frem1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following frem1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the frem1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa48725 XM_031890525.1
Latest version!
Xenopus tropicalis FRAS1 related extracellular matrix 1 (frem1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa48724 XM_031890523.1
Latest version!
Xenopus tropicalis FRAS1 related extracellular matrix 1 (frem1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa04017 XM_002939090.5
Latest version!
Xenopus tropicalis FRAS1 related extracellular matrix 1 (frem1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa42096 XM_018097904.1
Latest version!
Xenopus tropicalis FRAS1 related extracellular matrix 1 (frem1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OXa48723 XM_031890521.1
Latest version!
Xenopus tropicalis FRAS1 related extracellular matrix 1 (frem1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
View Old Accession Versions >>
OXa04017 XM_002939090.4 Xenopus tropicalis FRAS1 related extracellular matrix 1 (frem1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa48725
    Clone ID Related Accession (Same CDS sequence) XM_031890525.1
    Accession Version XM_031890525.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2550bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product FRAS1-related extracellular matrix protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAAGCCTT CTATTGTATA CCCAGTTTTG CTGTCAGTGT TGCTCACAAG GGGCCTCTGC 
    GCCTCCTTTA TCAAAGTCAA TCACGGAATG AAAGTTATGA AAGGACAGAG TGCATTCCTC
    TCAGAAAGTG ACCTGCAGTT TTCCATCCCA AGGGAAAAGG ATGCCTGCAA AGTGGAAGTA
    GTTGTTAATG AACCCATTAC ACAGAGAGTT GGAAGGTTAA CACCACAGGT GTTTGACTGC
    CATTTCCTAG CCAATGAAGT TCAGTACACA CACAATGGCT GCCCTATCTT GGATGAAGAC
    GAGGTCATGC TCAGACTTTA CAGATTTACA GAAACTGAGA CTTTTACTGA AACCTTCCTG
    CTGAGAGTGA AGTTAATAGA GCCTGACTGT AATATCATAC AGCTGGGGCC TAAAAAGCTT
    GAAGTGCCAG AGTTTTATGG TCTGTCTGAC GTTATTGATA AGAACATACT GAGCTTTGAC
    TATGACAAAA GAATAAACAT TGAATGTACC ATCAGAATAT CGTCTTCTGA ATCTTTCCTT
    CCTGCACACG GTCAGCTTGT GACGGGGGAT CCTGTGAAAC AAGAACCACG AGGAGATCAG
    CCACACAGCT TCTTCCATGG ATCAAATCAA AAGCCAGGAC CTTTGTGCAA GAATGGAGGC
    TGCATACAAG GTTTAAAGAG GATCCAGAGC GTTATAAAAA TGAGTTGTGA AGATTTCCTT
    GTAAAGGGCA TCCGATACCA GCACTTGGAT CCTCCATCTC CAAATGTTGA TTATATCAGT
    ATCAGGTTGG ATCTTACAGA CTCAAGGAGC AAATCACTTT ACAAGTCAGA ACATGCTTGG
    ATTCCTGTTC TCATTCGGGA TGCTGTTCCA AATCAAATCC CCAAAGCCAA CTTCATGTCT
    ATGTTTATCT TAGAAGTAGA TCAGTTTGTT CTAACACCCA TCTCAACTGC AACACTGGAT
    GCCGAGGACA GCGAAACAGC CAAACATCTT CTGGTCTTCA ATATTACTTT ACCTCCAAGC
    AAAGGGTTCA TCACACACTT GTCTGACCAT ACCAAGCCCA TTACATCCTT TACATGGAAA
    GATCTCAATG AAATGCTCAT AGCATATCAG CCTCCGAACA GCAGCCACAC AGAGAGAAGA
    AATTTGGAGG TGGAGTTTGA GGTGCATGAT TTCTTTTTTG AAAAGAGTCC TCCAATTACA
    GTGCATATCT CTATACGTAC GGCTGACACC AATGCTCCTC GCGTTTCATG GAACATGGGT
    CTGGATTTAC TGGAGGGCCA GTCTCGACCA ATTACATGGA AACAGTTCCA AATTGTGGAT
    AATGACAACA TCAATGCAGT TCGTTTGGTC ATTGTAGATG GATTGCAGCA TGGACGTATT
    ACTCTGAGAG GTGGGAAAGG ATTTATATTC ACAGTTAAGG ACATCCAGGC TGGAGATGTT
    TGCTACCATC ATGATGACAG TGACACTACT AAGGATTTTA TAGTCTTCAG AATATTTGAT
    GGACGTCACA GCATACGACA CAAGTTTCCT ATTAACATCC TACCAAAAGA TGATAGCCCT
    CCCTTCCTCA TCACAAACAT TGTTATTGAG CTTAGTGAAG GTCAAGCTGT CCTTATCGAG
    GGATCTATGC TTTGTGCTTC TGATATGGAC TCTAGTGATG CATACATACG TTTTAATATC
    ACTAAGCCTT CTGTAGCTGG AGAGATTATA AAGAAGCCAG GACAAAATCT TATTGCTTAT
    CCTGTCACAA GCTTCCTGCA ACGAGACTTA TATAACGGTT TCATTTATTA TCGCCATCTT
    GGAGGAGAAG TATTTGAAGA TTCATTTGAT GTTGTTCTGT CTGATAGCCA TGATCCACCA
    AACTTTTCTG GTCCTCAGGC AGTAATCATT CATATTAGCC CCGTGGATGA CCAGCTGCCC
    ATAGAAGCAC CTGGAACAGT ACGACATCTG ATGGTAAAGG AGAATGAGGT TGTTTATATA
    ACAAAGAAAC AACTGCATTT CATAGACCCA GAGTCACCTG AAAGAGAACT TATTTACACA
    GTTGCTACTC CTCCTTGTAT GGTGCCAACC TACAGCAGCT TAGATGCGGG GAAGATAGTT
    CTTGTAGACA CTATACTTAA ACTTGGAAAG GATTATGCAG GAGACACAGC AAGAACATTT
    TCACAGCATG CTGTGAATCA CCTCAAAGTT GCTTACATAC CACCAGCTAA AGACATTGGG
    CCAACTCCTC TACATGTCCA GTTTATATTA TCAGTGAGTA ACCAACATGG AGGAACAGTT
    CAAGGCATCT GTTTCAATAT CACTATCCTT CCAATAGACA ATCAGCCTCC TGAGGTGTTT
    TCATATGATT TGAAAGTTGA AGAAGGTGGT TTCAGCCAAA TTACTGCAAG TAACATTCAC
    ATCACAGATA AGGACACCAG ACAAGAACAA ATTAAAGTTA TTTTACACAA AGCTCCACTG
    CATGGTATCG TTCAACTTGA TGGATCTGCA TTGCAGGAAG GTGACCCATT CACCTGCTTG
    GACCTCATCA CATTAAAAGT ACCTTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031746385.1
    CDS620..3169
    Translation

    Target ORF information:

    RefSeq Version XM_031890525.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis FRAS1 related extracellular matrix 1 (frem1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031890525.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAAGCCTT CTATTGTATA CCCAGTTTTG CTGTCAGTGT TGCTCACAAG GGGCCTCTGC 
    GCCTCCTTTA TCAAAGTCAA TCACGGAATG AAAGTTATGA AAGGACAGAG TGCATTCCTC
    TCAGAAAGTG ACCTGCAGTT TTCCATCCCA AGGGAAAAGG ATGCCTGCAA AGTGGAAGTA
    GTTGTTAATG AACCCATTAC ACAGAGAGTT GGAAGGTTAA CACCACAGGT GTTTGACTGC
    CATTTCCTAG CCAATGAAGT TCAGTACACA CACAATGGCT GCCCTATCTT GGATGAAGAC
    GAGGTCATGC TCAGACTTTA CAGATTTACA GAAACTGAGA CTTTTACTGA AACCTTCCTG
    CTGAGAGTGA AGTTAATAGA GCCTGACTGT AATATCATAC AGCTGGGGCC TAAAAAGCTT
    GAAGTGCCAG AGTTTTATGG TCTGTCTGAC GTTATTGATA AGAACATACT GAGCTTTGAC
    TATGACAAAA GAATAAACAT TGAATGTACC ATCAGAATAT CGTCTTCTGA ATCTTTCCTT
    CCTGCACACG GTCAGCTTGT GACGGGGGAT CCTGTGAAAC AAGAACCACG AGGAGATCAG
    CCACACAGCT TCTTCCATGG ATCAAATCAA AAGCCAGGAC CTTTGTGCAA GAATGGAGGC
    TGCATACAAG GTTTAAAGAG GATCCAGAGC GTTATAAAAA TGAGTTGTGA AGATTTCCTT
    GTAAAGGGCA TCCGATACCA GCACTTGGAT CCTCCATCTC CAAATGTTGA TTATATCAGT
    ATCAGGTTGG ATCTTACAGA CTCAAGGAGC AAATCACTTT ACAAGTCAGA ACATGCTTGG
    ATTCCTGTTC TCATTCGGGA TGCTGTTCCA AATCAAATCC CCAAAGCCAA CTTCATGTCT
    ATGTTTATCT TAGAAGTAGA TCAGTTTGTT CTAACACCCA TCTCAACTGC AACACTGGAT
    GCCGAGGACA GCGAAACAGC CAAACATCTT CTGGTCTTCA ATATTACTTT ACCTCCAAGC
    AAAGGGTTCA TCACACACTT GTCTGACCAT ACCAAGCCCA TTACATCCTT TACATGGAAA
    GATCTCAATG AAATGCTCAT AGCATATCAG CCTCCGAACA GCAGCCACAC AGAGAGAAGA
    AATTTGGAGG TGGAGTTTGA GGTGCATGAT TTCTTTTTTG AAAAGAGTCC TCCAATTACA
    GTGCATATCT CTATACGTAC GGCTGACACC AATGCTCCTC GCGTTTCATG GAACATGGGT
    CTGGATTTAC TGGAGGGCCA GTCTCGACCA ATTACATGGA AACAGTTCCA AATTGTGGAT
    AATGACAACA TCAATGCAGT TCGTTTGGTC ATTGTAGATG GATTGCAGCA TGGACGTATT
    ACTCTGAGAG GTGGGAAAGG ATTTATATTC ACAGTTAAGG ACATCCAGGC TGGAGATGTT
    TGCTACCATC ATGATGACAG TGACACTACT AAGGATTTTA TAGTCTTCAG AATATTTGAT
    GGACGTCACA GCATACGACA CAAGTTTCCT ATTAACATCC TACCAAAAGA TGATAGCCCT
    CCCTTCCTCA TCACAAACAT TGTTATTGAG CTTAGTGAAG GTCAAGCTGT CCTTATCGAG
    GGATCTATGC TTTGTGCTTC TGATATGGAC TCTAGTGATG CATACATACG TTTTAATATC
    ACTAAGCCTT CTGTAGCTGG AGAGATTATA AAGAAGCCAG GACAAAATCT TATTGCTTAT
    CCTGTCACAA GCTTCCTGCA ACGAGACTTA TATAACGGTT TCATTTATTA TCGCCATCTT
    GGAGGAGAAG TATTTGAAGA TTCATTTGAT GTTGTTCTGT CTGATAGCCA TGATCCACCA
    AACTTTTCTG GTCCTCAGGC AGTAATCATT CATATTAGCC CCGTGGATGA CCAGCTGCCC
    ATAGAAGCAC CTGGAACAGT ACGACATCTG ATGGTAAAGG AGAATGAGGT TGTTTATATA
    ACAAAGAAAC AACTGCATTT CATAGACCCA GAGTCACCTG AAAGAGAACT TATTTACACA
    GTTGCTACTC CTCCTTGTAT GGTGCCAACC TACAGCAGCT TAGATGCGGG GAAGATAGTT
    CTTGTAGACA CTATACTTAA ACTTGGAAAG GATTATGCAG GAGACACAGC AAGAACATTT
    TCACAGCATG CTGTGAATCA CCTCAAAGTT GCTTACATAC CACCAGCTAA AGACATTGGG
    CCAACTCCTC TACATGTCCA GTTTATATTA TCAGTGAGTA ACCAACATGG AGGAACAGTT
    CAAGGCATCT GTTTCAATAT CACTATCCTT CCAATAGACA ATCAGCCTCC TGAGGTGTTT
    TCATATGATT TGAAAGTTGA AGAAGGTGGT TTCAGCCAAA TTACTGCAAG TAACATTCAC
    ATCACAGATA AGGACACCAG ACAAGAACAA ATTAAAGTTA TTTTACACAA AGCTCCACTG
    CATGGTATCG TTCAACTTGA TGGATCTGCA TTGCAGGAAG GTGACCCATT CACCTGCTTG
    GACCTCATCA CATTAAAAGT ACCTTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48724
    Clone ID Related Accession (Same CDS sequence) XM_031890523.1
    Accession Version XM_031890523.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4473bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product FRAS1-related extracellular matrix protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAGCCTT CTATTGTATA CCCAGTTTTG CTGTCAGTGT TGCTCACAAG GGGCCTCTGC 
    GCCTCCTTTA TCAAAGTCAA TCACGGAATG AAAGTTATGA AAGGACAGAG TGCATTCCTC
    TCAGAAAGTG ACCTGCAGTT TTCCATCCCA AGGGAAAAGG ATGCCTGCAA AGTGGAAGTA
    GTTGTTAATG AACCCATTAC ACAGAGAGTT GGAAGGTTAA CACCACAGGT GTTTGACTGC
    CATTTCCTAG CCAATGAAGT TCAGTACACA CACAATGGCT GCCCTATCTT GGATGAAGAC
    GAGGTCATGC TCAGACTTTA CAGATTTACA GAAACTGAGA CTTTTACTGA AACCTTCCTG
    CTGAGAGTGA AGTTAATAGA GCCTGACTGT AATATCATAC AGCTGGGGCC TAAAAAGCTT
    GAAGTGCCAG AGTTTTATGG TCTGTCTGAC GTTATTGATA AGAACATACT GAGCTTTGAC
    TATGACAAAA GAATAAACAT TGAATGTACC ATCAGAATAT CGTCTTCTGA ATCTTTCCTT
    CCTGCACACG GTCAGCTTGT GACGGGGGAT CCTGTGAAAC AAGAACCACG AGGAGATCAG
    CCACACAGCT TCTTCCATGG ATCAAATCAA AAGCCAGGAC CTTTGTGCAA GAATGGAGGC
    TGCATACAAG GTTTAAAGAG GATCCAGAGC GTTATAAAAA TGAGTTGTGA AGATTTCCTT
    GTAAAGGGCA TCCGATACCA GCACTTGGAT CCTCCATCTC CAAATGTTGA TTATATCAGT
    ATCAGGTTGG ATCTTACAGA CTCAAGGAGC AAATCACTTT ACAAGTCAGA ACATGCTTGG
    ATTCCTGTTC TCATTCGGGA TGCTGTTCCA AATCAAATCC CCAAAGCCAA CTTCATGTCT
    ATGTTTATCT TAGAAGTAGA TCAGTTTGTT CTAACACCCA TCTCAACTGC AACACTGGAT
    GCCGAGGACA GCGAAACAGC CAAACATCTT CTGGTCTTCA ATATTACTTT ACCTCCAAGC
    AAAGGGTTCA TCACACACTT GTCTGACCAT ACCAAGCCCA TTACATCCTT TACATGGAAA
    GATCTCAATG AAATGCTCAT AGCATATCAG CCTCCGAACA GCAGCCACAC AGAGAGAAGA
    AATTTGGAGG TGGAGTTTGA GGTGCATGAT TTCTTTTTTG AAAAGAGTCC TCCAATTACA
    GTGCATATCT CTATACGTAC GGCTGACACC AATGCTCCTC GCGTTTCATG GAACATGGGT
    CTGGATTTAC TGGAGGGCCA GTCTCGACCA ATTACATGGA AACAGTTCCA AATTGTGGAT
    AATGACAACA TCAATGCAGT TCGTTTGGTC ATTGTAGATG GATTGCAGCA TGGACGTATT
    ACTCTGAGAG GTGGGAAAGG ATTTATATTC ACAGTTAAGG ACATCCAGGC TGGAGATGTT
    TGCTACCATC ATGATGACAG TGACACTACT AAGGATTTTA TAGTCTTCAG AATATTTGAT
    GGACGTCACA GCATACGACA CAAGTTTCCT ATTAACATCC TACCAAAAGA TGATAGCCCT
    CCCTTCCTCA TCACAAACAT TGTTATTGAG CTTAGTGAAG GTCAAGCTGT CCTTATCGAG
    GGATCTATGC TTTGTGCTTC TGATATGGAC TCTAGTGATG CATACATACG TTTTAATATC
    ACTAAGCCTT CTGTAGCTGG AGAGATTATA AAGAAGCCAG GACAAAATCT TATTGCTTAT
    CCTGTCACAA GCTTCCTGCA ACGAGACTTA TATAACGGTT TCATTTATTA TCGCCATCTT
    GGAGGAGAAG TATTTGAAGA TTCATTTGAT GTTGTTCTGT CTGATAGCCA TGATCCACCA
    AACTTTTCTG GTCCTCAGGC AGTAATCATT CATATTAGCC CCGTGGATGA CCAGCTGCCC
    ATAGAAGCAC CTGGAACAGT ACGACATCTG ATGGTAAAGG AGAATGAGGT TGTTTATATA
    ACAAAGAAAC AACTGCATTT CATAGACCCA GAGTCACCTG AAAGAGAACT TATTTACACA
    GTTGCTACTC CTCCTTGTAT GGTGCCAACC TACAGCAGCT TAGATGCGGG GAAGATAGTT
    CTTGTAGACA CTATACTTAA ACTTGGAAAG GATTATGCAG GAGACACAGC AAGAACATTT
    TCACAGCATG CTGTGAATCA CCTCAAAGTT GCTTACATAC CACCAGCTAA AGACATTGGG
    CCAACTCCTC TACATGTCCA GTTTATATTA TCAGTGAGTA ACCAACATGG AGGAACAGTT
    CAAGGCATCT GTTTCAATAT CACTATCCTT CCAATAGACA ATCAGCCTCC TGAGGTGTTT
    TCATATGATT TGAAAGTTGA AGAAGGTGGT TTCAGCCAAA TTACTGCAAG TAACATTCAC
    ATCACAGATA AGGACACCAG ACAAGAACAA ATTAAAGTTA TTTTACACAA AGCTCCACTG
    CATGGTATCG TTCAACTTGA TGGATCTGCA TTGCAGGAAG GTGACCCATT CACCTGCTTG
    GACCTCATCA CATTAAAAGT TAGGTACCTT CATGATGGTT CAGAGACCCT ACAAGATGAA
    ATCTCATTTA TTGCAACAGA TGGAATCAAT TCATCTGAGT TTATTTTAAA AATACAGGTT
    TCTCCTATTA ATGATGAACC TCCATTTATT AAAGAGAACC TAATTTCCAC CATTATTTGC
    AAAGAAGGGG CAGAGGTAGT CATATCAGCA GACTATATCT CAGCTACTGA TGCAGACAGT
    GATGACATGA AGCTGATATT TATGATCGCT CGCCAACCAC GTTATGGCAT TTTGCTAAAG
    GCTGGATCTA TCATTGATCA ATTCACCCAG AGAGATGTTG TTGAAGGACT GGTACATTAT
    AGACATACAA GTGGCGAAAT TGGCTTGAAG GTCTATGTTG ATATTATAAC ACTGGTGGTT
    TCTGATGCTG AAGCAGATAA ACCGCAGAAA AAGTGCTGCA ACAATGGTCC TCGTCTGCCG
    ATTTTGCCAA ACCATGAGTC TTTTCCTGTA TATGATCTCA ATGTTACAAT TCTGCCAGTG
    GACAACCAAC CCCCATCGGT TATAATAGGT GAAATATTTG TAGTCGATGA GGGCAAATCA
    TCTGCCCTCA GTGTAGATCA TTTAAGTGCC ATGGACCCTG ACACAGAAAG TAACAATCTG
    GAATTCATAC TGGTTTCTCC TCCTCGATAT GGTTACATTG AAAATGTCCT CCCATCTCCT
    GGATTTGAGA AGAGTAATAT GGGTATAAGT ATTGCTGCAT TTCTACTCAA GGACATTAAA
    TCTCTGAACA TCAATTATGT CCAGTCCCGC CATCAAAGAA TTGAACCAAC CACTGATCAG
    TTTACACTGT ATGTCACTGA TGGCAAACTG CGTTCTGTAG AAAAGCCATT TTACATCGTA
    ATCAATCCGA CAAATGATGA GGTTCCGGAA TTTTTAGCCA GAAACATAAC AGTTGTTGAA
    GGACAAATGA AGAAATTGGA TCCCACTGTA ATAAATGTGT CAGACATGGA TGTGCCACAG
    AACCACTTAT ACTTTACTGT TATAGAGCAG CCCCGACATG GACTGATTAT TGAGGAATCG
    TATATCAGTG GGCACATGAA TAGAAGCCAA TTAATAAAAA GGCATCAGGC AAACAGCCTG
    CCTGTGATAC AAGATTTTTC CATGGACCTG CTAAAAAATG GAATGAAGAT GATATACATG
    CATGATGACT CAGAAAACCT TGCTGACAGC TTCACTATCC AGCTATCTGA TGGAAAGCAT
    GAAGTCAGGA GAACTATTTT TGTGAATGTA ATTGCAGTTA ATGACGAAAA GCCTGTGCTA
    ACCAAGCACT GCGATTTGGT GCTAAGCATG GGGGAACAAA CTCTTATATC AAGTGCCATA
    CTGTCAGCAG AAGATATGGA CACACCCAGG ACAAACATTT CCTATGTTAT TGACAGATGT
    CCAGAGCATG GCCAGCTTCA GATGAAAGTC AATAAAAACT GGGTGACCTT GCATACTGGA
    ATGAAATGCA CTCAGGAGGA CATTGATATG AACCTGGTTA GATATGTGCA TACTGGAACA
    ATGGGCTCAA AAAATGAAGA CACGTTTACT TTCCACCTTT GGGATGGCGG AAACCGATCA
    CCTGAGATAT CTGTTCATAT TTATGTCAAG GATATGGAAA AAGGAGATAT TGCAGTTTTT
    GTGAAAATGC TTAATGTGTC CAAGGGAGAG AGAGTTATCA TAACAACAAA TGTCCTTCTC
    GCCACTGATG GGACCGACAG ACCAGAAGAG CTGCTGTATG TGTTATCCTC GCCACCCCAA
    TATGGGCAGA TTGAATATGT TAAGCATCCT GGAATCCCCA TAACAAGTTT CAGTCAGATG
    GATGTGGCTG CTCACACAAT CTGCTACGTC CACGATTTCA ATTCAGCAGC CACTACTGAT
    ATGTTTAGAT GGGGAAACAA GAACCAATGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031746383.1
    CDS618..5090
    Translation

    Target ORF information:

    RefSeq Version XM_031890523.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis FRAS1 related extracellular matrix 1 (frem1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031890523.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAGCCTT CTATTGTATA CCCAGTTTTG CTGTCAGTGT TGCTCACAAG GGGCCTCTGC 
    GCCTCCTTTA TCAAAGTCAA TCACGGAATG AAAGTTATGA AAGGACAGAG TGCATTCCTC
    TCAGAAAGTG ACCTGCAGTT TTCCATCCCA AGGGAAAAGG ATGCCTGCAA AGTGGAAGTA
    GTTGTTAATG AACCCATTAC ACAGAGAGTT GGAAGGTTAA CACCACAGGT GTTTGACTGC
    CATTTCCTAG CCAATGAAGT TCAGTACACA CACAATGGCT GCCCTATCTT GGATGAAGAC
    GAGGTCATGC TCAGACTTTA CAGATTTACA GAAACTGAGA CTTTTACTGA AACCTTCCTG
    CTGAGAGTGA AGTTAATAGA GCCTGACTGT AATATCATAC AGCTGGGGCC TAAAAAGCTT
    GAAGTGCCAG AGTTTTATGG TCTGTCTGAC GTTATTGATA AGAACATACT GAGCTTTGAC
    TATGACAAAA GAATAAACAT TGAATGTACC ATCAGAATAT CGTCTTCTGA ATCTTTCCTT
    CCTGCACACG GTCAGCTTGT GACGGGGGAT CCTGTGAAAC AAGAACCACG AGGAGATCAG
    CCACACAGCT TCTTCCATGG ATCAAATCAA AAGCCAGGAC CTTTGTGCAA GAATGGAGGC
    TGCATACAAG GTTTAAAGAG GATCCAGAGC GTTATAAAAA TGAGTTGTGA AGATTTCCTT
    GTAAAGGGCA TCCGATACCA GCACTTGGAT CCTCCATCTC CAAATGTTGA TTATATCAGT
    ATCAGGTTGG ATCTTACAGA CTCAAGGAGC AAATCACTTT ACAAGTCAGA ACATGCTTGG
    ATTCCTGTTC TCATTCGGGA TGCTGTTCCA AATCAAATCC CCAAAGCCAA CTTCATGTCT
    ATGTTTATCT TAGAAGTAGA TCAGTTTGTT CTAACACCCA TCTCAACTGC AACACTGGAT
    GCCGAGGACA GCGAAACAGC CAAACATCTT CTGGTCTTCA ATATTACTTT ACCTCCAAGC
    AAAGGGTTCA TCACACACTT GTCTGACCAT ACCAAGCCCA TTACATCCTT TACATGGAAA
    GATCTCAATG AAATGCTCAT AGCATATCAG CCTCCGAACA GCAGCCACAC AGAGAGAAGA
    AATTTGGAGG TGGAGTTTGA GGTGCATGAT TTCTTTTTTG AAAAGAGTCC TCCAATTACA
    GTGCATATCT CTATACGTAC GGCTGACACC AATGCTCCTC GCGTTTCATG GAACATGGGT
    CTGGATTTAC TGGAGGGCCA GTCTCGACCA ATTACATGGA AACAGTTCCA AATTGTGGAT
    AATGACAACA TCAATGCAGT TCGTTTGGTC ATTGTAGATG GATTGCAGCA TGGACGTATT
    ACTCTGAGAG GTGGGAAAGG ATTTATATTC ACAGTTAAGG ACATCCAGGC TGGAGATGTT
    TGCTACCATC ATGATGACAG TGACACTACT AAGGATTTTA TAGTCTTCAG AATATTTGAT
    GGACGTCACA GCATACGACA CAAGTTTCCT ATTAACATCC TACCAAAAGA TGATAGCCCT
    CCCTTCCTCA TCACAAACAT TGTTATTGAG CTTAGTGAAG GTCAAGCTGT CCTTATCGAG
    GGATCTATGC TTTGTGCTTC TGATATGGAC TCTAGTGATG CATACATACG TTTTAATATC
    ACTAAGCCTT CTGTAGCTGG AGAGATTATA AAGAAGCCAG GACAAAATCT TATTGCTTAT
    CCTGTCACAA GCTTCCTGCA ACGAGACTTA TATAACGGTT TCATTTATTA TCGCCATCTT
    GGAGGAGAAG TATTTGAAGA TTCATTTGAT GTTGTTCTGT CTGATAGCCA TGATCCACCA
    AACTTTTCTG GTCCTCAGGC AGTAATCATT CATATTAGCC CCGTGGATGA CCAGCTGCCC
    ATAGAAGCAC CTGGAACAGT ACGACATCTG ATGGTAAAGG AGAATGAGGT TGTTTATATA
    ACAAAGAAAC AACTGCATTT CATAGACCCA GAGTCACCTG AAAGAGAACT TATTTACACA
    GTTGCTACTC CTCCTTGTAT GGTGCCAACC TACAGCAGCT TAGATGCGGG GAAGATAGTT
    CTTGTAGACA CTATACTTAA ACTTGGAAAG GATTATGCAG GAGACACAGC AAGAACATTT
    TCACAGCATG CTGTGAATCA CCTCAAAGTT GCTTACATAC CACCAGCTAA AGACATTGGG
    CCAACTCCTC TACATGTCCA GTTTATATTA TCAGTGAGTA ACCAACATGG AGGAACAGTT
    CAAGGCATCT GTTTCAATAT CACTATCCTT CCAATAGACA ATCAGCCTCC TGAGGTGTTT
    TCATATGATT TGAAAGTTGA AGAAGGTGGT TTCAGCCAAA TTACTGCAAG TAACATTCAC
    ATCACAGATA AGGACACCAG ACAAGAACAA ATTAAAGTTA TTTTACACAA AGCTCCACTG
    CATGGTATCG TTCAACTTGA TGGATCTGCA TTGCAGGAAG GTGACCCATT CACCTGCTTG
    GACCTCATCA CATTAAAAGT TAGGTACCTT CATGATGGTT CAGAGACCCT ACAAGATGAA
    ATCTCATTTA TTGCAACAGA TGGAATCAAT TCATCTGAGT TTATTTTAAA AATACAGGTT
    TCTCCTATTA ATGATGAACC TCCATTTATT AAAGAGAACC TAATTTCCAC CATTATTTGC
    AAAGAAGGGG CAGAGGTAGT CATATCAGCA GACTATATCT CAGCTACTGA TGCAGACAGT
    GATGACATGA AGCTGATATT TATGATCGCT CGCCAACCAC GTTATGGCAT TTTGCTAAAG
    GCTGGATCTA TCATTGATCA ATTCACCCAG AGAGATGTTG TTGAAGGACT GGTACATTAT
    AGACATACAA GTGGCGAAAT TGGCTTGAAG GTCTATGTTG ATATTATAAC ACTGGTGGTT
    TCTGATGCTG AAGCAGATAA ACCGCAGAAA AAGTGCTGCA ACAATGGTCC TCGTCTGCCG
    ATTTTGCCAA ACCATGAGTC TTTTCCTGTA TATGATCTCA ATGTTACAAT TCTGCCAGTG
    GACAACCAAC CCCCATCGGT TATAATAGGT GAAATATTTG TAGTCGATGA GGGCAAATCA
    TCTGCCCTCA GTGTAGATCA TTTAAGTGCC ATGGACCCTG ACACAGAAAG TAACAATCTG
    GAATTCATAC TGGTTTCTCC TCCTCGATAT GGTTACATTG AAAATGTCCT CCCATCTCCT
    GGATTTGAGA AGAGTAATAT GGGTATAAGT ATTGCTGCAT TTCTACTCAA GGACATTAAA
    TCTCTGAACA TCAATTATGT CCAGTCCCGC CATCAAAGAA TTGAACCAAC CACTGATCAG
    TTTACACTGT ATGTCACTGA TGGCAAACTG CGTTCTGTAG AAAAGCCATT TTACATCGTA
    ATCAATCCGA CAAATGATGA GGTTCCGGAA TTTTTAGCCA GAAACATAAC AGTTGTTGAA
    GGACAAATGA AGAAATTGGA TCCCACTGTA ATAAATGTGT CAGACATGGA TGTGCCACAG
    AACCACTTAT ACTTTACTGT TATAGAGCAG CCCCGACATG GACTGATTAT TGAGGAATCG
    TATATCAGTG GGCACATGAA TAGAAGCCAA TTAATAAAAA GGCATCAGGC AAACAGCCTG
    CCTGTGATAC AAGATTTTTC CATGGACCTG CTAAAAAATG GAATGAAGAT GATATACATG
    CATGATGACT CAGAAAACCT TGCTGACAGC TTCACTATCC AGCTATCTGA TGGAAAGCAT
    GAAGTCAGGA GAACTATTTT TGTGAATGTA ATTGCAGTTA ATGACGAAAA GCCTGTGCTA
    ACCAAGCACT GCGATTTGGT GCTAAGCATG GGGGAACAAA CTCTTATATC AAGTGCCATA
    CTGTCAGCAG AAGATATGGA CACACCCAGG ACAAACATTT CCTATGTTAT TGACAGATGT
    CCAGAGCATG GCCAGCTTCA GATGAAAGTC AATAAAAACT GGGTGACCTT GCATACTGGA
    ATGAAATGCA CTCAGGAGGA CATTGATATG AACCTGGTTA GATATGTGCA TACTGGAACA
    ATGGGCTCAA AAAATGAAGA CACGTTTACT TTCCACCTTT GGGATGGCGG AAACCGATCA
    CCTGAGATAT CTGTTCATAT TTATGTCAAG GATATGGAAA AAGGAGATAT TGCAGTTTTT
    GTGAAAATGC TTAATGTGTC CAAGGGAGAG AGAGTTATCA TAACAACAAA TGTCCTTCTC
    GCCACTGATG GGACCGACAG ACCAGAAGAG CTGCTGTATG TGTTATCCTC GCCACCCCAA
    TATGGGCAGA TTGAATATGT TAAGCATCCT GGAATCCCCA TAACAAGTTT CAGTCAGATG
    GATGTGGCTG CTCACACAAT CTGCTACGTC CACGATTTCA ATTCAGCAGC CACTACTGAT
    ATGTTTAGAT GGGGAAACAA GAACCAATGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa04017
    Clone ID Related Accession (Same CDS sequence) XM_002939090.4 , XM_002939090.5
    Accession Version XM_002939090.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6522bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product FRAS1-related extracellular matrix protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002939090.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGAAGCCTT CTATTGTATA CCCAGTTTTG CTGTCAGTGT TGCTCACAAG GGGCCTCTGC 
    GCCTCCTTTA TCAAAGTCAA TCACGGAATG AAAGTTATGA AAGGACAGAG TGCATTCCTC
    TCAGAAAGTG ACCTGCAGTT TTCCATCCCA AGGGAAAAGG ATGCCTGCAA AGTGGAAGTA
    GTTGTTAATG AACCCATTAC ACAGAGAGTT GGAAGGTTAA CACCACAGGT GTTTGACTGC
    CATTTCCTAG CCAATGAAGT TCAGTACACA CACAATGGCT GCCCTATCTT GGATGAAGAC
    GAGGTCATGC TCAGACTTTA CAGATTTACA GAAACTGAGA CTTTTACTGA AACCTTCCTG
    CTGAGAGTGA AGTTAATAGA GCCTGACTGT AATATCATAC AGCTGGGGCC TAAAAAGCTT
    GAAGTGCCAG AGTTTTATGG TCTGTCTGAC GTTATTGATA AGAACATACT GAGCTTTGAC
    TATGACAAAA GAATAAACAT TGAATGTACC ATCAGAATAT CGTCTTCTGA ATCTTTCCTT
    CCTGCACACG GTCAGCTTGT GACGGGGGAT CCTGTGAAAC AAGAACCACG AGGAGATCAG
    CCACACAGCT TCTTCCATGG ATCAAATCAA AAGCCAGGAC CTTTGTGCAA GAATGGAGGC
    TGCATACAAG GTTTAAAGAG GATCCAGAGC GTTATAAAAA TGAGTTGTGA AGATTTCCTT
    GTAAAGGGCA TCCGATACCA GCACTTGGAT CCTCCATCTC CAAATGTTGA TTATATCAGT
    ATCAGGTTGG ATCTTACAGA CTCAAGGAGC AAATCACTTT ACAAGTCAGA ACATGCTTGG
    ATTCCTGTTC TCATTCGGGA TGCTGTTCCA AATCAAATCC CCAAAGCCAA CTTCATGTCT
    ATGTTTATCT TAGAAGTAGA TCAGTTTGTT CTAACACCCA TCTCAACTGC AACACTGGAT
    GCCGAGGACA GCGAAACAGC CAAACATCTT CTGGTCTTCA ATATTACTTT ACCTCCAAGC
    AAAGGGTTCA TCACACACTT GTCTGACCAT ACCAAGCCCA TTACATCCTT TACATGGAAA
    GATCTCAATG AAATGCTCAT AGCATATCAG CCTCCGAACA GCAGCCACAC AGAGAGAAGA
    AATTTGGAGG TGGAGTTTGA GGTGCATGAT TTCTTTTTTG AAAAGAGTCC TCCAATTACA
    GTGCATATCT CTATACGTAC GGCTGACACC AATGCTCCTC GCGTTTCATG GAACATGGGT
    CTGGATTTAC TGGAGGGCCA GTCTCGACCA ATTACATGGA AACAGTTCCA AATTGTGGAT
    AATGACAACA TCAATGCAGT TCGTTTGGTC ATTGTAGATG GATTGCAGCA TGGACGTATT
    ACTCTGAGAG GTGGGAAAGG ATTTATATTC ACAGTTAAGG ACATCCAGGC TGGAGATGTT
    TGCTACCATC ATGATGACAG TGACACTACT AAGGATTTTA TAGTCTTCAG AATATTTGAT
    GGACGTCACA GCATACGACA CAAGTTTCCT ATTAACATCC TACCAAAAGA TGATAGCCCT
    CCCTTCCTCA TCACAAACAT TGTTATTGAG CTTAGTGAAG GTCAAGCTGT CCTTATCGAG
    GGATCTATGC TTTGTGCTTC TGATATGGAC TCTAGTGATG CATACATACG TTTTAATATC
    ACTAAGCCTT CTGTAGCTGG AGAGATTATA AAGAAGCCAG GACAAAATCT TATTGCTTAT
    CCTGTCACAA GCTTCCTGCA ACGAGACTTA TATAACGGTT TCATTTATTA TCGCCATCTT
    GGAGGAGAAG TATTTGAAGA TTCATTTGAT GTTGTTCTGT CTGATAGCCA TGATCCACCA
    AACTTTTCTG GTCCTCAGGC AGTAATCATT CATATTAGCC CCGTGGATGA CCAGCTGCCC
    ATAGAAGCAC CTGGAACAGT ACGACATCTG ATGGTAAAGG AGAATGAGGT TGTTTATATA
    ACAAAGAAAC AACTGCATTT CATAGACCCA GAGTCACCTG AAAGAGAACT TATTTACACA
    GTTGCTACTC CTCCTTGTAT GGTGCCAACC TACAGCAGCT TAGATGCGGG GAAGATAGTT
    CTTGTAGACA CTATACTTAA ACTTGGAAAG GATTATGCAG GAGACACAGC AAGAACATTT
    TCACAGCATG CTGTGAATCA CCTCAAAGTT GCTTACATAC CACCAGCTAA AGACATTGGG
    CCAACTCCTC TACATGTCCA GTTTATATTA TCAGTGAGTA ACCAACATGG AGGAACAGTT
    CAAGGCATCT GTTTCAATAT CACTATCCTT CCAATAGACA ATCAGCCTCC TGAGGTGTTT
    TCATATGATT TGAAAGTTGA AGAAGGTGGT TTCAGCCAAA TTACTGCAAG TAACATTCAC
    ATCACAGATA AGGACACCAG ACAAGAACAA ATTAAAGTTA TTTTACACAA AGCTCCACTG
    CATGGTATCG TTCAACTTGA TGGATCTGCA TTGCAGGAAG GTGACCCATT CACCTGCTTG
    GACCTCATCA CATTAAAAGT TAGGTACCTT CATGATGGTT CAGAGACCCT ACAAGATGAA
    ATCTCATTTA TTGCAACAGA TGGAATCAAT TCATCTGAGT TTATTTTAAA AATACAGGTT
    TCTCCTATTA ATGATGAACC TCCATTTATT AAAGAGAACC TAATTTCCAC CATTATTTGC
    AAAGAAGGGG CAGAGGTAGT CATATCAGCA GACTATATCT CAGCTACTGA TGCAGACAGT
    GATGACATGA AGCTGATATT TATGATCGCT CGCCAACCAC GTTATGGCAT TTTGCTAAAG
    GCTGGATCTA TCATTGATCA ATTCACCCAG AGAGATGTTG TTGAAGGACT GGTACATTAT
    AGACATACAA GTGGCGAAAT TGGCTTGAAG GTCTATGTTG ATATTATAAC ACTGGTGGTT
    TCTGATGCTG AAGCAGATAA ACCGCAGAAA AAGTGCTGCA ACAATGGTCC TCGTCTGCCG
    ATTTTGCCAA ACCATGAGTC TTTTCCTGTA TATGATCTCA ATGTTACAAT TCTGCCAGTG
    GACAACCAAC CCCCATCGGT TATAATAGGT GAAATATTTG TAGTCGATGA GGGCAAATCA
    TCTGCCCTCA GTGTAGATCA TTTAAGTGCC ATGGACCCTG ACACAGAAAG TAACAATCTG
    GAATTCATAC TGGTTTCTCC TCCTCGATAT GGTTACATTG AAAATGTCCT CCCATCTCCT
    GGATTTGAGA AGAGTAATAT GGGTATAAGT ATTGCTGCAT TTCTACTCAA GGACATTAAA
    TCTCTGAACA TCAATTATGT CCAGTCCCGC CATCAAAGAA TTGAACCAAC CACTGATCAG
    TTTACACTGT ATGTCACTGA TGGCAAACTG CGTTCTGTAG AAAAGCCATT TTACATCGTA
    ATCAATCCGA CAAATGATGA GGTTCCGGAA TTTTTAGCCA GAAACATAAC AGTTGTTGAA
    GGACAAATGA AGAAATTGGA TCCCACTGTA ATAAATGTGT CAGACATGGA TGTGCCACAG
    AACCACTTAT ACTTTACTGT TATAGAGCAG CCCCGACATG GACTGATTAT TGAGGAATCG
    TATATCAGTG GGCACATGAA TAGAAGCCAA TTAATAAAAA GGCATCAGGC AAACAGCCTG
    CCTGTGATAC AAGATTTTTC CATGGACCTG CTAAAAAATG GAATGAAGAT GATATACATG
    CATGATGACT CAGAAAACCT TGCTGACAGC TTCACTATCC AGCTATCTGA TGGAAAGCAT
    GAAGTCAGGA GAACTATTTT TGTGAATGTA ATTGCAGTTA ATGACGAAAA GCCTGTGCTA
    ACCAAGCACT GCGATTTGGT GCTAAGCATG GGGGAACAAA CTCTTATATC AAGTGCCATA
    CTGTCAGCAG AAGATATGGA CACACCCAGG ACAAACATTT CCTATGTTAT TGACAGATGT
    CCAGAGCATG GCCAGCTTCA GATGAAAGTC AATAAAAACT GGGTGACCTT GCATACTGGA
    ATGAAATGCA CTCAGGAGGA CATTGATATG AACCTGGTTA GATATGTGCA TACTGGAACA
    ATGGGCTCAA AAAATGAAGA CACGTTTACT TTCCACCTTT GGGATGGCGG AAACCGATCA
    CCTGAGATAT CTGTTCATAT TTATGTCAAG GATATGGAAA AAGGAGATAT TGCAGTTTTT
    GTGAAAATGC TTAATGTGTC CAAGGGAGAG AGAGTTATCA TAACAACAAA TGTCCTTCTC
    GCCACTGATG GGACCGACAG ACCAGAAGAG CTGCTGTATG TGTTATCCTC GCCACCCCAA
    TATGGGCAGA TTGAATATGT TAAGCATCCT GGAATCCCCA TAACAAGTTT CAGTCAGATG
    GATGTGGCTG CTCACACAAT CTGCTACGTC CACGATTTCA ATTCAGCAGC CACTACTGAT
    ATGTTTAGAT TCCTAGTCAG CAATGGGCTG AAAACGAAAC ATGGGATCAT GAACATCGCA
    CTGGAAACAG TCGATCGAAC TCTTCCAACA CTATCCAGGA ACGATGGTCT AAGACAAGTG
    AGAGGGTCTA TGGAGTGTAT TTCCCCGGCC ACCCTACAGG CACTGGACCC TGACACGTCA
    TCTCAGAACA TTACTTTTCA GTTGGTGGGT CTCCCTCAAT ATGGGCAATT ATACCTCAGA
    GGCTTTCCAC TACAGCAAGA TCACTTCACC CAAAGAGATA TTGACAATCT AGACATTGCC
    TATAAGCATG CTGGACAGGA TGCTCAAATA GACAGGTTTA TGTTTACTGT TACAGACAAA
    GCAAATCAAG GATTTATCAT TAATGGGAAG ATTCAGACGG AACCAGTTCC ATTCACAATA
    CAGGTAGATC CTGTGGACAG CACAGCTCCC AGAATTGTCC ATCTTCAATG CCTTACAGAA
    GTTGAGCTTC TGAAAAATGG CCAGTATGGA ATTTATATTA CTTCAAGACA CTTAAAGGCA
    TCTGATCCAG ACACAGATGA TGAAAGAATT GTCTTTCACA TATTAGGAGG GCCTCACTAT
    GGTTATTTAG AGAATGTTAC AACAGGCAGG TTTATAAGAG AAAGATTTAC ACAAAGGGAT
    TTAAACAGCA AATCAGTGCT CTACGTAATA AATCCATCAG TGGATGTTTT TCATGACAAC
    ATAGAGTTTG AAGTTTCAGA CCCCGTTGGA AACAGAGCTT TACCACAAAT TTTAGAGCTG
    AAATGGAGTC AAATTGAATT GCTCCAAGGC ATGTATGAGG TGTGTGAAAC CGAGGCAGTT
    GTGTCTTTGA AGATGACAAG AAAAGGTTAT CTCCTGGAGT CTGCTTTTGT TTCGCTCAAG
    GTGAATGAAA TAAGTGCTGT TCTTGGCAAA GACATTTCCA TGAATCCTTC CAGACTTATA
    CAGTTTGATC CAGGAGTGTC AACCAAGACT TGGAATATAG TAATTATCCA GGATGGATTA
    GAAGAAGATG ACGAGGTCTT TGAAGTAGTT CTGAGTTCTC CTGTGAATGC TGTTCTTGGC
    TCTAAGACAA GAGCTGTAGT GAAAATTGTG GACCCCAGAG GAGGTGGCTG CAGTTCCTCC
    AAAGTCAGCA TGATCAAAAC AACTGGAATG GGCTTACTAA ACTGTACCGT CAAACCCTCA
    CGCAAACCTT GTGCTGTTTT TAGTTTTAAA GAGACTGCAG ATAAGGTCAG GGAAGATGTC
    CAAGAAGATG TCCATACCTT ACAACTTCCG AGGAAGAGAC TAAGACTCAC AGGGAACAGA
    AAAACAGTTA AACCATCATC AGTATTTAGA AACAAAACAG ACATTATTTA TCAATATCAT
    GGGATTGTGT CTCTCCGAGT GGAAGAAGAT AAGCCAGTAT TAAATGAAAC AAAGAAAGCA
    AAAGTACTAG TGAAGAACCG TGGTCAGAGG AAGATAAATG CCCCCTCCCA AAATCCTGAC
    ATTCTTCAAT CAGGCAGAAC CATGTTGGAT TCTCATGTTA CTACTGGTGG TCAGGATAAC
    GCAATATCGT TTCCTAAAAG ATGCACACCA AGTTTAAAAG GACTCCTGCA TTTTGAGGAC
    ACAGTACAGA AACTGTTCCA GTGTGACGGC ATATCGTGGA ACCCCTGGAT CACTACTAAG
    GATTCTAAAG CAGGAAAATG CCCATTTGGC TGGACTTACC ATGATGGCTA CTGCTATATA
    CTGATCAATG ATCAAAAGGC CACCTGGATA ACAGCAGCCA GAGCATGTAG AGAACAGCAT
    TTTGCTACTC TGGTGGATGT ATTCAGCAAG GAACAAATGG AATGGCTTTG GAATTTCAGT
    GGCAGAAAAT CCTTTTGGAT AGGCCTCAAT GATAGAGTAC GTAATGGAAA CTGGGAGTGG
    AGCAAAGGGG AAATGGTCAC CTTCACCAAT TGGAAAAGAG GCTCTCCTCG ATCTTCACAC
    AAAGGGAAGA ATTGCGTTTT GGCACAAAAG AGAGGCAAAT GGCAGGCTAA AGACTGCAAA
    AAAGGCAAAG GCCATTACTA TATATGTTCA AAAAAGTTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002939136.2
    CDS613..7134
    Translation

    Target ORF information:

    RefSeq Version XM_002939090.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis FRAS1 related extracellular matrix 1 (frem1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002939090.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGAAGCCTT CTATTGTATA CCCAGTTTTG CTGTCAGTGT TGCTCACAAG GGGCCTCTGC 
    GCCTCCTTTA TCAAAGTCAA TCACGGAATG AAAGTTATGA AAGGACAGAG TGCATTCCTC
    TCAGAAAGTG ACCTGCAGTT TTCCATCCCA AGGGAAAAGG ATGCCTGCAA AGTGGAAGTA
    GTTGTTAATG AACCCATTAC ACAGAGAGTT GGAAGGTTAA CACCACAGGT GTTTGACTGC
    CATTTCCTAG CCAATGAAGT TCAGTACACA CACAATGGCT GCCCTATCTT GGATGAAGAC
    GAGGTCATGC TCAGACTTTA CAGATTTACA GAAACTGAGA CTTTTACTGA AACCTTCCTG
    CTGAGAGTGA AGTTAATAGA GCCTGACTGT AATATCATAC AGCTGGGGCC TAAAAAGCTT
    GAAGTGCCAG AGTTTTATGG TCTGTCTGAC GTTATTGATA AGAACATACT GAGCTTTGAC
    TATGACAAAA GAATAAACAT TGAATGTACC ATCAGAATAT CGTCTTCTGA ATCTTTCCTT
    CCTGCACACG GTCAGCTTGT GACGGGGGAT CCTGTGAAAC AAGAACCACG AGGAGATCAG
    CCACACAGCT TCTTCCATGG ATCAAATCAA AAGCCAGGAC CTTTGTGCAA GAATGGAGGC
    TGCATACAAG GTTTAAAGAG GATCCAGAGC GTTATAAAAA TGAGTTGTGA AGATTTCCTT
    GTAAAGGGCA TCCGATACCA GCACTTGGAT CCTCCATCTC CAAATGTTGA TTATATCAGT
    ATCAGGTTGG ATCTTACAGA CTCAAGGAGC AAATCACTTT ACAAGTCAGA ACATGCTTGG
    ATTCCTGTTC TCATTCGGGA TGCTGTTCCA AATCAAATCC CCAAAGCCAA CTTCATGTCT
    ATGTTTATCT TAGAAGTAGA TCAGTTTGTT CTAACACCCA TCTCAACTGC AACACTGGAT
    GCCGAGGACA GCGAAACAGC CAAACATCTT CTGGTCTTCA ATATTACTTT ACCTCCAAGC
    AAAGGGTTCA TCACACACTT GTCTGACCAT ACCAAGCCCA TTACATCCTT TACATGGAAA
    GATCTCAATG AAATGCTCAT AGCATATCAG CCTCCGAACA GCAGCCACAC AGAGAGAAGA
    AATTTGGAGG TGGAGTTTGA GGTGCATGAT TTCTTTTTTG AAAAGAGTCC TCCAATTACA
    GTGCATATCT CTATACGTAC GGCTGACACC AATGCTCCTC GCGTTTCATG GAACATGGGT
    CTGGATTTAC TGGAGGGCCA GTCTCGACCA ATTACATGGA AACAGTTCCA AATTGTGGAT
    AATGACAACA TCAATGCAGT TCGTTTGGTC ATTGTAGATG GATTGCAGCA TGGACGTATT
    ACTCTGAGAG GTGGGAAAGG ATTTATATTC ACAGTTAAGG ACATCCAGGC TGGAGATGTT
    TGCTACCATC ATGATGACAG TGACACTACT AAGGATTTTA TAGTCTTCAG AATATTTGAT
    GGACGTCACA GCATACGACA CAAGTTTCCT ATTAACATCC TACCAAAAGA TGATAGCCCT
    CCCTTCCTCA TCACAAACAT TGTTATTGAG CTTAGTGAAG GTCAAGCTGT CCTTATCGAG
    GGATCTATGC TTTGTGCTTC TGATATGGAC TCTAGTGATG CATACATACG TTTTAATATC
    ACTAAGCCTT CTGTAGCTGG AGAGATTATA AAGAAGCCAG GACAAAATCT TATTGCTTAT
    CCTGTCACAA GCTTCCTGCA ACGAGACTTA TATAACGGTT TCATTTATTA TCGCCATCTT
    GGAGGAGAAG TATTTGAAGA TTCATTTGAT GTTGTTCTGT CTGATAGCCA TGATCCACCA
    AACTTTTCTG GTCCTCAGGC AGTAATCATT CATATTAGCC CCGTGGATGA CCAGCTGCCC
    ATAGAAGCAC CTGGAACAGT ACGACATCTG ATGGTAAAGG AGAATGAGGT TGTTTATATA
    ACAAAGAAAC AACTGCATTT CATAGACCCA GAGTCACCTG AAAGAGAACT TATTTACACA
    GTTGCTACTC CTCCTTGTAT GGTGCCAACC TACAGCAGCT TAGATGCGGG GAAGATAGTT
    CTTGTAGACA CTATACTTAA ACTTGGAAAG GATTATGCAG GAGACACAGC AAGAACATTT
    TCACAGCATG CTGTGAATCA CCTCAAAGTT GCTTACATAC CACCAGCTAA AGACATTGGG
    CCAACTCCTC TACATGTCCA GTTTATATTA TCAGTGAGTA ACCAACATGG AGGAACAGTT
    CAAGGCATCT GTTTCAATAT CACTATCCTT CCAATAGACA ATCAGCCTCC TGAGGTGTTT
    TCATATGATT TGAAAGTTGA AGAAGGTGGT TTCAGCCAAA TTACTGCAAG TAACATTCAC
    ATCACAGATA AGGACACCAG ACAAGAACAA ATTAAAGTTA TTTTACACAA AGCTCCACTG
    CATGGTATCG TTCAACTTGA TGGATCTGCA TTGCAGGAAG GTGACCCATT CACCTGCTTG
    GACCTCATCA CATTAAAAGT TAGGTACCTT CATGATGGTT CAGAGACCCT ACAAGATGAA
    ATCTCATTTA TTGCAACAGA TGGAATCAAT TCATCTGAGT TTATTTTAAA AATACAGGTT
    TCTCCTATTA ATGATGAACC TCCATTTATT AAAGAGAACC TAATTTCCAC CATTATTTGC
    AAAGAAGGGG CAGAGGTAGT CATATCAGCA GACTATATCT CAGCTACTGA TGCAGACAGT
    GATGACATGA AGCTGATATT TATGATCGCT CGCCAACCAC GTTATGGCAT TTTGCTAAAG
    GCTGGATCTA TCATTGATCA ATTCACCCAG AGAGATGTTG TTGAAGGACT GGTACATTAT
    AGACATACAA GTGGCGAAAT TGGCTTGAAG GTCTATGTTG ATATTATAAC ACTGGTGGTT
    TCTGATGCTG AAGCAGATAA ACCGCAGAAA AAGTGCTGCA ACAATGGTCC TCGTCTGCCG
    ATTTTGCCAA ACCATGAGTC TTTTCCTGTA TATGATCTCA ATGTTACAAT TCTGCCAGTG
    GACAACCAAC CCCCATCGGT TATAATAGGT GAAATATTTG TAGTCGATGA GGGCAAATCA
    TCTGCCCTCA GTGTAGATCA TTTAAGTGCC ATGGACCCTG ACACAGAAAG TAACAATCTG
    GAATTCATAC TGGTTTCTCC TCCTCGATAT GGTTACATTG AAAATGTCCT CCCATCTCCT
    GGATTTGAGA AGAGTAATAT GGGTATAAGT ATTGCTGCAT TTCTACTCAA GGACATTAAA
    TCTCTGAACA TCAATTATGT CCAGTCCCGC CATCAAAGAA TTGAACCAAC CACTGATCAG
    TTTACACTGT ATGTCACTGA TGGCAAACTG CGTTCTGTAG AAAAGCCATT TTACATCGTA
    ATCAATCCGA CAAATGATGA GGTTCCGGAA TTTTTAGCCA GAAACATAAC AGTTGTTGAA
    GGACAAATGA AGAAATTGGA TCCCACTGTA ATAAATGTGT CAGACATGGA TGTGCCACAG
    AACCACTTAT ACTTTACTGT TATAGAGCAG CCCCGACATG GACTGATTAT TGAGGAATCG
    TATATCAGTG GGCACATGAA TAGAAGCCAA TTAATAAAAA GGCATCAGGC AAACAGCCTG
    CCTGTGATAC AAGATTTTTC CATGGACCTG CTAAAAAATG GAATGAAGAT GATATACATG
    CATGATGACT CAGAAAACCT TGCTGACAGC TTCACTATCC AGCTATCTGA TGGAAAGCAT
    GAAGTCAGGA GAACTATTTT TGTGAATGTA ATTGCAGTTA ATGACGAAAA GCCTGTGCTA
    ACCAAGCACT GCGATTTGGT GCTAAGCATG GGGGAACAAA CTCTTATATC AAGTGCCATA
    CTGTCAGCAG AAGATATGGA CACACCCAGG ACAAACATTT CCTATGTTAT TGACAGATGT
    CCAGAGCATG GCCAGCTTCA GATGAAAGTC AATAAAAACT GGGTGACCTT GCATACTGGA
    ATGAAATGCA CTCAGGAGGA CATTGATATG AACCTGGTTA GATATGTGCA TACTGGAACA
    ATGGGCTCAA AAAATGAAGA CACGTTTACT TTCCACCTTT GGGATGGCGG AAACCGATCA
    CCTGAGATAT CTGTTCATAT TTATGTCAAG GATATGGAAA AAGGAGATAT TGCAGTTTTT
    GTGAAAATGC TTAATGTGTC CAAGGGAGAG AGAGTTATCA TAACAACAAA TGTCCTTCTC
    GCCACTGATG GGACCGACAG ACCAGAAGAG CTGCTGTATG TGTTATCCTC GCCACCCCAA
    TATGGGCAGA TTGAATATGT TAAGCATCCT GGAATCCCCA TAACAAGTTT CAGTCAGATG
    GATGTGGCTG CTCACACAAT CTGCTACGTC CACGATTTCA ATTCAGCAGC CACTACTGAT
    ATGTTTAGAT TCCTAGTCAG CAATGGGCTG AAAACGAAAC ATGGGATCAT GAACATCGCA
    CTGGAAACAG TCGATCGAAC TCTTCCAACA CTATCCAGGA ACGATGGTCT AAGACAAGTG
    AGAGGGTCTA TGGAGTGTAT TTCCCCGGCC ACCCTACAGG CACTGGACCC TGACACGTCA
    TCTCAGAACA TTACTTTTCA GTTGGTGGGT CTCCCTCAAT ATGGGCAATT ATACCTCAGA
    GGCTTTCCAC TACAGCAAGA TCACTTCACC CAAAGAGATA TTGACAATCT AGACATTGCC
    TATAAGCATG CTGGACAGGA TGCTCAAATA GACAGGTTTA TGTTTACTGT TACAGACAAA
    GCAAATCAAG GATTTATCAT TAATGGGAAG ATTCAGACGG AACCAGTTCC ATTCACAATA
    CAGGTAGATC CTGTGGACAG CACAGCTCCC AGAATTGTCC ATCTTCAATG CCTTACAGAA
    GTTGAGCTTC TGAAAAATGG CCAGTATGGA ATTTATATTA CTTCAAGACA CTTAAAGGCA
    TCTGATCCAG ACACAGATGA TGAAAGAATT GTCTTTCACA TATTAGGAGG GCCTCACTAT
    GGTTATTTAG AGAATGTTAC AACAGGCAGG TTTATAAGAG AAAGATTTAC ACAAAGGGAT
    TTAAACAGCA AATCAGTGCT CTACGTAATA AATCCATCAG TGGATGTTTT TCATGACAAC
    ATAGAGTTTG AAGTTTCAGA CCCCGTTGGA AACAGAGCTT TACCACAAAT TTTAGAGCTG
    AAATGGAGTC AAATTGAATT GCTCCAAGGC ATGTATGAGG TGTGTGAAAC CGAGGCAGTT
    GTGTCTTTGA AGATGACAAG AAAAGGTTAT CTCCTGGAGT CTGCTTTTGT TTCGCTCAAG
    GTGAATGAAA TAAGTGCTGT TCTTGGCAAA GACATTTCCA TGAATCCTTC CAGACTTATA
    CAGTTTGATC CAGGAGTGTC AACCAAGACT TGGAATATAG TAATTATCCA GGATGGATTA
    GAAGAAGATG ACGAGGTCTT TGAAGTAGTT CTGAGTTCTC CTGTGAATGC TGTTCTTGGC
    TCTAAGACAA GAGCTGTAGT GAAAATTGTG GACCCCAGAG GAGGTGGCTG CAGTTCCTCC
    AAAGTCAGCA TGATCAAAAC AACTGGAATG GGCTTACTAA ACTGTACCGT CAAACCCTCA
    CGCAAACCTT GTGCTGTTTT TAGTTTTAAA GAGACTGCAG ATAAGGTCAG GGAAGATGTC
    CAAGAAGATG TCCATACCTT ACAACTTCCG AGGAAGAGAC TAAGACTCAC AGGGAACAGA
    AAAACAGTTA AACCATCATC AGTATTTAGA AACAAAACAG ACATTATTTA TCAATATCAT
    GGGATTGTGT CTCTCCGAGT GGAAGAAGAT AAGCCAGTAT TAAATGAAAC AAAGAAAGCA
    AAAGTACTAG TGAAGAACCG TGGTCAGAGG AAGATAAATG CCCCCTCCCA AAATCCTGAC
    ATTCTTCAAT CAGGCAGAAC CATGTTGGAT TCTCATGTTA CTACTGGTGG TCAGGATAAC
    GCAATATCGT TTCCTAAAAG ATGCACACCA AGTTTAAAAG GACTCCTGCA TTTTGAGGAC
    ACAGTACAGA AACTGTTCCA GTGTGACGGC ATATCGTGGA ACCCCTGGAT CACTACTAAG
    GATTCTAAAG CAGGAAAATG CCCATTTGGC TGGACTTACC ATGATGGCTA CTGCTATATA
    CTGATCAATG ATCAAAAGGC CACCTGGATA ACAGCAGCCA GAGCATGTAG AGAACAGCAT
    TTTGCTACTC TGGTGGATGT ATTCAGCAAG GAACAAATGG AATGGCTTTG GAATTTCAGT
    GGCAGAAAAT CCTTTTGGAT AGGCCTCAAT GATAGAGTAC GTAATGGAAA CTGGGAGTGG
    AGCAAAGGGG AAATGGTCAC CTTCACCAAT TGGAAAAGAG GCTCTCCTCG ATCTTCACAC
    AAAGGGAAGA ATTGCGTTTT GGCACAAAAG AGAGGCAAAT GGCAGGCTAA AGACTGCAAA
    AAAGGCAAAG GCCATTACTA TATATGTTCA AAAAAGTTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa42096
    Clone ID Related Accession (Same CDS sequence) XM_018097904.1
    Accession Version XM_018097904.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6345bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product FRAS1-related extracellular matrix protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGAAGCCTT CTATTGTATA CCCAGTTTTG CTGTCAGTGT TGCTCACAAG GGGCCTCTGC 
    GCCTCCTTTA TCAAAGTCAA TCACGGAATG AAAGTTATGA AAGGACAGAG TGCATTCCTC
    TCAGAAAGTG ACCTGCAGTT TTCCATCCCA AGGGAAAAGG ATGCCTGCAA AGTGGAAGTA
    GTTGTTAATG AACCCATTAC ACAGAGAGTT GGAAGGTTAA CACCACAGGT GTTTGACTGC
    CATTTCCTAG CCAATGAAGT TCAGTACACA CACAATGGCT GCCCTATCTT GGATGAAGAC
    GAGGTCATGC TCAGACTTTA CAGATTTACA GAAACTGAGA CTTTTACTGA AACCTTCCTG
    CTGAGAGTGA AGTTAATAGA GCCTGACTGT AATATCATAC AGCTGGGGCC TAAAAAGCTT
    GAAGTGCCAG AGTTTTATGG TCTGTCTGAC GTTATTGATA AGAACATACT GAGCTTTGAC
    TATGACAAAA GAATAAACAT TGAATGTACC ATCAGAATAT CGTCTTCTGA ATCTTTCCTT
    CCTGCACACG GTCAGCTTGT GACGGGGGAT CCTGTGAAAC AAGAACCACG AGGAGATCAG
    CCACACAGCT TCTTCCATGG ATCAAATCAA AAGCCAGGAC CTTTGTGCAA GAATGGAGGC
    TGCATACAAG GTTTAAAGAG GATCCAGAGC GTTATAAAAA TGAGTTGTGA AGATTTCCTT
    GTAAAGGGCA TCCGATACCA GCACTTGGAT CCTCCATCTC CAAATGTTGA TTATATCAGT
    ATCAGGTTGG ATCTTACAGA CTCAAGGAGC AAATCACTTT ACAAGTCAGA ACATGCTTGG
    ATTCCTGTTC TCATTCGGGA TGCTGTTCCA AATCAAATCC CCAAAGCCAA CTTCATGTCT
    ATGTTTATCT TAGAAGTAGA TCAGTTTGTT CTAACACCCA TCTCAACTGC AACACTGGAT
    GCCGAGGACA GCGAAACAGC CAAACATCTT CTGGTCTTCA ATATTACTTT ACCTCCAAGC
    AAAGGGTTCA TCACACACTT GTCTGACCAT ACCAAGCCCA TTACATCCTT TACATGGAAA
    GATCTCAATG AAATGCTCAT AGCATATCAG CCTCCGAACA GCAGCCACAC AGAGAGAAGA
    AATTTGGAGG TGGAGTTTGA GGTGCATGAT TTCTTTTTTG AAAAGAGTCC TCCAATTACA
    GTGCATATCT CTATACGTAC GGCTGACACC AATGCTCCTC GCGTTTCATG GAACATGGGT
    CTGGATTTAC TGGAGGGCCA GTCTCGACCA ATTACATGGA AACAGTTCCA AATTGTGGAT
    AATGACAACA TCAATGCAGT TCGTTTGGTC ATTGTAGATG GATTGCAGCA TGGACGTATT
    ACTCTGAGAG GTGGGAAAGG ATTTATATTC ACAGTTAAGG ACATCCAGGC TGGAGATGTT
    TGCTACCATC ATGATGACAG TGACACTACT AAGGATTTTA TAGTCTTCAG AATATTTGAT
    GGACGTCACA GCATACGACA CAAGTTTCCT ATTAACATCC TACCAAAAGA TGATAGCCCT
    CCCTTCCTCA TCACAAACAT TGTTATTGAG CTTAGTGAAG GTCAAGCTGT CCTTATCGAG
    GGATCTATGC TTTGTGCTTC TGATATGGAC TCTAGTGATG CATACATACG TTTTAATATC
    ACTAAGCCTT CTGTAGCTGG AGAGATTATA AAGAAGCCAG GACAAAATCT TATTGCTTAT
    CCTGTCACAA GCTTCCTGCA ACGAGACTTA TATAACGGTT TCATTTATTA TCGCCATCTT
    GGAGGAGAAG TATTTGAAGA TTCATTTGAT GTTGTTCTGT CTGATAGCCA TGATCCACCA
    AACTTTTCTG GTCCTCAGGC AGTAATCATT CATATTAGCC CCGTGGATGA CCAGCTGCCC
    ATAGAAGCAC CTGGAACAGT ACGACATCTG ATGGTAAAGG AGAATGAGGT TGTTTATATA
    ACAAAGAAAC AACTGCATTT CATAGACCCA GAGTCACCTG AAAGAGAACT TATTTACACA
    GTTGCTACTC CTCCTTGTAT GGTGCCAACC TACAGCAGCT TAGATGCGGG GAAGATAGTT
    CTTGTAGACA CTATACTTAA ACTTGGAAAG GATTATGCAG GAGACACAGC AAGAACATTT
    TCACAGCATG CTGTGAATCA CCTCAAAGTT GCTTACATAC CACCAGCTAA AGACATTGGG
    CCAACTCCTC TACATGTCCA GTTTATATTA TCAGTGAGTA ACCAACATGG AGGAACAGTT
    CAAGGCATCT GTTTCAATAT CACTATCCTT CCAATAGACA ATCAGCCTCC TGAGGTGTTT
    TCATATGATT TGAAAGTTGA AGAAGGTGGT TTCAGCCAAA TTACTGCAAG TAACATTCAC
    ATCACAGATA AGGACACCAG ACAAGAACAA ATTAAAGTTA TTTTACACAA AGCTCCACTG
    CATGGTATCG TTCAACTTGA TGGATCTGCA TTGCAGGAAG GTGACCCATT CACCTGCTTG
    GACCTCATCA CATTAAAAGT TAGGTACCTT CATGATGGTT CAGAGACCCT ACAAGATGAA
    ATCTCATTTA TTGCAACAGA TGGAATCAAT TCATCTGAGT TTATTTTAAA AATACAGGTT
    TCTCCTATTA ATGATGAACC TCCATTTATT AAAGAGAACC TAATTTCCAC CATTATTTGC
    AAAGAAGGGG CAGAGGTAGT CATATCAGCA GACTATATCT CAGCTACTGA TGCAGACAGT
    GATGACATGA AGCTGATATT TATGATCGCT CGCCAACCAC GTTATGGCAT TTTGCTAAAG
    GCTGGATCTA TCATTGATCA ATTCACCCAG AGAGATGTTG TTGAAGGACT GGTACATTAT
    AGACATACAA GTGGCGAAAT TGGCTTGAAG GTCTATGTTG ATATTATAAC ACTGGTGGTT
    TCTGATGCTG AAGCAGATAA ACCGCAGAAA AAGTGCTGCA ACAATGGTCC TCGTCTGCCG
    ATTTTGCCAA ACCATGAGTC TTTTCCTGTA TATGATCTCA ATGTTACAAT TCTGCCAGTG
    GACAACCAAC CCCCATCGGT TATAATAGGT GAAATATTTG TAGTCGATGA GGGCAAATCA
    TCTGCCCTCA GTGTAGATCA TTTAAGTGCC ATGGACCCTG ACACAGAAAG TAACAATCTG
    GAATTCATAC TGGTTTCTCC TCCTCGATAT GGTTACATTG AAAATGTCCT CCCATCTCCT
    GGATTTGAGA AGAGTAATAT GGGTATAAGT ATTGCTGCAT TTCTACTCAA GGACATTAAA
    TCTCTGAACA TCAATTATGT CCAGTCCCGC CATCAAAGAA TTGAACCAAC CACTGATCAG
    TTTACACTGT ATGTCACTGA TGGCAAACTG CGTTCTGTAG AAAAGCCATT TTACATCGTA
    ATCAATCCGA CAAATGATGA GGTTCCGGAA TTTTTAGCCA GAAACATAAC AGTTGTTGAA
    GGACAAATGA AGAAATTGGA TCCCACTGTA ATAAATGTGT CAGACATGGA TGTGCCACAG
    AACCACTTAT ACTTTACTGT TATAGAGCAG CCCCGACATG GACTGATTAT TGAGGAATCG
    TATATCAGTG GGCACATGAA TAGAAGCCAA TTAATAAAAA GGCATCAGGC AAACAGCCTG
    CCTGTGATAC AAGATTTTTC CATGGACCTG CTAAAAAATG GAATGAAGAT GATATACATG
    CATGATGACT CAGAAAACCT TGCTGACAGC TTCACTATCC AGCTATCTGA TGGAAAGCAT
    GAAGTCAGGA GAACTATTTT TGTGAATGTA ATTGCAGTTA ATGACGAAAA GCCTGTGCTA
    ACCAAGCACT GCGATTTGGT GCTAAGCATG GGGGAACAAA CTCTTATATC AAGTGCCATA
    CTGTCAGCAG AAGATATGGA CACACCCAGG ACAAACATTT CCTATGTTAT TGACAGATGT
    CCAGAGCATG GCCAGCTTCA GATGAAAGTC AATAAAAACT GGGTGACCTT GCATACTGGA
    ATGAAATGCA CTCAGGAGGA CATTGATATG AACCTGGTTA GATATGTGCA TACTGGAACA
    ATGGGCTCAA AAAATGAAGA CACGTTTACT TTCCACCTTT GGGATGGCGG AAACCGATCA
    CCTGAGATAT CTGTTCATAT TTATGTCAAG GATATGGAAA AAGGAGATAT TGCAGTTTTT
    GTGAAAATGC TTAATGTGTC CAAGGGAGAG AGAGTTATCA TAACAACAAA TGTCCTTCTC
    GCCACTGATG GGACCGACAG ACCAGAAGAG CTGCTGTATG TGTTATCCTC GCCACCCCAA
    TATGGGCAGA TTGAATATGT TAAGCATCCT GGAATCCCCA TAACAAGTTT CAGTCAGATG
    GATGTGGCTG CTCACACAAT CTGCTACGTC CACGATTTCA ATTCAGCAGC CACTACTGAT
    ATGTTTAGAT TCCTAGTCAG CAATGGGCTG AAAACGAAAC ATGGGATCAT GAACATCGCA
    CTGGAAACAG TCGATCGAAC TCTTCCAACA CTATCCAGGA ACGATGGTCT AAGACAAGTG
    AGAGGGTCTA TGGAGTGTAT TTCCCCGGCC ACCCTACAGG CACTGGACCC TGACACGTCA
    TCTCAGAACA TTACTTTTCA GTTGGTGGGT CTCCCTCAAT ATGGGCAATT ATACCTCAGA
    GGCTTTCCAC TACAGCAAGA TCACTTCACC CAAAGAGATA TTGACAATCT AGACATTGCC
    TATAAGCATG CTGGACAGGA TGCTCAAATA GACAGGTTTA TGTTTACTGT TACAGACAAA
    GCAAATCAAG GATTTATCAT TAATGGGAAG ATTCAGACGG AACCAGTTCC ATTCACAATA
    CAGGTAGATC CTGTGGACAG CACAGCTCCC AGAATTGTCC ATCTTCAATG CCTTACAGAA
    GTTGAGCTTC TGAAAAATGG CCAGTATGGA ATTTATATTA CTTCAAGACA CTTAAAGGCA
    TCTGATCCAG ACACAGATGA TGAAAGAATT GTCTTTCACA TATTAGGAGG GCCTCACTAT
    GGTTATTTAG AGAATGTTAC AACAGGCAGG TTTATAAGAG AAAGATTTAC ACAAAGGGAT
    TTAAACAGCA AATCAGTGCT CTACGTAATA AATCCATCAG TGGATGTTTT TCATGACAAC
    ATAGAGTTTG AAGTTTCAGA CCCCGTTGGA AACAGAGCTT TACCACAAAT TTTAGAGCTG
    AAATGGAGTC AAATTGAATT GCTCCAAGGC ATGTATGAGG TGTGTGAAAC CGAGGCAGTT
    GTGTCTTTGA AGATGACAAG AAAAGGTTAT CTCCTGGAGT CTGCTTTTGT TTCGCTCAAG
    GTGAATGAAA TAAGTGCTGT TCTTGGCAAA GACATTTCCA TGAATCCTTC CAGACTTATA
    CAGTTTGATC CAGGAGTGTC AACCAAGACT TGGAATATAG TAATTATCCA GGATGGATTA
    GAAGAAGATG ACGAGGTCTT TGAAGTAGTT CTGAGTTCTC CTGTGAATGC TGTTCTTGGC
    TCTAAGACAA GAGCTGTAGT GAAAATTGTG GACCCCAGAG GAGGTGGCTG CAGTTCCTCC
    AAAGTCAGCA TGATCAAAAC AACTGGAATG GGCTTACTAA ACTGTACCGT CAAACCCTCA
    CGCAAACCTT GTGCTGTTTT TAGTTTTAAA GAGACTGCAG ATAAGGTCAG GGAAGATGTC
    CAAGAAGATG TCCATACCTT ACAACTTCCG AGGAAGAGAC TAAGACTCAC AGGGAACAGA
    AAAACAGTTA AACCATCATC AGTATTTAGA AACAAAACAG ACATTATTTA TCAATATCAT
    GGGATTGTGT CTCTCCGAGT GGAAGAAGAT AAGCCAGTAT TAAATGAAAC AAAGAAAGCA
    AAAGTACTAG TGAAGAACCG TGGTCAGAGG AAGATAAATG CCCCCTCCCA AAATCCTGAC
    ATTCTTCAAT CAGGCAGAAC CATGTTGGAT TCTCATGTTA CTACTGGTGG TCAGGATAAC
    GCAATATCGT TTCCTAAAAG ATGCACACCA AGTTTAAAAG GACTCCTGCA TTTTGAGGAC
    ACAGTACAGA AACTGTTCCA GTGTGACGGC ATATCGTGGA ACCCCTGGAT CACTACTAAG
    GATTCTAAAG CAGGAAAATG CCCATTTGGC TGGACTTACC ATGATGGCTA CTGCTATATA
    CTGATCAATG ATCAAAAGGC CACCTGGATA ACAGCAGCCA GAGCATGTAG AGAACAGCAT
    TTTGCTACTC TGGTGGATGT ATTCAGCAAG GAACAAATGG AATGGCTTTG GAATTTCAGT
    GGCAGAAAAT CCTTTTGGAT AGGTGACTAC TTATATTATT ATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017953393.1
    CDS608..6952
    Translation

    Target ORF information:

    RefSeq Version XM_018097904.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis FRAS1 related extracellular matrix 1 (frem1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018097904.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGAAGCCTT CTATTGTATA CCCAGTTTTG CTGTCAGTGT TGCTCACAAG GGGCCTCTGC 
    GCCTCCTTTA TCAAAGTCAA TCACGGAATG AAAGTTATGA AAGGACAGAG TGCATTCCTC
    TCAGAAAGTG ACCTGCAGTT TTCCATCCCA AGGGAAAAGG ATGCCTGCAA AGTGGAAGTA
    GTTGTTAATG AACCCATTAC ACAGAGAGTT GGAAGGTTAA CACCACAGGT GTTTGACTGC
    CATTTCCTAG CCAATGAAGT TCAGTACACA CACAATGGCT GCCCTATCTT GGATGAAGAC
    GAGGTCATGC TCAGACTTTA CAGATTTACA GAAACTGAGA CTTTTACTGA AACCTTCCTG
    CTGAGAGTGA AGTTAATAGA GCCTGACTGT AATATCATAC AGCTGGGGCC TAAAAAGCTT
    GAAGTGCCAG AGTTTTATGG TCTGTCTGAC GTTATTGATA AGAACATACT GAGCTTTGAC
    TATGACAAAA GAATAAACAT TGAATGTACC ATCAGAATAT CGTCTTCTGA ATCTTTCCTT
    CCTGCACACG GTCAGCTTGT GACGGGGGAT CCTGTGAAAC AAGAACCACG AGGAGATCAG
    CCACACAGCT TCTTCCATGG ATCAAATCAA AAGCCAGGAC CTTTGTGCAA GAATGGAGGC
    TGCATACAAG GTTTAAAGAG GATCCAGAGC GTTATAAAAA TGAGTTGTGA AGATTTCCTT
    GTAAAGGGCA TCCGATACCA GCACTTGGAT CCTCCATCTC CAAATGTTGA TTATATCAGT
    ATCAGGTTGG ATCTTACAGA CTCAAGGAGC AAATCACTTT ACAAGTCAGA ACATGCTTGG
    ATTCCTGTTC TCATTCGGGA TGCTGTTCCA AATCAAATCC CCAAAGCCAA CTTCATGTCT
    ATGTTTATCT TAGAAGTAGA TCAGTTTGTT CTAACACCCA TCTCAACTGC AACACTGGAT
    GCCGAGGACA GCGAAACAGC CAAACATCTT CTGGTCTTCA ATATTACTTT ACCTCCAAGC
    AAAGGGTTCA TCACACACTT GTCTGACCAT ACCAAGCCCA TTACATCCTT TACATGGAAA
    GATCTCAATG AAATGCTCAT AGCATATCAG CCTCCGAACA GCAGCCACAC AGAGAGAAGA
    AATTTGGAGG TGGAGTTTGA GGTGCATGAT TTCTTTTTTG AAAAGAGTCC TCCAATTACA
    GTGCATATCT CTATACGTAC GGCTGACACC AATGCTCCTC GCGTTTCATG GAACATGGGT
    CTGGATTTAC TGGAGGGCCA GTCTCGACCA ATTACATGGA AACAGTTCCA AATTGTGGAT
    AATGACAACA TCAATGCAGT TCGTTTGGTC ATTGTAGATG GATTGCAGCA TGGACGTATT
    ACTCTGAGAG GTGGGAAAGG ATTTATATTC ACAGTTAAGG ACATCCAGGC TGGAGATGTT
    TGCTACCATC ATGATGACAG TGACACTACT AAGGATTTTA TAGTCTTCAG AATATTTGAT
    GGACGTCACA GCATACGACA CAAGTTTCCT ATTAACATCC TACCAAAAGA TGATAGCCCT
    CCCTTCCTCA TCACAAACAT TGTTATTGAG CTTAGTGAAG GTCAAGCTGT CCTTATCGAG
    GGATCTATGC TTTGTGCTTC TGATATGGAC TCTAGTGATG CATACATACG TTTTAATATC
    ACTAAGCCTT CTGTAGCTGG AGAGATTATA AAGAAGCCAG GACAAAATCT TATTGCTTAT
    CCTGTCACAA GCTTCCTGCA ACGAGACTTA TATAACGGTT TCATTTATTA TCGCCATCTT
    GGAGGAGAAG TATTTGAAGA TTCATTTGAT GTTGTTCTGT CTGATAGCCA TGATCCACCA
    AACTTTTCTG GTCCTCAGGC AGTAATCATT CATATTAGCC CCGTGGATGA CCAGCTGCCC
    ATAGAAGCAC CTGGAACAGT ACGACATCTG ATGGTAAAGG AGAATGAGGT TGTTTATATA
    ACAAAGAAAC AACTGCATTT CATAGACCCA GAGTCACCTG AAAGAGAACT TATTTACACA
    GTTGCTACTC CTCCTTGTAT GGTGCCAACC TACAGCAGCT TAGATGCGGG GAAGATAGTT
    CTTGTAGACA CTATACTTAA ACTTGGAAAG GATTATGCAG GAGACACAGC AAGAACATTT
    TCACAGCATG CTGTGAATCA CCTCAAAGTT GCTTACATAC CACCAGCTAA AGACATTGGG
    CCAACTCCTC TACATGTCCA GTTTATATTA TCAGTGAGTA ACCAACATGG AGGAACAGTT
    CAAGGCATCT GTTTCAATAT CACTATCCTT CCAATAGACA ATCAGCCTCC TGAGGTGTTT
    TCATATGATT TGAAAGTTGA AGAAGGTGGT TTCAGCCAAA TTACTGCAAG TAACATTCAC
    ATCACAGATA AGGACACCAG ACAAGAACAA ATTAAAGTTA TTTTACACAA AGCTCCACTG
    CATGGTATCG TTCAACTTGA TGGATCTGCA TTGCAGGAAG GTGACCCATT CACCTGCTTG
    GACCTCATCA CATTAAAAGT TAGGTACCTT CATGATGGTT CAGAGACCCT ACAAGATGAA
    ATCTCATTTA TTGCAACAGA TGGAATCAAT TCATCTGAGT TTATTTTAAA AATACAGGTT
    TCTCCTATTA ATGATGAACC TCCATTTATT AAAGAGAACC TAATTTCCAC CATTATTTGC
    AAAGAAGGGG CAGAGGTAGT CATATCAGCA GACTATATCT CAGCTACTGA TGCAGACAGT
    GATGACATGA AGCTGATATT TATGATCGCT CGCCAACCAC GTTATGGCAT TTTGCTAAAG
    GCTGGATCTA TCATTGATCA ATTCACCCAG AGAGATGTTG TTGAAGGACT GGTACATTAT
    AGACATACAA GTGGCGAAAT TGGCTTGAAG GTCTATGTTG ATATTATAAC ACTGGTGGTT
    TCTGATGCTG AAGCAGATAA ACCGCAGAAA AAGTGCTGCA ACAATGGTCC TCGTCTGCCG
    ATTTTGCCAA ACCATGAGTC TTTTCCTGTA TATGATCTCA ATGTTACAAT TCTGCCAGTG
    GACAACCAAC CCCCATCGGT TATAATAGGT GAAATATTTG TAGTCGATGA GGGCAAATCA
    TCTGCCCTCA GTGTAGATCA TTTAAGTGCC ATGGACCCTG ACACAGAAAG TAACAATCTG
    GAATTCATAC TGGTTTCTCC TCCTCGATAT GGTTACATTG AAAATGTCCT CCCATCTCCT
    GGATTTGAGA AGAGTAATAT GGGTATAAGT ATTGCTGCAT TTCTACTCAA GGACATTAAA
    TCTCTGAACA TCAATTATGT CCAGTCCCGC CATCAAAGAA TTGAACCAAC CACTGATCAG
    TTTACACTGT ATGTCACTGA TGGCAAACTG CGTTCTGTAG AAAAGCCATT TTACATCGTA
    ATCAATCCGA CAAATGATGA GGTTCCGGAA TTTTTAGCCA GAAACATAAC AGTTGTTGAA
    GGACAAATGA AGAAATTGGA TCCCACTGTA ATAAATGTGT CAGACATGGA TGTGCCACAG
    AACCACTTAT ACTTTACTGT TATAGAGCAG CCCCGACATG GACTGATTAT TGAGGAATCG
    TATATCAGTG GGCACATGAA TAGAAGCCAA TTAATAAAAA GGCATCAGGC AAACAGCCTG
    CCTGTGATAC AAGATTTTTC CATGGACCTG CTAAAAAATG GAATGAAGAT GATATACATG
    CATGATGACT CAGAAAACCT TGCTGACAGC TTCACTATCC AGCTATCTGA TGGAAAGCAT
    GAAGTCAGGA GAACTATTTT TGTGAATGTA ATTGCAGTTA ATGACGAAAA GCCTGTGCTA
    ACCAAGCACT GCGATTTGGT GCTAAGCATG GGGGAACAAA CTCTTATATC AAGTGCCATA
    CTGTCAGCAG AAGATATGGA CACACCCAGG ACAAACATTT CCTATGTTAT TGACAGATGT
    CCAGAGCATG GCCAGCTTCA GATGAAAGTC AATAAAAACT GGGTGACCTT GCATACTGGA
    ATGAAATGCA CTCAGGAGGA CATTGATATG AACCTGGTTA GATATGTGCA TACTGGAACA
    ATGGGCTCAA AAAATGAAGA CACGTTTACT TTCCACCTTT GGGATGGCGG AAACCGATCA
    CCTGAGATAT CTGTTCATAT TTATGTCAAG GATATGGAAA AAGGAGATAT TGCAGTTTTT
    GTGAAAATGC TTAATGTGTC CAAGGGAGAG AGAGTTATCA TAACAACAAA TGTCCTTCTC
    GCCACTGATG GGACCGACAG ACCAGAAGAG CTGCTGTATG TGTTATCCTC GCCACCCCAA
    TATGGGCAGA TTGAATATGT TAAGCATCCT GGAATCCCCA TAACAAGTTT CAGTCAGATG
    GATGTGGCTG CTCACACAAT CTGCTACGTC CACGATTTCA ATTCAGCAGC CACTACTGAT
    ATGTTTAGAT TCCTAGTCAG CAATGGGCTG AAAACGAAAC ATGGGATCAT GAACATCGCA
    CTGGAAACAG TCGATCGAAC TCTTCCAACA CTATCCAGGA ACGATGGTCT AAGACAAGTG
    AGAGGGTCTA TGGAGTGTAT TTCCCCGGCC ACCCTACAGG CACTGGACCC TGACACGTCA
    TCTCAGAACA TTACTTTTCA GTTGGTGGGT CTCCCTCAAT ATGGGCAATT ATACCTCAGA
    GGCTTTCCAC TACAGCAAGA TCACTTCACC CAAAGAGATA TTGACAATCT AGACATTGCC
    TATAAGCATG CTGGACAGGA TGCTCAAATA GACAGGTTTA TGTTTACTGT TACAGACAAA
    GCAAATCAAG GATTTATCAT TAATGGGAAG ATTCAGACGG AACCAGTTCC ATTCACAATA
    CAGGTAGATC CTGTGGACAG CACAGCTCCC AGAATTGTCC ATCTTCAATG CCTTACAGAA
    GTTGAGCTTC TGAAAAATGG CCAGTATGGA ATTTATATTA CTTCAAGACA CTTAAAGGCA
    TCTGATCCAG ACACAGATGA TGAAAGAATT GTCTTTCACA TATTAGGAGG GCCTCACTAT
    GGTTATTTAG AGAATGTTAC AACAGGCAGG TTTATAAGAG AAAGATTTAC ACAAAGGGAT
    TTAAACAGCA AATCAGTGCT CTACGTAATA AATCCATCAG TGGATGTTTT TCATGACAAC
    ATAGAGTTTG AAGTTTCAGA CCCCGTTGGA AACAGAGCTT TACCACAAAT TTTAGAGCTG
    AAATGGAGTC AAATTGAATT GCTCCAAGGC ATGTATGAGG TGTGTGAAAC CGAGGCAGTT
    GTGTCTTTGA AGATGACAAG AAAAGGTTAT CTCCTGGAGT CTGCTTTTGT TTCGCTCAAG
    GTGAATGAAA TAAGTGCTGT TCTTGGCAAA GACATTTCCA TGAATCCTTC CAGACTTATA
    CAGTTTGATC CAGGAGTGTC AACCAAGACT TGGAATATAG TAATTATCCA GGATGGATTA
    GAAGAAGATG ACGAGGTCTT TGAAGTAGTT CTGAGTTCTC CTGTGAATGC TGTTCTTGGC
    TCTAAGACAA GAGCTGTAGT GAAAATTGTG GACCCCAGAG GAGGTGGCTG CAGTTCCTCC
    AAAGTCAGCA TGATCAAAAC AACTGGAATG GGCTTACTAA ACTGTACCGT CAAACCCTCA
    CGCAAACCTT GTGCTGTTTT TAGTTTTAAA GAGACTGCAG ATAAGGTCAG GGAAGATGTC
    CAAGAAGATG TCCATACCTT ACAACTTCCG AGGAAGAGAC TAAGACTCAC AGGGAACAGA
    AAAACAGTTA AACCATCATC AGTATTTAGA AACAAAACAG ACATTATTTA TCAATATCAT
    GGGATTGTGT CTCTCCGAGT GGAAGAAGAT AAGCCAGTAT TAAATGAAAC AAAGAAAGCA
    AAAGTACTAG TGAAGAACCG TGGTCAGAGG AAGATAAATG CCCCCTCCCA AAATCCTGAC
    ATTCTTCAAT CAGGCAGAAC CATGTTGGAT TCTCATGTTA CTACTGGTGG TCAGGATAAC
    GCAATATCGT TTCCTAAAAG ATGCACACCA AGTTTAAAAG GACTCCTGCA TTTTGAGGAC
    ACAGTACAGA AACTGTTCCA GTGTGACGGC ATATCGTGGA ACCCCTGGAT CACTACTAAG
    GATTCTAAAG CAGGAAAATG CCCATTTGGC TGGACTTACC ATGATGGCTA CTGCTATATA
    CTGATCAATG ATCAAAAGGC CACCTGGATA ACAGCAGCCA GAGCATGTAG AGAACAGCAT
    TTTGCTACTC TGGTGGATGT ATTCAGCAAG GAACAAATGG AATGGCTTTG GAATTTCAGT
    GGCAGAAAAT CCTTTTGGAT AGGTGACTAC TTATATTATT ATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48723
    Clone ID Related Accession (Same CDS sequence) XM_031890521.1
    Accession Version XM_031890521.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product FRAS1-related extracellular matrix protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGAAGCCTT CTATTGTATA CCCAGTTTTG CTGTCAGTGT TGCTCACAAG GGGCCTCTGC 
    GCCTCCTTTA TCAAAGTCAA TCACGGAATG AAAGTTATGA AAGGACAGAG TGCATTCCTC
    TCAGAAAGTG ACCTGCAGTT TTCCATCCCA AGGGAAAAGG ATGCCTGCAA AGTGGAAGTA
    GTTGTTAATG AACCCATTAC ACAGAGAGTT GGAAGGTTAA CACCACAGGT GTTTGACTGC
    CATTTCCTAG CCAATGAAGT TCAGTACACA CACAATGGCT GCCCTATCTT GGATGAAGAC
    GAGGTCATGC TCAGACTTTA CAGATTTACA GAAACTGAGA CTTTTACTGA AACCTTCCTG
    CTGAGAGTGA AGTTAATAGA GCCTGACTGT AATATCATAC AGCTGGGGCC TAAAAAGCTT
    GAAGTGCCAG AGTTTTATGG TCTGTCTGAC GTTATTGATA AGAACATACT GAGCTTTGAC
    TATGACAAAA GAATAAACAT TGAATGTACC ATCAGAATAT CGTCTTCTGA ATCTTTCCTT
    CCTGCACACG GTCAGCTTGT GACGGGGGAT CCTGTGAAAC AAGAACCACG AGGAGATCAG
    CCACACAGCT TCTTCCATGG ATCAAATCAA AAGCCAGGAC CTTTGTGCAA GAATGGAGGC
    TGCATACAAG GTTTAAAGAG GATCCAGAGC GTTATAAAAA TGAGTTGTGA AGATTTCCTT
    GTAAAGGGCA TCCGATACCA GCACTTGGAT CCTCCATCTC CAAATGTTGA TTATATCAGT
    ATCAGGTTGG ATCTTACAGA CTCAAGGAGC AAATCACTTT ACAAGTCAGA ACATGCTTGG
    ATTCCTGTTC TCATTCGGGA TGCTGTTCCA AATCAAATCC CCAAAGCCAA CTTCATGTCT
    ATGTTTATCT TAGAAGTAGA TCAGTTTGTT CTAACACCCA TCTCAACTGC AACACTGGAT
    GCCGAGGACA GCGAAACAGC CAAACATCTT CTGGTCTTCA ATATTACTTT ACCTCCAAGC
    AAAGGGTTCA TCACACACTT GTCTGACCAT ACCAAGCCCA TTACATCCTT TACATGGAAA
    GATCTCAATG AAATGCTCAT AGCATATCAG CCTCCGAACA GCAGCCACAC AGAGAGAAGA
    AATTTGGAGG TGGAGTTTGA GGTGCATGAT TTCTTTTTTG AAAAGAGTCC TCCAATTACA
    GTGCATATCT CTATACGTAC GGCTGACACC AATGCTCCTC GCGTTTCATG GAACATGGGT
    CTGGATTTAC TGGAGGGCCA GTCTCGACCA ATTACATGGA AACAGTTCCA AATTGTGGAT
    AATGACAACA TCAATGCAGT TCGTTTGGTC ATTGTAGATG GATTGCAGCA TGGACGTATT
    ACTCTGAGAG GTGGGAAAGG ATTTATATTC ACAGTTAAGG ACATCCAGGC TGGAGATGTT
    TGCTACCATC ATGATGACAG TGACACTACT AAGGATTTTA TAGTCTTCAG AATATTTGAT
    GGACGTCACA GCATACGACA CAAGTTTCCT ATTAACATCC TACCAAAAGA TGATAGCCCT
    CCCTTCCTCA TCACAAACAT TGTTATTGAG CTTAGTGAAG GTCAAGCTGT CCTTATCGAG
    GGATCTATGC TTTGTGCTTC TGATATGGAC TCTAGTGATG CATACATACG TTTTAATATC
    ACTAAGCCTT CTGTAGCTGG AGAGATTATA AAGAAGCCAG GACAAAATCT TATTGCTTAT
    CCTGTCACAA GCTTCCTGCA ACGAGACTTA TATAACGGTT TCATTTATTA TCGCCATCTT
    GGAGGAGAAG TATTTGAAGA TTCATTTGAT GTTGTTCTGT CTGATAGCCA TGATCCACCA
    AACTTTTCTG GTCCTCAGGC AGTAATCATT CATATTAGCC CCGTGGATGA CCAGCTGCCC
    ATAGAAGCAC CTGGAACAGT ACGACATCTG ATGGTAAAGG AGAATGAGGT TGTTTATATA
    ACAAAGAAAC AACTGCATTT CATAGACCCA GAGTCACCTG AAAGAGAACT TATTTACACA
    GTTGCTACTC CTCCTTGTAT GGTGCCAACC TACAGCTTAG ATGCGGGGAA GATAGTTCTT
    GTAGACACTA TACTTAAACT TGGAAAGGAT TATGCAGGAG ACACAGCAAG AACATTTTCA
    CAGCATGCTG TGAATCACCT CAAAGTTGCT TACATACCAC CAGCTAAAGA CATTGGGCCA
    ACTCCTCTAC ATGTCCAGTT TATATTATCA GTGAGTAACC AACATGGAGG AACAGTTCAA
    GGCATCTGTT TCAATATCAC TATCCTTCCA ATAGACAATC AGCCTCCTGA GGTGTTTTCA
    TATGATTTGA AAGTTGAAGA AGGTGGTTTC AGCCAAATTA CTGCAAGTAA CATTCACATC
    ACAGATAAGG ACACCAGACA AGAACAAATT AAAGTTATTT TACACAAAGC TCCACTGCAT
    GGTATCGTTC AACTTGATGG ATCTGCATTG CAGGAAGGTG ACCCATTCAC CTGCTTGGAC
    CTCATCACAT TAAAAGTTAG GTACCTTCAT GATGGTTCAG AGACCCTACA AGATGAAATC
    TCATTTATTG CAACAGATGG AATCAATTCA TCTGAGTTTA TTTTAAAAAT ACAGGTTTCT
    CCTATTAATG ATGAACCTCC ATTTATTAAA GAGAACCTAA TTTCCACCAT TATTTGCAAA
    GAAGGGGCAG AGGTAGTCAT ATCAGCAGAC TATATCTCAG CTACTGATGC AGACAGTGAT
    GACATGAAGC TGATATTTAT GATCGCTCGC CAACCACGTT ATGGCATTTT GCTAAAGGCT
    GGATCTATCA TTGATCAATT CACCCAGAGA GATGTTGTTG AAGGACTGGT ACATTATAGA
    CATACAAGTG GCGAAATTGG CTTGAAGGTC TATGTTGATA TTATAACACT GGTGGTTTCT
    GATGCTGAAG CAGATAAACC GCAGAAAAAG TGCTGCAACA ATGGTCCTCG TCTGCCGATT
    TTGCCAAACC ATGAGTCTTT TCCTGTATAT GATCTCAATG TTACAATTCT GCCAGTGGAC
    AACCAACCCC CATCGGTTAT AATAGGTGAA ATATTTGTAG TCGATGAGGG CAAATCATCT
    GCCCTCAGTG TAGATCATTT AAGTGCCATG GACCCTGACA CAGAAAGTAA CAATCTGGAA
    TTCATACTGG TTTCTCCTCC TCGATATGGT TACATTGAAA ATGTCCTCCC ATCTCCTGGA
    TTTGAGAAGA GTAATATGGG TATAAGTATT GCTGCATTTC TACTCAAGGA CATTAAATCT
    CTGAACATCA ATTATGTCCA GTCCCGCCAT CAAAGAATTG AACCAACCAC TGATCAGTTT
    ACACTGTATG TCACTGATGG CAAACTGCGT TCTGTAGAAA AGCCATTTTA CATCGTAATC
    AATCCGACAA ATGATGAGGT TCCGGAATTT TTAGCCAGAA ACATAACAGT TGTTGAAGGA
    CAAATGAAGA AATTGGATCC CACTGTAATA AATGTGTCAG ACATGGATGT GCCACAGAAC
    CACTTATACT TTACTGTTAT AGAGCAGCCC CGACATGGAC TGATTATTGA GGAATCGTAT
    ATCAGTGGGC ACATGAATAG AAGCCAATTA ATAAAAAGGC ATCAGGCAAA CAGCCTGCCT
    GTGATACAAG ATTTTTCCAT GGACCTGCTA AAAAATGGAA TGAAGATGAT ATACATGCAT
    GATGACTCAG AAAACCTTGC TGACAGCTTC ACTATCCAGC TATCTGATGG AAAGCATGAA
    GTCAGGAGAA CTATTTTTGT GAATGTAATT GCAGTTAATG ACGAAAAGCC TGTGCTAACC
    AAGCACTGCG ATTTGGTGCT AAGCATGGGG GAACAAACTC TTATATCAAG TGCCATACTG
    TCAGCAGAAG ATATGGACAC ACCCAGGACA AACATTTCCT ATGTTATTGA CAGATGTCCA
    GAGCATGGCC AGCTTCAGAT GAAAGTCAAT AAAAACTGGG TGACCTTGCA TACTGGAATG
    AAATGCACTC AGGAGGACAT TGATATGAAC CTGGTTAGAT ATGTGCATAC TGGAACAATG
    GGCTCAAAAA ATGAAGACAC GTTTACTTTC CACCTTTGGG ATGGCGGAAA CCGATCACCT
    GAGATATCTG TTCATATTTA TGTCAAGGAT ATGGAAAAAG GAGATATTGC AGTTTTTGTG
    AAAATGCTTA ATGTGTCCAA GGGAGAGAGA GTTATCATAA CAACAAATGT CCTTCTCGCC
    ACTGATGGGA CCGACAGACC AGAAGAGCTG CTGTATGTGT TATCCTCGCC ACCCCAATAT
    GGGCAGATTG AATATGTTAA GCATCCTGGA ATCCCCATAA CAAGTTTCAG TCAGATGGAT
    GTGGCTGCTC ACACAATCTG CTACGTCCAC GATTTCAATT CAGCAGCCAC TACTGATATG
    TTTAGATTCC TAGTCAGCAA TGGGCTGAAA ACGAAACATG GGATCATGAA CATCGCACTG
    GAAACAGTCG ATCGAACTCT TCCAACACTA TCCAGGAACG ATGGTCTAAG ACAAGTGAGA
    GGGTCTATGG AGTGTATTTC CCCGGCCACC CTACAGGCAC TGGACCCTGA CACGTCATCT
    CAGAACATTA CTTTTCAGTT GGTGGGTCTC CCTCAATATG GGCAATTATA CCTCAGAGGC
    TTTCCACTAC AGCAAGATCA CTTCACCCAA AGAGATATTG ACAATCTAGA CATTGCCTAT
    AAGCATGCTG GACAGGATGC TCAAATAGAC AGGTTTATGT TTACTGTTAC AGACAAAGCA
    AATCAAGGAT TTATCATTAA TGGGAAGATT CAGACGGAAC CAGTTCCATT CACAATACAG
    GTAGATCCTG TGGACAGCAC AGCTCCCAGA ATTGTCCATC TTCAATGCCT TACAGAAGTT
    GAGCTTCTGA AAAATGGCCA GTATGGAATT TATATTACTT CAAGACACTT AAAGGCATCT
    GATCCAGACA CAGATGATGA AAGAATTGTC TTTCACATAT TAGGAGGGCC TCACTATGGT
    TATTTAGAGA ATGTTACAAC AGGCAGGTTT ATAAGAGAAA GATTTACACA AAGGGATTTA
    AACAGCAAAT CAGTGCTCTA CGTAATAAAT CCATCAGTGG ATGTTTTTCA TGACAACATA
    GAGTTTGAAG TTTCAGACCC CGTTGGAAAC AGAGCTTTAC CACAAATTTT AGAGCTGAAA
    TGGAGTCAAA TTGAATTGCT CCAAGGCATG TATGAGGTGT GTGAAACCGA GGCAGTTGTG
    TCTTTGAAGA TGACAAGAAA AGGTTATCTC CTGGAGTCTG CTTTTGTTTC GCTCAAGGTG
    AATGAAATAA GTGCTGTTCT TGGCAAAGAC ATTTCCATGA ATCCTTCCAG ACTTATACAG
    TTTGATCCAG GAGTGTCAAC CAAGACTTGG AATATAGTAA TTATCCAGGA TGGATTAGAA
    GAAGATGACG AGGTCTTTGA AGTAGTTCTG AGTTCTCCTG TGAATGCTGT TCTTGGCTCT
    AAGACAAGAG CTGTAGTGAA AATTGTGGAC CCCAGAGGAG GTGGCTGCAG TTCCTCCAAA
    GTCAGCATGA TCAAAACAAC TGGAATGGGC TTACTAAACT GTACCGTCAA ACCCTCACGC
    AAACCTTGTG CTGTTTTTAG TTTTAAAGAG ACTGCAGATA AGGTCAGGGA AGATGTCCAA
    GAAGATGTCC ATACCTTACA ACTTCCGAGG AAGAGACTAA GACTCACAGG GAACAGAAAA
    ACAGTTAAAC CATCATCAGT ATTTAGAAAC AAAACAGACA TTATTTATCA ATATCATGGG
    ATTGTGTCTC TCCGAGTGGA AGAAGATAAG CCAGTATTAA ATGAAACAAA GAAAGCAAAA
    GTACTAGTGA AGAACCGTGG TCAGAGGAAG ATAAATGCCC CCTCCCAAAA TCCTGACATT
    CTTCAATCAG GCAGAACCAT GTTGGATTCT CATGTTACTA CTGGTGGTCA GGATAACGCA
    ATATCGTTTC CTAAAAGATG CACACCAAGT TTAAAAGGAC TCCTGCATTT TGAGGACACA
    GTACAGAAAC TGTTCCAGTG TGACGGCATA TCGTGGAACC CCTGGATCAC TACTAAGGAT
    TCTAAAGCAG GAAAATGCCC ATTTGGCTGG ACTTACCATG ATGGCTACTG CTATATACTG
    ATCAATGATC AAAAGGCCAC CTGGATAACA GCAGCCAGAG CATGTAGAGA ACAGCATTTT
    GCTACTCTGG TGGATGTATT CAGCAAGGAA CAAATGGAAT GGCTTTGGAA TTTCAGTGGC
    AGAAAATCCT TTTGGATAGG CCTCAATGAT AGAGTACGTA ATGGAAACTG GGAGTGGAGC
    AAAGGGGAAA TGGTCACCTT CACCAATTGG AAAAGAGGCT CTCCTCGATC TTCACACAAA
    GGGAAGAATT GCGTTTTGGC ACAAAAGAGA GGCAAATGGC AGGCTAAAGA CTGCAAAAAA
    GGCAAAGGCC ATTACTATAT ATGTTCAAAA AAGTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031746381.1
    CDS613..7131
    Translation

    Target ORF information:

    RefSeq Version XM_031890521.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis FRAS1 related extracellular matrix 1 (frem1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031890521.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGAAGCCTT CTATTGTATA CCCAGTTTTG CTGTCAGTGT TGCTCACAAG GGGCCTCTGC 
    GCCTCCTTTA TCAAAGTCAA TCACGGAATG AAAGTTATGA AAGGACAGAG TGCATTCCTC
    TCAGAAAGTG ACCTGCAGTT TTCCATCCCA AGGGAAAAGG ATGCCTGCAA AGTGGAAGTA
    GTTGTTAATG AACCCATTAC ACAGAGAGTT GGAAGGTTAA CACCACAGGT GTTTGACTGC
    CATTTCCTAG CCAATGAAGT TCAGTACACA CACAATGGCT GCCCTATCTT GGATGAAGAC
    GAGGTCATGC TCAGACTTTA CAGATTTACA GAAACTGAGA CTTTTACTGA AACCTTCCTG
    CTGAGAGTGA AGTTAATAGA GCCTGACTGT AATATCATAC AGCTGGGGCC TAAAAAGCTT
    GAAGTGCCAG AGTTTTATGG TCTGTCTGAC GTTATTGATA AGAACATACT GAGCTTTGAC
    TATGACAAAA GAATAAACAT TGAATGTACC ATCAGAATAT CGTCTTCTGA ATCTTTCCTT
    CCTGCACACG GTCAGCTTGT GACGGGGGAT CCTGTGAAAC AAGAACCACG AGGAGATCAG
    CCACACAGCT TCTTCCATGG ATCAAATCAA AAGCCAGGAC CTTTGTGCAA GAATGGAGGC
    TGCATACAAG GTTTAAAGAG GATCCAGAGC GTTATAAAAA TGAGTTGTGA AGATTTCCTT
    GTAAAGGGCA TCCGATACCA GCACTTGGAT CCTCCATCTC CAAATGTTGA TTATATCAGT
    ATCAGGTTGG ATCTTACAGA CTCAAGGAGC AAATCACTTT ACAAGTCAGA ACATGCTTGG
    ATTCCTGTTC TCATTCGGGA TGCTGTTCCA AATCAAATCC CCAAAGCCAA CTTCATGTCT
    ATGTTTATCT TAGAAGTAGA TCAGTTTGTT CTAACACCCA TCTCAACTGC AACACTGGAT
    GCCGAGGACA GCGAAACAGC CAAACATCTT CTGGTCTTCA ATATTACTTT ACCTCCAAGC
    AAAGGGTTCA TCACACACTT GTCTGACCAT ACCAAGCCCA TTACATCCTT TACATGGAAA
    GATCTCAATG AAATGCTCAT AGCATATCAG CCTCCGAACA GCAGCCACAC AGAGAGAAGA
    AATTTGGAGG TGGAGTTTGA GGTGCATGAT TTCTTTTTTG AAAAGAGTCC TCCAATTACA
    GTGCATATCT CTATACGTAC GGCTGACACC AATGCTCCTC GCGTTTCATG GAACATGGGT
    CTGGATTTAC TGGAGGGCCA GTCTCGACCA ATTACATGGA AACAGTTCCA AATTGTGGAT
    AATGACAACA TCAATGCAGT TCGTTTGGTC ATTGTAGATG GATTGCAGCA TGGACGTATT
    ACTCTGAGAG GTGGGAAAGG ATTTATATTC ACAGTTAAGG ACATCCAGGC TGGAGATGTT
    TGCTACCATC ATGATGACAG TGACACTACT AAGGATTTTA TAGTCTTCAG AATATTTGAT
    GGACGTCACA GCATACGACA CAAGTTTCCT ATTAACATCC TACCAAAAGA TGATAGCCCT
    CCCTTCCTCA TCACAAACAT TGTTATTGAG CTTAGTGAAG GTCAAGCTGT CCTTATCGAG
    GGATCTATGC TTTGTGCTTC TGATATGGAC TCTAGTGATG CATACATACG TTTTAATATC
    ACTAAGCCTT CTGTAGCTGG AGAGATTATA AAGAAGCCAG GACAAAATCT TATTGCTTAT
    CCTGTCACAA GCTTCCTGCA ACGAGACTTA TATAACGGTT TCATTTATTA TCGCCATCTT
    GGAGGAGAAG TATTTGAAGA TTCATTTGAT GTTGTTCTGT CTGATAGCCA TGATCCACCA
    AACTTTTCTG GTCCTCAGGC AGTAATCATT CATATTAGCC CCGTGGATGA CCAGCTGCCC
    ATAGAAGCAC CTGGAACAGT ACGACATCTG ATGGTAAAGG AGAATGAGGT TGTTTATATA
    ACAAAGAAAC AACTGCATTT CATAGACCCA GAGTCACCTG AAAGAGAACT TATTTACACA
    GTTGCTACTC CTCCTTGTAT GGTGCCAACC TACAGCTTAG ATGCGGGGAA GATAGTTCTT
    GTAGACACTA TACTTAAACT TGGAAAGGAT TATGCAGGAG ACACAGCAAG AACATTTTCA
    CAGCATGCTG TGAATCACCT CAAAGTTGCT TACATACCAC CAGCTAAAGA CATTGGGCCA
    ACTCCTCTAC ATGTCCAGTT TATATTATCA GTGAGTAACC AACATGGAGG AACAGTTCAA
    GGCATCTGTT TCAATATCAC TATCCTTCCA ATAGACAATC AGCCTCCTGA GGTGTTTTCA
    TATGATTTGA AAGTTGAAGA AGGTGGTTTC AGCCAAATTA CTGCAAGTAA CATTCACATC
    ACAGATAAGG ACACCAGACA AGAACAAATT AAAGTTATTT TACACAAAGC TCCACTGCAT
    GGTATCGTTC AACTTGATGG ATCTGCATTG CAGGAAGGTG ACCCATTCAC CTGCTTGGAC
    CTCATCACAT TAAAAGTTAG GTACCTTCAT GATGGTTCAG AGACCCTACA AGATGAAATC
    TCATTTATTG CAACAGATGG AATCAATTCA TCTGAGTTTA TTTTAAAAAT ACAGGTTTCT
    CCTATTAATG ATGAACCTCC ATTTATTAAA GAGAACCTAA TTTCCACCAT TATTTGCAAA
    GAAGGGGCAG AGGTAGTCAT ATCAGCAGAC TATATCTCAG CTACTGATGC AGACAGTGAT
    GACATGAAGC TGATATTTAT GATCGCTCGC CAACCACGTT ATGGCATTTT GCTAAAGGCT
    GGATCTATCA TTGATCAATT CACCCAGAGA GATGTTGTTG AAGGACTGGT ACATTATAGA
    CATACAAGTG GCGAAATTGG CTTGAAGGTC TATGTTGATA TTATAACACT GGTGGTTTCT
    GATGCTGAAG CAGATAAACC GCAGAAAAAG TGCTGCAACA ATGGTCCTCG TCTGCCGATT
    TTGCCAAACC ATGAGTCTTT TCCTGTATAT GATCTCAATG TTACAATTCT GCCAGTGGAC
    AACCAACCCC CATCGGTTAT AATAGGTGAA ATATTTGTAG TCGATGAGGG CAAATCATCT
    GCCCTCAGTG TAGATCATTT AAGTGCCATG GACCCTGACA CAGAAAGTAA CAATCTGGAA
    TTCATACTGG TTTCTCCTCC TCGATATGGT TACATTGAAA ATGTCCTCCC ATCTCCTGGA
    TTTGAGAAGA GTAATATGGG TATAAGTATT GCTGCATTTC TACTCAAGGA CATTAAATCT
    CTGAACATCA ATTATGTCCA GTCCCGCCAT CAAAGAATTG AACCAACCAC TGATCAGTTT
    ACACTGTATG TCACTGATGG CAAACTGCGT TCTGTAGAAA AGCCATTTTA CATCGTAATC
    AATCCGACAA ATGATGAGGT TCCGGAATTT TTAGCCAGAA ACATAACAGT TGTTGAAGGA
    CAAATGAAGA AATTGGATCC CACTGTAATA AATGTGTCAG ACATGGATGT GCCACAGAAC
    CACTTATACT TTACTGTTAT AGAGCAGCCC CGACATGGAC TGATTATTGA GGAATCGTAT
    ATCAGTGGGC ACATGAATAG AAGCCAATTA ATAAAAAGGC ATCAGGCAAA CAGCCTGCCT
    GTGATACAAG ATTTTTCCAT GGACCTGCTA AAAAATGGAA TGAAGATGAT ATACATGCAT
    GATGACTCAG AAAACCTTGC TGACAGCTTC ACTATCCAGC TATCTGATGG AAAGCATGAA
    GTCAGGAGAA CTATTTTTGT GAATGTAATT GCAGTTAATG ACGAAAAGCC TGTGCTAACC
    AAGCACTGCG ATTTGGTGCT AAGCATGGGG GAACAAACTC TTATATCAAG TGCCATACTG
    TCAGCAGAAG ATATGGACAC ACCCAGGACA AACATTTCCT ATGTTATTGA CAGATGTCCA
    GAGCATGGCC AGCTTCAGAT GAAAGTCAAT AAAAACTGGG TGACCTTGCA TACTGGAATG
    AAATGCACTC AGGAGGACAT TGATATGAAC CTGGTTAGAT ATGTGCATAC TGGAACAATG
    GGCTCAAAAA ATGAAGACAC GTTTACTTTC CACCTTTGGG ATGGCGGAAA CCGATCACCT
    GAGATATCTG TTCATATTTA TGTCAAGGAT ATGGAAAAAG GAGATATTGC AGTTTTTGTG
    AAAATGCTTA ATGTGTCCAA GGGAGAGAGA GTTATCATAA CAACAAATGT CCTTCTCGCC
    ACTGATGGGA CCGACAGACC AGAAGAGCTG CTGTATGTGT TATCCTCGCC ACCCCAATAT
    GGGCAGATTG AATATGTTAA GCATCCTGGA ATCCCCATAA CAAGTTTCAG TCAGATGGAT
    GTGGCTGCTC ACACAATCTG CTACGTCCAC GATTTCAATT CAGCAGCCAC TACTGATATG
    TTTAGATTCC TAGTCAGCAA TGGGCTGAAA ACGAAACATG GGATCATGAA CATCGCACTG
    GAAACAGTCG ATCGAACTCT TCCAACACTA TCCAGGAACG ATGGTCTAAG ACAAGTGAGA
    GGGTCTATGG AGTGTATTTC CCCGGCCACC CTACAGGCAC TGGACCCTGA CACGTCATCT
    CAGAACATTA CTTTTCAGTT GGTGGGTCTC CCTCAATATG GGCAATTATA CCTCAGAGGC
    TTTCCACTAC AGCAAGATCA CTTCACCCAA AGAGATATTG ACAATCTAGA CATTGCCTAT
    AAGCATGCTG GACAGGATGC TCAAATAGAC AGGTTTATGT TTACTGTTAC AGACAAAGCA
    AATCAAGGAT TTATCATTAA TGGGAAGATT CAGACGGAAC CAGTTCCATT CACAATACAG
    GTAGATCCTG TGGACAGCAC AGCTCCCAGA ATTGTCCATC TTCAATGCCT TACAGAAGTT
    GAGCTTCTGA AAAATGGCCA GTATGGAATT TATATTACTT CAAGACACTT AAAGGCATCT
    GATCCAGACA CAGATGATGA AAGAATTGTC TTTCACATAT TAGGAGGGCC TCACTATGGT
    TATTTAGAGA ATGTTACAAC AGGCAGGTTT ATAAGAGAAA GATTTACACA AAGGGATTTA
    AACAGCAAAT CAGTGCTCTA CGTAATAAAT CCATCAGTGG ATGTTTTTCA TGACAACATA
    GAGTTTGAAG TTTCAGACCC CGTTGGAAAC AGAGCTTTAC CACAAATTTT AGAGCTGAAA
    TGGAGTCAAA TTGAATTGCT CCAAGGCATG TATGAGGTGT GTGAAACCGA GGCAGTTGTG
    TCTTTGAAGA TGACAAGAAA AGGTTATCTC CTGGAGTCTG CTTTTGTTTC GCTCAAGGTG
    AATGAAATAA GTGCTGTTCT TGGCAAAGAC ATTTCCATGA ATCCTTCCAG ACTTATACAG
    TTTGATCCAG GAGTGTCAAC CAAGACTTGG AATATAGTAA TTATCCAGGA TGGATTAGAA
    GAAGATGACG AGGTCTTTGA AGTAGTTCTG AGTTCTCCTG TGAATGCTGT TCTTGGCTCT
    AAGACAAGAG CTGTAGTGAA AATTGTGGAC CCCAGAGGAG GTGGCTGCAG TTCCTCCAAA
    GTCAGCATGA TCAAAACAAC TGGAATGGGC TTACTAAACT GTACCGTCAA ACCCTCACGC
    AAACCTTGTG CTGTTTTTAG TTTTAAAGAG ACTGCAGATA AGGTCAGGGA AGATGTCCAA
    GAAGATGTCC ATACCTTACA ACTTCCGAGG AAGAGACTAA GACTCACAGG GAACAGAAAA
    ACAGTTAAAC CATCATCAGT ATTTAGAAAC AAAACAGACA TTATTTATCA ATATCATGGG
    ATTGTGTCTC TCCGAGTGGA AGAAGATAAG CCAGTATTAA ATGAAACAAA GAAAGCAAAA
    GTACTAGTGA AGAACCGTGG TCAGAGGAAG ATAAATGCCC CCTCCCAAAA TCCTGACATT
    CTTCAATCAG GCAGAACCAT GTTGGATTCT CATGTTACTA CTGGTGGTCA GGATAACGCA
    ATATCGTTTC CTAAAAGATG CACACCAAGT TTAAAAGGAC TCCTGCATTT TGAGGACACA
    GTACAGAAAC TGTTCCAGTG TGACGGCATA TCGTGGAACC CCTGGATCAC TACTAAGGAT
    TCTAAAGCAG GAAAATGCCC ATTTGGCTGG ACTTACCATG ATGGCTACTG CTATATACTG
    ATCAATGATC AAAAGGCCAC CTGGATAACA GCAGCCAGAG CATGTAGAGA ACAGCATTTT
    GCTACTCTGG TGGATGTATT CAGCAAGGAA CAAATGGAAT GGCTTTGGAA TTTCAGTGGC
    AGAAAATCCT TTTGGATAGG CCTCAATGAT AGAGTACGTA ATGGAAACTG GGAGTGGAGC
    AAAGGGGAAA TGGTCACCTT CACCAATTGG AAAAGAGGCT CTCCTCGATC TTCACACAAA
    GGGAAGAATT GCGTTTTGGC ACAAAAGAGA GGCAAATGGC AGGCTAAAGA CTGCAAAAAA
    GGCAAAGGCC ATTACTATAT ATGTTCAAAA AAGTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa04017
    Clone ID Related Accession (Same CDS sequence) XM_002939090.4 , XM_002939090.5
    Accession Version XM_002939090.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6522bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product FRAS1-related extracellular matrix protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002939090.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGAAGCCTT CTATTGTATA CCCAGTTTTG CTGTCAGTGT TGCTCACAAG GGGCCTCTGC 
    GCCTCCTTTA TCAAAGTCAA TCACGGAATG AAAGTTATGA AAGGACAGAG TGCATTCCTC
    TCAGAAAGTG ACCTGCAGTT TTCCATCCCA AGGGAAAAGG ATGCCTGCAA AGTGGAAGTA
    GTTGTTAATG AACCCATTAC ACAGAGAGTT GGAAGGTTAA CACCACAGGT GTTTGACTGC
    CATTTCCTAG CCAATGAAGT TCAGTACACA CACAATGGCT GCCCTATCTT GGATGAAGAC
    GAGGTCATGC TCAGACTTTA CAGATTTACA GAAACTGAGA CTTTTACTGA AACCTTCCTG
    CTGAGAGTGA AGTTAATAGA GCCTGACTGT AATATCATAC AGCTGGGGCC TAAAAAGCTT
    GAAGTGCCAG AGTTTTATGG TCTGTCTGAC GTTATTGATA AGAACATACT GAGCTTTGAC
    TATGACAAAA GAATAAACAT TGAATGTACC ATCAGAATAT CGTCTTCTGA ATCTTTCCTT
    CCTGCACACG GTCAGCTTGT GACGGGGGAT CCTGTGAAAC AAGAACCACG AGGAGATCAG
    CCACACAGCT TCTTCCATGG ATCAAATCAA AAGCCAGGAC CTTTGTGCAA GAATGGAGGC
    TGCATACAAG GTTTAAAGAG GATCCAGAGC GTTATAAAAA TGAGTTGTGA AGATTTCCTT
    GTAAAGGGCA TCCGATACCA GCACTTGGAT CCTCCATCTC CAAATGTTGA TTATATCAGT
    ATCAGGTTGG ATCTTACAGA CTCAAGGAGC AAATCACTTT ACAAGTCAGA ACATGCTTGG
    ATTCCTGTTC TCATTCGGGA TGCTGTTCCA AATCAAATCC CCAAAGCCAA CTTCATGTCT
    ATGTTTATCT TAGAAGTAGA TCAGTTTGTT CTAACACCCA TCTCAACTGC AACACTGGAT
    GCCGAGGACA GCGAAACAGC CAAACATCTT CTGGTCTTCA ATATTACTTT ACCTCCAAGC
    AAAGGGTTCA TCACACACTT GTCTGACCAT ACCAAGCCCA TTACATCCTT TACATGGAAA
    GATCTCAATG AAATGCTCAT AGCATATCAG CCTCCGAACA GCAGCCACAC AGAGAGAAGA
    AATTTGGAGG TGGAGTTTGA GGTGCATGAT TTCTTTTTTG AAAAGAGTCC TCCAATTACA
    GTGCATATCT CTATACGTAC GGCTGACACC AATGCTCCTC GCGTTTCATG GAACATGGGT
    CTGGATTTAC TGGAGGGCCA GTCTCGACCA ATTACATGGA AACAGTTCCA AATTGTGGAT
    AATGACAACA TCAATGCAGT TCGTTTGGTC ATTGTAGATG GATTGCAGCA TGGACGTATT
    ACTCTGAGAG GTGGGAAAGG ATTTATATTC ACAGTTAAGG ACATCCAGGC TGGAGATGTT
    TGCTACCATC ATGATGACAG TGACACTACT AAGGATTTTA TAGTCTTCAG AATATTTGAT
    GGACGTCACA GCATACGACA CAAGTTTCCT ATTAACATCC TACCAAAAGA TGATAGCCCT
    CCCTTCCTCA TCACAAACAT TGTTATTGAG CTTAGTGAAG GTCAAGCTGT CCTTATCGAG
    GGATCTATGC TTTGTGCTTC TGATATGGAC TCTAGTGATG CATACATACG TTTTAATATC
    ACTAAGCCTT CTGTAGCTGG AGAGATTATA AAGAAGCCAG GACAAAATCT TATTGCTTAT
    CCTGTCACAA GCTTCCTGCA ACGAGACTTA TATAACGGTT TCATTTATTA TCGCCATCTT
    GGAGGAGAAG TATTTGAAGA TTCATTTGAT GTTGTTCTGT CTGATAGCCA TGATCCACCA
    AACTTTTCTG GTCCTCAGGC AGTAATCATT CATATTAGCC CCGTGGATGA CCAGCTGCCC
    ATAGAAGCAC CTGGAACAGT ACGACATCTG ATGGTAAAGG AGAATGAGGT TGTTTATATA
    ACAAAGAAAC AACTGCATTT CATAGACCCA GAGTCACCTG AAAGAGAACT TATTTACACA
    GTTGCTACTC CTCCTTGTAT GGTGCCAACC TACAGCAGCT TAGATGCGGG GAAGATAGTT
    CTTGTAGACA CTATACTTAA ACTTGGAAAG GATTATGCAG GAGACACAGC AAGAACATTT
    TCACAGCATG CTGTGAATCA CCTCAAAGTT GCTTACATAC CACCAGCTAA AGACATTGGG
    CCAACTCCTC TACATGTCCA GTTTATATTA TCAGTGAGTA ACCAACATGG AGGAACAGTT
    CAAGGCATCT GTTTCAATAT CACTATCCTT CCAATAGACA ATCAGCCTCC TGAGGTGTTT
    TCATATGATT TGAAAGTTGA AGAAGGTGGT TTCAGCCAAA TTACTGCAAG TAACATTCAC
    ATCACAGATA AGGACACCAG ACAAGAACAA ATTAAAGTTA TTTTACACAA AGCTCCACTG
    CATGGTATCG TTCAACTTGA TGGATCTGCA TTGCAGGAAG GTGACCCATT CACCTGCTTG
    GACCTCATCA CATTAAAAGT TAGGTACCTT CATGATGGTT CAGAGACCCT ACAAGATGAA
    ATCTCATTTA TTGCAACAGA TGGAATCAAT TCATCTGAGT TTATTTTAAA AATACAGGTT
    TCTCCTATTA ATGATGAACC TCCATTTATT AAAGAGAACC TAATTTCCAC CATTATTTGC
    AAAGAAGGGG CAGAGGTAGT CATATCAGCA GACTATATCT CAGCTACTGA TGCAGACAGT
    GATGACATGA AGCTGATATT TATGATCGCT CGCCAACCAC GTTATGGCAT TTTGCTAAAG
    GCTGGATCTA TCATTGATCA ATTCACCCAG AGAGATGTTG TTGAAGGACT GGTACATTAT
    AGACATACAA GTGGCGAAAT TGGCTTGAAG GTCTATGTTG ATATTATAAC ACTGGTGGTT
    TCTGATGCTG AAGCAGATAA ACCGCAGAAA AAGTGCTGCA ACAATGGTCC TCGTCTGCCG
    ATTTTGCCAA ACCATGAGTC TTTTCCTGTA TATGATCTCA ATGTTACAAT TCTGCCAGTG
    GACAACCAAC CCCCATCGGT TATAATAGGT GAAATATTTG TAGTCGATGA GGGCAAATCA
    TCTGCCCTCA GTGTAGATCA TTTAAGTGCC ATGGACCCTG ACACAGAAAG TAACAATCTG
    GAATTCATAC TGGTTTCTCC TCCTCGATAT GGTTACATTG AAAATGTCCT CCCATCTCCT
    GGATTTGAGA AGAGTAATAT GGGTATAAGT ATTGCTGCAT TTCTACTCAA GGACATTAAA
    TCTCTGAACA TCAATTATGT CCAGTCCCGC CATCAAAGAA TTGAACCAAC CACTGATCAG
    TTTACACTGT ATGTCACTGA TGGCAAACTG CGTTCTGTAG AAAAGCCATT TTACATCGTA
    ATCAATCCGA CAAATGATGA GGTTCCGGAA TTTTTAGCCA GAAACATAAC AGTTGTTGAA
    GGACAAATGA AGAAATTGGA TCCCACTGTA ATAAATGTGT CAGACATGGA TGTGCCACAG
    AACCACTTAT ACTTTACTGT TATAGAGCAG CCCCGACATG GACTGATTAT TGAGGAATCG
    TATATCAGTG GGCACATGAA TAGAAGCCAA TTAATAAAAA GGCATCAGGC AAACAGCCTG
    CCTGTGATAC AAGATTTTTC CATGGACCTG CTAAAAAATG GAATGAAGAT GATATACATG
    CATGATGACT CAGAAAACCT TGCTGACAGC TTCACTATCC AGCTATCTGA TGGAAAGCAT
    GAAGTCAGGA GAACTATTTT TGTGAATGTA ATTGCAGTTA ATGACGAAAA GCCTGTGCTA
    ACCAAGCACT GCGATTTGGT GCTAAGCATG GGGGAACAAA CTCTTATATC AAGTGCCATA
    CTGTCAGCAG AAGATATGGA CACACCCAGG ACAAACATTT CCTATGTTAT TGACAGATGT
    CCAGAGCATG GCCAGCTTCA GATGAAAGTC AATAAAAACT GGGTGACCTT GCATACTGGA
    ATGAAATGCA CTCAGGAGGA CATTGATATG AACCTGGTTA GATATGTGCA TACTGGAACA
    ATGGGCTCAA AAAATGAAGA CACGTTTACT TTCCACCTTT GGGATGGCGG AAACCGATCA
    CCTGAGATAT CTGTTCATAT TTATGTCAAG GATATGGAAA AAGGAGATAT TGCAGTTTTT
    GTGAAAATGC TTAATGTGTC CAAGGGAGAG AGAGTTATCA TAACAACAAA TGTCCTTCTC
    GCCACTGATG GGACCGACAG ACCAGAAGAG CTGCTGTATG TGTTATCCTC GCCACCCCAA
    TATGGGCAGA TTGAATATGT TAAGCATCCT GGAATCCCCA TAACAAGTTT CAGTCAGATG
    GATGTGGCTG CTCACACAAT CTGCTACGTC CACGATTTCA ATTCAGCAGC CACTACTGAT
    ATGTTTAGAT TCCTAGTCAG CAATGGGCTG AAAACGAAAC ATGGGATCAT GAACATCGCA
    CTGGAAACAG TCGATCGAAC TCTTCCAACA CTATCCAGGA ACGATGGTCT AAGACAAGTG
    AGAGGGTCTA TGGAGTGTAT TTCCCCGGCC ACCCTACAGG CACTGGACCC TGACACGTCA
    TCTCAGAACA TTACTTTTCA GTTGGTGGGT CTCCCTCAAT ATGGGCAATT ATACCTCAGA
    GGCTTTCCAC TACAGCAAGA TCACTTCACC CAAAGAGATA TTGACAATCT AGACATTGCC
    TATAAGCATG CTGGACAGGA TGCTCAAATA GACAGGTTTA TGTTTACTGT TACAGACAAA
    GCAAATCAAG GATTTATCAT TAATGGGAAG ATTCAGACGG AACCAGTTCC ATTCACAATA
    CAGGTAGATC CTGTGGACAG CACAGCTCCC AGAATTGTCC ATCTTCAATG CCTTACAGAA
    GTTGAGCTTC TGAAAAATGG CCAGTATGGA ATTTATATTA CTTCAAGACA CTTAAAGGCA
    TCTGATCCAG ACACAGATGA TGAAAGAATT GTCTTTCACA TATTAGGAGG GCCTCACTAT
    GGTTATTTAG AGAATGTTAC AACAGGCAGG TTTATAAGAG AAAGATTTAC ACAAAGGGAT
    TTAAACAGCA AATCAGTGCT CTACGTAATA AATCCATCAG TGGATGTTTT TCATGACAAC
    ATAGAGTTTG AAGTTTCAGA CCCCGTTGGA AACAGAGCTT TACCACAAAT TTTAGAGCTG
    AAATGGAGTC AAATTGAATT GCTCCAAGGC ATGTATGAGG TGTGTGAAAC CGAGGCAGTT
    GTGTCTTTGA AGATGACAAG AAAAGGTTAT CTCCTGGAGT CTGCTTTTGT TTCGCTCAAG
    GTGAATGAAA TAAGTGCTGT TCTTGGCAAA GACATTTCCA TGAATCCTTC CAGACTTATA
    CAGTTTGATC CAGGAGTGTC AACCAAGACT TGGAATATAG TAATTATCCA GGATGGATTA
    GAAGAAGATG ACGAGGTCTT TGAAGTAGTT CTGAGTTCTC CTGTGAATGC TGTTCTTGGC
    TCTAAGACAA GAGCTGTAGT GAAAATTGTG GACCCCAGAG GAGGTGGCTG CAGTTCCTCC
    AAAGTCAGCA TGATCAAAAC AACTGGAATG GGCTTACTAA ACTGTACCGT CAAACCCTCA
    CGCAAACCTT GTGCTGTTTT TAGTTTTAAA GAGACTGCAG ATAAGGTCAG GGAAGATGTC
    CAAGAAGATG TCCATACCTT ACAACTTCCG AGGAAGAGAC TAAGACTCAC AGGGAACAGA
    AAAACAGTTA AACCATCATC AGTATTTAGA AACAAAACAG ACATTATTTA TCAATATCAT
    GGGATTGTGT CTCTCCGAGT GGAAGAAGAT AAGCCAGTAT TAAATGAAAC AAAGAAAGCA
    AAAGTACTAG TGAAGAACCG TGGTCAGAGG AAGATAAATG CCCCCTCCCA AAATCCTGAC
    ATTCTTCAAT CAGGCAGAAC CATGTTGGAT TCTCATGTTA CTACTGGTGG TCAGGATAAC
    GCAATATCGT TTCCTAAAAG ATGCACACCA AGTTTAAAAG GACTCCTGCA TTTTGAGGAC
    ACAGTACAGA AACTGTTCCA GTGTGACGGC ATATCGTGGA ACCCCTGGAT CACTACTAAG
    GATTCTAAAG CAGGAAAATG CCCATTTGGC TGGACTTACC ATGATGGCTA CTGCTATATA
    CTGATCAATG ATCAAAAGGC CACCTGGATA ACAGCAGCCA GAGCATGTAG AGAACAGCAT
    TTTGCTACTC TGGTGGATGT ATTCAGCAAG GAACAAATGG AATGGCTTTG GAATTTCAGT
    GGCAGAAAAT CCTTTTGGAT AGGCCTCAAT GATAGAGTAC GTAATGGAAA CTGGGAGTGG
    AGCAAAGGGG AAATGGTCAC CTTCACCAAT TGGAAAAGAG GCTCTCCTCG ATCTTCACAC
    AAAGGGAAGA ATTGCGTTTT GGCACAAAAG AGAGGCAAAT GGCAGGCTAA AGACTGCAAA
    AAAGGCAAAG GCCATTACTA TATATGTTCA AAAAAGTTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002939136.2
    CDS608..7129
    Translation

    Target ORF information:

    RefSeq Version XM_002939090.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis FRAS1 related extracellular matrix 1 (frem1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002939090.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGAAGCCTT CTATTGTATA CCCAGTTTTG CTGTCAGTGT TGCTCACAAG GGGCCTCTGC 
    GCCTCCTTTA TCAAAGTCAA TCACGGAATG AAAGTTATGA AAGGACAGAG TGCATTCCTC
    TCAGAAAGTG ACCTGCAGTT TTCCATCCCA AGGGAAAAGG ATGCCTGCAA AGTGGAAGTA
    GTTGTTAATG AACCCATTAC ACAGAGAGTT GGAAGGTTAA CACCACAGGT GTTTGACTGC
    CATTTCCTAG CCAATGAAGT TCAGTACACA CACAATGGCT GCCCTATCTT GGATGAAGAC
    GAGGTCATGC TCAGACTTTA CAGATTTACA GAAACTGAGA CTTTTACTGA AACCTTCCTG
    CTGAGAGTGA AGTTAATAGA GCCTGACTGT AATATCATAC AGCTGGGGCC TAAAAAGCTT
    GAAGTGCCAG AGTTTTATGG TCTGTCTGAC GTTATTGATA AGAACATACT GAGCTTTGAC
    TATGACAAAA GAATAAACAT TGAATGTACC ATCAGAATAT CGTCTTCTGA ATCTTTCCTT
    CCTGCACACG GTCAGCTTGT GACGGGGGAT CCTGTGAAAC AAGAACCACG AGGAGATCAG
    CCACACAGCT TCTTCCATGG ATCAAATCAA AAGCCAGGAC CTTTGTGCAA GAATGGAGGC
    TGCATACAAG GTTTAAAGAG GATCCAGAGC GTTATAAAAA TGAGTTGTGA AGATTTCCTT
    GTAAAGGGCA TCCGATACCA GCACTTGGAT CCTCCATCTC CAAATGTTGA TTATATCAGT
    ATCAGGTTGG ATCTTACAGA CTCAAGGAGC AAATCACTTT ACAAGTCAGA ACATGCTTGG
    ATTCCTGTTC TCATTCGGGA TGCTGTTCCA AATCAAATCC CCAAAGCCAA CTTCATGTCT
    ATGTTTATCT TAGAAGTAGA TCAGTTTGTT CTAACACCCA TCTCAACTGC AACACTGGAT
    GCCGAGGACA GCGAAACAGC CAAACATCTT CTGGTCTTCA ATATTACTTT ACCTCCAAGC
    AAAGGGTTCA TCACACACTT GTCTGACCAT ACCAAGCCCA TTACATCCTT TACATGGAAA
    GATCTCAATG AAATGCTCAT AGCATATCAG CCTCCGAACA GCAGCCACAC AGAGAGAAGA
    AATTTGGAGG TGGAGTTTGA GGTGCATGAT TTCTTTTTTG AAAAGAGTCC TCCAATTACA
    GTGCATATCT CTATACGTAC GGCTGACACC AATGCTCCTC GCGTTTCATG GAACATGGGT
    CTGGATTTAC TGGAGGGCCA GTCTCGACCA ATTACATGGA AACAGTTCCA AATTGTGGAT
    AATGACAACA TCAATGCAGT TCGTTTGGTC ATTGTAGATG GATTGCAGCA TGGACGTATT
    ACTCTGAGAG GTGGGAAAGG ATTTATATTC ACAGTTAAGG ACATCCAGGC TGGAGATGTT
    TGCTACCATC ATGATGACAG TGACACTACT AAGGATTTTA TAGTCTTCAG AATATTTGAT
    GGACGTCACA GCATACGACA CAAGTTTCCT ATTAACATCC TACCAAAAGA TGATAGCCCT
    CCCTTCCTCA TCACAAACAT TGTTATTGAG CTTAGTGAAG GTCAAGCTGT CCTTATCGAG
    GGATCTATGC TTTGTGCTTC TGATATGGAC TCTAGTGATG CATACATACG TTTTAATATC
    ACTAAGCCTT CTGTAGCTGG AGAGATTATA AAGAAGCCAG GACAAAATCT TATTGCTTAT
    CCTGTCACAA GCTTCCTGCA ACGAGACTTA TATAACGGTT TCATTTATTA TCGCCATCTT
    GGAGGAGAAG TATTTGAAGA TTCATTTGAT GTTGTTCTGT CTGATAGCCA TGATCCACCA
    AACTTTTCTG GTCCTCAGGC AGTAATCATT CATATTAGCC CCGTGGATGA CCAGCTGCCC
    ATAGAAGCAC CTGGAACAGT ACGACATCTG ATGGTAAAGG AGAATGAGGT TGTTTATATA
    ACAAAGAAAC AACTGCATTT CATAGACCCA GAGTCACCTG AAAGAGAACT TATTTACACA
    GTTGCTACTC CTCCTTGTAT GGTGCCAACC TACAGCAGCT TAGATGCGGG GAAGATAGTT
    CTTGTAGACA CTATACTTAA ACTTGGAAAG GATTATGCAG GAGACACAGC AAGAACATTT
    TCACAGCATG CTGTGAATCA CCTCAAAGTT GCTTACATAC CACCAGCTAA AGACATTGGG
    CCAACTCCTC TACATGTCCA GTTTATATTA TCAGTGAGTA ACCAACATGG AGGAACAGTT
    CAAGGCATCT GTTTCAATAT CACTATCCTT CCAATAGACA ATCAGCCTCC TGAGGTGTTT
    TCATATGATT TGAAAGTTGA AGAAGGTGGT TTCAGCCAAA TTACTGCAAG TAACATTCAC
    ATCACAGATA AGGACACCAG ACAAGAACAA ATTAAAGTTA TTTTACACAA AGCTCCACTG
    CATGGTATCG TTCAACTTGA TGGATCTGCA TTGCAGGAAG GTGACCCATT CACCTGCTTG
    GACCTCATCA CATTAAAAGT TAGGTACCTT CATGATGGTT CAGAGACCCT ACAAGATGAA
    ATCTCATTTA TTGCAACAGA TGGAATCAAT TCATCTGAGT TTATTTTAAA AATACAGGTT
    TCTCCTATTA ATGATGAACC TCCATTTATT AAAGAGAACC TAATTTCCAC CATTATTTGC
    AAAGAAGGGG CAGAGGTAGT CATATCAGCA GACTATATCT CAGCTACTGA TGCAGACAGT
    GATGACATGA AGCTGATATT TATGATCGCT CGCCAACCAC GTTATGGCAT TTTGCTAAAG
    GCTGGATCTA TCATTGATCA ATTCACCCAG AGAGATGTTG TTGAAGGACT GGTACATTAT
    AGACATACAA GTGGCGAAAT TGGCTTGAAG GTCTATGTTG ATATTATAAC ACTGGTGGTT
    TCTGATGCTG AAGCAGATAA ACCGCAGAAA AAGTGCTGCA ACAATGGTCC TCGTCTGCCG
    ATTTTGCCAA ACCATGAGTC TTTTCCTGTA TATGATCTCA ATGTTACAAT TCTGCCAGTG
    GACAACCAAC CCCCATCGGT TATAATAGGT GAAATATTTG TAGTCGATGA GGGCAAATCA
    TCTGCCCTCA GTGTAGATCA TTTAAGTGCC ATGGACCCTG ACACAGAAAG TAACAATCTG
    GAATTCATAC TGGTTTCTCC TCCTCGATAT GGTTACATTG AAAATGTCCT CCCATCTCCT
    GGATTTGAGA AGAGTAATAT GGGTATAAGT ATTGCTGCAT TTCTACTCAA GGACATTAAA
    TCTCTGAACA TCAATTATGT CCAGTCCCGC CATCAAAGAA TTGAACCAAC CACTGATCAG
    TTTACACTGT ATGTCACTGA TGGCAAACTG CGTTCTGTAG AAAAGCCATT TTACATCGTA
    ATCAATCCGA CAAATGATGA GGTTCCGGAA TTTTTAGCCA GAAACATAAC AGTTGTTGAA
    GGACAAATGA AGAAATTGGA TCCCACTGTA ATAAATGTGT CAGACATGGA TGTGCCACAG
    AACCACTTAT ACTTTACTGT TATAGAGCAG CCCCGACATG GACTGATTAT TGAGGAATCG
    TATATCAGTG GGCACATGAA TAGAAGCCAA TTAATAAAAA GGCATCAGGC AAACAGCCTG
    CCTGTGATAC AAGATTTTTC CATGGACCTG CTAAAAAATG GAATGAAGAT GATATACATG
    CATGATGACT CAGAAAACCT TGCTGACAGC TTCACTATCC AGCTATCTGA TGGAAAGCAT
    GAAGTCAGGA GAACTATTTT TGTGAATGTA ATTGCAGTTA ATGACGAAAA GCCTGTGCTA
    ACCAAGCACT GCGATTTGGT GCTAAGCATG GGGGAACAAA CTCTTATATC AAGTGCCATA
    CTGTCAGCAG AAGATATGGA CACACCCAGG ACAAACATTT CCTATGTTAT TGACAGATGT
    CCAGAGCATG GCCAGCTTCA GATGAAAGTC AATAAAAACT GGGTGACCTT GCATACTGGA
    ATGAAATGCA CTCAGGAGGA CATTGATATG AACCTGGTTA GATATGTGCA TACTGGAACA
    ATGGGCTCAA AAAATGAAGA CACGTTTACT TTCCACCTTT GGGATGGCGG AAACCGATCA
    CCTGAGATAT CTGTTCATAT TTATGTCAAG GATATGGAAA AAGGAGATAT TGCAGTTTTT
    GTGAAAATGC TTAATGTGTC CAAGGGAGAG AGAGTTATCA TAACAACAAA TGTCCTTCTC
    GCCACTGATG GGACCGACAG ACCAGAAGAG CTGCTGTATG TGTTATCCTC GCCACCCCAA
    TATGGGCAGA TTGAATATGT TAAGCATCCT GGAATCCCCA TAACAAGTTT CAGTCAGATG
    GATGTGGCTG CTCACACAAT CTGCTACGTC CACGATTTCA ATTCAGCAGC CACTACTGAT
    ATGTTTAGAT TCCTAGTCAG CAATGGGCTG AAAACGAAAC ATGGGATCAT GAACATCGCA
    CTGGAAACAG TCGATCGAAC TCTTCCAACA CTATCCAGGA ACGATGGTCT AAGACAAGTG
    AGAGGGTCTA TGGAGTGTAT TTCCCCGGCC ACCCTACAGG CACTGGACCC TGACACGTCA
    TCTCAGAACA TTACTTTTCA GTTGGTGGGT CTCCCTCAAT ATGGGCAATT ATACCTCAGA
    GGCTTTCCAC TACAGCAAGA TCACTTCACC CAAAGAGATA TTGACAATCT AGACATTGCC
    TATAAGCATG CTGGACAGGA TGCTCAAATA GACAGGTTTA TGTTTACTGT TACAGACAAA
    GCAAATCAAG GATTTATCAT TAATGGGAAG ATTCAGACGG AACCAGTTCC ATTCACAATA
    CAGGTAGATC CTGTGGACAG CACAGCTCCC AGAATTGTCC ATCTTCAATG CCTTACAGAA
    GTTGAGCTTC TGAAAAATGG CCAGTATGGA ATTTATATTA CTTCAAGACA CTTAAAGGCA
    TCTGATCCAG ACACAGATGA TGAAAGAATT GTCTTTCACA TATTAGGAGG GCCTCACTAT
    GGTTATTTAG AGAATGTTAC AACAGGCAGG TTTATAAGAG AAAGATTTAC ACAAAGGGAT
    TTAAACAGCA AATCAGTGCT CTACGTAATA AATCCATCAG TGGATGTTTT TCATGACAAC
    ATAGAGTTTG AAGTTTCAGA CCCCGTTGGA AACAGAGCTT TACCACAAAT TTTAGAGCTG
    AAATGGAGTC AAATTGAATT GCTCCAAGGC ATGTATGAGG TGTGTGAAAC CGAGGCAGTT
    GTGTCTTTGA AGATGACAAG AAAAGGTTAT CTCCTGGAGT CTGCTTTTGT TTCGCTCAAG
    GTGAATGAAA TAAGTGCTGT TCTTGGCAAA GACATTTCCA TGAATCCTTC CAGACTTATA
    CAGTTTGATC CAGGAGTGTC AACCAAGACT TGGAATATAG TAATTATCCA GGATGGATTA
    GAAGAAGATG ACGAGGTCTT TGAAGTAGTT CTGAGTTCTC CTGTGAATGC TGTTCTTGGC
    TCTAAGACAA GAGCTGTAGT GAAAATTGTG GACCCCAGAG GAGGTGGCTG CAGTTCCTCC
    AAAGTCAGCA TGATCAAAAC AACTGGAATG GGCTTACTAA ACTGTACCGT CAAACCCTCA
    CGCAAACCTT GTGCTGTTTT TAGTTTTAAA GAGACTGCAG ATAAGGTCAG GGAAGATGTC
    CAAGAAGATG TCCATACCTT ACAACTTCCG AGGAAGAGAC TAAGACTCAC AGGGAACAGA
    AAAACAGTTA AACCATCATC AGTATTTAGA AACAAAACAG ACATTATTTA TCAATATCAT
    GGGATTGTGT CTCTCCGAGT GGAAGAAGAT AAGCCAGTAT TAAATGAAAC AAAGAAAGCA
    AAAGTACTAG TGAAGAACCG TGGTCAGAGG AAGATAAATG CCCCCTCCCA AAATCCTGAC
    ATTCTTCAAT CAGGCAGAAC CATGTTGGAT TCTCATGTTA CTACTGGTGG TCAGGATAAC
    GCAATATCGT TTCCTAAAAG ATGCACACCA AGTTTAAAAG GACTCCTGCA TTTTGAGGAC
    ACAGTACAGA AACTGTTCCA GTGTGACGGC ATATCGTGGA ACCCCTGGAT CACTACTAAG
    GATTCTAAAG CAGGAAAATG CCCATTTGGC TGGACTTACC ATGATGGCTA CTGCTATATA
    CTGATCAATG ATCAAAAGGC CACCTGGATA ACAGCAGCCA GAGCATGTAG AGAACAGCAT
    TTTGCTACTC TGGTGGATGT ATTCAGCAAG GAACAAATGG AATGGCTTTG GAATTTCAGT
    GGCAGAAAAT CCTTTTGGAT AGGCCTCAAT GATAGAGTAC GTAATGGAAA CTGGGAGTGG
    AGCAAAGGGG AAATGGTCAC CTTCACCAAT TGGAAAAGAG GCTCTCCTCG ATCTTCACAC
    AAAGGGAAGA ATTGCGTTTT GGCACAAAAG AGAGGCAAAT GGCAGGCTAA AGACTGCAAA
    AAAGGCAAAG GCCATTACTA TATATGTTCA AAAAAGTTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.