frem1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	frem1
Entrez Gene ID	100488523
Full Name	FRAS1 related extracellular matrix 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031890521.1	XP_031746381.1	FRAS1-related extracellular matrix protein 1 isoform X2
XM_002939090.4	XP_002939136.2	FRAS1-related extracellular matrix protein 1 isoform X1
XM_002939090.5	XP_002939136.2	FRAS1-related extracellular matrix protein 1 isoform X1
XM_031890523.1	XP_031746383.1	FRAS1-related extracellular matrix protein 1 isoform X3
XM_031890525.1	XP_031746385.1	FRAS1-related extracellular matrix protein 1 isoform X4
XM_018097904.1	XP_017953393.1	FRAS1-related extracellular matrix protein 1 isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	FREM1	XP_520492.3
Canis lupus familiaris (dog)	FREM1	XP_005626693.1
Homo sapiens (human)	FREM1	NP_659403.4
	FREM1	XP_001110764.1
Rattus norvegicus (Norway rat)	Frem1	NP_001178686.1
Bos taurus (cattle)	FREM1	NP_001179924.1
Danio rerio (zebrafish)	frem1a	NP_001177237.1
Mus musculus (house mouse)	Frem1	NP_001185740.1
Gallus gallus (chicken)	FREM1	XP_424932.2
Xenopus tropicalis (tropical clawed frog)	LOC100488523	XP_002939136.2

Gene Ontology

The following frem1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the frem1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa48725	XM_031890525.1 Latest version!	Xenopus tropicalis FRAS1 related extracellular matrix 1 (frem1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa48724	XM_031890523.1 Latest version!	Xenopus tropicalis FRAS1 related extracellular matrix 1 (frem1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa04017	XM_002939090.5 Latest version!	Xenopus tropicalis FRAS1 related extracellular matrix 1 (frem1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa42096	XM_018097904.1 Latest version!	Xenopus tropicalis FRAS1 related extracellular matrix 1 (frem1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OXa48723	XM_031890521.1 Latest version!	Xenopus tropicalis FRAS1 related extracellular matrix 1 (frem1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OXa04017	XM_002939090.4	Xenopus tropicalis FRAS1 related extracellular matrix 1 (frem1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

frem1 ( XM_031890525.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746385.1 Xenopus tropicalis FRAS1 related extracellular matrix 1 (frem1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa48725
Clone ID Related Accession (Same CDS sequence)	XM_031890525.1
Accession Version	XM_031890525.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	FRAS1-related extracellular matrix protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCCTT CTATTGTATA CCCAGTTTTG CTGTCAGTGT TGCTCACAAG GGGCCTCTGC 
GCCTCCTTTA TCAAAGTCAA TCACGGAATG AAAGTTATGA AAGGACAGAG TGCATTCCTC 
TCAGAAAGTG ACCTGCAGTT TTCCATCCCA AGGGAAAAGG ATGCCTGCAA AGTGGAAGTA 
GTTGTTAATG AACCCATTAC ACAGAGAGTT GGAAGGTTAA CACCACAGGT GTTTGACTGC 
CATTTCCTAG CCAATGAAGT TCAGTACACA CACAATGGCT GCCCTATCTT GGATGAAGAC 
GAGGTCATGC TCAGACTTTA CAGATTTACA GAAACTGAGA CTTTTACTGA AACCTTCCTG 
CTGAGAGTGA AGTTAATAGA GCCTGACTGT AATATCATAC AGCTGGGGCC TAAAAAGCTT 
GAAGTGCCAG AGTTTTATGG TCTGTCTGAC GTTATTGATA AGAACATACT GAGCTTTGAC 
TATGACAAAA GAATAAACAT TGAATGTACC ATCAGAATAT CGTCTTCTGA ATCTTTCCTT 
CCTGCACACG GTCAGCTTGT GACGGGGGAT CCTGTGAAAC AAGAACCACG AGGAGATCAG 
CCACACAGCT TCTTCCATGG ATCAAATCAA AAGCCAGGAC CTTTGTGCAA GAATGGAGGC 
TGCATACAAG GTTTAAAGAG GATCCAGAGC GTTATAAAAA TGAGTTGTGA AGATTTCCTT 
GTAAAGGGCA TCCGATACCA GCACTTGGAT CCTCCATCTC CAAATGTTGA TTATATCAGT 
ATCAGGTTGG ATCTTACAGA CTCAAGGAGC AAATCACTTT ACAAGTCAGA ACATGCTTGG 
ATTCCTGTTC TCATTCGGGA TGCTGTTCCA AATCAAATCC CCAAAGCCAA CTTCATGTCT 
ATGTTTATCT TAGAAGTAGA TCAGTTTGTT CTAACACCCA TCTCAACTGC AACACTGGAT 
GCCGAGGACA GCGAAACAGC CAAACATCTT CTGGTCTTCA ATATTACTTT ACCTCCAAGC 
AAAGGGTTCA TCACACACTT GTCTGACCAT ACCAAGCCCA TTACATCCTT TACATGGAAA 
GATCTCAATG AAATGCTCAT AGCATATCAG CCTCCGAACA GCAGCCACAC AGAGAGAAGA 
AATTTGGAGG TGGAGTTTGA GGTGCATGAT TTCTTTTTTG AAAAGAGTCC TCCAATTACA 
GTGCATATCT CTATACGTAC GGCTGACACC AATGCTCCTC GCGTTTCATG GAACATGGGT 
CTGGATTTAC TGGAGGGCCA GTCTCGACCA ATTACATGGA AACAGTTCCA AATTGTGGAT 
AATGACAACA TCAATGCAGT TCGTTTGGTC ATTGTAGATG GATTGCAGCA TGGACGTATT 
ACTCTGAGAG GTGGGAAAGG ATTTATATTC ACAGTTAAGG ACATCCAGGC TGGAGATGTT 
TGCTACCATC ATGATGACAG TGACACTACT AAGGATTTTA TAGTCTTCAG AATATTTGAT 
GGACGTCACA GCATACGACA CAAGTTTCCT ATTAACATCC TACCAAAAGA TGATAGCCCT 
CCCTTCCTCA TCACAAACAT TGTTATTGAG CTTAGTGAAG GTCAAGCTGT CCTTATCGAG 
GGATCTATGC TTTGTGCTTC TGATATGGAC TCTAGTGATG CATACATACG TTTTAATATC 
ACTAAGCCTT CTGTAGCTGG AGAGATTATA AAGAAGCCAG GACAAAATCT TATTGCTTAT 
CCTGTCACAA GCTTCCTGCA ACGAGACTTA TATAACGGTT TCATTTATTA TCGCCATCTT 
GGAGGAGAAG TATTTGAAGA TTCATTTGAT GTTGTTCTGT CTGATAGCCA TGATCCACCA 
AACTTTTCTG GTCCTCAGGC AGTAATCATT CATATTAGCC CCGTGGATGA CCAGCTGCCC 
ATAGAAGCAC CTGGAACAGT ACGACATCTG ATGGTAAAGG AGAATGAGGT TGTTTATATA 
ACAAAGAAAC AACTGCATTT CATAGACCCA GAGTCACCTG AAAGAGAACT TATTTACACA 
GTTGCTACTC CTCCTTGTAT GGTGCCAACC TACAGCAGCT TAGATGCGGG GAAGATAGTT 
CTTGTAGACA CTATACTTAA ACTTGGAAAG GATTATGCAG GAGACACAGC AAGAACATTT 
TCACAGCATG CTGTGAATCA CCTCAAAGTT GCTTACATAC CACCAGCTAA AGACATTGGG 
CCAACTCCTC TACATGTCCA GTTTATATTA TCAGTGAGTA ACCAACATGG AGGAACAGTT 
CAAGGCATCT GTTTCAATAT CACTATCCTT CCAATAGACA ATCAGCCTCC TGAGGTGTTT 
TCATATGATT TGAAAGTTGA AGAAGGTGGT TTCAGCCAAA TTACTGCAAG TAACATTCAC 
ATCACAGATA AGGACACCAG ACAAGAACAA ATTAAAGTTA TTTTACACAA AGCTCCACTG 
CATGGTATCG TTCAACTTGA TGGATCTGCA TTGCAGGAAG GTGACCCATT CACCTGCTTG 
GACCTCATCA CATTAAAAGT ACCTTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746385.1
CDS	620..3169
Translation	MKPSIVYPVLLSVLLTRGLCASFIKVNHGMKVMKGQSAFLSESDLQFSIPREKDACKVEVVVNEPITQRVGRLTPQVFDCHFLANEVQYTHNGCPILDEDEVMLRLYRFTETETFTETFLLRVKLIEPDCNIIQLGPKKLEVPEFYGLSDVIDKNILSFDYDKRINIECTIRISSSESFLPAHGQLVTGDPVKQEPRGDQPHSFFHGSNQKPGPLCKNGGCIQGLKRIQSVIKMSCEDFLVKGIRYQHLDPPSPNVDYISIRLDLTDSRSKSLYKSEHAWIPVLIRDAVPNQIPKANFMSMFILEVDQFVLTPISTATLDAEDSETAKHLLVFNITLPPSKGFITHLSDHTKPITSFTWKDLNEMLIAYQPPNSSHTERRNLEVEFEVHDFFFEKSPPITVHISIRTADTNAPRVSWNMGLDLLEGQSRPITWKQFQIVDNDNINAVRLVIVDGLQHGRITLRGGKGFIFTVKDIQAGDVCYHHDDSDTTKDFIVFRIFDGRHSIRHKFPINILPKDDSPPFLITNIVIELSEGQAVLIEGSMLCASDMDSSDAYIRFNITKPSVAGEIIKKPGQNLIAYPVTSFLQRDLYNGFIYYRHLGGEVFEDSFDVVLSDSHDPPNFSGPQAVIIHISPVDDQLPIEAPGTVRHLMVKENEVVYITKKQLHFIDPESPERELIYTVATPPCMVPTYSSLDAGKIVLVDTILKLGKDYAGDTARTFSQHAVNHLKVAYIPPAKDIGPTPLHVQFILSVSNQHGGTVQGICFNITILPIDNQPPEVFSYDLKVEEGGFSQITASNIHITDKDTRQEQIKVILHKAPLHGIVQLDGSALQEGDPFTCLDLITLKVPS

Target ORF information:

RefSeq Version	XM_031890525.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis FRAS1 related extracellular matrix 1 (frem1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031890525.1

ORF Insert Sequence:

ATGAAGCCTT CTATTGTATA CCCAGTTTTG CTGTCAGTGT TGCTCACAAG GGGCCTCTGC 
GCCTCCTTTA TCAAAGTCAA TCACGGAATG AAAGTTATGA AAGGACAGAG TGCATTCCTC 
TCAGAAAGTG ACCTGCAGTT TTCCATCCCA AGGGAAAAGG ATGCCTGCAA AGTGGAAGTA 
GTTGTTAATG AACCCATTAC ACAGAGAGTT GGAAGGTTAA CACCACAGGT GTTTGACTGC 
CATTTCCTAG CCAATGAAGT TCAGTACACA CACAATGGCT GCCCTATCTT GGATGAAGAC 
GAGGTCATGC TCAGACTTTA CAGATTTACA GAAACTGAGA CTTTTACTGA AACCTTCCTG 
CTGAGAGTGA AGTTAATAGA GCCTGACTGT AATATCATAC AGCTGGGGCC TAAAAAGCTT 
GAAGTGCCAG AGTTTTATGG TCTGTCTGAC GTTATTGATA AGAACATACT GAGCTTTGAC 
TATGACAAAA GAATAAACAT TGAATGTACC ATCAGAATAT CGTCTTCTGA ATCTTTCCTT 
CCTGCACACG GTCAGCTTGT GACGGGGGAT CCTGTGAAAC AAGAACCACG AGGAGATCAG 
CCACACAGCT TCTTCCATGG ATCAAATCAA AAGCCAGGAC CTTTGTGCAA GAATGGAGGC 
TGCATACAAG GTTTAAAGAG GATCCAGAGC GTTATAAAAA TGAGTTGTGA AGATTTCCTT 
GTAAAGGGCA TCCGATACCA GCACTTGGAT CCTCCATCTC CAAATGTTGA TTATATCAGT 
ATCAGGTTGG ATCTTACAGA CTCAAGGAGC AAATCACTTT ACAAGTCAGA ACATGCTTGG 
ATTCCTGTTC TCATTCGGGA TGCTGTTCCA AATCAAATCC CCAAAGCCAA CTTCATGTCT 
ATGTTTATCT TAGAAGTAGA TCAGTTTGTT CTAACACCCA TCTCAACTGC AACACTGGAT 
GCCGAGGACA GCGAAACAGC CAAACATCTT CTGGTCTTCA ATATTACTTT ACCTCCAAGC 
AAAGGGTTCA TCACACACTT GTCTGACCAT ACCAAGCCCA TTACATCCTT TACATGGAAA 
GATCTCAATG AAATGCTCAT AGCATATCAG CCTCCGAACA GCAGCCACAC AGAGAGAAGA 
AATTTGGAGG TGGAGTTTGA GGTGCATGAT TTCTTTTTTG AAAAGAGTCC TCCAATTACA 
GTGCATATCT CTATACGTAC GGCTGACACC AATGCTCCTC GCGTTTCATG GAACATGGGT 
CTGGATTTAC TGGAGGGCCA GTCTCGACCA ATTACATGGA AACAGTTCCA AATTGTGGAT 
AATGACAACA TCAATGCAGT TCGTTTGGTC ATTGTAGATG GATTGCAGCA TGGACGTATT 
ACTCTGAGAG GTGGGAAAGG ATTTATATTC ACAGTTAAGG ACATCCAGGC TGGAGATGTT 
TGCTACCATC ATGATGACAG TGACACTACT AAGGATTTTA TAGTCTTCAG AATATTTGAT 
GGACGTCACA GCATACGACA CAAGTTTCCT ATTAACATCC TACCAAAAGA TGATAGCCCT 
CCCTTCCTCA TCACAAACAT TGTTATTGAG CTTAGTGAAG GTCAAGCTGT CCTTATCGAG 
GGATCTATGC TTTGTGCTTC TGATATGGAC TCTAGTGATG CATACATACG TTTTAATATC 
ACTAAGCCTT CTGTAGCTGG AGAGATTATA AAGAAGCCAG GACAAAATCT TATTGCTTAT 
CCTGTCACAA GCTTCCTGCA ACGAGACTTA TATAACGGTT TCATTTATTA TCGCCATCTT 
GGAGGAGAAG TATTTGAAGA TTCATTTGAT GTTGTTCTGT CTGATAGCCA TGATCCACCA 
AACTTTTCTG GTCCTCAGGC AGTAATCATT CATATTAGCC CCGTGGATGA CCAGCTGCCC 
ATAGAAGCAC CTGGAACAGT ACGACATCTG ATGGTAAAGG AGAATGAGGT TGTTTATATA 
ACAAAGAAAC AACTGCATTT CATAGACCCA GAGTCACCTG AAAGAGAACT TATTTACACA 
GTTGCTACTC CTCCTTGTAT GGTGCCAACC TACAGCAGCT TAGATGCGGG GAAGATAGTT 
CTTGTAGACA CTATACTTAA ACTTGGAAAG GATTATGCAG GAGACACAGC AAGAACATTT 
TCACAGCATG CTGTGAATCA CCTCAAAGTT GCTTACATAC CACCAGCTAA AGACATTGGG 
CCAACTCCTC TACATGTCCA GTTTATATTA TCAGTGAGTA ACCAACATGG AGGAACAGTT 
CAAGGCATCT GTTTCAATAT CACTATCCTT CCAATAGACA ATCAGCCTCC TGAGGTGTTT 
TCATATGATT TGAAAGTTGA AGAAGGTGGT TTCAGCCAAA TTACTGCAAG TAACATTCAC 
ATCACAGATA AGGACACCAG ACAAGAACAA ATTAAAGTTA TTTTACACAA AGCTCCACTG 
CATGGTATCG TTCAACTTGA TGGATCTGCA TTGCAGGAAG GTGACCCATT CACCTGCTTG 
GACCTCATCA CATTAAAAGT ACCTTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

frem1 ( XM_031890523.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746383.1 Xenopus tropicalis FRAS1 related extracellular matrix 1 (frem1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa48724
Clone ID Related Accession (Same CDS sequence)	XM_031890523.1
Accession Version	XM_031890523.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	FRAS1-related extracellular matrix protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCCTT CTATTGTATA CCCAGTTTTG CTGTCAGTGT TGCTCACAAG GGGCCTCTGC 
GCCTCCTTTA TCAAAGTCAA TCACGGAATG AAAGTTATGA AAGGACAGAG TGCATTCCTC 
TCAGAAAGTG ACCTGCAGTT TTCCATCCCA AGGGAAAAGG ATGCCTGCAA AGTGGAAGTA 
GTTGTTAATG AACCCATTAC ACAGAGAGTT GGAAGGTTAA CACCACAGGT GTTTGACTGC 
CATTTCCTAG CCAATGAAGT TCAGTACACA CACAATGGCT GCCCTATCTT GGATGAAGAC 
GAGGTCATGC TCAGACTTTA CAGATTTACA GAAACTGAGA CTTTTACTGA AACCTTCCTG 
CTGAGAGTGA AGTTAATAGA GCCTGACTGT AATATCATAC AGCTGGGGCC TAAAAAGCTT 
GAAGTGCCAG AGTTTTATGG TCTGTCTGAC GTTATTGATA AGAACATACT GAGCTTTGAC 
TATGACAAAA GAATAAACAT TGAATGTACC ATCAGAATAT CGTCTTCTGA ATCTTTCCTT 
CCTGCACACG GTCAGCTTGT GACGGGGGAT CCTGTGAAAC AAGAACCACG AGGAGATCAG 
CCACACAGCT TCTTCCATGG ATCAAATCAA AAGCCAGGAC CTTTGTGCAA GAATGGAGGC 
TGCATACAAG GTTTAAAGAG GATCCAGAGC GTTATAAAAA TGAGTTGTGA AGATTTCCTT 
GTAAAGGGCA TCCGATACCA GCACTTGGAT CCTCCATCTC CAAATGTTGA TTATATCAGT 
ATCAGGTTGG ATCTTACAGA CTCAAGGAGC AAATCACTTT ACAAGTCAGA ACATGCTTGG 
ATTCCTGTTC TCATTCGGGA TGCTGTTCCA AATCAAATCC CCAAAGCCAA CTTCATGTCT 
ATGTTTATCT TAGAAGTAGA TCAGTTTGTT CTAACACCCA TCTCAACTGC AACACTGGAT 
GCCGAGGACA GCGAAACAGC CAAACATCTT CTGGTCTTCA ATATTACTTT ACCTCCAAGC 
AAAGGGTTCA TCACACACTT GTCTGACCAT ACCAAGCCCA TTACATCCTT TACATGGAAA 
GATCTCAATG AAATGCTCAT AGCATATCAG CCTCCGAACA GCAGCCACAC AGAGAGAAGA 
AATTTGGAGG TGGAGTTTGA GGTGCATGAT TTCTTTTTTG AAAAGAGTCC TCCAATTACA 
GTGCATATCT CTATACGTAC GGCTGACACC AATGCTCCTC GCGTTTCATG GAACATGGGT 
CTGGATTTAC TGGAGGGCCA GTCTCGACCA ATTACATGGA AACAGTTCCA AATTGTGGAT 
AATGACAACA TCAATGCAGT TCGTTTGGTC ATTGTAGATG GATTGCAGCA TGGACGTATT 
ACTCTGAGAG GTGGGAAAGG ATTTATATTC ACAGTTAAGG ACATCCAGGC TGGAGATGTT 
TGCTACCATC ATGATGACAG TGACACTACT AAGGATTTTA TAGTCTTCAG AATATTTGAT 
GGACGTCACA GCATACGACA CAAGTTTCCT ATTAACATCC TACCAAAAGA TGATAGCCCT 
CCCTTCCTCA TCACAAACAT TGTTATTGAG CTTAGTGAAG GTCAAGCTGT CCTTATCGAG 
GGATCTATGC TTTGTGCTTC TGATATGGAC TCTAGTGATG CATACATACG TTTTAATATC 
ACTAAGCCTT CTGTAGCTGG AGAGATTATA AAGAAGCCAG GACAAAATCT TATTGCTTAT 
CCTGTCACAA GCTTCCTGCA ACGAGACTTA TATAACGGTT TCATTTATTA TCGCCATCTT 
GGAGGAGAAG TATTTGAAGA TTCATTTGAT GTTGTTCTGT CTGATAGCCA TGATCCACCA 
AACTTTTCTG GTCCTCAGGC AGTAATCATT CATATTAGCC CCGTGGATGA CCAGCTGCCC 
ATAGAAGCAC CTGGAACAGT ACGACATCTG ATGGTAAAGG AGAATGAGGT TGTTTATATA 
ACAAAGAAAC AACTGCATTT CATAGACCCA GAGTCACCTG AAAGAGAACT TATTTACACA 
GTTGCTACTC CTCCTTGTAT GGTGCCAACC TACAGCAGCT TAGATGCGGG GAAGATAGTT 
CTTGTAGACA CTATACTTAA ACTTGGAAAG GATTATGCAG GAGACACAGC AAGAACATTT 
TCACAGCATG CTGTGAATCA CCTCAAAGTT GCTTACATAC CACCAGCTAA AGACATTGGG 
CCAACTCCTC TACATGTCCA GTTTATATTA TCAGTGAGTA ACCAACATGG AGGAACAGTT 
CAAGGCATCT GTTTCAATAT CACTATCCTT CCAATAGACA ATCAGCCTCC TGAGGTGTTT 
TCATATGATT TGAAAGTTGA AGAAGGTGGT TTCAGCCAAA TTACTGCAAG TAACATTCAC 
ATCACAGATA AGGACACCAG ACAAGAACAA ATTAAAGTTA TTTTACACAA AGCTCCACTG 
CATGGTATCG TTCAACTTGA TGGATCTGCA TTGCAGGAAG GTGACCCATT CACCTGCTTG 
GACCTCATCA CATTAAAAGT TAGGTACCTT CATGATGGTT CAGAGACCCT ACAAGATGAA 
ATCTCATTTA TTGCAACAGA TGGAATCAAT TCATCTGAGT TTATTTTAAA AATACAGGTT 
TCTCCTATTA ATGATGAACC TCCATTTATT AAAGAGAACC TAATTTCCAC CATTATTTGC 
AAAGAAGGGG CAGAGGTAGT CATATCAGCA GACTATATCT CAGCTACTGA TGCAGACAGT 
GATGACATGA AGCTGATATT TATGATCGCT CGCCAACCAC GTTATGGCAT TTTGCTAAAG 
GCTGGATCTA TCATTGATCA ATTCACCCAG AGAGATGTTG TTGAAGGACT GGTACATTAT 
AGACATACAA GTGGCGAAAT TGGCTTGAAG GTCTATGTTG ATATTATAAC ACTGGTGGTT 
TCTGATGCTG AAGCAGATAA ACCGCAGAAA AAGTGCTGCA ACAATGGTCC TCGTCTGCCG 
ATTTTGCCAA ACCATGAGTC TTTTCCTGTA TATGATCTCA ATGTTACAAT TCTGCCAGTG 
GACAACCAAC CCCCATCGGT TATAATAGGT GAAATATTTG TAGTCGATGA GGGCAAATCA 
TCTGCCCTCA GTGTAGATCA TTTAAGTGCC ATGGACCCTG ACACAGAAAG TAACAATCTG 
GAATTCATAC TGGTTTCTCC TCCTCGATAT GGTTACATTG AAAATGTCCT CCCATCTCCT 
GGATTTGAGA AGAGTAATAT GGGTATAAGT ATTGCTGCAT TTCTACTCAA GGACATTAAA 
TCTCTGAACA TCAATTATGT CCAGTCCCGC CATCAAAGAA TTGAACCAAC CACTGATCAG 
TTTACACTGT ATGTCACTGA TGGCAAACTG CGTTCTGTAG AAAAGCCATT TTACATCGTA 
ATCAATCCGA CAAATGATGA GGTTCCGGAA TTTTTAGCCA GAAACATAAC AGTTGTTGAA 
GGACAAATGA AGAAATTGGA TCCCACTGTA ATAAATGTGT CAGACATGGA TGTGCCACAG 
AACCACTTAT ACTTTACTGT TATAGAGCAG CCCCGACATG GACTGATTAT TGAGGAATCG 
TATATCAGTG GGCACATGAA TAGAAGCCAA TTAATAAAAA GGCATCAGGC AAACAGCCTG 
CCTGTGATAC AAGATTTTTC CATGGACCTG CTAAAAAATG GAATGAAGAT GATATACATG 
CATGATGACT CAGAAAACCT TGCTGACAGC TTCACTATCC AGCTATCTGA TGGAAAGCAT 
GAAGTCAGGA GAACTATTTT TGTGAATGTA ATTGCAGTTA ATGACGAAAA GCCTGTGCTA 
ACCAAGCACT GCGATTTGGT GCTAAGCATG GGGGAACAAA CTCTTATATC AAGTGCCATA 
CTGTCAGCAG AAGATATGGA CACACCCAGG ACAAACATTT CCTATGTTAT TGACAGATGT 
CCAGAGCATG GCCAGCTTCA GATGAAAGTC AATAAAAACT GGGTGACCTT GCATACTGGA 
ATGAAATGCA CTCAGGAGGA CATTGATATG AACCTGGTTA GATATGTGCA TACTGGAACA 
ATGGGCTCAA AAAATGAAGA CACGTTTACT TTCCACCTTT GGGATGGCGG AAACCGATCA 
CCTGAGATAT CTGTTCATAT TTATGTCAAG GATATGGAAA AAGGAGATAT TGCAGTTTTT 
GTGAAAATGC TTAATGTGTC CAAGGGAGAG AGAGTTATCA TAACAACAAA TGTCCTTCTC 
GCCACTGATG GGACCGACAG ACCAGAAGAG CTGCTGTATG TGTTATCCTC GCCACCCCAA 
TATGGGCAGA TTGAATATGT TAAGCATCCT GGAATCCCCA TAACAAGTTT CAGTCAGATG 
GATGTGGCTG CTCACACAAT CTGCTACGTC CACGATTTCA ATTCAGCAGC CACTACTGAT 
ATGTTTAGAT GGGGAAACAA GAACCAATGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746383.1
CDS	618..5090
Translation	MKPSIVYPVLLSVLLTRGLCASFIKVNHGMKVMKGQSAFLSESDLQFSIPREKDACKVEVVVNEPITQRVGRLTPQVFDCHFLANEVQYTHNGCPILDEDEVMLRLYRFTETETFTETFLLRVKLIEPDCNIIQLGPKKLEVPEFYGLSDVIDKNILSFDYDKRINIECTIRISSSESFLPAHGQLVTGDPVKQEPRGDQPHSFFHGSNQKPGPLCKNGGCIQGLKRIQSVIKMSCEDFLVKGIRYQHLDPPSPNVDYISIRLDLTDSRSKSLYKSEHAWIPVLIRDAVPNQIPKANFMSMFILEVDQFVLTPISTATLDAEDSETAKHLLVFNITLPPSKGFITHLSDHTKPITSFTWKDLNEMLIAYQPPNSSHTERRNLEVEFEVHDFFFEKSPPITVHISIRTADTNAPRVSWNMGLDLLEGQSRPITWKQFQIVDNDNINAVRLVIVDGLQHGRITLRGGKGFIFTVKDIQAGDVCYHHDDSDTTKDFIVFRIFDGRHSIRHKFPINILPKDDSPPFLITNIVIELSEGQAVLIEGSMLCASDMDSSDAYIRFNITKPSVAGEIIKKPGQNLIAYPVTSFLQRDLYNGFIYYRHLGGEVFEDSFDVVLSDSHDPPNFSGPQAVIIHISPVDDQLPIEAPGTVRHLMVKENEVVYITKKQLHFIDPESPERELIYTVATPPCMVPTYSSLDAGKIVLVDTILKLGKDYAGDTARTFSQHAVNHLKVAYIPPAKDIGPTPLHVQFILSVSNQHGGTVQGICFNITILPIDNQPPEVFSYDLKVEEGGFSQITASNIHITDKDTRQEQIKVILHKAPLHGIVQLDGSALQEGDPFTCLDLITLKVRYLHDGSETLQDEISFIATDGINSSEFILKIQVSPINDEPPFIKENLISTIICKEGAEVVISADYISATDADSDDMKLIFMIARQPRYGILLKAGSIIDQFTQRDVVEGLVHYRHTSGEIGLKVYVDIITLVVSDAEADKPQKKCCNNGPRLPILPNHESFPVYDLNVTILPVDNQPPSVIIGEIFVVDEGKSSALSVDHLSAMDPDTESNNLEFILVSPPRYGYIENVLPSPGFEKSNMGISIAAFLLKDIKSLNINYVQSRHQRIEPTTDQFTLYVTDGKLRSVEKPFYIVINPTNDEVPEFLARNITVVEGQMKKLDPTVINVSDMDVPQNHLYFTVIEQPRHGLIIEESYISGHMNRSQLIKRHQANSLPVIQDFSMDLLKNGMKMIYMHDDSENLADSFTIQLSDGKHEVRRTIFVNVIAVNDEKPVLTKHCDLVLSMGEQTLISSAILSAEDMDTPRTNISYVIDRCPEHGQLQMKVNKNWVTLHTGMKCTQEDIDMNLVRYVHTGTMGSKNEDTFTFHLWDGGNRSPEISVHIYVKDMEKGDIAVFVKMLNVSKGERVIITTNVLLATDGTDRPEELLYVLSSPPQYGQIEYVKHPGIPITSFSQMDVAAHTICYVHDFNSAATTDMFRWGNKNQC

Target ORF information:

RefSeq Version	XM_031890523.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis FRAS1 related extracellular matrix 1 (frem1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031890523.1

ORF Insert Sequence:

ATGAAGCCTT CTATTGTATA CCCAGTTTTG CTGTCAGTGT TGCTCACAAG GGGCCTCTGC 
GCCTCCTTTA TCAAAGTCAA TCACGGAATG AAAGTTATGA AAGGACAGAG TGCATTCCTC 
TCAGAAAGTG ACCTGCAGTT TTCCATCCCA AGGGAAAAGG ATGCCTGCAA AGTGGAAGTA 
GTTGTTAATG AACCCATTAC ACAGAGAGTT GGAAGGTTAA CACCACAGGT GTTTGACTGC 
CATTTCCTAG CCAATGAAGT TCAGTACACA CACAATGGCT GCCCTATCTT GGATGAAGAC 
GAGGTCATGC TCAGACTTTA CAGATTTACA GAAACTGAGA CTTTTACTGA AACCTTCCTG 
CTGAGAGTGA AGTTAATAGA GCCTGACTGT AATATCATAC AGCTGGGGCC TAAAAAGCTT 
GAAGTGCCAG AGTTTTATGG TCTGTCTGAC GTTATTGATA AGAACATACT GAGCTTTGAC 
TATGACAAAA GAATAAACAT TGAATGTACC ATCAGAATAT CGTCTTCTGA ATCTTTCCTT 
CCTGCACACG GTCAGCTTGT GACGGGGGAT CCTGTGAAAC AAGAACCACG AGGAGATCAG 
CCACACAGCT TCTTCCATGG ATCAAATCAA AAGCCAGGAC CTTTGTGCAA GAATGGAGGC 
TGCATACAAG GTTTAAAGAG GATCCAGAGC GTTATAAAAA TGAGTTGTGA AGATTTCCTT 
GTAAAGGGCA TCCGATACCA GCACTTGGAT CCTCCATCTC CAAATGTTGA TTATATCAGT 
ATCAGGTTGG ATCTTACAGA CTCAAGGAGC AAATCACTTT ACAAGTCAGA ACATGCTTGG 
ATTCCTGTTC TCATTCGGGA TGCTGTTCCA AATCAAATCC CCAAAGCCAA CTTCATGTCT 
ATGTTTATCT TAGAAGTAGA TCAGTTTGTT CTAACACCCA TCTCAACTGC AACACTGGAT 
GCCGAGGACA GCGAAACAGC CAAACATCTT CTGGTCTTCA ATATTACTTT ACCTCCAAGC 
AAAGGGTTCA TCACACACTT GTCTGACCAT ACCAAGCCCA TTACATCCTT TACATGGAAA 
GATCTCAATG AAATGCTCAT AGCATATCAG CCTCCGAACA GCAGCCACAC AGAGAGAAGA 
AATTTGGAGG TGGAGTTTGA GGTGCATGAT TTCTTTTTTG AAAAGAGTCC TCCAATTACA 
GTGCATATCT CTATACGTAC GGCTGACACC AATGCTCCTC GCGTTTCATG GAACATGGGT 
CTGGATTTAC TGGAGGGCCA GTCTCGACCA ATTACATGGA AACAGTTCCA AATTGTGGAT 
AATGACAACA TCAATGCAGT TCGTTTGGTC ATTGTAGATG GATTGCAGCA TGGACGTATT 
ACTCTGAGAG GTGGGAAAGG ATTTATATTC ACAGTTAAGG ACATCCAGGC TGGAGATGTT 
TGCTACCATC ATGATGACAG TGACACTACT AAGGATTTTA TAGTCTTCAG AATATTTGAT 
GGACGTCACA GCATACGACA CAAGTTTCCT ATTAACATCC TACCAAAAGA TGATAGCCCT 
CCCTTCCTCA TCACAAACAT TGTTATTGAG CTTAGTGAAG GTCAAGCTGT CCTTATCGAG 
GGATCTATGC TTTGTGCTTC TGATATGGAC TCTAGTGATG CATACATACG TTTTAATATC 
ACTAAGCCTT CTGTAGCTGG AGAGATTATA AAGAAGCCAG GACAAAATCT TATTGCTTAT 
CCTGTCACAA GCTTCCTGCA ACGAGACTTA TATAACGGTT TCATTTATTA TCGCCATCTT 
GGAGGAGAAG TATTTGAAGA TTCATTTGAT GTTGTTCTGT CTGATAGCCA TGATCCACCA 
AACTTTTCTG GTCCTCAGGC AGTAATCATT CATATTAGCC CCGTGGATGA CCAGCTGCCC 
ATAGAAGCAC CTGGAACAGT ACGACATCTG ATGGTAAAGG AGAATGAGGT TGTTTATATA 
ACAAAGAAAC AACTGCATTT CATAGACCCA GAGTCACCTG AAAGAGAACT TATTTACACA 
GTTGCTACTC CTCCTTGTAT GGTGCCAACC TACAGCAGCT TAGATGCGGG GAAGATAGTT 
CTTGTAGACA CTATACTTAA ACTTGGAAAG GATTATGCAG GAGACACAGC AAGAACATTT 
TCACAGCATG CTGTGAATCA CCTCAAAGTT GCTTACATAC CACCAGCTAA AGACATTGGG 
CCAACTCCTC TACATGTCCA GTTTATATTA TCAGTGAGTA ACCAACATGG AGGAACAGTT 
CAAGGCATCT GTTTCAATAT CACTATCCTT CCAATAGACA ATCAGCCTCC TGAGGTGTTT 
TCATATGATT TGAAAGTTGA AGAAGGTGGT TTCAGCCAAA TTACTGCAAG TAACATTCAC 
ATCACAGATA AGGACACCAG ACAAGAACAA ATTAAAGTTA TTTTACACAA AGCTCCACTG 
CATGGTATCG TTCAACTTGA TGGATCTGCA TTGCAGGAAG GTGACCCATT CACCTGCTTG 
GACCTCATCA CATTAAAAGT TAGGTACCTT CATGATGGTT CAGAGACCCT ACAAGATGAA 
ATCTCATTTA TTGCAACAGA TGGAATCAAT TCATCTGAGT TTATTTTAAA AATACAGGTT 
TCTCCTATTA ATGATGAACC TCCATTTATT AAAGAGAACC TAATTTCCAC CATTATTTGC 
AAAGAAGGGG CAGAGGTAGT CATATCAGCA GACTATATCT CAGCTACTGA TGCAGACAGT 
GATGACATGA AGCTGATATT TATGATCGCT CGCCAACCAC GTTATGGCAT TTTGCTAAAG 
GCTGGATCTA TCATTGATCA ATTCACCCAG AGAGATGTTG TTGAAGGACT GGTACATTAT 
AGACATACAA GTGGCGAAAT TGGCTTGAAG GTCTATGTTG ATATTATAAC ACTGGTGGTT 
TCTGATGCTG AAGCAGATAA ACCGCAGAAA AAGTGCTGCA ACAATGGTCC TCGTCTGCCG 
ATTTTGCCAA ACCATGAGTC TTTTCCTGTA TATGATCTCA ATGTTACAAT TCTGCCAGTG 
GACAACCAAC CCCCATCGGT TATAATAGGT GAAATATTTG TAGTCGATGA GGGCAAATCA 
TCTGCCCTCA GTGTAGATCA TTTAAGTGCC ATGGACCCTG ACACAGAAAG TAACAATCTG 
GAATTCATAC TGGTTTCTCC TCCTCGATAT GGTTACATTG AAAATGTCCT CCCATCTCCT 
GGATTTGAGA AGAGTAATAT GGGTATAAGT ATTGCTGCAT TTCTACTCAA GGACATTAAA 
TCTCTGAACA TCAATTATGT CCAGTCCCGC CATCAAAGAA TTGAACCAAC CACTGATCAG 
TTTACACTGT ATGTCACTGA TGGCAAACTG CGTTCTGTAG AAAAGCCATT TTACATCGTA 
ATCAATCCGA CAAATGATGA GGTTCCGGAA TTTTTAGCCA GAAACATAAC AGTTGTTGAA 
GGACAAATGA AGAAATTGGA TCCCACTGTA ATAAATGTGT CAGACATGGA TGTGCCACAG 
AACCACTTAT ACTTTACTGT TATAGAGCAG CCCCGACATG GACTGATTAT TGAGGAATCG 
TATATCAGTG GGCACATGAA TAGAAGCCAA TTAATAAAAA GGCATCAGGC AAACAGCCTG 
CCTGTGATAC AAGATTTTTC CATGGACCTG CTAAAAAATG GAATGAAGAT GATATACATG 
CATGATGACT CAGAAAACCT TGCTGACAGC TTCACTATCC AGCTATCTGA TGGAAAGCAT 
GAAGTCAGGA GAACTATTTT TGTGAATGTA ATTGCAGTTA ATGACGAAAA GCCTGTGCTA 
ACCAAGCACT GCGATTTGGT GCTAAGCATG GGGGAACAAA CTCTTATATC AAGTGCCATA 
CTGTCAGCAG AAGATATGGA CACACCCAGG ACAAACATTT CCTATGTTAT TGACAGATGT 
CCAGAGCATG GCCAGCTTCA GATGAAAGTC AATAAAAACT GGGTGACCTT GCATACTGGA 
ATGAAATGCA CTCAGGAGGA CATTGATATG AACCTGGTTA GATATGTGCA TACTGGAACA 
ATGGGCTCAA AAAATGAAGA CACGTTTACT TTCCACCTTT GGGATGGCGG AAACCGATCA 
CCTGAGATAT CTGTTCATAT TTATGTCAAG GATATGGAAA AAGGAGATAT TGCAGTTTTT 
GTGAAAATGC TTAATGTGTC CAAGGGAGAG AGAGTTATCA TAACAACAAA TGTCCTTCTC 
GCCACTGATG GGACCGACAG ACCAGAAGAG CTGCTGTATG TGTTATCCTC GCCACCCCAA 
TATGGGCAGA TTGAATATGT TAAGCATCCT GGAATCCCCA TAACAAGTTT CAGTCAGATG 
GATGTGGCTG CTCACACAAT CTGCTACGTC CACGATTTCA ATTCAGCAGC CACTACTGAT 
ATGTTTAGAT GGGGAAACAA GAACCAATGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

frem1 ( XM_002939090.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002939136.2 Xenopus tropicalis FRAS1 related extracellular matrix 1 (frem1), transcript variant X1, mRNA.

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CloneID	OXa04017
Clone ID Related Accession (Same CDS sequence)	XM_002939090.4 , XM_002939090.5
Accession Version	XM_002939090.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	FRAS1-related extracellular matrix protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002939090.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCCTT CTATTGTATA CCCAGTTTTG CTGTCAGTGT TGCTCACAAG GGGCCTCTGC 
GCCTCCTTTA TCAAAGTCAA TCACGGAATG AAAGTTATGA AAGGACAGAG TGCATTCCTC 
TCAGAAAGTG ACCTGCAGTT TTCCATCCCA AGGGAAAAGG ATGCCTGCAA AGTGGAAGTA 
GTTGTTAATG AACCCATTAC ACAGAGAGTT GGAAGGTTAA CACCACAGGT GTTTGACTGC 
CATTTCCTAG CCAATGAAGT TCAGTACACA CACAATGGCT GCCCTATCTT GGATGAAGAC 
GAGGTCATGC TCAGACTTTA CAGATTTACA GAAACTGAGA CTTTTACTGA AACCTTCCTG 
CTGAGAGTGA AGTTAATAGA GCCTGACTGT AATATCATAC AGCTGGGGCC TAAAAAGCTT 
GAAGTGCCAG AGTTTTATGG TCTGTCTGAC GTTATTGATA AGAACATACT GAGCTTTGAC 
TATGACAAAA GAATAAACAT TGAATGTACC ATCAGAATAT CGTCTTCTGA ATCTTTCCTT 
CCTGCACACG GTCAGCTTGT GACGGGGGAT CCTGTGAAAC AAGAACCACG AGGAGATCAG 
CCACACAGCT TCTTCCATGG ATCAAATCAA AAGCCAGGAC CTTTGTGCAA GAATGGAGGC 
TGCATACAAG GTTTAAAGAG GATCCAGAGC GTTATAAAAA TGAGTTGTGA AGATTTCCTT 
GTAAAGGGCA TCCGATACCA GCACTTGGAT CCTCCATCTC CAAATGTTGA TTATATCAGT 
ATCAGGTTGG ATCTTACAGA CTCAAGGAGC AAATCACTTT ACAAGTCAGA ACATGCTTGG 
ATTCCTGTTC TCATTCGGGA TGCTGTTCCA AATCAAATCC CCAAAGCCAA CTTCATGTCT 
ATGTTTATCT TAGAAGTAGA TCAGTTTGTT CTAACACCCA TCTCAACTGC AACACTGGAT 
GCCGAGGACA GCGAAACAGC CAAACATCTT CTGGTCTTCA ATATTACTTT ACCTCCAAGC 
AAAGGGTTCA TCACACACTT GTCTGACCAT ACCAAGCCCA TTACATCCTT TACATGGAAA 
GATCTCAATG AAATGCTCAT AGCATATCAG CCTCCGAACA GCAGCCACAC AGAGAGAAGA 
AATTTGGAGG TGGAGTTTGA GGTGCATGAT TTCTTTTTTG AAAAGAGTCC TCCAATTACA 
GTGCATATCT CTATACGTAC GGCTGACACC AATGCTCCTC GCGTTTCATG GAACATGGGT 
CTGGATTTAC TGGAGGGCCA GTCTCGACCA ATTACATGGA AACAGTTCCA AATTGTGGAT 
AATGACAACA TCAATGCAGT TCGTTTGGTC ATTGTAGATG GATTGCAGCA TGGACGTATT 
ACTCTGAGAG GTGGGAAAGG ATTTATATTC ACAGTTAAGG ACATCCAGGC TGGAGATGTT 
TGCTACCATC ATGATGACAG TGACACTACT AAGGATTTTA TAGTCTTCAG AATATTTGAT 
GGACGTCACA GCATACGACA CAAGTTTCCT ATTAACATCC TACCAAAAGA TGATAGCCCT 
CCCTTCCTCA TCACAAACAT TGTTATTGAG CTTAGTGAAG GTCAAGCTGT CCTTATCGAG 
GGATCTATGC TTTGTGCTTC TGATATGGAC TCTAGTGATG CATACATACG TTTTAATATC 
ACTAAGCCTT CTGTAGCTGG AGAGATTATA AAGAAGCCAG GACAAAATCT TATTGCTTAT 
CCTGTCACAA GCTTCCTGCA ACGAGACTTA TATAACGGTT TCATTTATTA TCGCCATCTT 
GGAGGAGAAG TATTTGAAGA TTCATTTGAT GTTGTTCTGT CTGATAGCCA TGATCCACCA 
AACTTTTCTG GTCCTCAGGC AGTAATCATT CATATTAGCC CCGTGGATGA CCAGCTGCCC 
ATAGAAGCAC CTGGAACAGT ACGACATCTG ATGGTAAAGG AGAATGAGGT TGTTTATATA 
ACAAAGAAAC AACTGCATTT CATAGACCCA GAGTCACCTG AAAGAGAACT TATTTACACA 
GTTGCTACTC CTCCTTGTAT GGTGCCAACC TACAGCAGCT TAGATGCGGG GAAGATAGTT 
CTTGTAGACA CTATACTTAA ACTTGGAAAG GATTATGCAG GAGACACAGC AAGAACATTT 
TCACAGCATG CTGTGAATCA CCTCAAAGTT GCTTACATAC CACCAGCTAA AGACATTGGG 
CCAACTCCTC TACATGTCCA GTTTATATTA TCAGTGAGTA ACCAACATGG AGGAACAGTT 
CAAGGCATCT GTTTCAATAT CACTATCCTT CCAATAGACA ATCAGCCTCC TGAGGTGTTT 
TCATATGATT TGAAAGTTGA AGAAGGTGGT TTCAGCCAAA TTACTGCAAG TAACATTCAC 
ATCACAGATA AGGACACCAG ACAAGAACAA ATTAAAGTTA TTTTACACAA AGCTCCACTG 
CATGGTATCG TTCAACTTGA TGGATCTGCA TTGCAGGAAG GTGACCCATT CACCTGCTTG 
GACCTCATCA CATTAAAAGT TAGGTACCTT CATGATGGTT CAGAGACCCT ACAAGATGAA 
ATCTCATTTA TTGCAACAGA TGGAATCAAT TCATCTGAGT TTATTTTAAA AATACAGGTT 
TCTCCTATTA ATGATGAACC TCCATTTATT AAAGAGAACC TAATTTCCAC CATTATTTGC 
AAAGAAGGGG CAGAGGTAGT CATATCAGCA GACTATATCT CAGCTACTGA TGCAGACAGT 
GATGACATGA AGCTGATATT TATGATCGCT CGCCAACCAC GTTATGGCAT TTTGCTAAAG 
GCTGGATCTA TCATTGATCA ATTCACCCAG AGAGATGTTG TTGAAGGACT GGTACATTAT 
AGACATACAA GTGGCGAAAT TGGCTTGAAG GTCTATGTTG ATATTATAAC ACTGGTGGTT 
TCTGATGCTG AAGCAGATAA ACCGCAGAAA AAGTGCTGCA ACAATGGTCC TCGTCTGCCG 
ATTTTGCCAA ACCATGAGTC TTTTCCTGTA TATGATCTCA ATGTTACAAT TCTGCCAGTG 
GACAACCAAC CCCCATCGGT TATAATAGGT GAAATATTTG TAGTCGATGA GGGCAAATCA 
TCTGCCCTCA GTGTAGATCA TTTAAGTGCC ATGGACCCTG ACACAGAAAG TAACAATCTG 
GAATTCATAC TGGTTTCTCC TCCTCGATAT GGTTACATTG AAAATGTCCT CCCATCTCCT 
GGATTTGAGA AGAGTAATAT GGGTATAAGT ATTGCTGCAT TTCTACTCAA GGACATTAAA 
TCTCTGAACA TCAATTATGT CCAGTCCCGC CATCAAAGAA TTGAACCAAC CACTGATCAG 
TTTACACTGT ATGTCACTGA TGGCAAACTG CGTTCTGTAG AAAAGCCATT TTACATCGTA 
ATCAATCCGA CAAATGATGA GGTTCCGGAA TTTTTAGCCA GAAACATAAC AGTTGTTGAA 
GGACAAATGA AGAAATTGGA TCCCACTGTA ATAAATGTGT CAGACATGGA TGTGCCACAG 
AACCACTTAT ACTTTACTGT TATAGAGCAG CCCCGACATG GACTGATTAT TGAGGAATCG 
TATATCAGTG GGCACATGAA TAGAAGCCAA TTAATAAAAA GGCATCAGGC AAACAGCCTG 
CCTGTGATAC AAGATTTTTC CATGGACCTG CTAAAAAATG GAATGAAGAT GATATACATG 
CATGATGACT CAGAAAACCT TGCTGACAGC TTCACTATCC AGCTATCTGA TGGAAAGCAT 
GAAGTCAGGA GAACTATTTT TGTGAATGTA ATTGCAGTTA ATGACGAAAA GCCTGTGCTA 
ACCAAGCACT GCGATTTGGT GCTAAGCATG GGGGAACAAA CTCTTATATC AAGTGCCATA 
CTGTCAGCAG AAGATATGGA CACACCCAGG ACAAACATTT CCTATGTTAT TGACAGATGT 
CCAGAGCATG GCCAGCTTCA GATGAAAGTC AATAAAAACT GGGTGACCTT GCATACTGGA 
ATGAAATGCA CTCAGGAGGA CATTGATATG AACCTGGTTA GATATGTGCA TACTGGAACA 
ATGGGCTCAA AAAATGAAGA CACGTTTACT TTCCACCTTT GGGATGGCGG AAACCGATCA 
CCTGAGATAT CTGTTCATAT TTATGTCAAG GATATGGAAA AAGGAGATAT TGCAGTTTTT 
GTGAAAATGC TTAATGTGTC CAAGGGAGAG AGAGTTATCA TAACAACAAA TGTCCTTCTC 
GCCACTGATG GGACCGACAG ACCAGAAGAG CTGCTGTATG TGTTATCCTC GCCACCCCAA 
TATGGGCAGA TTGAATATGT TAAGCATCCT GGAATCCCCA TAACAAGTTT CAGTCAGATG 
GATGTGGCTG CTCACACAAT CTGCTACGTC CACGATTTCA ATTCAGCAGC CACTACTGAT 
ATGTTTAGAT TCCTAGTCAG CAATGGGCTG AAAACGAAAC ATGGGATCAT GAACATCGCA 
CTGGAAACAG TCGATCGAAC TCTTCCAACA CTATCCAGGA ACGATGGTCT AAGACAAGTG 
AGAGGGTCTA TGGAGTGTAT TTCCCCGGCC ACCCTACAGG CACTGGACCC TGACACGTCA 
TCTCAGAACA TTACTTTTCA GTTGGTGGGT CTCCCTCAAT ATGGGCAATT ATACCTCAGA 
GGCTTTCCAC TACAGCAAGA TCACTTCACC CAAAGAGATA TTGACAATCT AGACATTGCC 
TATAAGCATG CTGGACAGGA TGCTCAAATA GACAGGTTTA TGTTTACTGT TACAGACAAA 
GCAAATCAAG GATTTATCAT TAATGGGAAG ATTCAGACGG AACCAGTTCC ATTCACAATA 
CAGGTAGATC CTGTGGACAG CACAGCTCCC AGAATTGTCC ATCTTCAATG CCTTACAGAA 
GTTGAGCTTC TGAAAAATGG CCAGTATGGA ATTTATATTA CTTCAAGACA CTTAAAGGCA 
TCTGATCCAG ACACAGATGA TGAAAGAATT GTCTTTCACA TATTAGGAGG GCCTCACTAT 
GGTTATTTAG AGAATGTTAC AACAGGCAGG TTTATAAGAG AAAGATTTAC ACAAAGGGAT 
TTAAACAGCA AATCAGTGCT CTACGTAATA AATCCATCAG TGGATGTTTT TCATGACAAC 
ATAGAGTTTG AAGTTTCAGA CCCCGTTGGA AACAGAGCTT TACCACAAAT TTTAGAGCTG 
AAATGGAGTC AAATTGAATT GCTCCAAGGC ATGTATGAGG TGTGTGAAAC CGAGGCAGTT 
GTGTCTTTGA AGATGACAAG AAAAGGTTAT CTCCTGGAGT CTGCTTTTGT TTCGCTCAAG 
GTGAATGAAA TAAGTGCTGT TCTTGGCAAA GACATTTCCA TGAATCCTTC CAGACTTATA 
CAGTTTGATC CAGGAGTGTC AACCAAGACT TGGAATATAG TAATTATCCA GGATGGATTA 
GAAGAAGATG ACGAGGTCTT TGAAGTAGTT CTGAGTTCTC CTGTGAATGC TGTTCTTGGC 
TCTAAGACAA GAGCTGTAGT GAAAATTGTG GACCCCAGAG GAGGTGGCTG CAGTTCCTCC 
AAAGTCAGCA TGATCAAAAC AACTGGAATG GGCTTACTAA ACTGTACCGT CAAACCCTCA 
CGCAAACCTT GTGCTGTTTT TAGTTTTAAA GAGACTGCAG ATAAGGTCAG GGAAGATGTC 
CAAGAAGATG TCCATACCTT ACAACTTCCG AGGAAGAGAC TAAGACTCAC AGGGAACAGA 
AAAACAGTTA AACCATCATC AGTATTTAGA AACAAAACAG ACATTATTTA TCAATATCAT 
GGGATTGTGT CTCTCCGAGT GGAAGAAGAT AAGCCAGTAT TAAATGAAAC AAAGAAAGCA 
AAAGTACTAG TGAAGAACCG TGGTCAGAGG AAGATAAATG CCCCCTCCCA AAATCCTGAC 
ATTCTTCAAT CAGGCAGAAC CATGTTGGAT TCTCATGTTA CTACTGGTGG TCAGGATAAC 
GCAATATCGT TTCCTAAAAG ATGCACACCA AGTTTAAAAG GACTCCTGCA TTTTGAGGAC 
ACAGTACAGA AACTGTTCCA GTGTGACGGC ATATCGTGGA ACCCCTGGAT CACTACTAAG 
GATTCTAAAG CAGGAAAATG CCCATTTGGC TGGACTTACC ATGATGGCTA CTGCTATATA 
CTGATCAATG ATCAAAAGGC CACCTGGATA ACAGCAGCCA GAGCATGTAG AGAACAGCAT 
TTTGCTACTC TGGTGGATGT ATTCAGCAAG GAACAAATGG AATGGCTTTG GAATTTCAGT 
GGCAGAAAAT CCTTTTGGAT AGGCCTCAAT GATAGAGTAC GTAATGGAAA CTGGGAGTGG 
AGCAAAGGGG AAATGGTCAC CTTCACCAAT TGGAAAAGAG GCTCTCCTCG ATCTTCACAC 
AAAGGGAAGA ATTGCGTTTT GGCACAAAAG AGAGGCAAAT GGCAGGCTAA AGACTGCAAA 
AAAGGCAAAG GCCATTACTA TATATGTTCA AAAAAGTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002939136.2
CDS	613..7134
Translation	MKPSIVYPVLLSVLLTRGLCASFIKVNHGMKVMKGQSAFLSESDLQFSIPREKDACKVEVVVNEPITQRVGRLTPQVFDCHFLANEVQYTHNGCPILDEDEVMLRLYRFTETETFTETFLLRVKLIEPDCNIIQLGPKKLEVPEFYGLSDVIDKNILSFDYDKRINIECTIRISSSESFLPAHGQLVTGDPVKQEPRGDQPHSFFHGSNQKPGPLCKNGGCIQGLKRIQSVIKMSCEDFLVKGIRYQHLDPPSPNVDYISIRLDLTDSRSKSLYKSEHAWIPVLIRDAVPNQIPKANFMSMFILEVDQFVLTPISTATLDAEDSETAKHLLVFNITLPPSKGFITHLSDHTKPITSFTWKDLNEMLIAYQPPNSSHTERRNLEVEFEVHDFFFEKSPPITVHISIRTADTNAPRVSWNMGLDLLEGQSRPITWKQFQIVDNDNINAVRLVIVDGLQHGRITLRGGKGFIFTVKDIQAGDVCYHHDDSDTTKDFIVFRIFDGRHSIRHKFPINILPKDDSPPFLITNIVIELSEGQAVLIEGSMLCASDMDSSDAYIRFNITKPSVAGEIIKKPGQNLIAYPVTSFLQRDLYNGFIYYRHLGGEVFEDSFDVVLSDSHDPPNFSGPQAVIIHISPVDDQLPIEAPGTVRHLMVKENEVVYITKKQLHFIDPESPERELIYTVATPPCMVPTYSSLDAGKIVLVDTILKLGKDYAGDTARTFSQHAVNHLKVAYIPPAKDIGPTPLHVQFILSVSNQHGGTVQGICFNITILPIDNQPPEVFSYDLKVEEGGFSQITASNIHITDKDTRQEQIKVILHKAPLHGIVQLDGSALQEGDPFTCLDLITLKVRYLHDGSETLQDEISFIATDGINSSEFILKIQVSPINDEPPFIKENLISTIICKEGAEVVISADYISATDADSDDMKLIFMIARQPRYGILLKAGSIIDQFTQRDVVEGLVHYRHTSGEIGLKVYVDIITLVVSDAEADKPQKKCCNNGPRLPILPNHESFPVYDLNVTILPVDNQPPSVIIGEIFVVDEGKSSALSVDHLSAMDPDTESNNLEFILVSPPRYGYIENVLPSPGFEKSNMGISIAAFLLKDIKSLNINYVQSRHQRIEPTTDQFTLYVTDGKLRSVEKPFYIVINPTNDEVPEFLARNITVVEGQMKKLDPTVINVSDMDVPQNHLYFTVIEQPRHGLIIEESYISGHMNRSQLIKRHQANSLPVIQDFSMDLLKNGMKMIYMHDDSENLADSFTIQLSDGKHEVRRTIFVNVIAVNDEKPVLTKHCDLVLSMGEQTLISSAILSAEDMDTPRTNISYVIDRCPEHGQLQMKVNKNWVTLHTGMKCTQEDIDMNLVRYVHTGTMGSKNEDTFTFHLWDGGNRSPEISVHIYVKDMEKGDIAVFVKMLNVSKGERVIITTNVLLATDGTDRPEELLYVLSSPPQYGQIEYVKHPGIPITSFSQMDVAAHTICYVHDFNSAATTDMFRFLVSNGLKTKHGIMNIALETVDRTLPTLSRNDGLRQVRGSMECISPATLQALDPDTSSQNITFQLVGLPQYGQLYLRGFPLQQDHFTQRDIDNLDIAYKHAGQDAQIDRFMFTVTDKANQGFIINGKIQTEPVPFTIQVDPVDSTAPRIVHLQCLTEVELLKNGQYGIYITSRHLKASDPDTDDERIVFHILGGPHYGYLENVTTGRFIRERFTQRDLNSKSVLYVINPSVDVFHDNIEFEVSDPVGNRALPQILELKWSQIELLQGMYEVCETEAVVSLKMTRKGYLLESAFVSLKVNEISAVLGKDISMNPSRLIQFDPGVSTKTWNIVIIQDGLEEDDEVFEVVLSSPVNAVLGSKTRAVVKIVDPRGGGCSSSKVSMIKTTGMGLLNCTVKPSRKPCAVFSFKETADKVREDVQEDVHTLQLPRKRLRLTGNRKTVKPSSVFRNKTDIIYQYHGIVSLRVEEDKPVLNETKKAKVLVKNRGQRKINAPSQNPDILQSGRTMLDSHVTTGGQDNAISFPKRCTPSLKGLLHFEDTVQKLFQCDGISWNPWITTKDSKAGKCPFGWTYHDGYCYILINDQKATWITAARACREQHFATLVDVFSKEQMEWLWNFSGRKSFWIGLNDRVRNGNWEWSKGEMVTFTNWKRGSPRSSHKGKNCVLAQKRGKWQAKDCKKGKGHYYICSKKL

Target ORF information:

RefSeq Version	XM_002939090.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis FRAS1 related extracellular matrix 1 (frem1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002939090.5

ORF Insert Sequence:

ATGAAGCCTT CTATTGTATA CCCAGTTTTG CTGTCAGTGT TGCTCACAAG GGGCCTCTGC 
GCCTCCTTTA TCAAAGTCAA TCACGGAATG AAAGTTATGA AAGGACAGAG TGCATTCCTC 
TCAGAAAGTG ACCTGCAGTT TTCCATCCCA AGGGAAAAGG ATGCCTGCAA AGTGGAAGTA 
GTTGTTAATG AACCCATTAC ACAGAGAGTT GGAAGGTTAA CACCACAGGT GTTTGACTGC 
CATTTCCTAG CCAATGAAGT TCAGTACACA CACAATGGCT GCCCTATCTT GGATGAAGAC 
GAGGTCATGC TCAGACTTTA CAGATTTACA GAAACTGAGA CTTTTACTGA AACCTTCCTG 
CTGAGAGTGA AGTTAATAGA GCCTGACTGT AATATCATAC AGCTGGGGCC TAAAAAGCTT 
GAAGTGCCAG AGTTTTATGG TCTGTCTGAC GTTATTGATA AGAACATACT GAGCTTTGAC 
TATGACAAAA GAATAAACAT TGAATGTACC ATCAGAATAT CGTCTTCTGA ATCTTTCCTT 
CCTGCACACG GTCAGCTTGT GACGGGGGAT CCTGTGAAAC AAGAACCACG AGGAGATCAG 
CCACACAGCT TCTTCCATGG ATCAAATCAA AAGCCAGGAC CTTTGTGCAA GAATGGAGGC 
TGCATACAAG GTTTAAAGAG GATCCAGAGC GTTATAAAAA TGAGTTGTGA AGATTTCCTT 
GTAAAGGGCA TCCGATACCA GCACTTGGAT CCTCCATCTC CAAATGTTGA TTATATCAGT 
ATCAGGTTGG ATCTTACAGA CTCAAGGAGC AAATCACTTT ACAAGTCAGA ACATGCTTGG 
ATTCCTGTTC TCATTCGGGA TGCTGTTCCA AATCAAATCC CCAAAGCCAA CTTCATGTCT 
ATGTTTATCT TAGAAGTAGA TCAGTTTGTT CTAACACCCA TCTCAACTGC AACACTGGAT 
GCCGAGGACA GCGAAACAGC CAAACATCTT CTGGTCTTCA ATATTACTTT ACCTCCAAGC 
AAAGGGTTCA TCACACACTT GTCTGACCAT ACCAAGCCCA TTACATCCTT TACATGGAAA 
GATCTCAATG AAATGCTCAT AGCATATCAG CCTCCGAACA GCAGCCACAC AGAGAGAAGA 
AATTTGGAGG TGGAGTTTGA GGTGCATGAT TTCTTTTTTG AAAAGAGTCC TCCAATTACA 
GTGCATATCT CTATACGTAC GGCTGACACC AATGCTCCTC GCGTTTCATG GAACATGGGT 
CTGGATTTAC TGGAGGGCCA GTCTCGACCA ATTACATGGA AACAGTTCCA AATTGTGGAT 
AATGACAACA TCAATGCAGT TCGTTTGGTC ATTGTAGATG GATTGCAGCA TGGACGTATT 
ACTCTGAGAG GTGGGAAAGG ATTTATATTC ACAGTTAAGG ACATCCAGGC TGGAGATGTT 
TGCTACCATC ATGATGACAG TGACACTACT AAGGATTTTA TAGTCTTCAG AATATTTGAT 
GGACGTCACA GCATACGACA CAAGTTTCCT ATTAACATCC TACCAAAAGA TGATAGCCCT 
CCCTTCCTCA TCACAAACAT TGTTATTGAG CTTAGTGAAG GTCAAGCTGT CCTTATCGAG 
GGATCTATGC TTTGTGCTTC TGATATGGAC TCTAGTGATG CATACATACG TTTTAATATC 
ACTAAGCCTT CTGTAGCTGG AGAGATTATA AAGAAGCCAG GACAAAATCT TATTGCTTAT 
CCTGTCACAA GCTTCCTGCA ACGAGACTTA TATAACGGTT TCATTTATTA TCGCCATCTT 
GGAGGAGAAG TATTTGAAGA TTCATTTGAT GTTGTTCTGT CTGATAGCCA TGATCCACCA 
AACTTTTCTG GTCCTCAGGC AGTAATCATT CATATTAGCC CCGTGGATGA CCAGCTGCCC 
ATAGAAGCAC CTGGAACAGT ACGACATCTG ATGGTAAAGG AGAATGAGGT TGTTTATATA 
ACAAAGAAAC AACTGCATTT CATAGACCCA GAGTCACCTG AAAGAGAACT TATTTACACA 
GTTGCTACTC CTCCTTGTAT GGTGCCAACC TACAGCAGCT TAGATGCGGG GAAGATAGTT 
CTTGTAGACA CTATACTTAA ACTTGGAAAG GATTATGCAG GAGACACAGC AAGAACATTT 
TCACAGCATG CTGTGAATCA CCTCAAAGTT GCTTACATAC CACCAGCTAA AGACATTGGG 
CCAACTCCTC TACATGTCCA GTTTATATTA TCAGTGAGTA ACCAACATGG AGGAACAGTT 
CAAGGCATCT GTTTCAATAT CACTATCCTT CCAATAGACA ATCAGCCTCC TGAGGTGTTT 
TCATATGATT TGAAAGTTGA AGAAGGTGGT TTCAGCCAAA TTACTGCAAG TAACATTCAC 
ATCACAGATA AGGACACCAG ACAAGAACAA ATTAAAGTTA TTTTACACAA AGCTCCACTG 
CATGGTATCG TTCAACTTGA TGGATCTGCA TTGCAGGAAG GTGACCCATT CACCTGCTTG 
GACCTCATCA CATTAAAAGT TAGGTACCTT CATGATGGTT CAGAGACCCT ACAAGATGAA 
ATCTCATTTA TTGCAACAGA TGGAATCAAT TCATCTGAGT TTATTTTAAA AATACAGGTT 
TCTCCTATTA ATGATGAACC TCCATTTATT AAAGAGAACC TAATTTCCAC CATTATTTGC 
AAAGAAGGGG CAGAGGTAGT CATATCAGCA GACTATATCT CAGCTACTGA TGCAGACAGT 
GATGACATGA AGCTGATATT TATGATCGCT CGCCAACCAC GTTATGGCAT TTTGCTAAAG 
GCTGGATCTA TCATTGATCA ATTCACCCAG AGAGATGTTG TTGAAGGACT GGTACATTAT 
AGACATACAA GTGGCGAAAT TGGCTTGAAG GTCTATGTTG ATATTATAAC ACTGGTGGTT 
TCTGATGCTG AAGCAGATAA ACCGCAGAAA AAGTGCTGCA ACAATGGTCC TCGTCTGCCG 
ATTTTGCCAA ACCATGAGTC TTTTCCTGTA TATGATCTCA ATGTTACAAT TCTGCCAGTG 
GACAACCAAC CCCCATCGGT TATAATAGGT GAAATATTTG TAGTCGATGA GGGCAAATCA 
TCTGCCCTCA GTGTAGATCA TTTAAGTGCC ATGGACCCTG ACACAGAAAG TAACAATCTG 
GAATTCATAC TGGTTTCTCC TCCTCGATAT GGTTACATTG AAAATGTCCT CCCATCTCCT 
GGATTTGAGA AGAGTAATAT GGGTATAAGT ATTGCTGCAT TTCTACTCAA GGACATTAAA 
TCTCTGAACA TCAATTATGT CCAGTCCCGC CATCAAAGAA TTGAACCAAC CACTGATCAG 
TTTACACTGT ATGTCACTGA TGGCAAACTG CGTTCTGTAG AAAAGCCATT TTACATCGTA 
ATCAATCCGA CAAATGATGA GGTTCCGGAA TTTTTAGCCA GAAACATAAC AGTTGTTGAA 
GGACAAATGA AGAAATTGGA TCCCACTGTA ATAAATGTGT CAGACATGGA TGTGCCACAG 
AACCACTTAT ACTTTACTGT TATAGAGCAG CCCCGACATG GACTGATTAT TGAGGAATCG 
TATATCAGTG GGCACATGAA TAGAAGCCAA TTAATAAAAA GGCATCAGGC AAACAGCCTG 
CCTGTGATAC AAGATTTTTC CATGGACCTG CTAAAAAATG GAATGAAGAT GATATACATG 
CATGATGACT CAGAAAACCT TGCTGACAGC TTCACTATCC AGCTATCTGA TGGAAAGCAT 
GAAGTCAGGA GAACTATTTT TGTGAATGTA ATTGCAGTTA ATGACGAAAA GCCTGTGCTA 
ACCAAGCACT GCGATTTGGT GCTAAGCATG GGGGAACAAA CTCTTATATC AAGTGCCATA 
CTGTCAGCAG AAGATATGGA CACACCCAGG ACAAACATTT CCTATGTTAT TGACAGATGT 
CCAGAGCATG GCCAGCTTCA GATGAAAGTC AATAAAAACT GGGTGACCTT GCATACTGGA 
ATGAAATGCA CTCAGGAGGA CATTGATATG AACCTGGTTA GATATGTGCA TACTGGAACA 
ATGGGCTCAA AAAATGAAGA CACGTTTACT TTCCACCTTT GGGATGGCGG AAACCGATCA 
CCTGAGATAT CTGTTCATAT TTATGTCAAG GATATGGAAA AAGGAGATAT TGCAGTTTTT 
GTGAAAATGC TTAATGTGTC CAAGGGAGAG AGAGTTATCA TAACAACAAA TGTCCTTCTC 
GCCACTGATG GGACCGACAG ACCAGAAGAG CTGCTGTATG TGTTATCCTC GCCACCCCAA 
TATGGGCAGA TTGAATATGT TAAGCATCCT GGAATCCCCA TAACAAGTTT CAGTCAGATG 
GATGTGGCTG CTCACACAAT CTGCTACGTC CACGATTTCA ATTCAGCAGC CACTACTGAT 
ATGTTTAGAT TCCTAGTCAG CAATGGGCTG AAAACGAAAC ATGGGATCAT GAACATCGCA 
CTGGAAACAG TCGATCGAAC TCTTCCAACA CTATCCAGGA ACGATGGTCT AAGACAAGTG 
AGAGGGTCTA TGGAGTGTAT TTCCCCGGCC ACCCTACAGG CACTGGACCC TGACACGTCA 
TCTCAGAACA TTACTTTTCA GTTGGTGGGT CTCCCTCAAT ATGGGCAATT ATACCTCAGA 
GGCTTTCCAC TACAGCAAGA TCACTTCACC CAAAGAGATA TTGACAATCT AGACATTGCC 
TATAAGCATG CTGGACAGGA TGCTCAAATA GACAGGTTTA TGTTTACTGT TACAGACAAA 
GCAAATCAAG GATTTATCAT TAATGGGAAG ATTCAGACGG AACCAGTTCC ATTCACAATA 
CAGGTAGATC CTGTGGACAG CACAGCTCCC AGAATTGTCC ATCTTCAATG CCTTACAGAA 
GTTGAGCTTC TGAAAAATGG CCAGTATGGA ATTTATATTA CTTCAAGACA CTTAAAGGCA 
TCTGATCCAG ACACAGATGA TGAAAGAATT GTCTTTCACA TATTAGGAGG GCCTCACTAT 
GGTTATTTAG AGAATGTTAC AACAGGCAGG TTTATAAGAG AAAGATTTAC ACAAAGGGAT 
TTAAACAGCA AATCAGTGCT CTACGTAATA AATCCATCAG TGGATGTTTT TCATGACAAC 
ATAGAGTTTG AAGTTTCAGA CCCCGTTGGA AACAGAGCTT TACCACAAAT TTTAGAGCTG 
AAATGGAGTC AAATTGAATT GCTCCAAGGC ATGTATGAGG TGTGTGAAAC CGAGGCAGTT 
GTGTCTTTGA AGATGACAAG AAAAGGTTAT CTCCTGGAGT CTGCTTTTGT TTCGCTCAAG 
GTGAATGAAA TAAGTGCTGT TCTTGGCAAA GACATTTCCA TGAATCCTTC CAGACTTATA 
CAGTTTGATC CAGGAGTGTC AACCAAGACT TGGAATATAG TAATTATCCA GGATGGATTA 
GAAGAAGATG ACGAGGTCTT TGAAGTAGTT CTGAGTTCTC CTGTGAATGC TGTTCTTGGC 
TCTAAGACAA GAGCTGTAGT GAAAATTGTG GACCCCAGAG GAGGTGGCTG CAGTTCCTCC 
AAAGTCAGCA TGATCAAAAC AACTGGAATG GGCTTACTAA ACTGTACCGT CAAACCCTCA 
CGCAAACCTT GTGCTGTTTT TAGTTTTAAA GAGACTGCAG ATAAGGTCAG GGAAGATGTC 
CAAGAAGATG TCCATACCTT ACAACTTCCG AGGAAGAGAC TAAGACTCAC AGGGAACAGA 
AAAACAGTTA AACCATCATC AGTATTTAGA AACAAAACAG ACATTATTTA TCAATATCAT 
GGGATTGTGT CTCTCCGAGT GGAAGAAGAT AAGCCAGTAT TAAATGAAAC AAAGAAAGCA 
AAAGTACTAG TGAAGAACCG TGGTCAGAGG AAGATAAATG CCCCCTCCCA AAATCCTGAC 
ATTCTTCAAT CAGGCAGAAC CATGTTGGAT TCTCATGTTA CTACTGGTGG TCAGGATAAC 
GCAATATCGT TTCCTAAAAG ATGCACACCA AGTTTAAAAG GACTCCTGCA TTTTGAGGAC 
ACAGTACAGA AACTGTTCCA GTGTGACGGC ATATCGTGGA ACCCCTGGAT CACTACTAAG 
GATTCTAAAG CAGGAAAATG CCCATTTGGC TGGACTTACC ATGATGGCTA CTGCTATATA 
CTGATCAATG ATCAAAAGGC CACCTGGATA ACAGCAGCCA GAGCATGTAG AGAACAGCAT 
TTTGCTACTC TGGTGGATGT ATTCAGCAAG GAACAAATGG AATGGCTTTG GAATTTCAGT 
GGCAGAAAAT CCTTTTGGAT AGGCCTCAAT GATAGAGTAC GTAATGGAAA CTGGGAGTGG 
AGCAAAGGGG AAATGGTCAC CTTCACCAAT TGGAAAAGAG GCTCTCCTCG ATCTTCACAC 
AAAGGGAAGA ATTGCGTTTT GGCACAAAAG AGAGGCAAAT GGCAGGCTAA AGACTGCAAA 
AAAGGCAAAG GCCATTACTA TATATGTTCA AAAAAGTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

frem1 ( XM_018097904.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017953393.1 Xenopus tropicalis FRAS1 related extracellular matrix 1 (frem1), transcript variant X2, mRNA.

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CloneID	OXa42096
Clone ID Related Accession (Same CDS sequence)	XM_018097904.1
Accession Version	XM_018097904.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	FRAS1-related extracellular matrix protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCCTT CTATTGTATA CCCAGTTTTG CTGTCAGTGT TGCTCACAAG GGGCCTCTGC 
GCCTCCTTTA TCAAAGTCAA TCACGGAATG AAAGTTATGA AAGGACAGAG TGCATTCCTC 
TCAGAAAGTG ACCTGCAGTT TTCCATCCCA AGGGAAAAGG ATGCCTGCAA AGTGGAAGTA 
GTTGTTAATG AACCCATTAC ACAGAGAGTT GGAAGGTTAA CACCACAGGT GTTTGACTGC 
CATTTCCTAG CCAATGAAGT TCAGTACACA CACAATGGCT GCCCTATCTT GGATGAAGAC 
GAGGTCATGC TCAGACTTTA CAGATTTACA GAAACTGAGA CTTTTACTGA AACCTTCCTG 
CTGAGAGTGA AGTTAATAGA GCCTGACTGT AATATCATAC AGCTGGGGCC TAAAAAGCTT 
GAAGTGCCAG AGTTTTATGG TCTGTCTGAC GTTATTGATA AGAACATACT GAGCTTTGAC 
TATGACAAAA GAATAAACAT TGAATGTACC ATCAGAATAT CGTCTTCTGA ATCTTTCCTT 
CCTGCACACG GTCAGCTTGT GACGGGGGAT CCTGTGAAAC AAGAACCACG AGGAGATCAG 
CCACACAGCT TCTTCCATGG ATCAAATCAA AAGCCAGGAC CTTTGTGCAA GAATGGAGGC 
TGCATACAAG GTTTAAAGAG GATCCAGAGC GTTATAAAAA TGAGTTGTGA AGATTTCCTT 
GTAAAGGGCA TCCGATACCA GCACTTGGAT CCTCCATCTC CAAATGTTGA TTATATCAGT 
ATCAGGTTGG ATCTTACAGA CTCAAGGAGC AAATCACTTT ACAAGTCAGA ACATGCTTGG 
ATTCCTGTTC TCATTCGGGA TGCTGTTCCA AATCAAATCC CCAAAGCCAA CTTCATGTCT 
ATGTTTATCT TAGAAGTAGA TCAGTTTGTT CTAACACCCA TCTCAACTGC AACACTGGAT 
GCCGAGGACA GCGAAACAGC CAAACATCTT CTGGTCTTCA ATATTACTTT ACCTCCAAGC 
AAAGGGTTCA TCACACACTT GTCTGACCAT ACCAAGCCCA TTACATCCTT TACATGGAAA 
GATCTCAATG AAATGCTCAT AGCATATCAG CCTCCGAACA GCAGCCACAC AGAGAGAAGA 
AATTTGGAGG TGGAGTTTGA GGTGCATGAT TTCTTTTTTG AAAAGAGTCC TCCAATTACA 
GTGCATATCT CTATACGTAC GGCTGACACC AATGCTCCTC GCGTTTCATG GAACATGGGT 
CTGGATTTAC TGGAGGGCCA GTCTCGACCA ATTACATGGA AACAGTTCCA AATTGTGGAT 
AATGACAACA TCAATGCAGT TCGTTTGGTC ATTGTAGATG GATTGCAGCA TGGACGTATT 
ACTCTGAGAG GTGGGAAAGG ATTTATATTC ACAGTTAAGG ACATCCAGGC TGGAGATGTT 
TGCTACCATC ATGATGACAG TGACACTACT AAGGATTTTA TAGTCTTCAG AATATTTGAT 
GGACGTCACA GCATACGACA CAAGTTTCCT ATTAACATCC TACCAAAAGA TGATAGCCCT 
CCCTTCCTCA TCACAAACAT TGTTATTGAG CTTAGTGAAG GTCAAGCTGT CCTTATCGAG 
GGATCTATGC TTTGTGCTTC TGATATGGAC TCTAGTGATG CATACATACG TTTTAATATC 
ACTAAGCCTT CTGTAGCTGG AGAGATTATA AAGAAGCCAG GACAAAATCT TATTGCTTAT 
CCTGTCACAA GCTTCCTGCA ACGAGACTTA TATAACGGTT TCATTTATTA TCGCCATCTT 
GGAGGAGAAG TATTTGAAGA TTCATTTGAT GTTGTTCTGT CTGATAGCCA TGATCCACCA 
AACTTTTCTG GTCCTCAGGC AGTAATCATT CATATTAGCC CCGTGGATGA CCAGCTGCCC 
ATAGAAGCAC CTGGAACAGT ACGACATCTG ATGGTAAAGG AGAATGAGGT TGTTTATATA 
ACAAAGAAAC AACTGCATTT CATAGACCCA GAGTCACCTG AAAGAGAACT TATTTACACA 
GTTGCTACTC CTCCTTGTAT GGTGCCAACC TACAGCAGCT TAGATGCGGG GAAGATAGTT 
CTTGTAGACA CTATACTTAA ACTTGGAAAG GATTATGCAG GAGACACAGC AAGAACATTT 
TCACAGCATG CTGTGAATCA CCTCAAAGTT GCTTACATAC CACCAGCTAA AGACATTGGG 
CCAACTCCTC TACATGTCCA GTTTATATTA TCAGTGAGTA ACCAACATGG AGGAACAGTT 
CAAGGCATCT GTTTCAATAT CACTATCCTT CCAATAGACA ATCAGCCTCC TGAGGTGTTT 
TCATATGATT TGAAAGTTGA AGAAGGTGGT TTCAGCCAAA TTACTGCAAG TAACATTCAC 
ATCACAGATA AGGACACCAG ACAAGAACAA ATTAAAGTTA TTTTACACAA AGCTCCACTG 
CATGGTATCG TTCAACTTGA TGGATCTGCA TTGCAGGAAG GTGACCCATT CACCTGCTTG 
GACCTCATCA CATTAAAAGT TAGGTACCTT CATGATGGTT CAGAGACCCT ACAAGATGAA 
ATCTCATTTA TTGCAACAGA TGGAATCAAT TCATCTGAGT TTATTTTAAA AATACAGGTT 
TCTCCTATTA ATGATGAACC TCCATTTATT AAAGAGAACC TAATTTCCAC CATTATTTGC 
AAAGAAGGGG CAGAGGTAGT CATATCAGCA GACTATATCT CAGCTACTGA TGCAGACAGT 
GATGACATGA AGCTGATATT TATGATCGCT CGCCAACCAC GTTATGGCAT TTTGCTAAAG 
GCTGGATCTA TCATTGATCA ATTCACCCAG AGAGATGTTG TTGAAGGACT GGTACATTAT 
AGACATACAA GTGGCGAAAT TGGCTTGAAG GTCTATGTTG ATATTATAAC ACTGGTGGTT 
TCTGATGCTG AAGCAGATAA ACCGCAGAAA AAGTGCTGCA ACAATGGTCC TCGTCTGCCG 
ATTTTGCCAA ACCATGAGTC TTTTCCTGTA TATGATCTCA ATGTTACAAT TCTGCCAGTG 
GACAACCAAC CCCCATCGGT TATAATAGGT GAAATATTTG TAGTCGATGA GGGCAAATCA 
TCTGCCCTCA GTGTAGATCA TTTAAGTGCC ATGGACCCTG ACACAGAAAG TAACAATCTG 
GAATTCATAC TGGTTTCTCC TCCTCGATAT GGTTACATTG AAAATGTCCT CCCATCTCCT 
GGATTTGAGA AGAGTAATAT GGGTATAAGT ATTGCTGCAT TTCTACTCAA GGACATTAAA 
TCTCTGAACA TCAATTATGT CCAGTCCCGC CATCAAAGAA TTGAACCAAC CACTGATCAG 
TTTACACTGT ATGTCACTGA TGGCAAACTG CGTTCTGTAG AAAAGCCATT TTACATCGTA 
ATCAATCCGA CAAATGATGA GGTTCCGGAA TTTTTAGCCA GAAACATAAC AGTTGTTGAA 
GGACAAATGA AGAAATTGGA TCCCACTGTA ATAAATGTGT CAGACATGGA TGTGCCACAG 
AACCACTTAT ACTTTACTGT TATAGAGCAG CCCCGACATG GACTGATTAT TGAGGAATCG 
TATATCAGTG GGCACATGAA TAGAAGCCAA TTAATAAAAA GGCATCAGGC AAACAGCCTG 
CCTGTGATAC AAGATTTTTC CATGGACCTG CTAAAAAATG GAATGAAGAT GATATACATG 
CATGATGACT CAGAAAACCT TGCTGACAGC TTCACTATCC AGCTATCTGA TGGAAAGCAT 
GAAGTCAGGA GAACTATTTT TGTGAATGTA ATTGCAGTTA ATGACGAAAA GCCTGTGCTA 
ACCAAGCACT GCGATTTGGT GCTAAGCATG GGGGAACAAA CTCTTATATC AAGTGCCATA 
CTGTCAGCAG AAGATATGGA CACACCCAGG ACAAACATTT CCTATGTTAT TGACAGATGT 
CCAGAGCATG GCCAGCTTCA GATGAAAGTC AATAAAAACT GGGTGACCTT GCATACTGGA 
ATGAAATGCA CTCAGGAGGA CATTGATATG AACCTGGTTA GATATGTGCA TACTGGAACA 
ATGGGCTCAA AAAATGAAGA CACGTTTACT TTCCACCTTT GGGATGGCGG AAACCGATCA 
CCTGAGATAT CTGTTCATAT TTATGTCAAG GATATGGAAA AAGGAGATAT TGCAGTTTTT 
GTGAAAATGC TTAATGTGTC CAAGGGAGAG AGAGTTATCA TAACAACAAA TGTCCTTCTC 
GCCACTGATG GGACCGACAG ACCAGAAGAG CTGCTGTATG TGTTATCCTC GCCACCCCAA 
TATGGGCAGA TTGAATATGT TAAGCATCCT GGAATCCCCA TAACAAGTTT CAGTCAGATG 
GATGTGGCTG CTCACACAAT CTGCTACGTC CACGATTTCA ATTCAGCAGC CACTACTGAT 
ATGTTTAGAT TCCTAGTCAG CAATGGGCTG AAAACGAAAC ATGGGATCAT GAACATCGCA 
CTGGAAACAG TCGATCGAAC TCTTCCAACA CTATCCAGGA ACGATGGTCT AAGACAAGTG 
AGAGGGTCTA TGGAGTGTAT TTCCCCGGCC ACCCTACAGG CACTGGACCC TGACACGTCA 
TCTCAGAACA TTACTTTTCA GTTGGTGGGT CTCCCTCAAT ATGGGCAATT ATACCTCAGA 
GGCTTTCCAC TACAGCAAGA TCACTTCACC CAAAGAGATA TTGACAATCT AGACATTGCC 
TATAAGCATG CTGGACAGGA TGCTCAAATA GACAGGTTTA TGTTTACTGT TACAGACAAA 
GCAAATCAAG GATTTATCAT TAATGGGAAG ATTCAGACGG AACCAGTTCC ATTCACAATA 
CAGGTAGATC CTGTGGACAG CACAGCTCCC AGAATTGTCC ATCTTCAATG CCTTACAGAA 
GTTGAGCTTC TGAAAAATGG CCAGTATGGA ATTTATATTA CTTCAAGACA CTTAAAGGCA 
TCTGATCCAG ACACAGATGA TGAAAGAATT GTCTTTCACA TATTAGGAGG GCCTCACTAT 
GGTTATTTAG AGAATGTTAC AACAGGCAGG TTTATAAGAG AAAGATTTAC ACAAAGGGAT 
TTAAACAGCA AATCAGTGCT CTACGTAATA AATCCATCAG TGGATGTTTT TCATGACAAC 
ATAGAGTTTG AAGTTTCAGA CCCCGTTGGA AACAGAGCTT TACCACAAAT TTTAGAGCTG 
AAATGGAGTC AAATTGAATT GCTCCAAGGC ATGTATGAGG TGTGTGAAAC CGAGGCAGTT 
GTGTCTTTGA AGATGACAAG AAAAGGTTAT CTCCTGGAGT CTGCTTTTGT TTCGCTCAAG 
GTGAATGAAA TAAGTGCTGT TCTTGGCAAA GACATTTCCA TGAATCCTTC CAGACTTATA 
CAGTTTGATC CAGGAGTGTC AACCAAGACT TGGAATATAG TAATTATCCA GGATGGATTA 
GAAGAAGATG ACGAGGTCTT TGAAGTAGTT CTGAGTTCTC CTGTGAATGC TGTTCTTGGC 
TCTAAGACAA GAGCTGTAGT GAAAATTGTG GACCCCAGAG GAGGTGGCTG CAGTTCCTCC 
AAAGTCAGCA TGATCAAAAC AACTGGAATG GGCTTACTAA ACTGTACCGT CAAACCCTCA 
CGCAAACCTT GTGCTGTTTT TAGTTTTAAA GAGACTGCAG ATAAGGTCAG GGAAGATGTC 
CAAGAAGATG TCCATACCTT ACAACTTCCG AGGAAGAGAC TAAGACTCAC AGGGAACAGA 
AAAACAGTTA AACCATCATC AGTATTTAGA AACAAAACAG ACATTATTTA TCAATATCAT 
GGGATTGTGT CTCTCCGAGT GGAAGAAGAT AAGCCAGTAT TAAATGAAAC AAAGAAAGCA 
AAAGTACTAG TGAAGAACCG TGGTCAGAGG AAGATAAATG CCCCCTCCCA AAATCCTGAC 
ATTCTTCAAT CAGGCAGAAC CATGTTGGAT TCTCATGTTA CTACTGGTGG TCAGGATAAC 
GCAATATCGT TTCCTAAAAG ATGCACACCA AGTTTAAAAG GACTCCTGCA TTTTGAGGAC 
ACAGTACAGA AACTGTTCCA GTGTGACGGC ATATCGTGGA ACCCCTGGAT CACTACTAAG 
GATTCTAAAG CAGGAAAATG CCCATTTGGC TGGACTTACC ATGATGGCTA CTGCTATATA 
CTGATCAATG ATCAAAAGGC CACCTGGATA ACAGCAGCCA GAGCATGTAG AGAACAGCAT 
TTTGCTACTC TGGTGGATGT ATTCAGCAAG GAACAAATGG AATGGCTTTG GAATTTCAGT 
GGCAGAAAAT CCTTTTGGAT AGGTGACTAC TTATATTATT ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017953393.1
CDS	608..6952
Translation	MKPSIVYPVLLSVLLTRGLCASFIKVNHGMKVMKGQSAFLSESDLQFSIPREKDACKVEVVVNEPITQRVGRLTPQVFDCHFLANEVQYTHNGCPILDEDEVMLRLYRFTETETFTETFLLRVKLIEPDCNIIQLGPKKLEVPEFYGLSDVIDKNILSFDYDKRINIECTIRISSSESFLPAHGQLVTGDPVKQEPRGDQPHSFFHGSNQKPGPLCKNGGCIQGLKRIQSVIKMSCEDFLVKGIRYQHLDPPSPNVDYISIRLDLTDSRSKSLYKSEHAWIPVLIRDAVPNQIPKANFMSMFILEVDQFVLTPISTATLDAEDSETAKHLLVFNITLPPSKGFITHLSDHTKPITSFTWKDLNEMLIAYQPPNSSHTERRNLEVEFEVHDFFFEKSPPITVHISIRTADTNAPRVSWNMGLDLLEGQSRPITWKQFQIVDNDNINAVRLVIVDGLQHGRITLRGGKGFIFTVKDIQAGDVCYHHDDSDTTKDFIVFRIFDGRHSIRHKFPINILPKDDSPPFLITNIVIELSEGQAVLIEGSMLCASDMDSSDAYIRFNITKPSVAGEIIKKPGQNLIAYPVTSFLQRDLYNGFIYYRHLGGEVFEDSFDVVLSDSHDPPNFSGPQAVIIHISPVDDQLPIEAPGTVRHLMVKENEVVYITKKQLHFIDPESPERELIYTVATPPCMVPTYSSLDAGKIVLVDTILKLGKDYAGDTARTFSQHAVNHLKVAYIPPAKDIGPTPLHVQFILSVSNQHGGTVQGICFNITILPIDNQPPEVFSYDLKVEEGGFSQITASNIHITDKDTRQEQIKVILHKAPLHGIVQLDGSALQEGDPFTCLDLITLKVRYLHDGSETLQDEISFIATDGINSSEFILKIQVSPINDEPPFIKENLISTIICKEGAEVVISADYISATDADSDDMKLIFMIARQPRYGILLKAGSIIDQFTQRDVVEGLVHYRHTSGEIGLKVYVDIITLVVSDAEADKPQKKCCNNGPRLPILPNHESFPVYDLNVTILPVDNQPPSVIIGEIFVVDEGKSSALSVDHLSAMDPDTESNNLEFILVSPPRYGYIENVLPSPGFEKSNMGISIAAFLLKDIKSLNINYVQSRHQRIEPTTDQFTLYVTDGKLRSVEKPFYIVINPTNDEVPEFLARNITVVEGQMKKLDPTVINVSDMDVPQNHLYFTVIEQPRHGLIIEESYISGHMNRSQLIKRHQANSLPVIQDFSMDLLKNGMKMIYMHDDSENLADSFTIQLSDGKHEVRRTIFVNVIAVNDEKPVLTKHCDLVLSMGEQTLISSAILSAEDMDTPRTNISYVIDRCPEHGQLQMKVNKNWVTLHTGMKCTQEDIDMNLVRYVHTGTMGSKNEDTFTFHLWDGGNRSPEISVHIYVKDMEKGDIAVFVKMLNVSKGERVIITTNVLLATDGTDRPEELLYVLSSPPQYGQIEYVKHPGIPITSFSQMDVAAHTICYVHDFNSAATTDMFRFLVSNGLKTKHGIMNIALETVDRTLPTLSRNDGLRQVRGSMECISPATLQALDPDTSSQNITFQLVGLPQYGQLYLRGFPLQQDHFTQRDIDNLDIAYKHAGQDAQIDRFMFTVTDKANQGFIINGKIQTEPVPFTIQVDPVDSTAPRIVHLQCLTEVELLKNGQYGIYITSRHLKASDPDTDDERIVFHILGGPHYGYLENVTTGRFIRERFTQRDLNSKSVLYVINPSVDVFHDNIEFEVSDPVGNRALPQILELKWSQIELLQGMYEVCETEAVVSLKMTRKGYLLESAFVSLKVNEISAVLGKDISMNPSRLIQFDPGVSTKTWNIVIIQDGLEEDDEVFEVVLSSPVNAVLGSKTRAVVKIVDPRGGGCSSSKVSMIKTTGMGLLNCTVKPSRKPCAVFSFKETADKVREDVQEDVHTLQLPRKRLRLTGNRKTVKPSSVFRNKTDIIYQYHGIVSLRVEEDKPVLNETKKAKVLVKNRGQRKINAPSQNPDILQSGRTMLDSHVTTGGQDNAISFPKRCTPSLKGLLHFEDTVQKLFQCDGISWNPWITTKDSKAGKCPFGWTYHDGYCYILINDQKATWITAARACREQHFATLVDVFSKEQMEWLWNFSGRKSFWIGDYLYYY

Target ORF information:

RefSeq Version	XM_018097904.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis FRAS1 related extracellular matrix 1 (frem1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018097904.1

ORF Insert Sequence:

ATGAAGCCTT CTATTGTATA CCCAGTTTTG CTGTCAGTGT TGCTCACAAG GGGCCTCTGC 
GCCTCCTTTA TCAAAGTCAA TCACGGAATG AAAGTTATGA AAGGACAGAG TGCATTCCTC 
TCAGAAAGTG ACCTGCAGTT TTCCATCCCA AGGGAAAAGG ATGCCTGCAA AGTGGAAGTA 
GTTGTTAATG AACCCATTAC ACAGAGAGTT GGAAGGTTAA CACCACAGGT GTTTGACTGC 
CATTTCCTAG CCAATGAAGT TCAGTACACA CACAATGGCT GCCCTATCTT GGATGAAGAC 
GAGGTCATGC TCAGACTTTA CAGATTTACA GAAACTGAGA CTTTTACTGA AACCTTCCTG 
CTGAGAGTGA AGTTAATAGA GCCTGACTGT AATATCATAC AGCTGGGGCC TAAAAAGCTT 
GAAGTGCCAG AGTTTTATGG TCTGTCTGAC GTTATTGATA AGAACATACT GAGCTTTGAC 
TATGACAAAA GAATAAACAT TGAATGTACC ATCAGAATAT CGTCTTCTGA ATCTTTCCTT 
CCTGCACACG GTCAGCTTGT GACGGGGGAT CCTGTGAAAC AAGAACCACG AGGAGATCAG 
CCACACAGCT TCTTCCATGG ATCAAATCAA AAGCCAGGAC CTTTGTGCAA GAATGGAGGC 
TGCATACAAG GTTTAAAGAG GATCCAGAGC GTTATAAAAA TGAGTTGTGA AGATTTCCTT 
GTAAAGGGCA TCCGATACCA GCACTTGGAT CCTCCATCTC CAAATGTTGA TTATATCAGT 
ATCAGGTTGG ATCTTACAGA CTCAAGGAGC AAATCACTTT ACAAGTCAGA ACATGCTTGG 
ATTCCTGTTC TCATTCGGGA TGCTGTTCCA AATCAAATCC CCAAAGCCAA CTTCATGTCT 
ATGTTTATCT TAGAAGTAGA TCAGTTTGTT CTAACACCCA TCTCAACTGC AACACTGGAT 
GCCGAGGACA GCGAAACAGC CAAACATCTT CTGGTCTTCA ATATTACTTT ACCTCCAAGC 
AAAGGGTTCA TCACACACTT GTCTGACCAT ACCAAGCCCA TTACATCCTT TACATGGAAA 
GATCTCAATG AAATGCTCAT AGCATATCAG CCTCCGAACA GCAGCCACAC AGAGAGAAGA 
AATTTGGAGG TGGAGTTTGA GGTGCATGAT TTCTTTTTTG AAAAGAGTCC TCCAATTACA 
GTGCATATCT CTATACGTAC GGCTGACACC AATGCTCCTC GCGTTTCATG GAACATGGGT 
CTGGATTTAC TGGAGGGCCA GTCTCGACCA ATTACATGGA AACAGTTCCA AATTGTGGAT 
AATGACAACA TCAATGCAGT TCGTTTGGTC ATTGTAGATG GATTGCAGCA TGGACGTATT 
ACTCTGAGAG GTGGGAAAGG ATTTATATTC ACAGTTAAGG ACATCCAGGC TGGAGATGTT 
TGCTACCATC ATGATGACAG TGACACTACT AAGGATTTTA TAGTCTTCAG AATATTTGAT 
GGACGTCACA GCATACGACA CAAGTTTCCT ATTAACATCC TACCAAAAGA TGATAGCCCT 
CCCTTCCTCA TCACAAACAT TGTTATTGAG CTTAGTGAAG GTCAAGCTGT CCTTATCGAG 
GGATCTATGC TTTGTGCTTC TGATATGGAC TCTAGTGATG CATACATACG TTTTAATATC 
ACTAAGCCTT CTGTAGCTGG AGAGATTATA AAGAAGCCAG GACAAAATCT TATTGCTTAT 
CCTGTCACAA GCTTCCTGCA ACGAGACTTA TATAACGGTT TCATTTATTA TCGCCATCTT 
GGAGGAGAAG TATTTGAAGA TTCATTTGAT GTTGTTCTGT CTGATAGCCA TGATCCACCA 
AACTTTTCTG GTCCTCAGGC AGTAATCATT CATATTAGCC CCGTGGATGA CCAGCTGCCC 
ATAGAAGCAC CTGGAACAGT ACGACATCTG ATGGTAAAGG AGAATGAGGT TGTTTATATA 
ACAAAGAAAC AACTGCATTT CATAGACCCA GAGTCACCTG AAAGAGAACT TATTTACACA 
GTTGCTACTC CTCCTTGTAT GGTGCCAACC TACAGCAGCT TAGATGCGGG GAAGATAGTT 
CTTGTAGACA CTATACTTAA ACTTGGAAAG GATTATGCAG GAGACACAGC AAGAACATTT 
TCACAGCATG CTGTGAATCA CCTCAAAGTT GCTTACATAC CACCAGCTAA AGACATTGGG 
CCAACTCCTC TACATGTCCA GTTTATATTA TCAGTGAGTA ACCAACATGG AGGAACAGTT 
CAAGGCATCT GTTTCAATAT CACTATCCTT CCAATAGACA ATCAGCCTCC TGAGGTGTTT 
TCATATGATT TGAAAGTTGA AGAAGGTGGT TTCAGCCAAA TTACTGCAAG TAACATTCAC 
ATCACAGATA AGGACACCAG ACAAGAACAA ATTAAAGTTA TTTTACACAA AGCTCCACTG 
CATGGTATCG TTCAACTTGA TGGATCTGCA TTGCAGGAAG GTGACCCATT CACCTGCTTG 
GACCTCATCA CATTAAAAGT TAGGTACCTT CATGATGGTT CAGAGACCCT ACAAGATGAA 
ATCTCATTTA TTGCAACAGA TGGAATCAAT TCATCTGAGT TTATTTTAAA AATACAGGTT 
TCTCCTATTA ATGATGAACC TCCATTTATT AAAGAGAACC TAATTTCCAC CATTATTTGC 
AAAGAAGGGG CAGAGGTAGT CATATCAGCA GACTATATCT CAGCTACTGA TGCAGACAGT 
GATGACATGA AGCTGATATT TATGATCGCT CGCCAACCAC GTTATGGCAT TTTGCTAAAG 
GCTGGATCTA TCATTGATCA ATTCACCCAG AGAGATGTTG TTGAAGGACT GGTACATTAT 
AGACATACAA GTGGCGAAAT TGGCTTGAAG GTCTATGTTG ATATTATAAC ACTGGTGGTT 
TCTGATGCTG AAGCAGATAA ACCGCAGAAA AAGTGCTGCA ACAATGGTCC TCGTCTGCCG 
ATTTTGCCAA ACCATGAGTC TTTTCCTGTA TATGATCTCA ATGTTACAAT TCTGCCAGTG 
GACAACCAAC CCCCATCGGT TATAATAGGT GAAATATTTG TAGTCGATGA GGGCAAATCA 
TCTGCCCTCA GTGTAGATCA TTTAAGTGCC ATGGACCCTG ACACAGAAAG TAACAATCTG 
GAATTCATAC TGGTTTCTCC TCCTCGATAT GGTTACATTG AAAATGTCCT CCCATCTCCT 
GGATTTGAGA AGAGTAATAT GGGTATAAGT ATTGCTGCAT TTCTACTCAA GGACATTAAA 
TCTCTGAACA TCAATTATGT CCAGTCCCGC CATCAAAGAA TTGAACCAAC CACTGATCAG 
TTTACACTGT ATGTCACTGA TGGCAAACTG CGTTCTGTAG AAAAGCCATT TTACATCGTA 
ATCAATCCGA CAAATGATGA GGTTCCGGAA TTTTTAGCCA GAAACATAAC AGTTGTTGAA 
GGACAAATGA AGAAATTGGA TCCCACTGTA ATAAATGTGT CAGACATGGA TGTGCCACAG 
AACCACTTAT ACTTTACTGT TATAGAGCAG CCCCGACATG GACTGATTAT TGAGGAATCG 
TATATCAGTG GGCACATGAA TAGAAGCCAA TTAATAAAAA GGCATCAGGC AAACAGCCTG 
CCTGTGATAC AAGATTTTTC CATGGACCTG CTAAAAAATG GAATGAAGAT GATATACATG 
CATGATGACT CAGAAAACCT TGCTGACAGC TTCACTATCC AGCTATCTGA TGGAAAGCAT 
GAAGTCAGGA GAACTATTTT TGTGAATGTA ATTGCAGTTA ATGACGAAAA GCCTGTGCTA 
ACCAAGCACT GCGATTTGGT GCTAAGCATG GGGGAACAAA CTCTTATATC AAGTGCCATA 
CTGTCAGCAG AAGATATGGA CACACCCAGG ACAAACATTT CCTATGTTAT TGACAGATGT 
CCAGAGCATG GCCAGCTTCA GATGAAAGTC AATAAAAACT GGGTGACCTT GCATACTGGA 
ATGAAATGCA CTCAGGAGGA CATTGATATG AACCTGGTTA GATATGTGCA TACTGGAACA 
ATGGGCTCAA AAAATGAAGA CACGTTTACT TTCCACCTTT GGGATGGCGG AAACCGATCA 
CCTGAGATAT CTGTTCATAT TTATGTCAAG GATATGGAAA AAGGAGATAT TGCAGTTTTT 
GTGAAAATGC TTAATGTGTC CAAGGGAGAG AGAGTTATCA TAACAACAAA TGTCCTTCTC 
GCCACTGATG GGACCGACAG ACCAGAAGAG CTGCTGTATG TGTTATCCTC GCCACCCCAA 
TATGGGCAGA TTGAATATGT TAAGCATCCT GGAATCCCCA TAACAAGTTT CAGTCAGATG 
GATGTGGCTG CTCACACAAT CTGCTACGTC CACGATTTCA ATTCAGCAGC CACTACTGAT 
ATGTTTAGAT TCCTAGTCAG CAATGGGCTG AAAACGAAAC ATGGGATCAT GAACATCGCA 
CTGGAAACAG TCGATCGAAC TCTTCCAACA CTATCCAGGA ACGATGGTCT AAGACAAGTG 
AGAGGGTCTA TGGAGTGTAT TTCCCCGGCC ACCCTACAGG CACTGGACCC TGACACGTCA 
TCTCAGAACA TTACTTTTCA GTTGGTGGGT CTCCCTCAAT ATGGGCAATT ATACCTCAGA 
GGCTTTCCAC TACAGCAAGA TCACTTCACC CAAAGAGATA TTGACAATCT AGACATTGCC 
TATAAGCATG CTGGACAGGA TGCTCAAATA GACAGGTTTA TGTTTACTGT TACAGACAAA 
GCAAATCAAG GATTTATCAT TAATGGGAAG ATTCAGACGG AACCAGTTCC ATTCACAATA 
CAGGTAGATC CTGTGGACAG CACAGCTCCC AGAATTGTCC ATCTTCAATG CCTTACAGAA 
GTTGAGCTTC TGAAAAATGG CCAGTATGGA ATTTATATTA CTTCAAGACA CTTAAAGGCA 
TCTGATCCAG ACACAGATGA TGAAAGAATT GTCTTTCACA TATTAGGAGG GCCTCACTAT 
GGTTATTTAG AGAATGTTAC AACAGGCAGG TTTATAAGAG AAAGATTTAC ACAAAGGGAT 
TTAAACAGCA AATCAGTGCT CTACGTAATA AATCCATCAG TGGATGTTTT TCATGACAAC 
ATAGAGTTTG AAGTTTCAGA CCCCGTTGGA AACAGAGCTT TACCACAAAT TTTAGAGCTG 
AAATGGAGTC AAATTGAATT GCTCCAAGGC ATGTATGAGG TGTGTGAAAC CGAGGCAGTT 
GTGTCTTTGA AGATGACAAG AAAAGGTTAT CTCCTGGAGT CTGCTTTTGT TTCGCTCAAG 
GTGAATGAAA TAAGTGCTGT TCTTGGCAAA GACATTTCCA TGAATCCTTC CAGACTTATA 
CAGTTTGATC CAGGAGTGTC AACCAAGACT TGGAATATAG TAATTATCCA GGATGGATTA 
GAAGAAGATG ACGAGGTCTT TGAAGTAGTT CTGAGTTCTC CTGTGAATGC TGTTCTTGGC 
TCTAAGACAA GAGCTGTAGT GAAAATTGTG GACCCCAGAG GAGGTGGCTG CAGTTCCTCC 
AAAGTCAGCA TGATCAAAAC AACTGGAATG GGCTTACTAA ACTGTACCGT CAAACCCTCA 
CGCAAACCTT GTGCTGTTTT TAGTTTTAAA GAGACTGCAG ATAAGGTCAG GGAAGATGTC 
CAAGAAGATG TCCATACCTT ACAACTTCCG AGGAAGAGAC TAAGACTCAC AGGGAACAGA 
AAAACAGTTA AACCATCATC AGTATTTAGA AACAAAACAG ACATTATTTA TCAATATCAT 
GGGATTGTGT CTCTCCGAGT GGAAGAAGAT AAGCCAGTAT TAAATGAAAC AAAGAAAGCA 
AAAGTACTAG TGAAGAACCG TGGTCAGAGG AAGATAAATG CCCCCTCCCA AAATCCTGAC 
ATTCTTCAAT CAGGCAGAAC CATGTTGGAT TCTCATGTTA CTACTGGTGG TCAGGATAAC 
GCAATATCGT TTCCTAAAAG ATGCACACCA AGTTTAAAAG GACTCCTGCA TTTTGAGGAC 
ACAGTACAGA AACTGTTCCA GTGTGACGGC ATATCGTGGA ACCCCTGGAT CACTACTAAG 
GATTCTAAAG CAGGAAAATG CCCATTTGGC TGGACTTACC ATGATGGCTA CTGCTATATA 
CTGATCAATG ATCAAAAGGC CACCTGGATA ACAGCAGCCA GAGCATGTAG AGAACAGCAT 
TTTGCTACTC TGGTGGATGT ATTCAGCAAG GAACAAATGG AATGGCTTTG GAATTTCAGT 
GGCAGAAAAT CCTTTTGGAT AGGTGACTAC TTATATTATT ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

frem1 ( XM_031890521.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746381.1 Xenopus tropicalis FRAS1 related extracellular matrix 1 (frem1), transcript variant X2, mRNA.

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CloneID	OXa48723
Clone ID Related Accession (Same CDS sequence)	XM_031890521.1
Accession Version	XM_031890521.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	FRAS1-related extracellular matrix protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCCTT CTATTGTATA CCCAGTTTTG CTGTCAGTGT TGCTCACAAG GGGCCTCTGC 
GCCTCCTTTA TCAAAGTCAA TCACGGAATG AAAGTTATGA AAGGACAGAG TGCATTCCTC 
TCAGAAAGTG ACCTGCAGTT TTCCATCCCA AGGGAAAAGG ATGCCTGCAA AGTGGAAGTA 
GTTGTTAATG AACCCATTAC ACAGAGAGTT GGAAGGTTAA CACCACAGGT GTTTGACTGC 
CATTTCCTAG CCAATGAAGT TCAGTACACA CACAATGGCT GCCCTATCTT GGATGAAGAC 
GAGGTCATGC TCAGACTTTA CAGATTTACA GAAACTGAGA CTTTTACTGA AACCTTCCTG 
CTGAGAGTGA AGTTAATAGA GCCTGACTGT AATATCATAC AGCTGGGGCC TAAAAAGCTT 
GAAGTGCCAG AGTTTTATGG TCTGTCTGAC GTTATTGATA AGAACATACT GAGCTTTGAC 
TATGACAAAA GAATAAACAT TGAATGTACC ATCAGAATAT CGTCTTCTGA ATCTTTCCTT 
CCTGCACACG GTCAGCTTGT GACGGGGGAT CCTGTGAAAC AAGAACCACG AGGAGATCAG 
CCACACAGCT TCTTCCATGG ATCAAATCAA AAGCCAGGAC CTTTGTGCAA GAATGGAGGC 
TGCATACAAG GTTTAAAGAG GATCCAGAGC GTTATAAAAA TGAGTTGTGA AGATTTCCTT 
GTAAAGGGCA TCCGATACCA GCACTTGGAT CCTCCATCTC CAAATGTTGA TTATATCAGT 
ATCAGGTTGG ATCTTACAGA CTCAAGGAGC AAATCACTTT ACAAGTCAGA ACATGCTTGG 
ATTCCTGTTC TCATTCGGGA TGCTGTTCCA AATCAAATCC CCAAAGCCAA CTTCATGTCT 
ATGTTTATCT TAGAAGTAGA TCAGTTTGTT CTAACACCCA TCTCAACTGC AACACTGGAT 
GCCGAGGACA GCGAAACAGC CAAACATCTT CTGGTCTTCA ATATTACTTT ACCTCCAAGC 
AAAGGGTTCA TCACACACTT GTCTGACCAT ACCAAGCCCA TTACATCCTT TACATGGAAA 
GATCTCAATG AAATGCTCAT AGCATATCAG CCTCCGAACA GCAGCCACAC AGAGAGAAGA 
AATTTGGAGG TGGAGTTTGA GGTGCATGAT TTCTTTTTTG AAAAGAGTCC TCCAATTACA 
GTGCATATCT CTATACGTAC GGCTGACACC AATGCTCCTC GCGTTTCATG GAACATGGGT 
CTGGATTTAC TGGAGGGCCA GTCTCGACCA ATTACATGGA AACAGTTCCA AATTGTGGAT 
AATGACAACA TCAATGCAGT TCGTTTGGTC ATTGTAGATG GATTGCAGCA TGGACGTATT 
ACTCTGAGAG GTGGGAAAGG ATTTATATTC ACAGTTAAGG ACATCCAGGC TGGAGATGTT 
TGCTACCATC ATGATGACAG TGACACTACT AAGGATTTTA TAGTCTTCAG AATATTTGAT 
GGACGTCACA GCATACGACA CAAGTTTCCT ATTAACATCC TACCAAAAGA TGATAGCCCT 
CCCTTCCTCA TCACAAACAT TGTTATTGAG CTTAGTGAAG GTCAAGCTGT CCTTATCGAG 
GGATCTATGC TTTGTGCTTC TGATATGGAC TCTAGTGATG CATACATACG TTTTAATATC 
ACTAAGCCTT CTGTAGCTGG AGAGATTATA AAGAAGCCAG GACAAAATCT TATTGCTTAT 
CCTGTCACAA GCTTCCTGCA ACGAGACTTA TATAACGGTT TCATTTATTA TCGCCATCTT 
GGAGGAGAAG TATTTGAAGA TTCATTTGAT GTTGTTCTGT CTGATAGCCA TGATCCACCA 
AACTTTTCTG GTCCTCAGGC AGTAATCATT CATATTAGCC CCGTGGATGA CCAGCTGCCC 
ATAGAAGCAC CTGGAACAGT ACGACATCTG ATGGTAAAGG AGAATGAGGT TGTTTATATA 
ACAAAGAAAC AACTGCATTT CATAGACCCA GAGTCACCTG AAAGAGAACT TATTTACACA 
GTTGCTACTC CTCCTTGTAT GGTGCCAACC TACAGCTTAG ATGCGGGGAA GATAGTTCTT 
GTAGACACTA TACTTAAACT TGGAAAGGAT TATGCAGGAG ACACAGCAAG AACATTTTCA 
CAGCATGCTG TGAATCACCT CAAAGTTGCT TACATACCAC CAGCTAAAGA CATTGGGCCA 
ACTCCTCTAC ATGTCCAGTT TATATTATCA GTGAGTAACC AACATGGAGG AACAGTTCAA 
GGCATCTGTT TCAATATCAC TATCCTTCCA ATAGACAATC AGCCTCCTGA GGTGTTTTCA 
TATGATTTGA AAGTTGAAGA AGGTGGTTTC AGCCAAATTA CTGCAAGTAA CATTCACATC 
ACAGATAAGG ACACCAGACA AGAACAAATT AAAGTTATTT TACACAAAGC TCCACTGCAT 
GGTATCGTTC AACTTGATGG ATCTGCATTG CAGGAAGGTG ACCCATTCAC CTGCTTGGAC 
CTCATCACAT TAAAAGTTAG GTACCTTCAT GATGGTTCAG AGACCCTACA AGATGAAATC 
TCATTTATTG CAACAGATGG AATCAATTCA TCTGAGTTTA TTTTAAAAAT ACAGGTTTCT 
CCTATTAATG ATGAACCTCC ATTTATTAAA GAGAACCTAA TTTCCACCAT TATTTGCAAA 
GAAGGGGCAG AGGTAGTCAT ATCAGCAGAC TATATCTCAG CTACTGATGC AGACAGTGAT 
GACATGAAGC TGATATTTAT GATCGCTCGC CAACCACGTT ATGGCATTTT GCTAAAGGCT 
GGATCTATCA TTGATCAATT CACCCAGAGA GATGTTGTTG AAGGACTGGT ACATTATAGA 
CATACAAGTG GCGAAATTGG CTTGAAGGTC TATGTTGATA TTATAACACT GGTGGTTTCT 
GATGCTGAAG CAGATAAACC GCAGAAAAAG TGCTGCAACA ATGGTCCTCG TCTGCCGATT 
TTGCCAAACC ATGAGTCTTT TCCTGTATAT GATCTCAATG TTACAATTCT GCCAGTGGAC 
AACCAACCCC CATCGGTTAT AATAGGTGAA ATATTTGTAG TCGATGAGGG CAAATCATCT 
GCCCTCAGTG TAGATCATTT AAGTGCCATG GACCCTGACA CAGAAAGTAA CAATCTGGAA 
TTCATACTGG TTTCTCCTCC TCGATATGGT TACATTGAAA ATGTCCTCCC ATCTCCTGGA 
TTTGAGAAGA GTAATATGGG TATAAGTATT GCTGCATTTC TACTCAAGGA CATTAAATCT 
CTGAACATCA ATTATGTCCA GTCCCGCCAT CAAAGAATTG AACCAACCAC TGATCAGTTT 
ACACTGTATG TCACTGATGG CAAACTGCGT TCTGTAGAAA AGCCATTTTA CATCGTAATC 
AATCCGACAA ATGATGAGGT TCCGGAATTT TTAGCCAGAA ACATAACAGT TGTTGAAGGA 
CAAATGAAGA AATTGGATCC CACTGTAATA AATGTGTCAG ACATGGATGT GCCACAGAAC 
CACTTATACT TTACTGTTAT AGAGCAGCCC CGACATGGAC TGATTATTGA GGAATCGTAT 
ATCAGTGGGC ACATGAATAG AAGCCAATTA ATAAAAAGGC ATCAGGCAAA CAGCCTGCCT 
GTGATACAAG ATTTTTCCAT GGACCTGCTA AAAAATGGAA TGAAGATGAT ATACATGCAT 
GATGACTCAG AAAACCTTGC TGACAGCTTC ACTATCCAGC TATCTGATGG AAAGCATGAA 
GTCAGGAGAA CTATTTTTGT GAATGTAATT GCAGTTAATG ACGAAAAGCC TGTGCTAACC 
AAGCACTGCG ATTTGGTGCT AAGCATGGGG GAACAAACTC TTATATCAAG TGCCATACTG 
TCAGCAGAAG ATATGGACAC ACCCAGGACA AACATTTCCT ATGTTATTGA CAGATGTCCA 
GAGCATGGCC AGCTTCAGAT GAAAGTCAAT AAAAACTGGG TGACCTTGCA TACTGGAATG 
AAATGCACTC AGGAGGACAT TGATATGAAC CTGGTTAGAT ATGTGCATAC TGGAACAATG 
GGCTCAAAAA ATGAAGACAC GTTTACTTTC CACCTTTGGG ATGGCGGAAA CCGATCACCT 
GAGATATCTG TTCATATTTA TGTCAAGGAT ATGGAAAAAG GAGATATTGC AGTTTTTGTG 
AAAATGCTTA ATGTGTCCAA GGGAGAGAGA GTTATCATAA CAACAAATGT CCTTCTCGCC 
ACTGATGGGA CCGACAGACC AGAAGAGCTG CTGTATGTGT TATCCTCGCC ACCCCAATAT 
GGGCAGATTG AATATGTTAA GCATCCTGGA ATCCCCATAA CAAGTTTCAG TCAGATGGAT 
GTGGCTGCTC ACACAATCTG CTACGTCCAC GATTTCAATT CAGCAGCCAC TACTGATATG 
TTTAGATTCC TAGTCAGCAA TGGGCTGAAA ACGAAACATG GGATCATGAA CATCGCACTG 
GAAACAGTCG ATCGAACTCT TCCAACACTA TCCAGGAACG ATGGTCTAAG ACAAGTGAGA 
GGGTCTATGG AGTGTATTTC CCCGGCCACC CTACAGGCAC TGGACCCTGA CACGTCATCT 
CAGAACATTA CTTTTCAGTT GGTGGGTCTC CCTCAATATG GGCAATTATA CCTCAGAGGC 
TTTCCACTAC AGCAAGATCA CTTCACCCAA AGAGATATTG ACAATCTAGA CATTGCCTAT 
AAGCATGCTG GACAGGATGC TCAAATAGAC AGGTTTATGT TTACTGTTAC AGACAAAGCA 
AATCAAGGAT TTATCATTAA TGGGAAGATT CAGACGGAAC CAGTTCCATT CACAATACAG 
GTAGATCCTG TGGACAGCAC AGCTCCCAGA ATTGTCCATC TTCAATGCCT TACAGAAGTT 
GAGCTTCTGA AAAATGGCCA GTATGGAATT TATATTACTT CAAGACACTT AAAGGCATCT 
GATCCAGACA CAGATGATGA AAGAATTGTC TTTCACATAT TAGGAGGGCC TCACTATGGT 
TATTTAGAGA ATGTTACAAC AGGCAGGTTT ATAAGAGAAA GATTTACACA AAGGGATTTA 
AACAGCAAAT CAGTGCTCTA CGTAATAAAT CCATCAGTGG ATGTTTTTCA TGACAACATA 
GAGTTTGAAG TTTCAGACCC CGTTGGAAAC AGAGCTTTAC CACAAATTTT AGAGCTGAAA 
TGGAGTCAAA TTGAATTGCT CCAAGGCATG TATGAGGTGT GTGAAACCGA GGCAGTTGTG 
TCTTTGAAGA TGACAAGAAA AGGTTATCTC CTGGAGTCTG CTTTTGTTTC GCTCAAGGTG 
AATGAAATAA GTGCTGTTCT TGGCAAAGAC ATTTCCATGA ATCCTTCCAG ACTTATACAG 
TTTGATCCAG GAGTGTCAAC CAAGACTTGG AATATAGTAA TTATCCAGGA TGGATTAGAA 
GAAGATGACG AGGTCTTTGA AGTAGTTCTG AGTTCTCCTG TGAATGCTGT TCTTGGCTCT 
AAGACAAGAG CTGTAGTGAA AATTGTGGAC CCCAGAGGAG GTGGCTGCAG TTCCTCCAAA 
GTCAGCATGA TCAAAACAAC TGGAATGGGC TTACTAAACT GTACCGTCAA ACCCTCACGC 
AAACCTTGTG CTGTTTTTAG TTTTAAAGAG ACTGCAGATA AGGTCAGGGA AGATGTCCAA 
GAAGATGTCC ATACCTTACA ACTTCCGAGG AAGAGACTAA GACTCACAGG GAACAGAAAA 
ACAGTTAAAC CATCATCAGT ATTTAGAAAC AAAACAGACA TTATTTATCA ATATCATGGG 
ATTGTGTCTC TCCGAGTGGA AGAAGATAAG CCAGTATTAA ATGAAACAAA GAAAGCAAAA 
GTACTAGTGA AGAACCGTGG TCAGAGGAAG ATAAATGCCC CCTCCCAAAA TCCTGACATT 
CTTCAATCAG GCAGAACCAT GTTGGATTCT CATGTTACTA CTGGTGGTCA GGATAACGCA 
ATATCGTTTC CTAAAAGATG CACACCAAGT TTAAAAGGAC TCCTGCATTT TGAGGACACA 
GTACAGAAAC TGTTCCAGTG TGACGGCATA TCGTGGAACC CCTGGATCAC TACTAAGGAT 
TCTAAAGCAG GAAAATGCCC ATTTGGCTGG ACTTACCATG ATGGCTACTG CTATATACTG 
ATCAATGATC AAAAGGCCAC CTGGATAACA GCAGCCAGAG CATGTAGAGA ACAGCATTTT 
GCTACTCTGG TGGATGTATT CAGCAAGGAA CAAATGGAAT GGCTTTGGAA TTTCAGTGGC 
AGAAAATCCT TTTGGATAGG CCTCAATGAT AGAGTACGTA ATGGAAACTG GGAGTGGAGC 
AAAGGGGAAA TGGTCACCTT CACCAATTGG AAAAGAGGCT CTCCTCGATC TTCACACAAA 
GGGAAGAATT GCGTTTTGGC ACAAAAGAGA GGCAAATGGC AGGCTAAAGA CTGCAAAAAA 
GGCAAAGGCC ATTACTATAT ATGTTCAAAA AAGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746381.1
CDS	613..7131
Translation	MKPSIVYPVLLSVLLTRGLCASFIKVNHGMKVMKGQSAFLSESDLQFSIPREKDACKVEVVVNEPITQRVGRLTPQVFDCHFLANEVQYTHNGCPILDEDEVMLRLYRFTETETFTETFLLRVKLIEPDCNIIQLGPKKLEVPEFYGLSDVIDKNILSFDYDKRINIECTIRISSSESFLPAHGQLVTGDPVKQEPRGDQPHSFFHGSNQKPGPLCKNGGCIQGLKRIQSVIKMSCEDFLVKGIRYQHLDPPSPNVDYISIRLDLTDSRSKSLYKSEHAWIPVLIRDAVPNQIPKANFMSMFILEVDQFVLTPISTATLDAEDSETAKHLLVFNITLPPSKGFITHLSDHTKPITSFTWKDLNEMLIAYQPPNSSHTERRNLEVEFEVHDFFFEKSPPITVHISIRTADTNAPRVSWNMGLDLLEGQSRPITWKQFQIVDNDNINAVRLVIVDGLQHGRITLRGGKGFIFTVKDIQAGDVCYHHDDSDTTKDFIVFRIFDGRHSIRHKFPINILPKDDSPPFLITNIVIELSEGQAVLIEGSMLCASDMDSSDAYIRFNITKPSVAGEIIKKPGQNLIAYPVTSFLQRDLYNGFIYYRHLGGEVFEDSFDVVLSDSHDPPNFSGPQAVIIHISPVDDQLPIEAPGTVRHLMVKENEVVYITKKQLHFIDPESPERELIYTVATPPCMVPTYSLDAGKIVLVDTILKLGKDYAGDTARTFSQHAVNHLKVAYIPPAKDIGPTPLHVQFILSVSNQHGGTVQGICFNITILPIDNQPPEVFSYDLKVEEGGFSQITASNIHITDKDTRQEQIKVILHKAPLHGIVQLDGSALQEGDPFTCLDLITLKVRYLHDGSETLQDEISFIATDGINSSEFILKIQVSPINDEPPFIKENLISTIICKEGAEVVISADYISATDADSDDMKLIFMIARQPRYGILLKAGSIIDQFTQRDVVEGLVHYRHTSGEIGLKVYVDIITLVVSDAEADKPQKKCCNNGPRLPILPNHESFPVYDLNVTILPVDNQPPSVIIGEIFVVDEGKSSALSVDHLSAMDPDTESNNLEFILVSPPRYGYIENVLPSPGFEKSNMGISIAAFLLKDIKSLNINYVQSRHQRIEPTTDQFTLYVTDGKLRSVEKPFYIVINPTNDEVPEFLARNITVVEGQMKKLDPTVINVSDMDVPQNHLYFTVIEQPRHGLIIEESYISGHMNRSQLIKRHQANSLPVIQDFSMDLLKNGMKMIYMHDDSENLADSFTIQLSDGKHEVRRTIFVNVIAVNDEKPVLTKHCDLVLSMGEQTLISSAILSAEDMDTPRTNISYVIDRCPEHGQLQMKVNKNWVTLHTGMKCTQEDIDMNLVRYVHTGTMGSKNEDTFTFHLWDGGNRSPEISVHIYVKDMEKGDIAVFVKMLNVSKGERVIITTNVLLATDGTDRPEELLYVLSSPPQYGQIEYVKHPGIPITSFSQMDVAAHTICYVHDFNSAATTDMFRFLVSNGLKTKHGIMNIALETVDRTLPTLSRNDGLRQVRGSMECISPATLQALDPDTSSQNITFQLVGLPQYGQLYLRGFPLQQDHFTQRDIDNLDIAYKHAGQDAQIDRFMFTVTDKANQGFIINGKIQTEPVPFTIQVDPVDSTAPRIVHLQCLTEVELLKNGQYGIYITSRHLKASDPDTDDERIVFHILGGPHYGYLENVTTGRFIRERFTQRDLNSKSVLYVINPSVDVFHDNIEFEVSDPVGNRALPQILELKWSQIELLQGMYEVCETEAVVSLKMTRKGYLLESAFVSLKVNEISAVLGKDISMNPSRLIQFDPGVSTKTWNIVIIQDGLEEDDEVFEVVLSSPVNAVLGSKTRAVVKIVDPRGGGCSSSKVSMIKTTGMGLLNCTVKPSRKPCAVFSFKETADKVREDVQEDVHTLQLPRKRLRLTGNRKTVKPSSVFRNKTDIIYQYHGIVSLRVEEDKPVLNETKKAKVLVKNRGQRKINAPSQNPDILQSGRTMLDSHVTTGGQDNAISFPKRCTPSLKGLLHFEDTVQKLFQCDGISWNPWITTKDSKAGKCPFGWTYHDGYCYILINDQKATWITAARACREQHFATLVDVFSKEQMEWLWNFSGRKSFWIGLNDRVRNGNWEWSKGEMVTFTNWKRGSPRSSHKGKNCVLAQKRGKWQAKDCKKGKGHYYICSKKL

Target ORF information:

RefSeq Version	XM_031890521.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis FRAS1 related extracellular matrix 1 (frem1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031890521.1

ORF Insert Sequence:

ATGAAGCCTT CTATTGTATA CCCAGTTTTG CTGTCAGTGT TGCTCACAAG GGGCCTCTGC 
GCCTCCTTTA TCAAAGTCAA TCACGGAATG AAAGTTATGA AAGGACAGAG TGCATTCCTC 
TCAGAAAGTG ACCTGCAGTT TTCCATCCCA AGGGAAAAGG ATGCCTGCAA AGTGGAAGTA 
GTTGTTAATG AACCCATTAC ACAGAGAGTT GGAAGGTTAA CACCACAGGT GTTTGACTGC 
CATTTCCTAG CCAATGAAGT TCAGTACACA CACAATGGCT GCCCTATCTT GGATGAAGAC 
GAGGTCATGC TCAGACTTTA CAGATTTACA GAAACTGAGA CTTTTACTGA AACCTTCCTG 
CTGAGAGTGA AGTTAATAGA GCCTGACTGT AATATCATAC AGCTGGGGCC TAAAAAGCTT 
GAAGTGCCAG AGTTTTATGG TCTGTCTGAC GTTATTGATA AGAACATACT GAGCTTTGAC 
TATGACAAAA GAATAAACAT TGAATGTACC ATCAGAATAT CGTCTTCTGA ATCTTTCCTT 
CCTGCACACG GTCAGCTTGT GACGGGGGAT CCTGTGAAAC AAGAACCACG AGGAGATCAG 
CCACACAGCT TCTTCCATGG ATCAAATCAA AAGCCAGGAC CTTTGTGCAA GAATGGAGGC 
TGCATACAAG GTTTAAAGAG GATCCAGAGC GTTATAAAAA TGAGTTGTGA AGATTTCCTT 
GTAAAGGGCA TCCGATACCA GCACTTGGAT CCTCCATCTC CAAATGTTGA TTATATCAGT 
ATCAGGTTGG ATCTTACAGA CTCAAGGAGC AAATCACTTT ACAAGTCAGA ACATGCTTGG 
ATTCCTGTTC TCATTCGGGA TGCTGTTCCA AATCAAATCC CCAAAGCCAA CTTCATGTCT 
ATGTTTATCT TAGAAGTAGA TCAGTTTGTT CTAACACCCA TCTCAACTGC AACACTGGAT 
GCCGAGGACA GCGAAACAGC CAAACATCTT CTGGTCTTCA ATATTACTTT ACCTCCAAGC 
AAAGGGTTCA TCACACACTT GTCTGACCAT ACCAAGCCCA TTACATCCTT TACATGGAAA 
GATCTCAATG AAATGCTCAT AGCATATCAG CCTCCGAACA GCAGCCACAC AGAGAGAAGA 
AATTTGGAGG TGGAGTTTGA GGTGCATGAT TTCTTTTTTG AAAAGAGTCC TCCAATTACA 
GTGCATATCT CTATACGTAC GGCTGACACC AATGCTCCTC GCGTTTCATG GAACATGGGT 
CTGGATTTAC TGGAGGGCCA GTCTCGACCA ATTACATGGA AACAGTTCCA AATTGTGGAT 
AATGACAACA TCAATGCAGT TCGTTTGGTC ATTGTAGATG GATTGCAGCA TGGACGTATT 
ACTCTGAGAG GTGGGAAAGG ATTTATATTC ACAGTTAAGG ACATCCAGGC TGGAGATGTT 
TGCTACCATC ATGATGACAG TGACACTACT AAGGATTTTA TAGTCTTCAG AATATTTGAT 
GGACGTCACA GCATACGACA CAAGTTTCCT ATTAACATCC TACCAAAAGA TGATAGCCCT 
CCCTTCCTCA TCACAAACAT TGTTATTGAG CTTAGTGAAG GTCAAGCTGT CCTTATCGAG 
GGATCTATGC TTTGTGCTTC TGATATGGAC TCTAGTGATG CATACATACG TTTTAATATC 
ACTAAGCCTT CTGTAGCTGG AGAGATTATA AAGAAGCCAG GACAAAATCT TATTGCTTAT 
CCTGTCACAA GCTTCCTGCA ACGAGACTTA TATAACGGTT TCATTTATTA TCGCCATCTT 
GGAGGAGAAG TATTTGAAGA TTCATTTGAT GTTGTTCTGT CTGATAGCCA TGATCCACCA 
AACTTTTCTG GTCCTCAGGC AGTAATCATT CATATTAGCC CCGTGGATGA CCAGCTGCCC 
ATAGAAGCAC CTGGAACAGT ACGACATCTG ATGGTAAAGG AGAATGAGGT TGTTTATATA 
ACAAAGAAAC AACTGCATTT CATAGACCCA GAGTCACCTG AAAGAGAACT TATTTACACA 
GTTGCTACTC CTCCTTGTAT GGTGCCAACC TACAGCTTAG ATGCGGGGAA GATAGTTCTT 
GTAGACACTA TACTTAAACT TGGAAAGGAT TATGCAGGAG ACACAGCAAG AACATTTTCA 
CAGCATGCTG TGAATCACCT CAAAGTTGCT TACATACCAC CAGCTAAAGA CATTGGGCCA 
ACTCCTCTAC ATGTCCAGTT TATATTATCA GTGAGTAACC AACATGGAGG AACAGTTCAA 
GGCATCTGTT TCAATATCAC TATCCTTCCA ATAGACAATC AGCCTCCTGA GGTGTTTTCA 
TATGATTTGA AAGTTGAAGA AGGTGGTTTC AGCCAAATTA CTGCAAGTAA CATTCACATC 
ACAGATAAGG ACACCAGACA AGAACAAATT AAAGTTATTT TACACAAAGC TCCACTGCAT 
GGTATCGTTC AACTTGATGG ATCTGCATTG CAGGAAGGTG ACCCATTCAC CTGCTTGGAC 
CTCATCACAT TAAAAGTTAG GTACCTTCAT GATGGTTCAG AGACCCTACA AGATGAAATC 
TCATTTATTG CAACAGATGG AATCAATTCA TCTGAGTTTA TTTTAAAAAT ACAGGTTTCT 
CCTATTAATG ATGAACCTCC ATTTATTAAA GAGAACCTAA TTTCCACCAT TATTTGCAAA 
GAAGGGGCAG AGGTAGTCAT ATCAGCAGAC TATATCTCAG CTACTGATGC AGACAGTGAT 
GACATGAAGC TGATATTTAT GATCGCTCGC CAACCACGTT ATGGCATTTT GCTAAAGGCT 
GGATCTATCA TTGATCAATT CACCCAGAGA GATGTTGTTG AAGGACTGGT ACATTATAGA 
CATACAAGTG GCGAAATTGG CTTGAAGGTC TATGTTGATA TTATAACACT GGTGGTTTCT 
GATGCTGAAG CAGATAAACC GCAGAAAAAG TGCTGCAACA ATGGTCCTCG TCTGCCGATT 
TTGCCAAACC ATGAGTCTTT TCCTGTATAT GATCTCAATG TTACAATTCT GCCAGTGGAC 
AACCAACCCC CATCGGTTAT AATAGGTGAA ATATTTGTAG TCGATGAGGG CAAATCATCT 
GCCCTCAGTG TAGATCATTT AAGTGCCATG GACCCTGACA CAGAAAGTAA CAATCTGGAA 
TTCATACTGG TTTCTCCTCC TCGATATGGT TACATTGAAA ATGTCCTCCC ATCTCCTGGA 
TTTGAGAAGA GTAATATGGG TATAAGTATT GCTGCATTTC TACTCAAGGA CATTAAATCT 
CTGAACATCA ATTATGTCCA GTCCCGCCAT CAAAGAATTG AACCAACCAC TGATCAGTTT 
ACACTGTATG TCACTGATGG CAAACTGCGT TCTGTAGAAA AGCCATTTTA CATCGTAATC 
AATCCGACAA ATGATGAGGT TCCGGAATTT TTAGCCAGAA ACATAACAGT TGTTGAAGGA 
CAAATGAAGA AATTGGATCC CACTGTAATA AATGTGTCAG ACATGGATGT GCCACAGAAC 
CACTTATACT TTACTGTTAT AGAGCAGCCC CGACATGGAC TGATTATTGA GGAATCGTAT 
ATCAGTGGGC ACATGAATAG AAGCCAATTA ATAAAAAGGC ATCAGGCAAA CAGCCTGCCT 
GTGATACAAG ATTTTTCCAT GGACCTGCTA AAAAATGGAA TGAAGATGAT ATACATGCAT 
GATGACTCAG AAAACCTTGC TGACAGCTTC ACTATCCAGC TATCTGATGG AAAGCATGAA 
GTCAGGAGAA CTATTTTTGT GAATGTAATT GCAGTTAATG ACGAAAAGCC TGTGCTAACC 
AAGCACTGCG ATTTGGTGCT AAGCATGGGG GAACAAACTC TTATATCAAG TGCCATACTG 
TCAGCAGAAG ATATGGACAC ACCCAGGACA AACATTTCCT ATGTTATTGA CAGATGTCCA 
GAGCATGGCC AGCTTCAGAT GAAAGTCAAT AAAAACTGGG TGACCTTGCA TACTGGAATG 
AAATGCACTC AGGAGGACAT TGATATGAAC CTGGTTAGAT ATGTGCATAC TGGAACAATG 
GGCTCAAAAA ATGAAGACAC GTTTACTTTC CACCTTTGGG ATGGCGGAAA CCGATCACCT 
GAGATATCTG TTCATATTTA TGTCAAGGAT ATGGAAAAAG GAGATATTGC AGTTTTTGTG 
AAAATGCTTA ATGTGTCCAA GGGAGAGAGA GTTATCATAA CAACAAATGT CCTTCTCGCC 
ACTGATGGGA CCGACAGACC AGAAGAGCTG CTGTATGTGT TATCCTCGCC ACCCCAATAT 
GGGCAGATTG AATATGTTAA GCATCCTGGA ATCCCCATAA CAAGTTTCAG TCAGATGGAT 
GTGGCTGCTC ACACAATCTG CTACGTCCAC GATTTCAATT CAGCAGCCAC TACTGATATG 
TTTAGATTCC TAGTCAGCAA TGGGCTGAAA ACGAAACATG GGATCATGAA CATCGCACTG 
GAAACAGTCG ATCGAACTCT TCCAACACTA TCCAGGAACG ATGGTCTAAG ACAAGTGAGA 
GGGTCTATGG AGTGTATTTC CCCGGCCACC CTACAGGCAC TGGACCCTGA CACGTCATCT 
CAGAACATTA CTTTTCAGTT GGTGGGTCTC CCTCAATATG GGCAATTATA CCTCAGAGGC 
TTTCCACTAC AGCAAGATCA CTTCACCCAA AGAGATATTG ACAATCTAGA CATTGCCTAT 
AAGCATGCTG GACAGGATGC TCAAATAGAC AGGTTTATGT TTACTGTTAC AGACAAAGCA 
AATCAAGGAT TTATCATTAA TGGGAAGATT CAGACGGAAC CAGTTCCATT CACAATACAG 
GTAGATCCTG TGGACAGCAC AGCTCCCAGA ATTGTCCATC TTCAATGCCT TACAGAAGTT 
GAGCTTCTGA AAAATGGCCA GTATGGAATT TATATTACTT CAAGACACTT AAAGGCATCT 
GATCCAGACA CAGATGATGA AAGAATTGTC TTTCACATAT TAGGAGGGCC TCACTATGGT 
TATTTAGAGA ATGTTACAAC AGGCAGGTTT ATAAGAGAAA GATTTACACA AAGGGATTTA 
AACAGCAAAT CAGTGCTCTA CGTAATAAAT CCATCAGTGG ATGTTTTTCA TGACAACATA 
GAGTTTGAAG TTTCAGACCC CGTTGGAAAC AGAGCTTTAC CACAAATTTT AGAGCTGAAA 
TGGAGTCAAA TTGAATTGCT CCAAGGCATG TATGAGGTGT GTGAAACCGA GGCAGTTGTG 
TCTTTGAAGA TGACAAGAAA AGGTTATCTC CTGGAGTCTG CTTTTGTTTC GCTCAAGGTG 
AATGAAATAA GTGCTGTTCT TGGCAAAGAC ATTTCCATGA ATCCTTCCAG ACTTATACAG 
TTTGATCCAG GAGTGTCAAC CAAGACTTGG AATATAGTAA TTATCCAGGA TGGATTAGAA 
GAAGATGACG AGGTCTTTGA AGTAGTTCTG AGTTCTCCTG TGAATGCTGT TCTTGGCTCT 
AAGACAAGAG CTGTAGTGAA AATTGTGGAC CCCAGAGGAG GTGGCTGCAG TTCCTCCAAA 
GTCAGCATGA TCAAAACAAC TGGAATGGGC TTACTAAACT GTACCGTCAA ACCCTCACGC 
AAACCTTGTG CTGTTTTTAG TTTTAAAGAG ACTGCAGATA AGGTCAGGGA AGATGTCCAA 
GAAGATGTCC ATACCTTACA ACTTCCGAGG AAGAGACTAA GACTCACAGG GAACAGAAAA 
ACAGTTAAAC CATCATCAGT ATTTAGAAAC AAAACAGACA TTATTTATCA ATATCATGGG 
ATTGTGTCTC TCCGAGTGGA AGAAGATAAG CCAGTATTAA ATGAAACAAA GAAAGCAAAA 
GTACTAGTGA AGAACCGTGG TCAGAGGAAG ATAAATGCCC CCTCCCAAAA TCCTGACATT 
CTTCAATCAG GCAGAACCAT GTTGGATTCT CATGTTACTA CTGGTGGTCA GGATAACGCA 
ATATCGTTTC CTAAAAGATG CACACCAAGT TTAAAAGGAC TCCTGCATTT TGAGGACACA 
GTACAGAAAC TGTTCCAGTG TGACGGCATA TCGTGGAACC CCTGGATCAC TACTAAGGAT 
TCTAAAGCAG GAAAATGCCC ATTTGGCTGG ACTTACCATG ATGGCTACTG CTATATACTG 
ATCAATGATC AAAAGGCCAC CTGGATAACA GCAGCCAGAG CATGTAGAGA ACAGCATTTT 
GCTACTCTGG TGGATGTATT CAGCAAGGAA CAAATGGAAT GGCTTTGGAA TTTCAGTGGC 
AGAAAATCCT TTTGGATAGG CCTCAATGAT AGAGTACGTA ATGGAAACTG GGAGTGGAGC 
AAAGGGGAAA TGGTCACCTT CACCAATTGG AAAAGAGGCT CTCCTCGATC TTCACACAAA 
GGGAAGAATT GCGTTTTGGC ACAAAAGAGA GGCAAATGGC AGGCTAAAGA CTGCAAAAAA 
GGCAAAGGCC ATTACTATAT ATGTTCAAAA AAGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

frem1 ( XM_002939090.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002939136.2 Xenopus tropicalis FRAS1 related extracellular matrix 1 (frem1), transcript variant X1, mRNA.

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CloneID	OXa04017
Clone ID Related Accession (Same CDS sequence)	XM_002939090.4 , XM_002939090.5
Accession Version	XM_002939090.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	FRAS1-related extracellular matrix protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002939090.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCCTT CTATTGTATA CCCAGTTTTG CTGTCAGTGT TGCTCACAAG GGGCCTCTGC 
GCCTCCTTTA TCAAAGTCAA TCACGGAATG AAAGTTATGA AAGGACAGAG TGCATTCCTC 
TCAGAAAGTG ACCTGCAGTT TTCCATCCCA AGGGAAAAGG ATGCCTGCAA AGTGGAAGTA 
GTTGTTAATG AACCCATTAC ACAGAGAGTT GGAAGGTTAA CACCACAGGT GTTTGACTGC 
CATTTCCTAG CCAATGAAGT TCAGTACACA CACAATGGCT GCCCTATCTT GGATGAAGAC 
GAGGTCATGC TCAGACTTTA CAGATTTACA GAAACTGAGA CTTTTACTGA AACCTTCCTG 
CTGAGAGTGA AGTTAATAGA GCCTGACTGT AATATCATAC AGCTGGGGCC TAAAAAGCTT 
GAAGTGCCAG AGTTTTATGG TCTGTCTGAC GTTATTGATA AGAACATACT GAGCTTTGAC 
TATGACAAAA GAATAAACAT TGAATGTACC ATCAGAATAT CGTCTTCTGA ATCTTTCCTT 
CCTGCACACG GTCAGCTTGT GACGGGGGAT CCTGTGAAAC AAGAACCACG AGGAGATCAG 
CCACACAGCT TCTTCCATGG ATCAAATCAA AAGCCAGGAC CTTTGTGCAA GAATGGAGGC 
TGCATACAAG GTTTAAAGAG GATCCAGAGC GTTATAAAAA TGAGTTGTGA AGATTTCCTT 
GTAAAGGGCA TCCGATACCA GCACTTGGAT CCTCCATCTC CAAATGTTGA TTATATCAGT 
ATCAGGTTGG ATCTTACAGA CTCAAGGAGC AAATCACTTT ACAAGTCAGA ACATGCTTGG 
ATTCCTGTTC TCATTCGGGA TGCTGTTCCA AATCAAATCC CCAAAGCCAA CTTCATGTCT 
ATGTTTATCT TAGAAGTAGA TCAGTTTGTT CTAACACCCA TCTCAACTGC AACACTGGAT 
GCCGAGGACA GCGAAACAGC CAAACATCTT CTGGTCTTCA ATATTACTTT ACCTCCAAGC 
AAAGGGTTCA TCACACACTT GTCTGACCAT ACCAAGCCCA TTACATCCTT TACATGGAAA 
GATCTCAATG AAATGCTCAT AGCATATCAG CCTCCGAACA GCAGCCACAC AGAGAGAAGA 
AATTTGGAGG TGGAGTTTGA GGTGCATGAT TTCTTTTTTG AAAAGAGTCC TCCAATTACA 
GTGCATATCT CTATACGTAC GGCTGACACC AATGCTCCTC GCGTTTCATG GAACATGGGT 
CTGGATTTAC TGGAGGGCCA GTCTCGACCA ATTACATGGA AACAGTTCCA AATTGTGGAT 
AATGACAACA TCAATGCAGT TCGTTTGGTC ATTGTAGATG GATTGCAGCA TGGACGTATT 
ACTCTGAGAG GTGGGAAAGG ATTTATATTC ACAGTTAAGG ACATCCAGGC TGGAGATGTT 
TGCTACCATC ATGATGACAG TGACACTACT AAGGATTTTA TAGTCTTCAG AATATTTGAT 
GGACGTCACA GCATACGACA CAAGTTTCCT ATTAACATCC TACCAAAAGA TGATAGCCCT 
CCCTTCCTCA TCACAAACAT TGTTATTGAG CTTAGTGAAG GTCAAGCTGT CCTTATCGAG 
GGATCTATGC TTTGTGCTTC TGATATGGAC TCTAGTGATG CATACATACG TTTTAATATC 
ACTAAGCCTT CTGTAGCTGG AGAGATTATA AAGAAGCCAG GACAAAATCT TATTGCTTAT 
CCTGTCACAA GCTTCCTGCA ACGAGACTTA TATAACGGTT TCATTTATTA TCGCCATCTT 
GGAGGAGAAG TATTTGAAGA TTCATTTGAT GTTGTTCTGT CTGATAGCCA TGATCCACCA 
AACTTTTCTG GTCCTCAGGC AGTAATCATT CATATTAGCC CCGTGGATGA CCAGCTGCCC 
ATAGAAGCAC CTGGAACAGT ACGACATCTG ATGGTAAAGG AGAATGAGGT TGTTTATATA 
ACAAAGAAAC AACTGCATTT CATAGACCCA GAGTCACCTG AAAGAGAACT TATTTACACA 
GTTGCTACTC CTCCTTGTAT GGTGCCAACC TACAGCAGCT TAGATGCGGG GAAGATAGTT 
CTTGTAGACA CTATACTTAA ACTTGGAAAG GATTATGCAG GAGACACAGC AAGAACATTT 
TCACAGCATG CTGTGAATCA CCTCAAAGTT GCTTACATAC CACCAGCTAA AGACATTGGG 
CCAACTCCTC TACATGTCCA GTTTATATTA TCAGTGAGTA ACCAACATGG AGGAACAGTT 
CAAGGCATCT GTTTCAATAT CACTATCCTT CCAATAGACA ATCAGCCTCC TGAGGTGTTT 
TCATATGATT TGAAAGTTGA AGAAGGTGGT TTCAGCCAAA TTACTGCAAG TAACATTCAC 
ATCACAGATA AGGACACCAG ACAAGAACAA ATTAAAGTTA TTTTACACAA AGCTCCACTG 
CATGGTATCG TTCAACTTGA TGGATCTGCA TTGCAGGAAG GTGACCCATT CACCTGCTTG 
GACCTCATCA CATTAAAAGT TAGGTACCTT CATGATGGTT CAGAGACCCT ACAAGATGAA 
ATCTCATTTA TTGCAACAGA TGGAATCAAT TCATCTGAGT TTATTTTAAA AATACAGGTT 
TCTCCTATTA ATGATGAACC TCCATTTATT AAAGAGAACC TAATTTCCAC CATTATTTGC 
AAAGAAGGGG CAGAGGTAGT CATATCAGCA GACTATATCT CAGCTACTGA TGCAGACAGT 
GATGACATGA AGCTGATATT TATGATCGCT CGCCAACCAC GTTATGGCAT TTTGCTAAAG 
GCTGGATCTA TCATTGATCA ATTCACCCAG AGAGATGTTG TTGAAGGACT GGTACATTAT 
AGACATACAA GTGGCGAAAT TGGCTTGAAG GTCTATGTTG ATATTATAAC ACTGGTGGTT 
TCTGATGCTG AAGCAGATAA ACCGCAGAAA AAGTGCTGCA ACAATGGTCC TCGTCTGCCG 
ATTTTGCCAA ACCATGAGTC TTTTCCTGTA TATGATCTCA ATGTTACAAT TCTGCCAGTG 
GACAACCAAC CCCCATCGGT TATAATAGGT GAAATATTTG TAGTCGATGA GGGCAAATCA 
TCTGCCCTCA GTGTAGATCA TTTAAGTGCC ATGGACCCTG ACACAGAAAG TAACAATCTG 
GAATTCATAC TGGTTTCTCC TCCTCGATAT GGTTACATTG AAAATGTCCT CCCATCTCCT 
GGATTTGAGA AGAGTAATAT GGGTATAAGT ATTGCTGCAT TTCTACTCAA GGACATTAAA 
TCTCTGAACA TCAATTATGT CCAGTCCCGC CATCAAAGAA TTGAACCAAC CACTGATCAG 
TTTACACTGT ATGTCACTGA TGGCAAACTG CGTTCTGTAG AAAAGCCATT TTACATCGTA 
ATCAATCCGA CAAATGATGA GGTTCCGGAA TTTTTAGCCA GAAACATAAC AGTTGTTGAA 
GGACAAATGA AGAAATTGGA TCCCACTGTA ATAAATGTGT CAGACATGGA TGTGCCACAG 
AACCACTTAT ACTTTACTGT TATAGAGCAG CCCCGACATG GACTGATTAT TGAGGAATCG 
TATATCAGTG GGCACATGAA TAGAAGCCAA TTAATAAAAA GGCATCAGGC AAACAGCCTG 
CCTGTGATAC AAGATTTTTC CATGGACCTG CTAAAAAATG GAATGAAGAT GATATACATG 
CATGATGACT CAGAAAACCT TGCTGACAGC TTCACTATCC AGCTATCTGA TGGAAAGCAT 
GAAGTCAGGA GAACTATTTT TGTGAATGTA ATTGCAGTTA ATGACGAAAA GCCTGTGCTA 
ACCAAGCACT GCGATTTGGT GCTAAGCATG GGGGAACAAA CTCTTATATC AAGTGCCATA 
CTGTCAGCAG AAGATATGGA CACACCCAGG ACAAACATTT CCTATGTTAT TGACAGATGT 
CCAGAGCATG GCCAGCTTCA GATGAAAGTC AATAAAAACT GGGTGACCTT GCATACTGGA 
ATGAAATGCA CTCAGGAGGA CATTGATATG AACCTGGTTA GATATGTGCA TACTGGAACA 
ATGGGCTCAA AAAATGAAGA CACGTTTACT TTCCACCTTT GGGATGGCGG AAACCGATCA 
CCTGAGATAT CTGTTCATAT TTATGTCAAG GATATGGAAA AAGGAGATAT TGCAGTTTTT 
GTGAAAATGC TTAATGTGTC CAAGGGAGAG AGAGTTATCA TAACAACAAA TGTCCTTCTC 
GCCACTGATG GGACCGACAG ACCAGAAGAG CTGCTGTATG TGTTATCCTC GCCACCCCAA 
TATGGGCAGA TTGAATATGT TAAGCATCCT GGAATCCCCA TAACAAGTTT CAGTCAGATG 
GATGTGGCTG CTCACACAAT CTGCTACGTC CACGATTTCA ATTCAGCAGC CACTACTGAT 
ATGTTTAGAT TCCTAGTCAG CAATGGGCTG AAAACGAAAC ATGGGATCAT GAACATCGCA 
CTGGAAACAG TCGATCGAAC TCTTCCAACA CTATCCAGGA ACGATGGTCT AAGACAAGTG 
AGAGGGTCTA TGGAGTGTAT TTCCCCGGCC ACCCTACAGG CACTGGACCC TGACACGTCA 
TCTCAGAACA TTACTTTTCA GTTGGTGGGT CTCCCTCAAT ATGGGCAATT ATACCTCAGA 
GGCTTTCCAC TACAGCAAGA TCACTTCACC CAAAGAGATA TTGACAATCT AGACATTGCC 
TATAAGCATG CTGGACAGGA TGCTCAAATA GACAGGTTTA TGTTTACTGT TACAGACAAA 
GCAAATCAAG GATTTATCAT TAATGGGAAG ATTCAGACGG AACCAGTTCC ATTCACAATA 
CAGGTAGATC CTGTGGACAG CACAGCTCCC AGAATTGTCC ATCTTCAATG CCTTACAGAA 
GTTGAGCTTC TGAAAAATGG CCAGTATGGA ATTTATATTA CTTCAAGACA CTTAAAGGCA 
TCTGATCCAG ACACAGATGA TGAAAGAATT GTCTTTCACA TATTAGGAGG GCCTCACTAT 
GGTTATTTAG AGAATGTTAC AACAGGCAGG TTTATAAGAG AAAGATTTAC ACAAAGGGAT 
TTAAACAGCA AATCAGTGCT CTACGTAATA AATCCATCAG TGGATGTTTT TCATGACAAC 
ATAGAGTTTG AAGTTTCAGA CCCCGTTGGA AACAGAGCTT TACCACAAAT TTTAGAGCTG 
AAATGGAGTC AAATTGAATT GCTCCAAGGC ATGTATGAGG TGTGTGAAAC CGAGGCAGTT 
GTGTCTTTGA AGATGACAAG AAAAGGTTAT CTCCTGGAGT CTGCTTTTGT TTCGCTCAAG 
GTGAATGAAA TAAGTGCTGT TCTTGGCAAA GACATTTCCA TGAATCCTTC CAGACTTATA 
CAGTTTGATC CAGGAGTGTC AACCAAGACT TGGAATATAG TAATTATCCA GGATGGATTA 
GAAGAAGATG ACGAGGTCTT TGAAGTAGTT CTGAGTTCTC CTGTGAATGC TGTTCTTGGC 
TCTAAGACAA GAGCTGTAGT GAAAATTGTG GACCCCAGAG GAGGTGGCTG CAGTTCCTCC 
AAAGTCAGCA TGATCAAAAC AACTGGAATG GGCTTACTAA ACTGTACCGT CAAACCCTCA 
CGCAAACCTT GTGCTGTTTT TAGTTTTAAA GAGACTGCAG ATAAGGTCAG GGAAGATGTC 
CAAGAAGATG TCCATACCTT ACAACTTCCG AGGAAGAGAC TAAGACTCAC AGGGAACAGA 
AAAACAGTTA AACCATCATC AGTATTTAGA AACAAAACAG ACATTATTTA TCAATATCAT 
GGGATTGTGT CTCTCCGAGT GGAAGAAGAT AAGCCAGTAT TAAATGAAAC AAAGAAAGCA 
AAAGTACTAG TGAAGAACCG TGGTCAGAGG AAGATAAATG CCCCCTCCCA AAATCCTGAC 
ATTCTTCAAT CAGGCAGAAC CATGTTGGAT TCTCATGTTA CTACTGGTGG TCAGGATAAC 
GCAATATCGT TTCCTAAAAG ATGCACACCA AGTTTAAAAG GACTCCTGCA TTTTGAGGAC 
ACAGTACAGA AACTGTTCCA GTGTGACGGC ATATCGTGGA ACCCCTGGAT CACTACTAAG 
GATTCTAAAG CAGGAAAATG CCCATTTGGC TGGACTTACC ATGATGGCTA CTGCTATATA 
CTGATCAATG ATCAAAAGGC CACCTGGATA ACAGCAGCCA GAGCATGTAG AGAACAGCAT 
TTTGCTACTC TGGTGGATGT ATTCAGCAAG GAACAAATGG AATGGCTTTG GAATTTCAGT 
GGCAGAAAAT CCTTTTGGAT AGGCCTCAAT GATAGAGTAC GTAATGGAAA CTGGGAGTGG 
AGCAAAGGGG AAATGGTCAC CTTCACCAAT TGGAAAAGAG GCTCTCCTCG ATCTTCACAC 
AAAGGGAAGA ATTGCGTTTT GGCACAAAAG AGAGGCAAAT GGCAGGCTAA AGACTGCAAA 
AAAGGCAAAG GCCATTACTA TATATGTTCA AAAAAGTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002939136.2
CDS	608..7129
Translation	MKPSIVYPVLLSVLLTRGLCASFIKVNHGMKVMKGQSAFLSESDLQFSIPREKDACKVEVVVNEPITQRVGRLTPQVFDCHFLANEVQYTHNGCPILDEDEVMLRLYRFTETETFTETFLLRVKLIEPDCNIIQLGPKKLEVPEFYGLSDVIDKNILSFDYDKRINIECTIRISSSESFLPAHGQLVTGDPVKQEPRGDQPHSFFHGSNQKPGPLCKNGGCIQGLKRIQSVIKMSCEDFLVKGIRYQHLDPPSPNVDYISIRLDLTDSRSKSLYKSEHAWIPVLIRDAVPNQIPKANFMSMFILEVDQFVLTPISTATLDAEDSETAKHLLVFNITLPPSKGFITHLSDHTKPITSFTWKDLNEMLIAYQPPNSSHTERRNLEVEFEVHDFFFEKSPPITVHISIRTADTNAPRVSWNMGLDLLEGQSRPITWKQFQIVDNDNINAVRLVIVDGLQHGRITLRGGKGFIFTVKDIQAGDVCYHHDDSDTTKDFIVFRIFDGRHSIRHKFPINILPKDDSPPFLITNIVIELSEGQAVLIEGSMLCASDMDSSDAYIRFNITKPSVAGEIIKKPGQNLIAYPVTSFLQRDLYNGFIYYRHLGGEVFEDSFDVVLSDSHDPPNFSGPQAVIIHISPVDDQLPIEAPGTVRHLMVKENEVVYITKKQLHFIDPESPERELIYTVATPPCMVPTYSSLDAGKIVLVDTILKLGKDYAGDTARTFSQHAVNHLKVAYIPPAKDIGPTPLHVQFILSVSNQHGGTVQGICFNITILPIDNQPPEVFSYDLKVEEGGFSQITASNIHITDKDTRQEQIKVILHKAPLHGIVQLDGSALQEGDPFTCLDLITLKVRYLHDGSETLQDEISFIATDGINSSEFILKIQVSPINDEPPFIKENLISTIICKEGAEVVISADYISATDADSDDMKLIFMIARQPRYGILLKAGSIIDQFTQRDVVEGLVHYRHTSGEIGLKVYVDIITLVVSDAEADKPQKKCCNNGPRLPILPNHESFPVYDLNVTILPVDNQPPSVIIGEIFVVDEGKSSALSVDHLSAMDPDTESNNLEFILVSPPRYGYIENVLPSPGFEKSNMGISIAAFLLKDIKSLNINYVQSRHQRIEPTTDQFTLYVTDGKLRSVEKPFYIVINPTNDEVPEFLARNITVVEGQMKKLDPTVINVSDMDVPQNHLYFTVIEQPRHGLIIEESYISGHMNRSQLIKRHQANSLPVIQDFSMDLLKNGMKMIYMHDDSENLADSFTIQLSDGKHEVRRTIFVNVIAVNDEKPVLTKHCDLVLSMGEQTLISSAILSAEDMDTPRTNISYVIDRCPEHGQLQMKVNKNWVTLHTGMKCTQEDIDMNLVRYVHTGTMGSKNEDTFTFHLWDGGNRSPEISVHIYVKDMEKGDIAVFVKMLNVSKGERVIITTNVLLATDGTDRPEELLYVLSSPPQYGQIEYVKHPGIPITSFSQMDVAAHTICYVHDFNSAATTDMFRFLVSNGLKTKHGIMNIALETVDRTLPTLSRNDGLRQVRGSMECISPATLQALDPDTSSQNITFQLVGLPQYGQLYLRGFPLQQDHFTQRDIDNLDIAYKHAGQDAQIDRFMFTVTDKANQGFIINGKIQTEPVPFTIQVDPVDSTAPRIVHLQCLTEVELLKNGQYGIYITSRHLKASDPDTDDERIVFHILGGPHYGYLENVTTGRFIRERFTQRDLNSKSVLYVINPSVDVFHDNIEFEVSDPVGNRALPQILELKWSQIELLQGMYEVCETEAVVSLKMTRKGYLLESAFVSLKVNEISAVLGKDISMNPSRLIQFDPGVSTKTWNIVIIQDGLEEDDEVFEVVLSSPVNAVLGSKTRAVVKIVDPRGGGCSSSKVSMIKTTGMGLLNCTVKPSRKPCAVFSFKETADKVREDVQEDVHTLQLPRKRLRLTGNRKTVKPSSVFRNKTDIIYQYHGIVSLRVEEDKPVLNETKKAKVLVKNRGQRKINAPSQNPDILQSGRTMLDSHVTTGGQDNAISFPKRCTPSLKGLLHFEDTVQKLFQCDGISWNPWITTKDSKAGKCPFGWTYHDGYCYILINDQKATWITAARACREQHFATLVDVFSKEQMEWLWNFSGRKSFWIGLNDRVRNGNWEWSKGEMVTFTNWKRGSPRSSHKGKNCVLAQKRGKWQAKDCKKGKGHYYICSKKL

Target ORF information:

RefSeq Version	XM_002939090.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis FRAS1 related extracellular matrix 1 (frem1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002939090.4

ORF Insert Sequence:

ATGAAGCCTT CTATTGTATA CCCAGTTTTG CTGTCAGTGT TGCTCACAAG GGGCCTCTGC 
GCCTCCTTTA TCAAAGTCAA TCACGGAATG AAAGTTATGA AAGGACAGAG TGCATTCCTC 
TCAGAAAGTG ACCTGCAGTT TTCCATCCCA AGGGAAAAGG ATGCCTGCAA AGTGGAAGTA 
GTTGTTAATG AACCCATTAC ACAGAGAGTT GGAAGGTTAA CACCACAGGT GTTTGACTGC 
CATTTCCTAG CCAATGAAGT TCAGTACACA CACAATGGCT GCCCTATCTT GGATGAAGAC 
GAGGTCATGC TCAGACTTTA CAGATTTACA GAAACTGAGA CTTTTACTGA AACCTTCCTG 
CTGAGAGTGA AGTTAATAGA GCCTGACTGT AATATCATAC AGCTGGGGCC TAAAAAGCTT 
GAAGTGCCAG AGTTTTATGG TCTGTCTGAC GTTATTGATA AGAACATACT GAGCTTTGAC 
TATGACAAAA GAATAAACAT TGAATGTACC ATCAGAATAT CGTCTTCTGA ATCTTTCCTT 
CCTGCACACG GTCAGCTTGT GACGGGGGAT CCTGTGAAAC AAGAACCACG AGGAGATCAG 
CCACACAGCT TCTTCCATGG ATCAAATCAA AAGCCAGGAC CTTTGTGCAA GAATGGAGGC 
TGCATACAAG GTTTAAAGAG GATCCAGAGC GTTATAAAAA TGAGTTGTGA AGATTTCCTT 
GTAAAGGGCA TCCGATACCA GCACTTGGAT CCTCCATCTC CAAATGTTGA TTATATCAGT 
ATCAGGTTGG ATCTTACAGA CTCAAGGAGC AAATCACTTT ACAAGTCAGA ACATGCTTGG 
ATTCCTGTTC TCATTCGGGA TGCTGTTCCA AATCAAATCC CCAAAGCCAA CTTCATGTCT 
ATGTTTATCT TAGAAGTAGA TCAGTTTGTT CTAACACCCA TCTCAACTGC AACACTGGAT 
GCCGAGGACA GCGAAACAGC CAAACATCTT CTGGTCTTCA ATATTACTTT ACCTCCAAGC 
AAAGGGTTCA TCACACACTT GTCTGACCAT ACCAAGCCCA TTACATCCTT TACATGGAAA 
GATCTCAATG AAATGCTCAT AGCATATCAG CCTCCGAACA GCAGCCACAC AGAGAGAAGA 
AATTTGGAGG TGGAGTTTGA GGTGCATGAT TTCTTTTTTG AAAAGAGTCC TCCAATTACA 
GTGCATATCT CTATACGTAC GGCTGACACC AATGCTCCTC GCGTTTCATG GAACATGGGT 
CTGGATTTAC TGGAGGGCCA GTCTCGACCA ATTACATGGA AACAGTTCCA AATTGTGGAT 
AATGACAACA TCAATGCAGT TCGTTTGGTC ATTGTAGATG GATTGCAGCA TGGACGTATT 
ACTCTGAGAG GTGGGAAAGG ATTTATATTC ACAGTTAAGG ACATCCAGGC TGGAGATGTT 
TGCTACCATC ATGATGACAG TGACACTACT AAGGATTTTA TAGTCTTCAG AATATTTGAT 
GGACGTCACA GCATACGACA CAAGTTTCCT ATTAACATCC TACCAAAAGA TGATAGCCCT 
CCCTTCCTCA TCACAAACAT TGTTATTGAG CTTAGTGAAG GTCAAGCTGT CCTTATCGAG 
GGATCTATGC TTTGTGCTTC TGATATGGAC TCTAGTGATG CATACATACG TTTTAATATC 
ACTAAGCCTT CTGTAGCTGG AGAGATTATA AAGAAGCCAG GACAAAATCT TATTGCTTAT 
CCTGTCACAA GCTTCCTGCA ACGAGACTTA TATAACGGTT TCATTTATTA TCGCCATCTT 
GGAGGAGAAG TATTTGAAGA TTCATTTGAT GTTGTTCTGT CTGATAGCCA TGATCCACCA 
AACTTTTCTG GTCCTCAGGC AGTAATCATT CATATTAGCC CCGTGGATGA CCAGCTGCCC 
ATAGAAGCAC CTGGAACAGT ACGACATCTG ATGGTAAAGG AGAATGAGGT TGTTTATATA 
ACAAAGAAAC AACTGCATTT CATAGACCCA GAGTCACCTG AAAGAGAACT TATTTACACA 
GTTGCTACTC CTCCTTGTAT GGTGCCAACC TACAGCAGCT TAGATGCGGG GAAGATAGTT 
CTTGTAGACA CTATACTTAA ACTTGGAAAG GATTATGCAG GAGACACAGC AAGAACATTT 
TCACAGCATG CTGTGAATCA CCTCAAAGTT GCTTACATAC CACCAGCTAA AGACATTGGG 
CCAACTCCTC TACATGTCCA GTTTATATTA TCAGTGAGTA ACCAACATGG AGGAACAGTT 
CAAGGCATCT GTTTCAATAT CACTATCCTT CCAATAGACA ATCAGCCTCC TGAGGTGTTT 
TCATATGATT TGAAAGTTGA AGAAGGTGGT TTCAGCCAAA TTACTGCAAG TAACATTCAC 
ATCACAGATA AGGACACCAG ACAAGAACAA ATTAAAGTTA TTTTACACAA AGCTCCACTG 
CATGGTATCG TTCAACTTGA TGGATCTGCA TTGCAGGAAG GTGACCCATT CACCTGCTTG 
GACCTCATCA CATTAAAAGT TAGGTACCTT CATGATGGTT CAGAGACCCT ACAAGATGAA 
ATCTCATTTA TTGCAACAGA TGGAATCAAT TCATCTGAGT TTATTTTAAA AATACAGGTT 
TCTCCTATTA ATGATGAACC TCCATTTATT AAAGAGAACC TAATTTCCAC CATTATTTGC 
AAAGAAGGGG CAGAGGTAGT CATATCAGCA GACTATATCT CAGCTACTGA TGCAGACAGT 
GATGACATGA AGCTGATATT TATGATCGCT CGCCAACCAC GTTATGGCAT TTTGCTAAAG 
GCTGGATCTA TCATTGATCA ATTCACCCAG AGAGATGTTG TTGAAGGACT GGTACATTAT 
AGACATACAA GTGGCGAAAT TGGCTTGAAG GTCTATGTTG ATATTATAAC ACTGGTGGTT 
TCTGATGCTG AAGCAGATAA ACCGCAGAAA AAGTGCTGCA ACAATGGTCC TCGTCTGCCG 
ATTTTGCCAA ACCATGAGTC TTTTCCTGTA TATGATCTCA ATGTTACAAT TCTGCCAGTG 
GACAACCAAC CCCCATCGGT TATAATAGGT GAAATATTTG TAGTCGATGA GGGCAAATCA 
TCTGCCCTCA GTGTAGATCA TTTAAGTGCC ATGGACCCTG ACACAGAAAG TAACAATCTG 
GAATTCATAC TGGTTTCTCC TCCTCGATAT GGTTACATTG AAAATGTCCT CCCATCTCCT 
GGATTTGAGA AGAGTAATAT GGGTATAAGT ATTGCTGCAT TTCTACTCAA GGACATTAAA 
TCTCTGAACA TCAATTATGT CCAGTCCCGC CATCAAAGAA TTGAACCAAC CACTGATCAG 
TTTACACTGT ATGTCACTGA TGGCAAACTG CGTTCTGTAG AAAAGCCATT TTACATCGTA 
ATCAATCCGA CAAATGATGA GGTTCCGGAA TTTTTAGCCA GAAACATAAC AGTTGTTGAA 
GGACAAATGA AGAAATTGGA TCCCACTGTA ATAAATGTGT CAGACATGGA TGTGCCACAG 
AACCACTTAT ACTTTACTGT TATAGAGCAG CCCCGACATG GACTGATTAT TGAGGAATCG 
TATATCAGTG GGCACATGAA TAGAAGCCAA TTAATAAAAA GGCATCAGGC AAACAGCCTG 
CCTGTGATAC AAGATTTTTC CATGGACCTG CTAAAAAATG GAATGAAGAT GATATACATG 
CATGATGACT CAGAAAACCT TGCTGACAGC TTCACTATCC AGCTATCTGA TGGAAAGCAT 
GAAGTCAGGA GAACTATTTT TGTGAATGTA ATTGCAGTTA ATGACGAAAA GCCTGTGCTA 
ACCAAGCACT GCGATTTGGT GCTAAGCATG GGGGAACAAA CTCTTATATC AAGTGCCATA 
CTGTCAGCAG AAGATATGGA CACACCCAGG ACAAACATTT CCTATGTTAT TGACAGATGT 
CCAGAGCATG GCCAGCTTCA GATGAAAGTC AATAAAAACT GGGTGACCTT GCATACTGGA 
ATGAAATGCA CTCAGGAGGA CATTGATATG AACCTGGTTA GATATGTGCA TACTGGAACA 
ATGGGCTCAA AAAATGAAGA CACGTTTACT TTCCACCTTT GGGATGGCGG AAACCGATCA 
CCTGAGATAT CTGTTCATAT TTATGTCAAG GATATGGAAA AAGGAGATAT TGCAGTTTTT 
GTGAAAATGC TTAATGTGTC CAAGGGAGAG AGAGTTATCA TAACAACAAA TGTCCTTCTC 
GCCACTGATG GGACCGACAG ACCAGAAGAG CTGCTGTATG TGTTATCCTC GCCACCCCAA 
TATGGGCAGA TTGAATATGT TAAGCATCCT GGAATCCCCA TAACAAGTTT CAGTCAGATG 
GATGTGGCTG CTCACACAAT CTGCTACGTC CACGATTTCA ATTCAGCAGC CACTACTGAT 
ATGTTTAGAT TCCTAGTCAG CAATGGGCTG AAAACGAAAC ATGGGATCAT GAACATCGCA 
CTGGAAACAG TCGATCGAAC TCTTCCAACA CTATCCAGGA ACGATGGTCT AAGACAAGTG 
AGAGGGTCTA TGGAGTGTAT TTCCCCGGCC ACCCTACAGG CACTGGACCC TGACACGTCA 
TCTCAGAACA TTACTTTTCA GTTGGTGGGT CTCCCTCAAT ATGGGCAATT ATACCTCAGA 
GGCTTTCCAC TACAGCAAGA TCACTTCACC CAAAGAGATA TTGACAATCT AGACATTGCC 
TATAAGCATG CTGGACAGGA TGCTCAAATA GACAGGTTTA TGTTTACTGT TACAGACAAA 
GCAAATCAAG GATTTATCAT TAATGGGAAG ATTCAGACGG AACCAGTTCC ATTCACAATA 
CAGGTAGATC CTGTGGACAG CACAGCTCCC AGAATTGTCC ATCTTCAATG CCTTACAGAA 
GTTGAGCTTC TGAAAAATGG CCAGTATGGA ATTTATATTA CTTCAAGACA CTTAAAGGCA 
TCTGATCCAG ACACAGATGA TGAAAGAATT GTCTTTCACA TATTAGGAGG GCCTCACTAT 
GGTTATTTAG AGAATGTTAC AACAGGCAGG TTTATAAGAG AAAGATTTAC ACAAAGGGAT 
TTAAACAGCA AATCAGTGCT CTACGTAATA AATCCATCAG TGGATGTTTT TCATGACAAC 
ATAGAGTTTG AAGTTTCAGA CCCCGTTGGA AACAGAGCTT TACCACAAAT TTTAGAGCTG 
AAATGGAGTC AAATTGAATT GCTCCAAGGC ATGTATGAGG TGTGTGAAAC CGAGGCAGTT 
GTGTCTTTGA AGATGACAAG AAAAGGTTAT CTCCTGGAGT CTGCTTTTGT TTCGCTCAAG 
GTGAATGAAA TAAGTGCTGT TCTTGGCAAA GACATTTCCA TGAATCCTTC CAGACTTATA 
CAGTTTGATC CAGGAGTGTC AACCAAGACT TGGAATATAG TAATTATCCA GGATGGATTA 
GAAGAAGATG ACGAGGTCTT TGAAGTAGTT CTGAGTTCTC CTGTGAATGC TGTTCTTGGC 
TCTAAGACAA GAGCTGTAGT GAAAATTGTG GACCCCAGAG GAGGTGGCTG CAGTTCCTCC 
AAAGTCAGCA TGATCAAAAC AACTGGAATG GGCTTACTAA ACTGTACCGT CAAACCCTCA 
CGCAAACCTT GTGCTGTTTT TAGTTTTAAA GAGACTGCAG ATAAGGTCAG GGAAGATGTC 
CAAGAAGATG TCCATACCTT ACAACTTCCG AGGAAGAGAC TAAGACTCAC AGGGAACAGA 
AAAACAGTTA AACCATCATC AGTATTTAGA AACAAAACAG ACATTATTTA TCAATATCAT 
GGGATTGTGT CTCTCCGAGT GGAAGAAGAT AAGCCAGTAT TAAATGAAAC AAAGAAAGCA 
AAAGTACTAG TGAAGAACCG TGGTCAGAGG AAGATAAATG CCCCCTCCCA AAATCCTGAC 
ATTCTTCAAT CAGGCAGAAC CATGTTGGAT TCTCATGTTA CTACTGGTGG TCAGGATAAC 
GCAATATCGT TTCCTAAAAG ATGCACACCA AGTTTAAAAG GACTCCTGCA TTTTGAGGAC 
ACAGTACAGA AACTGTTCCA GTGTGACGGC ATATCGTGGA ACCCCTGGAT CACTACTAAG 
GATTCTAAAG CAGGAAAATG CCCATTTGGC TGGACTTACC ATGATGGCTA CTGCTATATA 
CTGATCAATG ATCAAAAGGC CACCTGGATA ACAGCAGCCA GAGCATGTAG AGAACAGCAT 
TTTGCTACTC TGGTGGATGT ATTCAGCAAG GAACAAATGG AATGGCTTTG GAATTTCAGT 
GGCAGAAAAT CCTTTTGGAT AGGCCTCAAT GATAGAGTAC GTAATGGAAA CTGGGAGTGG 
AGCAAAGGGG AAATGGTCAC CTTCACCAAT TGGAAAAGAG GCTCTCCTCG ATCTTCACAC 
AAAGGGAAGA ATTGCGTTTT GGCACAAAAG AGAGGCAAAT GGCAGGCTAA AGACTGCAAA 
AAAGGCAAAG GCCATTACTA TATATGTTCA AAAAAGTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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frem1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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