIVGIDCYHDTLSGKRSIGAFVASLNPSMTRWFSRCVLQAQKQEIVDGLKVCMHAALKAWFNCNKSLPSRIIIYRDGVGDGQLKTMVEYEIPQLQDCIRSAEKDYRYDFFIISQSVRVGSVSPTHYNVVYDSGALKPDHMQRLTYKLCHLYYNWPGVIRVPAPCQYAHKLAFLVGQSIHREPHLTLSDRLYYL

Target ORF information:

RefSeq Version	XM_018090379.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis piwi-like RNA-mediated gene silencing 1 (piwil1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018090379.2

ORF Insert Sequence:

ATGTCAGGAA GAGCTAGAGC AAGAGCTCGA GGCAGAGCCC GTGGTCAGGA ACCACCAGCA 
CCTGGAAAGG AGCCTCAACC TGGATATACA CAAAAGACAT CAACAGAAGA GCAAGCAGAA 
CCAGAGATTG TTGGGCGTGG ACGTCAGAAG AGAGCCTCTG TTGCTCACTC AACTCCAGTG 
ACACAGGCAG GAGACCTGCA GATATCAGCA GGATTTCAAG AAATATCTCT TGGGGATCGT 
GGTGGACGTA GACGTGATTT TCATGACCTT GGTATAAATA CTCGTCAAGC AATAGAACAC 
GTCAAAGAGT CAAAAACAGG TTCTTCTGGA AGCATAATTC AGCTAAGTAC AAACCATATA 
AAACTTATCT CTAGACCTCA GTGGGTATTA TTCCAATATC ATATTGACTA TGCCCCACAG 
ATGGAATCTA GGGGATTGCG CTGTGCTTTA CTCTTTCAGC ATGAGGAACT TATTGGAAAA 
GCACGTGCTT TTGATGGAAC AATGTTATTT TTACCTAAAA GACTTGATAA GGTTAATGAG 
GTTTTTAGTC AAACCCGCAA TGGTGAGACA GTGAAGATCA CTATTACTTT AACAAACGAG 
TTACCACCTA CTTCACCAAC GTGCTTCCAG TTCTACAACA TCATTTTTCG AAGACTTCTG 
AAGATGATGA ATATGAAGCA GATTGGACGT AATTACTACA ATCCTAATGA TCAGATAGAA 
ATTAGTTCAC ATGGCCTTAC TATATATCCA GGGTTTTCCA CTTCAATTCT GCAATATGAA 
AACAACATAA TGTTAAGCAT AGATGTGAGC CACAAAGTTT TAAGAAGCGA AACAGTTCTG 
GATTACATGT ACAATGTGAA CCAAAAAGTG GAGTCACATA AATTCCATGA TATCTGCAGC 
AAAGACCTGA TTGGACAAAT TGTTCTTACA AAGTACAACA ATAAGACTTA CCGGATTGAT 
GATATCAACT GGGATTTTAC TCCAGAGTCT ACATTTAAGA AATCAGATGG TTCAGAAATA 
AGCTTTGTTG ATTACTATAG AACACAATAT AATAAAGGAA TCACAGACTT AAACCAGCCT 
GCTCTTGTTC ATAATCCAAA AAAACCCAGG GGTCCACAGA ATGTTCCAGC AGGACCAATT 
CTCTTGATAC CAGAATTTTG TTTCCTAACA GGTTTAACTG ATCGAATGAG GAGTGATTTT 
AATGTAATGA AGGACCTAGC AATTCATACC AGACTGGCAC CAGAGCAAAG AGAAATACAA 
GTTGGAAAAT TTCTAAAGAA TATTCACAAG GATGATAGTG TGCAGAAAGA GCTTCAGGAT 
TGGGGCTTAA ACTTTGACTC AAAACTTTTG CCTTTTGCTG GCAGAGTAGC TCCAGCAGAG 
AAGATTTTGC AGGCTGGAAA AACAGCTGAC TACAATCCTC AGTTTGCTGA CTGGTCAAGA 
GAATTACGAG GACCGACACT AATCCGTGTG AAACATTTGG ATAATTGGGT ATTGTTATAC 
ACACGCAGAA ACTATGATGC TGCCAATACT TTGACACAAA ATCTGTTCAA AGTGTCAGGT 
CAAATGGGAA TCAAGATGAA TAGAGCAGTA ATGGTGGAAG TAGATGACAC TACAGATGCA 
TATGTAAAAG TTTTGCAGCA AAAGGTAACA CCTGATGTAC AGATGGTAGT TTGTCTTCTC 
TCTAGTAATC GGAAGGATAA ATATGATGCT ATAAAAAAAT ACCTGTGCAT TGACTGCCCA 
GTTCCAAGTC AATGCGTGTT AGCAAAGACT TTAAATAAAC CACAGACCGT TGTTTCAGTT 
GCAACAAAAA TTGCATTACA GATGAACTGC AAGATGGGGG GAGAGTTATG GACTGTTGAA 
ATACCTCTGA AGGAATTGAT GATTGTTGGC ATTGATTGTT ATCACGACAC ATTATCAGGA 
AAAAGATCTA TTGGGGCATT TGTTGCCAGT TTAAACCCAA GCATGACACG GTGGTTTTCT 
CGTTGTGTTC TCCAGGCACA GAAACAAGAG ATTGTTGATG GTCTCAAAGT CTGCATGCAT 
GCTGCCCTAA AAGCCTGGTT TAATTGCAAC AAAAGTCTGC CTTCCCGTAT AATAATCTAC 
CGAGATGGAG TAGGAGATGG TCAGTTGAAA ACAATGGTCG AATACGAAAT ACCACAGCTC 
CAGGATTGTA TAAGGTCTGC TGAAAAAGAT TACAGGTATG ACTTCTTCAT AATCAGCCAG 
TCTGTAAGAG TAGGTTCTGT GTCGCCAACT CACTACAACG TTGTGTATGA CAGCGGCGCA 
TTGAAACCAG ATCATATGCA AAGACTGACT TACAAGCTTT GCCATCTGTA CTACAACTGG 
CCAGGTGTAA TCAGAGTGCC AGCTCCTTGT CAGTATGCCC ATAAATTAGC ATTTTTGGTT 
GGTCAGAGCA TCCACAGAGA ACCACATCTT ACACTTTCGG ATCGCCTATA TTATCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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piwil1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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