eif2b5 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	eif2b5
Entrez Gene ID	100487780
Full Name	eukaryotic translation initiation factor 2B subunit epsilon
General protein information	Preferred Names eukaryotic translation initiation factor 2B subunit epsilon Names translation initiation factor eIF-2B subunit epsilon eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002935139.4	XP_002935185.2	translation initiation factor eIF-2B subunit epsilon isoform X1
XM_012963364.3	XP_012818818.1	translation initiation factor eIF-2B subunit epsilon isoform X2
XM_012963364.2	XP_012818818.1	translation initiation factor eIF-2B subunit epsilon isoform X2
XM_002935139.5	XP_002935185.3	translation initiation factor eIF-2B subunit epsilon isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	EIF2B5	XP_001139517.1
Gallus gallus (chicken)	EIF2B5	XP_422755.3
Drosophila melanogaster (fruit fly)	eIF2B-epsilon	NP_996329.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000082	XP_001687758.1
Arabidopsis thaliana (thale cress)	AT2G34970	NP_181042.1
	EIF2B5	XP_001104122.1
Danio rerio (zebrafish)	eif2b5	NP_001107069.1
Schizosaccharomyces pombe (fission yeast)	tif225	NP_594285.1
Arabidopsis thaliana (thale cress)	AT4G18300	NP_193564.1
Xenopus tropicalis (tropical clawed frog)	eif2b5	XP_002935185.2
Mus musculus (house mouse)	Eif2b5	NP_758469.1
Saccharomyces cerevisiae (baker's yeast)	GCD6	NP_010497.3
Eremothecium gossypii	AGOS_AGL094W	NP_986572.1
Canis lupus familiaris (dog)	EIF2B5	XP_003640192.1
Bos taurus (cattle)	EIF2B5	XP_002684924.1
Rattus norvegicus (Norway rat)	Eif2b5	NP_620221.2
Kluyveromyces lactis	KLLA0A04235g	XP_451187.1
Neurospora crassa	NCU02414	XP_959596.1
Homo sapiens (human)	EIF2B5	NP_003898.2
Magnaporthe oryzae (rice blast fungus)	MGG_03517	XP_003716402.1
Arabidopsis thaliana (thale cress)	AT3G02270	NP_186876.1
Oryza sativa (rice)	Os02g0812400	NP_001048478.1

Gene Ontology

The following eif2b5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the eif2b5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa10192	XM_002935139.4 Latest version!	Xenopus tropicalis eukaryotic translation initiation factor 2B subunit epsilon (eif2b5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa33981	XM_012963364.3 Latest version!	Xenopus tropicalis eukaryotic translation initiation factor 2B subunit epsilon (eif2b5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OXa58481	XM_002935139.5 Latest version!	Xenopus tropicalis eukaryotic translation initiation factor 2B subunit epsilon (eif2b5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OXa33981	XM_012963364.2	Xenopus tropicalis eukaryotic translation initiation factor 2B subunit epsilon (eif2b5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

eif2b5 ( XM_012963364.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818818.1 Xenopus tropicalis eukaryotic translation initiation factor 2B subunit epsilon (eif2b5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa33981
Clone ID Related Accession (Same CDS sequence)	XM_012963364.3 , XM_012963364.2
Accession Version	XM_012963364.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	translation initiation factor eIF-2B subunit epsilon isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963364.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG ATATTAAGGA GCATCTGAGA AATTCAAAGT GGTGCAGACC TACCAGCCCC 
AATGTGGTAC GGTTTGTGAC CTCTGAGCTT TACAGATCAC TTGGTGATGT TCTGAGAGAT 
GTAGATGCCA AATCTTTAGT AAGATCTGAC TTCATTCTTG TCACTGGTGA TGTGGTGTCC 
AACATCAACA TTGAAGCAGC TTTGGAGCAA CATAGGACAC GGCGGAAGTT AGAAAAAAAT 
GTGTCTGTGA TGACAATGAT CCTTAAGGAG TCCTCTCCAG GTCACCGCAC AAGATGTCAA 
GAGGATGATG TTATAATTGC TATGGACAGT AAGACCAAAC GAGTGCTGCT ATACCAGAAA 
AGTCAGGGCC TGACACGCTT TCACTTTCCA GTGAGCATTT TCCAGAGTAA CACTGATGAA 
ATTGAGCTGC GCCACGATTT ATTGGATTGT CACATCAGCA TTTGCTCTCC ACAAGTTGCA 
GAACTGTTTA CAGATAACTT TGATTACCAG ACTAGGGATG ATTTTGTCCG TGGTATTTTG 
GTTAATGAAG AGATTCTGGG CAATCAGATA CACCTTCATG TGACCCAGGA GGAGTATGGT 
GCCAGAATTT CCAACCTGCT GATGTATGAT GCTGTGACTT CTGACATCCT GCGCCGCTGG 
TTATATCCCC TTACACCTGA GACTAATTTC ACTGACCAGG ATGCACATAC TTACACTCAC 
TGTCGTCACA ACATCTATCG AGGATCAGAT GTCAGCCGAG GCCATGGAAG CATACTGAGG 
GAGAATGTGC TGATTGGGAC TAATACAACT ATTGGATCTA AATGCAGCGT CAGCAACAGT 
ACCATTGGGA GGAACTGCAG CATCGGAGAC AGAGTAGTTC TAGAGAATGT ACATGTCTGG 
GATAGAGTTC ACATTGAAGA TGATGTTACT ATCAAAAATT CCATAATTTG TAATGATGTT 
GTTGTGAAGA AAAGGGTGCA GATAAATCCT TACTGCGTGT TGACGTCAAA GGTAGTGATT 
GGTCCTGATA TACAACTGCC TGAAAGTACA GTTATTTCTC TTCATCATCC TGAAGAGGAT 
GAAAATGATG ATGATGAGTT TAGTGATGAT GCAGGAGCTG GAAAACAGGC AGAAAGAATG 
AAGGTCAAAG TATACAATAA AAAGGAGGTT GGTTCAGAAG GTCAGGGTTA TCTGTGGAAA 
TTTTCAAATC TTGCAGAAGA GGAGGAAGAT GATGAACTCC AGAGGAGTTT GTGGGGACTT 
ACTCTGAACA CTGAGGAGGT CAGTGACAGC GAGAGTGAGA CTGGCAGTAT GCAGCAGTCA 
GAAAGCCACC CAGCTTCACC TGTCCTTGAT GATATTAAAG TTTTTCAGAT GGAAGTTTTG 
GGCACACTGC AGAGGGGTGT GGAAGAAAAT ATATCTTGTG ATAATTTGGT GCTTGAAATT 
AATTCTCTCA AATATGCCTA TAACATATCT CTCACGGAGG TGATGCAGGT ATTGAGTAAG 
GTTGTCTTGG AGTTTCCACT CCATCAGCTT GATGGAACTT TGGATGTGAA CCGCTACTGC 
AGTACTCTAA TTCCTCTTCT GAAAATATGG ACCCCCCTTT TTAAAAACTA CATAAAACGG 
GCTTCAGATC ATTTGTGTTG CTTGGGAGCC ATAGAAGAAT TTTTTTTGGA CAATGAAACA 
CTATGGGCTG CCATTGCAAA GGTACTCATG GCCTTCTACC AGCAAGATGT ATTAGCAGAA 
GAGATGATTC TACGATGGTT TTCACAGACT GATGTTACAG AGAAGGGTCG GCAATTTAGG 
AAAAAGCAGG TGCTCCAGAA GTTCATACAG TGGTTAGAAG AAGCCGAAGA AGAATCTTCA 
GAAGATGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012818818.1
CDS	168..2039
Translation	MANDIKEHLRNSKWCRPTSPNVVRFVTSELYRSLGDVLRDVDAKSLVRSDFILVTGDVVSNINIEAALEQHRTRRKLEKNVSVMTMILKESSPGHRTRCQEDDVIIAMDSKTKRVLLYQKSQGLTRFHFPVSIFQSNTDEIELRHDLLDCHISICSPQVAELFTDNFDYQTRDDFVRGILVNEEILGNQIHLHVTQEEYGARISNLLMYDAVTSDILRRWLYPLTPETNFTDQDAHTYTHCRHNIYRGSDVSRGHGSILRENVLIGTNTTIGSKCSVSNSTIGRNCSIGDRVVLENVHVWDRVHIEDDVTIKNSIICNDVVVKKRVQINPYCVLTSKVVIGPDIQLPESTVISLHHPEEDENDDDEFSDDAGAGKQAERMKVKVYNKKEVGSEGQGYLWKFSNLAEEEEDDELQRSLWGLTLNTEEVSDSESETGSMQQSESHPASPVLDDIKVFQMEVLGTLQRGVEENISCDNLVLEINSLKYAYNISLTEVMQVLSKVVLEFPLHQLDGTLDVNRYCSTLIPLLKIWTPLFKNYIKRASDHLCCLGAIEEFFLDNETLWAAIAKVLMAFYQQDVLAEEMILRWFSQTDVTEKGRQFRKKQVLQKFIQWLEEAEEESSEDE

Target ORF information:

RefSeq Version	XM_012963364.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis eukaryotic translation initiation factor 2B subunit epsilon (eif2b5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963364.2

ORF Insert Sequence:

ATGGCAAATG ATATTAAGGA GCATCTGAGA AATTCAAAGT GGTGCAGACC TACCAGCCCC 
AATGTGGTAC GGTTTGTGAC CTCTGAGCTT TACAGATCAC TTGGTGATGT TCTGAGAGAT 
GTAGATGCCA AATCTTTAGT AAGATCTGAC TTCATTCTTG TCACTGGTGA TGTGGTGTCC 
AACATCAACA TTGAAGCAGC TTTGGAGCAA CATAGGACAC GGCGGAAGTT AGAAAAAAAT 
GTGTCTGTGA TGACAATGAT CCTTAAGGAG TCCTCTCCAG GTCACCGCAC AAGATGTCAA 
GAGGATGATG TTATAATTGC TATGGACAGT AAGACCAAAC GAGTGCTGCT ATACCAGAAA 
AGTCAGGGCC TGACACGCTT TCACTTTCCA GTGAGCATTT TCCAGAGTAA CACTGATGAA 
ATTGAGCTGC GCCACGATTT ATTGGATTGT CACATCAGCA TTTGCTCTCC ACAAGTTGCA 
GAACTGTTTA CAGATAACTT TGATTACCAG ACTAGGGATG ATTTTGTCCG TGGTATTTTG 
GTTAATGAAG AGATTCTGGG CAATCAGATA CACCTTCATG TGACCCAGGA GGAGTATGGT 
GCCAGAATTT CCAACCTGCT GATGTATGAT GCTGTGACTT CTGACATCCT GCGCCGCTGG 
TTATATCCCC TTACACCTGA GACTAATTTC ACTGACCAGG ATGCACATAC TTACACTCAC 
TGTCGTCACA ACATCTATCG AGGATCAGAT GTCAGCCGAG GCCATGGAAG CATACTGAGG 
GAGAATGTGC TGATTGGGAC TAATACAACT ATTGGATCTA AATGCAGCGT CAGCAACAGT 
ACCATTGGGA GGAACTGCAG CATCGGAGAC AGAGTAGTTC TAGAGAATGT ACATGTCTGG 
GATAGAGTTC ACATTGAAGA TGATGTTACT ATCAAAAATT CCATAATTTG TAATGATGTT 
GTTGTGAAGA AAAGGGTGCA GATAAATCCT TACTGCGTGT TGACGTCAAA GGTAGTGATT 
GGTCCTGATA TACAACTGCC TGAAAGTACA GTTATTTCTC TTCATCATCC TGAAGAGGAT 
GAAAATGATG ATGATGAGTT TAGTGATGAT GCAGGAGCTG GAAAACAGGC AGAAAGAATG 
AAGGTCAAAG TATACAATAA AAAGGAGGTT GGTTCAGAAG GTCAGGGTTA TCTGTGGAAA 
TTTTCAAATC TTGCAGAAGA GGAGGAAGAT GATGAACTCC AGAGGAGTTT GTGGGGACTT 
ACTCTGAACA CTGAGGAGGT CAGTGACAGC GAGAGTGAGA CTGGCAGTAT GCAGCAGTCA 
GAAAGCCACC CAGCTTCACC TGTCCTTGAT GATATTAAAG TTTTTCAGAT GGAAGTTTTG 
GGCACACTGC AGAGGGGTGT GGAAGAAAAT ATATCTTGTG ATAATTTGGT GCTTGAAATT 
AATTCTCTCA AATATGCCTA TAACATATCT CTCACGGAGG TGATGCAGGT ATTGAGTAAG 
GTTGTCTTGG AGTTTCCACT CCATCAGCTT GATGGAACTT TGGATGTGAA CCGCTACTGC 
AGTACTCTAA TTCCTCTTCT GAAAATATGG ACCCCCCTTT TTAAAAACTA CATAAAACGG 
GCTTCAGATC ATTTGTGTTG CTTGGGAGCC ATAGAAGAAT TTTTTTTGGA CAATGAAACA 
CTATGGGCTG CCATTGCAAA GGTACTCATG GCCTTCTACC AGCAAGATGT ATTAGCAGAA 
GAGATGATTC TACGATGGTT TTCACAGACT GATGTTACAG AGAAGGGTCG GCAATTTAGG 
AAAAAGCAGG TGCTCCAGAA GTTCATACAG TGGTTAGAAG AAGCCGAAGA AGAATCTTCA 
GAAGATGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eif2b5 ( XM_002935139.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935185.2 Xenopus tropicalis eukaryotic translation initiation factor 2B subunit epsilon (eif2b5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa10192
Clone ID Related Accession (Same CDS sequence)	XM_002935139.4
Accession Version	XM_002935139.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	translation initiation factor eIF-2B subunit epsilon isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002935139.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAGCG ATGCGGTGGC CGGAGAGGGA CATTTTAACA GCTTCGAGAA TTCGCATGAC 
ATGGCTTCGC GTGCTGTGAA ACGCGGTACT GGGGGAGATG AAGAGCAGCT TCCTCTGCAG 
GCAGTGTTGG TTGCGGACAG TTTCAATAGG AGGTTTTATC CAATCACAAA GGACCGGCCA 
CGGGCTCTTA TCCCACTGGC TAATGTGGCT CTGATAGATT ATACGCTGGA GTTTCTTACA 
GCCACTGGAG TCAAAGAAAC TTTTGTGTTT TGCTGCTGGA TGGCAAATGA TATTAAGGAG 
CATCTGAGAA ATTCAAAGTG GTGCAGACCT ACCAGCCCCA ATGTGGTACG GTTTGTGACC 
TCTGAGCTTT ACAGATCACT TGGTGATGTT CTGAGAGATG TAGATGCCAA ATCTTTAGTA 
AGATCTGACT TCATTCTTGT CACTGGTGAT GTGGTGTCCA ACATCAACAT TGAAGCAGCT 
TTGGAGCAAC ATAGGACACG GCGGAAGTTA GAAAAAAATG TGTCTGTGAT GACAATGATC 
CTTAAGGAGT CCTCTCCAGG TCACCGCACA AGATGTCAAG AGGATGATGT TATAATTGCT 
ATGGACAGTA AGACCAAACG AGTGCTGCTA TACCAGAAAA GTCAGGGCCT GACACGCTTT 
CACTTTCCAG TGAGCATTTT CCAGAGTAAC ACTGATGAAA TTGAGCTGCG CCACGATTTA 
TTGGATTGTC ACATCAGCAT TTGCTCTCCA CAAGTTGCAG AACTGTTTAC AGATAACTTT 
GATTACCAGA CTAGGGATGA TTTTGTCCGT GGTATTTTGG TTAATGAAGA GATTCTGGGC 
AATCAGATAC ACCTTCATGT GACCCAGGAG GAGTATGGTG CCAGAATTTC CAACCTGCTG 
ATGTATGATG CTGTGACTTC TGACATCCTG CGCCGCTGGT TATATCCCCT TACACCTGAG 
ACTAATTTCA CTGACCAGGA TGCACATACT TACACTCACT GTCGTCACAA CATCTATCGA 
GGATCAGATG TCAGCCGAGG CCATGGAAGC ATACTGAGGG AGAATGTGCT GATTGGGACT 
AATACAACTA TTGGATCTAA ATGCAGCGTC AGCAACAGTA CCATTGGGAG GAACTGCAGC 
ATCGGAGACA GAGTAGTTCT AGAGAATGTA CATGTCTGGG ATAGAGTTCA CATTGAAGAT 
GATGTTACTA TCAAAAATTC CATAATTTGT AATGATGTTG TTGTGAAGAA AAGGGTGCAG 
ATAAATCCTT ACTGCGTGTT GACGTCAAAG GTAGTGATTG GTCCTGATAT ACAACTGCCT 
GAAAGTACAG TTATTTCTCT TCATCATCCT GAAGAGGATG AAAATGATGA TGATGAGTTT 
AGTGATGATG CAGGAGCTGG AAAACAGGCA GAAAGAATGA AGGTCAAAGT ATACAATAAA 
AAGGAGGTTG GTTCAGAAGG TCAGGGTTAT CTGTGGAAAT TTTCAAATCT TGCAGAAGAG 
GAGGAAGATG ATGAACTCCA GAGGAGTTTG TGGGGACTTA CTCTGAACAC TGAGGAGGTC 
AGTGACAGCG AGAGTGAGAC TGGCAGTATG CAGCAGTCAG AAAGCCACCC AGCTTCACCT 
GTCCTTGATG ATATTAAAGT TTTTCAGATG GAAGTTTTGG GCACACTGCA GAGGGGTGTG 
GAAGAAAATA TATCTTGTGA TAATTTGGTG CTTGAAATTA ATTCTCTCAA ATATGCCTAT 
AACATATCTC TCACGGAGGT GATGCAGGTA TTGAGTAAGG TTGTCTTGGA GTTTCCACTC 
CATCAGCTTG ATGGAACTTT GGATGTGAAC CGCTACTGCA GTACTCTAAT TCCTCTTCTG 
AAAATATGGA CCCCCCTTTT TAAAAACTAC ATAAAACGGG CTTCAGATCA TTTGTGTTGC 
TTGGGAGCCA TAGAAGAATT TTTTTTGGAC AATGAAACAC TATGGGCTGC CATTGCAAAG 
GTACTCATGG CCTTCTACCA GCAAGATGTA TTAGCAGAAG AGATGATTCT ACGATGGTTT 
TCACAGACTG ATGTTACAGA GAAGGGTCGG CAATTTAGGA AAAAGCAGGT GCTCCAGAAG 
TTCATACAGT GGTTAGAAGA AGCCGAAGAA GAATCTTCAG AAGATGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935185.2
CDS	29..2179
Translation	MQSDAVAGEGHFNSFENSHDMASRAVKRGTGGDEEQLPLQAVLVADSFNRRFYPITKDRPRALIPLANVALIDYTLEFLTATGVKETFVFCCWMANDIKEHLRNSKWCRPTSPNVVRFVTSELYRSLGDVLRDVDAKSLVRSDFILVTGDVVSNINIEAALEQHRTRRKLEKNVSVMTMILKESSPGHRTRCQEDDVIIAMDSKTKRVLLYQKSQGLTRFHFPVSIFQSNTDEIELRHDLLDCHISICSPQVAELFTDNFDYQTRDDFVRGILVNEEILGNQIHLHVTQEEYGARISNLLMYDAVTSDILRRWLYPLTPETNFTDQDAHTYTHCRHNIYRGSDVSRGHGSILRENVLIGTNTTIGSKCSVSNSTIGRNCSIGDRVVLENVHVWDRVHIEDDVTIKNSIICNDVVVKKRVQINPYCVLTSKVVIGPDIQLPESTVISLHHPEEDENDDDEFSDDAGAGKQAERMKVKVYNKKEVGSEGQGYLWKFSNLAEEEEDDELQRSLWGLTLNTEEVSDSESETGSMQQSESHPASPVLDDIKVFQMEVLGTLQRGVEENISCDNLVLEINSLKYAYNISLTEVMQVLSKVVLEFPLHQLDGTLDVNRYCSTLIPLLKIWTPLFKNYIKRASDHLCCLGAIEEFFLDNETLWAAIAKVLMAFYQQDVLAEEMILRWFSQTDVTEKGRQFRKKQVLQKFIQWLEEAEEESSEDE

Target ORF information:

RefSeq Version	XM_002935139.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis eukaryotic translation initiation factor 2B subunit epsilon (eif2b5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935139.4

ORF Insert Sequence:

ATGCAAAGCG ATGCGGTGGC CGGAGAGGGA CATTTTAACA GCTTCGAGAA TTCGCATGAC 
ATGGCTTCGC GTGCTGTGAA ACGCGGTACT GGGGGAGATG AAGAGCAGCT TCCTCTGCAG 
GCAGTGTTGG TTGCGGACAG TTTCAATAGG AGGTTTTATC CAATCACAAA GGACCGGCCA 
CGGGCTCTTA TCCCACTGGC TAATGTGGCT CTGATAGATT ATACGCTGGA GTTTCTTACA 
GCCACTGGAG TCAAAGAAAC TTTTGTGTTT TGCTGCTGGA TGGCAAATGA TATTAAGGAG 
CATCTGAGAA ATTCAAAGTG GTGCAGACCT ACCAGCCCCA ATGTGGTACG GTTTGTGACC 
TCTGAGCTTT ACAGATCACT TGGTGATGTT CTGAGAGATG TAGATGCCAA ATCTTTAGTA 
AGATCTGACT TCATTCTTGT CACTGGTGAT GTGGTGTCCA ACATCAACAT TGAAGCAGCT 
TTGGAGCAAC ATAGGACACG GCGGAAGTTA GAAAAAAATG TGTCTGTGAT GACAATGATC 
CTTAAGGAGT CCTCTCCAGG TCACCGCACA AGATGTCAAG AGGATGATGT TATAATTGCT 
ATGGACAGTA AGACCAAACG AGTGCTGCTA TACCAGAAAA GTCAGGGCCT GACACGCTTT 
CACTTTCCAG TGAGCATTTT CCAGAGTAAC ACTGATGAAA TTGAGCTGCG CCACGATTTA 
TTGGATTGTC ACATCAGCAT TTGCTCTCCA CAAGTTGCAG AACTGTTTAC AGATAACTTT 
GATTACCAGA CTAGGGATGA TTTTGTCCGT GGTATTTTGG TTAATGAAGA GATTCTGGGC 
AATCAGATAC ACCTTCATGT GACCCAGGAG GAGTATGGTG CCAGAATTTC CAACCTGCTG 
ATGTATGATG CTGTGACTTC TGACATCCTG CGCCGCTGGT TATATCCCCT TACACCTGAG 
ACTAATTTCA CTGACCAGGA TGCACATACT TACACTCACT GTCGTCACAA CATCTATCGA 
GGATCAGATG TCAGCCGAGG CCATGGAAGC ATACTGAGGG AGAATGTGCT GATTGGGACT 
AATACAACTA TTGGATCTAA ATGCAGCGTC AGCAACAGTA CCATTGGGAG GAACTGCAGC 
ATCGGAGACA GAGTAGTTCT AGAGAATGTA CATGTCTGGG ATAGAGTTCA CATTGAAGAT 
GATGTTACTA TCAAAAATTC CATAATTTGT AATGATGTTG TTGTGAAGAA AAGGGTGCAG 
ATAAATCCTT ACTGCGTGTT GACGTCAAAG GTAGTGATTG GTCCTGATAT ACAACTGCCT 
GAAAGTACAG TTATTTCTCT TCATCATCCT GAAGAGGATG AAAATGATGA TGATGAGTTT 
AGTGATGATG CAGGAGCTGG AAAACAGGCA GAAAGAATGA AGGTCAAAGT ATACAATAAA 
AAGGAGGTTG GTTCAGAAGG TCAGGGTTAT CTGTGGAAAT TTTCAAATCT TGCAGAAGAG 
GAGGAAGATG ATGAACTCCA GAGGAGTTTG TGGGGACTTA CTCTGAACAC TGAGGAGGTC 
AGTGACAGCG AGAGTGAGAC TGGCAGTATG CAGCAGTCAG AAAGCCACCC AGCTTCACCT 
GTCCTTGATG ATATTAAAGT TTTTCAGATG GAAGTTTTGG GCACACTGCA GAGGGGTGTG 
GAAGAAAATA TATCTTGTGA TAATTTGGTG CTTGAAATTA ATTCTCTCAA ATATGCCTAT 
AACATATCTC TCACGGAGGT GATGCAGGTA TTGAGTAAGG TTGTCTTGGA GTTTCCACTC 
CATCAGCTTG ATGGAACTTT GGATGTGAAC CGCTACTGCA GTACTCTAAT TCCTCTTCTG 
AAAATATGGA CCCCCCTTTT TAAAAACTAC ATAAAACGGG CTTCAGATCA TTTGTGTTGC 
TTGGGAGCCA TAGAAGAATT TTTTTTGGAC AATGAAACAC TATGGGCTGC CATTGCAAAG 
GTACTCATGG CCTTCTACCA GCAAGATGTA TTAGCAGAAG AGATGATTCT ACGATGGTTT 
TCACAGACTG ATGTTACAGA GAAGGGTCGG CAATTTAGGA AAAAGCAGGT GCTCCAGAAG 
TTCATACAGT GGTTAGAAGA AGCCGAAGAA GAATCTTCAG AAGATGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eif2b5 ( XM_012963364.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818818.1 Xenopus tropicalis eukaryotic translation initiation factor 2B subunit epsilon (eif2b5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa33981
Clone ID Related Accession (Same CDS sequence)	XM_012963364.3 , XM_012963364.2
Accession Version	XM_012963364.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	translation initiation factor eIF-2B subunit epsilon isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012963364.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG ATATTAAGGA GCATCTGAGA AATTCAAAGT GGTGCAGACC TACCAGCCCC 
AATGTGGTAC GGTTTGTGAC CTCTGAGCTT TACAGATCAC TTGGTGATGT TCTGAGAGAT 
GTAGATGCCA AATCTTTAGT AAGATCTGAC TTCATTCTTG TCACTGGTGA TGTGGTGTCC 
AACATCAACA TTGAAGCAGC TTTGGAGCAA CATAGGACAC GGCGGAAGTT AGAAAAAAAT 
GTGTCTGTGA TGACAATGAT CCTTAAGGAG TCCTCTCCAG GTCACCGCAC AAGATGTCAA 
GAGGATGATG TTATAATTGC TATGGACAGT AAGACCAAAC GAGTGCTGCT ATACCAGAAA 
AGTCAGGGCC TGACACGCTT TCACTTTCCA GTGAGCATTT TCCAGAGTAA CACTGATGAA 
ATTGAGCTGC GCCACGATTT ATTGGATTGT CACATCAGCA TTTGCTCTCC ACAAGTTGCA 
GAACTGTTTA CAGATAACTT TGATTACCAG ACTAGGGATG ATTTTGTCCG TGGTATTTTG 
GTTAATGAAG AGATTCTGGG CAATCAGATA CACCTTCATG TGACCCAGGA GGAGTATGGT 
GCCAGAATTT CCAACCTGCT GATGTATGAT GCTGTGACTT CTGACATCCT GCGCCGCTGG 
TTATATCCCC TTACACCTGA GACTAATTTC ACTGACCAGG ATGCACATAC TTACACTCAC 
TGTCGTCACA ACATCTATCG AGGATCAGAT GTCAGCCGAG GCCATGGAAG CATACTGAGG 
GAGAATGTGC TGATTGGGAC TAATACAACT ATTGGATCTA AATGCAGCGT CAGCAACAGT 
ACCATTGGGA GGAACTGCAG CATCGGAGAC AGAGTAGTTC TAGAGAATGT ACATGTCTGG 
GATAGAGTTC ACATTGAAGA TGATGTTACT ATCAAAAATT CCATAATTTG TAATGATGTT 
GTTGTGAAGA AAAGGGTGCA GATAAATCCT TACTGCGTGT TGACGTCAAA GGTAGTGATT 
GGTCCTGATA TACAACTGCC TGAAAGTACA GTTATTTCTC TTCATCATCC TGAAGAGGAT 
GAAAATGATG ATGATGAGTT TAGTGATGAT GCAGGAGCTG GAAAACAGGC AGAAAGAATG 
AAGGTCAAAG TATACAATAA AAAGGAGGTT GGTTCAGAAG GTCAGGGTTA TCTGTGGAAA 
TTTTCAAATC TTGCAGAAGA GGAGGAAGAT GATGAACTCC AGAGGAGTTT GTGGGGACTT 
ACTCTGAACA CTGAGGAGGT CAGTGACAGC GAGAGTGAGA CTGGCAGTAT GCAGCAGTCA 
GAAAGCCACC CAGCTTCACC TGTCCTTGAT GATATTAAAG TTTTTCAGAT GGAAGTTTTG 
GGCACACTGC AGAGGGGTGT GGAAGAAAAT ATATCTTGTG ATAATTTGGT GCTTGAAATT 
AATTCTCTCA AATATGCCTA TAACATATCT CTCACGGAGG TGATGCAGGT ATTGAGTAAG 
GTTGTCTTGG AGTTTCCACT CCATCAGCTT GATGGAACTT TGGATGTGAA CCGCTACTGC 
AGTACTCTAA TTCCTCTTCT GAAAATATGG ACCCCCCTTT TTAAAAACTA CATAAAACGG 
GCTTCAGATC ATTTGTGTTG CTTGGGAGCC ATAGAAGAAT TTTTTTTGGA CAATGAAACA 
CTATGGGCTG CCATTGCAAA GGTACTCATG GCCTTCTACC AGCAAGATGT ATTAGCAGAA 
GAGATGATTC TACGATGGTT TTCACAGACT GATGTTACAG AGAAGGGTCG GCAATTTAGG 
AAAAAGCAGG TGCTCCAGAA GTTCATACAG TGGTTAGAAG AAGCCGAAGA AGAATCTTCA 
GAAGATGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012818818.1
CDS	351..2222
Translation	MANDIKEHLRNSKWCRPTSPNVVRFVTSELYRSLGDVLRDVDAKSLVRSDFILVTGDVVSNINIEAALEQHRTRRKLEKNVSVMTMILKESSPGHRTRCQEDDVIIAMDSKTKRVLLYQKSQGLTRFHFPVSIFQSNTDEIELRHDLLDCHISICSPQVAELFTDNFDYQTRDDFVRGILVNEEILGNQIHLHVTQEEYGARISNLLMYDAVTSDILRRWLYPLTPETNFTDQDAHTYTHCRHNIYRGSDVSRGHGSILRENVLIGTNTTIGSKCSVSNSTIGRNCSIGDRVVLENVHVWDRVHIEDDVTIKNSIICNDVVVKKRVQINPYCVLTSKVVIGPDIQLPESTVISLHHPEEDENDDDEFSDDAGAGKQAERMKVKVYNKKEVGSEGQGYLWKFSNLAEEEEDDELQRSLWGLTLNTEEVSDSESETGSMQQSESHPASPVLDDIKVFQMEVLGTLQRGVEENISCDNLVLEINSLKYAYNISLTEVMQVLSKVVLEFPLHQLDGTLDVNRYCSTLIPLLKIWTPLFKNYIKRASDHLCCLGAIEEFFLDNETLWAAIAKVLMAFYQQDVLAEEMILRWFSQTDVTEKGRQFRKKQVLQKFIQWLEEAEEESSEDE

Target ORF information:

RefSeq Version	XM_012963364.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis eukaryotic translation initiation factor 2B subunit epsilon (eif2b5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963364.3

ORF Insert Sequence:

ATGGCAAATG ATATTAAGGA GCATCTGAGA AATTCAAAGT GGTGCAGACC TACCAGCCCC 
AATGTGGTAC GGTTTGTGAC CTCTGAGCTT TACAGATCAC TTGGTGATGT TCTGAGAGAT 
GTAGATGCCA AATCTTTAGT AAGATCTGAC TTCATTCTTG TCACTGGTGA TGTGGTGTCC 
AACATCAACA TTGAAGCAGC TTTGGAGCAA CATAGGACAC GGCGGAAGTT AGAAAAAAAT 
GTGTCTGTGA TGACAATGAT CCTTAAGGAG TCCTCTCCAG GTCACCGCAC AAGATGTCAA 
GAGGATGATG TTATAATTGC TATGGACAGT AAGACCAAAC GAGTGCTGCT ATACCAGAAA 
AGTCAGGGCC TGACACGCTT TCACTTTCCA GTGAGCATTT TCCAGAGTAA CACTGATGAA 
ATTGAGCTGC GCCACGATTT ATTGGATTGT CACATCAGCA TTTGCTCTCC ACAAGTTGCA 
GAACTGTTTA CAGATAACTT TGATTACCAG ACTAGGGATG ATTTTGTCCG TGGTATTTTG 
GTTAATGAAG AGATTCTGGG CAATCAGATA CACCTTCATG TGACCCAGGA GGAGTATGGT 
GCCAGAATTT CCAACCTGCT GATGTATGAT GCTGTGACTT CTGACATCCT GCGCCGCTGG 
TTATATCCCC TTACACCTGA GACTAATTTC ACTGACCAGG ATGCACATAC TTACACTCAC 
TGTCGTCACA ACATCTATCG AGGATCAGAT GTCAGCCGAG GCCATGGAAG CATACTGAGG 
GAGAATGTGC TGATTGGGAC TAATACAACT ATTGGATCTA AATGCAGCGT CAGCAACAGT 
ACCATTGGGA GGAACTGCAG CATCGGAGAC AGAGTAGTTC TAGAGAATGT ACATGTCTGG 
GATAGAGTTC ACATTGAAGA TGATGTTACT ATCAAAAATT CCATAATTTG TAATGATGTT 
GTTGTGAAGA AAAGGGTGCA GATAAATCCT TACTGCGTGT TGACGTCAAA GGTAGTGATT 
GGTCCTGATA TACAACTGCC TGAAAGTACA GTTATTTCTC TTCATCATCC TGAAGAGGAT 
GAAAATGATG ATGATGAGTT TAGTGATGAT GCAGGAGCTG GAAAACAGGC AGAAAGAATG 
AAGGTCAAAG TATACAATAA AAAGGAGGTT GGTTCAGAAG GTCAGGGTTA TCTGTGGAAA 
TTTTCAAATC TTGCAGAAGA GGAGGAAGAT GATGAACTCC AGAGGAGTTT GTGGGGACTT 
ACTCTGAACA CTGAGGAGGT CAGTGACAGC GAGAGTGAGA CTGGCAGTAT GCAGCAGTCA 
GAAAGCCACC CAGCTTCACC TGTCCTTGAT GATATTAAAG TTTTTCAGAT GGAAGTTTTG 
GGCACACTGC AGAGGGGTGT GGAAGAAAAT ATATCTTGTG ATAATTTGGT GCTTGAAATT 
AATTCTCTCA AATATGCCTA TAACATATCT CTCACGGAGG TGATGCAGGT ATTGAGTAAG 
GTTGTCTTGG AGTTTCCACT CCATCAGCTT GATGGAACTT TGGATGTGAA CCGCTACTGC 
AGTACTCTAA TTCCTCTTCT GAAAATATGG ACCCCCCTTT TTAAAAACTA CATAAAACGG 
GCTTCAGATC ATTTGTGTTG CTTGGGAGCC ATAGAAGAAT TTTTTTTGGA CAATGAAACA 
CTATGGGCTG CCATTGCAAA GGTACTCATG GCCTTCTACC AGCAAGATGT ATTAGCAGAA 
GAGATGATTC TACGATGGTT TTCACAGACT GATGTTACAG AGAAGGGTCG GCAATTTAGG 
AAAAAGCAGG TGCTCCAGAA GTTCATACAG TGGTTAGAAG AAGCCGAAGA AGAATCTTCA 
GAAGATGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eif2b5 ( XM_002935139.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935185.3 Xenopus tropicalis eukaryotic translation initiation factor 2B subunit epsilon (eif2b5), transcript variant X1, mRNA.

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CloneID	OXa58481
Clone ID Related Accession (Same CDS sequence)	XM_002935139.5
Accession Version	XM_002935139.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	translation initiation factor eIF-2B subunit epsilon isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002935139.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAGCG ATGCGGTGGC CGGAAAGGGA CATTTTAACA GCTTCGAGAA TTCGCATGAC 
ATGGCTTCGC GTGCTGTGAA ACGCGGTACT GGGGGAGATG AAGAGCAGCT TCCTCTGCAG 
GCAGTGTTGG TTGCGGACAG TTTCAATAGG AGGTTTTATC CAATCACAAA GGACCGGCCA 
CGGGCTCTTA TCCCACTGGC TAATGTGGCT CTGATAGATT ATACGCTGGA GTTTCTTACA 
GCCACTGGAG TCAAAGAAAC TTTTGTGTTT TGCTGCTGGA TGGCAAATGA TATTAAGGAG 
CATCTGAGAA ATTCAAAGTG GTGCAGACCT ACCAGCCCCA ATGTGGTACG GTTTGTGACC 
TCTGAGCTTT ACAGATCACT TGGTGATGTT CTGAGAGATG TAGATGCCAA ATCTTTAGTA 
AGATCTGACT TCATTCTTGT CACTGGTGAT GTGGTGTCCA ACATCAACAT TGAAGCAGCT 
TTGGAGCAAC ATAGGACACG GCGGAAGTTA GAAAAAAATG TGTCTGTGAT GACAATGATC 
CTTAAGGAGT CCTCTCCAGG TCACCGCACA AGATGTCAAG AGGATGATGT TATAATTGCT 
ATGGACAGTA AGACCAAACG AGTGCTGCTA TACCAGAAAA GTCAGGGCCT GACACGCTTT 
CACTTTCCAG TGAGCATTTT CCAGAGTAAC ACTGATGAAA TTGAGCTGCG CCACGATTTA 
TTGGATTGTC ACATCAGCAT TTGCTCTCCA CAAGTTGCAG AACTGTTTAC AGATAACTTT 
GATTACCAGA CTAGGGATGA TTTTGTCCGT GGTATTTTGG TTAATGAAGA GATTCTGGGC 
AATCAGATAC ACCTTCATGT GACCCAGGAG GAGTATGGTG CCAGAATTTC CAACCTGCTG 
ATGTATGATG CTGTGACTTC TGACATCCTG CGCCGCTGGT TATATCCCCT TACACCTGAG 
ACTAATTTCA CTGACCAGGA TGCACATACT TACACTCACT GTCGTCACAA CATCTATCGA 
GGATCAGATG TCAGCCGAGG CCATGGAAGC ATACTGAGGG AGAATGTGCT GATTGGGACT 
AATACAACTA TTGGATCTAA ATGCAGCGTC AGCAACAGTA CCATTGGGAG GAACTGCAGC 
ATCGGAGACA GAGTAGTTCT AGAGAATGTA CATGTCTGGG ATAGAGTTCA CATTGAAGAT 
GATGTTACTA TCAAAAATTC CATAATTTGT AATGATGTTG TTGTGAAGAA AAGGGTGCAG 
ATAAATCCTT ACTGCGTGTT GACGTCAAAG GTAGTGATTG GTCCTGATAT ACAACTGCCT 
GAAAGTACAG TTATTTCTCT TCATCATCCT GAAGAGGATG AAAATGATGA TGATGAGTTT 
AGTGATGATG CAGGAGCTGG AAAACAGGCA GAAAGAATGA AGGTCAAAGT ATACAATAAA 
AAGGAGGTTG GTTCAGAAGG TCAGGGTTAT CTGTGGAAAT TTTCAAATCT TGCAGAAGAG 
GAGGAAGATG ATGAACTCCA GAGGAGTTTG TGGGGACTTA CTCTGAACAC TGAGGAGGTC 
AGTGACAGCG AGAGTGAGAC TGGCAGTATG CAGCAGTCAG AAAGCCACCC AGCTTCACCT 
GTCCTTGATG ATATTAAAGT TTTTCAGATG GAAGTTTTGG GCACACTGCA GAGGGGTGTG 
GAAGAAAATA TATCTTGTGA TAATTTGGTG CTTGAAATTA ATTCTCTCAA ATATGCCTAT 
AACATATCTC TCACGGAGGT GATGCAGGTA TTGAGTAAGG TTGTCTTGGA GTTTCCACTC 
CATCAGCTTG ATGGAACTTT GGATGTGAAC CGCTACTGCA GTACTCTAAT TCCTCTTCTG 
AAAATATGGA CCCCCCTTTT TAAAAACTAC ATAAAACGGG CTTCAGATCA TTTGTGTTGC 
TTGGGAGCCA TAGAAGAATT TTTTTTGGAC AATGAAACAC TATGGGCTGC CATTGCAAAG 
GTACTCATGG CCTTCTACCA GCAAGATGTA TTAGCAGAAG AGATGATTCT ACGATGGTTT 
TCACAGACTG ATGTTACAGA GAAGGGTCGG CAATTTAGGA AAAAGCAGGT GCTCCAGAAG 
TTCATACAGT GGTTAGAAGA AGCCGAAGAA GAATCTTCAG AAGATGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935185.3
CDS	30..2180
Translation	MQSDAVAGKGHFNSFENSHDMASRAVKRGTGGDEEQLPLQAVLVADSFNRRFYPITKDRPRALIPLANVALIDYTLEFLTATGVKETFVFCCWMANDIKEHLRNSKWCRPTSPNVVRFVTSELYRSLGDVLRDVDAKSLVRSDFILVTGDVVSNINIEAALEQHRTRRKLEKNVSVMTMILKESSPGHRTRCQEDDVIIAMDSKTKRVLLYQKSQGLTRFHFPVSIFQSNTDEIELRHDLLDCHISICSPQVAELFTDNFDYQTRDDFVRGILVNEEILGNQIHLHVTQEEYGARISNLLMYDAVTSDILRRWLYPLTPETNFTDQDAHTYTHCRHNIYRGSDVSRGHGSILRENVLIGTNTTIGSKCSVSNSTIGRNCSIGDRVVLENVHVWDRVHIEDDVTIKNSIICNDVVVKKRVQINPYCVLTSKVVIGPDIQLPESTVISLHHPEEDENDDDEFSDDAGAGKQAERMKVKVYNKKEVGSEGQGYLWKFSNLAEEEEDDELQRSLWGLTLNTEEVSDSESETGSMQQSESHPASPVLDDIKVFQMEVLGTLQRGVEENISCDNLVLEINSLKYAYNISLTEVMQVLSKVVLEFPLHQLDGTLDVNRYCSTLIPLLKIWTPLFKNYIKRASDHLCCLGAIEEFFLDNETLWAAIAKVLMAFYQQDVLAEEMILRWFSQTDVTEKGRQFRKKQVLQKFIQWLEEAEEESSEDE

Target ORF information:

RefSeq Version	XM_002935139.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis eukaryotic translation initiation factor 2B subunit epsilon (eif2b5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935139.5

ORF Insert Sequence:

ATGCAAAGCG ATGCGGTGGC CGGAAAGGGA CATTTTAACA GCTTCGAGAA TTCGCATGAC 
ATGGCTTCGC GTGCTGTGAA ACGCGGTACT GGGGGAGATG AAGAGCAGCT TCCTCTGCAG 
GCAGTGTTGG TTGCGGACAG TTTCAATAGG AGGTTTTATC CAATCACAAA GGACCGGCCA 
CGGGCTCTTA TCCCACTGGC TAATGTGGCT CTGATAGATT ATACGCTGGA GTTTCTTACA 
GCCACTGGAG TCAAAGAAAC TTTTGTGTTT TGCTGCTGGA TGGCAAATGA TATTAAGGAG 
CATCTGAGAA ATTCAAAGTG GTGCAGACCT ACCAGCCCCA ATGTGGTACG GTTTGTGACC 
TCTGAGCTTT ACAGATCACT TGGTGATGTT CTGAGAGATG TAGATGCCAA ATCTTTAGTA 
AGATCTGACT TCATTCTTGT CACTGGTGAT GTGGTGTCCA ACATCAACAT TGAAGCAGCT 
TTGGAGCAAC ATAGGACACG GCGGAAGTTA GAAAAAAATG TGTCTGTGAT GACAATGATC 
CTTAAGGAGT CCTCTCCAGG TCACCGCACA AGATGTCAAG AGGATGATGT TATAATTGCT 
ATGGACAGTA AGACCAAACG AGTGCTGCTA TACCAGAAAA GTCAGGGCCT GACACGCTTT 
CACTTTCCAG TGAGCATTTT CCAGAGTAAC ACTGATGAAA TTGAGCTGCG CCACGATTTA 
TTGGATTGTC ACATCAGCAT TTGCTCTCCA CAAGTTGCAG AACTGTTTAC AGATAACTTT 
GATTACCAGA CTAGGGATGA TTTTGTCCGT GGTATTTTGG TTAATGAAGA GATTCTGGGC 
AATCAGATAC ACCTTCATGT GACCCAGGAG GAGTATGGTG CCAGAATTTC CAACCTGCTG 
ATGTATGATG CTGTGACTTC TGACATCCTG CGCCGCTGGT TATATCCCCT TACACCTGAG 
ACTAATTTCA CTGACCAGGA TGCACATACT TACACTCACT GTCGTCACAA CATCTATCGA 
GGATCAGATG TCAGCCGAGG CCATGGAAGC ATACTGAGGG AGAATGTGCT GATTGGGACT 
AATACAACTA TTGGATCTAA ATGCAGCGTC AGCAACAGTA CCATTGGGAG GAACTGCAGC 
ATCGGAGACA GAGTAGTTCT AGAGAATGTA CATGTCTGGG ATAGAGTTCA CATTGAAGAT 
GATGTTACTA TCAAAAATTC CATAATTTGT AATGATGTTG TTGTGAAGAA AAGGGTGCAG 
ATAAATCCTT ACTGCGTGTT GACGTCAAAG GTAGTGATTG GTCCTGATAT ACAACTGCCT 
GAAAGTACAG TTATTTCTCT TCATCATCCT GAAGAGGATG AAAATGATGA TGATGAGTTT 
AGTGATGATG CAGGAGCTGG AAAACAGGCA GAAAGAATGA AGGTCAAAGT ATACAATAAA 
AAGGAGGTTG GTTCAGAAGG TCAGGGTTAT CTGTGGAAAT TTTCAAATCT TGCAGAAGAG 
GAGGAAGATG ATGAACTCCA GAGGAGTTTG TGGGGACTTA CTCTGAACAC TGAGGAGGTC 
AGTGACAGCG AGAGTGAGAC TGGCAGTATG CAGCAGTCAG AAAGCCACCC AGCTTCACCT 
GTCCTTGATG ATATTAAAGT TTTTCAGATG GAAGTTTTGG GCACACTGCA GAGGGGTGTG 
GAAGAAAATA TATCTTGTGA TAATTTGGTG CTTGAAATTA ATTCTCTCAA ATATGCCTAT 
AACATATCTC TCACGGAGGT GATGCAGGTA TTGAGTAAGG TTGTCTTGGA GTTTCCACTC 
CATCAGCTTG ATGGAACTTT GGATGTGAAC CGCTACTGCA GTACTCTAAT TCCTCTTCTG 
AAAATATGGA CCCCCCTTTT TAAAAACTAC ATAAAACGGG CTTCAGATCA TTTGTGTTGC 
TTGGGAGCCA TAGAAGAATT TTTTTTGGAC AATGAAACAC TATGGGCTGC CATTGCAAAG 
GTACTCATGG CCTTCTACCA GCAAGATGTA TTAGCAGAAG AGATGATTCT ACGATGGTTT 
TCACAGACTG ATGTTACAGA GAAGGGTCGG CAATTTAGGA AAAAGCAGGT GCTCCAGAAG 
TTCATACAGT GGTTAGAAGA AGCCGAAGAA GAATCTTCAG AAGATGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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eif2b5 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム