cacna1f cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following cacna1f gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cacna1f cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa33472 XM_012968727.2
Latest version!
Xenopus tropicalis calcium channel, voltage-dependent, L type, alpha 1F subunit (cacna1f), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OXa64765 XM_031891455.1
Latest version!
Xenopus tropicalis calcium channel, voltage-dependent, L type, alpha 1F subunit (cacna1f), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa64766 XM_031891456.1
Latest version!
Xenopus tropicalis calcium channel, voltage-dependent, L type, alpha 1F subunit (cacna1f), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa33472
    Clone ID Related Accession (Same CDS sequence) XM_012968727.2
    Accession Version XM_012968727.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5958bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product voltage-dependent L-type calcium channel subunit alpha-1F
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968727.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGTCAGAAC CAGAGGAGAA TGACAAAGGC CCTGCTCCTC CATACTCCAG CGAGGAGGCT 
    GAGCCGTTCA TGGCAAGTGC AGAGGCAGCG CTGGCCTGTT GGCGATACTC CATGGATGGT
    GGGCCAATGA TGAATGGCAC CGACACCTTA AACTCCACTG CCAGCTCCCA GGCGCAGAAG
    AAACGGCAGT ACCACAGCAA GCACAAGAAC CAGGGAACAG GGGGAGTGCA TAGGAGCCCC
    AGGGCTCTCT TTTGTCTGTA CCTCAGCAAC CCCATTCGAA GGGCCTGCAT CAGCATTGTA
    GAATGGAAAC CATTTGATAT TCTCATTCTC ATGACTATTT TCGCCAACTG CGTGGCGTTG
    GGTGTCTACA TTCCGTTTCC CGAGGACGAT TCCAACATTG CCAATCACAA CCTGGAACAA
    GTGGAGTATA TTTTCCTCAT TATCTTCACC GTGGAGTCCT TCCTAAAGAT AATTGCATAC
    GGGCTGGTCT TGCACCCTAG TGCCTACATC AGAAACGGCT GGAACCTGCT GGACTTTGTC
    ATTGTTGTTA TTGGGTTATT CAGTGTTATT TTGGAACAGT TCTCTCACAA GCAAGGAGAT
    GCCCACCACA TGAGTGGAAA GCCAGGAGGG TTTGATGTGA AAGCTCTGCG TGCATTTCGT
    GTGTTAAGAC CTCTCAGGCT GGTATCTGGA GTCCCAAGCT TGCACATTGT ATTAAATTCC
    ATAATGAAAG CCATGGTTCC CTTATTGCAC ATTGCCTTGC TTGTCCTCTT TGTTATCATC
    ATTTATGCCA TTATTGGGCT GGAGCTCTTC ATTGGTCGGA TGCACAAGAC ATGTTTCTAC
    ATTGGTTCAG ATCTAGTTTC AGAGGAGGAA CCAGCCCCCT GTGCTTTCTC AGGACACGGA
    AGAGAATGTC TCCTCAACAA TACAGAGTGC AGAGGAAAGT GGGAAGGGCC TAACGGGGGA
    ATCACAAACT TTGATAACTT CTTCTTTGCC ATGCTGACTG TGTTCCAATG TATCACTATG
    GAAGGTTGGA CAGATGTTCT CTACTGGATG CAGGATGCCA TGGGTTATGA GCTTCCCTGG
    GTATATTTCG TCAGCCTTGT TATTTTTGGA TCATTCTTTG TTTTGAACCT TGTATTGGGT
    GTGTTGAGTG GAGAATTTTC TAAAGAAAGA GAAAAGGCCA AGGCCCGAGG GGACTTCCAA
    AAGCTGAGGG AGAAACAGCA GATGGAAGAG GATCTCAAGG GATACCTAGA CTGGATCACC
    CAGGCCGAAG ATCTTGAACC TGATCAAGAA ATCAGAGATG AGCGGCACCG CCTGTCTCTG
    GCTGACATCA CTGGCAGGAG GAAGCGGAGA CTTCAATGGC TTTTGCATGC TAACCATTCC
    ACAGAGACCC ACACTAGCCT TCCAGCAAGT GAGACCACTT CTGTGAACAC CGAGAATATA
    GACGAGGAGC ATGCTAACTG CTGTGCCCGC TGTTTGAGCA AGCTGTCACA GACTGGGTGC
    TGCCGTAAGA TAAGGCGGTG GAACCGAGCC TTTCGACGGA AATGCCGCCT GGCTGTTAAA
    TCGGTCACCT TCTACTGGAT GGTTCTGCTC CTCGTCTTCC TCAACACGCT GACCATTGCA
    TCAGAGCATT ACCAACAGCC AGATTGGCTG ACTGAGATTC AAGCGTATGC CAACAAGGTG
    CTTCTGTCTC TATTCACACT GGAGATGCTT CTCAAGATGT ACAGCCTTGG GTTACACGCC
    TACTTTGTGT CCTTCTTTAA CCGCTTTGAT TGCTTCGTGG TTTGTGGTGG GATCTTGGAG
    ACTGTCCTTG TAGAATTTGA CATCATGTCT CCTCTGGGAA TCTCTGTTCT TCGTTGTGTA
    CGACTATTGA GGATCTTTAA GGTCACCAGA CACTGGGCAT CACTAAGTAA TTTGGTGGCT
    TCCCTTTTGA ACTCCATGAA ATCCATTGCT TCTTTGCTGC TGCTTCTCTT CCTCTTCATT
    ATCATCTTCT CCTTGCTCGG AATGCAGCTC TTTGGGGGCA AATTCAACTT TGATGAGACC
    CAGACCAAGA GGAGCACATT TGACACCTTC CCTCAGGCCC TGCTTACTGT CTTCCAGATA
    CTCACAGGTG AGGACTGGAA TGCAGTCATG TACGATGGGA TAATGGCGTA TGGGGGACCA
    TTTTTCCCTG GAATGCTGGT CTGTGTGTAT TTTATCATCC TCTTCATATG TGGTAACTAT
    ATCCTACTCA ATGTCTTTTT GGCCATCGCT GTGGACAATC TGGCTGATGG TGACAACATC
    AATAGCAAAG ATAAAAAGGT GAACAACAAA TCAGAGAAGA AGGATGAAAA CTCTGTCAAG
    GTTCCGTGTG AGGAGGAGAA TGAAAACCAG GAGGAGGAAG GGAGTGAAGC CTCTGAAGAA
    GAGGAAGATG ATGAGGGCAG CCTCCCAGAA TCACACTTGG ATAAAATTGC TGACTTTGCT
    CCAAAGGAGA AGGTTCTTCC CATTCCCGAG GGCAGTGCAT TCTTCATCCT CAGTAACACC
    AACCTCCTCC GCGTTGGGTG CCACAAGCTG ATTCACCACC ACATTTTCAC CAACCTCATT
    TTAGTCTTCA TCATCCTCAG CAGCATTTCC CTGGCAGCCG AAGATCCAAT CCGCGCCCAC
    TCTTTTCGTA ATCATATCCT GGGGTATTTT GATTATGCAT TCACGTCTAT CTTCACAGTG
    GAGATTTTGC TGAAGATGAC AGCATATGGT GCATTCTTGC ACAAGGGCTC CTTCTGCAGG
    AACTGGTTCA ATCTGCTGGA TCTCCTTGTT GTCAGCGTGT CTCTCATCAG TTTTGGAATC
    CATTCTAGCG CTATCTCCGT TGTAAAGATT CTACGTGTTC TGAGAGTACT GCGTCCATTA
    AGAGCCATCA ACAGAGCCAA GGGGCTTAAG CACGTGGTCC AGTGCGTCTT TGTTGCAATC
    CGCACTATCG GTAACATTAT GATAGTGACT ACTTTGCTGC AGTTCATGTT CGCCTGCATA
    GGTGTTCAGC TTTTCAAGGG CAAGTTTTAC AGTTGCACTG ATGAGGCCAA ACGCAGCCCA
    GAGGAGTGCA AGTTACTATA CAAAGCCATT GATGCCTATG GGGAGAACGT AGGACCTATT
    TACAACTACC GTGTGGAGAT CTCCATCTTT TTTATTGTCT ACATTATCAT CATTGCCTTC
    TTCATGATGA ACATCTTTGT GGGTTTTGTG ATTATCACTT TCCGAGCACA AGGAGAGCAG
    GAGTACAAGA ACTGTGAACT GGACAAGAAC CAGCGCCAGT GTGTGGAATA TGCATTGAAG
    GCTCAGCCTC TGCGGCTGTA CATCCCCAAG GCAAAGTATC AGTACAAAGT CTGGTACATG
    GTCAACTCAA CTGGCTTTGA GTACATCATG TTTGTCCTTA TCCTGCTCAA CACTGTTGCT
    TTAGCCATGC AGCATTATGA GCAGTCCAAG CCCTTTAATT ATGCAATGGA TATCCTGAAT
    ATGGTCTTCA CTGGACTCTT CACCGTGGAA ATGGTGCTAA AGCTGATGGC ATTTCGACCC
    AAGCATTATT TCTGCGATGC CTGGAACACA TTTGATGCTC TCATTGTAGT GGGCAGCCTT
    GTGGATATTG CAGTCACCGA GGTCAATAAT GGATGGCATG TTGGTGAGAG TTCAGAGGAT
    AGCTCTCGTA TTTCCATCAC ATTCTTCCGG CTCTTCCGCG TCATGCGTTT GGTAAAGCTT
    CTTAGTAAAG GAGAGGGAAT CAGGACACTG CTGTGGACAT TCATCAAATC CTTCCAGGCT
    TTGCCATACG TTGCTCTGCT CATCGCTATG ATATTTTTTA TCTACGCTGT CATCGGCATG
    CAGACATTTG GGAAAATTGC AATGCAAGAT GCGAGTCAAA TCAACAGAAA TAACAACTTT
    CAGACCTTTC CTCAGGCTGT GCTTTTACTG TTCAGGTGTG CAACCGGGGA GGCCTGGCAA
    GACATCATGT TGGCAAGCCT GCCTGGGAAG CGGTGTGACA GCGAGTCCGA CTTTGGTCCT
    GGGGAGGAAT TTACCTGTGG GAGTAACTTC GCTATTGTGT ATTTCATCAG TTTCTTCATG
    CTCTGCGCCT TCCTTATCAT TAACCTGTTT GTGGCTGTCA TTATGGACAA CTTTGACTAC
    CTCACTAGGG ATTGGTCAAT TCTGGGCCCA CATCATTTAG ATGAGTTTAA GAGGATCTGG
    TCGGAATACG ATCCCGAAGC TAAGGGTCGC ATCAAGCACC TGGATGTTGT GACCTTGCTT
    CGCAGGATCC AGCCTCCACT GGGATTTGGG AAGCTGTGCC CCCATCGTGT AGCATGTAAG
    AGGCTGGTGG CCATGAATAT GCCTTTAAAT GCAGATGGGA CTGTCACCTT TAATGCTACA
    CTTTTTGCCC TTGTTCGAAC ATCACTCAAG ATTAAAACTG AAGGTAACCT AGAGACTGCC
    AATGAGGAAC TGAGAGCTGT CATCAAAAAG ATCTGGAAAA GAACCAAGCA GAAGATACTA
    GATGAGGTGA TTCCCCCTCC TGAAGAGGAG GAGGTGACGG TTGGGAAATT TTATGCCACA
    TTCCTGATCC AGGATTACTT TCGCAAGTTT CGCCGAAGAA AAGAAAAGGG GCTCCTGGGA
    ACAGAGAATT TGCCTTGTAA TTCCACAACA CTGCAGGCAG GGCTGAGGAG CCTACAGGAT
    CTTGGTTCAG AGATACGACG AGTGATATCC ACTGAGCTAG AGGAGGAAGA AGATATCTCT
    GGATTCAGAG CACTGGGGCT TCAGGTTTCA GAAGACAAAT CATTGTGCGA AAACTTAACT
    TTGGTGCAGG CAACTGAAAA TAAGATTTCT GGATCTGTAG TATCTCTTAC TAACTCCATT
    GGAGGAGGAA GAGAAGAGCA GCTACCAATG TCTCGAAGAT CATCACTAAT AAATCGGTCT
    GTAGGAGGAT CTAAGGACAG TTTGTTGGTC CCACCCACAC CACCACCCAA TGTGTCGTCC
    AGCATGAAGA GGAGGCAGAT GACCACACGG AGGAGAAGCT CAGAAGGCAG CGGTATCACT
    CCCACTGTGC ATGAAGAGGA AGAGGAGAGT GCAGCAGAAC ATGAGCTGAG ATCTAGACCT
    CCCTCACCGG GGTACGACCA AAGCAATGAG ATACAGCGCC AGTCTAGTTT GGAGGAGTCT
    CAGCAGCGCT CAGCATCTCC TCAGGAAAGA GTGAAAGTTC TGCCTCCTCC GGCTGCCCCT
    CCATCTAGTT TCAGAGCGAA TGGCACCTTG GAAGGAAGAG CAACCCAAAG AAGGCGCCTG
    CTGCCTCCTA CCCCAGGTAC CAAGTCTGCA TTTAGCATCC AGTGCCTGAA GAGACAGGGG
    AGCTGTGATG ATATACCCAT CCCAGGAACA TACCATCAGA ATGCACCCCC ATGCAGAACA
    AGAGGCCAGG TTTATGGAAG TACCGATTCT TGGCAAAGAA ATAGTTACTC CACCAATGGC
    ACTCACTCAT GGGCCAACCC ACCAAAGCGT GGGCGCCTTC TTTATGCACC CCTTATATTG
    GTGGAGGAAG ACAGCGCCCT GCCTACTATT CCATGCACTC GCTGGTATCA GGAAGAGGAT
    AGCAAAGCAC CCTATTGCCA CTACACCCAG CTGCGTGTGC CGGGCCAGAG GCTTGCCGAG
    AAGAGAGGCA GCGCTGACAG TCTAGTGGAG GCGGTTTTGA TATCAGAAGG CCTAGGGCTT
    TATGCACGGG ACCCCAAGTT TGTCGCTTTC GCCAAACGTG AGATCGCAGA TGCCTGTCAG
    ATGACCATTG ATGAGATGGA GAGTGCCGCC AGTGACTTGC TAAGCCAGAG CAGGAAAGGC
    AGTTGGACTG GAAATAATGG ACACTCCTAC CAAGATGAAA CGGCATGGAA TGGACTTTTG
    TACAGTGATG AAGACTCTAT ACACTGCCGA GAAGAAGAGG AATTAAGAGA TGAGATGGCC
    TGCGTGTCTT CATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012824181.1
    CDS286..6243
    Translation

    Target ORF information:

    RefSeq Version XM_012968727.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calcium channel, voltage-dependent, L type, alpha 1F subunit (cacna1f), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012968727.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGTCAGAAC CAGAGGAGAA TGACAAAGGC CCTGCTCCTC CATACTCCAG CGAGGAGGCT 
    GAGCCGTTCA TGGCAAGTGC AGAGGCAGCG CTGGCCTGTT GGCGATACTC CATGGATGGT
    GGGCCAATGA TGAATGGCAC CGACACCTTA AACTCCACTG CCAGCTCCCA GGCGCAGAAG
    AAACGGCAGT ACCACAGCAA GCACAAGAAC CAGGGAACAG GGGGAGTGCA TAGGAGCCCC
    AGGGCTCTCT TTTGTCTGTA CCTCAGCAAC CCCATTCGAA GGGCCTGCAT CAGCATTGTA
    GAATGGAAAC CATTTGATAT TCTCATTCTC ATGACTATTT TCGCCAACTG CGTGGCGTTG
    GGTGTCTACA TTCCGTTTCC CGAGGACGAT TCCAACATTG CCAATCACAA CCTGGAACAA
    GTGGAGTATA TTTTCCTCAT TATCTTCACC GTGGAGTCCT TCCTAAAGAT AATTGCATAC
    GGGCTGGTCT TGCACCCTAG TGCCTACATC AGAAACGGCT GGAACCTGCT GGACTTTGTC
    ATTGTTGTTA TTGGGTTATT CAGTGTTATT TTGGAACAGT TCTCTCACAA GCAAGGAGAT
    GCCCACCACA TGAGTGGAAA GCCAGGAGGG TTTGATGTGA AAGCTCTGCG TGCATTTCGT
    GTGTTAAGAC CTCTCAGGCT GGTATCTGGA GTCCCAAGCT TGCACATTGT ATTAAATTCC
    ATAATGAAAG CCATGGTTCC CTTATTGCAC ATTGCCTTGC TTGTCCTCTT TGTTATCATC
    ATTTATGCCA TTATTGGGCT GGAGCTCTTC ATTGGTCGGA TGCACAAGAC ATGTTTCTAC
    ATTGGTTCAG ATCTAGTTTC AGAGGAGGAA CCAGCCCCCT GTGCTTTCTC AGGACACGGA
    AGAGAATGTC TCCTCAACAA TACAGAGTGC AGAGGAAAGT GGGAAGGGCC TAACGGGGGA
    ATCACAAACT TTGATAACTT CTTCTTTGCC ATGCTGACTG TGTTCCAATG TATCACTATG
    GAAGGTTGGA CAGATGTTCT CTACTGGATG CAGGATGCCA TGGGTTATGA GCTTCCCTGG
    GTATATTTCG TCAGCCTTGT TATTTTTGGA TCATTCTTTG TTTTGAACCT TGTATTGGGT
    GTGTTGAGTG GAGAATTTTC TAAAGAAAGA GAAAAGGCCA AGGCCCGAGG GGACTTCCAA
    AAGCTGAGGG AGAAACAGCA GATGGAAGAG GATCTCAAGG GATACCTAGA CTGGATCACC
    CAGGCCGAAG ATCTTGAACC TGATCAAGAA ATCAGAGATG AGCGGCACCG CCTGTCTCTG
    GCTGACATCA CTGGCAGGAG GAAGCGGAGA CTTCAATGGC TTTTGCATGC TAACCATTCC
    ACAGAGACCC ACACTAGCCT TCCAGCAAGT GAGACCACTT CTGTGAACAC CGAGAATATA
    GACGAGGAGC ATGCTAACTG CTGTGCCCGC TGTTTGAGCA AGCTGTCACA GACTGGGTGC
    TGCCGTAAGA TAAGGCGGTG GAACCGAGCC TTTCGACGGA AATGCCGCCT GGCTGTTAAA
    TCGGTCACCT TCTACTGGAT GGTTCTGCTC CTCGTCTTCC TCAACACGCT GACCATTGCA
    TCAGAGCATT ACCAACAGCC AGATTGGCTG ACTGAGATTC AAGCGTATGC CAACAAGGTG
    CTTCTGTCTC TATTCACACT GGAGATGCTT CTCAAGATGT ACAGCCTTGG GTTACACGCC
    TACTTTGTGT CCTTCTTTAA CCGCTTTGAT TGCTTCGTGG TTTGTGGTGG GATCTTGGAG
    ACTGTCCTTG TAGAATTTGA CATCATGTCT CCTCTGGGAA TCTCTGTTCT TCGTTGTGTA
    CGACTATTGA GGATCTTTAA GGTCACCAGA CACTGGGCAT CACTAAGTAA TTTGGTGGCT
    TCCCTTTTGA ACTCCATGAA ATCCATTGCT TCTTTGCTGC TGCTTCTCTT CCTCTTCATT
    ATCATCTTCT CCTTGCTCGG AATGCAGCTC TTTGGGGGCA AATTCAACTT TGATGAGACC
    CAGACCAAGA GGAGCACATT TGACACCTTC CCTCAGGCCC TGCTTACTGT CTTCCAGATA
    CTCACAGGTG AGGACTGGAA TGCAGTCATG TACGATGGGA TAATGGCGTA TGGGGGACCA
    TTTTTCCCTG GAATGCTGGT CTGTGTGTAT TTTATCATCC TCTTCATATG TGGTAACTAT
    ATCCTACTCA ATGTCTTTTT GGCCATCGCT GTGGACAATC TGGCTGATGG TGACAACATC
    AATAGCAAAG ATAAAAAGGT GAACAACAAA TCAGAGAAGA AGGATGAAAA CTCTGTCAAG
    GTTCCGTGTG AGGAGGAGAA TGAAAACCAG GAGGAGGAAG GGAGTGAAGC CTCTGAAGAA
    GAGGAAGATG ATGAGGGCAG CCTCCCAGAA TCACACTTGG ATAAAATTGC TGACTTTGCT
    CCAAAGGAGA AGGTTCTTCC CATTCCCGAG GGCAGTGCAT TCTTCATCCT CAGTAACACC
    AACCTCCTCC GCGTTGGGTG CCACAAGCTG ATTCACCACC ACATTTTCAC CAACCTCATT
    TTAGTCTTCA TCATCCTCAG CAGCATTTCC CTGGCAGCCG AAGATCCAAT CCGCGCCCAC
    TCTTTTCGTA ATCATATCCT GGGGTATTTT GATTATGCAT TCACGTCTAT CTTCACAGTG
    GAGATTTTGC TGAAGATGAC AGCATATGGT GCATTCTTGC ACAAGGGCTC CTTCTGCAGG
    AACTGGTTCA ATCTGCTGGA TCTCCTTGTT GTCAGCGTGT CTCTCATCAG TTTTGGAATC
    CATTCTAGCG CTATCTCCGT TGTAAAGATT CTACGTGTTC TGAGAGTACT GCGTCCATTA
    AGAGCCATCA ACAGAGCCAA GGGGCTTAAG CACGTGGTCC AGTGCGTCTT TGTTGCAATC
    CGCACTATCG GTAACATTAT GATAGTGACT ACTTTGCTGC AGTTCATGTT CGCCTGCATA
    GGTGTTCAGC TTTTCAAGGG CAAGTTTTAC AGTTGCACTG ATGAGGCCAA ACGCAGCCCA
    GAGGAGTGCA AGTTACTATA CAAAGCCATT GATGCCTATG GGGAGAACGT AGGACCTATT
    TACAACTACC GTGTGGAGAT CTCCATCTTT TTTATTGTCT ACATTATCAT CATTGCCTTC
    TTCATGATGA ACATCTTTGT GGGTTTTGTG ATTATCACTT TCCGAGCACA AGGAGAGCAG
    GAGTACAAGA ACTGTGAACT GGACAAGAAC CAGCGCCAGT GTGTGGAATA TGCATTGAAG
    GCTCAGCCTC TGCGGCTGTA CATCCCCAAG GCAAAGTATC AGTACAAAGT CTGGTACATG
    GTCAACTCAA CTGGCTTTGA GTACATCATG TTTGTCCTTA TCCTGCTCAA CACTGTTGCT
    TTAGCCATGC AGCATTATGA GCAGTCCAAG CCCTTTAATT ATGCAATGGA TATCCTGAAT
    ATGGTCTTCA CTGGACTCTT CACCGTGGAA ATGGTGCTAA AGCTGATGGC ATTTCGACCC
    AAGCATTATT TCTGCGATGC CTGGAACACA TTTGATGCTC TCATTGTAGT GGGCAGCCTT
    GTGGATATTG CAGTCACCGA GGTCAATAAT GGATGGCATG TTGGTGAGAG TTCAGAGGAT
    AGCTCTCGTA TTTCCATCAC ATTCTTCCGG CTCTTCCGCG TCATGCGTTT GGTAAAGCTT
    CTTAGTAAAG GAGAGGGAAT CAGGACACTG CTGTGGACAT TCATCAAATC CTTCCAGGCT
    TTGCCATACG TTGCTCTGCT CATCGCTATG ATATTTTTTA TCTACGCTGT CATCGGCATG
    CAGACATTTG GGAAAATTGC AATGCAAGAT GCGAGTCAAA TCAACAGAAA TAACAACTTT
    CAGACCTTTC CTCAGGCTGT GCTTTTACTG TTCAGGTGTG CAACCGGGGA GGCCTGGCAA
    GACATCATGT TGGCAAGCCT GCCTGGGAAG CGGTGTGACA GCGAGTCCGA CTTTGGTCCT
    GGGGAGGAAT TTACCTGTGG GAGTAACTTC GCTATTGTGT ATTTCATCAG TTTCTTCATG
    CTCTGCGCCT TCCTTATCAT TAACCTGTTT GTGGCTGTCA TTATGGACAA CTTTGACTAC
    CTCACTAGGG ATTGGTCAAT TCTGGGCCCA CATCATTTAG ATGAGTTTAA GAGGATCTGG
    TCGGAATACG ATCCCGAAGC TAAGGGTCGC ATCAAGCACC TGGATGTTGT GACCTTGCTT
    CGCAGGATCC AGCCTCCACT GGGATTTGGG AAGCTGTGCC CCCATCGTGT AGCATGTAAG
    AGGCTGGTGG CCATGAATAT GCCTTTAAAT GCAGATGGGA CTGTCACCTT TAATGCTACA
    CTTTTTGCCC TTGTTCGAAC ATCACTCAAG ATTAAAACTG AAGGTAACCT AGAGACTGCC
    AATGAGGAAC TGAGAGCTGT CATCAAAAAG ATCTGGAAAA GAACCAAGCA GAAGATACTA
    GATGAGGTGA TTCCCCCTCC TGAAGAGGAG GAGGTGACGG TTGGGAAATT TTATGCCACA
    TTCCTGATCC AGGATTACTT TCGCAAGTTT CGCCGAAGAA AAGAAAAGGG GCTCCTGGGA
    ACAGAGAATT TGCCTTGTAA TTCCACAACA CTGCAGGCAG GGCTGAGGAG CCTACAGGAT
    CTTGGTTCAG AGATACGACG AGTGATATCC ACTGAGCTAG AGGAGGAAGA AGATATCTCT
    GGATTCAGAG CACTGGGGCT TCAGGTTTCA GAAGACAAAT CATTGTGCGA AAACTTAACT
    TTGGTGCAGG CAACTGAAAA TAAGATTTCT GGATCTGTAG TATCTCTTAC TAACTCCATT
    GGAGGAGGAA GAGAAGAGCA GCTACCAATG TCTCGAAGAT CATCACTAAT AAATCGGTCT
    GTAGGAGGAT CTAAGGACAG TTTGTTGGTC CCACCCACAC CACCACCCAA TGTGTCGTCC
    AGCATGAAGA GGAGGCAGAT GACCACACGG AGGAGAAGCT CAGAAGGCAG CGGTATCACT
    CCCACTGTGC ATGAAGAGGA AGAGGAGAGT GCAGCAGAAC ATGAGCTGAG ATCTAGACCT
    CCCTCACCGG GGTACGACCA AAGCAATGAG ATACAGCGCC AGTCTAGTTT GGAGGAGTCT
    CAGCAGCGCT CAGCATCTCC TCAGGAAAGA GTGAAAGTTC TGCCTCCTCC GGCTGCCCCT
    CCATCTAGTT TCAGAGCGAA TGGCACCTTG GAAGGAAGAG CAACCCAAAG AAGGCGCCTG
    CTGCCTCCTA CCCCAGGTAC CAAGTCTGCA TTTAGCATCC AGTGCCTGAA GAGACAGGGG
    AGCTGTGATG ATATACCCAT CCCAGGAACA TACCATCAGA ATGCACCCCC ATGCAGAACA
    AGAGGCCAGG TTTATGGAAG TACCGATTCT TGGCAAAGAA ATAGTTACTC CACCAATGGC
    ACTCACTCAT GGGCCAACCC ACCAAAGCGT GGGCGCCTTC TTTATGCACC CCTTATATTG
    GTGGAGGAAG ACAGCGCCCT GCCTACTATT CCATGCACTC GCTGGTATCA GGAAGAGGAT
    AGCAAAGCAC CCTATTGCCA CTACACCCAG CTGCGTGTGC CGGGCCAGAG GCTTGCCGAG
    AAGAGAGGCA GCGCTGACAG TCTAGTGGAG GCGGTTTTGA TATCAGAAGG CCTAGGGCTT
    TATGCACGGG ACCCCAAGTT TGTCGCTTTC GCCAAACGTG AGATCGCAGA TGCCTGTCAG
    ATGACCATTG ATGAGATGGA GAGTGCCGCC AGTGACTTGC TAAGCCAGAG CAGGAAAGGC
    AGTTGGACTG GAAATAATGG ACACTCCTAC CAAGATGAAA CGGCATGGAA TGGACTTTTG
    TACAGTGATG AAGACTCTAT ACACTGCCGA GAAGAAGAGG AATTAAGAGA TGAGATGGCC
    TGCGTGTCTT CATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa64765
    Clone ID Related Accession (Same CDS sequence) XM_031891455.1
    Accession Version XM_031891455.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6105bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product voltage-dependent L-type calcium channel subunit alpha-1F isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGTCAGAAC CAGAGGAGAA TGACAAAGGC CCTGCTCCTC CATACTCCAG CGAGGAGGCT 
    GAGCCGTTCA TGGCAAGTGC AGAGGCAGCG CTGGCCTGTT GGCGATACTC CATGGATGGT
    GGGCCAATGA TGAATGGCAC CGACACCTTA AACTCCACTG CCAGCTCCCA GGCGCAGAAG
    AAACGGCAGT ACCACAGCAA GCACAAGAAC CAGGGAACAG GGGGAGTGCA TAGGAGCCCC
    AGGGCTCTCT TTTGTCTGTA CCTCAGCAAC CCCATTCGAA GGGCCTGCAT CAGCATTGTA
    GAATGGAAAC CATTTGATAT TCTCATTCTC ATGACTATTT TCGCCAACTG CGTGGCGTTG
    GGTGTCTACA TTCCGTTTCC CGAGGACGAT TCCAACATTG CCAATCACAA CCTGGAACAA
    GTGGAGTATA TTTTCCTCAT TATCTTCACC GTGGAGTCCT TCCTAAAGAT AATTGCATAC
    GGGCTGGTCT TGCACCCTAG TGCCTACATC AGAAACGGCT GGAACCTGCT GGACTTTGTC
    ATTGTTGTTA TTGGGTTATT CAGTGTTATT TTGGAACAGT TCTCTCACAA GCAAGGAGAT
    GCCCACCACA TGAGTGGAAA GCCAGGAGGG TTTGATGTGA AAGCTCTGCG TGCATTTCGT
    GTGTTAAGAC CTCTCAGGCT GGTATCTGGA GTCCCAAGCT TGCACATTGT ATTAAATTCC
    ATAATGAAAG CCATGGTTCC CTTATTGCAC ATTGCCTTGC TTGTCCTCTT TGTTATCATC
    ATTTATGCCA TTATTGGGCT GGAGCTCTTC ATTGGTCGGA TGCACAAGAC ATGTTTCTAC
    ATTGGTTCAG ATCTAGTTTC AGAGGAGGAA CCAGCCCCCT GTGCTTTCTC AGGACACGGA
    AGAGAATGTC TCCTCAACAA TACAGAGTGC AGAGGAAAGT GGGAAGGGCC TAACGGGGGA
    ATCACAAACT TTGATAACTT CTTCTTTGCC ATGCTGACTG TGTTCCAATG TATCACTATG
    GAAGGTTGGA CAGATGTTCT CTACTGGATG CAGGATGCCA TGGGTTATGA GCTTCCCTGG
    GTATATTTCG TCAGCCTTGT TATTTTTGGA TCATTCTTTG TTTTGAACCT TGTATTGGGT
    GTGTTGAGTG GAGAATTTTC TAAAGAAAGA GAAAAGGCCA AGGCCCGAGG GGACTTCCAA
    AAGCTGAGGG AGAAACAGCA GATGGAAGAG GATCTCAAGG GATACCTAGA CTGGATCACC
    CAGGCCGAAG ATCTTGAACC TGATCAAGAA ATCAGAGATG AGCGGCACCG CCTGTCTCTG
    GCTGACATCA CTGGCAGGAG GAAGCGGAGA CTTCAATGGC TTTTGCATGC TAACCATTCC
    ACAGAGACCC ACACTAGCCT TCCAGCAAGT GAGACCACTT CTGTGAACAC CGAGAATATA
    GACGAGGAGC ATGCTAACTG CTGTGCCCGC TGTTTGAGCA AGCTGTCACA GACTGGGTGC
    TGCCGTAAGA TAAGGCGGTG GAACCGAGCC TTTCGACGGA AATGCCGCCT GGCTGTTAAA
    TCGGTCACCT TCTACTGGAT GGTTCTGCTC CTCGTCTTCC TCAACACGCT GACCATTGCA
    TCAGAGCATT ACCAACAGCC AGATTGGCTG ACTGAGATTC AAGCGTATGC CAACAAGGTG
    CTTCTGTCTC TATTCACACT GGAGATGCTT CTCAAGATGT ACAGCCTTGG GTTACACGCC
    TACTTTGTGT CCTTCTTTAA CCGCTTTGAT TGCTTCGTGG TTTGTGGTGG GATCTTGGAG
    ACTGTCCTTG TAGAATTTGA CATCATGTCT CCTCTGGGAA TCTCTGTTCT TCGTTGTGTA
    CGACTATTGA GGATCTTTAA GGTCACCAGA CACTGGGCAT CACTAAGTAA TTTGGTGGCT
    TCCCTTTTGA ACTCCATGAA ATCCATTGCT TCTTTGCTGC TGCTTCTCTT CCTCTTCATT
    ATCATCTTCT CCTTGCTCGG AATGCAGCTC TTTGGGGGCA AATTCAACTT TGATGAGACC
    CAGACCAAGA GGAGCACATT TGACACCTTC CCTCAGGCCC TGCTTACTGT CTTCCAGATA
    CTCACAGGTG AGGACTGGAA TGCAGTCATG TACGATGGGA TAATGGCGTA TGGGGGACCA
    TTTTTCCCTG GAATGCTGGT CTGTGTGTAT TTTATCATCC TCTTCATATG TGGTAACTAT
    ATCCTACTCA ATGTCTTTTT GGCCATCGCT GTGGACAATC TGGCTGATGG TGACAACATC
    AATAGCAAAG ATAAAAAGGT GAACAACAAA TCAGAGAAGA AGGATGAAAA CTCTGTCAAG
    GTTCCGTGTG AGGAGGAGAA TGAAAACCAG GAGGAGGAAG GGAGTGAAGC CTCTGAAGAA
    GAGGAAGATG ATGAGGGCAG CCTCCCAGAA TCACACTTGG ATAAAATTGC TGACTTTGCT
    CCAAAGGAGA AGGTTCTTCC CATTCCCGAG GGCAGTGCAT TCTTCATCCT CAGTAACACC
    AACCTCCTCC GCGTTGGGTG CCACAAGCTG ATTCACCACC ACATTTTCAC CAACCTCATT
    TTAGTCTTCA TCATCCTCAG CAGCATTTCC CTGGCAGCCG AAGATCCAAT CCGCGCCCAC
    TCTTTTCGTA ATCATATCCT GGGGTATTTT GATTATGCAT TCACGTCTAT CTTCACAGTG
    GAGATTTTGC TGAAGATGAC AGCATATGGT GCATTCTTGC ACAAGGGCTC CTTCTGCAGG
    AACTGGTTCA ATCTGCTGGA TCTCCTTGTT GTCAGCGTGT CTCTCATCAG TTTTGGAATC
    CATTCTAGCG CTATCTCCGT TGTAAAGATT CTACGTGTTC TGAGAGTACT GCGTCCATTA
    AGAGCCATCA ACAGAGCCAA GGGGCTTAAG CACGTGGTCC AGTGCGTCTT TGTTGCAATC
    CGCACTATCG GTAACATTAT GATAGTGACT ACTTTGCTGC AGTTCATGTT CGCCTGCATA
    GGTGTTCAGC TTTTCAAGGG CAAGTTTTAC AGTTGCACTG ATGAGGCCAA ACGCAGCCCA
    GAGGAGTGCA AGGGCACATT CATTATTTAT AAGGAAGGGG ACGTTTCACA CCCCATGGTG
    CGGGAAAGAA TTTGGCACAA CAGTGACTTC AACTTTGACA ACGTCCTGTC AGGCATGATG
    GCTCTGTTTA CAGTGTCCAC ATTTGAAGGT TGGCCAGCGT TACTATACAA AGCCATTGAT
    GCCTATGGGG AGAACGTAGG ACCTATTTAC AACTACCGTG TGGAGATCTC CATCTTTTTT
    ATTGTCTACA TTATCATCAT TGCCTTCTTC ATGATGAACA TCTTTGTGGG TTTTGTGATT
    ATCACTTTCC GAGCACAAGG AGAGCAGGAG TACAAGAACT GTGAACTGGA CAAGAACCAG
    CGCCAGTGTG TGGAATATGC ATTGAAGGCT CAGCCTCTGC GGCTGTACAT CCCCAAGGCA
    AAGTATCAGT ACAAAGTCTG GTACATGGTC AACTCAACTG GCTTTGAGTA CATCATGTTT
    GTCCTTATCC TGCTCAACAC TGTTGCTTTA GCCATGCAGC ATTATGAGCA GTCCAAGCCC
    TTTAATTATG CAATGGATAT CCTGAATATG GTCTTCACTG GACTCTTCAC CGTGGAAATG
    GTGCTAAAGC TGATGGCATT TCGACCCAAG CATTATTTCT GCGATGCCTG GAACACATTT
    GATGCTCTCA TTGTAGTGGG CAGCCTTGTG GATATTGCAG TCACCGAGGT CAATAATGGA
    TGGCATGTTG GTGAGAGTTC AGAGGATAGC TCTCGTATTT CCATCACATT CTTCCGGCTC
    TTCCGCGTCA TGCGTTTGGT AAAGCTTCTT AGTAAAGGAG AGGGAATCAG GACACTGCTG
    TGGACATTCA TCAAATCCTT CCAGGCTTTG CCATACGTTG CTCTGCTCAT CGCTATGATA
    TTTTTTATCT ACGCTGTCAT CGGCATGCAG ACATTTGGGA AAATTGCAAT GCAAGATGCG
    AGTCAAATCA ACAGAAATAA CAACTTTCAG ACCTTTCCTC AGGCTGTGCT TTTACTGTTC
    AGGTGTGCAA CCGGGGAGGC CTGGCAAGAC ATCATGTTGG CAAGCCTGCC TGGGAAGCGG
    TGTGACAGCG AGTCCGACTT TGGTCCTGGG GAGGAATTTA CCTGTGGGAG TAACTTCGCT
    ATTGTGTATT TCATCAGTTT CTTCATGCTC TGCGCCTTCC TTATCATTAA CCTGTTTGTG
    GCTGTCATTA TGGACAACTT TGACTACCTC ACTAGGGATT GGTCAATTCT GGGCCCACAT
    CATTTAGATG AGTTTAAGAG GATCTGGTCG GAATACGATC CCGAAGCTAA GGGTCGCATC
    AAGCACCTGG ATGTTGTGAC CTTGCTTCGC AGGATCCAGC CTCCACTGGG ATTTGGGAAG
    CTGTGCCCCC ATCGTGTAGC ATGTAAGAGG CTGGTGGCCA TGAATATGCC TTTAAATGCA
    GATGGGACTG TCACCTTTAA TGCTACACTT TTTGCCCTTG TTCGAACATC ACTCAAGATT
    AAAACTGAAG GTAACCTAGA GACTGCCAAT GAGGAACTGA GAGCTGTCAT CAAAAAGATC
    TGGAAAAGAA CCAAGCAGAA GATACTAGAT GAGGTGATTC CCCCTCCTGA AGAGGAGGAG
    GTGACGGTTG GGAAATTTTA TGCCACATTC CTGATCCAGG ATTACTTTCG CAAGTTTCGC
    CGAAGAAAAG AAAAGGGGCT CCTGGGAACA GAGAATTTGC CTTGTAATTC CACAACACTG
    CAGGCAGGGC TGAGGAGCCT ACAGGATCTT GGTTCAGAGA TACGACGAGT GATATCCACT
    GAGCTAGAGG AGGAAGAAGA TATCTCTGGA TTCAGAGCAC TGGGGCTTCA GGTTTCAGAA
    GACAAATCAT TGTGCGAAAA CTTAACTTTG GTGCAGGCAA CTGAAAATAA GATTTCTGGA
    TCTGTAGTAT CTCTTACTAA CTCCATTGGA GGAGGAAGAG AAGAGCAGCT ACCAATGTCT
    CGAAGATCAT CACTAATAAA TCGGTCTGTA GGAGGATCTA AGGACAGTTT GTTGGTCCCA
    CCCACACCAC CACCCAATGT GTCGTCCAGC ATGAAGAGGA GGCAGATGAC CACACGGAGG
    AGAAGCTCAG AAGGCAGCGG TATCACTCCC ACTGTGCATG AAGAGGAAGA GGAGAGTGCA
    GCAGAACATG AGCTGAGATC TAGACCTCCC TCACCGGGGT ACGACCAAAG CAATGAGATA
    CAGCGCCAGT CTAGTTTGGA GGAGTCTCAG CAGCGCTCAG CATCTCCTCA GGAAAGAGTG
    AAAGTTCTGC CTCCTCCGGC TGCCCCTCCA TCTAGTTTCA GAGCGAATGG CACCTTGGAA
    GGAAGAGCAA CCCAAAGAAG GCGCCTGCTG CCTCCTACCC CAGGTACCAA GTCTGCATTT
    AGCATCCAGT GCCTGAAGAG ACAGGGGAGC TGTGATGATA TACCCATCCC AGGAACATAC
    CATCAGAATG CACCCCCATG CAGAACAAGA GGCCAGGTTT ATGGAAGTAC CGATTCTTGG
    CAAAGAAATA GTTACTCCAC CAATGGCACT CACTCATGGG CCAACCCACC AAAGCGTGGG
    CGCCTTCTTT ATGCACCCCT TATATTGGTG GAGGAAGACA GCGCCCTGCC TACTATTCCA
    TGCACTCGCT GGTATCAGGA AGAGGATAGC AAAGCACCCT ATTGCCACTA CACCCAGCTG
    CGTGTGCCGG GCCAGAGGCT TGCCGAGAAG AGAGGCAGCG CTGACAGTCT AGTGGAGGCG
    GTTTTGATAT CAGAAGGCCT AGGGCTTTAT GCACGGGACC CCAAGTTTGT CGCTTTCGCC
    AAACGTGAGA TCGCAGATGC CTGTCAGATG ACCATTGATG AGATGGAGAG TGCCGCCAGT
    GACTTGCTAA GCCAGAGCAG GAAAGGCAGT TGGACTGGAA ATAATGGACA CTCCTACCAA
    GATGAAACGG CATGGAATGG ACTTTTGTAC AGTGATGAAG ACTCTATACA CTGCCGAGAA
    GAAGAGGAAT TAAGAGATGA GATGGCCTGC GTGTCTTCAT TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031747315.1
    CDS348..6452
    Translation

    Target ORF information:

    RefSeq Version XM_031891455.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calcium channel, voltage-dependent, L type, alpha 1F subunit (cacna1f), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031891455.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGTCAGAAC CAGAGGAGAA TGACAAAGGC CCTGCTCCTC CATACTCCAG CGAGGAGGCT 
    GAGCCGTTCA TGGCAAGTGC AGAGGCAGCG CTGGCCTGTT GGCGATACTC CATGGATGGT
    GGGCCAATGA TGAATGGCAC CGACACCTTA AACTCCACTG CCAGCTCCCA GGCGCAGAAG
    AAACGGCAGT ACCACAGCAA GCACAAGAAC CAGGGAACAG GGGGAGTGCA TAGGAGCCCC
    AGGGCTCTCT TTTGTCTGTA CCTCAGCAAC CCCATTCGAA GGGCCTGCAT CAGCATTGTA
    GAATGGAAAC CATTTGATAT TCTCATTCTC ATGACTATTT TCGCCAACTG CGTGGCGTTG
    GGTGTCTACA TTCCGTTTCC CGAGGACGAT TCCAACATTG CCAATCACAA CCTGGAACAA
    GTGGAGTATA TTTTCCTCAT TATCTTCACC GTGGAGTCCT TCCTAAAGAT AATTGCATAC
    GGGCTGGTCT TGCACCCTAG TGCCTACATC AGAAACGGCT GGAACCTGCT GGACTTTGTC
    ATTGTTGTTA TTGGGTTATT CAGTGTTATT TTGGAACAGT TCTCTCACAA GCAAGGAGAT
    GCCCACCACA TGAGTGGAAA GCCAGGAGGG TTTGATGTGA AAGCTCTGCG TGCATTTCGT
    GTGTTAAGAC CTCTCAGGCT GGTATCTGGA GTCCCAAGCT TGCACATTGT ATTAAATTCC
    ATAATGAAAG CCATGGTTCC CTTATTGCAC ATTGCCTTGC TTGTCCTCTT TGTTATCATC
    ATTTATGCCA TTATTGGGCT GGAGCTCTTC ATTGGTCGGA TGCACAAGAC ATGTTTCTAC
    ATTGGTTCAG ATCTAGTTTC AGAGGAGGAA CCAGCCCCCT GTGCTTTCTC AGGACACGGA
    AGAGAATGTC TCCTCAACAA TACAGAGTGC AGAGGAAAGT GGGAAGGGCC TAACGGGGGA
    ATCACAAACT TTGATAACTT CTTCTTTGCC ATGCTGACTG TGTTCCAATG TATCACTATG
    GAAGGTTGGA CAGATGTTCT CTACTGGATG CAGGATGCCA TGGGTTATGA GCTTCCCTGG
    GTATATTTCG TCAGCCTTGT TATTTTTGGA TCATTCTTTG TTTTGAACCT TGTATTGGGT
    GTGTTGAGTG GAGAATTTTC TAAAGAAAGA GAAAAGGCCA AGGCCCGAGG GGACTTCCAA
    AAGCTGAGGG AGAAACAGCA GATGGAAGAG GATCTCAAGG GATACCTAGA CTGGATCACC
    CAGGCCGAAG ATCTTGAACC TGATCAAGAA ATCAGAGATG AGCGGCACCG CCTGTCTCTG
    GCTGACATCA CTGGCAGGAG GAAGCGGAGA CTTCAATGGC TTTTGCATGC TAACCATTCC
    ACAGAGACCC ACACTAGCCT TCCAGCAAGT GAGACCACTT CTGTGAACAC CGAGAATATA
    GACGAGGAGC ATGCTAACTG CTGTGCCCGC TGTTTGAGCA AGCTGTCACA GACTGGGTGC
    TGCCGTAAGA TAAGGCGGTG GAACCGAGCC TTTCGACGGA AATGCCGCCT GGCTGTTAAA
    TCGGTCACCT TCTACTGGAT GGTTCTGCTC CTCGTCTTCC TCAACACGCT GACCATTGCA
    TCAGAGCATT ACCAACAGCC AGATTGGCTG ACTGAGATTC AAGCGTATGC CAACAAGGTG
    CTTCTGTCTC TATTCACACT GGAGATGCTT CTCAAGATGT ACAGCCTTGG GTTACACGCC
    TACTTTGTGT CCTTCTTTAA CCGCTTTGAT TGCTTCGTGG TTTGTGGTGG GATCTTGGAG
    ACTGTCCTTG TAGAATTTGA CATCATGTCT CCTCTGGGAA TCTCTGTTCT TCGTTGTGTA
    CGACTATTGA GGATCTTTAA GGTCACCAGA CACTGGGCAT CACTAAGTAA TTTGGTGGCT
    TCCCTTTTGA ACTCCATGAA ATCCATTGCT TCTTTGCTGC TGCTTCTCTT CCTCTTCATT
    ATCATCTTCT CCTTGCTCGG AATGCAGCTC TTTGGGGGCA AATTCAACTT TGATGAGACC
    CAGACCAAGA GGAGCACATT TGACACCTTC CCTCAGGCCC TGCTTACTGT CTTCCAGATA
    CTCACAGGTG AGGACTGGAA TGCAGTCATG TACGATGGGA TAATGGCGTA TGGGGGACCA
    TTTTTCCCTG GAATGCTGGT CTGTGTGTAT TTTATCATCC TCTTCATATG TGGTAACTAT
    ATCCTACTCA ATGTCTTTTT GGCCATCGCT GTGGACAATC TGGCTGATGG TGACAACATC
    AATAGCAAAG ATAAAAAGGT GAACAACAAA TCAGAGAAGA AGGATGAAAA CTCTGTCAAG
    GTTCCGTGTG AGGAGGAGAA TGAAAACCAG GAGGAGGAAG GGAGTGAAGC CTCTGAAGAA
    GAGGAAGATG ATGAGGGCAG CCTCCCAGAA TCACACTTGG ATAAAATTGC TGACTTTGCT
    CCAAAGGAGA AGGTTCTTCC CATTCCCGAG GGCAGTGCAT TCTTCATCCT CAGTAACACC
    AACCTCCTCC GCGTTGGGTG CCACAAGCTG ATTCACCACC ACATTTTCAC CAACCTCATT
    TTAGTCTTCA TCATCCTCAG CAGCATTTCC CTGGCAGCCG AAGATCCAAT CCGCGCCCAC
    TCTTTTCGTA ATCATATCCT GGGGTATTTT GATTATGCAT TCACGTCTAT CTTCACAGTG
    GAGATTTTGC TGAAGATGAC AGCATATGGT GCATTCTTGC ACAAGGGCTC CTTCTGCAGG
    AACTGGTTCA ATCTGCTGGA TCTCCTTGTT GTCAGCGTGT CTCTCATCAG TTTTGGAATC
    CATTCTAGCG CTATCTCCGT TGTAAAGATT CTACGTGTTC TGAGAGTACT GCGTCCATTA
    AGAGCCATCA ACAGAGCCAA GGGGCTTAAG CACGTGGTCC AGTGCGTCTT TGTTGCAATC
    CGCACTATCG GTAACATTAT GATAGTGACT ACTTTGCTGC AGTTCATGTT CGCCTGCATA
    GGTGTTCAGC TTTTCAAGGG CAAGTTTTAC AGTTGCACTG ATGAGGCCAA ACGCAGCCCA
    GAGGAGTGCA AGGGCACATT CATTATTTAT AAGGAAGGGG ACGTTTCACA CCCCATGGTG
    CGGGAAAGAA TTTGGCACAA CAGTGACTTC AACTTTGACA ACGTCCTGTC AGGCATGATG
    GCTCTGTTTA CAGTGTCCAC ATTTGAAGGT TGGCCAGCGT TACTATACAA AGCCATTGAT
    GCCTATGGGG AGAACGTAGG ACCTATTTAC AACTACCGTG TGGAGATCTC CATCTTTTTT
    ATTGTCTACA TTATCATCAT TGCCTTCTTC ATGATGAACA TCTTTGTGGG TTTTGTGATT
    ATCACTTTCC GAGCACAAGG AGAGCAGGAG TACAAGAACT GTGAACTGGA CAAGAACCAG
    CGCCAGTGTG TGGAATATGC ATTGAAGGCT CAGCCTCTGC GGCTGTACAT CCCCAAGGCA
    AAGTATCAGT ACAAAGTCTG GTACATGGTC AACTCAACTG GCTTTGAGTA CATCATGTTT
    GTCCTTATCC TGCTCAACAC TGTTGCTTTA GCCATGCAGC ATTATGAGCA GTCCAAGCCC
    TTTAATTATG CAATGGATAT CCTGAATATG GTCTTCACTG GACTCTTCAC CGTGGAAATG
    GTGCTAAAGC TGATGGCATT TCGACCCAAG CATTATTTCT GCGATGCCTG GAACACATTT
    GATGCTCTCA TTGTAGTGGG CAGCCTTGTG GATATTGCAG TCACCGAGGT CAATAATGGA
    TGGCATGTTG GTGAGAGTTC AGAGGATAGC TCTCGTATTT CCATCACATT CTTCCGGCTC
    TTCCGCGTCA TGCGTTTGGT AAAGCTTCTT AGTAAAGGAG AGGGAATCAG GACACTGCTG
    TGGACATTCA TCAAATCCTT CCAGGCTTTG CCATACGTTG CTCTGCTCAT CGCTATGATA
    TTTTTTATCT ACGCTGTCAT CGGCATGCAG ACATTTGGGA AAATTGCAAT GCAAGATGCG
    AGTCAAATCA ACAGAAATAA CAACTTTCAG ACCTTTCCTC AGGCTGTGCT TTTACTGTTC
    AGGTGTGCAA CCGGGGAGGC CTGGCAAGAC ATCATGTTGG CAAGCCTGCC TGGGAAGCGG
    TGTGACAGCG AGTCCGACTT TGGTCCTGGG GAGGAATTTA CCTGTGGGAG TAACTTCGCT
    ATTGTGTATT TCATCAGTTT CTTCATGCTC TGCGCCTTCC TTATCATTAA CCTGTTTGTG
    GCTGTCATTA TGGACAACTT TGACTACCTC ACTAGGGATT GGTCAATTCT GGGCCCACAT
    CATTTAGATG AGTTTAAGAG GATCTGGTCG GAATACGATC CCGAAGCTAA GGGTCGCATC
    AAGCACCTGG ATGTTGTGAC CTTGCTTCGC AGGATCCAGC CTCCACTGGG ATTTGGGAAG
    CTGTGCCCCC ATCGTGTAGC ATGTAAGAGG CTGGTGGCCA TGAATATGCC TTTAAATGCA
    GATGGGACTG TCACCTTTAA TGCTACACTT TTTGCCCTTG TTCGAACATC ACTCAAGATT
    AAAACTGAAG GTAACCTAGA GACTGCCAAT GAGGAACTGA GAGCTGTCAT CAAAAAGATC
    TGGAAAAGAA CCAAGCAGAA GATACTAGAT GAGGTGATTC CCCCTCCTGA AGAGGAGGAG
    GTGACGGTTG GGAAATTTTA TGCCACATTC CTGATCCAGG ATTACTTTCG CAAGTTTCGC
    CGAAGAAAAG AAAAGGGGCT CCTGGGAACA GAGAATTTGC CTTGTAATTC CACAACACTG
    CAGGCAGGGC TGAGGAGCCT ACAGGATCTT GGTTCAGAGA TACGACGAGT GATATCCACT
    GAGCTAGAGG AGGAAGAAGA TATCTCTGGA TTCAGAGCAC TGGGGCTTCA GGTTTCAGAA
    GACAAATCAT TGTGCGAAAA CTTAACTTTG GTGCAGGCAA CTGAAAATAA GATTTCTGGA
    TCTGTAGTAT CTCTTACTAA CTCCATTGGA GGAGGAAGAG AAGAGCAGCT ACCAATGTCT
    CGAAGATCAT CACTAATAAA TCGGTCTGTA GGAGGATCTA AGGACAGTTT GTTGGTCCCA
    CCCACACCAC CACCCAATGT GTCGTCCAGC ATGAAGAGGA GGCAGATGAC CACACGGAGG
    AGAAGCTCAG AAGGCAGCGG TATCACTCCC ACTGTGCATG AAGAGGAAGA GGAGAGTGCA
    GCAGAACATG AGCTGAGATC TAGACCTCCC TCACCGGGGT ACGACCAAAG CAATGAGATA
    CAGCGCCAGT CTAGTTTGGA GGAGTCTCAG CAGCGCTCAG CATCTCCTCA GGAAAGAGTG
    AAAGTTCTGC CTCCTCCGGC TGCCCCTCCA TCTAGTTTCA GAGCGAATGG CACCTTGGAA
    GGAAGAGCAA CCCAAAGAAG GCGCCTGCTG CCTCCTACCC CAGGTACCAA GTCTGCATTT
    AGCATCCAGT GCCTGAAGAG ACAGGGGAGC TGTGATGATA TACCCATCCC AGGAACATAC
    CATCAGAATG CACCCCCATG CAGAACAAGA GGCCAGGTTT ATGGAAGTAC CGATTCTTGG
    CAAAGAAATA GTTACTCCAC CAATGGCACT CACTCATGGG CCAACCCACC AAAGCGTGGG
    CGCCTTCTTT ATGCACCCCT TATATTGGTG GAGGAAGACA GCGCCCTGCC TACTATTCCA
    TGCACTCGCT GGTATCAGGA AGAGGATAGC AAAGCACCCT ATTGCCACTA CACCCAGCTG
    CGTGTGCCGG GCCAGAGGCT TGCCGAGAAG AGAGGCAGCG CTGACAGTCT AGTGGAGGCG
    GTTTTGATAT CAGAAGGCCT AGGGCTTTAT GCACGGGACC CCAAGTTTGT CGCTTTCGCC
    AAACGTGAGA TCGCAGATGC CTGTCAGATG ACCATTGATG AGATGGAGAG TGCCGCCAGT
    GACTTGCTAA GCCAGAGCAG GAAAGGCAGT TGGACTGGAA ATAATGGACA CTCCTACCAA
    GATGAAACGG CATGGAATGG ACTTTTGTAC AGTGATGAAG ACTCTATACA CTGCCGAGAA
    GAAGAGGAAT TAAGAGATGA GATGGCCTGC GTGTCTTCAT TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa64766
    Clone ID Related Accession (Same CDS sequence) XM_031891456.1
    Accession Version XM_031891456.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6021bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product voltage-dependent L-type calcium channel subunit alpha-1F isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGTCAGAAC CAGAGGAGAA TGACAAAGGC CCTGCTCCTC CATACTCCAG CGAGGAGGCT 
    GAGCCGTTCA TGGCAAGTGC AGAGGCAGCG CTGGCCTGTT GGCGATACTC CATGGATGGT
    GGGCCAATGA TGAATGGCAC CGACACCTTA AACTCCACTG CCAGCTCCCA GGCGCAGAAG
    AAACGGCAGT ACCACAGCAA GCACAAGAAC CAGGGAACAG GGGGAGTGCA TAGGAGCCCC
    AGGGCTCTCT TTTGTCTGTA CCTCAGCAAC CCCATTCGAA GGGCCTGCAT CAGCATTGTA
    GAATGGAAAC CATTTGATAT TCTCATTCTC ATGACTATTT TCGCCAACTG CGTGGCGTTG
    GGTGTCTACA TTCCGTTTCC CGAGGACGAT TCCAACATTG CCAATCACAA CCTGGAACAA
    GTGGAGTATA TTTTCCTCAT TATCTTCACC GTGGAGTCCT TCCTAAAGAT AATTGCATAC
    GGGCTGGTCT TGCACCCTAG TGCCTACATC AGAAACGGCT GGAACCTGCT GGACTTTGTC
    ATTGTTGTTA TTGGGTTATT CAGTGTTATT TTGGAACAGT TCTCTCACAA GCAAGGAGAT
    GCCCACCACA TGAGTGGAAA GCCAGGAGGG TTTGATGTGA AAGCTCTGCG TGCATTTCGT
    GTGTTAAGAC CTCTCAGGCT GGTATCTGGA GTCCCAAGCT TGCACATTGT ATTAAATTCC
    ATAATGAAAG CCATGGTTCC CTTATTGCAC ATTGCCTTGC TTGTCCTCTT TGTTATCATC
    ATTTATGCCA TTATTGGGCT GGAGCTCTTC ATTGGTCGGA TGCACAAGAC ATGTTTCTAC
    ATTGGTTCAG ATCTAGTTTC AGAGGAGGAA CCAGCCCCCT GTGCTTTCTC AGGACACGGA
    AGAGAATGTC TCCTCAACAA TACAGAGTGC AGAGGAAAGT GGGAAGGGCC TAACGGGGGA
    ATCACAAACT TTGATAACTT CTTCTTTGCC ATGCTGACTG TGTTCCAATG TATCACTATG
    GAAGGTTGGA CAGATGTTCT CTACTGGATG CAGGATGCCA TGGGTTATGA GCTTCCCTGG
    GTATATTTCG TCAGCCTTGT TATTTTTGGA TCATTCTTTG TTTTGAACCT TGTATTGGGT
    GTGTTGAGTG GAGAATTTTC TAAAGAAAGA GAAAAGGCCA AGGCCCGAGG GGACTTCCAA
    AAGCTGAGGG AGAAACAGCA GATGGAAGAG GATCTCAAGG GATACCTAGA CTGGATCACC
    CAGGCCGAAG ATCTTGAACC TGATCAAGAA ATCAGAGATG AGCGGCACCC TAGCCTTCCA
    GCAAGTGAGA CCACTTCTGT GAACACCGAG AATATAGACG AGGAGCATGC TAACTGCTGT
    GCCCGCTGTT TGAGCAAGCT GTCACAGACT GGGTGCTGCC GTAAGATAAG GCGGTGGAAC
    CGAGCCTTTC GACGGAAATG CCGCCTGGCT GTTAAATCGG TCACCTTCTA CTGGATGGTT
    CTGCTCCTCG TCTTCCTCAA CACGCTGACC ATTGCATCAG AGCATTACCA ACAGCCAGAT
    TGGCTGACTG AGATTCAAGC GTATGCCAAC AAGGTGCTTC TGTCTCTATT CACACTGGAG
    ATGCTTCTCA AGATGTACAG CCTTGGGTTA CACGCCTACT TTGTGTCCTT CTTTAACCGC
    TTTGATTGCT TCGTGGTTTG TGGTGGGATC TTGGAGACTG TCCTTGTAGA ATTTGACATC
    ATGTCTCCTC TGGGAATCTC TGTTCTTCGT TGTGTACGAC TATTGAGGAT CTTTAAGGTC
    ACCAGACACT GGGCATCACT AAGTAATTTG GTGGCTTCCC TTTTGAACTC CATGAAATCC
    ATTGCTTCTT TGCTGCTGCT TCTCTTCCTC TTCATTATCA TCTTCTCCTT GCTCGGAATG
    CAGCTCTTTG GGGGCAAATT CAACTTTGAT GAGACCCAGA CCAAGAGGAG CACATTTGAC
    ACCTTCCCTC AGGCCCTGCT TACTGTCTTC CAGATACTCA CAGGTGAGGA CTGGAATGCA
    GTCATGTACG ATGGGATAAT GGCGTATGGG GGACCATTTT TCCCTGGAAT GCTGGTCTGT
    GTGTATTTTA TCATCCTCTT CATATGTGGT AACTATATCC TACTCAATGT CTTTTTGGCC
    ATCGCTGTGG ACAATCTGGC TGATGGTGAC AACATCAATA GCAAAGATAA AAAGGTGAAC
    AACAAATCAG AGAAGAAGGA TGAAAACTCT GTCAAGGTTC CGTGTGAGGA GGAGAATGAA
    AACCAGGAGG AGGAAGGGAG TGAAGCCTCT GAAGAAGAGG AAGATGATGA GGGCAGCCTC
    CCAGAATCAC ACTTGGATAA AATTGCTGAC TTTGCTCCAA AGGAGAAGGT TCTTCCCATT
    CCCGAGGGCA GTGCATTCTT CATCCTCAGT AACACCAACC TCCTCCGCGT TGGGTGCCAC
    AAGCTGATTC ACCACCACAT TTTCACCAAC CTCATTTTAG TCTTCATCAT CCTCAGCAGC
    ATTTCCCTGG CAGCCGAAGA TCCAATCCGC GCCCACTCTT TTCGTAATCA TATCCTGGGG
    TATTTTGATT ATGCATTCAC GTCTATCTTC ACAGTGGAGA TTTTGCTGAA GATGACAGCA
    TATGGTGCAT TCTTGCACAA GGGCTCCTTC TGCAGGAACT GGTTCAATCT GCTGGATCTC
    CTTGTTGTCA GCGTGTCTCT CATCAGTTTT GGAATCCATT CTAGCGCTAT CTCCGTTGTA
    AAGATTCTAC GTGTTCTGAG AGTACTGCGT CCATTAAGAG CCATCAACAG AGCCAAGGGG
    CTTAAGCACG TGGTCCAGTG CGTCTTTGTT GCAATCCGCA CTATCGGTAA CATTATGATA
    GTGACTACTT TGCTGCAGTT CATGTTCGCC TGCATAGGTG TTCAGCTTTT CAAGGGCAAG
    TTTTACAGTT GCACTGATGA GGCCAAACGC AGCCCAGAGG AGTGCAAGGG CACATTCATT
    ATTTATAAGG AAGGGGACGT TTCACACCCC ATGGTGCGGG AAAGAATTTG GCACAACAGT
    GACTTCAACT TTGACAACGT CCTGTCAGGC ATGATGGCTC TGTTTACAGT GTCCACATTT
    GAAGGTTGGC CAGCGTTACT ATACAAAGCC ATTGATGCCT ATGGGGAGAA CGTAGGACCT
    ATTTACAACT ACCGTGTGGA GATCTCCATC TTTTTTATTG TCTACATTAT CATCATTGCC
    TTCTTCATGA TGAACATCTT TGTGGGTTTT GTGATTATCA CTTTCCGAGC ACAAGGAGAG
    CAGGAGTACA AGAACTGTGA ACTGGACAAG AACCAGCGCC AGTGTGTGGA ATATGCATTG
    AAGGCTCAGC CTCTGCGGCT GTACATCCCC AAGGCAAAGT ATCAGTACAA AGTCTGGTAC
    ATGGTCAACT CAACTGGCTT TGAGTACATC ATGTTTGTCC TTATCCTGCT CAACACTGTT
    GCTTTAGCCA TGCAGCATTA TGAGCAGTCC AAGCCCTTTA ATTATGCAAT GGATATCCTG
    AATATGGTCT TCACTGGACT CTTCACCGTG GAAATGGTGC TAAAGCTGAT GGCATTTCGA
    CCCAAGCATT ATTTCTGCGA TGCCTGGAAC ACATTTGATG CTCTCATTGT AGTGGGCAGC
    CTTGTGGATA TTGCAGTCAC CGAGGTCAAT AATGGATGGC ATGTTGGTGA GAGTTCAGAG
    GATAGCTCTC GTATTTCCAT CACATTCTTC CGGCTCTTCC GCGTCATGCG TTTGGTAAAG
    CTTCTTAGTA AAGGAGAGGG AATCAGGACA CTGCTGTGGA CATTCATCAA ATCCTTCCAG
    GCTTTGCCAT ACGTTGCTCT GCTCATCGCT ATGATATTTT TTATCTACGC TGTCATCGGC
    ATGCAGACAT TTGGGAAAAT TGCAATGCAA GATGCGAGTC AAATCAACAG AAATAACAAC
    TTTCAGACCT TTCCTCAGGC TGTGCTTTTA CTGTTCAGGT GTGCAACCGG GGAGGCCTGG
    CAAGACATCA TGTTGGCAAG CCTGCCTGGG AAGCGGTGTG ACAGCGAGTC CGACTTTGGT
    CCTGGGGAGG AATTTACCTG TGGGAGTAAC TTCGCTATTG TGTATTTCAT CAGTTTCTTC
    ATGCTCTGCG CCTTCCTTAT CATTAACCTG TTTGTGGCTG TCATTATGGA CAACTTTGAC
    TACCTCACTA GGGATTGGTC AATTCTGGGC CCACATCATT TAGATGAGTT TAAGAGGATC
    TGGTCGGAAT ACGATCCCGA AGCTAAGGGT CGCATCAAGC ACCTGGATGT TGTGACCTTG
    CTTCGCAGGA TCCAGCCTCC ACTGGGATTT GGGAAGCTGT GCCCCCATCG TGTAGCATGT
    AAGAGGCTGG TGGCCATGAA TATGCCTTTA AATGCAGATG GGACTGTCAC CTTTAATGCT
    ACACTTTTTG CCCTTGTTCG AACATCACTC AAGATTAAAA CTGAAGGTAA CCTAGAGACT
    GCCAATGAGG AACTGAGAGC TGTCATCAAA AAGATCTGGA AAAGAACCAA GCAGAAGATA
    CTAGATGAGG TGATTCCCCC TCCTGAAGAG GAGGAGGTGA CGGTTGGGAA ATTTTATGCC
    ACATTCCTGA TCCAGGATTA CTTTCGCAAG TTTCGCCGAA GAAAAGAAAA GGGGCTCCTG
    GGAACAGAGA ATTTGCCTTG TAATTCCACA ACACTGCAGG CAGGGCTGAG GAGCCTACAG
    GATCTTGGTT CAGAGATACG ACGAGTGATA TCCACTGAGC TAGAGGAGGA AGAAGATATC
    TCTGGATTCA GAGCACTGGG GCTTCAGGTT TCAGAAGACA AATCATTGTG CGAAAACTTA
    ACTTTGGTGC AGGCAACTGA AAATAAGATT TCTGGATCTG TAGTATCTCT TACTAACTCC
    ATTGGAGGAG GAAGAGAAGA GCAGCTACCA ATGTCTCGAA GATCATCACT AATAAATCGG
    TCTGTAGGAG GATCTAAGGA CAGTTTGTTG GTCCCACCCA CACCACCACC CAATGTGTCG
    TCCAGCATGA AGAGGAGGCA GATGACCACA CGGAGGAGAA GCTCAGAAGG CAGCGGTATC
    ACTCCCACTG TGCATGAAGA GGAAGAGGAG AGTGCAGCAG AACATGAGCT GAGATCTAGA
    CCTCCCTCAC CGGGGTACGA CCAAAGCAAT GAGATACAGC GCCAGTCTAG TTTGGAGGAG
    TCTCAGCAGC GCTCAGCATC TCCTCAGGAA AGAGTGAAAG TTCTGCCTCC TCCGGCTGCC
    CCTCCATCTA GTTTCAGAGC GAATGGCACC TTGGAAGGAA GAGCAACCCA AAGAAGGCGC
    CTGCTGCCTC CTACCCCAGG TACCAAGTCT GCATTTAGCA TCCAGTGCCT GAAGAGACAG
    GGGAGCTGTG ATGATATACC CATCCCAGGA ACATACCATC AGAATGCACC CCCATGCAGA
    ACAAGAGGCC AGGTTTATGG AAGTACCGAT TCTTGGCAAA GAAATAGTTA CTCCACCAAT
    GGCACTCACT CATGGGCCAA CCCACCAAAG CGTGGGCGCC TTCTTTATGC ACCCCTTATA
    TTGGTGGAGG AAGACAGCGC CCTGCCTACT ATTCCATGCA CTCGCTGGTA TCAGGAAGAG
    GATAGCAAAG CACCCTATTG CCACTACACC CAGCTGCGTG TGCCGGGCCA GAGGCTTGCC
    GAGAAGAGAG GCAGCGCTGA CAGTCTAGTG GAGGCGGTTT TGATATCAGA AGGCCTAGGG
    CTTTATGCAC GGGACCCCAA GTTTGTCGCT TTCGCCAAAC GTGAGATCGC AGATGCCTGT
    CAGATGACCA TTGATGAGAT GGAGAGTGCC GCCAGTGACT TGCTAAGCCA GAGCAGGAAA
    GGCAGTTGGA CTGGAAATAA TGGACACTCC TACCAAGATG AAACGGCATG GAATGGACTT
    TTGTACAGTG ATGAAGACTC TATACACTGC CGAGAAGAAG AGGAATTAAG AGATGAGATG
    GCCTGCGTGT CTTCATTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031747316.1
    CDS348..6368
    Translation

    Target ORF information:

    RefSeq Version XM_031891456.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calcium channel, voltage-dependent, L type, alpha 1F subunit (cacna1f), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031891456.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGTCAGAAC CAGAGGAGAA TGACAAAGGC CCTGCTCCTC CATACTCCAG CGAGGAGGCT 
    GAGCCGTTCA TGGCAAGTGC AGAGGCAGCG CTGGCCTGTT GGCGATACTC CATGGATGGT
    GGGCCAATGA TGAATGGCAC CGACACCTTA AACTCCACTG CCAGCTCCCA GGCGCAGAAG
    AAACGGCAGT ACCACAGCAA GCACAAGAAC CAGGGAACAG GGGGAGTGCA TAGGAGCCCC
    AGGGCTCTCT TTTGTCTGTA CCTCAGCAAC CCCATTCGAA GGGCCTGCAT CAGCATTGTA
    GAATGGAAAC CATTTGATAT TCTCATTCTC ATGACTATTT TCGCCAACTG CGTGGCGTTG
    GGTGTCTACA TTCCGTTTCC CGAGGACGAT TCCAACATTG CCAATCACAA CCTGGAACAA
    GTGGAGTATA TTTTCCTCAT TATCTTCACC GTGGAGTCCT TCCTAAAGAT AATTGCATAC
    GGGCTGGTCT TGCACCCTAG TGCCTACATC AGAAACGGCT GGAACCTGCT GGACTTTGTC
    ATTGTTGTTA TTGGGTTATT CAGTGTTATT TTGGAACAGT TCTCTCACAA GCAAGGAGAT
    GCCCACCACA TGAGTGGAAA GCCAGGAGGG TTTGATGTGA AAGCTCTGCG TGCATTTCGT
    GTGTTAAGAC CTCTCAGGCT GGTATCTGGA GTCCCAAGCT TGCACATTGT ATTAAATTCC
    ATAATGAAAG CCATGGTTCC CTTATTGCAC ATTGCCTTGC TTGTCCTCTT TGTTATCATC
    ATTTATGCCA TTATTGGGCT GGAGCTCTTC ATTGGTCGGA TGCACAAGAC ATGTTTCTAC
    ATTGGTTCAG ATCTAGTTTC AGAGGAGGAA CCAGCCCCCT GTGCTTTCTC AGGACACGGA
    AGAGAATGTC TCCTCAACAA TACAGAGTGC AGAGGAAAGT GGGAAGGGCC TAACGGGGGA
    ATCACAAACT TTGATAACTT CTTCTTTGCC ATGCTGACTG TGTTCCAATG TATCACTATG
    GAAGGTTGGA CAGATGTTCT CTACTGGATG CAGGATGCCA TGGGTTATGA GCTTCCCTGG
    GTATATTTCG TCAGCCTTGT TATTTTTGGA TCATTCTTTG TTTTGAACCT TGTATTGGGT
    GTGTTGAGTG GAGAATTTTC TAAAGAAAGA GAAAAGGCCA AGGCCCGAGG GGACTTCCAA
    AAGCTGAGGG AGAAACAGCA GATGGAAGAG GATCTCAAGG GATACCTAGA CTGGATCACC
    CAGGCCGAAG ATCTTGAACC TGATCAAGAA ATCAGAGATG AGCGGCACCC TAGCCTTCCA
    GCAAGTGAGA CCACTTCTGT GAACACCGAG AATATAGACG AGGAGCATGC TAACTGCTGT
    GCCCGCTGTT TGAGCAAGCT GTCACAGACT GGGTGCTGCC GTAAGATAAG GCGGTGGAAC
    CGAGCCTTTC GACGGAAATG CCGCCTGGCT GTTAAATCGG TCACCTTCTA CTGGATGGTT
    CTGCTCCTCG TCTTCCTCAA CACGCTGACC ATTGCATCAG AGCATTACCA ACAGCCAGAT
    TGGCTGACTG AGATTCAAGC GTATGCCAAC AAGGTGCTTC TGTCTCTATT CACACTGGAG
    ATGCTTCTCA AGATGTACAG CCTTGGGTTA CACGCCTACT TTGTGTCCTT CTTTAACCGC
    TTTGATTGCT TCGTGGTTTG TGGTGGGATC TTGGAGACTG TCCTTGTAGA ATTTGACATC
    ATGTCTCCTC TGGGAATCTC TGTTCTTCGT TGTGTACGAC TATTGAGGAT CTTTAAGGTC
    ACCAGACACT GGGCATCACT AAGTAATTTG GTGGCTTCCC TTTTGAACTC CATGAAATCC
    ATTGCTTCTT TGCTGCTGCT TCTCTTCCTC TTCATTATCA TCTTCTCCTT GCTCGGAATG
    CAGCTCTTTG GGGGCAAATT CAACTTTGAT GAGACCCAGA CCAAGAGGAG CACATTTGAC
    ACCTTCCCTC AGGCCCTGCT TACTGTCTTC CAGATACTCA CAGGTGAGGA CTGGAATGCA
    GTCATGTACG ATGGGATAAT GGCGTATGGG GGACCATTTT TCCCTGGAAT GCTGGTCTGT
    GTGTATTTTA TCATCCTCTT CATATGTGGT AACTATATCC TACTCAATGT CTTTTTGGCC
    ATCGCTGTGG ACAATCTGGC TGATGGTGAC AACATCAATA GCAAAGATAA AAAGGTGAAC
    AACAAATCAG AGAAGAAGGA TGAAAACTCT GTCAAGGTTC CGTGTGAGGA GGAGAATGAA
    AACCAGGAGG AGGAAGGGAG TGAAGCCTCT GAAGAAGAGG AAGATGATGA GGGCAGCCTC
    CCAGAATCAC ACTTGGATAA AATTGCTGAC TTTGCTCCAA AGGAGAAGGT TCTTCCCATT
    CCCGAGGGCA GTGCATTCTT CATCCTCAGT AACACCAACC TCCTCCGCGT TGGGTGCCAC
    AAGCTGATTC ACCACCACAT TTTCACCAAC CTCATTTTAG TCTTCATCAT CCTCAGCAGC
    ATTTCCCTGG CAGCCGAAGA TCCAATCCGC GCCCACTCTT TTCGTAATCA TATCCTGGGG
    TATTTTGATT ATGCATTCAC GTCTATCTTC ACAGTGGAGA TTTTGCTGAA GATGACAGCA
    TATGGTGCAT TCTTGCACAA GGGCTCCTTC TGCAGGAACT GGTTCAATCT GCTGGATCTC
    CTTGTTGTCA GCGTGTCTCT CATCAGTTTT GGAATCCATT CTAGCGCTAT CTCCGTTGTA
    AAGATTCTAC GTGTTCTGAG AGTACTGCGT CCATTAAGAG CCATCAACAG AGCCAAGGGG
    CTTAAGCACG TGGTCCAGTG CGTCTTTGTT GCAATCCGCA CTATCGGTAA CATTATGATA
    GTGACTACTT TGCTGCAGTT CATGTTCGCC TGCATAGGTG TTCAGCTTTT CAAGGGCAAG
    TTTTACAGTT GCACTGATGA GGCCAAACGC AGCCCAGAGG AGTGCAAGGG CACATTCATT
    ATTTATAAGG AAGGGGACGT TTCACACCCC ATGGTGCGGG AAAGAATTTG GCACAACAGT
    GACTTCAACT TTGACAACGT CCTGTCAGGC ATGATGGCTC TGTTTACAGT GTCCACATTT
    GAAGGTTGGC CAGCGTTACT ATACAAAGCC ATTGATGCCT ATGGGGAGAA CGTAGGACCT
    ATTTACAACT ACCGTGTGGA GATCTCCATC TTTTTTATTG TCTACATTAT CATCATTGCC
    TTCTTCATGA TGAACATCTT TGTGGGTTTT GTGATTATCA CTTTCCGAGC ACAAGGAGAG
    CAGGAGTACA AGAACTGTGA ACTGGACAAG AACCAGCGCC AGTGTGTGGA ATATGCATTG
    AAGGCTCAGC CTCTGCGGCT GTACATCCCC AAGGCAAAGT ATCAGTACAA AGTCTGGTAC
    ATGGTCAACT CAACTGGCTT TGAGTACATC ATGTTTGTCC TTATCCTGCT CAACACTGTT
    GCTTTAGCCA TGCAGCATTA TGAGCAGTCC AAGCCCTTTA ATTATGCAAT GGATATCCTG
    AATATGGTCT TCACTGGACT CTTCACCGTG GAAATGGTGC TAAAGCTGAT GGCATTTCGA
    CCCAAGCATT ATTTCTGCGA TGCCTGGAAC ACATTTGATG CTCTCATTGT AGTGGGCAGC
    CTTGTGGATA TTGCAGTCAC CGAGGTCAAT AATGGATGGC ATGTTGGTGA GAGTTCAGAG
    GATAGCTCTC GTATTTCCAT CACATTCTTC CGGCTCTTCC GCGTCATGCG TTTGGTAAAG
    CTTCTTAGTA AAGGAGAGGG AATCAGGACA CTGCTGTGGA CATTCATCAA ATCCTTCCAG
    GCTTTGCCAT ACGTTGCTCT GCTCATCGCT ATGATATTTT TTATCTACGC TGTCATCGGC
    ATGCAGACAT TTGGGAAAAT TGCAATGCAA GATGCGAGTC AAATCAACAG AAATAACAAC
    TTTCAGACCT TTCCTCAGGC TGTGCTTTTA CTGTTCAGGT GTGCAACCGG GGAGGCCTGG
    CAAGACATCA TGTTGGCAAG CCTGCCTGGG AAGCGGTGTG ACAGCGAGTC CGACTTTGGT
    CCTGGGGAGG AATTTACCTG TGGGAGTAAC TTCGCTATTG TGTATTTCAT CAGTTTCTTC
    ATGCTCTGCG CCTTCCTTAT CATTAACCTG TTTGTGGCTG TCATTATGGA CAACTTTGAC
    TACCTCACTA GGGATTGGTC AATTCTGGGC CCACATCATT TAGATGAGTT TAAGAGGATC
    TGGTCGGAAT ACGATCCCGA AGCTAAGGGT CGCATCAAGC ACCTGGATGT TGTGACCTTG
    CTTCGCAGGA TCCAGCCTCC ACTGGGATTT GGGAAGCTGT GCCCCCATCG TGTAGCATGT
    AAGAGGCTGG TGGCCATGAA TATGCCTTTA AATGCAGATG GGACTGTCAC CTTTAATGCT
    ACACTTTTTG CCCTTGTTCG AACATCACTC AAGATTAAAA CTGAAGGTAA CCTAGAGACT
    GCCAATGAGG AACTGAGAGC TGTCATCAAA AAGATCTGGA AAAGAACCAA GCAGAAGATA
    CTAGATGAGG TGATTCCCCC TCCTGAAGAG GAGGAGGTGA CGGTTGGGAA ATTTTATGCC
    ACATTCCTGA TCCAGGATTA CTTTCGCAAG TTTCGCCGAA GAAAAGAAAA GGGGCTCCTG
    GGAACAGAGA ATTTGCCTTG TAATTCCACA ACACTGCAGG CAGGGCTGAG GAGCCTACAG
    GATCTTGGTT CAGAGATACG ACGAGTGATA TCCACTGAGC TAGAGGAGGA AGAAGATATC
    TCTGGATTCA GAGCACTGGG GCTTCAGGTT TCAGAAGACA AATCATTGTG CGAAAACTTA
    ACTTTGGTGC AGGCAACTGA AAATAAGATT TCTGGATCTG TAGTATCTCT TACTAACTCC
    ATTGGAGGAG GAAGAGAAGA GCAGCTACCA ATGTCTCGAA GATCATCACT AATAAATCGG
    TCTGTAGGAG GATCTAAGGA CAGTTTGTTG GTCCCACCCA CACCACCACC CAATGTGTCG
    TCCAGCATGA AGAGGAGGCA GATGACCACA CGGAGGAGAA GCTCAGAAGG CAGCGGTATC
    ACTCCCACTG TGCATGAAGA GGAAGAGGAG AGTGCAGCAG AACATGAGCT GAGATCTAGA
    CCTCCCTCAC CGGGGTACGA CCAAAGCAAT GAGATACAGC GCCAGTCTAG TTTGGAGGAG
    TCTCAGCAGC GCTCAGCATC TCCTCAGGAA AGAGTGAAAG TTCTGCCTCC TCCGGCTGCC
    CCTCCATCTA GTTTCAGAGC GAATGGCACC TTGGAAGGAA GAGCAACCCA AAGAAGGCGC
    CTGCTGCCTC CTACCCCAGG TACCAAGTCT GCATTTAGCA TCCAGTGCCT GAAGAGACAG
    GGGAGCTGTG ATGATATACC CATCCCAGGA ACATACCATC AGAATGCACC CCCATGCAGA
    ACAAGAGGCC AGGTTTATGG AAGTACCGAT TCTTGGCAAA GAAATAGTTA CTCCACCAAT
    GGCACTCACT CATGGGCCAA CCCACCAAAG CGTGGGCGCC TTCTTTATGC ACCCCTTATA
    TTGGTGGAGG AAGACAGCGC CCTGCCTACT ATTCCATGCA CTCGCTGGTA TCAGGAAGAG
    GATAGCAAAG CACCCTATTG CCACTACACC CAGCTGCGTG TGCCGGGCCA GAGGCTTGCC
    GAGAAGAGAG GCAGCGCTGA CAGTCTAGTG GAGGCGGTTT TGATATCAGA AGGCCTAGGG
    CTTTATGCAC GGGACCCCAA GTTTGTCGCT TTCGCCAAAC GTGAGATCGC AGATGCCTGT
    CAGATGACCA TTGATGAGAT GGAGAGTGCC GCCAGTGACT TGCTAAGCCA GAGCAGGAAA
    GGCAGTTGGA CTGGAAATAA TGGACACTCC TACCAAGATG AAACGGCATG GAATGGACTT
    TTGTACAGTG ATGAAGACTC TATACACTGC CGAGAAGAAG AGGAATTAAG AGATGAGATG
    GCCTGCGTGT CTTCATTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.