usp40 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	usp40
Entrez Gene ID	100486255
Full Name	ubiquitin specific peptidase 40
General protein information	Preferred Names ubiquitin specific peptidase 40 Names ubiquitin carboxyl-terminal hydrolase 40
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002932005.4	XP_002932051.2	ubiquitin carboxyl-terminal hydrolase 40
XM_002932005.5	XP_002932051.3	ubiquitin carboxyl-terminal hydrolase 40
XM_012971227.2	XP_012826681.1	ubiquitin carboxyl-terminal hydrolase 40
XM_012971226.2	XP_012826680.1	ubiquitin carboxyl-terminal hydrolase 40
XM_012971227.3	XP_012826681.2	ubiquitin carboxyl-terminal hydrolase 40
XM_012971226.3	XP_012826680.2	ubiquitin carboxyl-terminal hydrolase 40

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Usp40	NP_001185502.1
Gallus gallus (chicken)	USP40	NP_001244247.1
Danio rerio (zebrafish)	usp40	XP_001921353.3
Xenopus tropicalis (tropical clawed frog)	usp40	XP_002932051.2
Homo sapiens (human)	USP40	NP_060688.1
Bos taurus (cattle)	USP40	XP_002686602.2
	USP40	XP_001110228.1
Rattus norvegicus (Norway rat)	Usp40	NP_001128357.1
Pan troglodytes (chimpanzee)	USP40	XP_516162.3

Gene Ontology

The following usp40 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the usp40 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa66235	XM_002932005.5 Latest version!	Xenopus tropicalis ubiquitin specific peptidase 40 (usp40), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa66235	XM_012971227.3 Latest version!	Xenopus tropicalis ubiquitin specific peptidase 40 (usp40), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa66235	XM_012971226.3 Latest version!	Xenopus tropicalis ubiquitin specific peptidase 40 (usp40), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa16185	XM_002932005.4 Latest version!	Xenopus tropicalis ubiquitin specific peptidase 40 (usp40), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa16185	XM_012971226.2 Latest version!	Xenopus tropicalis ubiquitin specific peptidase 40 (usp40), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa16185	XM_012971227.2 Latest version!	Xenopus tropicalis ubiquitin specific peptidase 40 (usp40), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

usp40 ( XM_002932005.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002932051.3 Xenopus tropicalis ubiquitin specific peptidase 40 (usp40), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa66235
Clone ID Related Accession (Same CDS sequence)	XM_002932005.5 , XM_012971227.3 , XM_012971226.3
Accession Version	XM_002932005.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ubiquitin carboxyl-terminal hydrolase 40
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002932005.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGGAG ACCTATTTGA GGAAGATGAA GACTTTTCAT TCCTTTTGAG CAGTCCAAAC 
AAGAATGGCA AGAAATCCCA CCCCAGAGAT TCAAAGACCC CAGAGCCCCG AGGAGACACA 
AGGCTTAGTG GACTGAGGAA TCAAGGTGGC ACCTGTTACC TAAATTCTCT TCTGCAGACC 
CTGTTTTTCA CACCAGAATT CAGAGAAGCT CTTTTTGCTT TGGGTCCTAA AGAACTTGGA 
TCACTGGAGG AGAAAGACAC GCCAGACTCT CGGGTGCGAA TTATTCCCCT GCAGTTACAG 
CGTCTCTTCG CTCAACTTCT GCTGCTGGAT CAGCAGGCCT TGTCAACCAC AGATCTCACA 
GAGAGCTTTG GGTGGAACAG TAGTGAGGAG ACAAGCCAGC ATGATGTGCA AGAGTTAAAC 
CGTATTTTGT TCAGTGCTCT TGAGAGCTCA TTGGAGGGCA CATCTGGCCA TGACCTCATC 
AAGCGATTGT ACCATGGCAC CATTGTTAAC TGTATCCAGT GCAAGGAGTG TGGCTACATC 
AGTGAAAGGC AGGAGGATTT CCTTGACTTG ACGGTGGCAG TGAAGGGCGT GGGCAGTCTG 
GAGAATTCTC TGTGTAGCAT GTATTTGGAA GAGGAACACT TTGATGGAGA TAATTTGTAC 
AGATGTGGGA GCTGTGCCAA GCTTGTCCCT GCAACCAAGT CTGCCAAGTT GGGGAAACTG 
CCACCCTTTC TCACACTATC TCTGCTGAGG TTTAACTTTG ACTTTGTAAA GTGTGAGCGT 
TATAAGGAAA CTTCACGATA CACCTTTCCC ACCCGCCTCA TCATCCGGCC CTTTTGTGAG 
CAGCATAATC TAGAAGACTC CGTATATGCC TATGAGCTCT TCTCTGTTAT TATACACAAG 
GGCGGCTGTT ATGGAGGTCA TTATCATGCA TATATTCAAG ATGTCGATGA GCTGGGGCAT 
TGGGGACTCA AGGAAGTGGC TAAAAAGACA GCATCAGCAC AGGAGTCTGA GAGTAAACAA 
GATTGCCCAA TAGTTGCTCT TGCTTCCATC ATAGCAGAGG CTGGGGCCGA TCAAATGGTG 
GCAGTTGACC AGCTGGGACA GAAGCTATTG GAATACATGG GAACATCTTG GAATAAGACT 
TATCGCAAGC AGCATGGAGC TATGCACAAG TTCCTGCAAA GCCATCCCTC CGTTTTCCAG 
ATGAGTGCAG ATGGCTCGCT TGTAGGTTTG GCGCATAGTG TTCCAGACAC AGTAGGCTCC 
GATTCAGAGA GCTCTAGGCC AGGTACTCCA GAGAAATCTC AAGCTCCATC TAGAAGTGCT 
GGTCATTGGT TTGATTTTAA CGATTCCAAA GTGGAACCCA TCCGCGAGAC GGATATTGAA 
AAGCAATTCC AGGGCAAGGA ATCTGCATAT ATGCTTTTTT ATCGCAAGTG TGAAATGAAT 
AGACCACCAG AAGCTAAAGG CAATCCTCGC TTTGGAGTCC CAGAGCACAT TCTAAATGAC 
ATGGACCAAG AAAATATCGA GCTTCAGACG AAAAGAACTG AATCGGCCTT ACAGATCAAC 
TGCATGGACC TTTGTCTCCA TCTGTCTTCT CATTACCAAT ATGAAAATGG TGCCCTGCAT 
CCAGCACATC CCCGAAACAA CAGTAAGCTG GCAATTGCTA TAGACCGCAG GAGGACTGTA 
GAAGATCTAA AACAATTAGC TTTCCAGGTT CTTGATGAAT CTTTGGAAGG ACAAGTCCTG 
AGTATTGCTA GAGTTCTACC TGCTGGACTT CATATCTTTC AGAATCTGAT TGATGCCAAG 
ATGGATCTGG TCACTGCAGG AATTACAGAT GGAACTGACA TCTTTGTGTG GGATGGAAAG 
CAGGTTGGGG GTGTAATGAT TCAGAGTGGA GCAGAGTTCA CGCCAGTCCT GCTCACCATC 
CTGCGCCCAG TCCTGCAAAC AAATTCTTCA GCTGAGTTCA GTGAGAGTCA GAGAGCGTTC 
CCATCATGTG CCTGCTTGAA AGACATACAA AAAGAGCTTG CTGGCCCCAC CTGTGAGGAG 
TCAGTGTTGT GCTTCCCTTC GTCAGAGAAT AGTGAGCAGT GGAGTTTTTA TTCTGCTGAC 
GACTCAAACA AGAGCCTAAA AGAATTGGGG CTAAGAGATG GCAGCTCTAT TCTCTTGTTT 
GATACACGTT CGGATGGGGA TGTATATCTT GCATCAGAAA GACATTTATC TAAAGACGCG 
AAGCAGAACT TCCTCATGGT GCAGGACTGG TGCAGACAGC AGGATGAGAA AGAAACGGTT 
AAAATAATTA TCACTCCCAA CACTACTATA CAAGATATCC ATGTGAAAGC AGTCGAATTG 
CTGCAGCTAC AGGAAGAGTG TAGCAAAAAT GACACTTGCT TAAGACCCCT CCACAGAAAA 
GAGAAGCTTC TTCCCCCAGT GCCAGAAAGC CTAACTGTGC AGGAGGCAGA ACTGAAAAAT 
GGATGTACCT TAGGGCTCTG CAAAGGGAAA GCCCCCAGCC CCTCCCAGAT CTTCTTGTAT 
TTCATTTCCG GGACTGACCT GCAGGAGGGC AGCGAGCAGG AGATTATTTT AGAAGAAACT 
CTCACTGTGA GACAGAGCCT GGAAAGGATG CTGAATAAAG CAGGTTTGCC AGGTAAGGCA 
GGATGGCACC TGAGAAAGAT GGACTGGTGT TATGAAGCAG GAGAGGCTCT GAGCGATGAG 
GAGTCCACAC TGAAAGAGCT GAATATCTGC GCTGGAGACA CATTGGTGAT CACTGAAGGG 
AAACTTCCAC CCAAGGGTTT CCTGAAGCTT TCTATATGGC TGTACTGCCC CCATACACAG 
CAAGATGCTA TGAATCATGT GACCTGTGAA CCAGGTGCTC TACAGACCTC CACTACAGAA 
GATAATAACA TGCAAGAAGG TGGAACCTCT AATTTCTGCT CTGTTGGTGA GATAGAAATA 
TCAGGAGACT TATCATTACA GGATGTGAAA ATGCAGATCC TGACTTTGCC AGTTTTTGAA 
GCCCTAGGGA TCCCTTCTCC TGAGTTCCTC AGAGTCTGGA CCCTGGAAAA CAAGCATCTG 
GGGAAAATCC TGCGCTCGCC TCCAGTGTTA CTCAGTGAGT ACAAACTGGG GAGCAAGCCA 
GCACTATGCA TTGAGCCTTT GCAGGTGGAG GAATGTTTGG GTGCTAAAGA TCTTGTCTTG 
CGGGTGCAGC TGTCTGTCCC AGGGGAGCGT CGCTATTTTC CTCCCGTAGA TATTGTGTGG 
GATATATCCA GAGGCTGCAC AGCCCCAGCC TTGCTGCACA GAATTGCCAT GCATTGCTCC 
CTGCCAACAG ACAAGGTGGA AATTGCAAAG CACTTTCCAG AGAAATATGA GTGGCTTCCA 
ATATCCAGTT GGACCCAACA TGTCTCCAGA AAGAGGAAAA AGAAAAAGCT GGAAAGTCTA 
CAGGGGGCTC CTTATCACCT AAAGGATGGG GACATCATTG GGGTCAAGAA TCTTTTGCTT 
GATGACTGGA CAGACTTCAG CTCTGAAATT GACCGCATTG GAAAAGAAAA GCAGCAGCAG 
CAGCAGCGCC CAGCGAATGG AAACAAAAGT CTGCACGCAG AAGGATTGCA GAATAATACG 
GCTGGAAGCA ATGGAAGGGC AAAGTCGAAG GCCCGGAAAG CGGAGACCTC TCTCTCAATC 
CGAGTGGGCG TATTCAGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002932051.3
CDS	980..4660
Translation	MFGDLFEEDEDFSFLLSSPNKNGKKSHPRDSKTPEPRGDTRLSGLRNQGGTCYLNSLLQTLFFTPEFREALFALGPKELGSLEEKDTPDSRVRIIPLQLQRLFAQLLLLDQQALSTTDLTESFGWNSSEETSQHDVQELNRILFSALESSLEGTSGHDLIKRLYHGTIVNCIQCKECGYISERQEDFLDLTVAVKGVGSLENSLCSMYLEEEHFDGDNLYRCGSCAKLVPATKSAKLGKLPPFLTLSLLRFNFDFVKCERYKETSRYTFPTRLIIRPFCEQHNLEDSVYAYELFSVIIHKGGCYGGHYHAYIQDVDELGHWGLKEVAKKTASAQESESKQDCPIVALASIIAEAGADQMVAVDQLGQKLLEYMGTSWNKTYRKQHGAMHKFLQSHPSVFQMSADGSLVGLAHSVPDTVGSDSESSRPGTPEKSQAPSRSAGHWFDFNDSKVEPIRETDIEKQFQGKESAYMLFYRKCEMNRPPEAKGNPRFGVPEHILNDMDQENIELQTKRTESALQINCMDLCLHLSSHYQYENGALHPAHPRNNSKLAIAIDRRRTVEDLKQLAFQVLDESLEGQVLSIARVLPAGLHIFQNLIDAKMDLVTAGITDGTDIFVWDGKQVGGVMIQSGAEFTPVLLTILRPVLQTNSSAEFSESQRAFPSCACLKDIQKELAGPTCEESVLCFPSSENSEQWSFYSADDSNKSLKELGLRDGSSILLFDTRSDGDVYLASERHLSKDAKQNFLMVQDWCRQQDEKETVKIIITPNTTIQDIHVKAVELLQLQEECSKNDTCLRPLHRKEKLLPPVPESLTVQEAELKNGCTLGLCKGKAPSPSQIFLYFISGTDLQEGSEQEIILEETLTVRQSLERMLNKAGLPGKAGWHLRKMDWCYEAGEALSDEESTLKELNICAGDTLVITEGKLPPKGFLKLSIWLYCPHTQQDAMNHVTCEPGALQTSTTEDNNMQEGGTSNFCSVGEIEISGDLSLQDVKMQILTLPVFEALGIPSPEFLRVWTLENKHLGKILRSPPVLLSEYKLGSKPALCIEPLQVEECLGAKDLVLRVQLSVPGERRYFPPVDIVWDISRGCTAPALLHRIAMHCSLPTDKVEIAKHFPEKYEWLPISSWTQHVSRKRKKKKLESLQGAPYHLKDGDIIGVKNLLLDDWTDFSSEIDRIGKEKQQQQQRPANGNKSLHAEGLQNNTAGSNGRAKSKARKAETSLSIRVGVFR

Target ORF information:

RefSeq Version	XM_002932005.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin specific peptidase 40 (usp40), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002932005.5

ORF Insert Sequence:

ATGTTTGGAG ACCTATTTGA GGAAGATGAA GACTTTTCAT TCCTTTTGAG CAGTCCAAAC 
AAGAATGGCA AGAAATCCCA CCCCAGAGAT TCAAAGACCC CAGAGCCCCG AGGAGACACA 
AGGCTTAGTG GACTGAGGAA TCAAGGTGGC ACCTGTTACC TAAATTCTCT TCTGCAGACC 
CTGTTTTTCA CACCAGAATT CAGAGAAGCT CTTTTTGCTT TGGGTCCTAA AGAACTTGGA 
TCACTGGAGG AGAAAGACAC GCCAGACTCT CGGGTGCGAA TTATTCCCCT GCAGTTACAG 
CGTCTCTTCG CTCAACTTCT GCTGCTGGAT CAGCAGGCCT TGTCAACCAC AGATCTCACA 
GAGAGCTTTG GGTGGAACAG TAGTGAGGAG ACAAGCCAGC ATGATGTGCA AGAGTTAAAC 
CGTATTTTGT TCAGTGCTCT TGAGAGCTCA TTGGAGGGCA CATCTGGCCA TGACCTCATC 
AAGCGATTGT ACCATGGCAC CATTGTTAAC TGTATCCAGT GCAAGGAGTG TGGCTACATC 
AGTGAAAGGC AGGAGGATTT CCTTGACTTG ACGGTGGCAG TGAAGGGCGT GGGCAGTCTG 
GAGAATTCTC TGTGTAGCAT GTATTTGGAA GAGGAACACT TTGATGGAGA TAATTTGTAC 
AGATGTGGGA GCTGTGCCAA GCTTGTCCCT GCAACCAAGT CTGCCAAGTT GGGGAAACTG 
CCACCCTTTC TCACACTATC TCTGCTGAGG TTTAACTTTG ACTTTGTAAA GTGTGAGCGT 
TATAAGGAAA CTTCACGATA CACCTTTCCC ACCCGCCTCA TCATCCGGCC CTTTTGTGAG 
CAGCATAATC TAGAAGACTC CGTATATGCC TATGAGCTCT TCTCTGTTAT TATACACAAG 
GGCGGCTGTT ATGGAGGTCA TTATCATGCA TATATTCAAG ATGTCGATGA GCTGGGGCAT 
TGGGGACTCA AGGAAGTGGC TAAAAAGACA GCATCAGCAC AGGAGTCTGA GAGTAAACAA 
GATTGCCCAA TAGTTGCTCT TGCTTCCATC ATAGCAGAGG CTGGGGCCGA TCAAATGGTG 
GCAGTTGACC AGCTGGGACA GAAGCTATTG GAATACATGG GAACATCTTG GAATAAGACT 
TATCGCAAGC AGCATGGAGC TATGCACAAG TTCCTGCAAA GCCATCCCTC CGTTTTCCAG 
ATGAGTGCAG ATGGCTCGCT TGTAGGTTTG GCGCATAGTG TTCCAGACAC AGTAGGCTCC 
GATTCAGAGA GCTCTAGGCC AGGTACTCCA GAGAAATCTC AAGCTCCATC TAGAAGTGCT 
GGTCATTGGT TTGATTTTAA CGATTCCAAA GTGGAACCCA TCCGCGAGAC GGATATTGAA 
AAGCAATTCC AGGGCAAGGA ATCTGCATAT ATGCTTTTTT ATCGCAAGTG TGAAATGAAT 
AGACCACCAG AAGCTAAAGG CAATCCTCGC TTTGGAGTCC CAGAGCACAT TCTAAATGAC 
ATGGACCAAG AAAATATCGA GCTTCAGACG AAAAGAACTG AATCGGCCTT ACAGATCAAC 
TGCATGGACC TTTGTCTCCA TCTGTCTTCT CATTACCAAT ATGAAAATGG TGCCCTGCAT 
CCAGCACATC CCCGAAACAA CAGTAAGCTG GCAATTGCTA TAGACCGCAG GAGGACTGTA 
GAAGATCTAA AACAATTAGC TTTCCAGGTT CTTGATGAAT CTTTGGAAGG ACAAGTCCTG 
AGTATTGCTA GAGTTCTACC TGCTGGACTT CATATCTTTC AGAATCTGAT TGATGCCAAG 
ATGGATCTGG TCACTGCAGG AATTACAGAT GGAACTGACA TCTTTGTGTG GGATGGAAAG 
CAGGTTGGGG GTGTAATGAT TCAGAGTGGA GCAGAGTTCA CGCCAGTCCT GCTCACCATC 
CTGCGCCCAG TCCTGCAAAC AAATTCTTCA GCTGAGTTCA GTGAGAGTCA GAGAGCGTTC 
CCATCATGTG CCTGCTTGAA AGACATACAA AAAGAGCTTG CTGGCCCCAC CTGTGAGGAG 
TCAGTGTTGT GCTTCCCTTC GTCAGAGAAT AGTGAGCAGT GGAGTTTTTA TTCTGCTGAC 
GACTCAAACA AGAGCCTAAA AGAATTGGGG CTAAGAGATG GCAGCTCTAT TCTCTTGTTT 
GATACACGTT CGGATGGGGA TGTATATCTT GCATCAGAAA GACATTTATC TAAAGACGCG 
AAGCAGAACT TCCTCATGGT GCAGGACTGG TGCAGACAGC AGGATGAGAA AGAAACGGTT 
AAAATAATTA TCACTCCCAA CACTACTATA CAAGATATCC ATGTGAAAGC AGTCGAATTG 
CTGCAGCTAC AGGAAGAGTG TAGCAAAAAT GACACTTGCT TAAGACCCCT CCACAGAAAA 
GAGAAGCTTC TTCCCCCAGT GCCAGAAAGC CTAACTGTGC AGGAGGCAGA ACTGAAAAAT 
GGATGTACCT TAGGGCTCTG CAAAGGGAAA GCCCCCAGCC CCTCCCAGAT CTTCTTGTAT 
TTCATTTCCG GGACTGACCT GCAGGAGGGC AGCGAGCAGG AGATTATTTT AGAAGAAACT 
CTCACTGTGA GACAGAGCCT GGAAAGGATG CTGAATAAAG CAGGTTTGCC AGGTAAGGCA 
GGATGGCACC TGAGAAAGAT GGACTGGTGT TATGAAGCAG GAGAGGCTCT GAGCGATGAG 
GAGTCCACAC TGAAAGAGCT GAATATCTGC GCTGGAGACA CATTGGTGAT CACTGAAGGG 
AAACTTCCAC CCAAGGGTTT CCTGAAGCTT TCTATATGGC TGTACTGCCC CCATACACAG 
CAAGATGCTA TGAATCATGT GACCTGTGAA CCAGGTGCTC TACAGACCTC CACTACAGAA 
GATAATAACA TGCAAGAAGG TGGAACCTCT AATTTCTGCT CTGTTGGTGA GATAGAAATA 
TCAGGAGACT TATCATTACA GGATGTGAAA ATGCAGATCC TGACTTTGCC AGTTTTTGAA 
GCCCTAGGGA TCCCTTCTCC TGAGTTCCTC AGAGTCTGGA CCCTGGAAAA CAAGCATCTG 
GGGAAAATCC TGCGCTCGCC TCCAGTGTTA CTCAGTGAGT ACAAACTGGG GAGCAAGCCA 
GCACTATGCA TTGAGCCTTT GCAGGTGGAG GAATGTTTGG GTGCTAAAGA TCTTGTCTTG 
CGGGTGCAGC TGTCTGTCCC AGGGGAGCGT CGCTATTTTC CTCCCGTAGA TATTGTGTGG 
GATATATCCA GAGGCTGCAC AGCCCCAGCC TTGCTGCACA GAATTGCCAT GCATTGCTCC 
CTGCCAACAG ACAAGGTGGA AATTGCAAAG CACTTTCCAG AGAAATATGA GTGGCTTCCA 
ATATCCAGTT GGACCCAACA TGTCTCCAGA AAGAGGAAAA AGAAAAAGCT GGAAAGTCTA 
CAGGGGGCTC CTTATCACCT AAAGGATGGG GACATCATTG GGGTCAAGAA TCTTTTGCTT 
GATGACTGGA CAGACTTCAG CTCTGAAATT GACCGCATTG GAAAAGAAAA GCAGCAGCAG 
CAGCAGCGCC CAGCGAATGG AAACAAAAGT CTGCACGCAG AAGGATTGCA GAATAATACG 
GCTGGAAGCA ATGGAAGGGC AAAGTCGAAG GCCCGGAAAG CGGAGACCTC TCTCTCAATC 
CGAGTGGGCG TATTCAGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp40 ( XM_012971227.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826681.2 Xenopus tropicalis ubiquitin specific peptidase 40 (usp40), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa66235
Clone ID Related Accession (Same CDS sequence)	XM_002932005.5 , XM_012971227.3 , XM_012971226.3
Accession Version	XM_012971227.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ubiquitin carboxyl-terminal hydrolase 40
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012971227.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGGAG ACCTATTTGA GGAAGATGAA GACTTTTCAT TCCTTTTGAG CAGTCCAAAC 
AAGAATGGCA AGAAATCCCA CCCCAGAGAT TCAAAGACCC CAGAGCCCCG AGGAGACACA 
AGGCTTAGTG GACTGAGGAA TCAAGGTGGC ACCTGTTACC TAAATTCTCT TCTGCAGACC 
CTGTTTTTCA CACCAGAATT CAGAGAAGCT CTTTTTGCTT TGGGTCCTAA AGAACTTGGA 
TCACTGGAGG AGAAAGACAC GCCAGACTCT CGGGTGCGAA TTATTCCCCT GCAGTTACAG 
CGTCTCTTCG CTCAACTTCT GCTGCTGGAT CAGCAGGCCT TGTCAACCAC AGATCTCACA 
GAGAGCTTTG GGTGGAACAG TAGTGAGGAG ACAAGCCAGC ATGATGTGCA AGAGTTAAAC 
CGTATTTTGT TCAGTGCTCT TGAGAGCTCA TTGGAGGGCA CATCTGGCCA TGACCTCATC 
AAGCGATTGT ACCATGGCAC CATTGTTAAC TGTATCCAGT GCAAGGAGTG TGGCTACATC 
AGTGAAAGGC AGGAGGATTT CCTTGACTTG ACGGTGGCAG TGAAGGGCGT GGGCAGTCTG 
GAGAATTCTC TGTGTAGCAT GTATTTGGAA GAGGAACACT TTGATGGAGA TAATTTGTAC 
AGATGTGGGA GCTGTGCCAA GCTTGTCCCT GCAACCAAGT CTGCCAAGTT GGGGAAACTG 
CCACCCTTTC TCACACTATC TCTGCTGAGG TTTAACTTTG ACTTTGTAAA GTGTGAGCGT 
TATAAGGAAA CTTCACGATA CACCTTTCCC ACCCGCCTCA TCATCCGGCC CTTTTGTGAG 
CAGCATAATC TAGAAGACTC CGTATATGCC TATGAGCTCT TCTCTGTTAT TATACACAAG 
GGCGGCTGTT ATGGAGGTCA TTATCATGCA TATATTCAAG ATGTCGATGA GCTGGGGCAT 
TGGGGACTCA AGGAAGTGGC TAAAAAGACA GCATCAGCAC AGGAGTCTGA GAGTAAACAA 
GATTGCCCAA TAGTTGCTCT TGCTTCCATC ATAGCAGAGG CTGGGGCCGA TCAAATGGTG 
GCAGTTGACC AGCTGGGACA GAAGCTATTG GAATACATGG GAACATCTTG GAATAAGACT 
TATCGCAAGC AGCATGGAGC TATGCACAAG TTCCTGCAAA GCCATCCCTC CGTTTTCCAG 
ATGAGTGCAG ATGGCTCGCT TGTAGGTTTG GCGCATAGTG TTCCAGACAC AGTAGGCTCC 
GATTCAGAGA GCTCTAGGCC AGGTACTCCA GAGAAATCTC AAGCTCCATC TAGAAGTGCT 
GGTCATTGGT TTGATTTTAA CGATTCCAAA GTGGAACCCA TCCGCGAGAC GGATATTGAA 
AAGCAATTCC AGGGCAAGGA ATCTGCATAT ATGCTTTTTT ATCGCAAGTG TGAAATGAAT 
AGACCACCAG AAGCTAAAGG CAATCCTCGC TTTGGAGTCC CAGAGCACAT TCTAAATGAC 
ATGGACCAAG AAAATATCGA GCTTCAGACG AAAAGAACTG AATCGGCCTT ACAGATCAAC 
TGCATGGACC TTTGTCTCCA TCTGTCTTCT CATTACCAAT ATGAAAATGG TGCCCTGCAT 
CCAGCACATC CCCGAAACAA CAGTAAGCTG GCAATTGCTA TAGACCGCAG GAGGACTGTA 
GAAGATCTAA AACAATTAGC TTTCCAGGTT CTTGATGAAT CTTTGGAAGG ACAAGTCCTG 
AGTATTGCTA GAGTTCTACC TGCTGGACTT CATATCTTTC AGAATCTGAT TGATGCCAAG 
ATGGATCTGG TCACTGCAGG AATTACAGAT GGAACTGACA TCTTTGTGTG GGATGGAAAG 
CAGGTTGGGG GTGTAATGAT TCAGAGTGGA GCAGAGTTCA CGCCAGTCCT GCTCACCATC 
CTGCGCCCAG TCCTGCAAAC AAATTCTTCA GCTGAGTTCA GTGAGAGTCA GAGAGCGTTC 
CCATCATGTG CCTGCTTGAA AGACATACAA AAAGAGCTTG CTGGCCCCAC CTGTGAGGAG 
TCAGTGTTGT GCTTCCCTTC GTCAGAGAAT AGTGAGCAGT GGAGTTTTTA TTCTGCTGAC 
GACTCAAACA AGAGCCTAAA AGAATTGGGG CTAAGAGATG GCAGCTCTAT TCTCTTGTTT 
GATACACGTT CGGATGGGGA TGTATATCTT GCATCAGAAA GACATTTATC TAAAGACGCG 
AAGCAGAACT TCCTCATGGT GCAGGACTGG TGCAGACAGC AGGATGAGAA AGAAACGGTT 
AAAATAATTA TCACTCCCAA CACTACTATA CAAGATATCC ATGTGAAAGC AGTCGAATTG 
CTGCAGCTAC AGGAAGAGTG TAGCAAAAAT GACACTTGCT TAAGACCCCT CCACAGAAAA 
GAGAAGCTTC TTCCCCCAGT GCCAGAAAGC CTAACTGTGC AGGAGGCAGA ACTGAAAAAT 
GGATGTACCT TAGGGCTCTG CAAAGGGAAA GCCCCCAGCC CCTCCCAGAT CTTCTTGTAT 
TTCATTTCCG GGACTGACCT GCAGGAGGGC AGCGAGCAGG AGATTATTTT AGAAGAAACT 
CTCACTGTGA GACAGAGCCT GGAAAGGATG CTGAATAAAG CAGGTTTGCC AGGTAAGGCA 
GGATGGCACC TGAGAAAGAT GGACTGGTGT TATGAAGCAG GAGAGGCTCT GAGCGATGAG 
GAGTCCACAC TGAAAGAGCT GAATATCTGC GCTGGAGACA CATTGGTGAT CACTGAAGGG 
AAACTTCCAC CCAAGGGTTT CCTGAAGCTT TCTATATGGC TGTACTGCCC CCATACACAG 
CAAGATGCTA TGAATCATGT GACCTGTGAA CCAGGTGCTC TACAGACCTC CACTACAGAA 
GATAATAACA TGCAAGAAGG TGGAACCTCT AATTTCTGCT CTGTTGGTGA GATAGAAATA 
TCAGGAGACT TATCATTACA GGATGTGAAA ATGCAGATCC TGACTTTGCC AGTTTTTGAA 
GCCCTAGGGA TCCCTTCTCC TGAGTTCCTC AGAGTCTGGA CCCTGGAAAA CAAGCATCTG 
GGGAAAATCC TGCGCTCGCC TCCAGTGTTA CTCAGTGAGT ACAAACTGGG GAGCAAGCCA 
GCACTATGCA TTGAGCCTTT GCAGGTGGAG GAATGTTTGG GTGCTAAAGA TCTTGTCTTG 
CGGGTGCAGC TGTCTGTCCC AGGGGAGCGT CGCTATTTTC CTCCCGTAGA TATTGTGTGG 
GATATATCCA GAGGCTGCAC AGCCCCAGCC TTGCTGCACA GAATTGCCAT GCATTGCTCC 
CTGCCAACAG ACAAGGTGGA AATTGCAAAG CACTTTCCAG AGAAATATGA GTGGCTTCCA 
ATATCCAGTT GGACCCAACA TGTCTCCAGA AAGAGGAAAA AGAAAAAGCT GGAAAGTCTA 
CAGGGGGCTC CTTATCACCT AAAGGATGGG GACATCATTG GGGTCAAGAA TCTTTTGCTT 
GATGACTGGA CAGACTTCAG CTCTGAAATT GACCGCATTG GAAAAGAAAA GCAGCAGCAG 
CAGCAGCGCC CAGCGAATGG AAACAAAAGT CTGCACGCAG AAGGATTGCA GAATAATACG 
GCTGGAAGCA ATGGAAGGGC AAAGTCGAAG GCCCGGAAAG CGGAGACCTC TCTCTCAATC 
CGAGTGGGCG TATTCAGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826681.2
CDS	294..3974
Translation	MFGDLFEEDEDFSFLLSSPNKNGKKSHPRDSKTPEPRGDTRLSGLRNQGGTCYLNSLLQTLFFTPEFREALFALGPKELGSLEEKDTPDSRVRIIPLQLQRLFAQLLLLDQQALSTTDLTESFGWNSSEETSQHDVQELNRILFSALESSLEGTSGHDLIKRLYHGTIVNCIQCKECGYISERQEDFLDLTVAVKGVGSLENSLCSMYLEEEHFDGDNLYRCGSCAKLVPATKSAKLGKLPPFLTLSLLRFNFDFVKCERYKETSRYTFPTRLIIRPFCEQHNLEDSVYAYELFSVIIHKGGCYGGHYHAYIQDVDELGHWGLKEVAKKTASAQESESKQDCPIVALASIIAEAGADQMVAVDQLGQKLLEYMGTSWNKTYRKQHGAMHKFLQSHPSVFQMSADGSLVGLAHSVPDTVGSDSESSRPGTPEKSQAPSRSAGHWFDFNDSKVEPIRETDIEKQFQGKESAYMLFYRKCEMNRPPEAKGNPRFGVPEHILNDMDQENIELQTKRTESALQINCMDLCLHLSSHYQYENGALHPAHPRNNSKLAIAIDRRRTVEDLKQLAFQVLDESLEGQVLSIARVLPAGLHIFQNLIDAKMDLVTAGITDGTDIFVWDGKQVGGVMIQSGAEFTPVLLTILRPVLQTNSSAEFSESQRAFPSCACLKDIQKELAGPTCEESVLCFPSSENSEQWSFYSADDSNKSLKELGLRDGSSILLFDTRSDGDVYLASERHLSKDAKQNFLMVQDWCRQQDEKETVKIIITPNTTIQDIHVKAVELLQLQEECSKNDTCLRPLHRKEKLLPPVPESLTVQEAELKNGCTLGLCKGKAPSPSQIFLYFISGTDLQEGSEQEIILEETLTVRQSLERMLNKAGLPGKAGWHLRKMDWCYEAGEALSDEESTLKELNICAGDTLVITEGKLPPKGFLKLSIWLYCPHTQQDAMNHVTCEPGALQTSTTEDNNMQEGGTSNFCSVGEIEISGDLSLQDVKMQILTLPVFEALGIPSPEFLRVWTLENKHLGKILRSPPVLLSEYKLGSKPALCIEPLQVEECLGAKDLVLRVQLSVPGERRYFPPVDIVWDISRGCTAPALLHRIAMHCSLPTDKVEIAKHFPEKYEWLPISSWTQHVSRKRKKKKLESLQGAPYHLKDGDIIGVKNLLLDDWTDFSSEIDRIGKEKQQQQQRPANGNKSLHAEGLQNNTAGSNGRAKSKARKAETSLSIRVGVFR

Target ORF information:

RefSeq Version	XM_012971227.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin specific peptidase 40 (usp40), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971227.3

ORF Insert Sequence:

ATGTTTGGAG ACCTATTTGA GGAAGATGAA GACTTTTCAT TCCTTTTGAG CAGTCCAAAC 
AAGAATGGCA AGAAATCCCA CCCCAGAGAT TCAAAGACCC CAGAGCCCCG AGGAGACACA 
AGGCTTAGTG GACTGAGGAA TCAAGGTGGC ACCTGTTACC TAAATTCTCT TCTGCAGACC 
CTGTTTTTCA CACCAGAATT CAGAGAAGCT CTTTTTGCTT TGGGTCCTAA AGAACTTGGA 
TCACTGGAGG AGAAAGACAC GCCAGACTCT CGGGTGCGAA TTATTCCCCT GCAGTTACAG 
CGTCTCTTCG CTCAACTTCT GCTGCTGGAT CAGCAGGCCT TGTCAACCAC AGATCTCACA 
GAGAGCTTTG GGTGGAACAG TAGTGAGGAG ACAAGCCAGC ATGATGTGCA AGAGTTAAAC 
CGTATTTTGT TCAGTGCTCT TGAGAGCTCA TTGGAGGGCA CATCTGGCCA TGACCTCATC 
AAGCGATTGT ACCATGGCAC CATTGTTAAC TGTATCCAGT GCAAGGAGTG TGGCTACATC 
AGTGAAAGGC AGGAGGATTT CCTTGACTTG ACGGTGGCAG TGAAGGGCGT GGGCAGTCTG 
GAGAATTCTC TGTGTAGCAT GTATTTGGAA GAGGAACACT TTGATGGAGA TAATTTGTAC 
AGATGTGGGA GCTGTGCCAA GCTTGTCCCT GCAACCAAGT CTGCCAAGTT GGGGAAACTG 
CCACCCTTTC TCACACTATC TCTGCTGAGG TTTAACTTTG ACTTTGTAAA GTGTGAGCGT 
TATAAGGAAA CTTCACGATA CACCTTTCCC ACCCGCCTCA TCATCCGGCC CTTTTGTGAG 
CAGCATAATC TAGAAGACTC CGTATATGCC TATGAGCTCT TCTCTGTTAT TATACACAAG 
GGCGGCTGTT ATGGAGGTCA TTATCATGCA TATATTCAAG ATGTCGATGA GCTGGGGCAT 
TGGGGACTCA AGGAAGTGGC TAAAAAGACA GCATCAGCAC AGGAGTCTGA GAGTAAACAA 
GATTGCCCAA TAGTTGCTCT TGCTTCCATC ATAGCAGAGG CTGGGGCCGA TCAAATGGTG 
GCAGTTGACC AGCTGGGACA GAAGCTATTG GAATACATGG GAACATCTTG GAATAAGACT 
TATCGCAAGC AGCATGGAGC TATGCACAAG TTCCTGCAAA GCCATCCCTC CGTTTTCCAG 
ATGAGTGCAG ATGGCTCGCT TGTAGGTTTG GCGCATAGTG TTCCAGACAC AGTAGGCTCC 
GATTCAGAGA GCTCTAGGCC AGGTACTCCA GAGAAATCTC AAGCTCCATC TAGAAGTGCT 
GGTCATTGGT TTGATTTTAA CGATTCCAAA GTGGAACCCA TCCGCGAGAC GGATATTGAA 
AAGCAATTCC AGGGCAAGGA ATCTGCATAT ATGCTTTTTT ATCGCAAGTG TGAAATGAAT 
AGACCACCAG AAGCTAAAGG CAATCCTCGC TTTGGAGTCC CAGAGCACAT TCTAAATGAC 
ATGGACCAAG AAAATATCGA GCTTCAGACG AAAAGAACTG AATCGGCCTT ACAGATCAAC 
TGCATGGACC TTTGTCTCCA TCTGTCTTCT CATTACCAAT ATGAAAATGG TGCCCTGCAT 
CCAGCACATC CCCGAAACAA CAGTAAGCTG GCAATTGCTA TAGACCGCAG GAGGACTGTA 
GAAGATCTAA AACAATTAGC TTTCCAGGTT CTTGATGAAT CTTTGGAAGG ACAAGTCCTG 
AGTATTGCTA GAGTTCTACC TGCTGGACTT CATATCTTTC AGAATCTGAT TGATGCCAAG 
ATGGATCTGG TCACTGCAGG AATTACAGAT GGAACTGACA TCTTTGTGTG GGATGGAAAG 
CAGGTTGGGG GTGTAATGAT TCAGAGTGGA GCAGAGTTCA CGCCAGTCCT GCTCACCATC 
CTGCGCCCAG TCCTGCAAAC AAATTCTTCA GCTGAGTTCA GTGAGAGTCA GAGAGCGTTC 
CCATCATGTG CCTGCTTGAA AGACATACAA AAAGAGCTTG CTGGCCCCAC CTGTGAGGAG 
TCAGTGTTGT GCTTCCCTTC GTCAGAGAAT AGTGAGCAGT GGAGTTTTTA TTCTGCTGAC 
GACTCAAACA AGAGCCTAAA AGAATTGGGG CTAAGAGATG GCAGCTCTAT TCTCTTGTTT 
GATACACGTT CGGATGGGGA TGTATATCTT GCATCAGAAA GACATTTATC TAAAGACGCG 
AAGCAGAACT TCCTCATGGT GCAGGACTGG TGCAGACAGC AGGATGAGAA AGAAACGGTT 
AAAATAATTA TCACTCCCAA CACTACTATA CAAGATATCC ATGTGAAAGC AGTCGAATTG 
CTGCAGCTAC AGGAAGAGTG TAGCAAAAAT GACACTTGCT TAAGACCCCT CCACAGAAAA 
GAGAAGCTTC TTCCCCCAGT GCCAGAAAGC CTAACTGTGC AGGAGGCAGA ACTGAAAAAT 
GGATGTACCT TAGGGCTCTG CAAAGGGAAA GCCCCCAGCC CCTCCCAGAT CTTCTTGTAT 
TTCATTTCCG GGACTGACCT GCAGGAGGGC AGCGAGCAGG AGATTATTTT AGAAGAAACT 
CTCACTGTGA GACAGAGCCT GGAAAGGATG CTGAATAAAG CAGGTTTGCC AGGTAAGGCA 
GGATGGCACC TGAGAAAGAT GGACTGGTGT TATGAAGCAG GAGAGGCTCT GAGCGATGAG 
GAGTCCACAC TGAAAGAGCT GAATATCTGC GCTGGAGACA CATTGGTGAT CACTGAAGGG 
AAACTTCCAC CCAAGGGTTT CCTGAAGCTT TCTATATGGC TGTACTGCCC CCATACACAG 
CAAGATGCTA TGAATCATGT GACCTGTGAA CCAGGTGCTC TACAGACCTC CACTACAGAA 
GATAATAACA TGCAAGAAGG TGGAACCTCT AATTTCTGCT CTGTTGGTGA GATAGAAATA 
TCAGGAGACT TATCATTACA GGATGTGAAA ATGCAGATCC TGACTTTGCC AGTTTTTGAA 
GCCCTAGGGA TCCCTTCTCC TGAGTTCCTC AGAGTCTGGA CCCTGGAAAA CAAGCATCTG 
GGGAAAATCC TGCGCTCGCC TCCAGTGTTA CTCAGTGAGT ACAAACTGGG GAGCAAGCCA 
GCACTATGCA TTGAGCCTTT GCAGGTGGAG GAATGTTTGG GTGCTAAAGA TCTTGTCTTG 
CGGGTGCAGC TGTCTGTCCC AGGGGAGCGT CGCTATTTTC CTCCCGTAGA TATTGTGTGG 
GATATATCCA GAGGCTGCAC AGCCCCAGCC TTGCTGCACA GAATTGCCAT GCATTGCTCC 
CTGCCAACAG ACAAGGTGGA AATTGCAAAG CACTTTCCAG AGAAATATGA GTGGCTTCCA 
ATATCCAGTT GGACCCAACA TGTCTCCAGA AAGAGGAAAA AGAAAAAGCT GGAAAGTCTA 
CAGGGGGCTC CTTATCACCT AAAGGATGGG GACATCATTG GGGTCAAGAA TCTTTTGCTT 
GATGACTGGA CAGACTTCAG CTCTGAAATT GACCGCATTG GAAAAGAAAA GCAGCAGCAG 
CAGCAGCGCC CAGCGAATGG AAACAAAAGT CTGCACGCAG AAGGATTGCA GAATAATACG 
GCTGGAAGCA ATGGAAGGGC AAAGTCGAAG GCCCGGAAAG CGGAGACCTC TCTCTCAATC 
CGAGTGGGCG TATTCAGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp40 ( XM_012971226.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826680.2 Xenopus tropicalis ubiquitin specific peptidase 40 (usp40), transcript variant X3, mRNA.

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CloneID	OXa66235
Clone ID Related Accession (Same CDS sequence)	XM_002932005.5 , XM_012971227.3 , XM_012971226.3
Accession Version	XM_012971226.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ubiquitin carboxyl-terminal hydrolase 40
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012971226.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGGAG ACCTATTTGA GGAAGATGAA GACTTTTCAT TCCTTTTGAG CAGTCCAAAC 
AAGAATGGCA AGAAATCCCA CCCCAGAGAT TCAAAGACCC CAGAGCCCCG AGGAGACACA 
AGGCTTAGTG GACTGAGGAA TCAAGGTGGC ACCTGTTACC TAAATTCTCT TCTGCAGACC 
CTGTTTTTCA CACCAGAATT CAGAGAAGCT CTTTTTGCTT TGGGTCCTAA AGAACTTGGA 
TCACTGGAGG AGAAAGACAC GCCAGACTCT CGGGTGCGAA TTATTCCCCT GCAGTTACAG 
CGTCTCTTCG CTCAACTTCT GCTGCTGGAT CAGCAGGCCT TGTCAACCAC AGATCTCACA 
GAGAGCTTTG GGTGGAACAG TAGTGAGGAG ACAAGCCAGC ATGATGTGCA AGAGTTAAAC 
CGTATTTTGT TCAGTGCTCT TGAGAGCTCA TTGGAGGGCA CATCTGGCCA TGACCTCATC 
AAGCGATTGT ACCATGGCAC CATTGTTAAC TGTATCCAGT GCAAGGAGTG TGGCTACATC 
AGTGAAAGGC AGGAGGATTT CCTTGACTTG ACGGTGGCAG TGAAGGGCGT GGGCAGTCTG 
GAGAATTCTC TGTGTAGCAT GTATTTGGAA GAGGAACACT TTGATGGAGA TAATTTGTAC 
AGATGTGGGA GCTGTGCCAA GCTTGTCCCT GCAACCAAGT CTGCCAAGTT GGGGAAACTG 
CCACCCTTTC TCACACTATC TCTGCTGAGG TTTAACTTTG ACTTTGTAAA GTGTGAGCGT 
TATAAGGAAA CTTCACGATA CACCTTTCCC ACCCGCCTCA TCATCCGGCC CTTTTGTGAG 
CAGCATAATC TAGAAGACTC CGTATATGCC TATGAGCTCT TCTCTGTTAT TATACACAAG 
GGCGGCTGTT ATGGAGGTCA TTATCATGCA TATATTCAAG ATGTCGATGA GCTGGGGCAT 
TGGGGACTCA AGGAAGTGGC TAAAAAGACA GCATCAGCAC AGGAGTCTGA GAGTAAACAA 
GATTGCCCAA TAGTTGCTCT TGCTTCCATC ATAGCAGAGG CTGGGGCCGA TCAAATGGTG 
GCAGTTGACC AGCTGGGACA GAAGCTATTG GAATACATGG GAACATCTTG GAATAAGACT 
TATCGCAAGC AGCATGGAGC TATGCACAAG TTCCTGCAAA GCCATCCCTC CGTTTTCCAG 
ATGAGTGCAG ATGGCTCGCT TGTAGGTTTG GCGCATAGTG TTCCAGACAC AGTAGGCTCC 
GATTCAGAGA GCTCTAGGCC AGGTACTCCA GAGAAATCTC AAGCTCCATC TAGAAGTGCT 
GGTCATTGGT TTGATTTTAA CGATTCCAAA GTGGAACCCA TCCGCGAGAC GGATATTGAA 
AAGCAATTCC AGGGCAAGGA ATCTGCATAT ATGCTTTTTT ATCGCAAGTG TGAAATGAAT 
AGACCACCAG AAGCTAAAGG CAATCCTCGC TTTGGAGTCC CAGAGCACAT TCTAAATGAC 
ATGGACCAAG AAAATATCGA GCTTCAGACG AAAAGAACTG AATCGGCCTT ACAGATCAAC 
TGCATGGACC TTTGTCTCCA TCTGTCTTCT CATTACCAAT ATGAAAATGG TGCCCTGCAT 
CCAGCACATC CCCGAAACAA CAGTAAGCTG GCAATTGCTA TAGACCGCAG GAGGACTGTA 
GAAGATCTAA AACAATTAGC TTTCCAGGTT CTTGATGAAT CTTTGGAAGG ACAAGTCCTG 
AGTATTGCTA GAGTTCTACC TGCTGGACTT CATATCTTTC AGAATCTGAT TGATGCCAAG 
ATGGATCTGG TCACTGCAGG AATTACAGAT GGAACTGACA TCTTTGTGTG GGATGGAAAG 
CAGGTTGGGG GTGTAATGAT TCAGAGTGGA GCAGAGTTCA CGCCAGTCCT GCTCACCATC 
CTGCGCCCAG TCCTGCAAAC AAATTCTTCA GCTGAGTTCA GTGAGAGTCA GAGAGCGTTC 
CCATCATGTG CCTGCTTGAA AGACATACAA AAAGAGCTTG CTGGCCCCAC CTGTGAGGAG 
TCAGTGTTGT GCTTCCCTTC GTCAGAGAAT AGTGAGCAGT GGAGTTTTTA TTCTGCTGAC 
GACTCAAACA AGAGCCTAAA AGAATTGGGG CTAAGAGATG GCAGCTCTAT TCTCTTGTTT 
GATACACGTT CGGATGGGGA TGTATATCTT GCATCAGAAA GACATTTATC TAAAGACGCG 
AAGCAGAACT TCCTCATGGT GCAGGACTGG TGCAGACAGC AGGATGAGAA AGAAACGGTT 
AAAATAATTA TCACTCCCAA CACTACTATA CAAGATATCC ATGTGAAAGC AGTCGAATTG 
CTGCAGCTAC AGGAAGAGTG TAGCAAAAAT GACACTTGCT TAAGACCCCT CCACAGAAAA 
GAGAAGCTTC TTCCCCCAGT GCCAGAAAGC CTAACTGTGC AGGAGGCAGA ACTGAAAAAT 
GGATGTACCT TAGGGCTCTG CAAAGGGAAA GCCCCCAGCC CCTCCCAGAT CTTCTTGTAT 
TTCATTTCCG GGACTGACCT GCAGGAGGGC AGCGAGCAGG AGATTATTTT AGAAGAAACT 
CTCACTGTGA GACAGAGCCT GGAAAGGATG CTGAATAAAG CAGGTTTGCC AGGTAAGGCA 
GGATGGCACC TGAGAAAGAT GGACTGGTGT TATGAAGCAG GAGAGGCTCT GAGCGATGAG 
GAGTCCACAC TGAAAGAGCT GAATATCTGC GCTGGAGACA CATTGGTGAT CACTGAAGGG 
AAACTTCCAC CCAAGGGTTT CCTGAAGCTT TCTATATGGC TGTACTGCCC CCATACACAG 
CAAGATGCTA TGAATCATGT GACCTGTGAA CCAGGTGCTC TACAGACCTC CACTACAGAA 
GATAATAACA TGCAAGAAGG TGGAACCTCT AATTTCTGCT CTGTTGGTGA GATAGAAATA 
TCAGGAGACT TATCATTACA GGATGTGAAA ATGCAGATCC TGACTTTGCC AGTTTTTGAA 
GCCCTAGGGA TCCCTTCTCC TGAGTTCCTC AGAGTCTGGA CCCTGGAAAA CAAGCATCTG 
GGGAAAATCC TGCGCTCGCC TCCAGTGTTA CTCAGTGAGT ACAAACTGGG GAGCAAGCCA 
GCACTATGCA TTGAGCCTTT GCAGGTGGAG GAATGTTTGG GTGCTAAAGA TCTTGTCTTG 
CGGGTGCAGC TGTCTGTCCC AGGGGAGCGT CGCTATTTTC CTCCCGTAGA TATTGTGTGG 
GATATATCCA GAGGCTGCAC AGCCCCAGCC TTGCTGCACA GAATTGCCAT GCATTGCTCC 
CTGCCAACAG ACAAGGTGGA AATTGCAAAG CACTTTCCAG AGAAATATGA GTGGCTTCCA 
ATATCCAGTT GGACCCAACA TGTCTCCAGA AAGAGGAAAA AGAAAAAGCT GGAAAGTCTA 
CAGGGGGCTC CTTATCACCT AAAGGATGGG GACATCATTG GGGTCAAGAA TCTTTTGCTT 
GATGACTGGA CAGACTTCAG CTCTGAAATT GACCGCATTG GAAAAGAAAA GCAGCAGCAG 
CAGCAGCGCC CAGCGAATGG AAACAAAAGT CTGCACGCAG AAGGATTGCA GAATAATACG 
GCTGGAAGCA ATGGAAGGGC AAAGTCGAAG GCCCGGAAAG CGGAGACCTC TCTCTCAATC 
CGAGTGGGCG TATTCAGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826680.2
CDS	956..4636
Translation	MFGDLFEEDEDFSFLLSSPNKNGKKSHPRDSKTPEPRGDTRLSGLRNQGGTCYLNSLLQTLFFTPEFREALFALGPKELGSLEEKDTPDSRVRIIPLQLQRLFAQLLLLDQQALSTTDLTESFGWNSSEETSQHDVQELNRILFSALESSLEGTSGHDLIKRLYHGTIVNCIQCKECGYISERQEDFLDLTVAVKGVGSLENSLCSMYLEEEHFDGDNLYRCGSCAKLVPATKSAKLGKLPPFLTLSLLRFNFDFVKCERYKETSRYTFPTRLIIRPFCEQHNLEDSVYAYELFSVIIHKGGCYGGHYHAYIQDVDELGHWGLKEVAKKTASAQESESKQDCPIVALASIIAEAGADQMVAVDQLGQKLLEYMGTSWNKTYRKQHGAMHKFLQSHPSVFQMSADGSLVGLAHSVPDTVGSDSESSRPGTPEKSQAPSRSAGHWFDFNDSKVEPIRETDIEKQFQGKESAYMLFYRKCEMNRPPEAKGNPRFGVPEHILNDMDQENIELQTKRTESALQINCMDLCLHLSSHYQYENGALHPAHPRNNSKLAIAIDRRRTVEDLKQLAFQVLDESLEGQVLSIARVLPAGLHIFQNLIDAKMDLVTAGITDGTDIFVWDGKQVGGVMIQSGAEFTPVLLTILRPVLQTNSSAEFSESQRAFPSCACLKDIQKELAGPTCEESVLCFPSSENSEQWSFYSADDSNKSLKELGLRDGSSILLFDTRSDGDVYLASERHLSKDAKQNFLMVQDWCRQQDEKETVKIIITPNTTIQDIHVKAVELLQLQEECSKNDTCLRPLHRKEKLLPPVPESLTVQEAELKNGCTLGLCKGKAPSPSQIFLYFISGTDLQEGSEQEIILEETLTVRQSLERMLNKAGLPGKAGWHLRKMDWCYEAGEALSDEESTLKELNICAGDTLVITEGKLPPKGFLKLSIWLYCPHTQQDAMNHVTCEPGALQTSTTEDNNMQEGGTSNFCSVGEIEISGDLSLQDVKMQILTLPVFEALGIPSPEFLRVWTLENKHLGKILRSPPVLLSEYKLGSKPALCIEPLQVEECLGAKDLVLRVQLSVPGERRYFPPVDIVWDISRGCTAPALLHRIAMHCSLPTDKVEIAKHFPEKYEWLPISSWTQHVSRKRKKKKLESLQGAPYHLKDGDIIGVKNLLLDDWTDFSSEIDRIGKEKQQQQQRPANGNKSLHAEGLQNNTAGSNGRAKSKARKAETSLSIRVGVFR

Target ORF information:

RefSeq Version	XM_012971226.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin specific peptidase 40 (usp40), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971226.3

ORF Insert Sequence:

ATGTTTGGAG ACCTATTTGA GGAAGATGAA GACTTTTCAT TCCTTTTGAG CAGTCCAAAC 
AAGAATGGCA AGAAATCCCA CCCCAGAGAT TCAAAGACCC CAGAGCCCCG AGGAGACACA 
AGGCTTAGTG GACTGAGGAA TCAAGGTGGC ACCTGTTACC TAAATTCTCT TCTGCAGACC 
CTGTTTTTCA CACCAGAATT CAGAGAAGCT CTTTTTGCTT TGGGTCCTAA AGAACTTGGA 
TCACTGGAGG AGAAAGACAC GCCAGACTCT CGGGTGCGAA TTATTCCCCT GCAGTTACAG 
CGTCTCTTCG CTCAACTTCT GCTGCTGGAT CAGCAGGCCT TGTCAACCAC AGATCTCACA 
GAGAGCTTTG GGTGGAACAG TAGTGAGGAG ACAAGCCAGC ATGATGTGCA AGAGTTAAAC 
CGTATTTTGT TCAGTGCTCT TGAGAGCTCA TTGGAGGGCA CATCTGGCCA TGACCTCATC 
AAGCGATTGT ACCATGGCAC CATTGTTAAC TGTATCCAGT GCAAGGAGTG TGGCTACATC 
AGTGAAAGGC AGGAGGATTT CCTTGACTTG ACGGTGGCAG TGAAGGGCGT GGGCAGTCTG 
GAGAATTCTC TGTGTAGCAT GTATTTGGAA GAGGAACACT TTGATGGAGA TAATTTGTAC 
AGATGTGGGA GCTGTGCCAA GCTTGTCCCT GCAACCAAGT CTGCCAAGTT GGGGAAACTG 
CCACCCTTTC TCACACTATC TCTGCTGAGG TTTAACTTTG ACTTTGTAAA GTGTGAGCGT 
TATAAGGAAA CTTCACGATA CACCTTTCCC ACCCGCCTCA TCATCCGGCC CTTTTGTGAG 
CAGCATAATC TAGAAGACTC CGTATATGCC TATGAGCTCT TCTCTGTTAT TATACACAAG 
GGCGGCTGTT ATGGAGGTCA TTATCATGCA TATATTCAAG ATGTCGATGA GCTGGGGCAT 
TGGGGACTCA AGGAAGTGGC TAAAAAGACA GCATCAGCAC AGGAGTCTGA GAGTAAACAA 
GATTGCCCAA TAGTTGCTCT TGCTTCCATC ATAGCAGAGG CTGGGGCCGA TCAAATGGTG 
GCAGTTGACC AGCTGGGACA GAAGCTATTG GAATACATGG GAACATCTTG GAATAAGACT 
TATCGCAAGC AGCATGGAGC TATGCACAAG TTCCTGCAAA GCCATCCCTC CGTTTTCCAG 
ATGAGTGCAG ATGGCTCGCT TGTAGGTTTG GCGCATAGTG TTCCAGACAC AGTAGGCTCC 
GATTCAGAGA GCTCTAGGCC AGGTACTCCA GAGAAATCTC AAGCTCCATC TAGAAGTGCT 
GGTCATTGGT TTGATTTTAA CGATTCCAAA GTGGAACCCA TCCGCGAGAC GGATATTGAA 
AAGCAATTCC AGGGCAAGGA ATCTGCATAT ATGCTTTTTT ATCGCAAGTG TGAAATGAAT 
AGACCACCAG AAGCTAAAGG CAATCCTCGC TTTGGAGTCC CAGAGCACAT TCTAAATGAC 
ATGGACCAAG AAAATATCGA GCTTCAGACG AAAAGAACTG AATCGGCCTT ACAGATCAAC 
TGCATGGACC TTTGTCTCCA TCTGTCTTCT CATTACCAAT ATGAAAATGG TGCCCTGCAT 
CCAGCACATC CCCGAAACAA CAGTAAGCTG GCAATTGCTA TAGACCGCAG GAGGACTGTA 
GAAGATCTAA AACAATTAGC TTTCCAGGTT CTTGATGAAT CTTTGGAAGG ACAAGTCCTG 
AGTATTGCTA GAGTTCTACC TGCTGGACTT CATATCTTTC AGAATCTGAT TGATGCCAAG 
ATGGATCTGG TCACTGCAGG AATTACAGAT GGAACTGACA TCTTTGTGTG GGATGGAAAG 
CAGGTTGGGG GTGTAATGAT TCAGAGTGGA GCAGAGTTCA CGCCAGTCCT GCTCACCATC 
CTGCGCCCAG TCCTGCAAAC AAATTCTTCA GCTGAGTTCA GTGAGAGTCA GAGAGCGTTC 
CCATCATGTG CCTGCTTGAA AGACATACAA AAAGAGCTTG CTGGCCCCAC CTGTGAGGAG 
TCAGTGTTGT GCTTCCCTTC GTCAGAGAAT AGTGAGCAGT GGAGTTTTTA TTCTGCTGAC 
GACTCAAACA AGAGCCTAAA AGAATTGGGG CTAAGAGATG GCAGCTCTAT TCTCTTGTTT 
GATACACGTT CGGATGGGGA TGTATATCTT GCATCAGAAA GACATTTATC TAAAGACGCG 
AAGCAGAACT TCCTCATGGT GCAGGACTGG TGCAGACAGC AGGATGAGAA AGAAACGGTT 
AAAATAATTA TCACTCCCAA CACTACTATA CAAGATATCC ATGTGAAAGC AGTCGAATTG 
CTGCAGCTAC AGGAAGAGTG TAGCAAAAAT GACACTTGCT TAAGACCCCT CCACAGAAAA 
GAGAAGCTTC TTCCCCCAGT GCCAGAAAGC CTAACTGTGC AGGAGGCAGA ACTGAAAAAT 
GGATGTACCT TAGGGCTCTG CAAAGGGAAA GCCCCCAGCC CCTCCCAGAT CTTCTTGTAT 
TTCATTTCCG GGACTGACCT GCAGGAGGGC AGCGAGCAGG AGATTATTTT AGAAGAAACT 
CTCACTGTGA GACAGAGCCT GGAAAGGATG CTGAATAAAG CAGGTTTGCC AGGTAAGGCA 
GGATGGCACC TGAGAAAGAT GGACTGGTGT TATGAAGCAG GAGAGGCTCT GAGCGATGAG 
GAGTCCACAC TGAAAGAGCT GAATATCTGC GCTGGAGACA CATTGGTGAT CACTGAAGGG 
AAACTTCCAC CCAAGGGTTT CCTGAAGCTT TCTATATGGC TGTACTGCCC CCATACACAG 
CAAGATGCTA TGAATCATGT GACCTGTGAA CCAGGTGCTC TACAGACCTC CACTACAGAA 
GATAATAACA TGCAAGAAGG TGGAACCTCT AATTTCTGCT CTGTTGGTGA GATAGAAATA 
TCAGGAGACT TATCATTACA GGATGTGAAA ATGCAGATCC TGACTTTGCC AGTTTTTGAA 
GCCCTAGGGA TCCCTTCTCC TGAGTTCCTC AGAGTCTGGA CCCTGGAAAA CAAGCATCTG 
GGGAAAATCC TGCGCTCGCC TCCAGTGTTA CTCAGTGAGT ACAAACTGGG GAGCAAGCCA 
GCACTATGCA TTGAGCCTTT GCAGGTGGAG GAATGTTTGG GTGCTAAAGA TCTTGTCTTG 
CGGGTGCAGC TGTCTGTCCC AGGGGAGCGT CGCTATTTTC CTCCCGTAGA TATTGTGTGG 
GATATATCCA GAGGCTGCAC AGCCCCAGCC TTGCTGCACA GAATTGCCAT GCATTGCTCC 
CTGCCAACAG ACAAGGTGGA AATTGCAAAG CACTTTCCAG AGAAATATGA GTGGCTTCCA 
ATATCCAGTT GGACCCAACA TGTCTCCAGA AAGAGGAAAA AGAAAAAGCT GGAAAGTCTA 
CAGGGGGCTC CTTATCACCT AAAGGATGGG GACATCATTG GGGTCAAGAA TCTTTTGCTT 
GATGACTGGA CAGACTTCAG CTCTGAAATT GACCGCATTG GAAAAGAAAA GCAGCAGCAG 
CAGCAGCGCC CAGCGAATGG AAACAAAAGT CTGCACGCAG AAGGATTGCA GAATAATACG 
GCTGGAAGCA ATGGAAGGGC AAAGTCGAAG GCCCGGAAAG CGGAGACCTC TCTCTCAATC 
CGAGTGGGCG TATTCAGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp40 ( XM_002932005.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002932051.2 Xenopus tropicalis ubiquitin specific peptidase 40 (usp40), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa16185
Clone ID Related Accession (Same CDS sequence)	XM_002932005.4 , XM_012971227.2 , XM_012971226.2
Accession Version	XM_002932005.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3684bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ubiquitin carboxyl-terminal hydrolase 40
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002932005.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGGAG ACCTATTTGA GGAAGATGAA GACTTTTCAT TCCTTTTGAG CAGTCCAAAC 
ACGAATGGCA AGAAATCCCA CCCCAGAGAT TCAAAGACCC CAGAGCCCCG AGGAGACACA 
AGGCTTAGTG GACTGAGGAA TCAAGGTGGC ACCTGTTACC TAAATTCTCT TCTGCAGACC 
CTGTTTTTCA CACCAGAATT CAGAGAAGCC CTTTTTGCTT TGGGTCCTAA AGAACTTGGA 
TCACTGGAGG AGAAAGACAC GCCAGACTCT CGGGTGCGAA TTATTCCCCT GCAGTTACAG 
CGTCTCTTCG CTCAACTTCT GCTGCTGGAT CAGCAGGCCT TGTCAACCAC AGATCTCACA 
GAGAGCTTTG GGTGGAACAG TAGTGAGGAG ACAAGCCAGC ATGATGTGCA AGAGTTAAAC 
CGTATTTTGT TCAGTGCTCT TGAGAGCTCA TTGGAGGGCA CATCTGGCCA TGACCTCATC 
AAGCGATTGT ACCATGGCAC CATTGTTAAC TGTATCCAGT GCAAGGAGTG TGGCTACATC 
AGTGAAAGGC AGGAGGATTT CCTTGACTTG ACGGTGGCAG TGAAGGGCGT GGGCAGTCTG 
GAGAATTCTC TGTGTAGCAT GTATTTGGAA GAGGAACACT TTGATGGAGA TAATTTGTAC 
AGATGTGGGA GCTGTGCCAA GCTTGTCCCT GCAACCAAGT CTGCCAAGTT GGGGAAACTG 
CCACCCTTTC TCACACTATC TCTGCTGAGG TTTAACTTTG ACTTTGTAAA GTGTGAGCGT 
TATAAGGAAA CTTCACGATA CACCTTTCCC ACCCGCCTCA TCATCCGGCC CTTTTGTGAG 
CAGCATAATC TAGAAGACTC CGTATATGCC TATGAGCTCT TCTCTGTTAT TATACACAAG 
GGCGGCTGTT ATGGAGGTCA TTATCATGCA TATATTCAAG ATGTCGATGA GCTGGGGCAT 
TGGGGACTCA AGGAAGTGGC TAAAAAGACA GCATCAGCAC AGGAGTCTGA GAGTAAACAA 
GATTGCCCAA TAGTTGCTCT TGCTTCCATC ATAGCAGAGG CTGGGGCCGA TCAAATGGTG 
GCAGTTGACC AGCTGGGACA GAAGCTATTG GAATACATGG GAACATCTTG GAATAAGACT 
TATCGCAAGC AGCATGGAGC TATGCACAAG TTCCTGCAAA GCCATCCCTC CGTTTTCCAG 
ATGAGTGCAG ATGGCTCGCT TGTAGGTTTG GCGCATAGTG TTCCAGACAC AGTAGGCTCC 
GATTCAGAGA GCTCTAGGCC AGGTACTCCA GAGAAATCTC AAGCTCCATC TAGAAGTGCT 
GGTCATTGGT TTGATTTTAA CGATTCCAAA GTGGAACCCA TCCGCGAGAC GGATATTGAA 
AAGCAATTCC AGGGCAAGGA ATCTGCATAT ATGCTTTTTT ATCGCAAGTG TGAAATGAAT 
AGACCACCAG AAGCTAAAGG CAATCCTCGC TTTGGAGTCC CAGAGCACAT TCTAAATGAC 
ATGGACCAAG AAAATATCGA GCTTCAGACG AAAAGAACTG AATCGGCCTT ACAGATCAAC 
TGCATGGACC TTTGTCTCCA TCTGTCTTCT CATTACCAAT ATGAAAATGG TGCCCTGCAT 
CCAGCACATC CCCGAAACAA CAGTAAGCTG GCAATTGCTA TAGACCGCAG GAGGACTGTA 
GAAGATCTAA AACAATTAGC TTTCCAGGTT CTTGATGAAT CTTTGGAAGG ACAAGTCCTG 
AGTATTGCTA GAGTTCTACC TGCTGGACTT CATATCTTTC AGAATCTGAT TGATGCCAAG 
ATGGATCTGG TCACTGCAGG AATTACAGAT GGAACTGACA TCTTTGTGTG GGATGGAAAG 
CAGGTTGGGG GTGTAATGAT TCAGAGTGGA GCAGAGTTCA CGCCAGTCCT GCTCACCATC 
CTGCGCCCAG TCCTGCAAAC AAATTCTTCA GCTGAGTTCA GTGAGAGTCA GAGAGCGTTC 
CCATCATGTG CCTGCTTGAA AGACATACAA AAAGAGCTTG CTGGCCCCAC CTGTGAGGAG 
TCAGTGTTGT GCTTCCCTTC GTCAGAGAAT AGTGAGCAGT GGAGTTTTTA TTCTGCTGAC 
GACTCAAACA AGAGCCTAAA AGAATTGGGG CTAAGAGATG GCAGCTCTAT TCTCTTGTTT 
GATACACGTT CGGATGGGGA TGTATATCTT GCATCAGAAA GACATTTATC TAAAGACGCG 
AAGCAGAACT TCCTCATGGT GCAGGACTGG TGCAGACAGC AGGATGAGAA AGAAACGGTT 
AAAATAATTA TCACTCCCAA CACTACTATA CAAGATATCC ATGTGAAAGC AGTCGAATTG 
CTGCAGCTAC AGGAAGAGTG TAGCAAAAAT GACACTTGCT TAAGACCCCT CCACAGAAAA 
GAGAAGCTTC TTCCCCCAGT GCCAGAAAGC CTAACTGTGC AGGAGGCAGA ACTGAAAAAT 
GGATGTACCT TAGGGCTCTG CAAAGGGAAA GCCCCCAGCC CCTCCCAGAT CTTCTTGTAT 
TTCATTTCCG GGACTGACCT GCAGGAGGGC AGCGAGCAGG AGATTATTTT AGAAGAAACT 
CTCACTGTGA GACAGAGCCT GGAAAGGATG CTGAATAAAG CAGGTTTGCC AGGTAAGGCA 
GGATGGCACC TGAGAAAGAT GGACTGGTGT TATGAAGCAG GAGAGGCTCT GAGCGATGAG 
GAGTCCACAC TGAAAGAGCT GAATATCTGC GCTGGAGACA CATTGGTGAT CACTGAAGGG 
AAACTTCCAC CCAAGGGTTT CCTGAAGCTT TCTATATGGC TGTACTGCCC CCATACACAG 
CAAGATGCTA TGAATCATGT GACCTGTGAA CCAGGTGCTC TACAGACCTC CACTACAGAA 
GATAATAACA TGCAAGAAGG TGGAACCTCT AATTTCTGCT CTGTTGGTGA GATAGAAATA 
TCAGGAGACT TATCATTACA GGATGTGAAA ATGCAGATCC TGACTTTGCC AGTTTTTGAA 
GCCCTAGGGA TCCCTTCTCC TGAGTTCCTC AGAGTCTGGA CCCTGGAAAA CAAGCATCTG 
GGGAAAATCC TGCGCTCGCC TCCAGTGTTA CTCAGTGAGT ACAAACTGGG GAGCAAGCCA 
GCACTATGCA TTGAGCCTTT GCAGGTGGAG GAATGTTTGG GTGCTAAAGA TCTTGTCTTG 
CGGGTGCAGC TGTCTGTCCC AGGGGAGTGT CGCTATTTTC CTCCCGTAGA TATTGTGTGG 
GATATATCTA GAGGCTGCAC AGCCCCAGCC TTGCTGCACA GAATTGCCAT GCATTGCTCC 
CTGCCAACAG ACAAGGTGGA AATTGCAAAG CACTTTCCAG AGAAATATGA GTGGCTTCCA 
ATATCCAGTT GGACCCAACA TGTCTCCAGA AAGAGGAAAA AGAAAAAGCT GGAAAGTCTA 
CAGGGGGCTC CTTATCACCT AAAGGATGGG GACATCATTG GGGTCAAGAA TCTTTTGCTT 
GATGACTGGA CAGACTTCAG CTCTGAAATT GACCGCATTG GAAAAGAAAA GCAGCAGCAG 
CAGCAGCAGC GCCCAGCGAA TGGAAACAAA AGTCTGCACG CAGAAGGATT GCAGAATAAT 
ACGGCTGGAA GCAATGGAAG GGCAAAGTCG AAGGCCCGGA AAGCGGAGAC CTCTCTCTCA 
ATCCGAGTGG GCGTATTCAG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002932051.2
CDS	1587..5270
Translation	MFGDLFEEDEDFSFLLSSPNTNGKKSHPRDSKTPEPRGDTRLSGLRNQGGTCYLNSLLQTLFFTPEFREALFALGPKELGSLEEKDTPDSRVRIIPLQLQRLFAQLLLLDQQALSTTDLTESFGWNSSEETSQHDVQELNRILFSALESSLEGTSGHDLIKRLYHGTIVNCIQCKECGYISERQEDFLDLTVAVKGVGSLENSLCSMYLEEEHFDGDNLYRCGSCAKLVPATKSAKLGKLPPFLTLSLLRFNFDFVKCERYKETSRYTFPTRLIIRPFCEQHNLEDSVYAYELFSVIIHKGGCYGGHYHAYIQDVDELGHWGLKEVAKKTASAQESESKQDCPIVALASIIAEAGADQMVAVDQLGQKLLEYMGTSWNKTYRKQHGAMHKFLQSHPSVFQMSADGSLVGLAHSVPDTVGSDSESSRPGTPEKSQAPSRSAGHWFDFNDSKVEPIRETDIEKQFQGKESAYMLFYRKCEMNRPPEAKGNPRFGVPEHILNDMDQENIELQTKRTESALQINCMDLCLHLSSHYQYENGALHPAHPRNNSKLAIAIDRRRTVEDLKQLAFQVLDESLEGQVLSIARVLPAGLHIFQNLIDAKMDLVTAGITDGTDIFVWDGKQVGGVMIQSGAEFTPVLLTILRPVLQTNSSAEFSESQRAFPSCACLKDIQKELAGPTCEESVLCFPSSENSEQWSFYSADDSNKSLKELGLRDGSSILLFDTRSDGDVYLASERHLSKDAKQNFLMVQDWCRQQDEKETVKIIITPNTTIQDIHVKAVELLQLQEECSKNDTCLRPLHRKEKLLPPVPESLTVQEAELKNGCTLGLCKGKAPSPSQIFLYFISGTDLQEGSEQEIILEETLTVRQSLERMLNKAGLPGKAGWHLRKMDWCYEAGEALSDEESTLKELNICAGDTLVITEGKLPPKGFLKLSIWLYCPHTQQDAMNHVTCEPGALQTSTTEDNNMQEGGTSNFCSVGEIEISGDLSLQDVKMQILTLPVFEALGIPSPEFLRVWTLENKHLGKILRSPPVLLSEYKLGSKPALCIEPLQVEECLGAKDLVLRVQLSVPGECRYFPPVDIVWDISRGCTAPALLHRIAMHCSLPTDKVEIAKHFPEKYEWLPISSWTQHVSRKRKKKKLESLQGAPYHLKDGDIIGVKNLLLDDWTDFSSEIDRIGKEKQQQQQQRPANGNKSLHAEGLQNNTAGSNGRAKSKARKAETSLSIRVGVFR

Target ORF information:

RefSeq Version	XM_002932005.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin specific peptidase 40 (usp40), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002932005.4

ORF Insert Sequence:

ATGTTTGGAG ACCTATTTGA GGAAGATGAA GACTTTTCAT TCCTTTTGAG CAGTCCAAAC 
ACGAATGGCA AGAAATCCCA CCCCAGAGAT TCAAAGACCC CAGAGCCCCG AGGAGACACA 
AGGCTTAGTG GACTGAGGAA TCAAGGTGGC ACCTGTTACC TAAATTCTCT TCTGCAGACC 
CTGTTTTTCA CACCAGAATT CAGAGAAGCC CTTTTTGCTT TGGGTCCTAA AGAACTTGGA 
TCACTGGAGG AGAAAGACAC GCCAGACTCT CGGGTGCGAA TTATTCCCCT GCAGTTACAG 
CGTCTCTTCG CTCAACTTCT GCTGCTGGAT CAGCAGGCCT TGTCAACCAC AGATCTCACA 
GAGAGCTTTG GGTGGAACAG TAGTGAGGAG ACAAGCCAGC ATGATGTGCA AGAGTTAAAC 
CGTATTTTGT TCAGTGCTCT TGAGAGCTCA TTGGAGGGCA CATCTGGCCA TGACCTCATC 
AAGCGATTGT ACCATGGCAC CATTGTTAAC TGTATCCAGT GCAAGGAGTG TGGCTACATC 
AGTGAAAGGC AGGAGGATTT CCTTGACTTG ACGGTGGCAG TGAAGGGCGT GGGCAGTCTG 
GAGAATTCTC TGTGTAGCAT GTATTTGGAA GAGGAACACT TTGATGGAGA TAATTTGTAC 
AGATGTGGGA GCTGTGCCAA GCTTGTCCCT GCAACCAAGT CTGCCAAGTT GGGGAAACTG 
CCACCCTTTC TCACACTATC TCTGCTGAGG TTTAACTTTG ACTTTGTAAA GTGTGAGCGT 
TATAAGGAAA CTTCACGATA CACCTTTCCC ACCCGCCTCA TCATCCGGCC CTTTTGTGAG 
CAGCATAATC TAGAAGACTC CGTATATGCC TATGAGCTCT TCTCTGTTAT TATACACAAG 
GGCGGCTGTT ATGGAGGTCA TTATCATGCA TATATTCAAG ATGTCGATGA GCTGGGGCAT 
TGGGGACTCA AGGAAGTGGC TAAAAAGACA GCATCAGCAC AGGAGTCTGA GAGTAAACAA 
GATTGCCCAA TAGTTGCTCT TGCTTCCATC ATAGCAGAGG CTGGGGCCGA TCAAATGGTG 
GCAGTTGACC AGCTGGGACA GAAGCTATTG GAATACATGG GAACATCTTG GAATAAGACT 
TATCGCAAGC AGCATGGAGC TATGCACAAG TTCCTGCAAA GCCATCCCTC CGTTTTCCAG 
ATGAGTGCAG ATGGCTCGCT TGTAGGTTTG GCGCATAGTG TTCCAGACAC AGTAGGCTCC 
GATTCAGAGA GCTCTAGGCC AGGTACTCCA GAGAAATCTC AAGCTCCATC TAGAAGTGCT 
GGTCATTGGT TTGATTTTAA CGATTCCAAA GTGGAACCCA TCCGCGAGAC GGATATTGAA 
AAGCAATTCC AGGGCAAGGA ATCTGCATAT ATGCTTTTTT ATCGCAAGTG TGAAATGAAT 
AGACCACCAG AAGCTAAAGG CAATCCTCGC TTTGGAGTCC CAGAGCACAT TCTAAATGAC 
ATGGACCAAG AAAATATCGA GCTTCAGACG AAAAGAACTG AATCGGCCTT ACAGATCAAC 
TGCATGGACC TTTGTCTCCA TCTGTCTTCT CATTACCAAT ATGAAAATGG TGCCCTGCAT 
CCAGCACATC CCCGAAACAA CAGTAAGCTG GCAATTGCTA TAGACCGCAG GAGGACTGTA 
GAAGATCTAA AACAATTAGC TTTCCAGGTT CTTGATGAAT CTTTGGAAGG ACAAGTCCTG 
AGTATTGCTA GAGTTCTACC TGCTGGACTT CATATCTTTC AGAATCTGAT TGATGCCAAG 
ATGGATCTGG TCACTGCAGG AATTACAGAT GGAACTGACA TCTTTGTGTG GGATGGAAAG 
CAGGTTGGGG GTGTAATGAT TCAGAGTGGA GCAGAGTTCA CGCCAGTCCT GCTCACCATC 
CTGCGCCCAG TCCTGCAAAC AAATTCTTCA GCTGAGTTCA GTGAGAGTCA GAGAGCGTTC 
CCATCATGTG CCTGCTTGAA AGACATACAA AAAGAGCTTG CTGGCCCCAC CTGTGAGGAG 
TCAGTGTTGT GCTTCCCTTC GTCAGAGAAT AGTGAGCAGT GGAGTTTTTA TTCTGCTGAC 
GACTCAAACA AGAGCCTAAA AGAATTGGGG CTAAGAGATG GCAGCTCTAT TCTCTTGTTT 
GATACACGTT CGGATGGGGA TGTATATCTT GCATCAGAAA GACATTTATC TAAAGACGCG 
AAGCAGAACT TCCTCATGGT GCAGGACTGG TGCAGACAGC AGGATGAGAA AGAAACGGTT 
AAAATAATTA TCACTCCCAA CACTACTATA CAAGATATCC ATGTGAAAGC AGTCGAATTG 
CTGCAGCTAC AGGAAGAGTG TAGCAAAAAT GACACTTGCT TAAGACCCCT CCACAGAAAA 
GAGAAGCTTC TTCCCCCAGT GCCAGAAAGC CTAACTGTGC AGGAGGCAGA ACTGAAAAAT 
GGATGTACCT TAGGGCTCTG CAAAGGGAAA GCCCCCAGCC CCTCCCAGAT CTTCTTGTAT 
TTCATTTCCG GGACTGACCT GCAGGAGGGC AGCGAGCAGG AGATTATTTT AGAAGAAACT 
CTCACTGTGA GACAGAGCCT GGAAAGGATG CTGAATAAAG CAGGTTTGCC AGGTAAGGCA 
GGATGGCACC TGAGAAAGAT GGACTGGTGT TATGAAGCAG GAGAGGCTCT GAGCGATGAG 
GAGTCCACAC TGAAAGAGCT GAATATCTGC GCTGGAGACA CATTGGTGAT CACTGAAGGG 
AAACTTCCAC CCAAGGGTTT CCTGAAGCTT TCTATATGGC TGTACTGCCC CCATACACAG 
CAAGATGCTA TGAATCATGT GACCTGTGAA CCAGGTGCTC TACAGACCTC CACTACAGAA 
GATAATAACA TGCAAGAAGG TGGAACCTCT AATTTCTGCT CTGTTGGTGA GATAGAAATA 
TCAGGAGACT TATCATTACA GGATGTGAAA ATGCAGATCC TGACTTTGCC AGTTTTTGAA 
GCCCTAGGGA TCCCTTCTCC TGAGTTCCTC AGAGTCTGGA CCCTGGAAAA CAAGCATCTG 
GGGAAAATCC TGCGCTCGCC TCCAGTGTTA CTCAGTGAGT ACAAACTGGG GAGCAAGCCA 
GCACTATGCA TTGAGCCTTT GCAGGTGGAG GAATGTTTGG GTGCTAAAGA TCTTGTCTTG 
CGGGTGCAGC TGTCTGTCCC AGGGGAGTGT CGCTATTTTC CTCCCGTAGA TATTGTGTGG 
GATATATCTA GAGGCTGCAC AGCCCCAGCC TTGCTGCACA GAATTGCCAT GCATTGCTCC 
CTGCCAACAG ACAAGGTGGA AATTGCAAAG CACTTTCCAG AGAAATATGA GTGGCTTCCA 
ATATCCAGTT GGACCCAACA TGTCTCCAGA AAGAGGAAAA AGAAAAAGCT GGAAAGTCTA 
CAGGGGGCTC CTTATCACCT AAAGGATGGG GACATCATTG GGGTCAAGAA TCTTTTGCTT 
GATGACTGGA CAGACTTCAG CTCTGAAATT GACCGCATTG GAAAAGAAAA GCAGCAGCAG 
CAGCAGCAGC GCCCAGCGAA TGGAAACAAA AGTCTGCACG CAGAAGGATT GCAGAATAAT 
ACGGCTGGAA GCAATGGAAG GGCAAAGTCG AAGGCCCGGA AAGCGGAGAC CTCTCTCTCA 
ATCCGAGTGG GCGTATTCAG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp40 ( XM_012971227.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826681.1 Xenopus tropicalis ubiquitin specific peptidase 40 (usp40), transcript variant X1, mRNA.

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CloneID	OXa16185
Clone ID Related Accession (Same CDS sequence)	XM_002932005.4 , XM_012971227.2 , XM_012971226.2
Accession Version	XM_012971227.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3684bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ubiquitin carboxyl-terminal hydrolase 40
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012971227.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGGAG ACCTATTTGA GGAAGATGAA GACTTTTCAT TCCTTTTGAG CAGTCCAAAC 
ACGAATGGCA AGAAATCCCA CCCCAGAGAT TCAAAGACCC CAGAGCCCCG AGGAGACACA 
AGGCTTAGTG GACTGAGGAA TCAAGGTGGC ACCTGTTACC TAAATTCTCT TCTGCAGACC 
CTGTTTTTCA CACCAGAATT CAGAGAAGCC CTTTTTGCTT TGGGTCCTAA AGAACTTGGA 
TCACTGGAGG AGAAAGACAC GCCAGACTCT CGGGTGCGAA TTATTCCCCT GCAGTTACAG 
CGTCTCTTCG CTCAACTTCT GCTGCTGGAT CAGCAGGCCT TGTCAACCAC AGATCTCACA 
GAGAGCTTTG GGTGGAACAG TAGTGAGGAG ACAAGCCAGC ATGATGTGCA AGAGTTAAAC 
CGTATTTTGT TCAGTGCTCT TGAGAGCTCA TTGGAGGGCA CATCTGGCCA TGACCTCATC 
AAGCGATTGT ACCATGGCAC CATTGTTAAC TGTATCCAGT GCAAGGAGTG TGGCTACATC 
AGTGAAAGGC AGGAGGATTT CCTTGACTTG ACGGTGGCAG TGAAGGGCGT GGGCAGTCTG 
GAGAATTCTC TGTGTAGCAT GTATTTGGAA GAGGAACACT TTGATGGAGA TAATTTGTAC 
AGATGTGGGA GCTGTGCCAA GCTTGTCCCT GCAACCAAGT CTGCCAAGTT GGGGAAACTG 
CCACCCTTTC TCACACTATC TCTGCTGAGG TTTAACTTTG ACTTTGTAAA GTGTGAGCGT 
TATAAGGAAA CTTCACGATA CACCTTTCCC ACCCGCCTCA TCATCCGGCC CTTTTGTGAG 
CAGCATAATC TAGAAGACTC CGTATATGCC TATGAGCTCT TCTCTGTTAT TATACACAAG 
GGCGGCTGTT ATGGAGGTCA TTATCATGCA TATATTCAAG ATGTCGATGA GCTGGGGCAT 
TGGGGACTCA AGGAAGTGGC TAAAAAGACA GCATCAGCAC AGGAGTCTGA GAGTAAACAA 
GATTGCCCAA TAGTTGCTCT TGCTTCCATC ATAGCAGAGG CTGGGGCCGA TCAAATGGTG 
GCAGTTGACC AGCTGGGACA GAAGCTATTG GAATACATGG GAACATCTTG GAATAAGACT 
TATCGCAAGC AGCATGGAGC TATGCACAAG TTCCTGCAAA GCCATCCCTC CGTTTTCCAG 
ATGAGTGCAG ATGGCTCGCT TGTAGGTTTG GCGCATAGTG TTCCAGACAC AGTAGGCTCC 
GATTCAGAGA GCTCTAGGCC AGGTACTCCA GAGAAATCTC AAGCTCCATC TAGAAGTGCT 
GGTCATTGGT TTGATTTTAA CGATTCCAAA GTGGAACCCA TCCGCGAGAC GGATATTGAA 
AAGCAATTCC AGGGCAAGGA ATCTGCATAT ATGCTTTTTT ATCGCAAGTG TGAAATGAAT 
AGACCACCAG AAGCTAAAGG CAATCCTCGC TTTGGAGTCC CAGAGCACAT TCTAAATGAC 
ATGGACCAAG AAAATATCGA GCTTCAGACG AAAAGAACTG AATCGGCCTT ACAGATCAAC 
TGCATGGACC TTTGTCTCCA TCTGTCTTCT CATTACCAAT ATGAAAATGG TGCCCTGCAT 
CCAGCACATC CCCGAAACAA CAGTAAGCTG GCAATTGCTA TAGACCGCAG GAGGACTGTA 
GAAGATCTAA AACAATTAGC TTTCCAGGTT CTTGATGAAT CTTTGGAAGG ACAAGTCCTG 
AGTATTGCTA GAGTTCTACC TGCTGGACTT CATATCTTTC AGAATCTGAT TGATGCCAAG 
ATGGATCTGG TCACTGCAGG AATTACAGAT GGAACTGACA TCTTTGTGTG GGATGGAAAG 
CAGGTTGGGG GTGTAATGAT TCAGAGTGGA GCAGAGTTCA CGCCAGTCCT GCTCACCATC 
CTGCGCCCAG TCCTGCAAAC AAATTCTTCA GCTGAGTTCA GTGAGAGTCA GAGAGCGTTC 
CCATCATGTG CCTGCTTGAA AGACATACAA AAAGAGCTTG CTGGCCCCAC CTGTGAGGAG 
TCAGTGTTGT GCTTCCCTTC GTCAGAGAAT AGTGAGCAGT GGAGTTTTTA TTCTGCTGAC 
GACTCAAACA AGAGCCTAAA AGAATTGGGG CTAAGAGATG GCAGCTCTAT TCTCTTGTTT 
GATACACGTT CGGATGGGGA TGTATATCTT GCATCAGAAA GACATTTATC TAAAGACGCG 
AAGCAGAACT TCCTCATGGT GCAGGACTGG TGCAGACAGC AGGATGAGAA AGAAACGGTT 
AAAATAATTA TCACTCCCAA CACTACTATA CAAGATATCC ATGTGAAAGC AGTCGAATTG 
CTGCAGCTAC AGGAAGAGTG TAGCAAAAAT GACACTTGCT TAAGACCCCT CCACAGAAAA 
GAGAAGCTTC TTCCCCCAGT GCCAGAAAGC CTAACTGTGC AGGAGGCAGA ACTGAAAAAT 
GGATGTACCT TAGGGCTCTG CAAAGGGAAA GCCCCCAGCC CCTCCCAGAT CTTCTTGTAT 
TTCATTTCCG GGACTGACCT GCAGGAGGGC AGCGAGCAGG AGATTATTTT AGAAGAAACT 
CTCACTGTGA GACAGAGCCT GGAAAGGATG CTGAATAAAG CAGGTTTGCC AGGTAAGGCA 
GGATGGCACC TGAGAAAGAT GGACTGGTGT TATGAAGCAG GAGAGGCTCT GAGCGATGAG 
GAGTCCACAC TGAAAGAGCT GAATATCTGC GCTGGAGACA CATTGGTGAT CACTGAAGGG 
AAACTTCCAC CCAAGGGTTT CCTGAAGCTT TCTATATGGC TGTACTGCCC CCATACACAG 
CAAGATGCTA TGAATCATGT GACCTGTGAA CCAGGTGCTC TACAGACCTC CACTACAGAA 
GATAATAACA TGCAAGAAGG TGGAACCTCT AATTTCTGCT CTGTTGGTGA GATAGAAATA 
TCAGGAGACT TATCATTACA GGATGTGAAA ATGCAGATCC TGACTTTGCC AGTTTTTGAA 
GCCCTAGGGA TCCCTTCTCC TGAGTTCCTC AGAGTCTGGA CCCTGGAAAA CAAGCATCTG 
GGGAAAATCC TGCGCTCGCC TCCAGTGTTA CTCAGTGAGT ACAAACTGGG GAGCAAGCCA 
GCACTATGCA TTGAGCCTTT GCAGGTGGAG GAATGTTTGG GTGCTAAAGA TCTTGTCTTG 
CGGGTGCAGC TGTCTGTCCC AGGGGAGTGT CGCTATTTTC CTCCCGTAGA TATTGTGTGG 
GATATATCTA GAGGCTGCAC AGCCCCAGCC TTGCTGCACA GAATTGCCAT GCATTGCTCC 
CTGCCAACAG ACAAGGTGGA AATTGCAAAG CACTTTCCAG AGAAATATGA GTGGCTTCCA 
ATATCCAGTT GGACCCAACA TGTCTCCAGA AAGAGGAAAA AGAAAAAGCT GGAAAGTCTA 
CAGGGGGCTC CTTATCACCT AAAGGATGGG GACATCATTG GGGTCAAGAA TCTTTTGCTT 
GATGACTGGA CAGACTTCAG CTCTGAAATT GACCGCATTG GAAAAGAAAA GCAGCAGCAG 
CAGCAGCAGC GCCCAGCGAA TGGAAACAAA AGTCTGCACG CAGAAGGATT GCAGAATAAT 
ACGGCTGGAA GCAATGGAAG GGCAAAGTCG AAGGCCCGGA AAGCGGAGAC CTCTCTCTCA 
ATCCGAGTGG GCGTATTCAG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826681.1
CDS	901..4584
Translation	MFGDLFEEDEDFSFLLSSPNTNGKKSHPRDSKTPEPRGDTRLSGLRNQGGTCYLNSLLQTLFFTPEFREALFALGPKELGSLEEKDTPDSRVRIIPLQLQRLFAQLLLLDQQALSTTDLTESFGWNSSEETSQHDVQELNRILFSALESSLEGTSGHDLIKRLYHGTIVNCIQCKECGYISERQEDFLDLTVAVKGVGSLENSLCSMYLEEEHFDGDNLYRCGSCAKLVPATKSAKLGKLPPFLTLSLLRFNFDFVKCERYKETSRYTFPTRLIIRPFCEQHNLEDSVYAYELFSVIIHKGGCYGGHYHAYIQDVDELGHWGLKEVAKKTASAQESESKQDCPIVALASIIAEAGADQMVAVDQLGQKLLEYMGTSWNKTYRKQHGAMHKFLQSHPSVFQMSADGSLVGLAHSVPDTVGSDSESSRPGTPEKSQAPSRSAGHWFDFNDSKVEPIRETDIEKQFQGKESAYMLFYRKCEMNRPPEAKGNPRFGVPEHILNDMDQENIELQTKRTESALQINCMDLCLHLSSHYQYENGALHPAHPRNNSKLAIAIDRRRTVEDLKQLAFQVLDESLEGQVLSIARVLPAGLHIFQNLIDAKMDLVTAGITDGTDIFVWDGKQVGGVMIQSGAEFTPVLLTILRPVLQTNSSAEFSESQRAFPSCACLKDIQKELAGPTCEESVLCFPSSENSEQWSFYSADDSNKSLKELGLRDGSSILLFDTRSDGDVYLASERHLSKDAKQNFLMVQDWCRQQDEKETVKIIITPNTTIQDIHVKAVELLQLQEECSKNDTCLRPLHRKEKLLPPVPESLTVQEAELKNGCTLGLCKGKAPSPSQIFLYFISGTDLQEGSEQEIILEETLTVRQSLERMLNKAGLPGKAGWHLRKMDWCYEAGEALSDEESTLKELNICAGDTLVITEGKLPPKGFLKLSIWLYCPHTQQDAMNHVTCEPGALQTSTTEDNNMQEGGTSNFCSVGEIEISGDLSLQDVKMQILTLPVFEALGIPSPEFLRVWTLENKHLGKILRSPPVLLSEYKLGSKPALCIEPLQVEECLGAKDLVLRVQLSVPGECRYFPPVDIVWDISRGCTAPALLHRIAMHCSLPTDKVEIAKHFPEKYEWLPISSWTQHVSRKRKKKKLESLQGAPYHLKDGDIIGVKNLLLDDWTDFSSEIDRIGKEKQQQQQQRPANGNKSLHAEGLQNNTAGSNGRAKSKARKAETSLSIRVGVFR

Target ORF information:

RefSeq Version	XM_012971227.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin specific peptidase 40 (usp40), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971227.2

ORF Insert Sequence:

ATGTTTGGAG ACCTATTTGA GGAAGATGAA GACTTTTCAT TCCTTTTGAG CAGTCCAAAC 
ACGAATGGCA AGAAATCCCA CCCCAGAGAT TCAAAGACCC CAGAGCCCCG AGGAGACACA 
AGGCTTAGTG GACTGAGGAA TCAAGGTGGC ACCTGTTACC TAAATTCTCT TCTGCAGACC 
CTGTTTTTCA CACCAGAATT CAGAGAAGCC CTTTTTGCTT TGGGTCCTAA AGAACTTGGA 
TCACTGGAGG AGAAAGACAC GCCAGACTCT CGGGTGCGAA TTATTCCCCT GCAGTTACAG 
CGTCTCTTCG CTCAACTTCT GCTGCTGGAT CAGCAGGCCT TGTCAACCAC AGATCTCACA 
GAGAGCTTTG GGTGGAACAG TAGTGAGGAG ACAAGCCAGC ATGATGTGCA AGAGTTAAAC 
CGTATTTTGT TCAGTGCTCT TGAGAGCTCA TTGGAGGGCA CATCTGGCCA TGACCTCATC 
AAGCGATTGT ACCATGGCAC CATTGTTAAC TGTATCCAGT GCAAGGAGTG TGGCTACATC 
AGTGAAAGGC AGGAGGATTT CCTTGACTTG ACGGTGGCAG TGAAGGGCGT GGGCAGTCTG 
GAGAATTCTC TGTGTAGCAT GTATTTGGAA GAGGAACACT TTGATGGAGA TAATTTGTAC 
AGATGTGGGA GCTGTGCCAA GCTTGTCCCT GCAACCAAGT CTGCCAAGTT GGGGAAACTG 
CCACCCTTTC TCACACTATC TCTGCTGAGG TTTAACTTTG ACTTTGTAAA GTGTGAGCGT 
TATAAGGAAA CTTCACGATA CACCTTTCCC ACCCGCCTCA TCATCCGGCC CTTTTGTGAG 
CAGCATAATC TAGAAGACTC CGTATATGCC TATGAGCTCT TCTCTGTTAT TATACACAAG 
GGCGGCTGTT ATGGAGGTCA TTATCATGCA TATATTCAAG ATGTCGATGA GCTGGGGCAT 
TGGGGACTCA AGGAAGTGGC TAAAAAGACA GCATCAGCAC AGGAGTCTGA GAGTAAACAA 
GATTGCCCAA TAGTTGCTCT TGCTTCCATC ATAGCAGAGG CTGGGGCCGA TCAAATGGTG 
GCAGTTGACC AGCTGGGACA GAAGCTATTG GAATACATGG GAACATCTTG GAATAAGACT 
TATCGCAAGC AGCATGGAGC TATGCACAAG TTCCTGCAAA GCCATCCCTC CGTTTTCCAG 
ATGAGTGCAG ATGGCTCGCT TGTAGGTTTG GCGCATAGTG TTCCAGACAC AGTAGGCTCC 
GATTCAGAGA GCTCTAGGCC AGGTACTCCA GAGAAATCTC AAGCTCCATC TAGAAGTGCT 
GGTCATTGGT TTGATTTTAA CGATTCCAAA GTGGAACCCA TCCGCGAGAC GGATATTGAA 
AAGCAATTCC AGGGCAAGGA ATCTGCATAT ATGCTTTTTT ATCGCAAGTG TGAAATGAAT 
AGACCACCAG AAGCTAAAGG CAATCCTCGC TTTGGAGTCC CAGAGCACAT TCTAAATGAC 
ATGGACCAAG AAAATATCGA GCTTCAGACG AAAAGAACTG AATCGGCCTT ACAGATCAAC 
TGCATGGACC TTTGTCTCCA TCTGTCTTCT CATTACCAAT ATGAAAATGG TGCCCTGCAT 
CCAGCACATC CCCGAAACAA CAGTAAGCTG GCAATTGCTA TAGACCGCAG GAGGACTGTA 
GAAGATCTAA AACAATTAGC TTTCCAGGTT CTTGATGAAT CTTTGGAAGG ACAAGTCCTG 
AGTATTGCTA GAGTTCTACC TGCTGGACTT CATATCTTTC AGAATCTGAT TGATGCCAAG 
ATGGATCTGG TCACTGCAGG AATTACAGAT GGAACTGACA TCTTTGTGTG GGATGGAAAG 
CAGGTTGGGG GTGTAATGAT TCAGAGTGGA GCAGAGTTCA CGCCAGTCCT GCTCACCATC 
CTGCGCCCAG TCCTGCAAAC AAATTCTTCA GCTGAGTTCA GTGAGAGTCA GAGAGCGTTC 
CCATCATGTG CCTGCTTGAA AGACATACAA AAAGAGCTTG CTGGCCCCAC CTGTGAGGAG 
TCAGTGTTGT GCTTCCCTTC GTCAGAGAAT AGTGAGCAGT GGAGTTTTTA TTCTGCTGAC 
GACTCAAACA AGAGCCTAAA AGAATTGGGG CTAAGAGATG GCAGCTCTAT TCTCTTGTTT 
GATACACGTT CGGATGGGGA TGTATATCTT GCATCAGAAA GACATTTATC TAAAGACGCG 
AAGCAGAACT TCCTCATGGT GCAGGACTGG TGCAGACAGC AGGATGAGAA AGAAACGGTT 
AAAATAATTA TCACTCCCAA CACTACTATA CAAGATATCC ATGTGAAAGC AGTCGAATTG 
CTGCAGCTAC AGGAAGAGTG TAGCAAAAAT GACACTTGCT TAAGACCCCT CCACAGAAAA 
GAGAAGCTTC TTCCCCCAGT GCCAGAAAGC CTAACTGTGC AGGAGGCAGA ACTGAAAAAT 
GGATGTACCT TAGGGCTCTG CAAAGGGAAA GCCCCCAGCC CCTCCCAGAT CTTCTTGTAT 
TTCATTTCCG GGACTGACCT GCAGGAGGGC AGCGAGCAGG AGATTATTTT AGAAGAAACT 
CTCACTGTGA GACAGAGCCT GGAAAGGATG CTGAATAAAG CAGGTTTGCC AGGTAAGGCA 
GGATGGCACC TGAGAAAGAT GGACTGGTGT TATGAAGCAG GAGAGGCTCT GAGCGATGAG 
GAGTCCACAC TGAAAGAGCT GAATATCTGC GCTGGAGACA CATTGGTGAT CACTGAAGGG 
AAACTTCCAC CCAAGGGTTT CCTGAAGCTT TCTATATGGC TGTACTGCCC CCATACACAG 
CAAGATGCTA TGAATCATGT GACCTGTGAA CCAGGTGCTC TACAGACCTC CACTACAGAA 
GATAATAACA TGCAAGAAGG TGGAACCTCT AATTTCTGCT CTGTTGGTGA GATAGAAATA 
TCAGGAGACT TATCATTACA GGATGTGAAA ATGCAGATCC TGACTTTGCC AGTTTTTGAA 
GCCCTAGGGA TCCCTTCTCC TGAGTTCCTC AGAGTCTGGA CCCTGGAAAA CAAGCATCTG 
GGGAAAATCC TGCGCTCGCC TCCAGTGTTA CTCAGTGAGT ACAAACTGGG GAGCAAGCCA 
GCACTATGCA TTGAGCCTTT GCAGGTGGAG GAATGTTTGG GTGCTAAAGA TCTTGTCTTG 
CGGGTGCAGC TGTCTGTCCC AGGGGAGTGT CGCTATTTTC CTCCCGTAGA TATTGTGTGG 
GATATATCTA GAGGCTGCAC AGCCCCAGCC TTGCTGCACA GAATTGCCAT GCATTGCTCC 
CTGCCAACAG ACAAGGTGGA AATTGCAAAG CACTTTCCAG AGAAATATGA GTGGCTTCCA 
ATATCCAGTT GGACCCAACA TGTCTCCAGA AAGAGGAAAA AGAAAAAGCT GGAAAGTCTA 
CAGGGGGCTC CTTATCACCT AAAGGATGGG GACATCATTG GGGTCAAGAA TCTTTTGCTT 
GATGACTGGA CAGACTTCAG CTCTGAAATT GACCGCATTG GAAAAGAAAA GCAGCAGCAG 
CAGCAGCAGC GCCCAGCGAA TGGAAACAAA AGTCTGCACG CAGAAGGATT GCAGAATAAT 
ACGGCTGGAA GCAATGGAAG GGCAAAGTCG AAGGCCCGGA AAGCGGAGAC CTCTCTCTCA 
ATCCGAGTGG GCGTATTCAG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp40 ( XM_012971226.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826680.1 Xenopus tropicalis ubiquitin specific peptidase 40 (usp40), transcript variant X3, mRNA.

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CloneID	OXa16185
Clone ID Related Accession (Same CDS sequence)	XM_002932005.4 , XM_012971227.2 , XM_012971226.2
Accession Version	XM_012971226.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3684bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ubiquitin carboxyl-terminal hydrolase 40
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012971226.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGGAG ACCTATTTGA GGAAGATGAA GACTTTTCAT TCCTTTTGAG CAGTCCAAAC 
ACGAATGGCA AGAAATCCCA CCCCAGAGAT TCAAAGACCC CAGAGCCCCG AGGAGACACA 
AGGCTTAGTG GACTGAGGAA TCAAGGTGGC ACCTGTTACC TAAATTCTCT TCTGCAGACC 
CTGTTTTTCA CACCAGAATT CAGAGAAGCC CTTTTTGCTT TGGGTCCTAA AGAACTTGGA 
TCACTGGAGG AGAAAGACAC GCCAGACTCT CGGGTGCGAA TTATTCCCCT GCAGTTACAG 
CGTCTCTTCG CTCAACTTCT GCTGCTGGAT CAGCAGGCCT TGTCAACCAC AGATCTCACA 
GAGAGCTTTG GGTGGAACAG TAGTGAGGAG ACAAGCCAGC ATGATGTGCA AGAGTTAAAC 
CGTATTTTGT TCAGTGCTCT TGAGAGCTCA TTGGAGGGCA CATCTGGCCA TGACCTCATC 
AAGCGATTGT ACCATGGCAC CATTGTTAAC TGTATCCAGT GCAAGGAGTG TGGCTACATC 
AGTGAAAGGC AGGAGGATTT CCTTGACTTG ACGGTGGCAG TGAAGGGCGT GGGCAGTCTG 
GAGAATTCTC TGTGTAGCAT GTATTTGGAA GAGGAACACT TTGATGGAGA TAATTTGTAC 
AGATGTGGGA GCTGTGCCAA GCTTGTCCCT GCAACCAAGT CTGCCAAGTT GGGGAAACTG 
CCACCCTTTC TCACACTATC TCTGCTGAGG TTTAACTTTG ACTTTGTAAA GTGTGAGCGT 
TATAAGGAAA CTTCACGATA CACCTTTCCC ACCCGCCTCA TCATCCGGCC CTTTTGTGAG 
CAGCATAATC TAGAAGACTC CGTATATGCC TATGAGCTCT TCTCTGTTAT TATACACAAG 
GGCGGCTGTT ATGGAGGTCA TTATCATGCA TATATTCAAG ATGTCGATGA GCTGGGGCAT 
TGGGGACTCA AGGAAGTGGC TAAAAAGACA GCATCAGCAC AGGAGTCTGA GAGTAAACAA 
GATTGCCCAA TAGTTGCTCT TGCTTCCATC ATAGCAGAGG CTGGGGCCGA TCAAATGGTG 
GCAGTTGACC AGCTGGGACA GAAGCTATTG GAATACATGG GAACATCTTG GAATAAGACT 
TATCGCAAGC AGCATGGAGC TATGCACAAG TTCCTGCAAA GCCATCCCTC CGTTTTCCAG 
ATGAGTGCAG ATGGCTCGCT TGTAGGTTTG GCGCATAGTG TTCCAGACAC AGTAGGCTCC 
GATTCAGAGA GCTCTAGGCC AGGTACTCCA GAGAAATCTC AAGCTCCATC TAGAAGTGCT 
GGTCATTGGT TTGATTTTAA CGATTCCAAA GTGGAACCCA TCCGCGAGAC GGATATTGAA 
AAGCAATTCC AGGGCAAGGA ATCTGCATAT ATGCTTTTTT ATCGCAAGTG TGAAATGAAT 
AGACCACCAG AAGCTAAAGG CAATCCTCGC TTTGGAGTCC CAGAGCACAT TCTAAATGAC 
ATGGACCAAG AAAATATCGA GCTTCAGACG AAAAGAACTG AATCGGCCTT ACAGATCAAC 
TGCATGGACC TTTGTCTCCA TCTGTCTTCT CATTACCAAT ATGAAAATGG TGCCCTGCAT 
CCAGCACATC CCCGAAACAA CAGTAAGCTG GCAATTGCTA TAGACCGCAG GAGGACTGTA 
GAAGATCTAA AACAATTAGC TTTCCAGGTT CTTGATGAAT CTTTGGAAGG ACAAGTCCTG 
AGTATTGCTA GAGTTCTACC TGCTGGACTT CATATCTTTC AGAATCTGAT TGATGCCAAG 
ATGGATCTGG TCACTGCAGG AATTACAGAT GGAACTGACA TCTTTGTGTG GGATGGAAAG 
CAGGTTGGGG GTGTAATGAT TCAGAGTGGA GCAGAGTTCA CGCCAGTCCT GCTCACCATC 
CTGCGCCCAG TCCTGCAAAC AAATTCTTCA GCTGAGTTCA GTGAGAGTCA GAGAGCGTTC 
CCATCATGTG CCTGCTTGAA AGACATACAA AAAGAGCTTG CTGGCCCCAC CTGTGAGGAG 
TCAGTGTTGT GCTTCCCTTC GTCAGAGAAT AGTGAGCAGT GGAGTTTTTA TTCTGCTGAC 
GACTCAAACA AGAGCCTAAA AGAATTGGGG CTAAGAGATG GCAGCTCTAT TCTCTTGTTT 
GATACACGTT CGGATGGGGA TGTATATCTT GCATCAGAAA GACATTTATC TAAAGACGCG 
AAGCAGAACT TCCTCATGGT GCAGGACTGG TGCAGACAGC AGGATGAGAA AGAAACGGTT 
AAAATAATTA TCACTCCCAA CACTACTATA CAAGATATCC ATGTGAAAGC AGTCGAATTG 
CTGCAGCTAC AGGAAGAGTG TAGCAAAAAT GACACTTGCT TAAGACCCCT CCACAGAAAA 
GAGAAGCTTC TTCCCCCAGT GCCAGAAAGC CTAACTGTGC AGGAGGCAGA ACTGAAAAAT 
GGATGTACCT TAGGGCTCTG CAAAGGGAAA GCCCCCAGCC CCTCCCAGAT CTTCTTGTAT 
TTCATTTCCG GGACTGACCT GCAGGAGGGC AGCGAGCAGG AGATTATTTT AGAAGAAACT 
CTCACTGTGA GACAGAGCCT GGAAAGGATG CTGAATAAAG CAGGTTTGCC AGGTAAGGCA 
GGATGGCACC TGAGAAAGAT GGACTGGTGT TATGAAGCAG GAGAGGCTCT GAGCGATGAG 
GAGTCCACAC TGAAAGAGCT GAATATCTGC GCTGGAGACA CATTGGTGAT CACTGAAGGG 
AAACTTCCAC CCAAGGGTTT CCTGAAGCTT TCTATATGGC TGTACTGCCC CCATACACAG 
CAAGATGCTA TGAATCATGT GACCTGTGAA CCAGGTGCTC TACAGACCTC CACTACAGAA 
GATAATAACA TGCAAGAAGG TGGAACCTCT AATTTCTGCT CTGTTGGTGA GATAGAAATA 
TCAGGAGACT TATCATTACA GGATGTGAAA ATGCAGATCC TGACTTTGCC AGTTTTTGAA 
GCCCTAGGGA TCCCTTCTCC TGAGTTCCTC AGAGTCTGGA CCCTGGAAAA CAAGCATCTG 
GGGAAAATCC TGCGCTCGCC TCCAGTGTTA CTCAGTGAGT ACAAACTGGG GAGCAAGCCA 
GCACTATGCA TTGAGCCTTT GCAGGTGGAG GAATGTTTGG GTGCTAAAGA TCTTGTCTTG 
CGGGTGCAGC TGTCTGTCCC AGGGGAGTGT CGCTATTTTC CTCCCGTAGA TATTGTGTGG 
GATATATCTA GAGGCTGCAC AGCCCCAGCC TTGCTGCACA GAATTGCCAT GCATTGCTCC 
CTGCCAACAG ACAAGGTGGA AATTGCAAAG CACTTTCCAG AGAAATATGA GTGGCTTCCA 
ATATCCAGTT GGACCCAACA TGTCTCCAGA AAGAGGAAAA AGAAAAAGCT GGAAAGTCTA 
CAGGGGGCTC CTTATCACCT AAAGGATGGG GACATCATTG GGGTCAAGAA TCTTTTGCTT 
GATGACTGGA CAGACTTCAG CTCTGAAATT GACCGCATTG GAAAAGAAAA GCAGCAGCAG 
CAGCAGCAGC GCCCAGCGAA TGGAAACAAA AGTCTGCACG CAGAAGGATT GCAGAATAAT 
ACGGCTGGAA GCAATGGAAG GGCAAAGTCG AAGGCCCGGA AAGCGGAGAC CTCTCTCTCA 
ATCCGAGTGG GCGTATTCAG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826680.1
CDS	1563..5246
Translation	MFGDLFEEDEDFSFLLSSPNTNGKKSHPRDSKTPEPRGDTRLSGLRNQGGTCYLNSLLQTLFFTPEFREALFALGPKELGSLEEKDTPDSRVRIIPLQLQRLFAQLLLLDQQALSTTDLTESFGWNSSEETSQHDVQELNRILFSALESSLEGTSGHDLIKRLYHGTIVNCIQCKECGYISERQEDFLDLTVAVKGVGSLENSLCSMYLEEEHFDGDNLYRCGSCAKLVPATKSAKLGKLPPFLTLSLLRFNFDFVKCERYKETSRYTFPTRLIIRPFCEQHNLEDSVYAYELFSVIIHKGGCYGGHYHAYIQDVDELGHWGLKEVAKKTASAQESESKQDCPIVALASIIAEAGADQMVAVDQLGQKLLEYMGTSWNKTYRKQHGAMHKFLQSHPSVFQMSADGSLVGLAHSVPDTVGSDSESSRPGTPEKSQAPSRSAGHWFDFNDSKVEPIRETDIEKQFQGKESAYMLFYRKCEMNRPPEAKGNPRFGVPEHILNDMDQENIELQTKRTESALQINCMDLCLHLSSHYQYENGALHPAHPRNNSKLAIAIDRRRTVEDLKQLAFQVLDESLEGQVLSIARVLPAGLHIFQNLIDAKMDLVTAGITDGTDIFVWDGKQVGGVMIQSGAEFTPVLLTILRPVLQTNSSAEFSESQRAFPSCACLKDIQKELAGPTCEESVLCFPSSENSEQWSFYSADDSNKSLKELGLRDGSSILLFDTRSDGDVYLASERHLSKDAKQNFLMVQDWCRQQDEKETVKIIITPNTTIQDIHVKAVELLQLQEECSKNDTCLRPLHRKEKLLPPVPESLTVQEAELKNGCTLGLCKGKAPSPSQIFLYFISGTDLQEGSEQEIILEETLTVRQSLERMLNKAGLPGKAGWHLRKMDWCYEAGEALSDEESTLKELNICAGDTLVITEGKLPPKGFLKLSIWLYCPHTQQDAMNHVTCEPGALQTSTTEDNNMQEGGTSNFCSVGEIEISGDLSLQDVKMQILTLPVFEALGIPSPEFLRVWTLENKHLGKILRSPPVLLSEYKLGSKPALCIEPLQVEECLGAKDLVLRVQLSVPGECRYFPPVDIVWDISRGCTAPALLHRIAMHCSLPTDKVEIAKHFPEKYEWLPISSWTQHVSRKRKKKKLESLQGAPYHLKDGDIIGVKNLLLDDWTDFSSEIDRIGKEKQQQQQQRPANGNKSLHAEGLQNNTAGSNGRAKSKARKAETSLSIRVGVFR

Target ORF information:

RefSeq Version	XM_012971226.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin specific peptidase 40 (usp40), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971226.2

ORF Insert Sequence:

ATGTTTGGAG ACCTATTTGA GGAAGATGAA GACTTTTCAT TCCTTTTGAG CAGTCCAAAC 
ACGAATGGCA AGAAATCCCA CCCCAGAGAT TCAAAGACCC CAGAGCCCCG AGGAGACACA 
AGGCTTAGTG GACTGAGGAA TCAAGGTGGC ACCTGTTACC TAAATTCTCT TCTGCAGACC 
CTGTTTTTCA CACCAGAATT CAGAGAAGCC CTTTTTGCTT TGGGTCCTAA AGAACTTGGA 
TCACTGGAGG AGAAAGACAC GCCAGACTCT CGGGTGCGAA TTATTCCCCT GCAGTTACAG 
CGTCTCTTCG CTCAACTTCT GCTGCTGGAT CAGCAGGCCT TGTCAACCAC AGATCTCACA 
GAGAGCTTTG GGTGGAACAG TAGTGAGGAG ACAAGCCAGC ATGATGTGCA AGAGTTAAAC 
CGTATTTTGT TCAGTGCTCT TGAGAGCTCA TTGGAGGGCA CATCTGGCCA TGACCTCATC 
AAGCGATTGT ACCATGGCAC CATTGTTAAC TGTATCCAGT GCAAGGAGTG TGGCTACATC 
AGTGAAAGGC AGGAGGATTT CCTTGACTTG ACGGTGGCAG TGAAGGGCGT GGGCAGTCTG 
GAGAATTCTC TGTGTAGCAT GTATTTGGAA GAGGAACACT TTGATGGAGA TAATTTGTAC 
AGATGTGGGA GCTGTGCCAA GCTTGTCCCT GCAACCAAGT CTGCCAAGTT GGGGAAACTG 
CCACCCTTTC TCACACTATC TCTGCTGAGG TTTAACTTTG ACTTTGTAAA GTGTGAGCGT 
TATAAGGAAA CTTCACGATA CACCTTTCCC ACCCGCCTCA TCATCCGGCC CTTTTGTGAG 
CAGCATAATC TAGAAGACTC CGTATATGCC TATGAGCTCT TCTCTGTTAT TATACACAAG 
GGCGGCTGTT ATGGAGGTCA TTATCATGCA TATATTCAAG ATGTCGATGA GCTGGGGCAT 
TGGGGACTCA AGGAAGTGGC TAAAAAGACA GCATCAGCAC AGGAGTCTGA GAGTAAACAA 
GATTGCCCAA TAGTTGCTCT TGCTTCCATC ATAGCAGAGG CTGGGGCCGA TCAAATGGTG 
GCAGTTGACC AGCTGGGACA GAAGCTATTG GAATACATGG GAACATCTTG GAATAAGACT 
TATCGCAAGC AGCATGGAGC TATGCACAAG TTCCTGCAAA GCCATCCCTC CGTTTTCCAG 
ATGAGTGCAG ATGGCTCGCT TGTAGGTTTG GCGCATAGTG TTCCAGACAC AGTAGGCTCC 
GATTCAGAGA GCTCTAGGCC AGGTACTCCA GAGAAATCTC AAGCTCCATC TAGAAGTGCT 
GGTCATTGGT TTGATTTTAA CGATTCCAAA GTGGAACCCA TCCGCGAGAC GGATATTGAA 
AAGCAATTCC AGGGCAAGGA ATCTGCATAT ATGCTTTTTT ATCGCAAGTG TGAAATGAAT 
AGACCACCAG AAGCTAAAGG CAATCCTCGC TTTGGAGTCC CAGAGCACAT TCTAAATGAC 
ATGGACCAAG AAAATATCGA GCTTCAGACG AAAAGAACTG AATCGGCCTT ACAGATCAAC 
TGCATGGACC TTTGTCTCCA TCTGTCTTCT CATTACCAAT ATGAAAATGG TGCCCTGCAT 
CCAGCACATC CCCGAAACAA CAGTAAGCTG GCAATTGCTA TAGACCGCAG GAGGACTGTA 
GAAGATCTAA AACAATTAGC TTTCCAGGTT CTTGATGAAT CTTTGGAAGG ACAAGTCCTG 
AGTATTGCTA GAGTTCTACC TGCTGGACTT CATATCTTTC AGAATCTGAT TGATGCCAAG 
ATGGATCTGG TCACTGCAGG AATTACAGAT GGAACTGACA TCTTTGTGTG GGATGGAAAG 
CAGGTTGGGG GTGTAATGAT TCAGAGTGGA GCAGAGTTCA CGCCAGTCCT GCTCACCATC 
CTGCGCCCAG TCCTGCAAAC AAATTCTTCA GCTGAGTTCA GTGAGAGTCA GAGAGCGTTC 
CCATCATGTG CCTGCTTGAA AGACATACAA AAAGAGCTTG CTGGCCCCAC CTGTGAGGAG 
TCAGTGTTGT GCTTCCCTTC GTCAGAGAAT AGTGAGCAGT GGAGTTTTTA TTCTGCTGAC 
GACTCAAACA AGAGCCTAAA AGAATTGGGG CTAAGAGATG GCAGCTCTAT TCTCTTGTTT 
GATACACGTT CGGATGGGGA TGTATATCTT GCATCAGAAA GACATTTATC TAAAGACGCG 
AAGCAGAACT TCCTCATGGT GCAGGACTGG TGCAGACAGC AGGATGAGAA AGAAACGGTT 
AAAATAATTA TCACTCCCAA CACTACTATA CAAGATATCC ATGTGAAAGC AGTCGAATTG 
CTGCAGCTAC AGGAAGAGTG TAGCAAAAAT GACACTTGCT TAAGACCCCT CCACAGAAAA 
GAGAAGCTTC TTCCCCCAGT GCCAGAAAGC CTAACTGTGC AGGAGGCAGA ACTGAAAAAT 
GGATGTACCT TAGGGCTCTG CAAAGGGAAA GCCCCCAGCC CCTCCCAGAT CTTCTTGTAT 
TTCATTTCCG GGACTGACCT GCAGGAGGGC AGCGAGCAGG AGATTATTTT AGAAGAAACT 
CTCACTGTGA GACAGAGCCT GGAAAGGATG CTGAATAAAG CAGGTTTGCC AGGTAAGGCA 
GGATGGCACC TGAGAAAGAT GGACTGGTGT TATGAAGCAG GAGAGGCTCT GAGCGATGAG 
GAGTCCACAC TGAAAGAGCT GAATATCTGC GCTGGAGACA CATTGGTGAT CACTGAAGGG 
AAACTTCCAC CCAAGGGTTT CCTGAAGCTT TCTATATGGC TGTACTGCCC CCATACACAG 
CAAGATGCTA TGAATCATGT GACCTGTGAA CCAGGTGCTC TACAGACCTC CACTACAGAA 
GATAATAACA TGCAAGAAGG TGGAACCTCT AATTTCTGCT CTGTTGGTGA GATAGAAATA 
TCAGGAGACT TATCATTACA GGATGTGAAA ATGCAGATCC TGACTTTGCC AGTTTTTGAA 
GCCCTAGGGA TCCCTTCTCC TGAGTTCCTC AGAGTCTGGA CCCTGGAAAA CAAGCATCTG 
GGGAAAATCC TGCGCTCGCC TCCAGTGTTA CTCAGTGAGT ACAAACTGGG GAGCAAGCCA 
GCACTATGCA TTGAGCCTTT GCAGGTGGAG GAATGTTTGG GTGCTAAAGA TCTTGTCTTG 
CGGGTGCAGC TGTCTGTCCC AGGGGAGTGT CGCTATTTTC CTCCCGTAGA TATTGTGTGG 
GATATATCTA GAGGCTGCAC AGCCCCAGCC TTGCTGCACA GAATTGCCAT GCATTGCTCC 
CTGCCAACAG ACAAGGTGGA AATTGCAAAG CACTTTCCAG AGAAATATGA GTGGCTTCCA 
ATATCCAGTT GGACCCAACA TGTCTCCAGA AAGAGGAAAA AGAAAAAGCT GGAAAGTCTA 
CAGGGGGCTC CTTATCACCT AAAGGATGGG GACATCATTG GGGTCAAGAA TCTTTTGCTT 
GATGACTGGA CAGACTTCAG CTCTGAAATT GACCGCATTG GAAAAGAAAA GCAGCAGCAG 
CAGCAGCAGC GCCCAGCGAA TGGAAACAAA AGTCTGCACG CAGAAGGATT GCAGAATAAT 
ACGGCTGGAA GCAATGGAAG GGCAAAGTCG AAGGCCCGGA AAGCGGAGAC CTCTCTCTCA 
ATCCGAGTGG GCGTATTCAG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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usp40 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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