nfasc cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following nfasc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nfasc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa03809 XM_004910696.4
Latest version!
Xenopus tropicalis neurofascin (nfasc), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa03810 XM_004910697.4
Latest version!
Xenopus tropicalis neurofascin (nfasc), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa03812 XM_031896834.1
Latest version!
Xenopus tropicalis neurofascin (nfasc), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa03813 XM_031896836.1
Latest version!
Xenopus tropicalis neurofascin (nfasc), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa03814 XM_031896839.1
Latest version!
Xenopus tropicalis neurofascin (nfasc), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa03815 XM_031896840.1
Latest version!
Xenopus tropicalis neurofascin (nfasc), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa03816 XM_031896841.1
Latest version!
Xenopus tropicalis neurofascin (nfasc), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa33490 XM_031896830.1
Latest version!
Xenopus tropicalis neurofascin (nfasc), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa33491 XM_031896831.1
Latest version!
Xenopus tropicalis neurofascin (nfasc), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa33493 XM_031896835.1
Latest version!
Xenopus tropicalis neurofascin (nfasc), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa52226 XM_031896832.1
Latest version!
Xenopus tropicalis neurofascin (nfasc), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa52227 XM_031896833.1
Latest version!
Xenopus tropicalis neurofascin (nfasc), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa52228 XM_031896837.1
Latest version!
Xenopus tropicalis neurofascin (nfasc), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa52229 XM_031896838.1
Latest version!
Xenopus tropicalis neurofascin (nfasc), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa52230 XM_031896842.1
Latest version!
Xenopus tropicalis neurofascin (nfasc), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa42746 XM_018091565.1
Latest version!
Xenopus tropicalis neurofascin (nfasc), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OXa42747 XM_018091566.1
Latest version!
Xenopus tropicalis neurofascin (nfasc), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
View Old Accession Versions >>
OXa03809 XM_004910696.3 Xenopus tropicalis neurofascin (nfasc), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa03810 XM_004910697.3 Xenopus tropicalis neurofascin (nfasc), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa03809
    Clone ID Related Accession (Same CDS sequence) XM_004910696.4 , XM_004910696.3
    Accession Version XM_004910696.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4017bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neurofascin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004910696.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGACAGATT TTCTAGAAAG GAGGTTAACG GTGATCCACT ACTACTGTCT CGGAAGCAAC 
    ATGCCATCAT GTTTAGTCCT TCTCCTGCTC TTTCTCCACC TAGTCTGCAC TATTAAAGTT
    CCACTGGACC CAAACATTCA GCGAGACCTA ATACAGCCCC CAACCATCAT CAAGCAGTCA
    GCAAAGAATT ATATTGTAGA TCCCCGTGAT AACATCCTAA TTGAGTGTGA AGCTCAGGGG
    AATCCTTACC CAATGTTTTC ATGGACAAAA AATGGGAAAT TTTACAATGT GGCAAAAGAC
    CCTCGAGTGT CATTCCGTCG ACGATCAGGC ACCCTTGAAA TAGAAATTCA TGGAGGAGGG
    CGGCCAGAGG ACTATGAGGG AGAATACCAA TGCTCTGCCC GCAACCAACA TGGAACAGCT
    TTATCAAATA AGATCTTACT TCAAGTCTCT CGATCCCCTT TGTGGCCCAA AGAAAACCTT
    GACCCGGTGG TGGTAAATGA AGGTGCCTTC TTGATCCTTC AGTGCAATCC CCCACATGGA
    CTTCCTCCTC CAGTCATATT TTGGATGAGC AGCTTGATGG AGCCTATTGT CCAGGACAAG
    AGGGTTTCTC AGGGACTGAA TGGGGACCTT TATTTCTCCA ACGTTCTGCG CCAGGATACT
    CAGACTGATT ATAGCTGCAA TGCACGCTCC CATTTCACAC ACACCATTCA ACAGAAGAAC
    CCATATACTC TAAAAGTGCT TACCACCAGA GGGGTACCTG AGAGAACCCC AACCTTCTTG
    CTTCCCACGG GGACTTCTAG CAGTAAACTG GTGTTACGCG ATGAGGACTT GCTACTGGAG
    TGTATTGCTT CTGGAGTACC AACGCCAGAC ATCATGTGGT ATAAGAAAGG AGGAGAGCTA
    CCCAGCAGGA AGCTGAAGTT TGAAAATTTT AACAAGACTC TACGCATAAT TGGAGTGTCT
    GAAGAGGACT CGGGGGAGTA TTTCTGCCAA GCTTCAAACA AAATGGGCAG CATTCGGCAC
    ACGATTTCTG TTCGGGTTAA GGCTGCTCCA TATTGGCTTG ATGAGCCCAA GAACTTGATT
    CTTGCACCAG GTGAGAACGG ACGTTTGGTA TGTCGTGCCA GTGGCAGCCC CAAACCTACA
    ATCCAATGGT TTATGAATGG AGATGCTACT GAAGTAGGTG CCCCAAATCC CAACTTCGAA
    ATCGCTGGTG ATACCATTAT TTTTAGAGAA GTACAGAGTG GCAACAGCGC TGTATACCAA
    TGCAATGCTT CTAATGAACA TGGTTATCTG TTAGCTAATG CCTTCGTCAG CATTCTGGAT
    GTTCCATCTC GGATGTTGGG CCCTCGTAAT CAGCTGATTC GAGTGATAGA ATACAACAGG
    ACCCAGCTAG ACTGCCCTTT CTTTGGCTCT CCTATACCTA CACTCCGCTG GTTTAAAAAT
    GGACAGGGTA GTAACCTCGA TGGTGGCAAT TACCGGGTGC ATGAAAATGG GACCCTTGAG
    ATTAATACTG TGAGGAAGGA GGACCAAGGC AAATACACAT GTGTTGCTAC CAATATTTTG
    GGCAATGCTG AAAATACTGT GCGGCTGGAG GTCAAAGAGC CAACTCGTAT TGTAAAGGGC
    CCTGAAGATC AGATTGCGAA GAGGGGCTCT TCTGTGCATC TGGACTGTCG AGTGCGCAGT
    GACTCCTCAC TCAGACTCAC TGTTACCTGG ATAAAAGATG ACACTCAGTT GTATATAAAC
    AGCAGAATGA AGAAGGATGA GGATGGACTT ACAATATTCG GTGTGGCAGA AAAGGATCAG
    GGAGCATATA CATGTGTTGC TAAAACTGAA TTAGACCAGG ACTCTTCTAA AGCTTATCTC
    ACGATACAGG AAACTTCCTC ACCTACTAGC CGACTGACCT CCCAGCTTAT AGACAAACCA
    GACCCCCCAC AGGACCTGGA GCTAACTGAA CCATCAGTTA GGAGTGTTCG CCTCACCTGG
    GTTCCAGGGG ATGATAACAA CAGTCCAATT ACAGGTTTCA TTGTACAATT TGAGGAAGAC
    AGGCATCAGC CCGGTACATG GCACAACCTT GCCTCTGTCC CTGCTAATGT GAACTCTGTC
    ATACTGCAGC TTTCTCCTTA TGTCAACTAC CAGTTTCGTG TTCTTGCACT AAATGAACTT
    GGGAGCAGTA TGCCAAGCCC ATCTTCAGAA CGTTTTCAGA CAGCAGGAGC ACCTCCAGAA
    ACTAATCCAG GTGATGTGAA GGGAGCAGGG ACTAGCAAAA ACAATATGGA AATCACATGG
    ACACCACTGA ACTCCACTCA GGCGTATGGT CCAAACCTGC GCTACAACGT GAAGTGGCGC
    CAACGTCACA CAGGAGAACC TTGGAGCAAT ATTACAGTGT GGGACAACAA GCATGTGGTG
    TGGAATACAC CAGTATACAC CCAATATGAG CTAAGAGTTC AGGCGGAAAA TGAATTTGGA
    AAGGCTCCAG AGCCAAGCAC CATGATTGGA TATTCTGGTG AAGACTATCC TAAGGCTGCA
    CCCACTGACA TTAAGCTTCG GGTCATGAAC AGCACCGCCA TCTCTCTTCA GTGGACACGT
    GTGGCACCTG CCTCTATCCA AGGAAGCCTA CTGGAATACA GAGCTTATTT TTGGAGAGAA
    AGTAGTTTGC TGAAGGGTGT CTGGGTCTCA CGAAAGAAGC AACAACAGAG TTTTATGGGG
    GACCGTGTGA GAGGCATCGT ATCTCAACTG ATTCCATACA GCATCTACAA GCTGTACTTG
    GTGGCAGTGA ATGGCCGAGG GGATGGCCCC AAAAGTGATG TTAAGGAATT CACAACCCCA
    GAGGGAGTGC CGAGTGCTCC TAAATACTTT AGAGTATATG AGCCAAACCG GGAAATGATT
    GTTGTTGAAT GGGGTCACCC AGAGCACCCG AACGGAAACT TGACTGCATA CAACCTCCGC
    ATCCAACCAT TTAATGGCAC CAGAACAGGG AAGGCTGTAT TTGAAACTCT TTCACGTAAT
    CAAACCAAAT ATACCCTGCA AAGAACAGAT CCCGTGTCCC GGTATCGGTT TGCTATCAAT
    GCCAGAACGC AGGTTGGTGC AGGGGAAGTC ATCACAGCTG AGTCTCCTGT CAGACTGGAT
    GAAGTTTCTC CCACGTCAGT GTCTTCTAAG TCTCCTGCAC CTGTCACAGA TGCTCCCACA
    GAGGTCCCAA CTATTCCCCC AACCTCATCT GGTGCCACCA CTCTTCTAGC TACAACCAGT
    ACAACCAGTG TCACAACGAC TACTGCTGGT ACAGAAAGTT CGCTGGTCGC CATTACTAGA
    ATTCCTAATA TAGCCACCAC TGGACCATCT CTATGGAACC TAACAGCATT ACCCAACACT
    TACTGGGCAA ACATCACCTG GATGCATAAC TTAGAGCCAG GCTCTGTGTT TATGGTTGAG
    TATACTGACA GTAATAATAC AGTGAAAACT GTTCCTGTTA AGGCTCATGT CCCTTCTATC
    CAACTGTTTG ATCTGGTGCC TGGGATGACC TACAGGGTGC GGGTTTACTC CCAGGAGCAC
    CCGTCAGTCA CAAGCCTATA TGTGACATTT GATACCAGCT CAGCTTACAC CAAGAACCAA
    GTTGACATTG CTACACAAGG CTGGTTTATT GGGCTCATGT GTGCAGTTGC TCTACTGGTA
    CTGATTTTGC TGATCGTTTG TTTTATCAAA AGAAGCCGAG GAGGCAAATA CCCAGTGCGA
    GAAAAGAAGG AGGTGGCGTT GGGACCAGAT GATAACAAAG AGGGGGATGG CTCATTTGAC
    TACAGTGATG AGGACAACAA GCCCCTGCAG GGTAGTCAAA CTTCGCTGGA TGGAACCATA
    AAGCAACAAG AGAGTGATGA CAGCCTTGTT GATTATGGAG AAGGAGGAGA GGGGCAGTTT
    AATGAAGATG GATCGTTTAT AGGGCAGTAT ACAGTGAAAA AGGACAAAGA TGAGACTGAG
    GGAAATGAAA GTTCAGAGGC CACATCTCCT GTAAATGCTA TCTATTCTCT GGCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004910753.1
    CDS547..4563
    Translation

    Target ORF information:

    RefSeq Version XM_004910696.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neurofascin (nfasc), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004910696.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGACAGATT TTCTAGAAAG GAGGTTAACG GTGATCCACT ACTACTGTCT CGGAAGCAAC 
    ATGCCATCAT GTTTAGTCCT TCTCCTGCTC TTTCTCCACC TAGTCTGCAC TATTAAAGTT
    CCACTGGACC CAAACATTCA GCGAGACCTA ATACAGCCCC CAACCATCAT CAAGCAGTCA
    GCAAAGAATT ATATTGTAGA TCCCCGTGAT AACATCCTAA TTGAGTGTGA AGCTCAGGGG
    AATCCTTACC CAATGTTTTC ATGGACAAAA AATGGGAAAT TTTACAATGT GGCAAAAGAC
    CCTCGAGTGT CATTCCGTCG ACGATCAGGC ACCCTTGAAA TAGAAATTCA TGGAGGAGGG
    CGGCCAGAGG ACTATGAGGG AGAATACCAA TGCTCTGCCC GCAACCAACA TGGAACAGCT
    TTATCAAATA AGATCTTACT TCAAGTCTCT CGATCCCCTT TGTGGCCCAA AGAAAACCTT
    GACCCGGTGG TGGTAAATGA AGGTGCCTTC TTGATCCTTC AGTGCAATCC CCCACATGGA
    CTTCCTCCTC CAGTCATATT TTGGATGAGC AGCTTGATGG AGCCTATTGT CCAGGACAAG
    AGGGTTTCTC AGGGACTGAA TGGGGACCTT TATTTCTCCA ACGTTCTGCG CCAGGATACT
    CAGACTGATT ATAGCTGCAA TGCACGCTCC CATTTCACAC ACACCATTCA ACAGAAGAAC
    CCATATACTC TAAAAGTGCT TACCACCAGA GGGGTACCTG AGAGAACCCC AACCTTCTTG
    CTTCCCACGG GGACTTCTAG CAGTAAACTG GTGTTACGCG ATGAGGACTT GCTACTGGAG
    TGTATTGCTT CTGGAGTACC AACGCCAGAC ATCATGTGGT ATAAGAAAGG AGGAGAGCTA
    CCCAGCAGGA AGCTGAAGTT TGAAAATTTT AACAAGACTC TACGCATAAT TGGAGTGTCT
    GAAGAGGACT CGGGGGAGTA TTTCTGCCAA GCTTCAAACA AAATGGGCAG CATTCGGCAC
    ACGATTTCTG TTCGGGTTAA GGCTGCTCCA TATTGGCTTG ATGAGCCCAA GAACTTGATT
    CTTGCACCAG GTGAGAACGG ACGTTTGGTA TGTCGTGCCA GTGGCAGCCC CAAACCTACA
    ATCCAATGGT TTATGAATGG AGATGCTACT GAAGTAGGTG CCCCAAATCC CAACTTCGAA
    ATCGCTGGTG ATACCATTAT TTTTAGAGAA GTACAGAGTG GCAACAGCGC TGTATACCAA
    TGCAATGCTT CTAATGAACA TGGTTATCTG TTAGCTAATG CCTTCGTCAG CATTCTGGAT
    GTTCCATCTC GGATGTTGGG CCCTCGTAAT CAGCTGATTC GAGTGATAGA ATACAACAGG
    ACCCAGCTAG ACTGCCCTTT CTTTGGCTCT CCTATACCTA CACTCCGCTG GTTTAAAAAT
    GGACAGGGTA GTAACCTCGA TGGTGGCAAT TACCGGGTGC ATGAAAATGG GACCCTTGAG
    ATTAATACTG TGAGGAAGGA GGACCAAGGC AAATACACAT GTGTTGCTAC CAATATTTTG
    GGCAATGCTG AAAATACTGT GCGGCTGGAG GTCAAAGAGC CAACTCGTAT TGTAAAGGGC
    CCTGAAGATC AGATTGCGAA GAGGGGCTCT TCTGTGCATC TGGACTGTCG AGTGCGCAGT
    GACTCCTCAC TCAGACTCAC TGTTACCTGG ATAAAAGATG ACACTCAGTT GTATATAAAC
    AGCAGAATGA AGAAGGATGA GGATGGACTT ACAATATTCG GTGTGGCAGA AAAGGATCAG
    GGAGCATATA CATGTGTTGC TAAAACTGAA TTAGACCAGG ACTCTTCTAA AGCTTATCTC
    ACGATACAGG AAACTTCCTC ACCTACTAGC CGACTGACCT CCCAGCTTAT AGACAAACCA
    GACCCCCCAC AGGACCTGGA GCTAACTGAA CCATCAGTTA GGAGTGTTCG CCTCACCTGG
    GTTCCAGGGG ATGATAACAA CAGTCCAATT ACAGGTTTCA TTGTACAATT TGAGGAAGAC
    AGGCATCAGC CCGGTACATG GCACAACCTT GCCTCTGTCC CTGCTAATGT GAACTCTGTC
    ATACTGCAGC TTTCTCCTTA TGTCAACTAC CAGTTTCGTG TTCTTGCACT AAATGAACTT
    GGGAGCAGTA TGCCAAGCCC ATCTTCAGAA CGTTTTCAGA CAGCAGGAGC ACCTCCAGAA
    ACTAATCCAG GTGATGTGAA GGGAGCAGGG ACTAGCAAAA ACAATATGGA AATCACATGG
    ACACCACTGA ACTCCACTCA GGCGTATGGT CCAAACCTGC GCTACAACGT GAAGTGGCGC
    CAACGTCACA CAGGAGAACC TTGGAGCAAT ATTACAGTGT GGGACAACAA GCATGTGGTG
    TGGAATACAC CAGTATACAC CCAATATGAG CTAAGAGTTC AGGCGGAAAA TGAATTTGGA
    AAGGCTCCAG AGCCAAGCAC CATGATTGGA TATTCTGGTG AAGACTATCC TAAGGCTGCA
    CCCACTGACA TTAAGCTTCG GGTCATGAAC AGCACCGCCA TCTCTCTTCA GTGGACACGT
    GTGGCACCTG CCTCTATCCA AGGAAGCCTA CTGGAATACA GAGCTTATTT TTGGAGAGAA
    AGTAGTTTGC TGAAGGGTGT CTGGGTCTCA CGAAAGAAGC AACAACAGAG TTTTATGGGG
    GACCGTGTGA GAGGCATCGT ATCTCAACTG ATTCCATACA GCATCTACAA GCTGTACTTG
    GTGGCAGTGA ATGGCCGAGG GGATGGCCCC AAAAGTGATG TTAAGGAATT CACAACCCCA
    GAGGGAGTGC CGAGTGCTCC TAAATACTTT AGAGTATATG AGCCAAACCG GGAAATGATT
    GTTGTTGAAT GGGGTCACCC AGAGCACCCG AACGGAAACT TGACTGCATA CAACCTCCGC
    ATCCAACCAT TTAATGGCAC CAGAACAGGG AAGGCTGTAT TTGAAACTCT TTCACGTAAT
    CAAACCAAAT ATACCCTGCA AAGAACAGAT CCCGTGTCCC GGTATCGGTT TGCTATCAAT
    GCCAGAACGC AGGTTGGTGC AGGGGAAGTC ATCACAGCTG AGTCTCCTGT CAGACTGGAT
    GAAGTTTCTC CCACGTCAGT GTCTTCTAAG TCTCCTGCAC CTGTCACAGA TGCTCCCACA
    GAGGTCCCAA CTATTCCCCC AACCTCATCT GGTGCCACCA CTCTTCTAGC TACAACCAGT
    ACAACCAGTG TCACAACGAC TACTGCTGGT ACAGAAAGTT CGCTGGTCGC CATTACTAGA
    ATTCCTAATA TAGCCACCAC TGGACCATCT CTATGGAACC TAACAGCATT ACCCAACACT
    TACTGGGCAA ACATCACCTG GATGCATAAC TTAGAGCCAG GCTCTGTGTT TATGGTTGAG
    TATACTGACA GTAATAATAC AGTGAAAACT GTTCCTGTTA AGGCTCATGT CCCTTCTATC
    CAACTGTTTG ATCTGGTGCC TGGGATGACC TACAGGGTGC GGGTTTACTC CCAGGAGCAC
    CCGTCAGTCA CAAGCCTATA TGTGACATTT GATACCAGCT CAGCTTACAC CAAGAACCAA
    GTTGACATTG CTACACAAGG CTGGTTTATT GGGCTCATGT GTGCAGTTGC TCTACTGGTA
    CTGATTTTGC TGATCGTTTG TTTTATCAAA AGAAGCCGAG GAGGCAAATA CCCAGTGCGA
    GAAAAGAAGG AGGTGGCGTT GGGACCAGAT GATAACAAAG AGGGGGATGG CTCATTTGAC
    TACAGTGATG AGGACAACAA GCCCCTGCAG GGTAGTCAAA CTTCGCTGGA TGGAACCATA
    AAGCAACAAG AGAGTGATGA CAGCCTTGTT GATTATGGAG AAGGAGGAGA GGGGCAGTTT
    AATGAAGATG GATCGTTTAT AGGGCAGTAT ACAGTGAAAA AGGACAAAGA TGAGACTGAG
    GGAAATGAAA GTTCAGAGGC CACATCTCCT GTAAATGCTA TCTATTCTCT GGCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa03810
    Clone ID Related Accession (Same CDS sequence) XM_004910697.4 , XM_004910697.3
    Accession Version XM_004910697.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3999bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neurofascin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004910697.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGACAGATT TTCTAGAAAG GAGGTTAACG GTGATCCACT ACTACTGTCT CGGAAGCAAC 
    ATGCCATCAT GTTTAGTCCT TCTCCTGCTC TTTCTCCACC TAGTCTGCAC TATTAAAGTT
    CCACTGGACC TAATACAGCC CCCAACCATC ATCAAGCAGT CAGCAAAGAA TTATATTGTA
    GATCCCCGTG ATAACATCCT AATTGAGTGT GAAGCTCAGG GGAATCCTTA CCCAATGTTT
    TCATGGACAA AAAATGGGAA ATTTTACAAT GTGGCAAAAG ACCCTCGAGT GTCATTCCGT
    CGACGATCAG GCACCCTTGA AATAGAAATT CATGGAGGAG GGCGGCCAGA GGACTATGAG
    GGAGAATACC AATGCTCTGC CCGCAACCAA CATGGAACAG CTTTATCAAA TAAGATCTTA
    CTTCAAGTCT CTCGATCCCC TTTGTGGCCC AAAGAAAACC TTGACCCGGT GGTGGTAAAT
    GAAGGTGCCT TCTTGATCCT TCAGTGCAAT CCCCCACATG GACTTCCTCC TCCAGTCATA
    TTTTGGATGA GCAGCTTGAT GGAGCCTATT GTCCAGGACA AGAGGGTTTC TCAGGGACTG
    AATGGGGACC TTTATTTCTC CAACGTTCTG CGCCAGGATA CTCAGACTGA TTATAGCTGC
    AATGCACGCT CCCATTTCAC ACACACCATT CAACAGAAGA ACCCATATAC TCTAAAAGTG
    CTTACCACCA GAGGGGTACC TGAGAGAACC CCAACCTTCT TGCTTCCCAC GGGGACTTCT
    AGCAGTAAAC TGGTGTTACG CGATGAGGAC TTGCTACTGG AGTGTATTGC TTCTGGAGTA
    CCAACGCCAG ACATCATGTG GTATAAGAAA GGAGGAGAGC TACCCAGCAG GAAGCTGAAG
    TTTGAAAATT TTAACAAGAC TCTACGCATA ATTGGAGTGT CTGAAGAGGA CTCGGGGGAG
    TATTTCTGCC AAGCTTCAAA CAAAATGGGC AGCATTCGGC ACACGATTTC TGTTCGGGTT
    AAGGCTGCTC CATATTGGCT TGATGAGCCC AAGAACTTGA TTCTTGCACC AGGTGAGAAC
    GGACGTTTGG TATGTCGTGC CAGTGGCAGC CCCAAACCTA CAATCCAATG GTTTATGAAT
    GGAGATGCTA CTGAAGTAGG TGCCCCAAAT CCCAACTTCG AAATCGCTGG TGATACCATT
    ATTTTTAGAG AAGTACAGAG TGGCAACAGC GCTGTATACC AATGCAATGC TTCTAATGAA
    CATGGTTATC TGTTAGCTAA TGCCTTCGTC AGCATTCTGG ATGTTCCATC TCGGATGTTG
    GGCCCTCGTA ATCAGCTGAT TCGAGTGATA GAATACAACA GGACCCAGCT AGACTGCCCT
    TTCTTTGGCT CTCCTATACC TACACTCCGC TGGTTTAAAA ATGGACAGGG TAGTAACCTC
    GATGGTGGCA ATTACCGGGT GCATGAAAAT GGGACCCTTG AGATTAATAC TGTGAGGAAG
    GAGGACCAAG GCAAATACAC ATGTGTTGCT ACCAATATTT TGGGCAATGC TGAAAATACT
    GTGCGGCTGG AGGTCAAAGA GCCAACTCGT ATTGTAAAGG GCCCTGAAGA TCAGATTGCG
    AAGAGGGGCT CTTCTGTGCA TCTGGACTGT CGAGTGCGCA GTGACTCCTC ACTCAGACTC
    ACTGTTACCT GGATAAAAGA TGACACTCAG TTGTATATAA ACAGCAGAAT GAAGAAGGAT
    GAGGATGGAC TTACAATATT CGGTGTGGCA GAAAAGGATC AGGGAGCATA TACATGTGTT
    GCTAAAACTG AATTAGACCA GGACTCTTCT AAAGCTTATC TCACGATACA GGAAACTTCC
    TCACCTACTA GCCGACTGAC CTCCCAGCTT ATAGACAAAC CAGACCCCCC ACAGGACCTG
    GAGCTAACTG AACCATCAGT TAGGAGTGTT CGCCTCACCT GGGTTCCAGG GGATGATAAC
    AACAGTCCAA TTACAGGTTT CATTGTACAA TTTGAGGAAG ACAGGCATCA GCCCGGTACA
    TGGCACAACC TTGCCTCTGT CCCTGCTAAT GTGAACTCTG TCATACTGCA GCTTTCTCCT
    TATGTCAACT ACCAGTTTCG TGTTCTTGCA CTAAATGAAC TTGGGAGCAG TATGCCAAGC
    CCATCTTCAG AACGTTTTCA GACAGCAGGA GCACCTCCAG AAACTAATCC AGGTGATGTG
    AAGGGAGCAG GGACTAGCAA AAACAATATG GAAATCACAT GGACACCACT GAACTCCACT
    CAGGCGTATG GTCCAAACCT GCGCTACAAC GTGAAGTGGC GCCAACGTCA CACAGGAGAA
    CCTTGGAGCA ATATTACAGT GTGGGACAAC AAGCATGTGG TGTGGAATAC ACCAGTATAC
    ACCCAATATG AGCTAAGAGT TCAGGCGGAA AATGAATTTG GAAAGGCTCC AGAGCCAAGC
    ACCATGATTG GATATTCTGG TGAAGACTAT CCTAAGGCTG CACCCACTGA CATTAAGCTT
    CGGGTCATGA ACAGCACCGC CATCTCTCTT CAGTGGACAC GTGTGGCACC TGCCTCTATC
    CAAGGAAGCC TACTGGAATA CAGAGCTTAT TTTTGGAGAG AAAGTAGTTT GCTGAAGGGT
    GTCTGGGTCT CACGAAAGAA GCAACAACAG AGTTTTATGG GGGACCGTGT GAGAGGCATC
    GTATCTCAAC TGATTCCATA CAGCATCTAC AAGCTGTACT TGGTGGCAGT GAATGGCCGA
    GGGGATGGCC CCAAAAGTGA TGTTAAGGAA TTCACAACCC CAGAGGGAGT GCCGAGTGCT
    CCTAAATACT TTAGAGTATA TGAGCCAAAC CGGGAAATGA TTGTTGTTGA ATGGGGTCAC
    CCAGAGCACC CGAACGGAAA CTTGACTGCA TACAACCTCC GCATCCAACC ATTTAATGGC
    ACCAGAACAG GGAAGGCTGT ATTTGAAACT CTTTCACGTA ATCAAACCAA ATATACCCTG
    CAAAGAACAG ATCCCGTGTC CCGGTATCGG TTTGCTATCA ATGCCAGAAC GCAGGTTGGT
    GCAGGGGAAG TCATCACAGC TGAGTCTCCT GTCAGACTGG ATGAAGTTTC TCCCACGTCA
    GTGTCTTCTA AGTCTCCTGC ACCTGTCACA GATGCTCCCA CAGAGGTCCC AACTATTCCC
    CCAACCTCAT CTGGTGCCAC CACTCTTCTA GCTACAACCA GTACAACCAG TGTCACAACG
    ACTACTGCTG GTACAGAAAG TTCGCTGGTC GCCATTACTA GAATTCCTAA TATAGCCACC
    ACTGGACCAT CTCTATGGAA CCTAACAGCA TTACCCAACA CTTACTGGGC AAACATCACC
    TGGATGCATA ACTTAGAGCC AGGCTCTGTG TTTATGGTTG AGTATACTGA CAGTAATAAT
    ACAGTGAAAA CTGTTCCTGT TAAGGCTCAT GTCCCTTCTA TCCAACTGTT TGATCTGGTG
    CCTGGGATGA CCTACAGGGT GCGGGTTTAC TCCCAGGAGC ACCCGTCAGT CACAAGCCTA
    TATGTGACAT TTGATACCAG CTCAGCTTAC ACCAAGAACC AAGTTGACAT TGCTACACAA
    GGCTGGTTTA TTGGGCTCAT GTGTGCAGTT GCTCTACTGG TACTGATTTT GCTGATCGTT
    TGTTTTATCA AAAGAAGCCG AGGAGGCAAA TACCCAGTGC GAGAAAAGAA GGAGGTGGCG
    TTGGGACCAG ATGATAACAA AGAGGGGGAT GGCTCATTTG ACTACAGTGA TGAGGACAAC
    AAGCCCCTGC AGGGTAGTCA AACTTCGCTG GATGGAACCA TAAAGCAACA AGAGAGTGAT
    GACAGCCTTG TTGATTATGG AGAAGGAGGA GAGGGGCAGT TTAATGAAGA TGGATCGTTT
    ATAGGGCAGT ATACAGTGAA AAAGGACAAA GATGAGACTG AGGGAAATGA AAGTTCAGAG
    GCCACATCTC CTGTAAATGC TATCTATTCT CTGGCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004910754.1
    CDS547..4545
    Translation

    Target ORF information:

    RefSeq Version XM_004910697.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neurofascin (nfasc), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004910697.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGACAGATT TTCTAGAAAG GAGGTTAACG GTGATCCACT ACTACTGTCT CGGAAGCAAC 
    ATGCCATCAT GTTTAGTCCT TCTCCTGCTC TTTCTCCACC TAGTCTGCAC TATTAAAGTT
    CCACTGGACC TAATACAGCC CCCAACCATC ATCAAGCAGT CAGCAAAGAA TTATATTGTA
    GATCCCCGTG ATAACATCCT AATTGAGTGT GAAGCTCAGG GGAATCCTTA CCCAATGTTT
    TCATGGACAA AAAATGGGAA ATTTTACAAT GTGGCAAAAG ACCCTCGAGT GTCATTCCGT
    CGACGATCAG GCACCCTTGA AATAGAAATT CATGGAGGAG GGCGGCCAGA GGACTATGAG
    GGAGAATACC AATGCTCTGC CCGCAACCAA CATGGAACAG CTTTATCAAA TAAGATCTTA
    CTTCAAGTCT CTCGATCCCC TTTGTGGCCC AAAGAAAACC TTGACCCGGT GGTGGTAAAT
    GAAGGTGCCT TCTTGATCCT TCAGTGCAAT CCCCCACATG GACTTCCTCC TCCAGTCATA
    TTTTGGATGA GCAGCTTGAT GGAGCCTATT GTCCAGGACA AGAGGGTTTC TCAGGGACTG
    AATGGGGACC TTTATTTCTC CAACGTTCTG CGCCAGGATA CTCAGACTGA TTATAGCTGC
    AATGCACGCT CCCATTTCAC ACACACCATT CAACAGAAGA ACCCATATAC TCTAAAAGTG
    CTTACCACCA GAGGGGTACC TGAGAGAACC CCAACCTTCT TGCTTCCCAC GGGGACTTCT
    AGCAGTAAAC TGGTGTTACG CGATGAGGAC TTGCTACTGG AGTGTATTGC TTCTGGAGTA
    CCAACGCCAG ACATCATGTG GTATAAGAAA GGAGGAGAGC TACCCAGCAG GAAGCTGAAG
    TTTGAAAATT TTAACAAGAC TCTACGCATA ATTGGAGTGT CTGAAGAGGA CTCGGGGGAG
    TATTTCTGCC AAGCTTCAAA CAAAATGGGC AGCATTCGGC ACACGATTTC TGTTCGGGTT
    AAGGCTGCTC CATATTGGCT TGATGAGCCC AAGAACTTGA TTCTTGCACC AGGTGAGAAC
    GGACGTTTGG TATGTCGTGC CAGTGGCAGC CCCAAACCTA CAATCCAATG GTTTATGAAT
    GGAGATGCTA CTGAAGTAGG TGCCCCAAAT CCCAACTTCG AAATCGCTGG TGATACCATT
    ATTTTTAGAG AAGTACAGAG TGGCAACAGC GCTGTATACC AATGCAATGC TTCTAATGAA
    CATGGTTATC TGTTAGCTAA TGCCTTCGTC AGCATTCTGG ATGTTCCATC TCGGATGTTG
    GGCCCTCGTA ATCAGCTGAT TCGAGTGATA GAATACAACA GGACCCAGCT AGACTGCCCT
    TTCTTTGGCT CTCCTATACC TACACTCCGC TGGTTTAAAA ATGGACAGGG TAGTAACCTC
    GATGGTGGCA ATTACCGGGT GCATGAAAAT GGGACCCTTG AGATTAATAC TGTGAGGAAG
    GAGGACCAAG GCAAATACAC ATGTGTTGCT ACCAATATTT TGGGCAATGC TGAAAATACT
    GTGCGGCTGG AGGTCAAAGA GCCAACTCGT ATTGTAAAGG GCCCTGAAGA TCAGATTGCG
    AAGAGGGGCT CTTCTGTGCA TCTGGACTGT CGAGTGCGCA GTGACTCCTC ACTCAGACTC
    ACTGTTACCT GGATAAAAGA TGACACTCAG TTGTATATAA ACAGCAGAAT GAAGAAGGAT
    GAGGATGGAC TTACAATATT CGGTGTGGCA GAAAAGGATC AGGGAGCATA TACATGTGTT
    GCTAAAACTG AATTAGACCA GGACTCTTCT AAAGCTTATC TCACGATACA GGAAACTTCC
    TCACCTACTA GCCGACTGAC CTCCCAGCTT ATAGACAAAC CAGACCCCCC ACAGGACCTG
    GAGCTAACTG AACCATCAGT TAGGAGTGTT CGCCTCACCT GGGTTCCAGG GGATGATAAC
    AACAGTCCAA TTACAGGTTT CATTGTACAA TTTGAGGAAG ACAGGCATCA GCCCGGTACA
    TGGCACAACC TTGCCTCTGT CCCTGCTAAT GTGAACTCTG TCATACTGCA GCTTTCTCCT
    TATGTCAACT ACCAGTTTCG TGTTCTTGCA CTAAATGAAC TTGGGAGCAG TATGCCAAGC
    CCATCTTCAG AACGTTTTCA GACAGCAGGA GCACCTCCAG AAACTAATCC AGGTGATGTG
    AAGGGAGCAG GGACTAGCAA AAACAATATG GAAATCACAT GGACACCACT GAACTCCACT
    CAGGCGTATG GTCCAAACCT GCGCTACAAC GTGAAGTGGC GCCAACGTCA CACAGGAGAA
    CCTTGGAGCA ATATTACAGT GTGGGACAAC AAGCATGTGG TGTGGAATAC ACCAGTATAC
    ACCCAATATG AGCTAAGAGT TCAGGCGGAA AATGAATTTG GAAAGGCTCC AGAGCCAAGC
    ACCATGATTG GATATTCTGG TGAAGACTAT CCTAAGGCTG CACCCACTGA CATTAAGCTT
    CGGGTCATGA ACAGCACCGC CATCTCTCTT CAGTGGACAC GTGTGGCACC TGCCTCTATC
    CAAGGAAGCC TACTGGAATA CAGAGCTTAT TTTTGGAGAG AAAGTAGTTT GCTGAAGGGT
    GTCTGGGTCT CACGAAAGAA GCAACAACAG AGTTTTATGG GGGACCGTGT GAGAGGCATC
    GTATCTCAAC TGATTCCATA CAGCATCTAC AAGCTGTACT TGGTGGCAGT GAATGGCCGA
    GGGGATGGCC CCAAAAGTGA TGTTAAGGAA TTCACAACCC CAGAGGGAGT GCCGAGTGCT
    CCTAAATACT TTAGAGTATA TGAGCCAAAC CGGGAAATGA TTGTTGTTGA ATGGGGTCAC
    CCAGAGCACC CGAACGGAAA CTTGACTGCA TACAACCTCC GCATCCAACC ATTTAATGGC
    ACCAGAACAG GGAAGGCTGT ATTTGAAACT CTTTCACGTA ATCAAACCAA ATATACCCTG
    CAAAGAACAG ATCCCGTGTC CCGGTATCGG TTTGCTATCA ATGCCAGAAC GCAGGTTGGT
    GCAGGGGAAG TCATCACAGC TGAGTCTCCT GTCAGACTGG ATGAAGTTTC TCCCACGTCA
    GTGTCTTCTA AGTCTCCTGC ACCTGTCACA GATGCTCCCA CAGAGGTCCC AACTATTCCC
    CCAACCTCAT CTGGTGCCAC CACTCTTCTA GCTACAACCA GTACAACCAG TGTCACAACG
    ACTACTGCTG GTACAGAAAG TTCGCTGGTC GCCATTACTA GAATTCCTAA TATAGCCACC
    ACTGGACCAT CTCTATGGAA CCTAACAGCA TTACCCAACA CTTACTGGGC AAACATCACC
    TGGATGCATA ACTTAGAGCC AGGCTCTGTG TTTATGGTTG AGTATACTGA CAGTAATAAT
    ACAGTGAAAA CTGTTCCTGT TAAGGCTCAT GTCCCTTCTA TCCAACTGTT TGATCTGGTG
    CCTGGGATGA CCTACAGGGT GCGGGTTTAC TCCCAGGAGC ACCCGTCAGT CACAAGCCTA
    TATGTGACAT TTGATACCAG CTCAGCTTAC ACCAAGAACC AAGTTGACAT TGCTACACAA
    GGCTGGTTTA TTGGGCTCAT GTGTGCAGTT GCTCTACTGG TACTGATTTT GCTGATCGTT
    TGTTTTATCA AAAGAAGCCG AGGAGGCAAA TACCCAGTGC GAGAAAAGAA GGAGGTGGCG
    TTGGGACCAG ATGATAACAA AGAGGGGGAT GGCTCATTTG ACTACAGTGA TGAGGACAAC
    AAGCCCCTGC AGGGTAGTCA AACTTCGCTG GATGGAACCA TAAAGCAACA AGAGAGTGAT
    GACAGCCTTG TTGATTATGG AGAAGGAGGA GAGGGGCAGT TTAATGAAGA TGGATCGTTT
    ATAGGGCAGT ATACAGTGAA AAAGGACAAA GATGAGACTG AGGGAAATGA AAGTTCAGAG
    GCCACATCTC CTGTAAATGC TATCTATTCT CTGGCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa03812
    Clone ID Related Accession (Same CDS sequence) XM_031896834.1
    Accession Version XM_031896834.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3696bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neurofascin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGACAGATT TTCTAGAAAG GAGGTTAACG GTGATCCACT ACTACTGTCT CGGAAGCAAC 
    ATGCCATCAT GTTTAGTCCT TCTCCTGCTC TTTCTCCACC TAGTCTGCAC TATTAAAGTT
    CCACTGGACC CAAACATTCA GCGAGACCTA ATACAGCCCC CAACCATCAT CAAGCAGTCA
    GCAAAGAATT ATATTGTAGA TCCCCGTGAT AACATCCTAA TTGAGTGTGA AGCTCAGGGG
    AATCCTTACC CAATGTTTTC ATGGACAAAA AATGGGAAAT TTTACAATGT GGCAAAAGAC
    CCTCGAGTGT CATTCCGTCG ACGATCAGGC ACCCTTGAAA TAGAAATTCA TGGAGGAGGG
    CGGCCAGAGG ACTATGAGGG AGAATACCAA TGCTCTGCCC GCAACCAACA TGGAACAGCT
    TTATCAAATA AGATCTTACT TCAAGTCTCT CGATCCCCTT TGTGGCCCAA AGAAAACCTT
    GACCCGGTGG TGGTAAATGA AGGTGCCTTC TTGATCCTTC AGTGCAATCC CCCACATGGA
    CTTCCTCCTC CAGTCATATT TTGGATGAGC AGCTTGATGG AGCCTATTGT CCAGGACAAG
    AGGGTTTCTC AGGGACTGAA TGGGGACCTT TATTTCTCCA ACGTTCTGCG CCAGGATACT
    CAGACTGATT ATAGCTGCAA TGCACGCTCC CATTTCACAC ACACCATTCA ACAGAAGAAC
    CCATATACTC TAAAAGTGCT TACCACCAGA GGGGTACCTG AGAGAACCCC AACCTTCTTG
    CTTCCCACGG GGACTTCTAG CAGTAAACTG GTGTTACGCG ATGAGGACTT GCTACTGGAG
    TGTATTGCTT CTGGAGTACC AACGCCAGAC ATCATGTGGT ATAAGAAAGG AGGAGAGCTA
    CCCAGCAGGA AGCTGAAGTT TGAAAATTTT AACAAGACTC TACGCATAAT TGGAGTGTCT
    GAAGAGGACT CGGGGGAGTA TTTCTGCCAA GCTTCAAACA AAATGGGCAG CATTCGGCAC
    ACGATTTCTG TTCGGGTTAA GGCTGCTCCA TATTGGCTTG ATGAGCCCAA GAACTTGATT
    CTTGCACCAG GTGAGAACGG ACGTTTGGTA TGTCGTGCCA GTGGCAGCCC CAAACCTACA
    ATCCAATGGT TTATGAATGG AGATGCTACT GAAGTAGGTG CCCCAAATCC CAACTTCGAA
    ATCGCTGGTG ATACCATTAT TTTTAGAGAA GTACAGAGTG GCAACAGCGC TGTATACCAA
    TGCAATGCTT CTAATGAACA TGGTTATCTG TTAGCTAATG CCTTCGTCAG CATTCTGGAT
    GTTCCATCTC GGATGTTGGG CCCTCGTAAT CAGCTGATTC GAGTGATAGA ATACAACAGG
    ACCCAGCTAG ACTGCCCTTT CTTTGGCTCT CCTATACCTA CACTCCGCTG GTTTAAAAAT
    GGACAGGGTA GTAACCTCGA TGGTGGCAAT TACCGGGTGC ATGAAAATGG GACCCTTGAG
    ATTAATACTG TGAGGAAGGA GGACCAAGGC AAATACACAT GTGTTGCTAC CAATATTTTG
    GGCAATGCTG AAAATACTGT GCGGCTGGAG GTCAAAGAGC CAACTCGTAT TGTAAAGGGC
    CCTGAAGATC AGATTGCGAA GAGGGGCTCT TCTGTGCATC TGGACTGTCG AGTGCGCAGT
    GACTCCTCAC TCAGACTCAC TGTTACCTGG ATAAAAGATG ACACTCAGTT GTATATAAAC
    AGCAGAATGA AGAAGGATGA GGATGGACTT ACAATATTCG GTGTGGCAGA AAAGGATCAG
    GGAGCATATA CATGTGTTGC TAAAACTGAA TTAGACCAGG ACTCTTCTAA AGCTTATCTC
    ACGATACAGG AAACTTCCTC ACCTACTAGC CGACTGACCT CCCAGCTTAT AGACAAACCA
    GACCCCCCAC AGGACCTGGA GCTAACTGAA CCATCAGTTA GGAGTGTTCG CCTCACCTGG
    GTTCCAGGGG ATGATAACAA CAGTCCAATT ACAGGTTTCA TTGTACAATT TGAGGAAGAC
    AGGCATCAGC CCGGTACATG GCACAACCTT GCCTCTGTCC CTGCTAATGT GAACTCTGTC
    ATACTGCAGC TTTCTCCTTA TGTCAACTAC CAGTTTCGTG TTCTTGCACT AAATGAACTT
    GGGAGCAGTA TGCCAAGCCC ATCTTCAGAA CGTTTTCAGA CAGCAGGAGC ACCTCCAGAA
    ACTAATCCAG GTGATGTGAA GGGAGCAGGG ACTAGCAAAA ACAATATGGA AATCACATGG
    ACACCACTGA ACTCCACTCA GGCGTATGGT CCAAACCTGC GCTACAACGT GAAGTGGCGC
    CAACGTCACA CAGGAGAACC TTGGAGCAAT ATTACAGTGT GGGACAACAA GCATGTGGTG
    TGGAATACAC CAGTATACAC CCAATATGAG CTAAGAGTTC AGGCGGAAAA TGAATTTGGA
    AAGGCTCCAG AGCCAAGCAC CATGATTGGA TATTCTGGTG AAGACTTGCC GAGTGCTCCT
    AAATACTTTA GAGTATATGA GCCAAACCGG GAAATGATTG TTGTTGAATG GGGTCACCCA
    GAGCACCCGA ACGGAAACTT GACTGCATAC AACCTCCGCA TCCAACCATT TAATGGCACC
    AGAACAGGGA AGGCTGTATT TGAAACTCTT TCACGTAATC AAACCAAATA TACCCTGCAA
    AGAACAGATC CCGTGTCCCG GTATCGGTTT GCTATCAATG CCAGAACGCA GGTTGGTGCA
    GGGGAAGTCA TCACAGCTGA GTCTCCTGTC AGACTGGATG AAGTTTCTCC CACGTCAGTG
    TCTTCTAAGT CTCCTGCACC TGTCACAGAT GCTCCCACAG AGGTCCCAAC TATTCCCCCA
    ACCTCATCTG GTGCCACCAC TCTTCTAGCT ACAACCAGTA CAACCAGTGT CACAACGACT
    ACTGCTGGTA CAGAAAGTTC GCTGGTCGCC ATTACTAGAA TTCCTAATAT AGCCACCACT
    GGACCATCTC TATGGAACCT AACAGCATTA CCCAACACTT ACTGGGCAAA CATCACCTGG
    ATGCATAACT TAGAGCCAGG CTCTGTGTTT ATGGTTGAGT ATACTGACAG TAATAATACA
    GTGAAAACTG TTCCTGTTAA GGCTCATGTC CCTTCTATCC AACTGTTTGA TCTGGTGCCT
    GGGATGACCT ACAGGGTGCG GGTTTACTCC CAGGAGCACC CGTCAGTCAC AAGCCTATAT
    GTGACATTTG ATACCAGCTC AGCTTACACC AAGAACCAAG TTGACATTGC TACACAAGGC
    TGGTTTATTG GGCTCATGTG TGCAGTTGCT CTACTGGTAC TGATTTTGCT GATCGTTTGT
    TTTATCAAAA GAAGCCGAGG AGGCAAATAC CCAGTGCGAG AAAAGAAGGA GGTGGCGTTG
    GGACCAGATG ATAACAAAGA GGGGGATGGC TCATTTGACT ACAGTGATGA GGACAACAAG
    CCCCTGCAGG GTAGTCAAAC TTCGCTGGAT GGAACCATAA AGCAACAAGA GAGTGATGAC
    AGCCTTGTTG ATTATGGAGA AGGAGGAGAG GGGCAGTTTA ATGAAGATGG ATCGTTTATA
    GGGCAGTATA CAGTGAAAAA GGACAAAGAT GAGACTGAGG GAAATGAAAG TTCAGAGGCC
    ACATCTCCTG TAAATGCTAT CTATTCTCTG GCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031752694.1
    CDS545..4240
    Translation

    Target ORF information:

    RefSeq Version XM_031896834.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neurofascin (nfasc), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896834.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGACAGATT TTCTAGAAAG GAGGTTAACG GTGATCCACT ACTACTGTCT CGGAAGCAAC 
    ATGCCATCAT GTTTAGTCCT TCTCCTGCTC TTTCTCCACC TAGTCTGCAC TATTAAAGTT
    CCACTGGACC CAAACATTCA GCGAGACCTA ATACAGCCCC CAACCATCAT CAAGCAGTCA
    GCAAAGAATT ATATTGTAGA TCCCCGTGAT AACATCCTAA TTGAGTGTGA AGCTCAGGGG
    AATCCTTACC CAATGTTTTC ATGGACAAAA AATGGGAAAT TTTACAATGT GGCAAAAGAC
    CCTCGAGTGT CATTCCGTCG ACGATCAGGC ACCCTTGAAA TAGAAATTCA TGGAGGAGGG
    CGGCCAGAGG ACTATGAGGG AGAATACCAA TGCTCTGCCC GCAACCAACA TGGAACAGCT
    TTATCAAATA AGATCTTACT TCAAGTCTCT CGATCCCCTT TGTGGCCCAA AGAAAACCTT
    GACCCGGTGG TGGTAAATGA AGGTGCCTTC TTGATCCTTC AGTGCAATCC CCCACATGGA
    CTTCCTCCTC CAGTCATATT TTGGATGAGC AGCTTGATGG AGCCTATTGT CCAGGACAAG
    AGGGTTTCTC AGGGACTGAA TGGGGACCTT TATTTCTCCA ACGTTCTGCG CCAGGATACT
    CAGACTGATT ATAGCTGCAA TGCACGCTCC CATTTCACAC ACACCATTCA ACAGAAGAAC
    CCATATACTC TAAAAGTGCT TACCACCAGA GGGGTACCTG AGAGAACCCC AACCTTCTTG
    CTTCCCACGG GGACTTCTAG CAGTAAACTG GTGTTACGCG ATGAGGACTT GCTACTGGAG
    TGTATTGCTT CTGGAGTACC AACGCCAGAC ATCATGTGGT ATAAGAAAGG AGGAGAGCTA
    CCCAGCAGGA AGCTGAAGTT TGAAAATTTT AACAAGACTC TACGCATAAT TGGAGTGTCT
    GAAGAGGACT CGGGGGAGTA TTTCTGCCAA GCTTCAAACA AAATGGGCAG CATTCGGCAC
    ACGATTTCTG TTCGGGTTAA GGCTGCTCCA TATTGGCTTG ATGAGCCCAA GAACTTGATT
    CTTGCACCAG GTGAGAACGG ACGTTTGGTA TGTCGTGCCA GTGGCAGCCC CAAACCTACA
    ATCCAATGGT TTATGAATGG AGATGCTACT GAAGTAGGTG CCCCAAATCC CAACTTCGAA
    ATCGCTGGTG ATACCATTAT TTTTAGAGAA GTACAGAGTG GCAACAGCGC TGTATACCAA
    TGCAATGCTT CTAATGAACA TGGTTATCTG TTAGCTAATG CCTTCGTCAG CATTCTGGAT
    GTTCCATCTC GGATGTTGGG CCCTCGTAAT CAGCTGATTC GAGTGATAGA ATACAACAGG
    ACCCAGCTAG ACTGCCCTTT CTTTGGCTCT CCTATACCTA CACTCCGCTG GTTTAAAAAT
    GGACAGGGTA GTAACCTCGA TGGTGGCAAT TACCGGGTGC ATGAAAATGG GACCCTTGAG
    ATTAATACTG TGAGGAAGGA GGACCAAGGC AAATACACAT GTGTTGCTAC CAATATTTTG
    GGCAATGCTG AAAATACTGT GCGGCTGGAG GTCAAAGAGC CAACTCGTAT TGTAAAGGGC
    CCTGAAGATC AGATTGCGAA GAGGGGCTCT TCTGTGCATC TGGACTGTCG AGTGCGCAGT
    GACTCCTCAC TCAGACTCAC TGTTACCTGG ATAAAAGATG ACACTCAGTT GTATATAAAC
    AGCAGAATGA AGAAGGATGA GGATGGACTT ACAATATTCG GTGTGGCAGA AAAGGATCAG
    GGAGCATATA CATGTGTTGC TAAAACTGAA TTAGACCAGG ACTCTTCTAA AGCTTATCTC
    ACGATACAGG AAACTTCCTC ACCTACTAGC CGACTGACCT CCCAGCTTAT AGACAAACCA
    GACCCCCCAC AGGACCTGGA GCTAACTGAA CCATCAGTTA GGAGTGTTCG CCTCACCTGG
    GTTCCAGGGG ATGATAACAA CAGTCCAATT ACAGGTTTCA TTGTACAATT TGAGGAAGAC
    AGGCATCAGC CCGGTACATG GCACAACCTT GCCTCTGTCC CTGCTAATGT GAACTCTGTC
    ATACTGCAGC TTTCTCCTTA TGTCAACTAC CAGTTTCGTG TTCTTGCACT AAATGAACTT
    GGGAGCAGTA TGCCAAGCCC ATCTTCAGAA CGTTTTCAGA CAGCAGGAGC ACCTCCAGAA
    ACTAATCCAG GTGATGTGAA GGGAGCAGGG ACTAGCAAAA ACAATATGGA AATCACATGG
    ACACCACTGA ACTCCACTCA GGCGTATGGT CCAAACCTGC GCTACAACGT GAAGTGGCGC
    CAACGTCACA CAGGAGAACC TTGGAGCAAT ATTACAGTGT GGGACAACAA GCATGTGGTG
    TGGAATACAC CAGTATACAC CCAATATGAG CTAAGAGTTC AGGCGGAAAA TGAATTTGGA
    AAGGCTCCAG AGCCAAGCAC CATGATTGGA TATTCTGGTG AAGACTTGCC GAGTGCTCCT
    AAATACTTTA GAGTATATGA GCCAAACCGG GAAATGATTG TTGTTGAATG GGGTCACCCA
    GAGCACCCGA ACGGAAACTT GACTGCATAC AACCTCCGCA TCCAACCATT TAATGGCACC
    AGAACAGGGA AGGCTGTATT TGAAACTCTT TCACGTAATC AAACCAAATA TACCCTGCAA
    AGAACAGATC CCGTGTCCCG GTATCGGTTT GCTATCAATG CCAGAACGCA GGTTGGTGCA
    GGGGAAGTCA TCACAGCTGA GTCTCCTGTC AGACTGGATG AAGTTTCTCC CACGTCAGTG
    TCTTCTAAGT CTCCTGCACC TGTCACAGAT GCTCCCACAG AGGTCCCAAC TATTCCCCCA
    ACCTCATCTG GTGCCACCAC TCTTCTAGCT ACAACCAGTA CAACCAGTGT CACAACGACT
    ACTGCTGGTA CAGAAAGTTC GCTGGTCGCC ATTACTAGAA TTCCTAATAT AGCCACCACT
    GGACCATCTC TATGGAACCT AACAGCATTA CCCAACACTT ACTGGGCAAA CATCACCTGG
    ATGCATAACT TAGAGCCAGG CTCTGTGTTT ATGGTTGAGT ATACTGACAG TAATAATACA
    GTGAAAACTG TTCCTGTTAA GGCTCATGTC CCTTCTATCC AACTGTTTGA TCTGGTGCCT
    GGGATGACCT ACAGGGTGCG GGTTTACTCC CAGGAGCACC CGTCAGTCAC AAGCCTATAT
    GTGACATTTG ATACCAGCTC AGCTTACACC AAGAACCAAG TTGACATTGC TACACAAGGC
    TGGTTTATTG GGCTCATGTG TGCAGTTGCT CTACTGGTAC TGATTTTGCT GATCGTTTGT
    TTTATCAAAA GAAGCCGAGG AGGCAAATAC CCAGTGCGAG AAAAGAAGGA GGTGGCGTTG
    GGACCAGATG ATAACAAAGA GGGGGATGGC TCATTTGACT ACAGTGATGA GGACAACAAG
    CCCCTGCAGG GTAGTCAAAC TTCGCTGGAT GGAACCATAA AGCAACAAGA GAGTGATGAC
    AGCCTTGTTG ATTATGGAGA AGGAGGAGAG GGGCAGTTTA ATGAAGATGG ATCGTTTATA
    GGGCAGTATA CAGTGAAAAA GGACAAAGAT GAGACTGAGG GAAATGAAAG TTCAGAGGCC
    ACATCTCCTG TAAATGCTAT CTATTCTCTG GCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa03813
    Clone ID Related Accession (Same CDS sequence) XM_031896836.1
    Accession Version XM_031896836.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3558bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neurofascin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGACAGATT TTCTAGAAAG GAGGTTAACG GTGATCCACT ACTACTGTCT CGGAAGCAAC 
    ATGCCATCAT GTTTAGTCCT TCTCCTGCTC TTTCTCCACC TAGTCTGCAC TATTAAAGTT
    CCACTGGACC CAAACATTCA GCGAGACCTA ATACAGCCCC CAACCATCAT CAAGCAGTCA
    GCAAAGAATT ATATTGTAGA TCCCCGTGAT AACATCCTAA TTGAGTGTGA AGCTCAGGGG
    AATCCTTACC CAATGTTTTC ATGGACAAAA AATGGGAAAT TTTACAATGT GGCAAAAGAC
    CCTCGAGTGT CATTCCGTCG ACGATCAGGC ACCCTTGAAA TAGAAATTCA TGGAGGAGGG
    CGGCCAGAGG ACTATGAGGG AGAATACCAA TGCTCTGCCC GCAACCAACA TGGAACAGCT
    TTATCAAATA AGATCTTACT TCAAGTCTCT CGATCCCCTT TGTGGCCCAA AGAAAACCTT
    GACCCGGTGG TGGTAAATGA AGGTGCCTTC TTGATCCTTC AGTGCAATCC CCCACATGGA
    CTTCCTCCTC CAGTCATATT TTGGATGAGC AGCTTGATGG AGCCTATTGT CCAGGACAAG
    AGGGTTTCTC AGGGACTGAA TGGGGACCTT TATTTCTCCA ACGTTCTGCG CCAGGATACT
    CAGACTGATT ATAGCTGCAA TGCACGCTCC CATTTCACAC ACACCATTCA ACAGAAGAAC
    CCATATACTC TAAAAGTGCT TACCACCAGA GGGGTACCTG AGAGAACCCC AACCTTCTTG
    CTTCCCACGG GGACTTCTAG CAGTAAACTG GTGTTACGCG ATGAGGACTT GCTACTGGAG
    TGTATTGCTT CTGGAGTACC AACGCCAGAC ATCATGTGGT ATAAGAAAGG AGGAGAGCTA
    CCCAGCAGGA AGCTGAAGTT TGAAAATTTT AACAAGACTC TACGCATAAT TGGAGTGTCT
    GAAGAGGACT CGGGGGAGTA TTTCTGCCAA GCTTCAAACA AAATGGGCAG CATTCGGCAC
    ACGATTTCTG TTCGGGTTAA GGCTGCTCCA TATTGGCTTG ATGAGCCCAA GAACTTGATT
    CTTGCACCAG GTGAGAACGG ACGTTTGGTA TGTCGTGCCA GTGGCAGCCC CAAACCTACA
    ATCCAATGGT TTATGAATGG AGATGCTACT GAAGTAGGTG CCCCAAATCC CAACTTCGAA
    ATCGCTGGTG ATACCATTAT TTTTAGAGAA GTACAGAGTG GCAACAGCGC TGTATACCAA
    TGCAATGCTT CTAATGAACA TGGTTATCTG TTAGCTAATG CCTTCGTCAG CATTCTGGAT
    GTTCCATCTC GGATGTTGGG CCCTCGTAAT CAGCTGATTC GAGTGATAGA ATACAACAGG
    ACCCAGCTAG ACTGCCCTTT CTTTGGCTCT CCTATACCTA CACTCCGCTG GTTTAAAAAT
    GGACAGGGTA GTAACCTCGA TGGTGGCAAT TACCGGGTGC ATGAAAATGG GACCCTTGAG
    ATTAATACTG TGAGGAAGGA GGACCAAGGC AAATACACAT GTGTTGCTAC CAATATTTTG
    GGCAATGCTG AAAATACTGT GCGGCTGGAG GTCAAAGAGC CAACTCGTAT TGTAAAGGGC
    CCTGAAGATC AGATTGCGAA GAGGGGCTCT TCTGTGCATC TGGACTGTCG AGTGCGCAGT
    GACTCCTCAC TCAGACTCAC TGTTACCTGG ATAAAAGATG ACACTCAGTT GTATATAAAC
    AGCAGAATGA AGAAGGATGA GGATGGACTT ACAATATTCG GTGTGGCAGA AAAGGATCAG
    GGAGCATATA CATGTGTTGC TAAAACTGAA TTAGACCAGG ACTCTTCTAA AGCTTATCTC
    ACGATACAGG AAACTTCCTC ACCTACTAGC CGACTGACCT CCCAGCTTAT AGACAAACCA
    GACCCCCCAC AGGACCTGGA GCTAACTGAA CCATCAGTTA GGAGTGTTCG CCTCACCTGG
    GTTCCAGGGG ATGATAACAA CAGTCCAATT ACAGGTTTCA TTGTACAATT TGAGGAAGAC
    AGGCATCAGC CCGGTACATG GCACAACCTT GCCTCTGTCC CTGCTAATGT GAACTCTGTC
    ATACTGCAGC TTTCTCCTTA TGTCAACTAC CAGTTTCGTG TTCTTGCACT AAATGAACTT
    GGGAGCAGTA TGCCAAGCCC ATCTTCAGAA CGTTTTCAGA CAGCAGGAGC ACCTCCAGAA
    ACTAATCCAG GTGATGTGAA GGGAGCAGGG ACTAGCAAAA ACAATATGGA AATCACATGG
    ACACCACTGA ACTCCACTCA GGCGTATGGT CCAAACCTGC GCTACAACGT GAAGTGGCGC
    CAACGTCACA CAGGAGAACC TTGGAGCAAT ATTACAGTGT GGGACAACAA GCATGTGGTG
    TGGAATACAC CAGTATACAC CCAATATGAG CTAAGAGTTC AGGCGGAAAA TGAATTTGGA
    AAGGCTCCAG AGCCAAGCAC CATGATTGGA TATTCTGGTG AAGACTATCC TAAGGCTGCA
    CCCACTGACA TTAAGCTTCG GGTCATGAAC AGCACCGCCA TCTCTCTTCA GTGGACACGT
    GTGGCACCTG CCTCTATCCA AGGAAGCCTA CTGGAATACA GAGCTTATTT TTGGAGAGAA
    AGTAGTTTGC TGAAGGGTGT CTGGGTCTCA CGAAAGAAGC AACAACAGAG TTTTATGGGG
    GACCGTGTGA GAGGCATCGT ATCTCAACTG ATTCCATACA GCATCTACAA GCTGTACTTG
    GTGGCAGTGA ATGGCCGAGG GGATGGCCCC AAAAGTGATG TTAAGGAATT CACAACCCCA
    GAGGGAGTGC CGAGTGCTCC TAAATACTTT AGAGTATATG AGCCAAACCG GGAAATGATT
    GTTGTTGAAT GGGGTCACCC AGAGCACCCG AACGGAAACT TGACTGCATA CAACCTCCGC
    ATCCAACCAT TTAATGGCAC CAGAACAGGG AAGGCTGTAT TTGAAACTCT TTCACGTAAT
    CAAACCAAAT ATACCCTGCA AAGAACAGAT CCCGTGTCCC GGTATCGGTT TGCTATCAAT
    GCCAGAACGC AGGTTGGTGC AGGGGAAGTC ATCACAGCTG AGTCTCCTGT CAGACTGGAT
    GAAGCTTACA CCAAGAACCA AGTTGACATT GCTACACAAG GCTGGTTTAT TGGGCTCATG
    TGTGCAGTTG CTCTACTGGT ACTGATTTTG CTGATCGTTT GTTTTATCAA AAGAAGCCGA
    GGAGGCAAAT ACCCAGTGCG AGAAAAGAAG GAGGTGGCGT TGGGACCAGA TGATAACAAA
    GAGGGGGATG GCTCATTTGA CTACAGTGAT GAGGACAACA AGCCCCTGCA GGGTAGTCAA
    ACTTCGCTGG ATGGAACCAT AAAGCAACAA GAGAGTGATG ACAGCCTTGT TGATTATGGA
    GAAGGAGGAG AGGGGCAGTT TAATGAAGAT GGATCGTTTA TAGGGCAGTA TACAGTGAAA
    AAGGACAAAG ATGAGACTGA GGGAAATGAA AGTTCAGAGG CCACATCTCC TGTAAATGCT
    ATCTATTCTC TGGCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031752696.1
    CDS545..4102
    Translation

    Target ORF information:

    RefSeq Version XM_031896836.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neurofascin (nfasc), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896836.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGACAGATT TTCTAGAAAG GAGGTTAACG GTGATCCACT ACTACTGTCT CGGAAGCAAC 
    ATGCCATCAT GTTTAGTCCT TCTCCTGCTC TTTCTCCACC TAGTCTGCAC TATTAAAGTT
    CCACTGGACC CAAACATTCA GCGAGACCTA ATACAGCCCC CAACCATCAT CAAGCAGTCA
    GCAAAGAATT ATATTGTAGA TCCCCGTGAT AACATCCTAA TTGAGTGTGA AGCTCAGGGG
    AATCCTTACC CAATGTTTTC ATGGACAAAA AATGGGAAAT TTTACAATGT GGCAAAAGAC
    CCTCGAGTGT CATTCCGTCG ACGATCAGGC ACCCTTGAAA TAGAAATTCA TGGAGGAGGG
    CGGCCAGAGG ACTATGAGGG AGAATACCAA TGCTCTGCCC GCAACCAACA TGGAACAGCT
    TTATCAAATA AGATCTTACT TCAAGTCTCT CGATCCCCTT TGTGGCCCAA AGAAAACCTT
    GACCCGGTGG TGGTAAATGA AGGTGCCTTC TTGATCCTTC AGTGCAATCC CCCACATGGA
    CTTCCTCCTC CAGTCATATT TTGGATGAGC AGCTTGATGG AGCCTATTGT CCAGGACAAG
    AGGGTTTCTC AGGGACTGAA TGGGGACCTT TATTTCTCCA ACGTTCTGCG CCAGGATACT
    CAGACTGATT ATAGCTGCAA TGCACGCTCC CATTTCACAC ACACCATTCA ACAGAAGAAC
    CCATATACTC TAAAAGTGCT TACCACCAGA GGGGTACCTG AGAGAACCCC AACCTTCTTG
    CTTCCCACGG GGACTTCTAG CAGTAAACTG GTGTTACGCG ATGAGGACTT GCTACTGGAG
    TGTATTGCTT CTGGAGTACC AACGCCAGAC ATCATGTGGT ATAAGAAAGG AGGAGAGCTA
    CCCAGCAGGA AGCTGAAGTT TGAAAATTTT AACAAGACTC TACGCATAAT TGGAGTGTCT
    GAAGAGGACT CGGGGGAGTA TTTCTGCCAA GCTTCAAACA AAATGGGCAG CATTCGGCAC
    ACGATTTCTG TTCGGGTTAA GGCTGCTCCA TATTGGCTTG ATGAGCCCAA GAACTTGATT
    CTTGCACCAG GTGAGAACGG ACGTTTGGTA TGTCGTGCCA GTGGCAGCCC CAAACCTACA
    ATCCAATGGT TTATGAATGG AGATGCTACT GAAGTAGGTG CCCCAAATCC CAACTTCGAA
    ATCGCTGGTG ATACCATTAT TTTTAGAGAA GTACAGAGTG GCAACAGCGC TGTATACCAA
    TGCAATGCTT CTAATGAACA TGGTTATCTG TTAGCTAATG CCTTCGTCAG CATTCTGGAT
    GTTCCATCTC GGATGTTGGG CCCTCGTAAT CAGCTGATTC GAGTGATAGA ATACAACAGG
    ACCCAGCTAG ACTGCCCTTT CTTTGGCTCT CCTATACCTA CACTCCGCTG GTTTAAAAAT
    GGACAGGGTA GTAACCTCGA TGGTGGCAAT TACCGGGTGC ATGAAAATGG GACCCTTGAG
    ATTAATACTG TGAGGAAGGA GGACCAAGGC AAATACACAT GTGTTGCTAC CAATATTTTG
    GGCAATGCTG AAAATACTGT GCGGCTGGAG GTCAAAGAGC CAACTCGTAT TGTAAAGGGC
    CCTGAAGATC AGATTGCGAA GAGGGGCTCT TCTGTGCATC TGGACTGTCG AGTGCGCAGT
    GACTCCTCAC TCAGACTCAC TGTTACCTGG ATAAAAGATG ACACTCAGTT GTATATAAAC
    AGCAGAATGA AGAAGGATGA GGATGGACTT ACAATATTCG GTGTGGCAGA AAAGGATCAG
    GGAGCATATA CATGTGTTGC TAAAACTGAA TTAGACCAGG ACTCTTCTAA AGCTTATCTC
    ACGATACAGG AAACTTCCTC ACCTACTAGC CGACTGACCT CCCAGCTTAT AGACAAACCA
    GACCCCCCAC AGGACCTGGA GCTAACTGAA CCATCAGTTA GGAGTGTTCG CCTCACCTGG
    GTTCCAGGGG ATGATAACAA CAGTCCAATT ACAGGTTTCA TTGTACAATT TGAGGAAGAC
    AGGCATCAGC CCGGTACATG GCACAACCTT GCCTCTGTCC CTGCTAATGT GAACTCTGTC
    ATACTGCAGC TTTCTCCTTA TGTCAACTAC CAGTTTCGTG TTCTTGCACT AAATGAACTT
    GGGAGCAGTA TGCCAAGCCC ATCTTCAGAA CGTTTTCAGA CAGCAGGAGC ACCTCCAGAA
    ACTAATCCAG GTGATGTGAA GGGAGCAGGG ACTAGCAAAA ACAATATGGA AATCACATGG
    ACACCACTGA ACTCCACTCA GGCGTATGGT CCAAACCTGC GCTACAACGT GAAGTGGCGC
    CAACGTCACA CAGGAGAACC TTGGAGCAAT ATTACAGTGT GGGACAACAA GCATGTGGTG
    TGGAATACAC CAGTATACAC CCAATATGAG CTAAGAGTTC AGGCGGAAAA TGAATTTGGA
    AAGGCTCCAG AGCCAAGCAC CATGATTGGA TATTCTGGTG AAGACTATCC TAAGGCTGCA
    CCCACTGACA TTAAGCTTCG GGTCATGAAC AGCACCGCCA TCTCTCTTCA GTGGACACGT
    GTGGCACCTG CCTCTATCCA AGGAAGCCTA CTGGAATACA GAGCTTATTT TTGGAGAGAA
    AGTAGTTTGC TGAAGGGTGT CTGGGTCTCA CGAAAGAAGC AACAACAGAG TTTTATGGGG
    GACCGTGTGA GAGGCATCGT ATCTCAACTG ATTCCATACA GCATCTACAA GCTGTACTTG
    GTGGCAGTGA ATGGCCGAGG GGATGGCCCC AAAAGTGATG TTAAGGAATT CACAACCCCA
    GAGGGAGTGC CGAGTGCTCC TAAATACTTT AGAGTATATG AGCCAAACCG GGAAATGATT
    GTTGTTGAAT GGGGTCACCC AGAGCACCCG AACGGAAACT TGACTGCATA CAACCTCCGC
    ATCCAACCAT TTAATGGCAC CAGAACAGGG AAGGCTGTAT TTGAAACTCT TTCACGTAAT
    CAAACCAAAT ATACCCTGCA AAGAACAGAT CCCGTGTCCC GGTATCGGTT TGCTATCAAT
    GCCAGAACGC AGGTTGGTGC AGGGGAAGTC ATCACAGCTG AGTCTCCTGT CAGACTGGAT
    GAAGCTTACA CCAAGAACCA AGTTGACATT GCTACACAAG GCTGGTTTAT TGGGCTCATG
    TGTGCAGTTG CTCTACTGGT ACTGATTTTG CTGATCGTTT GTTTTATCAA AAGAAGCCGA
    GGAGGCAAAT ACCCAGTGCG AGAAAAGAAG GAGGTGGCGT TGGGACCAGA TGATAACAAA
    GAGGGGGATG GCTCATTTGA CTACAGTGAT GAGGACAACA AGCCCCTGCA GGGTAGTCAA
    ACTTCGCTGG ATGGAACCAT AAAGCAACAA GAGAGTGATG ACAGCCTTGT TGATTATGGA
    GAAGGAGGAG AGGGGCAGTT TAATGAAGAT GGATCGTTTA TAGGGCAGTA TACAGTGAAA
    AAGGACAAAG ATGAGACTGA GGGAAATGAA AGTTCAGAGG CCACATCTCC TGTAAATGCT
    ATCTATTCTC TGGCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa03814
    Clone ID Related Accession (Same CDS sequence) XM_031896839.1
    Accession Version XM_031896839.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3459bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neurofascin isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGACAGATT TTCTAGAAAG GAGGTTAACG GTGATCCACT ACTACTGTCT CGGAAGCAAC 
    ATGCCATCAT GTTTAGTCCT TCTCCTGCTC TTTCTCCACC TAGTCTGCAC TATTAAAGTT
    CCACTGGACC CAAACATTCA GCGAGACCTA ATACAGCCCC CAACCATCAT CAAGCAGTCA
    GCAAAGAATT ATATTGTAGA TCCCCGTGAT AACATCCTAA TTGAGTGTGA AGCTCAGGGG
    AATCCTTACC CAATGTTTTC ATGGACAAAA AATGGGAAAT TTTACAATGT GGCAAAAGAC
    CCTCGAGTGT CATTCCGTCG ACGATCAGGC ACCCTTGAAA TAGAAATTCA TGGAGGAGGG
    CGGCCAGAGG ACTATGAGGG AGAATACCAA TGCTCTGCCC GCAACCAACA TGGAACAGCT
    TTATCAAATA AGATCTTACT TCAAGTCTCT CGATCCCCTT TGTGGCCCAA AGAAAACCTT
    GACCCGGTGG TGGTAAATGA AGGTGCCTTC TTGATCCTTC AGTGCAATCC CCCACATGGA
    CTTCCTCCTC CAGTCATATT TTGGATGAGC AGCTTGATGG AGCCTATTGT CCAGGACAAG
    AGGGTTTCTC AGGGACTGAA TGGGGACCTT TATTTCTCCA ACGTTCTGCG CCAGGATACT
    CAGACTGATT ATAGCTGCAA TGCACGCTCC CATTTCACAC ACACCATTCA ACAGAAGAAC
    CCATATACTC TAAAAGTGCT TACCACCAGA GGGGTACCTG AGAGAACCCC AACCTTCTTG
    CTTCCCACGG GGACTTCTAG CAGTAAACTG GTGTTACGCG ATGAGGACTT GCTACTGGAG
    TGTATTGCTT CTGGAGTACC AACGCCAGAC ATCATGTGGT ATAAGAAAGG AGGAGAGCTA
    CCCAGCAGGA AGCTGAAGTT TGAAAATTTT AACAAGACTC TACGCATAAT TGGAGTGTCT
    GAAGAGGACT CGGGGGAGTA TTTCTGCCAA GCTTCAAACA AAATGGGCAG CATTCGGCAC
    ACGATTTCTG TTCGGGTTAA GGCTGCTCCA TATTGGCTTG ATGAGCCCAA GAACTTGATT
    CTTGCACCAG GTGAGAACGG ACGTTTGGTA TGTCGTGCCA GTGGCAGCCC CAAACCTACA
    ATCCAATGGT TTATGAATGG AGATGCTACT GAAGTAGGTG CCCCAAATCC CAACTTCGAA
    ATCGCTGGTG ATACCATTAT TTTTAGAGAA GTACAGAGTG GCAACAGCGC TGTATACCAA
    TGCAATGCTT CTAATGAACA TGGTTATCTG TTAGCTAATG CCTTCGTCAG CATTCTGGAT
    GTTCCATCTC GGATGTTGGG CCCTCGTAAT CAGCTGATTC GAGTGATAGA ATACAACAGG
    ACCCAGCTAG ACTGCCCTTT CTTTGGCTCT CCTATACCTA CACTCCGCTG GTTTAAAAAT
    GGACAGGGTA GTAACCTCGA TGGTGGCAAT TACCGGGTGC ATGAAAATGG GACCCTTGAG
    ATTAATACTG TGAGGAAGGA GGACCAAGGC AAATACACAT GTGTTGCTAC CAATATTTTG
    GGCAATGCTG AAAATACTGT GCGGCTGGAG GTCAAAGAGC CAACTCGTAT TGTAAAGGGC
    CCTGAAGATC AGATTGCGAA GAGGGGCTCT TCTGTGCATC TGGACTGTCG AGTGCGCAGT
    GACTCCTCAC TCAGACTCAC TGTTACCTGG ATAAAAGATG ACACTCAGTT GTATATAAAC
    AGCAGAATGA AGAAGGATGA GGATGGACTT ACAATATTCG GTGTGGCAGA AAAGGATCAG
    GGAGCATATA CATGTGTTGC TAAAACTGAA TTAGACCAGG ACTCTTCTAA AGCTTATCTC
    ACGATACAGG AAACTTCCTC ACCTACTAGC CGACTGACCT CCCAGCTTAT AGACAAACCA
    GACCCCCCAC AGGACCTGGA GCTAACTGAA CCATCAGTTA GGAGTGTTCG CCTCACCTGG
    GTTCCAGGGG ATGATAACAA CAGTCCAATT ACAGGTTTCA TTGTACAATT TGAGGAAGAC
    AGGCATCAGC CCGGTACATG GCACAACCTT GCCTCTGTCC CTGCTAATGT GAACTCTGTC
    ATACTGCAGC TTTCTCCTTA TGTCAACTAC CAGTTTCGTG TTCTTGCACT AAATGAACTT
    GGGAGCAGTA TGCCAAGCCC ATCTTCAGAA CGTTTTCAGA CAGCAGGAGC ACCTCCAGAA
    ACTAATCCAG GTGATGTGAA GGGAGCAGGG ACTAGCAAAA ACAATATGGA AATCACATGG
    ACACCACTGA ACTCCACTCA GGCGTATGGT CCAAACCTGC GCTACAACGT GAAGTGGCGC
    CAACGTCACA CAGGAGAACC TTGGAGCAAT ATTACAGTGT GGGACAACAA GCATGTGGTG
    TGGAATACAC CAGTATACAC CCAATATGAG CTAAGAGTTC AGGCGGAAAA TGAATTTGGA
    AAGGCTCCAG AGCCAAGCAC CATGATTGGA TATTCTGGTG AAGACTATCC TAAGGCTGCA
    CCCACTGACA TTAAGCTTCG GGTCATGAAC AGCACCGCCA TCTCTCTTCA GTGGACACGT
    GTGGCACCTG CCTCTATCCA AGGAAGCCTA CTGGAATACA GAGCTTATTT TTGGAGAGAA
    AGTAGTTTGC TGAAGGGTGT CTGGGTCTCA CGAAAGAAGC AACAACAGAG TTTTATGGGG
    GACCGTGTGA GAGGCATCGT ATCTCAACTG ATTCCATACA GCATCTACAA GCTGTACTTG
    GTGGCAGTGA ATGGCCGAGG GGATGGCCCC AAAAGTGATG TTAAGGAATT CACAACCCCA
    GAGGGAGTGC CGAGTGCTCC TAAATACTTT AGAGTATATG AGCCAAACCG GGAAATGATT
    GTTGTTGAAT GGGGTCACCC AGAGCACCCG AACGGAAACT TGACTGCATA CAACCTCCGC
    ATCCAACCAT TTAATGGCAC CAGAACAGGG AAGGCTGTAT TTGAAACTCT TTCACGTAAT
    CAAACCAAAT ATACCCTGCA AAGAACAGAT CCCGTGTCCC GGTATCGGTT TGCTATCAAT
    GCCAGAACGC AGGTTGGTGC AGGGGAAGTC ATCACAGCTG AGTCTCCTGT CAGACTGGAT
    GAAGTTTCTC CCACGTCAGT GTCTTCTAAG TCTCCTGCAC CTGTCACAGA TGCTCCCACA
    GAGGTCCCAA CTATTCCCCC AACCTCATCT GGTGCCACCA CTCTTCTAGC TACAACCAGT
    ACAACCAGTG TCACAACGAC TACTGCTGGT ACAGAAAGTT CGCTGGTCGC CATTACTAGA
    ATTCCTAATA TAGCCACCAC TGGACCATCT CTATGGAACC TAACAGCATT ACCCAACACT
    TACTGGGCAA ACATCACCTG GATGCATAAC TTAGAGCCAG GCTCTGTGTT TATGGTTGAG
    TATACTGACA GTAAGCATCA CTTCTGCATG AGGGGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031752699.1
    CDS548..4006
    Translation

    Target ORF information:

    RefSeq Version XM_031896839.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neurofascin (nfasc), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896839.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGACAGATT TTCTAGAAAG GAGGTTAACG GTGATCCACT ACTACTGTCT CGGAAGCAAC 
    ATGCCATCAT GTTTAGTCCT TCTCCTGCTC TTTCTCCACC TAGTCTGCAC TATTAAAGTT
    CCACTGGACC CAAACATTCA GCGAGACCTA ATACAGCCCC CAACCATCAT CAAGCAGTCA
    GCAAAGAATT ATATTGTAGA TCCCCGTGAT AACATCCTAA TTGAGTGTGA AGCTCAGGGG
    AATCCTTACC CAATGTTTTC ATGGACAAAA AATGGGAAAT TTTACAATGT GGCAAAAGAC
    CCTCGAGTGT CATTCCGTCG ACGATCAGGC ACCCTTGAAA TAGAAATTCA TGGAGGAGGG
    CGGCCAGAGG ACTATGAGGG AGAATACCAA TGCTCTGCCC GCAACCAACA TGGAACAGCT
    TTATCAAATA AGATCTTACT TCAAGTCTCT CGATCCCCTT TGTGGCCCAA AGAAAACCTT
    GACCCGGTGG TGGTAAATGA AGGTGCCTTC TTGATCCTTC AGTGCAATCC CCCACATGGA
    CTTCCTCCTC CAGTCATATT TTGGATGAGC AGCTTGATGG AGCCTATTGT CCAGGACAAG
    AGGGTTTCTC AGGGACTGAA TGGGGACCTT TATTTCTCCA ACGTTCTGCG CCAGGATACT
    CAGACTGATT ATAGCTGCAA TGCACGCTCC CATTTCACAC ACACCATTCA ACAGAAGAAC
    CCATATACTC TAAAAGTGCT TACCACCAGA GGGGTACCTG AGAGAACCCC AACCTTCTTG
    CTTCCCACGG GGACTTCTAG CAGTAAACTG GTGTTACGCG ATGAGGACTT GCTACTGGAG
    TGTATTGCTT CTGGAGTACC AACGCCAGAC ATCATGTGGT ATAAGAAAGG AGGAGAGCTA
    CCCAGCAGGA AGCTGAAGTT TGAAAATTTT AACAAGACTC TACGCATAAT TGGAGTGTCT
    GAAGAGGACT CGGGGGAGTA TTTCTGCCAA GCTTCAAACA AAATGGGCAG CATTCGGCAC
    ACGATTTCTG TTCGGGTTAA GGCTGCTCCA TATTGGCTTG ATGAGCCCAA GAACTTGATT
    CTTGCACCAG GTGAGAACGG ACGTTTGGTA TGTCGTGCCA GTGGCAGCCC CAAACCTACA
    ATCCAATGGT TTATGAATGG AGATGCTACT GAAGTAGGTG CCCCAAATCC CAACTTCGAA
    ATCGCTGGTG ATACCATTAT TTTTAGAGAA GTACAGAGTG GCAACAGCGC TGTATACCAA
    TGCAATGCTT CTAATGAACA TGGTTATCTG TTAGCTAATG CCTTCGTCAG CATTCTGGAT
    GTTCCATCTC GGATGTTGGG CCCTCGTAAT CAGCTGATTC GAGTGATAGA ATACAACAGG
    ACCCAGCTAG ACTGCCCTTT CTTTGGCTCT CCTATACCTA CACTCCGCTG GTTTAAAAAT
    GGACAGGGTA GTAACCTCGA TGGTGGCAAT TACCGGGTGC ATGAAAATGG GACCCTTGAG
    ATTAATACTG TGAGGAAGGA GGACCAAGGC AAATACACAT GTGTTGCTAC CAATATTTTG
    GGCAATGCTG AAAATACTGT GCGGCTGGAG GTCAAAGAGC CAACTCGTAT TGTAAAGGGC
    CCTGAAGATC AGATTGCGAA GAGGGGCTCT TCTGTGCATC TGGACTGTCG AGTGCGCAGT
    GACTCCTCAC TCAGACTCAC TGTTACCTGG ATAAAAGATG ACACTCAGTT GTATATAAAC
    AGCAGAATGA AGAAGGATGA GGATGGACTT ACAATATTCG GTGTGGCAGA AAAGGATCAG
    GGAGCATATA CATGTGTTGC TAAAACTGAA TTAGACCAGG ACTCTTCTAA AGCTTATCTC
    ACGATACAGG AAACTTCCTC ACCTACTAGC CGACTGACCT CCCAGCTTAT AGACAAACCA
    GACCCCCCAC AGGACCTGGA GCTAACTGAA CCATCAGTTA GGAGTGTTCG CCTCACCTGG
    GTTCCAGGGG ATGATAACAA CAGTCCAATT ACAGGTTTCA TTGTACAATT TGAGGAAGAC
    AGGCATCAGC CCGGTACATG GCACAACCTT GCCTCTGTCC CTGCTAATGT GAACTCTGTC
    ATACTGCAGC TTTCTCCTTA TGTCAACTAC CAGTTTCGTG TTCTTGCACT AAATGAACTT
    GGGAGCAGTA TGCCAAGCCC ATCTTCAGAA CGTTTTCAGA CAGCAGGAGC ACCTCCAGAA
    ACTAATCCAG GTGATGTGAA GGGAGCAGGG ACTAGCAAAA ACAATATGGA AATCACATGG
    ACACCACTGA ACTCCACTCA GGCGTATGGT CCAAACCTGC GCTACAACGT GAAGTGGCGC
    CAACGTCACA CAGGAGAACC TTGGAGCAAT ATTACAGTGT GGGACAACAA GCATGTGGTG
    TGGAATACAC CAGTATACAC CCAATATGAG CTAAGAGTTC AGGCGGAAAA TGAATTTGGA
    AAGGCTCCAG AGCCAAGCAC CATGATTGGA TATTCTGGTG AAGACTATCC TAAGGCTGCA
    CCCACTGACA TTAAGCTTCG GGTCATGAAC AGCACCGCCA TCTCTCTTCA GTGGACACGT
    GTGGCACCTG CCTCTATCCA AGGAAGCCTA CTGGAATACA GAGCTTATTT TTGGAGAGAA
    AGTAGTTTGC TGAAGGGTGT CTGGGTCTCA CGAAAGAAGC AACAACAGAG TTTTATGGGG
    GACCGTGTGA GAGGCATCGT ATCTCAACTG ATTCCATACA GCATCTACAA GCTGTACTTG
    GTGGCAGTGA ATGGCCGAGG GGATGGCCCC AAAAGTGATG TTAAGGAATT CACAACCCCA
    GAGGGAGTGC CGAGTGCTCC TAAATACTTT AGAGTATATG AGCCAAACCG GGAAATGATT
    GTTGTTGAAT GGGGTCACCC AGAGCACCCG AACGGAAACT TGACTGCATA CAACCTCCGC
    ATCCAACCAT TTAATGGCAC CAGAACAGGG AAGGCTGTAT TTGAAACTCT TTCACGTAAT
    CAAACCAAAT ATACCCTGCA AAGAACAGAT CCCGTGTCCC GGTATCGGTT TGCTATCAAT
    GCCAGAACGC AGGTTGGTGC AGGGGAAGTC ATCACAGCTG AGTCTCCTGT CAGACTGGAT
    GAAGTTTCTC CCACGTCAGT GTCTTCTAAG TCTCCTGCAC CTGTCACAGA TGCTCCCACA
    GAGGTCCCAA CTATTCCCCC AACCTCATCT GGTGCCACCA CTCTTCTAGC TACAACCAGT
    ACAACCAGTG TCACAACGAC TACTGCTGGT ACAGAAAGTT CGCTGGTCGC CATTACTAGA
    ATTCCTAATA TAGCCACCAC TGGACCATCT CTATGGAACC TAACAGCATT ACCCAACACT
    TACTGGGCAA ACATCACCTG GATGCATAAC TTAGAGCCAG GCTCTGTGTT TATGGTTGAG
    TATACTGACA GTAAGCATCA CTTCTGCATG AGGGGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa03815
    Clone ID Related Accession (Same CDS sequence) XM_031896840.1
    Accession Version XM_031896840.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3252bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neurofascin isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGACAGATT TTCTAGAAAG GAGGTTAACG GTGATCCACT ACTACTGTCT CGGAAGCAAC 
    ATGCCATCAT GTTTAGTCCT TCTCCTGCTC TTTCTCCACC TAGTCTGCAC TATTAAAGTT
    CCACTGGACC CAAACATTCA GCGAGACCTA ATACAGCCCC CAACCATCAT CAAGCAGTCA
    GCAAAGAATT ATATTGTAGA TCCCCGTGAT AACATCCTAA TTGAGTGTGA AGCTCAGGGG
    AATCCTTACC CAATGTTTTC ATGGACAAAA AATGGGAAAT TTTACAATGT GGCAAAAGAC
    CCTCGAGTGT CATTCCGTCG ACGATCAGGC ACCCTTGAAA TAGAAATTCA TGGAGGAGGG
    CGGCCAGAGG ACTATGAGGG AGAATACCAA TGCTCTGCCC GCAACCAACA TGGAACAGCT
    TTATCAAATA AGATCTTACT TCAAGTCTCT CGATCCCCTT TGTGGCCCAA AGAAAACCTT
    GACCCGGTGG TGGTAAATGA AGGTGCCTTC TTGATCCTTC AGTGCAATCC CCCACATGGA
    CTTCCTCCTC CAGTCATATT TTGGATGAGC AGCTTGATGG AGCCTATTGT CCAGGACAAG
    AGGGTTTCTC AGGGACTGAA TGGGGACCTT TATTTCTCCA ACGTTCTGCG CCAGGATACT
    CAGACTGATT ATAGCTGCAA TGCACGCTCC CATTTCACAC ACACCATTCA ACAGAAGAAC
    CCATATACTC TAAAAGTGCT TACCACCAGA GGGGTACCTG AGAGAACCCC AACCTTCTTG
    CTTCCCACGG GGACTTCTAG CAGTAAACTG GTGTTACGCG ATGAGGACTT GCTACTGGAG
    TGTATTGCTT CTGGAGTACC AACGCCAGAC ATCATGTGGT ATAAGAAAGG AGGAGAGCTA
    CCCAGCAGGA AGCTGAAGTT TGAAAATTTT AACAAGACTC TACGCATAAT TGGAGTGTCT
    GAAGAGGACT CGGGGGAGTA TTTCTGCCAA GCTTCAAACA AAATGGGCAG CATTCGGCAC
    ACGATTTCTG TTCGGGTTAA GGCTGCTCCA TATTGGCTTG ATGAGCCCAA GAACTTGATT
    CTTGCACCAG GTGAGAACGG ACGTTTGGTA TGTCGTGCCA GTGGCAGCCC CAAACCTACA
    ATCCAATGGT TTATGAATGG AGATGCTACT GAAGTAGGTG CCCCAAATCC CAACTTCGAA
    ATCGCTGGTG ATACCATTAT TTTTAGAGAA GTACAGAGTG GCAACAGCGC TGTATACCAA
    TGCAATGCTT CTAATGAACA TGGTTATCTG TTAGCTAATG CCTTCGTCAG CATTCTGGAT
    GTTCCATCTC GGATGTTGGG CCCTCGTAAT CAGCTGATTC GAGTGATAGA ATACAACAGG
    ACCCAGCTAG ACTGCCCTTT CTTTGGCTCT CCTATACCTA CACTCCGCTG GTTTAAAAAT
    GGACAGGGTA GTAACCTCGA TGGTGGCAAT TACCGGGTGC ATGAAAATGG GACCCTTGAG
    ATTAATACTG TGAGGAAGGA GGACCAAGGC AAATACACAT GTGTTGCTAC CAATATTTTG
    GGCAATGCTG AAAATACTGT GCGGCTGGAG GTCAAAGAGC CAACTCGTAT TGTAAAGGGC
    CCTGAAGATC AGATTGCGAA GAGGGGCTCT TCTGTGCATC TGGACTGTCG AGTGCGCAGT
    GACTCCTCAC TCAGACTCAC TGTTACCTGG ATAAAAGATG ACACTCAGTT GTATATAAAC
    AGCAGAATGA AGAAGGATGA GGATGGACTT ACAATATTCG GTGTGGCAGA AAAGGATCAG
    GGAGCATATA CATGTGTTGC TAAAACTGAA TTAGACCAGG ACTCTTCTAA AGCTTATCTC
    ACGATACAGG AAACTTCCTC ACCTACTAGC CGACTGACCT CCCAGCTTAT AGACAAACCA
    GACCCCCCAC AGGACCTGGA GCTAACTGAA CCATCAGTTA GGAGTGTTCG CCTCACCTGG
    GTTCCAGGGG ATGATAACAA CAGTCCAATT ACAGGTTTCA TTGTACAATT TGAGGAAGAC
    AGGCATCAGC CCGGTACATG GCACAACCTT GCCTCTGTCC CTGCTAATGT GAACTCTGTC
    ATACTGCAGC TTTCTCCTTA TGTCAACTAC CAGTTTCGTG TTCTTGCACT AAATGAACTT
    GGGAGCAGTA TGCCAAGCCC ATCTTCAGAA CGTTTTCAGA CAGCAGGAGC ACCTCCAGAA
    ACTAATCCAG GTGATGTGAA GGGAGCAGGG ACTAGCAAAA ACAATATGGA AATCACATGG
    ACACCACTGA ACTCCACTCA GGCGTATGGT CCAAACCTGC GCTACAACGT GAAGTGGCGC
    CAACGTCACA CAGGAGAACC TTGGAGCAAT ATTACAGTGT GGGACAACAA GCATGTGGTG
    TGGAATACAC CAGTATACAC CCAATATGAG CTAAGAGTTC AGGCGGAAAA TGAATTTGGA
    AAGGCTCCAG AGCCAAGCAC CATGATTGGA TATTCTGGTG AAGACTTGCC GAGTGCTCCT
    AAATACTTTA GAGTATATGA GCCAAACCGG GAAATGATTG TTGTTGAATG GGGTCACCCA
    GAGCACCCGA ACGGAAACTT GACTGCATAC AACCTCCGCA TCCAACCATT TAATGGCACC
    AGAACAGGGA AGGCTGTATT TGAAACTCTT TCACGTAATC AAACCAAATA TACCCTGCAA
    AGAACAGATC CCGTGTCCCG GTATCGGTTT GCTATCAATG CCAGAACGCA GGTTGGTGCA
    GGGGAAGTCA TCACAGCTGA GTCTCCTGTC AGACTGGATG AAGTTTCTCC CACGTCAGCT
    TACACCAAGA ACCAAGTTGA CATTGCTACA CAAGGCTGGT TTATTGGGCT CATGTGTGCA
    GTTGCTCTAC TGGTACTGAT TTTGCTGATC GTTTGTTTTA TCAAAAGAAG CCGAGGAGGC
    AAATACCCAG TGCGAGAAAA GAAGGAGGTG GCGTTGGGAC CAGATGATAA CAAAGAGGGG
    GATGGCTCAT TTGACTACAG TGATGAGGAC AACAAGCCCC TGCAGGGTAG TCAAACTTCG
    CTGGATGGAA CCATAAAGCA ACAAGAGAGT GATGACAGCC TTGTTGATTA TGGAGAAGGA
    GGAGAGGGGC AGTTTAATGA AGATGGATCG TTTATAGGGC AGTATACAGT GAAAAAGGAC
    AAAGATGAGA CTGAGGGAAA TGAAAGTTCA GAGGCCACAT CTCCTGTAAA TGCTATCTAT
    TCTCTGGCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031752700.1
    CDS543..3794
    Translation

    Target ORF information:

    RefSeq Version XM_031896840.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neurofascin (nfasc), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896840.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGACAGATT TTCTAGAAAG GAGGTTAACG GTGATCCACT ACTACTGTCT CGGAAGCAAC 
    ATGCCATCAT GTTTAGTCCT TCTCCTGCTC TTTCTCCACC TAGTCTGCAC TATTAAAGTT
    CCACTGGACC CAAACATTCA GCGAGACCTA ATACAGCCCC CAACCATCAT CAAGCAGTCA
    GCAAAGAATT ATATTGTAGA TCCCCGTGAT AACATCCTAA TTGAGTGTGA AGCTCAGGGG
    AATCCTTACC CAATGTTTTC ATGGACAAAA AATGGGAAAT TTTACAATGT GGCAAAAGAC
    CCTCGAGTGT CATTCCGTCG ACGATCAGGC ACCCTTGAAA TAGAAATTCA TGGAGGAGGG
    CGGCCAGAGG ACTATGAGGG AGAATACCAA TGCTCTGCCC GCAACCAACA TGGAACAGCT
    TTATCAAATA AGATCTTACT TCAAGTCTCT CGATCCCCTT TGTGGCCCAA AGAAAACCTT
    GACCCGGTGG TGGTAAATGA AGGTGCCTTC TTGATCCTTC AGTGCAATCC CCCACATGGA
    CTTCCTCCTC CAGTCATATT TTGGATGAGC AGCTTGATGG AGCCTATTGT CCAGGACAAG
    AGGGTTTCTC AGGGACTGAA TGGGGACCTT TATTTCTCCA ACGTTCTGCG CCAGGATACT
    CAGACTGATT ATAGCTGCAA TGCACGCTCC CATTTCACAC ACACCATTCA ACAGAAGAAC
    CCATATACTC TAAAAGTGCT TACCACCAGA GGGGTACCTG AGAGAACCCC AACCTTCTTG
    CTTCCCACGG GGACTTCTAG CAGTAAACTG GTGTTACGCG ATGAGGACTT GCTACTGGAG
    TGTATTGCTT CTGGAGTACC AACGCCAGAC ATCATGTGGT ATAAGAAAGG AGGAGAGCTA
    CCCAGCAGGA AGCTGAAGTT TGAAAATTTT AACAAGACTC TACGCATAAT TGGAGTGTCT
    GAAGAGGACT CGGGGGAGTA TTTCTGCCAA GCTTCAAACA AAATGGGCAG CATTCGGCAC
    ACGATTTCTG TTCGGGTTAA GGCTGCTCCA TATTGGCTTG ATGAGCCCAA GAACTTGATT
    CTTGCACCAG GTGAGAACGG ACGTTTGGTA TGTCGTGCCA GTGGCAGCCC CAAACCTACA
    ATCCAATGGT TTATGAATGG AGATGCTACT GAAGTAGGTG CCCCAAATCC CAACTTCGAA
    ATCGCTGGTG ATACCATTAT TTTTAGAGAA GTACAGAGTG GCAACAGCGC TGTATACCAA
    TGCAATGCTT CTAATGAACA TGGTTATCTG TTAGCTAATG CCTTCGTCAG CATTCTGGAT
    GTTCCATCTC GGATGTTGGG CCCTCGTAAT CAGCTGATTC GAGTGATAGA ATACAACAGG
    ACCCAGCTAG ACTGCCCTTT CTTTGGCTCT CCTATACCTA CACTCCGCTG GTTTAAAAAT
    GGACAGGGTA GTAACCTCGA TGGTGGCAAT TACCGGGTGC ATGAAAATGG GACCCTTGAG
    ATTAATACTG TGAGGAAGGA GGACCAAGGC AAATACACAT GTGTTGCTAC CAATATTTTG
    GGCAATGCTG AAAATACTGT GCGGCTGGAG GTCAAAGAGC CAACTCGTAT TGTAAAGGGC
    CCTGAAGATC AGATTGCGAA GAGGGGCTCT TCTGTGCATC TGGACTGTCG AGTGCGCAGT
    GACTCCTCAC TCAGACTCAC TGTTACCTGG ATAAAAGATG ACACTCAGTT GTATATAAAC
    AGCAGAATGA AGAAGGATGA GGATGGACTT ACAATATTCG GTGTGGCAGA AAAGGATCAG
    GGAGCATATA CATGTGTTGC TAAAACTGAA TTAGACCAGG ACTCTTCTAA AGCTTATCTC
    ACGATACAGG AAACTTCCTC ACCTACTAGC CGACTGACCT CCCAGCTTAT AGACAAACCA
    GACCCCCCAC AGGACCTGGA GCTAACTGAA CCATCAGTTA GGAGTGTTCG CCTCACCTGG
    GTTCCAGGGG ATGATAACAA CAGTCCAATT ACAGGTTTCA TTGTACAATT TGAGGAAGAC
    AGGCATCAGC CCGGTACATG GCACAACCTT GCCTCTGTCC CTGCTAATGT GAACTCTGTC
    ATACTGCAGC TTTCTCCTTA TGTCAACTAC CAGTTTCGTG TTCTTGCACT AAATGAACTT
    GGGAGCAGTA TGCCAAGCCC ATCTTCAGAA CGTTTTCAGA CAGCAGGAGC ACCTCCAGAA
    ACTAATCCAG GTGATGTGAA GGGAGCAGGG ACTAGCAAAA ACAATATGGA AATCACATGG
    ACACCACTGA ACTCCACTCA GGCGTATGGT CCAAACCTGC GCTACAACGT GAAGTGGCGC
    CAACGTCACA CAGGAGAACC TTGGAGCAAT ATTACAGTGT GGGACAACAA GCATGTGGTG
    TGGAATACAC CAGTATACAC CCAATATGAG CTAAGAGTTC AGGCGGAAAA TGAATTTGGA
    AAGGCTCCAG AGCCAAGCAC CATGATTGGA TATTCTGGTG AAGACTTGCC GAGTGCTCCT
    AAATACTTTA GAGTATATGA GCCAAACCGG GAAATGATTG TTGTTGAATG GGGTCACCCA
    GAGCACCCGA ACGGAAACTT GACTGCATAC AACCTCCGCA TCCAACCATT TAATGGCACC
    AGAACAGGGA AGGCTGTATT TGAAACTCTT TCACGTAATC AAACCAAATA TACCCTGCAA
    AGAACAGATC CCGTGTCCCG GTATCGGTTT GCTATCAATG CCAGAACGCA GGTTGGTGCA
    GGGGAAGTCA TCACAGCTGA GTCTCCTGTC AGACTGGATG AAGTTTCTCC CACGTCAGCT
    TACACCAAGA ACCAAGTTGA CATTGCTACA CAAGGCTGGT TTATTGGGCT CATGTGTGCA
    GTTGCTCTAC TGGTACTGAT TTTGCTGATC GTTTGTTTTA TCAAAAGAAG CCGAGGAGGC
    AAATACCCAG TGCGAGAAAA GAAGGAGGTG GCGTTGGGAC CAGATGATAA CAAAGAGGGG
    GATGGCTCAT TTGACTACAG TGATGAGGAC AACAAGCCCC TGCAGGGTAG TCAAACTTCG
    CTGGATGGAA CCATAAAGCA ACAAGAGAGT GATGACAGCC TTGTTGATTA TGGAGAAGGA
    GGAGAGGGGC AGTTTAATGA AGATGGATCG TTTATAGGGC AGTATACAGT GAAAAAGGAC
    AAAGATGAGA CTGAGGGAAA TGAAAGTTCA GAGGCCACAT CTCCTGTAAA TGCTATCTAT
    TCTCTGGCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa03816
    Clone ID Related Accession (Same CDS sequence) XM_031896841.1
    Accession Version XM_031896841.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3237bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neurofascin isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGACAGATT TTCTAGAAAG GAGGTTAACG GTGATCCACT ACTACTGTCT CGGAAGCAAC 
    ATGCCATCAT GTTTAGTCCT TCTCCTGCTC TTTCTCCACC TAGTCTGCAC TATTAAAGTT
    CCACTGGACC CAAACATTCA GCGAGACCTA ATACAGCCCC CAACCATCAT CAAGCAGTCA
    GCAAAGAATT ATATTGTAGA TCCCCGTGAT AACATCCTAA TTGAGTGTGA AGCTCAGGGG
    AATCCTTACC CAATGTTTTC ATGGACAAAA AATGGGAAAT TTTACAATGT GGCAAAAGAC
    CCTCGAGTGT CATTCCGTCG ACGATCAGGC ACCCTTGAAA TAGAAATTCA TGGAGGAGGG
    CGGCCAGAGG ACTATGAGGG AGAATACCAA TGCTCTGCCC GCAACCAACA TGGAACAGCT
    TTATCAAATA AGATCTTACT TCAAGTCTCT CGATCCCCTT TGTGGCCCAA AGAAAACCTT
    GACCCGGTGG TGGTAAATGA AGGTGCCTTC TTGATCCTTC AGTGCAATCC CCCACATGGA
    CTTCCTCCTC CAGTCATATT TTGGATGAGC AGCTTGATGG AGCCTATTGT CCAGGACAAG
    AGGGTTTCTC AGGGACTGAA TGGGGACCTT TATTTCTCCA ACGTTCTGCG CCAGGATACT
    CAGACTGATT ATAGCTGCAA TGCACGCTCC CATTTCACAC ACACCATTCA ACAGAAGAAC
    CCATATACTC TAAAAGTGCT TACCACCAGA GGGGTACCTG AGAGAACCCC AACCTTCTTG
    CTTCCCACGG GGACTTCTAG CAGTAAACTG GTGTTACGCG ATGAGGACTT GCTACTGGAG
    TGTATTGCTT CTGGAGTACC AACGCCAGAC ATCATGTGGT ATAAGAAAGG AGGAGAGCTA
    CCCAGCAGGA AGCTGAAGTT TGAAAATTTT AACAAGACTC TACGCATAAT TGGAGTGTCT
    GAAGAGGACT CGGGGGAGTA TTTCTGCCAA GCTTCAAACA AAATGGGCAG CATTCGGCAC
    ACGATTTCTG TTCGGGTTAA GGCTGCTCCA TATTGGCTTG ATGAGCCCAA GAACTTGATT
    CTTGCACCAG GTGAGAACGG ACGTTTGGTA TGTCGTGCCA GTGGCAGCCC CAAACCTACA
    ATCCAATGGT TTATGAATGG AGATGCTACT GAAGTAGGTG CCCCAAATCC CAACTTCGAA
    ATCGCTGGTG ATACCATTAT TTTTAGAGAA GTACAGAGTG GCAACAGCGC TGTATACCAA
    TGCAATGCTT CTAATGAACA TGGTTATCTG TTAGCTAATG CCTTCGTCAG CATTCTGGAT
    GTTCCATCTC GGATGTTGGG CCCTCGTAAT CAGCTGATTC GAGTGATAGA ATACAACAGG
    ACCCAGCTAG ACTGCCCTTT CTTTGGCTCT CCTATACCTA CACTCCGCTG GTTTAAAAAT
    GGACAGGGTA GTAACCTCGA TGGTGGCAAT TACCGGGTGC ATGAAAATGG GACCCTTGAG
    ATTAATACTG TGAGGAAGGA GGACCAAGGC AAATACACAT GTGTTGCTAC CAATATTTTG
    GGCAATGCTG AAAATACTGT GCGGCTGGAG GTCAAAGAGC CAACTCGTAT TGTAAAGGGC
    CCTGAAGATC AGATTGCGAA GAGGGGCTCT TCTGTGCATC TGGACTGTCG AGTGCGCAGT
    GACTCCTCAC TCAGACTCAC TGTTACCTGG ATAAAAGATG ACACTCAGTT GTATATAAAC
    AGCAGAATGA AGAAGGATGA GGATGGACTT ACAATATTCG GTGTGGCAGA AAAGGATCAG
    GGAGCATATA CATGTGTTGC TAAAACTGAA TTAGACCAGG ACTCTTCTAA AGCTTATCTC
    ACGATACAGG AAACTTCCTC ACCTACTAGC CGACTGACCT CCCAGCTTAT AGACAAACCA
    GACCCCCCAC AGGACCTGGA GCTAACTGAA CCATCAGTTA GGAGTGTTCG CCTCACCTGG
    GTTCCAGGGG ATGATAACAA CAGTCCAATT ACAGGTTTCA TTGTACAATT TGAGGAAGAC
    AGGCATCAGC CCGGTACATG GCACAACCTT GCCTCTGTCC CTGCTAATGT GAACTCTGTC
    ATACTGCAGC TTTCTCCTTA TGTCAACTAC CAGTTTCGTG TTCTTGCACT AAATGAACTT
    GGGAGCAGTA TGCCAAGCCC ATCTTCAGAA CGTTTTCAGA CAGCAGGAGC ACCTCCAGAA
    ACTAATCCAG GTGATGTGAA GGGAGCAGGG ACTAGCAAAA ACAATATGGA AATCACATGG
    ACACCACTGA ACTCCACTCA GGCGTATGGT CCAAACCTGC GCTACAACGT GAAGTGGCGC
    CAACGTCACA CAGGAGAACC TTGGAGCAAT ATTACAGTGT GGGACAACAA GCATGTGGTG
    TGGAATACAC CAGTATACAC CCAATATGAG CTAAGAGTTC AGGCGGAAAA TGAATTTGGA
    AAGGCTCCAG AGCCAAGCAC CATGATTGGA TATTCTGGTG AAGACTTGCC GAGTGCTCCT
    AAATACTTTA GAGTATATGA GCCAAACCGG GAAATGATTG TTGTTGAATG GGGTCACCCA
    GAGCACCCGA ACGGAAACTT GACTGCATAC AACCTCCGCA TCCAACCATT TAATGGCACC
    AGAACAGGGA AGGCTGTATT TGAAACTCTT TCACGTAATC AAACCAAATA TACCCTGCAA
    AGAACAGATC CCGTGTCCCG GTATCGGTTT GCTATCAATG CCAGAACGCA GGTTGGTGCA
    GGGGAAGTCA TCACAGCTGA GTCTCCTGTC AGACTGGATG AAGCTTACAC CAAGAACCAA
    GTTGACATTG CTACACAAGG CTGGTTTATT GGGCTCATGT GTGCAGTTGC TCTACTGGTA
    CTGATTTTGC TGATCGTTTG TTTTATCAAA AGAAGCCGAG GAGGCAAATA CCCAGTGCGA
    GAAAAGAAGG AGGTGGCGTT GGGACCAGAT GATAACAAAG AGGGGGATGG CTCATTTGAC
    TACAGTGATG AGGACAACAA GCCCCTGCAG GGTAGTCAAA CTTCGCTGGA TGGAACCATA
    AAGCAACAAG AGAGTGATGA CAGCCTTGTT GATTATGGAG AAGGAGGAGA GGGGCAGTTT
    AATGAAGATG GATCGTTTAT AGGGCAGTAT ACAGTGAAAA AGGACAAAGA TGAGACTGAG
    GGAAATGAAA GTTCAGAGGC CACATCTCCT GTAAATGCTA TCTATTCTCT GGCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031752701.1
    CDS543..3779
    Translation

    Target ORF information:

    RefSeq Version XM_031896841.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neurofascin (nfasc), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896841.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGACAGATT TTCTAGAAAG GAGGTTAACG GTGATCCACT ACTACTGTCT CGGAAGCAAC 
    ATGCCATCAT GTTTAGTCCT TCTCCTGCTC TTTCTCCACC TAGTCTGCAC TATTAAAGTT
    CCACTGGACC CAAACATTCA GCGAGACCTA ATACAGCCCC CAACCATCAT CAAGCAGTCA
    GCAAAGAATT ATATTGTAGA TCCCCGTGAT AACATCCTAA TTGAGTGTGA AGCTCAGGGG
    AATCCTTACC CAATGTTTTC ATGGACAAAA AATGGGAAAT TTTACAATGT GGCAAAAGAC
    CCTCGAGTGT CATTCCGTCG ACGATCAGGC ACCCTTGAAA TAGAAATTCA TGGAGGAGGG
    CGGCCAGAGG ACTATGAGGG AGAATACCAA TGCTCTGCCC GCAACCAACA TGGAACAGCT
    TTATCAAATA AGATCTTACT TCAAGTCTCT CGATCCCCTT TGTGGCCCAA AGAAAACCTT
    GACCCGGTGG TGGTAAATGA AGGTGCCTTC TTGATCCTTC AGTGCAATCC CCCACATGGA
    CTTCCTCCTC CAGTCATATT TTGGATGAGC AGCTTGATGG AGCCTATTGT CCAGGACAAG
    AGGGTTTCTC AGGGACTGAA TGGGGACCTT TATTTCTCCA ACGTTCTGCG CCAGGATACT
    CAGACTGATT ATAGCTGCAA TGCACGCTCC CATTTCACAC ACACCATTCA ACAGAAGAAC
    CCATATACTC TAAAAGTGCT TACCACCAGA GGGGTACCTG AGAGAACCCC AACCTTCTTG
    CTTCCCACGG GGACTTCTAG CAGTAAACTG GTGTTACGCG ATGAGGACTT GCTACTGGAG
    TGTATTGCTT CTGGAGTACC AACGCCAGAC ATCATGTGGT ATAAGAAAGG AGGAGAGCTA
    CCCAGCAGGA AGCTGAAGTT TGAAAATTTT AACAAGACTC TACGCATAAT TGGAGTGTCT
    GAAGAGGACT CGGGGGAGTA TTTCTGCCAA GCTTCAAACA AAATGGGCAG CATTCGGCAC
    ACGATTTCTG TTCGGGTTAA GGCTGCTCCA TATTGGCTTG ATGAGCCCAA GAACTTGATT
    CTTGCACCAG GTGAGAACGG ACGTTTGGTA TGTCGTGCCA GTGGCAGCCC CAAACCTACA
    ATCCAATGGT TTATGAATGG AGATGCTACT GAAGTAGGTG CCCCAAATCC CAACTTCGAA
    ATCGCTGGTG ATACCATTAT TTTTAGAGAA GTACAGAGTG GCAACAGCGC TGTATACCAA
    TGCAATGCTT CTAATGAACA TGGTTATCTG TTAGCTAATG CCTTCGTCAG CATTCTGGAT
    GTTCCATCTC GGATGTTGGG CCCTCGTAAT CAGCTGATTC GAGTGATAGA ATACAACAGG
    ACCCAGCTAG ACTGCCCTTT CTTTGGCTCT CCTATACCTA CACTCCGCTG GTTTAAAAAT
    GGACAGGGTA GTAACCTCGA TGGTGGCAAT TACCGGGTGC ATGAAAATGG GACCCTTGAG
    ATTAATACTG TGAGGAAGGA GGACCAAGGC AAATACACAT GTGTTGCTAC CAATATTTTG
    GGCAATGCTG AAAATACTGT GCGGCTGGAG GTCAAAGAGC CAACTCGTAT TGTAAAGGGC
    CCTGAAGATC AGATTGCGAA GAGGGGCTCT TCTGTGCATC TGGACTGTCG AGTGCGCAGT
    GACTCCTCAC TCAGACTCAC TGTTACCTGG ATAAAAGATG ACACTCAGTT GTATATAAAC
    AGCAGAATGA AGAAGGATGA GGATGGACTT ACAATATTCG GTGTGGCAGA AAAGGATCAG
    GGAGCATATA CATGTGTTGC TAAAACTGAA TTAGACCAGG ACTCTTCTAA AGCTTATCTC
    ACGATACAGG AAACTTCCTC ACCTACTAGC CGACTGACCT CCCAGCTTAT AGACAAACCA
    GACCCCCCAC AGGACCTGGA GCTAACTGAA CCATCAGTTA GGAGTGTTCG CCTCACCTGG
    GTTCCAGGGG ATGATAACAA CAGTCCAATT ACAGGTTTCA TTGTACAATT TGAGGAAGAC
    AGGCATCAGC CCGGTACATG GCACAACCTT GCCTCTGTCC CTGCTAATGT GAACTCTGTC
    ATACTGCAGC TTTCTCCTTA TGTCAACTAC CAGTTTCGTG TTCTTGCACT AAATGAACTT
    GGGAGCAGTA TGCCAAGCCC ATCTTCAGAA CGTTTTCAGA CAGCAGGAGC ACCTCCAGAA
    ACTAATCCAG GTGATGTGAA GGGAGCAGGG ACTAGCAAAA ACAATATGGA AATCACATGG
    ACACCACTGA ACTCCACTCA GGCGTATGGT CCAAACCTGC GCTACAACGT GAAGTGGCGC
    CAACGTCACA CAGGAGAACC TTGGAGCAAT ATTACAGTGT GGGACAACAA GCATGTGGTG
    TGGAATACAC CAGTATACAC CCAATATGAG CTAAGAGTTC AGGCGGAAAA TGAATTTGGA
    AAGGCTCCAG AGCCAAGCAC CATGATTGGA TATTCTGGTG AAGACTTGCC GAGTGCTCCT
    AAATACTTTA GAGTATATGA GCCAAACCGG GAAATGATTG TTGTTGAATG GGGTCACCCA
    GAGCACCCGA ACGGAAACTT GACTGCATAC AACCTCCGCA TCCAACCATT TAATGGCACC
    AGAACAGGGA AGGCTGTATT TGAAACTCTT TCACGTAATC AAACCAAATA TACCCTGCAA
    AGAACAGATC CCGTGTCCCG GTATCGGTTT GCTATCAATG CCAGAACGCA GGTTGGTGCA
    GGGGAAGTCA TCACAGCTGA GTCTCCTGTC AGACTGGATG AAGCTTACAC CAAGAACCAA
    GTTGACATTG CTACACAAGG CTGGTTTATT GGGCTCATGT GTGCAGTTGC TCTACTGGTA
    CTGATTTTGC TGATCGTTTG TTTTATCAAA AGAAGCCGAG GAGGCAAATA CCCAGTGCGA
    GAAAAGAAGG AGGTGGCGTT GGGACCAGAT GATAACAAAG AGGGGGATGG CTCATTTGAC
    TACAGTGATG AGGACAACAA GCCCCTGCAG GGTAGTCAAA CTTCGCTGGA TGGAACCATA
    AAGCAACAAG AGAGTGATGA CAGCCTTGTT GATTATGGAG AAGGAGGAGA GGGGCAGTTT
    AATGAAGATG GATCGTTTAT AGGGCAGTAT ACAGTGAAAA AGGACAAAGA TGAGACTGAG
    GGAAATGAAA GTTCAGAGGC CACATCTCCT GTAAATGCTA TCTATTCTCT GGCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa33490
    Clone ID Related Accession (Same CDS sequence) XM_031896830.1
    Accession Version XM_031896830.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4014bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neurofascin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGACAGATT TTCTAGAAAG GAGGTTAACG GTGATCCACT ACTACTGTCT CGGAAGCAAC 
    ATGCCATCAT GTTTAGTCCT TCTCCTGCTC TTTCTCCACC TAGTCTGCAC TATTAAAGTT
    CCACTGGACC CAAACATTCA GCGAGACCTA ATACAGCCCC CAACCATCAT CAAGCAGTCA
    GCAAAGAATT ATATTGTAGA TCCCCGTGAT AACATCCTAA TTGAGTGTGA AGCTCAGGGG
    AATCCTTACC CAATGTTTTC ATGGACAAAA AATGGGAAAT TTTACAATGT GGCAAAAGAC
    CCTCGAGTGT CATTCCGTCG ACGATCAGGC ACCCTTGAAA TAGAAATTCA TGGAGGAGGG
    CGGCCAGAGG ACTATGAGGG AGAATACCAA TGCTCTGCCC GCAACCAACA TGGAACAGCT
    TTATCAAATA AGATCTTACT TCAAGTCTCT CGATCCCCTT TGTGGCCCAA AGAAAACCTT
    GACCCGGTGG TGGTAAATGA AGGTGCCTTC TTGATCCTTC AGTGCAATCC CCCACATGGA
    CTTCCTCCTC CAGTCATATT TTGGATGAGC AGCTTGATGG AGCCTATTGT CCAGGACAAG
    AGGGTTTCTC AGGGACTGAA TGGGGACCTT TATTTCTCCA ACGTTCTGCG CCAGGATACT
    CAGACTGATT ATAGCTGCAA TGCACGCTCC CATTTCACAC ACACCATTCA ACAGAAGAAC
    CCATATACTC TAAAAGTGCT TACCACCAGA GGGGTACCTG AGAGAACCCC AACCTTCTTG
    CTTCCCACGG GGACTTCTAG CAGTAAACTG GTGTTACGCG ATGAGGACTT GCTACTGGAG
    TGTATTGCTT CTGGAGTACC AACGCCAGAC ATCATGTGGT ATAAGAAAGG AGGAGAGCTA
    CCCAGCAGGA AGCTGAAGTT TGAAAATTTT AACAAGACTC TACGCATAAT TGGAGTGTCT
    GAAGAGGACT CGGGGGAGTA TTTCTGCCAA GCTTCAAACA AAATGGGCAG CATTCGGCAC
    ACGATTTCTG TTCGGGTTAA GGCTGCTCCA TATTGGCTTG ATGAGCCCAA GAACTTGATT
    CTTGCACCAG GTGAGAACGG ACGTTTGGTA TGTCGTGCCA GTGGCAGCCC CAAACCTACA
    ATCCAATGGT TTATGAATGG AGATGCTACT GAAGGTGCCC CAAATCCCAA CTTCGAAATC
    GCTGGTGATA CCATTATTTT TAGAGAAGTA CAGAGTGGCA ACAGCGCTGT ATACCAATGC
    AATGCTTCTA ATGAACATGG TTATCTGTTA GCTAATGCCT TCGTCAGCAT TCTGGATGTT
    CCATCTCGGA TGTTGGGCCC TCGTAATCAG CTGATTCGAG TGATAGAATA CAACAGGACC
    CAGCTAGACT GCCCTTTCTT TGGCTCTCCT ATACCTACAC TCCGCTGGTT TAAAAATGGA
    CAGGGTAGTA ACCTCGATGG TGGCAATTAC CGGGTGCATG AAAATGGGAC CCTTGAGATT
    AATACTGTGA GGAAGGAGGA CCAAGGCAAA TACACATGTG TTGCTACCAA TATTTTGGGC
    AATGCTGAAA ATACTGTGCG GCTGGAGGTC AAAGAGCCAA CTCGTATTGT AAAGGGCCCT
    GAAGATCAGA TTGCGAAGAG GGGCTCTTCT GTGCATCTGG ACTGTCGAGT GCGCAGTGAC
    TCCTCACTCA GACTCACTGT TACCTGGATA AAAGATGACA CTCAGTTGTA TATAAACAGC
    AGAATGAAGA AGGATGAGGA TGGACTTACA ATATTCGGTG TGGCAGAAAA GGATCAGGGA
    GCATATACAT GTGTTGCTAA AACTGAATTA GACCAGGACT CTTCTAAAGC TTATCTCACG
    ATACAGGAAA CTTCCTCACC TACTAGCCGA CTGACCTCCC AGCTTATAGA CAAACCAGAC
    CCCCCACAGG ACCTGGAGCT AACTGAACCA TCAGTTAGGA GTGTTCGCCT CACCTGGGTT
    CCAGGGGATG ATAACAACAG TCCAATTACA GGTTTCATTG TACAATTTGA GGAAGACAGG
    CATCAGCCCG GTACATGGCA CAACCTTGCC TCTGTCCCTG CTAATGTGAA CTCTGTCATA
    CTGCAGCTTT CTCCTTATGT CAACTACCAG TTTCGTGTTC TTGCACTAAA TGAACTTGGG
    AGCAGTATGC CAAGCCCATC TTCAGAACGT TTTCAGACAG CAGGAGCACC TCCAGAAACT
    AATCCAGGTG ATGTGAAGGG AGCAGGGACT AGCAAAAACA ATATGGAAAT CACATGGACA
    CCACTGAACT CCACTCAGGC GTATGGTCCA AACCTGCGCT ACAACGTGAA GTGGCGCCAA
    CGTCACACAG GAGAACCTTG GAGCAATATT ACAGTGTGGG ACAACAAGCA TGTGGTGTGG
    AATACACCAG TATACACCCA ATATGAGCTA AGAGTTCAGG CGGAAAATGA ATTTGGAAAG
    GCTCCAGAGC CAAGCACCAT GATTGGATAT TCTGGTGAAG ACTATCCTAA GGCTGCACCC
    ACTGACATTA AGCTTCGGGT CATGAACAGC ACCGCCATCT CTCTTCAGTG GACACGTGTG
    GCACCTGCCT CTATCCAAGG AAGCCTACTG GAATACAGAG CTTATTTTTG GAGAGAAAGT
    AGTTTGCTGA AGGGTGTCTG GGTCTCACGA AAGAAGCAAC AACAGAGTTT TATGGGGGAC
    CGTGTGAGAG GCATCGTATC TCAACTGATT CCATACAGCA TCTACAAGCT GTACTTGGTG
    GCAGTGAATG GCCGAGGGGA TGGCCCCAAA AGTGATGTTA AGGAATTCAC AACCCCAGAG
    GGAGTGCCGA GTGCTCCTAA ATACTTTAGA GTATATGAGC CAAACCGGGA AATGATTGTT
    GTTGAATGGG GTCACCCAGA GCACCCGAAC GGAAACTTGA CTGCATACAA CCTCCGCATC
    CAACCATTTA ATGGCACCAG AACAGGGAAG GCTGTATTTG AAACTCTTTC ACGTAATCAA
    ACCAAATATA CCCTGCAAAG AACAGATCCC GTGTCCCGGT ATCGGTTTGC TATCAATGCC
    AGAACGCAGG TTGGTGCAGG GGAAGTCATC ACAGCTGAGT CTCCTGTCAG ACTGGATGAA
    GTTTCTCCCA CGTCAGTGTC TTCTAAGTCT CCTGCACCTG TCACAGATGC TCCCACAGAG
    GTCCCAACTA TTCCCCCAAC CTCATCTGGT GCCACCACTC TTCTAGCTAC AACCAGTACA
    ACCAGTGTCA CAACGACTAC TGCTGGTACA GAAAGTTCGC TGGTCGCCAT TACTAGAATT
    CCTAATATAG CCACCACTGG ACCATCTCTA TGGAACCTAA CAGCATTACC CAACACTTAC
    TGGGCAAACA TCACCTGGAT GCATAACTTA GAGCCAGGCT CTGTGTTTAT GGTTGAGTAT
    ACTGACAGTA ATAATACAGT GAAAACTGTT CCTGTTAAGG CTCATGTCCC TTCTATCCAA
    CTGTTTGATC TGGTGCCTGG GATGACCTAC AGGGTGCGGG TTTACTCCCA GGAGCACCCG
    TCAGTCACAA GCCTATATGT GACATTTGAT ACCAGCTCAG CTTACACCAA GAACCAAGTT
    GACATTGCTA CACAAGGCTG GTTTATTGGG CTCATGTGTG CAGTTGCTCT ACTGGTACTG
    ATTTTGCTGA TCGTTTGTTT TATCAAAAGA AGCCGAGGAG GCAAATACCC AGTGCGAGAA
    AAGAAGGAGG TGGCGTTGGG ACCAGATGAT AACAAAGAGG GGGATGGCTC ATTTGACTAC
    AGTGATGAGG ACAACAAGCC CCTGCAGGGT AGTCAAACTT CGCTGGATGG AACCATAAAG
    CAACAAGAGA GTGATGACAG CCTTGTTGAT TATGGAGAAG GAGGAGAGGG GCAGTTTAAT
    GAAGATGGAT CGTTTATAGG GCAGTATACA GTGAAAAAGG ACAAAGATGA GACTGAGGGA
    AATGAAAGTT CAGAGGCCAC ATCTCCTGTA AATGCTATCT ATTCTCTGGC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031752690.1
    CDS547..4560
    Translation

    Target ORF information:

    RefSeq Version XM_031896830.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neurofascin (nfasc), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896830.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGACAGATT TTCTAGAAAG GAGGTTAACG GTGATCCACT ACTACTGTCT CGGAAGCAAC 
    ATGCCATCAT GTTTAGTCCT TCTCCTGCTC TTTCTCCACC TAGTCTGCAC TATTAAAGTT
    CCACTGGACC CAAACATTCA GCGAGACCTA ATACAGCCCC CAACCATCAT CAAGCAGTCA
    GCAAAGAATT ATATTGTAGA TCCCCGTGAT AACATCCTAA TTGAGTGTGA AGCTCAGGGG
    AATCCTTACC CAATGTTTTC ATGGACAAAA AATGGGAAAT TTTACAATGT GGCAAAAGAC
    CCTCGAGTGT CATTCCGTCG ACGATCAGGC ACCCTTGAAA TAGAAATTCA TGGAGGAGGG
    CGGCCAGAGG ACTATGAGGG AGAATACCAA TGCTCTGCCC GCAACCAACA TGGAACAGCT
    TTATCAAATA AGATCTTACT TCAAGTCTCT CGATCCCCTT TGTGGCCCAA AGAAAACCTT
    GACCCGGTGG TGGTAAATGA AGGTGCCTTC TTGATCCTTC AGTGCAATCC CCCACATGGA
    CTTCCTCCTC CAGTCATATT TTGGATGAGC AGCTTGATGG AGCCTATTGT CCAGGACAAG
    AGGGTTTCTC AGGGACTGAA TGGGGACCTT TATTTCTCCA ACGTTCTGCG CCAGGATACT
    CAGACTGATT ATAGCTGCAA TGCACGCTCC CATTTCACAC ACACCATTCA ACAGAAGAAC
    CCATATACTC TAAAAGTGCT TACCACCAGA GGGGTACCTG AGAGAACCCC AACCTTCTTG
    CTTCCCACGG GGACTTCTAG CAGTAAACTG GTGTTACGCG ATGAGGACTT GCTACTGGAG
    TGTATTGCTT CTGGAGTACC AACGCCAGAC ATCATGTGGT ATAAGAAAGG AGGAGAGCTA
    CCCAGCAGGA AGCTGAAGTT TGAAAATTTT AACAAGACTC TACGCATAAT TGGAGTGTCT
    GAAGAGGACT CGGGGGAGTA TTTCTGCCAA GCTTCAAACA AAATGGGCAG CATTCGGCAC
    ACGATTTCTG TTCGGGTTAA GGCTGCTCCA TATTGGCTTG ATGAGCCCAA GAACTTGATT
    CTTGCACCAG GTGAGAACGG ACGTTTGGTA TGTCGTGCCA GTGGCAGCCC CAAACCTACA
    ATCCAATGGT TTATGAATGG AGATGCTACT GAAGGTGCCC CAAATCCCAA CTTCGAAATC
    GCTGGTGATA CCATTATTTT TAGAGAAGTA CAGAGTGGCA ACAGCGCTGT ATACCAATGC
    AATGCTTCTA ATGAACATGG TTATCTGTTA GCTAATGCCT TCGTCAGCAT TCTGGATGTT
    CCATCTCGGA TGTTGGGCCC TCGTAATCAG CTGATTCGAG TGATAGAATA CAACAGGACC
    CAGCTAGACT GCCCTTTCTT TGGCTCTCCT ATACCTACAC TCCGCTGGTT TAAAAATGGA
    CAGGGTAGTA ACCTCGATGG TGGCAATTAC CGGGTGCATG AAAATGGGAC CCTTGAGATT
    AATACTGTGA GGAAGGAGGA CCAAGGCAAA TACACATGTG TTGCTACCAA TATTTTGGGC
    AATGCTGAAA ATACTGTGCG GCTGGAGGTC AAAGAGCCAA CTCGTATTGT AAAGGGCCCT
    GAAGATCAGA TTGCGAAGAG GGGCTCTTCT GTGCATCTGG ACTGTCGAGT GCGCAGTGAC
    TCCTCACTCA GACTCACTGT TACCTGGATA AAAGATGACA CTCAGTTGTA TATAAACAGC
    AGAATGAAGA AGGATGAGGA TGGACTTACA ATATTCGGTG TGGCAGAAAA GGATCAGGGA
    GCATATACAT GTGTTGCTAA AACTGAATTA GACCAGGACT CTTCTAAAGC TTATCTCACG
    ATACAGGAAA CTTCCTCACC TACTAGCCGA CTGACCTCCC AGCTTATAGA CAAACCAGAC
    CCCCCACAGG ACCTGGAGCT AACTGAACCA TCAGTTAGGA GTGTTCGCCT CACCTGGGTT
    CCAGGGGATG ATAACAACAG TCCAATTACA GGTTTCATTG TACAATTTGA GGAAGACAGG
    CATCAGCCCG GTACATGGCA CAACCTTGCC TCTGTCCCTG CTAATGTGAA CTCTGTCATA
    CTGCAGCTTT CTCCTTATGT CAACTACCAG TTTCGTGTTC TTGCACTAAA TGAACTTGGG
    AGCAGTATGC CAAGCCCATC TTCAGAACGT TTTCAGACAG CAGGAGCACC TCCAGAAACT
    AATCCAGGTG ATGTGAAGGG AGCAGGGACT AGCAAAAACA ATATGGAAAT CACATGGACA
    CCACTGAACT CCACTCAGGC GTATGGTCCA AACCTGCGCT ACAACGTGAA GTGGCGCCAA
    CGTCACACAG GAGAACCTTG GAGCAATATT ACAGTGTGGG ACAACAAGCA TGTGGTGTGG
    AATACACCAG TATACACCCA ATATGAGCTA AGAGTTCAGG CGGAAAATGA ATTTGGAAAG
    GCTCCAGAGC CAAGCACCAT GATTGGATAT TCTGGTGAAG ACTATCCTAA GGCTGCACCC
    ACTGACATTA AGCTTCGGGT CATGAACAGC ACCGCCATCT CTCTTCAGTG GACACGTGTG
    GCACCTGCCT CTATCCAAGG AAGCCTACTG GAATACAGAG CTTATTTTTG GAGAGAAAGT
    AGTTTGCTGA AGGGTGTCTG GGTCTCACGA AAGAAGCAAC AACAGAGTTT TATGGGGGAC
    CGTGTGAGAG GCATCGTATC TCAACTGATT CCATACAGCA TCTACAAGCT GTACTTGGTG
    GCAGTGAATG GCCGAGGGGA TGGCCCCAAA AGTGATGTTA AGGAATTCAC AACCCCAGAG
    GGAGTGCCGA GTGCTCCTAA ATACTTTAGA GTATATGAGC CAAACCGGGA AATGATTGTT
    GTTGAATGGG GTCACCCAGA GCACCCGAAC GGAAACTTGA CTGCATACAA CCTCCGCATC
    CAACCATTTA ATGGCACCAG AACAGGGAAG GCTGTATTTG AAACTCTTTC ACGTAATCAA
    ACCAAATATA CCCTGCAAAG AACAGATCCC GTGTCCCGGT ATCGGTTTGC TATCAATGCC
    AGAACGCAGG TTGGTGCAGG GGAAGTCATC ACAGCTGAGT CTCCTGTCAG ACTGGATGAA
    GTTTCTCCCA CGTCAGTGTC TTCTAAGTCT CCTGCACCTG TCACAGATGC TCCCACAGAG
    GTCCCAACTA TTCCCCCAAC CTCATCTGGT GCCACCACTC TTCTAGCTAC AACCAGTACA
    ACCAGTGTCA CAACGACTAC TGCTGGTACA GAAAGTTCGC TGGTCGCCAT TACTAGAATT
    CCTAATATAG CCACCACTGG ACCATCTCTA TGGAACCTAA CAGCATTACC CAACACTTAC
    TGGGCAAACA TCACCTGGAT GCATAACTTA GAGCCAGGCT CTGTGTTTAT GGTTGAGTAT
    ACTGACAGTA ATAATACAGT GAAAACTGTT CCTGTTAAGG CTCATGTCCC TTCTATCCAA
    CTGTTTGATC TGGTGCCTGG GATGACCTAC AGGGTGCGGG TTTACTCCCA GGAGCACCCG
    TCAGTCACAA GCCTATATGT GACATTTGAT ACCAGCTCAG CTTACACCAA GAACCAAGTT
    GACATTGCTA CACAAGGCTG GTTTATTGGG CTCATGTGTG CAGTTGCTCT ACTGGTACTG
    ATTTTGCTGA TCGTTTGTTT TATCAAAAGA AGCCGAGGAG GCAAATACCC AGTGCGAGAA
    AAGAAGGAGG TGGCGTTGGG ACCAGATGAT AACAAAGAGG GGGATGGCTC ATTTGACTAC
    AGTGATGAGG ACAACAAGCC CCTGCAGGGT AGTCAAACTT CGCTGGATGG AACCATAAAG
    CAACAAGAGA GTGATGACAG CCTTGTTGAT TATGGAGAAG GAGGAGAGGG GCAGTTTAAT
    GAAGATGGAT CGTTTATAGG GCAGTATACA GTGAAAAAGG ACAAAGATGA GACTGAGGGA
    AATGAAAGTT CAGAGGCCAC ATCTCCTGTA AATGCTATCT ATTCTCTGGC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa33491
    Clone ID Related Accession (Same CDS sequence) XM_031896831.1
    Accession Version XM_031896831.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4002bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neurofascin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGACAGATT TTCTAGAAAG GAGGTTAACG GTGATCCACT ACTACTGTCT CGGAAGCAAC 
    ATGCCATCAT GTTTAGTCCT TCTCCTGCTC TTTCTCCACC TAGTCTGCAC TATTAAAGTT
    CCACTGGACC CAAACATTCA GCGAGACCTA ATACAGCCCC CAACCATCAT CAAGCAGTCA
    GCAAAGAATT ATATTGTAGA TCCCCGTGAT AACATCCTAA TTGAGTGTGA AGCTCAGGGG
    AATCCTTACC CAATGTTTTC ATGGACAAAA AATGGGAAAT TTTACAATGT GGCAAAAGAC
    CCTCGAGTGT CATTCCGTCG ACGATCAGGC ACCCTTGAAA TAGAAATTCA TGGAGGAGGG
    CGGCCAGAGG ACTATGAGGG AGAATACCAA TGCTCTGCCC GCAACCAACA TGGAACAGCT
    TTATCAAATA AGATCTTACT TCAAGTCTCT CGATCCCCTT TGTGGCCCAA AGAAAACCTT
    GACCCGGTGG TGGTAAATGA AGGTGCCTTC TTGATCCTTC AGTGCAATCC CCCACATGGA
    CTTCCTCCTC CAGTCATATT TTGGATGAGC AGCTTGATGG AGCCTATTGT CCAGGACAAG
    AGGGTTTCTC AGGGACTGAA TGGGGACCTT TATTTCTCCA ACGTTCTGCG CCAGGATACT
    CAGACTGATT ATAGCTGCAA TGCACGCTCC CATTTCACAC ACACCATTCA ACAGAAGAAC
    CCATATACTC TAAAAGTGCT TACCACCAGA GGGGTACCTG AGAGAACCCC AACCTTCTTG
    CTTCCCACGG GGACTTCTAG CAGTAAACTG GTGTTACGCG ATGAGGACTT GCTACTGGAG
    TGTATTGCTT CTGGAGTACC AACGCCAGAC ATCATGTGGT ATAAGAAAGG AGGAGAGCTA
    CCCAGCAGGA AGCTGAAGTT TGAAAATTTT AACAAGACTC TACGCATAAT TGGAGTGTCT
    GAAGAGGACT CGGGGGAGTA TTTCTGCCAA GCTTCAAACA AAATGGGCAG CATTCGGCAC
    ACGATTTCTG TTCGGGTTAA GGCTGCTCCA TATTGGCTTG ATGAGCCCAA GAACTTGATT
    CTTGCACCAG GTGAGAACGG ACGTTTGGTA TGTCGTGCCA GTGGCAGCCC CAAACCTACA
    ATCCAATGGT TTATGAATGG AGATGCTACT GAAGTAGGTG CCCCAAATCC CAACTTCGAA
    ATCGCTGGTG ATACCATTAT TTTTAGAGAA GTACAGAGTG GCAACAGCGC TGTATACCAA
    TGCAATGCTT CTAATGAACA TGGTTATCTG TTAGCTAATG CCTTCGTCAG CATTCTGGAT
    GTTCCATCTC GGATGTTGGG CCCTCGTAAT CAGCTGATTC GAGTGATAGA ATACAACAGG
    ACCCAGCTAG ACTGCCCTTT CTTTGGCTCT CCTATACCTA CACTCCGCTG GTTTAAAAAT
    GGACAGGGTA GTAACCTCGA TGGTGGCAAT TACCGGGTGC ATGAAAATGG GACCCTTGAG
    ATTAATACTG TGAGGAAGGA GGACCAAGGC AAATACACAT GTGTTGCTAC CAATATTTTG
    GGCAATGCTG AAAATACTGT GCGGCTGGAG GTCAAAGAGC CAACTCGTAT TGTAAAGGGC
    CCTGAAGATC AGATTGCGAA GAGGGGCTCT TCTGTGCATC TGGACTGTCG AGTGCGCAGT
    GACTCCTCAC TCAGACTCAC TGTTACCTGG ATAAAAGATG ACACTCAGTT GTATATAAAC
    AGCAGAATGA AGAAGGATGA GGATGGACTT ACAATATTCG GTGTGGCAGA AAAGGATCAG
    GGAGCATATA CATGTGTTGC TAAAACTGAA TTAGACCAGG ACTCTTCTAA AGCTTATCTC
    ACGATACAGG AAACTTCCTC ACCTACTAGC CGACTGACCT CCCAGCTTAT AGACAAACCA
    GACCCCCCAC AGGACCTGGA GCTAACTGAA CCATCAGTTA GGAGTGTTCG CCTCACCTGG
    GTTCCAGGGG ATGATAACAA CAGTCCAATT ACAGGTTTCA TTGTACAATT TGAGGAAGAC
    AGGCATCAGC CCGGTACATG GCACAACCTT GCCTCTGTCC CTGCTAATGT GAACTCTGTC
    ATACTGCAGC TTTCTCCTTA TGTCAACTAC CAGTTTCGTG TTCTTGCACT AAATGAACTT
    GGGAGCAGTA TGCCAAGCCC ATCTTCAGAA CGTTTTCAGA CAGCAGGAGC ACCTCCAGAA
    ACTAATCCAG GTGATGTGAA GGGAGCAGGG ACTAGCAAAA ACAATATGGA AATCACATGG
    ACACCACTGA ACTCCACTCA GGCGTATGGT CCAAACCTGC GCTACAACGT GAAGTGGCGC
    CAACGTCACA CAGGAGAACC TTGGAGCAAT ATTACAGTGT GGGACAACAA GCATGTGGTG
    TGGAATACAC CAGTATACAC CCAATATGAG CTAAGAGTTC AGGCGGAAAA TGAATTTGGA
    AAGGCTCCAG AGCCAAGCAC CATGATTGGA TATTCTGGTG AAGACTATCC TAAGGCTGCA
    CCCACTGACA TTAAGCTTCG GGTCATGAAC AGCACCGCCA TCTCTCTTCA GTGGACACGT
    GTGGCACCTG CCTCTATCCA AGGAAGCCTA CTGGAATACA GAGCTTATTT TTGGAGAGAA
    AGTAGTTTGC TGAAGGGTGT CTGGGTCTCA CGAAAGAAGC AACAACAGAG TTTTATGGGG
    GACCGTGTGA GAGGCATCGT ATCTCAACTG ATTCCATACA GCATCTACAA GCTGTACTTG
    GTGGCAGTGA ATGGCCGAGG GGATGGCCCC AAAAGTGATG TTAAGGAATT CACAACCCCA
    GAGGGAGTGC CGAGTGCTCC TAAATACTTT AGAGTATATG AGCCAAACCG GGAAATGATT
    GTTGTTGAAT GGGGTCACCC AGAGCACCCG AACGGAAACT TGACTGCATA CAACCTCCGC
    ATCCAACCAT TTAATGGCAC CAGAACAGGG AAGGCTGTAT TTGAAACTCT TTCACGTAAT
    CAAACCAAAT ATACCCTGCA AAGAACAGAT CCCGTGTCCC GGTATCGGTT TGCTATCAAT
    GCCAGAACGC AGGTTGGTGC AGGGGAAGTC ATCACAGCTG AGTCTCCTGT CAGACTGGAT
    GAAGTGTCTT CTAAGTCTCC TGCACCTGTC ACAGATGCTC CCACAGAGGT CCCAACTATT
    CCCCCAACCT CATCTGGTGC CACCACTCTT CTAGCTACAA CCAGTACAAC CAGTGTCACA
    ACGACTACTG CTGGTACAGA AAGTTCGCTG GTCGCCATTA CTAGAATTCC TAATATAGCC
    ACCACTGGAC CATCTCTATG GAACCTAACA GCATTACCCA ACACTTACTG GGCAAACATC
    ACCTGGATGC ATAACTTAGA GCCAGGCTCT GTGTTTATGG TTGAGTATAC TGACAGTAAT
    AATACAGTGA AAACTGTTCC TGTTAAGGCT CATGTCCCTT CTATCCAACT GTTTGATCTG
    GTGCCTGGGA TGACCTACAG GGTGCGGGTT TACTCCCAGG AGCACCCGTC AGTCACAAGC
    CTATATGTGA CATTTGATAC CAGCTCAGCT TACACCAAGA ACCAAGTTGA CATTGCTACA
    CAAGGCTGGT TTATTGGGCT CATGTGTGCA GTTGCTCTAC TGGTACTGAT TTTGCTGATC
    GTTTGTTTTA TCAAAAGAAG CCGAGGAGGC AAATACCCAG TGCGAGAAAA GAAGGAGGTG
    GCGTTGGGAC CAGATGATAA CAAAGAGGGG GATGGCTCAT TTGACTACAG TGATGAGGAC
    AACAAGCCCC TGCAGGGTAG TCAAACTTCG CTGGATGGAA CCATAAAGCA ACAAGAGAGT
    GATGACAGCC TTGTTGATTA TGGAGAAGGA GGAGAGGGGC AGTTTAATGA AGATGGATCG
    TTTATAGGGC AGTATACAGT GAAAAAGGAC AAAGATGAGA CTGAGGGAAA TGAAAGTTCA
    GAGGCCACAT CTCCTGTAAA TGCTATCTAT TCTCTGGCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031752691.1
    CDS547..4548
    Translation

    Target ORF information:

    RefSeq Version XM_031896831.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neurofascin (nfasc), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896831.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGACAGATT TTCTAGAAAG GAGGTTAACG GTGATCCACT ACTACTGTCT CGGAAGCAAC 
    ATGCCATCAT GTTTAGTCCT TCTCCTGCTC TTTCTCCACC TAGTCTGCAC TATTAAAGTT
    CCACTGGACC CAAACATTCA GCGAGACCTA ATACAGCCCC CAACCATCAT CAAGCAGTCA
    GCAAAGAATT ATATTGTAGA TCCCCGTGAT AACATCCTAA TTGAGTGTGA AGCTCAGGGG
    AATCCTTACC CAATGTTTTC ATGGACAAAA AATGGGAAAT TTTACAATGT GGCAAAAGAC
    CCTCGAGTGT CATTCCGTCG ACGATCAGGC ACCCTTGAAA TAGAAATTCA TGGAGGAGGG
    CGGCCAGAGG ACTATGAGGG AGAATACCAA TGCTCTGCCC GCAACCAACA TGGAACAGCT
    TTATCAAATA AGATCTTACT TCAAGTCTCT CGATCCCCTT TGTGGCCCAA AGAAAACCTT
    GACCCGGTGG TGGTAAATGA AGGTGCCTTC TTGATCCTTC AGTGCAATCC CCCACATGGA
    CTTCCTCCTC CAGTCATATT TTGGATGAGC AGCTTGATGG AGCCTATTGT CCAGGACAAG
    AGGGTTTCTC AGGGACTGAA TGGGGACCTT TATTTCTCCA ACGTTCTGCG CCAGGATACT
    CAGACTGATT ATAGCTGCAA TGCACGCTCC CATTTCACAC ACACCATTCA ACAGAAGAAC
    CCATATACTC TAAAAGTGCT TACCACCAGA GGGGTACCTG AGAGAACCCC AACCTTCTTG
    CTTCCCACGG GGACTTCTAG CAGTAAACTG GTGTTACGCG ATGAGGACTT GCTACTGGAG
    TGTATTGCTT CTGGAGTACC AACGCCAGAC ATCATGTGGT ATAAGAAAGG AGGAGAGCTA
    CCCAGCAGGA AGCTGAAGTT TGAAAATTTT AACAAGACTC TACGCATAAT TGGAGTGTCT
    GAAGAGGACT CGGGGGAGTA TTTCTGCCAA GCTTCAAACA AAATGGGCAG CATTCGGCAC
    ACGATTTCTG TTCGGGTTAA GGCTGCTCCA TATTGGCTTG ATGAGCCCAA GAACTTGATT
    CTTGCACCAG GTGAGAACGG ACGTTTGGTA TGTCGTGCCA GTGGCAGCCC CAAACCTACA
    ATCCAATGGT TTATGAATGG AGATGCTACT GAAGTAGGTG CCCCAAATCC CAACTTCGAA
    ATCGCTGGTG ATACCATTAT TTTTAGAGAA GTACAGAGTG GCAACAGCGC TGTATACCAA
    TGCAATGCTT CTAATGAACA TGGTTATCTG TTAGCTAATG CCTTCGTCAG CATTCTGGAT
    GTTCCATCTC GGATGTTGGG CCCTCGTAAT CAGCTGATTC GAGTGATAGA ATACAACAGG
    ACCCAGCTAG ACTGCCCTTT CTTTGGCTCT CCTATACCTA CACTCCGCTG GTTTAAAAAT
    GGACAGGGTA GTAACCTCGA TGGTGGCAAT TACCGGGTGC ATGAAAATGG GACCCTTGAG
    ATTAATACTG TGAGGAAGGA GGACCAAGGC AAATACACAT GTGTTGCTAC CAATATTTTG
    GGCAATGCTG AAAATACTGT GCGGCTGGAG GTCAAAGAGC CAACTCGTAT TGTAAAGGGC
    CCTGAAGATC AGATTGCGAA GAGGGGCTCT TCTGTGCATC TGGACTGTCG AGTGCGCAGT
    GACTCCTCAC TCAGACTCAC TGTTACCTGG ATAAAAGATG ACACTCAGTT GTATATAAAC
    AGCAGAATGA AGAAGGATGA GGATGGACTT ACAATATTCG GTGTGGCAGA AAAGGATCAG
    GGAGCATATA CATGTGTTGC TAAAACTGAA TTAGACCAGG ACTCTTCTAA AGCTTATCTC
    ACGATACAGG AAACTTCCTC ACCTACTAGC CGACTGACCT CCCAGCTTAT AGACAAACCA
    GACCCCCCAC AGGACCTGGA GCTAACTGAA CCATCAGTTA GGAGTGTTCG CCTCACCTGG
    GTTCCAGGGG ATGATAACAA CAGTCCAATT ACAGGTTTCA TTGTACAATT TGAGGAAGAC
    AGGCATCAGC CCGGTACATG GCACAACCTT GCCTCTGTCC CTGCTAATGT GAACTCTGTC
    ATACTGCAGC TTTCTCCTTA TGTCAACTAC CAGTTTCGTG TTCTTGCACT AAATGAACTT
    GGGAGCAGTA TGCCAAGCCC ATCTTCAGAA CGTTTTCAGA CAGCAGGAGC ACCTCCAGAA
    ACTAATCCAG GTGATGTGAA GGGAGCAGGG ACTAGCAAAA ACAATATGGA AATCACATGG
    ACACCACTGA ACTCCACTCA GGCGTATGGT CCAAACCTGC GCTACAACGT GAAGTGGCGC
    CAACGTCACA CAGGAGAACC TTGGAGCAAT ATTACAGTGT GGGACAACAA GCATGTGGTG
    TGGAATACAC CAGTATACAC CCAATATGAG CTAAGAGTTC AGGCGGAAAA TGAATTTGGA
    AAGGCTCCAG AGCCAAGCAC CATGATTGGA TATTCTGGTG AAGACTATCC TAAGGCTGCA
    CCCACTGACA TTAAGCTTCG GGTCATGAAC AGCACCGCCA TCTCTCTTCA GTGGACACGT
    GTGGCACCTG CCTCTATCCA AGGAAGCCTA CTGGAATACA GAGCTTATTT TTGGAGAGAA
    AGTAGTTTGC TGAAGGGTGT CTGGGTCTCA CGAAAGAAGC AACAACAGAG TTTTATGGGG
    GACCGTGTGA GAGGCATCGT ATCTCAACTG ATTCCATACA GCATCTACAA GCTGTACTTG
    GTGGCAGTGA ATGGCCGAGG GGATGGCCCC AAAAGTGATG TTAAGGAATT CACAACCCCA
    GAGGGAGTGC CGAGTGCTCC TAAATACTTT AGAGTATATG AGCCAAACCG GGAAATGATT
    GTTGTTGAAT GGGGTCACCC AGAGCACCCG AACGGAAACT TGACTGCATA CAACCTCCGC
    ATCCAACCAT TTAATGGCAC CAGAACAGGG AAGGCTGTAT TTGAAACTCT TTCACGTAAT
    CAAACCAAAT ATACCCTGCA AAGAACAGAT CCCGTGTCCC GGTATCGGTT TGCTATCAAT
    GCCAGAACGC AGGTTGGTGC AGGGGAAGTC ATCACAGCTG AGTCTCCTGT CAGACTGGAT
    GAAGTGTCTT CTAAGTCTCC TGCACCTGTC ACAGATGCTC CCACAGAGGT CCCAACTATT
    CCCCCAACCT CATCTGGTGC CACCACTCTT CTAGCTACAA CCAGTACAAC CAGTGTCACA
    ACGACTACTG CTGGTACAGA AAGTTCGCTG GTCGCCATTA CTAGAATTCC TAATATAGCC
    ACCACTGGAC CATCTCTATG GAACCTAACA GCATTACCCA ACACTTACTG GGCAAACATC
    ACCTGGATGC ATAACTTAGA GCCAGGCTCT GTGTTTATGG TTGAGTATAC TGACAGTAAT
    AATACAGTGA AAACTGTTCC TGTTAAGGCT CATGTCCCTT CTATCCAACT GTTTGATCTG
    GTGCCTGGGA TGACCTACAG GGTGCGGGTT TACTCCCAGG AGCACCCGTC AGTCACAAGC
    CTATATGTGA CATTTGATAC CAGCTCAGCT TACACCAAGA ACCAAGTTGA CATTGCTACA
    CAAGGCTGGT TTATTGGGCT CATGTGTGCA GTTGCTCTAC TGGTACTGAT TTTGCTGATC
    GTTTGTTTTA TCAAAAGAAG CCGAGGAGGC AAATACCCAG TGCGAGAAAA GAAGGAGGTG
    GCGTTGGGAC CAGATGATAA CAAAGAGGGG GATGGCTCAT TTGACTACAG TGATGAGGAC
    AACAAGCCCC TGCAGGGTAG TCAAACTTCG CTGGATGGAA CCATAAAGCA ACAAGAGAGT
    GATGACAGCC TTGTTGATTA TGGAGAAGGA GGAGAGGGGC AGTTTAATGA AGATGGATCG
    TTTATAGGGC AGTATACAGT GAAAAAGGAC AAAGATGAGA CTGAGGGAAA TGAAAGTTCA
    GAGGCCACAT CTCCTGTAAA TGCTATCTAT TCTCTGGCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa33493
    Clone ID Related Accession (Same CDS sequence) XM_031896835.1
    Accession Version XM_031896835.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3573bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neurofascin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGACAGATT TTCTAGAAAG GAGGTTAACG GTGATCCACT ACTACTGTCT CGGAAGCAAC 
    ATGCCATCAT GTTTAGTCCT TCTCCTGCTC TTTCTCCACC TAGTCTGCAC TATTAAAGTT
    CCACTGGACC CAAACATTCA GCGAGACCTA ATACAGCCCC CAACCATCAT CAAGCAGTCA
    GCAAAGAATT ATATTGTAGA TCCCCGTGAT AACATCCTAA TTGAGTGTGA AGCTCAGGGG
    AATCCTTACC CAATGTTTTC ATGGACAAAA AATGGGAAAT TTTACAATGT GGCAAAAGAC
    CCTCGAGTGT CATTCCGTCG ACGATCAGGC ACCCTTGAAA TAGAAATTCA TGGAGGAGGG
    CGGCCAGAGG ACTATGAGGG AGAATACCAA TGCTCTGCCC GCAACCAACA TGGAACAGCT
    TTATCAAATA AGATCTTACT TCAAGTCTCT CGATCCCCTT TGTGGCCCAA AGAAAACCTT
    GACCCGGTGG TGGTAAATGA AGGTGCCTTC TTGATCCTTC AGTGCAATCC CCCACATGGA
    CTTCCTCCTC CAGTCATATT TTGGATGAGC AGCTTGATGG AGCCTATTGT CCAGGACAAG
    AGGGTTTCTC AGGGACTGAA TGGGGACCTT TATTTCTCCA ACGTTCTGCG CCAGGATACT
    CAGACTGATT ATAGCTGCAA TGCACGCTCC CATTTCACAC ACACCATTCA ACAGAAGAAC
    CCATATACTC TAAAAGTGCT TACCACCAGA GGGGTACCTG AGAGAACCCC AACCTTCTTG
    CTTCCCACGG GGACTTCTAG CAGTAAACTG GTGTTACGCG ATGAGGACTT GCTACTGGAG
    TGTATTGCTT CTGGAGTACC AACGCCAGAC ATCATGTGGT ATAAGAAAGG AGGAGAGCTA
    CCCAGCAGGA AGCTGAAGTT TGAAAATTTT AACAAGACTC TACGCATAAT TGGAGTGTCT
    GAAGAGGACT CGGGGGAGTA TTTCTGCCAA GCTTCAAACA AAATGGGCAG CATTCGGCAC
    ACGATTTCTG TTCGGGTTAA GGCTGCTCCA TATTGGCTTG ATGAGCCCAA GAACTTGATT
    CTTGCACCAG GTGAGAACGG ACGTTTGGTA TGTCGTGCCA GTGGCAGCCC CAAACCTACA
    ATCCAATGGT TTATGAATGG AGATGCTACT GAAGTAGGTG CCCCAAATCC CAACTTCGAA
    ATCGCTGGTG ATACCATTAT TTTTAGAGAA GTACAGAGTG GCAACAGCGC TGTATACCAA
    TGCAATGCTT CTAATGAACA TGGTTATCTG TTAGCTAATG CCTTCGTCAG CATTCTGGAT
    GTTCCATCTC GGATGTTGGG CCCTCGTAAT CAGCTGATTC GAGTGATAGA ATACAACAGG
    ACCCAGCTAG ACTGCCCTTT CTTTGGCTCT CCTATACCTA CACTCCGCTG GTTTAAAAAT
    GGACAGGGTA GTAACCTCGA TGGTGGCAAT TACCGGGTGC ATGAAAATGG GACCCTTGAG
    ATTAATACTG TGAGGAAGGA GGACCAAGGC AAATACACAT GTGTTGCTAC CAATATTTTG
    GGCAATGCTG AAAATACTGT GCGGCTGGAG GTCAAAGAGC CAACTCGTAT TGTAAAGGGC
    CCTGAAGATC AGATTGCGAA GAGGGGCTCT TCTGTGCATC TGGACTGTCG AGTGCGCAGT
    GACTCCTCAC TCAGACTCAC TGTTACCTGG ATAAAAGATG ACACTCAGTT GTATATAAAC
    AGCAGAATGA AGAAGGATGA GGATGGACTT ACAATATTCG GTGTGGCAGA AAAGGATCAG
    GGAGCATATA CATGTGTTGC TAAAACTGAA TTAGACCAGG ACTCTTCTAA AGCTTATCTC
    ACGATACAGG AAACTTCCTC ACCTACTAGC CGACTGACCT CCCAGCTTAT AGACAAACCA
    GACCCCCCAC AGGACCTGGA GCTAACTGAA CCATCAGTTA GGAGTGTTCG CCTCACCTGG
    GTTCCAGGGG ATGATAACAA CAGTCCAATT ACAGGTTTCA TTGTACAATT TGAGGAAGAC
    AGGCATCAGC CCGGTACATG GCACAACCTT GCCTCTGTCC CTGCTAATGT GAACTCTGTC
    ATACTGCAGC TTTCTCCTTA TGTCAACTAC CAGTTTCGTG TTCTTGCACT AAATGAACTT
    GGGAGCAGTA TGCCAAGCCC ATCTTCAGAA CGTTTTCAGA CAGCAGGAGC ACCTCCAGAA
    ACTAATCCAG GTGATGTGAA GGGAGCAGGG ACTAGCAAAA ACAATATGGA AATCACATGG
    ACACCACTGA ACTCCACTCA GGCGTATGGT CCAAACCTGC GCTACAACGT GAAGTGGCGC
    CAACGTCACA CAGGAGAACC TTGGAGCAAT ATTACAGTGT GGGACAACAA GCATGTGGTG
    TGGAATACAC CAGTATACAC CCAATATGAG CTAAGAGTTC AGGCGGAAAA TGAATTTGGA
    AAGGCTCCAG AGCCAAGCAC CATGATTGGA TATTCTGGTG AAGACTATCC TAAGGCTGCA
    CCCACTGACA TTAAGCTTCG GGTCATGAAC AGCACCGCCA TCTCTCTTCA GTGGACACGT
    GTGGCACCTG CCTCTATCCA AGGAAGCCTA CTGGAATACA GAGCTTATTT TTGGAGAGAA
    AGTAGTTTGC TGAAGGGTGT CTGGGTCTCA CGAAAGAAGC AACAACAGAG TTTTATGGGG
    GACCGTGTGA GAGGCATCGT ATCTCAACTG ATTCCATACA GCATCTACAA GCTGTACTTG
    GTGGCAGTGA ATGGCCGAGG GGATGGCCCC AAAAGTGATG TTAAGGAATT CACAACCCCA
    GAGGGAGTGC CGAGTGCTCC TAAATACTTT AGAGTATATG AGCCAAACCG GGAAATGATT
    GTTGTTGAAT GGGGTCACCC AGAGCACCCG AACGGAAACT TGACTGCATA CAACCTCCGC
    ATCCAACCAT TTAATGGCAC CAGAACAGGG AAGGCTGTAT TTGAAACTCT TTCACGTAAT
    CAAACCAAAT ATACCCTGCA AAGAACAGAT CCCGTGTCCC GGTATCGGTT TGCTATCAAT
    GCCAGAACGC AGGTTGGTGC AGGGGAAGTC ATCACAGCTG AGTCTCCTGT CAGACTGGAT
    GAAGTTTCTC CCACGTCAGC TTACACCAAG AACCAAGTTG ACATTGCTAC ACAAGGCTGG
    TTTATTGGGC TCATGTGTGC AGTTGCTCTA CTGGTACTGA TTTTGCTGAT CGTTTGTTTT
    ATCAAAAGAA GCCGAGGAGG CAAATACCCA GTGCGAGAAA AGAAGGAGGT GGCGTTGGGA
    CCAGATGATA ACAAAGAGGG GGATGGCTCA TTTGACTACA GTGATGAGGA CAACAAGCCC
    CTGCAGGGTA GTCAAACTTC GCTGGATGGA ACCATAAAGC AACAAGAGAG TGATGACAGC
    CTTGTTGATT ATGGAGAAGG AGGAGAGGGG CAGTTTAATG AAGATGGATC GTTTATAGGG
    CAGTATACAG TGAAAAAGGA CAAAGATGAG ACTGAGGGAA ATGAAAGTTC AGAGGCCACA
    TCTCCTGTAA ATGCTATCTA TTCTCTGGCA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031752695.1
    CDS546..4118
    Translation

    Target ORF information:

    RefSeq Version XM_031896835.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neurofascin (nfasc), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896835.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGACAGATT TTCTAGAAAG GAGGTTAACG GTGATCCACT ACTACTGTCT CGGAAGCAAC 
    ATGCCATCAT GTTTAGTCCT TCTCCTGCTC TTTCTCCACC TAGTCTGCAC TATTAAAGTT
    CCACTGGACC CAAACATTCA GCGAGACCTA ATACAGCCCC CAACCATCAT CAAGCAGTCA
    GCAAAGAATT ATATTGTAGA TCCCCGTGAT AACATCCTAA TTGAGTGTGA AGCTCAGGGG
    AATCCTTACC CAATGTTTTC ATGGACAAAA AATGGGAAAT TTTACAATGT GGCAAAAGAC
    CCTCGAGTGT CATTCCGTCG ACGATCAGGC ACCCTTGAAA TAGAAATTCA TGGAGGAGGG
    CGGCCAGAGG ACTATGAGGG AGAATACCAA TGCTCTGCCC GCAACCAACA TGGAACAGCT
    TTATCAAATA AGATCTTACT TCAAGTCTCT CGATCCCCTT TGTGGCCCAA AGAAAACCTT
    GACCCGGTGG TGGTAAATGA AGGTGCCTTC TTGATCCTTC AGTGCAATCC CCCACATGGA
    CTTCCTCCTC CAGTCATATT TTGGATGAGC AGCTTGATGG AGCCTATTGT CCAGGACAAG
    AGGGTTTCTC AGGGACTGAA TGGGGACCTT TATTTCTCCA ACGTTCTGCG CCAGGATACT
    CAGACTGATT ATAGCTGCAA TGCACGCTCC CATTTCACAC ACACCATTCA ACAGAAGAAC
    CCATATACTC TAAAAGTGCT TACCACCAGA GGGGTACCTG AGAGAACCCC AACCTTCTTG
    CTTCCCACGG GGACTTCTAG CAGTAAACTG GTGTTACGCG ATGAGGACTT GCTACTGGAG
    TGTATTGCTT CTGGAGTACC AACGCCAGAC ATCATGTGGT ATAAGAAAGG AGGAGAGCTA
    CCCAGCAGGA AGCTGAAGTT TGAAAATTTT AACAAGACTC TACGCATAAT TGGAGTGTCT
    GAAGAGGACT CGGGGGAGTA TTTCTGCCAA GCTTCAAACA AAATGGGCAG CATTCGGCAC
    ACGATTTCTG TTCGGGTTAA GGCTGCTCCA TATTGGCTTG ATGAGCCCAA GAACTTGATT
    CTTGCACCAG GTGAGAACGG ACGTTTGGTA TGTCGTGCCA GTGGCAGCCC CAAACCTACA
    ATCCAATGGT TTATGAATGG AGATGCTACT GAAGTAGGTG CCCCAAATCC CAACTTCGAA
    ATCGCTGGTG ATACCATTAT TTTTAGAGAA GTACAGAGTG GCAACAGCGC TGTATACCAA
    TGCAATGCTT CTAATGAACA TGGTTATCTG TTAGCTAATG CCTTCGTCAG CATTCTGGAT
    GTTCCATCTC GGATGTTGGG CCCTCGTAAT CAGCTGATTC GAGTGATAGA ATACAACAGG
    ACCCAGCTAG ACTGCCCTTT CTTTGGCTCT CCTATACCTA CACTCCGCTG GTTTAAAAAT
    GGACAGGGTA GTAACCTCGA TGGTGGCAAT TACCGGGTGC ATGAAAATGG GACCCTTGAG
    ATTAATACTG TGAGGAAGGA GGACCAAGGC AAATACACAT GTGTTGCTAC CAATATTTTG
    GGCAATGCTG AAAATACTGT GCGGCTGGAG GTCAAAGAGC CAACTCGTAT TGTAAAGGGC
    CCTGAAGATC AGATTGCGAA GAGGGGCTCT TCTGTGCATC TGGACTGTCG AGTGCGCAGT
    GACTCCTCAC TCAGACTCAC TGTTACCTGG ATAAAAGATG ACACTCAGTT GTATATAAAC
    AGCAGAATGA AGAAGGATGA GGATGGACTT ACAATATTCG GTGTGGCAGA AAAGGATCAG
    GGAGCATATA CATGTGTTGC TAAAACTGAA TTAGACCAGG ACTCTTCTAA AGCTTATCTC
    ACGATACAGG AAACTTCCTC ACCTACTAGC CGACTGACCT CCCAGCTTAT AGACAAACCA
    GACCCCCCAC AGGACCTGGA GCTAACTGAA CCATCAGTTA GGAGTGTTCG CCTCACCTGG
    GTTCCAGGGG ATGATAACAA CAGTCCAATT ACAGGTTTCA TTGTACAATT TGAGGAAGAC
    AGGCATCAGC CCGGTACATG GCACAACCTT GCCTCTGTCC CTGCTAATGT GAACTCTGTC
    ATACTGCAGC TTTCTCCTTA TGTCAACTAC CAGTTTCGTG TTCTTGCACT AAATGAACTT
    GGGAGCAGTA TGCCAAGCCC ATCTTCAGAA CGTTTTCAGA CAGCAGGAGC ACCTCCAGAA
    ACTAATCCAG GTGATGTGAA GGGAGCAGGG ACTAGCAAAA ACAATATGGA AATCACATGG
    ACACCACTGA ACTCCACTCA GGCGTATGGT CCAAACCTGC GCTACAACGT GAAGTGGCGC
    CAACGTCACA CAGGAGAACC TTGGAGCAAT ATTACAGTGT GGGACAACAA GCATGTGGTG
    TGGAATACAC CAGTATACAC CCAATATGAG CTAAGAGTTC AGGCGGAAAA TGAATTTGGA
    AAGGCTCCAG AGCCAAGCAC CATGATTGGA TATTCTGGTG AAGACTATCC TAAGGCTGCA
    CCCACTGACA TTAAGCTTCG GGTCATGAAC AGCACCGCCA TCTCTCTTCA GTGGACACGT
    GTGGCACCTG CCTCTATCCA AGGAAGCCTA CTGGAATACA GAGCTTATTT TTGGAGAGAA
    AGTAGTTTGC TGAAGGGTGT CTGGGTCTCA CGAAAGAAGC AACAACAGAG TTTTATGGGG
    GACCGTGTGA GAGGCATCGT ATCTCAACTG ATTCCATACA GCATCTACAA GCTGTACTTG
    GTGGCAGTGA ATGGCCGAGG GGATGGCCCC AAAAGTGATG TTAAGGAATT CACAACCCCA
    GAGGGAGTGC CGAGTGCTCC TAAATACTTT AGAGTATATG AGCCAAACCG GGAAATGATT
    GTTGTTGAAT GGGGTCACCC AGAGCACCCG AACGGAAACT TGACTGCATA CAACCTCCGC
    ATCCAACCAT TTAATGGCAC CAGAACAGGG AAGGCTGTAT TTGAAACTCT TTCACGTAAT
    CAAACCAAAT ATACCCTGCA AAGAACAGAT CCCGTGTCCC GGTATCGGTT TGCTATCAAT
    GCCAGAACGC AGGTTGGTGC AGGGGAAGTC ATCACAGCTG AGTCTCCTGT CAGACTGGAT
    GAAGTTTCTC CCACGTCAGC TTACACCAAG AACCAAGTTG ACATTGCTAC ACAAGGCTGG
    TTTATTGGGC TCATGTGTGC AGTTGCTCTA CTGGTACTGA TTTTGCTGAT CGTTTGTTTT
    ATCAAAAGAA GCCGAGGAGG CAAATACCCA GTGCGAGAAA AGAAGGAGGT GGCGTTGGGA
    CCAGATGATA ACAAAGAGGG GGATGGCTCA TTTGACTACA GTGATGAGGA CAACAAGCCC
    CTGCAGGGTA GTCAAACTTC GCTGGATGGA ACCATAAAGC AACAAGAGAG TGATGACAGC
    CTTGTTGATT ATGGAGAAGG AGGAGAGGGG CAGTTTAATG AAGATGGATC GTTTATAGGG
    CAGTATACAG TGAAAAAGGA CAAAGATGAG ACTGAGGGAA ATGAAAGTTC AGAGGCCACA
    TCTCCTGTAA ATGCTATCTA TTCTCTGGCA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52226
    Clone ID Related Accession (Same CDS sequence) XM_031896832.1
    Accession Version XM_031896832.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3975bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neurofascin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGACAGATT TTCTAGAAAG GAGGTTAACG GTGATCCACT ACTACTGTCT CGGAAGCAAC 
    ATGCCATCAT GTTTAGTCCT TCTCCTGCTC TTTCTCCACC TAGTCTGCAC TATTAAAGTT
    CCACTGGACC CAAACATTCA GCGAGACCTA ATACAGCCCC CAACCATCAT CAAGCAGTCA
    GCAAAGAATT ATATTGTAGA TCCCCGTGAT AACATCCTAA TTGAGTGTGA AGCTCAGGGG
    AATCCTTACC CAATGTTTTC ATGGACAAAA AATGGGAAAT TTTACAATGT GGCAAAAGAC
    CCTCGAGTGT CATTCCGTCG ACGATCAGGC ACCCTTGAAA TAGAAATTCA TGGAGGAGGG
    CGGCCAGAGG ACTATGAGGG AGAATACCAA TGCTCTGCCC GCAACCAACA TGGAACAGCT
    TTATCAAATA AGATCTTACT TCAAGTCTCT CGATCCCCTT TGTGGCCCAA AGAAAACCTT
    GACCCGGTGG TGGTAAATGA AGGTGCCTTC TTGATCCTTC AGTGCAATCC CCCACATGGA
    CTTCCTCCTC CAGTCATATT TTGGATGAGC AGCTTGATGG AGCCTATTGT CCAGGACAAG
    AGGGTTTCTC AGGGACTGAA TGGGGACCTT TATTTCTCCA ACGTTCTGCG CCAGGATACT
    CAGACTGATT ATAGCTGCAA TGCACGCTCC CATTTCACAC ACACCATTCA ACAGAAGAAC
    CCATATACTC TAAAAGTGCT TACCACCAGA GGGGTACCTG AGAGAACCCC AACCTTCTTG
    CTTCCCACGG GGACTTCTAG CAGTAAACTG GTGTTACGCG ATGAGGACTT GCTACTGGAG
    TGTATTGCTT CTGGAGTACC AACGCCAGAC ATCATGTGGT ATAAGAAAGG AGGAGAGCTA
    CCCAGCAGGA AGCTGAAGTT TGAAAATTTT AACAAGACTC TACGCATAAT TGGAGTGTCT
    GAAGAGGACT CGGGGGAGTA TTTCTGCCAA GCTTCAAACA AAATGGGCAG CATTCGGCAC
    ACGATTTCTG TTCGGGTTAA GGCTGCTCCA TATTGGCTTG ATGAGCCCAA GAACTTGATT
    CTTGCACCAG GTGAGAACGG ACGTTTGGTA TGTCGTGCCA GTGGCAGCCC CAAACCTACA
    ATCCAATGGT TTATGAATGG AGATGCTACT GAAGTAGGTG CCCCAAATCC CAACTTCGAA
    ATCGCTGGTG ATACCATTAT TTTTAGAGAA GTACAGAGTG GCAACAGCGC TGTATACCAA
    TGCAATGCTT CTAATGAACA TGGTTATCTG TTAGCTAATG CCTTCGTCAG CATTCTGGAT
    GTTCCATCTC GGATGTTGGG CCCTCGTAAT CAGCTGATTC GAGTGATAGA ATACAACAGG
    ACCCAGCTAG ACTGCCCTTT CTTTGGCTCT CCTATACCTA CACTCCGCTG GTTTAAAAAT
    GGACAGGGTA GTAACCTCGA TGGTGGCAAT TACCGGGTGC ATGAAAATGG GACCCTTGAG
    ATTAATACTG TGAGGAAGGA GGACCAAGGC AAATACACAT GTGTTGCTAC CAATATTTTG
    GGCAATGCTG AAAATACTGT GCGGCTGGAG GTCAAAGAGC CAACTCGTAT TGTAAAGGGC
    CCTGAAGATC AGATTGCGAA GAGGGGCTCT TCTGTGCATC TGGACTGTCG AGTGCGCAGT
    GACTCCTCAC TCAGACTCAC TGTTACCTGG ATAAAAGATG ACACTCAGTT GTATATAAAC
    AGCAGAATGA AGAAGGATGA GGATGGACTT ACAATATTCG GTGTGGCAGA AAAGGATCAG
    GGAGCATATA CATGTGTTGC TAAAACTGAA TTAGACCAGG ACTCTTCTAA AGCTTATCTC
    ACGATACAGG ACAAACCAGA CCCCCCACAG GACCTGGAGC TAACTGAACC ATCAGTTAGG
    AGTGTTCGCC TCACCTGGGT TCCAGGGGAT GATAACAACA GTCCAATTAC AGGTTTCATT
    GTACAATTTG AGGAAGACAG GCATCAGCCC GGTACATGGC ACAACCTTGC CTCTGTCCCT
    GCTAATGTGA ACTCTGTCAT ACTGCAGCTT TCTCCTTATG TCAACTACCA GTTTCGTGTT
    CTTGCACTAA ATGAACTTGG GAGCAGTATG CCAAGCCCAT CTTCAGAACG TTTTCAGACA
    GCAGGAGCAC CTCCAGAAAC TAATCCAGGT GATGTGAAGG GAGCAGGGAC TAGCAAAAAC
    AATATGGAAA TCACATGGAC ACCACTGAAC TCCACTCAGG CGTATGGTCC AAACCTGCGC
    TACAACGTGA AGTGGCGCCA ACGTCACACA GGAGAACCTT GGAGCAATAT TACAGTGTGG
    GACAACAAGC ATGTGGTGTG GAATACACCA GTATACACCC AATATGAGCT AAGAGTTCAG
    GCGGAAAATG AATTTGGAAA GGCTCCAGAG CCAAGCACCA TGATTGGATA TTCTGGTGAA
    GACTATCCTA AGGCTGCACC CACTGACATT AAGCTTCGGG TCATGAACAG CACCGCCATC
    TCTCTTCAGT GGACACGTGT GGCACCTGCC TCTATCCAAG GAAGCCTACT GGAATACAGA
    GCTTATTTTT GGAGAGAAAG TAGTTTGCTG AAGGGTGTCT GGGTCTCACG AAAGAAGCAA
    CAACAGAGTT TTATGGGGGA CCGTGTGAGA GGCATCGTAT CTCAACTGAT TCCATACAGC
    ATCTACAAGC TGTACTTGGT GGCAGTGAAT GGCCGAGGGG ATGGCCCCAA AAGTGATGTT
    AAGGAATTCA CAACCCCAGA GGGAGTGCCG AGTGCTCCTA AATACTTTAG AGTATATGAG
    CCAAACCGGG AAATGATTGT TGTTGAATGG GGTCACCCAG AGCACCCGAA CGGAAACTTG
    ACTGCATACA ACCTCCGCAT CCAACCATTT AATGGCACCA GAACAGGGAA GGCTGTATTT
    GAAACTCTTT CACGTAATCA AACCAAATAT ACCCTGCAAA GAACAGATCC CGTGTCCCGG
    TATCGGTTTG CTATCAATGC CAGAACGCAG GTTGGTGCAG GGGAAGTCAT CACAGCTGAG
    TCTCCTGTCA GACTGGATGA AGTTTCTCCC ACGTCAGTGT CTTCTAAGTC TCCTGCACCT
    GTCACAGATG CTCCCACAGA GGTCCCAACT ATTCCCCCAA CCTCATCTGG TGCCACCACT
    CTTCTAGCTA CAACCAGTAC AACCAGTGTC ACAACGACTA CTGCTGGTAC AGAAAGTTCG
    CTGGTCGCCA TTACTAGAAT TCCTAATATA GCCACCACTG GACCATCTCT ATGGAACCTA
    ACAGCATTAC CCAACACTTA CTGGGCAAAC ATCACCTGGA TGCATAACTT AGAGCCAGGC
    TCTGTGTTTA TGGTTGAGTA TACTGACAGT AATAATACAG TGAAAACTGT TCCTGTTAAG
    GCTCATGTCC CTTCTATCCA ACTGTTTGAT CTGGTGCCTG GGATGACCTA CAGGGTGCGG
    GTTTACTCCC AGGAGCACCC GTCAGTCACA AGCCTATATG TGACATTTGA TACCAGCTCA
    GCTTACACCA AGAACCAAGT TGACATTGCT ACACAAGGCT GGTTTATTGG GCTCATGTGT
    GCAGTTGCTC TACTGGTACT GATTTTGCTG ATCGTTTGTT TTATCAAAAG AAGCCGAGGA
    GGCAAATACC CAGTGCGAGA AAAGAAGGAG GTGGCGTTGG GACCAGATGA TAACAAAGAG
    GGGGATGGCT CATTTGACTA CAGTGATGAG GACAACAAGC CCCTGCAGGG TAGTCAAACT
    TCGCTGGATG GAACCATAAA GCAACAAGAG AGTGATGACA GCCTTGTTGA TTATGGAGAA
    GGAGGAGAGG GGCAGTTTAA TGAAGATGGA TCGTTTATAG GGCAGTATAC AGTGAAAAAG
    GACAAAGATG AGACTGAGGG AAATGAAAGT TCAGAGGCCA CATCTCCTGT AAATGCTATC
    TATTCTCTGG CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031752692.1
    CDS547..4521
    Translation

    Target ORF information:

    RefSeq Version XM_031896832.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neurofascin (nfasc), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896832.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGACAGATT TTCTAGAAAG GAGGTTAACG GTGATCCACT ACTACTGTCT CGGAAGCAAC 
    ATGCCATCAT GTTTAGTCCT TCTCCTGCTC TTTCTCCACC TAGTCTGCAC TATTAAAGTT
    CCACTGGACC CAAACATTCA GCGAGACCTA ATACAGCCCC CAACCATCAT CAAGCAGTCA
    GCAAAGAATT ATATTGTAGA TCCCCGTGAT AACATCCTAA TTGAGTGTGA AGCTCAGGGG
    AATCCTTACC CAATGTTTTC ATGGACAAAA AATGGGAAAT TTTACAATGT GGCAAAAGAC
    CCTCGAGTGT CATTCCGTCG ACGATCAGGC ACCCTTGAAA TAGAAATTCA TGGAGGAGGG
    CGGCCAGAGG ACTATGAGGG AGAATACCAA TGCTCTGCCC GCAACCAACA TGGAACAGCT
    TTATCAAATA AGATCTTACT TCAAGTCTCT CGATCCCCTT TGTGGCCCAA AGAAAACCTT
    GACCCGGTGG TGGTAAATGA AGGTGCCTTC TTGATCCTTC AGTGCAATCC CCCACATGGA
    CTTCCTCCTC CAGTCATATT TTGGATGAGC AGCTTGATGG AGCCTATTGT CCAGGACAAG
    AGGGTTTCTC AGGGACTGAA TGGGGACCTT TATTTCTCCA ACGTTCTGCG CCAGGATACT
    CAGACTGATT ATAGCTGCAA TGCACGCTCC CATTTCACAC ACACCATTCA ACAGAAGAAC
    CCATATACTC TAAAAGTGCT TACCACCAGA GGGGTACCTG AGAGAACCCC AACCTTCTTG
    CTTCCCACGG GGACTTCTAG CAGTAAACTG GTGTTACGCG ATGAGGACTT GCTACTGGAG
    TGTATTGCTT CTGGAGTACC AACGCCAGAC ATCATGTGGT ATAAGAAAGG AGGAGAGCTA
    CCCAGCAGGA AGCTGAAGTT TGAAAATTTT AACAAGACTC TACGCATAAT TGGAGTGTCT
    GAAGAGGACT CGGGGGAGTA TTTCTGCCAA GCTTCAAACA AAATGGGCAG CATTCGGCAC
    ACGATTTCTG TTCGGGTTAA GGCTGCTCCA TATTGGCTTG ATGAGCCCAA GAACTTGATT
    CTTGCACCAG GTGAGAACGG ACGTTTGGTA TGTCGTGCCA GTGGCAGCCC CAAACCTACA
    ATCCAATGGT TTATGAATGG AGATGCTACT GAAGTAGGTG CCCCAAATCC CAACTTCGAA
    ATCGCTGGTG ATACCATTAT TTTTAGAGAA GTACAGAGTG GCAACAGCGC TGTATACCAA
    TGCAATGCTT CTAATGAACA TGGTTATCTG TTAGCTAATG CCTTCGTCAG CATTCTGGAT
    GTTCCATCTC GGATGTTGGG CCCTCGTAAT CAGCTGATTC GAGTGATAGA ATACAACAGG
    ACCCAGCTAG ACTGCCCTTT CTTTGGCTCT CCTATACCTA CACTCCGCTG GTTTAAAAAT
    GGACAGGGTA GTAACCTCGA TGGTGGCAAT TACCGGGTGC ATGAAAATGG GACCCTTGAG
    ATTAATACTG TGAGGAAGGA GGACCAAGGC AAATACACAT GTGTTGCTAC CAATATTTTG
    GGCAATGCTG AAAATACTGT GCGGCTGGAG GTCAAAGAGC CAACTCGTAT TGTAAAGGGC
    CCTGAAGATC AGATTGCGAA GAGGGGCTCT TCTGTGCATC TGGACTGTCG AGTGCGCAGT
    GACTCCTCAC TCAGACTCAC TGTTACCTGG ATAAAAGATG ACACTCAGTT GTATATAAAC
    AGCAGAATGA AGAAGGATGA GGATGGACTT ACAATATTCG GTGTGGCAGA AAAGGATCAG
    GGAGCATATA CATGTGTTGC TAAAACTGAA TTAGACCAGG ACTCTTCTAA AGCTTATCTC
    ACGATACAGG ACAAACCAGA CCCCCCACAG GACCTGGAGC TAACTGAACC ATCAGTTAGG
    AGTGTTCGCC TCACCTGGGT TCCAGGGGAT GATAACAACA GTCCAATTAC AGGTTTCATT
    GTACAATTTG AGGAAGACAG GCATCAGCCC GGTACATGGC ACAACCTTGC CTCTGTCCCT
    GCTAATGTGA ACTCTGTCAT ACTGCAGCTT TCTCCTTATG TCAACTACCA GTTTCGTGTT
    CTTGCACTAA ATGAACTTGG GAGCAGTATG CCAAGCCCAT CTTCAGAACG TTTTCAGACA
    GCAGGAGCAC CTCCAGAAAC TAATCCAGGT GATGTGAAGG GAGCAGGGAC TAGCAAAAAC
    AATATGGAAA TCACATGGAC ACCACTGAAC TCCACTCAGG CGTATGGTCC AAACCTGCGC
    TACAACGTGA AGTGGCGCCA ACGTCACACA GGAGAACCTT GGAGCAATAT TACAGTGTGG
    GACAACAAGC ATGTGGTGTG GAATACACCA GTATACACCC AATATGAGCT AAGAGTTCAG
    GCGGAAAATG AATTTGGAAA GGCTCCAGAG CCAAGCACCA TGATTGGATA TTCTGGTGAA
    GACTATCCTA AGGCTGCACC CACTGACATT AAGCTTCGGG TCATGAACAG CACCGCCATC
    TCTCTTCAGT GGACACGTGT GGCACCTGCC TCTATCCAAG GAAGCCTACT GGAATACAGA
    GCTTATTTTT GGAGAGAAAG TAGTTTGCTG AAGGGTGTCT GGGTCTCACG AAAGAAGCAA
    CAACAGAGTT TTATGGGGGA CCGTGTGAGA GGCATCGTAT CTCAACTGAT TCCATACAGC
    ATCTACAAGC TGTACTTGGT GGCAGTGAAT GGCCGAGGGG ATGGCCCCAA AAGTGATGTT
    AAGGAATTCA CAACCCCAGA GGGAGTGCCG AGTGCTCCTA AATACTTTAG AGTATATGAG
    CCAAACCGGG AAATGATTGT TGTTGAATGG GGTCACCCAG AGCACCCGAA CGGAAACTTG
    ACTGCATACA ACCTCCGCAT CCAACCATTT AATGGCACCA GAACAGGGAA GGCTGTATTT
    GAAACTCTTT CACGTAATCA AACCAAATAT ACCCTGCAAA GAACAGATCC CGTGTCCCGG
    TATCGGTTTG CTATCAATGC CAGAACGCAG GTTGGTGCAG GGGAAGTCAT CACAGCTGAG
    TCTCCTGTCA GACTGGATGA AGTTTCTCCC ACGTCAGTGT CTTCTAAGTC TCCTGCACCT
    GTCACAGATG CTCCCACAGA GGTCCCAACT ATTCCCCCAA CCTCATCTGG TGCCACCACT
    CTTCTAGCTA CAACCAGTAC AACCAGTGTC ACAACGACTA CTGCTGGTAC AGAAAGTTCG
    CTGGTCGCCA TTACTAGAAT TCCTAATATA GCCACCACTG GACCATCTCT ATGGAACCTA
    ACAGCATTAC CCAACACTTA CTGGGCAAAC ATCACCTGGA TGCATAACTT AGAGCCAGGC
    TCTGTGTTTA TGGTTGAGTA TACTGACAGT AATAATACAG TGAAAACTGT TCCTGTTAAG
    GCTCATGTCC CTTCTATCCA ACTGTTTGAT CTGGTGCCTG GGATGACCTA CAGGGTGCGG
    GTTTACTCCC AGGAGCACCC GTCAGTCACA AGCCTATATG TGACATTTGA TACCAGCTCA
    GCTTACACCA AGAACCAAGT TGACATTGCT ACACAAGGCT GGTTTATTGG GCTCATGTGT
    GCAGTTGCTC TACTGGTACT GATTTTGCTG ATCGTTTGTT TTATCAAAAG AAGCCGAGGA
    GGCAAATACC CAGTGCGAGA AAAGAAGGAG GTGGCGTTGG GACCAGATGA TAACAAAGAG
    GGGGATGGCT CATTTGACTA CAGTGATGAG GACAACAAGC CCCTGCAGGG TAGTCAAACT
    TCGCTGGATG GAACCATAAA GCAACAAGAG AGTGATGACA GCCTTGTTGA TTATGGAGAA
    GGAGGAGAGG GGCAGTTTAA TGAAGATGGA TCGTTTATAG GGCAGTATAC AGTGAAAAAG
    GACAAAGATG AGACTGAGGG AAATGAAAGT TCAGAGGCCA CATCTCCTGT AAATGCTATC
    TATTCTCTGG CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52227
    Clone ID Related Accession (Same CDS sequence) XM_031896833.1
    Accession Version XM_031896833.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3972bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neurofascin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGACAGATT TTCTAGAAAG GAGGTTAACG GTGATCCACT ACTACTGTCT CGGAAGCAAC 
    ATGCCATCAT GTTTAGTCCT TCTCCTGCTC TTTCTCCACC TAGTCTGCAC TATTAAAGTT
    CCACTGGACC CAAACATTCA GCGAGACCTA ATACAGCCCC CAACCATCAT CAAGCAGTCA
    GCAAAGAATT ATATTGTAGA TCCCCGTGAT AACATCCTAA TTGAGTGTGA AGCTCAGGGG
    AATCCTTACC CAATGTTTTC ATGGACAAAA AATGGGAAAT TTTACAATGT GGCAAAAGAC
    CCTCGAGTGT CATTCCGTCG ACGATCAGGC ACCCTTGAAA TAGAAATTCA TGGAGGAGGG
    CGGCCAGAGG ACTATGAGGG AGAATACCAA TGCTCTGCCC GCAACCAACA TGGAACAGCT
    TTATCAAATA AGATCTTACT TCAAGTCTCT CGATCCCCTT TGTGGCCCAA AGAAAACCTT
    GACCCGGTGG TGGTAAATGA AGGTGCCTTC TTGATCCTTC AGTGCAATCC CCCACATGGA
    CTTCCTCCTC CAGTCATATT TTGGATGAGC AGCTTGATGG AGCCTATTGT CCAGGACAAG
    AGGGTTTCTC AGGGACTGAA TGGGGACCTT TATTTCTCCA ACGTTCTGCG CCAGGATACT
    CAGACTGATT ATAGCTGCAA TGCACGCTCC CATTTCACAC ACACCATTCA ACAGAAGAAC
    CCATATACTC TAAAAGTGCT TACCACCAGA GGGGTACCTG AGAGAACCCC AACCTTCTTG
    CTTCCCACGG GGACTTCTAG CAGTAAACTG GTGTTACGCG ATGAGGACTT GCTACTGGAG
    TGTATTGCTT CTGGAGTACC AACGCCAGAC ATCATGTGGT ATAAGAAAGG AGGAGAGCTA
    CCCAGCAGGA AGCTGAAGTT TGAAAATTTT AACAAGACTC TACGCATAAT TGGAGTGTCT
    GAAGAGGACT CGGGGGAGTA TTTCTGCCAA GCTTCAAACA AAATGGGCAG CATTCGGCAC
    ACGATTTCTG TTCGGGTTAA GGCTGCTCCA TATTGGCTTG ATGAGCCCAA GAACTTGATT
    CTTGCACCAG GTGAGAACGG ACGTTTGGTA TGTCGTGCCA GTGGCAGCCC CAAACCTACA
    ATCCAATGGT TTATGAATGG AGATGCTACT GAAGTAGGTG CCCCAAATCC CAACTTCGAA
    ATCGCTGGTG ATACCATTAT TTTTAGAGAA GTACAGAGTG GCAACAGCGC TGTATACCAA
    TGCAATGCTT CTAATGAACA TGGTTATCTG TTAGCTAATG CCTTCGTCAG CATTCTGGAT
    GTTCCATCTC GGATGTTGGG CCCTCGTAAT CAGCTGATTC GAGTGATAGA ATACAACAGG
    ACCCAGCTAG ACTGCCCTTT CTTTGGCTCT CCTATACCTA CACTCCGCTG GTTTAAAAAT
    GGACAGGGTA GTAACCTCGA TGGTGGCAAT TACCGGGTGC ATGAAAATGG GACCCTTGAG
    ATTAATACTG TGAGGAAGGA GGACCAAGGC AAATACACAT GTGTTGCTAC CAATATTTTG
    GGCAATGCTG AAAATACTGT GCGGCTGGAG GTCAAAGAGC CAACTCGTAT TGTAAAGGGC
    CCTGAAGATC AGATTGCGAA GAGGGGCTCT TCTGTGCATC TGGACTGTCG AGTGCGCAGT
    GACTCCTCAC TCAGACTCAC TGTTACCTGG ATAAAAGATG ACACTCAGTT GTATATAAAC
    AGCAGAATGA AGAAGGATGA GGATGGACTT ACAATATTCG GTGTGGCAGA AAAGGATCAG
    GGAGCATATA CATGTGTTGC TAAAACTGAA TTAGACCAGG ACTCTTCTAA AGCTTATCTC
    ACGATACAGG AAACTTCCTC ACCTACTAGC CGACTGACCT CCCAGCTTAT AGACAAACCA
    GACCCCCCAC AGGACCTGGA GCTAACTGAA CCATCAGTTA GGAGTGTTCG CCTCACCTGG
    GTTCCAGGGG ATGATAACAA CAGTCCAATT ACAGGTTTCA TTGTACAATT TGAGGAAGAC
    AGGCATCAGC CCGGTACATG GCACAACCTT GCCTCTGTCC CTGCTAATGT GAACTCTGTC
    ATACTGCAGC TTTCTCCTTA TGTCAACTAC CAGTTTCGTG TTCTTGCACT AAATGAACTT
    GGGAGCAGTA TGCCAAGCCC ATCTTCAGAA CGTTTTCAGA CAGCAGGAGC ACCTCCAGAA
    ACTAATCCAG GTGATGTGAA GGGAGCAGGG ACTAGCAAAA ACAATATGGA AATCACATGG
    ACACCACTGA ACTCCACTCA GGCGTATGGT CCAAACCTGC GCTACAACGT GAAGTGGCGC
    CAACGTCACA CAGGAGAACC TTGGAGCAAT ATTACAGTGT GGGACAACAA GCATGTGGTG
    TGGAATACAC CAGTATACAC CCAATATGAG CTAAGAGTTC AGGCGGAAAA TGAATTTGGA
    AAGGCTCCAG AGCCAAGCAC CATGATTGGA TATTCTGGTG AAGACTATCC TAAGGCTGCA
    CCCACTGACA TTAAGCTTCG GGTCATGAAC AGCACCGCCA TCTCTCTTCA GTGGACACGT
    GTGGCACCTG CCTCTATCCA AGGAAGCCTA CTGGAATACA GAGCTTATTT TTGGAGAGAA
    AGTAGTTTGC TGAAGGGTGT CTGGGTCTCA CGAAAGAAGC AACAACAGAG TTTTATGGGG
    GACCGTGTGA GAGGCATCGT ATCTCAACTG ATTCCATACA GCATCTACAA GCTGTACTTG
    GTGGCAGTGA ATGGCCGAGG GGATGGCCCC AAAAGTGATG TTAAGGAATT CACAACCCCA
    GAGGGAGTGC CGAGTGCTCC TAAATACTTT AGAGTATATG AGCCAAACCG GGAAATGATT
    GTTGTTGAAT GGGGTCACCC AGAGCACCCG AACGGAAACT TGACTGCATA CAACCTCCGC
    ATCCAACCAT TTAATGGCAC CAGAACAGGG AAGGCTGTAT TTGAAACTCT TTCACGTAAT
    CAAACCAAAT ATACCCTGCA AAGAACAGAT CCCGTGTCCC GGTATCGGTT TGCTATCAAT
    GCCAGAACGC AGGTTGGTGC AGGGGAAGTC ATCACAGCTG AGTCTCCTGT CAGACTGGAT
    GAAGTTTCTC CCACGTCAGT GTCTTCTAAG TCTCCTGCAC CTGTCACAGA TGCTCCCACA
    GAGGTCCCAA CTATTCCCCC AACCTCATCT GGTGCCACCA CTCTTCTAGC TACAACCAGT
    ACAACCAGTG TCACAACGAC TACTGCTGCC ACCACTGGAC CATCTCTATG GAACCTAACA
    GCATTACCCA ACACTTACTG GGCAAACATC ACCTGGATGC ATAACTTAGA GCCAGGCTCT
    GTGTTTATGG TTGAGTATAC TGACAGTAAT AATACAGTGA AAACTGTTCC TGTTAAGGCT
    CATGTCCCTT CTATCCAACT GTTTGATCTG GTGCCTGGGA TGACCTACAG GGTGCGGGTT
    TACTCCCAGG AGCACCCGTC AGTCACAAGC CTATATGTGA CATTTGATAC CAGCTCAGCT
    TACACCAAGA ACCAAGTTGA CATTGCTACA CAAGGCTGGT TTATTGGGCT CATGTGTGCA
    GTTGCTCTAC TGGTACTGAT TTTGCTGATC GTTTGTTTTA TCAAAAGAAG CCGAGGAGGC
    AAATACCCAG TGCGAGAAAA GAAGGAGGTG GCGTTGGGAC CAGATGATAA CAAAGAGGGG
    GATGGCTCAT TTGACTACAG TGATGAGGAC AACAAGCCCC TGCAGGGTAG TCAAACTTCG
    CTGGATGGAA CCATAAAGCA ACAAGAGAGT GATGACAGCC TTGTTGATTA TGGAGAAGGA
    GGAGAGGGGC AGTTTAATGA AGATGGATCG TTTATAGGGC AGTATACAGT GAAAAAGGAC
    AAAGATGAGA CTGAGGGAAA TGAAAGTTCA GAGGCCACAT CTCCTGTAAA TGCTATCTAT
    TCTCTGGCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031752693.1
    CDS547..4518
    Translation

    Target ORF information:

    RefSeq Version XM_031896833.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neurofascin (nfasc), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896833.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGACAGATT TTCTAGAAAG GAGGTTAACG GTGATCCACT ACTACTGTCT CGGAAGCAAC 
    ATGCCATCAT GTTTAGTCCT TCTCCTGCTC TTTCTCCACC TAGTCTGCAC TATTAAAGTT
    CCACTGGACC CAAACATTCA GCGAGACCTA ATACAGCCCC CAACCATCAT CAAGCAGTCA
    GCAAAGAATT ATATTGTAGA TCCCCGTGAT AACATCCTAA TTGAGTGTGA AGCTCAGGGG
    AATCCTTACC CAATGTTTTC ATGGACAAAA AATGGGAAAT TTTACAATGT GGCAAAAGAC
    CCTCGAGTGT CATTCCGTCG ACGATCAGGC ACCCTTGAAA TAGAAATTCA TGGAGGAGGG
    CGGCCAGAGG ACTATGAGGG AGAATACCAA TGCTCTGCCC GCAACCAACA TGGAACAGCT
    TTATCAAATA AGATCTTACT TCAAGTCTCT CGATCCCCTT TGTGGCCCAA AGAAAACCTT
    GACCCGGTGG TGGTAAATGA AGGTGCCTTC TTGATCCTTC AGTGCAATCC CCCACATGGA
    CTTCCTCCTC CAGTCATATT TTGGATGAGC AGCTTGATGG AGCCTATTGT CCAGGACAAG
    AGGGTTTCTC AGGGACTGAA TGGGGACCTT TATTTCTCCA ACGTTCTGCG CCAGGATACT
    CAGACTGATT ATAGCTGCAA TGCACGCTCC CATTTCACAC ACACCATTCA ACAGAAGAAC
    CCATATACTC TAAAAGTGCT TACCACCAGA GGGGTACCTG AGAGAACCCC AACCTTCTTG
    CTTCCCACGG GGACTTCTAG CAGTAAACTG GTGTTACGCG ATGAGGACTT GCTACTGGAG
    TGTATTGCTT CTGGAGTACC AACGCCAGAC ATCATGTGGT ATAAGAAAGG AGGAGAGCTA
    CCCAGCAGGA AGCTGAAGTT TGAAAATTTT AACAAGACTC TACGCATAAT TGGAGTGTCT
    GAAGAGGACT CGGGGGAGTA TTTCTGCCAA GCTTCAAACA AAATGGGCAG CATTCGGCAC
    ACGATTTCTG TTCGGGTTAA GGCTGCTCCA TATTGGCTTG ATGAGCCCAA GAACTTGATT
    CTTGCACCAG GTGAGAACGG ACGTTTGGTA TGTCGTGCCA GTGGCAGCCC CAAACCTACA
    ATCCAATGGT TTATGAATGG AGATGCTACT GAAGTAGGTG CCCCAAATCC CAACTTCGAA
    ATCGCTGGTG ATACCATTAT TTTTAGAGAA GTACAGAGTG GCAACAGCGC TGTATACCAA
    TGCAATGCTT CTAATGAACA TGGTTATCTG TTAGCTAATG CCTTCGTCAG CATTCTGGAT
    GTTCCATCTC GGATGTTGGG CCCTCGTAAT CAGCTGATTC GAGTGATAGA ATACAACAGG
    ACCCAGCTAG ACTGCCCTTT CTTTGGCTCT CCTATACCTA CACTCCGCTG GTTTAAAAAT
    GGACAGGGTA GTAACCTCGA TGGTGGCAAT TACCGGGTGC ATGAAAATGG GACCCTTGAG
    ATTAATACTG TGAGGAAGGA GGACCAAGGC AAATACACAT GTGTTGCTAC CAATATTTTG
    GGCAATGCTG AAAATACTGT GCGGCTGGAG GTCAAAGAGC CAACTCGTAT TGTAAAGGGC
    CCTGAAGATC AGATTGCGAA GAGGGGCTCT TCTGTGCATC TGGACTGTCG AGTGCGCAGT
    GACTCCTCAC TCAGACTCAC TGTTACCTGG ATAAAAGATG ACACTCAGTT GTATATAAAC
    AGCAGAATGA AGAAGGATGA GGATGGACTT ACAATATTCG GTGTGGCAGA AAAGGATCAG
    GGAGCATATA CATGTGTTGC TAAAACTGAA TTAGACCAGG ACTCTTCTAA AGCTTATCTC
    ACGATACAGG AAACTTCCTC ACCTACTAGC CGACTGACCT CCCAGCTTAT AGACAAACCA
    GACCCCCCAC AGGACCTGGA GCTAACTGAA CCATCAGTTA GGAGTGTTCG CCTCACCTGG
    GTTCCAGGGG ATGATAACAA CAGTCCAATT ACAGGTTTCA TTGTACAATT TGAGGAAGAC
    AGGCATCAGC CCGGTACATG GCACAACCTT GCCTCTGTCC CTGCTAATGT GAACTCTGTC
    ATACTGCAGC TTTCTCCTTA TGTCAACTAC CAGTTTCGTG TTCTTGCACT AAATGAACTT
    GGGAGCAGTA TGCCAAGCCC ATCTTCAGAA CGTTTTCAGA CAGCAGGAGC ACCTCCAGAA
    ACTAATCCAG GTGATGTGAA GGGAGCAGGG ACTAGCAAAA ACAATATGGA AATCACATGG
    ACACCACTGA ACTCCACTCA GGCGTATGGT CCAAACCTGC GCTACAACGT GAAGTGGCGC
    CAACGTCACA CAGGAGAACC TTGGAGCAAT ATTACAGTGT GGGACAACAA GCATGTGGTG
    TGGAATACAC CAGTATACAC CCAATATGAG CTAAGAGTTC AGGCGGAAAA TGAATTTGGA
    AAGGCTCCAG AGCCAAGCAC CATGATTGGA TATTCTGGTG AAGACTATCC TAAGGCTGCA
    CCCACTGACA TTAAGCTTCG GGTCATGAAC AGCACCGCCA TCTCTCTTCA GTGGACACGT
    GTGGCACCTG CCTCTATCCA AGGAAGCCTA CTGGAATACA GAGCTTATTT TTGGAGAGAA
    AGTAGTTTGC TGAAGGGTGT CTGGGTCTCA CGAAAGAAGC AACAACAGAG TTTTATGGGG
    GACCGTGTGA GAGGCATCGT ATCTCAACTG ATTCCATACA GCATCTACAA GCTGTACTTG
    GTGGCAGTGA ATGGCCGAGG GGATGGCCCC AAAAGTGATG TTAAGGAATT CACAACCCCA
    GAGGGAGTGC CGAGTGCTCC TAAATACTTT AGAGTATATG AGCCAAACCG GGAAATGATT
    GTTGTTGAAT GGGGTCACCC AGAGCACCCG AACGGAAACT TGACTGCATA CAACCTCCGC
    ATCCAACCAT TTAATGGCAC CAGAACAGGG AAGGCTGTAT TTGAAACTCT TTCACGTAAT
    CAAACCAAAT ATACCCTGCA AAGAACAGAT CCCGTGTCCC GGTATCGGTT TGCTATCAAT
    GCCAGAACGC AGGTTGGTGC AGGGGAAGTC ATCACAGCTG AGTCTCCTGT CAGACTGGAT
    GAAGTTTCTC CCACGTCAGT GTCTTCTAAG TCTCCTGCAC CTGTCACAGA TGCTCCCACA
    GAGGTCCCAA CTATTCCCCC AACCTCATCT GGTGCCACCA CTCTTCTAGC TACAACCAGT
    ACAACCAGTG TCACAACGAC TACTGCTGCC ACCACTGGAC CATCTCTATG GAACCTAACA
    GCATTACCCA ACACTTACTG GGCAAACATC ACCTGGATGC ATAACTTAGA GCCAGGCTCT
    GTGTTTATGG TTGAGTATAC TGACAGTAAT AATACAGTGA AAACTGTTCC TGTTAAGGCT
    CATGTCCCTT CTATCCAACT GTTTGATCTG GTGCCTGGGA TGACCTACAG GGTGCGGGTT
    TACTCCCAGG AGCACCCGTC AGTCACAAGC CTATATGTGA CATTTGATAC CAGCTCAGCT
    TACACCAAGA ACCAAGTTGA CATTGCTACA CAAGGCTGGT TTATTGGGCT CATGTGTGCA
    GTTGCTCTAC TGGTACTGAT TTTGCTGATC GTTTGTTTTA TCAAAAGAAG CCGAGGAGGC
    AAATACCCAG TGCGAGAAAA GAAGGAGGTG GCGTTGGGAC CAGATGATAA CAAAGAGGGG
    GATGGCTCAT TTGACTACAG TGATGAGGAC AACAAGCCCC TGCAGGGTAG TCAAACTTCG
    CTGGATGGAA CCATAAAGCA ACAAGAGAGT GATGACAGCC TTGTTGATTA TGGAGAAGGA
    GGAGAGGGGC AGTTTAATGA AGATGGATCG TTTATAGGGC AGTATACAGT GAAAAAGGAC
    AAAGATGAGA CTGAGGGAAA TGAAAGTTCA GAGGCCACAT CTCCTGTAAA TGCTATCTAT
    TCTCTGGCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52228
    Clone ID Related Accession (Same CDS sequence) XM_031896837.1
    Accession Version XM_031896837.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3531bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neurofascin isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGACAGATT TTCTAGAAAG GAGGTTAACG GTGATCCACT ACTACTGTCT CGGAAGCAAC 
    ATGCCATCAT GTTTAGTCCT TCTCCTGCTC TTTCTCCACC TAGTCTGCAC TATTAAAGTT
    CCACTGGACC CAAACATTCA GCGAGACCTA ATACAGCCCC CAACCATCAT CAAGCAGTCA
    GCAAAGAATT ATATTGTAGA TCCCCGTGAT AACATCCTAA TTGAGTGTGA AGCTCAGGGG
    AATCCTTACC CAATGTTTTC ATGGACAAAA AATGGGAAAT TTTACAATGT GGCAAAAGAC
    CCTCGAGTGT CATTCCGTCG ACGATCAGGC ACCCTTGAAA TAGAAATTCA TGGAGGAGGG
    CGGCCAGAGG ACTATGAGGG AGAATACCAA TGCTCTGCCC GCAACCAACA TGGAACAGCT
    TTATCAAATA AGATCTTACT TCAAGTCTCT CGATCCCCTT TGTGGCCCAA AGAAAACCTT
    GACCCGGTGG TGGTAAATGA AGGTGCCTTC TTGATCCTTC AGTGCAATCC CCCACATGGA
    CTTCCTCCTC CAGTCATATT TTGGATGAGC AGCTTGATGG AGCCTATTGT CCAGGACAAG
    AGGGTTTCTC AGGGACTGAA TGGGGACCTT TATTTCTCCA ACGTTCTGCG CCAGGATACT
    CAGACTGATT ATAGCTGCAA TGCACGCTCC CATTTCACAC ACACCATTCA ACAGAAGAAC
    CCATATACTC TAAAAGTGCT TACCACCAGA GGGGTACCTG AGAGAACCCC AACCTTCTTG
    CTTCCCACGG GGACTTCTAG CAGTAAACTG GTGTTACGCG ATGAGGACTT GCTACTGGAG
    TGTATTGCTT CTGGAGTACC AACGCCAGAC ATCATGTGGT ATAAGAAAGG AGGAGAGCTA
    CCCAGCAGGA AGCTGAAGTT TGAAAATTTT AACAAGACTC TACGCATAAT TGGAGTGTCT
    GAAGAGGACT CGGGGGAGTA TTTCTGCCAA GCTTCAAACA AAATGGGCAG CATTCGGCAC
    ACGATTTCTG TTCGGGTTAA GGCTGCTCCA TATTGGCTTG ATGAGCCCAA GAACTTGATT
    CTTGCACCAG GTGAGAACGG ACGTTTGGTA TGTCGTGCCA GTGGCAGCCC CAAACCTACA
    ATCCAATGGT TTATGAATGG AGATGCTACT GAAGTAGGTG CCCCAAATCC CAACTTCGAA
    ATCGCTGGTG ATACCATTAT TTTTAGAGAA GTACAGAGTG GCAACAGCGC TGTATACCAA
    TGCAATGCTT CTAATGAACA TGGTTATCTG TTAGCTAATG CCTTCGTCAG CATTCTGGAT
    GTTCCATCTC GGATGTTGGG CCCTCGTAAT CAGCTGATTC GAGTGATAGA ATACAACAGG
    ACCCAGCTAG ACTGCCCTTT CTTTGGCTCT CCTATACCTA CACTCCGCTG GTTTAAAAAT
    GGACAGGGTA GTAACCTCGA TGGTGGCAAT TACCGGGTGC ATGAAAATGG GACCCTTGAG
    ATTAATACTG TGAGGAAGGA GGACCAAGGC AAATACACAT GTGTTGCTAC CAATATTTTG
    GGCAATGCTG AAAATACTGT GCGGCTGGAG GTCAAAGAGC CAACTCGTAT TGTAAAGGGC
    CCTGAAGATC AGATTGCGAA GAGGGGCTCT TCTGTGCATC TGGACTGTCG AGTGCGCAGT
    GACTCCTCAC TCAGACTCAC TGTTACCTGG ATAAAAGATG ACACTCAGTT GTATATAAAC
    AGCAGAATGA AGAAGGATGA GGATGGACTT ACAATATTCG GTGTGGCAGA AAAGGATCAG
    GGAGCATATA CATGTGTTGC TAAAACTGAA TTAGACCAGG ACTCTTCTAA AGCTTATCTC
    ACGATACAGG ACAAACCAGA CCCCCCACAG GACCTGGAGC TAACTGAACC ATCAGTTAGG
    AGTGTTCGCC TCACCTGGGT TCCAGGGGAT GATAACAACA GTCCAATTAC AGGTTTCATT
    GTACAATTTG AGGAAGACAG GCATCAGCCC GGTACATGGC ACAACCTTGC CTCTGTCCCT
    GCTAATGTGA ACTCTGTCAT ACTGCAGCTT TCTCCTTATG TCAACTACCA GTTTCGTGTT
    CTTGCACTAA ATGAACTTGG GAGCAGTATG CCAAGCCCAT CTTCAGAACG TTTTCAGACA
    GCAGGAGCAC CTCCAGAAAC TAATCCAGGT GATGTGAAGG GAGCAGGGAC TAGCAAAAAC
    AATATGGAAA TCACATGGAC ACCACTGAAC TCCACTCAGG CGTATGGTCC AAACCTGCGC
    TACAACGTGA AGTGGCGCCA ACGTCACACA GGAGAACCTT GGAGCAATAT TACAGTGTGG
    GACAACAAGC ATGTGGTGTG GAATACACCA GTATACACCC AATATGAGCT AAGAGTTCAG
    GCGGAAAATG AATTTGGAAA GGCTCCAGAG CCAAGCACCA TGATTGGATA TTCTGGTGAA
    GACTATCCTA AGGCTGCACC CACTGACATT AAGCTTCGGG TCATGAACAG CACCGCCATC
    TCTCTTCAGT GGACACGTGT GGCACCTGCC TCTATCCAAG GAAGCCTACT GGAATACAGA
    GCTTATTTTT GGAGAGAAAG TAGTTTGCTG AAGGGTGTCT GGGTCTCACG AAAGAAGCAA
    CAACAGAGTT TTATGGGGGA CCGTGTGAGA GGCATCGTAT CTCAACTGAT TCCATACAGC
    ATCTACAAGC TGTACTTGGT GGCAGTGAAT GGCCGAGGGG ATGGCCCCAA AAGTGATGTT
    AAGGAATTCA CAACCCCAGA GGGAGTGCCG AGTGCTCCTA AATACTTTAG AGTATATGAG
    CCAAACCGGG AAATGATTGT TGTTGAATGG GGTCACCCAG AGCACCCGAA CGGAAACTTG
    ACTGCATACA ACCTCCGCAT CCAACCATTT AATGGCACCA GAACAGGGAA GGCTGTATTT
    GAAACTCTTT CACGTAATCA AACCAAATAT ACCCTGCAAA GAACAGATCC CGTGTCCCGG
    TATCGGTTTG CTATCAATGC CAGAACGCAG GTTGGTGCAG GGGAAGTCAT CACAGCTGAG
    TCTCCTGTCA GACTGGATGA AGTTTCTCCC ACGTCAGCTT ACACCAAGAA CCAAGTTGAC
    ATTGCTACAC AAGGCTGGTT TATTGGGCTC ATGTGTGCAG TTGCTCTACT GGTACTGATT
    TTGCTGATCG TTTGTTTTAT CAAAAGAAGC CGAGGAGGCA AATACCCAGT GCGAGAAAAG
    AAGGAGGTGG CGTTGGGACC AGATGATAAC AAAGAGGGGG ATGGCTCATT TGACTACAGT
    GATGAGGACA ACAAGCCCCT GCAGGGTAGT CAAACTTCGC TGGATGGAAC CATAAAGCAA
    CAAGAGAGTG ATGACAGCCT TGTTGATTAT GGAGAAGGAG GAGAGGGGCA GTTTAATGAA
    GATGGATCGT TTATAGGGCA GTATACAGTG AAAAAGGACA AAGATGAGAC TGAGGGAAAT
    GAAAGTTCAG AGGCCACATC TCCTGTAAAT GCTATCTATT CTCTGGCATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031752697.1
    CDS546..4076
    Translation

    Target ORF information:

    RefSeq Version XM_031896837.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neurofascin (nfasc), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896837.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGACAGATT TTCTAGAAAG GAGGTTAACG GTGATCCACT ACTACTGTCT CGGAAGCAAC 
    ATGCCATCAT GTTTAGTCCT TCTCCTGCTC TTTCTCCACC TAGTCTGCAC TATTAAAGTT
    CCACTGGACC CAAACATTCA GCGAGACCTA ATACAGCCCC CAACCATCAT CAAGCAGTCA
    GCAAAGAATT ATATTGTAGA TCCCCGTGAT AACATCCTAA TTGAGTGTGA AGCTCAGGGG
    AATCCTTACC CAATGTTTTC ATGGACAAAA AATGGGAAAT TTTACAATGT GGCAAAAGAC
    CCTCGAGTGT CATTCCGTCG ACGATCAGGC ACCCTTGAAA TAGAAATTCA TGGAGGAGGG
    CGGCCAGAGG ACTATGAGGG AGAATACCAA TGCTCTGCCC GCAACCAACA TGGAACAGCT
    TTATCAAATA AGATCTTACT TCAAGTCTCT CGATCCCCTT TGTGGCCCAA AGAAAACCTT
    GACCCGGTGG TGGTAAATGA AGGTGCCTTC TTGATCCTTC AGTGCAATCC CCCACATGGA
    CTTCCTCCTC CAGTCATATT TTGGATGAGC AGCTTGATGG AGCCTATTGT CCAGGACAAG
    AGGGTTTCTC AGGGACTGAA TGGGGACCTT TATTTCTCCA ACGTTCTGCG CCAGGATACT
    CAGACTGATT ATAGCTGCAA TGCACGCTCC CATTTCACAC ACACCATTCA ACAGAAGAAC
    CCATATACTC TAAAAGTGCT TACCACCAGA GGGGTACCTG AGAGAACCCC AACCTTCTTG
    CTTCCCACGG GGACTTCTAG CAGTAAACTG GTGTTACGCG ATGAGGACTT GCTACTGGAG
    TGTATTGCTT CTGGAGTACC AACGCCAGAC ATCATGTGGT ATAAGAAAGG AGGAGAGCTA
    CCCAGCAGGA AGCTGAAGTT TGAAAATTTT AACAAGACTC TACGCATAAT TGGAGTGTCT
    GAAGAGGACT CGGGGGAGTA TTTCTGCCAA GCTTCAAACA AAATGGGCAG CATTCGGCAC
    ACGATTTCTG TTCGGGTTAA GGCTGCTCCA TATTGGCTTG ATGAGCCCAA GAACTTGATT
    CTTGCACCAG GTGAGAACGG ACGTTTGGTA TGTCGTGCCA GTGGCAGCCC CAAACCTACA
    ATCCAATGGT TTATGAATGG AGATGCTACT GAAGTAGGTG CCCCAAATCC CAACTTCGAA
    ATCGCTGGTG ATACCATTAT TTTTAGAGAA GTACAGAGTG GCAACAGCGC TGTATACCAA
    TGCAATGCTT CTAATGAACA TGGTTATCTG TTAGCTAATG CCTTCGTCAG CATTCTGGAT
    GTTCCATCTC GGATGTTGGG CCCTCGTAAT CAGCTGATTC GAGTGATAGA ATACAACAGG
    ACCCAGCTAG ACTGCCCTTT CTTTGGCTCT CCTATACCTA CACTCCGCTG GTTTAAAAAT
    GGACAGGGTA GTAACCTCGA TGGTGGCAAT TACCGGGTGC ATGAAAATGG GACCCTTGAG
    ATTAATACTG TGAGGAAGGA GGACCAAGGC AAATACACAT GTGTTGCTAC CAATATTTTG
    GGCAATGCTG AAAATACTGT GCGGCTGGAG GTCAAAGAGC CAACTCGTAT TGTAAAGGGC
    CCTGAAGATC AGATTGCGAA GAGGGGCTCT TCTGTGCATC TGGACTGTCG AGTGCGCAGT
    GACTCCTCAC TCAGACTCAC TGTTACCTGG ATAAAAGATG ACACTCAGTT GTATATAAAC
    AGCAGAATGA AGAAGGATGA GGATGGACTT ACAATATTCG GTGTGGCAGA AAAGGATCAG
    GGAGCATATA CATGTGTTGC TAAAACTGAA TTAGACCAGG ACTCTTCTAA AGCTTATCTC
    ACGATACAGG ACAAACCAGA CCCCCCACAG GACCTGGAGC TAACTGAACC ATCAGTTAGG
    AGTGTTCGCC TCACCTGGGT TCCAGGGGAT GATAACAACA GTCCAATTAC AGGTTTCATT
    GTACAATTTG AGGAAGACAG GCATCAGCCC GGTACATGGC ACAACCTTGC CTCTGTCCCT
    GCTAATGTGA ACTCTGTCAT ACTGCAGCTT TCTCCTTATG TCAACTACCA GTTTCGTGTT
    CTTGCACTAA ATGAACTTGG GAGCAGTATG CCAAGCCCAT CTTCAGAACG TTTTCAGACA
    GCAGGAGCAC CTCCAGAAAC TAATCCAGGT GATGTGAAGG GAGCAGGGAC TAGCAAAAAC
    AATATGGAAA TCACATGGAC ACCACTGAAC TCCACTCAGG CGTATGGTCC AAACCTGCGC
    TACAACGTGA AGTGGCGCCA ACGTCACACA GGAGAACCTT GGAGCAATAT TACAGTGTGG
    GACAACAAGC ATGTGGTGTG GAATACACCA GTATACACCC AATATGAGCT AAGAGTTCAG
    GCGGAAAATG AATTTGGAAA GGCTCCAGAG CCAAGCACCA TGATTGGATA TTCTGGTGAA
    GACTATCCTA AGGCTGCACC CACTGACATT AAGCTTCGGG TCATGAACAG CACCGCCATC
    TCTCTTCAGT GGACACGTGT GGCACCTGCC TCTATCCAAG GAAGCCTACT GGAATACAGA
    GCTTATTTTT GGAGAGAAAG TAGTTTGCTG AAGGGTGTCT GGGTCTCACG AAAGAAGCAA
    CAACAGAGTT TTATGGGGGA CCGTGTGAGA GGCATCGTAT CTCAACTGAT TCCATACAGC
    ATCTACAAGC TGTACTTGGT GGCAGTGAAT GGCCGAGGGG ATGGCCCCAA AAGTGATGTT
    AAGGAATTCA CAACCCCAGA GGGAGTGCCG AGTGCTCCTA AATACTTTAG AGTATATGAG
    CCAAACCGGG AAATGATTGT TGTTGAATGG GGTCACCCAG AGCACCCGAA CGGAAACTTG
    ACTGCATACA ACCTCCGCAT CCAACCATTT AATGGCACCA GAACAGGGAA GGCTGTATTT
    GAAACTCTTT CACGTAATCA AACCAAATAT ACCCTGCAAA GAACAGATCC CGTGTCCCGG
    TATCGGTTTG CTATCAATGC CAGAACGCAG GTTGGTGCAG GGGAAGTCAT CACAGCTGAG
    TCTCCTGTCA GACTGGATGA AGTTTCTCCC ACGTCAGCTT ACACCAAGAA CCAAGTTGAC
    ATTGCTACAC AAGGCTGGTT TATTGGGCTC ATGTGTGCAG TTGCTCTACT GGTACTGATT
    TTGCTGATCG TTTGTTTTAT CAAAAGAAGC CGAGGAGGCA AATACCCAGT GCGAGAAAAG
    AAGGAGGTGG CGTTGGGACC AGATGATAAC AAAGAGGGGG ATGGCTCATT TGACTACAGT
    GATGAGGACA ACAAGCCCCT GCAGGGTAGT CAAACTTCGC TGGATGGAAC CATAAAGCAA
    CAAGAGAGTG ATGACAGCCT TGTTGATTAT GGAGAAGGAG GAGAGGGGCA GTTTAATGAA
    GATGGATCGT TTATAGGGCA GTATACAGTG AAAAAGGACA AAGATGAGAC TGAGGGAAAT
    GAAAGTTCAG AGGCCACATC TCCTGTAAAT GCTATCTATT CTCTGGCATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52229
    Clone ID Related Accession (Same CDS sequence) XM_031896838.1
    Accession Version XM_031896838.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3516bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neurofascin isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGACAGATT TTCTAGAAAG GAGGTTAACG GTGATCCACT ACTACTGTCT CGGAAGCAAC 
    ATGCCATCAT GTTTAGTCCT TCTCCTGCTC TTTCTCCACC TAGTCTGCAC TATTAAAGTT
    CCACTGGACC CAAACATTCA GCGAGACCTA ATACAGCCCC CAACCATCAT CAAGCAGTCA
    GCAAAGAATT ATATTGTAGA TCCCCGTGAT AACATCCTAA TTGAGTGTGA AGCTCAGGGG
    AATCCTTACC CAATGTTTTC ATGGACAAAA AATGGGAAAT TTTACAATGT GGCAAAAGAC
    CCTCGAGTGT CATTCCGTCG ACGATCAGGC ACCCTTGAAA TAGAAATTCA TGGAGGAGGG
    CGGCCAGAGG ACTATGAGGG AGAATACCAA TGCTCTGCCC GCAACCAACA TGGAACAGCT
    TTATCAAATA AGATCTTACT TCAAGTCTCT CGATCCCCTT TGTGGCCCAA AGAAAACCTT
    GACCCGGTGG TGGTAAATGA AGGTGCCTTC TTGATCCTTC AGTGCAATCC CCCACATGGA
    CTTCCTCCTC CAGTCATATT TTGGATGAGC AGCTTGATGG AGCCTATTGT CCAGGACAAG
    AGGGTTTCTC AGGGACTGAA TGGGGACCTT TATTTCTCCA ACGTTCTGCG CCAGGATACT
    CAGACTGATT ATAGCTGCAA TGCACGCTCC CATTTCACAC ACACCATTCA ACAGAAGAAC
    CCATATACTC TAAAAGTGCT TACCACCAGA GGGGTACCTG AGAGAACCCC AACCTTCTTG
    CTTCCCACGG GGACTTCTAG CAGTAAACTG GTGTTACGCG ATGAGGACTT GCTACTGGAG
    TGTATTGCTT CTGGAGTACC AACGCCAGAC ATCATGTGGT ATAAGAAAGG AGGAGAGCTA
    CCCAGCAGGA AGCTGAAGTT TGAAAATTTT AACAAGACTC TACGCATAAT TGGAGTGTCT
    GAAGAGGACT CGGGGGAGTA TTTCTGCCAA GCTTCAAACA AAATGGGCAG CATTCGGCAC
    ACGATTTCTG TTCGGGTTAA GGCTGCTCCA TATTGGCTTG ATGAGCCCAA GAACTTGATT
    CTTGCACCAG GTGAGAACGG ACGTTTGGTA TGTCGTGCCA GTGGCAGCCC CAAACCTACA
    ATCCAATGGT TTATGAATGG AGATGCTACT GAAGTAGGTG CCCCAAATCC CAACTTCGAA
    ATCGCTGGTG ATACCATTAT TTTTAGAGAA GTACAGAGTG GCAACAGCGC TGTATACCAA
    TGCAATGCTT CTAATGAACA TGGTTATCTG TTAGCTAATG CCTTCGTCAG CATTCTGGAT
    GTTCCATCTC GGATGTTGGG CCCTCGTAAT CAGCTGATTC GAGTGATAGA ATACAACAGG
    ACCCAGCTAG ACTGCCCTTT CTTTGGCTCT CCTATACCTA CACTCCGCTG GTTTAAAAAT
    GGACAGGGTA GTAACCTCGA TGGTGGCAAT TACCGGGTGC ATGAAAATGG GACCCTTGAG
    ATTAATACTG TGAGGAAGGA GGACCAAGGC AAATACACAT GTGTTGCTAC CAATATTTTG
    GGCAATGCTG AAAATACTGT GCGGCTGGAG GTCAAAGAGC CAACTCGTAT TGTAAAGGGC
    CCTGAAGATC AGATTGCGAA GAGGGGCTCT TCTGTGCATC TGGACTGTCG AGTGCGCAGT
    GACTCCTCAC TCAGACTCAC TGTTACCTGG ATAAAAGATG ACACTCAGTT GTATATAAAC
    AGCAGAATGA AGAAGGATGA GGATGGACTT ACAATATTCG GTGTGGCAGA AAAGGATCAG
    GGAGCATATA CATGTGTTGC TAAAACTGAA TTAGACCAGG ACTCTTCTAA AGCTTATCTC
    ACGATACAGG ACAAACCAGA CCCCCCACAG GACCTGGAGC TAACTGAACC ATCAGTTAGG
    AGTGTTCGCC TCACCTGGGT TCCAGGGGAT GATAACAACA GTCCAATTAC AGGTTTCATT
    GTACAATTTG AGGAAGACAG GCATCAGCCC GGTACATGGC ACAACCTTGC CTCTGTCCCT
    GCTAATGTGA ACTCTGTCAT ACTGCAGCTT TCTCCTTATG TCAACTACCA GTTTCGTGTT
    CTTGCACTAA ATGAACTTGG GAGCAGTATG CCAAGCCCAT CTTCAGAACG TTTTCAGACA
    GCAGGAGCAC CTCCAGAAAC TAATCCAGGT GATGTGAAGG GAGCAGGGAC TAGCAAAAAC
    AATATGGAAA TCACATGGAC ACCACTGAAC TCCACTCAGG CGTATGGTCC AAACCTGCGC
    TACAACGTGA AGTGGCGCCA ACGTCACACA GGAGAACCTT GGAGCAATAT TACAGTGTGG
    GACAACAAGC ATGTGGTGTG GAATACACCA GTATACACCC AATATGAGCT AAGAGTTCAG
    GCGGAAAATG AATTTGGAAA GGCTCCAGAG CCAAGCACCA TGATTGGATA TTCTGGTGAA
    GACTATCCTA AGGCTGCACC CACTGACATT AAGCTTCGGG TCATGAACAG CACCGCCATC
    TCTCTTCAGT GGACACGTGT GGCACCTGCC TCTATCCAAG GAAGCCTACT GGAATACAGA
    GCTTATTTTT GGAGAGAAAG TAGTTTGCTG AAGGGTGTCT GGGTCTCACG AAAGAAGCAA
    CAACAGAGTT TTATGGGGGA CCGTGTGAGA GGCATCGTAT CTCAACTGAT TCCATACAGC
    ATCTACAAGC TGTACTTGGT GGCAGTGAAT GGCCGAGGGG ATGGCCCCAA AAGTGATGTT
    AAGGAATTCA CAACCCCAGA GGGAGTGCCG AGTGCTCCTA AATACTTTAG AGTATATGAG
    CCAAACCGGG AAATGATTGT TGTTGAATGG GGTCACCCAG AGCACCCGAA CGGAAACTTG
    ACTGCATACA ACCTCCGCAT CCAACCATTT AATGGCACCA GAACAGGGAA GGCTGTATTT
    GAAACTCTTT CACGTAATCA AACCAAATAT ACCCTGCAAA GAACAGATCC CGTGTCCCGG
    TATCGGTTTG CTATCAATGC CAGAACGCAG GTTGGTGCAG GGGAAGTCAT CACAGCTGAG
    TCTCCTGTCA GACTGGATGA AGCTTACACC AAGAACCAAG TTGACATTGC TACACAAGGC
    TGGTTTATTG GGCTCATGTG TGCAGTTGCT CTACTGGTAC TGATTTTGCT GATCGTTTGT
    TTTATCAAAA GAAGCCGAGG AGGCAAATAC CCAGTGCGAG AAAAGAAGGA GGTGGCGTTG
    GGACCAGATG ATAACAAAGA GGGGGATGGC TCATTTGACT ACAGTGATGA GGACAACAAG
    CCCCTGCAGG GTAGTCAAAC TTCGCTGGAT GGAACCATAA AGCAACAAGA GAGTGATGAC
    AGCCTTGTTG ATTATGGAGA AGGAGGAGAG GGGCAGTTTA ATGAAGATGG ATCGTTTATA
    GGGCAGTATA CAGTGAAAAA GGACAAAGAT GAGACTGAGG GAAATGAAAG TTCAGAGGCC
    ACATCTCCTG TAAATGCTAT CTATTCTCTG GCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031752698.1
    CDS546..4061
    Translation

    Target ORF information:

    RefSeq Version XM_031896838.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neurofascin (nfasc), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896838.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGACAGATT TTCTAGAAAG GAGGTTAACG GTGATCCACT ACTACTGTCT CGGAAGCAAC 
    ATGCCATCAT GTTTAGTCCT TCTCCTGCTC TTTCTCCACC TAGTCTGCAC TATTAAAGTT
    CCACTGGACC CAAACATTCA GCGAGACCTA ATACAGCCCC CAACCATCAT CAAGCAGTCA
    GCAAAGAATT ATATTGTAGA TCCCCGTGAT AACATCCTAA TTGAGTGTGA AGCTCAGGGG
    AATCCTTACC CAATGTTTTC ATGGACAAAA AATGGGAAAT TTTACAATGT GGCAAAAGAC
    CCTCGAGTGT CATTCCGTCG ACGATCAGGC ACCCTTGAAA TAGAAATTCA TGGAGGAGGG
    CGGCCAGAGG ACTATGAGGG AGAATACCAA TGCTCTGCCC GCAACCAACA TGGAACAGCT
    TTATCAAATA AGATCTTACT TCAAGTCTCT CGATCCCCTT TGTGGCCCAA AGAAAACCTT
    GACCCGGTGG TGGTAAATGA AGGTGCCTTC TTGATCCTTC AGTGCAATCC CCCACATGGA
    CTTCCTCCTC CAGTCATATT TTGGATGAGC AGCTTGATGG AGCCTATTGT CCAGGACAAG
    AGGGTTTCTC AGGGACTGAA TGGGGACCTT TATTTCTCCA ACGTTCTGCG CCAGGATACT
    CAGACTGATT ATAGCTGCAA TGCACGCTCC CATTTCACAC ACACCATTCA ACAGAAGAAC
    CCATATACTC TAAAAGTGCT TACCACCAGA GGGGTACCTG AGAGAACCCC AACCTTCTTG
    CTTCCCACGG GGACTTCTAG CAGTAAACTG GTGTTACGCG ATGAGGACTT GCTACTGGAG
    TGTATTGCTT CTGGAGTACC AACGCCAGAC ATCATGTGGT ATAAGAAAGG AGGAGAGCTA
    CCCAGCAGGA AGCTGAAGTT TGAAAATTTT AACAAGACTC TACGCATAAT TGGAGTGTCT
    GAAGAGGACT CGGGGGAGTA TTTCTGCCAA GCTTCAAACA AAATGGGCAG CATTCGGCAC
    ACGATTTCTG TTCGGGTTAA GGCTGCTCCA TATTGGCTTG ATGAGCCCAA GAACTTGATT
    CTTGCACCAG GTGAGAACGG ACGTTTGGTA TGTCGTGCCA GTGGCAGCCC CAAACCTACA
    ATCCAATGGT TTATGAATGG AGATGCTACT GAAGTAGGTG CCCCAAATCC CAACTTCGAA
    ATCGCTGGTG ATACCATTAT TTTTAGAGAA GTACAGAGTG GCAACAGCGC TGTATACCAA
    TGCAATGCTT CTAATGAACA TGGTTATCTG TTAGCTAATG CCTTCGTCAG CATTCTGGAT
    GTTCCATCTC GGATGTTGGG CCCTCGTAAT CAGCTGATTC GAGTGATAGA ATACAACAGG
    ACCCAGCTAG ACTGCCCTTT CTTTGGCTCT CCTATACCTA CACTCCGCTG GTTTAAAAAT
    GGACAGGGTA GTAACCTCGA TGGTGGCAAT TACCGGGTGC ATGAAAATGG GACCCTTGAG
    ATTAATACTG TGAGGAAGGA GGACCAAGGC AAATACACAT GTGTTGCTAC CAATATTTTG
    GGCAATGCTG AAAATACTGT GCGGCTGGAG GTCAAAGAGC CAACTCGTAT TGTAAAGGGC
    CCTGAAGATC AGATTGCGAA GAGGGGCTCT TCTGTGCATC TGGACTGTCG AGTGCGCAGT
    GACTCCTCAC TCAGACTCAC TGTTACCTGG ATAAAAGATG ACACTCAGTT GTATATAAAC
    AGCAGAATGA AGAAGGATGA GGATGGACTT ACAATATTCG GTGTGGCAGA AAAGGATCAG
    GGAGCATATA CATGTGTTGC TAAAACTGAA TTAGACCAGG ACTCTTCTAA AGCTTATCTC
    ACGATACAGG ACAAACCAGA CCCCCCACAG GACCTGGAGC TAACTGAACC ATCAGTTAGG
    AGTGTTCGCC TCACCTGGGT TCCAGGGGAT GATAACAACA GTCCAATTAC AGGTTTCATT
    GTACAATTTG AGGAAGACAG GCATCAGCCC GGTACATGGC ACAACCTTGC CTCTGTCCCT
    GCTAATGTGA ACTCTGTCAT ACTGCAGCTT TCTCCTTATG TCAACTACCA GTTTCGTGTT
    CTTGCACTAA ATGAACTTGG GAGCAGTATG CCAAGCCCAT CTTCAGAACG TTTTCAGACA
    GCAGGAGCAC CTCCAGAAAC TAATCCAGGT GATGTGAAGG GAGCAGGGAC TAGCAAAAAC
    AATATGGAAA TCACATGGAC ACCACTGAAC TCCACTCAGG CGTATGGTCC AAACCTGCGC
    TACAACGTGA AGTGGCGCCA ACGTCACACA GGAGAACCTT GGAGCAATAT TACAGTGTGG
    GACAACAAGC ATGTGGTGTG GAATACACCA GTATACACCC AATATGAGCT AAGAGTTCAG
    GCGGAAAATG AATTTGGAAA GGCTCCAGAG CCAAGCACCA TGATTGGATA TTCTGGTGAA
    GACTATCCTA AGGCTGCACC CACTGACATT AAGCTTCGGG TCATGAACAG CACCGCCATC
    TCTCTTCAGT GGACACGTGT GGCACCTGCC TCTATCCAAG GAAGCCTACT GGAATACAGA
    GCTTATTTTT GGAGAGAAAG TAGTTTGCTG AAGGGTGTCT GGGTCTCACG AAAGAAGCAA
    CAACAGAGTT TTATGGGGGA CCGTGTGAGA GGCATCGTAT CTCAACTGAT TCCATACAGC
    ATCTACAAGC TGTACTTGGT GGCAGTGAAT GGCCGAGGGG ATGGCCCCAA AAGTGATGTT
    AAGGAATTCA CAACCCCAGA GGGAGTGCCG AGTGCTCCTA AATACTTTAG AGTATATGAG
    CCAAACCGGG AAATGATTGT TGTTGAATGG GGTCACCCAG AGCACCCGAA CGGAAACTTG
    ACTGCATACA ACCTCCGCAT CCAACCATTT AATGGCACCA GAACAGGGAA GGCTGTATTT
    GAAACTCTTT CACGTAATCA AACCAAATAT ACCCTGCAAA GAACAGATCC CGTGTCCCGG
    TATCGGTTTG CTATCAATGC CAGAACGCAG GTTGGTGCAG GGGAAGTCAT CACAGCTGAG
    TCTCCTGTCA GACTGGATGA AGCTTACACC AAGAACCAAG TTGACATTGC TACACAAGGC
    TGGTTTATTG GGCTCATGTG TGCAGTTGCT CTACTGGTAC TGATTTTGCT GATCGTTTGT
    TTTATCAAAA GAAGCCGAGG AGGCAAATAC CCAGTGCGAG AAAAGAAGGA GGTGGCGTTG
    GGACCAGATG ATAACAAAGA GGGGGATGGC TCATTTGACT ACAGTGATGA GGACAACAAG
    CCCCTGCAGG GTAGTCAAAC TTCGCTGGAT GGAACCATAA AGCAACAAGA GAGTGATGAC
    AGCCTTGTTG ATTATGGAGA AGGAGGAGAG GGGCAGTTTA ATGAAGATGG ATCGTTTATA
    GGGCAGTATA CAGTGAAAAA GGACAAAGAT GAGACTGAGG GAAATGAAAG TTCAGAGGCC
    ACATCTCCTG TAAATGCTAT CTATTCTCTG GCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52230
    Clone ID Related Accession (Same CDS sequence) XM_031896842.1
    Accession Version XM_031896842.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3195bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neurofascin isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGACAGATT TTCTAGAAAG GAGGTTAACG GTGATCCACT ACTACTGTCT CGGAAGCAAC 
    ATGCCATCAT GTTTAGTCCT TCTCCTGCTC TTTCTCCACC TAGTCTGCAC TATTAAAGTT
    CCACTGGACC CAAACATTCA GCGAGACCTA ATACAGCCCC CAACCATCAT CAAGCAGTCA
    GCAAAGAATT ATATTGTAGA TCCCCGTGAT AACATCCTAA TTGAGTGTGA AGCTCAGGGG
    AATCCTTACC CAATGTTTTC ATGGACAAAA AATGGGAAAT TTTACAATGT GGCAAAAGAC
    CCTCGAGTGT CATTCCGTCG ACGATCAGGC ACCCTTGAAA TAGAAATTCA TGGAGGAGGG
    CGGCCAGAGG ACTATGAGGG AGAATACCAA TGCTCTGCCC GCAACCAACA TGGAACAGCT
    TTATCAAATA AGATCTTACT TCAAGTCTCT CGATCCCCTT TGTGGCCCAA AGAAAACCTT
    GACCCGGTGG TGGTAAATGA AGGTGCCTTC TTGATCCTTC AGTGCAATCC CCCACATGGA
    CTTCCTCCTC CAGTCATATT TTGGATGAGC AGCTTGATGG AGCCTATTGT CCAGGACAAG
    AGGGTTTCTC AGGGACTGAA TGGGGACCTT TATTTCTCCA ACGTTCTGCG CCAGGATACT
    CAGACTGATT ATAGCTGCAA TGCACGCTCC CATTTCACAC ACACCATTCA ACAGAAGAAC
    CCATATACTC TAAAAGTGCT TACCACCAGA GGGGTACCTG AGAGAACCCC AACCTTCTTG
    CTTCCCACGG GGACTTCTAG CAGTAAACTG GTGTTACGCG ATGAGGACTT GCTACTGGAG
    TGTATTGCTT CTGGAGTACC AACGCCAGAC ATCATGTGGT ATAAGAAAGG AGGAGAGCTA
    CCCAGCAGGA AGCTGAAGTT TGAAAATTTT AACAAGACTC TACGCATAAT TGGAGTGTCT
    GAAGAGGACT CGGGGGAGTA TTTCTGCCAA GCTTCAAACA AAATGGGCAG CATTCGGCAC
    ACGATTTCTG TTCGGGTTAA GGCTGCTCCA TATTGGCTTG ATGAGCCCAA GAACTTGATT
    CTTGCACCAG GTGAGAACGG ACGTTTGGTA TGTCGTGCCA GTGGCAGCCC CAAACCTACA
    ATCCAATGGT TTATGAATGG AGATGCTACT GAAGTAGGTG CCCCAAATCC CAACTTCGAA
    ATCGCTGGTG ATACCATTAT TTTTAGAGAA GTACAGAGTG GCAACAGCGC TGTATACCAA
    TGCAATGCTT CTAATGAACA TGGTTATCTG TTAGCTAATG CCTTCGTCAG CATTCTGGAT
    GTTCCATCTC GGATGTTGGG CCCTCGTAAT CAGCTGATTC GAGTGATAGA ATACAACAGG
    ACCCAGCTAG ACTGCCCTTT CTTTGGCTCT CCTATACCTA CACTCCGCTG GTTTAAAAAT
    GGACAGGGTA GTAACCTCGA TGGTGGCAAT TACCGGGTGC ATGAAAATGG GACCCTTGAG
    ATTAATACTG TGAGGAAGGA GGACCAAGGC AAATACACAT GTGTTGCTAC CAATATTTTG
    GGCAATGCTG AAAATACTGT GCGGCTGGAG GTCAAAGAGC CAACTCGTAT TGTAAAGGGC
    CCTGAAGATC AGATTGCGAA GAGGGGCTCT TCTGTGCATC TGGACTGTCG AGTGCGCAGT
    GACTCCTCAC TCAGACTCAC TGTTACCTGG ATAAAAGATG ACACTCAGTT GTATATAAAC
    AGCAGAATGA AGAAGGATGA GGATGGACTT ACAATATTCG GTGTGGCAGA AAAGGATCAG
    GGAGCATATA CATGTGTTGC TAAAACTGAA TTAGACCAGG ACTCTTCTAA AGCTTATCTC
    ACGATACAGG ACAAACCAGA CCCCCCACAG GACCTGGAGC TAACTGAACC ATCAGTTAGG
    AGTGTTCGCC TCACCTGGGT TCCAGGGGAT GATAACAACA GTCCAATTAC AGGTTTCATT
    GTACAATTTG AGGAAGACAG GCATCAGCCC GGTACATGGC ACAACCTTGC CTCTGTCCCT
    GCTAATGTGA ACTCTGTCAT ACTGCAGCTT TCTCCTTATG TCAACTACCA GTTTCGTGTT
    CTTGCACTAA ATGAACTTGG GAGCAGTATG CCAAGCCCAT CTTCAGAACG TTTTCAGACA
    GCAGGAGCAC CTCCAGAAAC TAATCCAGGT GATGTGAAGG GAGCAGGGAC TAGCAAAAAC
    AATATGGAAA TCACATGGAC ACCACTGAAC TCCACTCAGG CGTATGGTCC AAACCTGCGC
    TACAACGTGA AGTGGCGCCA ACGTCACACA GGAGAACCTT GGAGCAATAT TACAGTGTGG
    GACAACAAGC ATGTGGTGTG GAATACACCA GTATACACCC AATATGAGCT AAGAGTTCAG
    GCGGAAAATG AATTTGGAAA GGCTCCAGAG CCAAGCACCA TGATTGGATA TTCTGGTGAA
    GACTTGCCGA GTGCTCCTAA ATACTTTAGA GTATATGAGC CAAACCGGGA AATGATTGTT
    GTTGAATGGG GTCACCCAGA GCACCCGAAC GGAAACTTGA CTGCATACAA CCTCCGCATC
    CAACCATTTA ATGGCACCAG AACAGGGAAG GCTGTATTTG AAACTCTTTC ACGTAATCAA
    ACCAAATATA CCCTGCAAAG AACAGATCCC GTGTCCCGGT ATCGGTTTGC TATCAATGCC
    AGAACGCAGG TTGGTGCAGG GGAAGTCATC ACAGCTGAGT CTCCTGTCAG ACTGGATGAA
    GCTTACACCA AGAACCAAGT TGACATTGCT ACACAAGGCT GGTTTATTGG GCTCATGTGT
    GCAGTTGCTC TACTGGTACT GATTTTGCTG ATCGTTTGTT TTATCAAAAG AAGCCGAGGA
    GGCAAATACC CAGTGCGAGA AAAGAAGGAG GTGGCGTTGG GACCAGATGA TAACAAAGAG
    GGGGATGGCT CATTTGACTA CAGTGATGAG GACAACAAGC CCCTGCAGGG TAGTCAAACT
    TCGCTGGATG GAACCATAAA GCAACAAGAG AGTGATGACA GCCTTGTTGA TTATGGAGAA
    GGAGGAGAGG GGCAGTTTAA TGAAGATGGA TCGTTTATAG GGCAGTATAC AGTGAAAAAG
    GACAAAGATG AGACTGAGGG AAATGAAAGT TCAGAGGCCA CATCTCCTGT AAATGCTATC
    TATTCTCTGG CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031752702.1
    CDS543..3737
    Translation

    Target ORF information:

    RefSeq Version XM_031896842.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neurofascin (nfasc), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896842.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGACAGATT TTCTAGAAAG GAGGTTAACG GTGATCCACT ACTACTGTCT CGGAAGCAAC 
    ATGCCATCAT GTTTAGTCCT TCTCCTGCTC TTTCTCCACC TAGTCTGCAC TATTAAAGTT
    CCACTGGACC CAAACATTCA GCGAGACCTA ATACAGCCCC CAACCATCAT CAAGCAGTCA
    GCAAAGAATT ATATTGTAGA TCCCCGTGAT AACATCCTAA TTGAGTGTGA AGCTCAGGGG
    AATCCTTACC CAATGTTTTC ATGGACAAAA AATGGGAAAT TTTACAATGT GGCAAAAGAC
    CCTCGAGTGT CATTCCGTCG ACGATCAGGC ACCCTTGAAA TAGAAATTCA TGGAGGAGGG
    CGGCCAGAGG ACTATGAGGG AGAATACCAA TGCTCTGCCC GCAACCAACA TGGAACAGCT
    TTATCAAATA AGATCTTACT TCAAGTCTCT CGATCCCCTT TGTGGCCCAA AGAAAACCTT
    GACCCGGTGG TGGTAAATGA AGGTGCCTTC TTGATCCTTC AGTGCAATCC CCCACATGGA
    CTTCCTCCTC CAGTCATATT TTGGATGAGC AGCTTGATGG AGCCTATTGT CCAGGACAAG
    AGGGTTTCTC AGGGACTGAA TGGGGACCTT TATTTCTCCA ACGTTCTGCG CCAGGATACT
    CAGACTGATT ATAGCTGCAA TGCACGCTCC CATTTCACAC ACACCATTCA ACAGAAGAAC
    CCATATACTC TAAAAGTGCT TACCACCAGA GGGGTACCTG AGAGAACCCC AACCTTCTTG
    CTTCCCACGG GGACTTCTAG CAGTAAACTG GTGTTACGCG ATGAGGACTT GCTACTGGAG
    TGTATTGCTT CTGGAGTACC AACGCCAGAC ATCATGTGGT ATAAGAAAGG AGGAGAGCTA
    CCCAGCAGGA AGCTGAAGTT TGAAAATTTT AACAAGACTC TACGCATAAT TGGAGTGTCT
    GAAGAGGACT CGGGGGAGTA TTTCTGCCAA GCTTCAAACA AAATGGGCAG CATTCGGCAC
    ACGATTTCTG TTCGGGTTAA GGCTGCTCCA TATTGGCTTG ATGAGCCCAA GAACTTGATT
    CTTGCACCAG GTGAGAACGG ACGTTTGGTA TGTCGTGCCA GTGGCAGCCC CAAACCTACA
    ATCCAATGGT TTATGAATGG AGATGCTACT GAAGTAGGTG CCCCAAATCC CAACTTCGAA
    ATCGCTGGTG ATACCATTAT TTTTAGAGAA GTACAGAGTG GCAACAGCGC TGTATACCAA
    TGCAATGCTT CTAATGAACA TGGTTATCTG TTAGCTAATG CCTTCGTCAG CATTCTGGAT
    GTTCCATCTC GGATGTTGGG CCCTCGTAAT CAGCTGATTC GAGTGATAGA ATACAACAGG
    ACCCAGCTAG ACTGCCCTTT CTTTGGCTCT CCTATACCTA CACTCCGCTG GTTTAAAAAT
    GGACAGGGTA GTAACCTCGA TGGTGGCAAT TACCGGGTGC ATGAAAATGG GACCCTTGAG
    ATTAATACTG TGAGGAAGGA GGACCAAGGC AAATACACAT GTGTTGCTAC CAATATTTTG
    GGCAATGCTG AAAATACTGT GCGGCTGGAG GTCAAAGAGC CAACTCGTAT TGTAAAGGGC
    CCTGAAGATC AGATTGCGAA GAGGGGCTCT TCTGTGCATC TGGACTGTCG AGTGCGCAGT
    GACTCCTCAC TCAGACTCAC TGTTACCTGG ATAAAAGATG ACACTCAGTT GTATATAAAC
    AGCAGAATGA AGAAGGATGA GGATGGACTT ACAATATTCG GTGTGGCAGA AAAGGATCAG
    GGAGCATATA CATGTGTTGC TAAAACTGAA TTAGACCAGG ACTCTTCTAA AGCTTATCTC
    ACGATACAGG ACAAACCAGA CCCCCCACAG GACCTGGAGC TAACTGAACC ATCAGTTAGG
    AGTGTTCGCC TCACCTGGGT TCCAGGGGAT GATAACAACA GTCCAATTAC AGGTTTCATT
    GTACAATTTG AGGAAGACAG GCATCAGCCC GGTACATGGC ACAACCTTGC CTCTGTCCCT
    GCTAATGTGA ACTCTGTCAT ACTGCAGCTT TCTCCTTATG TCAACTACCA GTTTCGTGTT
    CTTGCACTAA ATGAACTTGG GAGCAGTATG CCAAGCCCAT CTTCAGAACG TTTTCAGACA
    GCAGGAGCAC CTCCAGAAAC TAATCCAGGT GATGTGAAGG GAGCAGGGAC TAGCAAAAAC
    AATATGGAAA TCACATGGAC ACCACTGAAC TCCACTCAGG CGTATGGTCC AAACCTGCGC
    TACAACGTGA AGTGGCGCCA ACGTCACACA GGAGAACCTT GGAGCAATAT TACAGTGTGG
    GACAACAAGC ATGTGGTGTG GAATACACCA GTATACACCC AATATGAGCT AAGAGTTCAG
    GCGGAAAATG AATTTGGAAA GGCTCCAGAG CCAAGCACCA TGATTGGATA TTCTGGTGAA
    GACTTGCCGA GTGCTCCTAA ATACTTTAGA GTATATGAGC CAAACCGGGA AATGATTGTT
    GTTGAATGGG GTCACCCAGA GCACCCGAAC GGAAACTTGA CTGCATACAA CCTCCGCATC
    CAACCATTTA ATGGCACCAG AACAGGGAAG GCTGTATTTG AAACTCTTTC ACGTAATCAA
    ACCAAATATA CCCTGCAAAG AACAGATCCC GTGTCCCGGT ATCGGTTTGC TATCAATGCC
    AGAACGCAGG TTGGTGCAGG GGAAGTCATC ACAGCTGAGT CTCCTGTCAG ACTGGATGAA
    GCTTACACCA AGAACCAAGT TGACATTGCT ACACAAGGCT GGTTTATTGG GCTCATGTGT
    GCAGTTGCTC TACTGGTACT GATTTTGCTG ATCGTTTGTT TTATCAAAAG AAGCCGAGGA
    GGCAAATACC CAGTGCGAGA AAAGAAGGAG GTGGCGTTGG GACCAGATGA TAACAAAGAG
    GGGGATGGCT CATTTGACTA CAGTGATGAG GACAACAAGC CCCTGCAGGG TAGTCAAACT
    TCGCTGGATG GAACCATAAA GCAACAAGAG AGTGATGACA GCCTTGTTGA TTATGGAGAA
    GGAGGAGAGG GGCAGTTTAA TGAAGATGGA TCGTTTATAG GGCAGTATAC AGTGAAAAAG
    GACAAAGATG AGACTGAGGG AAATGAAAGT TCAGAGGCCA CATCTCCTGT AAATGCTATC
    TATTCTCTGG CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa03810
    Clone ID Related Accession (Same CDS sequence) XM_004910697.4 , XM_004910697.3
    Accession Version XM_004910697.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3999bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neurofascin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004910697.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGACAGATT TTCTAGAAAG GAGGTTAACG GTGATCCACT ACTACTGTCT CGGAAGCAAC 
    ATGCCATCAT GTTTAGTCCT TCTCCTGCTC TTTCTCCACC TAGTCTGCAC TATTAAAGTT
    CCACTGGACC TAATACAGCC CCCAACCATC ATCAAGCAGT CAGCAAAGAA TTATATTGTA
    GATCCCCGTG ATAACATCCT AATTGAGTGT GAAGCTCAGG GGAATCCTTA CCCAATGTTT
    TCATGGACAA AAAATGGGAA ATTTTACAAT GTGGCAAAAG ACCCTCGAGT GTCATTCCGT
    CGACGATCAG GCACCCTTGA AATAGAAATT CATGGAGGAG GGCGGCCAGA GGACTATGAG
    GGAGAATACC AATGCTCTGC CCGCAACCAA CATGGAACAG CTTTATCAAA TAAGATCTTA
    CTTCAAGTCT CTCGATCCCC TTTGTGGCCC AAAGAAAACC TTGACCCGGT GGTGGTAAAT
    GAAGGTGCCT TCTTGATCCT TCAGTGCAAT CCCCCACATG GACTTCCTCC TCCAGTCATA
    TTTTGGATGA GCAGCTTGAT GGAGCCTATT GTCCAGGACA AGAGGGTTTC TCAGGGACTG
    AATGGGGACC TTTATTTCTC CAACGTTCTG CGCCAGGATA CTCAGACTGA TTATAGCTGC
    AATGCACGCT CCCATTTCAC ACACACCATT CAACAGAAGA ACCCATATAC TCTAAAAGTG
    CTTACCACCA GAGGGGTACC TGAGAGAACC CCAACCTTCT TGCTTCCCAC GGGGACTTCT
    AGCAGTAAAC TGGTGTTACG CGATGAGGAC TTGCTACTGG AGTGTATTGC TTCTGGAGTA
    CCAACGCCAG ACATCATGTG GTATAAGAAA GGAGGAGAGC TACCCAGCAG GAAGCTGAAG
    TTTGAAAATT TTAACAAGAC TCTACGCATA ATTGGAGTGT CTGAAGAGGA CTCGGGGGAG
    TATTTCTGCC AAGCTTCAAA CAAAATGGGC AGCATTCGGC ACACGATTTC TGTTCGGGTT
    AAGGCTGCTC CATATTGGCT TGATGAGCCC AAGAACTTGA TTCTTGCACC AGGTGAGAAC
    GGACGTTTGG TATGTCGTGC CAGTGGCAGC CCCAAACCTA CAATCCAATG GTTTATGAAT
    GGAGATGCTA CTGAAGTAGG TGCCCCAAAT CCCAACTTCG AAATCGCTGG TGATACCATT
    ATTTTTAGAG AAGTACAGAG TGGCAACAGC GCTGTATACC AATGCAATGC TTCTAATGAA
    CATGGTTATC TGTTAGCTAA TGCCTTCGTC AGCATTCTGG ATGTTCCATC TCGGATGTTG
    GGCCCTCGTA ATCAGCTGAT TCGAGTGATA GAATACAACA GGACCCAGCT AGACTGCCCT
    TTCTTTGGCT CTCCTATACC TACACTCCGC TGGTTTAAAA ATGGACAGGG TAGTAACCTC
    GATGGTGGCA ATTACCGGGT GCATGAAAAT GGGACCCTTG AGATTAATAC TGTGAGGAAG
    GAGGACCAAG GCAAATACAC ATGTGTTGCT ACCAATATTT TGGGCAATGC TGAAAATACT
    GTGCGGCTGG AGGTCAAAGA GCCAACTCGT ATTGTAAAGG GCCCTGAAGA TCAGATTGCG
    AAGAGGGGCT CTTCTGTGCA TCTGGACTGT CGAGTGCGCA GTGACTCCTC ACTCAGACTC
    ACTGTTACCT GGATAAAAGA TGACACTCAG TTGTATATAA ACAGCAGAAT GAAGAAGGAT
    GAGGATGGAC TTACAATATT CGGTGTGGCA GAAAAGGATC AGGGAGCATA TACATGTGTT
    GCTAAAACTG AATTAGACCA GGACTCTTCT AAAGCTTATC TCACGATACA GGAAACTTCC
    TCACCTACTA GCCGACTGAC CTCCCAGCTT ATAGACAAAC CAGACCCCCC ACAGGACCTG
    GAGCTAACTG AACCATCAGT TAGGAGTGTT CGCCTCACCT GGGTTCCAGG GGATGATAAC
    AACAGTCCAA TTACAGGTTT CATTGTACAA TTTGAGGAAG ACAGGCATCA GCCCGGTACA
    TGGCACAACC TTGCCTCTGT CCCTGCTAAT GTGAACTCTG TCATACTGCA GCTTTCTCCT
    TATGTCAACT ACCAGTTTCG TGTTCTTGCA CTAAATGAAC TTGGGAGCAG TATGCCAAGC
    CCATCTTCAG AACGTTTTCA GACAGCAGGA GCACCTCCAG AAACTAATCC AGGTGATGTG
    AAGGGAGCAG GGACTAGCAA AAACAATATG GAAATCACAT GGACACCACT GAACTCCACT
    CAGGCGTATG GTCCAAACCT GCGCTACAAC GTGAAGTGGC GCCAACGTCA CACAGGAGAA
    CCTTGGAGCA ATATTACAGT GTGGGACAAC AAGCATGTGG TGTGGAATAC ACCAGTATAC
    ACCCAATATG AGCTAAGAGT TCAGGCGGAA AATGAATTTG GAAAGGCTCC AGAGCCAAGC
    ACCATGATTG GATATTCTGG TGAAGACTAT CCTAAGGCTG CACCCACTGA CATTAAGCTT
    CGGGTCATGA ACAGCACCGC CATCTCTCTT CAGTGGACAC GTGTGGCACC TGCCTCTATC
    CAAGGAAGCC TACTGGAATA CAGAGCTTAT TTTTGGAGAG AAAGTAGTTT GCTGAAGGGT
    GTCTGGGTCT CACGAAAGAA GCAACAACAG AGTTTTATGG GGGACCGTGT GAGAGGCATC
    GTATCTCAAC TGATTCCATA CAGCATCTAC AAGCTGTACT TGGTGGCAGT GAATGGCCGA
    GGGGATGGCC CCAAAAGTGA TGTTAAGGAA TTCACAACCC CAGAGGGAGT GCCGAGTGCT
    CCTAAATACT TTAGAGTATA TGAGCCAAAC CGGGAAATGA TTGTTGTTGA ATGGGGTCAC
    CCAGAGCACC CGAACGGAAA CTTGACTGCA TACAACCTCC GCATCCAACC ATTTAATGGC
    ACCAGAACAG GGAAGGCTGT ATTTGAAACT CTTTCACGTA ATCAAACCAA ATATACCCTG
    CAAAGAACAG ATCCCGTGTC CCGGTATCGG TTTGCTATCA ATGCCAGAAC GCAGGTTGGT
    GCAGGGGAAG TCATCACAGC TGAGTCTCCT GTCAGACTGG ATGAAGTTTC TCCCACGTCA
    GTGTCTTCTA AGTCTCCTGC ACCTGTCACA GATGCTCCCA CAGAGGTCCC AACTATTCCC
    CCAACCTCAT CTGGTGCCAC CACTCTTCTA GCTACAACCA GTACAACCAG TGTCACAACG
    ACTACTGCTG GTACAGAAAG TTCGCTGGTC GCCATTACTA GAATTCCTAA TATAGCCACC
    ACTGGACCAT CTCTATGGAA CCTAACAGCA TTACCCAACA CTTACTGGGC AAACATCACC
    TGGATGCATA ACTTAGAGCC AGGCTCTGTG TTTATGGTTG AGTATACTGA CAGTAATAAT
    ACAGTGAAAA CTGTTCCTGT TAAGGCTCAT GTCCCTTCTA TCCAACTGTT TGATCTGGTG
    CCTGGGATGA CCTACAGGGT GCGGGTTTAC TCCCAGGAGC ACCCGTCAGT CACAAGCCTA
    TATGTGACAT TTGATACCAG CTCAGCTTAC ACCAAGAACC AAGTTGACAT TGCTACACAA
    GGCTGGTTTA TTGGGCTCAT GTGTGCAGTT GCTCTACTGG TACTGATTTT GCTGATCGTT
    TGTTTTATCA AAAGAAGCCG AGGAGGCAAA TACCCAGTGC GAGAAAAGAA GGAGGTGGCG
    TTGGGACCAG ATGATAACAA AGAGGGGGAT GGCTCATTTG ACTACAGTGA TGAGGACAAC
    AAGCCCCTGC AGGGTAGTCA AACTTCGCTG GATGGAACCA TAAAGCAACA AGAGAGTGAT
    GACAGCCTTG TTGATTATGG AGAAGGAGGA GAGGGGCAGT TTAATGAAGA TGGATCGTTT
    ATAGGGCAGT ATACAGTGAA AAAGGACAAA GATGAGACTG AGGGAAATGA AAGTTCAGAG
    GCCACATCTC CTGTAAATGC TATCTATTCT CTGGCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004910754.1
    CDS554..4552
    Translation

    Target ORF information:

    RefSeq Version XM_004910697.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neurofascin (nfasc), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004910697.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGACAGATT TTCTAGAAAG GAGGTTAACG GTGATCCACT ACTACTGTCT CGGAAGCAAC 
    ATGCCATCAT GTTTAGTCCT TCTCCTGCTC TTTCTCCACC TAGTCTGCAC TATTAAAGTT
    CCACTGGACC TAATACAGCC CCCAACCATC ATCAAGCAGT CAGCAAAGAA TTATATTGTA
    GATCCCCGTG ATAACATCCT AATTGAGTGT GAAGCTCAGG GGAATCCTTA CCCAATGTTT
    TCATGGACAA AAAATGGGAA ATTTTACAAT GTGGCAAAAG ACCCTCGAGT GTCATTCCGT
    CGACGATCAG GCACCCTTGA AATAGAAATT CATGGAGGAG GGCGGCCAGA GGACTATGAG
    GGAGAATACC AATGCTCTGC CCGCAACCAA CATGGAACAG CTTTATCAAA TAAGATCTTA
    CTTCAAGTCT CTCGATCCCC TTTGTGGCCC AAAGAAAACC TTGACCCGGT GGTGGTAAAT
    GAAGGTGCCT TCTTGATCCT TCAGTGCAAT CCCCCACATG GACTTCCTCC TCCAGTCATA
    TTTTGGATGA GCAGCTTGAT GGAGCCTATT GTCCAGGACA AGAGGGTTTC TCAGGGACTG
    AATGGGGACC TTTATTTCTC CAACGTTCTG CGCCAGGATA CTCAGACTGA TTATAGCTGC
    AATGCACGCT CCCATTTCAC ACACACCATT CAACAGAAGA ACCCATATAC TCTAAAAGTG
    CTTACCACCA GAGGGGTACC TGAGAGAACC CCAACCTTCT TGCTTCCCAC GGGGACTTCT
    AGCAGTAAAC TGGTGTTACG CGATGAGGAC TTGCTACTGG AGTGTATTGC TTCTGGAGTA
    CCAACGCCAG ACATCATGTG GTATAAGAAA GGAGGAGAGC TACCCAGCAG GAAGCTGAAG
    TTTGAAAATT TTAACAAGAC TCTACGCATA ATTGGAGTGT CTGAAGAGGA CTCGGGGGAG
    TATTTCTGCC AAGCTTCAAA CAAAATGGGC AGCATTCGGC ACACGATTTC TGTTCGGGTT
    AAGGCTGCTC CATATTGGCT TGATGAGCCC AAGAACTTGA TTCTTGCACC AGGTGAGAAC
    GGACGTTTGG TATGTCGTGC CAGTGGCAGC CCCAAACCTA CAATCCAATG GTTTATGAAT
    GGAGATGCTA CTGAAGTAGG TGCCCCAAAT CCCAACTTCG AAATCGCTGG TGATACCATT
    ATTTTTAGAG AAGTACAGAG TGGCAACAGC GCTGTATACC AATGCAATGC TTCTAATGAA
    CATGGTTATC TGTTAGCTAA TGCCTTCGTC AGCATTCTGG ATGTTCCATC TCGGATGTTG
    GGCCCTCGTA ATCAGCTGAT TCGAGTGATA GAATACAACA GGACCCAGCT AGACTGCCCT
    TTCTTTGGCT CTCCTATACC TACACTCCGC TGGTTTAAAA ATGGACAGGG TAGTAACCTC
    GATGGTGGCA ATTACCGGGT GCATGAAAAT GGGACCCTTG AGATTAATAC TGTGAGGAAG
    GAGGACCAAG GCAAATACAC ATGTGTTGCT ACCAATATTT TGGGCAATGC TGAAAATACT
    GTGCGGCTGG AGGTCAAAGA GCCAACTCGT ATTGTAAAGG GCCCTGAAGA TCAGATTGCG
    AAGAGGGGCT CTTCTGTGCA TCTGGACTGT CGAGTGCGCA GTGACTCCTC ACTCAGACTC
    ACTGTTACCT GGATAAAAGA TGACACTCAG TTGTATATAA ACAGCAGAAT GAAGAAGGAT
    GAGGATGGAC TTACAATATT CGGTGTGGCA GAAAAGGATC AGGGAGCATA TACATGTGTT
    GCTAAAACTG AATTAGACCA GGACTCTTCT AAAGCTTATC TCACGATACA GGAAACTTCC
    TCACCTACTA GCCGACTGAC CTCCCAGCTT ATAGACAAAC CAGACCCCCC ACAGGACCTG
    GAGCTAACTG AACCATCAGT TAGGAGTGTT CGCCTCACCT GGGTTCCAGG GGATGATAAC
    AACAGTCCAA TTACAGGTTT CATTGTACAA TTTGAGGAAG ACAGGCATCA GCCCGGTACA
    TGGCACAACC TTGCCTCTGT CCCTGCTAAT GTGAACTCTG TCATACTGCA GCTTTCTCCT
    TATGTCAACT ACCAGTTTCG TGTTCTTGCA CTAAATGAAC TTGGGAGCAG TATGCCAAGC
    CCATCTTCAG AACGTTTTCA GACAGCAGGA GCACCTCCAG AAACTAATCC AGGTGATGTG
    AAGGGAGCAG GGACTAGCAA AAACAATATG GAAATCACAT GGACACCACT GAACTCCACT
    CAGGCGTATG GTCCAAACCT GCGCTACAAC GTGAAGTGGC GCCAACGTCA CACAGGAGAA
    CCTTGGAGCA ATATTACAGT GTGGGACAAC AAGCATGTGG TGTGGAATAC ACCAGTATAC
    ACCCAATATG AGCTAAGAGT TCAGGCGGAA AATGAATTTG GAAAGGCTCC AGAGCCAAGC
    ACCATGATTG GATATTCTGG TGAAGACTAT CCTAAGGCTG CACCCACTGA CATTAAGCTT
    CGGGTCATGA ACAGCACCGC CATCTCTCTT CAGTGGACAC GTGTGGCACC TGCCTCTATC
    CAAGGAAGCC TACTGGAATA CAGAGCTTAT TTTTGGAGAG AAAGTAGTTT GCTGAAGGGT
    GTCTGGGTCT CACGAAAGAA GCAACAACAG AGTTTTATGG GGGACCGTGT GAGAGGCATC
    GTATCTCAAC TGATTCCATA CAGCATCTAC AAGCTGTACT TGGTGGCAGT GAATGGCCGA
    GGGGATGGCC CCAAAAGTGA TGTTAAGGAA TTCACAACCC CAGAGGGAGT GCCGAGTGCT
    CCTAAATACT TTAGAGTATA TGAGCCAAAC CGGGAAATGA TTGTTGTTGA ATGGGGTCAC
    CCAGAGCACC CGAACGGAAA CTTGACTGCA TACAACCTCC GCATCCAACC ATTTAATGGC
    ACCAGAACAG GGAAGGCTGT ATTTGAAACT CTTTCACGTA ATCAAACCAA ATATACCCTG
    CAAAGAACAG ATCCCGTGTC CCGGTATCGG TTTGCTATCA ATGCCAGAAC GCAGGTTGGT
    GCAGGGGAAG TCATCACAGC TGAGTCTCCT GTCAGACTGG ATGAAGTTTC TCCCACGTCA
    GTGTCTTCTA AGTCTCCTGC ACCTGTCACA GATGCTCCCA CAGAGGTCCC AACTATTCCC
    CCAACCTCAT CTGGTGCCAC CACTCTTCTA GCTACAACCA GTACAACCAG TGTCACAACG
    ACTACTGCTG GTACAGAAAG TTCGCTGGTC GCCATTACTA GAATTCCTAA TATAGCCACC
    ACTGGACCAT CTCTATGGAA CCTAACAGCA TTACCCAACA CTTACTGGGC AAACATCACC
    TGGATGCATA ACTTAGAGCC AGGCTCTGTG TTTATGGTTG AGTATACTGA CAGTAATAAT
    ACAGTGAAAA CTGTTCCTGT TAAGGCTCAT GTCCCTTCTA TCCAACTGTT TGATCTGGTG
    CCTGGGATGA CCTACAGGGT GCGGGTTTAC TCCCAGGAGC ACCCGTCAGT CACAAGCCTA
    TATGTGACAT TTGATACCAG CTCAGCTTAC ACCAAGAACC AAGTTGACAT TGCTACACAA
    GGCTGGTTTA TTGGGCTCAT GTGTGCAGTT GCTCTACTGG TACTGATTTT GCTGATCGTT
    TGTTTTATCA AAAGAAGCCG AGGAGGCAAA TACCCAGTGC GAGAAAAGAA GGAGGTGGCG
    TTGGGACCAG ATGATAACAA AGAGGGGGAT GGCTCATTTG ACTACAGTGA TGAGGACAAC
    AAGCCCCTGC AGGGTAGTCA AACTTCGCTG GATGGAACCA TAAAGCAACA AGAGAGTGAT
    GACAGCCTTG TTGATTATGG AGAAGGAGGA GAGGGGCAGT TTAATGAAGA TGGATCGTTT
    ATAGGGCAGT ATACAGTGAA AAAGGACAAA GATGAGACTG AGGGAAATGA AAGTTCAGAG
    GCCACATCTC CTGTAAATGC TATCTATTCT CTGGCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa03809
    Clone ID Related Accession (Same CDS sequence) XM_004910696.4 , XM_004910696.3
    Accession Version XM_004910696.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4017bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neurofascin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004910696.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGACAGATT TTCTAGAAAG GAGGTTAACG GTGATCCACT ACTACTGTCT CGGAAGCAAC 
    ATGCCATCAT GTTTAGTCCT TCTCCTGCTC TTTCTCCACC TAGTCTGCAC TATTAAAGTT
    CCACTGGACC CAAACATTCA GCGAGACCTA ATACAGCCCC CAACCATCAT CAAGCAGTCA
    GCAAAGAATT ATATTGTAGA TCCCCGTGAT AACATCCTAA TTGAGTGTGA AGCTCAGGGG
    AATCCTTACC CAATGTTTTC ATGGACAAAA AATGGGAAAT TTTACAATGT GGCAAAAGAC
    CCTCGAGTGT CATTCCGTCG ACGATCAGGC ACCCTTGAAA TAGAAATTCA TGGAGGAGGG
    CGGCCAGAGG ACTATGAGGG AGAATACCAA TGCTCTGCCC GCAACCAACA TGGAACAGCT
    TTATCAAATA AGATCTTACT TCAAGTCTCT CGATCCCCTT TGTGGCCCAA AGAAAACCTT
    GACCCGGTGG TGGTAAATGA AGGTGCCTTC TTGATCCTTC AGTGCAATCC CCCACATGGA
    CTTCCTCCTC CAGTCATATT TTGGATGAGC AGCTTGATGG AGCCTATTGT CCAGGACAAG
    AGGGTTTCTC AGGGACTGAA TGGGGACCTT TATTTCTCCA ACGTTCTGCG CCAGGATACT
    CAGACTGATT ATAGCTGCAA TGCACGCTCC CATTTCACAC ACACCATTCA ACAGAAGAAC
    CCATATACTC TAAAAGTGCT TACCACCAGA GGGGTACCTG AGAGAACCCC AACCTTCTTG
    CTTCCCACGG GGACTTCTAG CAGTAAACTG GTGTTACGCG ATGAGGACTT GCTACTGGAG
    TGTATTGCTT CTGGAGTACC AACGCCAGAC ATCATGTGGT ATAAGAAAGG AGGAGAGCTA
    CCCAGCAGGA AGCTGAAGTT TGAAAATTTT AACAAGACTC TACGCATAAT TGGAGTGTCT
    GAAGAGGACT CGGGGGAGTA TTTCTGCCAA GCTTCAAACA AAATGGGCAG CATTCGGCAC
    ACGATTTCTG TTCGGGTTAA GGCTGCTCCA TATTGGCTTG ATGAGCCCAA GAACTTGATT
    CTTGCACCAG GTGAGAACGG ACGTTTGGTA TGTCGTGCCA GTGGCAGCCC CAAACCTACA
    ATCCAATGGT TTATGAATGG AGATGCTACT GAAGTAGGTG CCCCAAATCC CAACTTCGAA
    ATCGCTGGTG ATACCATTAT TTTTAGAGAA GTACAGAGTG GCAACAGCGC TGTATACCAA
    TGCAATGCTT CTAATGAACA TGGTTATCTG TTAGCTAATG CCTTCGTCAG CATTCTGGAT
    GTTCCATCTC GGATGTTGGG CCCTCGTAAT CAGCTGATTC GAGTGATAGA ATACAACAGG
    ACCCAGCTAG ACTGCCCTTT CTTTGGCTCT CCTATACCTA CACTCCGCTG GTTTAAAAAT
    GGACAGGGTA GTAACCTCGA TGGTGGCAAT TACCGGGTGC ATGAAAATGG GACCCTTGAG
    ATTAATACTG TGAGGAAGGA GGACCAAGGC AAATACACAT GTGTTGCTAC CAATATTTTG
    GGCAATGCTG AAAATACTGT GCGGCTGGAG GTCAAAGAGC CAACTCGTAT TGTAAAGGGC
    CCTGAAGATC AGATTGCGAA GAGGGGCTCT TCTGTGCATC TGGACTGTCG AGTGCGCAGT
    GACTCCTCAC TCAGACTCAC TGTTACCTGG ATAAAAGATG ACACTCAGTT GTATATAAAC
    AGCAGAATGA AGAAGGATGA GGATGGACTT ACAATATTCG GTGTGGCAGA AAAGGATCAG
    GGAGCATATA CATGTGTTGC TAAAACTGAA TTAGACCAGG ACTCTTCTAA AGCTTATCTC
    ACGATACAGG AAACTTCCTC ACCTACTAGC CGACTGACCT CCCAGCTTAT AGACAAACCA
    GACCCCCCAC AGGACCTGGA GCTAACTGAA CCATCAGTTA GGAGTGTTCG CCTCACCTGG
    GTTCCAGGGG ATGATAACAA CAGTCCAATT ACAGGTTTCA TTGTACAATT TGAGGAAGAC
    AGGCATCAGC CCGGTACATG GCACAACCTT GCCTCTGTCC CTGCTAATGT GAACTCTGTC
    ATACTGCAGC TTTCTCCTTA TGTCAACTAC CAGTTTCGTG TTCTTGCACT AAATGAACTT
    GGGAGCAGTA TGCCAAGCCC ATCTTCAGAA CGTTTTCAGA CAGCAGGAGC ACCTCCAGAA
    ACTAATCCAG GTGATGTGAA GGGAGCAGGG ACTAGCAAAA ACAATATGGA AATCACATGG
    ACACCACTGA ACTCCACTCA GGCGTATGGT CCAAACCTGC GCTACAACGT GAAGTGGCGC
    CAACGTCACA CAGGAGAACC TTGGAGCAAT ATTACAGTGT GGGACAACAA GCATGTGGTG
    TGGAATACAC CAGTATACAC CCAATATGAG CTAAGAGTTC AGGCGGAAAA TGAATTTGGA
    AAGGCTCCAG AGCCAAGCAC CATGATTGGA TATTCTGGTG AAGACTATCC TAAGGCTGCA
    CCCACTGACA TTAAGCTTCG GGTCATGAAC AGCACCGCCA TCTCTCTTCA GTGGACACGT
    GTGGCACCTG CCTCTATCCA AGGAAGCCTA CTGGAATACA GAGCTTATTT TTGGAGAGAA
    AGTAGTTTGC TGAAGGGTGT CTGGGTCTCA CGAAAGAAGC AACAACAGAG TTTTATGGGG
    GACCGTGTGA GAGGCATCGT ATCTCAACTG ATTCCATACA GCATCTACAA GCTGTACTTG
    GTGGCAGTGA ATGGCCGAGG GGATGGCCCC AAAAGTGATG TTAAGGAATT CACAACCCCA
    GAGGGAGTGC CGAGTGCTCC TAAATACTTT AGAGTATATG AGCCAAACCG GGAAATGATT
    GTTGTTGAAT GGGGTCACCC AGAGCACCCG AACGGAAACT TGACTGCATA CAACCTCCGC
    ATCCAACCAT TTAATGGCAC CAGAACAGGG AAGGCTGTAT TTGAAACTCT TTCACGTAAT
    CAAACCAAAT ATACCCTGCA AAGAACAGAT CCCGTGTCCC GGTATCGGTT TGCTATCAAT
    GCCAGAACGC AGGTTGGTGC AGGGGAAGTC ATCACAGCTG AGTCTCCTGT CAGACTGGAT
    GAAGTTTCTC CCACGTCAGT GTCTTCTAAG TCTCCTGCAC CTGTCACAGA TGCTCCCACA
    GAGGTCCCAA CTATTCCCCC AACCTCATCT GGTGCCACCA CTCTTCTAGC TACAACCAGT
    ACAACCAGTG TCACAACGAC TACTGCTGGT ACAGAAAGTT CGCTGGTCGC CATTACTAGA
    ATTCCTAATA TAGCCACCAC TGGACCATCT CTATGGAACC TAACAGCATT ACCCAACACT
    TACTGGGCAA ACATCACCTG GATGCATAAC TTAGAGCCAG GCTCTGTGTT TATGGTTGAG
    TATACTGACA GTAATAATAC AGTGAAAACT GTTCCTGTTA AGGCTCATGT CCCTTCTATC
    CAACTGTTTG ATCTGGTGCC TGGGATGACC TACAGGGTGC GGGTTTACTC CCAGGAGCAC
    CCGTCAGTCA CAAGCCTATA TGTGACATTT GATACCAGCT CAGCTTACAC CAAGAACCAA
    GTTGACATTG CTACACAAGG CTGGTTTATT GGGCTCATGT GTGCAGTTGC TCTACTGGTA
    CTGATTTTGC TGATCGTTTG TTTTATCAAA AGAAGCCGAG GAGGCAAATA CCCAGTGCGA
    GAAAAGAAGG AGGTGGCGTT GGGACCAGAT GATAACAAAG AGGGGGATGG CTCATTTGAC
    TACAGTGATG AGGACAACAA GCCCCTGCAG GGTAGTCAAA CTTCGCTGGA TGGAACCATA
    AAGCAACAAG AGAGTGATGA CAGCCTTGTT GATTATGGAG AAGGAGGAGA GGGGCAGTTT
    AATGAAGATG GATCGTTTAT AGGGCAGTAT ACAGTGAAAA AGGACAAAGA TGAGACTGAG
    GGAAATGAAA GTTCAGAGGC CACATCTCCT GTAAATGCTA TCTATTCTCT GGCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004910753.1
    CDS554..4570
    Translation

    Target ORF information:

    RefSeq Version XM_004910696.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neurofascin (nfasc), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004910696.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGACAGATT TTCTAGAAAG GAGGTTAACG GTGATCCACT ACTACTGTCT CGGAAGCAAC 
    ATGCCATCAT GTTTAGTCCT TCTCCTGCTC TTTCTCCACC TAGTCTGCAC TATTAAAGTT
    CCACTGGACC CAAACATTCA GCGAGACCTA ATACAGCCCC CAACCATCAT CAAGCAGTCA
    GCAAAGAATT ATATTGTAGA TCCCCGTGAT AACATCCTAA TTGAGTGTGA AGCTCAGGGG
    AATCCTTACC CAATGTTTTC ATGGACAAAA AATGGGAAAT TTTACAATGT GGCAAAAGAC
    CCTCGAGTGT CATTCCGTCG ACGATCAGGC ACCCTTGAAA TAGAAATTCA TGGAGGAGGG
    CGGCCAGAGG ACTATGAGGG AGAATACCAA TGCTCTGCCC GCAACCAACA TGGAACAGCT
    TTATCAAATA AGATCTTACT TCAAGTCTCT CGATCCCCTT TGTGGCCCAA AGAAAACCTT
    GACCCGGTGG TGGTAAATGA AGGTGCCTTC TTGATCCTTC AGTGCAATCC CCCACATGGA
    CTTCCTCCTC CAGTCATATT TTGGATGAGC AGCTTGATGG AGCCTATTGT CCAGGACAAG
    AGGGTTTCTC AGGGACTGAA TGGGGACCTT TATTTCTCCA ACGTTCTGCG CCAGGATACT
    CAGACTGATT ATAGCTGCAA TGCACGCTCC CATTTCACAC ACACCATTCA ACAGAAGAAC
    CCATATACTC TAAAAGTGCT TACCACCAGA GGGGTACCTG AGAGAACCCC AACCTTCTTG
    CTTCCCACGG GGACTTCTAG CAGTAAACTG GTGTTACGCG ATGAGGACTT GCTACTGGAG
    TGTATTGCTT CTGGAGTACC AACGCCAGAC ATCATGTGGT ATAAGAAAGG AGGAGAGCTA
    CCCAGCAGGA AGCTGAAGTT TGAAAATTTT AACAAGACTC TACGCATAAT TGGAGTGTCT
    GAAGAGGACT CGGGGGAGTA TTTCTGCCAA GCTTCAAACA AAATGGGCAG CATTCGGCAC
    ACGATTTCTG TTCGGGTTAA GGCTGCTCCA TATTGGCTTG ATGAGCCCAA GAACTTGATT
    CTTGCACCAG GTGAGAACGG ACGTTTGGTA TGTCGTGCCA GTGGCAGCCC CAAACCTACA
    ATCCAATGGT TTATGAATGG AGATGCTACT GAAGTAGGTG CCCCAAATCC CAACTTCGAA
    ATCGCTGGTG ATACCATTAT TTTTAGAGAA GTACAGAGTG GCAACAGCGC TGTATACCAA
    TGCAATGCTT CTAATGAACA TGGTTATCTG TTAGCTAATG CCTTCGTCAG CATTCTGGAT
    GTTCCATCTC GGATGTTGGG CCCTCGTAAT CAGCTGATTC GAGTGATAGA ATACAACAGG
    ACCCAGCTAG ACTGCCCTTT CTTTGGCTCT CCTATACCTA CACTCCGCTG GTTTAAAAAT
    GGACAGGGTA GTAACCTCGA TGGTGGCAAT TACCGGGTGC ATGAAAATGG GACCCTTGAG
    ATTAATACTG TGAGGAAGGA GGACCAAGGC AAATACACAT GTGTTGCTAC CAATATTTTG
    GGCAATGCTG AAAATACTGT GCGGCTGGAG GTCAAAGAGC CAACTCGTAT TGTAAAGGGC
    CCTGAAGATC AGATTGCGAA GAGGGGCTCT TCTGTGCATC TGGACTGTCG AGTGCGCAGT
    GACTCCTCAC TCAGACTCAC TGTTACCTGG ATAAAAGATG ACACTCAGTT GTATATAAAC
    AGCAGAATGA AGAAGGATGA GGATGGACTT ACAATATTCG GTGTGGCAGA AAAGGATCAG
    GGAGCATATA CATGTGTTGC TAAAACTGAA TTAGACCAGG ACTCTTCTAA AGCTTATCTC
    ACGATACAGG AAACTTCCTC ACCTACTAGC CGACTGACCT CCCAGCTTAT AGACAAACCA
    GACCCCCCAC AGGACCTGGA GCTAACTGAA CCATCAGTTA GGAGTGTTCG CCTCACCTGG
    GTTCCAGGGG ATGATAACAA CAGTCCAATT ACAGGTTTCA TTGTACAATT TGAGGAAGAC
    AGGCATCAGC CCGGTACATG GCACAACCTT GCCTCTGTCC CTGCTAATGT GAACTCTGTC
    ATACTGCAGC TTTCTCCTTA TGTCAACTAC CAGTTTCGTG TTCTTGCACT AAATGAACTT
    GGGAGCAGTA TGCCAAGCCC ATCTTCAGAA CGTTTTCAGA CAGCAGGAGC ACCTCCAGAA
    ACTAATCCAG GTGATGTGAA GGGAGCAGGG ACTAGCAAAA ACAATATGGA AATCACATGG
    ACACCACTGA ACTCCACTCA GGCGTATGGT CCAAACCTGC GCTACAACGT GAAGTGGCGC
    CAACGTCACA CAGGAGAACC TTGGAGCAAT ATTACAGTGT GGGACAACAA GCATGTGGTG
    TGGAATACAC CAGTATACAC CCAATATGAG CTAAGAGTTC AGGCGGAAAA TGAATTTGGA
    AAGGCTCCAG AGCCAAGCAC CATGATTGGA TATTCTGGTG AAGACTATCC TAAGGCTGCA
    CCCACTGACA TTAAGCTTCG GGTCATGAAC AGCACCGCCA TCTCTCTTCA GTGGACACGT
    GTGGCACCTG CCTCTATCCA AGGAAGCCTA CTGGAATACA GAGCTTATTT TTGGAGAGAA
    AGTAGTTTGC TGAAGGGTGT CTGGGTCTCA CGAAAGAAGC AACAACAGAG TTTTATGGGG
    GACCGTGTGA GAGGCATCGT ATCTCAACTG ATTCCATACA GCATCTACAA GCTGTACTTG
    GTGGCAGTGA ATGGCCGAGG GGATGGCCCC AAAAGTGATG TTAAGGAATT CACAACCCCA
    GAGGGAGTGC CGAGTGCTCC TAAATACTTT AGAGTATATG AGCCAAACCG GGAAATGATT
    GTTGTTGAAT GGGGTCACCC AGAGCACCCG AACGGAAACT TGACTGCATA CAACCTCCGC
    ATCCAACCAT TTAATGGCAC CAGAACAGGG AAGGCTGTAT TTGAAACTCT TTCACGTAAT
    CAAACCAAAT ATACCCTGCA AAGAACAGAT CCCGTGTCCC GGTATCGGTT TGCTATCAAT
    GCCAGAACGC AGGTTGGTGC AGGGGAAGTC ATCACAGCTG AGTCTCCTGT CAGACTGGAT
    GAAGTTTCTC CCACGTCAGT GTCTTCTAAG TCTCCTGCAC CTGTCACAGA TGCTCCCACA
    GAGGTCCCAA CTATTCCCCC AACCTCATCT GGTGCCACCA CTCTTCTAGC TACAACCAGT
    ACAACCAGTG TCACAACGAC TACTGCTGGT ACAGAAAGTT CGCTGGTCGC CATTACTAGA
    ATTCCTAATA TAGCCACCAC TGGACCATCT CTATGGAACC TAACAGCATT ACCCAACACT
    TACTGGGCAA ACATCACCTG GATGCATAAC TTAGAGCCAG GCTCTGTGTT TATGGTTGAG
    TATACTGACA GTAATAATAC AGTGAAAACT GTTCCTGTTA AGGCTCATGT CCCTTCTATC
    CAACTGTTTG ATCTGGTGCC TGGGATGACC TACAGGGTGC GGGTTTACTC CCAGGAGCAC
    CCGTCAGTCA CAAGCCTATA TGTGACATTT GATACCAGCT CAGCTTACAC CAAGAACCAA
    GTTGACATTG CTACACAAGG CTGGTTTATT GGGCTCATGT GTGCAGTTGC TCTACTGGTA
    CTGATTTTGC TGATCGTTTG TTTTATCAAA AGAAGCCGAG GAGGCAAATA CCCAGTGCGA
    GAAAAGAAGG AGGTGGCGTT GGGACCAGAT GATAACAAAG AGGGGGATGG CTCATTTGAC
    TACAGTGATG AGGACAACAA GCCCCTGCAG GGTAGTCAAA CTTCGCTGGA TGGAACCATA
    AAGCAACAAG AGAGTGATGA CAGCCTTGTT GATTATGGAG AAGGAGGAGA GGGGCAGTTT
    AATGAAGATG GATCGTTTAT AGGGCAGTAT ACAGTGAAAA AGGACAAAGA TGAGACTGAG
    GGAAATGAAA GTTCAGAGGC CACATCTCCT GTAAATGCTA TCTATTCTCT GGCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa42746
    Clone ID Related Accession (Same CDS sequence) XM_018091565.1
    Accession Version XM_018091565.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3603bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neurofascin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGTCGCTCG CAGGAACAGA TCAGCGTATG TCGCTTGCAG GAACAGATTG GCGTATGTCG 
    CGCAAAGGAA CAGATTGGCG TATGTCGCGC GAAGGAACAG ATTGGCGTAT GTCGCGCGAA
    GGAACAGATC GGCGTATGTT GCGCGAGGGA ACAGATCAGA GCATGTCACT CGCAGCAAAC
    ATTCAGCGAG ACCTAATACA GCCCCCAACC ATCATCAAGC AGTCAGCAAA GAATTATATT
    GTAGATCCCC GTGATAACAT CCTAATTGAG TGTGAAGCTC AGGGGAATCC TTACCCAATG
    TTTTCATGGA CAAAAAATGG GAAATTTTAC AATGTGGCAA AAGACCCTCG AGTGTCATTC
    CGTCGACGAT CAGGCACCCT TGAAATAGAA ATTCATGGAG GAGGGCGGCC AGAGGACTAT
    GAGGGAGAAT ACCAATGCTC TGCCCGCAAC CAACATGGAA CAGCTTTATC AAATAAGATC
    TTACTTCAAG TCTCTCGATC CCCTTTGTGG CCCAAAGAAA ACCTTGACCC GGTGGTGGTA
    AATGAAGGTG CCTTCTTGAT CCTTCAGTGC AATCCCCCAC ATGGACTTCC TCCTCCAGTC
    ATATTTTGGA TGAGCAGCTT GATGGAGCCT ATTGTCCAGG ACAAGAGGGT TTCTCAGGGA
    CTGAATGGGG ACCTTTATTT CTCCAACGTT CTGCGCCAGG ATACTCAGAC TGATTATAGC
    TGCAATGCAC GCTCCCATTT CACACACACC ATTCAACAGA AGAACCCATA TACTCTAAAA
    GTGCTTACCA CCAGAGGGGT ACCTGAGAGA ACCCCAACCT TCTTGCTTCC CACGGGGACT
    TCTAGCAGTA AACTGGTGTT ACGCGATGAG GACTTGCTAC TGGAGTGTAT TGCTTCTGGA
    GTACCAACGC CAGACATCAT GTGGTATAAG AAAGGAGGAG AGCTACCCAG CAGGAAGCTG
    AAGTTTGAAA ATTTTAACAA GACTCTACGC ATAATTGGAG TGTCTGAAGA GGACTCGGGG
    GAGTATTTCT GCCAAGCTTC AAACAAAATG GGCAGCATTC GGCACACGAT TTCTGTTCGG
    GTTAAGGCTG CTCCATATTG GCTTGATGAG CCCAAGAACT TGATTCTTGC ACCAGGTGAG
    AACGGACGTT TGGTATGTCG TGCCAGTGGC AGCCCCAAAC CTACAATCCA ATGGTTTATG
    AATGGAGATG CTACTGAAGT AGGTGCCCCA AATCCCAACT TCGAAATCGC TGGTGATACC
    ATTATTTTTA GAGAAGTACA GAGTGGCAAC AGCGCTGTAT ACCAATGCAA TGCTTCTAAT
    GAACATGGTT ATCTGTTAGC TAATGCCTTC GTCAGCATTC TGGATGTTCC ATCTCGGATG
    TTGGGCCCTC GTAATCAGCT GATTCGAGTG ATAGAATACA ACAGGACCCA GCTAGACTGC
    CCTTTCTTTG GCTCTCCTAT ACCTACACTC CGCTGGTTTA AAAATGGACA GGGTAGTAAC
    CTCGATGGTG GCAATTACCG GGTGCATGAA AATGGGACCC TTGAGATTAA TACTGTGAGG
    AAGGAGGACC AAGGCAAATA CACATGTGTT GCTACCAATA TTTTGGGCAA TGCTGAAAAT
    ACTGTGCGGC TGGAGGTCAA AGAGCCAACT CGTATTGTAA AGGGCCCTGA AGATCAGATT
    GCGAAGAGGG GCTCTTCTGT GCATCTGGAC TGTCGAGTGC GCAGTGACTC CTCACTCAGA
    CTCACTGTTA CCTGGATAAA AGATGACACT CAGTTGTATA TAAACAGCAG AATGAAGAAG
    GATGAGGATG GACTTACAAT ATTCGGTGTG GCAGAAAAGG ATCAGGGAGC ATATACATGT
    GTTGCTAAAA CTGAATTAGA CCAGGACTCT TCTAAAGCTT ATCTCACGAT ACAGGAAACT
    TCCTCACCTA CTAGCCGACT GACCTCCCAG CTTATAGACA AACCAGACCC CCCACAGGAC
    CTGGAGCTAA CTGAACCATC AGTTAGGAGT GTTCGCCTCA CCTGGGTTCC AGGGGATGAT
    AACAACAGTC CAATTACAGG TTTCATTGTA CAATTTGAGG AAGACAGGCA TCAGCCCGGT
    ACATGGCACA ACCTTGCCTC TGTCCCTGCT AATGTGAACT CTGTCATACT GCAGCTTTCT
    CCTTATGTCA ACTACCAGTT TCGTGTTCTT GCACTAAATG AACTTGGGAG CAGTATGCCA
    AGCCCATCTT CAGAACGTTT TCAGACAGCA GGAGCACCTC CAGAAACTAA TCCAGGTGAT
    GTGAAGGGAG CAGGGACTAG CAAAAACAAT ATGGAAATCA CATGGACACC ACTGAACTCC
    ACTCAGGCGT ATGGTCCAAA CCTGCGCTAC AACGTGAAGT GGCGCCAACG TCACACAGGA
    GAACCTTGGA GCAATATTAC AGTGTGGGAC AACAAGCATG TGGTGTGGAA TACACCAGTA
    TACACCCAAT ATGAGCTAAG AGTTCAGGCG GAAAATGAAT TTGGAAAGGC TCCAGAGCCA
    AGCACCATGA TTGGATATTC TGGTGAAGAC TATCCTAAGG CTGCACCCAC TGACATTAAG
    CTTCGGGTCA TGAACAGCAC CGCCATCTCT CTTCAGTGGA CACGTGTGGC ACCTGCCTCT
    ATCCAAGGAA GCCTACTGGA ATACAGAGCT TATTTTTGGA GAGAAAGTAG TTTGCTGAAG
    GGTGTCTGGG TCTCACGAAA GAAGCAACAA CAGAGTTTTA TGGGGGACCG TGTGAGAGGC
    ATCGTATCTC AACTGATTCC ATACAGCATC TACAAGCTGT ACTTGGTGGC AGTGAATGGC
    CGAGGGGATG GCCCCAAAAG TGATGTTAAG GAATTCACAA CCCCAGAGGG AGTGCCGAGT
    GCTCCTAAAT ACTTTAGAGT ATATGAGCCA AACCGGGAAA TGATTGTTGT TGAATGGGGT
    CACCCAGAGC ACCCGAACGG AAACTTGACT GCATACAACC TCCGCATCCA ACCATTTAAT
    GGCACCAGAA CAGGGAAGGC TGTATTTGAA ACTCTTTCAC GTAATCAAAC CAAATATACC
    CTGCAAAGAA CAGATCCCGT GTCCCGGTAT CGGTTTGCTA TCAATGCCAG AACGCAGGTT
    GGTGCAGGGG AAGTCATCAC AGCTGAGTCT CCTGTCAGAC TGGATGAAGC TTACACCAAG
    AACCAAGTTG ACATTGCTAC ACAAGGCTGG TTTATTGGGC TCATGTGTGC AGTTGCTCTA
    CTGGTACTGA TTTTGCTGAT CGTTTGTTTT ATCAAAAGAA GCCGAGGAGG CAAATACCCA
    GTGCGAGAAA AGAAGGAGGT GGCGTTGGGA CCAGATGATA ACAAAGAGGG GGATGGCTCA
    TTTGACTACA GTGATGAGGA CAACAAGCCC CTGCAGGGTA GTCAAACTTC GCTGGATGGA
    ACCATAAAGC AACAAGAGAG TGATGACAGC CTTGTTGATT ATGGAGAAGG AGGAGAGGGG
    CAGTTTAATG AAGATGGATC GTTTATAGGG CAGTATACAG TGAAAAAGGA CAAAGATGAG
    ACTGAGGGAA ATGAAAGTTC AGAGGCCACA TCTCCTGTAA ATGCTATCTA TTCTCTGGCA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947054.1
    CDS250..3852
    Translation

    Target ORF information:

    RefSeq Version XM_018091565.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neurofascin (nfasc), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091565.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGTCGCTCG CAGGAACAGA TCAGCGTATG TCGCTTGCAG GAACAGATTG GCGTATGTCG 
    CGCAAAGGAA CAGATTGGCG TATGTCGCGC GAAGGAACAG ATTGGCGTAT GTCGCGCGAA
    GGAACAGATC GGCGTATGTT GCGCGAGGGA ACAGATCAGA GCATGTCACT CGCAGCAAAC
    ATTCAGCGAG ACCTAATACA GCCCCCAACC ATCATCAAGC AGTCAGCAAA GAATTATATT
    GTAGATCCCC GTGATAACAT CCTAATTGAG TGTGAAGCTC AGGGGAATCC TTACCCAATG
    TTTTCATGGA CAAAAAATGG GAAATTTTAC AATGTGGCAA AAGACCCTCG AGTGTCATTC
    CGTCGACGAT CAGGCACCCT TGAAATAGAA ATTCATGGAG GAGGGCGGCC AGAGGACTAT
    GAGGGAGAAT ACCAATGCTC TGCCCGCAAC CAACATGGAA CAGCTTTATC AAATAAGATC
    TTACTTCAAG TCTCTCGATC CCCTTTGTGG CCCAAAGAAA ACCTTGACCC GGTGGTGGTA
    AATGAAGGTG CCTTCTTGAT CCTTCAGTGC AATCCCCCAC ATGGACTTCC TCCTCCAGTC
    ATATTTTGGA TGAGCAGCTT GATGGAGCCT ATTGTCCAGG ACAAGAGGGT TTCTCAGGGA
    CTGAATGGGG ACCTTTATTT CTCCAACGTT CTGCGCCAGG ATACTCAGAC TGATTATAGC
    TGCAATGCAC GCTCCCATTT CACACACACC ATTCAACAGA AGAACCCATA TACTCTAAAA
    GTGCTTACCA CCAGAGGGGT ACCTGAGAGA ACCCCAACCT TCTTGCTTCC CACGGGGACT
    TCTAGCAGTA AACTGGTGTT ACGCGATGAG GACTTGCTAC TGGAGTGTAT TGCTTCTGGA
    GTACCAACGC CAGACATCAT GTGGTATAAG AAAGGAGGAG AGCTACCCAG CAGGAAGCTG
    AAGTTTGAAA ATTTTAACAA GACTCTACGC ATAATTGGAG TGTCTGAAGA GGACTCGGGG
    GAGTATTTCT GCCAAGCTTC AAACAAAATG GGCAGCATTC GGCACACGAT TTCTGTTCGG
    GTTAAGGCTG CTCCATATTG GCTTGATGAG CCCAAGAACT TGATTCTTGC ACCAGGTGAG
    AACGGACGTT TGGTATGTCG TGCCAGTGGC AGCCCCAAAC CTACAATCCA ATGGTTTATG
    AATGGAGATG CTACTGAAGT AGGTGCCCCA AATCCCAACT TCGAAATCGC TGGTGATACC
    ATTATTTTTA GAGAAGTACA GAGTGGCAAC AGCGCTGTAT ACCAATGCAA TGCTTCTAAT
    GAACATGGTT ATCTGTTAGC TAATGCCTTC GTCAGCATTC TGGATGTTCC ATCTCGGATG
    TTGGGCCCTC GTAATCAGCT GATTCGAGTG ATAGAATACA ACAGGACCCA GCTAGACTGC
    CCTTTCTTTG GCTCTCCTAT ACCTACACTC CGCTGGTTTA AAAATGGACA GGGTAGTAAC
    CTCGATGGTG GCAATTACCG GGTGCATGAA AATGGGACCC TTGAGATTAA TACTGTGAGG
    AAGGAGGACC AAGGCAAATA CACATGTGTT GCTACCAATA TTTTGGGCAA TGCTGAAAAT
    ACTGTGCGGC TGGAGGTCAA AGAGCCAACT CGTATTGTAA AGGGCCCTGA AGATCAGATT
    GCGAAGAGGG GCTCTTCTGT GCATCTGGAC TGTCGAGTGC GCAGTGACTC CTCACTCAGA
    CTCACTGTTA CCTGGATAAA AGATGACACT CAGTTGTATA TAAACAGCAG AATGAAGAAG
    GATGAGGATG GACTTACAAT ATTCGGTGTG GCAGAAAAGG ATCAGGGAGC ATATACATGT
    GTTGCTAAAA CTGAATTAGA CCAGGACTCT TCTAAAGCTT ATCTCACGAT ACAGGAAACT
    TCCTCACCTA CTAGCCGACT GACCTCCCAG CTTATAGACA AACCAGACCC CCCACAGGAC
    CTGGAGCTAA CTGAACCATC AGTTAGGAGT GTTCGCCTCA CCTGGGTTCC AGGGGATGAT
    AACAACAGTC CAATTACAGG TTTCATTGTA CAATTTGAGG AAGACAGGCA TCAGCCCGGT
    ACATGGCACA ACCTTGCCTC TGTCCCTGCT AATGTGAACT CTGTCATACT GCAGCTTTCT
    CCTTATGTCA ACTACCAGTT TCGTGTTCTT GCACTAAATG AACTTGGGAG CAGTATGCCA
    AGCCCATCTT CAGAACGTTT TCAGACAGCA GGAGCACCTC CAGAAACTAA TCCAGGTGAT
    GTGAAGGGAG CAGGGACTAG CAAAAACAAT ATGGAAATCA CATGGACACC ACTGAACTCC
    ACTCAGGCGT ATGGTCCAAA CCTGCGCTAC AACGTGAAGT GGCGCCAACG TCACACAGGA
    GAACCTTGGA GCAATATTAC AGTGTGGGAC AACAAGCATG TGGTGTGGAA TACACCAGTA
    TACACCCAAT ATGAGCTAAG AGTTCAGGCG GAAAATGAAT TTGGAAAGGC TCCAGAGCCA
    AGCACCATGA TTGGATATTC TGGTGAAGAC TATCCTAAGG CTGCACCCAC TGACATTAAG
    CTTCGGGTCA TGAACAGCAC CGCCATCTCT CTTCAGTGGA CACGTGTGGC ACCTGCCTCT
    ATCCAAGGAA GCCTACTGGA ATACAGAGCT TATTTTTGGA GAGAAAGTAG TTTGCTGAAG
    GGTGTCTGGG TCTCACGAAA GAAGCAACAA CAGAGTTTTA TGGGGGACCG TGTGAGAGGC
    ATCGTATCTC AACTGATTCC ATACAGCATC TACAAGCTGT ACTTGGTGGC AGTGAATGGC
    CGAGGGGATG GCCCCAAAAG TGATGTTAAG GAATTCACAA CCCCAGAGGG AGTGCCGAGT
    GCTCCTAAAT ACTTTAGAGT ATATGAGCCA AACCGGGAAA TGATTGTTGT TGAATGGGGT
    CACCCAGAGC ACCCGAACGG AAACTTGACT GCATACAACC TCCGCATCCA ACCATTTAAT
    GGCACCAGAA CAGGGAAGGC TGTATTTGAA ACTCTTTCAC GTAATCAAAC CAAATATACC
    CTGCAAAGAA CAGATCCCGT GTCCCGGTAT CGGTTTGCTA TCAATGCCAG AACGCAGGTT
    GGTGCAGGGG AAGTCATCAC AGCTGAGTCT CCTGTCAGAC TGGATGAAGC TTACACCAAG
    AACCAAGTTG ACATTGCTAC ACAAGGCTGG TTTATTGGGC TCATGTGTGC AGTTGCTCTA
    CTGGTACTGA TTTTGCTGAT CGTTTGTTTT ATCAAAAGAA GCCGAGGAGG CAAATACCCA
    GTGCGAGAAA AGAAGGAGGT GGCGTTGGGA CCAGATGATA ACAAAGAGGG GGATGGCTCA
    TTTGACTACA GTGATGAGGA CAACAAGCCC CTGCAGGGTA GTCAAACTTC GCTGGATGGA
    ACCATAAAGC AACAAGAGAG TGATGACAGC CTTGTTGATT ATGGAGAAGG AGGAGAGGGG
    CAGTTTAATG AAGATGGATC GTTTATAGGG CAGTATACAG TGAAAAAGGA CAAAGATGAG
    ACTGAGGGAA ATGAAAGTTC AGAGGCCACA TCTCCTGTAA ATGCTATCTA TTCTCTGGCA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa42747
    Clone ID Related Accession (Same CDS sequence) XM_018091566.1
    Accession Version XM_018091566.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3282bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neurofascin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTCGCTCG CAGGAACAGA TCAGCGTATG TCGCTTGCAG GAACAGATTG GCGTATGTCG 
    CGCAAAGGAA CAGATTGGCG TATGTCGCGC GAAGGAACAG ATTGGCGTAT GTCGCGCGAA
    GGAACAGATC GGCGTATGTT GCGCGAGGGA ACAGATCAGA GCATGTCACT CGCAGCAAAC
    ATTCAGCGAG ACCTAATACA GCCCCCAACC ATCATCAAGC AGTCAGCAAA GAATTATATT
    GTAGATCCCC GTGATAACAT CCTAATTGAG TGTGAAGCTC AGGGGAATCC TTACCCAATG
    TTTTCATGGA CAAAAAATGG GAAATTTTAC AATGTGGCAA AAGACCCTCG AGTGTCATTC
    CGTCGACGAT CAGGCACCCT TGAAATAGAA ATTCATGGAG GAGGGCGGCC AGAGGACTAT
    GAGGGAGAAT ACCAATGCTC TGCCCGCAAC CAACATGGAA CAGCTTTATC AAATAAGATC
    TTACTTCAAG TCTCTCGATC CCCTTTGTGG CCCAAAGAAA ACCTTGACCC GGTGGTGGTA
    AATGAAGGTG CCTTCTTGAT CCTTCAGTGC AATCCCCCAC ATGGACTTCC TCCTCCAGTC
    ATATTTTGGA TGAGCAGCTT GATGGAGCCT ATTGTCCAGG ACAAGAGGGT TTCTCAGGGA
    CTGAATGGGG ACCTTTATTT CTCCAACGTT CTGCGCCAGG ATACTCAGAC TGATTATAGC
    TGCAATGCAC GCTCCCATTT CACACACACC ATTCAACAGA AGAACCCATA TACTCTAAAA
    GTGCTTACCA CCAGAGGGGT ACCTGAGAGA ACCCCAACCT TCTTGCTTCC CACGGGGACT
    TCTAGCAGTA AACTGGTGTT ACGCGATGAG GACTTGCTAC TGGAGTGTAT TGCTTCTGGA
    GTACCAACGC CAGACATCAT GTGGTATAAG AAAGGAGGAG AGCTACCCAG CAGGAAGCTG
    AAGTTTGAAA ATTTTAACAA GACTCTACGC ATAATTGGAG TGTCTGAAGA GGACTCGGGG
    GAGTATTTCT GCCAAGCTTC AAACAAAATG GGCAGCATTC GGCACACGAT TTCTGTTCGG
    GTTAAGGCTG CTCCATATTG GCTTGATGAG CCCAAGAACT TGATTCTTGC ACCAGGTGAG
    AACGGACGTT TGGTATGTCG TGCCAGTGGC AGCCCCAAAC CTACAATCCA ATGGTTTATG
    AATGGAGATG CTACTGAAGT AGGTGCCCCA AATCCCAACT TCGAAATCGC TGGTGATACC
    ATTATTTTTA GAGAAGTACA GAGTGGCAAC AGCGCTGTAT ACCAATGCAA TGCTTCTAAT
    GAACATGGTT ATCTGTTAGC TAATGCCTTC GTCAGCATTC TGGATGTTCC ATCTCGGATG
    TTGGGCCCTC GTAATCAGCT GATTCGAGTG ATAGAATACA ACAGGACCCA GCTAGACTGC
    CCTTTCTTTG GCTCTCCTAT ACCTACACTC CGCTGGTTTA AAAATGGACA GGGTAGTAAC
    CTCGATGGTG GCAATTACCG GGTGCATGAA AATGGGACCC TTGAGATTAA TACTGTGAGG
    AAGGAGGACC AAGGCAAATA CACATGTGTT GCTACCAATA TTTTGGGCAA TGCTGAAAAT
    ACTGTGCGGC TGGAGGTCAA AGAGCCAACT CGTATTGTAA AGGGCCCTGA AGATCAGATT
    GCGAAGAGGG GCTCTTCTGT GCATCTGGAC TGTCGAGTGC GCAGTGACTC CTCACTCAGA
    CTCACTGTTA CCTGGATAAA AGATGACACT CAGTTGTATA TAAACAGCAG AATGAAGAAG
    GATGAGGATG GACTTACAAT ATTCGGTGTG GCAGAAAAGG ATCAGGGAGC ATATACATGT
    GTTGCTAAAA CTGAATTAGA CCAGGACTCT TCTAAAGCTT ATCTCACGAT ACAGGAAACT
    TCCTCACCTA CTAGCCGACT GACCTCCCAG CTTATAGACA AACCAGACCC CCCACAGGAC
    CTGGAGCTAA CTGAACCATC AGTTAGGAGT GTTCGCCTCA CCTGGGTTCC AGGGGATGAT
    AACAACAGTC CAATTACAGG TTTCATTGTA CAATTTGAGG AAGACAGGCA TCAGCCCGGT
    ACATGGCACA ACCTTGCCTC TGTCCCTGCT AATGTGAACT CTGTCATACT GCAGCTTTCT
    CCTTATGTCA ACTACCAGTT TCGTGTTCTT GCACTAAATG AACTTGGGAG CAGTATGCCA
    AGCCCATCTT CAGAACGTTT TCAGACAGCA GGAGCACCTC CAGAAACTAA TCCAGGTGAT
    GTGAAGGGAG CAGGGACTAG CAAAAACAAT ATGGAAATCA CATGGACACC ACTGAACTCC
    ACTCAGGCGT ATGGTCCAAA CCTGCGCTAC AACGTGAAGT GGCGCCAACG TCACACAGGA
    GAACCTTGGA GCAATATTAC AGTGTGGGAC AACAAGCATG TGGTGTGGAA TACACCAGTA
    TACACCCAAT ATGAGCTAAG AGTTCAGGCG GAAAATGAAT TTGGAAAGGC TCCAGAGCCA
    AGCACCATGA TTGGATATTC TGGTGAAGAC TTGCCGAGTG CTCCTAAATA CTTTAGAGTA
    TATGAGCCAA ACCGGGAAAT GATTGTTGTT GAATGGGGTC ACCCAGAGCA CCCGAACGGA
    AACTTGACTG CATACAACCT CCGCATCCAA CCATTTAATG GCACCAGAAC AGGGAAGGCT
    GTATTTGAAA CTCTTTCACG TAATCAAACC AAATATACCC TGCAAAGAAC AGATCCCGTG
    TCCCGGTATC GGTTTGCTAT CAATGCCAGA ACGCAGGTTG GTGCAGGGGA AGTCATCACA
    GCTGAGTCTC CTGTCAGACT GGATGAAGCT TACACCAAGA ACCAAGTTGA CATTGCTACA
    CAAGGCTGGT TTATTGGGCT CATGTGTGCA GTTGCTCTAC TGGTACTGAT TTTGCTGATC
    GTTTGTTTTA TCAAAAGAAG CCGAGGAGGC AAATACCCAG TGCGAGAAAA GAAGGAGGTG
    GCGTTGGGAC CAGATGATAA CAAAGAGGGG GATGGCTCAT TTGACTACAG TGATGAGGAC
    AACAAGCCCC TGCAGGGTAG TCAAACTTCG CTGGATGGAA CCATAAAGCA ACAAGAGAGT
    GATGACAGCC TTGTTGATTA TGGAGAAGGA GGAGAGGGGC AGTTTAATGA AGATGGATCG
    TTTATAGGGC AGTATACAGT GAAAAAGGAC AAAGATGAGA CTGAGGGAAA TGAAAGTTCA
    GAGGCCACAT CTCCTGTAAA TGCTATCTAT TCTCTGGCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947055.1
    CDS245..3526
    Translation

    Target ORF information:

    RefSeq Version XM_018091566.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neurofascin (nfasc), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091566.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTCGCTCG CAGGAACAGA TCAGCGTATG TCGCTTGCAG GAACAGATTG GCGTATGTCG 
    CGCAAAGGAA CAGATTGGCG TATGTCGCGC GAAGGAACAG ATTGGCGTAT GTCGCGCGAA
    GGAACAGATC GGCGTATGTT GCGCGAGGGA ACAGATCAGA GCATGTCACT CGCAGCAAAC
    ATTCAGCGAG ACCTAATACA GCCCCCAACC ATCATCAAGC AGTCAGCAAA GAATTATATT
    GTAGATCCCC GTGATAACAT CCTAATTGAG TGTGAAGCTC AGGGGAATCC TTACCCAATG
    TTTTCATGGA CAAAAAATGG GAAATTTTAC AATGTGGCAA AAGACCCTCG AGTGTCATTC
    CGTCGACGAT CAGGCACCCT TGAAATAGAA ATTCATGGAG GAGGGCGGCC AGAGGACTAT
    GAGGGAGAAT ACCAATGCTC TGCCCGCAAC CAACATGGAA CAGCTTTATC AAATAAGATC
    TTACTTCAAG TCTCTCGATC CCCTTTGTGG CCCAAAGAAA ACCTTGACCC GGTGGTGGTA
    AATGAAGGTG CCTTCTTGAT CCTTCAGTGC AATCCCCCAC ATGGACTTCC TCCTCCAGTC
    ATATTTTGGA TGAGCAGCTT GATGGAGCCT ATTGTCCAGG ACAAGAGGGT TTCTCAGGGA
    CTGAATGGGG ACCTTTATTT CTCCAACGTT CTGCGCCAGG ATACTCAGAC TGATTATAGC
    TGCAATGCAC GCTCCCATTT CACACACACC ATTCAACAGA AGAACCCATA TACTCTAAAA
    GTGCTTACCA CCAGAGGGGT ACCTGAGAGA ACCCCAACCT TCTTGCTTCC CACGGGGACT
    TCTAGCAGTA AACTGGTGTT ACGCGATGAG GACTTGCTAC TGGAGTGTAT TGCTTCTGGA
    GTACCAACGC CAGACATCAT GTGGTATAAG AAAGGAGGAG AGCTACCCAG CAGGAAGCTG
    AAGTTTGAAA ATTTTAACAA GACTCTACGC ATAATTGGAG TGTCTGAAGA GGACTCGGGG
    GAGTATTTCT GCCAAGCTTC AAACAAAATG GGCAGCATTC GGCACACGAT TTCTGTTCGG
    GTTAAGGCTG CTCCATATTG GCTTGATGAG CCCAAGAACT TGATTCTTGC ACCAGGTGAG
    AACGGACGTT TGGTATGTCG TGCCAGTGGC AGCCCCAAAC CTACAATCCA ATGGTTTATG
    AATGGAGATG CTACTGAAGT AGGTGCCCCA AATCCCAACT TCGAAATCGC TGGTGATACC
    ATTATTTTTA GAGAAGTACA GAGTGGCAAC AGCGCTGTAT ACCAATGCAA TGCTTCTAAT
    GAACATGGTT ATCTGTTAGC TAATGCCTTC GTCAGCATTC TGGATGTTCC ATCTCGGATG
    TTGGGCCCTC GTAATCAGCT GATTCGAGTG ATAGAATACA ACAGGACCCA GCTAGACTGC
    CCTTTCTTTG GCTCTCCTAT ACCTACACTC CGCTGGTTTA AAAATGGACA GGGTAGTAAC
    CTCGATGGTG GCAATTACCG GGTGCATGAA AATGGGACCC TTGAGATTAA TACTGTGAGG
    AAGGAGGACC AAGGCAAATA CACATGTGTT GCTACCAATA TTTTGGGCAA TGCTGAAAAT
    ACTGTGCGGC TGGAGGTCAA AGAGCCAACT CGTATTGTAA AGGGCCCTGA AGATCAGATT
    GCGAAGAGGG GCTCTTCTGT GCATCTGGAC TGTCGAGTGC GCAGTGACTC CTCACTCAGA
    CTCACTGTTA CCTGGATAAA AGATGACACT CAGTTGTATA TAAACAGCAG AATGAAGAAG
    GATGAGGATG GACTTACAAT ATTCGGTGTG GCAGAAAAGG ATCAGGGAGC ATATACATGT
    GTTGCTAAAA CTGAATTAGA CCAGGACTCT TCTAAAGCTT ATCTCACGAT ACAGGAAACT
    TCCTCACCTA CTAGCCGACT GACCTCCCAG CTTATAGACA AACCAGACCC CCCACAGGAC
    CTGGAGCTAA CTGAACCATC AGTTAGGAGT GTTCGCCTCA CCTGGGTTCC AGGGGATGAT
    AACAACAGTC CAATTACAGG TTTCATTGTA CAATTTGAGG AAGACAGGCA TCAGCCCGGT
    ACATGGCACA ACCTTGCCTC TGTCCCTGCT AATGTGAACT CTGTCATACT GCAGCTTTCT
    CCTTATGTCA ACTACCAGTT TCGTGTTCTT GCACTAAATG AACTTGGGAG CAGTATGCCA
    AGCCCATCTT CAGAACGTTT TCAGACAGCA GGAGCACCTC CAGAAACTAA TCCAGGTGAT
    GTGAAGGGAG CAGGGACTAG CAAAAACAAT ATGGAAATCA CATGGACACC ACTGAACTCC
    ACTCAGGCGT ATGGTCCAAA CCTGCGCTAC AACGTGAAGT GGCGCCAACG TCACACAGGA
    GAACCTTGGA GCAATATTAC AGTGTGGGAC AACAAGCATG TGGTGTGGAA TACACCAGTA
    TACACCCAAT ATGAGCTAAG AGTTCAGGCG GAAAATGAAT TTGGAAAGGC TCCAGAGCCA
    AGCACCATGA TTGGATATTC TGGTGAAGAC TTGCCGAGTG CTCCTAAATA CTTTAGAGTA
    TATGAGCCAA ACCGGGAAAT GATTGTTGTT GAATGGGGTC ACCCAGAGCA CCCGAACGGA
    AACTTGACTG CATACAACCT CCGCATCCAA CCATTTAATG GCACCAGAAC AGGGAAGGCT
    GTATTTGAAA CTCTTTCACG TAATCAAACC AAATATACCC TGCAAAGAAC AGATCCCGTG
    TCCCGGTATC GGTTTGCTAT CAATGCCAGA ACGCAGGTTG GTGCAGGGGA AGTCATCACA
    GCTGAGTCTC CTGTCAGACT GGATGAAGCT TACACCAAGA ACCAAGTTGA CATTGCTACA
    CAAGGCTGGT TTATTGGGCT CATGTGTGCA GTTGCTCTAC TGGTACTGAT TTTGCTGATC
    GTTTGTTTTA TCAAAAGAAG CCGAGGAGGC AAATACCCAG TGCGAGAAAA GAAGGAGGTG
    GCGTTGGGAC CAGATGATAA CAAAGAGGGG GATGGCTCAT TTGACTACAG TGATGAGGAC
    AACAAGCCCC TGCAGGGTAG TCAAACTTCG CTGGATGGAA CCATAAAGCA ACAAGAGAGT
    GATGACAGCC TTGTTGATTA TGGAGAAGGA GGAGAGGGGC AGTTTAATGA AGATGGATCG
    TTTATAGGGC AGTATACAGT GAAAAAGGAC AAAGATGAGA CTGAGGGAAA TGAAAGTTCA
    GAGGCCACAT CTCCTGTAAA TGCTATCTAT TCTCTGGCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.