ddx59 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ddx59
Entrez Gene ID	100488709
Full Name	DEAD-box helicase 59
Synonyms	centroid
General protein information	Preferred Names DEAD-box helicase 59 Names probable ATP-dependent RNA helicase DDX59 DEAD (Asp-Glu-Ala-Asp) box polypeptide 59
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031901039.1	XP_031756899.1	probable ATP-dependent RNA helicase DDX59 isoform X3
XM_002932152.4	XP_002932198.2	probable ATP-dependent RNA helicase DDX59 isoform X1
XM_012962500.1	XP_012817954.1	probable ATP-dependent RNA helicase DDX59 isoform X2
XM_018093631.1	XP_017949120.1	probable ATP-dependent RNA helicase DDX59 isoform X3
XM_031901037.1	XP_031756897.1	probable ATP-dependent RNA helicase DDX59 isoform X1
XM_031901038.1	XP_031756898.1	probable ATP-dependent RNA helicase DDX59 isoform X2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ddx59	NP_080776.1
Danio rerio (zebrafish)	ddx59	NP_001038443.2
Bos taurus (cattle)	DDX59	NP_001179318.1
Xenopus tropicalis (tropical clawed frog)	ddx59	XP_002932198.2
Pan troglodytes (chimpanzee)	DDX59	XP_001143546.1
	LOC709192	XP_001109688.2
Canis lupus familiaris (dog)	DDX59	XP_005622331.1
Gallus gallus (chicken)	DDX59	XP_004943161.1
Arabidopsis thaliana (thale cress)	AT3G02065	NP_001030621.1
Homo sapiens (human)	DDX59	NP_001026895.2
Rattus norvegicus (Norway rat)	Ddx59	NP_001177749.1

Gene Ontology

The following ddx59 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ddx59 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa57325	XM_031901037.1 Latest version!	Xenopus tropicalis DEAD-box helicase 59 (ddx59), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa57326	XM_031901038.1 Latest version!	Xenopus tropicalis DEAD-box helicase 59 (ddx59), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa57327	XM_031901039.1 Latest version!	Xenopus tropicalis DEAD-box helicase 59 (ddx59), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa09114	XM_002932152.4 Latest version!	Xenopus tropicalis DEAD-box helicase 59 (ddx59), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OXa34295	XM_012962500.1 Latest version!	Xenopus tropicalis DEAD-box helicase 59 (ddx59), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OXa44204	XM_018093631.1 Latest version!	Xenopus tropicalis DEAD-box helicase 59 (ddx59), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ddx59 ( XM_031901037.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756897.1 Xenopus tropicalis DEAD-box helicase 59 (ddx59), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa57325
Clone ID Related Accession (Same CDS sequence)	XM_031901037.1
Accession Version	XM_031901037.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2358bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	probable ATP-dependent RNA helicase DDX59 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCTGC CCCGAGCATT GAAACGGCGG CTGGAAGTGA AGACTACAGT GGGGAAGAGA 
GCCCCTCTGC TAAAGGACCA GTCCGCTACA CCCGCTCCTG TGCCCTCCGC GCAGCAATCC 
CCATACTGTT CTACAGCGGA GTCTGTGCCT GATCCGACTC TGCAAGTGTC CCTTTCTTCT 
CCGACCCCAC CGGCATCATC TGTAGCTGCC TCTGCTTTTA CTTCTCTTAT GCCAGCTGTT 
AGTCCTGCTG CCCCATCTCT GCTCACTACT GAGGCTTCGT CTTTACACAC TTCTGCTTCG 
CCCATCACAC CTGCACCCAC CGCTACCCCT TCCCTTACAC CTGCACCCAC CGCTACCCCT 
TCCCTTACAC CTGCACCCAC CGCTACCCCT TCCCTTACAC CTGCACCCAC CGCTACCCCT 
TCCCTTACAC CTGCACCCAC CGCTACCCCT TCCCTTACAC CTGCACCTAC CGCTACCCCT 
TCCCTTACAC CTGCACCCAC CTCCACTCAA TTCCTCAAGG CTGCAAGCTC TTCTACAACA 
CCCACACCAT CCGTACCCAC CTCTGCTCCA TCCATATCAT GTCCTTCCAT CCCTGCTTCT 
TTCTCACAAC CTATCAAACT CCCACCATTA TCCAAATCTT CAGAGGATGC CCATGAAGAA 
GCAGTTAAGT CATACAGTAA ATGTCAGCGC TGGCCGTTTC CTGGGGATCC TGTATGTGTA 
ATCTGTGGAA GATATGGAGA GTATATATGT GACCAGACGG ACCATGATGT GTGCAGCTTA 
GAGTGCAAAG CCATGGACAT TTTGCGGGTT TGTGGAGCAT CCAGCACCCC ATGTGCTATC 
ACAGACATTA CCTTAAGCCC ACAGCCTTTA TTGTATTCAG CTATTGGGCC TCTGGATAAT 
TCAGCCATCA CTTCTACAGT CTCCACTGAA CTGACCTCAT GCCAAAATAG TGATGCAGAT 
ACTTCACATG TGACCGCATA CAACCACCCA ATGCCTTATA TCTACAAGGA ACATGAGTTC 
ATCTCACAGT TAAGCGCTGA CCAGATAAAC CACCTGAGAC AGCAGCTTAG CTTGTCAGTA 
CATGGGAATG AGATGTGCAA ACCTATCATG GAGTTTGACC ACTGTCAGTT TCCTCCTGTG 
CTCAGCTCTA ACATAAAGGC AGCAGGTTAT GAAGTGCCTA CTCCCATACA GATGCAGATG 
ATCCCAGTGG GGCTTATGGG AAGAGATATA TTGGCAAGTG CAGATACTGG CTCAGGGAAA 
ACTGCAGCTT TTCTTCTTCC AGCTATAATT CGATGTCTTG AAAAGAAGGA TTCTCCAGCT 
GCACTGATTC TTACACCCAC TAGAGAACTG GCTGTGCAGA TAGAGGGACA GGCCAAAGAA 
CTGATGCGTG GGATTCCTCA CATGAGGACT GCACTACTTG TGGGCGGGAT GCCTCTGCCC 
CCTCAGATAC ACCGTCTGAA ACAAGGTGTA CAGGTTATAA TAGCAACTCC TGGGAGACTT 
CTAGAAATCA TTAAGCAGGA TTCTGTGAAT CTTGGTGATC TAAAGATTCT TATTGTGGAT 
GAGGCAGATA CCATGCTGAA GATGGGGTTT CAGCAGCAAG TCCTAGATAT TTTGGAGGTT 
GTACCACACG ACCATCAGAC CATTCTGGTT TCAGCTACCA TCCCAGCTGG CATTGAGGCC 
TTCACAAAGC AGCTCCTGCA GGATCCTGTG CGAATCACTG TAGGCGAGAA AAACCAGCCC 
TGCACCAACG TGAGACAGAT TGTGCTGTGG GTGGAGGAAC CTTCAAAGAA GAAAAAGCTC 
TTTGAAATTT TGAATGATTC CAAGCTTTTC CAGCCCCCCG TCTTAGTGTT TGTGGATTGC 
CGTCTCGGTG CTGATCTGCT GAGTGATGCC GTCAGTAAGA TCACAGGGTT AGAGTGTGTG 
GCAATGCACT CTGATAAATC CCAGGTGGAA CGAATGAAGA TCTTGCAGGG TTTGCTCCAG 
GGAGAATATG ATGTTGTGGT GAGCACTGGA GTTCTGGGGC GTGGGCTGGA TCTGGTGAAT 
GTCAAGTTGG TGGTGAATTT TGACATGCCA TCGAGTATGG ATGAGTATGT GCACCAGATT 
GGCAGAGCAG GAAGGCTTGG CCACAGGGGA ACAGCTATAA CTTTCATCAA CAAGAACAAC 
CGCAACCTCT TCTGGGACCT GGTGAAGAGA GTGCAGCCCA CTGGCTCTTT GCTGCCCCCG 
CAGCTGCTAA ATTCCCCCTA TGTACAAGAA CAGAAAAAAG TTGACGAAAG ATTGCGGCGG 
GACAAAGAGA AGGTGGTAAC GGGAGACCAA ATTCTTGATC TTATTCGAAA ACATGACAGG 
CATAAATCTC ATAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756897.1
CDS	259..2616
Translation	MFLPRALKRRLEVKTTVGKRAPLLKDQSATPAPVPSAQQSPYCSTAESVPDPTLQVSLSSPTPPASSVAASAFTSLMPAVSPAAPSLLTTEASSLHTSASPITPAPTATPSLTPAPTATPSLTPAPTATPSLTPAPTATPSLTPAPTATPSLTPAPTATPSLTPAPTSTQFLKAASSSTTPTPSVPTSAPSISCPSIPASFSQPIKLPPLSKSSEDAHEEAVKSYSKCQRWPFPGDPVCVICGRYGEYICDQTDHDVCSLECKAMDILRVCGASSTPCAITDITLSPQPLLYSAIGPLDNSAITSTVSTELTSCQNSDADTSHVTAYNHPMPYIYKEHEFISQLSADQINHLRQQLSLSVHGNEMCKPIMEFDHCQFPPVLSSNIKAAGYEVPTPIQMQMIPVGLMGRDILASADTGSGKTAAFLLPAIIRCLEKKDSPAALILTPTRELAVQIEGQAKELMRGIPHMRTALLVGGMPLPPQIHRLKQGVQVIIATPGRLLEIIKQDSVNLGDLKILIVDEADTMLKMGFQQQVLDILEVVPHDHQTILVSATIPAGIEAFTKQLLQDPVRITVGEKNQPCTNVRQIVLWVEEPSKKKKLFEILNDSKLFQPPVLVFVDCRLGADLLSDAVSKITGLECVAMHSDKSQVERMKILQGLLQGEYDVVVSTGVLGRGLDLVNVKLVVNFDMPSSMDEYVHQIGRAGRLGHRGTAITFINKNNRNLFWDLVKRVQPTGSLLPPQLLNSPYVQEQKKVDERLRRDKEKVVTGDQILDLIRKHDRHKSHK

Target ORF information:

RefSeq Version	XM_031901037.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DEAD-box helicase 59 (ddx59), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901037.1

ORF Insert Sequence:

ATGTTCCTGC CCCGAGCATT GAAACGGCGG CTGGAAGTGA AGACTACAGT GGGGAAGAGA 
GCCCCTCTGC TAAAGGACCA GTCCGCTACA CCCGCTCCTG TGCCCTCCGC GCAGCAATCC 
CCATACTGTT CTACAGCGGA GTCTGTGCCT GATCCGACTC TGCAAGTGTC CCTTTCTTCT 
CCGACCCCAC CGGCATCATC TGTAGCTGCC TCTGCTTTTA CTTCTCTTAT GCCAGCTGTT 
AGTCCTGCTG CCCCATCTCT GCTCACTACT GAGGCTTCGT CTTTACACAC TTCTGCTTCG 
CCCATCACAC CTGCACCCAC CGCTACCCCT TCCCTTACAC CTGCACCCAC CGCTACCCCT 
TCCCTTACAC CTGCACCCAC CGCTACCCCT TCCCTTACAC CTGCACCCAC CGCTACCCCT 
TCCCTTACAC CTGCACCCAC CGCTACCCCT TCCCTTACAC CTGCACCTAC CGCTACCCCT 
TCCCTTACAC CTGCACCCAC CTCCACTCAA TTCCTCAAGG CTGCAAGCTC TTCTACAACA 
CCCACACCAT CCGTACCCAC CTCTGCTCCA TCCATATCAT GTCCTTCCAT CCCTGCTTCT 
TTCTCACAAC CTATCAAACT CCCACCATTA TCCAAATCTT CAGAGGATGC CCATGAAGAA 
GCAGTTAAGT CATACAGTAA ATGTCAGCGC TGGCCGTTTC CTGGGGATCC TGTATGTGTA 
ATCTGTGGAA GATATGGAGA GTATATATGT GACCAGACGG ACCATGATGT GTGCAGCTTA 
GAGTGCAAAG CCATGGACAT TTTGCGGGTT TGTGGAGCAT CCAGCACCCC ATGTGCTATC 
ACAGACATTA CCTTAAGCCC ACAGCCTTTA TTGTATTCAG CTATTGGGCC TCTGGATAAT 
TCAGCCATCA CTTCTACAGT CTCCACTGAA CTGACCTCAT GCCAAAATAG TGATGCAGAT 
ACTTCACATG TGACCGCATA CAACCACCCA ATGCCTTATA TCTACAAGGA ACATGAGTTC 
ATCTCACAGT TAAGCGCTGA CCAGATAAAC CACCTGAGAC AGCAGCTTAG CTTGTCAGTA 
CATGGGAATG AGATGTGCAA ACCTATCATG GAGTTTGACC ACTGTCAGTT TCCTCCTGTG 
CTCAGCTCTA ACATAAAGGC AGCAGGTTAT GAAGTGCCTA CTCCCATACA GATGCAGATG 
ATCCCAGTGG GGCTTATGGG AAGAGATATA TTGGCAAGTG CAGATACTGG CTCAGGGAAA 
ACTGCAGCTT TTCTTCTTCC AGCTATAATT CGATGTCTTG AAAAGAAGGA TTCTCCAGCT 
GCACTGATTC TTACACCCAC TAGAGAACTG GCTGTGCAGA TAGAGGGACA GGCCAAAGAA 
CTGATGCGTG GGATTCCTCA CATGAGGACT GCACTACTTG TGGGCGGGAT GCCTCTGCCC 
CCTCAGATAC ACCGTCTGAA ACAAGGTGTA CAGGTTATAA TAGCAACTCC TGGGAGACTT 
CTAGAAATCA TTAAGCAGGA TTCTGTGAAT CTTGGTGATC TAAAGATTCT TATTGTGGAT 
GAGGCAGATA CCATGCTGAA GATGGGGTTT CAGCAGCAAG TCCTAGATAT TTTGGAGGTT 
GTACCACACG ACCATCAGAC CATTCTGGTT TCAGCTACCA TCCCAGCTGG CATTGAGGCC 
TTCACAAAGC AGCTCCTGCA GGATCCTGTG CGAATCACTG TAGGCGAGAA AAACCAGCCC 
TGCACCAACG TGAGACAGAT TGTGCTGTGG GTGGAGGAAC CTTCAAAGAA GAAAAAGCTC 
TTTGAAATTT TGAATGATTC CAAGCTTTTC CAGCCCCCCG TCTTAGTGTT TGTGGATTGC 
CGTCTCGGTG CTGATCTGCT GAGTGATGCC GTCAGTAAGA TCACAGGGTT AGAGTGTGTG 
GCAATGCACT CTGATAAATC CCAGGTGGAA CGAATGAAGA TCTTGCAGGG TTTGCTCCAG 
GGAGAATATG ATGTTGTGGT GAGCACTGGA GTTCTGGGGC GTGGGCTGGA TCTGGTGAAT 
GTCAAGTTGG TGGTGAATTT TGACATGCCA TCGAGTATGG ATGAGTATGT GCACCAGATT 
GGCAGAGCAG GAAGGCTTGG CCACAGGGGA ACAGCTATAA CTTTCATCAA CAAGAACAAC 
CGCAACCTCT TCTGGGACCT GGTGAAGAGA GTGCAGCCCA CTGGCTCTTT GCTGCCCCCG 
CAGCTGCTAA ATTCCCCCTA TGTACAAGAA CAGAAAAAAG TTGACGAAAG ATTGCGGCGG 
GACAAAGAGA AGGTGGTAAC GGGAGACCAA ATTCTTGATC TTATTCGAAA ACATGACAGG 
CATAAATCTC ATAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ddx59 ( XM_031901038.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756898.1 Xenopus tropicalis DEAD-box helicase 59 (ddx59), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa57326
Clone ID Related Accession (Same CDS sequence)	XM_031901038.1
Accession Version	XM_031901038.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	probable ATP-dependent RNA helicase DDX59 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCTGC CCCGAGCATT GAAACGGCGG CTGGAAGTGA AGACTACAGT GGGGAAGAGA 
GCCCCTCTGC TAAAGGACCA GTCCGCTACA CCCGCTCCTG TGCCCTCCGC GCAGCAATCC 
CCATACTGTT CTACAGCGGA GTCTGTGCCT GATCCGACTC TGCAAGTGTC CCTTTCTTCT 
CCGACCCCAC CGGCATCATC TGTAGCTGCC TCTGCTTTTA CTTCTCTTAT GCCAGCTGTT 
AGTCCTGCTG CCCCATCTCT GCTCACTACT GAGGCTTCGT CTTTACACAC TTCTGCTTCG 
CCCATCACAC CTGCACCCAC CGCTACCCCT TCCCTTACAC CTGCACCCAC CGCTACCCCT 
TCCCTTACAC CTGCACCCAC CGCTACCCCT TCCCTTACAC CTGCACCCAC CGCTACCCCT 
TCCCTTACAC CTGCACCCAC CGCTACCCCT TCCCTTACAC CTGCACCTAC CGCTACCCCT 
TCCCTTACAC CTGCACCCAC CTCCACTCAA TTCCTCAAGG CTGCAAGCTC TTCTACAACA 
CCCACACCAT CCGTACCCAC CTCTGCTCCA TCCATATCAT GTCCTTCCAT CCCTGCTTCT 
TTCTCACAAC CTATCAAACT CCCACCATTA TCCAAATCTT CAGAGGATGC CCATGAAGAA 
GCAGTTAAGT CATACAGTAA ATGTCAGCGC TGGCCGTTTC CTGGGGATCC TGTATGTGTA 
ATCTGTGGAA GATATGGAGA GTATATATGT GACCAGACGG ACCATGATGT GTGCAGCTTA 
GAGTGCAAAG CCATGGACAT TTTGCGGGTT TGTGGAGCAT CCAGCACCCC ATGTGCTATC 
ACAGACATTA CCTTAAGCCC ACAGCCTTTA TTGTATTCAG CTATTGGGCC TCTGGATAAT 
TCAGCCATCA CTTCTACAGT CTCCACTGAA CTGACCTCAT GCCAAAATAG TGATGCAGAT 
ACTTCACATG TGACCGCATA CAACCACCCA ATGCCTTATA TCTACAAGGA ACATGAGTTC 
ATCTCACAGT TAAGCGCTGA CCAGATAAAC CACCTGAGAC AGCAGCTTAG CTTGTCAGTA 
CATGGGAATG AGATGTGCAA ACCTATCATG GAGTTTGACC ACTGTCAGTT TCCTCCTGTG 
CTCAGCTCTA ACATAAAGGC AGCAGGTTAT GAAGTGCCTA CTCCCATACA GATGCAGATG 
ATCCCAGTGG GGCTTATGGG AAGAGATATA TTGGCAAGTG CAGATACTGG CTCAGGGAAA 
ACTGCAGCTT TTCTTCTTCC AGCTATAATT CGATGTCTTG AAAAGGATTC TCCAGCTGCA 
CTGATTCTTA CACCCACTAG AGAACTGGCT GTGCAGATAG AGGGACAGGC CAAAGAACTG 
ATGCGTGGGA TTCCTCACAT GAGGACTGCA CTACTTGTGG GCGGGATGCC TCTGCCCCCT 
CAGATACACC GTCTGAAACA AGGTGTACAG GTTATAATAG CAACTCCTGG GAGACTTCTA 
GAAATCATTA AGCAGGATTC TGTGAATCTT GGTGATCTAA AGATTCTTAT TGTGGATGAG 
GCAGATACCA TGCTGAAGAT GGGGTTTCAG CAGCAAGTCC TAGATATTTT GGAGGTTGTA 
CCACACGACC ATCAGACCAT TCTGGTTTCA GCTACCATCC CAGCTGGCAT TGAGGCCTTC 
ACAAAGCAGC TCCTGCAGGA TCCTGTGCGA ATCACTGTAG GCGAGAAAAA CCAGCCCTGC 
ACCAACGTGA GACAGATTGT GCTGTGGGTG GAGGAACCTT CAAAGAAGAA AAAGCTCTTT 
GAAATTTTGA ATGATTCCAA GCTTTTCCAG CCCCCCGTCT TAGTGTTTGT GGATTGCCGT 
CTCGGTGCTG ATCTGCTGAG TGATGCCGTC AGTAAGATCA CAGGGTTAGA GTGTGTGGCA 
ATGCACTCTG ATAAATCCCA GGTGGAACGA ATGAAGATCT TGCAGGGTTT GCTCCAGGGA 
GAATATGATG TTGTGGTGAG CACTGGAGTT CTGGGGCGTG GGCTGGATCT GGTGAATGTC 
AAGTTGGTGG TGAATTTTGA CATGCCATCG AGTATGGATG AGTATGTGCA CCAGATTGGC 
AGAGCAGGAA GGCTTGGCCA CAGGGGAACA GCTATAACTT TCATCAACAA GAACAACCGC 
AACCTCTTCT GGGACCTGGT GAAGAGAGTG CAGCCCACTG GCTCTTTGCT GCCCCCGCAG 
CTGCTAAATT CCCCCTATGT ACAAGAACAG AAAAAAGTTG ACGAAAGATT GCGGCGGGAC 
AAAGAGAAGG TGGTAACGGG AGACCAAATT CTTGATCTTA TTCGAAAACA TGACAGGCAT 
AAATCTCATA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756898.1
CDS	260..2614
Translation	MFLPRALKRRLEVKTTVGKRAPLLKDQSATPAPVPSAQQSPYCSTAESVPDPTLQVSLSSPTPPASSVAASAFTSLMPAVSPAAPSLLTTEASSLHTSASPITPAPTATPSLTPAPTATPSLTPAPTATPSLTPAPTATPSLTPAPTATPSLTPAPTATPSLTPAPTSTQFLKAASSSTTPTPSVPTSAPSISCPSIPASFSQPIKLPPLSKSSEDAHEEAVKSYSKCQRWPFPGDPVCVICGRYGEYICDQTDHDVCSLECKAMDILRVCGASSTPCAITDITLSPQPLLYSAIGPLDNSAITSTVSTELTSCQNSDADTSHVTAYNHPMPYIYKEHEFISQLSADQINHLRQQLSLSVHGNEMCKPIMEFDHCQFPPVLSSNIKAAGYEVPTPIQMQMIPVGLMGRDILASADTGSGKTAAFLLPAIIRCLEKDSPAALILTPTRELAVQIEGQAKELMRGIPHMRTALLVGGMPLPPQIHRLKQGVQVIIATPGRLLEIIKQDSVNLGDLKILIVDEADTMLKMGFQQQVLDILEVVPHDHQTILVSATIPAGIEAFTKQLLQDPVRITVGEKNQPCTNVRQIVLWVEEPSKKKKLFEILNDSKLFQPPVLVFVDCRLGADLLSDAVSKITGLECVAMHSDKSQVERMKILQGLLQGEYDVVVSTGVLGRGLDLVNVKLVVNFDMPSSMDEYVHQIGRAGRLGHRGTAITFINKNNRNLFWDLVKRVQPTGSLLPPQLLNSPYVQEQKKVDERLRRDKEKVVTGDQILDLIRKHDRHKSHK

Target ORF information:

RefSeq Version	XM_031901038.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DEAD-box helicase 59 (ddx59), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901038.1

ORF Insert Sequence:

ATGTTCCTGC CCCGAGCATT GAAACGGCGG CTGGAAGTGA AGACTACAGT GGGGAAGAGA 
GCCCCTCTGC TAAAGGACCA GTCCGCTACA CCCGCTCCTG TGCCCTCCGC GCAGCAATCC 
CCATACTGTT CTACAGCGGA GTCTGTGCCT GATCCGACTC TGCAAGTGTC CCTTTCTTCT 
CCGACCCCAC CGGCATCATC TGTAGCTGCC TCTGCTTTTA CTTCTCTTAT GCCAGCTGTT 
AGTCCTGCTG CCCCATCTCT GCTCACTACT GAGGCTTCGT CTTTACACAC TTCTGCTTCG 
CCCATCACAC CTGCACCCAC CGCTACCCCT TCCCTTACAC CTGCACCCAC CGCTACCCCT 
TCCCTTACAC CTGCACCCAC CGCTACCCCT TCCCTTACAC CTGCACCCAC CGCTACCCCT 
TCCCTTACAC CTGCACCCAC CGCTACCCCT TCCCTTACAC CTGCACCTAC CGCTACCCCT 
TCCCTTACAC CTGCACCCAC CTCCACTCAA TTCCTCAAGG CTGCAAGCTC TTCTACAACA 
CCCACACCAT CCGTACCCAC CTCTGCTCCA TCCATATCAT GTCCTTCCAT CCCTGCTTCT 
TTCTCACAAC CTATCAAACT CCCACCATTA TCCAAATCTT CAGAGGATGC CCATGAAGAA 
GCAGTTAAGT CATACAGTAA ATGTCAGCGC TGGCCGTTTC CTGGGGATCC TGTATGTGTA 
ATCTGTGGAA GATATGGAGA GTATATATGT GACCAGACGG ACCATGATGT GTGCAGCTTA 
GAGTGCAAAG CCATGGACAT TTTGCGGGTT TGTGGAGCAT CCAGCACCCC ATGTGCTATC 
ACAGACATTA CCTTAAGCCC ACAGCCTTTA TTGTATTCAG CTATTGGGCC TCTGGATAAT 
TCAGCCATCA CTTCTACAGT CTCCACTGAA CTGACCTCAT GCCAAAATAG TGATGCAGAT 
ACTTCACATG TGACCGCATA CAACCACCCA ATGCCTTATA TCTACAAGGA ACATGAGTTC 
ATCTCACAGT TAAGCGCTGA CCAGATAAAC CACCTGAGAC AGCAGCTTAG CTTGTCAGTA 
CATGGGAATG AGATGTGCAA ACCTATCATG GAGTTTGACC ACTGTCAGTT TCCTCCTGTG 
CTCAGCTCTA ACATAAAGGC AGCAGGTTAT GAAGTGCCTA CTCCCATACA GATGCAGATG 
ATCCCAGTGG GGCTTATGGG AAGAGATATA TTGGCAAGTG CAGATACTGG CTCAGGGAAA 
ACTGCAGCTT TTCTTCTTCC AGCTATAATT CGATGTCTTG AAAAGGATTC TCCAGCTGCA 
CTGATTCTTA CACCCACTAG AGAACTGGCT GTGCAGATAG AGGGACAGGC CAAAGAACTG 
ATGCGTGGGA TTCCTCACAT GAGGACTGCA CTACTTGTGG GCGGGATGCC TCTGCCCCCT 
CAGATACACC GTCTGAAACA AGGTGTACAG GTTATAATAG CAACTCCTGG GAGACTTCTA 
GAAATCATTA AGCAGGATTC TGTGAATCTT GGTGATCTAA AGATTCTTAT TGTGGATGAG 
GCAGATACCA TGCTGAAGAT GGGGTTTCAG CAGCAAGTCC TAGATATTTT GGAGGTTGTA 
CCACACGACC ATCAGACCAT TCTGGTTTCA GCTACCATCC CAGCTGGCAT TGAGGCCTTC 
ACAAAGCAGC TCCTGCAGGA TCCTGTGCGA ATCACTGTAG GCGAGAAAAA CCAGCCCTGC 
ACCAACGTGA GACAGATTGT GCTGTGGGTG GAGGAACCTT CAAAGAAGAA AAAGCTCTTT 
GAAATTTTGA ATGATTCCAA GCTTTTCCAG CCCCCCGTCT TAGTGTTTGT GGATTGCCGT 
CTCGGTGCTG ATCTGCTGAG TGATGCCGTC AGTAAGATCA CAGGGTTAGA GTGTGTGGCA 
ATGCACTCTG ATAAATCCCA GGTGGAACGA ATGAAGATCT TGCAGGGTTT GCTCCAGGGA 
GAATATGATG TTGTGGTGAG CACTGGAGTT CTGGGGCGTG GGCTGGATCT GGTGAATGTC 
AAGTTGGTGG TGAATTTTGA CATGCCATCG AGTATGGATG AGTATGTGCA CCAGATTGGC 
AGAGCAGGAA GGCTTGGCCA CAGGGGAACA GCTATAACTT TCATCAACAA GAACAACCGC 
AACCTCTTCT GGGACCTGGT GAAGAGAGTG CAGCCCACTG GCTCTTTGCT GCCCCCGCAG 
CTGCTAAATT CCCCCTATGT ACAAGAACAG AAAAAAGTTG ACGAAAGATT GCGGCGGGAC 
AAAGAGAAGG TGGTAACGGG AGACCAAATT CTTGATCTTA TTCGAAAACA TGACAGGCAT 
AAATCTCATA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ddx59 ( XM_031901039.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756899.1 Xenopus tropicalis DEAD-box helicase 59 (ddx59), transcript variant X3, mRNA.

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CloneID	OXa57327
Clone ID Related Accession (Same CDS sequence)	XM_031901039.1
Accession Version	XM_031901039.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2229bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	probable ATP-dependent RNA helicase DDX59 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCTGC CCCGAGCATT GAAACGGCGG CTGGAAGTGA AGACTACAGT GGGGAAGAGA 
GCCCCTCTGC TAAAGGACCA GTCCGCTACA CCCGCTCCTG TGCCCTCCGC GCAGCAATCC 
CCATACTGTT CTACAGCGGA GTCTGTGCCT GATCCGACTC TGCAAGTGTC CCTTTCTTCT 
CCGACCCCAC CGGCATCATC TGTAGCTGCC TCTGCTTTTA CTTCTCTTAT GCCAGCTGTT 
AGTCCTGCTG CCCCATCTCT GCTCACTACT GAGGCTTCGT CTTTACACAC TTCTGCTTCG 
CCCATCACAC CTGCACCCAC CGCTACCCCT TCCCTTACAC CTGCACCCAC CGCTACCCCT 
TCCCTTACAC CTGCACCCAC CGCTACCCCT TCCCTTACAC CTGCACCCAC CGCTACCCCT 
TCCCTTACAC CTGCACCCAC CGCTACCCCT TCCCTTACAC CTGCACCTAC CGCTACCCCT 
TCCCTTACAC CTGCACCCAC CTCCACTCAA TTCCTCAAGG CTGCAAGCTC TTCTACAACA 
CCCACACCAT CCGTACCCAC CTCTGCTCCA TCCATATCAT GTCCTTCCAT CCCTGCTTCT 
TTCTCACAAC CTATCAAACT CCCACCATTA TCCAAATCTT CAGAGGATGC CCATGAAGAA 
GCAGTTAAGT CATACAGTAA ATGTCAGCGC TGGCCGTTTC CTGGGGATCC TGTATGTGTA 
ATCTGTGGAA GATATGGAGA GTATATATGT GACCAGACGG ACCATGATGT GTGCAGCTTA 
GAGTGCAAAG CCATGGACAT TTTGCGGGTT TGTGGAGCAT CCAGCACCCC ATGTGCTATC 
ACAGACATTA CCTTAAGCCC ACAGCCTTTA TTGTATTCAG CTATTGGGCC TCTGGATAAT 
TCAGCCATCA CTTCTACAGT CTCCACTGAA CTGACCTCAT GCCAAAATAG TGATGCAGAT 
ACTTCACATG TGACCGCATA CAACCACCCA ATGCCTTATA TCTACAAGGA ACATGAGTTC 
ATCTCACAGT TAAGCGCTGA CCAGATAAAC CACCTGAGAC AGCAGCTTAG CTTGTCAGTA 
CATGGGAATG AGATGTGCAA ACCTATCATG GAGTTTGACC ACTGTCAGTT TCCTCCTGTG 
CTCAGCTCTA ACATAAAGGC AGCAGGTTAT GAAGTGCCTA CTCCCATACA GATGCAGATG 
ATCCCAGTGG GGCTTATGGG AAGAGATATA TTGGCAAGTG CAGATACTGG CTCAGGGAAA 
ACTGCAGCTT TTCTTCTTCC AGCTATAATT CGATGTCTTG AAAAGAAGGA TTCTCCAGCT 
GCACTGATTC TTACACCCAC TAGAGAACTG GCTGTGCAGA TAGAGGGACA GGCCAAAGAA 
CTGATGCGTG GGATTCCTCA CATGAGGACT GCACTACTTG TGGGCGGGAT GCCTCTGCCC 
CCTCAGATAC ACCGTCTGAA ACAAGGTGTA CAGGTTATAA TAGCAACTCC TGGGAGACTT 
CTAGAAATCA TTAAGCAGGA TTCTGTGAAT CTTGGTGATC TAAAGATTCT TATTGTGGAT 
GAGGCAGATA CCATGCTGAA GATGGGGTTT CAGCAGCAAG TCCTAGATAT TTTGGAGGTT 
GTACCACACG ACCATCAGAC CATTCTGGTT TCAGCTACCA TCCCAGCTGG CATTGAGGCC 
TTCACAAAGC AGCTCCTGCA GGATCCTGTG CGAATCACTG TAGGCGAGAA AAACCAGCCC 
TGCACCAACG TGAGACAGAT TGTGCTGTGG GTGGAGGAAC CTTCAAAGAA GAAAAAGCTC 
TTTGAAATTT TGAATGATTC CAAGCTTTTC CAGCCCCCCG TCTTAGTGTT TGTGGATTGC 
CGTCTCGGTG CTGATCTGCT GAGTGATGCC GTCAGTAAGA TCACAGGGTT AGAGTGTGTG 
GCAATGCACT CTGATAAATC CCAGGTGGAA CGAATGAAGA TCTTGCAGAT TGGCAGAGCA 
GGAAGGCTTG GCCACAGGGG AACAGCTATA ACTTTCATCA ACAAGAACAA CCGCAACCTC 
TTCTGGGACC TGGTGAAGAG AGTGCAGCCC ACTGGCTCTT TGCTGCCCCC GCAGCTGCTA 
AATTCCCCCT ATGTACAAGA ACAGAAAAAA GTTGACGAAA GATTGCGGCG GGACAAAGAG 
AAGGTGGTAA CGGGAGACCA AATTCTTGAT CTTATTCGAA AACATGACAG GCATAAATCT 
CATAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756899.1
CDS	261..2489
Translation	MFLPRALKRRLEVKTTVGKRAPLLKDQSATPAPVPSAQQSPYCSTAESVPDPTLQVSLSSPTPPASSVAASAFTSLMPAVSPAAPSLLTTEASSLHTSASPITPAPTATPSLTPAPTATPSLTPAPTATPSLTPAPTATPSLTPAPTATPSLTPAPTATPSLTPAPTSTQFLKAASSSTTPTPSVPTSAPSISCPSIPASFSQPIKLPPLSKSSEDAHEEAVKSYSKCQRWPFPGDPVCVICGRYGEYICDQTDHDVCSLECKAMDILRVCGASSTPCAITDITLSPQPLLYSAIGPLDNSAITSTVSTELTSCQNSDADTSHVTAYNHPMPYIYKEHEFISQLSADQINHLRQQLSLSVHGNEMCKPIMEFDHCQFPPVLSSNIKAAGYEVPTPIQMQMIPVGLMGRDILASADTGSGKTAAFLLPAIIRCLEKKDSPAALILTPTRELAVQIEGQAKELMRGIPHMRTALLVGGMPLPPQIHRLKQGVQVIIATPGRLLEIIKQDSVNLGDLKILIVDEADTMLKMGFQQQVLDILEVVPHDHQTILVSATIPAGIEAFTKQLLQDPVRITVGEKNQPCTNVRQIVLWVEEPSKKKKLFEILNDSKLFQPPVLVFVDCRLGADLLSDAVSKITGLECVAMHSDKSQVERMKILQIGRAGRLGHRGTAITFINKNNRNLFWDLVKRVQPTGSLLPPQLLNSPYVQEQKKVDERLRRDKEKVVTGDQILDLIRKHDRHKSHK

Target ORF information:

RefSeq Version	XM_031901039.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DEAD-box helicase 59 (ddx59), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901039.1

ORF Insert Sequence:

ATGTTCCTGC CCCGAGCATT GAAACGGCGG CTGGAAGTGA AGACTACAGT GGGGAAGAGA 
GCCCCTCTGC TAAAGGACCA GTCCGCTACA CCCGCTCCTG TGCCCTCCGC GCAGCAATCC 
CCATACTGTT CTACAGCGGA GTCTGTGCCT GATCCGACTC TGCAAGTGTC CCTTTCTTCT 
CCGACCCCAC CGGCATCATC TGTAGCTGCC TCTGCTTTTA CTTCTCTTAT GCCAGCTGTT 
AGTCCTGCTG CCCCATCTCT GCTCACTACT GAGGCTTCGT CTTTACACAC TTCTGCTTCG 
CCCATCACAC CTGCACCCAC CGCTACCCCT TCCCTTACAC CTGCACCCAC CGCTACCCCT 
TCCCTTACAC CTGCACCCAC CGCTACCCCT TCCCTTACAC CTGCACCCAC CGCTACCCCT 
TCCCTTACAC CTGCACCCAC CGCTACCCCT TCCCTTACAC CTGCACCTAC CGCTACCCCT 
TCCCTTACAC CTGCACCCAC CTCCACTCAA TTCCTCAAGG CTGCAAGCTC TTCTACAACA 
CCCACACCAT CCGTACCCAC CTCTGCTCCA TCCATATCAT GTCCTTCCAT CCCTGCTTCT 
TTCTCACAAC CTATCAAACT CCCACCATTA TCCAAATCTT CAGAGGATGC CCATGAAGAA 
GCAGTTAAGT CATACAGTAA ATGTCAGCGC TGGCCGTTTC CTGGGGATCC TGTATGTGTA 
ATCTGTGGAA GATATGGAGA GTATATATGT GACCAGACGG ACCATGATGT GTGCAGCTTA 
GAGTGCAAAG CCATGGACAT TTTGCGGGTT TGTGGAGCAT CCAGCACCCC ATGTGCTATC 
ACAGACATTA CCTTAAGCCC ACAGCCTTTA TTGTATTCAG CTATTGGGCC TCTGGATAAT 
TCAGCCATCA CTTCTACAGT CTCCACTGAA CTGACCTCAT GCCAAAATAG TGATGCAGAT 
ACTTCACATG TGACCGCATA CAACCACCCA ATGCCTTATA TCTACAAGGA ACATGAGTTC 
ATCTCACAGT TAAGCGCTGA CCAGATAAAC CACCTGAGAC AGCAGCTTAG CTTGTCAGTA 
CATGGGAATG AGATGTGCAA ACCTATCATG GAGTTTGACC ACTGTCAGTT TCCTCCTGTG 
CTCAGCTCTA ACATAAAGGC AGCAGGTTAT GAAGTGCCTA CTCCCATACA GATGCAGATG 
ATCCCAGTGG GGCTTATGGG AAGAGATATA TTGGCAAGTG CAGATACTGG CTCAGGGAAA 
ACTGCAGCTT TTCTTCTTCC AGCTATAATT CGATGTCTTG AAAAGAAGGA TTCTCCAGCT 
GCACTGATTC TTACACCCAC TAGAGAACTG GCTGTGCAGA TAGAGGGACA GGCCAAAGAA 
CTGATGCGTG GGATTCCTCA CATGAGGACT GCACTACTTG TGGGCGGGAT GCCTCTGCCC 
CCTCAGATAC ACCGTCTGAA ACAAGGTGTA CAGGTTATAA TAGCAACTCC TGGGAGACTT 
CTAGAAATCA TTAAGCAGGA TTCTGTGAAT CTTGGTGATC TAAAGATTCT TATTGTGGAT 
GAGGCAGATA CCATGCTGAA GATGGGGTTT CAGCAGCAAG TCCTAGATAT TTTGGAGGTT 
GTACCACACG ACCATCAGAC CATTCTGGTT TCAGCTACCA TCCCAGCTGG CATTGAGGCC 
TTCACAAAGC AGCTCCTGCA GGATCCTGTG CGAATCACTG TAGGCGAGAA AAACCAGCCC 
TGCACCAACG TGAGACAGAT TGTGCTGTGG GTGGAGGAAC CTTCAAAGAA GAAAAAGCTC 
TTTGAAATTT TGAATGATTC CAAGCTTTTC CAGCCCCCCG TCTTAGTGTT TGTGGATTGC 
CGTCTCGGTG CTGATCTGCT GAGTGATGCC GTCAGTAAGA TCACAGGGTT AGAGTGTGTG 
GCAATGCACT CTGATAAATC CCAGGTGGAA CGAATGAAGA TCTTGCAGAT TGGCAGAGCA 
GGAAGGCTTG GCCACAGGGG AACAGCTATA ACTTTCATCA ACAAGAACAA CCGCAACCTC 
TTCTGGGACC TGGTGAAGAG AGTGCAGCCC ACTGGCTCTT TGCTGCCCCC GCAGCTGCTA 
AATTCCCCCT ATGTACAAGA ACAGAAAAAA GTTGACGAAA GATTGCGGCG GGACAAAGAG 
AAGGTGGTAA CGGGAGACCA AATTCTTGAT CTTATTCGAA AACATGACAG GCATAAATCT 
CATAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ddx59 ( XM_002932152.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002932198.2 Xenopus tropicalis DEAD-box helicase 59 (ddx59), transcript variant X1, mRNA.

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CloneID	OXa09114
Clone ID Related Accession (Same CDS sequence)	XM_002932152.4
Accession Version	XM_002932152.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	probable ATP-dependent RNA helicase DDX59 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002932152.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGCTG TTAGTCCTGC TGCCCCATCT CTGCTCACTA CTGAGGCTTC GTCTTTACAC 
ACTTCTGCTT CGCCCATCAC ACCTGCACCC ACCGCTACCC CTTCCCTTAC ACCTGCACCC 
ACCGCTACCC CTTCCCTTAC ACCTGCACCC ACCGCTACCC CTTCCCTTAC ACCTGCACCC 
ACCGCTACCC CTTCCCTTAC ACCTGCACCC ACCTCCACTC AATTCCTCAA GGCTGCAAGC 
TCTTCTACAA CACCCACACC ATCCGTACCC ACCTCTGCTC CATCCATATC ATGTCCTTCC 
ATCCCTGCTT CTTTCTCACA ACCTATCAAA CTCCCACCAT TATCCAAATC TTCAGAGGAT 
GCCCATGAAG AAGCAGTTAA GTCATACAGT AAATGTCAGC GCTGGCCGTT TCCTGGGGAT 
CCTGTATGTG TAATCTGTGG AAGATATGGA GAGTATATAT GTGACCAGAC GGACCATGAT 
GTGTGCAGCT TAGAGTGCAA AGCCATGGAC ATTTTGCGGG TTTGTGGAGC ATCCAGCACC 
CCATGTGCTA TCACAGACAT TACCTTAAGC CCACAGCCTT TATTGTATTC AGCTATTGGG 
CCTCTGGATA ATTCAGCCAT CACTTCTACA GTCTCCACTG AACTGACCTC ATGCCAAAAT 
AGTGATGCAG ATACTTCACA TGTGACCGCA TACAACCACC CAATGCCTTA TATCTACAAG 
GAACATGAGT TCATCTCACA GTTAAGCGCT GACCAGATAA ACCACCTGAG ACAGCAGCTT 
AGCTTGTCAG TACATGGGAA TGAGATGTGC AAACCTATCA TGGAGTTTGA CCACTGTCAG 
TTTCCTCCTG TGCTCAGCTC TAACATAAAG GCAGCAGGTT ATGAAGTGCC TACTCCCATA 
CAGATGCAGA TGATCCCAGT GGGGCTTATG GGAAGAGATA TATTGGCAAG TGCAGATACT 
GGCTCAGGGA AAACTGCAGC TTTTCTTCTT CCAGCTATAA TTCGATGTCT TGAAAAGAAG 
GATTCTCCAG CTGCACTGAT TCTTACACCC ACTAGAGAAC TGGCTGTGCA GATAGAGGGA 
CAGGCCAAAG AACTGATGCG TGGGATTCCT CACATGAGGA CTGCACTACT TGTGGGCGGG 
ATGCCTCTGC CCCCTCAGAT ACACCGTCTG AAACAAGGTG TACAGGTTAT AATAGCAACT 
CCTGGGAGAC TTCTAGAAAT CATTAAGCAG GATTCTGTGA ATCTTGGTGA TCTAAAGATT 
CTTATTGTGG ATGAGGCAGA TACCATGCTG AAGATGGGGT TTCAGCAGCA AGTCCTAGAT 
ATTTTGGAGG TTGTACCACA CGACCATCAG ACCATTCTGG TTTCAGCTAC CATCCCAGCT 
GGCATTGAGG CCTTCACAAA GCAGCTCCTG CAGGATCCTG TGCGAATCAC TGTAGGCGAG 
AAAAACCAGC CCTGCACCAA CGTGAGGCAG ATTGTGCTGT GGGTGGAGGA ACCTTCAAAG 
AAGAAAAAGC TCTTTGAAAT TTTGAATGAT TCCAAGCTTT TCCAGCCCCC CGTCTTAGTG 
TTTGTGGATT GCCGTCTCGG TGCTGATCTG CTGAGTGATG CCGTCAGTAA AATCACAGGG 
TTAGAGTGTG TGGCAATGCA CTCTGATAAA TCCCAGGTGG AACGAATGAA GATCTTGCAG 
GGTTTGCTCC AGGGAGAATA TGATGTTGTG GTGAGCACTG GAGTTCTGGG GCGTGGGCTG 
GATCTGGTGA ATGTCAAGTT GGTGGTGAAT TTTGACATGC CATCGAGTAT GGATGAGTAT 
GTGCACCAGA TTGGCAGAGC AGGAAGGCTT GGCCACAGGG GAACAGCTAT AACTTTCATC 
AACAAGAACA ACCGCAACCT CTTCTGGGAC CTGGTGAAGA GAGTGCAGCC CACTGGCTCT 
TTGCTGCCCC CGCAGCTGCT AAATTCCCCC TATGTACAAG AACAGAAAAA AGTTGACGAA 
AGATTGCGGC GGGACAAAGA GAAGGTGGTA ACGGGAGACC AAATTCTTGA TCTTATTCGA 
AAACATGACA GGCATAAATC TCATAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002932198.2
CDS	17..2086
Translation	MPAVSPAAPSLLTTEASSLHTSASPITPAPTATPSLTPAPTATPSLTPAPTATPSLTPAPTATPSLTPAPTSTQFLKAASSSTTPTPSVPTSAPSISCPSIPASFSQPIKLPPLSKSSEDAHEEAVKSYSKCQRWPFPGDPVCVICGRYGEYICDQTDHDVCSLECKAMDILRVCGASSTPCAITDITLSPQPLLYSAIGPLDNSAITSTVSTELTSCQNSDADTSHVTAYNHPMPYIYKEHEFISQLSADQINHLRQQLSLSVHGNEMCKPIMEFDHCQFPPVLSSNIKAAGYEVPTPIQMQMIPVGLMGRDILASADTGSGKTAAFLLPAIIRCLEKKDSPAALILTPTRELAVQIEGQAKELMRGIPHMRTALLVGGMPLPPQIHRLKQGVQVIIATPGRLLEIIKQDSVNLGDLKILIVDEADTMLKMGFQQQVLDILEVVPHDHQTILVSATIPAGIEAFTKQLLQDPVRITVGEKNQPCTNVRQIVLWVEEPSKKKKLFEILNDSKLFQPPVLVFVDCRLGADLLSDAVSKITGLECVAMHSDKSQVERMKILQGLLQGEYDVVVSTGVLGRGLDLVNVKLVVNFDMPSSMDEYVHQIGRAGRLGHRGTAITFINKNNRNLFWDLVKRVQPTGSLLPPQLLNSPYVQEQKKVDERLRRDKEKVVTGDQILDLIRKHDRHKSHK

Target ORF information:

RefSeq Version	XM_002932152.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DEAD-box helicase 59 (ddx59), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002932152.4

ORF Insert Sequence:

ATGCCAGCTG TTAGTCCTGC TGCCCCATCT CTGCTCACTA CTGAGGCTTC GTCTTTACAC 
ACTTCTGCTT CGCCCATCAC ACCTGCACCC ACCGCTACCC CTTCCCTTAC ACCTGCACCC 
ACCGCTACCC CTTCCCTTAC ACCTGCACCC ACCGCTACCC CTTCCCTTAC ACCTGCACCC 
ACCGCTACCC CTTCCCTTAC ACCTGCACCC ACCTCCACTC AATTCCTCAA GGCTGCAAGC 
TCTTCTACAA CACCCACACC ATCCGTACCC ACCTCTGCTC CATCCATATC ATGTCCTTCC 
ATCCCTGCTT CTTTCTCACA ACCTATCAAA CTCCCACCAT TATCCAAATC TTCAGAGGAT 
GCCCATGAAG AAGCAGTTAA GTCATACAGT AAATGTCAGC GCTGGCCGTT TCCTGGGGAT 
CCTGTATGTG TAATCTGTGG AAGATATGGA GAGTATATAT GTGACCAGAC GGACCATGAT 
GTGTGCAGCT TAGAGTGCAA AGCCATGGAC ATTTTGCGGG TTTGTGGAGC ATCCAGCACC 
CCATGTGCTA TCACAGACAT TACCTTAAGC CCACAGCCTT TATTGTATTC AGCTATTGGG 
CCTCTGGATA ATTCAGCCAT CACTTCTACA GTCTCCACTG AACTGACCTC ATGCCAAAAT 
AGTGATGCAG ATACTTCACA TGTGACCGCA TACAACCACC CAATGCCTTA TATCTACAAG 
GAACATGAGT TCATCTCACA GTTAAGCGCT GACCAGATAA ACCACCTGAG ACAGCAGCTT 
AGCTTGTCAG TACATGGGAA TGAGATGTGC AAACCTATCA TGGAGTTTGA CCACTGTCAG 
TTTCCTCCTG TGCTCAGCTC TAACATAAAG GCAGCAGGTT ATGAAGTGCC TACTCCCATA 
CAGATGCAGA TGATCCCAGT GGGGCTTATG GGAAGAGATA TATTGGCAAG TGCAGATACT 
GGCTCAGGGA AAACTGCAGC TTTTCTTCTT CCAGCTATAA TTCGATGTCT TGAAAAGAAG 
GATTCTCCAG CTGCACTGAT TCTTACACCC ACTAGAGAAC TGGCTGTGCA GATAGAGGGA 
CAGGCCAAAG AACTGATGCG TGGGATTCCT CACATGAGGA CTGCACTACT TGTGGGCGGG 
ATGCCTCTGC CCCCTCAGAT ACACCGTCTG AAACAAGGTG TACAGGTTAT AATAGCAACT 
CCTGGGAGAC TTCTAGAAAT CATTAAGCAG GATTCTGTGA ATCTTGGTGA TCTAAAGATT 
CTTATTGTGG ATGAGGCAGA TACCATGCTG AAGATGGGGT TTCAGCAGCA AGTCCTAGAT 
ATTTTGGAGG TTGTACCACA CGACCATCAG ACCATTCTGG TTTCAGCTAC CATCCCAGCT 
GGCATTGAGG CCTTCACAAA GCAGCTCCTG CAGGATCCTG TGCGAATCAC TGTAGGCGAG 
AAAAACCAGC CCTGCACCAA CGTGAGGCAG ATTGTGCTGT GGGTGGAGGA ACCTTCAAAG 
AAGAAAAAGC TCTTTGAAAT TTTGAATGAT TCCAAGCTTT TCCAGCCCCC CGTCTTAGTG 
TTTGTGGATT GCCGTCTCGG TGCTGATCTG CTGAGTGATG CCGTCAGTAA AATCACAGGG 
TTAGAGTGTG TGGCAATGCA CTCTGATAAA TCCCAGGTGG AACGAATGAA GATCTTGCAG 
GGTTTGCTCC AGGGAGAATA TGATGTTGTG GTGAGCACTG GAGTTCTGGG GCGTGGGCTG 
GATCTGGTGA ATGTCAAGTT GGTGGTGAAT TTTGACATGC CATCGAGTAT GGATGAGTAT 
GTGCACCAGA TTGGCAGAGC AGGAAGGCTT GGCCACAGGG GAACAGCTAT AACTTTCATC 
AACAAGAACA ACCGCAACCT CTTCTGGGAC CTGGTGAAGA GAGTGCAGCC CACTGGCTCT 
TTGCTGCCCC CGCAGCTGCT AAATTCCCCC TATGTACAAG AACAGAAAAA AGTTGACGAA 
AGATTGCGGC GGGACAAAGA GAAGGTGGTA ACGGGAGACC AAATTCTTGA TCTTATTCGA 
AAACATGACA GGCATAAATC TCATAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ddx59 ( XM_012962500.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817954.1 Xenopus tropicalis DEAD-box helicase 59 (ddx59), transcript variant X2, mRNA.

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CloneID	OXa34295
Clone ID Related Accession (Same CDS sequence)	XM_012962500.1
Accession Version	XM_012962500.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	probable ATP-dependent RNA helicase DDX59 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGCTG TTAGTCCTGC TGCCCCATCT CTGCTCACTA CTGAGGCTTC GTCTTTACAC 
ACTTCTGCTT CGCCCATCAC ACCTGCACCC ACCGCTACCC CTTCCCTTAC ACCTGCACCC 
ACCGCTACCC CTTCCCTTAC ACCTGCACCC ACCGCTACCC CTTCCCTTAC ACCTGCACCC 
ACCGCTACCC CTTCCCTTAC ACCTGCACCC ACCTCCACTC AATTCCTCAA GGCTGCAAGC 
TCTTCTACAA CACCCACACC ATCCGTACCC ACCTCTGCTC CATCCATATC ATGTCCTTCC 
ATCCCTGCTT CTTTCTCACA ACCTATCAAA CTCCCACCAT TATCCAAATC TTCAGAGGAT 
GCCCATGAAG AAGCAGTTAA GTCATACAGT AAATGTCAGC GCTGGCCGTT TCCTGGGGAT 
CCTGTATGTG TAATCTGTGG AAGATATGGA GAGTATATAT GTGACCAGAC GGACCATGAT 
GTGTGCAGCT TAGAGTGCAA AGCCATGGAC ATTTTGCGGG TTTGTGGAGC ATCCAGCACC 
CCATGTGCTA TCACAGACAT TACCTTAAGC CCACAGCCTT TATTGTATTC AGCTATTGGG 
CCTCTGGATA ATTCAGCCAT CACTTCTACA GTCTCCACTG AACTGACCTC ATGCCAAAAT 
AGTGATGCAG ATACTTCACA TGTGACCGCA TACAACCACC CAATGCCTTA TATCTACAAG 
GAACATGAGT TCATCTCACA GTTAAGCGCT GACCAGATAA ACCACCTGAG ACAGCAGCTT 
AGCTTGTCAG TACATGGGAA TGAGATGTGC AAACCTATCA TGGAGTTTGA CCACTGTCAG 
TTTCCTCCTG TGCTCAGCTC TAACATAAAG GCAGCAGGTT ATGAAGTGCC TACTCCCATA 
CAGATGCAGA TGATCCCAGT GGGGCTTATG GGAAGAGATA TATTGGCAAG TGCAGATACT 
GGCTCAGGGA AAACTGCAGC TTTTCTTCTT CCAGCTATAA TTCGATGTCT TGAAAAGGAT 
TCTCCAGCTG CACTGATTCT TACACCCACT AGAGAACTGG CTGTGCAGAT AGAGGGACAG 
GCCAAAGAAC TGATGCGTGG GATTCCTCAC ATGAGGACTG CACTACTTGT GGGCGGGATG 
CCTCTGCCCC CTCAGATACA CCGTCTGAAA CAAGGTGTAC AGGTTATAAT AGCAACTCCT 
GGGAGACTTC TAGAAATCAT TAAGCAGGAT TCTGTGAATC TTGGTGATCT AAAGATTCTT 
ATTGTGGATG AGGCAGATAC CATGCTGAAG ATGGGGTTTC AGCAGCAAGT CCTAGATATT 
TTGGAGGTTG TACCACACGA CCATCAGACC ATTCTGGTTT CAGCTACCAT CCCAGCTGGC 
ATTGAGGCCT TCACAAAGCA GCTCCTGCAG GATCCTGTGC GAATCACTGT AGGCGAGAAA 
AACCAGCCCT GCACCAACGT GAGGCAGATT GTGCTGTGGG TGGAGGAACC TTCAAAGAAG 
AAAAAGCTCT TTGAAATTTT GAATGATTCC AAGCTTTTCC AGCCCCCCGT CTTAGTGTTT 
GTGGATTGCC GTCTCGGTGC TGATCTGCTG AGTGATGCCG TCAGTAAAAT CACAGGGTTA 
GAGTGTGTGG CAATGCACTC TGATAAATCC CAGGTGGAAC GAATGAAGAT CTTGCAGGGT 
TTGCTCCAGG GAGAATATGA TGTTGTGGTG AGCACTGGAG TTCTGGGGCG TGGGCTGGAT 
CTGGTGAATG TCAAGTTGGT GGTGAATTTT GACATGCCAT CGAGTATGGA TGAGTATGTG 
CACCAGATTG GCAGAGCAGG AAGGCTTGGC CACAGGGGAA CAGCTATAAC TTTCATCAAC 
AAGAACAACC GCAACCTCTT CTGGGACCTG GTGAAGAGAG TGCAGCCCAC TGGCTCTTTG 
CTGCCCCCGC AGCTGCTAAA TTCCCCCTAT GTACAAGAAC AGAAAAAAGT TGACGAAAGA 
TTGCGGCGGG ACAAAGAGAA GGTGGTAACG GGAGACCAAA TTCTTGATCT TATTCGAAAA 
CATGACAGGC ATAAATCTCA TAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817954.1
CDS	17..2083
Translation	MPAVSPAAPSLLTTEASSLHTSASPITPAPTATPSLTPAPTATPSLTPAPTATPSLTPAPTATPSLTPAPTSTQFLKAASSSTTPTPSVPTSAPSISCPSIPASFSQPIKLPPLSKSSEDAHEEAVKSYSKCQRWPFPGDPVCVICGRYGEYICDQTDHDVCSLECKAMDILRVCGASSTPCAITDITLSPQPLLYSAIGPLDNSAITSTVSTELTSCQNSDADTSHVTAYNHPMPYIYKEHEFISQLSADQINHLRQQLSLSVHGNEMCKPIMEFDHCQFPPVLSSNIKAAGYEVPTPIQMQMIPVGLMGRDILASADTGSGKTAAFLLPAIIRCLEKDSPAALILTPTRELAVQIEGQAKELMRGIPHMRTALLVGGMPLPPQIHRLKQGVQVIIATPGRLLEIIKQDSVNLGDLKILIVDEADTMLKMGFQQQVLDILEVVPHDHQTILVSATIPAGIEAFTKQLLQDPVRITVGEKNQPCTNVRQIVLWVEEPSKKKKLFEILNDSKLFQPPVLVFVDCRLGADLLSDAVSKITGLECVAMHSDKSQVERMKILQGLLQGEYDVVVSTGVLGRGLDLVNVKLVVNFDMPSSMDEYVHQIGRAGRLGHRGTAITFINKNNRNLFWDLVKRVQPTGSLLPPQLLNSPYVQEQKKVDERLRRDKEKVVTGDQILDLIRKHDRHKSHK

Target ORF information:

RefSeq Version	XM_012962500.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DEAD-box helicase 59 (ddx59), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012962500.1

ORF Insert Sequence:

ATGCCAGCTG TTAGTCCTGC TGCCCCATCT CTGCTCACTA CTGAGGCTTC GTCTTTACAC 
ACTTCTGCTT CGCCCATCAC ACCTGCACCC ACCGCTACCC CTTCCCTTAC ACCTGCACCC 
ACCGCTACCC CTTCCCTTAC ACCTGCACCC ACCGCTACCC CTTCCCTTAC ACCTGCACCC 
ACCGCTACCC CTTCCCTTAC ACCTGCACCC ACCTCCACTC AATTCCTCAA GGCTGCAAGC 
TCTTCTACAA CACCCACACC ATCCGTACCC ACCTCTGCTC CATCCATATC ATGTCCTTCC 
ATCCCTGCTT CTTTCTCACA ACCTATCAAA CTCCCACCAT TATCCAAATC TTCAGAGGAT 
GCCCATGAAG AAGCAGTTAA GTCATACAGT AAATGTCAGC GCTGGCCGTT TCCTGGGGAT 
CCTGTATGTG TAATCTGTGG AAGATATGGA GAGTATATAT GTGACCAGAC GGACCATGAT 
GTGTGCAGCT TAGAGTGCAA AGCCATGGAC ATTTTGCGGG TTTGTGGAGC ATCCAGCACC 
CCATGTGCTA TCACAGACAT TACCTTAAGC CCACAGCCTT TATTGTATTC AGCTATTGGG 
CCTCTGGATA ATTCAGCCAT CACTTCTACA GTCTCCACTG AACTGACCTC ATGCCAAAAT 
AGTGATGCAG ATACTTCACA TGTGACCGCA TACAACCACC CAATGCCTTA TATCTACAAG 
GAACATGAGT TCATCTCACA GTTAAGCGCT GACCAGATAA ACCACCTGAG ACAGCAGCTT 
AGCTTGTCAG TACATGGGAA TGAGATGTGC AAACCTATCA TGGAGTTTGA CCACTGTCAG 
TTTCCTCCTG TGCTCAGCTC TAACATAAAG GCAGCAGGTT ATGAAGTGCC TACTCCCATA 
CAGATGCAGA TGATCCCAGT GGGGCTTATG GGAAGAGATA TATTGGCAAG TGCAGATACT 
GGCTCAGGGA AAACTGCAGC TTTTCTTCTT CCAGCTATAA TTCGATGTCT TGAAAAGGAT 
TCTCCAGCTG CACTGATTCT TACACCCACT AGAGAACTGG CTGTGCAGAT AGAGGGACAG 
GCCAAAGAAC TGATGCGTGG GATTCCTCAC ATGAGGACTG CACTACTTGT GGGCGGGATG 
CCTCTGCCCC CTCAGATACA CCGTCTGAAA CAAGGTGTAC AGGTTATAAT AGCAACTCCT 
GGGAGACTTC TAGAAATCAT TAAGCAGGAT TCTGTGAATC TTGGTGATCT AAAGATTCTT 
ATTGTGGATG AGGCAGATAC CATGCTGAAG ATGGGGTTTC AGCAGCAAGT CCTAGATATT 
TTGGAGGTTG TACCACACGA CCATCAGACC ATTCTGGTTT CAGCTACCAT CCCAGCTGGC 
ATTGAGGCCT TCACAAAGCA GCTCCTGCAG GATCCTGTGC GAATCACTGT AGGCGAGAAA 
AACCAGCCCT GCACCAACGT GAGGCAGATT GTGCTGTGGG TGGAGGAACC TTCAAAGAAG 
AAAAAGCTCT TTGAAATTTT GAATGATTCC AAGCTTTTCC AGCCCCCCGT CTTAGTGTTT 
GTGGATTGCC GTCTCGGTGC TGATCTGCTG AGTGATGCCG TCAGTAAAAT CACAGGGTTA 
GAGTGTGTGG CAATGCACTC TGATAAATCC CAGGTGGAAC GAATGAAGAT CTTGCAGGGT 
TTGCTCCAGG GAGAATATGA TGTTGTGGTG AGCACTGGAG TTCTGGGGCG TGGGCTGGAT 
CTGGTGAATG TCAAGTTGGT GGTGAATTTT GACATGCCAT CGAGTATGGA TGAGTATGTG 
CACCAGATTG GCAGAGCAGG AAGGCTTGGC CACAGGGGAA CAGCTATAAC TTTCATCAAC 
AAGAACAACC GCAACCTCTT CTGGGACCTG GTGAAGAGAG TGCAGCCCAC TGGCTCTTTG 
CTGCCCCCGC AGCTGCTAAA TTCCCCCTAT GTACAAGAAC AGAAAAAAGT TGACGAAAGA 
TTGCGGCGGG ACAAAGAGAA GGTGGTAACG GGAGACCAAA TTCTTGATCT TATTCGAAAA 
CATGACAGGC ATAAATCTCA TAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ddx59 ( XM_018093631.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949120.1 Xenopus tropicalis DEAD-box helicase 59 (ddx59), transcript variant X3, mRNA.

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CloneID	OXa44204
Clone ID Related Accession (Same CDS sequence)	XM_018093631.1
Accession Version	XM_018093631.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	probable ATP-dependent RNA helicase DDX59 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGCTG TTAGTCCTGC TGCCCCATCT CTGCTCACTA CTGAGGCTTC GTCTTTACAC 
ACTTCTGCTT CGCCCATCAC ACCTGCACCC ACCGCTACCC CTTCCCTTAC ACCTGCACCC 
ACCGCTACCC CTTCCCTTAC ACCTGCACCC ACCGCTACCC CTTCCCTTAC ACCTGCACCC 
ACCGCTACCC CTTCCCTTAC ACCTGCACCC ACCTCCACTC AATTCCTCAA GGCTGCAAGC 
TCTTCTACAA CACCCACACC ATCCGTACCC ACCTCTGCTC CATCCATATC ATGTCCTTCC 
ATCCCTGCTT CTTTCTCACA ACCTATCAAA CTCCCACCAT TATCCAAATC TTCAGAGGAT 
GCCCATGAAG AAGCAGTTAA GTCATACAGT AAATGTCAGC GCTGGCCGTT TCCTGGGGAT 
CCTGTATGTG TAATCTGTGG AAGATATGGA GAGTATATAT GTGACCAGAC GGACCATGAT 
GTGTGCAGCT TAGAGTGCAA AGCCATGGAC ATTTTGCGGG TTTGTGGAGC ATCCAGCACC 
CCATGTGCTA TCACAGACAT TACCTTAAGC CCACAGCCTT TATTGTATTC AGCTATTGGG 
CCTCTGGATA ATTCAGCCAT CACTTCTACA GTCTCCACTG AACTGACCTC ATGCCAAAAT 
AGTGATGCAG ATACTTCACA TGTGACCGCA TACAACCACC CAATGCCTTA TATCTACAAG 
GAACATGAGT TCATCTCACA GTTAAGCGCT GACCAGATAA ACCACCTGAG ACAGCAGCTT 
AGCTTGTCAG TACATGGGAA TGAGATGTGC AAACCTATCA TGGAGTTTGA CCACTGTCAG 
TTTCCTCCTG TGCTCAGCTC TAACATAAAG GCAGCAGGTT ATGAAGTGCC TACTCCCATA 
CAGATGCAGA TGATCCCAGT GGGGCTTATG GGAAGAGATA TATTGGCAAG TGCAGATACT 
GGCTCAGGGA AAACTGCAGC TTTTCTTCTT CCAGCTATAA TTCGATGTCT TGAAAAGAAG 
GATTCTCCAG CTGCACTGAT TCTTACACCC ACTAGAGAAC TGGCTGTGCA GATAGAGGGA 
CAGGCCAAAG AACTGATGCG TGGGATTCCT CACATGAGGA CTGCACTACT TGTGGGCGGG 
ATGCCTCTGC CCCCTCAGAT ACACCGTCTG AAACAAGGTG TACAGGTTAT AATAGCAACT 
CCTGGGAGAC TTCTAGAAAT CATTAAGCAG GATTCTGTGA ATCTTGGTGA TCTAAAGATT 
CTTATTGTGG ATGAGGCAGA TACCATGCTG AAGATGGGGT TTCAGCAGCA AGTCCTAGAT 
ATTTTGGAGG TTGTACCACA CGACCATCAG ACCATTCTGG TTTCAGCTAC CATCCCAGCT 
GGCATTGAGG CCTTCACAAA GCAGCTCCTG CAGGATCCTG TGCGAATCAC TGTAGGCGAG 
AAAAACCAGC CCTGCACCAA CGTGAGGCAG ATTGTGCTGT GGGTGGAGGA ACCTTCAAAG 
AAGAAAAAGC TCTTTGAAAT TTTGAATGAT TCCAAGCTTT TCCAGCCCCC CGTCTTAGTG 
TTTGTGGATT GCCGTCTCGG TGCTGATCTG CTGAGTGATG CCGTCAGTAA AATCACAGGG 
TTAGAGTGTG TGGCAATGCA CTCTGATAAA TCCCAGGTGG AACGAATGAA GATCTTGCAG 
ATTGGCAGAG CAGGAAGGCT TGGCCACAGG GGAACAGCTA TAACTTTCAT CAACAAGAAC 
AACCGCAACC TCTTCTGGGA CCTGGTGAAG AGAGTGCAGC CCACTGGCTC TTTGCTGCCC 
CCGCAGCTGC TAAATTCCCC CTATGTACAA GAACAGAAAA AAGTTGACGA AAGATTGCGG 
CGGGACAAAG AGAAGGTGGT AACGGGAGAC CAAATTCTTG ATCTTATTCG AAAACATGAC 
AGGCATAAAT CTCATAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949120.1
CDS	17..1957
Translation	MPAVSPAAPSLLTTEASSLHTSASPITPAPTATPSLTPAPTATPSLTPAPTATPSLTPAPTATPSLTPAPTSTQFLKAASSSTTPTPSVPTSAPSISCPSIPASFSQPIKLPPLSKSSEDAHEEAVKSYSKCQRWPFPGDPVCVICGRYGEYICDQTDHDVCSLECKAMDILRVCGASSTPCAITDITLSPQPLLYSAIGPLDNSAITSTVSTELTSCQNSDADTSHVTAYNHPMPYIYKEHEFISQLSADQINHLRQQLSLSVHGNEMCKPIMEFDHCQFPPVLSSNIKAAGYEVPTPIQMQMIPVGLMGRDILASADTGSGKTAAFLLPAIIRCLEKKDSPAALILTPTRELAVQIEGQAKELMRGIPHMRTALLVGGMPLPPQIHRLKQGVQVIIATPGRLLEIIKQDSVNLGDLKILIVDEADTMLKMGFQQQVLDILEVVPHDHQTILVSATIPAGIEAFTKQLLQDPVRITVGEKNQPCTNVRQIVLWVEEPSKKKKLFEILNDSKLFQPPVLVFVDCRLGADLLSDAVSKITGLECVAMHSDKSQVERMKILQIGRAGRLGHRGTAITFINKNNRNLFWDLVKRVQPTGSLLPPQLLNSPYVQEQKKVDERLRRDKEKVVTGDQILDLIRKHDRHKSHK

Target ORF information:

RefSeq Version	XM_018093631.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DEAD-box helicase 59 (ddx59), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018093631.1

ORF Insert Sequence:

ATGCCAGCTG TTAGTCCTGC TGCCCCATCT CTGCTCACTA CTGAGGCTTC GTCTTTACAC 
ACTTCTGCTT CGCCCATCAC ACCTGCACCC ACCGCTACCC CTTCCCTTAC ACCTGCACCC 
ACCGCTACCC CTTCCCTTAC ACCTGCACCC ACCGCTACCC CTTCCCTTAC ACCTGCACCC 
ACCGCTACCC CTTCCCTTAC ACCTGCACCC ACCTCCACTC AATTCCTCAA GGCTGCAAGC 
TCTTCTACAA CACCCACACC ATCCGTACCC ACCTCTGCTC CATCCATATC ATGTCCTTCC 
ATCCCTGCTT CTTTCTCACA ACCTATCAAA CTCCCACCAT TATCCAAATC TTCAGAGGAT 
GCCCATGAAG AAGCAGTTAA GTCATACAGT AAATGTCAGC GCTGGCCGTT TCCTGGGGAT 
CCTGTATGTG TAATCTGTGG AAGATATGGA GAGTATATAT GTGACCAGAC GGACCATGAT 
GTGTGCAGCT TAGAGTGCAA AGCCATGGAC ATTTTGCGGG TTTGTGGAGC ATCCAGCACC 
CCATGTGCTA TCACAGACAT TACCTTAAGC CCACAGCCTT TATTGTATTC AGCTATTGGG 
CCTCTGGATA ATTCAGCCAT CACTTCTACA GTCTCCACTG AACTGACCTC ATGCCAAAAT 
AGTGATGCAG ATACTTCACA TGTGACCGCA TACAACCACC CAATGCCTTA TATCTACAAG 
GAACATGAGT TCATCTCACA GTTAAGCGCT GACCAGATAA ACCACCTGAG ACAGCAGCTT 
AGCTTGTCAG TACATGGGAA TGAGATGTGC AAACCTATCA TGGAGTTTGA CCACTGTCAG 
TTTCCTCCTG TGCTCAGCTC TAACATAAAG GCAGCAGGTT ATGAAGTGCC TACTCCCATA 
CAGATGCAGA TGATCCCAGT GGGGCTTATG GGAAGAGATA TATTGGCAAG TGCAGATACT 
GGCTCAGGGA AAACTGCAGC TTTTCTTCTT CCAGCTATAA TTCGATGTCT TGAAAAGAAG 
GATTCTCCAG CTGCACTGAT TCTTACACCC ACTAGAGAAC TGGCTGTGCA GATAGAGGGA 
CAGGCCAAAG AACTGATGCG TGGGATTCCT CACATGAGGA CTGCACTACT TGTGGGCGGG 
ATGCCTCTGC CCCCTCAGAT ACACCGTCTG AAACAAGGTG TACAGGTTAT AATAGCAACT 
CCTGGGAGAC TTCTAGAAAT CATTAAGCAG GATTCTGTGA ATCTTGGTGA TCTAAAGATT 
CTTATTGTGG ATGAGGCAGA TACCATGCTG AAGATGGGGT TTCAGCAGCA AGTCCTAGAT 
ATTTTGGAGG TTGTACCACA CGACCATCAG ACCATTCTGG TTTCAGCTAC CATCCCAGCT 
GGCATTGAGG CCTTCACAAA GCAGCTCCTG CAGGATCCTG TGCGAATCAC TGTAGGCGAG 
AAAAACCAGC CCTGCACCAA CGTGAGGCAG ATTGTGCTGT GGGTGGAGGA ACCTTCAAAG 
AAGAAAAAGC TCTTTGAAAT TTTGAATGAT TCCAAGCTTT TCCAGCCCCC CGTCTTAGTG 
TTTGTGGATT GCCGTCTCGG TGCTGATCTG CTGAGTGATG CCGTCAGTAA AATCACAGGG 
TTAGAGTGTG TGGCAATGCA CTCTGATAAA TCCCAGGTGG AACGAATGAA GATCTTGCAG 
ATTGGCAGAG CAGGAAGGCT TGGCCACAGG GGAACAGCTA TAACTTTCAT CAACAAGAAC 
AACCGCAACC TCTTCTGGGA CCTGGTGAAG AGAGTGCAGC CCACTGGCTC TTTGCTGCCC 
CCGCAGCTGC TAAATTCCCC CTATGTACAA GAACAGAAAA AAGTTGACGA AAGATTGCGG 
CGGGACAAAG AGAAGGTGGT AACGGGAGAC CAAATTCTTG ATCTTATTCG AAAACATGAC 
AGGCATAAAT CTCATAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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ddx59 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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