cacna1e cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following cacna1e gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cacna1e cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa44182 XM_018093597.1
Latest version!
Xenopus tropicalis calcium channel, voltage-dependent, R type, alpha 1E subunit (cacna1e), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OXa44183 XM_018093598.1
Latest version!
Xenopus tropicalis calcium channel, voltage-dependent, R type, alpha 1E subunit (cacna1e), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OXa57229 XM_031900979.1
Latest version!
Xenopus tropicalis calcium channel, voltage-dependent, R type, alpha 1E subunit (cacna1e), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa57230 XM_018093597.2
Latest version!
Xenopus tropicalis calcium channel, voltage-dependent, R type, alpha 1E subunit (cacna1e), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa57231 XM_031900980.1
Latest version!
Xenopus tropicalis calcium channel, voltage-dependent, R type, alpha 1E subunit (cacna1e), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa57232 XM_031900981.1
Latest version!
Xenopus tropicalis calcium channel, voltage-dependent, R type, alpha 1E subunit (cacna1e), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa57233 XM_031900982.1
Latest version!
Xenopus tropicalis calcium channel, voltage-dependent, R type, alpha 1E subunit (cacna1e), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa57234 XM_031900983.1
Latest version!
Xenopus tropicalis calcium channel, voltage-dependent, R type, alpha 1E subunit (cacna1e), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa44182
    Clone ID Related Accession (Same CDS sequence) XM_018093597.1
    Accession Version XM_018093597.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6435bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGATCCTTG CCACAATTAT TGCAAACTGC ATTGTCCTGG CTTTGGAGCA GCATCTTCCA 
    GGCGATGACA AGACTCCAAT GTCTCGCAGA TTGGAAAAGA CTGAGCCCTA TTTCATTGGG
    ATATTTTGCT TTGAAGCTGG AATTAAAATT GTCGCTCTGG GACTGGTTTT TCACAAAGGT
    TCTTATCTTC GGAATGGCTG GAATGTCATG GACTTTATAG TAGTGCTCAG TGGCATCCTG
    GCCACAGCTG GAACTCACTT CAATACTCAC GTGGACCTGC GTACTTTGCG GGCAGTGCGT
    GTTCTCAGGC CGTTGAAGCT GGTCTCGGGG ATTCCCAGTT TACAGATTGT GTTGAAGTCA
    ATCATGAAGG CGATGGTCCC TCTGCTACAG ATTGGATTGT TGCTCTTCTT TGCCATCCTT
    ATGTTTGCTA TCATCGGCCT TGAGTTTTAC AGTGGAAAGC TACACAAGGC CTGTTACAGG
    AACAACTCTG GAATGGAGCT AGACCCCATG GATCCACCTC ACCCTTGTGG AGTACCCCGC
    TGCCCAGCTG GGTATGAGTG CAAGGAATGG ATCGGACCCA ATGATGGAAT AACACAGTTT
    GACAACATCT TATTTGCGGT GCTGACAGTC TTCCAGTGTA TAACTATGGA GGGCTGGACC
    ACAGTGCTGT ACAATACCAA TGATGCCTTA GGAGCCACAT GGAACTGGCT GTACTTCATT
    CCCCTCATTA TCATCGGATC CTTCTTTGTC CTTAATCTTG TCCTTGGAGT GCTGTCCGGT
    GAATTTGCCA AAGAGAGGGA AAGAGTGGAA AACCGCCGGG CTTTCATGAA GTTGCGCAGG
    CAGCAGCAGA TTGAGAGGGA GTTGAATGGC TATCGGGCAT GGATAGATAA AGCTGAGGAA
    GTCATGTTGG CAGAAGAAAA CAAAAATTCT GGGACCTCTG CTTTAGAAGT TCTTAGGCGA
    GCAACCATTA AACGAAGTAG AACAGAAGCC ATGAACCGGG ACTCTACTGA GGACCATTGT
    GTTGACATAT CCTCTGTTGG CACCCCCCTT GCTAGAGCAA GCATCAAAAG TACCAAAATG
    GATGGGGCAT CATATTTTCG GCACAAGGAA AAACTATTGC GTGTCTCCGT TCGTCACATG
    ATCAAATCCC AGGTTTTCTA CTGGATGGTC CTGGGAGTGG TAGCACTTAA CACTTTCTGT
    GTGGCTATTG TCCATCACAA CCAGACCCTG TGGCTCTCAA ATGTTCTTTA CTATGCAGAA
    TTTCTGTTTC TTGGGTTATT TTTGGTGGAG ATGGCTTTTA AAATGTATGG AATGGGTCCA
    CGTCTTTACT TTCACTCATC CTTCAACTGC TTTGATTGCG GGGTCACAAT AGGTAGTATA
    TTTGAAGTAC TTTGGGCCAT ATTTAGACCA GGAACTTCCT TTGGAATCAG TGTCTTAAGA
    GCTCTCCGAC TTTTGAGAAT CTTCAAAATA ACAAAATACT GGGCCTCACT ACGAAACCTT
    GTAGTATCAT TGATGAATTC TATGAAATCC ATCATCAGCC TGCTCTTCCT TCTATTCCTC
    TTCATAGTTG TTTTTGCTCT CCTTGGAATG CAGTTATATG GTGGCAGATT CAATTTTAAT
    GATGGCACTC CTTCTGCAAA CTTTGATACA TTCCCTGCAG CCATCATGAC TGTATTCCAG
    ATTCTGACAG GAGAGGATTG GAATGAAGTG ATGTACAATG GTATTCGCTC CCAAGGTGGT
    GTTCGATCCG GAATGTGGTC TTCCATATAC TTTATTGTCC TTACCCTTTT TGGAAATTAC
    ACACTGCTAA ATGTATTTCT TGCCATTGCT GTGGACAACC TGGCTAATGC ACAGGAGTTA
    ACTAAGGATG AGCAAGAAGA AGAGGAAGCT TTCAATCAGA AGCATGCACT GCAAAAAGCG
    AAGGAGGTCA GCCCCATGTC TGCACCTAAT GCATCATGTA TTGAGAAGGA GCGCAGAAAA
    CGGCACACAT TGTCTATATG GGAGCAGCGC ACAAGCCAGC TAAGGAGGCA CATACAGATG
    TCTAGTCAAG AAGCTCTCCA GAGTGAAGAA CTGGCAGCAT TGAATCCACA CTCCAGTATC
    TATCGCAAGA CAAAGGAAGC TGCTGAACAA AACACAGAAA AAGAAGAAGT GCATCAAGAC
    GCAAAACTGG CAATAGACCC AGTTGAAGGA CCACCAGAAG GTGAAGGAAG CCCTTCTAAT
    AGAAATCTCA GGGAAAACTG GCGCCAGAAG TCCAGGCATG GAAATTCTGA AAACACTGAA
    CCAAATGGAA CAACTCCAGA AGAAGCAATG CGCCGCAGAC AAAGTCAGAG ACGCAGCCGT
    CATAGAAGAG TTAGAACTGA AGGTCGTGAG CAGTGTAGCA ATCCAAACAG TACCTCAGCA
    AGCAGAGAAG GTGGTCTTGA CAAAGTCTCT GCAGAGGATG GAAGAACAAT TCCAGAAGAG
    AAGAGCAATG GAGAAGCCAA AGAAGTCAAA GGGGTTGATG ATGAGCCTCT AAAGATTTGT
    ATATCTTCAA GAAATTGCGA GGACAGAAAT ATCAGGACTT TGGGTTGTGA GGGTATTGCC
    TTGAACGATA CACCCCTGCA GGATGTGAAC AGCCAAGAGA AGGACAGTAG CCCTCCTGAT
    TTGGATTGCA GTGAGCAGGC TCTGTTGGGC AGCATTCAGA GCCAGGAAAG CCCTAATCCA
    ACCAGCTGCA GTTTGCCAGA TGTGTCCAGC AGCACCGAGA GAGTCACAGA GAGCACCGTC
    ATTACCTTAC CAGATACTGG AATGCCCACC GACTGCACCG TGGTACAGAT GAGTGAAGGC
    AGGGATGGAG AAGTCAACTT TGGGTCTTCG CAGCAAGAAA AAGAAGAGGA GGAAGCTGAG
    GATGGAGAAG AAAGAACTAT AAAAAGAAAA GAGAAATTGT TGGAGTCCCC CAAAGCCATT
    GTACCGTACA GTTCAATGCT CATCTTCAGC TCCACCAATC CCATAAGAAA AGTATGTCAT
    TACATTGTTA ACCTACGATA CTTTGAGATG AGCATCCTGC TGGTAATAGC AGCAAGTAGT
    ATAGCCTTAG CAGCGGAGGA CCCTGTACTG ACCAACTCGG ACCGCAACAA AGTTCTTCGC
    TATTTTGACT ATGTTTTCAC AGGGGTCTTC ACTTTTGAGA TGATTATAAA GATGATTGAC
    CAAGGTTTGA TTTTACACGA GGGCTCCTAC TTTCGTGACC TATGGAACAT CTTGGATTTT
    GTTGTTGTTG TGGGAGCCTT AATTGCGTTT GCCTTAGCGA ATGTTTTGGG GACTAACAAA
    GGCCGGGACA TCAAAACCAT TAAGTCTCTA CGTGTGTTAC GGGTGCTGCG TCCACTAAAA
    ACAATCAAAC GCCTGCCAAA ACTGAAGGCA GTCTTTGATT GTGTGGTTAA CTCTTTAAAG
    AATGTCTTTA ATATTCTCAT CGTATACAAA CTCTTCATGT TCATATTTGC TGTAATCGCT
    GTCCAGCTGT TTAAGGGCAA GTTCTTCTAT TGTACAGACA GCTCAAAGGA GACAGAGAAA
    GAGTGCATAG GGAGTTACAT CGATCACGAA AAGAACAAGA TGGAGGTGAA GCGGAGAGAG
    TGGAAAAGAC ATGATTTCCA TTATGACAAC ATAATATGGG CTCTTCTTAC CCTCTTTACT
    GTATCAACAG GGGAAGGCTG GCCTCAGGTT CTGCAGCACT CTGTTGATAT AACTGAAGAA
    GATAGGGGTC CAAGCCGAAG CAACCGTATG GAGATGTCCA TTTTCTATGT TATCTACTTT
    GTGGTCTTCC CCTTCTTCTT TGTAAATATA TTTGTGGCTC TGATTATCAT CACATTCCAG
    GAGCAAGGAG ACAAGATGAT GGAGGAATGT TCCCTAGAAA AGAATGAGAG AGCCTGCATA
    GACTTTGCAA TAAGTGCTAA GCCACTTACA CGATATATGC CACAGAACAG GCACACATTC
    CAGTACCGGG TCTGGCACTT CGTGGTCTCC CCATCATTTG AATACACTAT CATGGCTATG
    ATTGCACTAA ACACAGTGGT ACTCATGATG AAGTATTATT CTGCGCCATA CACATATGAG
    CTTGCATTAA AGTATCTCAA TATTGCATTC ACAATGCTAT TTTCTCTGGA GTGCATCCTG
    AAAATAATTG CATTTGGGTT TTTGAATTAC TTTCGGGATA CTTGGAACAT TTTTGACTTC
    ATTACTGTAA TTGGTAGCAT CACGGAAATT ATTCTAACAG ATAGTAAGTT GGTGAACACC
    AGCAGCTTTA ATATGAGCTT TCTGAAGCTG TTCCGAGCGG CGCGACTTAT TAAACTTCTC
    CGGCAAGGGT ACACCATCCG GATCCTGCTG TGGACTTTTG TTCAGTCCTT TAAGGCACTT
    CCTTATGTGT GTCTTCTCAT TGCAATGTTG TTCTTCATCT ATGCCATTAT TGGGATGCAG
    GTGTTTGGCA ATATCCGCCT GGAAGAGGAC AGTCATATTA ATAGGCACAA CAATTTTAGA
    AGCTTCTTTA GTTCTCTTAT GTTACTCTTC AGGAGTGCCA CAGGAGAAGC GTGGCAGGAG
    ATCATGCTTT CATGCCTTAG TGATAAGGGT TGTGAGCCAG ACACCAGTGC AGCCTCTGGA
    CACAATGAGA ATGAGCGCTG TGGAACTGAA CTTGCATATG TTTATTTTGT TTCATTCATT
    TTCTTCTGCT CCTTCCTCAT GCTAAATCTG TTTGTGGCTG TCATTATGGA CAACTTTGAG
    TACTTGACAC GGGACTCTTC CATTTTGGGA CCTCATCACT TGGATGAGTT TGTTCGGATA
    TGGGCTGAAT ATGATCGAGC AGCATGTGGC AGGATCCCCT ATAAGGATAT GTACAAATTA
    GTCCGAGTCA TCTCGCCTCC GCTCGGCCTA GGAGAGAACT GCCCATACAG GGTGGCATGC
    AAGAGATTGG TCCTGATGAA TATGCCAGTG TCGGAGGACA TGACGGTTCA TTTCACATCA
    ACTCTTATGG CCCTAATACG CACGGCACTG GATATCAAAA TAGCTAAAGG AGGTGCTGAC
    AGGCAACAGC TGGACTCAGA GCTGCAGAAA GATATACTTG CCATTTGGCC AAATCTCTCC
    CAGAAAATGA TCGACTTACT TGTGCCAATG CCAAGAGCCT CTGATCTGAC GGTGGGGAAG
    ATTTACGCTG CAATGATGAT CATGGATTAC TATAAACAGA GCAAAGCGAA AAAACAAAGG
    CAGCAGCTGG AGGAGCAGAA AAATGCACCT ATGTTTCAGA GAATGGAGGC TTCTGCCCTC
    CCTGAGGAGA TCTTATCCAA TGCCAAAGCT TTACCATACC TTCAGCAAGA CACTCTATCA
    GGCCTGGGTG GATTTCCTTC ACTGAGTCCC TTGTCCCCAG AAGAAGTATT CCAGCCGCCA
    TGCCTAGACG GGGAAGAGGA GGAAGACTTT GAAGAGCAGC TGTCTCTGGA GCCTGAGGTT
    AGAGAGTTTA GAAGGGAGCA GCTCCCTAAC CGAGGCAGCT ACATCCCTGT GGAGATGCTG
    GAACGTCCAG CGTCTGTGAG AGCTTCTTCC ATGCCACGTC TGGCTGAGAA TCCTCAGGTC
    CTGTCAGACG TAAGCTCCAT GCGTCGCTCC TTCTCCACAA CTGCAGAGAA GCGAGTGCGC
    AGCTCGTGGC TAGATGAGTT CACTATGGAC AGGAGTAGCA CTGACTGTGC ATACAGATAC
    CAGAGGCGCA GTTATCATGC ATCCTTAAGA CTGTCTGCAC ATCGCCTGAA TACAGATACA
    GGTCACTGGT CAGATAATCA CCGTTCTGGC GGAAGGGAGC GAGGAAGGTC CAAAGAGAGA
    AAACACTTGC TGTCACCCGA TCCTTCCCGC TGTAATTCTG AGGAAAGGAG CCCACAAGCT
    GCCTGTGAAC ACCGAGATCA CCAACTGTCC CCTGGGCATA CTCCCAGTAG TGTACAGATT
    CTTGACTCTC TGAGTGAAAG TTGTACCCCT TCAGACACCA GCACCCCAAG AAGAGGCCGT
    AGACAGCTTC CTTCTGTGCC TCCAACTCCT AGACCCCTTA TCTCTTTCTC CTCGTTAATC
    CGCCGGCAGA AACACAGCCC CCCTCCTCAA AATAAAAATG ATACTATCCT TCCTAGTGAG
    CCTGGTCTGA CTGAGTCTTC CTGCTCCCTT GGGGGTCATC AGAGCTCCCA GTCCACACCA
    CAGCATTATA TCTCAGAGCC CTACCTGGCA CTGCATGAGG AGATGCAAAG CTCAGACTGC
    CCAGCAGAGG ACACACTCAC CTTTGAGGTT GCTGTGGCTA CTAGTCTGGG CCGCTCGAAC
    ACCATTGGCT CTGCCCCTCT ACGTCATGGC TGGCAAATGC CCAATGGTCA CTACCGGCGT
    CGAAGGAAGG GAGGAATTCC ACCAGTGATA CCCTCCAGAG AGTTAAGTGA CACAGAAGAG
    GATGATCGAT GTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017949086.1
    CDS9..6443
    Translation

    Target ORF information:

    RefSeq Version XM_018093597.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calcium channel, voltage-dependent, R type, alpha 1E subunit (cacna1e), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018093597.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGATCCTTG CCACAATTAT TGCAAACTGC ATTGTCCTGG CTTTGGAGCA GCATCTTCCA 
    GGCGATGACA AGACTCCAAT GTCTCGCAGA TTGGAAAAGA CTGAGCCCTA TTTCATTGGG
    ATATTTTGCT TTGAAGCTGG AATTAAAATT GTCGCTCTGG GACTGGTTTT TCACAAAGGT
    TCTTATCTTC GGAATGGCTG GAATGTCATG GACTTTATAG TAGTGCTCAG TGGCATCCTG
    GCCACAGCTG GAACTCACTT CAATACTCAC GTGGACCTGC GTACTTTGCG GGCAGTGCGT
    GTTCTCAGGC CGTTGAAGCT GGTCTCGGGG ATTCCCAGTT TACAGATTGT GTTGAAGTCA
    ATCATGAAGG CGATGGTCCC TCTGCTACAG ATTGGATTGT TGCTCTTCTT TGCCATCCTT
    ATGTTTGCTA TCATCGGCCT TGAGTTTTAC AGTGGAAAGC TACACAAGGC CTGTTACAGG
    AACAACTCTG GAATGGAGCT AGACCCCATG GATCCACCTC ACCCTTGTGG AGTACCCCGC
    TGCCCAGCTG GGTATGAGTG CAAGGAATGG ATCGGACCCA ATGATGGAAT AACACAGTTT
    GACAACATCT TATTTGCGGT GCTGACAGTC TTCCAGTGTA TAACTATGGA GGGCTGGACC
    ACAGTGCTGT ACAATACCAA TGATGCCTTA GGAGCCACAT GGAACTGGCT GTACTTCATT
    CCCCTCATTA TCATCGGATC CTTCTTTGTC CTTAATCTTG TCCTTGGAGT GCTGTCCGGT
    GAATTTGCCA AAGAGAGGGA AAGAGTGGAA AACCGCCGGG CTTTCATGAA GTTGCGCAGG
    CAGCAGCAGA TTGAGAGGGA GTTGAATGGC TATCGGGCAT GGATAGATAA AGCTGAGGAA
    GTCATGTTGG CAGAAGAAAA CAAAAATTCT GGGACCTCTG CTTTAGAAGT TCTTAGGCGA
    GCAACCATTA AACGAAGTAG AACAGAAGCC ATGAACCGGG ACTCTACTGA GGACCATTGT
    GTTGACATAT CCTCTGTTGG CACCCCCCTT GCTAGAGCAA GCATCAAAAG TACCAAAATG
    GATGGGGCAT CATATTTTCG GCACAAGGAA AAACTATTGC GTGTCTCCGT TCGTCACATG
    ATCAAATCCC AGGTTTTCTA CTGGATGGTC CTGGGAGTGG TAGCACTTAA CACTTTCTGT
    GTGGCTATTG TCCATCACAA CCAGACCCTG TGGCTCTCAA ATGTTCTTTA CTATGCAGAA
    TTTCTGTTTC TTGGGTTATT TTTGGTGGAG ATGGCTTTTA AAATGTATGG AATGGGTCCA
    CGTCTTTACT TTCACTCATC CTTCAACTGC TTTGATTGCG GGGTCACAAT AGGTAGTATA
    TTTGAAGTAC TTTGGGCCAT ATTTAGACCA GGAACTTCCT TTGGAATCAG TGTCTTAAGA
    GCTCTCCGAC TTTTGAGAAT CTTCAAAATA ACAAAATACT GGGCCTCACT ACGAAACCTT
    GTAGTATCAT TGATGAATTC TATGAAATCC ATCATCAGCC TGCTCTTCCT TCTATTCCTC
    TTCATAGTTG TTTTTGCTCT CCTTGGAATG CAGTTATATG GTGGCAGATT CAATTTTAAT
    GATGGCACTC CTTCTGCAAA CTTTGATACA TTCCCTGCAG CCATCATGAC TGTATTCCAG
    ATTCTGACAG GAGAGGATTG GAATGAAGTG ATGTACAATG GTATTCGCTC CCAAGGTGGT
    GTTCGATCCG GAATGTGGTC TTCCATATAC TTTATTGTCC TTACCCTTTT TGGAAATTAC
    ACACTGCTAA ATGTATTTCT TGCCATTGCT GTGGACAACC TGGCTAATGC ACAGGAGTTA
    ACTAAGGATG AGCAAGAAGA AGAGGAAGCT TTCAATCAGA AGCATGCACT GCAAAAAGCG
    AAGGAGGTCA GCCCCATGTC TGCACCTAAT GCATCATGTA TTGAGAAGGA GCGCAGAAAA
    CGGCACACAT TGTCTATATG GGAGCAGCGC ACAAGCCAGC TAAGGAGGCA CATACAGATG
    TCTAGTCAAG AAGCTCTCCA GAGTGAAGAA CTGGCAGCAT TGAATCCACA CTCCAGTATC
    TATCGCAAGA CAAAGGAAGC TGCTGAACAA AACACAGAAA AAGAAGAAGT GCATCAAGAC
    GCAAAACTGG CAATAGACCC AGTTGAAGGA CCACCAGAAG GTGAAGGAAG CCCTTCTAAT
    AGAAATCTCA GGGAAAACTG GCGCCAGAAG TCCAGGCATG GAAATTCTGA AAACACTGAA
    CCAAATGGAA CAACTCCAGA AGAAGCAATG CGCCGCAGAC AAAGTCAGAG ACGCAGCCGT
    CATAGAAGAG TTAGAACTGA AGGTCGTGAG CAGTGTAGCA ATCCAAACAG TACCTCAGCA
    AGCAGAGAAG GTGGTCTTGA CAAAGTCTCT GCAGAGGATG GAAGAACAAT TCCAGAAGAG
    AAGAGCAATG GAGAAGCCAA AGAAGTCAAA GGGGTTGATG ATGAGCCTCT AAAGATTTGT
    ATATCTTCAA GAAATTGCGA GGACAGAAAT ATCAGGACTT TGGGTTGTGA GGGTATTGCC
    TTGAACGATA CACCCCTGCA GGATGTGAAC AGCCAAGAGA AGGACAGTAG CCCTCCTGAT
    TTGGATTGCA GTGAGCAGGC TCTGTTGGGC AGCATTCAGA GCCAGGAAAG CCCTAATCCA
    ACCAGCTGCA GTTTGCCAGA TGTGTCCAGC AGCACCGAGA GAGTCACAGA GAGCACCGTC
    ATTACCTTAC CAGATACTGG AATGCCCACC GACTGCACCG TGGTACAGAT GAGTGAAGGC
    AGGGATGGAG AAGTCAACTT TGGGTCTTCG CAGCAAGAAA AAGAAGAGGA GGAAGCTGAG
    GATGGAGAAG AAAGAACTAT AAAAAGAAAA GAGAAATTGT TGGAGTCCCC CAAAGCCATT
    GTACCGTACA GTTCAATGCT CATCTTCAGC TCCACCAATC CCATAAGAAA AGTATGTCAT
    TACATTGTTA ACCTACGATA CTTTGAGATG AGCATCCTGC TGGTAATAGC AGCAAGTAGT
    ATAGCCTTAG CAGCGGAGGA CCCTGTACTG ACCAACTCGG ACCGCAACAA AGTTCTTCGC
    TATTTTGACT ATGTTTTCAC AGGGGTCTTC ACTTTTGAGA TGATTATAAA GATGATTGAC
    CAAGGTTTGA TTTTACACGA GGGCTCCTAC TTTCGTGACC TATGGAACAT CTTGGATTTT
    GTTGTTGTTG TGGGAGCCTT AATTGCGTTT GCCTTAGCGA ATGTTTTGGG GACTAACAAA
    GGCCGGGACA TCAAAACCAT TAAGTCTCTA CGTGTGTTAC GGGTGCTGCG TCCACTAAAA
    ACAATCAAAC GCCTGCCAAA ACTGAAGGCA GTCTTTGATT GTGTGGTTAA CTCTTTAAAG
    AATGTCTTTA ATATTCTCAT CGTATACAAA CTCTTCATGT TCATATTTGC TGTAATCGCT
    GTCCAGCTGT TTAAGGGCAA GTTCTTCTAT TGTACAGACA GCTCAAAGGA GACAGAGAAA
    GAGTGCATAG GGAGTTACAT CGATCACGAA AAGAACAAGA TGGAGGTGAA GCGGAGAGAG
    TGGAAAAGAC ATGATTTCCA TTATGACAAC ATAATATGGG CTCTTCTTAC CCTCTTTACT
    GTATCAACAG GGGAAGGCTG GCCTCAGGTT CTGCAGCACT CTGTTGATAT AACTGAAGAA
    GATAGGGGTC CAAGCCGAAG CAACCGTATG GAGATGTCCA TTTTCTATGT TATCTACTTT
    GTGGTCTTCC CCTTCTTCTT TGTAAATATA TTTGTGGCTC TGATTATCAT CACATTCCAG
    GAGCAAGGAG ACAAGATGAT GGAGGAATGT TCCCTAGAAA AGAATGAGAG AGCCTGCATA
    GACTTTGCAA TAAGTGCTAA GCCACTTACA CGATATATGC CACAGAACAG GCACACATTC
    CAGTACCGGG TCTGGCACTT CGTGGTCTCC CCATCATTTG AATACACTAT CATGGCTATG
    ATTGCACTAA ACACAGTGGT ACTCATGATG AAGTATTATT CTGCGCCATA CACATATGAG
    CTTGCATTAA AGTATCTCAA TATTGCATTC ACAATGCTAT TTTCTCTGGA GTGCATCCTG
    AAAATAATTG CATTTGGGTT TTTGAATTAC TTTCGGGATA CTTGGAACAT TTTTGACTTC
    ATTACTGTAA TTGGTAGCAT CACGGAAATT ATTCTAACAG ATAGTAAGTT GGTGAACACC
    AGCAGCTTTA ATATGAGCTT TCTGAAGCTG TTCCGAGCGG CGCGACTTAT TAAACTTCTC
    CGGCAAGGGT ACACCATCCG GATCCTGCTG TGGACTTTTG TTCAGTCCTT TAAGGCACTT
    CCTTATGTGT GTCTTCTCAT TGCAATGTTG TTCTTCATCT ATGCCATTAT TGGGATGCAG
    GTGTTTGGCA ATATCCGCCT GGAAGAGGAC AGTCATATTA ATAGGCACAA CAATTTTAGA
    AGCTTCTTTA GTTCTCTTAT GTTACTCTTC AGGAGTGCCA CAGGAGAAGC GTGGCAGGAG
    ATCATGCTTT CATGCCTTAG TGATAAGGGT TGTGAGCCAG ACACCAGTGC AGCCTCTGGA
    CACAATGAGA ATGAGCGCTG TGGAACTGAA CTTGCATATG TTTATTTTGT TTCATTCATT
    TTCTTCTGCT CCTTCCTCAT GCTAAATCTG TTTGTGGCTG TCATTATGGA CAACTTTGAG
    TACTTGACAC GGGACTCTTC CATTTTGGGA CCTCATCACT TGGATGAGTT TGTTCGGATA
    TGGGCTGAAT ATGATCGAGC AGCATGTGGC AGGATCCCCT ATAAGGATAT GTACAAATTA
    GTCCGAGTCA TCTCGCCTCC GCTCGGCCTA GGAGAGAACT GCCCATACAG GGTGGCATGC
    AAGAGATTGG TCCTGATGAA TATGCCAGTG TCGGAGGACA TGACGGTTCA TTTCACATCA
    ACTCTTATGG CCCTAATACG CACGGCACTG GATATCAAAA TAGCTAAAGG AGGTGCTGAC
    AGGCAACAGC TGGACTCAGA GCTGCAGAAA GATATACTTG CCATTTGGCC AAATCTCTCC
    CAGAAAATGA TCGACTTACT TGTGCCAATG CCAAGAGCCT CTGATCTGAC GGTGGGGAAG
    ATTTACGCTG CAATGATGAT CATGGATTAC TATAAACAGA GCAAAGCGAA AAAACAAAGG
    CAGCAGCTGG AGGAGCAGAA AAATGCACCT ATGTTTCAGA GAATGGAGGC TTCTGCCCTC
    CCTGAGGAGA TCTTATCCAA TGCCAAAGCT TTACCATACC TTCAGCAAGA CACTCTATCA
    GGCCTGGGTG GATTTCCTTC ACTGAGTCCC TTGTCCCCAG AAGAAGTATT CCAGCCGCCA
    TGCCTAGACG GGGAAGAGGA GGAAGACTTT GAAGAGCAGC TGTCTCTGGA GCCTGAGGTT
    AGAGAGTTTA GAAGGGAGCA GCTCCCTAAC CGAGGCAGCT ACATCCCTGT GGAGATGCTG
    GAACGTCCAG CGTCTGTGAG AGCTTCTTCC ATGCCACGTC TGGCTGAGAA TCCTCAGGTC
    CTGTCAGACG TAAGCTCCAT GCGTCGCTCC TTCTCCACAA CTGCAGAGAA GCGAGTGCGC
    AGCTCGTGGC TAGATGAGTT CACTATGGAC AGGAGTAGCA CTGACTGTGC ATACAGATAC
    CAGAGGCGCA GTTATCATGC ATCCTTAAGA CTGTCTGCAC ATCGCCTGAA TACAGATACA
    GGTCACTGGT CAGATAATCA CCGTTCTGGC GGAAGGGAGC GAGGAAGGTC CAAAGAGAGA
    AAACACTTGC TGTCACCCGA TCCTTCCCGC TGTAATTCTG AGGAAAGGAG CCCACAAGCT
    GCCTGTGAAC ACCGAGATCA CCAACTGTCC CCTGGGCATA CTCCCAGTAG TGTACAGATT
    CTTGACTCTC TGAGTGAAAG TTGTACCCCT TCAGACACCA GCACCCCAAG AAGAGGCCGT
    AGACAGCTTC CTTCTGTGCC TCCAACTCCT AGACCCCTTA TCTCTTTCTC CTCGTTAATC
    CGCCGGCAGA AACACAGCCC CCCTCCTCAA AATAAAAATG ATACTATCCT TCCTAGTGAG
    CCTGGTCTGA CTGAGTCTTC CTGCTCCCTT GGGGGTCATC AGAGCTCCCA GTCCACACCA
    CAGCATTATA TCTCAGAGCC CTACCTGGCA CTGCATGAGG AGATGCAAAG CTCAGACTGC
    CCAGCAGAGG ACACACTCAC CTTTGAGGTT GCTGTGGCTA CTAGTCTGGG CCGCTCGAAC
    ACCATTGGCT CTGCCCCTCT ACGTCATGGC TGGCAAATGC CCAATGGTCA CTACCGGCGT
    CGAAGGAAGG GAGGAATTCC ACCAGTGATA CCCTCCAGAG AGTTAAGTGA CACAGAAGAG
    GATGATCGAT GTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa44183
    Clone ID Related Accession (Same CDS sequence) XM_018093598.1
    Accession Version XM_018093598.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6423bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGATCCTTG CCACAATTAT TGCAAACTGC ATTGTCCTGG CTTTGGAGCA GCATCTTCCA 
    GGCGATGACA AGACTCCAAT GTCTCGCAGA TTGGAAAAGA CTGAGCCCTA TTTCATTGGG
    ATATTTTGCT TTGAAGCTGG AATTAAAATT GTCGCTCTGG GACTGGTTTT TCACAAAGGT
    TCTTATCTTC GGAATGGCTG GAATGTCATG GACTTTATAG TAGTGCTCAG TGGCATCCTG
    GCCACAGCTG GAACTCACTT CAATACTCAC GTGGACCTGC GTACTTTGCG GGCAGTGCGT
    GTTCTCAGGC CGTTGAAGCT GGTCTCGGGG ATTCCCAGTT TACAGATTGT GTTGAAGTCA
    ATCATGAAGG CGATGGTCCC TCTGCTACAG ATTGGATTGT TGCTCTTCTT TGCCATCCTT
    ATGTTTGCTA TCATCGGCCT TGAGTTTTAC AGTGGAAAGC TACACAAGGC CTGTTACAGG
    AACAACTCTG GAATGGAGCT AGACCCCATG GATCCACCTC ACCCTTGTGG AGTACCCCGC
    TGCCCAGCTG GGTATGAGTG CAAGGAATGG ATCGGACCCA ATGATGGAAT AACACAGTTT
    GACAACATCT TATTTGCGGT GCTGACAGTC TTCCAGTGTA TAACTATGGA GGGCTGGACC
    ACAGTGCTGT ACAATACCAA TGATGCCTTA GGAGCCACAT GGAACTGGCT GTACTTCATT
    CCCCTCATTA TCATCGGATC CTTCTTTGTC CTTAATCTTG TCCTTGGAGT GCTGTCCGGT
    GAATTTGCCA AAGAGAGGGA AAGAGTGGAA AACCGCCGGG CTTTCATGAA GTTGCGCAGG
    CAGCAGCAGA TTGAGAGGGA GTTGAATGGC TATCGGGCAT GGATAGATAA AGCTGAGGAA
    GTCATGTTGG CAGAAGAAAA CAAAAATTCT GGGACCTCTG CTTTAGAAGT TCTTAGGCGA
    GCAACCATTA AACGAAGTAG AACAGAAGCC ATGAACCGGG ACTCTACTGA GGACCATTGT
    GTTGACATAT CCTCTGTTGG CACCCCCCTT GCTAGAGCAA GCATCAAAAG TACCAAAATG
    GATGGGGCAT CATATTTTCG GCACAAGGAA AAACTATTGC GTGTCTCCGT TCGTCACATG
    ATCAAATCCC AGGTTTTCTA CTGGATGGTC CTGGGAGTGG TAGCACTTAA CACTTTCTGT
    GTGGCTATTG TCCATCACAA CCAGACCCTG TGGCTCTCAA ATGTTCTTTA CTATGCAGAA
    TTTCTGTTTC TTGGGTTATT TTTGGTGGAG ATGGCTTTTA AAATGTATGG AATGGGTCCA
    CGTCTTTACT TTCACTCATC CTTCAACTGC TTTGATTGCG GGGTCACAAT AGGTAGTATA
    TTTGAAGTAC TTTGGGCCAT ATTTAGACCA GGAACTTCCT TTGGAATCAG TGTCTTAAGA
    GCTCTCCGAC TTTTGAGAAT CTTCAAAATA ACAAAATACT GGGCCTCACT ACGAAACCTT
    GTAGTATCAT TGATGAATTC TATGAAATCC ATCATCAGCC TGCTCTTCCT TCTATTCCTC
    TTCATAGTTG TTTTTGCTCT CCTTGGAATG CAGTTATATG GTGGCAGATT CAATTTTAAT
    GATGGCACTC CTTCTGCAAA CTTTGATACA TTCCCTGCAG CCATCATGAC TGTATTCCAG
    ATTCTGACAG GAGAGGATTG GAATGAAGTG ATGTACAATG GTATTCGCTC CCAAGGTGGT
    GTTCGATCCG GAATGTGGTC TTCCATATAC TTTATTGTCC TTACCCTTTT TGGAAATTAC
    ACACTGCTAA ATGTATTTCT TGCCATTGCT GTGGACAACC TGGCTAATGC ACAGGAGTTA
    ACTAAGGATG AGCAAGAAGA AGAGGAAGCT TTCAATCAGA AGCATGCACT GCAAAAAGCG
    AAGGAGGTCA GCCCCATGTC TGCACCTAAT GCATCATGTA TTGAGAAGGA GCGCAGAAAA
    CGGCACACAT TGTCTATATG GGAGCAGCGC ACAAGCCAGC TAAGGAGGCA CATACAGATG
    TCTAGTCAAG AAGCTCTCCA GAGTGAAGAA CTGGCAGCAT TGAATCCACA CTCCAGTATC
    TATCGCAAGA CAAAGGAAGC TGCTGAACAA AACACAGAAA AAGAAGAAGT GCATCAAGAC
    GCAAAACTGG CAATAGACCC AGTTGAAGGA CCACCAGAAG GTGAAGGAAG CCCTTCTAAT
    AGAAATCTCA GGGAAAACTG GCGCCAGAAG TCCAGGCATG GAAATTCTGA AAACACTGAA
    CCAAATGGAA CAACTCCAGA AGAAGCAATG CGCCGCAGAC AAAGTCAGAG ACGCAGCCGT
    CATAGAAGAG TTAGAACTGA AGGTCGTGAG CAGTGTAGCA ATCCAAACAG TACCTCAGCA
    AGCAGAGAAG GTGGTCTTGA CAAAGTCTCT GCAGAGGATG GAAGAACAAT TCCAGAAGAG
    AAGAGCAATG GAGAAGCCAA AGAAGTCAAA GGGGTTGATG ATGAGCCTCT AAAGATTTGT
    ATATCTTCAA GAAATTGCGA GGACAGAAAT ATCAGGACTT TGGGTTGTGA GGGTATTGCC
    TTGAACGATA CACCCCTGCA GGATGTGAAC AGCCAAGAGA AGGACAGTAG CCCTCCTGAT
    TTGGATTGCA GTGAGCAGGC TCTGTTGGGC AGCATTCAGA GCCAGGAAAG CCCTAATCCA
    ACCAGCTGCA GTTTGCCAGA TGTGTCCAGC AGCACCGAGA GAGTCACAGA GAGCACCGTC
    ATTACCTTAC CAGATACTGG AATGCCCACC GACTGCACCG TGGTACAGAT GAGTGAAGGC
    AGGGATGGAG AAGTCAACTT TGGGTCTTCG CAGCAAGAAA AAGAAGAGGA GGAAGCTGAG
    GATGGAGAAG AAAGAACTAT AAAAAGAAAA GAGAAATTGT TGGAGTCCCC CAAAGCCATT
    GTACCGTACA GTTCAATGCT CATCTTCAGC TCCACCAATC CCATAAGAAA AGTATGTCAT
    TACATTGTTA ACCTACGATA CTTTGAGATG AGCATCCTGC TGGTAATAGC AGCAAGTAGT
    ATAGCCTTAG CAGCGGAGGA CCCTGTACTG ACCAACTCGG ACCGCAACAA AGTTCTTCGC
    TATTTTGACT ATGTTTTCAC AGGGGTCTTC ACTTTTGAGA TGATTATAAA GATGATTGAC
    CAAGGTTTGA TTTTACACGA GGGCTCCTAC TTTCGTGACC TATGGAACAT CTTGGATTTT
    GTTGTTGTTG TGGGAGCCTT AATTGCGTTT GCCTTAGCGA CTAACAAAGG CCGGGACATC
    AAAACCATTA AGTCTCTACG TGTGTTACGG GTGCTGCGTC CACTAAAAAC AATCAAACGC
    CTGCCAAAAC TGAAGGCAGT CTTTGATTGT GTGGTTAACT CTTTAAAGAA TGTCTTTAAT
    ATTCTCATCG TATACAAACT CTTCATGTTC ATATTTGCTG TAATCGCTGT CCAGCTGTTT
    AAGGGCAAGT TCTTCTATTG TACAGACAGC TCAAAGGAGA CAGAGAAAGA GTGCATAGGG
    AGTTACATCG ATCACGAAAA GAACAAGATG GAGGTGAAGC GGAGAGAGTG GAAAAGACAT
    GATTTCCATT ATGACAACAT AATATGGGCT CTTCTTACCC TCTTTACTGT ATCAACAGGG
    GAAGGCTGGC CTCAGGTTCT GCAGCACTCT GTTGATATAA CTGAAGAAGA TAGGGGTCCA
    AGCCGAAGCA ACCGTATGGA GATGTCCATT TTCTATGTTA TCTACTTTGT GGTCTTCCCC
    TTCTTCTTTG TAAATATATT TGTGGCTCTG ATTATCATCA CATTCCAGGA GCAAGGAGAC
    AAGATGATGG AGGAATGTTC CCTAGAAAAG AATGAGAGAG CCTGCATAGA CTTTGCAATA
    AGTGCTAAGC CACTTACACG ATATATGCCA CAGAACAGGC ACACATTCCA GTACCGGGTC
    TGGCACTTCG TGGTCTCCCC ATCATTTGAA TACACTATCA TGGCTATGAT TGCACTAAAC
    ACAGTGGTAC TCATGATGAA GTATTATTCT GCGCCATACA CATATGAGCT TGCATTAAAG
    TATCTCAATA TTGCATTCAC AATGCTATTT TCTCTGGAGT GCATCCTGAA AATAATTGCA
    TTTGGGTTTT TGAATTACTT TCGGGATACT TGGAACATTT TTGACTTCAT TACTGTAATT
    GGTAGCATCA CGGAAATTAT TCTAACAGAT AGTAAGTTGG TGAACACCAG CAGCTTTAAT
    ATGAGCTTTC TGAAGCTGTT CCGAGCGGCG CGACTTATTA AACTTCTCCG GCAAGGGTAC
    ACCATCCGGA TCCTGCTGTG GACTTTTGTT CAGTCCTTTA AGGCACTTCC TTATGTGTGT
    CTTCTCATTG CAATGTTGTT CTTCATCTAT GCCATTATTG GGATGCAGGT GTTTGGCAAT
    ATCCGCCTGG AAGAGGACAG TCATATTAAT AGGCACAACA ATTTTAGAAG CTTCTTTAGT
    TCTCTTATGT TACTCTTCAG GAGTGCCACA GGAGAAGCGT GGCAGGAGAT CATGCTTTCA
    TGCCTTAGTG ATAAGGGTTG TGAGCCAGAC ACCAGTGCAG CCTCTGGACA CAATGAGAAT
    GAGCGCTGTG GAACTGAACT TGCATATGTT TATTTTGTTT CATTCATTTT CTTCTGCTCC
    TTCCTCATGC TAAATCTGTT TGTGGCTGTC ATTATGGACA ACTTTGAGTA CTTGACACGG
    GACTCTTCCA TTTTGGGACC TCATCACTTG GATGAGTTTG TTCGGATATG GGCTGAATAT
    GATCGAGCAG CATGTGGCAG GATCCCCTAT AAGGATATGT ACAAATTAGT CCGAGTCATC
    TCGCCTCCGC TCGGCCTAGG AGAGAACTGC CCATACAGGG TGGCATGCAA GAGATTGGTC
    CTGATGAATA TGCCAGTGTC GGAGGACATG ACGGTTCATT TCACATCAAC TCTTATGGCC
    CTAATACGCA CGGCACTGGA TATCAAAATA GCTAAAGGAG GTGCTGACAG GCAACAGCTG
    GACTCAGAGC TGCAGAAAGA TATACTTGCC ATTTGGCCAA ATCTCTCCCA GAAAATGATC
    GACTTACTTG TGCCAATGCC AAGAGCCTCT GATCTGACGG TGGGGAAGAT TTACGCTGCA
    ATGATGATCA TGGATTACTA TAAACAGAGC AAAGCGAAAA AACAAAGGCA GCAGCTGGAG
    GAGCAGAAAA ATGCACCTAT GTTTCAGAGA ATGGAGGCTT CTGCCCTCCC TGAGGAGATC
    TTATCCAATG CCAAAGCTTT ACCATACCTT CAGCAAGACA CTCTATCAGG CCTGGGTGGA
    TTTCCTTCAC TGAGTCCCTT GTCCCCAGAA GAAGTATTCC AGCCGCCATG CCTAGACGGG
    GAAGAGGAGG AAGACTTTGA AGAGCAGCTG TCTCTGGAGC CTGAGGTTAG AGAGTTTAGA
    AGGGAGCAGC TCCCTAACCG AGGCAGCTAC ATCCCTGTGG AGATGCTGGA ACGTCCAGCG
    TCTGTGAGAG CTTCTTCCAT GCCACGTCTG GCTGAGAATC CTCAGGTCCT GTCAGACGTA
    AGCTCCATGC GTCGCTCCTT CTCCACAACT GCAGAGAAGC GAGTGCGCAG CTCGTGGCTA
    GATGAGTTCA CTATGGACAG GAGTAGCACT GACTGTGCAT ACAGATACCA GAGGCGCAGT
    TATCATGCAT CCTTAAGACT GTCTGCACAT CGCCTGAATA CAGATACAGG TCACTGGTCA
    GATAATCACC GTTCTGGCGG AAGGGAGCGA GGAAGGTCCA AAGAGAGAAA ACACTTGCTG
    TCACCCGATC CTTCCCGCTG TAATTCTGAG GAAAGGAGCC CACAAGCTGC CTGTGAACAC
    CGAGATCACC AACTGTCCCC TGGGCATACT CCCAGTAGTG TACAGATTCT TGACTCTCTG
    AGTGAAAGTT GTACCCCTTC AGACACCAGC ACCCCAAGAA GAGGCCGTAG ACAGCTTCCT
    TCTGTGCCTC CAACTCCTAG ACCCCTTATC TCTTTCTCCT CGTTAATCCG CCGGCAGAAA
    CACAGCCCCC CTCCTCAAAA TAAAAATGAT ACTATCCTTC CTAGTGAGCC TGGTCTGACT
    GAGTCTTCCT GCTCCCTTGG GGGTCATCAG AGCTCCCAGT CCACACCACA GCATTATATC
    TCAGAGCCCT ACCTGGCACT GCATGAGGAG ATGCAAAGCT CAGACTGCCC AGCAGAGGAC
    ACACTCACCT TTGAGGTTGC TGTGGCTACT AGTCTGGGCC GCTCGAACAC CATTGGCTCT
    GCCCCTCTAC GTCATGGCTG GCAAATGCCC AATGGTCACT ACCGGCGTCG AAGGAAGGGA
    GGAATTCCAC CAGTGATACC CTCCAGAGAG TTAAGTGACA CAGAAGAGGA TGATCGATGT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017949087.1
    CDS9..6431
    Translation

    Target ORF information:

    RefSeq Version XM_018093598.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calcium channel, voltage-dependent, R type, alpha 1E subunit (cacna1e), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018093598.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGATCCTTG CCACAATTAT TGCAAACTGC ATTGTCCTGG CTTTGGAGCA GCATCTTCCA 
    GGCGATGACA AGACTCCAAT GTCTCGCAGA TTGGAAAAGA CTGAGCCCTA TTTCATTGGG
    ATATTTTGCT TTGAAGCTGG AATTAAAATT GTCGCTCTGG GACTGGTTTT TCACAAAGGT
    TCTTATCTTC GGAATGGCTG GAATGTCATG GACTTTATAG TAGTGCTCAG TGGCATCCTG
    GCCACAGCTG GAACTCACTT CAATACTCAC GTGGACCTGC GTACTTTGCG GGCAGTGCGT
    GTTCTCAGGC CGTTGAAGCT GGTCTCGGGG ATTCCCAGTT TACAGATTGT GTTGAAGTCA
    ATCATGAAGG CGATGGTCCC TCTGCTACAG ATTGGATTGT TGCTCTTCTT TGCCATCCTT
    ATGTTTGCTA TCATCGGCCT TGAGTTTTAC AGTGGAAAGC TACACAAGGC CTGTTACAGG
    AACAACTCTG GAATGGAGCT AGACCCCATG GATCCACCTC ACCCTTGTGG AGTACCCCGC
    TGCCCAGCTG GGTATGAGTG CAAGGAATGG ATCGGACCCA ATGATGGAAT AACACAGTTT
    GACAACATCT TATTTGCGGT GCTGACAGTC TTCCAGTGTA TAACTATGGA GGGCTGGACC
    ACAGTGCTGT ACAATACCAA TGATGCCTTA GGAGCCACAT GGAACTGGCT GTACTTCATT
    CCCCTCATTA TCATCGGATC CTTCTTTGTC CTTAATCTTG TCCTTGGAGT GCTGTCCGGT
    GAATTTGCCA AAGAGAGGGA AAGAGTGGAA AACCGCCGGG CTTTCATGAA GTTGCGCAGG
    CAGCAGCAGA TTGAGAGGGA GTTGAATGGC TATCGGGCAT GGATAGATAA AGCTGAGGAA
    GTCATGTTGG CAGAAGAAAA CAAAAATTCT GGGACCTCTG CTTTAGAAGT TCTTAGGCGA
    GCAACCATTA AACGAAGTAG AACAGAAGCC ATGAACCGGG ACTCTACTGA GGACCATTGT
    GTTGACATAT CCTCTGTTGG CACCCCCCTT GCTAGAGCAA GCATCAAAAG TACCAAAATG
    GATGGGGCAT CATATTTTCG GCACAAGGAA AAACTATTGC GTGTCTCCGT TCGTCACATG
    ATCAAATCCC AGGTTTTCTA CTGGATGGTC CTGGGAGTGG TAGCACTTAA CACTTTCTGT
    GTGGCTATTG TCCATCACAA CCAGACCCTG TGGCTCTCAA ATGTTCTTTA CTATGCAGAA
    TTTCTGTTTC TTGGGTTATT TTTGGTGGAG ATGGCTTTTA AAATGTATGG AATGGGTCCA
    CGTCTTTACT TTCACTCATC CTTCAACTGC TTTGATTGCG GGGTCACAAT AGGTAGTATA
    TTTGAAGTAC TTTGGGCCAT ATTTAGACCA GGAACTTCCT TTGGAATCAG TGTCTTAAGA
    GCTCTCCGAC TTTTGAGAAT CTTCAAAATA ACAAAATACT GGGCCTCACT ACGAAACCTT
    GTAGTATCAT TGATGAATTC TATGAAATCC ATCATCAGCC TGCTCTTCCT TCTATTCCTC
    TTCATAGTTG TTTTTGCTCT CCTTGGAATG CAGTTATATG GTGGCAGATT CAATTTTAAT
    GATGGCACTC CTTCTGCAAA CTTTGATACA TTCCCTGCAG CCATCATGAC TGTATTCCAG
    ATTCTGACAG GAGAGGATTG GAATGAAGTG ATGTACAATG GTATTCGCTC CCAAGGTGGT
    GTTCGATCCG GAATGTGGTC TTCCATATAC TTTATTGTCC TTACCCTTTT TGGAAATTAC
    ACACTGCTAA ATGTATTTCT TGCCATTGCT GTGGACAACC TGGCTAATGC ACAGGAGTTA
    ACTAAGGATG AGCAAGAAGA AGAGGAAGCT TTCAATCAGA AGCATGCACT GCAAAAAGCG
    AAGGAGGTCA GCCCCATGTC TGCACCTAAT GCATCATGTA TTGAGAAGGA GCGCAGAAAA
    CGGCACACAT TGTCTATATG GGAGCAGCGC ACAAGCCAGC TAAGGAGGCA CATACAGATG
    TCTAGTCAAG AAGCTCTCCA GAGTGAAGAA CTGGCAGCAT TGAATCCACA CTCCAGTATC
    TATCGCAAGA CAAAGGAAGC TGCTGAACAA AACACAGAAA AAGAAGAAGT GCATCAAGAC
    GCAAAACTGG CAATAGACCC AGTTGAAGGA CCACCAGAAG GTGAAGGAAG CCCTTCTAAT
    AGAAATCTCA GGGAAAACTG GCGCCAGAAG TCCAGGCATG GAAATTCTGA AAACACTGAA
    CCAAATGGAA CAACTCCAGA AGAAGCAATG CGCCGCAGAC AAAGTCAGAG ACGCAGCCGT
    CATAGAAGAG TTAGAACTGA AGGTCGTGAG CAGTGTAGCA ATCCAAACAG TACCTCAGCA
    AGCAGAGAAG GTGGTCTTGA CAAAGTCTCT GCAGAGGATG GAAGAACAAT TCCAGAAGAG
    AAGAGCAATG GAGAAGCCAA AGAAGTCAAA GGGGTTGATG ATGAGCCTCT AAAGATTTGT
    ATATCTTCAA GAAATTGCGA GGACAGAAAT ATCAGGACTT TGGGTTGTGA GGGTATTGCC
    TTGAACGATA CACCCCTGCA GGATGTGAAC AGCCAAGAGA AGGACAGTAG CCCTCCTGAT
    TTGGATTGCA GTGAGCAGGC TCTGTTGGGC AGCATTCAGA GCCAGGAAAG CCCTAATCCA
    ACCAGCTGCA GTTTGCCAGA TGTGTCCAGC AGCACCGAGA GAGTCACAGA GAGCACCGTC
    ATTACCTTAC CAGATACTGG AATGCCCACC GACTGCACCG TGGTACAGAT GAGTGAAGGC
    AGGGATGGAG AAGTCAACTT TGGGTCTTCG CAGCAAGAAA AAGAAGAGGA GGAAGCTGAG
    GATGGAGAAG AAAGAACTAT AAAAAGAAAA GAGAAATTGT TGGAGTCCCC CAAAGCCATT
    GTACCGTACA GTTCAATGCT CATCTTCAGC TCCACCAATC CCATAAGAAA AGTATGTCAT
    TACATTGTTA ACCTACGATA CTTTGAGATG AGCATCCTGC TGGTAATAGC AGCAAGTAGT
    ATAGCCTTAG CAGCGGAGGA CCCTGTACTG ACCAACTCGG ACCGCAACAA AGTTCTTCGC
    TATTTTGACT ATGTTTTCAC AGGGGTCTTC ACTTTTGAGA TGATTATAAA GATGATTGAC
    CAAGGTTTGA TTTTACACGA GGGCTCCTAC TTTCGTGACC TATGGAACAT CTTGGATTTT
    GTTGTTGTTG TGGGAGCCTT AATTGCGTTT GCCTTAGCGA CTAACAAAGG CCGGGACATC
    AAAACCATTA AGTCTCTACG TGTGTTACGG GTGCTGCGTC CACTAAAAAC AATCAAACGC
    CTGCCAAAAC TGAAGGCAGT CTTTGATTGT GTGGTTAACT CTTTAAAGAA TGTCTTTAAT
    ATTCTCATCG TATACAAACT CTTCATGTTC ATATTTGCTG TAATCGCTGT CCAGCTGTTT
    AAGGGCAAGT TCTTCTATTG TACAGACAGC TCAAAGGAGA CAGAGAAAGA GTGCATAGGG
    AGTTACATCG ATCACGAAAA GAACAAGATG GAGGTGAAGC GGAGAGAGTG GAAAAGACAT
    GATTTCCATT ATGACAACAT AATATGGGCT CTTCTTACCC TCTTTACTGT ATCAACAGGG
    GAAGGCTGGC CTCAGGTTCT GCAGCACTCT GTTGATATAA CTGAAGAAGA TAGGGGTCCA
    AGCCGAAGCA ACCGTATGGA GATGTCCATT TTCTATGTTA TCTACTTTGT GGTCTTCCCC
    TTCTTCTTTG TAAATATATT TGTGGCTCTG ATTATCATCA CATTCCAGGA GCAAGGAGAC
    AAGATGATGG AGGAATGTTC CCTAGAAAAG AATGAGAGAG CCTGCATAGA CTTTGCAATA
    AGTGCTAAGC CACTTACACG ATATATGCCA CAGAACAGGC ACACATTCCA GTACCGGGTC
    TGGCACTTCG TGGTCTCCCC ATCATTTGAA TACACTATCA TGGCTATGAT TGCACTAAAC
    ACAGTGGTAC TCATGATGAA GTATTATTCT GCGCCATACA CATATGAGCT TGCATTAAAG
    TATCTCAATA TTGCATTCAC AATGCTATTT TCTCTGGAGT GCATCCTGAA AATAATTGCA
    TTTGGGTTTT TGAATTACTT TCGGGATACT TGGAACATTT TTGACTTCAT TACTGTAATT
    GGTAGCATCA CGGAAATTAT TCTAACAGAT AGTAAGTTGG TGAACACCAG CAGCTTTAAT
    ATGAGCTTTC TGAAGCTGTT CCGAGCGGCG CGACTTATTA AACTTCTCCG GCAAGGGTAC
    ACCATCCGGA TCCTGCTGTG GACTTTTGTT CAGTCCTTTA AGGCACTTCC TTATGTGTGT
    CTTCTCATTG CAATGTTGTT CTTCATCTAT GCCATTATTG GGATGCAGGT GTTTGGCAAT
    ATCCGCCTGG AAGAGGACAG TCATATTAAT AGGCACAACA ATTTTAGAAG CTTCTTTAGT
    TCTCTTATGT TACTCTTCAG GAGTGCCACA GGAGAAGCGT GGCAGGAGAT CATGCTTTCA
    TGCCTTAGTG ATAAGGGTTG TGAGCCAGAC ACCAGTGCAG CCTCTGGACA CAATGAGAAT
    GAGCGCTGTG GAACTGAACT TGCATATGTT TATTTTGTTT CATTCATTTT CTTCTGCTCC
    TTCCTCATGC TAAATCTGTT TGTGGCTGTC ATTATGGACA ACTTTGAGTA CTTGACACGG
    GACTCTTCCA TTTTGGGACC TCATCACTTG GATGAGTTTG TTCGGATATG GGCTGAATAT
    GATCGAGCAG CATGTGGCAG GATCCCCTAT AAGGATATGT ACAAATTAGT CCGAGTCATC
    TCGCCTCCGC TCGGCCTAGG AGAGAACTGC CCATACAGGG TGGCATGCAA GAGATTGGTC
    CTGATGAATA TGCCAGTGTC GGAGGACATG ACGGTTCATT TCACATCAAC TCTTATGGCC
    CTAATACGCA CGGCACTGGA TATCAAAATA GCTAAAGGAG GTGCTGACAG GCAACAGCTG
    GACTCAGAGC TGCAGAAAGA TATACTTGCC ATTTGGCCAA ATCTCTCCCA GAAAATGATC
    GACTTACTTG TGCCAATGCC AAGAGCCTCT GATCTGACGG TGGGGAAGAT TTACGCTGCA
    ATGATGATCA TGGATTACTA TAAACAGAGC AAAGCGAAAA AACAAAGGCA GCAGCTGGAG
    GAGCAGAAAA ATGCACCTAT GTTTCAGAGA ATGGAGGCTT CTGCCCTCCC TGAGGAGATC
    TTATCCAATG CCAAAGCTTT ACCATACCTT CAGCAAGACA CTCTATCAGG CCTGGGTGGA
    TTTCCTTCAC TGAGTCCCTT GTCCCCAGAA GAAGTATTCC AGCCGCCATG CCTAGACGGG
    GAAGAGGAGG AAGACTTTGA AGAGCAGCTG TCTCTGGAGC CTGAGGTTAG AGAGTTTAGA
    AGGGAGCAGC TCCCTAACCG AGGCAGCTAC ATCCCTGTGG AGATGCTGGA ACGTCCAGCG
    TCTGTGAGAG CTTCTTCCAT GCCACGTCTG GCTGAGAATC CTCAGGTCCT GTCAGACGTA
    AGCTCCATGC GTCGCTCCTT CTCCACAACT GCAGAGAAGC GAGTGCGCAG CTCGTGGCTA
    GATGAGTTCA CTATGGACAG GAGTAGCACT GACTGTGCAT ACAGATACCA GAGGCGCAGT
    TATCATGCAT CCTTAAGACT GTCTGCACAT CGCCTGAATA CAGATACAGG TCACTGGTCA
    GATAATCACC GTTCTGGCGG AAGGGAGCGA GGAAGGTCCA AAGAGAGAAA ACACTTGCTG
    TCACCCGATC CTTCCCGCTG TAATTCTGAG GAAAGGAGCC CACAAGCTGC CTGTGAACAC
    CGAGATCACC AACTGTCCCC TGGGCATACT CCCAGTAGTG TACAGATTCT TGACTCTCTG
    AGTGAAAGTT GTACCCCTTC AGACACCAGC ACCCCAAGAA GAGGCCGTAG ACAGCTTCCT
    TCTGTGCCTC CAACTCCTAG ACCCCTTATC TCTTTCTCCT CGTTAATCCG CCGGCAGAAA
    CACAGCCCCC CTCCTCAAAA TAAAAATGAT ACTATCCTTC CTAGTGAGCC TGGTCTGACT
    GAGTCTTCCT GCTCCCTTGG GGGTCATCAG AGCTCCCAGT CCACACCACA GCATTATATC
    TCAGAGCCCT ACCTGGCACT GCATGAGGAG ATGCAAAGCT CAGACTGCCC AGCAGAGGAC
    ACACTCACCT TTGAGGTTGC TGTGGCTACT AGTCTGGGCC GCTCGAACAC CATTGGCTCT
    GCCCCTCTAC GTCATGGCTG GCAAATGCCC AATGGTCACT ACCGGCGTCG AAGGAAGGGA
    GGAATTCCAC CAGTGATACC CTCCAGAGAG TTAAGTGACA CAGAAGAGGA TGATCGATGT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa57229
    Clone ID Related Accession (Same CDS sequence) XM_031900979.1
    Accession Version XM_031900979.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7182bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGGTATTAA AGATGCTGGG ATTAAGCTCC TGTTCAACCA GTGCTCATAC ACTCAATGAG 
    CCTGACCAGT CAAGCACACT AGCCATACCA GCATTGGATG CCGACACTCA GAGATATGTC
    TTGGCCCTTC CAGAAGCAGA GACATGGCGC AAGCATCGAT TGGGCCTGAC ACAGGCTACT
    CACTCTTGCA ATCTTCTAGA ACCTGAGACA CTTTCTGAAA CCCTAGTTTC ACGCACTGCA
    AGTTACGATG CCTTGTATGA CCTGCGAGCA AGAGAAGCGG AGTCTAACTT CCCATTTGAA
    GGCAGCAGCA TGTTTGAGGT GACGTCTGGG ATGGATTCTG AGTTTCCTTT AAGCCCCCAT
    GTTATAAAGC GAAGGAGAGG AGGCCTGATA GAACAAAAGG ATATCATCAA GGCCCACGAA
    GCTCATAAGA TTCAGAGTAC TCCCCAGGCT CGACGGAAGG AATGGGAGAT GGCTCGTTTC
    GGAGAGGCTG TGACTGGCAG GTTGAGCTCC GCAGATGGGG GCTCAGAGAG GAGCAAGACC
    CTACAAGGTT CCCCAGCTGC ACCTGTTGGG ACCCAAGCAG CTTTCAAGCA ATCCAAAGCC
    CAGAGGGCAA GGACCATGGC TTTATACAAC CCCATCCCTG TCCGACAGAA CTGCTTCACC
    GTGAACAGGT CTCTCTTCAT TTTCGGAGAA GATAATATTA TCAGGAAATA CGCCAAGAAG
    CTCATTGATT GGCCTCCTTT TGAATACATG ATCCTTGCCA CAATTATTGC AAACTGCATT
    GTCCTGGCTT TGGAGCAGCA TCTTCCAGGC GATGACAAGA CTCCAATGTC TCGCAGATTG
    GAAAAGACTG AGCCCTATTT CATTGGGATA TTTTGCTTTG AAGCTGGAAT TAAAATTGTC
    GCTCTGGGAC TGGTTTTTCA CAAAGGTTCT TATCTTCGGA ATGGCTGGAA TGTCATGGAC
    TTTATAGTAG TGCTCAGTGG CATCCTGGCC ACAGCTGGAA CTCACTTCAA TACTCACGTG
    GACCTGCGTA CTTTGCGGGC AGTGCGTGTT CTCAGGCCGT TGAAGCTGGT CTCGGGGATT
    CCCAGTTTAC AGATTGTGTT GAAGTCAATC ATGAAGGCGA TGGTCCCTCT GCTACAGATT
    GGATTGTTGC TCTTCTTTGC CATCCTTATG TTTGCTATCA TCGGCCTTGA GTTTTACAGT
    GGAAAGCTAC ACAAGGCCTG TTACAGGAAC AACTCTGGAA TGGAGCTAGA CCCCATGGAT
    CCACCTCACC CTTGTGGAGT GCCCCGCTGC CCAGCTGGGT ATGAGTGCAA GGAATGGATC
    GGACCCAATG ATGGAATAAC ACAGTTTGAC AACATCCTAT TTGCGGTGCT GACAGTCTTC
    CAGTGTATAA CTATGGAGGG CTGGACCACA GTGCTGTACA ATACCAATGA TGCCTTAGGA
    GCCACATGGA ACTGGCTGTA CTTCATTCCC CTCATTATCA TCGGATCCTT CTTTGTCCTT
    AATCTTGTCC TTGGAGTGCT GTCCGGTGAA TTTGCCAAAG AGAGGGAAAG AGTGGAAAAC
    CGCCGGGCTT TCATGAAGTT GCGCAGGCAG CAGCAGATTG AGAGGGAGTT GAATGGCTAT
    CGGGCATGGA TAGATAAAGC TGAGGAAGTC ATGTTGGCAG AAGAAAACAA AAATTCTGGG
    ACCTCTGCTT TAGAAGTTCT TAGGCGAGCA ACCATTAAAC GAAGTAGAAC AGAAGCCATG
    AACCGGGACT CTACTGAGGA CCATTGTGTT GACATATCCT CTGTTGGCAC CCCCCTTGCT
    AGAGCAAGCA TCAAAAGTAC CAAAATGGAT GGGGCATCAT ATTTTCGGCA CAAGGAAAAA
    CTATTGCGTG TCTCCGTTCG TCACATGATC AAATCCCAGG TTTTCTACTG GATGGTCCTG
    GGAGTGGTAG CACTTAACAC TTTCTGTGTG GCTATTGTCC ATCACAACCA GACCCTGTGG
    CTCTCAAATG TTCTTTACTA TGCAGAATTT CTGTTTCTTG GGTTATTTTT GGTGGAGATG
    GCTTTTAAAA TGTATGGAAT GGGTCCACGT CTTTACTTTC ACTCATCCTT CAACTGCTTT
    GATTGCGGGG TCACAATAGG TAGTATATTT GAAGTACTTT GGGCCATATT TAGACCAGGA
    ACTTCCTTTG GAATCAGTGT CTTAAGAGCT CTCCGACTTT TGAGAATCTT CAAAATAACA
    AAATACTGGG CCTCACTACG AAACCTTGTA GTATCATTGA TGAATTCTAT GAAATCCATC
    ATCAGCCTGC TCTTCCTTCT ATTCCTCTTC ATAGTTGTTT TTGCTCTCCT TGGAATGCAG
    TTATATGGTG GCAGATTCAA TTTTAATGAT GGCACTCCTT CTGCAAACTT TGATACATTC
    CCTGCAGCCA TCATGACTGT ATTCCAGATT CTGACAGGAG AGGATTGGAA TGAAGTGATG
    TACAATGGTA TTCGCTCCCA AGGTGGTGTT CGATCCGGAA TGTGGTCTTC CATATACTTT
    ATTGTCCTTA CCCTTTTTGG AAATTACACA CTGCTAAATG TATTTCTTGC CATTGCTGTG
    GACAACCTGG CTAATGCACA GGAGTTAACT AAGGATGAGC AAGAAGAAGA GGAAGCTTTC
    AATCAGAAGC ATGCACTGCA AAAAGCGAAG GAGGTCAGCC CCATGTCTGC ACCTAATGCA
    TCATGTATTG AGAAGGAGCG CAGAAAACGG CACACATTGT CTATATGGGA GCAGCGCACA
    AGCCAGCTAA GGAGGCACAT ACAGATGTCT AGTCAAGAAG CTCTCCAGAG TGAAGAACTG
    GCAGCATTGA ATCCACACTC CAGTATCTAT CGCAAGACAA AGGAAGCTGC TGAACAAAAC
    ACAGAAAAAG AAGAAGTGCA TCAAGACGCA AAACTGGCAA TAGACCCAGT TGAAGGACCA
    CCAGAAGGTG AAGGAAGCCC TTCTAATAGA AATCTCAGGG AAAACTGGCG CCAGAAGTCC
    AGGCATGGAA ATTCTGAAAA CACTGAGCCA AATGGAACAA CTCCAGAAGA AGCAATGCGC
    CGCAGACAAA GTCAGAGACG CAGCCGTCAT AGAAGAGTTA GAACTGAAGG TCGTGAGCAG
    TGTAGCAATC CAAACAGTAC CTCAGCAAGC AGAGAAGGTG GTCTTGACAA AGTCTCTGCA
    GAGGATGGAA GAACAATTCC AGAAGAGAAG AGCAATGGAG AAGCCAAAGA AGTCAAAGGG
    GTTGATGATG AGCCTCTAAA GATTTGTATA TCTTCAAGAA ATTGCGAGGA CAGAAATATC
    AGGACTTTGG GTTGTGAGGG TATTGCCTTG AACGATACAC CCCTGCAGGA TGTGAACAGC
    CAAGAGAAGG ACAGTAGCCC TCCTGATTTG GATTGCAGTG AGCAGGCTCT GTTGGGCAGC
    ATTCAGAGCC AGGAAAGCCC TAATCCAACC AGCTGCAGTT TGCCAGATGT GTCCAGCAGC
    ACCGAGAGAG TCACAGAGAG CACCGTCATT ACCTTACCAG ATACTGGAAT GCCCACCGAC
    TGCACCGTGG TACAGATGAG TGAAGGCAGG GATGGAGAAG TCAACTTTGG GTCTTCGCAG
    CAAGAAAAAG AAGAGGAGGA AGCTGAGGAT GGAGAAGAAA GAACTATAAA AAGAAAAGAG
    AAATTGTTGG AGTCCCCCAA AGCCATTGTA CCGTACAGTT CAATGCTCAT CTTCAGCTCC
    ACCAATCCCA TAAGAAAAGT ATGTCATTAC ATTGTTAACC TACGATACTT TGAGATGAGC
    ATCCTGCTGG TAATAGCAGC AAGTAGTATA GCCTTAGCAG CGGAGGACCC TGTACTGACC
    AACTCGGACC GCAACAAAGT TCTTCGCTAT TTTGACTATG TTTTCACAGG GGTCTTCACT
    TTTGAGATGA TTATAAAGAT GATTGACCAA GGTTTGATTT TACACGAGGG CTCCTACTTT
    CGTGACCTAT GGAACATCTT GGATTTTGTT GTTGTTGTGG GAGCCTTAAT TGCGTTTGCC
    TTAGCGAATG TTTTGGGGAC TAACAAAGGC CGGGACATCA AAACCATTAA GTCTCTACGT
    GTGTTACGGG TGCTGCGTCC ACTAAAAACA ATCAAACGCC TGCCAAAACT GAAGGCAGTC
    TTTGATTGTG TGGTTAACTC TTTAAAGAAT GTCTTTAATA TTCTCATCGT ATACAAACTC
    TTCATGTTCA TATTTGCTGT AATCGCTGTC CAGCTGTTTA AGGGCAAGTT CTTCTATTGT
    ACAGACAGCT CAAAGGAGAC AGAGAAAGAG TGCATAGGGA GTTACATCGA TCACGAAAAG
    AACAAGATGG AGGTGAAGCG GAGAGAGTGG AAAAGACATG ATTTCCATTA TGACAACATA
    ATATGGGCTC TTCTTACCCT CTTTACTGTA TCAACAGGGG AAGGCTGGCC TCAGGTTCTG
    CAGCACTCTG TTGATATAAC TGAAGAAGAT AGGGGTCCAA GCCGAAGCAA CCGTATGGAG
    ATGTCCATTT TCTATGTTAT CTACTTTGTG GTCTTCCCCT TCTTCTTTGT AAATATATTT
    GTGGCTCTGA TTATCATCAC ATTCCAGGAG CAAGGAGACA AGATGATGGA GGAATGTTCC
    CTAGAAAAGA ATGAGAGAGC CTGCATAGAC TTTGCAATAA GTGCTAAGCC ACTTACACGA
    TATATGCCAC AGAACAGGCA CACATTCCAG TACCGGGTCT GGCACTTCGT GGTCTCCCCA
    TCATTTGAAT ACACTATCAT GGCTATGATT GCACTAAACA CAGTGGTACT CATGATGAAG
    TATTATTCTG CGCCATACAC ATATGAGCTT GCATTAAAGT ATCTCAATAT TGCATTCACA
    ATGCTATTTT CTCTGGAGTG CATCCTGAAA ATAATTGCAT TTGGGTTTTT GAATTACTTT
    CGGGATACTT GGAACATTTT TGACTTCATT ACTGTAATTG GTAGCATCAC GGAAATTATT
    CTAACAGATA GTAAGTTGGT GAACACCAGC AGCTTTAATA TGAGCTTTCT GAAGCTGTTC
    CGAGCGGCGC GACTTATTAA ACTTCTCCGG CAAGGGTACA CCATCCGGAT CCTGCTGTGG
    ACTTTTGTTC AGTCCTTTAA GGCACTTCCT TATGTGTGTC TTCTCATTGC AATGTTGTTC
    TTCATCTATG CCATTATTGG GATGCAGGTG TTTGGCAATA TCCGCCTGGA AGAGGACAGT
    CATATTAATA GGCACAACAA TTTTAGAAGC TTCTTTAGTT CTCTTATGTT ACTCTTCAGG
    AGTGCCACAG GAGAAGCGTG GCAGGAGATC ATGCTTTCAT GCCTTAGTGA TAAGGGTTGT
    GAGCCAGACA CCAGTGCAGC CTCTGGACAC AACGAGAATG AGCGCTGTGG AACTGAACTT
    GCATATGTTT ATTTTGTTTC ATTCATTTTC TTCTGCTCCT TCCTCATGCT AAATCTGTTT
    GTGGCTGTCA TTATGGACAA CTTTGAGTAC TTGACACGGG ACTCTTCCAT TTTGGGACCT
    CATCACTTGG ATGAGTTTGT TCGGATATGG GCTGAATATG ATCGAGCAGC ATGTGGCCGT
    ATCCATTACA CAGAGATGTA TGACATGCTG ACTCTCATGT CACCACCTCT AGGCCTTGGA
    AAGAGATGCC CTAACAAAGT GGCTTATAAG AGATTGGTCC TGATGAATAT GCCAGTGTCG
    GAGGACATGA CGGTTCATTT CACATCAACT CTTATGGCCC TAATACGCAC GGCACTGGAT
    ATCAAAATAG CTAAAGGAGG TGCTGACAGG CAACAGCTGG ACTCAGAGCT GCAGAAAGAT
    ATACTTGCCA TTTGGCCAAA TCTCTCCCAG AAAATGATCG ACTTACTTGT GCCAATGCCA
    AGAGCCTCTG ATCTGACGGT GGGGAAGATT TACGCTGCAA TGATGATCAT GGATTACTAT
    AAACAGAGCA AAGCGAAAAA ACAAAGGCAG CAGCTGGAGG AGCAGAAAAA TGCACCTATG
    TTTCAGAGAA TGGAGGCTTC TGCCCTCCCT GAGGAGATCT TATCCAATGC CAAAGCTTTA
    CCATACCTTC AGCAAGACAC TCTATCAGGC CTGGGTGGAT TTCCTTCACT GAGTCCCTTG
    TCCCCAGAAG AAGTATTCCA GCCGCCATGC CTAGACGGGG AAGAGGAGGA AGACTTTGAA
    GAGCAGCTGT CTCTGGAGCC TGAGGTTAGA GAGTTTAGAA GGGAGCAGCT CCCTAACCGA
    GGCAGCTACA TCCCTGTGGA GATGCTGGAA CGTCCAGCGT CTGTGAGAGC TTCTTCCATG
    CCACGTCTGG CTGAGAATCC TCAGGTCCTG TCAGACGTAA GCTCCATGCG TCGCTCCTTC
    TCCACAACTG CAGAGAAGCG AGTGCGCAGC TCGTGGCTAG ATGAGTTCAC TATGGACAGG
    AGTAGCACTG ACTGTGCATA CAGATACCAG AGGCGCAGTT ATCATGCATC CTTAAGACTG
    TCTGCACATC GCCTGAATAC AGATACAGGT CACTGGTCAG ATAATCACCG TTCTGGCGGA
    AGGGAGCGAG GAAGGTCCAA AGAGAGAAAA CACTTGCTGT CACCCGATCC TTCCCGCTGT
    AATTCTGAGG AAAGGAGCCC ACAAGCTGCC TGTGAACACC GAGATCACCA ACTGTCCCCT
    GGGCATACTC CCAGTAGTGT ACAGATTCTT GACTCTCTGA GTGAAAGTTG TACCCCTTCA
    GACACCAGCA CCCCAAGAAG AGGCCGTAGA CAGCTTCCTT CTGTGCCTCC AACTCCTAGA
    CCCCTTATCT CTTTCTCCTC GTTAATCCGC CGGCAGAAAC ACAGCCCCCC TCCTCAAAAT
    AAAAATGATA CTATCCTTCC TAGTGAGCCT GGTCTGACTG AGTCTTCCTG CTCCCTTGGG
    GGTCATCAGA GCTCCCAGTC CACACCACAG CATTATATCT CAGAGCCCTA CCTGGCACTG
    CATGAGGAGA TGCAAAGCTC AGACTGCCCA GCAGAGGACA CACTCACCTT TGAGGTTGCT
    GTGGCTACTA GTCTGGGCCG CTCGAACACC ATTGGCTCTG CCCCTCTACG TCATGGCTGG
    CAAATGCCCA ATGGTCACTA CCGGCGTCGA AGGAAGGGAG GAATTCCACC AGTGATACCC
    TCCAGAGAGT TAAGTGACAC AGAAGAGGAT GATCGATGTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031756839.1
    CDS284..7465
    Translation

    Target ORF information:

    RefSeq Version XM_031900979.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calcium channel, voltage-dependent, R type, alpha 1E subunit (cacna1e), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031900979.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGGTATTAA AGATGCTGGG ATTAAGCTCC TGTTCAACCA GTGCTCATAC ACTCAATGAG 
    CCTGACCAGT CAAGCACACT AGCCATACCA GCATTGGATG CCGACACTCA GAGATATGTC
    TTGGCCCTTC CAGAAGCAGA GACATGGCGC AAGCATCGAT TGGGCCTGAC ACAGGCTACT
    CACTCTTGCA ATCTTCTAGA ACCTGAGACA CTTTCTGAAA CCCTAGTTTC ACGCACTGCA
    AGTTACGATG CCTTGTATGA CCTGCGAGCA AGAGAAGCGG AGTCTAACTT CCCATTTGAA
    GGCAGCAGCA TGTTTGAGGT GACGTCTGGG ATGGATTCTG AGTTTCCTTT AAGCCCCCAT
    GTTATAAAGC GAAGGAGAGG AGGCCTGATA GAACAAAAGG ATATCATCAA GGCCCACGAA
    GCTCATAAGA TTCAGAGTAC TCCCCAGGCT CGACGGAAGG AATGGGAGAT GGCTCGTTTC
    GGAGAGGCTG TGACTGGCAG GTTGAGCTCC GCAGATGGGG GCTCAGAGAG GAGCAAGACC
    CTACAAGGTT CCCCAGCTGC ACCTGTTGGG ACCCAAGCAG CTTTCAAGCA ATCCAAAGCC
    CAGAGGGCAA GGACCATGGC TTTATACAAC CCCATCCCTG TCCGACAGAA CTGCTTCACC
    GTGAACAGGT CTCTCTTCAT TTTCGGAGAA GATAATATTA TCAGGAAATA CGCCAAGAAG
    CTCATTGATT GGCCTCCTTT TGAATACATG ATCCTTGCCA CAATTATTGC AAACTGCATT
    GTCCTGGCTT TGGAGCAGCA TCTTCCAGGC GATGACAAGA CTCCAATGTC TCGCAGATTG
    GAAAAGACTG AGCCCTATTT CATTGGGATA TTTTGCTTTG AAGCTGGAAT TAAAATTGTC
    GCTCTGGGAC TGGTTTTTCA CAAAGGTTCT TATCTTCGGA ATGGCTGGAA TGTCATGGAC
    TTTATAGTAG TGCTCAGTGG CATCCTGGCC ACAGCTGGAA CTCACTTCAA TACTCACGTG
    GACCTGCGTA CTTTGCGGGC AGTGCGTGTT CTCAGGCCGT TGAAGCTGGT CTCGGGGATT
    CCCAGTTTAC AGATTGTGTT GAAGTCAATC ATGAAGGCGA TGGTCCCTCT GCTACAGATT
    GGATTGTTGC TCTTCTTTGC CATCCTTATG TTTGCTATCA TCGGCCTTGA GTTTTACAGT
    GGAAAGCTAC ACAAGGCCTG TTACAGGAAC AACTCTGGAA TGGAGCTAGA CCCCATGGAT
    CCACCTCACC CTTGTGGAGT GCCCCGCTGC CCAGCTGGGT ATGAGTGCAA GGAATGGATC
    GGACCCAATG ATGGAATAAC ACAGTTTGAC AACATCCTAT TTGCGGTGCT GACAGTCTTC
    CAGTGTATAA CTATGGAGGG CTGGACCACA GTGCTGTACA ATACCAATGA TGCCTTAGGA
    GCCACATGGA ACTGGCTGTA CTTCATTCCC CTCATTATCA TCGGATCCTT CTTTGTCCTT
    AATCTTGTCC TTGGAGTGCT GTCCGGTGAA TTTGCCAAAG AGAGGGAAAG AGTGGAAAAC
    CGCCGGGCTT TCATGAAGTT GCGCAGGCAG CAGCAGATTG AGAGGGAGTT GAATGGCTAT
    CGGGCATGGA TAGATAAAGC TGAGGAAGTC ATGTTGGCAG AAGAAAACAA AAATTCTGGG
    ACCTCTGCTT TAGAAGTTCT TAGGCGAGCA ACCATTAAAC GAAGTAGAAC AGAAGCCATG
    AACCGGGACT CTACTGAGGA CCATTGTGTT GACATATCCT CTGTTGGCAC CCCCCTTGCT
    AGAGCAAGCA TCAAAAGTAC CAAAATGGAT GGGGCATCAT ATTTTCGGCA CAAGGAAAAA
    CTATTGCGTG TCTCCGTTCG TCACATGATC AAATCCCAGG TTTTCTACTG GATGGTCCTG
    GGAGTGGTAG CACTTAACAC TTTCTGTGTG GCTATTGTCC ATCACAACCA GACCCTGTGG
    CTCTCAAATG TTCTTTACTA TGCAGAATTT CTGTTTCTTG GGTTATTTTT GGTGGAGATG
    GCTTTTAAAA TGTATGGAAT GGGTCCACGT CTTTACTTTC ACTCATCCTT CAACTGCTTT
    GATTGCGGGG TCACAATAGG TAGTATATTT GAAGTACTTT GGGCCATATT TAGACCAGGA
    ACTTCCTTTG GAATCAGTGT CTTAAGAGCT CTCCGACTTT TGAGAATCTT CAAAATAACA
    AAATACTGGG CCTCACTACG AAACCTTGTA GTATCATTGA TGAATTCTAT GAAATCCATC
    ATCAGCCTGC TCTTCCTTCT ATTCCTCTTC ATAGTTGTTT TTGCTCTCCT TGGAATGCAG
    TTATATGGTG GCAGATTCAA TTTTAATGAT GGCACTCCTT CTGCAAACTT TGATACATTC
    CCTGCAGCCA TCATGACTGT ATTCCAGATT CTGACAGGAG AGGATTGGAA TGAAGTGATG
    TACAATGGTA TTCGCTCCCA AGGTGGTGTT CGATCCGGAA TGTGGTCTTC CATATACTTT
    ATTGTCCTTA CCCTTTTTGG AAATTACACA CTGCTAAATG TATTTCTTGC CATTGCTGTG
    GACAACCTGG CTAATGCACA GGAGTTAACT AAGGATGAGC AAGAAGAAGA GGAAGCTTTC
    AATCAGAAGC ATGCACTGCA AAAAGCGAAG GAGGTCAGCC CCATGTCTGC ACCTAATGCA
    TCATGTATTG AGAAGGAGCG CAGAAAACGG CACACATTGT CTATATGGGA GCAGCGCACA
    AGCCAGCTAA GGAGGCACAT ACAGATGTCT AGTCAAGAAG CTCTCCAGAG TGAAGAACTG
    GCAGCATTGA ATCCACACTC CAGTATCTAT CGCAAGACAA AGGAAGCTGC TGAACAAAAC
    ACAGAAAAAG AAGAAGTGCA TCAAGACGCA AAACTGGCAA TAGACCCAGT TGAAGGACCA
    CCAGAAGGTG AAGGAAGCCC TTCTAATAGA AATCTCAGGG AAAACTGGCG CCAGAAGTCC
    AGGCATGGAA ATTCTGAAAA CACTGAGCCA AATGGAACAA CTCCAGAAGA AGCAATGCGC
    CGCAGACAAA GTCAGAGACG CAGCCGTCAT AGAAGAGTTA GAACTGAAGG TCGTGAGCAG
    TGTAGCAATC CAAACAGTAC CTCAGCAAGC AGAGAAGGTG GTCTTGACAA AGTCTCTGCA
    GAGGATGGAA GAACAATTCC AGAAGAGAAG AGCAATGGAG AAGCCAAAGA AGTCAAAGGG
    GTTGATGATG AGCCTCTAAA GATTTGTATA TCTTCAAGAA ATTGCGAGGA CAGAAATATC
    AGGACTTTGG GTTGTGAGGG TATTGCCTTG AACGATACAC CCCTGCAGGA TGTGAACAGC
    CAAGAGAAGG ACAGTAGCCC TCCTGATTTG GATTGCAGTG AGCAGGCTCT GTTGGGCAGC
    ATTCAGAGCC AGGAAAGCCC TAATCCAACC AGCTGCAGTT TGCCAGATGT GTCCAGCAGC
    ACCGAGAGAG TCACAGAGAG CACCGTCATT ACCTTACCAG ATACTGGAAT GCCCACCGAC
    TGCACCGTGG TACAGATGAG TGAAGGCAGG GATGGAGAAG TCAACTTTGG GTCTTCGCAG
    CAAGAAAAAG AAGAGGAGGA AGCTGAGGAT GGAGAAGAAA GAACTATAAA AAGAAAAGAG
    AAATTGTTGG AGTCCCCCAA AGCCATTGTA CCGTACAGTT CAATGCTCAT CTTCAGCTCC
    ACCAATCCCA TAAGAAAAGT ATGTCATTAC ATTGTTAACC TACGATACTT TGAGATGAGC
    ATCCTGCTGG TAATAGCAGC AAGTAGTATA GCCTTAGCAG CGGAGGACCC TGTACTGACC
    AACTCGGACC GCAACAAAGT TCTTCGCTAT TTTGACTATG TTTTCACAGG GGTCTTCACT
    TTTGAGATGA TTATAAAGAT GATTGACCAA GGTTTGATTT TACACGAGGG CTCCTACTTT
    CGTGACCTAT GGAACATCTT GGATTTTGTT GTTGTTGTGG GAGCCTTAAT TGCGTTTGCC
    TTAGCGAATG TTTTGGGGAC TAACAAAGGC CGGGACATCA AAACCATTAA GTCTCTACGT
    GTGTTACGGG TGCTGCGTCC ACTAAAAACA ATCAAACGCC TGCCAAAACT GAAGGCAGTC
    TTTGATTGTG TGGTTAACTC TTTAAAGAAT GTCTTTAATA TTCTCATCGT ATACAAACTC
    TTCATGTTCA TATTTGCTGT AATCGCTGTC CAGCTGTTTA AGGGCAAGTT CTTCTATTGT
    ACAGACAGCT CAAAGGAGAC AGAGAAAGAG TGCATAGGGA GTTACATCGA TCACGAAAAG
    AACAAGATGG AGGTGAAGCG GAGAGAGTGG AAAAGACATG ATTTCCATTA TGACAACATA
    ATATGGGCTC TTCTTACCCT CTTTACTGTA TCAACAGGGG AAGGCTGGCC TCAGGTTCTG
    CAGCACTCTG TTGATATAAC TGAAGAAGAT AGGGGTCCAA GCCGAAGCAA CCGTATGGAG
    ATGTCCATTT TCTATGTTAT CTACTTTGTG GTCTTCCCCT TCTTCTTTGT AAATATATTT
    GTGGCTCTGA TTATCATCAC ATTCCAGGAG CAAGGAGACA AGATGATGGA GGAATGTTCC
    CTAGAAAAGA ATGAGAGAGC CTGCATAGAC TTTGCAATAA GTGCTAAGCC ACTTACACGA
    TATATGCCAC AGAACAGGCA CACATTCCAG TACCGGGTCT GGCACTTCGT GGTCTCCCCA
    TCATTTGAAT ACACTATCAT GGCTATGATT GCACTAAACA CAGTGGTACT CATGATGAAG
    TATTATTCTG CGCCATACAC ATATGAGCTT GCATTAAAGT ATCTCAATAT TGCATTCACA
    ATGCTATTTT CTCTGGAGTG CATCCTGAAA ATAATTGCAT TTGGGTTTTT GAATTACTTT
    CGGGATACTT GGAACATTTT TGACTTCATT ACTGTAATTG GTAGCATCAC GGAAATTATT
    CTAACAGATA GTAAGTTGGT GAACACCAGC AGCTTTAATA TGAGCTTTCT GAAGCTGTTC
    CGAGCGGCGC GACTTATTAA ACTTCTCCGG CAAGGGTACA CCATCCGGAT CCTGCTGTGG
    ACTTTTGTTC AGTCCTTTAA GGCACTTCCT TATGTGTGTC TTCTCATTGC AATGTTGTTC
    TTCATCTATG CCATTATTGG GATGCAGGTG TTTGGCAATA TCCGCCTGGA AGAGGACAGT
    CATATTAATA GGCACAACAA TTTTAGAAGC TTCTTTAGTT CTCTTATGTT ACTCTTCAGG
    AGTGCCACAG GAGAAGCGTG GCAGGAGATC ATGCTTTCAT GCCTTAGTGA TAAGGGTTGT
    GAGCCAGACA CCAGTGCAGC CTCTGGACAC AACGAGAATG AGCGCTGTGG AACTGAACTT
    GCATATGTTT ATTTTGTTTC ATTCATTTTC TTCTGCTCCT TCCTCATGCT AAATCTGTTT
    GTGGCTGTCA TTATGGACAA CTTTGAGTAC TTGACACGGG ACTCTTCCAT TTTGGGACCT
    CATCACTTGG ATGAGTTTGT TCGGATATGG GCTGAATATG ATCGAGCAGC ATGTGGCCGT
    ATCCATTACA CAGAGATGTA TGACATGCTG ACTCTCATGT CACCACCTCT AGGCCTTGGA
    AAGAGATGCC CTAACAAAGT GGCTTATAAG AGATTGGTCC TGATGAATAT GCCAGTGTCG
    GAGGACATGA CGGTTCATTT CACATCAACT CTTATGGCCC TAATACGCAC GGCACTGGAT
    ATCAAAATAG CTAAAGGAGG TGCTGACAGG CAACAGCTGG ACTCAGAGCT GCAGAAAGAT
    ATACTTGCCA TTTGGCCAAA TCTCTCCCAG AAAATGATCG ACTTACTTGT GCCAATGCCA
    AGAGCCTCTG ATCTGACGGT GGGGAAGATT TACGCTGCAA TGATGATCAT GGATTACTAT
    AAACAGAGCA AAGCGAAAAA ACAAAGGCAG CAGCTGGAGG AGCAGAAAAA TGCACCTATG
    TTTCAGAGAA TGGAGGCTTC TGCCCTCCCT GAGGAGATCT TATCCAATGC CAAAGCTTTA
    CCATACCTTC AGCAAGACAC TCTATCAGGC CTGGGTGGAT TTCCTTCACT GAGTCCCTTG
    TCCCCAGAAG AAGTATTCCA GCCGCCATGC CTAGACGGGG AAGAGGAGGA AGACTTTGAA
    GAGCAGCTGT CTCTGGAGCC TGAGGTTAGA GAGTTTAGAA GGGAGCAGCT CCCTAACCGA
    GGCAGCTACA TCCCTGTGGA GATGCTGGAA CGTCCAGCGT CTGTGAGAGC TTCTTCCATG
    CCACGTCTGG CTGAGAATCC TCAGGTCCTG TCAGACGTAA GCTCCATGCG TCGCTCCTTC
    TCCACAACTG CAGAGAAGCG AGTGCGCAGC TCGTGGCTAG ATGAGTTCAC TATGGACAGG
    AGTAGCACTG ACTGTGCATA CAGATACCAG AGGCGCAGTT ATCATGCATC CTTAAGACTG
    TCTGCACATC GCCTGAATAC AGATACAGGT CACTGGTCAG ATAATCACCG TTCTGGCGGA
    AGGGAGCGAG GAAGGTCCAA AGAGAGAAAA CACTTGCTGT CACCCGATCC TTCCCGCTGT
    AATTCTGAGG AAAGGAGCCC ACAAGCTGCC TGTGAACACC GAGATCACCA ACTGTCCCCT
    GGGCATACTC CCAGTAGTGT ACAGATTCTT GACTCTCTGA GTGAAAGTTG TACCCCTTCA
    GACACCAGCA CCCCAAGAAG AGGCCGTAGA CAGCTTCCTT CTGTGCCTCC AACTCCTAGA
    CCCCTTATCT CTTTCTCCTC GTTAATCCGC CGGCAGAAAC ACAGCCCCCC TCCTCAAAAT
    AAAAATGATA CTATCCTTCC TAGTGAGCCT GGTCTGACTG AGTCTTCCTG CTCCCTTGGG
    GGTCATCAGA GCTCCCAGTC CACACCACAG CATTATATCT CAGAGCCCTA CCTGGCACTG
    CATGAGGAGA TGCAAAGCTC AGACTGCCCA GCAGAGGACA CACTCACCTT TGAGGTTGCT
    GTGGCTACTA GTCTGGGCCG CTCGAACACC ATTGGCTCTG CCCCTCTACG TCATGGCTGG
    CAAATGCCCA ATGGTCACTA CCGGCGTCGA AGGAAGGGAG GAATTCCACC AGTGATACCC
    TCCAGAGAGT TAAGTGACAC AGAAGAGGAT GATCGATGTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa57230
    Clone ID Related Accession (Same CDS sequence) XM_018093597.2
    Accession Version XM_018093597.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7182bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018093597.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGGTATTAA AGATGCTGGG ATTAAGCTCC TGTTCAACCA GTGCTCATAC ACTCAATGAG 
    CCTGACCAGT CAAGCACACT AGCCATACCA GCATTGGATG CCGACACTCA GAGATATGTC
    TTGGCCCTTC CAGAAGCAGA GACATGGCGC AAGCATCGAT TGGGCCTGAC ACAGGCTACT
    CACTCTTGCA ATCTTCTAGA ACCTGAGACA CTTTCTGAAA CCCTAGTTTC ACGCACTGCA
    AGTTACGATG CCTTGTATGA CCTGCGAGCA AGAGAAGCGG AGTCTAACTT CCCATTTGAA
    GGCAGCAGCA TGTTTGAGGT GACGTCTGGG ATGGATTCTG AGTTTCCTTT AAGCCCCCAT
    GTTATAAAGC GAAGGAGAGG AGGCCTGATA GAACAAAAGG ATATCATCAA GGCCCACGAA
    GCTCATAAGA TTCAGAGTAC TCCCCAGGCT CGACGGAAGG AATGGGAGAT GGCTCGTTTC
    GGAGAGGCTG TGACTGGCAG GTTGAGCTCC GCAGATGGGG GCTCAGAGAG GAGCAAGACC
    CTACAAGGTT CCCCAGCTGC ACCTGTTGGG ACCCAAGCAG CTTTCAAGCA ATCCAAAGCC
    CAGAGGGCAA GGACCATGGC TTTATACAAC CCCATCCCTG TCCGACAGAA CTGCTTCACC
    GTGAACAGGT CTCTCTTCAT TTTCGGAGAA GATAATATTA TCAGGAAATA CGCCAAGAAG
    CTCATTGATT GGCCTCCTTT TGAATACATG ATCCTTGCCA CAATTATTGC AAACTGCATT
    GTCCTGGCTT TGGAGCAGCA TCTTCCAGGC GATGACAAGA CTCCAATGTC TCGCAGATTG
    GAAAAGACTG AGCCCTATTT CATTGGGATA TTTTGCTTTG AAGCTGGAAT TAAAATTGTC
    GCTCTGGGAC TGGTTTTTCA CAAAGGTTCT TATCTTCGGA ATGGCTGGAA TGTCATGGAC
    TTTATAGTAG TGCTCAGTGG CATCCTGGCC ACAGCTGGAA CTCACTTCAA TACTCACGTG
    GACCTGCGTA CTTTGCGGGC AGTGCGTGTT CTCAGGCCGT TGAAGCTGGT CTCGGGGATT
    CCCAGTTTAC AGATTGTGTT GAAGTCAATC ATGAAGGCGA TGGTCCCTCT GCTACAGATT
    GGATTGTTGC TCTTCTTTGC CATCCTTATG TTTGCTATCA TCGGCCTTGA GTTTTACAGT
    GGAAAGCTAC ACAAGGCCTG TTACAGGAAC AACTCTGGAA TGGAGCTAGA CCCCATGGAT
    CCACCTCACC CTTGTGGAGT GCCCCGCTGC CCAGCTGGGT ATGAGTGCAA GGAATGGATC
    GGACCCAATG ATGGAATAAC ACAGTTTGAC AACATCCTAT TTGCGGTGCT GACAGTCTTC
    CAGTGTATAA CTATGGAGGG CTGGACCACA GTGCTGTACA ATACCAATGA TGCCTTAGGA
    GCCACATGGA ACTGGCTGTA CTTCATTCCC CTCATTATCA TCGGATCCTT CTTTGTCCTT
    AATCTTGTCC TTGGAGTGCT GTCCGGTGAA TTTGCCAAAG AGAGGGAAAG AGTGGAAAAC
    CGCCGGGCTT TCATGAAGTT GCGCAGGCAG CAGCAGATTG AGAGGGAGTT GAATGGCTAT
    CGGGCATGGA TAGATAAAGC TGAGGAAGTC ATGTTGGCAG AAGAAAACAA AAATTCTGGG
    ACCTCTGCTT TAGAAGTTCT TAGGCGAGCA ACCATTAAAC GAAGTAGAAC AGAAGCCATG
    AACCGGGACT CTACTGAGGA CCATTGTGTT GACATATCCT CTGTTGGCAC CCCCCTTGCT
    AGAGCAAGCA TCAAAAGTAC CAAAATGGAT GGGGCATCAT ATTTTCGGCA CAAGGAAAAA
    CTATTGCGTG TCTCCGTTCG TCACATGATC AAATCCCAGG TTTTCTACTG GATGGTCCTG
    GGAGTGGTAG CACTTAACAC TTTCTGTGTG GCTATTGTCC ATCACAACCA GACCCTGTGG
    CTCTCAAATG TTCTTTACTA TGCAGAATTT CTGTTTCTTG GGTTATTTTT GGTGGAGATG
    GCTTTTAAAA TGTATGGAAT GGGTCCACGT CTTTACTTTC ACTCATCCTT CAACTGCTTT
    GATTGCGGGG TCACAATAGG TAGTATATTT GAAGTACTTT GGGCCATATT TAGACCAGGA
    ACTTCCTTTG GAATCAGTGT CTTAAGAGCT CTCCGACTTT TGAGAATCTT CAAAATAACA
    AAATACTGGG CCTCACTACG AAACCTTGTA GTATCATTGA TGAATTCTAT GAAATCCATC
    ATCAGCCTGC TCTTCCTTCT ATTCCTCTTC ATAGTTGTTT TTGCTCTCCT TGGAATGCAG
    TTATATGGTG GCAGATTCAA TTTTAATGAT GGCACTCCTT CTGCAAACTT TGATACATTC
    CCTGCAGCCA TCATGACTGT ATTCCAGATT CTGACAGGAG AGGATTGGAA TGAAGTGATG
    TACAATGGTA TTCGCTCCCA AGGTGGTGTT CGATCCGGAA TGTGGTCTTC CATATACTTT
    ATTGTCCTTA CCCTTTTTGG AAATTACACA CTGCTAAATG TATTTCTTGC CATTGCTGTG
    GACAACCTGG CTAATGCACA GGAGTTAACT AAGGATGAGC AAGAAGAAGA GGAAGCTTTC
    AATCAGAAGC ATGCACTGCA AAAAGCGAAG GAGGTCAGCC CCATGTCTGC ACCTAATGCA
    TCATGTATTG AGAAGGAGCG CAGAAAACGG CACACATTGT CTATATGGGA GCAGCGCACA
    AGCCAGCTAA GGAGGCACAT ACAGATGTCT AGTCAAGAAG CTCTCCAGAG TGAAGAACTG
    GCAGCATTGA ATCCACACTC CAGTATCTAT CGCAAGACAA AGGAAGCTGC TGAACAAAAC
    ACAGAAAAAG AAGAAGTGCA TCAAGACGCA AAACTGGCAA TAGACCCAGT TGAAGGACCA
    CCAGAAGGTG AAGGAAGCCC TTCTAATAGA AATCTCAGGG AAAACTGGCG CCAGAAGTCC
    AGGCATGGAA ATTCTGAAAA CACTGAGCCA AATGGAACAA CTCCAGAAGA AGCAATGCGC
    CGCAGACAAA GTCAGAGACG CAGCCGTCAT AGAAGAGTTA GAACTGAAGG TCGTGAGCAG
    TGTAGCAATC CAAACAGTAC CTCAGCAAGC AGAGAAGGTG GTCTTGACAA AGTCTCTGCA
    GAGGATGGAA GAACAATTCC AGAAGAGAAG AGCAATGGAG AAGCCAAAGA AGTCAAAGGG
    GTTGATGATG AGCCTCTAAA GATTTGTATA TCTTCAAGAA ATTGCGAGGA CAGAAATATC
    AGGACTTTGG GTTGTGAGGG TATTGCCTTG AACGATACAC CCCTGCAGGA TGTGAACAGC
    CAAGAGAAGG ACAGTAGCCC TCCTGATTTG GATTGCAGTG AGCAGGCTCT GTTGGGCAGC
    ATTCAGAGCC AGGAAAGCCC TAATCCAACC AGCTGCAGTT TGCCAGATGT GTCCAGCAGC
    ACCGAGAGAG TCACAGAGAG CACCGTCATT ACCTTACCAG ATACTGGAAT GCCCACCGAC
    TGCACCGTGG TACAGATGAG TGAAGGCAGG GATGGAGAAG TCAACTTTGG GTCTTCGCAG
    CAAGAAAAAG AAGAGGAGGA AGCTGAGGAT GGAGAAGAAA GAACTATAAA AAGAAAAGAG
    AAATTGTTGG AGTCCCCCAA AGCCATTGTA CCGTACAGTT CAATGCTCAT CTTCAGCTCC
    ACCAATCCCA TAAGAAAAGT ATGTCATTAC ATTGTTAACC TACGATACTT TGAGATGAGC
    ATCCTGCTGG TAATAGCAGC AAGTAGTATA GCCTTAGCAG CGGAGGACCC TGTACTGACC
    AACTCGGACC GCAACAAAGT TCTTCGCTAT TTTGACTATG TTTTCACAGG GGTCTTCACT
    TTTGAGATGA TTATAAAGAT GATTGACCAA GGTTTGATTT TACACGAGGG CTCCTACTTT
    CGTGACCTAT GGAACATCTT GGATTTTGTT GTTGTTGTGG GAGCCTTAAT TGCGTTTGCC
    TTAGCGAATG TTTTGGGGAC TAACAAAGGC CGGGACATCA AAACCATTAA GTCTCTACGT
    GTGTTACGGG TGCTGCGTCC ACTAAAAACA ATCAAACGCC TGCCAAAACT GAAGGCAGTC
    TTTGATTGTG TGGTTAACTC TTTAAAGAAT GTCTTTAATA TTCTCATCGT ATACAAACTC
    TTCATGTTCA TATTTGCTGT AATCGCTGTC CAGCTGTTTA AGGGCAAGTT CTTCTATTGT
    ACAGACAGCT CAAAGGAGAC AGAGAAAGAG TGCATAGGGA GTTACATCGA TCACGAAAAG
    AACAAGATGG AGGTGAAGCG GAGAGAGTGG AAAAGACATG ATTTCCATTA TGACAACATA
    ATATGGGCTC TTCTTACCCT CTTTACTGTA TCAACAGGGG AAGGCTGGCC TCAGGTTCTG
    CAGCACTCTG TTGATATAAC TGAAGAAGAT AGGGGTCCAA GCCGAAGCAA CCGTATGGAG
    ATGTCCATTT TCTATGTTAT CTACTTTGTG GTCTTCCCCT TCTTCTTTGT AAATATATTT
    GTGGCTCTGA TTATCATCAC ATTCCAGGAG CAAGGAGACA AGATGATGGA GGAATGTTCC
    CTAGAAAAGA ATGAGAGAGC CTGCATAGAC TTTGCAATAA GTGCTAAGCC ACTTACACGA
    TATATGCCAC AGAACAGGCA CACATTCCAG TACCGGGTCT GGCACTTCGT GGTCTCCCCA
    TCATTTGAAT ACACTATCAT GGCTATGATT GCACTAAACA CAGTGGTACT CATGATGAAG
    TATTATTCTG CGCCATACAC ATATGAGCTT GCATTAAAGT ATCTCAATAT TGCATTCACA
    ATGCTATTTT CTCTGGAGTG CATCCTGAAA ATAATTGCAT TTGGGTTTTT GAATTACTTT
    CGGGATACTT GGAACATTTT TGACTTCATT ACTGTAATTG GTAGCATCAC GGAAATTATT
    CTAACAGATA GTAAGTTGGT GAACACCAGC AGCTTTAATA TGAGCTTTCT GAAGCTGTTC
    CGAGCGGCGC GACTTATTAA ACTTCTCCGG CAAGGGTACA CCATCCGGAT CCTGCTGTGG
    ACTTTTGTTC AGTCCTTTAA GGCACTTCCT TATGTGTGTC TTCTCATTGC AATGTTGTTC
    TTCATCTATG CCATTATTGG GATGCAGGTG TTTGGCAATA TCCGCCTGGA AGAGGACAGT
    CATATTAATA GGCACAACAA TTTTAGAAGC TTCTTTAGTT CTCTTATGTT ACTCTTCAGG
    AGTGCCACAG GAGAAGCGTG GCAGGAGATC ATGCTTTCAT GCCTTAGTGA TAAGGGTTGT
    GAGCCAGACA CCAGTGCAGC CTCTGGACAC AACGAGAATG AGCGCTGTGG AACTGAACTT
    GCATATGTTT ATTTTGTTTC ATTCATTTTC TTCTGCTCCT TCCTCATGCT AAATCTGTTT
    GTGGCTGTCA TTATGGACAA CTTTGAGTAC TTGACACGGG ACTCTTCCAT TTTGGGACCT
    CATCACTTGG ATGAGTTTGT TCGGATATGG GCTGAATATG ATCGAGCAGC ATGTGGCAGG
    ATCCCCTATA AGGATATGTA CAAATTAGTC CGAGTCATCT CGCCTCCGCT CGGCCTAGGA
    GAGAACTGCC CATACAGGGT GGCATGCAAG AGATTGGTCC TGATGAATAT GCCAGTGTCG
    GAGGACATGA CGGTTCATTT CACATCAACT CTTATGGCCC TAATACGCAC GGCACTGGAT
    ATCAAAATAG CTAAAGGAGG TGCTGACAGG CAACAGCTGG ACTCAGAGCT GCAGAAAGAT
    ATACTTGCCA TTTGGCCAAA TCTCTCCCAG AAAATGATCG ACTTACTTGT GCCAATGCCA
    AGAGCCTCTG ATCTGACGGT GGGGAAGATT TACGCTGCAA TGATGATCAT GGATTACTAT
    AAACAGAGCA AAGCGAAAAA ACAAAGGCAG CAGCTGGAGG AGCAGAAAAA TGCACCTATG
    TTTCAGAGAA TGGAGGCTTC TGCCCTCCCT GAGGAGATCT TATCCAATGC CAAAGCTTTA
    CCATACCTTC AGCAAGACAC TCTATCAGGC CTGGGTGGAT TTCCTTCACT GAGTCCCTTG
    TCCCCAGAAG AAGTATTCCA GCCGCCATGC CTAGACGGGG AAGAGGAGGA AGACTTTGAA
    GAGCAGCTGT CTCTGGAGCC TGAGGTTAGA GAGTTTAGAA GGGAGCAGCT CCCTAACCGA
    GGCAGCTACA TCCCTGTGGA GATGCTGGAA CGTCCAGCGT CTGTGAGAGC TTCTTCCATG
    CCACGTCTGG CTGAGAATCC TCAGGTCCTG TCAGACGTAA GCTCCATGCG TCGCTCCTTC
    TCCACAACTG CAGAGAAGCG AGTGCGCAGC TCGTGGCTAG ATGAGTTCAC TATGGACAGG
    AGTAGCACTG ACTGTGCATA CAGATACCAG AGGCGCAGTT ATCATGCATC CTTAAGACTG
    TCTGCACATC GCCTGAATAC AGATACAGGT CACTGGTCAG ATAATCACCG TTCTGGCGGA
    AGGGAGCGAG GAAGGTCCAA AGAGAGAAAA CACTTGCTGT CACCCGATCC TTCCCGCTGT
    AATTCTGAGG AAAGGAGCCC ACAAGCTGCC TGTGAACACC GAGATCACCA ACTGTCCCCT
    GGGCATACTC CCAGTAGTGT ACAGATTCTT GACTCTCTGA GTGAAAGTTG TACCCCTTCA
    GACACCAGCA CCCCAAGAAG AGGCCGTAGA CAGCTTCCTT CTGTGCCTCC AACTCCTAGA
    CCCCTTATCT CTTTCTCCTC GTTAATCCGC CGGCAGAAAC ACAGCCCCCC TCCTCAAAAT
    AAAAATGATA CTATCCTTCC TAGTGAGCCT GGTCTGACTG AGTCTTCCTG CTCCCTTGGG
    GGTCATCAGA GCTCCCAGTC CACACCACAG CATTATATCT CAGAGCCCTA CCTGGCACTG
    CATGAGGAGA TGCAAAGCTC AGACTGCCCA GCAGAGGACA CACTCACCTT TGAGGTTGCT
    GTGGCTACTA GTCTGGGCCG CTCGAACACC ATTGGCTCTG CCCCTCTACG TCATGGCTGG
    CAAATGCCCA ATGGTCACTA CCGGCGTCGA AGGAAGGGAG GAATTCCACC AGTGATACCC
    TCCAGAGAGT TAAGTGACAC AGAAGAGGAT GATCGATGTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017949086.2
    CDS285..7466
    Translation

    Target ORF information:

    RefSeq Version XM_018093597.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calcium channel, voltage-dependent, R type, alpha 1E subunit (cacna1e), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018093597.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGGTATTAA AGATGCTGGG ATTAAGCTCC TGTTCAACCA GTGCTCATAC ACTCAATGAG 
    CCTGACCAGT CAAGCACACT AGCCATACCA GCATTGGATG CCGACACTCA GAGATATGTC
    TTGGCCCTTC CAGAAGCAGA GACATGGCGC AAGCATCGAT TGGGCCTGAC ACAGGCTACT
    CACTCTTGCA ATCTTCTAGA ACCTGAGACA CTTTCTGAAA CCCTAGTTTC ACGCACTGCA
    AGTTACGATG CCTTGTATGA CCTGCGAGCA AGAGAAGCGG AGTCTAACTT CCCATTTGAA
    GGCAGCAGCA TGTTTGAGGT GACGTCTGGG ATGGATTCTG AGTTTCCTTT AAGCCCCCAT
    GTTATAAAGC GAAGGAGAGG AGGCCTGATA GAACAAAAGG ATATCATCAA GGCCCACGAA
    GCTCATAAGA TTCAGAGTAC TCCCCAGGCT CGACGGAAGG AATGGGAGAT GGCTCGTTTC
    GGAGAGGCTG TGACTGGCAG GTTGAGCTCC GCAGATGGGG GCTCAGAGAG GAGCAAGACC
    CTACAAGGTT CCCCAGCTGC ACCTGTTGGG ACCCAAGCAG CTTTCAAGCA ATCCAAAGCC
    CAGAGGGCAA GGACCATGGC TTTATACAAC CCCATCCCTG TCCGACAGAA CTGCTTCACC
    GTGAACAGGT CTCTCTTCAT TTTCGGAGAA GATAATATTA TCAGGAAATA CGCCAAGAAG
    CTCATTGATT GGCCTCCTTT TGAATACATG ATCCTTGCCA CAATTATTGC AAACTGCATT
    GTCCTGGCTT TGGAGCAGCA TCTTCCAGGC GATGACAAGA CTCCAATGTC TCGCAGATTG
    GAAAAGACTG AGCCCTATTT CATTGGGATA TTTTGCTTTG AAGCTGGAAT TAAAATTGTC
    GCTCTGGGAC TGGTTTTTCA CAAAGGTTCT TATCTTCGGA ATGGCTGGAA TGTCATGGAC
    TTTATAGTAG TGCTCAGTGG CATCCTGGCC ACAGCTGGAA CTCACTTCAA TACTCACGTG
    GACCTGCGTA CTTTGCGGGC AGTGCGTGTT CTCAGGCCGT TGAAGCTGGT CTCGGGGATT
    CCCAGTTTAC AGATTGTGTT GAAGTCAATC ATGAAGGCGA TGGTCCCTCT GCTACAGATT
    GGATTGTTGC TCTTCTTTGC CATCCTTATG TTTGCTATCA TCGGCCTTGA GTTTTACAGT
    GGAAAGCTAC ACAAGGCCTG TTACAGGAAC AACTCTGGAA TGGAGCTAGA CCCCATGGAT
    CCACCTCACC CTTGTGGAGT GCCCCGCTGC CCAGCTGGGT ATGAGTGCAA GGAATGGATC
    GGACCCAATG ATGGAATAAC ACAGTTTGAC AACATCCTAT TTGCGGTGCT GACAGTCTTC
    CAGTGTATAA CTATGGAGGG CTGGACCACA GTGCTGTACA ATACCAATGA TGCCTTAGGA
    GCCACATGGA ACTGGCTGTA CTTCATTCCC CTCATTATCA TCGGATCCTT CTTTGTCCTT
    AATCTTGTCC TTGGAGTGCT GTCCGGTGAA TTTGCCAAAG AGAGGGAAAG AGTGGAAAAC
    CGCCGGGCTT TCATGAAGTT GCGCAGGCAG CAGCAGATTG AGAGGGAGTT GAATGGCTAT
    CGGGCATGGA TAGATAAAGC TGAGGAAGTC ATGTTGGCAG AAGAAAACAA AAATTCTGGG
    ACCTCTGCTT TAGAAGTTCT TAGGCGAGCA ACCATTAAAC GAAGTAGAAC AGAAGCCATG
    AACCGGGACT CTACTGAGGA CCATTGTGTT GACATATCCT CTGTTGGCAC CCCCCTTGCT
    AGAGCAAGCA TCAAAAGTAC CAAAATGGAT GGGGCATCAT ATTTTCGGCA CAAGGAAAAA
    CTATTGCGTG TCTCCGTTCG TCACATGATC AAATCCCAGG TTTTCTACTG GATGGTCCTG
    GGAGTGGTAG CACTTAACAC TTTCTGTGTG GCTATTGTCC ATCACAACCA GACCCTGTGG
    CTCTCAAATG TTCTTTACTA TGCAGAATTT CTGTTTCTTG GGTTATTTTT GGTGGAGATG
    GCTTTTAAAA TGTATGGAAT GGGTCCACGT CTTTACTTTC ACTCATCCTT CAACTGCTTT
    GATTGCGGGG TCACAATAGG TAGTATATTT GAAGTACTTT GGGCCATATT TAGACCAGGA
    ACTTCCTTTG GAATCAGTGT CTTAAGAGCT CTCCGACTTT TGAGAATCTT CAAAATAACA
    AAATACTGGG CCTCACTACG AAACCTTGTA GTATCATTGA TGAATTCTAT GAAATCCATC
    ATCAGCCTGC TCTTCCTTCT ATTCCTCTTC ATAGTTGTTT TTGCTCTCCT TGGAATGCAG
    TTATATGGTG GCAGATTCAA TTTTAATGAT GGCACTCCTT CTGCAAACTT TGATACATTC
    CCTGCAGCCA TCATGACTGT ATTCCAGATT CTGACAGGAG AGGATTGGAA TGAAGTGATG
    TACAATGGTA TTCGCTCCCA AGGTGGTGTT CGATCCGGAA TGTGGTCTTC CATATACTTT
    ATTGTCCTTA CCCTTTTTGG AAATTACACA CTGCTAAATG TATTTCTTGC CATTGCTGTG
    GACAACCTGG CTAATGCACA GGAGTTAACT AAGGATGAGC AAGAAGAAGA GGAAGCTTTC
    AATCAGAAGC ATGCACTGCA AAAAGCGAAG GAGGTCAGCC CCATGTCTGC ACCTAATGCA
    TCATGTATTG AGAAGGAGCG CAGAAAACGG CACACATTGT CTATATGGGA GCAGCGCACA
    AGCCAGCTAA GGAGGCACAT ACAGATGTCT AGTCAAGAAG CTCTCCAGAG TGAAGAACTG
    GCAGCATTGA ATCCACACTC CAGTATCTAT CGCAAGACAA AGGAAGCTGC TGAACAAAAC
    ACAGAAAAAG AAGAAGTGCA TCAAGACGCA AAACTGGCAA TAGACCCAGT TGAAGGACCA
    CCAGAAGGTG AAGGAAGCCC TTCTAATAGA AATCTCAGGG AAAACTGGCG CCAGAAGTCC
    AGGCATGGAA ATTCTGAAAA CACTGAGCCA AATGGAACAA CTCCAGAAGA AGCAATGCGC
    CGCAGACAAA GTCAGAGACG CAGCCGTCAT AGAAGAGTTA GAACTGAAGG TCGTGAGCAG
    TGTAGCAATC CAAACAGTAC CTCAGCAAGC AGAGAAGGTG GTCTTGACAA AGTCTCTGCA
    GAGGATGGAA GAACAATTCC AGAAGAGAAG AGCAATGGAG AAGCCAAAGA AGTCAAAGGG
    GTTGATGATG AGCCTCTAAA GATTTGTATA TCTTCAAGAA ATTGCGAGGA CAGAAATATC
    AGGACTTTGG GTTGTGAGGG TATTGCCTTG AACGATACAC CCCTGCAGGA TGTGAACAGC
    CAAGAGAAGG ACAGTAGCCC TCCTGATTTG GATTGCAGTG AGCAGGCTCT GTTGGGCAGC
    ATTCAGAGCC AGGAAAGCCC TAATCCAACC AGCTGCAGTT TGCCAGATGT GTCCAGCAGC
    ACCGAGAGAG TCACAGAGAG CACCGTCATT ACCTTACCAG ATACTGGAAT GCCCACCGAC
    TGCACCGTGG TACAGATGAG TGAAGGCAGG GATGGAGAAG TCAACTTTGG GTCTTCGCAG
    CAAGAAAAAG AAGAGGAGGA AGCTGAGGAT GGAGAAGAAA GAACTATAAA AAGAAAAGAG
    AAATTGTTGG AGTCCCCCAA AGCCATTGTA CCGTACAGTT CAATGCTCAT CTTCAGCTCC
    ACCAATCCCA TAAGAAAAGT ATGTCATTAC ATTGTTAACC TACGATACTT TGAGATGAGC
    ATCCTGCTGG TAATAGCAGC AAGTAGTATA GCCTTAGCAG CGGAGGACCC TGTACTGACC
    AACTCGGACC GCAACAAAGT TCTTCGCTAT TTTGACTATG TTTTCACAGG GGTCTTCACT
    TTTGAGATGA TTATAAAGAT GATTGACCAA GGTTTGATTT TACACGAGGG CTCCTACTTT
    CGTGACCTAT GGAACATCTT GGATTTTGTT GTTGTTGTGG GAGCCTTAAT TGCGTTTGCC
    TTAGCGAATG TTTTGGGGAC TAACAAAGGC CGGGACATCA AAACCATTAA GTCTCTACGT
    GTGTTACGGG TGCTGCGTCC ACTAAAAACA ATCAAACGCC TGCCAAAACT GAAGGCAGTC
    TTTGATTGTG TGGTTAACTC TTTAAAGAAT GTCTTTAATA TTCTCATCGT ATACAAACTC
    TTCATGTTCA TATTTGCTGT AATCGCTGTC CAGCTGTTTA AGGGCAAGTT CTTCTATTGT
    ACAGACAGCT CAAAGGAGAC AGAGAAAGAG TGCATAGGGA GTTACATCGA TCACGAAAAG
    AACAAGATGG AGGTGAAGCG GAGAGAGTGG AAAAGACATG ATTTCCATTA TGACAACATA
    ATATGGGCTC TTCTTACCCT CTTTACTGTA TCAACAGGGG AAGGCTGGCC TCAGGTTCTG
    CAGCACTCTG TTGATATAAC TGAAGAAGAT AGGGGTCCAA GCCGAAGCAA CCGTATGGAG
    ATGTCCATTT TCTATGTTAT CTACTTTGTG GTCTTCCCCT TCTTCTTTGT AAATATATTT
    GTGGCTCTGA TTATCATCAC ATTCCAGGAG CAAGGAGACA AGATGATGGA GGAATGTTCC
    CTAGAAAAGA ATGAGAGAGC CTGCATAGAC TTTGCAATAA GTGCTAAGCC ACTTACACGA
    TATATGCCAC AGAACAGGCA CACATTCCAG TACCGGGTCT GGCACTTCGT GGTCTCCCCA
    TCATTTGAAT ACACTATCAT GGCTATGATT GCACTAAACA CAGTGGTACT CATGATGAAG
    TATTATTCTG CGCCATACAC ATATGAGCTT GCATTAAAGT ATCTCAATAT TGCATTCACA
    ATGCTATTTT CTCTGGAGTG CATCCTGAAA ATAATTGCAT TTGGGTTTTT GAATTACTTT
    CGGGATACTT GGAACATTTT TGACTTCATT ACTGTAATTG GTAGCATCAC GGAAATTATT
    CTAACAGATA GTAAGTTGGT GAACACCAGC AGCTTTAATA TGAGCTTTCT GAAGCTGTTC
    CGAGCGGCGC GACTTATTAA ACTTCTCCGG CAAGGGTACA CCATCCGGAT CCTGCTGTGG
    ACTTTTGTTC AGTCCTTTAA GGCACTTCCT TATGTGTGTC TTCTCATTGC AATGTTGTTC
    TTCATCTATG CCATTATTGG GATGCAGGTG TTTGGCAATA TCCGCCTGGA AGAGGACAGT
    CATATTAATA GGCACAACAA TTTTAGAAGC TTCTTTAGTT CTCTTATGTT ACTCTTCAGG
    AGTGCCACAG GAGAAGCGTG GCAGGAGATC ATGCTTTCAT GCCTTAGTGA TAAGGGTTGT
    GAGCCAGACA CCAGTGCAGC CTCTGGACAC AACGAGAATG AGCGCTGTGG AACTGAACTT
    GCATATGTTT ATTTTGTTTC ATTCATTTTC TTCTGCTCCT TCCTCATGCT AAATCTGTTT
    GTGGCTGTCA TTATGGACAA CTTTGAGTAC TTGACACGGG ACTCTTCCAT TTTGGGACCT
    CATCACTTGG ATGAGTTTGT TCGGATATGG GCTGAATATG ATCGAGCAGC ATGTGGCAGG
    ATCCCCTATA AGGATATGTA CAAATTAGTC CGAGTCATCT CGCCTCCGCT CGGCCTAGGA
    GAGAACTGCC CATACAGGGT GGCATGCAAG AGATTGGTCC TGATGAATAT GCCAGTGTCG
    GAGGACATGA CGGTTCATTT CACATCAACT CTTATGGCCC TAATACGCAC GGCACTGGAT
    ATCAAAATAG CTAAAGGAGG TGCTGACAGG CAACAGCTGG ACTCAGAGCT GCAGAAAGAT
    ATACTTGCCA TTTGGCCAAA TCTCTCCCAG AAAATGATCG ACTTACTTGT GCCAATGCCA
    AGAGCCTCTG ATCTGACGGT GGGGAAGATT TACGCTGCAA TGATGATCAT GGATTACTAT
    AAACAGAGCA AAGCGAAAAA ACAAAGGCAG CAGCTGGAGG AGCAGAAAAA TGCACCTATG
    TTTCAGAGAA TGGAGGCTTC TGCCCTCCCT GAGGAGATCT TATCCAATGC CAAAGCTTTA
    CCATACCTTC AGCAAGACAC TCTATCAGGC CTGGGTGGAT TTCCTTCACT GAGTCCCTTG
    TCCCCAGAAG AAGTATTCCA GCCGCCATGC CTAGACGGGG AAGAGGAGGA AGACTTTGAA
    GAGCAGCTGT CTCTGGAGCC TGAGGTTAGA GAGTTTAGAA GGGAGCAGCT CCCTAACCGA
    GGCAGCTACA TCCCTGTGGA GATGCTGGAA CGTCCAGCGT CTGTGAGAGC TTCTTCCATG
    CCACGTCTGG CTGAGAATCC TCAGGTCCTG TCAGACGTAA GCTCCATGCG TCGCTCCTTC
    TCCACAACTG CAGAGAAGCG AGTGCGCAGC TCGTGGCTAG ATGAGTTCAC TATGGACAGG
    AGTAGCACTG ACTGTGCATA CAGATACCAG AGGCGCAGTT ATCATGCATC CTTAAGACTG
    TCTGCACATC GCCTGAATAC AGATACAGGT CACTGGTCAG ATAATCACCG TTCTGGCGGA
    AGGGAGCGAG GAAGGTCCAA AGAGAGAAAA CACTTGCTGT CACCCGATCC TTCCCGCTGT
    AATTCTGAGG AAAGGAGCCC ACAAGCTGCC TGTGAACACC GAGATCACCA ACTGTCCCCT
    GGGCATACTC CCAGTAGTGT ACAGATTCTT GACTCTCTGA GTGAAAGTTG TACCCCTTCA
    GACACCAGCA CCCCAAGAAG AGGCCGTAGA CAGCTTCCTT CTGTGCCTCC AACTCCTAGA
    CCCCTTATCT CTTTCTCCTC GTTAATCCGC CGGCAGAAAC ACAGCCCCCC TCCTCAAAAT
    AAAAATGATA CTATCCTTCC TAGTGAGCCT GGTCTGACTG AGTCTTCCTG CTCCCTTGGG
    GGTCATCAGA GCTCCCAGTC CACACCACAG CATTATATCT CAGAGCCCTA CCTGGCACTG
    CATGAGGAGA TGCAAAGCTC AGACTGCCCA GCAGAGGACA CACTCACCTT TGAGGTTGCT
    GTGGCTACTA GTCTGGGCCG CTCGAACACC ATTGGCTCTG CCCCTCTACG TCATGGCTGG
    CAAATGCCCA ATGGTCACTA CCGGCGTCGA AGGAAGGGAG GAATTCCACC AGTGATACCC
    TCCAGAGAGT TAAGTGACAC AGAAGAGGAT GATCGATGTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa57231
    Clone ID Related Accession (Same CDS sequence) XM_031900980.1
    Accession Version XM_031900980.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7170bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGGTATTAA AGATGCTGGG ATTAAGCTCC TGTTCAACCA GTGCTCATAC ACTCAATGAG 
    CCTGACCAGT CAAGCACACT AGCCATACCA GCATTGGATG CCGACACTCA GAGATATGTC
    TTGGCCCTTC CAGAAGCAGA GACATGGCGC AAGCATCGAT TGGGCCTGAC ACAGGCTACT
    CACTCTTGCA ATCTTCTAGA ACCTGAGACA CTTTCTGAAA CCCTAGTTTC ACGCACTGCA
    AGTTACGATG CCTTGTATGA CCTGCGAGCA AGAGAAGCGG AGTCTAACTT CCCATTTGAA
    GGCAGCAGCA TGTTTGAGGT GACGTCTGGG ATGGATTCTG AGTTTCCTTT AAGCCCCCAT
    GTTATAAAGC GAAGGAGAGG AGGCCTGATA GAACAAAAGG ATATCATCAA GGCCCACGAA
    GCTCATAAGA TTCAGAGTAC TCCCCAGGCT CGACGGAAGG AATGGGAGAT GGCTCGTTTC
    GGAGAGGCTG TGACTGGCAG GTTGAGCTCC GCAGATGGGG GCTCAGAGAG GAGCAAGACC
    CTACAAGGTT CCCCAGCTGC ACCTGTTGGG ACCCAAGCAG CTTTCAAGCA ATCCAAAGCC
    CAGAGGGCAA GGACCATGGC TTTATACAAC CCCATCCCTG TCCGACAGAA CTGCTTCACC
    GTGAACAGGT CTCTCTTCAT TTTCGGAGAA GATAATATTA TCAGGAAATA CGCCAAGAAG
    CTCATTGATT GGCCTCCTTT TGAATACATG ATCCTTGCCA CAATTATTGC AAACTGCATT
    GTCCTGGCTT TGGAGCAGCA TCTTCCAGGC GATGACAAGA CTCCAATGTC TCGCAGATTG
    GAAAAGACTG AGCCCTATTT CATTGGGATA TTTTGCTTTG AAGCTGGAAT TAAAATTGTC
    GCTCTGGGAC TGGTTTTTCA CAAAGGTTCT TATCTTCGGA ATGGCTGGAA TGTCATGGAC
    TTTATAGTAG TGCTCAGTGG CATCCTGGCC ACAGCTGGAA CTCACTTCAA TACTCACGTG
    GACCTGCGTA CTTTGCGGGC AGTGCGTGTT CTCAGGCCGT TGAAGCTGGT CTCGGGGATT
    CCCAGTTTAC AGATTGTGTT GAAGTCAATC ATGAAGGCGA TGGTCCCTCT GCTACAGATT
    GGATTGTTGC TCTTCTTTGC CATCCTTATG TTTGCTATCA TCGGCCTTGA GTTTTACAGT
    GGAAAGCTAC ACAAGGCCTG TTACAGGAAC AACTCTGGAA TGGAGCTAGA CCCCATGGAT
    CCACCTCACC CTTGTGGAGT GCCCCGCTGC CCAGCTGGGT ATGAGTGCAA GGAATGGATC
    GGACCCAATG ATGGAATAAC ACAGTTTGAC AACATCCTAT TTGCGGTGCT GACAGTCTTC
    CAGTGTATAA CTATGGAGGG CTGGACCACA GTGCTGTACA ATACCAATGA TGCCTTAGGA
    GCCACATGGA ACTGGCTGTA CTTCATTCCC CTCATTATCA TCGGATCCTT CTTTGTCCTT
    AATCTTGTCC TTGGAGTGCT GTCCGGTGAA TTTGCCAAAG AGAGGGAAAG AGTGGAAAAC
    CGCCGGGCTT TCATGAAGTT GCGCAGGCAG CAGCAGATTG AGAGGGAGTT GAATGGCTAT
    CGGGCATGGA TAGATAAAGC TGAGGAAGTC ATGTTGGCAG AAGAAAACAA AAATTCTGGG
    ACCTCTGCTT TAGAAGTTCT TAGGCGAGCA ACCATTAAAC GAAGTAGAAC AGAAGCCATG
    AACCGGGACT CTACTGAGGA CCATTGTGTT GACATATCCT CTGTTGGCAC CCCCCTTGCT
    AGAGCAAGCA TCAAAAGTAC CAAAATGGAT GGGGCATCAT ATTTTCGGCA CAAGGAAAAA
    CTATTGCGTG TCTCCGTTCG TCACATGATC AAATCCCAGG TTTTCTACTG GATGGTCCTG
    GGAGTGGTAG CACTTAACAC TTTCTGTGTG GCTATTGTCC ATCACAACCA GACCCTGTGG
    CTCTCAAATG TTCTTTACTA TGCAGAATTT CTGTTTCTTG GGTTATTTTT GGTGGAGATG
    GCTTTTAAAA TGTATGGAAT GGGTCCACGT CTTTACTTTC ACTCATCCTT CAACTGCTTT
    GATTGCGGGG TCACAATAGG TAGTATATTT GAAGTACTTT GGGCCATATT TAGACCAGGA
    ACTTCCTTTG GAATCAGTGT CTTAAGAGCT CTCCGACTTT TGAGAATCTT CAAAATAACA
    AAATACTGGG CCTCACTACG AAACCTTGTA GTATCATTGA TGAATTCTAT GAAATCCATC
    ATCAGCCTGC TCTTCCTTCT ATTCCTCTTC ATAGTTGTTT TTGCTCTCCT TGGAATGCAG
    TTATATGGTG GCAGATTCAA TTTTAATGAT GGCACTCCTT CTGCAAACTT TGATACATTC
    CCTGCAGCCA TCATGACTGT ATTCCAGATT CTGACAGGAG AGGATTGGAA TGAAGTGATG
    TACAATGGTA TTCGCTCCCA AGGTGGTGTT CGATCCGGAA TGTGGTCTTC CATATACTTT
    ATTGTCCTTA CCCTTTTTGG AAATTACACA CTGCTAAATG TATTTCTTGC CATTGCTGTG
    GACAACCTGG CTAATGCACA GGAGTTAACT AAGGATGAGC AAGAAGAAGA GGAAGCTTTC
    AATCAGAAGC ATGCACTGCA AAAAGCGAAG GAGGTCAGCC CCATGTCTGC ACCTAATGCA
    TCATGTATTG AGAAGGAGCG CAGAAAACGG CACACATTGT CTATATGGGA GCAGCGCACA
    AGCCAGCTAA GGAGGCACAT ACAGATGTCT AGTCAAGAAG CTCTCCAGAG TGAAGAACTG
    GCAGCATTGA ATCCACACTC CAGTATCTAT CGCAAGACAA AGGAAGCTGC TGAACAAAAC
    ACAGAAAAAG AAGAAGTGCA TCAAGACGCA AAACTGGCAA TAGACCCAGT TGAAGGACCA
    CCAGAAGGTG AAGGAAGCCC TTCTAATAGA AATCTCAGGG AAAACTGGCG CCAGAAGTCC
    AGGCATGGAA ATTCTGAAAA CACTGAGCCA AATGGAACAA CTCCAGAAGA AGCAATGCGC
    CGCAGACAAA GTCAGAGACG CAGCCGTCAT AGAAGAGTTA GAACTGAAGG TCGTGAGCAG
    TGTAGCAATC CAAACAGTAC CTCAGCAAGC AGAGAAGGTG GTCTTGACAA AGTCTCTGCA
    GAGGATGGAA GAACAATTCC AGAAGAGAAG AGCAATGGAG AAGCCAAAGA AGTCAAAGGG
    GTTGATGATG AGCCTCTAAA GATTTGTATA TCTTCAAGAA ATTGCGAGGA CAGAAATATC
    AGGACTTTGG GTTGTGAGGG TATTGCCTTG AACGATACAC CCCTGCAGGA TGTGAACAGC
    CAAGAGAAGG ACAGTAGCCC TCCTGATTTG GATTGCAGTG AGCAGGCTCT GTTGGGCAGC
    ATTCAGAGCC AGGAAAGCCC TAATCCAACC AGCTGCAGTT TGCCAGATGT GTCCAGCAGC
    ACCGAGAGAG TCACAGAGAG CACCGTCATT ACCTTACCAG ATACTGGAAT GCCCACCGAC
    TGCACCGTGG TACAGATGAG TGAAGGCAGG GATGGAGAAG TCAACTTTGG GTCTTCGCAG
    CAAGAAAAAG AAGAGGAGGA AGCTGAGGAT GGAGAAGAAA GAACTATAAA AAGAAAAGAG
    AAATTGTTGG AGTCCCCCAA AGCCATTGTA CCGTACAGTT CAATGCTCAT CTTCAGCTCC
    ACCAATCCCA TAAGAAAAGT ATGTCATTAC ATTGTTAACC TACGATACTT TGAGATGAGC
    ATCCTGCTGG TAATAGCAGC AAGTAGTATA GCCTTAGCAG CGGAGGACCC TGTACTGACC
    AACTCGGACC GCAACAAAGT TCTTCGCTAT TTTGACTATG TTTTCACAGG GGTCTTCACT
    TTTGAGATGA TTATAAAGAT GATTGACCAA GGTTTGATTT TACACGAGGG CTCCTACTTT
    CGTGACCTAT GGAACATCTT GGATTTTGTT GTTGTTGTGG GAGCCTTAAT TGCGTTTGCC
    TTAGCGACTA ACAAAGGCCG GGACATCAAA ACCATTAAGT CTCTACGTGT GTTACGGGTG
    CTGCGTCCAC TAAAAACAAT CAAACGCCTG CCAAAACTGA AGGCAGTCTT TGATTGTGTG
    GTTAACTCTT TAAAGAATGT CTTTAATATT CTCATCGTAT ACAAACTCTT CATGTTCATA
    TTTGCTGTAA TCGCTGTCCA GCTGTTTAAG GGCAAGTTCT TCTATTGTAC AGACAGCTCA
    AAGGAGACAG AGAAAGAGTG CATAGGGAGT TACATCGATC ACGAAAAGAA CAAGATGGAG
    GTGAAGCGGA GAGAGTGGAA AAGACATGAT TTCCATTATG ACAACATAAT ATGGGCTCTT
    CTTACCCTCT TTACTGTATC AACAGGGGAA GGCTGGCCTC AGGTTCTGCA GCACTCTGTT
    GATATAACTG AAGAAGATAG GGGTCCAAGC CGAAGCAACC GTATGGAGAT GTCCATTTTC
    TATGTTATCT ACTTTGTGGT CTTCCCCTTC TTCTTTGTAA ATATATTTGT GGCTCTGATT
    ATCATCACAT TCCAGGAGCA AGGAGACAAG ATGATGGAGG AATGTTCCCT AGAAAAGAAT
    GAGAGAGCCT GCATAGACTT TGCAATAAGT GCTAAGCCAC TTACACGATA TATGCCACAG
    AACAGGCACA CATTCCAGTA CCGGGTCTGG CACTTCGTGG TCTCCCCATC ATTTGAATAC
    ACTATCATGG CTATGATTGC ACTAAACACA GTGGTACTCA TGATGAAGTA TTATTCTGCG
    CCATACACAT ATGAGCTTGC ATTAAAGTAT CTCAATATTG CATTCACAAT GCTATTTTCT
    CTGGAGTGCA TCCTGAAAAT AATTGCATTT GGGTTTTTGA ATTACTTTCG GGATACTTGG
    AACATTTTTG ACTTCATTAC TGTAATTGGT AGCATCACGG AAATTATTCT AACAGATAGT
    AAGTTGGTGA ACACCAGCAG CTTTAATATG AGCTTTCTGA AGCTGTTCCG AGCGGCGCGA
    CTTATTAAAC TTCTCCGGCA AGGGTACACC ATCCGGATCC TGCTGTGGAC TTTTGTTCAG
    TCCTTTAAGG CACTTCCTTA TGTGTGTCTT CTCATTGCAA TGTTGTTCTT CATCTATGCC
    ATTATTGGGA TGCAGGTGTT TGGCAATATC CGCCTGGAAG AGGACAGTCA TATTAATAGG
    CACAACAATT TTAGAAGCTT CTTTAGTTCT CTTATGTTAC TCTTCAGGAG TGCCACAGGA
    GAAGCGTGGC AGGAGATCAT GCTTTCATGC CTTAGTGATA AGGGTTGTGA GCCAGACACC
    AGTGCAGCCT CTGGACACAA CGAGAATGAG CGCTGTGGAA CTGAACTTGC ATATGTTTAT
    TTTGTTTCAT TCATTTTCTT CTGCTCCTTC CTCATGCTAA ATCTGTTTGT GGCTGTCATT
    ATGGACAACT TTGAGTACTT GACACGGGAC TCTTCCATTT TGGGACCTCA TCACTTGGAT
    GAGTTTGTTC GGATATGGGC TGAATATGAT CGAGCAGCAT GTGGCCGTAT CCATTACACA
    GAGATGTATG ACATGCTGAC TCTCATGTCA CCACCTCTAG GCCTTGGAAA GAGATGCCCT
    AACAAAGTGG CTTATAAGAG ATTGGTCCTG ATGAATATGC CAGTGTCGGA GGACATGACG
    GTTCATTTCA CATCAACTCT TATGGCCCTA ATACGCACGG CACTGGATAT CAAAATAGCT
    AAAGGAGGTG CTGACAGGCA ACAGCTGGAC TCAGAGCTGC AGAAAGATAT ACTTGCCATT
    TGGCCAAATC TCTCCCAGAA AATGATCGAC TTACTTGTGC CAATGCCAAG AGCCTCTGAT
    CTGACGGTGG GGAAGATTTA CGCTGCAATG ATGATCATGG ATTACTATAA ACAGAGCAAA
    GCGAAAAAAC AAAGGCAGCA GCTGGAGGAG CAGAAAAATG CACCTATGTT TCAGAGAATG
    GAGGCTTCTG CCCTCCCTGA GGAGATCTTA TCCAATGCCA AAGCTTTACC ATACCTTCAG
    CAAGACACTC TATCAGGCCT GGGTGGATTT CCTTCACTGA GTCCCTTGTC CCCAGAAGAA
    GTATTCCAGC CGCCATGCCT AGACGGGGAA GAGGAGGAAG ACTTTGAAGA GCAGCTGTCT
    CTGGAGCCTG AGGTTAGAGA GTTTAGAAGG GAGCAGCTCC CTAACCGAGG CAGCTACATC
    CCTGTGGAGA TGCTGGAACG TCCAGCGTCT GTGAGAGCTT CTTCCATGCC ACGTCTGGCT
    GAGAATCCTC AGGTCCTGTC AGACGTAAGC TCCATGCGTC GCTCCTTCTC CACAACTGCA
    GAGAAGCGAG TGCGCAGCTC GTGGCTAGAT GAGTTCACTA TGGACAGGAG TAGCACTGAC
    TGTGCATACA GATACCAGAG GCGCAGTTAT CATGCATCCT TAAGACTGTC TGCACATCGC
    CTGAATACAG ATACAGGTCA CTGGTCAGAT AATCACCGTT CTGGCGGAAG GGAGCGAGGA
    AGGTCCAAAG AGAGAAAACA CTTGCTGTCA CCCGATCCTT CCCGCTGTAA TTCTGAGGAA
    AGGAGCCCAC AAGCTGCCTG TGAACACCGA GATCACCAAC TGTCCCCTGG GCATACTCCC
    AGTAGTGTAC AGATTCTTGA CTCTCTGAGT GAAAGTTGTA CCCCTTCAGA CACCAGCACC
    CCAAGAAGAG GCCGTAGACA GCTTCCTTCT GTGCCTCCAA CTCCTAGACC CCTTATCTCT
    TTCTCCTCGT TAATCCGCCG GCAGAAACAC AGCCCCCCTC CTCAAAATAA AAATGATACT
    ATCCTTCCTA GTGAGCCTGG TCTGACTGAG TCTTCCTGCT CCCTTGGGGG TCATCAGAGC
    TCCCAGTCCA CACCACAGCA TTATATCTCA GAGCCCTACC TGGCACTGCA TGAGGAGATG
    CAAAGCTCAG ACTGCCCAGC AGAGGACACA CTCACCTTTG AGGTTGCTGT GGCTACTAGT
    CTGGGCCGCT CGAACACCAT TGGCTCTGCC CCTCTACGTC ATGGCTGGCA AATGCCCAAT
    GGTCACTACC GGCGTCGAAG GAAGGGAGGA ATTCCACCAG TGATACCCTC CAGAGAGTTA
    AGTGACACAG AAGAGGATGA TCGATGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031756840.1
    CDS285..7454
    Translation

    Target ORF information:

    RefSeq Version XM_031900980.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calcium channel, voltage-dependent, R type, alpha 1E subunit (cacna1e), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031900980.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGGTATTAA AGATGCTGGG ATTAAGCTCC TGTTCAACCA GTGCTCATAC ACTCAATGAG 
    CCTGACCAGT CAAGCACACT AGCCATACCA GCATTGGATG CCGACACTCA GAGATATGTC
    TTGGCCCTTC CAGAAGCAGA GACATGGCGC AAGCATCGAT TGGGCCTGAC ACAGGCTACT
    CACTCTTGCA ATCTTCTAGA ACCTGAGACA CTTTCTGAAA CCCTAGTTTC ACGCACTGCA
    AGTTACGATG CCTTGTATGA CCTGCGAGCA AGAGAAGCGG AGTCTAACTT CCCATTTGAA
    GGCAGCAGCA TGTTTGAGGT GACGTCTGGG ATGGATTCTG AGTTTCCTTT AAGCCCCCAT
    GTTATAAAGC GAAGGAGAGG AGGCCTGATA GAACAAAAGG ATATCATCAA GGCCCACGAA
    GCTCATAAGA TTCAGAGTAC TCCCCAGGCT CGACGGAAGG AATGGGAGAT GGCTCGTTTC
    GGAGAGGCTG TGACTGGCAG GTTGAGCTCC GCAGATGGGG GCTCAGAGAG GAGCAAGACC
    CTACAAGGTT CCCCAGCTGC ACCTGTTGGG ACCCAAGCAG CTTTCAAGCA ATCCAAAGCC
    CAGAGGGCAA GGACCATGGC TTTATACAAC CCCATCCCTG TCCGACAGAA CTGCTTCACC
    GTGAACAGGT CTCTCTTCAT TTTCGGAGAA GATAATATTA TCAGGAAATA CGCCAAGAAG
    CTCATTGATT GGCCTCCTTT TGAATACATG ATCCTTGCCA CAATTATTGC AAACTGCATT
    GTCCTGGCTT TGGAGCAGCA TCTTCCAGGC GATGACAAGA CTCCAATGTC TCGCAGATTG
    GAAAAGACTG AGCCCTATTT CATTGGGATA TTTTGCTTTG AAGCTGGAAT TAAAATTGTC
    GCTCTGGGAC TGGTTTTTCA CAAAGGTTCT TATCTTCGGA ATGGCTGGAA TGTCATGGAC
    TTTATAGTAG TGCTCAGTGG CATCCTGGCC ACAGCTGGAA CTCACTTCAA TACTCACGTG
    GACCTGCGTA CTTTGCGGGC AGTGCGTGTT CTCAGGCCGT TGAAGCTGGT CTCGGGGATT
    CCCAGTTTAC AGATTGTGTT GAAGTCAATC ATGAAGGCGA TGGTCCCTCT GCTACAGATT
    GGATTGTTGC TCTTCTTTGC CATCCTTATG TTTGCTATCA TCGGCCTTGA GTTTTACAGT
    GGAAAGCTAC ACAAGGCCTG TTACAGGAAC AACTCTGGAA TGGAGCTAGA CCCCATGGAT
    CCACCTCACC CTTGTGGAGT GCCCCGCTGC CCAGCTGGGT ATGAGTGCAA GGAATGGATC
    GGACCCAATG ATGGAATAAC ACAGTTTGAC AACATCCTAT TTGCGGTGCT GACAGTCTTC
    CAGTGTATAA CTATGGAGGG CTGGACCACA GTGCTGTACA ATACCAATGA TGCCTTAGGA
    GCCACATGGA ACTGGCTGTA CTTCATTCCC CTCATTATCA TCGGATCCTT CTTTGTCCTT
    AATCTTGTCC TTGGAGTGCT GTCCGGTGAA TTTGCCAAAG AGAGGGAAAG AGTGGAAAAC
    CGCCGGGCTT TCATGAAGTT GCGCAGGCAG CAGCAGATTG AGAGGGAGTT GAATGGCTAT
    CGGGCATGGA TAGATAAAGC TGAGGAAGTC ATGTTGGCAG AAGAAAACAA AAATTCTGGG
    ACCTCTGCTT TAGAAGTTCT TAGGCGAGCA ACCATTAAAC GAAGTAGAAC AGAAGCCATG
    AACCGGGACT CTACTGAGGA CCATTGTGTT GACATATCCT CTGTTGGCAC CCCCCTTGCT
    AGAGCAAGCA TCAAAAGTAC CAAAATGGAT GGGGCATCAT ATTTTCGGCA CAAGGAAAAA
    CTATTGCGTG TCTCCGTTCG TCACATGATC AAATCCCAGG TTTTCTACTG GATGGTCCTG
    GGAGTGGTAG CACTTAACAC TTTCTGTGTG GCTATTGTCC ATCACAACCA GACCCTGTGG
    CTCTCAAATG TTCTTTACTA TGCAGAATTT CTGTTTCTTG GGTTATTTTT GGTGGAGATG
    GCTTTTAAAA TGTATGGAAT GGGTCCACGT CTTTACTTTC ACTCATCCTT CAACTGCTTT
    GATTGCGGGG TCACAATAGG TAGTATATTT GAAGTACTTT GGGCCATATT TAGACCAGGA
    ACTTCCTTTG GAATCAGTGT CTTAAGAGCT CTCCGACTTT TGAGAATCTT CAAAATAACA
    AAATACTGGG CCTCACTACG AAACCTTGTA GTATCATTGA TGAATTCTAT GAAATCCATC
    ATCAGCCTGC TCTTCCTTCT ATTCCTCTTC ATAGTTGTTT TTGCTCTCCT TGGAATGCAG
    TTATATGGTG GCAGATTCAA TTTTAATGAT GGCACTCCTT CTGCAAACTT TGATACATTC
    CCTGCAGCCA TCATGACTGT ATTCCAGATT CTGACAGGAG AGGATTGGAA TGAAGTGATG
    TACAATGGTA TTCGCTCCCA AGGTGGTGTT CGATCCGGAA TGTGGTCTTC CATATACTTT
    ATTGTCCTTA CCCTTTTTGG AAATTACACA CTGCTAAATG TATTTCTTGC CATTGCTGTG
    GACAACCTGG CTAATGCACA GGAGTTAACT AAGGATGAGC AAGAAGAAGA GGAAGCTTTC
    AATCAGAAGC ATGCACTGCA AAAAGCGAAG GAGGTCAGCC CCATGTCTGC ACCTAATGCA
    TCATGTATTG AGAAGGAGCG CAGAAAACGG CACACATTGT CTATATGGGA GCAGCGCACA
    AGCCAGCTAA GGAGGCACAT ACAGATGTCT AGTCAAGAAG CTCTCCAGAG TGAAGAACTG
    GCAGCATTGA ATCCACACTC CAGTATCTAT CGCAAGACAA AGGAAGCTGC TGAACAAAAC
    ACAGAAAAAG AAGAAGTGCA TCAAGACGCA AAACTGGCAA TAGACCCAGT TGAAGGACCA
    CCAGAAGGTG AAGGAAGCCC TTCTAATAGA AATCTCAGGG AAAACTGGCG CCAGAAGTCC
    AGGCATGGAA ATTCTGAAAA CACTGAGCCA AATGGAACAA CTCCAGAAGA AGCAATGCGC
    CGCAGACAAA GTCAGAGACG CAGCCGTCAT AGAAGAGTTA GAACTGAAGG TCGTGAGCAG
    TGTAGCAATC CAAACAGTAC CTCAGCAAGC AGAGAAGGTG GTCTTGACAA AGTCTCTGCA
    GAGGATGGAA GAACAATTCC AGAAGAGAAG AGCAATGGAG AAGCCAAAGA AGTCAAAGGG
    GTTGATGATG AGCCTCTAAA GATTTGTATA TCTTCAAGAA ATTGCGAGGA CAGAAATATC
    AGGACTTTGG GTTGTGAGGG TATTGCCTTG AACGATACAC CCCTGCAGGA TGTGAACAGC
    CAAGAGAAGG ACAGTAGCCC TCCTGATTTG GATTGCAGTG AGCAGGCTCT GTTGGGCAGC
    ATTCAGAGCC AGGAAAGCCC TAATCCAACC AGCTGCAGTT TGCCAGATGT GTCCAGCAGC
    ACCGAGAGAG TCACAGAGAG CACCGTCATT ACCTTACCAG ATACTGGAAT GCCCACCGAC
    TGCACCGTGG TACAGATGAG TGAAGGCAGG GATGGAGAAG TCAACTTTGG GTCTTCGCAG
    CAAGAAAAAG AAGAGGAGGA AGCTGAGGAT GGAGAAGAAA GAACTATAAA AAGAAAAGAG
    AAATTGTTGG AGTCCCCCAA AGCCATTGTA CCGTACAGTT CAATGCTCAT CTTCAGCTCC
    ACCAATCCCA TAAGAAAAGT ATGTCATTAC ATTGTTAACC TACGATACTT TGAGATGAGC
    ATCCTGCTGG TAATAGCAGC AAGTAGTATA GCCTTAGCAG CGGAGGACCC TGTACTGACC
    AACTCGGACC GCAACAAAGT TCTTCGCTAT TTTGACTATG TTTTCACAGG GGTCTTCACT
    TTTGAGATGA TTATAAAGAT GATTGACCAA GGTTTGATTT TACACGAGGG CTCCTACTTT
    CGTGACCTAT GGAACATCTT GGATTTTGTT GTTGTTGTGG GAGCCTTAAT TGCGTTTGCC
    TTAGCGACTA ACAAAGGCCG GGACATCAAA ACCATTAAGT CTCTACGTGT GTTACGGGTG
    CTGCGTCCAC TAAAAACAAT CAAACGCCTG CCAAAACTGA AGGCAGTCTT TGATTGTGTG
    GTTAACTCTT TAAAGAATGT CTTTAATATT CTCATCGTAT ACAAACTCTT CATGTTCATA
    TTTGCTGTAA TCGCTGTCCA GCTGTTTAAG GGCAAGTTCT TCTATTGTAC AGACAGCTCA
    AAGGAGACAG AGAAAGAGTG CATAGGGAGT TACATCGATC ACGAAAAGAA CAAGATGGAG
    GTGAAGCGGA GAGAGTGGAA AAGACATGAT TTCCATTATG ACAACATAAT ATGGGCTCTT
    CTTACCCTCT TTACTGTATC AACAGGGGAA GGCTGGCCTC AGGTTCTGCA GCACTCTGTT
    GATATAACTG AAGAAGATAG GGGTCCAAGC CGAAGCAACC GTATGGAGAT GTCCATTTTC
    TATGTTATCT ACTTTGTGGT CTTCCCCTTC TTCTTTGTAA ATATATTTGT GGCTCTGATT
    ATCATCACAT TCCAGGAGCA AGGAGACAAG ATGATGGAGG AATGTTCCCT AGAAAAGAAT
    GAGAGAGCCT GCATAGACTT TGCAATAAGT GCTAAGCCAC TTACACGATA TATGCCACAG
    AACAGGCACA CATTCCAGTA CCGGGTCTGG CACTTCGTGG TCTCCCCATC ATTTGAATAC
    ACTATCATGG CTATGATTGC ACTAAACACA GTGGTACTCA TGATGAAGTA TTATTCTGCG
    CCATACACAT ATGAGCTTGC ATTAAAGTAT CTCAATATTG CATTCACAAT GCTATTTTCT
    CTGGAGTGCA TCCTGAAAAT AATTGCATTT GGGTTTTTGA ATTACTTTCG GGATACTTGG
    AACATTTTTG ACTTCATTAC TGTAATTGGT AGCATCACGG AAATTATTCT AACAGATAGT
    AAGTTGGTGA ACACCAGCAG CTTTAATATG AGCTTTCTGA AGCTGTTCCG AGCGGCGCGA
    CTTATTAAAC TTCTCCGGCA AGGGTACACC ATCCGGATCC TGCTGTGGAC TTTTGTTCAG
    TCCTTTAAGG CACTTCCTTA TGTGTGTCTT CTCATTGCAA TGTTGTTCTT CATCTATGCC
    ATTATTGGGA TGCAGGTGTT TGGCAATATC CGCCTGGAAG AGGACAGTCA TATTAATAGG
    CACAACAATT TTAGAAGCTT CTTTAGTTCT CTTATGTTAC TCTTCAGGAG TGCCACAGGA
    GAAGCGTGGC AGGAGATCAT GCTTTCATGC CTTAGTGATA AGGGTTGTGA GCCAGACACC
    AGTGCAGCCT CTGGACACAA CGAGAATGAG CGCTGTGGAA CTGAACTTGC ATATGTTTAT
    TTTGTTTCAT TCATTTTCTT CTGCTCCTTC CTCATGCTAA ATCTGTTTGT GGCTGTCATT
    ATGGACAACT TTGAGTACTT GACACGGGAC TCTTCCATTT TGGGACCTCA TCACTTGGAT
    GAGTTTGTTC GGATATGGGC TGAATATGAT CGAGCAGCAT GTGGCCGTAT CCATTACACA
    GAGATGTATG ACATGCTGAC TCTCATGTCA CCACCTCTAG GCCTTGGAAA GAGATGCCCT
    AACAAAGTGG CTTATAAGAG ATTGGTCCTG ATGAATATGC CAGTGTCGGA GGACATGACG
    GTTCATTTCA CATCAACTCT TATGGCCCTA ATACGCACGG CACTGGATAT CAAAATAGCT
    AAAGGAGGTG CTGACAGGCA ACAGCTGGAC TCAGAGCTGC AGAAAGATAT ACTTGCCATT
    TGGCCAAATC TCTCCCAGAA AATGATCGAC TTACTTGTGC CAATGCCAAG AGCCTCTGAT
    CTGACGGTGG GGAAGATTTA CGCTGCAATG ATGATCATGG ATTACTATAA ACAGAGCAAA
    GCGAAAAAAC AAAGGCAGCA GCTGGAGGAG CAGAAAAATG CACCTATGTT TCAGAGAATG
    GAGGCTTCTG CCCTCCCTGA GGAGATCTTA TCCAATGCCA AAGCTTTACC ATACCTTCAG
    CAAGACACTC TATCAGGCCT GGGTGGATTT CCTTCACTGA GTCCCTTGTC CCCAGAAGAA
    GTATTCCAGC CGCCATGCCT AGACGGGGAA GAGGAGGAAG ACTTTGAAGA GCAGCTGTCT
    CTGGAGCCTG AGGTTAGAGA GTTTAGAAGG GAGCAGCTCC CTAACCGAGG CAGCTACATC
    CCTGTGGAGA TGCTGGAACG TCCAGCGTCT GTGAGAGCTT CTTCCATGCC ACGTCTGGCT
    GAGAATCCTC AGGTCCTGTC AGACGTAAGC TCCATGCGTC GCTCCTTCTC CACAACTGCA
    GAGAAGCGAG TGCGCAGCTC GTGGCTAGAT GAGTTCACTA TGGACAGGAG TAGCACTGAC
    TGTGCATACA GATACCAGAG GCGCAGTTAT CATGCATCCT TAAGACTGTC TGCACATCGC
    CTGAATACAG ATACAGGTCA CTGGTCAGAT AATCACCGTT CTGGCGGAAG GGAGCGAGGA
    AGGTCCAAAG AGAGAAAACA CTTGCTGTCA CCCGATCCTT CCCGCTGTAA TTCTGAGGAA
    AGGAGCCCAC AAGCTGCCTG TGAACACCGA GATCACCAAC TGTCCCCTGG GCATACTCCC
    AGTAGTGTAC AGATTCTTGA CTCTCTGAGT GAAAGTTGTA CCCCTTCAGA CACCAGCACC
    CCAAGAAGAG GCCGTAGACA GCTTCCTTCT GTGCCTCCAA CTCCTAGACC CCTTATCTCT
    TTCTCCTCGT TAATCCGCCG GCAGAAACAC AGCCCCCCTC CTCAAAATAA AAATGATACT
    ATCCTTCCTA GTGAGCCTGG TCTGACTGAG TCTTCCTGCT CCCTTGGGGG TCATCAGAGC
    TCCCAGTCCA CACCACAGCA TTATATCTCA GAGCCCTACC TGGCACTGCA TGAGGAGATG
    CAAAGCTCAG ACTGCCCAGC AGAGGACACA CTCACCTTTG AGGTTGCTGT GGCTACTAGT
    CTGGGCCGCT CGAACACCAT TGGCTCTGCC CCTCTACGTC ATGGCTGGCA AATGCCCAAT
    GGTCACTACC GGCGTCGAAG GAAGGGAGGA ATTCCACCAG TGATACCCTC CAGAGAGTTA
    AGTGACACAG AAGAGGATGA TCGATGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa57232
    Clone ID Related Accession (Same CDS sequence) XM_031900981.1
    Accession Version XM_031900981.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7053bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGTATTAA AGATGCTGGG ATTAAGCTCC TGTTCAACCA GTGCTCATAC ACTCAATGAG 
    CCTGACCAGT CAAGCACACT AGCCATACCA GCATTGGATG CCGACACTCA GAGATATGTC
    TTGGCCCTTC CAGAAGCAGA GACATGGCGC AAGCATCGAT TGGGCCTGAC ACAGGCTACT
    CACTCTTGCA ATCTTCTAGA ACCTGAGACA CTTTCTGAAA CCCTAGTTTC ACGCACTGCA
    AGTTACGATG CCTTGTATGA CCTGCGAGCA AGAGAAGCGG AGTCTAACTT CCCATTTGAA
    GGCAGCAGCA TGTTTGAGGT GACGTCTGGG ATGGATTCTG AGTTTCCTTT AAGCCCCCAT
    GTTATAAAGC GAAGGAGAGG AGGCCTGATA GAACAAAAGG ATATCATCAA GGCCCACGAA
    GCTCATAAGA TTCAGAGTAC TCCCCAGGCT CGACGGAAGG AATGGGAGAT GGCTCGTTTC
    GGAGAGGCTG TGACTGGCAG GTTGAGCTCC GCAGATGGGG GCTCAGAGAG GAGCAAGACC
    CTACAAGGTT CCCCAGCTGC ACCTGTTGGG ACCCAAGCAG CTTTCAAGCA ATCCAAAGCC
    CAGAGGGCAA GGACCATGGC TTTATACAAC CCCATCCCTG TCCGACAGAA CTGCTTCACC
    GTGAACAGGT CTCTCTTCAT TTTCGGAGAA GATAATATTA TCAGGAAATA CGCCAAGAAG
    CTCATTGATT GGCCTCCTTT TGAATACATG ATCCTTGCCA CAATTATTGC AAACTGCATT
    GTCCTGGCTT TGGAGCAGCA TCTTCCAGGC GATGACAAGA CTCCAATGTC TCGCAGATTG
    GAAAAGACTG AGCCCTATTT CATTGGGATA TTTTGCTTTG AAGCTGGAAT TAAAATTGTC
    GCTCTGGGAC TGGTTTTTCA CAAAGGTTCT TATCTTCGGA ATGGCTGGAA TGTCATGGAC
    TTTATAGTAG TGCTCAGTGG CATCCTGGCC ACAGCTGGAA CTCACTTCAA TACTCACGTG
    GACCTGCGTA CTTTGCGGGC AGTGCGTGTT CTCAGGCCGT TGAAGCTGGT CTCGGGGATT
    CCCAGTTTAC AGATTGTGTT GAAGTCAATC ATGAAGGCGA TGGTCCCTCT GCTACAGATT
    GGATTGTTGC TCTTCTTTGC CATCCTTATG TTTGCTATCA TCGGCCTTGA GTTTTACAGT
    GGAAAGCTAC ACAAGGCCTG TTACAGGAAC AACTCTGGAA TGGAGCTAGA CCCCATGGAT
    CCACCTCACC CTTGTGGAGT GCCCCGCTGC CCAGCTGGGT ATGAGTGCAA GGAATGGATC
    GGACCCAATG ATGGAATAAC ACAGTTTGAC AACATCCTAT TTGCGGTGCT GACAGTCTTC
    CAGTGTATAA CTATGGAGGG CTGGACCACA GTGCTGTACA ATACCAATGA TGCCTTAGGA
    GCCACATGGA ACTGGCTGTA CTTCATTCCC CTCATTATCA TCGGATCCTT CTTTGTCCTT
    AATCTTGTCC TTGGAGTGCT GTCCGGTGAA TTTGCCAAAG AGAGGGAAAG AGTGGAAAAC
    CGCCGGGCTT TCATGAAGTT GCGCAGGCAG CAGCAGATTG AGAGGGAGTT GAATGGCTAT
    CGGGCATGGA TAGATAAAGC TGAGGAAGTC ATGTTGGCAG AAGAAAACAA AAATTCTGGG
    ACCTCTGCTT TAGAAGTTCT TAGGCGAGCA ACCATTAAAC GAAGTAGAAC AGAAGCCATG
    AACCGGGACT CTACTGAGGA CCATTGTGTT GACATATCCT CTGTTGGCAC CCCCCTTGCT
    AGAGCAAGCA TCAAAAGTAC CAAAATGGAT GGGGCATCAT ATTTTCGGCA CAAGGAAAAA
    CTATTGCGTG TCTCCGTTCG TCACATGATC AAATCCCAGG TTTTCTACTG GATGGTCCTG
    GGAGTGGTAG CACTTAACAC TTTCTGTGTG GCTATTGTCC ATCACAACCA GACCCTGTGG
    CTCTCAAATG TTCTTTACTA TGCAGAATTT CTGTTTCTTG GGTTATTTTT GGTGGAGATG
    GCTTTTAAAA TGTATGGAAT GGGTCCACGT CTTTACTTTC ACTCATCCTT CAACTGCTTT
    GATTGCGGGG TCACAATAGG TAGTATATTT GAAGTACTTT GGGCCATATT TAGACCAGGA
    ACTTCCTTTG GAATCAGTGT CTTAAGAGCT CTCCGACTTT TGAGAATCTT CAAAATAACA
    AAATACTGGG CCTCACTACG AAACCTTGTA GTATCATTGA TGAATTCTAT GAAATCCATC
    ATCAGCCTGC TCTTCCTTCT ATTCCTCTTC ATAGTTGTTT TTGCTCTCCT TGGAATGCAG
    TTATATGGTG GCAGATTCAA TTTTAATGAT GGCACTCCTT CTGCAAACTT TGATACATTC
    CCTGCAGCCA TCATGACTGT ATTCCAGATT CTGACAGGAG AGGATTGGAA TGAAGTGATG
    TACAATGGTA TTCGCTCCCA AGGTGGTGTT CGATCCGGAA TGTGGTCTTC CATATACTTT
    ATTGTCCTTA CCCTTTTTGG AAATTACACA CTGCTAAATG TATTTCTTGC CATTGCTGTG
    GACAACCTGG CTAATGCACA GGAGTTAACT AAGGATGAGC AAGAAGAAGA GGAAGCTTTC
    AATCAGAAGC ATGCACTGCA AAAAGCGAAG GAGGTCAGCC CCATGTCTGC ACCTAATGCA
    TCATGTATTG AGAAGGAGCG CAGAAAACGG CACACATTGT CTATATGGGA GCAGCGCACA
    AGCCAGCTAA GGAGGCACAT ACAGATGTCT AGTCAAGAAG CTCTCCAGAG TGAAGAACTG
    GCAGCATTGA ATCCACACTC CAGTATCTAT CGCAAGACAA AGGAAGCTGC TGAACAAAAC
    ACAGAAAAAG AAGAAGTGCA TCAAGACGCA AAACTGGCAA TAGACCCAGT TGAAGGACCA
    CCAGAAGGTG AAGGAAGCCC TTCTAATAGA AATCTCAGGG AAAACTGGCG CCAGAAGTCC
    AGGCATGGAA ATTCTGAAAA CACTGAGCCA AATGGAACAA CTCCAGAAGA AGCAATGCGC
    CGCAGACAAA GTCAGAGACG CAGCCGTCAT AGAAGAGTTA GAACTGAAGG TCGTGAGCAG
    TGTAGCAATC CAAACAGTAC CTCAGCAAGC AGAGAAGGTG GTCTTGACAA AGTCTCTGCA
    GAGGATGGAA GAACAATTCC AGAAGAGAAG AGCAATGGAG AAGCCAAAGA AGTCAAAGGG
    GTTGATGATG AGCCTCTAAA GATTTGTATA TCTTCAAGAA ATTGCGAGGA CAGAAATATC
    AGGACTTTGG GTTGTGAGGG TATTGCCTTG AACGATACAC CCCTGCAGGA TGTGAACAGC
    CAAGAGAAGG ACAGTAGCCC TCCTGATTTG GATTGCAGTG AGCAGGCTCT GTTGGGCAGC
    ATTCAGAGCC AGGAAAGCCC TAATCCAACC AGCTGCAGTT TGCCAGATGT GTCCAGCAGC
    ACCGAGAGAG TCACAGAGAG CACCGTCATT ACCTTACCAG ATACTGGAAT GCCCACCGAC
    TGCACCGTGG TACAGATGAG TGAAGGCAGG GATGGAGAAG TCAACTTTGG GTCTTCGCAG
    CAAGAAAAAG AAGAGGAGGA AGCTGAGGAT GGAGAAGAAA GAACTATAAA AAGAAAAGAG
    AAATTGTTGG AGTCCCCCAA AGCCATTGTA CCGTACAGTT CAATGCTCAT CTTCAGCTCC
    ACCAATCCCA TAAGAAAAGT ATGTCATTAC ATTGTTAACC TACGATACTT TGAGATGAGC
    ATCCTGCTGG TAATAGCAGC AAGTAGTATA GCCTTAGCAG CGGAGGACCC TGTACTGACC
    AACTCGGACC GCAACAAAGT TCTTCGCTAT TTTGACTATG TTTTCACAGG GGTCTTCACT
    TTTGAGATGA TTATAAAGAT GATTGACCAA GGTTTGATTT TACACGAGGG CTCCTACTTT
    CGTGACCTAT GGAACATCTT GGATTTTGTT GTTGTTGTGG GAGCCTTAAT TGCGTTTGCC
    TTAGCGAATG TTTTGGGGAC TAACAAAGGC CGGGACATCA AAACCATTAA GTCTCTACGT
    GTGTTACGGG TGCTGCGTCC ACTAAAAACA ATCAAACGCC TGCCAAAACT GAAGGCAGTC
    TTTGATTGTG TGGTTAACTC TTTAAAGAAT GTCTTTAATA TTCTCATCGT ATACAAACTC
    TTCATGTTCA TATTTGCTGT AATCGCTGTC CAGCTGTTTA AGGGCAAGTT CTTCTATTGT
    ACAGACAGCT CAAAGGAGAC AGAGAAAGAG TGCATAGGGA GTTACATCGA TCACGAAAAG
    AACAAGATGG AGGTGAAGCG GAGAGAGTGG AAAAGACATG ATTTCCATTA TGACAACATA
    ATATGGGCTC TTCTTACCCT CTTTACTGTA TCAACAGGGG AAGGCTGGCC TCAGGTTCTG
    CAGCACTCTG TTGATATAAC TGAAGAAGAT AGGGGTCCAA GCCGAAGCAA CCGTATGGAG
    ATGTCCATTT TCTATGTTAT CTACTTTGTG GTCTTCCCCT TCTTCTTTGT AAATATATTT
    GTGGCTCTGA TTATCATCAC ATTCCAGGAG CAAGGAGACA AGATGATGGA GGAATGTTCC
    CTAGAAAAGA ATGAGAGAGC CTGCATAGAC TTTGCAATAA GTGCTAAGCC ACTTACACGA
    TATATGCCAC AGAACAGGCA CACATTCCAG TACCGGGTCT GGCACTTCGT GGTCTCCCCA
    TCATTTGAAT ACACTATCAT GGCTATGATT GCACTAAACA CAGTGGTACT CATGATGAAG
    TATTATTCTG CGCCATACAC ATATGAGCTT GCATTAAAGT ATCTCAATAT TGCATTCACA
    ATGCTATTTT CTCTGGAGTG CATCCTGAAA ATAATTGCAT TTGGGTTTTT GAATTACTTT
    CGGGATACTT GGAACATTTT TGACTTCATT ACTGTAATTG GTAGCATCAC GGAAATTATT
    CTAACAGATA GTAAGTTGGT GAACACCAGC AGCTTTAATA TGAGCTTTCT GAAGCTGTTC
    CGAGCGGCGC GACTTATTAA ACTTCTCCGG CAAGGGTACA CCATCCGGAT CCTGCTGTGG
    ACTTTTGTTC AGTCCTTTAA GGCACTTCCT TATGTGTGTC TTCTCATTGC AATGTTGTTC
    TTCATCTATG CCATTATTGG GATGCAGGTG TTTGGCAATA TCCGCCTGGA AGAGGACAGT
    CATATTAATA GGCACAACAA TTTTAGAAGC TTCTTTAGTT CTCTTATGTT ACTCTTCAGG
    AGTGCCACAG GAGAAGCGTG GCAGGAGATC ATGCTTTCAT GCCTTAGTGA TAAGGGTTGT
    GAGCCAGACA CCAGTGCAGC CTCTGGACAC AACGAGAATG AGCGCTGTGG AACTGAACTT
    GCATATGTTT ATTTTGTTTC ATTCATTTTC TTCTGCTCCT TCCTCATGCT AAATCTGTTT
    GTGGCTGTCA TTATGGACAA CTTTGAGTAC TTGACACGGG ACTCTTCCAT TTTGGGACCT
    CATCACTTGG ATGAGTTTGT TCGGATATGG GCTGAATATG ATCGAGCAGC ATGTGGCCGT
    ATCCATTACA CAGAGATGTA TGACATGCTG ACTCTCATGT CACCACCTCT AGGCCTTGGA
    AAGAGATGCC CTAACAAAGT GGCTTATAAG AGATTGGTCC TGATGAATAT GCCAGTGTCG
    GAGGACATGA CGGTTCATTT CACATCAACT CTTATGGCCC TAATACGCAC GGCACTGGAT
    ATCAAAATAG CTAAAGGAGG TGCTGACAGG CAACAGCTGG ACTCAGAGCT GCAGAAAGAT
    ATACTTGCCA TTTGGCCAAA TCTCTCCCAG AAAATGATCG ACTTACTTGT GCCAATGCCA
    AGAGCCTCTG ATCTGACGGT GGGGAAGATT TACGCTGCAA TGATGATCAT GGATTACTAT
    AAACAGAGCA AAGCGAAAAA ACAAAGGCAG CAGCTGGAGG AGCAGAAAAA TGCACCTATG
    TTTCAGAGAA TGGAGGCTTC TGCCCTCCCT GAGGAGATCT TATCCAATGC CAAAGCTTTA
    CCATACCTTC AGCAAGACAC TCTATCAGGC CTGGGTGGAT TTCCTTCACT GAGTCCCTTG
    TCCCCAGAAG AAGTATTCCA GCCGCCATGC CTAGACGGGG AAGAGGAGGA AGACTTTGAA
    GAGCAGCTGT CTCTGGTCCT GTCAGACGTA AGCTCCATGC GTCGCTCCTT CTCCACAACT
    GCAGAGAAGC GAGTGCGCAG CTCGTGGCTA GATGAGTTCA CTATGGACAG GAGTAGCACT
    GACTGTGCAT ACAGATACCA GAGGCGCAGT TATCATGCAT CCTTAAGACT GTCTGCACAT
    CGCCTGAATA CAGATACAGG TCACTGGTCA GATAATCACC GTTCTGGCGG AAGGGAGCGA
    GGAAGGTCCA AAGAGAGAAA ACACTTGCTG TCACCCGATC CTTCCCGCTG TAATTCTGAG
    GAAAGGAGCC CACAAGCTGC CTGTGAACAC CGAGATCACC AACTGTCCCC TGGGCATACT
    CCCAGTAGTG TACAGATTCT TGACTCTCTG AGTGAAAGTT GTACCCCTTC AGACACCAGC
    ACCCCAAGAA GAGGCCGTAG ACAGCTTCCT TCTGTGCCTC CAACTCCTAG ACCCCTTATC
    TCTTTCTCCT CGTTAATCCG CCGGCAGAAA CACAGCCCCC CTCCTCAAAA TAAAAATGAT
    ACTATCCTTC CTAGTGAGCC TGGTCTGACT GAGTCTTCCT GCTCCCTTGG GGGTCATCAG
    AGCTCCCAGT CCACACCACA GCATTATATC TCAGAGCCCT ACCTGGCACT GCATGAGGAG
    ATGCAAAGCT CAGACTGCCC AGCAGAGGAC ACACTCACCT TTGAGGTTGC TGTGGCTACT
    AGTCTGGGCC GCTCGAACAC CATTGGCTCT GCCCCTCTAC GTCATGGCTG GCAAATGCCC
    AATGGTCACT ACCGGCGTCG AAGGAAGGGA GGAATTCCAC CAGTGATACC CTCCAGAGAG
    TTAAGTGACA CAGAAGAGGA TGATCGATGT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031756841.1
    CDS284..7336
    Translation

    Target ORF information:

    RefSeq Version XM_031900981.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calcium channel, voltage-dependent, R type, alpha 1E subunit (cacna1e), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031900981.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGTATTAA AGATGCTGGG ATTAAGCTCC TGTTCAACCA GTGCTCATAC ACTCAATGAG 
    CCTGACCAGT CAAGCACACT AGCCATACCA GCATTGGATG CCGACACTCA GAGATATGTC
    TTGGCCCTTC CAGAAGCAGA GACATGGCGC AAGCATCGAT TGGGCCTGAC ACAGGCTACT
    CACTCTTGCA ATCTTCTAGA ACCTGAGACA CTTTCTGAAA CCCTAGTTTC ACGCACTGCA
    AGTTACGATG CCTTGTATGA CCTGCGAGCA AGAGAAGCGG AGTCTAACTT CCCATTTGAA
    GGCAGCAGCA TGTTTGAGGT GACGTCTGGG ATGGATTCTG AGTTTCCTTT AAGCCCCCAT
    GTTATAAAGC GAAGGAGAGG AGGCCTGATA GAACAAAAGG ATATCATCAA GGCCCACGAA
    GCTCATAAGA TTCAGAGTAC TCCCCAGGCT CGACGGAAGG AATGGGAGAT GGCTCGTTTC
    GGAGAGGCTG TGACTGGCAG GTTGAGCTCC GCAGATGGGG GCTCAGAGAG GAGCAAGACC
    CTACAAGGTT CCCCAGCTGC ACCTGTTGGG ACCCAAGCAG CTTTCAAGCA ATCCAAAGCC
    CAGAGGGCAA GGACCATGGC TTTATACAAC CCCATCCCTG TCCGACAGAA CTGCTTCACC
    GTGAACAGGT CTCTCTTCAT TTTCGGAGAA GATAATATTA TCAGGAAATA CGCCAAGAAG
    CTCATTGATT GGCCTCCTTT TGAATACATG ATCCTTGCCA CAATTATTGC AAACTGCATT
    GTCCTGGCTT TGGAGCAGCA TCTTCCAGGC GATGACAAGA CTCCAATGTC TCGCAGATTG
    GAAAAGACTG AGCCCTATTT CATTGGGATA TTTTGCTTTG AAGCTGGAAT TAAAATTGTC
    GCTCTGGGAC TGGTTTTTCA CAAAGGTTCT TATCTTCGGA ATGGCTGGAA TGTCATGGAC
    TTTATAGTAG TGCTCAGTGG CATCCTGGCC ACAGCTGGAA CTCACTTCAA TACTCACGTG
    GACCTGCGTA CTTTGCGGGC AGTGCGTGTT CTCAGGCCGT TGAAGCTGGT CTCGGGGATT
    CCCAGTTTAC AGATTGTGTT GAAGTCAATC ATGAAGGCGA TGGTCCCTCT GCTACAGATT
    GGATTGTTGC TCTTCTTTGC CATCCTTATG TTTGCTATCA TCGGCCTTGA GTTTTACAGT
    GGAAAGCTAC ACAAGGCCTG TTACAGGAAC AACTCTGGAA TGGAGCTAGA CCCCATGGAT
    CCACCTCACC CTTGTGGAGT GCCCCGCTGC CCAGCTGGGT ATGAGTGCAA GGAATGGATC
    GGACCCAATG ATGGAATAAC ACAGTTTGAC AACATCCTAT TTGCGGTGCT GACAGTCTTC
    CAGTGTATAA CTATGGAGGG CTGGACCACA GTGCTGTACA ATACCAATGA TGCCTTAGGA
    GCCACATGGA ACTGGCTGTA CTTCATTCCC CTCATTATCA TCGGATCCTT CTTTGTCCTT
    AATCTTGTCC TTGGAGTGCT GTCCGGTGAA TTTGCCAAAG AGAGGGAAAG AGTGGAAAAC
    CGCCGGGCTT TCATGAAGTT GCGCAGGCAG CAGCAGATTG AGAGGGAGTT GAATGGCTAT
    CGGGCATGGA TAGATAAAGC TGAGGAAGTC ATGTTGGCAG AAGAAAACAA AAATTCTGGG
    ACCTCTGCTT TAGAAGTTCT TAGGCGAGCA ACCATTAAAC GAAGTAGAAC AGAAGCCATG
    AACCGGGACT CTACTGAGGA CCATTGTGTT GACATATCCT CTGTTGGCAC CCCCCTTGCT
    AGAGCAAGCA TCAAAAGTAC CAAAATGGAT GGGGCATCAT ATTTTCGGCA CAAGGAAAAA
    CTATTGCGTG TCTCCGTTCG TCACATGATC AAATCCCAGG TTTTCTACTG GATGGTCCTG
    GGAGTGGTAG CACTTAACAC TTTCTGTGTG GCTATTGTCC ATCACAACCA GACCCTGTGG
    CTCTCAAATG TTCTTTACTA TGCAGAATTT CTGTTTCTTG GGTTATTTTT GGTGGAGATG
    GCTTTTAAAA TGTATGGAAT GGGTCCACGT CTTTACTTTC ACTCATCCTT CAACTGCTTT
    GATTGCGGGG TCACAATAGG TAGTATATTT GAAGTACTTT GGGCCATATT TAGACCAGGA
    ACTTCCTTTG GAATCAGTGT CTTAAGAGCT CTCCGACTTT TGAGAATCTT CAAAATAACA
    AAATACTGGG CCTCACTACG AAACCTTGTA GTATCATTGA TGAATTCTAT GAAATCCATC
    ATCAGCCTGC TCTTCCTTCT ATTCCTCTTC ATAGTTGTTT TTGCTCTCCT TGGAATGCAG
    TTATATGGTG GCAGATTCAA TTTTAATGAT GGCACTCCTT CTGCAAACTT TGATACATTC
    CCTGCAGCCA TCATGACTGT ATTCCAGATT CTGACAGGAG AGGATTGGAA TGAAGTGATG
    TACAATGGTA TTCGCTCCCA AGGTGGTGTT CGATCCGGAA TGTGGTCTTC CATATACTTT
    ATTGTCCTTA CCCTTTTTGG AAATTACACA CTGCTAAATG TATTTCTTGC CATTGCTGTG
    GACAACCTGG CTAATGCACA GGAGTTAACT AAGGATGAGC AAGAAGAAGA GGAAGCTTTC
    AATCAGAAGC ATGCACTGCA AAAAGCGAAG GAGGTCAGCC CCATGTCTGC ACCTAATGCA
    TCATGTATTG AGAAGGAGCG CAGAAAACGG CACACATTGT CTATATGGGA GCAGCGCACA
    AGCCAGCTAA GGAGGCACAT ACAGATGTCT AGTCAAGAAG CTCTCCAGAG TGAAGAACTG
    GCAGCATTGA ATCCACACTC CAGTATCTAT CGCAAGACAA AGGAAGCTGC TGAACAAAAC
    ACAGAAAAAG AAGAAGTGCA TCAAGACGCA AAACTGGCAA TAGACCCAGT TGAAGGACCA
    CCAGAAGGTG AAGGAAGCCC TTCTAATAGA AATCTCAGGG AAAACTGGCG CCAGAAGTCC
    AGGCATGGAA ATTCTGAAAA CACTGAGCCA AATGGAACAA CTCCAGAAGA AGCAATGCGC
    CGCAGACAAA GTCAGAGACG CAGCCGTCAT AGAAGAGTTA GAACTGAAGG TCGTGAGCAG
    TGTAGCAATC CAAACAGTAC CTCAGCAAGC AGAGAAGGTG GTCTTGACAA AGTCTCTGCA
    GAGGATGGAA GAACAATTCC AGAAGAGAAG AGCAATGGAG AAGCCAAAGA AGTCAAAGGG
    GTTGATGATG AGCCTCTAAA GATTTGTATA TCTTCAAGAA ATTGCGAGGA CAGAAATATC
    AGGACTTTGG GTTGTGAGGG TATTGCCTTG AACGATACAC CCCTGCAGGA TGTGAACAGC
    CAAGAGAAGG ACAGTAGCCC TCCTGATTTG GATTGCAGTG AGCAGGCTCT GTTGGGCAGC
    ATTCAGAGCC AGGAAAGCCC TAATCCAACC AGCTGCAGTT TGCCAGATGT GTCCAGCAGC
    ACCGAGAGAG TCACAGAGAG CACCGTCATT ACCTTACCAG ATACTGGAAT GCCCACCGAC
    TGCACCGTGG TACAGATGAG TGAAGGCAGG GATGGAGAAG TCAACTTTGG GTCTTCGCAG
    CAAGAAAAAG AAGAGGAGGA AGCTGAGGAT GGAGAAGAAA GAACTATAAA AAGAAAAGAG
    AAATTGTTGG AGTCCCCCAA AGCCATTGTA CCGTACAGTT CAATGCTCAT CTTCAGCTCC
    ACCAATCCCA TAAGAAAAGT ATGTCATTAC ATTGTTAACC TACGATACTT TGAGATGAGC
    ATCCTGCTGG TAATAGCAGC AAGTAGTATA GCCTTAGCAG CGGAGGACCC TGTACTGACC
    AACTCGGACC GCAACAAAGT TCTTCGCTAT TTTGACTATG TTTTCACAGG GGTCTTCACT
    TTTGAGATGA TTATAAAGAT GATTGACCAA GGTTTGATTT TACACGAGGG CTCCTACTTT
    CGTGACCTAT GGAACATCTT GGATTTTGTT GTTGTTGTGG GAGCCTTAAT TGCGTTTGCC
    TTAGCGAATG TTTTGGGGAC TAACAAAGGC CGGGACATCA AAACCATTAA GTCTCTACGT
    GTGTTACGGG TGCTGCGTCC ACTAAAAACA ATCAAACGCC TGCCAAAACT GAAGGCAGTC
    TTTGATTGTG TGGTTAACTC TTTAAAGAAT GTCTTTAATA TTCTCATCGT ATACAAACTC
    TTCATGTTCA TATTTGCTGT AATCGCTGTC CAGCTGTTTA AGGGCAAGTT CTTCTATTGT
    ACAGACAGCT CAAAGGAGAC AGAGAAAGAG TGCATAGGGA GTTACATCGA TCACGAAAAG
    AACAAGATGG AGGTGAAGCG GAGAGAGTGG AAAAGACATG ATTTCCATTA TGACAACATA
    ATATGGGCTC TTCTTACCCT CTTTACTGTA TCAACAGGGG AAGGCTGGCC TCAGGTTCTG
    CAGCACTCTG TTGATATAAC TGAAGAAGAT AGGGGTCCAA GCCGAAGCAA CCGTATGGAG
    ATGTCCATTT TCTATGTTAT CTACTTTGTG GTCTTCCCCT TCTTCTTTGT AAATATATTT
    GTGGCTCTGA TTATCATCAC ATTCCAGGAG CAAGGAGACA AGATGATGGA GGAATGTTCC
    CTAGAAAAGA ATGAGAGAGC CTGCATAGAC TTTGCAATAA GTGCTAAGCC ACTTACACGA
    TATATGCCAC AGAACAGGCA CACATTCCAG TACCGGGTCT GGCACTTCGT GGTCTCCCCA
    TCATTTGAAT ACACTATCAT GGCTATGATT GCACTAAACA CAGTGGTACT CATGATGAAG
    TATTATTCTG CGCCATACAC ATATGAGCTT GCATTAAAGT ATCTCAATAT TGCATTCACA
    ATGCTATTTT CTCTGGAGTG CATCCTGAAA ATAATTGCAT TTGGGTTTTT GAATTACTTT
    CGGGATACTT GGAACATTTT TGACTTCATT ACTGTAATTG GTAGCATCAC GGAAATTATT
    CTAACAGATA GTAAGTTGGT GAACACCAGC AGCTTTAATA TGAGCTTTCT GAAGCTGTTC
    CGAGCGGCGC GACTTATTAA ACTTCTCCGG CAAGGGTACA CCATCCGGAT CCTGCTGTGG
    ACTTTTGTTC AGTCCTTTAA GGCACTTCCT TATGTGTGTC TTCTCATTGC AATGTTGTTC
    TTCATCTATG CCATTATTGG GATGCAGGTG TTTGGCAATA TCCGCCTGGA AGAGGACAGT
    CATATTAATA GGCACAACAA TTTTAGAAGC TTCTTTAGTT CTCTTATGTT ACTCTTCAGG
    AGTGCCACAG GAGAAGCGTG GCAGGAGATC ATGCTTTCAT GCCTTAGTGA TAAGGGTTGT
    GAGCCAGACA CCAGTGCAGC CTCTGGACAC AACGAGAATG AGCGCTGTGG AACTGAACTT
    GCATATGTTT ATTTTGTTTC ATTCATTTTC TTCTGCTCCT TCCTCATGCT AAATCTGTTT
    GTGGCTGTCA TTATGGACAA CTTTGAGTAC TTGACACGGG ACTCTTCCAT TTTGGGACCT
    CATCACTTGG ATGAGTTTGT TCGGATATGG GCTGAATATG ATCGAGCAGC ATGTGGCCGT
    ATCCATTACA CAGAGATGTA TGACATGCTG ACTCTCATGT CACCACCTCT AGGCCTTGGA
    AAGAGATGCC CTAACAAAGT GGCTTATAAG AGATTGGTCC TGATGAATAT GCCAGTGTCG
    GAGGACATGA CGGTTCATTT CACATCAACT CTTATGGCCC TAATACGCAC GGCACTGGAT
    ATCAAAATAG CTAAAGGAGG TGCTGACAGG CAACAGCTGG ACTCAGAGCT GCAGAAAGAT
    ATACTTGCCA TTTGGCCAAA TCTCTCCCAG AAAATGATCG ACTTACTTGT GCCAATGCCA
    AGAGCCTCTG ATCTGACGGT GGGGAAGATT TACGCTGCAA TGATGATCAT GGATTACTAT
    AAACAGAGCA AAGCGAAAAA ACAAAGGCAG CAGCTGGAGG AGCAGAAAAA TGCACCTATG
    TTTCAGAGAA TGGAGGCTTC TGCCCTCCCT GAGGAGATCT TATCCAATGC CAAAGCTTTA
    CCATACCTTC AGCAAGACAC TCTATCAGGC CTGGGTGGAT TTCCTTCACT GAGTCCCTTG
    TCCCCAGAAG AAGTATTCCA GCCGCCATGC CTAGACGGGG AAGAGGAGGA AGACTTTGAA
    GAGCAGCTGT CTCTGGTCCT GTCAGACGTA AGCTCCATGC GTCGCTCCTT CTCCACAACT
    GCAGAGAAGC GAGTGCGCAG CTCGTGGCTA GATGAGTTCA CTATGGACAG GAGTAGCACT
    GACTGTGCAT ACAGATACCA GAGGCGCAGT TATCATGCAT CCTTAAGACT GTCTGCACAT
    CGCCTGAATA CAGATACAGG TCACTGGTCA GATAATCACC GTTCTGGCGG AAGGGAGCGA
    GGAAGGTCCA AAGAGAGAAA ACACTTGCTG TCACCCGATC CTTCCCGCTG TAATTCTGAG
    GAAAGGAGCC CACAAGCTGC CTGTGAACAC CGAGATCACC AACTGTCCCC TGGGCATACT
    CCCAGTAGTG TACAGATTCT TGACTCTCTG AGTGAAAGTT GTACCCCTTC AGACACCAGC
    ACCCCAAGAA GAGGCCGTAG ACAGCTTCCT TCTGTGCCTC CAACTCCTAG ACCCCTTATC
    TCTTTCTCCT CGTTAATCCG CCGGCAGAAA CACAGCCCCC CTCCTCAAAA TAAAAATGAT
    ACTATCCTTC CTAGTGAGCC TGGTCTGACT GAGTCTTCCT GCTCCCTTGG GGGTCATCAG
    AGCTCCCAGT CCACACCACA GCATTATATC TCAGAGCCCT ACCTGGCACT GCATGAGGAG
    ATGCAAAGCT CAGACTGCCC AGCAGAGGAC ACACTCACCT TTGAGGTTGC TGTGGCTACT
    AGTCTGGGCC GCTCGAACAC CATTGGCTCT GCCCCTCTAC GTCATGGCTG GCAAATGCCC
    AATGGTCACT ACCGGCGTCG AAGGAAGGGA GGAATTCCAC CAGTGATACC CTCCAGAGAG
    TTAAGTGACA CAGAAGAGGA TGATCGATGT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa57233
    Clone ID Related Accession (Same CDS sequence) XM_031900982.1
    Accession Version XM_031900982.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6783bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGTTAGCGC CGGCAGGAGC CGGGGAGCAG ATCGGCACAG GAGTGCAGCT TCAACCCGGC 
    TATAGGATGA TGGCTCGTTT CGGAGAGGCT GTGACTGGCA GGTTGAGCTC CGCAGATGGG
    GGCTCAGAGA GGAGCAAGAC CCTACAAGGT TCCCCAGCTG CACCTGTTGG GACCCAAGCA
    GCTTTCAAGC AATCCAAAGC CCAGAGGGCA AGGACCATGG CTTTATACAA CCCCATCCCT
    GTCCGACAGA ACTGCTTCAC CGTGAACAGG TCTCTCTTCA TTTTCGGAGA AGATAATATT
    ATCAGGAAAT ACGCCAAGAA GCTCATTGAT TGGCCTCCTT TTGAATACAT GATCCTTGCC
    ACAATTATTG CAAACTGCAT TGTCCTGGCT TTGGAGCAGC ATCTTCCAGG CGATGACAAG
    ACTCCAATGT CTCGCAGATT GGAAAAGACT GAGCCCTATT TCATTGGGAT ATTTTGCTTT
    GAAGCTGGAA TTAAAATTGT CGCTCTGGGA CTGGTTTTTC ACAAAGGTTC TTATCTTCGG
    AATGGCTGGA ATGTCATGGA CTTTATAGTA GTGCTCAGTG GCATCCTGGC CACAGCTGGA
    ACTCACTTCA ATACTCACGT GGACCTGCGT ACTTTGCGGG CAGTGCGTGT TCTCAGGCCG
    TTGAAGCTGG TCTCGGGGAT TCCCAGTTTA CAGATTGTGT TGAAGTCAAT CATGAAGGCG
    ATGGTCCCTC TGCTACAGAT TGGATTGTTG CTCTTCTTTG CCATCCTTAT GTTTGCTATC
    ATCGGCCTTG AGTTTTACAG TGGAAAGCTA CACAAGGCCT GTTACAGGAA CAACTCTGGA
    ATGGAGCTAG ACCCCATGGA TCCACCTCAC CCTTGTGGAG TGCCCCGCTG CCCAGCTGGG
    TATGAGTGCA AGGAATGGAT CGGACCCAAT GATGGAATAA CACAGTTTGA CAACATCCTA
    TTTGCGGTGC TGACAGTCTT CCAGTGTATA ACTATGGAGG GCTGGACCAC AGTGCTGTAC
    AATACCAATG ATGCCTTAGG AGCCACATGG AACTGGCTGT ACTTCATTCC CCTCATTATC
    ATCGGATCCT TCTTTGTCCT TAATCTTGTC CTTGGAGTGC TGTCCGGTGA ATTTGCCAAA
    GAGAGGGAAA GAGTGGAAAA CCGCCGGGCT TTCATGAAGT TGCGCAGGCA GCAGCAGATT
    GAGAGGGAGT TGAATGGCTA TCGGGCATGG ATAGATAAAG CTGAGGAAGT CATGTTGGCA
    GAAGAAAACA AAAATTCTGG GACCTCTGCT TTAGAAGTTC TTAGGCGAGC AACCATTAAA
    CGAAGTAGAA CAGAAGCCAT GAACCGGGAC TCTACTGAGG ACCATTGTGT TGACATATCC
    TCTGTTGGCA CCCCCCTTGC TAGAGCAAGC ATCAAAAGTA CCAAAATGGA TGGGGCATCA
    TATTTTCGGC ACAAGGAAAA ACTATTGCGT GTCTCCGTTC GTCACATGAT CAAATCCCAG
    GTTTTCTACT GGATGGTCCT GGGAGTGGTA GCACTTAACA CTTTCTGTGT GGCTATTGTC
    CATCACAACC AGACCCTGTG GCTCTCAAAT GTTCTTTACT ATGCAGAATT TCTGTTTCTT
    GGGTTATTTT TGGTGGAGAT GGCTTTTAAA ATGTATGGAA TGGGTCCACG TCTTTACTTT
    CACTCATCCT TCAACTGCTT TGATTGCGGG GTCACAATAG GTAGTATATT TGAAGTACTT
    TGGGCCATAT TTAGACCAGG AACTTCCTTT GGAATCAGTG TCTTAAGAGC TCTCCGACTT
    TTGAGAATCT TCAAAATAAC AAAATACTGG GCCTCACTAC GAAACCTTGT AGTATCATTG
    ATGAATTCTA TGAAATCCAT CATCAGCCTG CTCTTCCTTC TATTCCTCTT CATAGTTGTT
    TTTGCTCTCC TTGGAATGCA GTTATATGGT GGCAGATTCA ATTTTAATGA TGGCACTCCT
    TCTGCAAACT TTGATACATT CCCTGCAGCC ATCATGACTG TATTCCAGAT TCTGACAGGA
    GAGGATTGGA ATGAAGTGAT GTACAATGGT ATTCGCTCCC AAGGTGGTGT TCGATCCGGA
    ATGTGGTCTT CCATATACTT TATTGTCCTT ACCCTTTTTG GAAATTACAC ACTGCTAAAT
    GTATTTCTTG CCATTGCTGT GGACAACCTG GCTAATGCAC AGGAGTTAAC TAAGGATGAG
    CAAGAAGAAG AGGAAGCTTT CAATCAGAAG CATGCACTGC AAAAAGCGAA GGAGGTCAGC
    CCCATGTCTG CACCTAATGC ATCATGTATT GAGAAGGAGC GCAGAAAACG GCACACATTG
    TCTATATGGG AGCAGCGCAC AAGCCAGCTA AGGAGGCACA TACAGATGTC TAGTCAAGAA
    GCTCTCCAGA GTGAAGAACT GGCAGCATTG AATCCACACT CCAGTATCTA TCGCAAGACA
    AAGGAAGCTG CTGAACAAAA CACAGAAAAA GAAGAAGTGC ATCAAGACGC AAAACTGGCA
    ATAGACCCAG TTGAAGGACC ACCAGAAGGT GAAGGAAGCC CTTCTAATAG AAATCTCAGG
    GAAAACTGGC GCCAGAAGTC CAGGCATGGA AATTCTGAAA ACACTGAGCC AAATGGAACA
    ACTCCAGAAG AAGCAATGCG CCGCAGACAA AGTCAGAGAC GCAGCCGTCA TAGAAGAGTT
    AGAACTGAAG GTCGTGAGCA GTGTAGCAAT CCAAACAGTA CCTCAGCAAG CAGAGAAGGT
    GGTCTTGACA AAGTCTCTGC AGAGGATGGA AGAACAATTC CAGAAGAGAA GAGCAATGGA
    GAAGCCAAAG AAGTCAAAGG GGTTGATGAT GAGCCTCTAA AGATTTGTAT ATCTTCAAGA
    AATTGCGAGG ACAGAAATAT CAGGACTTTG GGTTGTGAGG GTATTGCCTT GAACGATACA
    CCCCTGCAGG ATGTGAACAG CCAAGAGAAG GACAGTAGCC CTCCTGATTT GGATTGCAGT
    GAGCAGGCTC TGTTGGGCAG CATTCAGAGC CAGGAAAGCC CTAATCCAAC CAGCTGCAGT
    TTGCCAGATG TGTCCAGCAG CACCGAGAGA GTCACAGAGA GCACCGTCAT TACCTTACCA
    GATACTGGAA TGCCCACCGA CTGCACCGTG GTACAGATGA GTGAAGGCAG GGATGGAGAA
    GTCAACTTTG GGTCTTCGCA GCAAGAAAAA GAAGAGGAGG AAGCTGAGGA TGGAGAAGAA
    AGAACTATAA AAAGAAAAGA GAAATTGTTG GAGTCCCCCA AAGCCATTGT ACCGTACAGT
    TCAATGCTCA TCTTCAGCTC CACCAATCCC ATAAGAAAAG TATGTCATTA CATTGTTAAC
    CTACGATACT TTGAGATGAG CATCCTGCTG GTAATAGCAG CAAGTAGTAT AGCCTTAGCA
    GCGGAGGACC CTGTACTGAC CAACTCGGAC CGCAACAAAG TTCTTCGCTA TTTTGACTAT
    GTTTTCACAG GGGTCTTCAC TTTTGAGATG ATTATAAAGA TGATTGACCA AGGTTTGATT
    TTACACGAGG GCTCCTACTT TCGTGACCTA TGGAACATCT TGGATTTTGT TGTTGTTGTG
    GGAGCCTTAA TTGCGTTTGC CTTAGCGAAT GTTTTGGGGA CTAACAAAGG CCGGGACATC
    AAAACCATTA AGTCTCTACG TGTGTTACGG GTGCTGCGTC CACTAAAAAC AATCAAACGC
    CTGCCAAAAC TGAAGGCAGT CTTTGATTGT GTGGTTAACT CTTTAAAGAA TGTCTTTAAT
    ATTCTCATCG TATACAAACT CTTCATGTTC ATATTTGCTG TAATCGCTGT CCAGCTGTTT
    AAGGGCAAGT TCTTCTATTG TACAGACAGC TCAAAGGAGA CAGAGAAAGA GTGCATAGGG
    AGTTACATCG ATCACGAAAA GAACAAGATG GAGGTGAAGC GGAGAGAGTG GAAAAGACAT
    GATTTCCATT ATGACAACAT AATATGGGCT CTTCTTACCC TCTTTACTGT ATCAACAGGG
    GAAGGCTGGC CTCAGGTTCT GCAGCACTCT GTTGATATAA CTGAAGAAGA TAGGGGTCCA
    AGCCGAAGCA ACCGTATGGA GATGTCCATT TTCTATGTTA TCTACTTTGT GGTCTTCCCC
    TTCTTCTTTG TAAATATATT TGTGGCTCTG ATTATCATCA CATTCCAGGA GCAAGGAGAC
    AAGATGATGG AGGAATGTTC CCTAGAAAAG AATGAGAGAG CCTGCATAGA CTTTGCAATA
    AGTGCTAAGC CACTTACACG ATATATGCCA CAGAACAGGC ACACATTCCA GTACCGGGTC
    TGGCACTTCG TGGTCTCCCC ATCATTTGAA TACACTATCA TGGCTATGAT TGCACTAAAC
    ACAGTGGTAC TCATGATGAA GTATTATTCT GCGCCATACA CATATGAGCT TGCATTAAAG
    TATCTCAATA TTGCATTCAC AATGCTATTT TCTCTGGAGT GCATCCTGAA AATAATTGCA
    TTTGGGTTTT TGAATTACTT TCGGGATACT TGGAACATTT TTGACTTCAT TACTGTAATT
    GGTAGCATCA CGGAAATTAT TCTAACAGAT AGTAAGTTGG TGAACACCAG CAGCTTTAAT
    ATGAGCTTTC TGAAGCTGTT CCGAGCGGCG CGACTTATTA AACTTCTCCG GCAAGGGTAC
    ACCATCCGGA TCCTGCTGTG GACTTTTGTT CAGTCCTTTA AGGCACTTCC TTATGTGTGT
    CTTCTCATTG CAATGTTGTT CTTCATCTAT GCCATTATTG GGATGCAGGT GTTTGGCAAT
    ATCCGCCTGG AAGAGGACAG TCATATTAAT AGGCACAACA ATTTTAGAAG CTTCTTTAGT
    TCTCTTATGT TACTCTTCAG GAGTGCCACA GGAGAAGCGT GGCAGGAGAT CATGCTTTCA
    TGCCTTAGTG ATAAGGGTTG TGAGCCAGAC ACCAGTGCAG CCTCTGGACA CAACGAGAAT
    GAGCGCTGTG GAACTGAACT TGCATATGTT TATTTTGTTT CATTCATTTT CTTCTGCTCC
    TTCCTCATGC TAAATCTGTT TGTGGCTGTC ATTATGGACA ACTTTGAGTA CTTGACACGG
    GACTCTTCCA TTTTGGGACC TCATCACTTG GATGAGTTTG TTCGGATATG GGCTGAATAT
    GATCGAGCAG CATGTGGCCG TATCCATTAC ACAGAGATGT ATGACATGCT GACTCTCATG
    TCACCACCTC TAGGCCTTGG AAAGAGATGC CCTAACAAAG TGGCTTATAA GAGATTGGTC
    CTGATGAATA TGCCAGTGTC GGAGGACATG ACGGTTCATT TCACATCAAC TCTTATGGCC
    CTAATACGCA CGGCACTGGA TATCAAAATA GCTAAAGGAG GTGCTGACAG GCAACAGCTG
    GACTCAGAGC TGCAGAAAGA TATACTTGCC ATTTGGCCAA ATCTCTCCCA GAAAATGATC
    GACTTACTTG TGCCAATGCC AAGAGCCTCT GATCTGACGG TGGGGAAGAT TTACGCTGCA
    ATGATGATCA TGGATTACTA TAAACAGAGC AAAGCGAAAA AACAAAGGCA GCAGCTGGAG
    GAGCAGAAAA ATGCACCTAT GTTTCAGAGA ATGGAGGCTT CTGCCCTCCC TGAGGAGATC
    TTATCCAATG CCAAAGCTTT ACCATACCTT CAGCAAGACA CTCTATCAGG CCTGGGTGGA
    TTTCCTTCAC TGAGTCCCTT GTCCCCAGAA GAAGTATTCC AGCCGCCATG CCTAGACGGG
    GAAGAGGAGG AAGACTTTGA AGAGCAGCTG TCTCTGGAGC CTGAGGTTAG AGAGTTTAGA
    AGGGAGCAGC TCCCTAACCG AGGCAGCTAC ATCCCTGTGG AGATGCTGGA ACGTCCAGCG
    TCTGTGAGAG CTTCTTCCAT GCCACGTCTG GCTGAGAATC CTCAGGTCCT GTCAGACGTA
    AGCTCCATGC GTCGCTCCTT CTCCACAACT GCAGAGAAGC GAGTGCGCAG CTCGTGGCTA
    GATGAGTTCA CTATGGACAG GAGTAGCACT GACTGTGCAT ACAGATACCA GAGGCGCAGT
    TATCATGCAT CCTTAAGACT GTCTGCACAT CGCCTGAATA CAGATACAGG TCACTGGTCA
    GATAATCACC GTTCTGGCGG AAGGGAGCGA GGAAGGTCCA AAGAGAGAAA ACACTTGCTG
    TCACCCGATC CTTCCCGCTG TAATTCTGAG GAAAGGAGCC CACAAGCTGC CTGTGAACAC
    CGAGATCACC AACTGTCCCC TGGGCATACT CCCAGTAGTG TACAGATTCT TGACTCTCTG
    AGTGAAAGTT GTACCCCTTC AGACACCAGC ACCCCAAGAA GAGGCCGTAG ACAGCTTCCT
    TCTGTGCCTC CAACTCCTAG ACCCCTTATC TCTTTCTCCT CGTTAATCCG CCGGCAGAAA
    CACAGCCCCC CTCCTCAAAA TAAAAATGAT ACTATCCTTC CTAGTGAGCC TGGTCTGACT
    GAGTCTTCCT GCTCCCTTGG GGGTCATCAG AGCTCCCAGT CCACACCACA GCATTATATC
    TCAGAGCCCT ACCTGGCACT GCATGAGGAG ATGCAAAGCT CAGACTGCCC AGCAGAGGAC
    ACACTCACCT TTGAGGTTGC TGTGGCTACT AGTCTGGGCC GCTCGAACAC CATTGGCTCT
    GCCCCTCTAC GTCATGGCTG GCAAATGCCC AATGGTCACT ACCGGCGTCG AAGGAAGGGA
    GGAATTCCAC CAGTGATACC CTCCAGAGAG TTAAGTGACA CAGAAGAGGA TGATCGATGT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031756842.1
    CDS77..6859
    Translation

    Target ORF information:

    RefSeq Version XM_031900982.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calcium channel, voltage-dependent, R type, alpha 1E subunit (cacna1e), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031900982.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGTTAGCGC CGGCAGGAGC CGGGGAGCAG ATCGGCACAG GAGTGCAGCT TCAACCCGGC 
    TATAGGATGA TGGCTCGTTT CGGAGAGGCT GTGACTGGCA GGTTGAGCTC CGCAGATGGG
    GGCTCAGAGA GGAGCAAGAC CCTACAAGGT TCCCCAGCTG CACCTGTTGG GACCCAAGCA
    GCTTTCAAGC AATCCAAAGC CCAGAGGGCA AGGACCATGG CTTTATACAA CCCCATCCCT
    GTCCGACAGA ACTGCTTCAC CGTGAACAGG TCTCTCTTCA TTTTCGGAGA AGATAATATT
    ATCAGGAAAT ACGCCAAGAA GCTCATTGAT TGGCCTCCTT TTGAATACAT GATCCTTGCC
    ACAATTATTG CAAACTGCAT TGTCCTGGCT TTGGAGCAGC ATCTTCCAGG CGATGACAAG
    ACTCCAATGT CTCGCAGATT GGAAAAGACT GAGCCCTATT TCATTGGGAT ATTTTGCTTT
    GAAGCTGGAA TTAAAATTGT CGCTCTGGGA CTGGTTTTTC ACAAAGGTTC TTATCTTCGG
    AATGGCTGGA ATGTCATGGA CTTTATAGTA GTGCTCAGTG GCATCCTGGC CACAGCTGGA
    ACTCACTTCA ATACTCACGT GGACCTGCGT ACTTTGCGGG CAGTGCGTGT TCTCAGGCCG
    TTGAAGCTGG TCTCGGGGAT TCCCAGTTTA CAGATTGTGT TGAAGTCAAT CATGAAGGCG
    ATGGTCCCTC TGCTACAGAT TGGATTGTTG CTCTTCTTTG CCATCCTTAT GTTTGCTATC
    ATCGGCCTTG AGTTTTACAG TGGAAAGCTA CACAAGGCCT GTTACAGGAA CAACTCTGGA
    ATGGAGCTAG ACCCCATGGA TCCACCTCAC CCTTGTGGAG TGCCCCGCTG CCCAGCTGGG
    TATGAGTGCA AGGAATGGAT CGGACCCAAT GATGGAATAA CACAGTTTGA CAACATCCTA
    TTTGCGGTGC TGACAGTCTT CCAGTGTATA ACTATGGAGG GCTGGACCAC AGTGCTGTAC
    AATACCAATG ATGCCTTAGG AGCCACATGG AACTGGCTGT ACTTCATTCC CCTCATTATC
    ATCGGATCCT TCTTTGTCCT TAATCTTGTC CTTGGAGTGC TGTCCGGTGA ATTTGCCAAA
    GAGAGGGAAA GAGTGGAAAA CCGCCGGGCT TTCATGAAGT TGCGCAGGCA GCAGCAGATT
    GAGAGGGAGT TGAATGGCTA TCGGGCATGG ATAGATAAAG CTGAGGAAGT CATGTTGGCA
    GAAGAAAACA AAAATTCTGG GACCTCTGCT TTAGAAGTTC TTAGGCGAGC AACCATTAAA
    CGAAGTAGAA CAGAAGCCAT GAACCGGGAC TCTACTGAGG ACCATTGTGT TGACATATCC
    TCTGTTGGCA CCCCCCTTGC TAGAGCAAGC ATCAAAAGTA CCAAAATGGA TGGGGCATCA
    TATTTTCGGC ACAAGGAAAA ACTATTGCGT GTCTCCGTTC GTCACATGAT CAAATCCCAG
    GTTTTCTACT GGATGGTCCT GGGAGTGGTA GCACTTAACA CTTTCTGTGT GGCTATTGTC
    CATCACAACC AGACCCTGTG GCTCTCAAAT GTTCTTTACT ATGCAGAATT TCTGTTTCTT
    GGGTTATTTT TGGTGGAGAT GGCTTTTAAA ATGTATGGAA TGGGTCCACG TCTTTACTTT
    CACTCATCCT TCAACTGCTT TGATTGCGGG GTCACAATAG GTAGTATATT TGAAGTACTT
    TGGGCCATAT TTAGACCAGG AACTTCCTTT GGAATCAGTG TCTTAAGAGC TCTCCGACTT
    TTGAGAATCT TCAAAATAAC AAAATACTGG GCCTCACTAC GAAACCTTGT AGTATCATTG
    ATGAATTCTA TGAAATCCAT CATCAGCCTG CTCTTCCTTC TATTCCTCTT CATAGTTGTT
    TTTGCTCTCC TTGGAATGCA GTTATATGGT GGCAGATTCA ATTTTAATGA TGGCACTCCT
    TCTGCAAACT TTGATACATT CCCTGCAGCC ATCATGACTG TATTCCAGAT TCTGACAGGA
    GAGGATTGGA ATGAAGTGAT GTACAATGGT ATTCGCTCCC AAGGTGGTGT TCGATCCGGA
    ATGTGGTCTT CCATATACTT TATTGTCCTT ACCCTTTTTG GAAATTACAC ACTGCTAAAT
    GTATTTCTTG CCATTGCTGT GGACAACCTG GCTAATGCAC AGGAGTTAAC TAAGGATGAG
    CAAGAAGAAG AGGAAGCTTT CAATCAGAAG CATGCACTGC AAAAAGCGAA GGAGGTCAGC
    CCCATGTCTG CACCTAATGC ATCATGTATT GAGAAGGAGC GCAGAAAACG GCACACATTG
    TCTATATGGG AGCAGCGCAC AAGCCAGCTA AGGAGGCACA TACAGATGTC TAGTCAAGAA
    GCTCTCCAGA GTGAAGAACT GGCAGCATTG AATCCACACT CCAGTATCTA TCGCAAGACA
    AAGGAAGCTG CTGAACAAAA CACAGAAAAA GAAGAAGTGC ATCAAGACGC AAAACTGGCA
    ATAGACCCAG TTGAAGGACC ACCAGAAGGT GAAGGAAGCC CTTCTAATAG AAATCTCAGG
    GAAAACTGGC GCCAGAAGTC CAGGCATGGA AATTCTGAAA ACACTGAGCC AAATGGAACA
    ACTCCAGAAG AAGCAATGCG CCGCAGACAA AGTCAGAGAC GCAGCCGTCA TAGAAGAGTT
    AGAACTGAAG GTCGTGAGCA GTGTAGCAAT CCAAACAGTA CCTCAGCAAG CAGAGAAGGT
    GGTCTTGACA AAGTCTCTGC AGAGGATGGA AGAACAATTC CAGAAGAGAA GAGCAATGGA
    GAAGCCAAAG AAGTCAAAGG GGTTGATGAT GAGCCTCTAA AGATTTGTAT ATCTTCAAGA
    AATTGCGAGG ACAGAAATAT CAGGACTTTG GGTTGTGAGG GTATTGCCTT GAACGATACA
    CCCCTGCAGG ATGTGAACAG CCAAGAGAAG GACAGTAGCC CTCCTGATTT GGATTGCAGT
    GAGCAGGCTC TGTTGGGCAG CATTCAGAGC CAGGAAAGCC CTAATCCAAC CAGCTGCAGT
    TTGCCAGATG TGTCCAGCAG CACCGAGAGA GTCACAGAGA GCACCGTCAT TACCTTACCA
    GATACTGGAA TGCCCACCGA CTGCACCGTG GTACAGATGA GTGAAGGCAG GGATGGAGAA
    GTCAACTTTG GGTCTTCGCA GCAAGAAAAA GAAGAGGAGG AAGCTGAGGA TGGAGAAGAA
    AGAACTATAA AAAGAAAAGA GAAATTGTTG GAGTCCCCCA AAGCCATTGT ACCGTACAGT
    TCAATGCTCA TCTTCAGCTC CACCAATCCC ATAAGAAAAG TATGTCATTA CATTGTTAAC
    CTACGATACT TTGAGATGAG CATCCTGCTG GTAATAGCAG CAAGTAGTAT AGCCTTAGCA
    GCGGAGGACC CTGTACTGAC CAACTCGGAC CGCAACAAAG TTCTTCGCTA TTTTGACTAT
    GTTTTCACAG GGGTCTTCAC TTTTGAGATG ATTATAAAGA TGATTGACCA AGGTTTGATT
    TTACACGAGG GCTCCTACTT TCGTGACCTA TGGAACATCT TGGATTTTGT TGTTGTTGTG
    GGAGCCTTAA TTGCGTTTGC CTTAGCGAAT GTTTTGGGGA CTAACAAAGG CCGGGACATC
    AAAACCATTA AGTCTCTACG TGTGTTACGG GTGCTGCGTC CACTAAAAAC AATCAAACGC
    CTGCCAAAAC TGAAGGCAGT CTTTGATTGT GTGGTTAACT CTTTAAAGAA TGTCTTTAAT
    ATTCTCATCG TATACAAACT CTTCATGTTC ATATTTGCTG TAATCGCTGT CCAGCTGTTT
    AAGGGCAAGT TCTTCTATTG TACAGACAGC TCAAAGGAGA CAGAGAAAGA GTGCATAGGG
    AGTTACATCG ATCACGAAAA GAACAAGATG GAGGTGAAGC GGAGAGAGTG GAAAAGACAT
    GATTTCCATT ATGACAACAT AATATGGGCT CTTCTTACCC TCTTTACTGT ATCAACAGGG
    GAAGGCTGGC CTCAGGTTCT GCAGCACTCT GTTGATATAA CTGAAGAAGA TAGGGGTCCA
    AGCCGAAGCA ACCGTATGGA GATGTCCATT TTCTATGTTA TCTACTTTGT GGTCTTCCCC
    TTCTTCTTTG TAAATATATT TGTGGCTCTG ATTATCATCA CATTCCAGGA GCAAGGAGAC
    AAGATGATGG AGGAATGTTC CCTAGAAAAG AATGAGAGAG CCTGCATAGA CTTTGCAATA
    AGTGCTAAGC CACTTACACG ATATATGCCA CAGAACAGGC ACACATTCCA GTACCGGGTC
    TGGCACTTCG TGGTCTCCCC ATCATTTGAA TACACTATCA TGGCTATGAT TGCACTAAAC
    ACAGTGGTAC TCATGATGAA GTATTATTCT GCGCCATACA CATATGAGCT TGCATTAAAG
    TATCTCAATA TTGCATTCAC AATGCTATTT TCTCTGGAGT GCATCCTGAA AATAATTGCA
    TTTGGGTTTT TGAATTACTT TCGGGATACT TGGAACATTT TTGACTTCAT TACTGTAATT
    GGTAGCATCA CGGAAATTAT TCTAACAGAT AGTAAGTTGG TGAACACCAG CAGCTTTAAT
    ATGAGCTTTC TGAAGCTGTT CCGAGCGGCG CGACTTATTA AACTTCTCCG GCAAGGGTAC
    ACCATCCGGA TCCTGCTGTG GACTTTTGTT CAGTCCTTTA AGGCACTTCC TTATGTGTGT
    CTTCTCATTG CAATGTTGTT CTTCATCTAT GCCATTATTG GGATGCAGGT GTTTGGCAAT
    ATCCGCCTGG AAGAGGACAG TCATATTAAT AGGCACAACA ATTTTAGAAG CTTCTTTAGT
    TCTCTTATGT TACTCTTCAG GAGTGCCACA GGAGAAGCGT GGCAGGAGAT CATGCTTTCA
    TGCCTTAGTG ATAAGGGTTG TGAGCCAGAC ACCAGTGCAG CCTCTGGACA CAACGAGAAT
    GAGCGCTGTG GAACTGAACT TGCATATGTT TATTTTGTTT CATTCATTTT CTTCTGCTCC
    TTCCTCATGC TAAATCTGTT TGTGGCTGTC ATTATGGACA ACTTTGAGTA CTTGACACGG
    GACTCTTCCA TTTTGGGACC TCATCACTTG GATGAGTTTG TTCGGATATG GGCTGAATAT
    GATCGAGCAG CATGTGGCCG TATCCATTAC ACAGAGATGT ATGACATGCT GACTCTCATG
    TCACCACCTC TAGGCCTTGG AAAGAGATGC CCTAACAAAG TGGCTTATAA GAGATTGGTC
    CTGATGAATA TGCCAGTGTC GGAGGACATG ACGGTTCATT TCACATCAAC TCTTATGGCC
    CTAATACGCA CGGCACTGGA TATCAAAATA GCTAAAGGAG GTGCTGACAG GCAACAGCTG
    GACTCAGAGC TGCAGAAAGA TATACTTGCC ATTTGGCCAA ATCTCTCCCA GAAAATGATC
    GACTTACTTG TGCCAATGCC AAGAGCCTCT GATCTGACGG TGGGGAAGAT TTACGCTGCA
    ATGATGATCA TGGATTACTA TAAACAGAGC AAAGCGAAAA AACAAAGGCA GCAGCTGGAG
    GAGCAGAAAA ATGCACCTAT GTTTCAGAGA ATGGAGGCTT CTGCCCTCCC TGAGGAGATC
    TTATCCAATG CCAAAGCTTT ACCATACCTT CAGCAAGACA CTCTATCAGG CCTGGGTGGA
    TTTCCTTCAC TGAGTCCCTT GTCCCCAGAA GAAGTATTCC AGCCGCCATG CCTAGACGGG
    GAAGAGGAGG AAGACTTTGA AGAGCAGCTG TCTCTGGAGC CTGAGGTTAG AGAGTTTAGA
    AGGGAGCAGC TCCCTAACCG AGGCAGCTAC ATCCCTGTGG AGATGCTGGA ACGTCCAGCG
    TCTGTGAGAG CTTCTTCCAT GCCACGTCTG GCTGAGAATC CTCAGGTCCT GTCAGACGTA
    AGCTCCATGC GTCGCTCCTT CTCCACAACT GCAGAGAAGC GAGTGCGCAG CTCGTGGCTA
    GATGAGTTCA CTATGGACAG GAGTAGCACT GACTGTGCAT ACAGATACCA GAGGCGCAGT
    TATCATGCAT CCTTAAGACT GTCTGCACAT CGCCTGAATA CAGATACAGG TCACTGGTCA
    GATAATCACC GTTCTGGCGG AAGGGAGCGA GGAAGGTCCA AAGAGAGAAA ACACTTGCTG
    TCACCCGATC CTTCCCGCTG TAATTCTGAG GAAAGGAGCC CACAAGCTGC CTGTGAACAC
    CGAGATCACC AACTGTCCCC TGGGCATACT CCCAGTAGTG TACAGATTCT TGACTCTCTG
    AGTGAAAGTT GTACCCCTTC AGACACCAGC ACCCCAAGAA GAGGCCGTAG ACAGCTTCCT
    TCTGTGCCTC CAACTCCTAG ACCCCTTATC TCTTTCTCCT CGTTAATCCG CCGGCAGAAA
    CACAGCCCCC CTCCTCAAAA TAAAAATGAT ACTATCCTTC CTAGTGAGCC TGGTCTGACT
    GAGTCTTCCT GCTCCCTTGG GGGTCATCAG AGCTCCCAGT CCACACCACA GCATTATATC
    TCAGAGCCCT ACCTGGCACT GCATGAGGAG ATGCAAAGCT CAGACTGCCC AGCAGAGGAC
    ACACTCACCT TTGAGGTTGC TGTGGCTACT AGTCTGGGCC GCTCGAACAC CATTGGCTCT
    GCCCCTCTAC GTCATGGCTG GCAAATGCCC AATGGTCACT ACCGGCGTCG AAGGAAGGGA
    GGAATTCCAC CAGTGATACC CTCCAGAGAG TTAAGTGACA CAGAAGAGGA TGATCGATGT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa57234
    Clone ID Related Accession (Same CDS sequence) XM_031900983.1
    Accession Version XM_031900983.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7053bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGTATTAA AGATGCTGGG ATTAAGCTCC TGTTCAACCA GTGCTCATAC ACTCAATGAG 
    CCTGACCAGT CAAGCACACT AGCCATACCA GCATTGGATG CCGACACTCA GAGATATGTC
    TTGGCCCTTC CAGAAGCAGA GACATGGCGC AAGCATCGAT TGGGCCTGAC ACAGGCTACT
    CACTCTTGCA ATCTTCTAGA ACCTGAGACA CTTTCTGAAA CCCTAGTTTC ACGCACTGCA
    AGTTACGATG CCTTGTATGA CCTGCGAGCA AGAGAAGCGG AGTCTAACTT CCCATTTGAA
    GGCAGCAGCA TGTTTGAGGT GACGTCTGGG ATGGATTCTG AGTTTCCTTT AAGCCCCCAT
    GTTATAAAGC GAAGGAGAGG AGGCCTGATA GAACAAAAGG ATATCATCAA GGCCCACGAA
    GCTCATAAGA TTCAGAGTAC TCCCCAGGCT CGACGGAAGG AATGGGAGAT GGCTCGTTTC
    GGAGAGGCTG TGACTGGCAG GTTGAGCTCC GCAGATGGGG GCTCAGAGAG GAGCAAGACC
    CTACAAGGTT CCCCAGCTGC ACCTGTTGGG ACCCAAGCAG CTTTCAAGCA ATCCAAAGCC
    CAGAGGGCAA GGACCATGGC TTTATACAAC CCCATCCCTG TCCGACAGAA CTGCTTCACC
    GTGAACAGGT CTCTCTTCAT TTTCGGAGAA GATAATATTA TCAGGAAATA CGCCAAGAAG
    CTCATTGATT GGCCTCCTTT TGAATACATG ATCCTTGCCA CAATTATTGC AAACTGCATT
    GTCCTGGCTT TGGAGCAGCA TCTTCCAGGC GATGACAAGA CTCCAATGTC TCGCAGATTG
    GAAAAGACTG AGCCCTATTT CATTGGGATA TTTTGCTTTG AAGCTGGAAT TAAAATTGTC
    GCTCTGGGAC TGGTTTTTCA CAAAGGTTCT TATCTTCGGA ATGGCTGGAA TGTCATGGAC
    TTTATAGTAG TGCTCAGTGG CATCCTGGCC ACAGCTGGAA CTCACTTCAA TACTCACGTG
    GACCTGCGTA CTTTGCGGGC AGTGCGTGTT CTCAGGCCGT TGAAGCTGGT CTCGGGGATT
    CCCAGTTTAC AGATTGTGTT GAAGTCAATC ATGAAGGCGA TGGTCCCTCT GCTACAGATT
    GGATTGTTGC TCTTCTTTGC CATCCTTATG TTTGCTATCA TCGGCCTTGA GTTTTACAGT
    GGAAAGCTAC ACAAGGCCTG TTACAGGAAC AACTCTGGAA TGGAGCTAGA CCCCATGGAT
    CCACCTCACC CTTGTGGAGT GCCCCGCTGC CCAGCTGGGT ATGAGTGCAA GGAATGGATC
    GGACCCAATG ATGGAATAAC ACAGTTTGAC AACATCCTAT TTGCGGTGCT GACAGTCTTC
    CAGTGTATAA CTATGGAGGG CTGGACCACA GTGCTGTACA ATACCAATGA TGCCTTAGGA
    GCCACATGGA ACTGGCTGTA CTTCATTCCC CTCATTATCA TCGGATCCTT CTTTGTCCTT
    AATCTTGTCC TTGGAGTGCT GTCCGGTGAA TTTGCCAAAG AGAGGGAAAG AGTGGAAAAC
    CGCCGGGCTT TCATGAAGTT GCGCAGGCAG CAGCAGATTG AGAGGGAGTT GAATGGCTAT
    CGGGCATGGA TAGATAAAGC TGAGGAAGTC ATGTTGGCAG AAGAAAACAA AAATTCTGGG
    ACCTCTGCTT TAGAAGTTCT TAGGCGAGCA ACCATTAAAC GAAGTAGAAC AGAAGCCATG
    AACCGGGACT CTACTGAGGA CCATTGTGTT GACATATCCT CTGTTGGCAC CCCCCTTGCT
    AGAGCAAGCA TCAAAAGTAC CAAAATGGAT GGGGCATCAT ATTTTCGGCA CAAGGAAAAA
    CTATTGCGTG TCTCCGTTCG TCACATGATC AAATCCCAGG TTTTCTACTG GATGGTCCTG
    GGAGTGGTAG CACTTAACAC TTTCTGTGTG GCTATTGTCC ATCACAACCA GACCCTGTGG
    CTCTCAAATG TTCTTTACTA TGCAGAATTT CTGTTTCTTG GGTTATTTTT GGTGGAGATG
    GCTTTTAAAA TGTATGGAAT GGGTCCACGT CTTTACTTTC ACTCATCCTT CAACTGCTTT
    GATTGCGGGG TCACAATAGG TAGTATATTT GAAGTACTTT GGGCCATATT TAGACCAGGA
    ACTTCCTTTG GAATCAGTGT CTTAAGAGCT CTCCGACTTT TGAGAATCTT CAAAATAACA
    AAATACTGGG CCTCACTACG AAACCTTGTA GTATCATTGA TGAATTCTAT GAAATCCATC
    ATCAGCCTGC TCTTCCTTCT ATTCCTCTTC ATAGTTGTTT TTGCTCTCCT TGGAATGCAG
    TTATATGGTG GCAGATTCAA TTTTAATGAT GGCACTCCTT CTGCAAACTT TGATACATTC
    CCTGCAGCCA TCATGACTGT ATTCCAGATT CTGACAGGAG AGGATTGGAA TGAAGTGATG
    TACAATGGTA TTCGCTCCCA AGGTGGTGTT CGATCCGGAA TGTGGTCTTC CATATACTTT
    ATTGTCCTTA CCCTTTTTGG AAATTACACA CTGCTAAATG TATTTCTTGC CATTGCTGTG
    GACAACCTGG CTAATGCACA GGAGTTAACT AAGGATGAGC AAGAAGAAGA GGAAGCTTTC
    AATCAGAAGC ATGCACTGCA AAAAGCGAAG GAGGTCAGCC CCATGTCTGC ACCTAATGCA
    TCATGTATTG AGAAGGAGCG CAGAAAACGG CACACATTGT CTATATGGGA GCAGCGCACA
    AGCCAGCTAA GGAGGCACAT ACAGATGTCT AGTCAAGAAG CTCTCCAGAG TGAAGAACTG
    GCAGCATTGA ATCCACACTC CAGTATCTAT CGCAAGACAA AGGAAGCTGC TGAACAAAAC
    ACAGAAAAAG AAGAAGTGCA TCAAGACGCA AAACTGGCAA TAGACCCAGT TGAAGGACCA
    CCAGAAGGTG AAGGAAGCCC TTCTAATAGA AATCTCAGGG AAAACTGGCG CCAGAAGTCC
    AGGCATGGAA ATTCTGAAAA CACTGAGCCA AATGGAACAA CTCCAGAAGA AGCAATGCGC
    CGCAGACAAA GTCAGAGACG CAGCCGTCAT AGAAGAGTTA GAACTGAAGG TCGTGAGCAG
    TGTAGCAATC CAAACAGTAC CTCAGCAAGC AGAGAAGGTG GTCTTGACAA AGTCTCTGCA
    GAGGATGGAA GAACAATTCC AGAAGAGAAG AGCAATGGAG AAGCCAAAGA AGTCAAAGGG
    GTTGATGATG AGCCTCTAAA GATTTGTATA TCTTCAAGAA ATTGCGAGGA CAGAAATATC
    AGGACTTTGG GTTGTGAGGG TATTGCCTTG AACGATACAC CCCTGCAGGA TGTGAACAGC
    CAAGAGAAGG ACAGTAGCCC TCCTGATTTG GATTGCAGTG AGCAGGCTCT GTTGGGCAGC
    ATTCAGAGCC AGGAAAGCCC TAATCCAACC AGCTGCAGTT TGCCAGATGT GTCCAGCAGC
    ACCGAGAGAG TCACAGAGAG CACCGTCATT ACCTTACCAG ATACTGGAAT GCCCACCGAC
    TGCACCGTGG TACAGATGAG TGAAGGCAGG GATGGAGAAG TCAACTTTGG GTCTTCGCAG
    CAAGAAAAAG AAGAGGAGGA AGCTGAGGAT GGAGAAGAAA GAACTATAAA AAGAAAAGAG
    AAATTGTTGG AGTCCCCCAA AGCCATTGTA CCGTACAGTT CAATGCTCAT CTTCAGCTCC
    ACCAATCCCA TAAGAAAAGT ATGTCATTAC ATTGTTAACC TACGATACTT TGAGATGAGC
    ATCCTGCTGG TAATAGCAGC AAGTAGTATA GCCTTAGCAG CGGAGGACCC TGTACTGACC
    AACTCGGACC GCAACAAAGT TCTTCGCTAT TTTGACTATG TTTTCACAGG GGTCTTCACT
    TTTGAGATGA TTATAAAGAT GATTGACCAA GGTTTGATTT TACACGAGGG CTCCTACTTT
    CGTGACCTAT GGAACATCTT GGATTTTGTT GTTGTTGTGG GAGCCTTAAT TGCGTTTGCC
    TTAGCGAATG TTTTGGGGAC TAACAAAGGC CGGGACATCA AAACCATTAA GTCTCTACGT
    GTGTTACGGG TGCTGCGTCC ACTAAAAACA ATCAAACGCC TGCCAAAACT GAAGGCAGTC
    TTTGATTGTG TGGTTAACTC TTTAAAGAAT GTCTTTAATA TTCTCATCGT ATACAAACTC
    TTCATGTTCA TATTTGCTGT AATCGCTGTC CAGCTGTTTA AGGGCAAGTT CTTCTATTGT
    ACAGACAGCT CAAAGGAGAC AGAGAAAGAG TGCATAGGGA GTTACATCGA TCACGAAAAG
    AACAAGATGG AGGTGAAGCG GAGAGAGTGG AAAAGACATG ATTTCCATTA TGACAACATA
    ATATGGGCTC TTCTTACCCT CTTTACTGTA TCAACAGGGG AAGGCTGGCC TCAGGTTCTG
    CAGCACTCTG TTGATATAAC TGAAGAAGAT AGGGGTCCAA GCCGAAGCAA CCGTATGGAG
    ATGTCCATTT TCTATGTTAT CTACTTTGTG GTCTTCCCCT TCTTCTTTGT AAATATATTT
    GTGGCTCTGA TTATCATCAC ATTCCAGGAG CAAGGAGACA AGATGATGGA GGAATGTTCC
    CTAGAAAAGA ATGAGAGAGC CTGCATAGAC TTTGCAATAA GTGCTAAGCC ACTTACACGA
    TATATGCCAC AGAACAGGCA CACATTCCAG TACCGGGTCT GGCACTTCGT GGTCTCCCCA
    TCATTTGAAT ACACTATCAT GGCTATGATT GCACTAAACA CAGTGGTACT CATGATGAAG
    TATTATTCTG CGCCATACAC ATATGAGCTT GCATTAAAGT ATCTCAATAT TGCATTCACA
    ATGCTATTTT CTCTGGAGTG CATCCTGAAA ATAATTGCAT TTGGGTTTTT GAATTACTTT
    CGGGATACTT GGAACATTTT TGACTTCATT ACTGTAATTG GTAGCATCAC GGAAATTATT
    CTAACAGATA GTAAGTTGGT GAACACCAGC AGCTTTAATA TGAGCTTTCT GAAGCTGTTC
    CGAGCGGCGC GACTTATTAA ACTTCTCCGG CAAGGGTACA CCATCCGGAT CCTGCTGTGG
    ACTTTTGTTC AGTCCTTTAA GGCACTTCCT TATGTGTGTC TTCTCATTGC AATGTTGTTC
    TTCATCTATG CCATTATTGG GATGCAGGTG TTTGGCAATA TCCGCCTGGA AGAGGACAGT
    CATATTAATA GGCACAACAA TTTTAGAAGC TTCTTTAGTT CTCTTATGTT ACTCTTCAGG
    AGTGCCACAG GAGAAGCGTG GCAGGAGATC ATGCTTTCAT GCCTTAGTGA TAAGGGTTGT
    GAGCCAGACA CCAGTGCAGC CTCTGGACAC AACGAGAATG AGCGCTGTGG AACTGAACTT
    GCATATGTTT ATTTTGTTTC ATTCATTTTC TTCTGCTCCT TCCTCATGCT AAATCTGTTT
    GTGGCTGTCA TTATGGACAA CTTTGAGTAC TTGACACGGG ACTCTTCCAT TTTGGGACCT
    CATCACTTGG ATGAGTTTGT TCGGATATGG GCTGAATATG ATCGAGCAGC ATGTGGCAGG
    ATCCCCTATA AGGATATGTA CAAATTAGTC CGAGTCATCT CGCCTCCGCT CGGCCTAGGA
    GAGAACTGCC CATACAGGGT GGCATGCAAG AGATTGGTCC TGATGAATAT GCCAGTGTCG
    GAGGACATGA CGGTTCATTT CACATCAACT CTTATGGCCC TAATACGCAC GGCACTGGAT
    ATCAAAATAG CTAAAGGAGG TGCTGACAGG CAACAGCTGG ACTCAGAGCT GCAGAAAGAT
    ATACTTGCCA TTTGGCCAAA TCTCTCCCAG AAAATGATCG ACTTACTTGT GCCAATGCCA
    AGAGCCTCTG ATCTGACGGT GGGGAAGATT TACGCTGCAA TGATGATCAT GGATTACTAT
    AAACAGAGCA AAGCGAAAAA ACAAAGGCAG CAGCTGGAGG AGCAGAAAAA TGCACCTATG
    TTTCAGAGAA TGGAGGCTTC TGCCCTCCCT GAGGAGATCT TATCCAATGC CAAAGCTTTA
    CCATACCTTC AGCAAGACAC TCTATCAGGC CTGGGTGGAT TTCCTTCACT GAGTCCCTTG
    TCCCCAGAAG AAGTATTCCA GCCGCCATGC CTAGACGGGG AAGAGGAGGA AGACTTTGAA
    GAGCAGCTGT CTCTGGTCCT GTCAGACGTA AGCTCCATGC GTCGCTCCTT CTCCACAACT
    GCAGAGAAGC GAGTGCGCAG CTCGTGGCTA GATGAGTTCA CTATGGACAG GAGTAGCACT
    GACTGTGCAT ACAGATACCA GAGGCGCAGT TATCATGCAT CCTTAAGACT GTCTGCACAT
    CGCCTGAATA CAGATACAGG TCACTGGTCA GATAATCACC GTTCTGGCGG AAGGGAGCGA
    GGAAGGTCCA AAGAGAGAAA ACACTTGCTG TCACCCGATC CTTCCCGCTG TAATTCTGAG
    GAAAGGAGCC CACAAGCTGC CTGTGAACAC CGAGATCACC AACTGTCCCC TGGGCATACT
    CCCAGTAGTG TACAGATTCT TGACTCTCTG AGTGAAAGTT GTACCCCTTC AGACACCAGC
    ACCCCAAGAA GAGGCCGTAG ACAGCTTCCT TCTGTGCCTC CAACTCCTAG ACCCCTTATC
    TCTTTCTCCT CGTTAATCCG CCGGCAGAAA CACAGCCCCC CTCCTCAAAA TAAAAATGAT
    ACTATCCTTC CTAGTGAGCC TGGTCTGACT GAGTCTTCCT GCTCCCTTGG GGGTCATCAG
    AGCTCCCAGT CCACACCACA GCATTATATC TCAGAGCCCT ACCTGGCACT GCATGAGGAG
    ATGCAAAGCT CAGACTGCCC AGCAGAGGAC ACACTCACCT TTGAGGTTGC TGTGGCTACT
    AGTCTGGGCC GCTCGAACAC CATTGGCTCT GCCCCTCTAC GTCATGGCTG GCAAATGCCC
    AATGGTCACT ACCGGCGTCG AAGGAAGGGA GGAATTCCAC CAGTGATACC CTCCAGAGAG
    TTAAGTGACA CAGAAGAGGA TGATCGATGT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031756843.1
    CDS285..7337
    Translation

    Target ORF information:

    RefSeq Version XM_031900983.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calcium channel, voltage-dependent, R type, alpha 1E subunit (cacna1e), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031900983.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGTATTAA AGATGCTGGG ATTAAGCTCC TGTTCAACCA GTGCTCATAC ACTCAATGAG 
    CCTGACCAGT CAAGCACACT AGCCATACCA GCATTGGATG CCGACACTCA GAGATATGTC
    TTGGCCCTTC CAGAAGCAGA GACATGGCGC AAGCATCGAT TGGGCCTGAC ACAGGCTACT
    CACTCTTGCA ATCTTCTAGA ACCTGAGACA CTTTCTGAAA CCCTAGTTTC ACGCACTGCA
    AGTTACGATG CCTTGTATGA CCTGCGAGCA AGAGAAGCGG AGTCTAACTT CCCATTTGAA
    GGCAGCAGCA TGTTTGAGGT GACGTCTGGG ATGGATTCTG AGTTTCCTTT AAGCCCCCAT
    GTTATAAAGC GAAGGAGAGG AGGCCTGATA GAACAAAAGG ATATCATCAA GGCCCACGAA
    GCTCATAAGA TTCAGAGTAC TCCCCAGGCT CGACGGAAGG AATGGGAGAT GGCTCGTTTC
    GGAGAGGCTG TGACTGGCAG GTTGAGCTCC GCAGATGGGG GCTCAGAGAG GAGCAAGACC
    CTACAAGGTT CCCCAGCTGC ACCTGTTGGG ACCCAAGCAG CTTTCAAGCA ATCCAAAGCC
    CAGAGGGCAA GGACCATGGC TTTATACAAC CCCATCCCTG TCCGACAGAA CTGCTTCACC
    GTGAACAGGT CTCTCTTCAT TTTCGGAGAA GATAATATTA TCAGGAAATA CGCCAAGAAG
    CTCATTGATT GGCCTCCTTT TGAATACATG ATCCTTGCCA CAATTATTGC AAACTGCATT
    GTCCTGGCTT TGGAGCAGCA TCTTCCAGGC GATGACAAGA CTCCAATGTC TCGCAGATTG
    GAAAAGACTG AGCCCTATTT CATTGGGATA TTTTGCTTTG AAGCTGGAAT TAAAATTGTC
    GCTCTGGGAC TGGTTTTTCA CAAAGGTTCT TATCTTCGGA ATGGCTGGAA TGTCATGGAC
    TTTATAGTAG TGCTCAGTGG CATCCTGGCC ACAGCTGGAA CTCACTTCAA TACTCACGTG
    GACCTGCGTA CTTTGCGGGC AGTGCGTGTT CTCAGGCCGT TGAAGCTGGT CTCGGGGATT
    CCCAGTTTAC AGATTGTGTT GAAGTCAATC ATGAAGGCGA TGGTCCCTCT GCTACAGATT
    GGATTGTTGC TCTTCTTTGC CATCCTTATG TTTGCTATCA TCGGCCTTGA GTTTTACAGT
    GGAAAGCTAC ACAAGGCCTG TTACAGGAAC AACTCTGGAA TGGAGCTAGA CCCCATGGAT
    CCACCTCACC CTTGTGGAGT GCCCCGCTGC CCAGCTGGGT ATGAGTGCAA GGAATGGATC
    GGACCCAATG ATGGAATAAC ACAGTTTGAC AACATCCTAT TTGCGGTGCT GACAGTCTTC
    CAGTGTATAA CTATGGAGGG CTGGACCACA GTGCTGTACA ATACCAATGA TGCCTTAGGA
    GCCACATGGA ACTGGCTGTA CTTCATTCCC CTCATTATCA TCGGATCCTT CTTTGTCCTT
    AATCTTGTCC TTGGAGTGCT GTCCGGTGAA TTTGCCAAAG AGAGGGAAAG AGTGGAAAAC
    CGCCGGGCTT TCATGAAGTT GCGCAGGCAG CAGCAGATTG AGAGGGAGTT GAATGGCTAT
    CGGGCATGGA TAGATAAAGC TGAGGAAGTC ATGTTGGCAG AAGAAAACAA AAATTCTGGG
    ACCTCTGCTT TAGAAGTTCT TAGGCGAGCA ACCATTAAAC GAAGTAGAAC AGAAGCCATG
    AACCGGGACT CTACTGAGGA CCATTGTGTT GACATATCCT CTGTTGGCAC CCCCCTTGCT
    AGAGCAAGCA TCAAAAGTAC CAAAATGGAT GGGGCATCAT ATTTTCGGCA CAAGGAAAAA
    CTATTGCGTG TCTCCGTTCG TCACATGATC AAATCCCAGG TTTTCTACTG GATGGTCCTG
    GGAGTGGTAG CACTTAACAC TTTCTGTGTG GCTATTGTCC ATCACAACCA GACCCTGTGG
    CTCTCAAATG TTCTTTACTA TGCAGAATTT CTGTTTCTTG GGTTATTTTT GGTGGAGATG
    GCTTTTAAAA TGTATGGAAT GGGTCCACGT CTTTACTTTC ACTCATCCTT CAACTGCTTT
    GATTGCGGGG TCACAATAGG TAGTATATTT GAAGTACTTT GGGCCATATT TAGACCAGGA
    ACTTCCTTTG GAATCAGTGT CTTAAGAGCT CTCCGACTTT TGAGAATCTT CAAAATAACA
    AAATACTGGG CCTCACTACG AAACCTTGTA GTATCATTGA TGAATTCTAT GAAATCCATC
    ATCAGCCTGC TCTTCCTTCT ATTCCTCTTC ATAGTTGTTT TTGCTCTCCT TGGAATGCAG
    TTATATGGTG GCAGATTCAA TTTTAATGAT GGCACTCCTT CTGCAAACTT TGATACATTC
    CCTGCAGCCA TCATGACTGT ATTCCAGATT CTGACAGGAG AGGATTGGAA TGAAGTGATG
    TACAATGGTA TTCGCTCCCA AGGTGGTGTT CGATCCGGAA TGTGGTCTTC CATATACTTT
    ATTGTCCTTA CCCTTTTTGG AAATTACACA CTGCTAAATG TATTTCTTGC CATTGCTGTG
    GACAACCTGG CTAATGCACA GGAGTTAACT AAGGATGAGC AAGAAGAAGA GGAAGCTTTC
    AATCAGAAGC ATGCACTGCA AAAAGCGAAG GAGGTCAGCC CCATGTCTGC ACCTAATGCA
    TCATGTATTG AGAAGGAGCG CAGAAAACGG CACACATTGT CTATATGGGA GCAGCGCACA
    AGCCAGCTAA GGAGGCACAT ACAGATGTCT AGTCAAGAAG CTCTCCAGAG TGAAGAACTG
    GCAGCATTGA ATCCACACTC CAGTATCTAT CGCAAGACAA AGGAAGCTGC TGAACAAAAC
    ACAGAAAAAG AAGAAGTGCA TCAAGACGCA AAACTGGCAA TAGACCCAGT TGAAGGACCA
    CCAGAAGGTG AAGGAAGCCC TTCTAATAGA AATCTCAGGG AAAACTGGCG CCAGAAGTCC
    AGGCATGGAA ATTCTGAAAA CACTGAGCCA AATGGAACAA CTCCAGAAGA AGCAATGCGC
    CGCAGACAAA GTCAGAGACG CAGCCGTCAT AGAAGAGTTA GAACTGAAGG TCGTGAGCAG
    TGTAGCAATC CAAACAGTAC CTCAGCAAGC AGAGAAGGTG GTCTTGACAA AGTCTCTGCA
    GAGGATGGAA GAACAATTCC AGAAGAGAAG AGCAATGGAG AAGCCAAAGA AGTCAAAGGG
    GTTGATGATG AGCCTCTAAA GATTTGTATA TCTTCAAGAA ATTGCGAGGA CAGAAATATC
    AGGACTTTGG GTTGTGAGGG TATTGCCTTG AACGATACAC CCCTGCAGGA TGTGAACAGC
    CAAGAGAAGG ACAGTAGCCC TCCTGATTTG GATTGCAGTG AGCAGGCTCT GTTGGGCAGC
    ATTCAGAGCC AGGAAAGCCC TAATCCAACC AGCTGCAGTT TGCCAGATGT GTCCAGCAGC
    ACCGAGAGAG TCACAGAGAG CACCGTCATT ACCTTACCAG ATACTGGAAT GCCCACCGAC
    TGCACCGTGG TACAGATGAG TGAAGGCAGG GATGGAGAAG TCAACTTTGG GTCTTCGCAG
    CAAGAAAAAG AAGAGGAGGA AGCTGAGGAT GGAGAAGAAA GAACTATAAA AAGAAAAGAG
    AAATTGTTGG AGTCCCCCAA AGCCATTGTA CCGTACAGTT CAATGCTCAT CTTCAGCTCC
    ACCAATCCCA TAAGAAAAGT ATGTCATTAC ATTGTTAACC TACGATACTT TGAGATGAGC
    ATCCTGCTGG TAATAGCAGC AAGTAGTATA GCCTTAGCAG CGGAGGACCC TGTACTGACC
    AACTCGGACC GCAACAAAGT TCTTCGCTAT TTTGACTATG TTTTCACAGG GGTCTTCACT
    TTTGAGATGA TTATAAAGAT GATTGACCAA GGTTTGATTT TACACGAGGG CTCCTACTTT
    CGTGACCTAT GGAACATCTT GGATTTTGTT GTTGTTGTGG GAGCCTTAAT TGCGTTTGCC
    TTAGCGAATG TTTTGGGGAC TAACAAAGGC CGGGACATCA AAACCATTAA GTCTCTACGT
    GTGTTACGGG TGCTGCGTCC ACTAAAAACA ATCAAACGCC TGCCAAAACT GAAGGCAGTC
    TTTGATTGTG TGGTTAACTC TTTAAAGAAT GTCTTTAATA TTCTCATCGT ATACAAACTC
    TTCATGTTCA TATTTGCTGT AATCGCTGTC CAGCTGTTTA AGGGCAAGTT CTTCTATTGT
    ACAGACAGCT CAAAGGAGAC AGAGAAAGAG TGCATAGGGA GTTACATCGA TCACGAAAAG
    AACAAGATGG AGGTGAAGCG GAGAGAGTGG AAAAGACATG ATTTCCATTA TGACAACATA
    ATATGGGCTC TTCTTACCCT CTTTACTGTA TCAACAGGGG AAGGCTGGCC TCAGGTTCTG
    CAGCACTCTG TTGATATAAC TGAAGAAGAT AGGGGTCCAA GCCGAAGCAA CCGTATGGAG
    ATGTCCATTT TCTATGTTAT CTACTTTGTG GTCTTCCCCT TCTTCTTTGT AAATATATTT
    GTGGCTCTGA TTATCATCAC ATTCCAGGAG CAAGGAGACA AGATGATGGA GGAATGTTCC
    CTAGAAAAGA ATGAGAGAGC CTGCATAGAC TTTGCAATAA GTGCTAAGCC ACTTACACGA
    TATATGCCAC AGAACAGGCA CACATTCCAG TACCGGGTCT GGCACTTCGT GGTCTCCCCA
    TCATTTGAAT ACACTATCAT GGCTATGATT GCACTAAACA CAGTGGTACT CATGATGAAG
    TATTATTCTG CGCCATACAC ATATGAGCTT GCATTAAAGT ATCTCAATAT TGCATTCACA
    ATGCTATTTT CTCTGGAGTG CATCCTGAAA ATAATTGCAT TTGGGTTTTT GAATTACTTT
    CGGGATACTT GGAACATTTT TGACTTCATT ACTGTAATTG GTAGCATCAC GGAAATTATT
    CTAACAGATA GTAAGTTGGT GAACACCAGC AGCTTTAATA TGAGCTTTCT GAAGCTGTTC
    CGAGCGGCGC GACTTATTAA ACTTCTCCGG CAAGGGTACA CCATCCGGAT CCTGCTGTGG
    ACTTTTGTTC AGTCCTTTAA GGCACTTCCT TATGTGTGTC TTCTCATTGC AATGTTGTTC
    TTCATCTATG CCATTATTGG GATGCAGGTG TTTGGCAATA TCCGCCTGGA AGAGGACAGT
    CATATTAATA GGCACAACAA TTTTAGAAGC TTCTTTAGTT CTCTTATGTT ACTCTTCAGG
    AGTGCCACAG GAGAAGCGTG GCAGGAGATC ATGCTTTCAT GCCTTAGTGA TAAGGGTTGT
    GAGCCAGACA CCAGTGCAGC CTCTGGACAC AACGAGAATG AGCGCTGTGG AACTGAACTT
    GCATATGTTT ATTTTGTTTC ATTCATTTTC TTCTGCTCCT TCCTCATGCT AAATCTGTTT
    GTGGCTGTCA TTATGGACAA CTTTGAGTAC TTGACACGGG ACTCTTCCAT TTTGGGACCT
    CATCACTTGG ATGAGTTTGT TCGGATATGG GCTGAATATG ATCGAGCAGC ATGTGGCAGG
    ATCCCCTATA AGGATATGTA CAAATTAGTC CGAGTCATCT CGCCTCCGCT CGGCCTAGGA
    GAGAACTGCC CATACAGGGT GGCATGCAAG AGATTGGTCC TGATGAATAT GCCAGTGTCG
    GAGGACATGA CGGTTCATTT CACATCAACT CTTATGGCCC TAATACGCAC GGCACTGGAT
    ATCAAAATAG CTAAAGGAGG TGCTGACAGG CAACAGCTGG ACTCAGAGCT GCAGAAAGAT
    ATACTTGCCA TTTGGCCAAA TCTCTCCCAG AAAATGATCG ACTTACTTGT GCCAATGCCA
    AGAGCCTCTG ATCTGACGGT GGGGAAGATT TACGCTGCAA TGATGATCAT GGATTACTAT
    AAACAGAGCA AAGCGAAAAA ACAAAGGCAG CAGCTGGAGG AGCAGAAAAA TGCACCTATG
    TTTCAGAGAA TGGAGGCTTC TGCCCTCCCT GAGGAGATCT TATCCAATGC CAAAGCTTTA
    CCATACCTTC AGCAAGACAC TCTATCAGGC CTGGGTGGAT TTCCTTCACT GAGTCCCTTG
    TCCCCAGAAG AAGTATTCCA GCCGCCATGC CTAGACGGGG AAGAGGAGGA AGACTTTGAA
    GAGCAGCTGT CTCTGGTCCT GTCAGACGTA AGCTCCATGC GTCGCTCCTT CTCCACAACT
    GCAGAGAAGC GAGTGCGCAG CTCGTGGCTA GATGAGTTCA CTATGGACAG GAGTAGCACT
    GACTGTGCAT ACAGATACCA GAGGCGCAGT TATCATGCAT CCTTAAGACT GTCTGCACAT
    CGCCTGAATA CAGATACAGG TCACTGGTCA GATAATCACC GTTCTGGCGG AAGGGAGCGA
    GGAAGGTCCA AAGAGAGAAA ACACTTGCTG TCACCCGATC CTTCCCGCTG TAATTCTGAG
    GAAAGGAGCC CACAAGCTGC CTGTGAACAC CGAGATCACC AACTGTCCCC TGGGCATACT
    CCCAGTAGTG TACAGATTCT TGACTCTCTG AGTGAAAGTT GTACCCCTTC AGACACCAGC
    ACCCCAAGAA GAGGCCGTAG ACAGCTTCCT TCTGTGCCTC CAACTCCTAG ACCCCTTATC
    TCTTTCTCCT CGTTAATCCG CCGGCAGAAA CACAGCCCCC CTCCTCAAAA TAAAAATGAT
    ACTATCCTTC CTAGTGAGCC TGGTCTGACT GAGTCTTCCT GCTCCCTTGG GGGTCATCAG
    AGCTCCCAGT CCACACCACA GCATTATATC TCAGAGCCCT ACCTGGCACT GCATGAGGAG
    ATGCAAAGCT CAGACTGCCC AGCAGAGGAC ACACTCACCT TTGAGGTTGC TGTGGCTACT
    AGTCTGGGCC GCTCGAACAC CATTGGCTCT GCCCCTCTAC GTCATGGCTG GCAAATGCCC
    AATGGTCACT ACCGGCGTCG AAGGAAGGGA GGAATTCCAC CAGTGATACC CTCCAGAGAG
    TTAAGTGACA CAGAAGAGGA TGATCGATGT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.