lrrc8b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	lrrc8b
Entrez Gene ID	100488692
Full Name	leucine rich repeat containing 8 VRAC subunit B
General protein information	Preferred Names leucine rich repeat containing 8 VRAC subunit B Names volume-regulated anion channel subunit LRRC8B leucine-rich repeat containing 8 family member B
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002941126.4	XP_002941172.1	volume-regulated anion channel subunit LRRC8B
XM_002941126.5	XP_002941172.2	volume-regulated anion channel subunit LRRC8B

Pathways from BioSystems

Homologies

Homo sapiens (human)	LRRC8B	NP_056165.1
Rattus norvegicus (Norway rat)	Lrrc8b	NP_001100674.1
Gallus gallus (chicken)	LRRC8B	XP_004936694.1
	LRRC8B	XP_002801670.1
Pan troglodytes (chimpanzee)	LRRC8B	XP_001150409.1
Bos taurus (cattle)	LRRC8B	XP_005204398.1
Mus musculus (house mouse)	Lrrc8b	NP_001028722.1
Canis lupus familiaris (dog)	LRRC8B	XP_005622010.1
Xenopus tropicalis (tropical clawed frog)	lrrc8b	XP_002941172.1

Gene Ontology

The following lrrc8b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lrrc8b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa09074	XM_002941126.4 Latest version!	Xenopus tropicalis leucine-rich repeat containing 8 family member B (lrrc8b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa57278	XM_002941126.5 Latest version!	Xenopus tropicalis leucine rich repeat containing 8 VRAC subunit B (lrrc8b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lrrc8b ( XM_002941126.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002941172.1 Xenopus tropicalis leucine-rich repeat containing 8 family member B (lrrc8b), mRNA.

Price & Availability↑

CloneID	OXa09074
Clone ID Related Accession (Same CDS sequence)	XM_002941126.4
Accession Version	XM_002941126.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2394bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	volume-regulated anion channel subunit LRRC8B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002941126.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCACTG TGACCGAGCT CAGATTTCTG GCAGACGCTC AAACATCTTA TCACATTTTA 
AAGCCATGGT GGGATGTATT TTGGTATTAC ATTACTATGG TTATGTTAAT GATTGCAGTG 
TTAGCAGGCG CACTGCAACT CACCCAAAGC AGAATGCTGT GCCTTCCCTG TAAAGCTGAA 
GTGGACAATC AGTGTGTACA TCCTTTGGAA CCAGTGCTTA GACATATTAA CTTATCATCT 
GCTTCTGCAG TTCAGGCAAT TCCATCGTTA GGCATTCAGA ATGACCTTCA CCGCCAACAG 
TATGCTTACA TTGATGCAGT ATGTTATGAG AAACAGCTTC ACTGGTTCGC CAAATTCTTC 
CCCTACCTTG TGTTTCTGCA CTCAATAGTC TTTGCAGTTT GCAGCAACTT CTGGCTTCAC 
TATCCAAGTA CAAGTTCTAG ACTGGAGCAT TTTGTAGCAA TTCTTTATAA ATGTTTTGAT 
TCCCCATGGA CAACACGGGC TTTGTCAGAG GCAGTTGCTC AACAATCAAT AAAACCACAG 
CATCGGAGAA GATCCCAGCT TATGACTTCT GCTGGTACTG AAGACGCAGA GACTCGCAGG 
CAATCACTGC AGCCTGGAAT TGAGTCTTCA GCAGTAGATG ATGATGCCTC AGGTGTCCTA 
GACAAAAAAG AAGGTGAACA AGCCAAGGCT TTGTTTGAGA GAGTTCGTAG GTTCAGGTTC 
CATGTTGAAC AAAAGGATAT TATATACAGA TTGTATATGA AGCAAATTGT AGTTAAAGTA 
GTTGCACTCA TTGTGATAAT TGCATACGTG TCTTATTACT TGATGAATAT AACATTTGAA 
ATTGACTGCA TTATTGATAT TCAAGCATTT ACAGGGTACA GGAGATACCA ATGTGTTTAC 
TCTCTTGCAG TAATTTTCAA AGTGCTGGCT ACATTTTATG TTTTTCTTGT GCTTCTGTAC 
GGTTATGCCT GCTTTCATAG TCTGTGGTGG ATGCTTAGAA GTTCTCTCAA GCGATATTCA 
TTTGAAGCAG TGAGAGAAAA AAGCAATTAC AGTGACATTC CAGATGTACA AAATGACTTT 
GCCTTCATCC TTCACTTAGC AGATCAATAC GATCCCTTGT ACGCCAAGCG GTTTTCGATA 
TTTTTGTCAG AGGTGAGTGA GAACAAATTG AAACAAATTA ATCTCAACAA TGAATGGACA 
GTGGAAATGC TGAGAAATAA GTTACGGCGC AGTTCTAAAG ACAAAGTGGA GCTTCATATG 
TTCATGTTGA GTGGGTTGCC AGACAATGTC TTTGAACTTA ACGAGATTGA TGTACTGATC 
TTGGAACTTA TACCAGATGC CAAACTGACG CCTATGATAA CCCAACTTGT AAATCTTAAG 
GAACTTCATA TTTACCATTC CACACTTACT GTAGACTTGC AGGCACTTAG CTTCCTTGCA 
GAGAACCTGG AAACGCTGCA TTTAAAATTC ACTACAATGG AGAAGATTCC ATCCTGGCTT 
TTTCTTTTGA AAAATTTGAA GGAATTGTAC TTAGCAGGAT CACTTGACAA TTACAGTTAT 
TTTTACATTG AAGGCCTTCA GGACTTGAAG AACCTGAAAT CTCTTTATTT GAAGAGCAGC 
CTTCCCAGGA TCCCTCCTGT AGTCACAGAA TCATTGCCAT CACTGGAGAA ACTGTCTATT 
AATAATGAAG GGAACAGACT GATGGTCTTA ATCAGTTTGA AAAAAATGAC CAACCTCGCT 
TGCCTGGAAC TGATCAACTG TGACTTAGAA CGAATTCCAC ACTCCATTTT CAGTCTCCGA 
GAACTGCAGG AGATTGATTT TAGAGGAAAT AATTTTAAGA CGATTGAGGA AATAATTAGC 
TTTCAGCATC TGCAGAAATT GACCTGTTTG AAGCTGTGGC ACAATTCTAT TGCTTACATT 
CCAATTCAGA TCGGAGTTCT AGCAAACCTA GAACAGCTCT ACCTAAATCA CAATAACATT 
GAAATGGTGC CTCTTCAACT GTTCTTATGT AACAAGCTCC GGGTTCTGGA CTTAAGCTAT 
AACAATTTAT CCTTTATCCC AGAGGAAATA CAGCTATTAA AGAACCTTCA GTATTTTGCT 
GTGACCAAAA ACAGTATTGA AACGCTGCCT GATGGACTAT TTAAATGCAG CAAACTTCAG 
TATTTATTAC TTGGAAAAAA CAGCTTGATG AGCATCTCCC CACAAGTTGG CGACCTTATG 
AACCTCATTA AGATTGAGCT TATTGGTAAC CAAGTTGAAT CTCTTCCTCC TGAAATAGAA 
TCTTGCCAGT CTCTAAAACC AAATTGCATT ATTGTGGAAA CACATGTGTT ACGTACCCTT 
CCATCACTTG CAAAGGAAAA TCTCAAACCG TTACAGAGAG AGAAGATGTC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002941172.1
CDS	283..2676
Translation	MITVTELRFLADAQTSYHILKPWWDVFWYYITMVMLMIAVLAGALQLTQSRMLCLPCKAEVDNQCVHPLEPVLRHINLSSASAVQAIPSLGIQNDLHRQQYAYIDAVCYEKQLHWFAKFFPYLVFLHSIVFAVCSNFWLHYPSTSSRLEHFVAILYKCFDSPWTTRALSEAVAQQSIKPQHRRRSQLMTSAGTEDAETRRQSLQPGIESSAVDDDASGVLDKKEGEQAKALFERVRRFRFHVEQKDIIYRLYMKQIVVKVVALIVIIAYVSYYLMNITFEIDCIIDIQAFTGYRRYQCVYSLAVIFKVLATFYVFLVLLYGYACFHSLWWMLRSSLKRYSFEAVREKSNYSDIPDVQNDFAFILHLADQYDPLYAKRFSIFLSEVSENKLKQINLNNEWTVEMLRNKLRRSSKDKVELHMFMLSGLPDNVFELNEIDVLILELIPDAKLTPMITQLVNLKELHIYHSTLTVDLQALSFLAENLETLHLKFTTMEKIPSWLFLLKNLKELYLAGSLDNYSYFYIEGLQDLKNLKSLYLKSSLPRIPPVVTESLPSLEKLSINNEGNRLMVLISLKKMTNLACLELINCDLERIPHSIFSLRELQEIDFRGNNFKTIEEIISFQHLQKLTCLKLWHNSIAYIPIQIGVLANLEQLYLNHNNIEMVPLQLFLCNKLRVLDLSYNNLSFIPEEIQLLKNLQYFAVTKNSIETLPDGLFKCSKLQYLLLGKNSLMSISPQVGDLMNLIKIELIGNQVESLPPEIESCQSLKPNCIIVETHVLRTLPSLAKENLKPLQREKMS

Target ORF information:

RefSeq Version	XM_002941126.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis leucine-rich repeat containing 8 family member B (lrrc8b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002941126.4

ORF Insert Sequence:

ATGATCACTG TGACCGAGCT CAGATTTCTG GCAGACGCTC AAACATCTTA TCACATTTTA 
AAGCCATGGT GGGATGTATT TTGGTATTAC ATTACTATGG TTATGTTAAT GATTGCAGTG 
TTAGCAGGCG CACTGCAACT CACCCAAAGC AGAATGCTGT GCCTTCCCTG TAAAGCTGAA 
GTGGACAATC AGTGTGTACA TCCTTTGGAA CCAGTGCTTA GACATATTAA CTTATCATCT 
GCTTCTGCAG TTCAGGCAAT TCCATCGTTA GGCATTCAGA ATGACCTTCA CCGCCAACAG 
TATGCTTACA TTGATGCAGT ATGTTATGAG AAACAGCTTC ACTGGTTCGC CAAATTCTTC 
CCCTACCTTG TGTTTCTGCA CTCAATAGTC TTTGCAGTTT GCAGCAACTT CTGGCTTCAC 
TATCCAAGTA CAAGTTCTAG ACTGGAGCAT TTTGTAGCAA TTCTTTATAA ATGTTTTGAT 
TCCCCATGGA CAACACGGGC TTTGTCAGAG GCAGTTGCTC AACAATCAAT AAAACCACAG 
CATCGGAGAA GATCCCAGCT TATGACTTCT GCTGGTACTG AAGACGCAGA GACTCGCAGG 
CAATCACTGC AGCCTGGAAT TGAGTCTTCA GCAGTAGATG ATGATGCCTC AGGTGTCCTA 
GACAAAAAAG AAGGTGAACA AGCCAAGGCT TTGTTTGAGA GAGTTCGTAG GTTCAGGTTC 
CATGTTGAAC AAAAGGATAT TATATACAGA TTGTATATGA AGCAAATTGT AGTTAAAGTA 
GTTGCACTCA TTGTGATAAT TGCATACGTG TCTTATTACT TGATGAATAT AACATTTGAA 
ATTGACTGCA TTATTGATAT TCAAGCATTT ACAGGGTACA GGAGATACCA ATGTGTTTAC 
TCTCTTGCAG TAATTTTCAA AGTGCTGGCT ACATTTTATG TTTTTCTTGT GCTTCTGTAC 
GGTTATGCCT GCTTTCATAG TCTGTGGTGG ATGCTTAGAA GTTCTCTCAA GCGATATTCA 
TTTGAAGCAG TGAGAGAAAA AAGCAATTAC AGTGACATTC CAGATGTACA AAATGACTTT 
GCCTTCATCC TTCACTTAGC AGATCAATAC GATCCCTTGT ACGCCAAGCG GTTTTCGATA 
TTTTTGTCAG AGGTGAGTGA GAACAAATTG AAACAAATTA ATCTCAACAA TGAATGGACA 
GTGGAAATGC TGAGAAATAA GTTACGGCGC AGTTCTAAAG ACAAAGTGGA GCTTCATATG 
TTCATGTTGA GTGGGTTGCC AGACAATGTC TTTGAACTTA ACGAGATTGA TGTACTGATC 
TTGGAACTTA TACCAGATGC CAAACTGACG CCTATGATAA CCCAACTTGT AAATCTTAAG 
GAACTTCATA TTTACCATTC CACACTTACT GTAGACTTGC AGGCACTTAG CTTCCTTGCA 
GAGAACCTGG AAACGCTGCA TTTAAAATTC ACTACAATGG AGAAGATTCC ATCCTGGCTT 
TTTCTTTTGA AAAATTTGAA GGAATTGTAC TTAGCAGGAT CACTTGACAA TTACAGTTAT 
TTTTACATTG AAGGCCTTCA GGACTTGAAG AACCTGAAAT CTCTTTATTT GAAGAGCAGC 
CTTCCCAGGA TCCCTCCTGT AGTCACAGAA TCATTGCCAT CACTGGAGAA ACTGTCTATT 
AATAATGAAG GGAACAGACT GATGGTCTTA ATCAGTTTGA AAAAAATGAC CAACCTCGCT 
TGCCTGGAAC TGATCAACTG TGACTTAGAA CGAATTCCAC ACTCCATTTT CAGTCTCCGA 
GAACTGCAGG AGATTGATTT TAGAGGAAAT AATTTTAAGA CGATTGAGGA AATAATTAGC 
TTTCAGCATC TGCAGAAATT GACCTGTTTG AAGCTGTGGC ACAATTCTAT TGCTTACATT 
CCAATTCAGA TCGGAGTTCT AGCAAACCTA GAACAGCTCT ACCTAAATCA CAATAACATT 
GAAATGGTGC CTCTTCAACT GTTCTTATGT AACAAGCTCC GGGTTCTGGA CTTAAGCTAT 
AACAATTTAT CCTTTATCCC AGAGGAAATA CAGCTATTAA AGAACCTTCA GTATTTTGCT 
GTGACCAAAA ACAGTATTGA AACGCTGCCT GATGGACTAT TTAAATGCAG CAAACTTCAG 
TATTTATTAC TTGGAAAAAA CAGCTTGATG AGCATCTCCC CACAAGTTGG CGACCTTATG 
AACCTCATTA AGATTGAGCT TATTGGTAAC CAAGTTGAAT CTCTTCCTCC TGAAATAGAA 
TCTTGCCAGT CTCTAAAACC AAATTGCATT ATTGTGGAAA CACATGTGTT ACGTACCCTT 
CCATCACTTG CAAAGGAAAA TCTCAAACCG TTACAGAGAG AGAAGATGTC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lrrc8b ( XM_002941126.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002941172.2 Xenopus tropicalis leucine rich repeat containing 8 VRAC subunit B (lrrc8b), mRNA.

Price & Availability↑

CloneID	OXa57278
Clone ID Related Accession (Same CDS sequence)	XM_002941126.5
Accession Version	XM_002941126.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2394bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	volume-regulated anion channel subunit LRRC8B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002941126.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCACTG TGACCGAGCT CAGATTTCTG GCAGACGCTC AAACATCTTA TCACATTTTA 
AAGCCATGGT GGGATGTATT TTGGTATTAC ATTACTATGG TTATGTTAAT GATTGCAGTG 
TTAGCAGGCG CACTGCAACT CACCCAAAGC AGAATGCTGT GCCTTCCCTG TAAAGCTGAA 
GTGGACAATC AGTGTGTACA TCCTTTGGAA CCAGTGCTTA GACATATTAA CTTATCATCT 
GCTTCTGCAG TTCAGGCAAT TCCATCGTTA GGCATTCAGA ATGACCTTCA CCGCCAACAG 
TATGCTTACA TTGATGCAGT ATGTTATGAG AAACAGCTTC ACTGGTTCGC CAAATTCTTC 
CCCTACCTTG TGTTTCTGCA CTCAATAGTC TTTGCAGTTT GCAGCAACTT CTGGCTTCAC 
TATCCAAGTA CAAGTTCTAG ACTGGAGCAT TTTGTAGCAA TTCTTTATAA ATGTTTTGAT 
TCCCCATGGA CAACACGGGC TTTGTCAGAG GCAGTTGCTC AACAATCAAT AAAACCACAG 
CATCGGAGAA GATCCCAGCT TATGACTTCT ACTGGTACTG AAGACGCAGA GACTCGCAGG 
CAATCACTGC AGCCTGGAAT TGAGTCTTCA GCAGTAGATG ATGATGCCTC AGGTGTCCTA 
GACAAAAAAG AAGGTGAACA AGCCAAGGCT TTGTTTGAGA GAGTTCGTAG GTTCAGGTTC 
CATGTTGAAC AAAAGGATAT TATATACAGA TTGTATATGA AGCAAATTGT AGTTAAAGTA 
GTTGCACTCA TTGTGATAAT TGCATACGTG TCTTATTACT TGATGAATAT AACATTTGAA 
ATTGACTGCA TTATTGATAT TCAAGCATTT ACAGGGTACA GGAGATACCA ATGTGTTTAC 
TCTCTTGCAG TAATTTTCAA AGTGCTGGCT ACATTTTATG TTTTTCTTGT GCTTCTGTAC 
GGTTATGCCT GCTTTCATAG TCTGTGGTGG ATGCTTAGAA GTTCTCTCAA GCGATATTCA 
TTTGAAGCAG TGAGAGAAAA AAGCAATTAC AGTGACATTC CAGATGTACA AAATGACTTT 
GCCTTCATCC TTCACTTAGC AGATCAATAC GATCCCTTGT ACGCCAAGCG GTTTTCGATA 
TTTTTGTCAG AGGTGAGTGA GAACAAATTG AAACAAATTA ATCTCAACAA TGAATGGACA 
GTGGAAATGC TGAGAAATAA GTTACGGCGC AGTTCTAAAG ACAAAGTGGA GCTTCATATG 
TTCATGTTGA GTGGGTTGCC AGACAATGTC TTTGAACTTA ACGAGATTGA TGTACTGATC 
TTGGAACTTA TACCAGATGC CAAACTGACG CCTATGATAA CCCAACTTGT AAATCTTAAG 
GAACTTCATA TTTACCATTC CACACTTACT GTAGACTTGC AGGCACTTAG CTTCCTTGCA 
GAGAACCTGG AAACGCTGCA TTTAAAATTC ACTACAATGG AGAAGATTCC ATCCTGGCTT 
TTTCTTTTGA AAAATTTGAA GGAATTGTAC TTAGCAGGAT CACTTGACAA TTACAGTTAT 
TTTTACATTG AAGGCCTTCA GGACTTGAAG AACCTGAAAT CTCTTTATTT GAAGAGCAGC 
CTTCCCAGGA TCCCTCCTGT AGTCACAGAA TCATTGCCAT CACTGGAGAA ACTGTCTATT 
AATAATGAAG GGAACAGACT GATGGTCTTA ATCAGTTTGA AAAAAATGAC CAACCTCGCT 
TGCCTGGAAC TGATCAACTG TGACTTAGAA CGAATTCCAC ACTCCATTTT CAGTCTCCGA 
GAACTGCAGG AGATTGATTT TAGAGGAAAT AATTTTAAGA CGATTGAGGA AATAATTAGC 
TTTCAGCATC TGCAGAAATT GACCTGTTTG AAGCTGTGGC ACAATTCTAT TGCTTACATT 
CCAATTCAGA TCGGAGTTCT AGCAAACCTA GAACAGCTCT ACCTAAATCA CAATAACATT 
GAAATGGTGC CTCTTCAACT GTTCTTATGT AACAAGCTCC GGGTTCTGGA CTTAAGCTAT 
AACAATTTAT CCTTTATCCC AGAGGAAATA CAGCTATTAA AGAACCTTCA GTATTTTGCT 
GTGACCAAAA ACAGTATTGA AACGCTGCCT GATGGACTAT TTAAATGCAG CAAACTTCAG 
TATTTATTAC TTGGAAAAAA CAGCTTGATG AGCATCTCCC CACAAGTTGG CGACCTTATG 
AACCTCATTA AGATTGAGCT TATTGGTAAC CAAGTTGAAT CTCTTCCTCC TGAAATAGAA 
TCTTGCCAGT CTCTAAAACC AAATTGCATT ATTGTGGAAA CACATGTGTT ACGTACCCTT 
CCATCACTTG CAAAGGAAAA TCTCAAACCG TTACAGAGAG AGAAGATGTC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002941172.2
CDS	393..2786
Translation	MITVTELRFLADAQTSYHILKPWWDVFWYYITMVMLMIAVLAGALQLTQSRMLCLPCKAEVDNQCVHPLEPVLRHINLSSASAVQAIPSLGIQNDLHRQQYAYIDAVCYEKQLHWFAKFFPYLVFLHSIVFAVCSNFWLHYPSTSSRLEHFVAILYKCFDSPWTTRALSEAVAQQSIKPQHRRRSQLMTSTGTEDAETRRQSLQPGIESSAVDDDASGVLDKKEGEQAKALFERVRRFRFHVEQKDIIYRLYMKQIVVKVVALIVIIAYVSYYLMNITFEIDCIIDIQAFTGYRRYQCVYSLAVIFKVLATFYVFLVLLYGYACFHSLWWMLRSSLKRYSFEAVREKSNYSDIPDVQNDFAFILHLADQYDPLYAKRFSIFLSEVSENKLKQINLNNEWTVEMLRNKLRRSSKDKVELHMFMLSGLPDNVFELNEIDVLILELIPDAKLTPMITQLVNLKELHIYHSTLTVDLQALSFLAENLETLHLKFTTMEKIPSWLFLLKNLKELYLAGSLDNYSYFYIEGLQDLKNLKSLYLKSSLPRIPPVVTESLPSLEKLSINNEGNRLMVLISLKKMTNLACLELINCDLERIPHSIFSLRELQEIDFRGNNFKTIEEIISFQHLQKLTCLKLWHNSIAYIPIQIGVLANLEQLYLNHNNIEMVPLQLFLCNKLRVLDLSYNNLSFIPEEIQLLKNLQYFAVTKNSIETLPDGLFKCSKLQYLLLGKNSLMSISPQVGDLMNLIKIELIGNQVESLPPEIESCQSLKPNCIIVETHVLRTLPSLAKENLKPLQREKMS

Target ORF information:

RefSeq Version	XM_002941126.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis leucine rich repeat containing 8 VRAC subunit B (lrrc8b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002941126.5

ORF Insert Sequence:

ATGATCACTG TGACCGAGCT CAGATTTCTG GCAGACGCTC AAACATCTTA TCACATTTTA 
AAGCCATGGT GGGATGTATT TTGGTATTAC ATTACTATGG TTATGTTAAT GATTGCAGTG 
TTAGCAGGCG CACTGCAACT CACCCAAAGC AGAATGCTGT GCCTTCCCTG TAAAGCTGAA 
GTGGACAATC AGTGTGTACA TCCTTTGGAA CCAGTGCTTA GACATATTAA CTTATCATCT 
GCTTCTGCAG TTCAGGCAAT TCCATCGTTA GGCATTCAGA ATGACCTTCA CCGCCAACAG 
TATGCTTACA TTGATGCAGT ATGTTATGAG AAACAGCTTC ACTGGTTCGC CAAATTCTTC 
CCCTACCTTG TGTTTCTGCA CTCAATAGTC TTTGCAGTTT GCAGCAACTT CTGGCTTCAC 
TATCCAAGTA CAAGTTCTAG ACTGGAGCAT TTTGTAGCAA TTCTTTATAA ATGTTTTGAT 
TCCCCATGGA CAACACGGGC TTTGTCAGAG GCAGTTGCTC AACAATCAAT AAAACCACAG 
CATCGGAGAA GATCCCAGCT TATGACTTCT ACTGGTACTG AAGACGCAGA GACTCGCAGG 
CAATCACTGC AGCCTGGAAT TGAGTCTTCA GCAGTAGATG ATGATGCCTC AGGTGTCCTA 
GACAAAAAAG AAGGTGAACA AGCCAAGGCT TTGTTTGAGA GAGTTCGTAG GTTCAGGTTC 
CATGTTGAAC AAAAGGATAT TATATACAGA TTGTATATGA AGCAAATTGT AGTTAAAGTA 
GTTGCACTCA TTGTGATAAT TGCATACGTG TCTTATTACT TGATGAATAT AACATTTGAA 
ATTGACTGCA TTATTGATAT TCAAGCATTT ACAGGGTACA GGAGATACCA ATGTGTTTAC 
TCTCTTGCAG TAATTTTCAA AGTGCTGGCT ACATTTTATG TTTTTCTTGT GCTTCTGTAC 
GGTTATGCCT GCTTTCATAG TCTGTGGTGG ATGCTTAGAA GTTCTCTCAA GCGATATTCA 
TTTGAAGCAG TGAGAGAAAA AAGCAATTAC AGTGACATTC CAGATGTACA AAATGACTTT 
GCCTTCATCC TTCACTTAGC AGATCAATAC GATCCCTTGT ACGCCAAGCG GTTTTCGATA 
TTTTTGTCAG AGGTGAGTGA GAACAAATTG AAACAAATTA ATCTCAACAA TGAATGGACA 
GTGGAAATGC TGAGAAATAA GTTACGGCGC AGTTCTAAAG ACAAAGTGGA GCTTCATATG 
TTCATGTTGA GTGGGTTGCC AGACAATGTC TTTGAACTTA ACGAGATTGA TGTACTGATC 
TTGGAACTTA TACCAGATGC CAAACTGACG CCTATGATAA CCCAACTTGT AAATCTTAAG 
GAACTTCATA TTTACCATTC CACACTTACT GTAGACTTGC AGGCACTTAG CTTCCTTGCA 
GAGAACCTGG AAACGCTGCA TTTAAAATTC ACTACAATGG AGAAGATTCC ATCCTGGCTT 
TTTCTTTTGA AAAATTTGAA GGAATTGTAC TTAGCAGGAT CACTTGACAA TTACAGTTAT 
TTTTACATTG AAGGCCTTCA GGACTTGAAG AACCTGAAAT CTCTTTATTT GAAGAGCAGC 
CTTCCCAGGA TCCCTCCTGT AGTCACAGAA TCATTGCCAT CACTGGAGAA ACTGTCTATT 
AATAATGAAG GGAACAGACT GATGGTCTTA ATCAGTTTGA AAAAAATGAC CAACCTCGCT 
TGCCTGGAAC TGATCAACTG TGACTTAGAA CGAATTCCAC ACTCCATTTT CAGTCTCCGA 
GAACTGCAGG AGATTGATTT TAGAGGAAAT AATTTTAAGA CGATTGAGGA AATAATTAGC 
TTTCAGCATC TGCAGAAATT GACCTGTTTG AAGCTGTGGC ACAATTCTAT TGCTTACATT 
CCAATTCAGA TCGGAGTTCT AGCAAACCTA GAACAGCTCT ACCTAAATCA CAATAACATT 
GAAATGGTGC CTCTTCAACT GTTCTTATGT AACAAGCTCC GGGTTCTGGA CTTAAGCTAT 
AACAATTTAT CCTTTATCCC AGAGGAAATA CAGCTATTAA AGAACCTTCA GTATTTTGCT 
GTGACCAAAA ACAGTATTGA AACGCTGCCT GATGGACTAT TTAAATGCAG CAAACTTCAG 
TATTTATTAC TTGGAAAAAA CAGCTTGATG AGCATCTCCC CACAAGTTGG CGACCTTATG 
AACCTCATTA AGATTGAGCT TATTGGTAAC CAAGTTGAAT CTCTTCCTCC TGAAATAGAA 
TCTTGCCAGT CTCTAAAACC AAATTGCATT ATTGTGGAAA CACATGTGTT ACGTACCCTT 
CCATCACTTG CAAAGGAAAA TCTCAAACCG TTACAGAGAG AGAAGATGTC ATGA