LOC100488346 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following LOC100488346 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100488346 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa03801 XM_012970635.3
Latest version!
Xenopus tropicalis plasma membrane calcium-transporting ATPase 4 (LOC100488346), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa03801 XM_004910677.4
Latest version!
Xenopus tropicalis plasma membrane calcium-transporting ATPase 4 (LOC100488346), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa03801 XM_012970637.3
Latest version!
Xenopus tropicalis plasma membrane calcium-transporting ATPase 4 (LOC100488346), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa03805 XM_002932973.5
Latest version!
Xenopus tropicalis plasma membrane calcium-transporting ATPase 4 (LOC100488346), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa42725 XM_018091531.2
Latest version!
Xenopus tropicalis plasma membrane calcium-transporting ATPase 4 (LOC100488346), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa42726 XM_018091532.2
Latest version!
Xenopus tropicalis plasma membrane calcium-transporting ATPase 4 (LOC100488346), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa52243 XM_031896858.1
Latest version!
Xenopus tropicalis plasma membrane calcium-transporting ATPase 4 (LOC100488346), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
View Old Accession Versions >>
OXa42726 XM_018091532.1 Xenopus tropicalis plasma membrane calcium-transporting ATPase 4 (LOC100488346), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OXa03801 XM_012970637.2 Xenopus tropicalis plasma membrane calcium-transporting ATPase 4 (LOC100488346), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa03805 XM_002932973.4 Xenopus tropicalis plasma membrane calcium-transporting ATPase 4 (LOC100488346), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa03801 XM_004910677.3 Xenopus tropicalis plasma membrane calcium-transporting ATPase 4 (LOC100488346), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa03801 XM_012970635.2 Xenopus tropicalis plasma membrane calcium-transporting ATPase 4 (LOC100488346), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa42725 XM_018091531.1 Xenopus tropicalis plasma membrane calcium-transporting ATPase 4 (LOC100488346), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa03801
    Clone ID Related Accession (Same CDS sequence) XM_004910677.3 , XM_012970635.2 , XM_012970637.2 , XM_012970637.3 , XM_004910677.4 , XM_012970635.3
    Accession Version XM_012970637.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3618bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product plasma membrane calcium-transporting ATPase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970637.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATA ACTCCACAGA CCATCATTCT GCTAACATTG TAAATGAAGG CAGCTATGAA 
    GGAGATTTTG GCTGCACAAT AATGGACTTA CGGGAGCTTA TGGAATTACG CAGTGCAGAG
    GCAGTCAACC GTATCAGGGA TACTTATGGA GGGGTTCACA ACATCTGCCG CAGGCTAAAG
    ACTTCACCTG TAGAAGGTTT GTCAGGTAAC CCCTCAGACC TCGAGAGGAG GAGACAAATA
    TTTGGTAAAA ACTTCATCCC TCCAAAAAAA GCCAAAACTT TCTTGCAGCT GGTGTGGGAA
    GCACTGCAGG ACGTTACCTT AATTATCCTG GAAATAGCAG CCATAATATC TCTGGGTCTT
    TCTTTCTACC ACCCACCAGG AGGAGATAAT GAATTATGTG GGGAAGCTGC TGCAGGAGTG
    GAGGATGAAG GTGAGGCTCA AGCTGGATGG ATTGAAGGGG CAGCCATCTT ATTCTCGGTC
    ATCATAGTTG TGCTTGTTAC CGCCTTCAAT GACTGGAGCA AAGAGAAGCA ATTTCGAGGC
    CTACAGAGCC GAATTGAGCA GGAGCAGAAA TTCACTGTTA TCCGCAAAGG ACAGGTTATC
    CAAATCCCTG TAGCAGAGCT AGTAGTCGGT GATATTGCAC AAATAAAATA TGGTGACCTC
    CTTCCTGCTG ATGGGATATT GATTCAAGGC AATGACTTAA AAATTGATGA AAGCTCTCTA
    ACTGGGGAGA GCGATCAGGT GAAAAAATCA TTAGAGAAAG ACCCCATGCT TTTGTCAGGA
    ACACACGTAA TGGAAGGGTC TGGACGTATG GTAGTCACAG CAGTAGGTGT CAACTCTCAA
    ACTGGCATAA TATTCACTTT GCTAGGTGCA AATGAGGGAG GTGAAGAGGA AAAAAAATCC
    AAAAAAGGTA AAAAACCAGG ACCCTCAGAA AATCGCAACA AAGCCAAAGC TCAAGATGGT
    GTTGCATTAG AGATCCAACC ACTGAAAAGT GAAGAGGGCG TAGAGTGTGA AGAGAAAGAG
    AAAAAGGTTG TGAAAGTGCC AAAGAAAGAA AAGTCTGTCC TTCAGGGGAA GCTAACTCGG
    CTGGCAGTGC AAATTGGAAA AGCAGGTCTC ATTATGTCAG CCATCACAGT GATCATTTTG
    GTGCTCTATT TTGTCATTTA TACATTTGGA GTGTTAGGTC GCCCATGGTT GGCAGAATGC
    ACTCCCATCT ACATCCAGTA CTTTGTCAAA TTTTTCATTA TTGGTGTCAC TGTGTTGGTG
    GTGGCTGTCC CTGAGGGGCT GCCTCTCGCT GTAACAATAT CTTTGGCCTA CTCTGTAAAG
    AAAATGATGA AAGACAATAA TCTTGTACGT CACTTGGATG CCTGTGAAAC TATGGGCAAT
    GCAACAGCCA TATGCTCTGA TAAAACTGGA ACGCTCACTA TGAACCGAAT GACTGTAGTA
    CAGGCATTTG TTGGTGGTAC TCATTATAGG CAGATTCCTG ATCCGGAGGC ACTGAACACA
    AAAATTCTTG ATCTTATAGT AAATGGGATT TCAGTCAACT CAGCATACAC CTCCAAGATC
    CTGCCCCCTG AAAAGGAGGG AGGTCTCCCC AGGCAGGTGG GTAACAAGAC AGAATGTGCG
    CTACTGGGAT TTGTTTTGGA CCTGAAGCAA GATTATCAGA CAGTGCGAAA CGAAATTCCT
    GAAGAGAACC TCTACAAAGT TTATACGTTC AATTCTGTGC GAAAATCTAT GAGCACAGTG
    TTGTGTGACT CTTCTGGAAA ATTCCGCATG TACAGCAAAG GGGCCTCTGA AATCATCTTG
    CGCAAATGCA CCCGAATCCT GGACCAAGGA GGAGATGTGT GCCCCTTTAA AGCAAAGGAC
    CGGGATGAGA TGGTAAAGAA AGTAATTGAA CCTATGGCAT GCGATGGCTT GCGCACAATC
    TGCCTAGCAT ATCGTGACTT CCCTTCAGAT AGTGAGCCTA ATTGGGATAA TGAGGGCGAC
    ATCCTCTCTG ACCTTATTTG CATTGCTGTA GTAGGAATAG AAGATCCTGT TCGACCAGAG
    GTGCCTGAAG CAATTCAAAA GTGCCAGCGT GCAGGTATCA CAGTGCGCAT GGTTACTGGG
    GACAATATAA ACACAGCTCG AGCAATAGCT ACCAAGTGTG GTATCCTGCA GCCAGGAGAA
    GACTTCTTGT GCTTGGAGGG AAAAGAATTC AACACACTGA TCCGAAATGA AAAGGGAGAG
    GTAGAACAGG ATAAATTGGA CAAGGTTTGG CCGAGACTTC GTGTTTTGGC CCGGTCATCA
    CCTACAGATA AACATACTCT GGTAAAAGGC ATCATTGACA GCACAGTGGC AGAGCGAAGA
    CAGGTGGTGG CTGTGACTGG AGATGGAACA AACGATGGAC CTGCCCTGAA GAAAGCAGAT
    GTGGGATTTG CAATGGGCAT AGCTGGCACA GATGTTGCAA AGGAGGCTTC TGATATTATC
    TTGACAGACG ACAATTTCAC CAGCATTGTA AAGGCTGTAA TGTGGGGCCG CAATGTATAC
    GACAGTATTT CGAAGTTCCT GCAGTTCCAG CTCACCGTAA ATGTTGTGGC AGTAATTGTG
    GCATTTACAG GAGCCTGCAT AACTCAGGAT TCACCACTGA AAGCTGTGCA GATGCTGTGG
    GTCAACCTTA TCATGGATAC ATTTGCATCT TTAGCTTTGG CGACTGAGCC TCCTACTGAT
    TCGCTTCTTC TACGCAAACC ATATGGGCGC AACAAACCCC TGATCTCACG TACAATGATG
    AAAAACATAT TGGGACATGC TGTTTATCAG CTTACCATCA TATTTACATT GCTTTTTGCA
    GGTGAGAAGT TTTTTGACAT TGACAGTGGG AGAAATGTAC CCCTGCATTC TCCACCTTCT
    GAACATTATA CAATTGTCTT CAACACCTTT GTGATGATGC AGCTTTTTAA TGAGATCAAT
    GCCCGCAAGA TCCACGGTGA ACGTAATGTC TTTGAGAACA TCTTCCGGAA CCCCATCTTT
    TGTGCAGTTG TGCTGGGCAC TTTTGGAGCT CAGATAATAA TTGTGGAGTT TGGAGGAAAG
    CCATTCAGTT GCTCTGGCCT TACTTTGTCA CAGTGGTTCT GGTGTATCTT CATTGGGGTT
    GGAGAGCTGT TGTGGGGACA GATGATTTGC ACAATACCCA CCAGCCGACT CAAGTTCCTA
    AAAGAGGCTG GCCATGGGCC ACTGAAGGAG GACATTCAGC AGGAGGAACT TACAGAGGAT
    GTAGATGAGA TTGACCATGC AGAAATGGAG CTTAGGAGAG GCCAGATTTT GTGGTTCAGA
    GGCCTAAACC GAATACAGAC TCAGATTAAA GTTGTGAATG CGTTCCGAAG CTCTCTCTAC
    GAGGGGCTAG AGAAGCCACA GATCCGCAGT GCCATCCATA GCTTCATGAC CCACCCAGAG
    TTCACTCCAC GGGAGGCAGG ACCTGCCCCC CAATTCGAAG ACAGCGACAG CGAGGGTGAG
    GAATCTAAGG GTCTCTCAAA GGCGCATAAA GATCAAACCC ATAATCACAA CAACAATTCT
    TCTGTTGACC CACATGCATT GGATATCACA GTGTCTCTAT CTGATAGCCC ATTGCATAGC
    ATGGAAACCT CCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012826091.1
    CDS539..4156
    Translation

    Target ORF information:

    RefSeq Version XM_012970637.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis plasma membrane calcium-transporting ATPase 4 (LOC100488346), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970637.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATA ACTCCACAGA CCATCATTCT GCTAACATTG TAAATGAAGG CAGCTATGAA 
    GGAGATTTTG GCTGCACAAT AATGGACTTA CGGGAGCTTA TGGAATTACG CAGTGCAGAG
    GCAGTCAACC GTATCAGGGA TACTTATGGA GGGGTTCACA ACATCTGCCG CAGGCTAAAG
    ACTTCACCTG TAGAAGGTTT GTCAGGTAAC CCCTCAGACC TCGAGAGGAG GAGACAAATA
    TTTGGTAAAA ACTTCATCCC TCCAAAAAAA GCCAAAACTT TCTTGCAGCT GGTGTGGGAA
    GCACTGCAGG ACGTTACCTT AATTATCCTG GAAATAGCAG CCATAATATC TCTGGGTCTT
    TCTTTCTACC ACCCACCAGG AGGAGATAAT GAATTATGTG GGGAAGCTGC TGCAGGAGTG
    GAGGATGAAG GTGAGGCTCA AGCTGGATGG ATTGAAGGGG CAGCCATCTT ATTCTCGGTC
    ATCATAGTTG TGCTTGTTAC CGCCTTCAAT GACTGGAGCA AAGAGAAGCA ATTTCGAGGC
    CTACAGAGCC GAATTGAGCA GGAGCAGAAA TTCACTGTTA TCCGCAAAGG ACAGGTTATC
    CAAATCCCTG TAGCAGAGCT AGTAGTCGGT GATATTGCAC AAATAAAATA TGGTGACCTC
    CTTCCTGCTG ATGGGATATT GATTCAAGGC AATGACTTAA AAATTGATGA AAGCTCTCTA
    ACTGGGGAGA GCGATCAGGT GAAAAAATCA TTAGAGAAAG ACCCCATGCT TTTGTCAGGA
    ACACACGTAA TGGAAGGGTC TGGACGTATG GTAGTCACAG CAGTAGGTGT CAACTCTCAA
    ACTGGCATAA TATTCACTTT GCTAGGTGCA AATGAGGGAG GTGAAGAGGA AAAAAAATCC
    AAAAAAGGTA AAAAACCAGG ACCCTCAGAA AATCGCAACA AAGCCAAAGC TCAAGATGGT
    GTTGCATTAG AGATCCAACC ACTGAAAAGT GAAGAGGGCG TAGAGTGTGA AGAGAAAGAG
    AAAAAGGTTG TGAAAGTGCC AAAGAAAGAA AAGTCTGTCC TTCAGGGGAA GCTAACTCGG
    CTGGCAGTGC AAATTGGAAA AGCAGGTCTC ATTATGTCAG CCATCACAGT GATCATTTTG
    GTGCTCTATT TTGTCATTTA TACATTTGGA GTGTTAGGTC GCCCATGGTT GGCAGAATGC
    ACTCCCATCT ACATCCAGTA CTTTGTCAAA TTTTTCATTA TTGGTGTCAC TGTGTTGGTG
    GTGGCTGTCC CTGAGGGGCT GCCTCTCGCT GTAACAATAT CTTTGGCCTA CTCTGTAAAG
    AAAATGATGA AAGACAATAA TCTTGTACGT CACTTGGATG CCTGTGAAAC TATGGGCAAT
    GCAACAGCCA TATGCTCTGA TAAAACTGGA ACGCTCACTA TGAACCGAAT GACTGTAGTA
    CAGGCATTTG TTGGTGGTAC TCATTATAGG CAGATTCCTG ATCCGGAGGC ACTGAACACA
    AAAATTCTTG ATCTTATAGT AAATGGGATT TCAGTCAACT CAGCATACAC CTCCAAGATC
    CTGCCCCCTG AAAAGGAGGG AGGTCTCCCC AGGCAGGTGG GTAACAAGAC AGAATGTGCG
    CTACTGGGAT TTGTTTTGGA CCTGAAGCAA GATTATCAGA CAGTGCGAAA CGAAATTCCT
    GAAGAGAACC TCTACAAAGT TTATACGTTC AATTCTGTGC GAAAATCTAT GAGCACAGTG
    TTGTGTGACT CTTCTGGAAA ATTCCGCATG TACAGCAAAG GGGCCTCTGA AATCATCTTG
    CGCAAATGCA CCCGAATCCT GGACCAAGGA GGAGATGTGT GCCCCTTTAA AGCAAAGGAC
    CGGGATGAGA TGGTAAAGAA AGTAATTGAA CCTATGGCAT GCGATGGCTT GCGCACAATC
    TGCCTAGCAT ATCGTGACTT CCCTTCAGAT AGTGAGCCTA ATTGGGATAA TGAGGGCGAC
    ATCCTCTCTG ACCTTATTTG CATTGCTGTA GTAGGAATAG AAGATCCTGT TCGACCAGAG
    GTGCCTGAAG CAATTCAAAA GTGCCAGCGT GCAGGTATCA CAGTGCGCAT GGTTACTGGG
    GACAATATAA ACACAGCTCG AGCAATAGCT ACCAAGTGTG GTATCCTGCA GCCAGGAGAA
    GACTTCTTGT GCTTGGAGGG AAAAGAATTC AACACACTGA TCCGAAATGA AAAGGGAGAG
    GTAGAACAGG ATAAATTGGA CAAGGTTTGG CCGAGACTTC GTGTTTTGGC CCGGTCATCA
    CCTACAGATA AACATACTCT GGTAAAAGGC ATCATTGACA GCACAGTGGC AGAGCGAAGA
    CAGGTGGTGG CTGTGACTGG AGATGGAACA AACGATGGAC CTGCCCTGAA GAAAGCAGAT
    GTGGGATTTG CAATGGGCAT AGCTGGCACA GATGTTGCAA AGGAGGCTTC TGATATTATC
    TTGACAGACG ACAATTTCAC CAGCATTGTA AAGGCTGTAA TGTGGGGCCG CAATGTATAC
    GACAGTATTT CGAAGTTCCT GCAGTTCCAG CTCACCGTAA ATGTTGTGGC AGTAATTGTG
    GCATTTACAG GAGCCTGCAT AACTCAGGAT TCACCACTGA AAGCTGTGCA GATGCTGTGG
    GTCAACCTTA TCATGGATAC ATTTGCATCT TTAGCTTTGG CGACTGAGCC TCCTACTGAT
    TCGCTTCTTC TACGCAAACC ATATGGGCGC AACAAACCCC TGATCTCACG TACAATGATG
    AAAAACATAT TGGGACATGC TGTTTATCAG CTTACCATCA TATTTACATT GCTTTTTGCA
    GGTGAGAAGT TTTTTGACAT TGACAGTGGG AGAAATGTAC CCCTGCATTC TCCACCTTCT
    GAACATTATA CAATTGTCTT CAACACCTTT GTGATGATGC AGCTTTTTAA TGAGATCAAT
    GCCCGCAAGA TCCACGGTGA ACGTAATGTC TTTGAGAACA TCTTCCGGAA CCCCATCTTT
    TGTGCAGTTG TGCTGGGCAC TTTTGGAGCT CAGATAATAA TTGTGGAGTT TGGAGGAAAG
    CCATTCAGTT GCTCTGGCCT TACTTTGTCA CAGTGGTTCT GGTGTATCTT CATTGGGGTT
    GGAGAGCTGT TGTGGGGACA GATGATTTGC ACAATACCCA CCAGCCGACT CAAGTTCCTA
    AAAGAGGCTG GCCATGGGCC ACTGAAGGAG GACATTCAGC AGGAGGAACT TACAGAGGAT
    GTAGATGAGA TTGACCATGC AGAAATGGAG CTTAGGAGAG GCCAGATTTT GTGGTTCAGA
    GGCCTAAACC GAATACAGAC TCAGATTAAA GTTGTGAATG CGTTCCGAAG CTCTCTCTAC
    GAGGGGCTAG AGAAGCCACA GATCCGCAGT GCCATCCATA GCTTCATGAC CCACCCAGAG
    TTCACTCCAC GGGAGGCAGG ACCTGCCCCC CAATTCGAAG ACAGCGACAG CGAGGGTGAG
    GAATCTAAGG GTCTCTCAAA GGCGCATAAA GATCAAACCC ATAATCACAA CAACAATTCT
    TCTGTTGACC CACATGCATT GGATATCACA GTGTCTCTAT CTGATAGCCC ATTGCATAGC
    ATGGAAACCT CCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa03801
    Clone ID Related Accession (Same CDS sequence) XM_004910677.3 , XM_012970635.2 , XM_012970637.2 , XM_012970637.3 , XM_004910677.4 , XM_012970635.3
    Accession Version XM_004910677.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3618bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product plasma membrane calcium-transporting ATPase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004910677.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATA ACTCCACAGA CCATCATTCT GCTAACATTG TAAATGAAGG CAGCTATGAA 
    GGAGATTTTG GCTGCACAAT AATGGACTTA CGGGAGCTTA TGGAATTACG CAGTGCAGAG
    GCAGTCAACC GTATCAGGGA TACTTATGGA GGGGTTCACA ACATCTGCCG CAGGCTAAAG
    ACTTCACCTG TAGAAGGTTT GTCAGGTAAC CCCTCAGACC TCGAGAGGAG GAGACAAATA
    TTTGGTAAAA ACTTCATCCC TCCAAAAAAA GCCAAAACTT TCTTGCAGCT GGTGTGGGAA
    GCACTGCAGG ACGTTACCTT AATTATCCTG GAAATAGCAG CCATAATATC TCTGGGTCTT
    TCTTTCTACC ACCCACCAGG AGGAGATAAT GAATTATGTG GGGAAGCTGC TGCAGGAGTG
    GAGGATGAAG GTGAGGCTCA AGCTGGATGG ATTGAAGGGG CAGCCATCTT ATTCTCGGTC
    ATCATAGTTG TGCTTGTTAC CGCCTTCAAT GACTGGAGCA AAGAGAAGCA ATTTCGAGGC
    CTACAGAGCC GAATTGAGCA GGAGCAGAAA TTCACTGTTA TCCGCAAAGG ACAGGTTATC
    CAAATCCCTG TAGCAGAGCT AGTAGTCGGT GATATTGCAC AAATAAAATA TGGTGACCTC
    CTTCCTGCTG ATGGGATATT GATTCAAGGC AATGACTTAA AAATTGATGA AAGCTCTCTA
    ACTGGGGAGA GCGATCAGGT GAAAAAATCA TTAGAGAAAG ACCCCATGCT TTTGTCAGGA
    ACACACGTAA TGGAAGGGTC TGGACGTATG GTAGTCACAG CAGTAGGTGT CAACTCTCAA
    ACTGGCATAA TATTCACTTT GCTAGGTGCA AATGAGGGAG GTGAAGAGGA AAAAAAATCC
    AAAAAAGGTA AAAAACCAGG ACCCTCAGAA AATCGCAACA AAGCCAAAGC TCAAGATGGT
    GTTGCATTAG AGATCCAACC ACTGAAAAGT GAAGAGGGCG TAGAGTGTGA AGAGAAAGAG
    AAAAAGGTTG TGAAAGTGCC AAAGAAAGAA AAGTCTGTCC TTCAGGGGAA GCTAACTCGG
    CTGGCAGTGC AAATTGGAAA AGCAGGTCTC ATTATGTCAG CCATCACAGT GATCATTTTG
    GTGCTCTATT TTGTCATTTA TACATTTGGA GTGTTAGGTC GCCCATGGTT GGCAGAATGC
    ACTCCCATCT ACATCCAGTA CTTTGTCAAA TTTTTCATTA TTGGTGTCAC TGTGTTGGTG
    GTGGCTGTCC CTGAGGGGCT GCCTCTCGCT GTAACAATAT CTTTGGCCTA CTCTGTAAAG
    AAAATGATGA AAGACAATAA TCTTGTACGT CACTTGGATG CCTGTGAAAC TATGGGCAAT
    GCAACAGCCA TATGCTCTGA TAAAACTGGA ACGCTCACTA TGAACCGAAT GACTGTAGTA
    CAGGCATTTG TTGGTGGTAC TCATTATAGG CAGATTCCTG ATCCGGAGGC ACTGAACACA
    AAAATTCTTG ATCTTATAGT AAATGGGATT TCAGTCAACT CAGCATACAC CTCCAAGATC
    CTGCCCCCTG AAAAGGAGGG AGGTCTCCCC AGGCAGGTGG GTAACAAGAC AGAATGTGCG
    CTACTGGGAT TTGTTTTGGA CCTGAAGCAA GATTATCAGA CAGTGCGAAA CGAAATTCCT
    GAAGAGAACC TCTACAAAGT TTATACGTTC AATTCTGTGC GAAAATCTAT GAGCACAGTG
    TTGTGTGACT CTTCTGGAAA ATTCCGCATG TACAGCAAAG GGGCCTCTGA AATCATCTTG
    CGCAAATGCA CCCGAATCCT GGACCAAGGA GGAGATGTGT GCCCCTTTAA AGCAAAGGAC
    CGGGATGAGA TGGTAAAGAA AGTAATTGAA CCTATGGCAT GCGATGGCTT GCGCACAATC
    TGCCTAGCAT ATCGTGACTT CCCTTCAGAT AGTGAGCCTA ATTGGGATAA TGAGGGCGAC
    ATCCTCTCTG ACCTTATTTG CATTGCTGTA GTAGGAATAG AAGATCCTGT TCGACCAGAG
    GTGCCTGAAG CAATTCAAAA GTGCCAGCGT GCAGGTATCA CAGTGCGCAT GGTTACTGGG
    GACAATATAA ACACAGCTCG AGCAATAGCT ACCAAGTGTG GTATCCTGCA GCCAGGAGAA
    GACTTCTTGT GCTTGGAGGG AAAAGAATTC AACACACTGA TCCGAAATGA AAAGGGAGAG
    GTAGAACAGG ATAAATTGGA CAAGGTTTGG CCGAGACTTC GTGTTTTGGC CCGGTCATCA
    CCTACAGATA AACATACTCT GGTAAAAGGC ATCATTGACA GCACAGTGGC AGAGCGAAGA
    CAGGTGGTGG CTGTGACTGG AGATGGAACA AACGATGGAC CTGCCCTGAA GAAAGCAGAT
    GTGGGATTTG CAATGGGCAT AGCTGGCACA GATGTTGCAA AGGAGGCTTC TGATATTATC
    TTGACAGACG ACAATTTCAC CAGCATTGTA AAGGCTGTAA TGTGGGGCCG CAATGTATAC
    GACAGTATTT CGAAGTTCCT GCAGTTCCAG CTCACCGTAA ATGTTGTGGC AGTAATTGTG
    GCATTTACAG GAGCCTGCAT AACTCAGGAT TCACCACTGA AAGCTGTGCA GATGCTGTGG
    GTCAACCTTA TCATGGATAC ATTTGCATCT TTAGCTTTGG CGACTGAGCC TCCTACTGAT
    TCGCTTCTTC TACGCAAACC ATATGGGCGC AACAAACCCC TGATCTCACG TACAATGATG
    AAAAACATAT TGGGACATGC TGTTTATCAG CTTACCATCA TATTTACATT GCTTTTTGCA
    GGTGAGAAGT TTTTTGACAT TGACAGTGGG AGAAATGTAC CCCTGCATTC TCCACCTTCT
    GAACATTATA CAATTGTCTT CAACACCTTT GTGATGATGC AGCTTTTTAA TGAGATCAAT
    GCCCGCAAGA TCCACGGTGA ACGTAATGTC TTTGAGAACA TCTTCCGGAA CCCCATCTTT
    TGTGCAGTTG TGCTGGGCAC TTTTGGAGCT CAGATAATAA TTGTGGAGTT TGGAGGAAAG
    CCATTCAGTT GCTCTGGCCT TACTTTGTCA CAGTGGTTCT GGTGTATCTT CATTGGGGTT
    GGAGAGCTGT TGTGGGGACA GATGATTTGC ACAATACCCA CCAGCCGACT CAAGTTCCTA
    AAAGAGGCTG GCCATGGGCC ACTGAAGGAG GACATTCAGC AGGAGGAACT TACAGAGGAT
    GTAGATGAGA TTGACCATGC AGAAATGGAG CTTAGGAGAG GCCAGATTTT GTGGTTCAGA
    GGCCTAAACC GAATACAGAC TCAGATTAAA GTTGTGAATG CGTTCCGAAG CTCTCTCTAC
    GAGGGGCTAG AGAAGCCACA GATCCGCAGT GCCATCCATA GCTTCATGAC CCACCCAGAG
    TTCACTCCAC GGGAGGCAGG ACCTGCCCCC CAATTCGAAG ACAGCGACAG CGAGGGTGAG
    GAATCTAAGG GTCTCTCAAA GGCGCATAAA GATCAAACCC ATAATCACAA CAACAATTCT
    TCTGTTGACC CACATGCATT GGATATCACA GTGTCTCTAT CTGATAGCCC ATTGCATAGC
    ATGGAAACCT CCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004910734.1
    CDS664..4281
    Translation

    Target ORF information:

    RefSeq Version XM_004910677.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis plasma membrane calcium-transporting ATPase 4 (LOC100488346), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004910677.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATA ACTCCACAGA CCATCATTCT GCTAACATTG TAAATGAAGG CAGCTATGAA 
    GGAGATTTTG GCTGCACAAT AATGGACTTA CGGGAGCTTA TGGAATTACG CAGTGCAGAG
    GCAGTCAACC GTATCAGGGA TACTTATGGA GGGGTTCACA ACATCTGCCG CAGGCTAAAG
    ACTTCACCTG TAGAAGGTTT GTCAGGTAAC CCCTCAGACC TCGAGAGGAG GAGACAAATA
    TTTGGTAAAA ACTTCATCCC TCCAAAAAAA GCCAAAACTT TCTTGCAGCT GGTGTGGGAA
    GCACTGCAGG ACGTTACCTT AATTATCCTG GAAATAGCAG CCATAATATC TCTGGGTCTT
    TCTTTCTACC ACCCACCAGG AGGAGATAAT GAATTATGTG GGGAAGCTGC TGCAGGAGTG
    GAGGATGAAG GTGAGGCTCA AGCTGGATGG ATTGAAGGGG CAGCCATCTT ATTCTCGGTC
    ATCATAGTTG TGCTTGTTAC CGCCTTCAAT GACTGGAGCA AAGAGAAGCA ATTTCGAGGC
    CTACAGAGCC GAATTGAGCA GGAGCAGAAA TTCACTGTTA TCCGCAAAGG ACAGGTTATC
    CAAATCCCTG TAGCAGAGCT AGTAGTCGGT GATATTGCAC AAATAAAATA TGGTGACCTC
    CTTCCTGCTG ATGGGATATT GATTCAAGGC AATGACTTAA AAATTGATGA AAGCTCTCTA
    ACTGGGGAGA GCGATCAGGT GAAAAAATCA TTAGAGAAAG ACCCCATGCT TTTGTCAGGA
    ACACACGTAA TGGAAGGGTC TGGACGTATG GTAGTCACAG CAGTAGGTGT CAACTCTCAA
    ACTGGCATAA TATTCACTTT GCTAGGTGCA AATGAGGGAG GTGAAGAGGA AAAAAAATCC
    AAAAAAGGTA AAAAACCAGG ACCCTCAGAA AATCGCAACA AAGCCAAAGC TCAAGATGGT
    GTTGCATTAG AGATCCAACC ACTGAAAAGT GAAGAGGGCG TAGAGTGTGA AGAGAAAGAG
    AAAAAGGTTG TGAAAGTGCC AAAGAAAGAA AAGTCTGTCC TTCAGGGGAA GCTAACTCGG
    CTGGCAGTGC AAATTGGAAA AGCAGGTCTC ATTATGTCAG CCATCACAGT GATCATTTTG
    GTGCTCTATT TTGTCATTTA TACATTTGGA GTGTTAGGTC GCCCATGGTT GGCAGAATGC
    ACTCCCATCT ACATCCAGTA CTTTGTCAAA TTTTTCATTA TTGGTGTCAC TGTGTTGGTG
    GTGGCTGTCC CTGAGGGGCT GCCTCTCGCT GTAACAATAT CTTTGGCCTA CTCTGTAAAG
    AAAATGATGA AAGACAATAA TCTTGTACGT CACTTGGATG CCTGTGAAAC TATGGGCAAT
    GCAACAGCCA TATGCTCTGA TAAAACTGGA ACGCTCACTA TGAACCGAAT GACTGTAGTA
    CAGGCATTTG TTGGTGGTAC TCATTATAGG CAGATTCCTG ATCCGGAGGC ACTGAACACA
    AAAATTCTTG ATCTTATAGT AAATGGGATT TCAGTCAACT CAGCATACAC CTCCAAGATC
    CTGCCCCCTG AAAAGGAGGG AGGTCTCCCC AGGCAGGTGG GTAACAAGAC AGAATGTGCG
    CTACTGGGAT TTGTTTTGGA CCTGAAGCAA GATTATCAGA CAGTGCGAAA CGAAATTCCT
    GAAGAGAACC TCTACAAAGT TTATACGTTC AATTCTGTGC GAAAATCTAT GAGCACAGTG
    TTGTGTGACT CTTCTGGAAA ATTCCGCATG TACAGCAAAG GGGCCTCTGA AATCATCTTG
    CGCAAATGCA CCCGAATCCT GGACCAAGGA GGAGATGTGT GCCCCTTTAA AGCAAAGGAC
    CGGGATGAGA TGGTAAAGAA AGTAATTGAA CCTATGGCAT GCGATGGCTT GCGCACAATC
    TGCCTAGCAT ATCGTGACTT CCCTTCAGAT AGTGAGCCTA ATTGGGATAA TGAGGGCGAC
    ATCCTCTCTG ACCTTATTTG CATTGCTGTA GTAGGAATAG AAGATCCTGT TCGACCAGAG
    GTGCCTGAAG CAATTCAAAA GTGCCAGCGT GCAGGTATCA CAGTGCGCAT GGTTACTGGG
    GACAATATAA ACACAGCTCG AGCAATAGCT ACCAAGTGTG GTATCCTGCA GCCAGGAGAA
    GACTTCTTGT GCTTGGAGGG AAAAGAATTC AACACACTGA TCCGAAATGA AAAGGGAGAG
    GTAGAACAGG ATAAATTGGA CAAGGTTTGG CCGAGACTTC GTGTTTTGGC CCGGTCATCA
    CCTACAGATA AACATACTCT GGTAAAAGGC ATCATTGACA GCACAGTGGC AGAGCGAAGA
    CAGGTGGTGG CTGTGACTGG AGATGGAACA AACGATGGAC CTGCCCTGAA GAAAGCAGAT
    GTGGGATTTG CAATGGGCAT AGCTGGCACA GATGTTGCAA AGGAGGCTTC TGATATTATC
    TTGACAGACG ACAATTTCAC CAGCATTGTA AAGGCTGTAA TGTGGGGCCG CAATGTATAC
    GACAGTATTT CGAAGTTCCT GCAGTTCCAG CTCACCGTAA ATGTTGTGGC AGTAATTGTG
    GCATTTACAG GAGCCTGCAT AACTCAGGAT TCACCACTGA AAGCTGTGCA GATGCTGTGG
    GTCAACCTTA TCATGGATAC ATTTGCATCT TTAGCTTTGG CGACTGAGCC TCCTACTGAT
    TCGCTTCTTC TACGCAAACC ATATGGGCGC AACAAACCCC TGATCTCACG TACAATGATG
    AAAAACATAT TGGGACATGC TGTTTATCAG CTTACCATCA TATTTACATT GCTTTTTGCA
    GGTGAGAAGT TTTTTGACAT TGACAGTGGG AGAAATGTAC CCCTGCATTC TCCACCTTCT
    GAACATTATA CAATTGTCTT CAACACCTTT GTGATGATGC AGCTTTTTAA TGAGATCAAT
    GCCCGCAAGA TCCACGGTGA ACGTAATGTC TTTGAGAACA TCTTCCGGAA CCCCATCTTT
    TGTGCAGTTG TGCTGGGCAC TTTTGGAGCT CAGATAATAA TTGTGGAGTT TGGAGGAAAG
    CCATTCAGTT GCTCTGGCCT TACTTTGTCA CAGTGGTTCT GGTGTATCTT CATTGGGGTT
    GGAGAGCTGT TGTGGGGACA GATGATTTGC ACAATACCCA CCAGCCGACT CAAGTTCCTA
    AAAGAGGCTG GCCATGGGCC ACTGAAGGAG GACATTCAGC AGGAGGAACT TACAGAGGAT
    GTAGATGAGA TTGACCATGC AGAAATGGAG CTTAGGAGAG GCCAGATTTT GTGGTTCAGA
    GGCCTAAACC GAATACAGAC TCAGATTAAA GTTGTGAATG CGTTCCGAAG CTCTCTCTAC
    GAGGGGCTAG AGAAGCCACA GATCCGCAGT GCCATCCATA GCTTCATGAC CCACCCAGAG
    TTCACTCCAC GGGAGGCAGG ACCTGCCCCC CAATTCGAAG ACAGCGACAG CGAGGGTGAG
    GAATCTAAGG GTCTCTCAAA GGCGCATAAA GATCAAACCC ATAATCACAA CAACAATTCT
    TCTGTTGACC CACATGCATT GGATATCACA GTGTCTCTAT CTGATAGCCC ATTGCATAGC
    ATGGAAACCT CCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa03801
    Clone ID Related Accession (Same CDS sequence) XM_004910677.3 , XM_012970635.2 , XM_012970637.2 , XM_012970637.3 , XM_004910677.4 , XM_012970635.3
    Accession Version XM_012970635.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3618bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product plasma membrane calcium-transporting ATPase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970635.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATA ACTCCACAGA CCATCATTCT GCTAACATTG TAAATGAAGG CAGCTATGAA 
    GGAGATTTTG GCTGCACAAT AATGGACTTA CGGGAGCTTA TGGAATTACG CAGTGCAGAG
    GCAGTCAACC GTATCAGGGA TACTTATGGA GGGGTTCACA ACATCTGCCG CAGGCTAAAG
    ACTTCACCTG TAGAAGGTTT GTCAGGTAAC CCCTCAGACC TCGAGAGGAG GAGACAAATA
    TTTGGTAAAA ACTTCATCCC TCCAAAAAAA GCCAAAACTT TCTTGCAGCT GGTGTGGGAA
    GCACTGCAGG ACGTTACCTT AATTATCCTG GAAATAGCAG CCATAATATC TCTGGGTCTT
    TCTTTCTACC ACCCACCAGG AGGAGATAAT GAATTATGTG GGGAAGCTGC TGCAGGAGTG
    GAGGATGAAG GTGAGGCTCA AGCTGGATGG ATTGAAGGGG CAGCCATCTT ATTCTCGGTC
    ATCATAGTTG TGCTTGTTAC CGCCTTCAAT GACTGGAGCA AAGAGAAGCA ATTTCGAGGC
    CTACAGAGCC GAATTGAGCA GGAGCAGAAA TTCACTGTTA TCCGCAAAGG ACAGGTTATC
    CAAATCCCTG TAGCAGAGCT AGTAGTCGGT GATATTGCAC AAATAAAATA TGGTGACCTC
    CTTCCTGCTG ATGGGATATT GATTCAAGGC AATGACTTAA AAATTGATGA AAGCTCTCTA
    ACTGGGGAGA GCGATCAGGT GAAAAAATCA TTAGAGAAAG ACCCCATGCT TTTGTCAGGA
    ACACACGTAA TGGAAGGGTC TGGACGTATG GTAGTCACAG CAGTAGGTGT CAACTCTCAA
    ACTGGCATAA TATTCACTTT GCTAGGTGCA AATGAGGGAG GTGAAGAGGA AAAAAAATCC
    AAAAAAGGTA AAAAACCAGG ACCCTCAGAA AATCGCAACA AAGCCAAAGC TCAAGATGGT
    GTTGCATTAG AGATCCAACC ACTGAAAAGT GAAGAGGGCG TAGAGTGTGA AGAGAAAGAG
    AAAAAGGTTG TGAAAGTGCC AAAGAAAGAA AAGTCTGTCC TTCAGGGGAA GCTAACTCGG
    CTGGCAGTGC AAATTGGAAA AGCAGGTCTC ATTATGTCAG CCATCACAGT GATCATTTTG
    GTGCTCTATT TTGTCATTTA TACATTTGGA GTGTTAGGTC GCCCATGGTT GGCAGAATGC
    ACTCCCATCT ACATCCAGTA CTTTGTCAAA TTTTTCATTA TTGGTGTCAC TGTGTTGGTG
    GTGGCTGTCC CTGAGGGGCT GCCTCTCGCT GTAACAATAT CTTTGGCCTA CTCTGTAAAG
    AAAATGATGA AAGACAATAA TCTTGTACGT CACTTGGATG CCTGTGAAAC TATGGGCAAT
    GCAACAGCCA TATGCTCTGA TAAAACTGGA ACGCTCACTA TGAACCGAAT GACTGTAGTA
    CAGGCATTTG TTGGTGGTAC TCATTATAGG CAGATTCCTG ATCCGGAGGC ACTGAACACA
    AAAATTCTTG ATCTTATAGT AAATGGGATT TCAGTCAACT CAGCATACAC CTCCAAGATC
    CTGCCCCCTG AAAAGGAGGG AGGTCTCCCC AGGCAGGTGG GTAACAAGAC AGAATGTGCG
    CTACTGGGAT TTGTTTTGGA CCTGAAGCAA GATTATCAGA CAGTGCGAAA CGAAATTCCT
    GAAGAGAACC TCTACAAAGT TTATACGTTC AATTCTGTGC GAAAATCTAT GAGCACAGTG
    TTGTGTGACT CTTCTGGAAA ATTCCGCATG TACAGCAAAG GGGCCTCTGA AATCATCTTG
    CGCAAATGCA CCCGAATCCT GGACCAAGGA GGAGATGTGT GCCCCTTTAA AGCAAAGGAC
    CGGGATGAGA TGGTAAAGAA AGTAATTGAA CCTATGGCAT GCGATGGCTT GCGCACAATC
    TGCCTAGCAT ATCGTGACTT CCCTTCAGAT AGTGAGCCTA ATTGGGATAA TGAGGGCGAC
    ATCCTCTCTG ACCTTATTTG CATTGCTGTA GTAGGAATAG AAGATCCTGT TCGACCAGAG
    GTGCCTGAAG CAATTCAAAA GTGCCAGCGT GCAGGTATCA CAGTGCGCAT GGTTACTGGG
    GACAATATAA ACACAGCTCG AGCAATAGCT ACCAAGTGTG GTATCCTGCA GCCAGGAGAA
    GACTTCTTGT GCTTGGAGGG AAAAGAATTC AACACACTGA TCCGAAATGA AAAGGGAGAG
    GTAGAACAGG ATAAATTGGA CAAGGTTTGG CCGAGACTTC GTGTTTTGGC CCGGTCATCA
    CCTACAGATA AACATACTCT GGTAAAAGGC ATCATTGACA GCACAGTGGC AGAGCGAAGA
    CAGGTGGTGG CTGTGACTGG AGATGGAACA AACGATGGAC CTGCCCTGAA GAAAGCAGAT
    GTGGGATTTG CAATGGGCAT AGCTGGCACA GATGTTGCAA AGGAGGCTTC TGATATTATC
    TTGACAGACG ACAATTTCAC CAGCATTGTA AAGGCTGTAA TGTGGGGCCG CAATGTATAC
    GACAGTATTT CGAAGTTCCT GCAGTTCCAG CTCACCGTAA ATGTTGTGGC AGTAATTGTG
    GCATTTACAG GAGCCTGCAT AACTCAGGAT TCACCACTGA AAGCTGTGCA GATGCTGTGG
    GTCAACCTTA TCATGGATAC ATTTGCATCT TTAGCTTTGG CGACTGAGCC TCCTACTGAT
    TCGCTTCTTC TACGCAAACC ATATGGGCGC AACAAACCCC TGATCTCACG TACAATGATG
    AAAAACATAT TGGGACATGC TGTTTATCAG CTTACCATCA TATTTACATT GCTTTTTGCA
    GGTGAGAAGT TTTTTGACAT TGACAGTGGG AGAAATGTAC CCCTGCATTC TCCACCTTCT
    GAACATTATA CAATTGTCTT CAACACCTTT GTGATGATGC AGCTTTTTAA TGAGATCAAT
    GCCCGCAAGA TCCACGGTGA ACGTAATGTC TTTGAGAACA TCTTCCGGAA CCCCATCTTT
    TGTGCAGTTG TGCTGGGCAC TTTTGGAGCT CAGATAATAA TTGTGGAGTT TGGAGGAAAG
    CCATTCAGTT GCTCTGGCCT TACTTTGTCA CAGTGGTTCT GGTGTATCTT CATTGGGGTT
    GGAGAGCTGT TGTGGGGACA GATGATTTGC ACAATACCCA CCAGCCGACT CAAGTTCCTA
    AAAGAGGCTG GCCATGGGCC ACTGAAGGAG GACATTCAGC AGGAGGAACT TACAGAGGAT
    GTAGATGAGA TTGACCATGC AGAAATGGAG CTTAGGAGAG GCCAGATTTT GTGGTTCAGA
    GGCCTAAACC GAATACAGAC TCAGATTAAA GTTGTGAATG CGTTCCGAAG CTCTCTCTAC
    GAGGGGCTAG AGAAGCCACA GATCCGCAGT GCCATCCATA GCTTCATGAC CCACCCAGAG
    TTCACTCCAC GGGAGGCAGG ACCTGCCCCC CAATTCGAAG ACAGCGACAG CGAGGGTGAG
    GAATCTAAGG GTCTCTCAAA GGCGCATAAA GATCAAACCC ATAATCACAA CAACAATTCT
    TCTGTTGACC CACATGCATT GGATATCACA GTGTCTCTAT CTGATAGCCC ATTGCATAGC
    ATGGAAACCT CCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012826089.1
    CDS744..4361
    Translation

    Target ORF information:

    RefSeq Version XM_012970635.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis plasma membrane calcium-transporting ATPase 4 (LOC100488346), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970635.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATA ACTCCACAGA CCATCATTCT GCTAACATTG TAAATGAAGG CAGCTATGAA 
    GGAGATTTTG GCTGCACAAT AATGGACTTA CGGGAGCTTA TGGAATTACG CAGTGCAGAG
    GCAGTCAACC GTATCAGGGA TACTTATGGA GGGGTTCACA ACATCTGCCG CAGGCTAAAG
    ACTTCACCTG TAGAAGGTTT GTCAGGTAAC CCCTCAGACC TCGAGAGGAG GAGACAAATA
    TTTGGTAAAA ACTTCATCCC TCCAAAAAAA GCCAAAACTT TCTTGCAGCT GGTGTGGGAA
    GCACTGCAGG ACGTTACCTT AATTATCCTG GAAATAGCAG CCATAATATC TCTGGGTCTT
    TCTTTCTACC ACCCACCAGG AGGAGATAAT GAATTATGTG GGGAAGCTGC TGCAGGAGTG
    GAGGATGAAG GTGAGGCTCA AGCTGGATGG ATTGAAGGGG CAGCCATCTT ATTCTCGGTC
    ATCATAGTTG TGCTTGTTAC CGCCTTCAAT GACTGGAGCA AAGAGAAGCA ATTTCGAGGC
    CTACAGAGCC GAATTGAGCA GGAGCAGAAA TTCACTGTTA TCCGCAAAGG ACAGGTTATC
    CAAATCCCTG TAGCAGAGCT AGTAGTCGGT GATATTGCAC AAATAAAATA TGGTGACCTC
    CTTCCTGCTG ATGGGATATT GATTCAAGGC AATGACTTAA AAATTGATGA AAGCTCTCTA
    ACTGGGGAGA GCGATCAGGT GAAAAAATCA TTAGAGAAAG ACCCCATGCT TTTGTCAGGA
    ACACACGTAA TGGAAGGGTC TGGACGTATG GTAGTCACAG CAGTAGGTGT CAACTCTCAA
    ACTGGCATAA TATTCACTTT GCTAGGTGCA AATGAGGGAG GTGAAGAGGA AAAAAAATCC
    AAAAAAGGTA AAAAACCAGG ACCCTCAGAA AATCGCAACA AAGCCAAAGC TCAAGATGGT
    GTTGCATTAG AGATCCAACC ACTGAAAAGT GAAGAGGGCG TAGAGTGTGA AGAGAAAGAG
    AAAAAGGTTG TGAAAGTGCC AAAGAAAGAA AAGTCTGTCC TTCAGGGGAA GCTAACTCGG
    CTGGCAGTGC AAATTGGAAA AGCAGGTCTC ATTATGTCAG CCATCACAGT GATCATTTTG
    GTGCTCTATT TTGTCATTTA TACATTTGGA GTGTTAGGTC GCCCATGGTT GGCAGAATGC
    ACTCCCATCT ACATCCAGTA CTTTGTCAAA TTTTTCATTA TTGGTGTCAC TGTGTTGGTG
    GTGGCTGTCC CTGAGGGGCT GCCTCTCGCT GTAACAATAT CTTTGGCCTA CTCTGTAAAG
    AAAATGATGA AAGACAATAA TCTTGTACGT CACTTGGATG CCTGTGAAAC TATGGGCAAT
    GCAACAGCCA TATGCTCTGA TAAAACTGGA ACGCTCACTA TGAACCGAAT GACTGTAGTA
    CAGGCATTTG TTGGTGGTAC TCATTATAGG CAGATTCCTG ATCCGGAGGC ACTGAACACA
    AAAATTCTTG ATCTTATAGT AAATGGGATT TCAGTCAACT CAGCATACAC CTCCAAGATC
    CTGCCCCCTG AAAAGGAGGG AGGTCTCCCC AGGCAGGTGG GTAACAAGAC AGAATGTGCG
    CTACTGGGAT TTGTTTTGGA CCTGAAGCAA GATTATCAGA CAGTGCGAAA CGAAATTCCT
    GAAGAGAACC TCTACAAAGT TTATACGTTC AATTCTGTGC GAAAATCTAT GAGCACAGTG
    TTGTGTGACT CTTCTGGAAA ATTCCGCATG TACAGCAAAG GGGCCTCTGA AATCATCTTG
    CGCAAATGCA CCCGAATCCT GGACCAAGGA GGAGATGTGT GCCCCTTTAA AGCAAAGGAC
    CGGGATGAGA TGGTAAAGAA AGTAATTGAA CCTATGGCAT GCGATGGCTT GCGCACAATC
    TGCCTAGCAT ATCGTGACTT CCCTTCAGAT AGTGAGCCTA ATTGGGATAA TGAGGGCGAC
    ATCCTCTCTG ACCTTATTTG CATTGCTGTA GTAGGAATAG AAGATCCTGT TCGACCAGAG
    GTGCCTGAAG CAATTCAAAA GTGCCAGCGT GCAGGTATCA CAGTGCGCAT GGTTACTGGG
    GACAATATAA ACACAGCTCG AGCAATAGCT ACCAAGTGTG GTATCCTGCA GCCAGGAGAA
    GACTTCTTGT GCTTGGAGGG AAAAGAATTC AACACACTGA TCCGAAATGA AAAGGGAGAG
    GTAGAACAGG ATAAATTGGA CAAGGTTTGG CCGAGACTTC GTGTTTTGGC CCGGTCATCA
    CCTACAGATA AACATACTCT GGTAAAAGGC ATCATTGACA GCACAGTGGC AGAGCGAAGA
    CAGGTGGTGG CTGTGACTGG AGATGGAACA AACGATGGAC CTGCCCTGAA GAAAGCAGAT
    GTGGGATTTG CAATGGGCAT AGCTGGCACA GATGTTGCAA AGGAGGCTTC TGATATTATC
    TTGACAGACG ACAATTTCAC CAGCATTGTA AAGGCTGTAA TGTGGGGCCG CAATGTATAC
    GACAGTATTT CGAAGTTCCT GCAGTTCCAG CTCACCGTAA ATGTTGTGGC AGTAATTGTG
    GCATTTACAG GAGCCTGCAT AACTCAGGAT TCACCACTGA AAGCTGTGCA GATGCTGTGG
    GTCAACCTTA TCATGGATAC ATTTGCATCT TTAGCTTTGG CGACTGAGCC TCCTACTGAT
    TCGCTTCTTC TACGCAAACC ATATGGGCGC AACAAACCCC TGATCTCACG TACAATGATG
    AAAAACATAT TGGGACATGC TGTTTATCAG CTTACCATCA TATTTACATT GCTTTTTGCA
    GGTGAGAAGT TTTTTGACAT TGACAGTGGG AGAAATGTAC CCCTGCATTC TCCACCTTCT
    GAACATTATA CAATTGTCTT CAACACCTTT GTGATGATGC AGCTTTTTAA TGAGATCAAT
    GCCCGCAAGA TCCACGGTGA ACGTAATGTC TTTGAGAACA TCTTCCGGAA CCCCATCTTT
    TGTGCAGTTG TGCTGGGCAC TTTTGGAGCT CAGATAATAA TTGTGGAGTT TGGAGGAAAG
    CCATTCAGTT GCTCTGGCCT TACTTTGTCA CAGTGGTTCT GGTGTATCTT CATTGGGGTT
    GGAGAGCTGT TGTGGGGACA GATGATTTGC ACAATACCCA CCAGCCGACT CAAGTTCCTA
    AAAGAGGCTG GCCATGGGCC ACTGAAGGAG GACATTCAGC AGGAGGAACT TACAGAGGAT
    GTAGATGAGA TTGACCATGC AGAAATGGAG CTTAGGAGAG GCCAGATTTT GTGGTTCAGA
    GGCCTAAACC GAATACAGAC TCAGATTAAA GTTGTGAATG CGTTCCGAAG CTCTCTCTAC
    GAGGGGCTAG AGAAGCCACA GATCCGCAGT GCCATCCATA GCTTCATGAC CCACCCAGAG
    TTCACTCCAC GGGAGGCAGG ACCTGCCCCC CAATTCGAAG ACAGCGACAG CGAGGGTGAG
    GAATCTAAGG GTCTCTCAAA GGCGCATAAA GATCAAACCC ATAATCACAA CAACAATTCT
    TCTGTTGACC CACATGCATT GGATATCACA GTGTCTCTAT CTGATAGCCC ATTGCATAGC
    ATGGAAACCT CCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa03805
    Clone ID Related Accession (Same CDS sequence) XM_002932973.4 , XM_002932973.5
    Accession Version XM_002932973.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3507bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product plasma membrane calcium-transporting ATPase 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002932973.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCAAATA ACTCCACAGA CCATCATTCT GCTAACATTG TAAATGAAGG CAGCTATGAA 
    GGAGATTTTG GCTGCACAAT AATGGACTTA CGGGAGCTTA TGGAATTACG CAGTGCAGAG
    GCAGTCAACC GTATCAGGGA TACTTATGGA GGGGTTCACA ACATCTGCCG CAGGCTAAAG
    ACTTCACCTG TAGAAGGTTT GTCAGGTAAC CCCTCAGACC TCGAGAGGAG GAGACAAATA
    TTTGGTAAAA ACTTCATCCC TCCAAAAAAA GCCAAAACTT TCTTGCAGCT GGTGTGGGAA
    GCACTGCAGG ACGTTACCTT AATTATCCTG GAAATAGCAG CCATAATATC TCTGGGTCTT
    TCTTTCTACC ACCCACCAGG AGGAGATAAT GAATTATGTG GGGAAGCTGC TGCAGGAGTG
    GAGGATGAAG GTGAGGCTCA AGCTGGATGG ATTGAAGGGG CAGCCATCTT ATTCTCGGTC
    ATCATAGTTG TGCTTGTTAC CGCCTTCAAT GACTGGAGCA AAGAGAAGCA ATTTCGAGGC
    CTACAGAGCC GAATTGAGCA GGAGCAGAAA TTCACTGTTA TCCGCAAAGG ACAGGTTATC
    CAAATCCCTG TAGCAGAGCT AGTAGTCGGT GATATTGCAC AAATAAAATA TGGTGACCTC
    CTTCCTGCTG ATGGGATATT GATTCAAGGC AATGACTTAA AAATTGATGA AAGCTCTCTA
    ACTGGGGAGA GCGATCAGGT GAAAAAATCA TTAGAGAAAG ACCCCATGCT TTTGTCAGGA
    ACACACGTAA TGGAAGGGTC TGGACGTATG GTAGTCACAG CAGTAGGTGT CAACTCTCAA
    ACTGGCATAA TATTCACTTT GCTAGGTGCA AATGAGGGAG GTGAAGAGGA AAAAAAATCC
    AAAAAAGGTA AAAAACCAGG ACCCTCAGAA AATCGCAACA AAGCCAAAGC TCAAGATGGT
    GTTGCATTAG AGATCCAACC ACTGAAAAGT GAAGAGGGCG TAGAGTGTGA AGAGAAAGAG
    AAAAAGGTTG TGAAAGTGCC AAAGAAAGAA AAGTCTGTCC TTCAGGGGAA GCTAACTCGG
    CTGGCAGTGC AAATTGGAAA AGCAGGTCTC ATTATGTCAG CCATCACAGT GATCATTTTG
    GTGCTCTATT TTGTCATTTA TACATTTGGA GTGTTAGGTC GCCCATGGTT GGCAGAATGC
    ACTCCCATCT ACATCCAGTA CTTTGTCAAA TTTTTCATTA TTGGTGTCAC TGTGTTGGTG
    GTGGCTGTCC CTGAGGGGCT GCCTCTCGCT GTAACAATAT CTTTGGCCTA CTCTGTAAAG
    AAAATGATGA AAGACAATAA TCTTGTACGT CACTTGGATG CCTGTGAAAC TATGGGCAAT
    GCAACAGCCA TATGCTCTGA TAAAACTGGA ACGCTCACTA TGAACCGAAT GACTGTAGTA
    CAGGCATTTG TTGGTGGTAC TCATTATAGG CAGATTCCTG ATCCGGAGGC ACTGAACACA
    AAAATTCTTG ATCTTATAGT AAATGGGATT TCAGTCAACT CAGCATACAC CTCCAAGATC
    CTGCCCCCTG AAAAGGAGGG AGGTCTCCCC AGGCAGGTGG GTAACAAGAC AGAATGTGCG
    CTACTGGGAT TTGTTTTGGA CCTGAAGCAA GATTATCAGA CAGTGCGAAA CGAAATTCCT
    GAAGAGAACC TCTACAAAGT TTATACGTTC AATTCTGTGC GAAAATCTAT GAGCACAGTG
    TTGTGTGACT CTTCTGGAAA ATTCCGCATG TACAGCAAAG GGGCCTCTGA AATCATCTTG
    CGCAAATGCA CCCGAATCCT GGACCAAGGA GGAGATGTGT GCCCCTTTAA AGCAAAGGAC
    CGGGATGAGA TGGTAAAGAA AGTAATTGAA CCTATGGCAT GCGATGGCTT GCGCACAATC
    TGCCTAGCAT ATCGTGACTT CCCTTCAGAT AGTGAGCCTA ATTGGGATAA TGAGGGCGAC
    ATCCTCTCTG ACCTTATTTG CATTGCTGTA GTAGGAATAG AAGATCCTGT TCGACCAGAG
    GTGCCTGAAG CAATTCAAAA GTGCCAGCGT GCAGGTATCA CAGTGCGCAT GGTTACTGGG
    GACAATATAA ACACAGCTCG AGCAATAGCT ACCAAGTGTG GTATCCTGCA GCCAGGAGAA
    GACTTCTTGT GCTTGGAGGG AAAAGAATTC AACACACTGA TCCGAAATGA AAAGGGAGAG
    GTAGAACAGG ATAAATTGGA CAAGGTTTGG CCGAGACTTC GTGTTTTGGC CCGGTCATCA
    CCTACAGATA AACATACTCT GGTAAAAGGC ATCATTGACA GCACAGTGGC AGAGCGAAGA
    CAGGTGGTGG CTGTGACTGG AGATGGAACA AACGATGGAC CTGCCCTGAA GAAAGCAGAT
    GTGGGATTTG CAATGGGCAT AGCTGGCACA GATGTTGCAA AGGAGGCTTC TGATATTATC
    TTGACAGACG ACAATTTCAC CAGCATTGTA AAGGCTGTAA TGTGGGGCCG CAATGTATAC
    GACAGTATTT CGAAGTTCCT GCAGTTCCAG CTCACCGTAA ATGTTGTGGC AGTAATTGTG
    GCATTTACAG GAGCCTGCAT AACTCAGGAT TCACCACTGA AAGCTGTGCA GATGCTGTGG
    GTCAACCTTA TCATGGATAC ATTTGCATCT TTAGCTTTGG CGACTGAGCC TCCTACTGAT
    TCGCTTCTTC TACGCAAACC ATATGGGCGC AACAAACCCC TGATCTCACG TACAATGATG
    AAAAACATAT TGGGACATGC TGTTTATCAG CTTACCATCA TATTTACATT GCTTTTTGCA
    GGTGAGAAGT TTTTTGACAT TGACAGTGGG AGAAATGTAC CCCTGCATTC TCCACCTTCT
    GAACATTATA CAATTGTCTT CAACACCTTT GTGATGATGC AGCTTTTTAA TGAGATCAAT
    GCCCGCAAGA TCCACGGTGA ACGTAATGTC TTTGAGAACA TCTTCCGGAA CCCCATCTTT
    TGTGCAGTTG TGCTGGGCAC TTTTGGAGCT CAGATAATAA TTGTGGAGTT TGGAGGAAAG
    CCATTCAGTT GCTCTGGCCT TACTTTGTCA CAGTGGTTCT GGTGTATCTT CATTGGGGTT
    GGAGAGCTGT TGTGGGGACA GATGATTTGC ACAATACCCA CCAGCCGACT CAAGTTCCTA
    AAAGAGGCTG GCCATGGGCC ACTGAAGGAG GACATTCAGC AGGAGGAACT TACAGAGGAT
    GTAGATGAGA TTGACCATGC AGAAATGGAG CTTAGGAGAG GCCAGATTTT GTGGTTCAGA
    GGCCTAAACC GAATACAGAC TCAGATGGAC GTAGTTTATA CATTCCAGAC GGGAGGTACC
    TCTTTACAAG GGGCTCTGAG GCGCCAGCCT TCCACCATTA GCCAGCACCT TGATGCTAAA
    CATGCCTCTA GCCCAACACA CGTAGCTCTG TCCTCTACTA CCTCTCCCCC AGCCACTTCT
    GCTGCTGCTG CTTCTGTGGG CAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002933019.1
    CDS666..4172
    Translation

    Target ORF information:

    RefSeq Version XM_002932973.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis plasma membrane calcium-transporting ATPase 4 (LOC100488346), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002932973.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCAAATA ACTCCACAGA CCATCATTCT GCTAACATTG TAAATGAAGG CAGCTATGAA 
    GGAGATTTTG GCTGCACAAT AATGGACTTA CGGGAGCTTA TGGAATTACG CAGTGCAGAG
    GCAGTCAACC GTATCAGGGA TACTTATGGA GGGGTTCACA ACATCTGCCG CAGGCTAAAG
    ACTTCACCTG TAGAAGGTTT GTCAGGTAAC CCCTCAGACC TCGAGAGGAG GAGACAAATA
    TTTGGTAAAA ACTTCATCCC TCCAAAAAAA GCCAAAACTT TCTTGCAGCT GGTGTGGGAA
    GCACTGCAGG ACGTTACCTT AATTATCCTG GAAATAGCAG CCATAATATC TCTGGGTCTT
    TCTTTCTACC ACCCACCAGG AGGAGATAAT GAATTATGTG GGGAAGCTGC TGCAGGAGTG
    GAGGATGAAG GTGAGGCTCA AGCTGGATGG ATTGAAGGGG CAGCCATCTT ATTCTCGGTC
    ATCATAGTTG TGCTTGTTAC CGCCTTCAAT GACTGGAGCA AAGAGAAGCA ATTTCGAGGC
    CTACAGAGCC GAATTGAGCA GGAGCAGAAA TTCACTGTTA TCCGCAAAGG ACAGGTTATC
    CAAATCCCTG TAGCAGAGCT AGTAGTCGGT GATATTGCAC AAATAAAATA TGGTGACCTC
    CTTCCTGCTG ATGGGATATT GATTCAAGGC AATGACTTAA AAATTGATGA AAGCTCTCTA
    ACTGGGGAGA GCGATCAGGT GAAAAAATCA TTAGAGAAAG ACCCCATGCT TTTGTCAGGA
    ACACACGTAA TGGAAGGGTC TGGACGTATG GTAGTCACAG CAGTAGGTGT CAACTCTCAA
    ACTGGCATAA TATTCACTTT GCTAGGTGCA AATGAGGGAG GTGAAGAGGA AAAAAAATCC
    AAAAAAGGTA AAAAACCAGG ACCCTCAGAA AATCGCAACA AAGCCAAAGC TCAAGATGGT
    GTTGCATTAG AGATCCAACC ACTGAAAAGT GAAGAGGGCG TAGAGTGTGA AGAGAAAGAG
    AAAAAGGTTG TGAAAGTGCC AAAGAAAGAA AAGTCTGTCC TTCAGGGGAA GCTAACTCGG
    CTGGCAGTGC AAATTGGAAA AGCAGGTCTC ATTATGTCAG CCATCACAGT GATCATTTTG
    GTGCTCTATT TTGTCATTTA TACATTTGGA GTGTTAGGTC GCCCATGGTT GGCAGAATGC
    ACTCCCATCT ACATCCAGTA CTTTGTCAAA TTTTTCATTA TTGGTGTCAC TGTGTTGGTG
    GTGGCTGTCC CTGAGGGGCT GCCTCTCGCT GTAACAATAT CTTTGGCCTA CTCTGTAAAG
    AAAATGATGA AAGACAATAA TCTTGTACGT CACTTGGATG CCTGTGAAAC TATGGGCAAT
    GCAACAGCCA TATGCTCTGA TAAAACTGGA ACGCTCACTA TGAACCGAAT GACTGTAGTA
    CAGGCATTTG TTGGTGGTAC TCATTATAGG CAGATTCCTG ATCCGGAGGC ACTGAACACA
    AAAATTCTTG ATCTTATAGT AAATGGGATT TCAGTCAACT CAGCATACAC CTCCAAGATC
    CTGCCCCCTG AAAAGGAGGG AGGTCTCCCC AGGCAGGTGG GTAACAAGAC AGAATGTGCG
    CTACTGGGAT TTGTTTTGGA CCTGAAGCAA GATTATCAGA CAGTGCGAAA CGAAATTCCT
    GAAGAGAACC TCTACAAAGT TTATACGTTC AATTCTGTGC GAAAATCTAT GAGCACAGTG
    TTGTGTGACT CTTCTGGAAA ATTCCGCATG TACAGCAAAG GGGCCTCTGA AATCATCTTG
    CGCAAATGCA CCCGAATCCT GGACCAAGGA GGAGATGTGT GCCCCTTTAA AGCAAAGGAC
    CGGGATGAGA TGGTAAAGAA AGTAATTGAA CCTATGGCAT GCGATGGCTT GCGCACAATC
    TGCCTAGCAT ATCGTGACTT CCCTTCAGAT AGTGAGCCTA ATTGGGATAA TGAGGGCGAC
    ATCCTCTCTG ACCTTATTTG CATTGCTGTA GTAGGAATAG AAGATCCTGT TCGACCAGAG
    GTGCCTGAAG CAATTCAAAA GTGCCAGCGT GCAGGTATCA CAGTGCGCAT GGTTACTGGG
    GACAATATAA ACACAGCTCG AGCAATAGCT ACCAAGTGTG GTATCCTGCA GCCAGGAGAA
    GACTTCTTGT GCTTGGAGGG AAAAGAATTC AACACACTGA TCCGAAATGA AAAGGGAGAG
    GTAGAACAGG ATAAATTGGA CAAGGTTTGG CCGAGACTTC GTGTTTTGGC CCGGTCATCA
    CCTACAGATA AACATACTCT GGTAAAAGGC ATCATTGACA GCACAGTGGC AGAGCGAAGA
    CAGGTGGTGG CTGTGACTGG AGATGGAACA AACGATGGAC CTGCCCTGAA GAAAGCAGAT
    GTGGGATTTG CAATGGGCAT AGCTGGCACA GATGTTGCAA AGGAGGCTTC TGATATTATC
    TTGACAGACG ACAATTTCAC CAGCATTGTA AAGGCTGTAA TGTGGGGCCG CAATGTATAC
    GACAGTATTT CGAAGTTCCT GCAGTTCCAG CTCACCGTAA ATGTTGTGGC AGTAATTGTG
    GCATTTACAG GAGCCTGCAT AACTCAGGAT TCACCACTGA AAGCTGTGCA GATGCTGTGG
    GTCAACCTTA TCATGGATAC ATTTGCATCT TTAGCTTTGG CGACTGAGCC TCCTACTGAT
    TCGCTTCTTC TACGCAAACC ATATGGGCGC AACAAACCCC TGATCTCACG TACAATGATG
    AAAAACATAT TGGGACATGC TGTTTATCAG CTTACCATCA TATTTACATT GCTTTTTGCA
    GGTGAGAAGT TTTTTGACAT TGACAGTGGG AGAAATGTAC CCCTGCATTC TCCACCTTCT
    GAACATTATA CAATTGTCTT CAACACCTTT GTGATGATGC AGCTTTTTAA TGAGATCAAT
    GCCCGCAAGA TCCACGGTGA ACGTAATGTC TTTGAGAACA TCTTCCGGAA CCCCATCTTT
    TGTGCAGTTG TGCTGGGCAC TTTTGGAGCT CAGATAATAA TTGTGGAGTT TGGAGGAAAG
    CCATTCAGTT GCTCTGGCCT TACTTTGTCA CAGTGGTTCT GGTGTATCTT CATTGGGGTT
    GGAGAGCTGT TGTGGGGACA GATGATTTGC ACAATACCCA CCAGCCGACT CAAGTTCCTA
    AAAGAGGCTG GCCATGGGCC ACTGAAGGAG GACATTCAGC AGGAGGAACT TACAGAGGAT
    GTAGATGAGA TTGACCATGC AGAAATGGAG CTTAGGAGAG GCCAGATTTT GTGGTTCAGA
    GGCCTAAACC GAATACAGAC TCAGATGGAC GTAGTTTATA CATTCCAGAC GGGAGGTACC
    TCTTTACAAG GGGCTCTGAG GCGCCAGCCT TCCACCATTA GCCAGCACCT TGATGCTAAA
    CATGCCTCTA GCCCAACACA CGTAGCTCTG TCCTCTACTA CCTCTCCCCC AGCCACTTCT
    GCTGCTGCTG CTTCTGTGGG CAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa42725
    Clone ID Related Accession (Same CDS sequence) XM_018091531.1 , XM_018091531.2
    Accession Version XM_018091531.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3582bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product plasma membrane calcium-transporting ATPase 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCAAATA ACTCCACAGA CCATCATTCT GCTAACATTG TAAATGAAGG CAGCTATGAA 
    GGAGATTTTG GCTGCACAAT AATGGACTTA CGGGAGCTTA TGGAATTACG CAGTGCAGAG
    GCAGTCAACC GTATCAGGGA TACTTATGGA GGGGTTCACA ACATCTGCCG CAGGCTAAAG
    ACTTCACCTG TAGAAGGTTT GTCAGGTAAC CCCTCAGACC TCGAGAGGAG GAGACAAATA
    TTTGGTAAAA ACTTCATCCC TCCAAAAAAA GCCAAAACTT TCTTGCAGCT GGTGTGGGAA
    GCACTGCAGG ACGTTACCTT AATTATCCTG GAAATAGCAG CCATAATATC TCTGGGTCTT
    TCTTTCTACC ACCCACCAGG AGGAGATAAT GAATTATGTG GGGAAGCTGC TGCAGGAGTG
    GAGGATGAAG GTGAGGCTCA AGCTGGATGG ATTGAAGGGG CAGCCATCTT ATTCTCGGTC
    ATCATAGTTG TGCTTGTTAC CGCCTTCAAT GACTGGAGCA AAGAGAAGCA ATTTCGAGGC
    CTACAGAGCC GAATTGAGCA GGAGCAGAAA TTCACTGTTA TCCGCAAAGG ACAGGTTATC
    CAAATCCCTG TAGCAGAGCT AGTAGTCGGT GATATTGCAC AAATAAAATA TGGTGACCTC
    CTTCCTGCTG ATGGGATATT GATTCAAGGC AATGACTTAA AAATTGATGA AAGCTCTCTA
    ACTGGGGAGA GCGATCAGGT GAAAAAATCA TTAGAGAAAG ACCCCATGCT TTTGTCAGGA
    ACACACGTAA TGGAAGGGTC TGGACGTATG GTAGTCACAG CAGTAGGTGT CAACTCTCAA
    ACTGGCATAA TATTCACTTT GCTAGGTGCA AATGAGGGAG GTGAAGAGGA AAAAAAATCC
    AAAAAAGCCA AAGCTCAAGA TGGTGTTGCA TTAGAGATCC AACCACTGAA AAGTGAAGAG
    GGCGTAGAGT GTGAAGAGAA AGAGAAAAAG GTTGTGAAAG TGCCAAAGAA AGAAAAGTCT
    GTCCTTCAGG GGAAGCTAAC TCGGCTGGCA GTGCAAATTG GAAAAGCAGG TCTCATTATG
    TCAGCCATCA CAGTGATCAT TTTGGTGCTC TATTTTGTCA TTTATACATT TGGAGTGTTA
    GGTCGCCCAT GGTTGGCAGA ATGCACTCCC ATCTACATCC AGTACTTTGT CAAATTTTTC
    ATTATTGGTG TCACTGTGTT GGTGGTGGCT GTCCCTGAGG GGCTGCCTCT CGCTGTAACA
    ATATCTTTGG CCTACTCTGT AAAGAAAATG ATGAAAGACA ATAATCTTGT ACGTCACTTG
    GATGCCTGTG AAACTATGGG CAATGCAACA GCCATATGCT CTGATAAAAC TGGAACGCTC
    ACTATGAACC GAATGACTGT AGTACAGGCA TTTGTTGGTG GTACTCATTA TAGGCAGATT
    CCTGATCCGG AGGCACTGAA CACAAAAATT CTTGATCTTA TAGTAAATGG GATTTCAGTC
    AACTCAGCAT ACACCTCCAA GATCCTGCCC CCTGAAAAGG AGGGAGGTCT CCCCAGGCAG
    GTGGGTAACA AGACAGAATG TGCGCTACTG GGATTTGTTT TGGACCTGAA GCAAGATTAT
    CAGACAGTGC GAAACGAAAT TCCTGAAGAG AACCTCTACA AAGTTTATAC GTTCAATTCT
    GTGCGAAAAT CTATGAGCAC AGTGTTGTGT GACTCTTCTG GAAAATTCCG CATGTACAGC
    AAAGGGGCCT CTGAAATCAT CTTGCGCAAA TGCACCCGAA TCCTGGACCA AGGAGGAGAT
    GTGTGCCCCT TTAAAGCAAA GGACCGGGAT GAGATGGTAA AGAAAGTAAT TGAACCTATG
    GCATGCGATG GCTTGCGCAC AATCTGCCTA GCATATCGTG ACTTCCCTTC AGATAGTGAG
    CCTAATTGGG ATAATGAGGG CGACATCCTC TCTGACCTTA TTTGCATTGC TGTAGTAGGA
    ATAGAAGATC CTGTTCGACC AGAGGTGCCT GAAGCAATTC AAAAGTGCCA GCGTGCAGGT
    ATCACAGTGC GCATGGTTAC TGGGGACAAT ATAAACACAG CTCGAGCAAT AGCTACCAAG
    TGTGGTATCC TGCAGCCAGG AGAAGACTTC TTGTGCTTGG AGGGAAAAGA ATTCAACACA
    CTGATCCGAA ATGAAAAGGG AGAGGTAGAA CAGGATAAAT TGGACAAGGT TTGGCCGAGA
    CTTCGTGTTT TGGCCCGGTC ATCACCTACA GATAAACATA CTCTGGTAAA AGGCATCATT
    GACAGCACAG TGGCAGAGCG AAGACAGGTG GTGGCTGTGA CTGGAGATGG AACAAACGAT
    GGACCTGCCC TGAAGAAAGC AGATGTGGGA TTTGCAATGG GCATAGCTGG CACAGATGTT
    GCAAAGGAGG CTTCTGATAT TATCTTGACA GACGACAATT TCACCAGCAT TGTAAAGGCT
    GTAATGTGGG GCCGCAATGT ATACGACAGT ATTTCGAAGT TCCTGCAGTT CCAGCTCACC
    GTAAATGTTG TGGCAGTAAT TGTGGCATTT ACAGGAGCCT GCATAACTCA GGATTCACCA
    CTGAAAGCTG TGCAGATGCT GTGGGTCAAC CTTATCATGG ATACATTTGC ATCTTTAGCT
    TTGGCGACTG AGCCTCCTAC TGATTCGCTT CTTCTACGCA AACCATATGG GCGCAACAAA
    CCCCTGATCT CACGTACAAT GATGAAAAAC ATATTGGGAC ATGCTGTTTA TCAGCTTACC
    ATCATATTTA CATTGCTTTT TGCAGGTGAG AAGTTTTTTG ACATTGACAG TGGGAGAAAT
    GTACCCCTGC ATTCTCCACC TTCTGAACAT TATACAATTG TCTTCAACAC CTTTGTGATG
    ATGCAGCTTT TTAATGAGAT CAATGCCCGC AAGATCCACG GTGAACGTAA TGTCTTTGAG
    AACATCTTCC GGAACCCCAT CTTTTGTGCA GTTGTGCTGG GCACTTTTGG AGCTCAGATA
    ATAATTGTGG AGTTTGGAGG AAAGCCATTC AGTTGCTCTG GCCTTACTTT GTCACAGTGG
    TTCTGGTGTA TCTTCATTGG GGTTGGAGAG CTGTTGTGGG GACAGATGAT TTGCACAATA
    CCCACCAGCC GACTCAAGTT CCTAAAAGAG GCTGGCCATG GGCCACTGAA GGAGGACATT
    CAGCAGGAGG AACTTACAGA GGATGTAGAT GAGATTGACC ATGCAGAAAT GGAGCTTAGG
    AGAGGCCAGA TTTTGTGGTT CAGAGGCCTA AACCGAATAC AGACTCAGAT TAAAGTTGTG
    AATGCGTTCC GAAGCTCTCT CTACGAGGGG CTAGAGAAGC CACAGATCCG CAGTGCCATC
    CATAGCTTCA TGACCCACCC AGAGTTCACT CCACGGGAGG CAGGACCTGC CCCCCAATTC
    GAAGACAGCG ACAGCGAGGG TGAGGAATCT AAGGGTCTCT CAAAGGCGCA TAAAGATCAA
    ACCCATAATC ACAACAACAA TTCTTCTGTT GACCCACATG CATTGGATAT CACAGTGTCT
    CTATCTGATA GCCCATTGCA TAGCATGGAA ACCTCCATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947020.1
    CDS693..4274
    Translation

    Target ORF information:

    RefSeq Version XM_018091531.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis plasma membrane calcium-transporting ATPase 4 (LOC100488346), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091531.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCAAATA ACTCCACAGA CCATCATTCT GCTAACATTG TAAATGAAGG CAGCTATGAA 
    GGAGATTTTG GCTGCACAAT AATGGACTTA CGGGAGCTTA TGGAATTACG CAGTGCAGAG
    GCAGTCAACC GTATCAGGGA TACTTATGGA GGGGTTCACA ACATCTGCCG CAGGCTAAAG
    ACTTCACCTG TAGAAGGTTT GTCAGGTAAC CCCTCAGACC TCGAGAGGAG GAGACAAATA
    TTTGGTAAAA ACTTCATCCC TCCAAAAAAA GCCAAAACTT TCTTGCAGCT GGTGTGGGAA
    GCACTGCAGG ACGTTACCTT AATTATCCTG GAAATAGCAG CCATAATATC TCTGGGTCTT
    TCTTTCTACC ACCCACCAGG AGGAGATAAT GAATTATGTG GGGAAGCTGC TGCAGGAGTG
    GAGGATGAAG GTGAGGCTCA AGCTGGATGG ATTGAAGGGG CAGCCATCTT ATTCTCGGTC
    ATCATAGTTG TGCTTGTTAC CGCCTTCAAT GACTGGAGCA AAGAGAAGCA ATTTCGAGGC
    CTACAGAGCC GAATTGAGCA GGAGCAGAAA TTCACTGTTA TCCGCAAAGG ACAGGTTATC
    CAAATCCCTG TAGCAGAGCT AGTAGTCGGT GATATTGCAC AAATAAAATA TGGTGACCTC
    CTTCCTGCTG ATGGGATATT GATTCAAGGC AATGACTTAA AAATTGATGA AAGCTCTCTA
    ACTGGGGAGA GCGATCAGGT GAAAAAATCA TTAGAGAAAG ACCCCATGCT TTTGTCAGGA
    ACACACGTAA TGGAAGGGTC TGGACGTATG GTAGTCACAG CAGTAGGTGT CAACTCTCAA
    ACTGGCATAA TATTCACTTT GCTAGGTGCA AATGAGGGAG GTGAAGAGGA AAAAAAATCC
    AAAAAAGCCA AAGCTCAAGA TGGTGTTGCA TTAGAGATCC AACCACTGAA AAGTGAAGAG
    GGCGTAGAGT GTGAAGAGAA AGAGAAAAAG GTTGTGAAAG TGCCAAAGAA AGAAAAGTCT
    GTCCTTCAGG GGAAGCTAAC TCGGCTGGCA GTGCAAATTG GAAAAGCAGG TCTCATTATG
    TCAGCCATCA CAGTGATCAT TTTGGTGCTC TATTTTGTCA TTTATACATT TGGAGTGTTA
    GGTCGCCCAT GGTTGGCAGA ATGCACTCCC ATCTACATCC AGTACTTTGT CAAATTTTTC
    ATTATTGGTG TCACTGTGTT GGTGGTGGCT GTCCCTGAGG GGCTGCCTCT CGCTGTAACA
    ATATCTTTGG CCTACTCTGT AAAGAAAATG ATGAAAGACA ATAATCTTGT ACGTCACTTG
    GATGCCTGTG AAACTATGGG CAATGCAACA GCCATATGCT CTGATAAAAC TGGAACGCTC
    ACTATGAACC GAATGACTGT AGTACAGGCA TTTGTTGGTG GTACTCATTA TAGGCAGATT
    CCTGATCCGG AGGCACTGAA CACAAAAATT CTTGATCTTA TAGTAAATGG GATTTCAGTC
    AACTCAGCAT ACACCTCCAA GATCCTGCCC CCTGAAAAGG AGGGAGGTCT CCCCAGGCAG
    GTGGGTAACA AGACAGAATG TGCGCTACTG GGATTTGTTT TGGACCTGAA GCAAGATTAT
    CAGACAGTGC GAAACGAAAT TCCTGAAGAG AACCTCTACA AAGTTTATAC GTTCAATTCT
    GTGCGAAAAT CTATGAGCAC AGTGTTGTGT GACTCTTCTG GAAAATTCCG CATGTACAGC
    AAAGGGGCCT CTGAAATCAT CTTGCGCAAA TGCACCCGAA TCCTGGACCA AGGAGGAGAT
    GTGTGCCCCT TTAAAGCAAA GGACCGGGAT GAGATGGTAA AGAAAGTAAT TGAACCTATG
    GCATGCGATG GCTTGCGCAC AATCTGCCTA GCATATCGTG ACTTCCCTTC AGATAGTGAG
    CCTAATTGGG ATAATGAGGG CGACATCCTC TCTGACCTTA TTTGCATTGC TGTAGTAGGA
    ATAGAAGATC CTGTTCGACC AGAGGTGCCT GAAGCAATTC AAAAGTGCCA GCGTGCAGGT
    ATCACAGTGC GCATGGTTAC TGGGGACAAT ATAAACACAG CTCGAGCAAT AGCTACCAAG
    TGTGGTATCC TGCAGCCAGG AGAAGACTTC TTGTGCTTGG AGGGAAAAGA ATTCAACACA
    CTGATCCGAA ATGAAAAGGG AGAGGTAGAA CAGGATAAAT TGGACAAGGT TTGGCCGAGA
    CTTCGTGTTT TGGCCCGGTC ATCACCTACA GATAAACATA CTCTGGTAAA AGGCATCATT
    GACAGCACAG TGGCAGAGCG AAGACAGGTG GTGGCTGTGA CTGGAGATGG AACAAACGAT
    GGACCTGCCC TGAAGAAAGC AGATGTGGGA TTTGCAATGG GCATAGCTGG CACAGATGTT
    GCAAAGGAGG CTTCTGATAT TATCTTGACA GACGACAATT TCACCAGCAT TGTAAAGGCT
    GTAATGTGGG GCCGCAATGT ATACGACAGT ATTTCGAAGT TCCTGCAGTT CCAGCTCACC
    GTAAATGTTG TGGCAGTAAT TGTGGCATTT ACAGGAGCCT GCATAACTCA GGATTCACCA
    CTGAAAGCTG TGCAGATGCT GTGGGTCAAC CTTATCATGG ATACATTTGC ATCTTTAGCT
    TTGGCGACTG AGCCTCCTAC TGATTCGCTT CTTCTACGCA AACCATATGG GCGCAACAAA
    CCCCTGATCT CACGTACAAT GATGAAAAAC ATATTGGGAC ATGCTGTTTA TCAGCTTACC
    ATCATATTTA CATTGCTTTT TGCAGGTGAG AAGTTTTTTG ACATTGACAG TGGGAGAAAT
    GTACCCCTGC ATTCTCCACC TTCTGAACAT TATACAATTG TCTTCAACAC CTTTGTGATG
    ATGCAGCTTT TTAATGAGAT CAATGCCCGC AAGATCCACG GTGAACGTAA TGTCTTTGAG
    AACATCTTCC GGAACCCCAT CTTTTGTGCA GTTGTGCTGG GCACTTTTGG AGCTCAGATA
    ATAATTGTGG AGTTTGGAGG AAAGCCATTC AGTTGCTCTG GCCTTACTTT GTCACAGTGG
    TTCTGGTGTA TCTTCATTGG GGTTGGAGAG CTGTTGTGGG GACAGATGAT TTGCACAATA
    CCCACCAGCC GACTCAAGTT CCTAAAAGAG GCTGGCCATG GGCCACTGAA GGAGGACATT
    CAGCAGGAGG AACTTACAGA GGATGTAGAT GAGATTGACC ATGCAGAAAT GGAGCTTAGG
    AGAGGCCAGA TTTTGTGGTT CAGAGGCCTA AACCGAATAC AGACTCAGAT TAAAGTTGTG
    AATGCGTTCC GAAGCTCTCT CTACGAGGGG CTAGAGAAGC CACAGATCCG CAGTGCCATC
    CATAGCTTCA TGACCCACCC AGAGTTCACT CCACGGGAGG CAGGACCTGC CCCCCAATTC
    GAAGACAGCG ACAGCGAGGG TGAGGAATCT AAGGGTCTCT CAAAGGCGCA TAAAGATCAA
    ACCCATAATC ACAACAACAA TTCTTCTGTT GACCCACATG CATTGGATAT CACAGTGTCT
    CTATCTGATA GCCCATTGCA TAGCATGGAA ACCTCCATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa42725
    Clone ID Related Accession (Same CDS sequence) XM_018091531.1 , XM_018091531.2
    Accession Version XM_018091531.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3582bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product plasma membrane calcium-transporting ATPase 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018091531.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCAAATA ACTCCACAGA CCATCATTCT GCTAACATTG TAAATGAAGG CAGCTATGAA 
    GGAGATTTTG GCTGCACAAT AATGGACTTA CGGGAGCTTA TGGAATTACG CAGTGCAGAG
    GCAGTCAACC GTATCAGGGA TACTTATGGA GGGGTTCACA ACATCTGCCG CAGGCTAAAG
    ACTTCACCTG TAGAAGGTTT GTCAGGTAAC CCCTCAGACC TCGAGAGGAG GAGACAAATA
    TTTGGTAAAA ACTTCATCCC TCCAAAAAAA GCCAAAACTT TCTTGCAGCT GGTGTGGGAA
    GCACTGCAGG ACGTTACCTT AATTATCCTG GAAATAGCAG CCATAATATC TCTGGGTCTT
    TCTTTCTACC ACCCACCAGG AGGAGATAAT GAATTATGTG GGGAAGCTGC TGCAGGAGTG
    GAGGATGAAG GTGAGGCTCA AGCTGGATGG ATTGAAGGGG CAGCCATCTT ATTCTCGGTC
    ATCATAGTTG TGCTTGTTAC CGCCTTCAAT GACTGGAGCA AAGAGAAGCA ATTTCGAGGC
    CTACAGAGCC GAATTGAGCA GGAGCAGAAA TTCACTGTTA TCCGCAAAGG ACAGGTTATC
    CAAATCCCTG TAGCAGAGCT AGTAGTCGGT GATATTGCAC AAATAAAATA TGGTGACCTC
    CTTCCTGCTG ATGGGATATT GATTCAAGGC AATGACTTAA AAATTGATGA AAGCTCTCTA
    ACTGGGGAGA GCGATCAGGT GAAAAAATCA TTAGAGAAAG ACCCCATGCT TTTGTCAGGA
    ACACACGTAA TGGAAGGGTC TGGACGTATG GTAGTCACAG CAGTAGGTGT CAACTCTCAA
    ACTGGCATAA TATTCACTTT GCTAGGTGCA AATGAGGGAG GTGAAGAGGA AAAAAAATCC
    AAAAAAGCCA AAGCTCAAGA TGGTGTTGCA TTAGAGATCC AACCACTGAA AAGTGAAGAG
    GGCGTAGAGT GTGAAGAGAA AGAGAAAAAG GTTGTGAAAG TGCCAAAGAA AGAAAAGTCT
    GTCCTTCAGG GGAAGCTAAC TCGGCTGGCA GTGCAAATTG GAAAAGCAGG TCTCATTATG
    TCAGCCATCA CAGTGATCAT TTTGGTGCTC TATTTTGTCA TTTATACATT TGGAGTGTTA
    GGTCGCCCAT GGTTGGCAGA ATGCACTCCC ATCTACATCC AGTACTTTGT CAAATTTTTC
    ATTATTGGTG TCACTGTGTT GGTGGTGGCT GTCCCTGAGG GGCTGCCTCT CGCTGTAACA
    ATATCTTTGG CCTACTCTGT AAAGAAAATG ATGAAAGACA ATAATCTTGT ACGTCACTTG
    GATGCCTGTG AAACTATGGG CAATGCAACA GCCATATGCT CTGATAAAAC TGGAACGCTC
    ACTATGAACC GAATGACTGT AGTACAGGCA TTTGTTGGTG GTACTCATTA TAGGCAGATT
    CCTGATCCGG AGGCACTGAA CACAAAAATT CTTGATCTTA TAGTAAATGG GATTTCAGTC
    AACTCAGCAT ACACCTCCAA GATCCTGCCC CCTGAAAAGG AGGGAGGTCT CCCCAGGCAG
    GTGGGTAACA AGACAGAATG TGCGCTACTG GGATTTGTTT TGGACCTGAA GCAAGATTAT
    CAGACAGTGC GAAACGAAAT TCCTGAAGAG AACCTCTACA AAGTTTATAC GTTCAATTCT
    GTGCGAAAAT CTATGAGCAC AGTGTTGTGT GACTCTTCTG GAAAATTCCG CATGTACAGC
    AAAGGGGCCT CTGAAATCAT CTTGCGCAAA TGCACCCGAA TCCTGGACCA AGGAGGAGAT
    GTGTGCCCCT TTAAAGCAAA GGACCGGGAT GAGATGGTAA AGAAAGTAAT TGAACCTATG
    GCATGCGATG GCTTGCGCAC AATCTGCCTA GCATATCGTG ACTTCCCTTC AGATAGTGAG
    CCTAATTGGG ATAATGAGGG CGACATCCTC TCTGACCTTA TTTGCATTGC TGTAGTAGGA
    ATAGAAGATC CTGTTCGACC AGAGGTGCCT GAAGCAATTC AAAAGTGCCA GCGTGCAGGT
    ATCACAGTGC GCATGGTTAC TGGGGACAAT ATAAACACAG CTCGAGCAAT AGCTACCAAG
    TGTGGTATCC TGCAGCCAGG AGAAGACTTC TTGTGCTTGG AGGGAAAAGA ATTCAACACA
    CTGATCCGAA ATGAAAAGGG AGAGGTAGAA CAGGATAAAT TGGACAAGGT TTGGCCGAGA
    CTTCGTGTTT TGGCCCGGTC ATCACCTACA GATAAACATA CTCTGGTAAA AGGCATCATT
    GACAGCACAG TGGCAGAGCG AAGACAGGTG GTGGCTGTGA CTGGAGATGG AACAAACGAT
    GGACCTGCCC TGAAGAAAGC AGATGTGGGA TTTGCAATGG GCATAGCTGG CACAGATGTT
    GCAAAGGAGG CTTCTGATAT TATCTTGACA GACGACAATT TCACCAGCAT TGTAAAGGCT
    GTAATGTGGG GCCGCAATGT ATACGACAGT ATTTCGAAGT TCCTGCAGTT CCAGCTCACC
    GTAAATGTTG TGGCAGTAAT TGTGGCATTT ACAGGAGCCT GCATAACTCA GGATTCACCA
    CTGAAAGCTG TGCAGATGCT GTGGGTCAAC CTTATCATGG ATACATTTGC ATCTTTAGCT
    TTGGCGACTG AGCCTCCTAC TGATTCGCTT CTTCTACGCA AACCATATGG GCGCAACAAA
    CCCCTGATCT CACGTACAAT GATGAAAAAC ATATTGGGAC ATGCTGTTTA TCAGCTTACC
    ATCATATTTA CATTGCTTTT TGCAGGTGAG AAGTTTTTTG ACATTGACAG TGGGAGAAAT
    GTACCCCTGC ATTCTCCACC TTCTGAACAT TATACAATTG TCTTCAACAC CTTTGTGATG
    ATGCAGCTTT TTAATGAGAT CAATGCCCGC AAGATCCACG GTGAACGTAA TGTCTTTGAG
    AACATCTTCC GGAACCCCAT CTTTTGTGCA GTTGTGCTGG GCACTTTTGG AGCTCAGATA
    ATAATTGTGG AGTTTGGAGG AAAGCCATTC AGTTGCTCTG GCCTTACTTT GTCACAGTGG
    TTCTGGTGTA TCTTCATTGG GGTTGGAGAG CTGTTGTGGG GACAGATGAT TTGCACAATA
    CCCACCAGCC GACTCAAGTT CCTAAAAGAG GCTGGCCATG GGCCACTGAA GGAGGACATT
    CAGCAGGAGG AACTTACAGA GGATGTAGAT GAGATTGACC ATGCAGAAAT GGAGCTTAGG
    AGAGGCCAGA TTTTGTGGTT CAGAGGCCTA AACCGAATAC AGACTCAGAT TAAAGTTGTG
    AATGCGTTCC GAAGCTCTCT CTACGAGGGG CTAGAGAAGC CACAGATCCG CAGTGCCATC
    CATAGCTTCA TGACCCACCC AGAGTTCACT CCACGGGAGG CAGGACCTGC CCCCCAATTC
    GAAGACAGCG ACAGCGAGGG TGAGGAATCT AAGGGTCTCT CAAAGGCGCA TAAAGATCAA
    ACCCATAATC ACAACAACAA TTCTTCTGTT GACCCACATG CATTGGATAT CACAGTGTCT
    CTATCTGATA GCCCATTGCA TAGCATGGAA ACCTCCATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947020.1
    CDS664..4245
    Translation

    Target ORF information:

    RefSeq Version XM_018091531.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis plasma membrane calcium-transporting ATPase 4 (LOC100488346), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091531.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCAAATA ACTCCACAGA CCATCATTCT GCTAACATTG TAAATGAAGG CAGCTATGAA 
    GGAGATTTTG GCTGCACAAT AATGGACTTA CGGGAGCTTA TGGAATTACG CAGTGCAGAG
    GCAGTCAACC GTATCAGGGA TACTTATGGA GGGGTTCACA ACATCTGCCG CAGGCTAAAG
    ACTTCACCTG TAGAAGGTTT GTCAGGTAAC CCCTCAGACC TCGAGAGGAG GAGACAAATA
    TTTGGTAAAA ACTTCATCCC TCCAAAAAAA GCCAAAACTT TCTTGCAGCT GGTGTGGGAA
    GCACTGCAGG ACGTTACCTT AATTATCCTG GAAATAGCAG CCATAATATC TCTGGGTCTT
    TCTTTCTACC ACCCACCAGG AGGAGATAAT GAATTATGTG GGGAAGCTGC TGCAGGAGTG
    GAGGATGAAG GTGAGGCTCA AGCTGGATGG ATTGAAGGGG CAGCCATCTT ATTCTCGGTC
    ATCATAGTTG TGCTTGTTAC CGCCTTCAAT GACTGGAGCA AAGAGAAGCA ATTTCGAGGC
    CTACAGAGCC GAATTGAGCA GGAGCAGAAA TTCACTGTTA TCCGCAAAGG ACAGGTTATC
    CAAATCCCTG TAGCAGAGCT AGTAGTCGGT GATATTGCAC AAATAAAATA TGGTGACCTC
    CTTCCTGCTG ATGGGATATT GATTCAAGGC AATGACTTAA AAATTGATGA AAGCTCTCTA
    ACTGGGGAGA GCGATCAGGT GAAAAAATCA TTAGAGAAAG ACCCCATGCT TTTGTCAGGA
    ACACACGTAA TGGAAGGGTC TGGACGTATG GTAGTCACAG CAGTAGGTGT CAACTCTCAA
    ACTGGCATAA TATTCACTTT GCTAGGTGCA AATGAGGGAG GTGAAGAGGA AAAAAAATCC
    AAAAAAGCCA AAGCTCAAGA TGGTGTTGCA TTAGAGATCC AACCACTGAA AAGTGAAGAG
    GGCGTAGAGT GTGAAGAGAA AGAGAAAAAG GTTGTGAAAG TGCCAAAGAA AGAAAAGTCT
    GTCCTTCAGG GGAAGCTAAC TCGGCTGGCA GTGCAAATTG GAAAAGCAGG TCTCATTATG
    TCAGCCATCA CAGTGATCAT TTTGGTGCTC TATTTTGTCA TTTATACATT TGGAGTGTTA
    GGTCGCCCAT GGTTGGCAGA ATGCACTCCC ATCTACATCC AGTACTTTGT CAAATTTTTC
    ATTATTGGTG TCACTGTGTT GGTGGTGGCT GTCCCTGAGG GGCTGCCTCT CGCTGTAACA
    ATATCTTTGG CCTACTCTGT AAAGAAAATG ATGAAAGACA ATAATCTTGT ACGTCACTTG
    GATGCCTGTG AAACTATGGG CAATGCAACA GCCATATGCT CTGATAAAAC TGGAACGCTC
    ACTATGAACC GAATGACTGT AGTACAGGCA TTTGTTGGTG GTACTCATTA TAGGCAGATT
    CCTGATCCGG AGGCACTGAA CACAAAAATT CTTGATCTTA TAGTAAATGG GATTTCAGTC
    AACTCAGCAT ACACCTCCAA GATCCTGCCC CCTGAAAAGG AGGGAGGTCT CCCCAGGCAG
    GTGGGTAACA AGACAGAATG TGCGCTACTG GGATTTGTTT TGGACCTGAA GCAAGATTAT
    CAGACAGTGC GAAACGAAAT TCCTGAAGAG AACCTCTACA AAGTTTATAC GTTCAATTCT
    GTGCGAAAAT CTATGAGCAC AGTGTTGTGT GACTCTTCTG GAAAATTCCG CATGTACAGC
    AAAGGGGCCT CTGAAATCAT CTTGCGCAAA TGCACCCGAA TCCTGGACCA AGGAGGAGAT
    GTGTGCCCCT TTAAAGCAAA GGACCGGGAT GAGATGGTAA AGAAAGTAAT TGAACCTATG
    GCATGCGATG GCTTGCGCAC AATCTGCCTA GCATATCGTG ACTTCCCTTC AGATAGTGAG
    CCTAATTGGG ATAATGAGGG CGACATCCTC TCTGACCTTA TTTGCATTGC TGTAGTAGGA
    ATAGAAGATC CTGTTCGACC AGAGGTGCCT GAAGCAATTC AAAAGTGCCA GCGTGCAGGT
    ATCACAGTGC GCATGGTTAC TGGGGACAAT ATAAACACAG CTCGAGCAAT AGCTACCAAG
    TGTGGTATCC TGCAGCCAGG AGAAGACTTC TTGTGCTTGG AGGGAAAAGA ATTCAACACA
    CTGATCCGAA ATGAAAAGGG AGAGGTAGAA CAGGATAAAT TGGACAAGGT TTGGCCGAGA
    CTTCGTGTTT TGGCCCGGTC ATCACCTACA GATAAACATA CTCTGGTAAA AGGCATCATT
    GACAGCACAG TGGCAGAGCG AAGACAGGTG GTGGCTGTGA CTGGAGATGG AACAAACGAT
    GGACCTGCCC TGAAGAAAGC AGATGTGGGA TTTGCAATGG GCATAGCTGG CACAGATGTT
    GCAAAGGAGG CTTCTGATAT TATCTTGACA GACGACAATT TCACCAGCAT TGTAAAGGCT
    GTAATGTGGG GCCGCAATGT ATACGACAGT ATTTCGAAGT TCCTGCAGTT CCAGCTCACC
    GTAAATGTTG TGGCAGTAAT TGTGGCATTT ACAGGAGCCT GCATAACTCA GGATTCACCA
    CTGAAAGCTG TGCAGATGCT GTGGGTCAAC CTTATCATGG ATACATTTGC ATCTTTAGCT
    TTGGCGACTG AGCCTCCTAC TGATTCGCTT CTTCTACGCA AACCATATGG GCGCAACAAA
    CCCCTGATCT CACGTACAAT GATGAAAAAC ATATTGGGAC ATGCTGTTTA TCAGCTTACC
    ATCATATTTA CATTGCTTTT TGCAGGTGAG AAGTTTTTTG ACATTGACAG TGGGAGAAAT
    GTACCCCTGC ATTCTCCACC TTCTGAACAT TATACAATTG TCTTCAACAC CTTTGTGATG
    ATGCAGCTTT TTAATGAGAT CAATGCCCGC AAGATCCACG GTGAACGTAA TGTCTTTGAG
    AACATCTTCC GGAACCCCAT CTTTTGTGCA GTTGTGCTGG GCACTTTTGG AGCTCAGATA
    ATAATTGTGG AGTTTGGAGG AAAGCCATTC AGTTGCTCTG GCCTTACTTT GTCACAGTGG
    TTCTGGTGTA TCTTCATTGG GGTTGGAGAG CTGTTGTGGG GACAGATGAT TTGCACAATA
    CCCACCAGCC GACTCAAGTT CCTAAAAGAG GCTGGCCATG GGCCACTGAA GGAGGACATT
    CAGCAGGAGG AACTTACAGA GGATGTAGAT GAGATTGACC ATGCAGAAAT GGAGCTTAGG
    AGAGGCCAGA TTTTGTGGTT CAGAGGCCTA AACCGAATAC AGACTCAGAT TAAAGTTGTG
    AATGCGTTCC GAAGCTCTCT CTACGAGGGG CTAGAGAAGC CACAGATCCG CAGTGCCATC
    CATAGCTTCA TGACCCACCC AGAGTTCACT CCACGGGAGG CAGGACCTGC CCCCCAATTC
    GAAGACAGCG ACAGCGAGGG TGAGGAATCT AAGGGTCTCT CAAAGGCGCA TAAAGATCAA
    ACCCATAATC ACAACAACAA TTCTTCTGTT GACCCACATG CATTGGATAT CACAGTGTCT
    CTATCTGATA GCCCATTGCA TAGCATGGAA ACCTCCATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa42726
    Clone ID Related Accession (Same CDS sequence) XM_018091532.2 , XM_018091532.1
    Accession Version XM_018091532.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3471bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product plasma membrane calcium-transporting ATPase 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018091532.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCAAATA ACTCCACAGA CCATCATTCT GCTAACATTG TAAATGAAGG CAGCTATGAA 
    GGAGATTTTG GCTGCACAAT AATGGACTTA CGGGAGCTTA TGGAATTACG CAGTGCAGAG
    GCAGTCAACC GTATCAGGGA TACTTATGGA GGGGTTCACA ACATCTGCCG CAGGCTAAAG
    ACTTCACCTG TAGAAGGTTT GTCAGGTAAC CCCTCAGACC TCGAGAGGAG GAGACAAATA
    TTTGGTAAAA ACTTCATCCC TCCAAAAAAA GCCAAAACTT TCTTGCAGCT GGTGTGGGAA
    GCACTGCAGG ACGTTACCTT AATTATCCTG GAAATAGCAG CCATAATATC TCTGGGTCTT
    TCTTTCTACC ACCCACCAGG AGGAGATAAT GAATTATGTG GGGAAGCTGC TGCAGGAGTG
    GAGGATGAAG GTGAGGCTCA AGCTGGATGG ATTGAAGGGG CAGCCATCTT ATTCTCGGTC
    ATCATAGTTG TGCTTGTTAC CGCCTTCAAT GACTGGAGCA AAGAGAAGCA ATTTCGAGGC
    CTACAGAGCC GAATTGAGCA GGAGCAGAAA TTCACTGTTA TCCGCAAAGG ACAGGTTATC
    CAAATCCCTG TAGCAGAGCT AGTAGTCGGT GATATTGCAC AAATAAAATA TGGTGACCTC
    CTTCCTGCTG ATGGGATATT GATTCAAGGC AATGACTTAA AAATTGATGA AAGCTCTCTA
    ACTGGGGAGA GCGATCAGGT GAAAAAATCA TTAGAGAAAG ACCCCATGCT TTTGTCAGGA
    ACACACGTAA TGGAAGGGTC TGGACGTATG GTAGTCACAG CAGTAGGTGT CAACTCTCAA
    ACTGGCATAA TATTCACTTT GCTAGGTGCA AATGAGGGAG GTGAAGAGGA AAAAAAATCC
    AAAAAAGCCA AAGCTCAAGA TGGTGTTGCA TTAGAGATCC AACCACTGAA AAGTGAAGAG
    GGCGTAGAGT GTGAAGAGAA AGAGAAAAAG GTTGTGAAAG TGCCAAAGAA AGAAAAGTCT
    GTCCTTCAGG GGAAGCTAAC TCGGCTGGCA GTGCAAATTG GAAAAGCAGG TCTCATTATG
    TCAGCCATCA CAGTGATCAT TTTGGTGCTC TATTTTGTCA TTTATACATT TGGAGTGTTA
    GGTCGCCCAT GGTTGGCAGA ATGCACTCCC ATCTACATCC AGTACTTTGT CAAATTTTTC
    ATTATTGGTG TCACTGTGTT GGTGGTGGCT GTCCCTGAGG GGCTGCCTCT CGCTGTAACA
    ATATCTTTGG CCTACTCTGT AAAGAAAATG ATGAAAGACA ATAATCTTGT ACGTCACTTG
    GATGCCTGTG AAACTATGGG CAATGCAACA GCCATATGCT CTGATAAAAC TGGAACGCTC
    ACTATGAACC GAATGACTGT AGTACAGGCA TTTGTTGGTG GTACTCATTA TAGGCAGATT
    CCTGATCCGG AGGCACTGAA CACAAAAATT CTTGATCTTA TAGTAAATGG GATTTCAGTC
    AACTCAGCAT ACACCTCCAA GATCCTGCCC CCTGAAAAGG AGGGAGGTCT CCCCAGGCAG
    GTGGGTAACA AGACAGAATG TGCGCTACTG GGATTTGTTT TGGACCTGAA GCAAGATTAT
    CAGACAGTGC GAAACGAAAT TCCTGAAGAG AACCTCTACA AAGTTTATAC GTTCAATTCT
    GTGCGAAAAT CTATGAGCAC AGTGTTGTGT GACTCTTCTG GAAAATTCCG CATGTACAGC
    AAAGGGGCCT CTGAAATCAT CTTGCGCAAA TGCACCCGAA TCCTGGACCA AGGAGGAGAT
    GTGTGCCCCT TTAAAGCAAA GGACCGGGAT GAGATGGTAA AGAAAGTAAT TGAACCTATG
    GCATGCGATG GCTTGCGCAC AATCTGCCTA GCATATCGTG ACTTCCCTTC AGATAGTGAG
    CCTAATTGGG ATAATGAGGG CGACATCCTC TCTGACCTTA TTTGCATTGC TGTAGTAGGA
    ATAGAAGATC CTGTTCGACC AGAGGTGCCT GAAGCAATTC AAAAGTGCCA GCGTGCAGGT
    ATCACAGTGC GCATGGTTAC TGGGGACAAT ATAAACACAG CTCGAGCAAT AGCTACCAAG
    TGTGGTATCC TGCAGCCAGG AGAAGACTTC TTGTGCTTGG AGGGAAAAGA ATTCAACACA
    CTGATCCGAA ATGAAAAGGG AGAGGTAGAA CAGGATAAAT TGGACAAGGT TTGGCCGAGA
    CTTCGTGTTT TGGCCCGGTC ATCACCTACA GATAAACATA CTCTGGTAAA AGGCATCATT
    GACAGCACAG TGGCAGAGCG AAGACAGGTG GTGGCTGTGA CTGGAGATGG AACAAACGAT
    GGACCTGCCC TGAAGAAAGC AGATGTGGGA TTTGCAATGG GCATAGCTGG CACAGATGTT
    GCAAAGGAGG CTTCTGATAT TATCTTGACA GACGACAATT TCACCAGCAT TGTAAAGGCT
    GTAATGTGGG GCCGCAATGT ATACGACAGT ATTTCGAAGT TCCTGCAGTT CCAGCTCACC
    GTAAATGTTG TGGCAGTAAT TGTGGCATTT ACAGGAGCCT GCATAACTCA GGATTCACCA
    CTGAAAGCTG TGCAGATGCT GTGGGTCAAC CTTATCATGG ATACATTTGC ATCTTTAGCT
    TTGGCGACTG AGCCTCCTAC TGATTCGCTT CTTCTACGCA AACCATATGG GCGCAACAAA
    CCCCTGATCT CACGTACAAT GATGAAAAAC ATATTGGGAC ATGCTGTTTA TCAGCTTACC
    ATCATATTTA CATTGCTTTT TGCAGGTGAG AAGTTTTTTG ACATTGACAG TGGGAGAAAT
    GTACCCCTGC ATTCTCCACC TTCTGAACAT TATACAATTG TCTTCAACAC CTTTGTGATG
    ATGCAGCTTT TTAATGAGAT CAATGCCCGC AAGATCCACG GTGAACGTAA TGTCTTTGAG
    AACATCTTCC GGAACCCCAT CTTTTGTGCA GTTGTGCTGG GCACTTTTGG AGCTCAGATA
    ATAATTGTGG AGTTTGGAGG AAAGCCATTC AGTTGCTCTG GCCTTACTTT GTCACAGTGG
    TTCTGGTGTA TCTTCATTGG GGTTGGAGAG CTGTTGTGGG GACAGATGAT TTGCACAATA
    CCCACCAGCC GACTCAAGTT CCTAAAAGAG GCTGGCCATG GGCCACTGAA GGAGGACATT
    CAGCAGGAGG AACTTACAGA GGATGTAGAT GAGATTGACC ATGCAGAAAT GGAGCTTAGG
    AGAGGCCAGA TTTTGTGGTT CAGAGGCCTA AACCGAATAC AGACTCAGAT GGACGTAGTT
    TATACATTCC AGACGGGAGG TACCTCTTTA CAAGGGGCTC TGAGGCGCCA GCCTTCCACC
    ATTAGCCAGC ACCTTGATGC TAAACATGCC TCTAGCCCAA CACACGTAGC TCTGTCCTCT
    ACTACCTCTC CCCCAGCCAC TTCTGCTGCT GCTGCTTCTG TGGGCAATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947021.1
    CDS666..4136
    Translation

    Target ORF information:

    RefSeq Version XM_018091532.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis plasma membrane calcium-transporting ATPase 4 (LOC100488346), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091532.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCAAATA ACTCCACAGA CCATCATTCT GCTAACATTG TAAATGAAGG CAGCTATGAA 
    GGAGATTTTG GCTGCACAAT AATGGACTTA CGGGAGCTTA TGGAATTACG CAGTGCAGAG
    GCAGTCAACC GTATCAGGGA TACTTATGGA GGGGTTCACA ACATCTGCCG CAGGCTAAAG
    ACTTCACCTG TAGAAGGTTT GTCAGGTAAC CCCTCAGACC TCGAGAGGAG GAGACAAATA
    TTTGGTAAAA ACTTCATCCC TCCAAAAAAA GCCAAAACTT TCTTGCAGCT GGTGTGGGAA
    GCACTGCAGG ACGTTACCTT AATTATCCTG GAAATAGCAG CCATAATATC TCTGGGTCTT
    TCTTTCTACC ACCCACCAGG AGGAGATAAT GAATTATGTG GGGAAGCTGC TGCAGGAGTG
    GAGGATGAAG GTGAGGCTCA AGCTGGATGG ATTGAAGGGG CAGCCATCTT ATTCTCGGTC
    ATCATAGTTG TGCTTGTTAC CGCCTTCAAT GACTGGAGCA AAGAGAAGCA ATTTCGAGGC
    CTACAGAGCC GAATTGAGCA GGAGCAGAAA TTCACTGTTA TCCGCAAAGG ACAGGTTATC
    CAAATCCCTG TAGCAGAGCT AGTAGTCGGT GATATTGCAC AAATAAAATA TGGTGACCTC
    CTTCCTGCTG ATGGGATATT GATTCAAGGC AATGACTTAA AAATTGATGA AAGCTCTCTA
    ACTGGGGAGA GCGATCAGGT GAAAAAATCA TTAGAGAAAG ACCCCATGCT TTTGTCAGGA
    ACACACGTAA TGGAAGGGTC TGGACGTATG GTAGTCACAG CAGTAGGTGT CAACTCTCAA
    ACTGGCATAA TATTCACTTT GCTAGGTGCA AATGAGGGAG GTGAAGAGGA AAAAAAATCC
    AAAAAAGCCA AAGCTCAAGA TGGTGTTGCA TTAGAGATCC AACCACTGAA AAGTGAAGAG
    GGCGTAGAGT GTGAAGAGAA AGAGAAAAAG GTTGTGAAAG TGCCAAAGAA AGAAAAGTCT
    GTCCTTCAGG GGAAGCTAAC TCGGCTGGCA GTGCAAATTG GAAAAGCAGG TCTCATTATG
    TCAGCCATCA CAGTGATCAT TTTGGTGCTC TATTTTGTCA TTTATACATT TGGAGTGTTA
    GGTCGCCCAT GGTTGGCAGA ATGCACTCCC ATCTACATCC AGTACTTTGT CAAATTTTTC
    ATTATTGGTG TCACTGTGTT GGTGGTGGCT GTCCCTGAGG GGCTGCCTCT CGCTGTAACA
    ATATCTTTGG CCTACTCTGT AAAGAAAATG ATGAAAGACA ATAATCTTGT ACGTCACTTG
    GATGCCTGTG AAACTATGGG CAATGCAACA GCCATATGCT CTGATAAAAC TGGAACGCTC
    ACTATGAACC GAATGACTGT AGTACAGGCA TTTGTTGGTG GTACTCATTA TAGGCAGATT
    CCTGATCCGG AGGCACTGAA CACAAAAATT CTTGATCTTA TAGTAAATGG GATTTCAGTC
    AACTCAGCAT ACACCTCCAA GATCCTGCCC CCTGAAAAGG AGGGAGGTCT CCCCAGGCAG
    GTGGGTAACA AGACAGAATG TGCGCTACTG GGATTTGTTT TGGACCTGAA GCAAGATTAT
    CAGACAGTGC GAAACGAAAT TCCTGAAGAG AACCTCTACA AAGTTTATAC GTTCAATTCT
    GTGCGAAAAT CTATGAGCAC AGTGTTGTGT GACTCTTCTG GAAAATTCCG CATGTACAGC
    AAAGGGGCCT CTGAAATCAT CTTGCGCAAA TGCACCCGAA TCCTGGACCA AGGAGGAGAT
    GTGTGCCCCT TTAAAGCAAA GGACCGGGAT GAGATGGTAA AGAAAGTAAT TGAACCTATG
    GCATGCGATG GCTTGCGCAC AATCTGCCTA GCATATCGTG ACTTCCCTTC AGATAGTGAG
    CCTAATTGGG ATAATGAGGG CGACATCCTC TCTGACCTTA TTTGCATTGC TGTAGTAGGA
    ATAGAAGATC CTGTTCGACC AGAGGTGCCT GAAGCAATTC AAAAGTGCCA GCGTGCAGGT
    ATCACAGTGC GCATGGTTAC TGGGGACAAT ATAAACACAG CTCGAGCAAT AGCTACCAAG
    TGTGGTATCC TGCAGCCAGG AGAAGACTTC TTGTGCTTGG AGGGAAAAGA ATTCAACACA
    CTGATCCGAA ATGAAAAGGG AGAGGTAGAA CAGGATAAAT TGGACAAGGT TTGGCCGAGA
    CTTCGTGTTT TGGCCCGGTC ATCACCTACA GATAAACATA CTCTGGTAAA AGGCATCATT
    GACAGCACAG TGGCAGAGCG AAGACAGGTG GTGGCTGTGA CTGGAGATGG AACAAACGAT
    GGACCTGCCC TGAAGAAAGC AGATGTGGGA TTTGCAATGG GCATAGCTGG CACAGATGTT
    GCAAAGGAGG CTTCTGATAT TATCTTGACA GACGACAATT TCACCAGCAT TGTAAAGGCT
    GTAATGTGGG GCCGCAATGT ATACGACAGT ATTTCGAAGT TCCTGCAGTT CCAGCTCACC
    GTAAATGTTG TGGCAGTAAT TGTGGCATTT ACAGGAGCCT GCATAACTCA GGATTCACCA
    CTGAAAGCTG TGCAGATGCT GTGGGTCAAC CTTATCATGG ATACATTTGC ATCTTTAGCT
    TTGGCGACTG AGCCTCCTAC TGATTCGCTT CTTCTACGCA AACCATATGG GCGCAACAAA
    CCCCTGATCT CACGTACAAT GATGAAAAAC ATATTGGGAC ATGCTGTTTA TCAGCTTACC
    ATCATATTTA CATTGCTTTT TGCAGGTGAG AAGTTTTTTG ACATTGACAG TGGGAGAAAT
    GTACCCCTGC ATTCTCCACC TTCTGAACAT TATACAATTG TCTTCAACAC CTTTGTGATG
    ATGCAGCTTT TTAATGAGAT CAATGCCCGC AAGATCCACG GTGAACGTAA TGTCTTTGAG
    AACATCTTCC GGAACCCCAT CTTTTGTGCA GTTGTGCTGG GCACTTTTGG AGCTCAGATA
    ATAATTGTGG AGTTTGGAGG AAAGCCATTC AGTTGCTCTG GCCTTACTTT GTCACAGTGG
    TTCTGGTGTA TCTTCATTGG GGTTGGAGAG CTGTTGTGGG GACAGATGAT TTGCACAATA
    CCCACCAGCC GACTCAAGTT CCTAAAAGAG GCTGGCCATG GGCCACTGAA GGAGGACATT
    CAGCAGGAGG AACTTACAGA GGATGTAGAT GAGATTGACC ATGCAGAAAT GGAGCTTAGG
    AGAGGCCAGA TTTTGTGGTT CAGAGGCCTA AACCGAATAC AGACTCAGAT GGACGTAGTT
    TATACATTCC AGACGGGAGG TACCTCTTTA CAAGGGGCTC TGAGGCGCCA GCCTTCCACC
    ATTAGCCAGC ACCTTGATGC TAAACATGCC TCTAGCCCAA CACACGTAGC TCTGTCCTCT
    ACTACCTCTC CCCCAGCCAC TTCTGCTGCT GCTGCTTCTG TGGGCAATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa42726
    Clone ID Related Accession (Same CDS sequence) XM_018091532.2 , XM_018091532.1
    Accession Version XM_018091532.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3471bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product plasma membrane calcium-transporting ATPase 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCAAATA ACTCCACAGA CCATCATTCT GCTAACATTG TAAATGAAGG CAGCTATGAA 
    GGAGATTTTG GCTGCACAAT AATGGACTTA CGGGAGCTTA TGGAATTACG CAGTGCAGAG
    GCAGTCAACC GTATCAGGGA TACTTATGGA GGGGTTCACA ACATCTGCCG CAGGCTAAAG
    ACTTCACCTG TAGAAGGTTT GTCAGGTAAC CCCTCAGACC TCGAGAGGAG GAGACAAATA
    TTTGGTAAAA ACTTCATCCC TCCAAAAAAA GCCAAAACTT TCTTGCAGCT GGTGTGGGAA
    GCACTGCAGG ACGTTACCTT AATTATCCTG GAAATAGCAG CCATAATATC TCTGGGTCTT
    TCTTTCTACC ACCCACCAGG AGGAGATAAT GAATTATGTG GGGAAGCTGC TGCAGGAGTG
    GAGGATGAAG GTGAGGCTCA AGCTGGATGG ATTGAAGGGG CAGCCATCTT ATTCTCGGTC
    ATCATAGTTG TGCTTGTTAC CGCCTTCAAT GACTGGAGCA AAGAGAAGCA ATTTCGAGGC
    CTACAGAGCC GAATTGAGCA GGAGCAGAAA TTCACTGTTA TCCGCAAAGG ACAGGTTATC
    CAAATCCCTG TAGCAGAGCT AGTAGTCGGT GATATTGCAC AAATAAAATA TGGTGACCTC
    CTTCCTGCTG ATGGGATATT GATTCAAGGC AATGACTTAA AAATTGATGA AAGCTCTCTA
    ACTGGGGAGA GCGATCAGGT GAAAAAATCA TTAGAGAAAG ACCCCATGCT TTTGTCAGGA
    ACACACGTAA TGGAAGGGTC TGGACGTATG GTAGTCACAG CAGTAGGTGT CAACTCTCAA
    ACTGGCATAA TATTCACTTT GCTAGGTGCA AATGAGGGAG GTGAAGAGGA AAAAAAATCC
    AAAAAAGCCA AAGCTCAAGA TGGTGTTGCA TTAGAGATCC AACCACTGAA AAGTGAAGAG
    GGCGTAGAGT GTGAAGAGAA AGAGAAAAAG GTTGTGAAAG TGCCAAAGAA AGAAAAGTCT
    GTCCTTCAGG GGAAGCTAAC TCGGCTGGCA GTGCAAATTG GAAAAGCAGG TCTCATTATG
    TCAGCCATCA CAGTGATCAT TTTGGTGCTC TATTTTGTCA TTTATACATT TGGAGTGTTA
    GGTCGCCCAT GGTTGGCAGA ATGCACTCCC ATCTACATCC AGTACTTTGT CAAATTTTTC
    ATTATTGGTG TCACTGTGTT GGTGGTGGCT GTCCCTGAGG GGCTGCCTCT CGCTGTAACA
    ATATCTTTGG CCTACTCTGT AAAGAAAATG ATGAAAGACA ATAATCTTGT ACGTCACTTG
    GATGCCTGTG AAACTATGGG CAATGCAACA GCCATATGCT CTGATAAAAC TGGAACGCTC
    ACTATGAACC GAATGACTGT AGTACAGGCA TTTGTTGGTG GTACTCATTA TAGGCAGATT
    CCTGATCCGG AGGCACTGAA CACAAAAATT CTTGATCTTA TAGTAAATGG GATTTCAGTC
    AACTCAGCAT ACACCTCCAA GATCCTGCCC CCTGAAAAGG AGGGAGGTCT CCCCAGGCAG
    GTGGGTAACA AGACAGAATG TGCGCTACTG GGATTTGTTT TGGACCTGAA GCAAGATTAT
    CAGACAGTGC GAAACGAAAT TCCTGAAGAG AACCTCTACA AAGTTTATAC GTTCAATTCT
    GTGCGAAAAT CTATGAGCAC AGTGTTGTGT GACTCTTCTG GAAAATTCCG CATGTACAGC
    AAAGGGGCCT CTGAAATCAT CTTGCGCAAA TGCACCCGAA TCCTGGACCA AGGAGGAGAT
    GTGTGCCCCT TTAAAGCAAA GGACCGGGAT GAGATGGTAA AGAAAGTAAT TGAACCTATG
    GCATGCGATG GCTTGCGCAC AATCTGCCTA GCATATCGTG ACTTCCCTTC AGATAGTGAG
    CCTAATTGGG ATAATGAGGG CGACATCCTC TCTGACCTTA TTTGCATTGC TGTAGTAGGA
    ATAGAAGATC CTGTTCGACC AGAGGTGCCT GAAGCAATTC AAAAGTGCCA GCGTGCAGGT
    ATCACAGTGC GCATGGTTAC TGGGGACAAT ATAAACACAG CTCGAGCAAT AGCTACCAAG
    TGTGGTATCC TGCAGCCAGG AGAAGACTTC TTGTGCTTGG AGGGAAAAGA ATTCAACACA
    CTGATCCGAA ATGAAAAGGG AGAGGTAGAA CAGGATAAAT TGGACAAGGT TTGGCCGAGA
    CTTCGTGTTT TGGCCCGGTC ATCACCTACA GATAAACATA CTCTGGTAAA AGGCATCATT
    GACAGCACAG TGGCAGAGCG AAGACAGGTG GTGGCTGTGA CTGGAGATGG AACAAACGAT
    GGACCTGCCC TGAAGAAAGC AGATGTGGGA TTTGCAATGG GCATAGCTGG CACAGATGTT
    GCAAAGGAGG CTTCTGATAT TATCTTGACA GACGACAATT TCACCAGCAT TGTAAAGGCT
    GTAATGTGGG GCCGCAATGT ATACGACAGT ATTTCGAAGT TCCTGCAGTT CCAGCTCACC
    GTAAATGTTG TGGCAGTAAT TGTGGCATTT ACAGGAGCCT GCATAACTCA GGATTCACCA
    CTGAAAGCTG TGCAGATGCT GTGGGTCAAC CTTATCATGG ATACATTTGC ATCTTTAGCT
    TTGGCGACTG AGCCTCCTAC TGATTCGCTT CTTCTACGCA AACCATATGG GCGCAACAAA
    CCCCTGATCT CACGTACAAT GATGAAAAAC ATATTGGGAC ATGCTGTTTA TCAGCTTACC
    ATCATATTTA CATTGCTTTT TGCAGGTGAG AAGTTTTTTG ACATTGACAG TGGGAGAAAT
    GTACCCCTGC ATTCTCCACC TTCTGAACAT TATACAATTG TCTTCAACAC CTTTGTGATG
    ATGCAGCTTT TTAATGAGAT CAATGCCCGC AAGATCCACG GTGAACGTAA TGTCTTTGAG
    AACATCTTCC GGAACCCCAT CTTTTGTGCA GTTGTGCTGG GCACTTTTGG AGCTCAGATA
    ATAATTGTGG AGTTTGGAGG AAAGCCATTC AGTTGCTCTG GCCTTACTTT GTCACAGTGG
    TTCTGGTGTA TCTTCATTGG GGTTGGAGAG CTGTTGTGGG GACAGATGAT TTGCACAATA
    CCCACCAGCC GACTCAAGTT CCTAAAAGAG GCTGGCCATG GGCCACTGAA GGAGGACATT
    CAGCAGGAGG AACTTACAGA GGATGTAGAT GAGATTGACC ATGCAGAAAT GGAGCTTAGG
    AGAGGCCAGA TTTTGTGGTT CAGAGGCCTA AACCGAATAC AGACTCAGAT GGACGTAGTT
    TATACATTCC AGACGGGAGG TACCTCTTTA CAAGGGGCTC TGAGGCGCCA GCCTTCCACC
    ATTAGCCAGC ACCTTGATGC TAAACATGCC TCTAGCCCAA CACACGTAGC TCTGTCCTCT
    ACTACCTCTC CCCCAGCCAC TTCTGCTGCT GCTGCTTCTG TGGGCAATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947021.1
    CDS696..4166
    Translation

    Target ORF information:

    RefSeq Version XM_018091532.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis plasma membrane calcium-transporting ATPase 4 (LOC100488346), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091532.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCAAATA ACTCCACAGA CCATCATTCT GCTAACATTG TAAATGAAGG CAGCTATGAA 
    GGAGATTTTG GCTGCACAAT AATGGACTTA CGGGAGCTTA TGGAATTACG CAGTGCAGAG
    GCAGTCAACC GTATCAGGGA TACTTATGGA GGGGTTCACA ACATCTGCCG CAGGCTAAAG
    ACTTCACCTG TAGAAGGTTT GTCAGGTAAC CCCTCAGACC TCGAGAGGAG GAGACAAATA
    TTTGGTAAAA ACTTCATCCC TCCAAAAAAA GCCAAAACTT TCTTGCAGCT GGTGTGGGAA
    GCACTGCAGG ACGTTACCTT AATTATCCTG GAAATAGCAG CCATAATATC TCTGGGTCTT
    TCTTTCTACC ACCCACCAGG AGGAGATAAT GAATTATGTG GGGAAGCTGC TGCAGGAGTG
    GAGGATGAAG GTGAGGCTCA AGCTGGATGG ATTGAAGGGG CAGCCATCTT ATTCTCGGTC
    ATCATAGTTG TGCTTGTTAC CGCCTTCAAT GACTGGAGCA AAGAGAAGCA ATTTCGAGGC
    CTACAGAGCC GAATTGAGCA GGAGCAGAAA TTCACTGTTA TCCGCAAAGG ACAGGTTATC
    CAAATCCCTG TAGCAGAGCT AGTAGTCGGT GATATTGCAC AAATAAAATA TGGTGACCTC
    CTTCCTGCTG ATGGGATATT GATTCAAGGC AATGACTTAA AAATTGATGA AAGCTCTCTA
    ACTGGGGAGA GCGATCAGGT GAAAAAATCA TTAGAGAAAG ACCCCATGCT TTTGTCAGGA
    ACACACGTAA TGGAAGGGTC TGGACGTATG GTAGTCACAG CAGTAGGTGT CAACTCTCAA
    ACTGGCATAA TATTCACTTT GCTAGGTGCA AATGAGGGAG GTGAAGAGGA AAAAAAATCC
    AAAAAAGCCA AAGCTCAAGA TGGTGTTGCA TTAGAGATCC AACCACTGAA AAGTGAAGAG
    GGCGTAGAGT GTGAAGAGAA AGAGAAAAAG GTTGTGAAAG TGCCAAAGAA AGAAAAGTCT
    GTCCTTCAGG GGAAGCTAAC TCGGCTGGCA GTGCAAATTG GAAAAGCAGG TCTCATTATG
    TCAGCCATCA CAGTGATCAT TTTGGTGCTC TATTTTGTCA TTTATACATT TGGAGTGTTA
    GGTCGCCCAT GGTTGGCAGA ATGCACTCCC ATCTACATCC AGTACTTTGT CAAATTTTTC
    ATTATTGGTG TCACTGTGTT GGTGGTGGCT GTCCCTGAGG GGCTGCCTCT CGCTGTAACA
    ATATCTTTGG CCTACTCTGT AAAGAAAATG ATGAAAGACA ATAATCTTGT ACGTCACTTG
    GATGCCTGTG AAACTATGGG CAATGCAACA GCCATATGCT CTGATAAAAC TGGAACGCTC
    ACTATGAACC GAATGACTGT AGTACAGGCA TTTGTTGGTG GTACTCATTA TAGGCAGATT
    CCTGATCCGG AGGCACTGAA CACAAAAATT CTTGATCTTA TAGTAAATGG GATTTCAGTC
    AACTCAGCAT ACACCTCCAA GATCCTGCCC CCTGAAAAGG AGGGAGGTCT CCCCAGGCAG
    GTGGGTAACA AGACAGAATG TGCGCTACTG GGATTTGTTT TGGACCTGAA GCAAGATTAT
    CAGACAGTGC GAAACGAAAT TCCTGAAGAG AACCTCTACA AAGTTTATAC GTTCAATTCT
    GTGCGAAAAT CTATGAGCAC AGTGTTGTGT GACTCTTCTG GAAAATTCCG CATGTACAGC
    AAAGGGGCCT CTGAAATCAT CTTGCGCAAA TGCACCCGAA TCCTGGACCA AGGAGGAGAT
    GTGTGCCCCT TTAAAGCAAA GGACCGGGAT GAGATGGTAA AGAAAGTAAT TGAACCTATG
    GCATGCGATG GCTTGCGCAC AATCTGCCTA GCATATCGTG ACTTCCCTTC AGATAGTGAG
    CCTAATTGGG ATAATGAGGG CGACATCCTC TCTGACCTTA TTTGCATTGC TGTAGTAGGA
    ATAGAAGATC CTGTTCGACC AGAGGTGCCT GAAGCAATTC AAAAGTGCCA GCGTGCAGGT
    ATCACAGTGC GCATGGTTAC TGGGGACAAT ATAAACACAG CTCGAGCAAT AGCTACCAAG
    TGTGGTATCC TGCAGCCAGG AGAAGACTTC TTGTGCTTGG AGGGAAAAGA ATTCAACACA
    CTGATCCGAA ATGAAAAGGG AGAGGTAGAA CAGGATAAAT TGGACAAGGT TTGGCCGAGA
    CTTCGTGTTT TGGCCCGGTC ATCACCTACA GATAAACATA CTCTGGTAAA AGGCATCATT
    GACAGCACAG TGGCAGAGCG AAGACAGGTG GTGGCTGTGA CTGGAGATGG AACAAACGAT
    GGACCTGCCC TGAAGAAAGC AGATGTGGGA TTTGCAATGG GCATAGCTGG CACAGATGTT
    GCAAAGGAGG CTTCTGATAT TATCTTGACA GACGACAATT TCACCAGCAT TGTAAAGGCT
    GTAATGTGGG GCCGCAATGT ATACGACAGT ATTTCGAAGT TCCTGCAGTT CCAGCTCACC
    GTAAATGTTG TGGCAGTAAT TGTGGCATTT ACAGGAGCCT GCATAACTCA GGATTCACCA
    CTGAAAGCTG TGCAGATGCT GTGGGTCAAC CTTATCATGG ATACATTTGC ATCTTTAGCT
    TTGGCGACTG AGCCTCCTAC TGATTCGCTT CTTCTACGCA AACCATATGG GCGCAACAAA
    CCCCTGATCT CACGTACAAT GATGAAAAAC ATATTGGGAC ATGCTGTTTA TCAGCTTACC
    ATCATATTTA CATTGCTTTT TGCAGGTGAG AAGTTTTTTG ACATTGACAG TGGGAGAAAT
    GTACCCCTGC ATTCTCCACC TTCTGAACAT TATACAATTG TCTTCAACAC CTTTGTGATG
    ATGCAGCTTT TTAATGAGAT CAATGCCCGC AAGATCCACG GTGAACGTAA TGTCTTTGAG
    AACATCTTCC GGAACCCCAT CTTTTGTGCA GTTGTGCTGG GCACTTTTGG AGCTCAGATA
    ATAATTGTGG AGTTTGGAGG AAAGCCATTC AGTTGCTCTG GCCTTACTTT GTCACAGTGG
    TTCTGGTGTA TCTTCATTGG GGTTGGAGAG CTGTTGTGGG GACAGATGAT TTGCACAATA
    CCCACCAGCC GACTCAAGTT CCTAAAAGAG GCTGGCCATG GGCCACTGAA GGAGGACATT
    CAGCAGGAGG AACTTACAGA GGATGTAGAT GAGATTGACC ATGCAGAAAT GGAGCTTAGG
    AGAGGCCAGA TTTTGTGGTT CAGAGGCCTA AACCGAATAC AGACTCAGAT GGACGTAGTT
    TATACATTCC AGACGGGAGG TACCTCTTTA CAAGGGGCTC TGAGGCGCCA GCCTTCCACC
    ATTAGCCAGC ACCTTGATGC TAAACATGCC TCTAGCCCAA CACACGTAGC TCTGTCCTCT
    ACTACCTCTC CCCCAGCCAC TTCTGCTGCT GCTGCTTCTG TGGGCAATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52243
    Clone ID Related Accession (Same CDS sequence) XM_031896858.1
    Accession Version XM_031896858.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3735bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product plasma membrane calcium-transporting ATPase 4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCAAATA ACTCCACAGA CCATCATTCT GCTAACATTG TAAATGAAGG CAGCTATGAA 
    GGAGATTTTG GCTGCACAAT AATGGACTTA CGGGAGCTTA TGGAATTACG CAGTGCAGAG
    GCAGTCAACC GTATCAGGGA TACTTATGGA GGGGTTCACA ACATCTGCCG CAGGCTAAAG
    ACTTCACCTG TAGAAGGTTT GTCAGGTAAC CCCTCAGACC TCGAGAGGAG GAGACAAATA
    TTTGGTAAAA ACTTCATCCC TCCAAAAAAA GCCAAAACTT TCTTGCAGCT GGTGTGGGAA
    GCACTGCAGG ACGTTACCTT AATTATCCTG GAAATAGCAG CCATAATATC TCTGGGTCTT
    TCTTTCTACC ACCCACCAGG AGGAGATAAT GAATTATGTG GGGAAGCTGC TGCAGGAGTG
    GAGGATGAAG GTGAGGCTCA AGCTGGATGG ATTGAAGGGG CAGCCATCTT ATTCTCGGTC
    ATCATAGTTG TGCTTGTTAC CGCCTTCAAT GACTGGAGCA AAGAGAAGCA ATTTCGAGGC
    CTACAGAGCC GAATTGAGCA GGAGCAGAAA TTCACTGTTA TCCGCAAAGG ACAGGTTATC
    CAAATCCCTG TAGCAGAGCT AGTAGTCGGT GATATTGCAC AAATAAAATA TGGTGACCTC
    CTTCCTGCTG ATGGGATATT GATTCAAGGC AATGACTTAA AAATTGATGA AAGCTCTCTA
    ACTGGGGAGA GCGATCAGGT GAAAAAATCA TTAGAGAAAG ACCCCATGCT TTTGTCAGGA
    ACACACGTAA TGGAAGGGTC TGGACGTATG GTAGTCACAG CAGTAGGTGT CAACTCTCAA
    ACTGGCATAA TATTCACTTT GCTAGGTGCA AATGAGGGAG GTGAAGAGGA AAAAAAATCC
    AAAAAAGGTA AAAAACCAGG ACCCTCAGAA AATCGCAACA AAGCCAAAGC TCAAGATGGT
    GTTGCATTAG AGATCCAACC ACTGAAAAGT GAAGAGGGCG TAGAGTGTGA AGAGAAAGAG
    AAAAAGGTTG TGAAAGTGCC AAAGAAAGAA AAGTCTGTCC TTCAGGGGAA GCTAACTCGG
    CTGGCAGTGC AAATTGGAAA AGCAGGTCTC ATTATGTCAG CCATCACAGT GATCATTTTG
    GTGCTCTATT TTGTCATTTA TACATTTGGA GTGTTAGGTC GCCCATGGTT GGCAGAATGC
    ACTCCCATCT ACATCCAGTA CTTTGTCAAA TTTTTCATTA TTGGTGTCAC TGTGTTGGTG
    GTGGCTGTCC CTGAGGGGCT GCCTCTCGCT GTAACAATAT CTTTGGCCTA CTCTGTAAAG
    AAAATGATGA AAGACAATAA TCTTGTACGT CACTTGGATG CCTGTGAAAC TATGGGCAAT
    GCAACAGCCA TATGCTCTGA TAAAACTGGA ACGCTCACTA TGAACCGAAT GACTGTAGTA
    CAGGCATTTG TTGGTGGTAC TCATTATAGG CAGATTCCTG ATCCGGAGGC ACTGAACACA
    AAAATTCTTG ATCTTATAGT AAATGGGATT TCAGTCAACT CAGCATACAC CTCCAAGATC
    CTGCCCCCTG AAAAGGAGGG AGGTCTCCCC AGGCAGGTGG GTAACAAGAC AGAATGTGCG
    CTACTGGGAT TTGTTTTGGA CCTGAAGCAA GATTATCAGA CAGTGCGAAA CGAAATTCCT
    GAAGAGAACC TCTACAAAGT TTATACGTTC AATTCTGTGC GAAAATCTAT GAGCACAGTG
    TTGTGTGACT CTTCTGGAAA ATTCCGCATG TACAGCAAAG GGGCCTCTGA AATCATCTTG
    CGCAAATGCA CCCGAATCCT GGACCAAGGA GGAGATGTGT GCCCCTTTAA AGCAAAGGAC
    CGGGATGAGA TGGTAAAGAA AGTAATTGAA CCTATGGCAT GCGATGGCTT GCGCACAATC
    TGCCTAGCAT ATCGTGACTT CCCTTCAGAT AGTGAGCCTA ATTGGGATAA TGAGGGCGAC
    ATCCTCTCTG ACCTTATTTG CATTGCTGTA GTAGGAATAG AAGATCCTGT TCGACCAGAG
    GTGCCTGAAG CAATTCAAAA GTGCCAGCGT GCAGGTATCA CAGTGCGCAT GGTTACTGGG
    GACAATATAA ACACAGCTCG AGCAATAGCT ACCAAGTGTG GTATCCTGCA GCCAGGAGAA
    GACTTCTTGT GCTTGGAGGG AAAAGAATTC AACACACTGA TCCGAAATGA AAAGGGAGAG
    GTAGAACAGG ATAAATTGGA CAAGGTTTGG CCGAGACTTC GTGTTTTGGC CCGGTCATCA
    CCTACAGATA AACATACTCT GGTAAAAGGC ATCATTGACA GCACAGTGGC AGAGCGAAGA
    CAGGTGGTGG CTGTGACTGG AGATGGAACA AACGATGGAC CTGCCCTGAA GAAAGCAGAT
    GTGGGATTTG CAATGGGCAT AGCTGGCACA GATGTTGCAA AGGAGGCTTC TGATATTATC
    TTGACAGACG ACAATTTCAC CAGCATTGTA AAGGCTGTAA TGTGGGGCCG CAATGTATAC
    GACAGTATTT CGAAGTTCCT GCAGTTCCAG CTCACCGTAA ATGTTGTGGC AGTAATTGTG
    GCATTTACAG GAGCCTGCAT AACTCAGGAT TCACCACTGA AAGCTGTGCA GATGCTGTGG
    GTCAACCTTA TCATGGATAC ATTTGCATCT TTAGCTTTGG CGACTGAGCC TCCTACTGAT
    TCGCTTCTTC TACGCAAACC ATATGGGCGC AACAAACCCC TGATCTCACG TACAATGATG
    AAAAACATAT TGGGACATGC TGTTTATCAG CTTACCATCA TATTTACATT GCTTTTTGCA
    GGTGAGAAGT TTTTTGACAT TGACAGTGGG AGAAATGTAC CCCTGCATTC TCCACCTTCT
    GAACATTATA CAATTGTCTT CAACACCTTT GTGATGATGC AGCTTTTTAA TGAGATCAAT
    GCCCGCAAGA TCCACGGTGA ACGTAATGTC TTTGAGAACA TCTTCCGGAA CCCCATCTTT
    TGTGCAGTTG TGCTGGGCAC TTTTGGAGCT CAGATAATAA TTGTGGAGTT TGGAGGAAAG
    CCATTCAGTT GCTCTGGCCT TACTTTGTCA CAGTGGTTCT GGTGTATCTT CATTGGGGTT
    GGAGAGCTGT TGTGGGGACA GATGATTTGC ACAATACCCA CCAGCCGACT CAAGTTCCTA
    AAAGAGGCTG GCCATGGGCC ACTGAAGGAG GACATTCAGC AGGAGGAACT TACAGAGGAT
    GTAGATGAGA TTGACCATGC AGAAATGGAG CTTAGGAGAG GCCAGATTTT GTGGTTCAGA
    GGCCTAAACC GAATACAGAC TCAGATGGAC GTAGTTTATA CATTCCAGAC GGGAGGTACC
    TCTTTACAAG GGGCTCTGAG GCGCCAGCCT TCCACCATTA GCCAGCACCT TGATGCTAAA
    CATGCCTCTA GCCCAACACA CATTAAAGTT GTGAATGCGT TCCGAAGCTC TCTCTACGAG
    GGGCTAGAGA AGCCACAGAT CCGCAGTGCC ATCCATAGCT TCATGACCCA CCCAGAGTTC
    ACTCCACGGG AGGCAGGACC TGCCCCCCAA TTCGAAGACA GCGACAGCGA GGGTGAGGAA
    TCTAAGGGTC TCTCAAAGGC GCATAAAGAT CAAACCCATA ATCACAACAA CAATTCTTCT
    GTTGACCCAC ATGCATTGGA TATCACAGTG TCTCTATCTG ATAGCCCATT GCATAGCATG
    GAAACCTCCA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031752718.1
    CDS666..4400
    Translation

    Target ORF information:

    RefSeq Version XM_031896858.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis plasma membrane calcium-transporting ATPase 4 (LOC100488346), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896858.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCAAATA ACTCCACAGA CCATCATTCT GCTAACATTG TAAATGAAGG CAGCTATGAA 
    GGAGATTTTG GCTGCACAAT AATGGACTTA CGGGAGCTTA TGGAATTACG CAGTGCAGAG
    GCAGTCAACC GTATCAGGGA TACTTATGGA GGGGTTCACA ACATCTGCCG CAGGCTAAAG
    ACTTCACCTG TAGAAGGTTT GTCAGGTAAC CCCTCAGACC TCGAGAGGAG GAGACAAATA
    TTTGGTAAAA ACTTCATCCC TCCAAAAAAA GCCAAAACTT TCTTGCAGCT GGTGTGGGAA
    GCACTGCAGG ACGTTACCTT AATTATCCTG GAAATAGCAG CCATAATATC TCTGGGTCTT
    TCTTTCTACC ACCCACCAGG AGGAGATAAT GAATTATGTG GGGAAGCTGC TGCAGGAGTG
    GAGGATGAAG GTGAGGCTCA AGCTGGATGG ATTGAAGGGG CAGCCATCTT ATTCTCGGTC
    ATCATAGTTG TGCTTGTTAC CGCCTTCAAT GACTGGAGCA AAGAGAAGCA ATTTCGAGGC
    CTACAGAGCC GAATTGAGCA GGAGCAGAAA TTCACTGTTA TCCGCAAAGG ACAGGTTATC
    CAAATCCCTG TAGCAGAGCT AGTAGTCGGT GATATTGCAC AAATAAAATA TGGTGACCTC
    CTTCCTGCTG ATGGGATATT GATTCAAGGC AATGACTTAA AAATTGATGA AAGCTCTCTA
    ACTGGGGAGA GCGATCAGGT GAAAAAATCA TTAGAGAAAG ACCCCATGCT TTTGTCAGGA
    ACACACGTAA TGGAAGGGTC TGGACGTATG GTAGTCACAG CAGTAGGTGT CAACTCTCAA
    ACTGGCATAA TATTCACTTT GCTAGGTGCA AATGAGGGAG GTGAAGAGGA AAAAAAATCC
    AAAAAAGGTA AAAAACCAGG ACCCTCAGAA AATCGCAACA AAGCCAAAGC TCAAGATGGT
    GTTGCATTAG AGATCCAACC ACTGAAAAGT GAAGAGGGCG TAGAGTGTGA AGAGAAAGAG
    AAAAAGGTTG TGAAAGTGCC AAAGAAAGAA AAGTCTGTCC TTCAGGGGAA GCTAACTCGG
    CTGGCAGTGC AAATTGGAAA AGCAGGTCTC ATTATGTCAG CCATCACAGT GATCATTTTG
    GTGCTCTATT TTGTCATTTA TACATTTGGA GTGTTAGGTC GCCCATGGTT GGCAGAATGC
    ACTCCCATCT ACATCCAGTA CTTTGTCAAA TTTTTCATTA TTGGTGTCAC TGTGTTGGTG
    GTGGCTGTCC CTGAGGGGCT GCCTCTCGCT GTAACAATAT CTTTGGCCTA CTCTGTAAAG
    AAAATGATGA AAGACAATAA TCTTGTACGT CACTTGGATG CCTGTGAAAC TATGGGCAAT
    GCAACAGCCA TATGCTCTGA TAAAACTGGA ACGCTCACTA TGAACCGAAT GACTGTAGTA
    CAGGCATTTG TTGGTGGTAC TCATTATAGG CAGATTCCTG ATCCGGAGGC ACTGAACACA
    AAAATTCTTG ATCTTATAGT AAATGGGATT TCAGTCAACT CAGCATACAC CTCCAAGATC
    CTGCCCCCTG AAAAGGAGGG AGGTCTCCCC AGGCAGGTGG GTAACAAGAC AGAATGTGCG
    CTACTGGGAT TTGTTTTGGA CCTGAAGCAA GATTATCAGA CAGTGCGAAA CGAAATTCCT
    GAAGAGAACC TCTACAAAGT TTATACGTTC AATTCTGTGC GAAAATCTAT GAGCACAGTG
    TTGTGTGACT CTTCTGGAAA ATTCCGCATG TACAGCAAAG GGGCCTCTGA AATCATCTTG
    CGCAAATGCA CCCGAATCCT GGACCAAGGA GGAGATGTGT GCCCCTTTAA AGCAAAGGAC
    CGGGATGAGA TGGTAAAGAA AGTAATTGAA CCTATGGCAT GCGATGGCTT GCGCACAATC
    TGCCTAGCAT ATCGTGACTT CCCTTCAGAT AGTGAGCCTA ATTGGGATAA TGAGGGCGAC
    ATCCTCTCTG ACCTTATTTG CATTGCTGTA GTAGGAATAG AAGATCCTGT TCGACCAGAG
    GTGCCTGAAG CAATTCAAAA GTGCCAGCGT GCAGGTATCA CAGTGCGCAT GGTTACTGGG
    GACAATATAA ACACAGCTCG AGCAATAGCT ACCAAGTGTG GTATCCTGCA GCCAGGAGAA
    GACTTCTTGT GCTTGGAGGG AAAAGAATTC AACACACTGA TCCGAAATGA AAAGGGAGAG
    GTAGAACAGG ATAAATTGGA CAAGGTTTGG CCGAGACTTC GTGTTTTGGC CCGGTCATCA
    CCTACAGATA AACATACTCT GGTAAAAGGC ATCATTGACA GCACAGTGGC AGAGCGAAGA
    CAGGTGGTGG CTGTGACTGG AGATGGAACA AACGATGGAC CTGCCCTGAA GAAAGCAGAT
    GTGGGATTTG CAATGGGCAT AGCTGGCACA GATGTTGCAA AGGAGGCTTC TGATATTATC
    TTGACAGACG ACAATTTCAC CAGCATTGTA AAGGCTGTAA TGTGGGGCCG CAATGTATAC
    GACAGTATTT CGAAGTTCCT GCAGTTCCAG CTCACCGTAA ATGTTGTGGC AGTAATTGTG
    GCATTTACAG GAGCCTGCAT AACTCAGGAT TCACCACTGA AAGCTGTGCA GATGCTGTGG
    GTCAACCTTA TCATGGATAC ATTTGCATCT TTAGCTTTGG CGACTGAGCC TCCTACTGAT
    TCGCTTCTTC TACGCAAACC ATATGGGCGC AACAAACCCC TGATCTCACG TACAATGATG
    AAAAACATAT TGGGACATGC TGTTTATCAG CTTACCATCA TATTTACATT GCTTTTTGCA
    GGTGAGAAGT TTTTTGACAT TGACAGTGGG AGAAATGTAC CCCTGCATTC TCCACCTTCT
    GAACATTATA CAATTGTCTT CAACACCTTT GTGATGATGC AGCTTTTTAA TGAGATCAAT
    GCCCGCAAGA TCCACGGTGA ACGTAATGTC TTTGAGAACA TCTTCCGGAA CCCCATCTTT
    TGTGCAGTTG TGCTGGGCAC TTTTGGAGCT CAGATAATAA TTGTGGAGTT TGGAGGAAAG
    CCATTCAGTT GCTCTGGCCT TACTTTGTCA CAGTGGTTCT GGTGTATCTT CATTGGGGTT
    GGAGAGCTGT TGTGGGGACA GATGATTTGC ACAATACCCA CCAGCCGACT CAAGTTCCTA
    AAAGAGGCTG GCCATGGGCC ACTGAAGGAG GACATTCAGC AGGAGGAACT TACAGAGGAT
    GTAGATGAGA TTGACCATGC AGAAATGGAG CTTAGGAGAG GCCAGATTTT GTGGTTCAGA
    GGCCTAAACC GAATACAGAC TCAGATGGAC GTAGTTTATA CATTCCAGAC GGGAGGTACC
    TCTTTACAAG GGGCTCTGAG GCGCCAGCCT TCCACCATTA GCCAGCACCT TGATGCTAAA
    CATGCCTCTA GCCCAACACA CATTAAAGTT GTGAATGCGT TCCGAAGCTC TCTCTACGAG
    GGGCTAGAGA AGCCACAGAT CCGCAGTGCC ATCCATAGCT TCATGACCCA CCCAGAGTTC
    ACTCCACGGG AGGCAGGACC TGCCCCCCAA TTCGAAGACA GCGACAGCGA GGGTGAGGAA
    TCTAAGGGTC TCTCAAAGGC GCATAAAGAT CAAACCCATA ATCACAACAA CAATTCTTCT
    GTTGACCCAC ATGCATTGGA TATCACAGTG TCTCTATCTG ATAGCCCATT GCATAGCATG
    GAAACCTCCA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa03801
    Clone ID Related Accession (Same CDS sequence) XM_004910677.3 , XM_012970635.2 , XM_012970637.2 , XM_012970637.3 , XM_004910677.4 , XM_012970635.3
    Accession Version XM_004910677.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3618bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product plasma membrane calcium-transporting ATPase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004910677.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATA ACTCCACAGA CCATCATTCT GCTAACATTG TAAATGAAGG CAGCTATGAA 
    GGAGATTTTG GCTGCACAAT AATGGACTTA CGGGAGCTTA TGGAATTACG CAGTGCAGAG
    GCAGTCAACC GTATCAGGGA TACTTATGGA GGGGTTCACA ACATCTGCCG CAGGCTAAAG
    ACTTCACCTG TAGAAGGTTT GTCAGGTAAC CCCTCAGACC TCGAGAGGAG GAGACAAATA
    TTTGGTAAAA ACTTCATCCC TCCAAAAAAA GCCAAAACTT TCTTGCAGCT GGTGTGGGAA
    GCACTGCAGG ACGTTACCTT AATTATCCTG GAAATAGCAG CCATAATATC TCTGGGTCTT
    TCTTTCTACC ACCCACCAGG AGGAGATAAT GAATTATGTG GGGAAGCTGC TGCAGGAGTG
    GAGGATGAAG GTGAGGCTCA AGCTGGATGG ATTGAAGGGG CAGCCATCTT ATTCTCGGTC
    ATCATAGTTG TGCTTGTTAC CGCCTTCAAT GACTGGAGCA AAGAGAAGCA ATTTCGAGGC
    CTACAGAGCC GAATTGAGCA GGAGCAGAAA TTCACTGTTA TCCGCAAAGG ACAGGTTATC
    CAAATCCCTG TAGCAGAGCT AGTAGTCGGT GATATTGCAC AAATAAAATA TGGTGACCTC
    CTTCCTGCTG ATGGGATATT GATTCAAGGC AATGACTTAA AAATTGATGA AAGCTCTCTA
    ACTGGGGAGA GCGATCAGGT GAAAAAATCA TTAGAGAAAG ACCCCATGCT TTTGTCAGGA
    ACACACGTAA TGGAAGGGTC TGGACGTATG GTAGTCACAG CAGTAGGTGT CAACTCTCAA
    ACTGGCATAA TATTCACTTT GCTAGGTGCA AATGAGGGAG GTGAAGAGGA AAAAAAATCC
    AAAAAAGGTA AAAAACCAGG ACCCTCAGAA AATCGCAACA AAGCCAAAGC TCAAGATGGT
    GTTGCATTAG AGATCCAACC ACTGAAAAGT GAAGAGGGCG TAGAGTGTGA AGAGAAAGAG
    AAAAAGGTTG TGAAAGTGCC AAAGAAAGAA AAGTCTGTCC TTCAGGGGAA GCTAACTCGG
    CTGGCAGTGC AAATTGGAAA AGCAGGTCTC ATTATGTCAG CCATCACAGT GATCATTTTG
    GTGCTCTATT TTGTCATTTA TACATTTGGA GTGTTAGGTC GCCCATGGTT GGCAGAATGC
    ACTCCCATCT ACATCCAGTA CTTTGTCAAA TTTTTCATTA TTGGTGTCAC TGTGTTGGTG
    GTGGCTGTCC CTGAGGGGCT GCCTCTCGCT GTAACAATAT CTTTGGCCTA CTCTGTAAAG
    AAAATGATGA AAGACAATAA TCTTGTACGT CACTTGGATG CCTGTGAAAC TATGGGCAAT
    GCAACAGCCA TATGCTCTGA TAAAACTGGA ACGCTCACTA TGAACCGAAT GACTGTAGTA
    CAGGCATTTG TTGGTGGTAC TCATTATAGG CAGATTCCTG ATCCGGAGGC ACTGAACACA
    AAAATTCTTG ATCTTATAGT AAATGGGATT TCAGTCAACT CAGCATACAC CTCCAAGATC
    CTGCCCCCTG AAAAGGAGGG AGGTCTCCCC AGGCAGGTGG GTAACAAGAC AGAATGTGCG
    CTACTGGGAT TTGTTTTGGA CCTGAAGCAA GATTATCAGA CAGTGCGAAA CGAAATTCCT
    GAAGAGAACC TCTACAAAGT TTATACGTTC AATTCTGTGC GAAAATCTAT GAGCACAGTG
    TTGTGTGACT CTTCTGGAAA ATTCCGCATG TACAGCAAAG GGGCCTCTGA AATCATCTTG
    CGCAAATGCA CCCGAATCCT GGACCAAGGA GGAGATGTGT GCCCCTTTAA AGCAAAGGAC
    CGGGATGAGA TGGTAAAGAA AGTAATTGAA CCTATGGCAT GCGATGGCTT GCGCACAATC
    TGCCTAGCAT ATCGTGACTT CCCTTCAGAT AGTGAGCCTA ATTGGGATAA TGAGGGCGAC
    ATCCTCTCTG ACCTTATTTG CATTGCTGTA GTAGGAATAG AAGATCCTGT TCGACCAGAG
    GTGCCTGAAG CAATTCAAAA GTGCCAGCGT GCAGGTATCA CAGTGCGCAT GGTTACTGGG
    GACAATATAA ACACAGCTCG AGCAATAGCT ACCAAGTGTG GTATCCTGCA GCCAGGAGAA
    GACTTCTTGT GCTTGGAGGG AAAAGAATTC AACACACTGA TCCGAAATGA AAAGGGAGAG
    GTAGAACAGG ATAAATTGGA CAAGGTTTGG CCGAGACTTC GTGTTTTGGC CCGGTCATCA
    CCTACAGATA AACATACTCT GGTAAAAGGC ATCATTGACA GCACAGTGGC AGAGCGAAGA
    CAGGTGGTGG CTGTGACTGG AGATGGAACA AACGATGGAC CTGCCCTGAA GAAAGCAGAT
    GTGGGATTTG CAATGGGCAT AGCTGGCACA GATGTTGCAA AGGAGGCTTC TGATATTATC
    TTGACAGACG ACAATTTCAC CAGCATTGTA AAGGCTGTAA TGTGGGGCCG CAATGTATAC
    GACAGTATTT CGAAGTTCCT GCAGTTCCAG CTCACCGTAA ATGTTGTGGC AGTAATTGTG
    GCATTTACAG GAGCCTGCAT AACTCAGGAT TCACCACTGA AAGCTGTGCA GATGCTGTGG
    GTCAACCTTA TCATGGATAC ATTTGCATCT TTAGCTTTGG CGACTGAGCC TCCTACTGAT
    TCGCTTCTTC TACGCAAACC ATATGGGCGC AACAAACCCC TGATCTCACG TACAATGATG
    AAAAACATAT TGGGACATGC TGTTTATCAG CTTACCATCA TATTTACATT GCTTTTTGCA
    GGTGAGAAGT TTTTTGACAT TGACAGTGGG AGAAATGTAC CCCTGCATTC TCCACCTTCT
    GAACATTATA CAATTGTCTT CAACACCTTT GTGATGATGC AGCTTTTTAA TGAGATCAAT
    GCCCGCAAGA TCCACGGTGA ACGTAATGTC TTTGAGAACA TCTTCCGGAA CCCCATCTTT
    TGTGCAGTTG TGCTGGGCAC TTTTGGAGCT CAGATAATAA TTGTGGAGTT TGGAGGAAAG
    CCATTCAGTT GCTCTGGCCT TACTTTGTCA CAGTGGTTCT GGTGTATCTT CATTGGGGTT
    GGAGAGCTGT TGTGGGGACA GATGATTTGC ACAATACCCA CCAGCCGACT CAAGTTCCTA
    AAAGAGGCTG GCCATGGGCC ACTGAAGGAG GACATTCAGC AGGAGGAACT TACAGAGGAT
    GTAGATGAGA TTGACCATGC AGAAATGGAG CTTAGGAGAG GCCAGATTTT GTGGTTCAGA
    GGCCTAAACC GAATACAGAC TCAGATTAAA GTTGTGAATG CGTTCCGAAG CTCTCTCTAC
    GAGGGGCTAG AGAAGCCACA GATCCGCAGT GCCATCCATA GCTTCATGAC CCACCCAGAG
    TTCACTCCAC GGGAGGCAGG ACCTGCCCCC CAATTCGAAG ACAGCGACAG CGAGGGTGAG
    GAATCTAAGG GTCTCTCAAA GGCGCATAAA GATCAAACCC ATAATCACAA CAACAATTCT
    TCTGTTGACC CACATGCATT GGATATCACA GTGTCTCTAT CTGATAGCCC ATTGCATAGC
    ATGGAAACCT CCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004910734.1
    CDS693..4310
    Translation

    Target ORF information:

    RefSeq Version XM_004910677.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis plasma membrane calcium-transporting ATPase 4 (LOC100488346), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004910677.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATA ACTCCACAGA CCATCATTCT GCTAACATTG TAAATGAAGG CAGCTATGAA 
    GGAGATTTTG GCTGCACAAT AATGGACTTA CGGGAGCTTA TGGAATTACG CAGTGCAGAG
    GCAGTCAACC GTATCAGGGA TACTTATGGA GGGGTTCACA ACATCTGCCG CAGGCTAAAG
    ACTTCACCTG TAGAAGGTTT GTCAGGTAAC CCCTCAGACC TCGAGAGGAG GAGACAAATA
    TTTGGTAAAA ACTTCATCCC TCCAAAAAAA GCCAAAACTT TCTTGCAGCT GGTGTGGGAA
    GCACTGCAGG ACGTTACCTT AATTATCCTG GAAATAGCAG CCATAATATC TCTGGGTCTT
    TCTTTCTACC ACCCACCAGG AGGAGATAAT GAATTATGTG GGGAAGCTGC TGCAGGAGTG
    GAGGATGAAG GTGAGGCTCA AGCTGGATGG ATTGAAGGGG CAGCCATCTT ATTCTCGGTC
    ATCATAGTTG TGCTTGTTAC CGCCTTCAAT GACTGGAGCA AAGAGAAGCA ATTTCGAGGC
    CTACAGAGCC GAATTGAGCA GGAGCAGAAA TTCACTGTTA TCCGCAAAGG ACAGGTTATC
    CAAATCCCTG TAGCAGAGCT AGTAGTCGGT GATATTGCAC AAATAAAATA TGGTGACCTC
    CTTCCTGCTG ATGGGATATT GATTCAAGGC AATGACTTAA AAATTGATGA AAGCTCTCTA
    ACTGGGGAGA GCGATCAGGT GAAAAAATCA TTAGAGAAAG ACCCCATGCT TTTGTCAGGA
    ACACACGTAA TGGAAGGGTC TGGACGTATG GTAGTCACAG CAGTAGGTGT CAACTCTCAA
    ACTGGCATAA TATTCACTTT GCTAGGTGCA AATGAGGGAG GTGAAGAGGA AAAAAAATCC
    AAAAAAGGTA AAAAACCAGG ACCCTCAGAA AATCGCAACA AAGCCAAAGC TCAAGATGGT
    GTTGCATTAG AGATCCAACC ACTGAAAAGT GAAGAGGGCG TAGAGTGTGA AGAGAAAGAG
    AAAAAGGTTG TGAAAGTGCC AAAGAAAGAA AAGTCTGTCC TTCAGGGGAA GCTAACTCGG
    CTGGCAGTGC AAATTGGAAA AGCAGGTCTC ATTATGTCAG CCATCACAGT GATCATTTTG
    GTGCTCTATT TTGTCATTTA TACATTTGGA GTGTTAGGTC GCCCATGGTT GGCAGAATGC
    ACTCCCATCT ACATCCAGTA CTTTGTCAAA TTTTTCATTA TTGGTGTCAC TGTGTTGGTG
    GTGGCTGTCC CTGAGGGGCT GCCTCTCGCT GTAACAATAT CTTTGGCCTA CTCTGTAAAG
    AAAATGATGA AAGACAATAA TCTTGTACGT CACTTGGATG CCTGTGAAAC TATGGGCAAT
    GCAACAGCCA TATGCTCTGA TAAAACTGGA ACGCTCACTA TGAACCGAAT GACTGTAGTA
    CAGGCATTTG TTGGTGGTAC TCATTATAGG CAGATTCCTG ATCCGGAGGC ACTGAACACA
    AAAATTCTTG ATCTTATAGT AAATGGGATT TCAGTCAACT CAGCATACAC CTCCAAGATC
    CTGCCCCCTG AAAAGGAGGG AGGTCTCCCC AGGCAGGTGG GTAACAAGAC AGAATGTGCG
    CTACTGGGAT TTGTTTTGGA CCTGAAGCAA GATTATCAGA CAGTGCGAAA CGAAATTCCT
    GAAGAGAACC TCTACAAAGT TTATACGTTC AATTCTGTGC GAAAATCTAT GAGCACAGTG
    TTGTGTGACT CTTCTGGAAA ATTCCGCATG TACAGCAAAG GGGCCTCTGA AATCATCTTG
    CGCAAATGCA CCCGAATCCT GGACCAAGGA GGAGATGTGT GCCCCTTTAA AGCAAAGGAC
    CGGGATGAGA TGGTAAAGAA AGTAATTGAA CCTATGGCAT GCGATGGCTT GCGCACAATC
    TGCCTAGCAT ATCGTGACTT CCCTTCAGAT AGTGAGCCTA ATTGGGATAA TGAGGGCGAC
    ATCCTCTCTG ACCTTATTTG CATTGCTGTA GTAGGAATAG AAGATCCTGT TCGACCAGAG
    GTGCCTGAAG CAATTCAAAA GTGCCAGCGT GCAGGTATCA CAGTGCGCAT GGTTACTGGG
    GACAATATAA ACACAGCTCG AGCAATAGCT ACCAAGTGTG GTATCCTGCA GCCAGGAGAA
    GACTTCTTGT GCTTGGAGGG AAAAGAATTC AACACACTGA TCCGAAATGA AAAGGGAGAG
    GTAGAACAGG ATAAATTGGA CAAGGTTTGG CCGAGACTTC GTGTTTTGGC CCGGTCATCA
    CCTACAGATA AACATACTCT GGTAAAAGGC ATCATTGACA GCACAGTGGC AGAGCGAAGA
    CAGGTGGTGG CTGTGACTGG AGATGGAACA AACGATGGAC CTGCCCTGAA GAAAGCAGAT
    GTGGGATTTG CAATGGGCAT AGCTGGCACA GATGTTGCAA AGGAGGCTTC TGATATTATC
    TTGACAGACG ACAATTTCAC CAGCATTGTA AAGGCTGTAA TGTGGGGCCG CAATGTATAC
    GACAGTATTT CGAAGTTCCT GCAGTTCCAG CTCACCGTAA ATGTTGTGGC AGTAATTGTG
    GCATTTACAG GAGCCTGCAT AACTCAGGAT TCACCACTGA AAGCTGTGCA GATGCTGTGG
    GTCAACCTTA TCATGGATAC ATTTGCATCT TTAGCTTTGG CGACTGAGCC TCCTACTGAT
    TCGCTTCTTC TACGCAAACC ATATGGGCGC AACAAACCCC TGATCTCACG TACAATGATG
    AAAAACATAT TGGGACATGC TGTTTATCAG CTTACCATCA TATTTACATT GCTTTTTGCA
    GGTGAGAAGT TTTTTGACAT TGACAGTGGG AGAAATGTAC CCCTGCATTC TCCACCTTCT
    GAACATTATA CAATTGTCTT CAACACCTTT GTGATGATGC AGCTTTTTAA TGAGATCAAT
    GCCCGCAAGA TCCACGGTGA ACGTAATGTC TTTGAGAACA TCTTCCGGAA CCCCATCTTT
    TGTGCAGTTG TGCTGGGCAC TTTTGGAGCT CAGATAATAA TTGTGGAGTT TGGAGGAAAG
    CCATTCAGTT GCTCTGGCCT TACTTTGTCA CAGTGGTTCT GGTGTATCTT CATTGGGGTT
    GGAGAGCTGT TGTGGGGACA GATGATTTGC ACAATACCCA CCAGCCGACT CAAGTTCCTA
    AAAGAGGCTG GCCATGGGCC ACTGAAGGAG GACATTCAGC AGGAGGAACT TACAGAGGAT
    GTAGATGAGA TTGACCATGC AGAAATGGAG CTTAGGAGAG GCCAGATTTT GTGGTTCAGA
    GGCCTAAACC GAATACAGAC TCAGATTAAA GTTGTGAATG CGTTCCGAAG CTCTCTCTAC
    GAGGGGCTAG AGAAGCCACA GATCCGCAGT GCCATCCATA GCTTCATGAC CCACCCAGAG
    TTCACTCCAC GGGAGGCAGG ACCTGCCCCC CAATTCGAAG ACAGCGACAG CGAGGGTGAG
    GAATCTAAGG GTCTCTCAAA GGCGCATAAA GATCAAACCC ATAATCACAA CAACAATTCT
    TCTGTTGACC CACATGCATT GGATATCACA GTGTCTCTAT CTGATAGCCC ATTGCATAGC
    ATGGAAACCT CCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa03801
    Clone ID Related Accession (Same CDS sequence) XM_004910677.3 , XM_012970635.2 , XM_012970637.2 , XM_012970637.3 , XM_004910677.4 , XM_012970635.3
    Accession Version XM_012970635.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3618bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product plasma membrane calcium-transporting ATPase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970635.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATA ACTCCACAGA CCATCATTCT GCTAACATTG TAAATGAAGG CAGCTATGAA 
    GGAGATTTTG GCTGCACAAT AATGGACTTA CGGGAGCTTA TGGAATTACG CAGTGCAGAG
    GCAGTCAACC GTATCAGGGA TACTTATGGA GGGGTTCACA ACATCTGCCG CAGGCTAAAG
    ACTTCACCTG TAGAAGGTTT GTCAGGTAAC CCCTCAGACC TCGAGAGGAG GAGACAAATA
    TTTGGTAAAA ACTTCATCCC TCCAAAAAAA GCCAAAACTT TCTTGCAGCT GGTGTGGGAA
    GCACTGCAGG ACGTTACCTT AATTATCCTG GAAATAGCAG CCATAATATC TCTGGGTCTT
    TCTTTCTACC ACCCACCAGG AGGAGATAAT GAATTATGTG GGGAAGCTGC TGCAGGAGTG
    GAGGATGAAG GTGAGGCTCA AGCTGGATGG ATTGAAGGGG CAGCCATCTT ATTCTCGGTC
    ATCATAGTTG TGCTTGTTAC CGCCTTCAAT GACTGGAGCA AAGAGAAGCA ATTTCGAGGC
    CTACAGAGCC GAATTGAGCA GGAGCAGAAA TTCACTGTTA TCCGCAAAGG ACAGGTTATC
    CAAATCCCTG TAGCAGAGCT AGTAGTCGGT GATATTGCAC AAATAAAATA TGGTGACCTC
    CTTCCTGCTG ATGGGATATT GATTCAAGGC AATGACTTAA AAATTGATGA AAGCTCTCTA
    ACTGGGGAGA GCGATCAGGT GAAAAAATCA TTAGAGAAAG ACCCCATGCT TTTGTCAGGA
    ACACACGTAA TGGAAGGGTC TGGACGTATG GTAGTCACAG CAGTAGGTGT CAACTCTCAA
    ACTGGCATAA TATTCACTTT GCTAGGTGCA AATGAGGGAG GTGAAGAGGA AAAAAAATCC
    AAAAAAGGTA AAAAACCAGG ACCCTCAGAA AATCGCAACA AAGCCAAAGC TCAAGATGGT
    GTTGCATTAG AGATCCAACC ACTGAAAAGT GAAGAGGGCG TAGAGTGTGA AGAGAAAGAG
    AAAAAGGTTG TGAAAGTGCC AAAGAAAGAA AAGTCTGTCC TTCAGGGGAA GCTAACTCGG
    CTGGCAGTGC AAATTGGAAA AGCAGGTCTC ATTATGTCAG CCATCACAGT GATCATTTTG
    GTGCTCTATT TTGTCATTTA TACATTTGGA GTGTTAGGTC GCCCATGGTT GGCAGAATGC
    ACTCCCATCT ACATCCAGTA CTTTGTCAAA TTTTTCATTA TTGGTGTCAC TGTGTTGGTG
    GTGGCTGTCC CTGAGGGGCT GCCTCTCGCT GTAACAATAT CTTTGGCCTA CTCTGTAAAG
    AAAATGATGA AAGACAATAA TCTTGTACGT CACTTGGATG CCTGTGAAAC TATGGGCAAT
    GCAACAGCCA TATGCTCTGA TAAAACTGGA ACGCTCACTA TGAACCGAAT GACTGTAGTA
    CAGGCATTTG TTGGTGGTAC TCATTATAGG CAGATTCCTG ATCCGGAGGC ACTGAACACA
    AAAATTCTTG ATCTTATAGT AAATGGGATT TCAGTCAACT CAGCATACAC CTCCAAGATC
    CTGCCCCCTG AAAAGGAGGG AGGTCTCCCC AGGCAGGTGG GTAACAAGAC AGAATGTGCG
    CTACTGGGAT TTGTTTTGGA CCTGAAGCAA GATTATCAGA CAGTGCGAAA CGAAATTCCT
    GAAGAGAACC TCTACAAAGT TTATACGTTC AATTCTGTGC GAAAATCTAT GAGCACAGTG
    TTGTGTGACT CTTCTGGAAA ATTCCGCATG TACAGCAAAG GGGCCTCTGA AATCATCTTG
    CGCAAATGCA CCCGAATCCT GGACCAAGGA GGAGATGTGT GCCCCTTTAA AGCAAAGGAC
    CGGGATGAGA TGGTAAAGAA AGTAATTGAA CCTATGGCAT GCGATGGCTT GCGCACAATC
    TGCCTAGCAT ATCGTGACTT CCCTTCAGAT AGTGAGCCTA ATTGGGATAA TGAGGGCGAC
    ATCCTCTCTG ACCTTATTTG CATTGCTGTA GTAGGAATAG AAGATCCTGT TCGACCAGAG
    GTGCCTGAAG CAATTCAAAA GTGCCAGCGT GCAGGTATCA CAGTGCGCAT GGTTACTGGG
    GACAATATAA ACACAGCTCG AGCAATAGCT ACCAAGTGTG GTATCCTGCA GCCAGGAGAA
    GACTTCTTGT GCTTGGAGGG AAAAGAATTC AACACACTGA TCCGAAATGA AAAGGGAGAG
    GTAGAACAGG ATAAATTGGA CAAGGTTTGG CCGAGACTTC GTGTTTTGGC CCGGTCATCA
    CCTACAGATA AACATACTCT GGTAAAAGGC ATCATTGACA GCACAGTGGC AGAGCGAAGA
    CAGGTGGTGG CTGTGACTGG AGATGGAACA AACGATGGAC CTGCCCTGAA GAAAGCAGAT
    GTGGGATTTG CAATGGGCAT AGCTGGCACA GATGTTGCAA AGGAGGCTTC TGATATTATC
    TTGACAGACG ACAATTTCAC CAGCATTGTA AAGGCTGTAA TGTGGGGCCG CAATGTATAC
    GACAGTATTT CGAAGTTCCT GCAGTTCCAG CTCACCGTAA ATGTTGTGGC AGTAATTGTG
    GCATTTACAG GAGCCTGCAT AACTCAGGAT TCACCACTGA AAGCTGTGCA GATGCTGTGG
    GTCAACCTTA TCATGGATAC ATTTGCATCT TTAGCTTTGG CGACTGAGCC TCCTACTGAT
    TCGCTTCTTC TACGCAAACC ATATGGGCGC AACAAACCCC TGATCTCACG TACAATGATG
    AAAAACATAT TGGGACATGC TGTTTATCAG CTTACCATCA TATTTACATT GCTTTTTGCA
    GGTGAGAAGT TTTTTGACAT TGACAGTGGG AGAAATGTAC CCCTGCATTC TCCACCTTCT
    GAACATTATA CAATTGTCTT CAACACCTTT GTGATGATGC AGCTTTTTAA TGAGATCAAT
    GCCCGCAAGA TCCACGGTGA ACGTAATGTC TTTGAGAACA TCTTCCGGAA CCCCATCTTT
    TGTGCAGTTG TGCTGGGCAC TTTTGGAGCT CAGATAATAA TTGTGGAGTT TGGAGGAAAG
    CCATTCAGTT GCTCTGGCCT TACTTTGTCA CAGTGGTTCT GGTGTATCTT CATTGGGGTT
    GGAGAGCTGT TGTGGGGACA GATGATTTGC ACAATACCCA CCAGCCGACT CAAGTTCCTA
    AAAGAGGCTG GCCATGGGCC ACTGAAGGAG GACATTCAGC AGGAGGAACT TACAGAGGAT
    GTAGATGAGA TTGACCATGC AGAAATGGAG CTTAGGAGAG GCCAGATTTT GTGGTTCAGA
    GGCCTAAACC GAATACAGAC TCAGATTAAA GTTGTGAATG CGTTCCGAAG CTCTCTCTAC
    GAGGGGCTAG AGAAGCCACA GATCCGCAGT GCCATCCATA GCTTCATGAC CCACCCAGAG
    TTCACTCCAC GGGAGGCAGG ACCTGCCCCC CAATTCGAAG ACAGCGACAG CGAGGGTGAG
    GAATCTAAGG GTCTCTCAAA GGCGCATAAA GATCAAACCC ATAATCACAA CAACAATTCT
    TCTGTTGACC CACATGCATT GGATATCACA GTGTCTCTAT CTGATAGCCC ATTGCATAGC
    ATGGAAACCT CCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012826089.1
    CDS773..4390
    Translation

    Target ORF information:

    RefSeq Version XM_012970635.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis plasma membrane calcium-transporting ATPase 4 (LOC100488346), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970635.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATA ACTCCACAGA CCATCATTCT GCTAACATTG TAAATGAAGG CAGCTATGAA 
    GGAGATTTTG GCTGCACAAT AATGGACTTA CGGGAGCTTA TGGAATTACG CAGTGCAGAG
    GCAGTCAACC GTATCAGGGA TACTTATGGA GGGGTTCACA ACATCTGCCG CAGGCTAAAG
    ACTTCACCTG TAGAAGGTTT GTCAGGTAAC CCCTCAGACC TCGAGAGGAG GAGACAAATA
    TTTGGTAAAA ACTTCATCCC TCCAAAAAAA GCCAAAACTT TCTTGCAGCT GGTGTGGGAA
    GCACTGCAGG ACGTTACCTT AATTATCCTG GAAATAGCAG CCATAATATC TCTGGGTCTT
    TCTTTCTACC ACCCACCAGG AGGAGATAAT GAATTATGTG GGGAAGCTGC TGCAGGAGTG
    GAGGATGAAG GTGAGGCTCA AGCTGGATGG ATTGAAGGGG CAGCCATCTT ATTCTCGGTC
    ATCATAGTTG TGCTTGTTAC CGCCTTCAAT GACTGGAGCA AAGAGAAGCA ATTTCGAGGC
    CTACAGAGCC GAATTGAGCA GGAGCAGAAA TTCACTGTTA TCCGCAAAGG ACAGGTTATC
    CAAATCCCTG TAGCAGAGCT AGTAGTCGGT GATATTGCAC AAATAAAATA TGGTGACCTC
    CTTCCTGCTG ATGGGATATT GATTCAAGGC AATGACTTAA AAATTGATGA AAGCTCTCTA
    ACTGGGGAGA GCGATCAGGT GAAAAAATCA TTAGAGAAAG ACCCCATGCT TTTGTCAGGA
    ACACACGTAA TGGAAGGGTC TGGACGTATG GTAGTCACAG CAGTAGGTGT CAACTCTCAA
    ACTGGCATAA TATTCACTTT GCTAGGTGCA AATGAGGGAG GTGAAGAGGA AAAAAAATCC
    AAAAAAGGTA AAAAACCAGG ACCCTCAGAA AATCGCAACA AAGCCAAAGC TCAAGATGGT
    GTTGCATTAG AGATCCAACC ACTGAAAAGT GAAGAGGGCG TAGAGTGTGA AGAGAAAGAG
    AAAAAGGTTG TGAAAGTGCC AAAGAAAGAA AAGTCTGTCC TTCAGGGGAA GCTAACTCGG
    CTGGCAGTGC AAATTGGAAA AGCAGGTCTC ATTATGTCAG CCATCACAGT GATCATTTTG
    GTGCTCTATT TTGTCATTTA TACATTTGGA GTGTTAGGTC GCCCATGGTT GGCAGAATGC
    ACTCCCATCT ACATCCAGTA CTTTGTCAAA TTTTTCATTA TTGGTGTCAC TGTGTTGGTG
    GTGGCTGTCC CTGAGGGGCT GCCTCTCGCT GTAACAATAT CTTTGGCCTA CTCTGTAAAG
    AAAATGATGA AAGACAATAA TCTTGTACGT CACTTGGATG CCTGTGAAAC TATGGGCAAT
    GCAACAGCCA TATGCTCTGA TAAAACTGGA ACGCTCACTA TGAACCGAAT GACTGTAGTA
    CAGGCATTTG TTGGTGGTAC TCATTATAGG CAGATTCCTG ATCCGGAGGC ACTGAACACA
    AAAATTCTTG ATCTTATAGT AAATGGGATT TCAGTCAACT CAGCATACAC CTCCAAGATC
    CTGCCCCCTG AAAAGGAGGG AGGTCTCCCC AGGCAGGTGG GTAACAAGAC AGAATGTGCG
    CTACTGGGAT TTGTTTTGGA CCTGAAGCAA GATTATCAGA CAGTGCGAAA CGAAATTCCT
    GAAGAGAACC TCTACAAAGT TTATACGTTC AATTCTGTGC GAAAATCTAT GAGCACAGTG
    TTGTGTGACT CTTCTGGAAA ATTCCGCATG TACAGCAAAG GGGCCTCTGA AATCATCTTG
    CGCAAATGCA CCCGAATCCT GGACCAAGGA GGAGATGTGT GCCCCTTTAA AGCAAAGGAC
    CGGGATGAGA TGGTAAAGAA AGTAATTGAA CCTATGGCAT GCGATGGCTT GCGCACAATC
    TGCCTAGCAT ATCGTGACTT CCCTTCAGAT AGTGAGCCTA ATTGGGATAA TGAGGGCGAC
    ATCCTCTCTG ACCTTATTTG CATTGCTGTA GTAGGAATAG AAGATCCTGT TCGACCAGAG
    GTGCCTGAAG CAATTCAAAA GTGCCAGCGT GCAGGTATCA CAGTGCGCAT GGTTACTGGG
    GACAATATAA ACACAGCTCG AGCAATAGCT ACCAAGTGTG GTATCCTGCA GCCAGGAGAA
    GACTTCTTGT GCTTGGAGGG AAAAGAATTC AACACACTGA TCCGAAATGA AAAGGGAGAG
    GTAGAACAGG ATAAATTGGA CAAGGTTTGG CCGAGACTTC GTGTTTTGGC CCGGTCATCA
    CCTACAGATA AACATACTCT GGTAAAAGGC ATCATTGACA GCACAGTGGC AGAGCGAAGA
    CAGGTGGTGG CTGTGACTGG AGATGGAACA AACGATGGAC CTGCCCTGAA GAAAGCAGAT
    GTGGGATTTG CAATGGGCAT AGCTGGCACA GATGTTGCAA AGGAGGCTTC TGATATTATC
    TTGACAGACG ACAATTTCAC CAGCATTGTA AAGGCTGTAA TGTGGGGCCG CAATGTATAC
    GACAGTATTT CGAAGTTCCT GCAGTTCCAG CTCACCGTAA ATGTTGTGGC AGTAATTGTG
    GCATTTACAG GAGCCTGCAT AACTCAGGAT TCACCACTGA AAGCTGTGCA GATGCTGTGG
    GTCAACCTTA TCATGGATAC ATTTGCATCT TTAGCTTTGG CGACTGAGCC TCCTACTGAT
    TCGCTTCTTC TACGCAAACC ATATGGGCGC AACAAACCCC TGATCTCACG TACAATGATG
    AAAAACATAT TGGGACATGC TGTTTATCAG CTTACCATCA TATTTACATT GCTTTTTGCA
    GGTGAGAAGT TTTTTGACAT TGACAGTGGG AGAAATGTAC CCCTGCATTC TCCACCTTCT
    GAACATTATA CAATTGTCTT CAACACCTTT GTGATGATGC AGCTTTTTAA TGAGATCAAT
    GCCCGCAAGA TCCACGGTGA ACGTAATGTC TTTGAGAACA TCTTCCGGAA CCCCATCTTT
    TGTGCAGTTG TGCTGGGCAC TTTTGGAGCT CAGATAATAA TTGTGGAGTT TGGAGGAAAG
    CCATTCAGTT GCTCTGGCCT TACTTTGTCA CAGTGGTTCT GGTGTATCTT CATTGGGGTT
    GGAGAGCTGT TGTGGGGACA GATGATTTGC ACAATACCCA CCAGCCGACT CAAGTTCCTA
    AAAGAGGCTG GCCATGGGCC ACTGAAGGAG GACATTCAGC AGGAGGAACT TACAGAGGAT
    GTAGATGAGA TTGACCATGC AGAAATGGAG CTTAGGAGAG GCCAGATTTT GTGGTTCAGA
    GGCCTAAACC GAATACAGAC TCAGATTAAA GTTGTGAATG CGTTCCGAAG CTCTCTCTAC
    GAGGGGCTAG AGAAGCCACA GATCCGCAGT GCCATCCATA GCTTCATGAC CCACCCAGAG
    TTCACTCCAC GGGAGGCAGG ACCTGCCCCC CAATTCGAAG ACAGCGACAG CGAGGGTGAG
    GAATCTAAGG GTCTCTCAAA GGCGCATAAA GATCAAACCC ATAATCACAA CAACAATTCT
    TCTGTTGACC CACATGCATT GGATATCACA GTGTCTCTAT CTGATAGCCC ATTGCATAGC
    ATGGAAACCT CCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa03801
    Clone ID Related Accession (Same CDS sequence) XM_004910677.3 , XM_012970635.2 , XM_012970637.2 , XM_012970637.3 , XM_004910677.4 , XM_012970635.3
    Accession Version XM_012970637.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3618bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product plasma membrane calcium-transporting ATPase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970637.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATA ACTCCACAGA CCATCATTCT GCTAACATTG TAAATGAAGG CAGCTATGAA 
    GGAGATTTTG GCTGCACAAT AATGGACTTA CGGGAGCTTA TGGAATTACG CAGTGCAGAG
    GCAGTCAACC GTATCAGGGA TACTTATGGA GGGGTTCACA ACATCTGCCG CAGGCTAAAG
    ACTTCACCTG TAGAAGGTTT GTCAGGTAAC CCCTCAGACC TCGAGAGGAG GAGACAAATA
    TTTGGTAAAA ACTTCATCCC TCCAAAAAAA GCCAAAACTT TCTTGCAGCT GGTGTGGGAA
    GCACTGCAGG ACGTTACCTT AATTATCCTG GAAATAGCAG CCATAATATC TCTGGGTCTT
    TCTTTCTACC ACCCACCAGG AGGAGATAAT GAATTATGTG GGGAAGCTGC TGCAGGAGTG
    GAGGATGAAG GTGAGGCTCA AGCTGGATGG ATTGAAGGGG CAGCCATCTT ATTCTCGGTC
    ATCATAGTTG TGCTTGTTAC CGCCTTCAAT GACTGGAGCA AAGAGAAGCA ATTTCGAGGC
    CTACAGAGCC GAATTGAGCA GGAGCAGAAA TTCACTGTTA TCCGCAAAGG ACAGGTTATC
    CAAATCCCTG TAGCAGAGCT AGTAGTCGGT GATATTGCAC AAATAAAATA TGGTGACCTC
    CTTCCTGCTG ATGGGATATT GATTCAAGGC AATGACTTAA AAATTGATGA AAGCTCTCTA
    ACTGGGGAGA GCGATCAGGT GAAAAAATCA TTAGAGAAAG ACCCCATGCT TTTGTCAGGA
    ACACACGTAA TGGAAGGGTC TGGACGTATG GTAGTCACAG CAGTAGGTGT CAACTCTCAA
    ACTGGCATAA TATTCACTTT GCTAGGTGCA AATGAGGGAG GTGAAGAGGA AAAAAAATCC
    AAAAAAGGTA AAAAACCAGG ACCCTCAGAA AATCGCAACA AAGCCAAAGC TCAAGATGGT
    GTTGCATTAG AGATCCAACC ACTGAAAAGT GAAGAGGGCG TAGAGTGTGA AGAGAAAGAG
    AAAAAGGTTG TGAAAGTGCC AAAGAAAGAA AAGTCTGTCC TTCAGGGGAA GCTAACTCGG
    CTGGCAGTGC AAATTGGAAA AGCAGGTCTC ATTATGTCAG CCATCACAGT GATCATTTTG
    GTGCTCTATT TTGTCATTTA TACATTTGGA GTGTTAGGTC GCCCATGGTT GGCAGAATGC
    ACTCCCATCT ACATCCAGTA CTTTGTCAAA TTTTTCATTA TTGGTGTCAC TGTGTTGGTG
    GTGGCTGTCC CTGAGGGGCT GCCTCTCGCT GTAACAATAT CTTTGGCCTA CTCTGTAAAG
    AAAATGATGA AAGACAATAA TCTTGTACGT CACTTGGATG CCTGTGAAAC TATGGGCAAT
    GCAACAGCCA TATGCTCTGA TAAAACTGGA ACGCTCACTA TGAACCGAAT GACTGTAGTA
    CAGGCATTTG TTGGTGGTAC TCATTATAGG CAGATTCCTG ATCCGGAGGC ACTGAACACA
    AAAATTCTTG ATCTTATAGT AAATGGGATT TCAGTCAACT CAGCATACAC CTCCAAGATC
    CTGCCCCCTG AAAAGGAGGG AGGTCTCCCC AGGCAGGTGG GTAACAAGAC AGAATGTGCG
    CTACTGGGAT TTGTTTTGGA CCTGAAGCAA GATTATCAGA CAGTGCGAAA CGAAATTCCT
    GAAGAGAACC TCTACAAAGT TTATACGTTC AATTCTGTGC GAAAATCTAT GAGCACAGTG
    TTGTGTGACT CTTCTGGAAA ATTCCGCATG TACAGCAAAG GGGCCTCTGA AATCATCTTG
    CGCAAATGCA CCCGAATCCT GGACCAAGGA GGAGATGTGT GCCCCTTTAA AGCAAAGGAC
    CGGGATGAGA TGGTAAAGAA AGTAATTGAA CCTATGGCAT GCGATGGCTT GCGCACAATC
    TGCCTAGCAT ATCGTGACTT CCCTTCAGAT AGTGAGCCTA ATTGGGATAA TGAGGGCGAC
    ATCCTCTCTG ACCTTATTTG CATTGCTGTA GTAGGAATAG AAGATCCTGT TCGACCAGAG
    GTGCCTGAAG CAATTCAAAA GTGCCAGCGT GCAGGTATCA CAGTGCGCAT GGTTACTGGG
    GACAATATAA ACACAGCTCG AGCAATAGCT ACCAAGTGTG GTATCCTGCA GCCAGGAGAA
    GACTTCTTGT GCTTGGAGGG AAAAGAATTC AACACACTGA TCCGAAATGA AAAGGGAGAG
    GTAGAACAGG ATAAATTGGA CAAGGTTTGG CCGAGACTTC GTGTTTTGGC CCGGTCATCA
    CCTACAGATA AACATACTCT GGTAAAAGGC ATCATTGACA GCACAGTGGC AGAGCGAAGA
    CAGGTGGTGG CTGTGACTGG AGATGGAACA AACGATGGAC CTGCCCTGAA GAAAGCAGAT
    GTGGGATTTG CAATGGGCAT AGCTGGCACA GATGTTGCAA AGGAGGCTTC TGATATTATC
    TTGACAGACG ACAATTTCAC CAGCATTGTA AAGGCTGTAA TGTGGGGCCG CAATGTATAC
    GACAGTATTT CGAAGTTCCT GCAGTTCCAG CTCACCGTAA ATGTTGTGGC AGTAATTGTG
    GCATTTACAG GAGCCTGCAT AACTCAGGAT TCACCACTGA AAGCTGTGCA GATGCTGTGG
    GTCAACCTTA TCATGGATAC ATTTGCATCT TTAGCTTTGG CGACTGAGCC TCCTACTGAT
    TCGCTTCTTC TACGCAAACC ATATGGGCGC AACAAACCCC TGATCTCACG TACAATGATG
    AAAAACATAT TGGGACATGC TGTTTATCAG CTTACCATCA TATTTACATT GCTTTTTGCA
    GGTGAGAAGT TTTTTGACAT TGACAGTGGG AGAAATGTAC CCCTGCATTC TCCACCTTCT
    GAACATTATA CAATTGTCTT CAACACCTTT GTGATGATGC AGCTTTTTAA TGAGATCAAT
    GCCCGCAAGA TCCACGGTGA ACGTAATGTC TTTGAGAACA TCTTCCGGAA CCCCATCTTT
    TGTGCAGTTG TGCTGGGCAC TTTTGGAGCT CAGATAATAA TTGTGGAGTT TGGAGGAAAG
    CCATTCAGTT GCTCTGGCCT TACTTTGTCA CAGTGGTTCT GGTGTATCTT CATTGGGGTT
    GGAGAGCTGT TGTGGGGACA GATGATTTGC ACAATACCCA CCAGCCGACT CAAGTTCCTA
    AAAGAGGCTG GCCATGGGCC ACTGAAGGAG GACATTCAGC AGGAGGAACT TACAGAGGAT
    GTAGATGAGA TTGACCATGC AGAAATGGAG CTTAGGAGAG GCCAGATTTT GTGGTTCAGA
    GGCCTAAACC GAATACAGAC TCAGATTAAA GTTGTGAATG CGTTCCGAAG CTCTCTCTAC
    GAGGGGCTAG AGAAGCCACA GATCCGCAGT GCCATCCATA GCTTCATGAC CCACCCAGAG
    TTCACTCCAC GGGAGGCAGG ACCTGCCCCC CAATTCGAAG ACAGCGACAG CGAGGGTGAG
    GAATCTAAGG GTCTCTCAAA GGCGCATAAA GATCAAACCC ATAATCACAA CAACAATTCT
    TCTGTTGACC CACATGCATT GGATATCACA GTGTCTCTAT CTGATAGCCC ATTGCATAGC
    ATGGAAACCT CCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012826091.1
    CDS568..4185
    Translation

    Target ORF information:

    RefSeq Version XM_012970637.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis plasma membrane calcium-transporting ATPase 4 (LOC100488346), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970637.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATA ACTCCACAGA CCATCATTCT GCTAACATTG TAAATGAAGG CAGCTATGAA 
    GGAGATTTTG GCTGCACAAT AATGGACTTA CGGGAGCTTA TGGAATTACG CAGTGCAGAG
    GCAGTCAACC GTATCAGGGA TACTTATGGA GGGGTTCACA ACATCTGCCG CAGGCTAAAG
    ACTTCACCTG TAGAAGGTTT GTCAGGTAAC CCCTCAGACC TCGAGAGGAG GAGACAAATA
    TTTGGTAAAA ACTTCATCCC TCCAAAAAAA GCCAAAACTT TCTTGCAGCT GGTGTGGGAA
    GCACTGCAGG ACGTTACCTT AATTATCCTG GAAATAGCAG CCATAATATC TCTGGGTCTT
    TCTTTCTACC ACCCACCAGG AGGAGATAAT GAATTATGTG GGGAAGCTGC TGCAGGAGTG
    GAGGATGAAG GTGAGGCTCA AGCTGGATGG ATTGAAGGGG CAGCCATCTT ATTCTCGGTC
    ATCATAGTTG TGCTTGTTAC CGCCTTCAAT GACTGGAGCA AAGAGAAGCA ATTTCGAGGC
    CTACAGAGCC GAATTGAGCA GGAGCAGAAA TTCACTGTTA TCCGCAAAGG ACAGGTTATC
    CAAATCCCTG TAGCAGAGCT AGTAGTCGGT GATATTGCAC AAATAAAATA TGGTGACCTC
    CTTCCTGCTG ATGGGATATT GATTCAAGGC AATGACTTAA AAATTGATGA AAGCTCTCTA
    ACTGGGGAGA GCGATCAGGT GAAAAAATCA TTAGAGAAAG ACCCCATGCT TTTGTCAGGA
    ACACACGTAA TGGAAGGGTC TGGACGTATG GTAGTCACAG CAGTAGGTGT CAACTCTCAA
    ACTGGCATAA TATTCACTTT GCTAGGTGCA AATGAGGGAG GTGAAGAGGA AAAAAAATCC
    AAAAAAGGTA AAAAACCAGG ACCCTCAGAA AATCGCAACA AAGCCAAAGC TCAAGATGGT
    GTTGCATTAG AGATCCAACC ACTGAAAAGT GAAGAGGGCG TAGAGTGTGA AGAGAAAGAG
    AAAAAGGTTG TGAAAGTGCC AAAGAAAGAA AAGTCTGTCC TTCAGGGGAA GCTAACTCGG
    CTGGCAGTGC AAATTGGAAA AGCAGGTCTC ATTATGTCAG CCATCACAGT GATCATTTTG
    GTGCTCTATT TTGTCATTTA TACATTTGGA GTGTTAGGTC GCCCATGGTT GGCAGAATGC
    ACTCCCATCT ACATCCAGTA CTTTGTCAAA TTTTTCATTA TTGGTGTCAC TGTGTTGGTG
    GTGGCTGTCC CTGAGGGGCT GCCTCTCGCT GTAACAATAT CTTTGGCCTA CTCTGTAAAG
    AAAATGATGA AAGACAATAA TCTTGTACGT CACTTGGATG CCTGTGAAAC TATGGGCAAT
    GCAACAGCCA TATGCTCTGA TAAAACTGGA ACGCTCACTA TGAACCGAAT GACTGTAGTA
    CAGGCATTTG TTGGTGGTAC TCATTATAGG CAGATTCCTG ATCCGGAGGC ACTGAACACA
    AAAATTCTTG ATCTTATAGT AAATGGGATT TCAGTCAACT CAGCATACAC CTCCAAGATC
    CTGCCCCCTG AAAAGGAGGG AGGTCTCCCC AGGCAGGTGG GTAACAAGAC AGAATGTGCG
    CTACTGGGAT TTGTTTTGGA CCTGAAGCAA GATTATCAGA CAGTGCGAAA CGAAATTCCT
    GAAGAGAACC TCTACAAAGT TTATACGTTC AATTCTGTGC GAAAATCTAT GAGCACAGTG
    TTGTGTGACT CTTCTGGAAA ATTCCGCATG TACAGCAAAG GGGCCTCTGA AATCATCTTG
    CGCAAATGCA CCCGAATCCT GGACCAAGGA GGAGATGTGT GCCCCTTTAA AGCAAAGGAC
    CGGGATGAGA TGGTAAAGAA AGTAATTGAA CCTATGGCAT GCGATGGCTT GCGCACAATC
    TGCCTAGCAT ATCGTGACTT CCCTTCAGAT AGTGAGCCTA ATTGGGATAA TGAGGGCGAC
    ATCCTCTCTG ACCTTATTTG CATTGCTGTA GTAGGAATAG AAGATCCTGT TCGACCAGAG
    GTGCCTGAAG CAATTCAAAA GTGCCAGCGT GCAGGTATCA CAGTGCGCAT GGTTACTGGG
    GACAATATAA ACACAGCTCG AGCAATAGCT ACCAAGTGTG GTATCCTGCA GCCAGGAGAA
    GACTTCTTGT GCTTGGAGGG AAAAGAATTC AACACACTGA TCCGAAATGA AAAGGGAGAG
    GTAGAACAGG ATAAATTGGA CAAGGTTTGG CCGAGACTTC GTGTTTTGGC CCGGTCATCA
    CCTACAGATA AACATACTCT GGTAAAAGGC ATCATTGACA GCACAGTGGC AGAGCGAAGA
    CAGGTGGTGG CTGTGACTGG AGATGGAACA AACGATGGAC CTGCCCTGAA GAAAGCAGAT
    GTGGGATTTG CAATGGGCAT AGCTGGCACA GATGTTGCAA AGGAGGCTTC TGATATTATC
    TTGACAGACG ACAATTTCAC CAGCATTGTA AAGGCTGTAA TGTGGGGCCG CAATGTATAC
    GACAGTATTT CGAAGTTCCT GCAGTTCCAG CTCACCGTAA ATGTTGTGGC AGTAATTGTG
    GCATTTACAG GAGCCTGCAT AACTCAGGAT TCACCACTGA AAGCTGTGCA GATGCTGTGG
    GTCAACCTTA TCATGGATAC ATTTGCATCT TTAGCTTTGG CGACTGAGCC TCCTACTGAT
    TCGCTTCTTC TACGCAAACC ATATGGGCGC AACAAACCCC TGATCTCACG TACAATGATG
    AAAAACATAT TGGGACATGC TGTTTATCAG CTTACCATCA TATTTACATT GCTTTTTGCA
    GGTGAGAAGT TTTTTGACAT TGACAGTGGG AGAAATGTAC CCCTGCATTC TCCACCTTCT
    GAACATTATA CAATTGTCTT CAACACCTTT GTGATGATGC AGCTTTTTAA TGAGATCAAT
    GCCCGCAAGA TCCACGGTGA ACGTAATGTC TTTGAGAACA TCTTCCGGAA CCCCATCTTT
    TGTGCAGTTG TGCTGGGCAC TTTTGGAGCT CAGATAATAA TTGTGGAGTT TGGAGGAAAG
    CCATTCAGTT GCTCTGGCCT TACTTTGTCA CAGTGGTTCT GGTGTATCTT CATTGGGGTT
    GGAGAGCTGT TGTGGGGACA GATGATTTGC ACAATACCCA CCAGCCGACT CAAGTTCCTA
    AAAGAGGCTG GCCATGGGCC ACTGAAGGAG GACATTCAGC AGGAGGAACT TACAGAGGAT
    GTAGATGAGA TTGACCATGC AGAAATGGAG CTTAGGAGAG GCCAGATTTT GTGGTTCAGA
    GGCCTAAACC GAATACAGAC TCAGATTAAA GTTGTGAATG CGTTCCGAAG CTCTCTCTAC
    GAGGGGCTAG AGAAGCCACA GATCCGCAGT GCCATCCATA GCTTCATGAC CCACCCAGAG
    TTCACTCCAC GGGAGGCAGG ACCTGCCCCC CAATTCGAAG ACAGCGACAG CGAGGGTGAG
    GAATCTAAGG GTCTCTCAAA GGCGCATAAA GATCAAACCC ATAATCACAA CAACAATTCT
    TCTGTTGACC CACATGCATT GGATATCACA GTGTCTCTAT CTGATAGCCC ATTGCATAGC
    ATGGAAACCT CCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa03805
    Clone ID Related Accession (Same CDS sequence) XM_002932973.4 , XM_002932973.5
    Accession Version XM_002932973.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3507bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product plasma membrane calcium-transporting ATPase 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002932973.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCAAATA ACTCCACAGA CCATCATTCT GCTAACATTG TAAATGAAGG CAGCTATGAA 
    GGAGATTTTG GCTGCACAAT AATGGACTTA CGGGAGCTTA TGGAATTACG CAGTGCAGAG
    GCAGTCAACC GTATCAGGGA TACTTATGGA GGGGTTCACA ACATCTGCCG CAGGCTAAAG
    ACTTCACCTG TAGAAGGTTT GTCAGGTAAC CCCTCAGACC TCGAGAGGAG GAGACAAATA
    TTTGGTAAAA ACTTCATCCC TCCAAAAAAA GCCAAAACTT TCTTGCAGCT GGTGTGGGAA
    GCACTGCAGG ACGTTACCTT AATTATCCTG GAAATAGCAG CCATAATATC TCTGGGTCTT
    TCTTTCTACC ACCCACCAGG AGGAGATAAT GAATTATGTG GGGAAGCTGC TGCAGGAGTG
    GAGGATGAAG GTGAGGCTCA AGCTGGATGG ATTGAAGGGG CAGCCATCTT ATTCTCGGTC
    ATCATAGTTG TGCTTGTTAC CGCCTTCAAT GACTGGAGCA AAGAGAAGCA ATTTCGAGGC
    CTACAGAGCC GAATTGAGCA GGAGCAGAAA TTCACTGTTA TCCGCAAAGG ACAGGTTATC
    CAAATCCCTG TAGCAGAGCT AGTAGTCGGT GATATTGCAC AAATAAAATA TGGTGACCTC
    CTTCCTGCTG ATGGGATATT GATTCAAGGC AATGACTTAA AAATTGATGA AAGCTCTCTA
    ACTGGGGAGA GCGATCAGGT GAAAAAATCA TTAGAGAAAG ACCCCATGCT TTTGTCAGGA
    ACACACGTAA TGGAAGGGTC TGGACGTATG GTAGTCACAG CAGTAGGTGT CAACTCTCAA
    ACTGGCATAA TATTCACTTT GCTAGGTGCA AATGAGGGAG GTGAAGAGGA AAAAAAATCC
    AAAAAAGGTA AAAAACCAGG ACCCTCAGAA AATCGCAACA AAGCCAAAGC TCAAGATGGT
    GTTGCATTAG AGATCCAACC ACTGAAAAGT GAAGAGGGCG TAGAGTGTGA AGAGAAAGAG
    AAAAAGGTTG TGAAAGTGCC AAAGAAAGAA AAGTCTGTCC TTCAGGGGAA GCTAACTCGG
    CTGGCAGTGC AAATTGGAAA AGCAGGTCTC ATTATGTCAG CCATCACAGT GATCATTTTG
    GTGCTCTATT TTGTCATTTA TACATTTGGA GTGTTAGGTC GCCCATGGTT GGCAGAATGC
    ACTCCCATCT ACATCCAGTA CTTTGTCAAA TTTTTCATTA TTGGTGTCAC TGTGTTGGTG
    GTGGCTGTCC CTGAGGGGCT GCCTCTCGCT GTAACAATAT CTTTGGCCTA CTCTGTAAAG
    AAAATGATGA AAGACAATAA TCTTGTACGT CACTTGGATG CCTGTGAAAC TATGGGCAAT
    GCAACAGCCA TATGCTCTGA TAAAACTGGA ACGCTCACTA TGAACCGAAT GACTGTAGTA
    CAGGCATTTG TTGGTGGTAC TCATTATAGG CAGATTCCTG ATCCGGAGGC ACTGAACACA
    AAAATTCTTG ATCTTATAGT AAATGGGATT TCAGTCAACT CAGCATACAC CTCCAAGATC
    CTGCCCCCTG AAAAGGAGGG AGGTCTCCCC AGGCAGGTGG GTAACAAGAC AGAATGTGCG
    CTACTGGGAT TTGTTTTGGA CCTGAAGCAA GATTATCAGA CAGTGCGAAA CGAAATTCCT
    GAAGAGAACC TCTACAAAGT TTATACGTTC AATTCTGTGC GAAAATCTAT GAGCACAGTG
    TTGTGTGACT CTTCTGGAAA ATTCCGCATG TACAGCAAAG GGGCCTCTGA AATCATCTTG
    CGCAAATGCA CCCGAATCCT GGACCAAGGA GGAGATGTGT GCCCCTTTAA AGCAAAGGAC
    CGGGATGAGA TGGTAAAGAA AGTAATTGAA CCTATGGCAT GCGATGGCTT GCGCACAATC
    TGCCTAGCAT ATCGTGACTT CCCTTCAGAT AGTGAGCCTA ATTGGGATAA TGAGGGCGAC
    ATCCTCTCTG ACCTTATTTG CATTGCTGTA GTAGGAATAG AAGATCCTGT TCGACCAGAG
    GTGCCTGAAG CAATTCAAAA GTGCCAGCGT GCAGGTATCA CAGTGCGCAT GGTTACTGGG
    GACAATATAA ACACAGCTCG AGCAATAGCT ACCAAGTGTG GTATCCTGCA GCCAGGAGAA
    GACTTCTTGT GCTTGGAGGG AAAAGAATTC AACACACTGA TCCGAAATGA AAAGGGAGAG
    GTAGAACAGG ATAAATTGGA CAAGGTTTGG CCGAGACTTC GTGTTTTGGC CCGGTCATCA
    CCTACAGATA AACATACTCT GGTAAAAGGC ATCATTGACA GCACAGTGGC AGAGCGAAGA
    CAGGTGGTGG CTGTGACTGG AGATGGAACA AACGATGGAC CTGCCCTGAA GAAAGCAGAT
    GTGGGATTTG CAATGGGCAT AGCTGGCACA GATGTTGCAA AGGAGGCTTC TGATATTATC
    TTGACAGACG ACAATTTCAC CAGCATTGTA AAGGCTGTAA TGTGGGGCCG CAATGTATAC
    GACAGTATTT CGAAGTTCCT GCAGTTCCAG CTCACCGTAA ATGTTGTGGC AGTAATTGTG
    GCATTTACAG GAGCCTGCAT AACTCAGGAT TCACCACTGA AAGCTGTGCA GATGCTGTGG
    GTCAACCTTA TCATGGATAC ATTTGCATCT TTAGCTTTGG CGACTGAGCC TCCTACTGAT
    TCGCTTCTTC TACGCAAACC ATATGGGCGC AACAAACCCC TGATCTCACG TACAATGATG
    AAAAACATAT TGGGACATGC TGTTTATCAG CTTACCATCA TATTTACATT GCTTTTTGCA
    GGTGAGAAGT TTTTTGACAT TGACAGTGGG AGAAATGTAC CCCTGCATTC TCCACCTTCT
    GAACATTATA CAATTGTCTT CAACACCTTT GTGATGATGC AGCTTTTTAA TGAGATCAAT
    GCCCGCAAGA TCCACGGTGA ACGTAATGTC TTTGAGAACA TCTTCCGGAA CCCCATCTTT
    TGTGCAGTTG TGCTGGGCAC TTTTGGAGCT CAGATAATAA TTGTGGAGTT TGGAGGAAAG
    CCATTCAGTT GCTCTGGCCT TACTTTGTCA CAGTGGTTCT GGTGTATCTT CATTGGGGTT
    GGAGAGCTGT TGTGGGGACA GATGATTTGC ACAATACCCA CCAGCCGACT CAAGTTCCTA
    AAAGAGGCTG GCCATGGGCC ACTGAAGGAG GACATTCAGC AGGAGGAACT TACAGAGGAT
    GTAGATGAGA TTGACCATGC AGAAATGGAG CTTAGGAGAG GCCAGATTTT GTGGTTCAGA
    GGCCTAAACC GAATACAGAC TCAGATGGAC GTAGTTTATA CATTCCAGAC GGGAGGTACC
    TCTTTACAAG GGGCTCTGAG GCGCCAGCCT TCCACCATTA GCCAGCACCT TGATGCTAAA
    CATGCCTCTA GCCCAACACA CGTAGCTCTG TCCTCTACTA CCTCTCCCCC AGCCACTTCT
    GCTGCTGCTG CTTCTGTGGG CAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002933019.1
    CDS696..4202
    Translation

    Target ORF information:

    RefSeq Version XM_002932973.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis plasma membrane calcium-transporting ATPase 4 (LOC100488346), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002932973.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCAAATA ACTCCACAGA CCATCATTCT GCTAACATTG TAAATGAAGG CAGCTATGAA 
    GGAGATTTTG GCTGCACAAT AATGGACTTA CGGGAGCTTA TGGAATTACG CAGTGCAGAG
    GCAGTCAACC GTATCAGGGA TACTTATGGA GGGGTTCACA ACATCTGCCG CAGGCTAAAG
    ACTTCACCTG TAGAAGGTTT GTCAGGTAAC CCCTCAGACC TCGAGAGGAG GAGACAAATA
    TTTGGTAAAA ACTTCATCCC TCCAAAAAAA GCCAAAACTT TCTTGCAGCT GGTGTGGGAA
    GCACTGCAGG ACGTTACCTT AATTATCCTG GAAATAGCAG CCATAATATC TCTGGGTCTT
    TCTTTCTACC ACCCACCAGG AGGAGATAAT GAATTATGTG GGGAAGCTGC TGCAGGAGTG
    GAGGATGAAG GTGAGGCTCA AGCTGGATGG ATTGAAGGGG CAGCCATCTT ATTCTCGGTC
    ATCATAGTTG TGCTTGTTAC CGCCTTCAAT GACTGGAGCA AAGAGAAGCA ATTTCGAGGC
    CTACAGAGCC GAATTGAGCA GGAGCAGAAA TTCACTGTTA TCCGCAAAGG ACAGGTTATC
    CAAATCCCTG TAGCAGAGCT AGTAGTCGGT GATATTGCAC AAATAAAATA TGGTGACCTC
    CTTCCTGCTG ATGGGATATT GATTCAAGGC AATGACTTAA AAATTGATGA AAGCTCTCTA
    ACTGGGGAGA GCGATCAGGT GAAAAAATCA TTAGAGAAAG ACCCCATGCT TTTGTCAGGA
    ACACACGTAA TGGAAGGGTC TGGACGTATG GTAGTCACAG CAGTAGGTGT CAACTCTCAA
    ACTGGCATAA TATTCACTTT GCTAGGTGCA AATGAGGGAG GTGAAGAGGA AAAAAAATCC
    AAAAAAGGTA AAAAACCAGG ACCCTCAGAA AATCGCAACA AAGCCAAAGC TCAAGATGGT
    GTTGCATTAG AGATCCAACC ACTGAAAAGT GAAGAGGGCG TAGAGTGTGA AGAGAAAGAG
    AAAAAGGTTG TGAAAGTGCC AAAGAAAGAA AAGTCTGTCC TTCAGGGGAA GCTAACTCGG
    CTGGCAGTGC AAATTGGAAA AGCAGGTCTC ATTATGTCAG CCATCACAGT GATCATTTTG
    GTGCTCTATT TTGTCATTTA TACATTTGGA GTGTTAGGTC GCCCATGGTT GGCAGAATGC
    ACTCCCATCT ACATCCAGTA CTTTGTCAAA TTTTTCATTA TTGGTGTCAC TGTGTTGGTG
    GTGGCTGTCC CTGAGGGGCT GCCTCTCGCT GTAACAATAT CTTTGGCCTA CTCTGTAAAG
    AAAATGATGA AAGACAATAA TCTTGTACGT CACTTGGATG CCTGTGAAAC TATGGGCAAT
    GCAACAGCCA TATGCTCTGA TAAAACTGGA ACGCTCACTA TGAACCGAAT GACTGTAGTA
    CAGGCATTTG TTGGTGGTAC TCATTATAGG CAGATTCCTG ATCCGGAGGC ACTGAACACA
    AAAATTCTTG ATCTTATAGT AAATGGGATT TCAGTCAACT CAGCATACAC CTCCAAGATC
    CTGCCCCCTG AAAAGGAGGG AGGTCTCCCC AGGCAGGTGG GTAACAAGAC AGAATGTGCG
    CTACTGGGAT TTGTTTTGGA CCTGAAGCAA GATTATCAGA CAGTGCGAAA CGAAATTCCT
    GAAGAGAACC TCTACAAAGT TTATACGTTC AATTCTGTGC GAAAATCTAT GAGCACAGTG
    TTGTGTGACT CTTCTGGAAA ATTCCGCATG TACAGCAAAG GGGCCTCTGA AATCATCTTG
    CGCAAATGCA CCCGAATCCT GGACCAAGGA GGAGATGTGT GCCCCTTTAA AGCAAAGGAC
    CGGGATGAGA TGGTAAAGAA AGTAATTGAA CCTATGGCAT GCGATGGCTT GCGCACAATC
    TGCCTAGCAT ATCGTGACTT CCCTTCAGAT AGTGAGCCTA ATTGGGATAA TGAGGGCGAC
    ATCCTCTCTG ACCTTATTTG CATTGCTGTA GTAGGAATAG AAGATCCTGT TCGACCAGAG
    GTGCCTGAAG CAATTCAAAA GTGCCAGCGT GCAGGTATCA CAGTGCGCAT GGTTACTGGG
    GACAATATAA ACACAGCTCG AGCAATAGCT ACCAAGTGTG GTATCCTGCA GCCAGGAGAA
    GACTTCTTGT GCTTGGAGGG AAAAGAATTC AACACACTGA TCCGAAATGA AAAGGGAGAG
    GTAGAACAGG ATAAATTGGA CAAGGTTTGG CCGAGACTTC GTGTTTTGGC CCGGTCATCA
    CCTACAGATA AACATACTCT GGTAAAAGGC ATCATTGACA GCACAGTGGC AGAGCGAAGA
    CAGGTGGTGG CTGTGACTGG AGATGGAACA AACGATGGAC CTGCCCTGAA GAAAGCAGAT
    GTGGGATTTG CAATGGGCAT AGCTGGCACA GATGTTGCAA AGGAGGCTTC TGATATTATC
    TTGACAGACG ACAATTTCAC CAGCATTGTA AAGGCTGTAA TGTGGGGCCG CAATGTATAC
    GACAGTATTT CGAAGTTCCT GCAGTTCCAG CTCACCGTAA ATGTTGTGGC AGTAATTGTG
    GCATTTACAG GAGCCTGCAT AACTCAGGAT TCACCACTGA AAGCTGTGCA GATGCTGTGG
    GTCAACCTTA TCATGGATAC ATTTGCATCT TTAGCTTTGG CGACTGAGCC TCCTACTGAT
    TCGCTTCTTC TACGCAAACC ATATGGGCGC AACAAACCCC TGATCTCACG TACAATGATG
    AAAAACATAT TGGGACATGC TGTTTATCAG CTTACCATCA TATTTACATT GCTTTTTGCA
    GGTGAGAAGT TTTTTGACAT TGACAGTGGG AGAAATGTAC CCCTGCATTC TCCACCTTCT
    GAACATTATA CAATTGTCTT CAACACCTTT GTGATGATGC AGCTTTTTAA TGAGATCAAT
    GCCCGCAAGA TCCACGGTGA ACGTAATGTC TTTGAGAACA TCTTCCGGAA CCCCATCTTT
    TGTGCAGTTG TGCTGGGCAC TTTTGGAGCT CAGATAATAA TTGTGGAGTT TGGAGGAAAG
    CCATTCAGTT GCTCTGGCCT TACTTTGTCA CAGTGGTTCT GGTGTATCTT CATTGGGGTT
    GGAGAGCTGT TGTGGGGACA GATGATTTGC ACAATACCCA CCAGCCGACT CAAGTTCCTA
    AAAGAGGCTG GCCATGGGCC ACTGAAGGAG GACATTCAGC AGGAGGAACT TACAGAGGAT
    GTAGATGAGA TTGACCATGC AGAAATGGAG CTTAGGAGAG GCCAGATTTT GTGGTTCAGA
    GGCCTAAACC GAATACAGAC TCAGATGGAC GTAGTTTATA CATTCCAGAC GGGAGGTACC
    TCTTTACAAG GGGCTCTGAG GCGCCAGCCT TCCACCATTA GCCAGCACCT TGATGCTAAA
    CATGCCTCTA GCCCAACACA CGTAGCTCTG TCCTCTACTA CCTCTCCCCC AGCCACTTCT
    GCTGCTGCTG CTTCTGTGGG CAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.