trpm6 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	trpm6
Entrez Gene ID	100487366
Full Name	transient receptor potential cation channel, subfamily M, member 6
Synonyms	XTRPM6
General protein information	Preferred Names transient receptor potential cation channel, subfamily M, member 6 Names transient receptor potential cation channel subfamily M member 6
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002940033.4	XP_002940079.2	transient receptor potential cation channel subfamily M member 6 isoform X1
XM_012971667.2	XP_012827121.1	transient receptor potential cation channel subfamily M member 6 isoform X2
XM_018089406.1	XP_017944895.1	transient receptor potential cation channel subfamily M member 6 isoform X3
XM_012971667.3	XP_012827121.1	transient receptor potential cation channel subfamily M member 6 isoform X5
XM_031890874.1	XP_031746734.1	transient receptor potential cation channel subfamily M member 6 isoform X1
XM_031890880.1	XP_031746740.1	transient receptor potential cation channel subfamily M member 6 isoform X3
XM_031890885.1	XP_031746745.1	transient receptor potential cation channel subfamily M member 6 isoform X4
XM_002940033.5	XP_002940079.2	transient receptor potential cation channel subfamily M member 6 isoform X2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	TRPM6	XP_005210098.1
Rattus norvegicus (Norway rat)	Trpm6	XP_006231205.1
Canis lupus familiaris (dog)	TRPM6	XP_005615911.1
Gallus gallus (chicken)	TRPM6	XP_003643069.2
Xenopus tropicalis (tropical clawed frog)	trpm6	XP_002940079.2
Homo sapiens (human)	TRPM6	NP_001170781.1
Mus musculus (house mouse)	Trpm6	NP_700466.1
Pan troglodytes (chimpanzee)	TRPM6	XP_003312159.1
	TRPM6	XP_002800188.1

Gene Ontology

The following trpm6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the trpm6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa33854	XM_012971667.3 Latest version!	Xenopus tropicalis transient receptor potential cation channel, subfamily M, member 6 (trpm6), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OXa42109	XM_018089406.1 Latest version!	Xenopus tropicalis transient receptor potential cation channel, subfamily M, member 6 (trpm6), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OXa48739	XM_031890880.1 Latest version!	Xenopus tropicalis transient receptor potential cation channel, subfamily M, member 6 (trpm6), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa48740	XM_031890885.1 Latest version!	Xenopus tropicalis transient receptor potential cation channel, subfamily M, member 6 (trpm6), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa05105	XM_002940033.5 Latest version!	Xenopus tropicalis transient receptor potential cation channel, subfamily M, member 6 (trpm6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa48738	XM_031890874.1 Latest version!	Xenopus tropicalis transient receptor potential cation channel, subfamily M, member 6 (trpm6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OXa33854	XM_012971667.2	Xenopus tropicalis transient receptor potential cation channel, subfamily M, member 6 (trpm6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OXa05105	XM_002940033.4	Xenopus tropicalis transient receptor potential cation channel, subfamily M, member 6 (trpm6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

trpm6 ( XM_012971667.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012827121.1 Xenopus tropicalis transient receptor potential cation channel, subfamily M, member 6 (trpm6), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa33854
Clone ID Related Accession (Same CDS sequence)	XM_012971667.2 , XM_012971667.3
Accession Version	XM_012971667.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transient receptor potential cation channel subfamily M member 6 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012971667.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTCC AGCTAAAGTC TCAGAAATCA TGGATTGAAG GGCTCTTCAA TAAAAGAGAA 
TGTGCAAAAA TCATACCGTC ATCAAAAGAG CCACACAGAT GCCACGGTGG ATGTCAGGTT 
TGCCAGAATT TAATAAGGTG TTGTTGTGGA CGGTTAATTG GAGACCACCC TGGGTTAGAT 
TATGGATGGC CAATTAATCC TTCTCCCCAA GAAAGAGAAG ACGAAGAGTG GAGTATTCTA 
AATCATACAA AACCAAGTCC CACTGATGCA TTTGGCACAA TAAATTTTCA AGATGGAGAT 
CACACCTATC ATGCAAAGTA CATAAGAATA TCCTATGATA CCACATTGGA ACTGTTAATG 
CATCTGATGA TTAAAGAATG GCAAATGGAA CTGCCCAAGC TTGTGATCTC TGTCCATGGG 
GGCATACAGA ATTTTAAACT TCCGTCCAAA ATAAAGCAAG TTTTTGGCAA AGGATTGGTG 
AAAGCTGCTG AGACAACAGG AGCATGGATC CTTACAGAGG GCATTAATAC TGGGGCCTCC 
AAACATGTGG GTGATGCTTT AAAAGCACAC GCTTCTCAAC ACCTGCGAAA AATCTGTGCA 
GTTGGCATAC CACCATGGGG AGCTATTGAG AATCAGCAGG ATCTCATTGG CAAAGACGTT 
GTGTGCCTGT ATCAGACACT GATAAATCCA ATGAGCAAAC TGACAAGTCT CAATAGCATG 
CACTCCCATT TTATAATGGT GGATGATGGC ACAGTTGGAA AGTATGGTAA TGAAATGAAA 
TTAAGGAGAA ACTTGGAAGA CTTCATTTCC CTTCAGAAGA TCCATACCCG GATGGGGCAA 
GGAGTGCCTG TGGTAGGGCT TGTTATAGAA GGTGGTCCAA ATGTCATCTT GATGGTATGG 
GAATATGTGA GGAGCACTCC ACCAGTACCA GTTGTAGTGT GTGAAGGAAC AGGCAGAGCA 
GCTGATATAT TGGCATTTAC TCACAAGCAC ACAACGGACA CAGAGCAGAT TAGTCCACAG 
CTGAAAGAAG AAATCTTAGA GATGATTCAG AAGACATTTA ATCTTGGCCA CAGACAATCA 
AACCATGTTC TTTATATTCT AATGGAGTGT ATGGAGCGCC GTGCGTCTAT TACTATATTT 
GATGCAGAAT CTGAAGAACA GCAAGATATT GATTTGGCTA TATTGACAGC TCTGCTAAAA 
GGGAACCTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012827121.1
CDS	321..1532
Translation	MKVQLKSQKSWIEGLFNKRECAKIIPSSKEPHRCHGGCQVCQNLIRCCCGRLIGDHPGLDYGWPINPSPQEREDEEWSILNHTKPSPTDAFGTINFQDGDHTYHAKYIRISYDTTLELLMHLMIKEWQMELPKLVISVHGGIQNFKLPSKIKQVFGKGLVKAAETTGAWILTEGINTGASKHVGDALKAHASQHLRKICAVGIPPWGAIENQQDLIGKDVVCLYQTLINPMSKLTSLNSMHSHFIMVDDGTVGKYGNEMKLRRNLEDFISLQKIHTRMGQGVPVVGLVIEGGPNVILMVWEYVRSTPPVPVVVCEGTGRAADILAFTHKHTTDTEQISPQLKEEILEMIQKTFNLGHRQSNHVLYILMECMERRASITIFDAESEEQQDIDLAILTALLKGNL

Target ORF information:

RefSeq Version	XM_012971667.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis transient receptor potential cation channel, subfamily M, member 6 (trpm6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971667.2

ORF Insert Sequence:

ATGAAAGTCC AGCTAAAGTC TCAGAAATCA TGGATTGAAG GGCTCTTCAA TAAAAGAGAA 
TGTGCAAAAA TCATACCGTC ATCAAAAGAG CCACACAGAT GCCACGGTGG ATGTCAGGTT 
TGCCAGAATT TAATAAGGTG TTGTTGTGGA CGGTTAATTG GAGACCACCC TGGGTTAGAT 
TATGGATGGC CAATTAATCC TTCTCCCCAA GAAAGAGAAG ACGAAGAGTG GAGTATTCTA 
AATCATACAA AACCAAGTCC CACTGATGCA TTTGGCACAA TAAATTTTCA AGATGGAGAT 
CACACCTATC ATGCAAAGTA CATAAGAATA TCCTATGATA CCACATTGGA ACTGTTAATG 
CATCTGATGA TTAAAGAATG GCAAATGGAA CTGCCCAAGC TTGTGATCTC TGTCCATGGG 
GGCATACAGA ATTTTAAACT TCCGTCCAAA ATAAAGCAAG TTTTTGGCAA AGGATTGGTG 
AAAGCTGCTG AGACAACAGG AGCATGGATC CTTACAGAGG GCATTAATAC TGGGGCCTCC 
AAACATGTGG GTGATGCTTT AAAAGCACAC GCTTCTCAAC ACCTGCGAAA AATCTGTGCA 
GTTGGCATAC CACCATGGGG AGCTATTGAG AATCAGCAGG ATCTCATTGG CAAAGACGTT 
GTGTGCCTGT ATCAGACACT GATAAATCCA ATGAGCAAAC TGACAAGTCT CAATAGCATG 
CACTCCCATT TTATAATGGT GGATGATGGC ACAGTTGGAA AGTATGGTAA TGAAATGAAA 
TTAAGGAGAA ACTTGGAAGA CTTCATTTCC CTTCAGAAGA TCCATACCCG GATGGGGCAA 
GGAGTGCCTG TGGTAGGGCT TGTTATAGAA GGTGGTCCAA ATGTCATCTT GATGGTATGG 
GAATATGTGA GGAGCACTCC ACCAGTACCA GTTGTAGTGT GTGAAGGAAC AGGCAGAGCA 
GCTGATATAT TGGCATTTAC TCACAAGCAC ACAACGGACA CAGAGCAGAT TAGTCCACAG 
CTGAAAGAAG AAATCTTAGA GATGATTCAG AAGACATTTA ATCTTGGCCA CAGACAATCA 
AACCATGTTC TTTATATTCT AATGGAGTGT ATGGAGCGCC GTGCGTCTAT TACTATATTT 
GATGCAGAAT CTGAAGAACA GCAAGATATT GATTTGGCTA TATTGACAGC TCTGCTAAAA 
GGGAACCTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpm6 ( XM_012971667.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012827121.1 Xenopus tropicalis transient receptor potential cation channel, subfamily M, member 6 (trpm6), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa33854
Clone ID Related Accession (Same CDS sequence)	XM_012971667.2 , XM_012971667.3
Accession Version	XM_012971667.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transient receptor potential cation channel subfamily M member 6 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012971667.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTCC AGCTAAAGTC TCAGAAATCA TGGATTGAAG GGCTCTTCAA TAAAAGAGAA 
TGTGCAAAAA TCATACCGTC ATCAAAAGAG CCACACAGAT GCCACGGTGG ATGTCAGGTT 
TGCCAGAATT TAATAAGGTG TTGTTGTGGA CGGTTAATTG GAGACCACCC TGGGTTAGAT 
TATGGATGGC CAATTAATCC TTCTCCCCAA GAAAGAGAAG ACGAAGAGTG GAGTATTCTA 
AATCATACAA AACCAAGTCC CACTGATGCA TTTGGCACAA TAAATTTTCA AGATGGAGAT 
CACACCTATC ATGCAAAGTA CATAAGAATA TCCTATGATA CCACATTGGA ACTGTTAATG 
CATCTGATGA TTAAAGAATG GCAAATGGAA CTGCCCAAGC TTGTGATCTC TGTCCATGGG 
GGCATACAGA ATTTTAAACT TCCGTCCAAA ATAAAGCAAG TTTTTGGCAA AGGATTGGTG 
AAAGCTGCTG AGACAACAGG AGCATGGATC CTTACAGAGG GCATTAATAC TGGGGCCTCC 
AAACATGTGG GTGATGCTTT AAAAGCACAC GCTTCTCAAC ACCTGCGAAA AATCTGTGCA 
GTTGGCATAC CACCATGGGG AGCTATTGAG AATCAGCAGG ATCTCATTGG CAAAGACGTT 
GTGTGCCTGT ATCAGACACT GATAAATCCA ATGAGCAAAC TGACAAGTCT CAATAGCATG 
CACTCCCATT TTATAATGGT GGATGATGGC ACAGTTGGAA AGTATGGTAA TGAAATGAAA 
TTAAGGAGAA ACTTGGAAGA CTTCATTTCC CTTCAGAAGA TCCATACCCG GATGGGGCAA 
GGAGTGCCTG TGGTAGGGCT TGTTATAGAA GGTGGTCCAA ATGTCATCTT GATGGTATGG 
GAATATGTGA GGAGCACTCC ACCAGTACCA GTTGTAGTGT GTGAAGGAAC AGGCAGAGCA 
GCTGATATAT TGGCATTTAC TCACAAGCAC ACAACGGACA CAGAGCAGAT TAGTCCACAG 
CTGAAAGAAG AAATCTTAGA GATGATTCAG AAGACATTTA ATCTTGGCCA CAGACAATCA 
AACCATGTTC TTTATATTCT AATGGAGTGT ATGGAGCGCC GTGCGTCTAT TACTATATTT 
GATGCAGAAT CTGAAGAACA GCAAGATATT GATTTGGCTA TATTGACAGC TCTGCTAAAA 
GGGAACCTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012827121.1
CDS	297..1508
Translation	MKVQLKSQKSWIEGLFNKRECAKIIPSSKEPHRCHGGCQVCQNLIRCCCGRLIGDHPGLDYGWPINPSPQEREDEEWSILNHTKPSPTDAFGTINFQDGDHTYHAKYIRISYDTTLELLMHLMIKEWQMELPKLVISVHGGIQNFKLPSKIKQVFGKGLVKAAETTGAWILTEGINTGASKHVGDALKAHASQHLRKICAVGIPPWGAIENQQDLIGKDVVCLYQTLINPMSKLTSLNSMHSHFIMVDDGTVGKYGNEMKLRRNLEDFISLQKIHTRMGQGVPVVGLVIEGGPNVILMVWEYVRSTPPVPVVVCEGTGRAADILAFTHKHTTDTEQISPQLKEEILEMIQKTFNLGHRQSNHVLYILMECMERRASITIFDAESEEQQDIDLAILTALLKGNL

Target ORF information:

RefSeq Version	XM_012971667.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis transient receptor potential cation channel, subfamily M, member 6 (trpm6), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971667.3

ORF Insert Sequence:

ATGAAAGTCC AGCTAAAGTC TCAGAAATCA TGGATTGAAG GGCTCTTCAA TAAAAGAGAA 
TGTGCAAAAA TCATACCGTC ATCAAAAGAG CCACACAGAT GCCACGGTGG ATGTCAGGTT 
TGCCAGAATT TAATAAGGTG TTGTTGTGGA CGGTTAATTG GAGACCACCC TGGGTTAGAT 
TATGGATGGC CAATTAATCC TTCTCCCCAA GAAAGAGAAG ACGAAGAGTG GAGTATTCTA 
AATCATACAA AACCAAGTCC CACTGATGCA TTTGGCACAA TAAATTTTCA AGATGGAGAT 
CACACCTATC ATGCAAAGTA CATAAGAATA TCCTATGATA CCACATTGGA ACTGTTAATG 
CATCTGATGA TTAAAGAATG GCAAATGGAA CTGCCCAAGC TTGTGATCTC TGTCCATGGG 
GGCATACAGA ATTTTAAACT TCCGTCCAAA ATAAAGCAAG TTTTTGGCAA AGGATTGGTG 
AAAGCTGCTG AGACAACAGG AGCATGGATC CTTACAGAGG GCATTAATAC TGGGGCCTCC 
AAACATGTGG GTGATGCTTT AAAAGCACAC GCTTCTCAAC ACCTGCGAAA AATCTGTGCA 
GTTGGCATAC CACCATGGGG AGCTATTGAG AATCAGCAGG ATCTCATTGG CAAAGACGTT 
GTGTGCCTGT ATCAGACACT GATAAATCCA ATGAGCAAAC TGACAAGTCT CAATAGCATG 
CACTCCCATT TTATAATGGT GGATGATGGC ACAGTTGGAA AGTATGGTAA TGAAATGAAA 
TTAAGGAGAA ACTTGGAAGA CTTCATTTCC CTTCAGAAGA TCCATACCCG GATGGGGCAA 
GGAGTGCCTG TGGTAGGGCT TGTTATAGAA GGTGGTCCAA ATGTCATCTT GATGGTATGG 
GAATATGTGA GGAGCACTCC ACCAGTACCA GTTGTAGTGT GTGAAGGAAC AGGCAGAGCA 
GCTGATATAT TGGCATTTAC TCACAAGCAC ACAACGGACA CAGAGCAGAT TAGTCCACAG 
CTGAAAGAAG AAATCTTAGA GATGATTCAG AAGACATTTA ATCTTGGCCA CAGACAATCA 
AACCATGTTC TTTATATTCT AATGGAGTGT ATGGAGCGCC GTGCGTCTAT TACTATATTT 
GATGCAGAAT CTGAAGAACA GCAAGATATT GATTTGGCTA TATTGACAGC TCTGCTAAAA 
GGGAACCTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpm6 ( XM_018089406.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017944895.1 Xenopus tropicalis transient receptor potential cation channel, subfamily M, member 6 (trpm6), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa42109
Clone ID Related Accession (Same CDS sequence)	XM_018089406.1
Accession Version	XM_018089406.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1200bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transient receptor potential cation channel subfamily M member 6 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTCC AGCTAAAGTC TCAGAAATCA TGGATTGAAG GGCTCTTCAA TAAAAGAGAA 
TGTGCAAAAA TCATACCGTC ATCAAAAGAG CCACACAGAT GCCACGGTGG ATGTCAGGTT 
TGCCAGAATT TAATAAGGTG TTGTTGTGGA CGGTTAATTG GAGACCACCC TGGGTTAGAT 
TATGGATGGC CAATTAATCC TTCTCCCCAA GAAAGAGAAG ACGAAGAGTG GAGTATTCTA 
AATCATACAA AACCAAGTCC CACTGATGCA TTTGGCACAA TAAATTTTCA AGATGGAGAT 
CACACCTATC ATGCAAAGTA CATAAGAATA TCCTATGATA CCACATTGGA ACTGTTAATG 
CATCTGATGA TTAAAGAATG GCAAATGGAA CTGCCCAAGC TTGTGATCTC TGTCCATGGG 
GGCATACAGA ATTTTAAACT TCCGTCCAAA ATAAAGCAAG TTTTTGGCAA AGGATTGGTG 
AAAGCTGCTG AGACAACAGG AGCATGGATC CTTACAGAGG GCATTAATAC TGGGGCCTCC 
AAACATGTGG GTGATGCTTT AAAAGCACAC GCTTCTCAAC ACCTGCGAAA AATCTGTGCA 
GTTGGCATAC CACCATGGGG AGCTATTGAG AATCAGCAGG ATCTCATTGG CAAAGACGTT 
GTGTGCCTGT ATCAGACACT GATAAATCCA ATGAGCAAAC TGACAAGTCT CAATAGCATG 
CACTCCCATT TTATAATGGT GGATGATGGC ACAGTTGGAA AGTATGGTAA TGAAATGAAA 
TTAAGGAGAA ACTTGGAAGA CTTCATTTCC CTTCAGAAGA TCCATACCCG GATGGGGCAA 
GGAGTGCCTG TGGTAGGGCT TGTTATAGAA GGTGGTCCAA ATGTCATCTT GATGGTATGG 
GAATATGTGA GGAGCACTCC ACCAGTACCA GTTGTAGTGT GTGAAGGAAC AGGCAGAGCA 
GCTGATATAT TGGCATTTAC TCACAAGCAC ACAACGGACA CAGAGCAGAT TAGTCCACAG 
CTGAAAGAAG AAATCTTAGA GATGATTCAG AAGACATTTA ATCTTGGCCA CAGACAATCA 
AACCATGTTC TTTATATTCT AATGGAGTGT ATGGAGCGCC GTGCGTCTAG GCATGCAATG 
TGGGATCCCA GCACAACAGG AGTCAATAAA CTTGTGTTCA CGTTGTTGGA AACAGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017944895.1
CDS	326..1525
Translation	MKVQLKSQKSWIEGLFNKRECAKIIPSSKEPHRCHGGCQVCQNLIRCCCGRLIGDHPGLDYGWPINPSPQEREDEEWSILNHTKPSPTDAFGTINFQDGDHTYHAKYIRISYDTTLELLMHLMIKEWQMELPKLVISVHGGIQNFKLPSKIKQVFGKGLVKAAETTGAWILTEGINTGASKHVGDALKAHASQHLRKICAVGIPPWGAIENQQDLIGKDVVCLYQTLINPMSKLTSLNSMHSHFIMVDDGTVGKYGNEMKLRRNLEDFISLQKIHTRMGQGVPVVGLVIEGGPNVILMVWEYVRSTPPVPVVVCEGTGRAADILAFTHKHTTDTEQISPQLKEEILEMIQKTFNLGHRQSNHVLYILMECMERRASRHAMWDPSTTGVNKLVFTLLETV

Target ORF information:

RefSeq Version	XM_018089406.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis transient receptor potential cation channel, subfamily M, member 6 (trpm6), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018089406.1

ORF Insert Sequence:

ATGAAAGTCC AGCTAAAGTC TCAGAAATCA TGGATTGAAG GGCTCTTCAA TAAAAGAGAA 
TGTGCAAAAA TCATACCGTC ATCAAAAGAG CCACACAGAT GCCACGGTGG ATGTCAGGTT 
TGCCAGAATT TAATAAGGTG TTGTTGTGGA CGGTTAATTG GAGACCACCC TGGGTTAGAT 
TATGGATGGC CAATTAATCC TTCTCCCCAA GAAAGAGAAG ACGAAGAGTG GAGTATTCTA 
AATCATACAA AACCAAGTCC CACTGATGCA TTTGGCACAA TAAATTTTCA AGATGGAGAT 
CACACCTATC ATGCAAAGTA CATAAGAATA TCCTATGATA CCACATTGGA ACTGTTAATG 
CATCTGATGA TTAAAGAATG GCAAATGGAA CTGCCCAAGC TTGTGATCTC TGTCCATGGG 
GGCATACAGA ATTTTAAACT TCCGTCCAAA ATAAAGCAAG TTTTTGGCAA AGGATTGGTG 
AAAGCTGCTG AGACAACAGG AGCATGGATC CTTACAGAGG GCATTAATAC TGGGGCCTCC 
AAACATGTGG GTGATGCTTT AAAAGCACAC GCTTCTCAAC ACCTGCGAAA AATCTGTGCA 
GTTGGCATAC CACCATGGGG AGCTATTGAG AATCAGCAGG ATCTCATTGG CAAAGACGTT 
GTGTGCCTGT ATCAGACACT GATAAATCCA ATGAGCAAAC TGACAAGTCT CAATAGCATG 
CACTCCCATT TTATAATGGT GGATGATGGC ACAGTTGGAA AGTATGGTAA TGAAATGAAA 
TTAAGGAGAA ACTTGGAAGA CTTCATTTCC CTTCAGAAGA TCCATACCCG GATGGGGCAA 
GGAGTGCCTG TGGTAGGGCT TGTTATAGAA GGTGGTCCAA ATGTCATCTT GATGGTATGG 
GAATATGTGA GGAGCACTCC ACCAGTACCA GTTGTAGTGT GTGAAGGAAC AGGCAGAGCA 
GCTGATATAT TGGCATTTAC TCACAAGCAC ACAACGGACA CAGAGCAGAT TAGTCCACAG 
CTGAAAGAAG AAATCTTAGA GATGATTCAG AAGACATTTA ATCTTGGCCA CAGACAATCA 
AACCATGTTC TTTATATTCT AATGGAGTGT ATGGAGCGCC GTGCGTCTAG GCATGCAATG 
TGGGATCCCA GCACAACAGG AGTCAATAAA CTTGTGTTCA CGTTGTTGGA AACAGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpm6 ( XM_031890880.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746740.1 Xenopus tropicalis transient receptor potential cation channel, subfamily M, member 6 (trpm6), transcript variant X3, mRNA.

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CloneID	OXa48739
Clone ID Related Accession (Same CDS sequence)	XM_031890880.1
Accession Version	XM_031890880.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transient receptor potential cation channel subfamily M member 6 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCAGA AGACATTTAA TCTTGGCCAC AGACAATCAA ACCATGTTCT TTATATTCTA 
ATGGAGTGTA TGGAGCGCCG TGCGTCTATT ACTATATTTG ATGCAGAATC TGAAGAACAG 
CAAGATATTG ATTTGGCTAT ATTGACAGCT CTGCTAAAAG GGACTAATAT GTCTGCTCAA 
GACCAGCTGA GCTTGGCGCT AACCTGGAAC AGGGTTGACA TTGCCAAAAA ACACATACTT 
ATATATGGAC AACATTGGAA GGTGGGGTCT TTGGAACAAG CAATGCAAGA TGCACTTGTA 
ATGGATCGGG TGGATTTTGT AAAGTTGCTA ATTGAATATG GTGTGAATAT GCATCGCTTC 
TTGACCATTG GTCATTTGGA AGAATTATAC AATACTAAAC AAGGCCCCAC AAATCTGCTC 
CTACACCATT TAGTCAGAGA TGTAAAACAG AGCAATCCTT CTCCAGATTA CAGAATTTCC 
CTTATAGATG TGGGACTTGT AATCGAGTAC TTGATTGGTG GAGCCTACCG AAGCAGTTAT 
ACAAGGAAAA GTTTCAGAGT CCTGTACAAC AGTCTCTTCA GGAAGCATAA GAAATCTGTG 
CCCAGTTCCT CCAGCAGCCT TGCACAAAGT TTATCTCATC TTTCCATAAA CCAGAGCAGC 
CAGATGAGCA GCAAGCTTGA ATCGCCAGAG AGTACCTTGC ATTCCCAATT TTTCCGAACA 
GCCCAACCTT GTAGACGCAA GGACAAGACA ACATGCCATC AAAAGTCAAA AAAGAAAGAT 
GAAGTAACTT GCACAGAAGA TAATGAACCA GTTGGCTTTA TCTACCCTTA CAATGACCTC 
TTGGTTTGGG CTGTACTGAT GAAAAGACAG AAGATGGCTA TGTTTTTTTG GCAACATGGA 
GAGGAGGCCA TGGTCAAAGC AGTAATTGCC TGTAAGCTAT ATAGGGCCAT GGCACATGAA 
GCTAAGCAGA GCACCATGGT GGATGACACT TCAGAAGAAT TAAAGAAATA TTCAAAAGAA 
TTTGGACAGC TTGCTTTGGA CATACTTGAA AATGCTTTCA AAATGAATGA ACAAATGGCC 
ATGAAACTTT TAACATACCA GTTAAAGAAC TGGAGTAATT CAACGTGCCT GAAATTAGCG 
GTTTCATCAA CATTGCGGCC CTTTGTATCC CATACATGCA CACAGATGTT ACTGACAGAC 
ATGTGGATGG GTCGCCTGAA GATGCGGAAA CACTCCTGGC TTAAAGTCAT TCTAGGAATA 
TTGCTGCCAC CCTTTGTCTT GATGTTGGAA TTTAAGACCA AGGCAGAAAT GTCCCATGTA 
CCCCAGTCAC TAGAGTTCCA TCAGTTCTCA CGGCTGTACG TTGACCCTCC ATCACTGAGC 
ACCAAAGAGG AACATATTAC AGATTTTGAT GCTGAAAGGG GATCAAAGCA GCCAGGAGAA 
GATCAAAATC AGAAGATCAG TAATGGTCTA GGAATCCTTC CGATAACCAG GAAAATTTGT 
GAATTTTATA GCGCTCCCAT TGTCAAGTTC TGGTTCCACA CTATGGTGTA CTTGGCATTC 
CTCATGCTTT TCACATACAC GGTGCTGGTA AAGATGGACC CACAGCCCAG TGTCCAAGAG 
TGGTGTGTAA TAATTTATAT ATTCACTACG GCAGTTGAAA AAGTCAGGGA GGTATTTGTG 
TCAGAACCTG GAAAGTTCAG TCAAAAAGTA AAAGTTTGGA TCAATGAGTA CTGGAATCTT 
ACAGATTCTA TTGCAATTTT CTTGTTTTTT ATTGGTTTCG GCTTGCGCTG GCCCGACCCT 
CCGTTTCAAA CAGCAGGGCG CATAATATAT TGCCTGGACA TAATATTTTG GTATGTGCGA 
CTTTTAGACT TCTTTGCAGT GAACCAGCAA GCAGGCCCTT ACATTACAAT GCTTGGAAAA 
ATGACATCCA ACATGTTTGG TATTGTGATT ATAATGGCAA TTGTTCTGTT GGCATTTGGG 
GTGTCCCGGA AAGCAATTCT GTCTCCCGAT CACCCTCCTT CTTGGACTCT TGCTCGTGAT 
ATAGTGTTTC AGCCATACTG GATGATGTTT GGGGAAGTAT ATGCTGGGGA AATTGATGCC 
TGTGAAGAAG ATCCAGATTG CCCTCCTGGT TCCTTCATCA CCCCATTCCT TCAAGCTGTG 
TATCTCTTTG TGCAGTATAT CATCATGGTC AACCTGCTTA TTTCTCTGTT TAATAATGTC 
TACAGGGTAT TAAACTCTGT CTCTCACAAA CTTTGGAAAT ACAATCGATA TCGATATATA 
ATGACATACT ACGAGAAGCC ATGGCTACCT CCACCCCTGA TATTCCTTAG TCATATATAT 
ATACTTATCA ATCGCCTTTG TCATCACCGA TCTCACAGTG AGTTTGACCC AGAGGAAACT 
GACATGGGAC TGAAGCTGTA CCTTAGTGAT AATGAATTGA GGAAACTCCA TGATTTTGAG 
GAAAAATGTG TGGAAACATA CTTCCATGAG AAAAATGAAA GCCTGCACTC CAGCAAAAAT 
GAAAGAATCC GTGTAACAAC AGAGAGAACT GAAGAGATGT TTGCCCAACT GAAAGAAGTT 
AATGATAAAG TTTTCTACAT CAAAGACTCC TTGAGCTCCC TGGACAGCCA ATTAGGGCAT 
TTGCAGGATC TCTCCGCATT GACTGTGGAT ACTTTAAAAA TCATTTCAGC TGTGGATTCT 
TTGCAAGTTG AAGAAGCCTT TATAAAAGAC AAGAAAGGAC TCACCTGTAA AAAGCTCCCA 
CACAGTTGGA GCAACGTTCC ATATTCTCAT CACCAGAGAG AGTATAATTA CTACAGTATG 
CCACCTTCAC TTCTCCGGAC CTTGGCCAGG AGTCAGTGCC CATCACTGTA TCAATGTCCA 
CCACAGCAAC TGACTGCACA GTTAGAAATC CATAACTCAG AAGAACCCAA GATAGAGGTT 
GATATTGTTA CTATGGCAGT GCCATTGGAT AGTAAATCAG CTCAGCATTA CGGCAATTAC 
TTGCTTGTAC CTTCAGTGGG GCCCCCTGCA CCAACTGATT CTGCCTTTAA AGAGACATGT 
TCAGATATGC TCAGTGAAGG GGAAGCAATA TCTGTACAAG CTACAACCAA AGAGAGGATA 
TGTTCCATTC ATTCTGACCA GTGTCTCTGT AGCAAACAAA AGCATGTGAA GGAAAGTAGT 
CGCAAGACCA TGAAAGAGGA TTTAAAGCCT CTTGTGACAA ATACTGATGA AAGCCAAAAT 
GAAGAAAGCC ATGAATGCTC TTGCGATCAG TTTACTGCTC ATGTCACTGT TGAGACCTCA 
ATTACGTGCA ACATTATTGA CCCCGGAGGT GGTTATGTAA ACTTGGCATT TTCAGAAGAA 
GGAGAAACTG CCCTGTTTAG TGGAGGGAGT AGCAAGACCC TTTGCTCACG TCCCACCCAA 
AGTTCAAAAT CTAATGTGTT TCCAAATAAG TCTGATGGAT CTAGAGCCAA TGATAGGCCT 
GAGTTTGGTT CAGGTTACAA ACAGAAAACT TCATTCCTGA AAAATTCACT TTATAGGAAT 
AACTCACCAA GAGGCAGTTC TAAGAGGCAT AAAGAGAAGA AAGATGGAAA GATATGTACA 
ATCAAAGATT TTGCCAAGCC TCCTGAGAGC TCTAAGGTTA CATGGGCCAA AGAGAAACTG 
CGTAGCACAT TCAGAAAGTC TGTTAAAGAC AAGCCAAGGC CAACTCAACT TCAAGTTCCA 
GTGATAACTG TGGATGGTTG CTCTCAGAGT ACGCAGATGA GTTCCGAACC TGCAGATTGT 
GGCAGCTGGA AATCTGAAGA TGAACCCAGT AAGAACAGGC TTACAGTATC AAGTTTCAAT 
CAGCTAAGCT TGGATCACAT GACTTACATG CGACAGCAAC TAAAGACGCA AGAATTTGAA 
AGACACCTAG TTCAGACTAG TGAATATCTA AGGCAGTCCA GAGAGGATTT GCATAACACC 
ACCCAACTGA GAAGGAGCTC ATTATTCCGA AATTCCGATG AAGTAAAGAA AATGGCAGCT 
TCCCTAAAAG CCTCTCAGGA TTTAAGTAAT CATTATTCAG CGGTGGAAAG GAATAATTTA 
ATGAGGCTTG CACAGAGCAT ACCTTTCACC CCCCTGCACC TTTTTGCTGG AGAAGAAGTC 
ACTGTTTACC AACTAGAGGA AAGTTCTCCA GCAATTCTAG ATAAAAGTAT GTCTTCCTGG 
TCACAGCGTG GAATGGCAGC AATGCTTCAG GTTCTGTCCC CAGAGGAAAT GGATGGAGGT 
CTCCGCAGGG CGTTAAAAGT TGTGTGCACT TGGTCTGAGC ATGATGTACT GACGCCTGGT 
GAGGTGTTTA TAGTCAAATC TTTTCTTCCG GAGGTAGTAC AGACTTGGCA GAAGGTTTTC 
CGTGGTGATA CTGTACTGAA TCTGTGTCTC AGGGAAATTC AGCAACAAAG AGCAGCTCAA 
AAGTTGATCT ACACCTTCAA TCAAGTGAAA CCACACAGTA TTCCTTATAC TCTAAGGTTT 
CTAGAAGTAT TTCTTATATT CTGCCATTCA GCTAACCAAT GGCTTACTAT AGAGAAGTAC 
CTGACTGGAG AATTCCGCAA GTACAATAAC AACAATGGAG ATGAAATTAC CCCCACCACT 
TTACTTGAGG AGACACTGCT GTCTTTTTCT CATTGGAGTT ATGAATATAC AAGGGGAGAG 
CTGCTTGTTT TGGATTTACA AGGCGTTGGG GAAAATCTTA CAGACCCATC TGTTATTAAA 
CCAGAAGACA AACAATCAAG AGGAATGGTA TTTGGACCAG CTAATTTAGG AGAAGATGCT 
CTGACTAATT ATGTTTCAAA GCATCGCTGC AATTCCTGCT GCAGAAAGCT TAAACTCCTT 
GTGCACTCAT TCTGTCTCAC CTTGTTTTTG AGAACAGTAG CAGCTGTAGC AGCCGAAACG 
TTAGATGTAT TCCTATCAAT AAATGTTTTG ATTGACCATA AGTCCTATTT GAAAAGAAAT 
GACTACTCAC CTGGGAGGAT CAATCTAGGG TTTGAAATTG GAGAAGCAGA CACAGATAAA 
GACCTTGTGG AATATAATAC AAGACTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746740.1
CDS	127..5196
Translation	MIQKTFNLGHRQSNHVLYILMECMERRASITIFDAESEEQQDIDLAILTALLKGTNMSAQDQLSLALTWNRVDIAKKHILIYGQHWKVGSLEQAMQDALVMDRVDFVKLLIEYGVNMHRFLTIGHLEELYNTKQGPTNLLLHHLVRDVKQSNPSPDYRISLIDVGLVIEYLIGGAYRSSYTRKSFRVLYNSLFRKHKKSVPSSSSSLAQSLSHLSINQSSQMSSKLESPESTLHSQFFRTAQPCRRKDKTTCHQKSKKKDEVTCTEDNEPVGFIYPYNDLLVWAVLMKRQKMAMFFWQHGEEAMVKAVIACKLYRAMAHEAKQSTMVDDTSEELKKYSKEFGQLALDILENAFKMNEQMAMKLLTYQLKNWSNSTCLKLAVSSTLRPFVSHTCTQMLLTDMWMGRLKMRKHSWLKVILGILLPPFVLMLEFKTKAEMSHVPQSLEFHQFSRLYVDPPSLSTKEEHITDFDAERGSKQPGEDQNQKISNGLGILPITRKICEFYSAPIVKFWFHTMVYLAFLMLFTYTVLVKMDPQPSVQEWCVIIYIFTTAVEKVREVFVSEPGKFSQKVKVWINEYWNLTDSIAIFLFFIGFGLRWPDPPFQTAGRIIYCLDIIFWYVRLLDFFAVNQQAGPYITMLGKMTSNMFGIVIIMAIVLLAFGVSRKAILSPDHPPSWTLARDIVFQPYWMMFGEVYAGEIDACEEDPDCPPGSFITPFLQAVYLFVQYIIMVNLLISLFNNVYRVLNSVSHKLWKYNRYRYIMTYYEKPWLPPPLIFLSHIYILINRLCHHRSHSEFDPEETDMGLKLYLSDNELRKLHDFEEKCVETYFHEKNESLHSSKNERIRVTTERTEEMFAQLKEVNDKVFYIKDSLSSLDSQLGHLQDLSALTVDTLKIISAVDSLQVEEAFIKDKKGLTCKKLPHSWSNVPYSHHQREYNYYSMPPSLLRTLARSQCPSLYQCPPQQLTAQLEIHNSEEPKIEVDIVTMAVPLDSKSAQHYGNYLLVPSVGPPAPTDSAFKETCSDMLSEGEAISVQATTKERICSIHSDQCLCSKQKHVKESSRKTMKEDLKPLVTNTDESQNEESHECSCDQFTAHVTVETSITCNIIDPGGGYVNLAFSEEGETALFSGGSSKTLCSRPTQSSKSNVFPNKSDGSRANDRPEFGSGYKQKTSFLKNSLYRNNSPRGSSKRHKEKKDGKICTIKDFAKPPESSKVTWAKEKLRSTFRKSVKDKPRPTQLQVPVITVDGCSQSTQMSSEPADCGSWKSEDEPSKNRLTVSSFNQLSLDHMTYMRQQLKTQEFERHLVQTSEYLRQSREDLHNTTQLRRSSLFRNSDEVKKMAASLKASQDLSNHYSAVERNNLMRLAQSIPFTPLHLFAGEEVTVYQLEESSPAILDKSMSSWSQRGMAAMLQVLSPEEMDGGLRRALKVVCTWSEHDVLTPGEVFIVKSFLPEVVQTWQKVFRGDTVLNLCLREIQQQRAAQKLIYTFNQVKPHSIPYTLRFLEVFLIFCHSANQWLTIEKYLTGEFRKYNNNNGDEITPTTLLEETLLSFSHWSYEYTRGELLVLDLQGVGENLTDPSVIKPEDKQSRGMVFGPANLGEDALTNYVSKHRCNSCCRKLKLLVHSFCLTLFLRTVAAVAAETLDVFLSINVLIDHKSYLKRNDYSPGRINLGFEIGEADTDKDLVEYNTRL

Target ORF information:

RefSeq Version	XM_031890880.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis transient receptor potential cation channel, subfamily M, member 6 (trpm6), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031890880.1

ORF Insert Sequence:

ATGATTCAGA AGACATTTAA TCTTGGCCAC AGACAATCAA ACCATGTTCT TTATATTCTA 
ATGGAGTGTA TGGAGCGCCG TGCGTCTATT ACTATATTTG ATGCAGAATC TGAAGAACAG 
CAAGATATTG ATTTGGCTAT ATTGACAGCT CTGCTAAAAG GGACTAATAT GTCTGCTCAA 
GACCAGCTGA GCTTGGCGCT AACCTGGAAC AGGGTTGACA TTGCCAAAAA ACACATACTT 
ATATATGGAC AACATTGGAA GGTGGGGTCT TTGGAACAAG CAATGCAAGA TGCACTTGTA 
ATGGATCGGG TGGATTTTGT AAAGTTGCTA ATTGAATATG GTGTGAATAT GCATCGCTTC 
TTGACCATTG GTCATTTGGA AGAATTATAC AATACTAAAC AAGGCCCCAC AAATCTGCTC 
CTACACCATT TAGTCAGAGA TGTAAAACAG AGCAATCCTT CTCCAGATTA CAGAATTTCC 
CTTATAGATG TGGGACTTGT AATCGAGTAC TTGATTGGTG GAGCCTACCG AAGCAGTTAT 
ACAAGGAAAA GTTTCAGAGT CCTGTACAAC AGTCTCTTCA GGAAGCATAA GAAATCTGTG 
CCCAGTTCCT CCAGCAGCCT TGCACAAAGT TTATCTCATC TTTCCATAAA CCAGAGCAGC 
CAGATGAGCA GCAAGCTTGA ATCGCCAGAG AGTACCTTGC ATTCCCAATT TTTCCGAACA 
GCCCAACCTT GTAGACGCAA GGACAAGACA ACATGCCATC AAAAGTCAAA AAAGAAAGAT 
GAAGTAACTT GCACAGAAGA TAATGAACCA GTTGGCTTTA TCTACCCTTA CAATGACCTC 
TTGGTTTGGG CTGTACTGAT GAAAAGACAG AAGATGGCTA TGTTTTTTTG GCAACATGGA 
GAGGAGGCCA TGGTCAAAGC AGTAATTGCC TGTAAGCTAT ATAGGGCCAT GGCACATGAA 
GCTAAGCAGA GCACCATGGT GGATGACACT TCAGAAGAAT TAAAGAAATA TTCAAAAGAA 
TTTGGACAGC TTGCTTTGGA CATACTTGAA AATGCTTTCA AAATGAATGA ACAAATGGCC 
ATGAAACTTT TAACATACCA GTTAAAGAAC TGGAGTAATT CAACGTGCCT GAAATTAGCG 
GTTTCATCAA CATTGCGGCC CTTTGTATCC CATACATGCA CACAGATGTT ACTGACAGAC 
ATGTGGATGG GTCGCCTGAA GATGCGGAAA CACTCCTGGC TTAAAGTCAT TCTAGGAATA 
TTGCTGCCAC CCTTTGTCTT GATGTTGGAA TTTAAGACCA AGGCAGAAAT GTCCCATGTA 
CCCCAGTCAC TAGAGTTCCA TCAGTTCTCA CGGCTGTACG TTGACCCTCC ATCACTGAGC 
ACCAAAGAGG AACATATTAC AGATTTTGAT GCTGAAAGGG GATCAAAGCA GCCAGGAGAA 
GATCAAAATC AGAAGATCAG TAATGGTCTA GGAATCCTTC CGATAACCAG GAAAATTTGT 
GAATTTTATA GCGCTCCCAT TGTCAAGTTC TGGTTCCACA CTATGGTGTA CTTGGCATTC 
CTCATGCTTT TCACATACAC GGTGCTGGTA AAGATGGACC CACAGCCCAG TGTCCAAGAG 
TGGTGTGTAA TAATTTATAT ATTCACTACG GCAGTTGAAA AAGTCAGGGA GGTATTTGTG 
TCAGAACCTG GAAAGTTCAG TCAAAAAGTA AAAGTTTGGA TCAATGAGTA CTGGAATCTT 
ACAGATTCTA TTGCAATTTT CTTGTTTTTT ATTGGTTTCG GCTTGCGCTG GCCCGACCCT 
CCGTTTCAAA CAGCAGGGCG CATAATATAT TGCCTGGACA TAATATTTTG GTATGTGCGA 
CTTTTAGACT TCTTTGCAGT GAACCAGCAA GCAGGCCCTT ACATTACAAT GCTTGGAAAA 
ATGACATCCA ACATGTTTGG TATTGTGATT ATAATGGCAA TTGTTCTGTT GGCATTTGGG 
GTGTCCCGGA AAGCAATTCT GTCTCCCGAT CACCCTCCTT CTTGGACTCT TGCTCGTGAT 
ATAGTGTTTC AGCCATACTG GATGATGTTT GGGGAAGTAT ATGCTGGGGA AATTGATGCC 
TGTGAAGAAG ATCCAGATTG CCCTCCTGGT TCCTTCATCA CCCCATTCCT TCAAGCTGTG 
TATCTCTTTG TGCAGTATAT CATCATGGTC AACCTGCTTA TTTCTCTGTT TAATAATGTC 
TACAGGGTAT TAAACTCTGT CTCTCACAAA CTTTGGAAAT ACAATCGATA TCGATATATA 
ATGACATACT ACGAGAAGCC ATGGCTACCT CCACCCCTGA TATTCCTTAG TCATATATAT 
ATACTTATCA ATCGCCTTTG TCATCACCGA TCTCACAGTG AGTTTGACCC AGAGGAAACT 
GACATGGGAC TGAAGCTGTA CCTTAGTGAT AATGAATTGA GGAAACTCCA TGATTTTGAG 
GAAAAATGTG TGGAAACATA CTTCCATGAG AAAAATGAAA GCCTGCACTC CAGCAAAAAT 
GAAAGAATCC GTGTAACAAC AGAGAGAACT GAAGAGATGT TTGCCCAACT GAAAGAAGTT 
AATGATAAAG TTTTCTACAT CAAAGACTCC TTGAGCTCCC TGGACAGCCA ATTAGGGCAT 
TTGCAGGATC TCTCCGCATT GACTGTGGAT ACTTTAAAAA TCATTTCAGC TGTGGATTCT 
TTGCAAGTTG AAGAAGCCTT TATAAAAGAC AAGAAAGGAC TCACCTGTAA AAAGCTCCCA 
CACAGTTGGA GCAACGTTCC ATATTCTCAT CACCAGAGAG AGTATAATTA CTACAGTATG 
CCACCTTCAC TTCTCCGGAC CTTGGCCAGG AGTCAGTGCC CATCACTGTA TCAATGTCCA 
CCACAGCAAC TGACTGCACA GTTAGAAATC CATAACTCAG AAGAACCCAA GATAGAGGTT 
GATATTGTTA CTATGGCAGT GCCATTGGAT AGTAAATCAG CTCAGCATTA CGGCAATTAC 
TTGCTTGTAC CTTCAGTGGG GCCCCCTGCA CCAACTGATT CTGCCTTTAA AGAGACATGT 
TCAGATATGC TCAGTGAAGG GGAAGCAATA TCTGTACAAG CTACAACCAA AGAGAGGATA 
TGTTCCATTC ATTCTGACCA GTGTCTCTGT AGCAAACAAA AGCATGTGAA GGAAAGTAGT 
CGCAAGACCA TGAAAGAGGA TTTAAAGCCT CTTGTGACAA ATACTGATGA AAGCCAAAAT 
GAAGAAAGCC ATGAATGCTC TTGCGATCAG TTTACTGCTC ATGTCACTGT TGAGACCTCA 
ATTACGTGCA ACATTATTGA CCCCGGAGGT GGTTATGTAA ACTTGGCATT TTCAGAAGAA 
GGAGAAACTG CCCTGTTTAG TGGAGGGAGT AGCAAGACCC TTTGCTCACG TCCCACCCAA 
AGTTCAAAAT CTAATGTGTT TCCAAATAAG TCTGATGGAT CTAGAGCCAA TGATAGGCCT 
GAGTTTGGTT CAGGTTACAA ACAGAAAACT TCATTCCTGA AAAATTCACT TTATAGGAAT 
AACTCACCAA GAGGCAGTTC TAAGAGGCAT AAAGAGAAGA AAGATGGAAA GATATGTACA 
ATCAAAGATT TTGCCAAGCC TCCTGAGAGC TCTAAGGTTA CATGGGCCAA AGAGAAACTG 
CGTAGCACAT TCAGAAAGTC TGTTAAAGAC AAGCCAAGGC CAACTCAACT TCAAGTTCCA 
GTGATAACTG TGGATGGTTG CTCTCAGAGT ACGCAGATGA GTTCCGAACC TGCAGATTGT 
GGCAGCTGGA AATCTGAAGA TGAACCCAGT AAGAACAGGC TTACAGTATC AAGTTTCAAT 
CAGCTAAGCT TGGATCACAT GACTTACATG CGACAGCAAC TAAAGACGCA AGAATTTGAA 
AGACACCTAG TTCAGACTAG TGAATATCTA AGGCAGTCCA GAGAGGATTT GCATAACACC 
ACCCAACTGA GAAGGAGCTC ATTATTCCGA AATTCCGATG AAGTAAAGAA AATGGCAGCT 
TCCCTAAAAG CCTCTCAGGA TTTAAGTAAT CATTATTCAG CGGTGGAAAG GAATAATTTA 
ATGAGGCTTG CACAGAGCAT ACCTTTCACC CCCCTGCACC TTTTTGCTGG AGAAGAAGTC 
ACTGTTTACC AACTAGAGGA AAGTTCTCCA GCAATTCTAG ATAAAAGTAT GTCTTCCTGG 
TCACAGCGTG GAATGGCAGC AATGCTTCAG GTTCTGTCCC CAGAGGAAAT GGATGGAGGT 
CTCCGCAGGG CGTTAAAAGT TGTGTGCACT TGGTCTGAGC ATGATGTACT GACGCCTGGT 
GAGGTGTTTA TAGTCAAATC TTTTCTTCCG GAGGTAGTAC AGACTTGGCA GAAGGTTTTC 
CGTGGTGATA CTGTACTGAA TCTGTGTCTC AGGGAAATTC AGCAACAAAG AGCAGCTCAA 
AAGTTGATCT ACACCTTCAA TCAAGTGAAA CCACACAGTA TTCCTTATAC TCTAAGGTTT 
CTAGAAGTAT TTCTTATATT CTGCCATTCA GCTAACCAAT GGCTTACTAT AGAGAAGTAC 
CTGACTGGAG AATTCCGCAA GTACAATAAC AACAATGGAG ATGAAATTAC CCCCACCACT 
TTACTTGAGG AGACACTGCT GTCTTTTTCT CATTGGAGTT ATGAATATAC AAGGGGAGAG 
CTGCTTGTTT TGGATTTACA AGGCGTTGGG GAAAATCTTA CAGACCCATC TGTTATTAAA 
CCAGAAGACA AACAATCAAG AGGAATGGTA TTTGGACCAG CTAATTTAGG AGAAGATGCT 
CTGACTAATT ATGTTTCAAA GCATCGCTGC AATTCCTGCT GCAGAAAGCT TAAACTCCTT 
GTGCACTCAT TCTGTCTCAC CTTGTTTTTG AGAACAGTAG CAGCTGTAGC AGCCGAAACG 
TTAGATGTAT TCCTATCAAT AAATGTTTTG ATTGACCATA AGTCCTATTT GAAAAGAAAT 
GACTACTCAC CTGGGAGGAT CAATCTAGGG TTTGAAATTG GAGAAGCAGA CACAGATAAA 
GACCTTGTGG AATATAATAC AAGACTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpm6 ( XM_031890885.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746745.1 Xenopus tropicalis transient receptor potential cation channel, subfamily M, member 6 (trpm6), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa48740
Clone ID Related Accession (Same CDS sequence)	XM_031890885.1
Accession Version	XM_031890885.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transient receptor potential cation channel subfamily M member 6 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTC AAGACCAGCT GAGCTTGGCG CTAACCTGGA ACAGGGTTGA CATTGCCAAA 
AAACACATAC TTATATATGG ACAACATTGG AAGGTGGGGT CTTTGGAACA AGCAATGCAA 
GATGCACTTG TAATGGATCG GGTGGATTTT GTAAAGTTGC TAATTGAATA TGGTGTGAAT 
ATGCATCGCT TCTTGACCAT TGGTCATTTG GAAGAATTAT ACAATACTAA ACAAGGCCCC 
ACAAATCTGC TCCTACACCA TTTAGTCAGA GATGTAAAAC AGAGCAATCC TTCTCCAGAT 
TACAGAATTT CCCTTATAGA TGTGGGACTT GTAATCGAGT ACTTGATTGG TGGAGCCTAC 
CGAAGCAGTT ATACAAGGAA AAGTTTCAGA GTCCTGTACA ACAGTCTCTT CAGGAAGCAT 
AAGAAATCTG TGCCCAGTTC CTCCAGCAGC CTTGCACAAA GTTTATCTCA TCTTTCCATA 
AACCAGAGCA GCCAGATGAG CAGCAAGCTT GAATCGCCAG AGAGTACCTT GCATTCCCAA 
TTTTTCCGAA CAGCCCAACC TTGTAGACGC AAGGACAAGA CAACATGCCA TCAAAAGTCA 
AAAAAGAAAG ATGAAGTAAC TTGCACAGAA GATAATGAAC CAGTTGGCTT TATCTACCCT 
TACAATGACC TCTTGGTTTG GGCTGTACTG ATGAAAAGAC AGAAGATGGC TATGTTTTTT 
TGGCAACATG GAGAGGAGGC CATGGTCAAA GCAGTAATTG CCTGTAAGCT ATATAGGGCC 
ATGGCACATG AAGCTAAGCA GAGCACCATG GTGGATGACA CTTCAGAAGA ATTAAAGAAA 
TATTCAAAAG AATTTGGACA GCTTGCTTTG GACATACTTG AAAATGCTTT CAAAATGAAT 
GAACAAATGG CCATGAAACT TTTAACATAC CAGTTAAAGA ACTGGAGTAA TTCAACGTGC 
CTGAAATTAG CGGTTTCATC AACATTGCGG CCCTTTGTAT CCCATACATG CACACAGATG 
TTACTGACAG ACATGTGGAT GGGTCGCCTG AAGATGCGGA AACACTCCTG GCTTAAAGTC 
ATTCTAGGAA TATTGCTGCC ACCCTTTGTC TTGATGTTGG AATTTAAGAC CAAGGCAGAA 
ATGTCCCATG TACCCCAGTC ACTAGAGTTC CATCAGTTCT CACGGCTGTA CGTTGACCCT 
CCATCACTGA GCACCAAAGA GGAACATATT ACAGATTTTG ATGCTGAAAG GGGATCAAAG 
CAGCCAGGAG AAGATCAAAA TCAGAAGATC AGTAATGGTC TAGGAATCCT TCCGATAACC 
AGGAAAATTT GTGAATTTTA TAGCGCTCCC ATTGTCAAGT TCTGGTTCCA CACTATGGTG 
TACTTGGCAT TCCTCATGCT TTTCACATAC ACGGTGCTGG TAAAGATGGA CCCACAGCCC 
AGTGTCCAAG AGTGGTGTGT AATAATTTAT ATATTCACTA CGGCAGTTGA AAAAGTCAGG 
GAGGTATTTG TGTCAGAACC TGGAAAGTTC AGTCAAAAAG TAAAAGTTTG GATCAATGAG 
TACTGGAATC TTACAGATTC TATTGCAATT TTCTTGTTTT TTATTGGTTT CGGCTTGCGC 
TGGCCCGACC CTCCGTTTCA AACAGCAGGG CGCATAATAT ATTGCCTGGA CATAATATTT 
TGGTATGTGC GACTTTTAGA CTTCTTTGCA GTGAACCAGC AAGCAGGCCC TTACATTACA 
ATGCTTGGAA AAATGACATC CAACATGTTT GGTATTGTGA TTATAATGGC AATTGTTCTG 
TTGGCATTTG GGGTGTCCCG GAAAGCAATT CTGTCTCCCG ATCACCCTCC TTCTTGGACT 
CTTGCTCGTG ATATAGTGTT TCAGCCATAC TGGATGATGT TTGGGGAAGT ATATGCTGGG 
GAAATTGATG CCTGTGAAGA AGATCCAGAT TGCCCTCCTG GTTCCTTCAT CACCCCATTC 
CTTCAAGCTG TGTATCTCTT TGTGCAGTAT ATCATCATGG TCAACCTGCT TATTTCTCTG 
TTTAATAATG TCTACAGGGT ATTAAACTCT GTCTCTCACA AACTTTGGAA ATACAATCGA 
TATCGATATA TAATGACATA CTACGAGAAG CCATGGCTAC CTCCACCCCT GATATTCCTT 
AGTCATATAT ATATACTTAT CAATCGCCTT TGTCATCACC GATCTCACAG TGAGTTTGAC 
CCAGAGGAAA CTGACATGGG ACTGAAGCTG TACCTTAGTG ATAATGAATT GAGGAAACTC 
CATGATTTTG AGGAAAAATG TGTGGAAACA TACTTCCATG AGAAAAATGA AAGCCTGCAC 
TCCAGCAAAA ATGAAAGAAT CCGTGTAACA ACAGAGAGAA CTGAAGAGAT GTTTGCCCAA 
CTGAAAGAAG TTAATGATAA AGTTTTCTAC ATCAAAGACT CCTTGAGCTC CCTGGACAGC 
CAATTAGGGC ATTTGCAGGA TCTCTCCGCA TTGACTGTGG ATACTTTAAA AATCATTTCA 
GCTGTGGATT CTTTGCAAGT TGAAGAAGCC TTTATAAAAG ACAAGAAAGG ACTCACCTGT 
AAAAAGCTCC CACACAGTTG GAGCAACGTT CCATATTCTC ATCACCAGAG AGAGTATAAT 
TACTACAGTA TGCCACCTTC ACTTCTCCGG ACCTTGGCCA GGAGTCAGTG CCCATCACTG 
TATCAATGTC CACCACAGCA ACTGACTGCA CAGTTAGAAA TCCATAACTC AGAAGAACCC 
AAGATAGAGG TTGATATTGT TACTATGGCA GTGCCATTGG ATAGTAAATC AGCTCAGCAT 
TACGGCAATT ACTTGCTTGT ACCTTCAGTG GGGCCCCCTG CACCAACTGA TTCTGCCTTT 
AAAGAGACAT GTTCAGATAT GCTCAGTGAA GGGGAAGCAA TATCTGTACA AGCTACAACC 
AAAGAGAGGA TATGTTCCAT TCATTCTGAC CAGTGTCTCT GTAGCAAACA AAAGCATGTG 
AAGGAAAGTA GTCGCAAGAC CATGAAAGAG GATTTAAAGC CTCTTGTGAC AAATACTGAT 
GAAAGCCAAA ATGAAGAAAG CCATGAATGC TCTTGCGATC AGTTTACTGC TCATGTCACT 
GTTGAGACCT CAATTACGTG CAACATTATT GACCCCGGAG GTGGTTATGT AAACTTGGCA 
TTTTCAGAAG AAGGAGAAAC TGCCCTGTTT AGTGGAGGGA GTAGCAAGAC CCTTTGCTCA 
CGTCCCACCC AAAGTTCAAA ATCTAATGTG TTTCCAAATA AGTCTGATGG ATCTAGAGCC 
AATGATAGGC CTGAGTTTGG TTCAGGTTAC AAACAGAAAA CTTCATTCCT GAAAAATTCA 
CTTTATAGGA ATAACTCACC AAGAGGCAGT TCTAAGAGGC ATAAAGAGAA GAAAGATGGA 
AAGATATGTA CAATCAAAGA TTTTGCCAAG CCTCCTGAGA GCTCTAAGGT TACATGGGCC 
AAAGAGAAAC TGCGTAGCAC ATTCAGAAAG TCTGTTAAAG ACAAGCCAAG GCCAACTCAA 
CTTCAAGTTC CAGTGATAAC TGTGGATGGT TGCTCTCAGA GTACGCAGAT GAGTTCCGAA 
CCTGCAGATT GTGGCAGCTG GAAATCTGAA GATGAACCCA GTAAGAACAG GCTTACAGTA 
TCAAGTTTCA ATCAGCTAAG CTTGGATCAC ATGACTTACA TGCGACAGCA ACTAAAGACG 
CAAGAATTTG AAAGACACCT AGTTCAGACT AGTGAATATC TAAGGCAGTC CAGAGAGGAT 
TTGCATAACA CCACCCAACT GAGAAGGAGC TCATTATTCC GAAATTCCGA TGAAGTAAAG 
AAAATGGCAG CTTCCCTAAA AGCCTCTCAG GATTTAAGTA ATCATTATTC AGCGGTGGAA 
AGGAATAATT TAATGAGGCT TGCACAGAGC ATACCTTTCA CCCCCCTGCA CCTTTTTGCT 
GGAGAAGAAG TCACTGTTTA CCAACTAGAG GAAAGTTCTC CAGCAATTCT AGATAAAAGT 
ATGTCTTCCT GGTCACAGCG TGGAATGGCA GCAATGCTTC AGGTTCTGTC CCCAGAGGAA 
ATGGATGGAG GTCTCCGCAG GGCGTTAAAA GTTGTGTGCA CTTGGTCTGA GCATGATGTA 
CTGACGCCTG GTGAGGTGTT TATAGTCAAA TCTTTTCTTC CGGAGGTAGT ACAGACTTGG 
CAGAAGGTTT TCCGTGGTGA TACTGTACTG AATCTGTGTC TCAGGGAAAT TCAGCAACAA 
AGAGCAGCTC AAAAGTTGAT CTACACCTTC AATCAAGTGA AACCACACAG TATTCCTTAT 
ACTCTAAGGT TTCTAGAAGT ATTTCTTATA TTCTGCCATT CAGCTAACCA ATGGCTTACT 
ATAGAGAAGT ACCTGACTGG AGAATTCCGC AAGTACAATA ACAACAATGG AGATGAAATT 
ACCCCCACCA CTTTACTTGA GGAGACACTG CTGTCTTTTT CTCATTGGAG TTATGAATAT 
ACAAGGGGAG AGCTGCTTGT TTTGGATTTA CAAGGCGTTG GGGAAAATCT TACAGACCCA 
TCTGTTATTA AACCAGAAGA CAAACAATCA AGAGGAATGG TATTTGGACC AGCTAATTTA 
GGAGAAGATG CTCTGACTAA TTATGTTTCA AAGCATCGCT GCAATTCCTG CTGCAGAAAG 
CTTAAACTCC TTGTGCACTC ATTCTGTCTC ACCTTGTTTT TGAGAACAGT AGCAGCTGTA 
GCAGCCGAAA CGTTAGATGT ATTCCTATCA ATAAATGTTT TGATTGACCA TAAGTCCTAT 
TTGAAAAGAA ATGACTACTC ACCTGGGAGG ATCAATCTAG GGTTTGAAAT TGGAGAAGCA 
GACACAGATA AAGACCTTGT GGAATATAAT ACAAGACTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746745.1
CDS	108..5009
Translation	MSAQDQLSLALTWNRVDIAKKHILIYGQHWKVGSLEQAMQDALVMDRVDFVKLLIEYGVNMHRFLTIGHLEELYNTKQGPTNLLLHHLVRDVKQSNPSPDYRISLIDVGLVIEYLIGGAYRSSYTRKSFRVLYNSLFRKHKKSVPSSSSSLAQSLSHLSINQSSQMSSKLESPESTLHSQFFRTAQPCRRKDKTTCHQKSKKKDEVTCTEDNEPVGFIYPYNDLLVWAVLMKRQKMAMFFWQHGEEAMVKAVIACKLYRAMAHEAKQSTMVDDTSEELKKYSKEFGQLALDILENAFKMNEQMAMKLLTYQLKNWSNSTCLKLAVSSTLRPFVSHTCTQMLLTDMWMGRLKMRKHSWLKVILGILLPPFVLMLEFKTKAEMSHVPQSLEFHQFSRLYVDPPSLSTKEEHITDFDAERGSKQPGEDQNQKISNGLGILPITRKICEFYSAPIVKFWFHTMVYLAFLMLFTYTVLVKMDPQPSVQEWCVIIYIFTTAVEKVREVFVSEPGKFSQKVKVWINEYWNLTDSIAIFLFFIGFGLRWPDPPFQTAGRIIYCLDIIFWYVRLLDFFAVNQQAGPYITMLGKMTSNMFGIVIIMAIVLLAFGVSRKAILSPDHPPSWTLARDIVFQPYWMMFGEVYAGEIDACEEDPDCPPGSFITPFLQAVYLFVQYIIMVNLLISLFNNVYRVLNSVSHKLWKYNRYRYIMTYYEKPWLPPPLIFLSHIYILINRLCHHRSHSEFDPEETDMGLKLYLSDNELRKLHDFEEKCVETYFHEKNESLHSSKNERIRVTTERTEEMFAQLKEVNDKVFYIKDSLSSLDSQLGHLQDLSALTVDTLKIISAVDSLQVEEAFIKDKKGLTCKKLPHSWSNVPYSHHQREYNYYSMPPSLLRTLARSQCPSLYQCPPQQLTAQLEIHNSEEPKIEVDIVTMAVPLDSKSAQHYGNYLLVPSVGPPAPTDSAFKETCSDMLSEGEAISVQATTKERICSIHSDQCLCSKQKHVKESSRKTMKEDLKPLVTNTDESQNEESHECSCDQFTAHVTVETSITCNIIDPGGGYVNLAFSEEGETALFSGGSSKTLCSRPTQSSKSNVFPNKSDGSRANDRPEFGSGYKQKTSFLKNSLYRNNSPRGSSKRHKEKKDGKICTIKDFAKPPESSKVTWAKEKLRSTFRKSVKDKPRPTQLQVPVITVDGCSQSTQMSSEPADCGSWKSEDEPSKNRLTVSSFNQLSLDHMTYMRQQLKTQEFERHLVQTSEYLRQSREDLHNTTQLRRSSLFRNSDEVKKMAASLKASQDLSNHYSAVERNNLMRLAQSIPFTPLHLFAGEEVTVYQLEESSPAILDKSMSSWSQRGMAAMLQVLSPEEMDGGLRRALKVVCTWSEHDVLTPGEVFIVKSFLPEVVQTWQKVFRGDTVLNLCLREIQQQRAAQKLIYTFNQVKPHSIPYTLRFLEVFLIFCHSANQWLTIEKYLTGEFRKYNNNNGDEITPTTLLEETLLSFSHWSYEYTRGELLVLDLQGVGENLTDPSVIKPEDKQSRGMVFGPANLGEDALTNYVSKHRCNSCCRKLKLLVHSFCLTLFLRTVAAVAAETLDVFLSINVLIDHKSYLKRNDYSPGRINLGFEIGEADTDKDLVEYNTRL

Target ORF information:

RefSeq Version	XM_031890885.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis transient receptor potential cation channel, subfamily M, member 6 (trpm6), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031890885.1

ORF Insert Sequence:

ATGTCTGCTC AAGACCAGCT GAGCTTGGCG CTAACCTGGA ACAGGGTTGA CATTGCCAAA 
AAACACATAC TTATATATGG ACAACATTGG AAGGTGGGGT CTTTGGAACA AGCAATGCAA 
GATGCACTTG TAATGGATCG GGTGGATTTT GTAAAGTTGC TAATTGAATA TGGTGTGAAT 
ATGCATCGCT TCTTGACCAT TGGTCATTTG GAAGAATTAT ACAATACTAA ACAAGGCCCC 
ACAAATCTGC TCCTACACCA TTTAGTCAGA GATGTAAAAC AGAGCAATCC TTCTCCAGAT 
TACAGAATTT CCCTTATAGA TGTGGGACTT GTAATCGAGT ACTTGATTGG TGGAGCCTAC 
CGAAGCAGTT ATACAAGGAA AAGTTTCAGA GTCCTGTACA ACAGTCTCTT CAGGAAGCAT 
AAGAAATCTG TGCCCAGTTC CTCCAGCAGC CTTGCACAAA GTTTATCTCA TCTTTCCATA 
AACCAGAGCA GCCAGATGAG CAGCAAGCTT GAATCGCCAG AGAGTACCTT GCATTCCCAA 
TTTTTCCGAA CAGCCCAACC TTGTAGACGC AAGGACAAGA CAACATGCCA TCAAAAGTCA 
AAAAAGAAAG ATGAAGTAAC TTGCACAGAA GATAATGAAC CAGTTGGCTT TATCTACCCT 
TACAATGACC TCTTGGTTTG GGCTGTACTG ATGAAAAGAC AGAAGATGGC TATGTTTTTT 
TGGCAACATG GAGAGGAGGC CATGGTCAAA GCAGTAATTG CCTGTAAGCT ATATAGGGCC 
ATGGCACATG AAGCTAAGCA GAGCACCATG GTGGATGACA CTTCAGAAGA ATTAAAGAAA 
TATTCAAAAG AATTTGGACA GCTTGCTTTG GACATACTTG AAAATGCTTT CAAAATGAAT 
GAACAAATGG CCATGAAACT TTTAACATAC CAGTTAAAGA ACTGGAGTAA TTCAACGTGC 
CTGAAATTAG CGGTTTCATC AACATTGCGG CCCTTTGTAT CCCATACATG CACACAGATG 
TTACTGACAG ACATGTGGAT GGGTCGCCTG AAGATGCGGA AACACTCCTG GCTTAAAGTC 
ATTCTAGGAA TATTGCTGCC ACCCTTTGTC TTGATGTTGG AATTTAAGAC CAAGGCAGAA 
ATGTCCCATG TACCCCAGTC ACTAGAGTTC CATCAGTTCT CACGGCTGTA CGTTGACCCT 
CCATCACTGA GCACCAAAGA GGAACATATT ACAGATTTTG ATGCTGAAAG GGGATCAAAG 
CAGCCAGGAG AAGATCAAAA TCAGAAGATC AGTAATGGTC TAGGAATCCT TCCGATAACC 
AGGAAAATTT GTGAATTTTA TAGCGCTCCC ATTGTCAAGT TCTGGTTCCA CACTATGGTG 
TACTTGGCAT TCCTCATGCT TTTCACATAC ACGGTGCTGG TAAAGATGGA CCCACAGCCC 
AGTGTCCAAG AGTGGTGTGT AATAATTTAT ATATTCACTA CGGCAGTTGA AAAAGTCAGG 
GAGGTATTTG TGTCAGAACC TGGAAAGTTC AGTCAAAAAG TAAAAGTTTG GATCAATGAG 
TACTGGAATC TTACAGATTC TATTGCAATT TTCTTGTTTT TTATTGGTTT CGGCTTGCGC 
TGGCCCGACC CTCCGTTTCA AACAGCAGGG CGCATAATAT ATTGCCTGGA CATAATATTT 
TGGTATGTGC GACTTTTAGA CTTCTTTGCA GTGAACCAGC AAGCAGGCCC TTACATTACA 
ATGCTTGGAA AAATGACATC CAACATGTTT GGTATTGTGA TTATAATGGC AATTGTTCTG 
TTGGCATTTG GGGTGTCCCG GAAAGCAATT CTGTCTCCCG ATCACCCTCC TTCTTGGACT 
CTTGCTCGTG ATATAGTGTT TCAGCCATAC TGGATGATGT TTGGGGAAGT ATATGCTGGG 
GAAATTGATG CCTGTGAAGA AGATCCAGAT TGCCCTCCTG GTTCCTTCAT CACCCCATTC 
CTTCAAGCTG TGTATCTCTT TGTGCAGTAT ATCATCATGG TCAACCTGCT TATTTCTCTG 
TTTAATAATG TCTACAGGGT ATTAAACTCT GTCTCTCACA AACTTTGGAA ATACAATCGA 
TATCGATATA TAATGACATA CTACGAGAAG CCATGGCTAC CTCCACCCCT GATATTCCTT 
AGTCATATAT ATATACTTAT CAATCGCCTT TGTCATCACC GATCTCACAG TGAGTTTGAC 
CCAGAGGAAA CTGACATGGG ACTGAAGCTG TACCTTAGTG ATAATGAATT GAGGAAACTC 
CATGATTTTG AGGAAAAATG TGTGGAAACA TACTTCCATG AGAAAAATGA AAGCCTGCAC 
TCCAGCAAAA ATGAAAGAAT CCGTGTAACA ACAGAGAGAA CTGAAGAGAT GTTTGCCCAA 
CTGAAAGAAG TTAATGATAA AGTTTTCTAC ATCAAAGACT CCTTGAGCTC CCTGGACAGC 
CAATTAGGGC ATTTGCAGGA TCTCTCCGCA TTGACTGTGG ATACTTTAAA AATCATTTCA 
GCTGTGGATT CTTTGCAAGT TGAAGAAGCC TTTATAAAAG ACAAGAAAGG ACTCACCTGT 
AAAAAGCTCC CACACAGTTG GAGCAACGTT CCATATTCTC ATCACCAGAG AGAGTATAAT 
TACTACAGTA TGCCACCTTC ACTTCTCCGG ACCTTGGCCA GGAGTCAGTG CCCATCACTG 
TATCAATGTC CACCACAGCA ACTGACTGCA CAGTTAGAAA TCCATAACTC AGAAGAACCC 
AAGATAGAGG TTGATATTGT TACTATGGCA GTGCCATTGG ATAGTAAATC AGCTCAGCAT 
TACGGCAATT ACTTGCTTGT ACCTTCAGTG GGGCCCCCTG CACCAACTGA TTCTGCCTTT 
AAAGAGACAT GTTCAGATAT GCTCAGTGAA GGGGAAGCAA TATCTGTACA AGCTACAACC 
AAAGAGAGGA TATGTTCCAT TCATTCTGAC CAGTGTCTCT GTAGCAAACA AAAGCATGTG 
AAGGAAAGTA GTCGCAAGAC CATGAAAGAG GATTTAAAGC CTCTTGTGAC AAATACTGAT 
GAAAGCCAAA ATGAAGAAAG CCATGAATGC TCTTGCGATC AGTTTACTGC TCATGTCACT 
GTTGAGACCT CAATTACGTG CAACATTATT GACCCCGGAG GTGGTTATGT AAACTTGGCA 
TTTTCAGAAG AAGGAGAAAC TGCCCTGTTT AGTGGAGGGA GTAGCAAGAC CCTTTGCTCA 
CGTCCCACCC AAAGTTCAAA ATCTAATGTG TTTCCAAATA AGTCTGATGG ATCTAGAGCC 
AATGATAGGC CTGAGTTTGG TTCAGGTTAC AAACAGAAAA CTTCATTCCT GAAAAATTCA 
CTTTATAGGA ATAACTCACC AAGAGGCAGT TCTAAGAGGC ATAAAGAGAA GAAAGATGGA 
AAGATATGTA CAATCAAAGA TTTTGCCAAG CCTCCTGAGA GCTCTAAGGT TACATGGGCC 
AAAGAGAAAC TGCGTAGCAC ATTCAGAAAG TCTGTTAAAG ACAAGCCAAG GCCAACTCAA 
CTTCAAGTTC CAGTGATAAC TGTGGATGGT TGCTCTCAGA GTACGCAGAT GAGTTCCGAA 
CCTGCAGATT GTGGCAGCTG GAAATCTGAA GATGAACCCA GTAAGAACAG GCTTACAGTA 
TCAAGTTTCA ATCAGCTAAG CTTGGATCAC ATGACTTACA TGCGACAGCA ACTAAAGACG 
CAAGAATTTG AAAGACACCT AGTTCAGACT AGTGAATATC TAAGGCAGTC CAGAGAGGAT 
TTGCATAACA CCACCCAACT GAGAAGGAGC TCATTATTCC GAAATTCCGA TGAAGTAAAG 
AAAATGGCAG CTTCCCTAAA AGCCTCTCAG GATTTAAGTA ATCATTATTC AGCGGTGGAA 
AGGAATAATT TAATGAGGCT TGCACAGAGC ATACCTTTCA CCCCCCTGCA CCTTTTTGCT 
GGAGAAGAAG TCACTGTTTA CCAACTAGAG GAAAGTTCTC CAGCAATTCT AGATAAAAGT 
ATGTCTTCCT GGTCACAGCG TGGAATGGCA GCAATGCTTC AGGTTCTGTC CCCAGAGGAA 
ATGGATGGAG GTCTCCGCAG GGCGTTAAAA GTTGTGTGCA CTTGGTCTGA GCATGATGTA 
CTGACGCCTG GTGAGGTGTT TATAGTCAAA TCTTTTCTTC CGGAGGTAGT ACAGACTTGG 
CAGAAGGTTT TCCGTGGTGA TACTGTACTG AATCTGTGTC TCAGGGAAAT TCAGCAACAA 
AGAGCAGCTC AAAAGTTGAT CTACACCTTC AATCAAGTGA AACCACACAG TATTCCTTAT 
ACTCTAAGGT TTCTAGAAGT ATTTCTTATA TTCTGCCATT CAGCTAACCA ATGGCTTACT 
ATAGAGAAGT ACCTGACTGG AGAATTCCGC AAGTACAATA ACAACAATGG AGATGAAATT 
ACCCCCACCA CTTTACTTGA GGAGACACTG CTGTCTTTTT CTCATTGGAG TTATGAATAT 
ACAAGGGGAG AGCTGCTTGT TTTGGATTTA CAAGGCGTTG GGGAAAATCT TACAGACCCA 
TCTGTTATTA AACCAGAAGA CAAACAATCA AGAGGAATGG TATTTGGACC AGCTAATTTA 
GGAGAAGATG CTCTGACTAA TTATGTTTCA AAGCATCGCT GCAATTCCTG CTGCAGAAAG 
CTTAAACTCC TTGTGCACTC ATTCTGTCTC ACCTTGTTTT TGAGAACAGT AGCAGCTGTA 
GCAGCCGAAA CGTTAGATGT ATTCCTATCA ATAAATGTTT TGATTGACCA TAAGTCCTAT 
TTGAAAAGAA ATGACTACTC ACCTGGGAGG ATCAATCTAG GGTTTGAAAT TGGAGAAGCA 
GACACAGATA AAGACCTTGT GGAATATAAT ACAAGACTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpm6 ( XM_002940033.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002940079.2 Xenopus tropicalis transient receptor potential cation channel, subfamily M, member 6 (trpm6), transcript variant X2, mRNA.

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CloneID	OXa05105
Clone ID Related Accession (Same CDS sequence)	XM_002940033.4 , XM_002940033.5
Accession Version	XM_002940033.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6006bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transient receptor potential cation channel subfamily M member 6 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002940033.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTCC AGCTAAAGTC TCAGAAATCA TGGATTGAAG GGCTCTTCAA TAAAAGAGAA 
TGTGCAAAAA TCATACCGTC ATCAAAAGAG CCACACAGAT GCCACGGTGG ATGTCAGGTT 
TGCCAGAATT TAATAAGGTG TTGTTGTGGA CGGTTAATTG GAGACCACCC TGGGTTAGAT 
TATGGATGGC CAATTAATCC TTCTCCCCAA GAAAGAGAAG ACGAAGAGTG GAGTATTCTA 
AATCATACAA AACCAAGTCC CACTGATGCA TTTGGCACAA TAAATTTTCA AGATGGAGAT 
CACACCTATC ATGCAAAGTA CATAAGAATA TCCTATGATA CCACATTGGA ACTGTTAATG 
CATCTGATGA TTAAAGAATG GCAAATGGAA CTGCCCAAGC TTGTGATCTC TGTCCATGGG 
GGCATACAGA ATTTTAAACT TCCGTCCAAA ATAAAGCAAG TTTTTGGCAA AGGATTGGTG 
AAAGCTGCTG AGACAACAGG AGCATGGATC CTTACAGAGG GCATTAATAC TGGGGCCTCC 
AAACATGTGG GTGATGCTTT AAAAGCACAC GCTTCTCAAC ACCTGCGAAA AATCTGTGCA 
GTTGGCATAC CACCATGGGG AGCTATTGAG AATCAGCAGG ATCTCATTGG CAAAGACGTT 
GTGTGCCTGT ATCAGACACT GATAAATCCA ATGAGCAAAC TGACAAGTCT CAATAGCATG 
CACTCCCATT TTATAATGGT GGATGATGGC ACAGTTGGAA AGTATGGTAA TGAAATGAAA 
TTAAGGAGAA ACTTGGAAGA CTTCATTTCC CTTCAGAAGA TCCATACCCG GATGGGGCAA 
GGAGTGCCTG TGGTAGGGCT TGTTATAGAA GGTGGTCCAA ATGTCATCTT GATGGTATGG 
GAATATGTGA GGAGCACTCC ACCAGTACCA GTTGTAGTGT GTGAAGGAAC AGGCAGAGCA 
GCTGATATAT TGGCATTTAC TCACAAGCAC ACAACGGACA CAGAGCAGAT TAGTCCACAG 
CTGAAAGAAG AAATCTTAGA GATGATTCAG AAGACATTTA ATCTTGGCCA CAGACAATCA 
AACCATGTTC TTTATATTCT AATGGAGTGT ATGGAGCGCC GTGCGTCTAT TACTATATTT 
GATGCAGAAT CTGAAGAACA GCAAGATATT GATTTGGCTA TATTGACAGC TCTGCTAAAA 
GGGACTAATA TGTCTGCTCA AGACCAGCTG AGCTTGGCGC TAACCTGGAA CAGGGTTGAC 
ATTGCCAAAA AACACATACT TATATATGGA CAACATTGGA AGGTGGGGTC TTTGGAACAA 
GCAATGCAAG ATGCACTTGT AATGGATCGG GTGGATTTTG TAAAGTTGCT AATTGAATAT 
GGTGTGAATA TGCATCGCTT CTTGACCATT GGTCATTTGG AAGAATTATA CAATACTAAA 
CAAGGCCCCA CAAATCTGCT CCTACACCAT TTAGTCAGAG ATGTAAAACA GAGCAATCCT 
TCTCCAGATT ACAGAATTTC CCTTATAGAT GTGGGACTTG TAATCGAGTA CTTGATTGGT 
GGAGCCTACC GAAGCAGTTA TACAAGGAAA AGTTTCAGAG TCCTGTACAA CAGTCTCTTC 
AGGAAGCATA AGAAATCTGT GCCCAGTTCC TCCAGCAGCC TTGCACAAAG TTTATCTCAT 
CTTTCCATAA ACCAGAGCAG CCAGATGAGC AGCAAGCTTG AATCGCCAGA GAGTACCTTG 
CATTCCCAAT TTTTCCGAAC AGCCCAACCT TGTAGACGCA AGGACAAGAC AACATGCCAT 
CAAAAGTCAA AAAAGAAAGA TGAAGTAACT TGCACAGAAG ATAATGAACC AGTTGGCTTT 
ATCTACCCTT ACAATGACCT CTTGGTTTGG GCTGTACTGA TGAAAAGACA GAAGATGGCT 
ATGTTTTTTT GGCAACATGG AGAGGAGGCC ATGGTCAAAG CAGTAATTGC CTGTAAGCTA 
TATAGGGCCA TGGCACATGA AGCTAAGCAG AGCACCATGG TGGATGACAC TTCAGAAGAA 
TTAAAGAAAT ATTCAAAAGA ATTTGGACAG CTTGCTTTGG ACATACTTGA AAATGCTTTC 
AAAATGAATG AACAAATGGC CATGAAACTT TTAACATACC AGTTAAAGAA CTGGAGTAAT 
TCAACGTGCC TGAAATTAGC GGTTTCATCA ACATTGCGGC CCTTTGTATC CCATACATGC 
ACACAGATGT TACTGACAGA CATGTGGATG GGTCGCCTGA AGATGCGGAA ACACTCCTGG 
CTTAAAGTCA TTCTAGGAAT ATTGCTGCCA CCCTTTGTCT TGATGTTGGA ATTTAAGACC 
AAGGCAGAAA TGTCCCATGT ACCCCAGTCA CTAGAGTTCC ATCAGTTCTC ACGGCTGTAC 
GTTGACCCTC CATCACTGAG CACCAAAGAG GAACATATTA CAGATTTTGA TGCTGAAAGG 
GGATCAAAGC AGCCAGGAGA AGATCAAAAT CAGAAGATCA GTAATGGTCT AGGAATCCTT 
CCGATAACCA GGAAAATTTG TGAATTTTAT AGCGCTCCCA TTGTCAAGTT CTGGTTCCAC 
ACTATGGTGT ACTTGGCATT CCTCATGCTT TTCACATACA CGGTGCTGGT AAAGATGGAC 
CCACAGCCCA GTGTCCAAGA GTGGTGTGTA ATAATTTATA TATTCACTAC GGCAGTTGAA 
AAAGTCAGGG AGGTATTTGT GTCAGAACCT GGAAAGTTCA GTCAAAAAGT AAAAGTTTGG 
ATCAATGAGT ACTGGAATCT TACAGATTCT ATTGCAATTT TCTTGTTTTT TATTGGTTTC 
GGCTTGCGCT GGCCCGACCC TCCGTTTCAA ACAGCAGGGC GCATAATATA TTGCCTGGAC 
ATAATATTTT GGTATGTGCG ACTTTTAGAC TTCTTTGCAG TGAACCAGCA AGCAGGCCCT 
TACATTACAA TGCTTGGAAA AATGACATCC AACATGTTTG GTATTGTGAT TATAATGGCA 
ATTGTTCTGT TGGCATTTGG GGTGTCCCGG AAAGCAATTC TGTCTCCCGA TCACCCTCCT 
TCTTGGACTC TTGCTCGTGA TATAGTGTTT CAGCCATACT GGATGATGTT TGGGGAAGTA 
TATGCTGGGG AAATTGATGC CTGTGAAGAA GATCCAGATT GCCCTCCTGG TTCCTTCATC 
ACCCCATTCC TTCAAGCTGT GTATCTCTTT GTGCAGTATA TCATCATGGT CAACCTGCTT 
ATTTCTCTGT TTAATAATGT CTACAGGGTA TTAAACTCTG TCTCTCACAA ACTTTGGAAA 
TACAATCGAT ATCGATATAT AATGACATAC TACGAGAAGC CATGGCTACC TCCACCCCTG 
ATATTCCTTA GTCATATATA TATACTTATC AATCGCCTTT GTCATCACCG ATCTCACAGT 
GAGTTTGACC CAGAGGAAAC TGACATGGGA CTGAAGCTGT ACCTTAGTGA TAATGAATTG 
AGGAAACTCC ATGATTTTGA GGAAAAATGT GTGGAAACAT ACTTCCATGA GAAAAATGAA 
AGCCTGCACT CCAGCAAAAA TGAAAGAATC CGTGTAACAA CAGAGAGAAC TGAAGAGATG 
TTTGCCCAAC TGAAAGAAGT TAATGATAAA GTTTTCTACA TCAAAGACTC CTTGAGCTCC 
CTGGACAGCC AATTAGGGCA TTTGCAGGAT CTCTCCGCAT TGACTGTGGA TACTTTAAAA 
ATCATTTCAG CTGTGGATTC TTTGCAAGTT GAAGAAGCCT TTATAAAAGA CAAGAAAGGA 
CTCACCTGTA AAAAGCTCCC ACACAGTTGG AGCAACGTTC CATATTCTCA TCACCAGAGA 
GAGTATAATT ACTACAGTAT GCCACCTTCA CTTCTCCGGA CCTTGGCCAG GAGTCAGTGC 
CCATCACTGT ATCAATGTCC ACCACAGCAA CTGACTGCAC AGTTAGAAAT CCATAACTCA 
GAAGAACCCA AGATAGAGGT TGATATTGTT ACTATGGCAG TGCCATTGGA TAGTAAATCA 
GCTCAGCATT ACGGCAATTA CTTGCTTGTA CCTTCAGTGG GGCCCCCTGC ACCAACTGAT 
TCTGCCTTTA AAGAGACATG TTCAGATATG CTCAGTGAAG GGGAAGCAAT ATCTGTACAA 
GCTACAACCA AAGAGAGGAT ATGTTCCATT CATTCTGACC AGTGTCTCTG TAGCAAACAA 
AAGCATGTGA AGGAAAGTAG TCGCAAGACC ATGAAAGAGG ATTTAAAGCC TCTTGTGACA 
AATACTGATG AAAGCCAAAA TGAAGAAAGC CATGAATGCT CTTGCGATCA GTTTACTGCT 
CATGTCACTG TTGAGACCTC AATTACGTGC AACATTATTG ACCCCGGAGG TGGTTATGTA 
AACTTGGCAT TTTCAGAAGA AGGAGAAACT GCCCTGTTTA GTGGAGGGAG TAGCAAGACC 
CTTTGCTCAC GTCCCACCCA AAGTTCAAAA TCTAATGTGT TTCCAAATAA GTCTGATGGA 
TCTAGAGCCA ATGATAGGCC TGAGTTTGGT TCAGGTTACA AACAGAAAAC TTCATTCCTG 
AAAAATTCAC TTTATAGGAA TAACTCACCA AGAGGCAGTT CTAAGAGGCA TAAAGAGAAG 
AAAGATGGAA AGATATGTAC AATCAAAGAT TTTGCCAAGC CTCCTGAGAG CTCTAAGGTT 
ACATGGGCCA AAGAGAAACT GCGTAGCACA TTCAGAAAGT CTGTTAAAGA CAAGCCAAGG 
CCAACTCAAC TTCAAGTTCC AGTGATAACT GTGGATGGTT GCTCTCAGAG TACGCAGATG 
AGTTCCGAAC CTGCAGATTG TGGCAGCTGG AAATCTGAAG ATGAACCCAG TAAGAACAGG 
CTTACAGTAT CAAGTTTCAA TCAGCTAAGC TTGGATCACA TGACTTACAT GCGACAGCAA 
CTAAAGACGC AAGAATTTGA AAGACACCTA GTTCAGACTA GTGAATATCT AAGGCAGTCC 
AGAGAGGATT TGCATAACAC CACCCAACTG AGAAGGAGCT CATTATTCCG AAATTCCGAT 
GAAGTAAAGA AAATGGCAGC TTCCCTAAAA GCCTCTCAGG ATTTAAGTAA TCATTATTCA 
GCGGTGGAAA GGAATAATTT AATGAGGCTT GCACAGAGCA TACCTTTCAC CCCCCTGCAC 
CTTTTTGCTG GAGAAGAAGT CACTGTTTAC CAACTAGAGG AAAGTTCTCC AGCAATTCTA 
GATAAAAGTA TGTCTTCCTG GTCACAGCGT GGAATGGCAG CAATGCTTCA GGTTCTGTCC 
CCAGAGGAAA TGGATGGAGG TCTCCGCAGG GCGTTAAAAG TTGTGTGCAC TTGGTCTGAG 
CATGATGTAC TGACGCCTGG TGAGGTGTTT ATAGTCAAAT CTTTTCTTCC GGAGGTAGTA 
CAGACTTGGC AGAAGGTTTT CCGTGGTGAT ACTGTACTGA ATCTGTGTCT CAGGGAAATT 
CAGCAACAAA GAGCAGCTCA AAAGTTGATC TACACCTTCA ATCAAGTGAA ACCACACAGT 
ATTCCTTATA CTCTAAGGTT TCTAGAAGTA TTTCTTATAT TCTGCCATTC AGCTAACCAA 
TGGCTTACTA TAGAGAAGTA CCTGACTGGA GAATTCCGCA AGTACAATAA CAACAATGGA 
GATGAAATTA CCCCCACCAC TTTACTTGAG GAGACACTGC TGTCTTTTTC TCATTGGAGT 
TATGAATATA CAAGGGGAGA GCTGCTTGTT TTGGATTTAC AAGGCGTTGG GGAAAATCTT 
ACAGACCCAT CTGTTATTAA ACCAGAAGAC AAACAATCAA GAGGAATGGT ATTTGGACCA 
GCTAATTTAG GAGAAGATGC TCTGACTAAT TATGTTTCAA AGCATCGCTG CAATTCCTGC 
TGCAGAAAGC TTAAACTCCT TGATTTGAAA AGAAATGACT ACTCACCTGG GAGGATCAAT 
CTAGGGTTTG AAATTGGAGA AGCAGACACA GATAAAGACC TTGTGGAATA TAATACAAGA 
CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002940079.2
CDS	290..6295
Translation	MKVQLKSQKSWIEGLFNKRECAKIIPSSKEPHRCHGGCQVCQNLIRCCCGRLIGDHPGLDYGWPINPSPQEREDEEWSILNHTKPSPTDAFGTINFQDGDHTYHAKYIRISYDTTLELLMHLMIKEWQMELPKLVISVHGGIQNFKLPSKIKQVFGKGLVKAAETTGAWILTEGINTGASKHVGDALKAHASQHLRKICAVGIPPWGAIENQQDLIGKDVVCLYQTLINPMSKLTSLNSMHSHFIMVDDGTVGKYGNEMKLRRNLEDFISLQKIHTRMGQGVPVVGLVIEGGPNVILMVWEYVRSTPPVPVVVCEGTGRAADILAFTHKHTTDTEQISPQLKEEILEMIQKTFNLGHRQSNHVLYILMECMERRASITIFDAESEEQQDIDLAILTALLKGTNMSAQDQLSLALTWNRVDIAKKHILIYGQHWKVGSLEQAMQDALVMDRVDFVKLLIEYGVNMHRFLTIGHLEELYNTKQGPTNLLLHHLVRDVKQSNPSPDYRISLIDVGLVIEYLIGGAYRSSYTRKSFRVLYNSLFRKHKKSVPSSSSSLAQSLSHLSINQSSQMSSKLESPESTLHSQFFRTAQPCRRKDKTTCHQKSKKKDEVTCTEDNEPVGFIYPYNDLLVWAVLMKRQKMAMFFWQHGEEAMVKAVIACKLYRAMAHEAKQSTMVDDTSEELKKYSKEFGQLALDILENAFKMNEQMAMKLLTYQLKNWSNSTCLKLAVSSTLRPFVSHTCTQMLLTDMWMGRLKMRKHSWLKVILGILLPPFVLMLEFKTKAEMSHVPQSLEFHQFSRLYVDPPSLSTKEEHITDFDAERGSKQPGEDQNQKISNGLGILPITRKICEFYSAPIVKFWFHTMVYLAFLMLFTYTVLVKMDPQPSVQEWCVIIYIFTTAVEKVREVFVSEPGKFSQKVKVWINEYWNLTDSIAIFLFFIGFGLRWPDPPFQTAGRIIYCLDIIFWYVRLLDFFAVNQQAGPYITMLGKMTSNMFGIVIIMAIVLLAFGVSRKAILSPDHPPSWTLARDIVFQPYWMMFGEVYAGEIDACEEDPDCPPGSFITPFLQAVYLFVQYIIMVNLLISLFNNVYRVLNSVSHKLWKYNRYRYIMTYYEKPWLPPPLIFLSHIYILINRLCHHRSHSEFDPEETDMGLKLYLSDNELRKLHDFEEKCVETYFHEKNESLHSSKNERIRVTTERTEEMFAQLKEVNDKVFYIKDSLSSLDSQLGHLQDLSALTVDTLKIISAVDSLQVEEAFIKDKKGLTCKKLPHSWSNVPYSHHQREYNYYSMPPSLLRTLARSQCPSLYQCPPQQLTAQLEIHNSEEPKIEVDIVTMAVPLDSKSAQHYGNYLLVPSVGPPAPTDSAFKETCSDMLSEGEAISVQATTKERICSIHSDQCLCSKQKHVKESSRKTMKEDLKPLVTNTDESQNEESHECSCDQFTAHVTVETSITCNIIDPGGGYVNLAFSEEGETALFSGGSSKTLCSRPTQSSKSNVFPNKSDGSRANDRPEFGSGYKQKTSFLKNSLYRNNSPRGSSKRHKEKKDGKICTIKDFAKPPESSKVTWAKEKLRSTFRKSVKDKPRPTQLQVPVITVDGCSQSTQMSSEPADCGSWKSEDEPSKNRLTVSSFNQLSLDHMTYMRQQLKTQEFERHLVQTSEYLRQSREDLHNTTQLRRSSLFRNSDEVKKMAASLKASQDLSNHYSAVERNNLMRLAQSIPFTPLHLFAGEEVTVYQLEESSPAILDKSMSSWSQRGMAAMLQVLSPEEMDGGLRRALKVVCTWSEHDVLTPGEVFIVKSFLPEVVQTWQKVFRGDTVLNLCLREIQQQRAAQKLIYTFNQVKPHSIPYTLRFLEVFLIFCHSANQWLTIEKYLTGEFRKYNNNNGDEITPTTLLEETLLSFSHWSYEYTRGELLVLDLQGVGENLTDPSVIKPEDKQSRGMVFGPANLGEDALTNYVSKHRCNSCCRKLKLLDLKRNDYSPGRINLGFEIGEADTDKDLVEYNTRL

Target ORF information:

RefSeq Version	XM_002940033.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis transient receptor potential cation channel, subfamily M, member 6 (trpm6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002940033.5

ORF Insert Sequence:

ATGAAAGTCC AGCTAAAGTC TCAGAAATCA TGGATTGAAG GGCTCTTCAA TAAAAGAGAA 
TGTGCAAAAA TCATACCGTC ATCAAAAGAG CCACACAGAT GCCACGGTGG ATGTCAGGTT 
TGCCAGAATT TAATAAGGTG TTGTTGTGGA CGGTTAATTG GAGACCACCC TGGGTTAGAT 
TATGGATGGC CAATTAATCC TTCTCCCCAA GAAAGAGAAG ACGAAGAGTG GAGTATTCTA 
AATCATACAA AACCAAGTCC CACTGATGCA TTTGGCACAA TAAATTTTCA AGATGGAGAT 
CACACCTATC ATGCAAAGTA CATAAGAATA TCCTATGATA CCACATTGGA ACTGTTAATG 
CATCTGATGA TTAAAGAATG GCAAATGGAA CTGCCCAAGC TTGTGATCTC TGTCCATGGG 
GGCATACAGA ATTTTAAACT TCCGTCCAAA ATAAAGCAAG TTTTTGGCAA AGGATTGGTG 
AAAGCTGCTG AGACAACAGG AGCATGGATC CTTACAGAGG GCATTAATAC TGGGGCCTCC 
AAACATGTGG GTGATGCTTT AAAAGCACAC GCTTCTCAAC ACCTGCGAAA AATCTGTGCA 
GTTGGCATAC CACCATGGGG AGCTATTGAG AATCAGCAGG ATCTCATTGG CAAAGACGTT 
GTGTGCCTGT ATCAGACACT GATAAATCCA ATGAGCAAAC TGACAAGTCT CAATAGCATG 
CACTCCCATT TTATAATGGT GGATGATGGC ACAGTTGGAA AGTATGGTAA TGAAATGAAA 
TTAAGGAGAA ACTTGGAAGA CTTCATTTCC CTTCAGAAGA TCCATACCCG GATGGGGCAA 
GGAGTGCCTG TGGTAGGGCT TGTTATAGAA GGTGGTCCAA ATGTCATCTT GATGGTATGG 
GAATATGTGA GGAGCACTCC ACCAGTACCA GTTGTAGTGT GTGAAGGAAC AGGCAGAGCA 
GCTGATATAT TGGCATTTAC TCACAAGCAC ACAACGGACA CAGAGCAGAT TAGTCCACAG 
CTGAAAGAAG AAATCTTAGA GATGATTCAG AAGACATTTA ATCTTGGCCA CAGACAATCA 
AACCATGTTC TTTATATTCT AATGGAGTGT ATGGAGCGCC GTGCGTCTAT TACTATATTT 
GATGCAGAAT CTGAAGAACA GCAAGATATT GATTTGGCTA TATTGACAGC TCTGCTAAAA 
GGGACTAATA TGTCTGCTCA AGACCAGCTG AGCTTGGCGC TAACCTGGAA CAGGGTTGAC 
ATTGCCAAAA AACACATACT TATATATGGA CAACATTGGA AGGTGGGGTC TTTGGAACAA 
GCAATGCAAG ATGCACTTGT AATGGATCGG GTGGATTTTG TAAAGTTGCT AATTGAATAT 
GGTGTGAATA TGCATCGCTT CTTGACCATT GGTCATTTGG AAGAATTATA CAATACTAAA 
CAAGGCCCCA CAAATCTGCT CCTACACCAT TTAGTCAGAG ATGTAAAACA GAGCAATCCT 
TCTCCAGATT ACAGAATTTC CCTTATAGAT GTGGGACTTG TAATCGAGTA CTTGATTGGT 
GGAGCCTACC GAAGCAGTTA TACAAGGAAA AGTTTCAGAG TCCTGTACAA CAGTCTCTTC 
AGGAAGCATA AGAAATCTGT GCCCAGTTCC TCCAGCAGCC TTGCACAAAG TTTATCTCAT 
CTTTCCATAA ACCAGAGCAG CCAGATGAGC AGCAAGCTTG AATCGCCAGA GAGTACCTTG 
CATTCCCAAT TTTTCCGAAC AGCCCAACCT TGTAGACGCA AGGACAAGAC AACATGCCAT 
CAAAAGTCAA AAAAGAAAGA TGAAGTAACT TGCACAGAAG ATAATGAACC AGTTGGCTTT 
ATCTACCCTT ACAATGACCT CTTGGTTTGG GCTGTACTGA TGAAAAGACA GAAGATGGCT 
ATGTTTTTTT GGCAACATGG AGAGGAGGCC ATGGTCAAAG CAGTAATTGC CTGTAAGCTA 
TATAGGGCCA TGGCACATGA AGCTAAGCAG AGCACCATGG TGGATGACAC TTCAGAAGAA 
TTAAAGAAAT ATTCAAAAGA ATTTGGACAG CTTGCTTTGG ACATACTTGA AAATGCTTTC 
AAAATGAATG AACAAATGGC CATGAAACTT TTAACATACC AGTTAAAGAA CTGGAGTAAT 
TCAACGTGCC TGAAATTAGC GGTTTCATCA ACATTGCGGC CCTTTGTATC CCATACATGC 
ACACAGATGT TACTGACAGA CATGTGGATG GGTCGCCTGA AGATGCGGAA ACACTCCTGG 
CTTAAAGTCA TTCTAGGAAT ATTGCTGCCA CCCTTTGTCT TGATGTTGGA ATTTAAGACC 
AAGGCAGAAA TGTCCCATGT ACCCCAGTCA CTAGAGTTCC ATCAGTTCTC ACGGCTGTAC 
GTTGACCCTC CATCACTGAG CACCAAAGAG GAACATATTA CAGATTTTGA TGCTGAAAGG 
GGATCAAAGC AGCCAGGAGA AGATCAAAAT CAGAAGATCA GTAATGGTCT AGGAATCCTT 
CCGATAACCA GGAAAATTTG TGAATTTTAT AGCGCTCCCA TTGTCAAGTT CTGGTTCCAC 
ACTATGGTGT ACTTGGCATT CCTCATGCTT TTCACATACA CGGTGCTGGT AAAGATGGAC 
CCACAGCCCA GTGTCCAAGA GTGGTGTGTA ATAATTTATA TATTCACTAC GGCAGTTGAA 
AAAGTCAGGG AGGTATTTGT GTCAGAACCT GGAAAGTTCA GTCAAAAAGT AAAAGTTTGG 
ATCAATGAGT ACTGGAATCT TACAGATTCT ATTGCAATTT TCTTGTTTTT TATTGGTTTC 
GGCTTGCGCT GGCCCGACCC TCCGTTTCAA ACAGCAGGGC GCATAATATA TTGCCTGGAC 
ATAATATTTT GGTATGTGCG ACTTTTAGAC TTCTTTGCAG TGAACCAGCA AGCAGGCCCT 
TACATTACAA TGCTTGGAAA AATGACATCC AACATGTTTG GTATTGTGAT TATAATGGCA 
ATTGTTCTGT TGGCATTTGG GGTGTCCCGG AAAGCAATTC TGTCTCCCGA TCACCCTCCT 
TCTTGGACTC TTGCTCGTGA TATAGTGTTT CAGCCATACT GGATGATGTT TGGGGAAGTA 
TATGCTGGGG AAATTGATGC CTGTGAAGAA GATCCAGATT GCCCTCCTGG TTCCTTCATC 
ACCCCATTCC TTCAAGCTGT GTATCTCTTT GTGCAGTATA TCATCATGGT CAACCTGCTT 
ATTTCTCTGT TTAATAATGT CTACAGGGTA TTAAACTCTG TCTCTCACAA ACTTTGGAAA 
TACAATCGAT ATCGATATAT AATGACATAC TACGAGAAGC CATGGCTACC TCCACCCCTG 
ATATTCCTTA GTCATATATA TATACTTATC AATCGCCTTT GTCATCACCG ATCTCACAGT 
GAGTTTGACC CAGAGGAAAC TGACATGGGA CTGAAGCTGT ACCTTAGTGA TAATGAATTG 
AGGAAACTCC ATGATTTTGA GGAAAAATGT GTGGAAACAT ACTTCCATGA GAAAAATGAA 
AGCCTGCACT CCAGCAAAAA TGAAAGAATC CGTGTAACAA CAGAGAGAAC TGAAGAGATG 
TTTGCCCAAC TGAAAGAAGT TAATGATAAA GTTTTCTACA TCAAAGACTC CTTGAGCTCC 
CTGGACAGCC AATTAGGGCA TTTGCAGGAT CTCTCCGCAT TGACTGTGGA TACTTTAAAA 
ATCATTTCAG CTGTGGATTC TTTGCAAGTT GAAGAAGCCT TTATAAAAGA CAAGAAAGGA 
CTCACCTGTA AAAAGCTCCC ACACAGTTGG AGCAACGTTC CATATTCTCA TCACCAGAGA 
GAGTATAATT ACTACAGTAT GCCACCTTCA CTTCTCCGGA CCTTGGCCAG GAGTCAGTGC 
CCATCACTGT ATCAATGTCC ACCACAGCAA CTGACTGCAC AGTTAGAAAT CCATAACTCA 
GAAGAACCCA AGATAGAGGT TGATATTGTT ACTATGGCAG TGCCATTGGA TAGTAAATCA 
GCTCAGCATT ACGGCAATTA CTTGCTTGTA CCTTCAGTGG GGCCCCCTGC ACCAACTGAT 
TCTGCCTTTA AAGAGACATG TTCAGATATG CTCAGTGAAG GGGAAGCAAT ATCTGTACAA 
GCTACAACCA AAGAGAGGAT ATGTTCCATT CATTCTGACC AGTGTCTCTG TAGCAAACAA 
AAGCATGTGA AGGAAAGTAG TCGCAAGACC ATGAAAGAGG ATTTAAAGCC TCTTGTGACA 
AATACTGATG AAAGCCAAAA TGAAGAAAGC CATGAATGCT CTTGCGATCA GTTTACTGCT 
CATGTCACTG TTGAGACCTC AATTACGTGC AACATTATTG ACCCCGGAGG TGGTTATGTA 
AACTTGGCAT TTTCAGAAGA AGGAGAAACT GCCCTGTTTA GTGGAGGGAG TAGCAAGACC 
CTTTGCTCAC GTCCCACCCA AAGTTCAAAA TCTAATGTGT TTCCAAATAA GTCTGATGGA 
TCTAGAGCCA ATGATAGGCC TGAGTTTGGT TCAGGTTACA AACAGAAAAC TTCATTCCTG 
AAAAATTCAC TTTATAGGAA TAACTCACCA AGAGGCAGTT CTAAGAGGCA TAAAGAGAAG 
AAAGATGGAA AGATATGTAC AATCAAAGAT TTTGCCAAGC CTCCTGAGAG CTCTAAGGTT 
ACATGGGCCA AAGAGAAACT GCGTAGCACA TTCAGAAAGT CTGTTAAAGA CAAGCCAAGG 
CCAACTCAAC TTCAAGTTCC AGTGATAACT GTGGATGGTT GCTCTCAGAG TACGCAGATG 
AGTTCCGAAC CTGCAGATTG TGGCAGCTGG AAATCTGAAG ATGAACCCAG TAAGAACAGG 
CTTACAGTAT CAAGTTTCAA TCAGCTAAGC TTGGATCACA TGACTTACAT GCGACAGCAA 
CTAAAGACGC AAGAATTTGA AAGACACCTA GTTCAGACTA GTGAATATCT AAGGCAGTCC 
AGAGAGGATT TGCATAACAC CACCCAACTG AGAAGGAGCT CATTATTCCG AAATTCCGAT 
GAAGTAAAGA AAATGGCAGC TTCCCTAAAA GCCTCTCAGG ATTTAAGTAA TCATTATTCA 
GCGGTGGAAA GGAATAATTT AATGAGGCTT GCACAGAGCA TACCTTTCAC CCCCCTGCAC 
CTTTTTGCTG GAGAAGAAGT CACTGTTTAC CAACTAGAGG AAAGTTCTCC AGCAATTCTA 
GATAAAAGTA TGTCTTCCTG GTCACAGCGT GGAATGGCAG CAATGCTTCA GGTTCTGTCC 
CCAGAGGAAA TGGATGGAGG TCTCCGCAGG GCGTTAAAAG TTGTGTGCAC TTGGTCTGAG 
CATGATGTAC TGACGCCTGG TGAGGTGTTT ATAGTCAAAT CTTTTCTTCC GGAGGTAGTA 
CAGACTTGGC AGAAGGTTTT CCGTGGTGAT ACTGTACTGA ATCTGTGTCT CAGGGAAATT 
CAGCAACAAA GAGCAGCTCA AAAGTTGATC TACACCTTCA ATCAAGTGAA ACCACACAGT 
ATTCCTTATA CTCTAAGGTT TCTAGAAGTA TTTCTTATAT TCTGCCATTC AGCTAACCAA 
TGGCTTACTA TAGAGAAGTA CCTGACTGGA GAATTCCGCA AGTACAATAA CAACAATGGA 
GATGAAATTA CCCCCACCAC TTTACTTGAG GAGACACTGC TGTCTTTTTC TCATTGGAGT 
TATGAATATA CAAGGGGAGA GCTGCTTGTT TTGGATTTAC AAGGCGTTGG GGAAAATCTT 
ACAGACCCAT CTGTTATTAA ACCAGAAGAC AAACAATCAA GAGGAATGGT ATTTGGACCA 
GCTAATTTAG GAGAAGATGC TCTGACTAAT TATGTTTCAA AGCATCGCTG CAATTCCTGC 
TGCAGAAAGC TTAAACTCCT TGATTTGAAA AGAAATGACT ACTCACCTGG GAGGATCAAT 
CTAGGGTTTG AAATTGGAGA AGCAGACACA GATAAAGACC TTGTGGAATA TAATACAAGA 
CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpm6 ( XM_031890874.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746734.1 Xenopus tropicalis transient receptor potential cation channel, subfamily M, member 6 (trpm6), transcript variant X1, mRNA.

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CloneID	OXa48738
Clone ID Related Accession (Same CDS sequence)	XM_031890874.1
Accession Version	XM_031890874.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6111bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transient receptor potential cation channel subfamily M member 6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTCC AGCTAAAGTC TCAGAAATCA TGGATTGAAG GGCTCTTCAA TAAAAGAGAA 
TGTGCAAAAA TCATACCGTC ATCAAAAGAG CCACACAGAT GCCACGGTGG ATGTCAGGTT 
TGCCAGAATT TAATAAGGTG TTGTTGTGGA CGGTTAATTG GAGACCACCC TGGGTTAGAT 
TATGGATGGC CAATTAATCC TTCTCCCCAA GAAAGAGAAG ACGAAGAGTG GAGTATTCTA 
AATCATACAA AACCAAGTCC CACTGATGCA TTTGGCACAA TAAATTTTCA AGATGGAGAT 
CACACCTATC ATGCAAAGTA CATAAGAATA TCCTATGATA CCACATTGGA ACTGTTAATG 
CATCTGATGA TTAAAGAATG GCAAATGGAA CTGCCCAAGC TTGTGATCTC TGTCCATGGG 
GGCATACAGA ATTTTAAACT TCCGTCCAAA ATAAAGCAAG TTTTTGGCAA AGGATTGGTG 
AAAGCTGCTG AGACAACAGG AGCATGGATC CTTACAGAGG GCATTAATAC TGGGGCCTCC 
AAACATGTGG GTGATGCTTT AAAAGCACAC GCTTCTCAAC ACCTGCGAAA AATCTGTGCA 
GTTGGCATAC CACCATGGGG AGCTATTGAG AATCAGCAGG ATCTCATTGG CAAAGACGTT 
GTGTGCCTGT ATCAGACACT GATAAATCCA ATGAGCAAAC TGACAAGTCT CAATAGCATG 
CACTCCCATT TTATAATGGT GGATGATGGC ACAGTTGGAA AGTATGGTAA TGAAATGAAA 
TTAAGGAGAA ACTTGGAAGA CTTCATTTCC CTTCAGAAGA TCCATACCCG GATGGGGCAA 
GGAGTGCCTG TGGTAGGGCT TGTTATAGAA GGTGGTCCAA ATGTCATCTT GATGGTATGG 
GAATATGTGA GGAGCACTCC ACCAGTACCA GTTGTAGTGT GTGAAGGAAC AGGCAGAGCA 
GCTGATATAT TGGCATTTAC TCACAAGCAC ACAACGGACA CAGAGCAGAT TAGTCCACAG 
CTGAAAGAAG AAATCTTAGA GATGATTCAG AAGACATTTA ATCTTGGCCA CAGACAATCA 
AACCATGTTC TTTATATTCT AATGGAGTGT ATGGAGCGCC GTGCGTCTAT TACTATATTT 
GATGCAGAAT CTGAAGAACA GCAAGATATT GATTTGGCTA TATTGACAGC TCTGCTAAAA 
GGGACTAATA TGTCTGCTCA AGACCAGCTG AGCTTGGCGC TAACCTGGAA CAGGGTTGAC 
ATTGCCAAAA AACACATACT TATATATGGA CAACATTGGA AGGTGGGGTC TTTGGAACAA 
GCAATGCAAG ATGCACTTGT AATGGATCGG GTGGATTTTG TAAAGTTGCT AATTGAATAT 
GGTGTGAATA TGCATCGCTT CTTGACCATT GGTCATTTGG AAGAATTATA CAATACTAAA 
CAAGGCCCCA CAAATCTGCT CCTACACCAT TTAGTCAGAG ATGTAAAACA GAGCAATCCT 
TCTCCAGATT ACAGAATTTC CCTTATAGAT GTGGGACTTG TAATCGAGTA CTTGATTGGT 
GGAGCCTACC GAAGCAGTTA TACAAGGAAA AGTTTCAGAG TCCTGTACAA CAGTCTCTTC 
AGGAAGCATA AGAAATCTGT GCCCAGTTCC TCCAGCAGCC TTGCACAAAG TTTATCTCAT 
CTTTCCATAA ACCAGAGCAG CCAGATGAGC AGCAAGCTTG AATCGCCAGA GAGTACCTTG 
CATTCCCAAT TTTTCCGAAC AGCCCAACCT TGTAGACGCA AGGACAAGAC AACATGCCAT 
CAAAAGTCAA AAAAGAAAGA TGAAGTAACT TGCACAGAAG ATAATGAACC AGTTGGCTTT 
ATCTACCCTT ACAATGACCT CTTGGTTTGG GCTGTACTGA TGAAAAGACA GAAGATGGCT 
ATGTTTTTTT GGCAACATGG AGAGGAGGCC ATGGTCAAAG CAGTAATTGC CTGTAAGCTA 
TATAGGGCCA TGGCACATGA AGCTAAGCAG AGCACCATGG TGGATGACAC TTCAGAAGAA 
TTAAAGAAAT ATTCAAAAGA ATTTGGACAG CTTGCTTTGG ACATACTTGA AAATGCTTTC 
AAAATGAATG AACAAATGGC CATGAAACTT TTAACATACC AGTTAAAGAA CTGGAGTAAT 
TCAACGTGCC TGAAATTAGC GGTTTCATCA ACATTGCGGC CCTTTGTATC CCATACATGC 
ACACAGATGT TACTGACAGA CATGTGGATG GGTCGCCTGA AGATGCGGAA ACACTCCTGG 
CTTAAAGTCA TTCTAGGAAT ATTGCTGCCA CCCTTTGTCT TGATGTTGGA ATTTAAGACC 
AAGGCAGAAA TGTCCCATGT ACCCCAGTCA CTAGAGTTCC ATCAGTTCTC ACGGCTGTAC 
GTTGACCCTC CATCACTGAG CACCAAAGAG GAACATATTA CAGATTTTGA TGCTGAAAGG 
GGATCAAAGC AGCCAGGAGA AGATCAAAAT CAGAAGATCA GTAATGGTCT AGGAATCCTT 
CCGATAACCA GGAAAATTTG TGAATTTTAT AGCGCTCCCA TTGTCAAGTT CTGGTTCCAC 
ACTATGGTGT ACTTGGCATT CCTCATGCTT TTCACATACA CGGTGCTGGT AAAGATGGAC 
CCACAGCCCA GTGTCCAAGA GTGGTGTGTA ATAATTTATA TATTCACTAC GGCAGTTGAA 
AAAGTCAGGG AGGTATTTGT GTCAGAACCT GGAAAGTTCA GTCAAAAAGT AAAAGTTTGG 
ATCAATGAGT ACTGGAATCT TACAGATTCT ATTGCAATTT TCTTGTTTTT TATTGGTTTC 
GGCTTGCGCT GGCCCGACCC TCCGTTTCAA ACAGCAGGGC GCATAATATA TTGCCTGGAC 
ATAATATTTT GGTATGTGCG ACTTTTAGAC TTCTTTGCAG TGAACCAGCA AGCAGGCCCT 
TACATTACAA TGCTTGGAAA AATGACATCC AACATGTTTG GTATTGTGAT TATAATGGCA 
ATTGTTCTGT TGGCATTTGG GGTGTCCCGG AAAGCAATTC TGTCTCCCGA TCACCCTCCT 
TCTTGGACTC TTGCTCGTGA TATAGTGTTT CAGCCATACT GGATGATGTT TGGGGAAGTA 
TATGCTGGGG AAATTGATGC CTGTGAAGAA GATCCAGATT GCCCTCCTGG TTCCTTCATC 
ACCCCATTCC TTCAAGCTGT GTATCTCTTT GTGCAGTATA TCATCATGGT CAACCTGCTT 
ATTTCTCTGT TTAATAATGT CTACAGGGTA TTAAACTCTG TCTCTCACAA ACTTTGGAAA 
TACAATCGAT ATCGATATAT AATGACATAC TACGAGAAGC CATGGCTACC TCCACCCCTG 
ATATTCCTTA GTCATATATA TATACTTATC AATCGCCTTT GTCATCACCG ATCTCACAGT 
GAGTTTGACC CAGAGGAAAC TGACATGGGA CTGAAGCTGT ACCTTAGTGA TAATGAATTG 
AGGAAACTCC ATGATTTTGA GGAAAAATGT GTGGAAACAT ACTTCCATGA GAAAAATGAA 
AGCCTGCACT CCAGCAAAAA TGAAAGAATC CGTGTAACAA CAGAGAGAAC TGAAGAGATG 
TTTGCCCAAC TGAAAGAAGT TAATGATAAA GTTTTCTACA TCAAAGACTC CTTGAGCTCC 
CTGGACAGCC AATTAGGGCA TTTGCAGGAT CTCTCCGCAT TGACTGTGGA TACTTTAAAA 
ATCATTTCAG CTGTGGATTC TTTGCAAGTT GAAGAAGCCT TTATAAAAGA CAAGAAAGGA 
CTCACCTGTA AAAAGCTCCC ACACAGTTGG AGCAACGTTC CATATTCTCA TCACCAGAGA 
GAGTATAATT ACTACAGTAT GCCACCTTCA CTTCTCCGGA CCTTGGCCAG GAGTCAGTGC 
CCATCACTGT ATCAATGTCC ACCACAGCAA CTGACTGCAC AGTTAGAAAT CCATAACTCA 
GAAGAACCCA AGATAGAGGT TGATATTGTT ACTATGGCAG TGCCATTGGA TAGTAAATCA 
GCTCAGCATT ACGGCAATTA CTTGCTTGTA CCTTCAGTGG GGCCCCCTGC ACCAACTGAT 
TCTGCCTTTA AAGAGACATG TTCAGATATG CTCAGTGAAG GGGAAGCAAT ATCTGTACAA 
GCTACAACCA AAGAGAGGAT ATGTTCCATT CATTCTGACC AGTGTCTCTG TAGCAAACAA 
AAGCATGTGA AGGAAAGTAG TCGCAAGACC ATGAAAGAGG ATTTAAAGCC TCTTGTGACA 
AATACTGATG AAAGCCAAAA TGAAGAAAGC CATGAATGCT CTTGCGATCA GTTTACTGCT 
CATGTCACTG TTGAGACCTC AATTACGTGC AACATTATTG ACCCCGGAGG TGGTTATGTA 
AACTTGGCAT TTTCAGAAGA AGGAGAAACT GCCCTGTTTA GTGGAGGGAG TAGCAAGACC 
CTTTGCTCAC GTCCCACCCA AAGTTCAAAA TCTAATGTGT TTCCAAATAA GTCTGATGGA 
TCTAGAGCCA ATGATAGGCC TGAGTTTGGT TCAGGTTACA AACAGAAAAC TTCATTCCTG 
AAAAATTCAC TTTATAGGAA TAACTCACCA AGAGGCAGTT CTAAGAGGCA TAAAGAGAAG 
AAAGATGGAA AGATATGTAC AATCAAAGAT TTTGCCAAGC CTCCTGAGAG CTCTAAGGTT 
ACATGGGCCA AAGAGAAACT GCGTAGCACA TTCAGAAAGT CTGTTAAAGA CAAGCCAAGG 
CCAACTCAAC TTCAAGTTCC AGTGATAACT GTGGATGGTT GCTCTCAGAG TACGCAGATG 
AGTTCCGAAC CTGCAGATTG TGGCAGCTGG AAATCTGAAG ATGAACCCAG TAAGAACAGG 
CTTACAGTAT CAAGTTTCAA TCAGCTAAGC TTGGATCACA TGACTTACAT GCGACAGCAA 
CTAAAGACGC AAGAATTTGA AAGACACCTA GTTCAGACTA GTGAATATCT AAGGCAGTCC 
AGAGAGGATT TGCATAACAC CACCCAACTG AGAAGGAGCT CATTATTCCG AAATTCCGAT 
GAAGTAAAGA AAATGGCAGC TTCCCTAAAA GCCTCTCAGG ATTTAAGTAA TCATTATTCA 
GCGGTGGAAA GGAATAATTT AATGAGGCTT GCACAGAGCA TACCTTTCAC CCCCCTGCAC 
CTTTTTGCTG GAGAAGAAGT CACTGTTTAC CAACTAGAGG AAAGTTCTCC AGCAATTCTA 
GATAAAAGTA TGTCTTCCTG GTCACAGCGT GGAATGGCAG CAATGCTTCA GGTTCTGTCC 
CCAGAGGAAA TGGATGGAGG TCTCCGCAGG GCGTTAAAAG TTGTGTGCAC TTGGTCTGAG 
CATGATGTAC TGACGCCTGG TGAGGTGTTT ATAGTCAAAT CTTTTCTTCC GGAGGTAGTA 
CAGACTTGGC AGAAGGTTTT CCGTGGTGAT ACTGTACTGA ATCTGTGTCT CAGGGAAATT 
CAGCAACAAA GAGCAGCTCA AAAGTTGATC TACACCTTCA ATCAAGTGAA ACCACACAGT 
ATTCCTTATA CTCTAAGGTT TCTAGAAGTA TTTCTTATAT TCTGCCATTC AGCTAACCAA 
TGGCTTACTA TAGAGAAGTA CCTGACTGGA GAATTCCGCA AGTACAATAA CAACAATGGA 
GATGAAATTA CCCCCACCAC TTTACTTGAG GAGACACTGC TGTCTTTTTC TCATTGGAGT 
TATGAATATA CAAGGGGAGA GCTGCTTGTT TTGGATTTAC AAGGCGTTGG GGAAAATCTT 
ACAGACCCAT CTGTTATTAA ACCAGAAGAC AAACAATCAA GAGGAATGGT ATTTGGACCA 
GCTAATTTAG GAGAAGATGC TCTGACTAAT TATGTTTCAA AGCATCGCTG CAATTCCTGC 
TGCAGAAAGC TTAAACTCCT TGTGCACTCA TTCTGTCTCA CCTTGTTTTT GAGAACAGTA 
GCAGCTGTAG CAGCCGAAAC GTTAGATGTA TTCCTATCAA TAAATGTTTT GATTGACCAT 
AAGTCCTATT TGAAAAGAAA TGACTACTCA CCTGGGAGGA TCAATCTAGG GTTTGAAATT 
GGAGAAGCAG ACACAGATAA AGACCTTGTG GAATATAATA CAAGACTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746734.1
CDS	291..6401
Translation	MKVQLKSQKSWIEGLFNKRECAKIIPSSKEPHRCHGGCQVCQNLIRCCCGRLIGDHPGLDYGWPINPSPQEREDEEWSILNHTKPSPTDAFGTINFQDGDHTYHAKYIRISYDTTLELLMHLMIKEWQMELPKLVISVHGGIQNFKLPSKIKQVFGKGLVKAAETTGAWILTEGINTGASKHVGDALKAHASQHLRKICAVGIPPWGAIENQQDLIGKDVVCLYQTLINPMSKLTSLNSMHSHFIMVDDGTVGKYGNEMKLRRNLEDFISLQKIHTRMGQGVPVVGLVIEGGPNVILMVWEYVRSTPPVPVVVCEGTGRAADILAFTHKHTTDTEQISPQLKEEILEMIQKTFNLGHRQSNHVLYILMECMERRASITIFDAESEEQQDIDLAILTALLKGTNMSAQDQLSLALTWNRVDIAKKHILIYGQHWKVGSLEQAMQDALVMDRVDFVKLLIEYGVNMHRFLTIGHLEELYNTKQGPTNLLLHHLVRDVKQSNPSPDYRISLIDVGLVIEYLIGGAYRSSYTRKSFRVLYNSLFRKHKKSVPSSSSSLAQSLSHLSINQSSQMSSKLESPESTLHSQFFRTAQPCRRKDKTTCHQKSKKKDEVTCTEDNEPVGFIYPYNDLLVWAVLMKRQKMAMFFWQHGEEAMVKAVIACKLYRAMAHEAKQSTMVDDTSEELKKYSKEFGQLALDILENAFKMNEQMAMKLLTYQLKNWSNSTCLKLAVSSTLRPFVSHTCTQMLLTDMWMGRLKMRKHSWLKVILGILLPPFVLMLEFKTKAEMSHVPQSLEFHQFSRLYVDPPSLSTKEEHITDFDAERGSKQPGEDQNQKISNGLGILPITRKICEFYSAPIVKFWFHTMVYLAFLMLFTYTVLVKMDPQPSVQEWCVIIYIFTTAVEKVREVFVSEPGKFSQKVKVWINEYWNLTDSIAIFLFFIGFGLRWPDPPFQTAGRIIYCLDIIFWYVRLLDFFAVNQQAGPYITMLGKMTSNMFGIVIIMAIVLLAFGVSRKAILSPDHPPSWTLARDIVFQPYWMMFGEVYAGEIDACEEDPDCPPGSFITPFLQAVYLFVQYIIMVNLLISLFNNVYRVLNSVSHKLWKYNRYRYIMTYYEKPWLPPPLIFLSHIYILINRLCHHRSHSEFDPEETDMGLKLYLSDNELRKLHDFEEKCVETYFHEKNESLHSSKNERIRVTTERTEEMFAQLKEVNDKVFYIKDSLSSLDSQLGHLQDLSALTVDTLKIISAVDSLQVEEAFIKDKKGLTCKKLPHSWSNVPYSHHQREYNYYSMPPSLLRTLARSQCPSLYQCPPQQLTAQLEIHNSEEPKIEVDIVTMAVPLDSKSAQHYGNYLLVPSVGPPAPTDSAFKETCSDMLSEGEAISVQATTKERICSIHSDQCLCSKQKHVKESSRKTMKEDLKPLVTNTDESQNEESHECSCDQFTAHVTVETSITCNIIDPGGGYVNLAFSEEGETALFSGGSSKTLCSRPTQSSKSNVFPNKSDGSRANDRPEFGSGYKQKTSFLKNSLYRNNSPRGSSKRHKEKKDGKICTIKDFAKPPESSKVTWAKEKLRSTFRKSVKDKPRPTQLQVPVITVDGCSQSTQMSSEPADCGSWKSEDEPSKNRLTVSSFNQLSLDHMTYMRQQLKTQEFERHLVQTSEYLRQSREDLHNTTQLRRSSLFRNSDEVKKMAASLKASQDLSNHYSAVERNNLMRLAQSIPFTPLHLFAGEEVTVYQLEESSPAILDKSMSSWSQRGMAAMLQVLSPEEMDGGLRRALKVVCTWSEHDVLTPGEVFIVKSFLPEVVQTWQKVFRGDTVLNLCLREIQQQRAAQKLIYTFNQVKPHSIPYTLRFLEVFLIFCHSANQWLTIEKYLTGEFRKYNNNNGDEITPTTLLEETLLSFSHWSYEYTRGELLVLDLQGVGENLTDPSVIKPEDKQSRGMVFGPANLGEDALTNYVSKHRCNSCCRKLKLLVHSFCLTLFLRTVAAVAAETLDVFLSINVLIDHKSYLKRNDYSPGRINLGFEIGEADTDKDLVEYNTRL

Target ORF information:

RefSeq Version	XM_031890874.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis transient receptor potential cation channel, subfamily M, member 6 (trpm6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031890874.1

ORF Insert Sequence:

ATGAAAGTCC AGCTAAAGTC TCAGAAATCA TGGATTGAAG GGCTCTTCAA TAAAAGAGAA 
TGTGCAAAAA TCATACCGTC ATCAAAAGAG CCACACAGAT GCCACGGTGG ATGTCAGGTT 
TGCCAGAATT TAATAAGGTG TTGTTGTGGA CGGTTAATTG GAGACCACCC TGGGTTAGAT 
TATGGATGGC CAATTAATCC TTCTCCCCAA GAAAGAGAAG ACGAAGAGTG GAGTATTCTA 
AATCATACAA AACCAAGTCC CACTGATGCA TTTGGCACAA TAAATTTTCA AGATGGAGAT 
CACACCTATC ATGCAAAGTA CATAAGAATA TCCTATGATA CCACATTGGA ACTGTTAATG 
CATCTGATGA TTAAAGAATG GCAAATGGAA CTGCCCAAGC TTGTGATCTC TGTCCATGGG 
GGCATACAGA ATTTTAAACT TCCGTCCAAA ATAAAGCAAG TTTTTGGCAA AGGATTGGTG 
AAAGCTGCTG AGACAACAGG AGCATGGATC CTTACAGAGG GCATTAATAC TGGGGCCTCC 
AAACATGTGG GTGATGCTTT AAAAGCACAC GCTTCTCAAC ACCTGCGAAA AATCTGTGCA 
GTTGGCATAC CACCATGGGG AGCTATTGAG AATCAGCAGG ATCTCATTGG CAAAGACGTT 
GTGTGCCTGT ATCAGACACT GATAAATCCA ATGAGCAAAC TGACAAGTCT CAATAGCATG 
CACTCCCATT TTATAATGGT GGATGATGGC ACAGTTGGAA AGTATGGTAA TGAAATGAAA 
TTAAGGAGAA ACTTGGAAGA CTTCATTTCC CTTCAGAAGA TCCATACCCG GATGGGGCAA 
GGAGTGCCTG TGGTAGGGCT TGTTATAGAA GGTGGTCCAA ATGTCATCTT GATGGTATGG 
GAATATGTGA GGAGCACTCC ACCAGTACCA GTTGTAGTGT GTGAAGGAAC AGGCAGAGCA 
GCTGATATAT TGGCATTTAC TCACAAGCAC ACAACGGACA CAGAGCAGAT TAGTCCACAG 
CTGAAAGAAG AAATCTTAGA GATGATTCAG AAGACATTTA ATCTTGGCCA CAGACAATCA 
AACCATGTTC TTTATATTCT AATGGAGTGT ATGGAGCGCC GTGCGTCTAT TACTATATTT 
GATGCAGAAT CTGAAGAACA GCAAGATATT GATTTGGCTA TATTGACAGC TCTGCTAAAA 
GGGACTAATA TGTCTGCTCA AGACCAGCTG AGCTTGGCGC TAACCTGGAA CAGGGTTGAC 
ATTGCCAAAA AACACATACT TATATATGGA CAACATTGGA AGGTGGGGTC TTTGGAACAA 
GCAATGCAAG ATGCACTTGT AATGGATCGG GTGGATTTTG TAAAGTTGCT AATTGAATAT 
GGTGTGAATA TGCATCGCTT CTTGACCATT GGTCATTTGG AAGAATTATA CAATACTAAA 
CAAGGCCCCA CAAATCTGCT CCTACACCAT TTAGTCAGAG ATGTAAAACA GAGCAATCCT 
TCTCCAGATT ACAGAATTTC CCTTATAGAT GTGGGACTTG TAATCGAGTA CTTGATTGGT 
GGAGCCTACC GAAGCAGTTA TACAAGGAAA AGTTTCAGAG TCCTGTACAA CAGTCTCTTC 
AGGAAGCATA AGAAATCTGT GCCCAGTTCC TCCAGCAGCC TTGCACAAAG TTTATCTCAT 
CTTTCCATAA ACCAGAGCAG CCAGATGAGC AGCAAGCTTG AATCGCCAGA GAGTACCTTG 
CATTCCCAAT TTTTCCGAAC AGCCCAACCT TGTAGACGCA AGGACAAGAC AACATGCCAT 
CAAAAGTCAA AAAAGAAAGA TGAAGTAACT TGCACAGAAG ATAATGAACC AGTTGGCTTT 
ATCTACCCTT ACAATGACCT CTTGGTTTGG GCTGTACTGA TGAAAAGACA GAAGATGGCT 
ATGTTTTTTT GGCAACATGG AGAGGAGGCC ATGGTCAAAG CAGTAATTGC CTGTAAGCTA 
TATAGGGCCA TGGCACATGA AGCTAAGCAG AGCACCATGG TGGATGACAC TTCAGAAGAA 
TTAAAGAAAT ATTCAAAAGA ATTTGGACAG CTTGCTTTGG ACATACTTGA AAATGCTTTC 
AAAATGAATG AACAAATGGC CATGAAACTT TTAACATACC AGTTAAAGAA CTGGAGTAAT 
TCAACGTGCC TGAAATTAGC GGTTTCATCA ACATTGCGGC CCTTTGTATC CCATACATGC 
ACACAGATGT TACTGACAGA CATGTGGATG GGTCGCCTGA AGATGCGGAA ACACTCCTGG 
CTTAAAGTCA TTCTAGGAAT ATTGCTGCCA CCCTTTGTCT TGATGTTGGA ATTTAAGACC 
AAGGCAGAAA TGTCCCATGT ACCCCAGTCA CTAGAGTTCC ATCAGTTCTC ACGGCTGTAC 
GTTGACCCTC CATCACTGAG CACCAAAGAG GAACATATTA CAGATTTTGA TGCTGAAAGG 
GGATCAAAGC AGCCAGGAGA AGATCAAAAT CAGAAGATCA GTAATGGTCT AGGAATCCTT 
CCGATAACCA GGAAAATTTG TGAATTTTAT AGCGCTCCCA TTGTCAAGTT CTGGTTCCAC 
ACTATGGTGT ACTTGGCATT CCTCATGCTT TTCACATACA CGGTGCTGGT AAAGATGGAC 
CCACAGCCCA GTGTCCAAGA GTGGTGTGTA ATAATTTATA TATTCACTAC GGCAGTTGAA 
AAAGTCAGGG AGGTATTTGT GTCAGAACCT GGAAAGTTCA GTCAAAAAGT AAAAGTTTGG 
ATCAATGAGT ACTGGAATCT TACAGATTCT ATTGCAATTT TCTTGTTTTT TATTGGTTTC 
GGCTTGCGCT GGCCCGACCC TCCGTTTCAA ACAGCAGGGC GCATAATATA TTGCCTGGAC 
ATAATATTTT GGTATGTGCG ACTTTTAGAC TTCTTTGCAG TGAACCAGCA AGCAGGCCCT 
TACATTACAA TGCTTGGAAA AATGACATCC AACATGTTTG GTATTGTGAT TATAATGGCA 
ATTGTTCTGT TGGCATTTGG GGTGTCCCGG AAAGCAATTC TGTCTCCCGA TCACCCTCCT 
TCTTGGACTC TTGCTCGTGA TATAGTGTTT CAGCCATACT GGATGATGTT TGGGGAAGTA 
TATGCTGGGG AAATTGATGC CTGTGAAGAA GATCCAGATT GCCCTCCTGG TTCCTTCATC 
ACCCCATTCC TTCAAGCTGT GTATCTCTTT GTGCAGTATA TCATCATGGT CAACCTGCTT 
ATTTCTCTGT TTAATAATGT CTACAGGGTA TTAAACTCTG TCTCTCACAA ACTTTGGAAA 
TACAATCGAT ATCGATATAT AATGACATAC TACGAGAAGC CATGGCTACC TCCACCCCTG 
ATATTCCTTA GTCATATATA TATACTTATC AATCGCCTTT GTCATCACCG ATCTCACAGT 
GAGTTTGACC CAGAGGAAAC TGACATGGGA CTGAAGCTGT ACCTTAGTGA TAATGAATTG 
AGGAAACTCC ATGATTTTGA GGAAAAATGT GTGGAAACAT ACTTCCATGA GAAAAATGAA 
AGCCTGCACT CCAGCAAAAA TGAAAGAATC CGTGTAACAA CAGAGAGAAC TGAAGAGATG 
TTTGCCCAAC TGAAAGAAGT TAATGATAAA GTTTTCTACA TCAAAGACTC CTTGAGCTCC 
CTGGACAGCC AATTAGGGCA TTTGCAGGAT CTCTCCGCAT TGACTGTGGA TACTTTAAAA 
ATCATTTCAG CTGTGGATTC TTTGCAAGTT GAAGAAGCCT TTATAAAAGA CAAGAAAGGA 
CTCACCTGTA AAAAGCTCCC ACACAGTTGG AGCAACGTTC CATATTCTCA TCACCAGAGA 
GAGTATAATT ACTACAGTAT GCCACCTTCA CTTCTCCGGA CCTTGGCCAG GAGTCAGTGC 
CCATCACTGT ATCAATGTCC ACCACAGCAA CTGACTGCAC AGTTAGAAAT CCATAACTCA 
GAAGAACCCA AGATAGAGGT TGATATTGTT ACTATGGCAG TGCCATTGGA TAGTAAATCA 
GCTCAGCATT ACGGCAATTA CTTGCTTGTA CCTTCAGTGG GGCCCCCTGC ACCAACTGAT 
TCTGCCTTTA AAGAGACATG TTCAGATATG CTCAGTGAAG GGGAAGCAAT ATCTGTACAA 
GCTACAACCA AAGAGAGGAT ATGTTCCATT CATTCTGACC AGTGTCTCTG TAGCAAACAA 
AAGCATGTGA AGGAAAGTAG TCGCAAGACC ATGAAAGAGG ATTTAAAGCC TCTTGTGACA 
AATACTGATG AAAGCCAAAA TGAAGAAAGC CATGAATGCT CTTGCGATCA GTTTACTGCT 
CATGTCACTG TTGAGACCTC AATTACGTGC AACATTATTG ACCCCGGAGG TGGTTATGTA 
AACTTGGCAT TTTCAGAAGA AGGAGAAACT GCCCTGTTTA GTGGAGGGAG TAGCAAGACC 
CTTTGCTCAC GTCCCACCCA AAGTTCAAAA TCTAATGTGT TTCCAAATAA GTCTGATGGA 
TCTAGAGCCA ATGATAGGCC TGAGTTTGGT TCAGGTTACA AACAGAAAAC TTCATTCCTG 
AAAAATTCAC TTTATAGGAA TAACTCACCA AGAGGCAGTT CTAAGAGGCA TAAAGAGAAG 
AAAGATGGAA AGATATGTAC AATCAAAGAT TTTGCCAAGC CTCCTGAGAG CTCTAAGGTT 
ACATGGGCCA AAGAGAAACT GCGTAGCACA TTCAGAAAGT CTGTTAAAGA CAAGCCAAGG 
CCAACTCAAC TTCAAGTTCC AGTGATAACT GTGGATGGTT GCTCTCAGAG TACGCAGATG 
AGTTCCGAAC CTGCAGATTG TGGCAGCTGG AAATCTGAAG ATGAACCCAG TAAGAACAGG 
CTTACAGTAT CAAGTTTCAA TCAGCTAAGC TTGGATCACA TGACTTACAT GCGACAGCAA 
CTAAAGACGC AAGAATTTGA AAGACACCTA GTTCAGACTA GTGAATATCT AAGGCAGTCC 
AGAGAGGATT TGCATAACAC CACCCAACTG AGAAGGAGCT CATTATTCCG AAATTCCGAT 
GAAGTAAAGA AAATGGCAGC TTCCCTAAAA GCCTCTCAGG ATTTAAGTAA TCATTATTCA 
GCGGTGGAAA GGAATAATTT AATGAGGCTT GCACAGAGCA TACCTTTCAC CCCCCTGCAC 
CTTTTTGCTG GAGAAGAAGT CACTGTTTAC CAACTAGAGG AAAGTTCTCC AGCAATTCTA 
GATAAAAGTA TGTCTTCCTG GTCACAGCGT GGAATGGCAG CAATGCTTCA GGTTCTGTCC 
CCAGAGGAAA TGGATGGAGG TCTCCGCAGG GCGTTAAAAG TTGTGTGCAC TTGGTCTGAG 
CATGATGTAC TGACGCCTGG TGAGGTGTTT ATAGTCAAAT CTTTTCTTCC GGAGGTAGTA 
CAGACTTGGC AGAAGGTTTT CCGTGGTGAT ACTGTACTGA ATCTGTGTCT CAGGGAAATT 
CAGCAACAAA GAGCAGCTCA AAAGTTGATC TACACCTTCA ATCAAGTGAA ACCACACAGT 
ATTCCTTATA CTCTAAGGTT TCTAGAAGTA TTTCTTATAT TCTGCCATTC AGCTAACCAA 
TGGCTTACTA TAGAGAAGTA CCTGACTGGA GAATTCCGCA AGTACAATAA CAACAATGGA 
GATGAAATTA CCCCCACCAC TTTACTTGAG GAGACACTGC TGTCTTTTTC TCATTGGAGT 
TATGAATATA CAAGGGGAGA GCTGCTTGTT TTGGATTTAC AAGGCGTTGG GGAAAATCTT 
ACAGACCCAT CTGTTATTAA ACCAGAAGAC AAACAATCAA GAGGAATGGT ATTTGGACCA 
GCTAATTTAG GAGAAGATGC TCTGACTAAT TATGTTTCAA AGCATCGCTG CAATTCCTGC 
TGCAGAAAGC TTAAACTCCT TGTGCACTCA TTCTGTCTCA CCTTGTTTTT GAGAACAGTA 
GCAGCTGTAG CAGCCGAAAC GTTAGATGTA TTCCTATCAA TAAATGTTTT GATTGACCAT 
AAGTCCTATT TGAAAAGAAA TGACTACTCA CCTGGGAGGA TCAATCTAGG GTTTGAAATT 
GGAGAAGCAG ACACAGATAA AGACCTTGTG GAATATAATA CAAGACTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpm6 ( XM_002940033.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002940079.2 Xenopus tropicalis transient receptor potential cation channel, subfamily M, member 6 (trpm6), transcript variant X1, mRNA.

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CloneID	OXa05105
Clone ID Related Accession (Same CDS sequence)	XM_002940033.4 , XM_002940033.5
Accession Version	XM_002940033.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6006bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transient receptor potential cation channel subfamily M member 6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002940033.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTCC AGCTAAAGTC TCAGAAATCA TGGATTGAAG GGCTCTTCAA TAAAAGAGAA 
TGTGCAAAAA TCATACCGTC ATCAAAAGAG CCACACAGAT GCCACGGTGG ATGTCAGGTT 
TGCCAGAATT TAATAAGGTG TTGTTGTGGA CGGTTAATTG GAGACCACCC TGGGTTAGAT 
TATGGATGGC CAATTAATCC TTCTCCCCAA GAAAGAGAAG ACGAAGAGTG GAGTATTCTA 
AATCATACAA AACCAAGTCC CACTGATGCA TTTGGCACAA TAAATTTTCA AGATGGAGAT 
CACACCTATC ATGCAAAGTA CATAAGAATA TCCTATGATA CCACATTGGA ACTGTTAATG 
CATCTGATGA TTAAAGAATG GCAAATGGAA CTGCCCAAGC TTGTGATCTC TGTCCATGGG 
GGCATACAGA ATTTTAAACT TCCGTCCAAA ATAAAGCAAG TTTTTGGCAA AGGATTGGTG 
AAAGCTGCTG AGACAACAGG AGCATGGATC CTTACAGAGG GCATTAATAC TGGGGCCTCC 
AAACATGTGG GTGATGCTTT AAAAGCACAC GCTTCTCAAC ACCTGCGAAA AATCTGTGCA 
GTTGGCATAC CACCATGGGG AGCTATTGAG AATCAGCAGG ATCTCATTGG CAAAGACGTT 
GTGTGCCTGT ATCAGACACT GATAAATCCA ATGAGCAAAC TGACAAGTCT CAATAGCATG 
CACTCCCATT TTATAATGGT GGATGATGGC ACAGTTGGAA AGTATGGTAA TGAAATGAAA 
TTAAGGAGAA ACTTGGAAGA CTTCATTTCC CTTCAGAAGA TCCATACCCG GATGGGGCAA 
GGAGTGCCTG TGGTAGGGCT TGTTATAGAA GGTGGTCCAA ATGTCATCTT GATGGTATGG 
GAATATGTGA GGAGCACTCC ACCAGTACCA GTTGTAGTGT GTGAAGGAAC AGGCAGAGCA 
GCTGATATAT TGGCATTTAC TCACAAGCAC ACAACGGACA CAGAGCAGAT TAGTCCACAG 
CTGAAAGAAG AAATCTTAGA GATGATTCAG AAGACATTTA ATCTTGGCCA CAGACAATCA 
AACCATGTTC TTTATATTCT AATGGAGTGT ATGGAGCGCC GTGCGTCTAT TACTATATTT 
GATGCAGAAT CTGAAGAACA GCAAGATATT GATTTGGCTA TATTGACAGC TCTGCTAAAA 
GGGACTAATA TGTCTGCTCA AGACCAGCTG AGCTTGGCGC TAACCTGGAA CAGGGTTGAC 
ATTGCCAAAA AACACATACT TATATATGGA CAACATTGGA AGGTGGGGTC TTTGGAACAA 
GCAATGCAAG ATGCACTTGT AATGGATCGG GTGGATTTTG TAAAGTTGCT AATTGAATAT 
GGTGTGAATA TGCATCGCTT CTTGACCATT GGTCATTTGG AAGAATTATA CAATACTAAA 
CAAGGCCCCA CAAATCTGCT CCTACACCAT TTAGTCAGAG ATGTAAAACA GAGCAATCCT 
TCTCCAGATT ACAGAATTTC CCTTATAGAT GTGGGACTTG TAATCGAGTA CTTGATTGGT 
GGAGCCTACC GAAGCAGTTA TACAAGGAAA AGTTTCAGAG TCCTGTACAA CAGTCTCTTC 
AGGAAGCATA AGAAATCTGT GCCCAGTTCC TCCAGCAGCC TTGCACAAAG TTTATCTCAT 
CTTTCCATAA ACCAGAGCAG CCAGATGAGC AGCAAGCTTG AATCGCCAGA GAGTACCTTG 
CATTCCCAAT TTTTCCGAAC AGCCCAACCT TGTAGACGCA AGGACAAGAC AACATGCCAT 
CAAAAGTCAA AAAAGAAAGA TGAAGTAACT TGCACAGAAG ATAATGAACC AGTTGGCTTT 
ATCTACCCTT ACAATGACCT CTTGGTTTGG GCTGTACTGA TGAAAAGACA GAAGATGGCT 
ATGTTTTTTT GGCAACATGG AGAGGAGGCC ATGGTCAAAG CAGTAATTGC CTGTAAGCTA 
TATAGGGCCA TGGCACATGA AGCTAAGCAG AGCACCATGG TGGATGACAC TTCAGAAGAA 
TTAAAGAAAT ATTCAAAAGA ATTTGGACAG CTTGCTTTGG ACATACTTGA AAATGCTTTC 
AAAATGAATG AACAAATGGC CATGAAACTT TTAACATACC AGTTAAAGAA CTGGAGTAAT 
TCAACGTGCC TGAAATTAGC GGTTTCATCA ACATTGCGGC CCTTTGTATC CCATACATGC 
ACACAGATGT TACTGACAGA CATGTGGATG GGTCGCCTGA AGATGCGGAA ACACTCCTGG 
CTTAAAGTCA TTCTAGGAAT ATTGCTGCCA CCCTTTGTCT TGATGTTGGA ATTTAAGACC 
AAGGCAGAAA TGTCCCATGT ACCCCAGTCA CTAGAGTTCC ATCAGTTCTC ACGGCTGTAC 
GTTGACCCTC CATCACTGAG CACCAAAGAG GAACATATTA CAGATTTTGA TGCTGAAAGG 
GGATCAAAGC AGCCAGGAGA AGATCAAAAT CAGAAGATCA GTAATGGTCT AGGAATCCTT 
CCGATAACCA GGAAAATTTG TGAATTTTAT AGCGCTCCCA TTGTCAAGTT CTGGTTCCAC 
ACTATGGTGT ACTTGGCATT CCTCATGCTT TTCACATACA CGGTGCTGGT AAAGATGGAC 
CCACAGCCCA GTGTCCAAGA GTGGTGTGTA ATAATTTATA TATTCACTAC GGCAGTTGAA 
AAAGTCAGGG AGGTATTTGT GTCAGAACCT GGAAAGTTCA GTCAAAAAGT AAAAGTTTGG 
ATCAATGAGT ACTGGAATCT TACAGATTCT ATTGCAATTT TCTTGTTTTT TATTGGTTTC 
GGCTTGCGCT GGCCCGACCC TCCGTTTCAA ACAGCAGGGC GCATAATATA TTGCCTGGAC 
ATAATATTTT GGTATGTGCG ACTTTTAGAC TTCTTTGCAG TGAACCAGCA AGCAGGCCCT 
TACATTACAA TGCTTGGAAA AATGACATCC AACATGTTTG GTATTGTGAT TATAATGGCA 
ATTGTTCTGT TGGCATTTGG GGTGTCCCGG AAAGCAATTC TGTCTCCCGA TCACCCTCCT 
TCTTGGACTC TTGCTCGTGA TATAGTGTTT CAGCCATACT GGATGATGTT TGGGGAAGTA 
TATGCTGGGG AAATTGATGC CTGTGAAGAA GATCCAGATT GCCCTCCTGG TTCCTTCATC 
ACCCCATTCC TTCAAGCTGT GTATCTCTTT GTGCAGTATA TCATCATGGT CAACCTGCTT 
ATTTCTCTGT TTAATAATGT CTACAGGGTA TTAAACTCTG TCTCTCACAA ACTTTGGAAA 
TACAATCGAT ATCGATATAT AATGACATAC TACGAGAAGC CATGGCTACC TCCACCCCTG 
ATATTCCTTA GTCATATATA TATACTTATC AATCGCCTTT GTCATCACCG ATCTCACAGT 
GAGTTTGACC CAGAGGAAAC TGACATGGGA CTGAAGCTGT ACCTTAGTGA TAATGAATTG 
AGGAAACTCC ATGATTTTGA GGAAAAATGT GTGGAAACAT ACTTCCATGA GAAAAATGAA 
AGCCTGCACT CCAGCAAAAA TGAAAGAATC CGTGTAACAA CAGAGAGAAC TGAAGAGATG 
TTTGCCCAAC TGAAAGAAGT TAATGATAAA GTTTTCTACA TCAAAGACTC CTTGAGCTCC 
CTGGACAGCC AATTAGGGCA TTTGCAGGAT CTCTCCGCAT TGACTGTGGA TACTTTAAAA 
ATCATTTCAG CTGTGGATTC TTTGCAAGTT GAAGAAGCCT TTATAAAAGA CAAGAAAGGA 
CTCACCTGTA AAAAGCTCCC ACACAGTTGG AGCAACGTTC CATATTCTCA TCACCAGAGA 
GAGTATAATT ACTACAGTAT GCCACCTTCA CTTCTCCGGA CCTTGGCCAG GAGTCAGTGC 
CCATCACTGT ATCAATGTCC ACCACAGCAA CTGACTGCAC AGTTAGAAAT CCATAACTCA 
GAAGAACCCA AGATAGAGGT TGATATTGTT ACTATGGCAG TGCCATTGGA TAGTAAATCA 
GCTCAGCATT ACGGCAATTA CTTGCTTGTA CCTTCAGTGG GGCCCCCTGC ACCAACTGAT 
TCTGCCTTTA AAGAGACATG TTCAGATATG CTCAGTGAAG GGGAAGCAAT ATCTGTACAA 
GCTACAACCA AAGAGAGGAT ATGTTCCATT CATTCTGACC AGTGTCTCTG TAGCAAACAA 
AAGCATGTGA AGGAAAGTAG TCGCAAGACC ATGAAAGAGG ATTTAAAGCC TCTTGTGACA 
AATACTGATG AAAGCCAAAA TGAAGAAAGC CATGAATGCT CTTGCGATCA GTTTACTGCT 
CATGTCACTG TTGAGACCTC AATTACGTGC AACATTATTG ACCCCGGAGG TGGTTATGTA 
AACTTGGCAT TTTCAGAAGA AGGAGAAACT GCCCTGTTTA GTGGAGGGAG TAGCAAGACC 
CTTTGCTCAC GTCCCACCCA AAGTTCAAAA TCTAATGTGT TTCCAAATAA GTCTGATGGA 
TCTAGAGCCA ATGATAGGCC TGAGTTTGGT TCAGGTTACA AACAGAAAAC TTCATTCCTG 
AAAAATTCAC TTTATAGGAA TAACTCACCA AGAGGCAGTT CTAAGAGGCA TAAAGAGAAG 
AAAGATGGAA AGATATGTAC AATCAAAGAT TTTGCCAAGC CTCCTGAGAG CTCTAAGGTT 
ACATGGGCCA AAGAGAAACT GCGTAGCACA TTCAGAAAGT CTGTTAAAGA CAAGCCAAGG 
CCAACTCAAC TTCAAGTTCC AGTGATAACT GTGGATGGTT GCTCTCAGAG TACGCAGATG 
AGTTCCGAAC CTGCAGATTG TGGCAGCTGG AAATCTGAAG ATGAACCCAG TAAGAACAGG 
CTTACAGTAT CAAGTTTCAA TCAGCTAAGC TTGGATCACA TGACTTACAT GCGACAGCAA 
CTAAAGACGC AAGAATTTGA AAGACACCTA GTTCAGACTA GTGAATATCT AAGGCAGTCC 
AGAGAGGATT TGCATAACAC CACCCAACTG AGAAGGAGCT CATTATTCCG AAATTCCGAT 
GAAGTAAAGA AAATGGCAGC TTCCCTAAAA GCCTCTCAGG ATTTAAGTAA TCATTATTCA 
GCGGTGGAAA GGAATAATTT AATGAGGCTT GCACAGAGCA TACCTTTCAC CCCCCTGCAC 
CTTTTTGCTG GAGAAGAAGT CACTGTTTAC CAACTAGAGG AAAGTTCTCC AGCAATTCTA 
GATAAAAGTA TGTCTTCCTG GTCACAGCGT GGAATGGCAG CAATGCTTCA GGTTCTGTCC 
CCAGAGGAAA TGGATGGAGG TCTCCGCAGG GCGTTAAAAG TTGTGTGCAC TTGGTCTGAG 
CATGATGTAC TGACGCCTGG TGAGGTGTTT ATAGTCAAAT CTTTTCTTCC GGAGGTAGTA 
CAGACTTGGC AGAAGGTTTT CCGTGGTGAT ACTGTACTGA ATCTGTGTCT CAGGGAAATT 
CAGCAACAAA GAGCAGCTCA AAAGTTGATC TACACCTTCA ATCAAGTGAA ACCACACAGT 
ATTCCTTATA CTCTAAGGTT TCTAGAAGTA TTTCTTATAT TCTGCCATTC AGCTAACCAA 
TGGCTTACTA TAGAGAAGTA CCTGACTGGA GAATTCCGCA AGTACAATAA CAACAATGGA 
GATGAAATTA CCCCCACCAC TTTACTTGAG GAGACACTGC TGTCTTTTTC TCATTGGAGT 
TATGAATATA CAAGGGGAGA GCTGCTTGTT TTGGATTTAC AAGGCGTTGG GGAAAATCTT 
ACAGACCCAT CTGTTATTAA ACCAGAAGAC AAACAATCAA GAGGAATGGT ATTTGGACCA 
GCTAATTTAG GAGAAGATGC TCTGACTAAT TATGTTTCAA AGCATCGCTG CAATTCCTGC 
TGCAGAAAGC TTAAACTCCT TGATTTGAAA AGAAATGACT ACTCACCTGG GAGGATCAAT 
CTAGGGTTTG AAATTGGAGA AGCAGACACA GATAAAGACC TTGTGGAATA TAATACAAGA 
CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002940079.2
CDS	309..6314
Translation	MKVQLKSQKSWIEGLFNKRECAKIIPSSKEPHRCHGGCQVCQNLIRCCCGRLIGDHPGLDYGWPINPSPQEREDEEWSILNHTKPSPTDAFGTINFQDGDHTYHAKYIRISYDTTLELLMHLMIKEWQMELPKLVISVHGGIQNFKLPSKIKQVFGKGLVKAAETTGAWILTEGINTGASKHVGDALKAHASQHLRKICAVGIPPWGAIENQQDLIGKDVVCLYQTLINPMSKLTSLNSMHSHFIMVDDGTVGKYGNEMKLRRNLEDFISLQKIHTRMGQGVPVVGLVIEGGPNVILMVWEYVRSTPPVPVVVCEGTGRAADILAFTHKHTTDTEQISPQLKEEILEMIQKTFNLGHRQSNHVLYILMECMERRASITIFDAESEEQQDIDLAILTALLKGTNMSAQDQLSLALTWNRVDIAKKHILIYGQHWKVGSLEQAMQDALVMDRVDFVKLLIEYGVNMHRFLTIGHLEELYNTKQGPTNLLLHHLVRDVKQSNPSPDYRISLIDVGLVIEYLIGGAYRSSYTRKSFRVLYNSLFRKHKKSVPSSSSSLAQSLSHLSINQSSQMSSKLESPESTLHSQFFRTAQPCRRKDKTTCHQKSKKKDEVTCTEDNEPVGFIYPYNDLLVWAVLMKRQKMAMFFWQHGEEAMVKAVIACKLYRAMAHEAKQSTMVDDTSEELKKYSKEFGQLALDILENAFKMNEQMAMKLLTYQLKNWSNSTCLKLAVSSTLRPFVSHTCTQMLLTDMWMGRLKMRKHSWLKVILGILLPPFVLMLEFKTKAEMSHVPQSLEFHQFSRLYVDPPSLSTKEEHITDFDAERGSKQPGEDQNQKISNGLGILPITRKICEFYSAPIVKFWFHTMVYLAFLMLFTYTVLVKMDPQPSVQEWCVIIYIFTTAVEKVREVFVSEPGKFSQKVKVWINEYWNLTDSIAIFLFFIGFGLRWPDPPFQTAGRIIYCLDIIFWYVRLLDFFAVNQQAGPYITMLGKMTSNMFGIVIIMAIVLLAFGVSRKAILSPDHPPSWTLARDIVFQPYWMMFGEVYAGEIDACEEDPDCPPGSFITPFLQAVYLFVQYIIMVNLLISLFNNVYRVLNSVSHKLWKYNRYRYIMTYYEKPWLPPPLIFLSHIYILINRLCHHRSHSEFDPEETDMGLKLYLSDNELRKLHDFEEKCVETYFHEKNESLHSSKNERIRVTTERTEEMFAQLKEVNDKVFYIKDSLSSLDSQLGHLQDLSALTVDTLKIISAVDSLQVEEAFIKDKKGLTCKKLPHSWSNVPYSHHQREYNYYSMPPSLLRTLARSQCPSLYQCPPQQLTAQLEIHNSEEPKIEVDIVTMAVPLDSKSAQHYGNYLLVPSVGPPAPTDSAFKETCSDMLSEGEAISVQATTKERICSIHSDQCLCSKQKHVKESSRKTMKEDLKPLVTNTDESQNEESHECSCDQFTAHVTVETSITCNIIDPGGGYVNLAFSEEGETALFSGGSSKTLCSRPTQSSKSNVFPNKSDGSRANDRPEFGSGYKQKTSFLKNSLYRNNSPRGSSKRHKEKKDGKICTIKDFAKPPESSKVTWAKEKLRSTFRKSVKDKPRPTQLQVPVITVDGCSQSTQMSSEPADCGSWKSEDEPSKNRLTVSSFNQLSLDHMTYMRQQLKTQEFERHLVQTSEYLRQSREDLHNTTQLRRSSLFRNSDEVKKMAASLKASQDLSNHYSAVERNNLMRLAQSIPFTPLHLFAGEEVTVYQLEESSPAILDKSMSSWSQRGMAAMLQVLSPEEMDGGLRRALKVVCTWSEHDVLTPGEVFIVKSFLPEVVQTWQKVFRGDTVLNLCLREIQQQRAAQKLIYTFNQVKPHSIPYTLRFLEVFLIFCHSANQWLTIEKYLTGEFRKYNNNNGDEITPTTLLEETLLSFSHWSYEYTRGELLVLDLQGVGENLTDPSVIKPEDKQSRGMVFGPANLGEDALTNYVSKHRCNSCCRKLKLLDLKRNDYSPGRINLGFEIGEADTDKDLVEYNTRL

Target ORF information:

RefSeq Version	XM_002940033.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis transient receptor potential cation channel, subfamily M, member 6 (trpm6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002940033.4

ORF Insert Sequence:

ATGAAAGTCC AGCTAAAGTC TCAGAAATCA TGGATTGAAG GGCTCTTCAA TAAAAGAGAA 
TGTGCAAAAA TCATACCGTC ATCAAAAGAG CCACACAGAT GCCACGGTGG ATGTCAGGTT 
TGCCAGAATT TAATAAGGTG TTGTTGTGGA CGGTTAATTG GAGACCACCC TGGGTTAGAT 
TATGGATGGC CAATTAATCC TTCTCCCCAA GAAAGAGAAG ACGAAGAGTG GAGTATTCTA 
AATCATACAA AACCAAGTCC CACTGATGCA TTTGGCACAA TAAATTTTCA AGATGGAGAT 
CACACCTATC ATGCAAAGTA CATAAGAATA TCCTATGATA CCACATTGGA ACTGTTAATG 
CATCTGATGA TTAAAGAATG GCAAATGGAA CTGCCCAAGC TTGTGATCTC TGTCCATGGG 
GGCATACAGA ATTTTAAACT TCCGTCCAAA ATAAAGCAAG TTTTTGGCAA AGGATTGGTG 
AAAGCTGCTG AGACAACAGG AGCATGGATC CTTACAGAGG GCATTAATAC TGGGGCCTCC 
AAACATGTGG GTGATGCTTT AAAAGCACAC GCTTCTCAAC ACCTGCGAAA AATCTGTGCA 
GTTGGCATAC CACCATGGGG AGCTATTGAG AATCAGCAGG ATCTCATTGG CAAAGACGTT 
GTGTGCCTGT ATCAGACACT GATAAATCCA ATGAGCAAAC TGACAAGTCT CAATAGCATG 
CACTCCCATT TTATAATGGT GGATGATGGC ACAGTTGGAA AGTATGGTAA TGAAATGAAA 
TTAAGGAGAA ACTTGGAAGA CTTCATTTCC CTTCAGAAGA TCCATACCCG GATGGGGCAA 
GGAGTGCCTG TGGTAGGGCT TGTTATAGAA GGTGGTCCAA ATGTCATCTT GATGGTATGG 
GAATATGTGA GGAGCACTCC ACCAGTACCA GTTGTAGTGT GTGAAGGAAC AGGCAGAGCA 
GCTGATATAT TGGCATTTAC TCACAAGCAC ACAACGGACA CAGAGCAGAT TAGTCCACAG 
CTGAAAGAAG AAATCTTAGA GATGATTCAG AAGACATTTA ATCTTGGCCA CAGACAATCA 
AACCATGTTC TTTATATTCT AATGGAGTGT ATGGAGCGCC GTGCGTCTAT TACTATATTT 
GATGCAGAAT CTGAAGAACA GCAAGATATT GATTTGGCTA TATTGACAGC TCTGCTAAAA 
GGGACTAATA TGTCTGCTCA AGACCAGCTG AGCTTGGCGC TAACCTGGAA CAGGGTTGAC 
ATTGCCAAAA AACACATACT TATATATGGA CAACATTGGA AGGTGGGGTC TTTGGAACAA 
GCAATGCAAG ATGCACTTGT AATGGATCGG GTGGATTTTG TAAAGTTGCT AATTGAATAT 
GGTGTGAATA TGCATCGCTT CTTGACCATT GGTCATTTGG AAGAATTATA CAATACTAAA 
CAAGGCCCCA CAAATCTGCT CCTACACCAT TTAGTCAGAG ATGTAAAACA GAGCAATCCT 
TCTCCAGATT ACAGAATTTC CCTTATAGAT GTGGGACTTG TAATCGAGTA CTTGATTGGT 
GGAGCCTACC GAAGCAGTTA TACAAGGAAA AGTTTCAGAG TCCTGTACAA CAGTCTCTTC 
AGGAAGCATA AGAAATCTGT GCCCAGTTCC TCCAGCAGCC TTGCACAAAG TTTATCTCAT 
CTTTCCATAA ACCAGAGCAG CCAGATGAGC AGCAAGCTTG AATCGCCAGA GAGTACCTTG 
CATTCCCAAT TTTTCCGAAC AGCCCAACCT TGTAGACGCA AGGACAAGAC AACATGCCAT 
CAAAAGTCAA AAAAGAAAGA TGAAGTAACT TGCACAGAAG ATAATGAACC AGTTGGCTTT 
ATCTACCCTT ACAATGACCT CTTGGTTTGG GCTGTACTGA TGAAAAGACA GAAGATGGCT 
ATGTTTTTTT GGCAACATGG AGAGGAGGCC ATGGTCAAAG CAGTAATTGC CTGTAAGCTA 
TATAGGGCCA TGGCACATGA AGCTAAGCAG AGCACCATGG TGGATGACAC TTCAGAAGAA 
TTAAAGAAAT ATTCAAAAGA ATTTGGACAG CTTGCTTTGG ACATACTTGA AAATGCTTTC 
AAAATGAATG AACAAATGGC CATGAAACTT TTAACATACC AGTTAAAGAA CTGGAGTAAT 
TCAACGTGCC TGAAATTAGC GGTTTCATCA ACATTGCGGC CCTTTGTATC CCATACATGC 
ACACAGATGT TACTGACAGA CATGTGGATG GGTCGCCTGA AGATGCGGAA ACACTCCTGG 
CTTAAAGTCA TTCTAGGAAT ATTGCTGCCA CCCTTTGTCT TGATGTTGGA ATTTAAGACC 
AAGGCAGAAA TGTCCCATGT ACCCCAGTCA CTAGAGTTCC ATCAGTTCTC ACGGCTGTAC 
GTTGACCCTC CATCACTGAG CACCAAAGAG GAACATATTA CAGATTTTGA TGCTGAAAGG 
GGATCAAAGC AGCCAGGAGA AGATCAAAAT CAGAAGATCA GTAATGGTCT AGGAATCCTT 
CCGATAACCA GGAAAATTTG TGAATTTTAT AGCGCTCCCA TTGTCAAGTT CTGGTTCCAC 
ACTATGGTGT ACTTGGCATT CCTCATGCTT TTCACATACA CGGTGCTGGT AAAGATGGAC 
CCACAGCCCA GTGTCCAAGA GTGGTGTGTA ATAATTTATA TATTCACTAC GGCAGTTGAA 
AAAGTCAGGG AGGTATTTGT GTCAGAACCT GGAAAGTTCA GTCAAAAAGT AAAAGTTTGG 
ATCAATGAGT ACTGGAATCT TACAGATTCT ATTGCAATTT TCTTGTTTTT TATTGGTTTC 
GGCTTGCGCT GGCCCGACCC TCCGTTTCAA ACAGCAGGGC GCATAATATA TTGCCTGGAC 
ATAATATTTT GGTATGTGCG ACTTTTAGAC TTCTTTGCAG TGAACCAGCA AGCAGGCCCT 
TACATTACAA TGCTTGGAAA AATGACATCC AACATGTTTG GTATTGTGAT TATAATGGCA 
ATTGTTCTGT TGGCATTTGG GGTGTCCCGG AAAGCAATTC TGTCTCCCGA TCACCCTCCT 
TCTTGGACTC TTGCTCGTGA TATAGTGTTT CAGCCATACT GGATGATGTT TGGGGAAGTA 
TATGCTGGGG AAATTGATGC CTGTGAAGAA GATCCAGATT GCCCTCCTGG TTCCTTCATC 
ACCCCATTCC TTCAAGCTGT GTATCTCTTT GTGCAGTATA TCATCATGGT CAACCTGCTT 
ATTTCTCTGT TTAATAATGT CTACAGGGTA TTAAACTCTG TCTCTCACAA ACTTTGGAAA 
TACAATCGAT ATCGATATAT AATGACATAC TACGAGAAGC CATGGCTACC TCCACCCCTG 
ATATTCCTTA GTCATATATA TATACTTATC AATCGCCTTT GTCATCACCG ATCTCACAGT 
GAGTTTGACC CAGAGGAAAC TGACATGGGA CTGAAGCTGT ACCTTAGTGA TAATGAATTG 
AGGAAACTCC ATGATTTTGA GGAAAAATGT GTGGAAACAT ACTTCCATGA GAAAAATGAA 
AGCCTGCACT CCAGCAAAAA TGAAAGAATC CGTGTAACAA CAGAGAGAAC TGAAGAGATG 
TTTGCCCAAC TGAAAGAAGT TAATGATAAA GTTTTCTACA TCAAAGACTC CTTGAGCTCC 
CTGGACAGCC AATTAGGGCA TTTGCAGGAT CTCTCCGCAT TGACTGTGGA TACTTTAAAA 
ATCATTTCAG CTGTGGATTC TTTGCAAGTT GAAGAAGCCT TTATAAAAGA CAAGAAAGGA 
CTCACCTGTA AAAAGCTCCC ACACAGTTGG AGCAACGTTC CATATTCTCA TCACCAGAGA 
GAGTATAATT ACTACAGTAT GCCACCTTCA CTTCTCCGGA CCTTGGCCAG GAGTCAGTGC 
CCATCACTGT ATCAATGTCC ACCACAGCAA CTGACTGCAC AGTTAGAAAT CCATAACTCA 
GAAGAACCCA AGATAGAGGT TGATATTGTT ACTATGGCAG TGCCATTGGA TAGTAAATCA 
GCTCAGCATT ACGGCAATTA CTTGCTTGTA CCTTCAGTGG GGCCCCCTGC ACCAACTGAT 
TCTGCCTTTA AAGAGACATG TTCAGATATG CTCAGTGAAG GGGAAGCAAT ATCTGTACAA 
GCTACAACCA AAGAGAGGAT ATGTTCCATT CATTCTGACC AGTGTCTCTG TAGCAAACAA 
AAGCATGTGA AGGAAAGTAG TCGCAAGACC ATGAAAGAGG ATTTAAAGCC TCTTGTGACA 
AATACTGATG AAAGCCAAAA TGAAGAAAGC CATGAATGCT CTTGCGATCA GTTTACTGCT 
CATGTCACTG TTGAGACCTC AATTACGTGC AACATTATTG ACCCCGGAGG TGGTTATGTA 
AACTTGGCAT TTTCAGAAGA AGGAGAAACT GCCCTGTTTA GTGGAGGGAG TAGCAAGACC 
CTTTGCTCAC GTCCCACCCA AAGTTCAAAA TCTAATGTGT TTCCAAATAA GTCTGATGGA 
TCTAGAGCCA ATGATAGGCC TGAGTTTGGT TCAGGTTACA AACAGAAAAC TTCATTCCTG 
AAAAATTCAC TTTATAGGAA TAACTCACCA AGAGGCAGTT CTAAGAGGCA TAAAGAGAAG 
AAAGATGGAA AGATATGTAC AATCAAAGAT TTTGCCAAGC CTCCTGAGAG CTCTAAGGTT 
ACATGGGCCA AAGAGAAACT GCGTAGCACA TTCAGAAAGT CTGTTAAAGA CAAGCCAAGG 
CCAACTCAAC TTCAAGTTCC AGTGATAACT GTGGATGGTT GCTCTCAGAG TACGCAGATG 
AGTTCCGAAC CTGCAGATTG TGGCAGCTGG AAATCTGAAG ATGAACCCAG TAAGAACAGG 
CTTACAGTAT CAAGTTTCAA TCAGCTAAGC TTGGATCACA TGACTTACAT GCGACAGCAA 
CTAAAGACGC AAGAATTTGA AAGACACCTA GTTCAGACTA GTGAATATCT AAGGCAGTCC 
AGAGAGGATT TGCATAACAC CACCCAACTG AGAAGGAGCT CATTATTCCG AAATTCCGAT 
GAAGTAAAGA AAATGGCAGC TTCCCTAAAA GCCTCTCAGG ATTTAAGTAA TCATTATTCA 
GCGGTGGAAA GGAATAATTT AATGAGGCTT GCACAGAGCA TACCTTTCAC CCCCCTGCAC 
CTTTTTGCTG GAGAAGAAGT CACTGTTTAC CAACTAGAGG AAAGTTCTCC AGCAATTCTA 
GATAAAAGTA TGTCTTCCTG GTCACAGCGT GGAATGGCAG CAATGCTTCA GGTTCTGTCC 
CCAGAGGAAA TGGATGGAGG TCTCCGCAGG GCGTTAAAAG TTGTGTGCAC TTGGTCTGAG 
CATGATGTAC TGACGCCTGG TGAGGTGTTT ATAGTCAAAT CTTTTCTTCC GGAGGTAGTA 
CAGACTTGGC AGAAGGTTTT CCGTGGTGAT ACTGTACTGA ATCTGTGTCT CAGGGAAATT 
CAGCAACAAA GAGCAGCTCA AAAGTTGATC TACACCTTCA ATCAAGTGAA ACCACACAGT 
ATTCCTTATA CTCTAAGGTT TCTAGAAGTA TTTCTTATAT TCTGCCATTC AGCTAACCAA 
TGGCTTACTA TAGAGAAGTA CCTGACTGGA GAATTCCGCA AGTACAATAA CAACAATGGA 
GATGAAATTA CCCCCACCAC TTTACTTGAG GAGACACTGC TGTCTTTTTC TCATTGGAGT 
TATGAATATA CAAGGGGAGA GCTGCTTGTT TTGGATTTAC AAGGCGTTGG GGAAAATCTT 
ACAGACCCAT CTGTTATTAA ACCAGAAGAC AAACAATCAA GAGGAATGGT ATTTGGACCA 
GCTAATTTAG GAGAAGATGC TCTGACTAAT TATGTTTCAA AGCATCGCTG CAATTCCTGC 
TGCAGAAAGC TTAAACTCCT TGATTTGAAA AGAAATGACT ACTCACCTGG GAGGATCAAT 
CTAGGGTTTG AAATTGGAGA AGCAGACACA GATAAAGACC TTGTGGAATA TAATACAAGA 
CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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trpm6 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

オンラインフォーム

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