vps54 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	vps54
Entrez Gene ID	100380095
Full Name	VPS54 GARP complex subunit
General protein information	Preferred Names VPS54 GARP complex subunit Names vacuolar protein sorting-associated protein 54 vacuolar protein sorting 54 homolog
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018094167.1	XP_017949656.1	vacuolar protein sorting-associated protein 54 isoform X2
XM_004914708.4	XP_004914765.1	vacuolar protein sorting-associated protein 54
XM_002939319.4	XP_002939365.2	vacuolar protein sorting-associated protein 54 isoform X1
XM_004914708.3	XP_004914765.1	vacuolar protein sorting-associated protein 54 isoform X1
XM_002939319.5	XP_002939365.2	vacuolar protein sorting-associated protein 54

Pathways from BioSystems

Homologies

Homo sapiens (human)	VPS54	NP_057600.2
Canis lupus familiaris (dog)	VPS54	XP_865972.2
	VPS54	XP_001087667.1
Bos taurus (cattle)	VPS54	NP_001098930.1
Mus musculus (house mouse)	Vps54	NP_620692.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008353	XP_317102.3
Pan troglodytes (chimpanzee)	VPS54	XP_515511.2
Rattus norvegicus (Norway rat)	Vps54	NP_775170.1
Gallus gallus (chicken)	VPS54	XP_419349.3
Oryza sativa (rice)	Os04g0212100	NP_001173796.1
Caenorhabditis elegans (roundworm)	vps-54	NP_001256226.1
Xenopus tropicalis (tropical clawed frog)	vps54	XP_004914765.1
Danio rerio (zebrafish)	vps54	NP_001124248.1
Drosophila melanogaster (fruit fly)	scat	NP_524905.1
Arabidopsis thaliana (thale cress)	VPS54	NP_849409.2

Gene Ontology

The following vps54 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the vps54 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa10788	XM_002939319.5 Latest version!	Xenopus tropicalis VPS54 GARP complex subunit (vps54), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa10788	XM_004914708.4 Latest version!	Xenopus tropicalis VPS54 GARP complex subunit (vps54), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa44599	XM_018094167.1 Latest version!	Xenopus tropicalis VPS54 GARP complex subunit (vps54), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
OXa10788	XM_004914708.3	Xenopus tropicalis VPS54 GARP complex subunit (vps54), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa10788	XM_002939319.4	Xenopus tropicalis VPS54 GARP complex subunit (vps54), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

vps54 ( XM_002939319.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002939365.2 Xenopus tropicalis VPS54 GARP complex subunit (vps54), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa10788
Clone ID Related Accession (Same CDS sequence)	XM_004914708.4 , XM_002939319.4 , XM_004914708.3 , XM_002939319.5
Accession Version	XM_002939319.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2946bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	vacuolar protein sorting-associated protein 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002939319.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGCAA TGGCCTCCAG CCACAGCTCT TCACTAGTGC CTCACTCGAA CAGCAACAAA 
GCTTTTTACA AAAAGGGTCT TCAGCCTGCT CAGCAAGTGG GAATGGTGCG ATCACTGCCT 
GATGTCTGCC CCAAGGAGCC TACAGGTGAT ACTAAGAGTT TGCGTGATGA ACCATCTGTA 
GTTGCAGATC AGCACAAATG GACAATTTAT AATTCTAAAG TAAACCTTCC GGCAGCATTA 
AATGACCCCC GCCTTGCAAA ACGGGAATCA GACTTTTTCA CAAAAACATG GGGGCAAGAC 
TTTGTGGAAA GTGAAGTCCT ACCATCACCC TACCTCCCAG CAATTACTAA GGAACAGTTT 
CTATCATATT CGCAGCAAAC CATCAGTAGA GAAAAAAATC ATGAAAAATG TAAGAGTGTT 
TGCCCACTAA AGGTGGACTT TGACAGACCA GCCTATTCTC ATGTTCTCAA ATCGAGAGCA 
GATCTGGAAC AAGTACCTAA GATCTTTATG AAGCCAGACT TTGCCTTGGA GGACTCCTTA 
ACATTTAATG CTGTTTTGCC ATGGTCTCAT TTTAGCAGTG CCGGAGGGAA AGGAAGCAGA 
GATGCAGCAT CCTCGAAATT ACTTCAAGAA AAGCTAAGCC ACTATTTGGA TATTGTTGAG 
GTTAACATCG CTCACCAAAT CTCCTTGCGC TCTGAAGCAT TTTTCCATGC TATGACCTCT 
CAACATGAGC TGCAGGACTA CCTCAGGAAG ACTTCCCAGG CTGTTAAAAT GCTGCGAGAC 
AAGATCTCTC AGATAGACAG AGTCATGTGT GAGGGATCTC TGCGGATCTT ACGGCAAGCA 
CTGACCAGGA ACAACTGCAT TAAAATATAC AATAAGCTCA AGTTAATGGC AACAGTGCAT 
CAAACTCAGC CTACTGTACA GCTTCTATTA TCAACCTCTG AGTATGTAGG AGCTTTGGAC 
TTAATAGCAA CGACACAAGA GGTCTTGCAG CAGGAGTTAC AGGGTATTCA CAGTTTTCGA 
CATCTCAGCT CACAACTTTG CGAAATGGAA AAACTCATTG ATAAAATGAT CATCTCTGAA 
TTTACTTCGT ATGCTCGCAG CGATTTAAAC AGACCTTTAG AAGAAGACAG TCATGTAGTG 
GAAGAGGAAA GACTGGTCTC TCTCGTATTT GGGATGTTAA AGCAGCGAAA ACTGGATTTC 
TTTGAGATTT ACTCAGACGA AATGATGGCA ACAGCAAAAA ATATCATCAA GCAGTGTGTG 
GTTAACACTG TGTCTCAGAT TGAGGAAATA GACTCAGAAG TTGTTACAAA GCTTACTGAT 
CAGATTCGGA TGATGAATTT TTCTCAGTGG TTTGGCTTGC TTCAGAATAT TTTTTCTAAG 
TTTACAATTT TTATTGAGAG AGTTAAGGCA ACGCTAAGCG TCATCCGTAA TGTAGTGCTC 
ATGGTTTTAG ACAAAAATCA GCGGGTGAGG GAAATGGAAA TGTTGGCAGC TCAACAGAAT 
CTCAGAAGAG AAAGCAATGT GGACAATGAA TTGGCGTACT TGACTCATGA AGGCTTATTT 
ATAAGTGATG CTTTCGGTGA AGGTGAGCTT CCCAATGCTG CTACCAGCAC TAACTTTCAA 
AGGAACACAT CACCCAGCAG TAGCGAGCCC TCTGGCAGCG ATACAGTATC TGATCCTGAG 
TGTACCACAG ACTCTTCTTC CAGCAAAGAA CACACACTGC CTTCTGTTAC CCAAGGGGGA 
ATAGAAATAA TTCTAACTGA AGAAATGAAG TTAACTGACC TGGAACTTGG TAAAATTGCC 
AACAGTATAC AGGAGTTACT GTATGATGCC TCTGACATCT GCCATGACCG CTGCGTCAAG 
TTCCTGCTAG CGAGGGCCAA GGAAGGGTTT CTTGAGAAGC TAAACTCTGC TGAATTTGTG 
TCCCTCTCAC GGGCAGTGGA AGCATTCGTT TTGGGTACTG AAAAGATCTG TGGCCGAAGG 
AGCATGTCAC TGAGAGGGGC ACTGCAAAGC CAAGCCAACA AATTTGTAAA TCGGTTCCAT 
GATGAAAGAA AAACCAAACT GAGCCTCCTG CTTGATAATG AACGCTGGAA ACAAGCTGAT 
GTCCCTGCTG AATTTCAGGA CTTAGTAGAT TCTATAAAGG ACGGTAAAAT AGTGCTTCCT 
GAGCGAAAAT CTGGAGGTAC AGAAGAAAGG AAACCCACAG ATTTTCTTAC AGTGGACGGA 
CAGAAGTATG CAGTTGTTGG CACAGTGCTA CTTTTGATCA GAATCATTCT CGAGTACTGC 
AGCTGCGTAG ATGATATCCC CTCTGTAACT ACAGACATGT TGACTCGGCT GTCTGATCTT 
TTAAAGTATT TTAATTCACG GAGTTGCCAA TTGGTTCTGG GAGCAGGAGC ATTGCAAGTG 
GTTGGCCTGA AGACAATAAC CACCAAGAAC TTAGCCTTGT CTTCGCGGTG CTTGCAGCTT 
ATAGTACACT ACATTCCCCT CATAAGGGCC CACTTTGAAG CTCGGATGCA GCCTAAGCAG 
TATAGCATGC TCCGGCATTT TGACCATATA ACCAAGGACT ACAATGACCA CATTGCAGAG 
ATTTCTGCCA AGCTTGTCAC AATAATGGAT GGCCTGTTTG AAAAGCTACT TTCCAAGTAT 
GAGGTAAAAG CCCCAGTTCC TTCTACGTGT TTCAGAACCA TCTGCAAGCA AATGGCCAAG 
ATGCACGAGG CCATTTATGA CCTCCTTCCT GAAGAGCAAA CGCAGACTCT GTTTCTTAAA 
ATAAATTCAA GCTACATATT CCATTTGAGG CGACAATTGT GTCATTTAAA TGTCATTAAT 
GATGGAGGAC CACAATACGG ATTGGTGGCA GCTGACATTG CCTTTTACAC TGAGAACGTA 
CAAGCCTTGA AAGGCCTGAA AGATCTGGAT CTTAGCTTGG CTGAAATTTG GGAGCAGAAA 
AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002939365.2
CDS	459..3404
Translation	MYAMASSHSSSLVPHSNSNKAFYKKGLQPAQQVGMVRSLPDVCPKEPTGDTKSLRDEPSVVADQHKWTIYNSKVNLPAALNDPRLAKRESDFFTKTWGQDFVESEVLPSPYLPAITKEQFLSYSQQTISREKNHEKCKSVCPLKVDFDRPAYSHVLKSRADLEQVPKIFMKPDFALEDSLTFNAVLPWSHFSSAGGKGSRDAASSKLLQEKLSHYLDIVEVNIAHQISLRSEAFFHAMTSQHELQDYLRKTSQAVKMLRDKISQIDRVMCEGSLRILRQALTRNNCIKIYNKLKLMATVHQTQPTVQLLLSTSEYVGALDLIATTQEVLQQELQGIHSFRHLSSQLCEMEKLIDKMIISEFTSYARSDLNRPLEEDSHVVEEERLVSLVFGMLKQRKLDFFEIYSDEMMATAKNIIKQCVVNTVSQIEEIDSEVVTKLTDQIRMMNFSQWFGLLQNIFSKFTIFIERVKATLSVIRNVVLMVLDKNQRVREMEMLAAQQNLRRESNVDNELAYLTHEGLFISDAFGEGELPNAATSTNFQRNTSPSSSEPSGSDTVSDPECTTDSSSSKEHTLPSVTQGGIEIILTEEMKLTDLELGKIANSIQELLYDASDICHDRCVKFLLARAKEGFLEKLNSAEFVSLSRAVEAFVLGTEKICGRRSMSLRGALQSQANKFVNRFHDERKTKLSLLLDNERWKQADVPAEFQDLVDSIKDGKIVLPERKSGGTEERKPTDFLTVDGQKYAVVGTVLLLIRIILEYCSCVDDIPSVTTDMLTRLSDLLKYFNSRSCQLVLGAGALQVVGLKTITTKNLALSSRCLQLIVHYIPLIRAHFEARMQPKQYSMLRHFDHITKDYNDHIAEISAKLVTIMDGLFEKLLSKYEVKAPVPSTCFRTICKQMAKMHEAIYDLLPEEQTQTLFLKINSSYIFHLRRQLCHLNVINDGGPQYGLVAADIAFYTENVQALKGLKDLDLSLAEIWEQKR

Target ORF information:

RefSeq Version	XM_002939319.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis VPS54 GARP complex subunit (vps54), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002939319.4

ORF Insert Sequence:

ATGTATGCAA TGGCCTCCAG CCACAGCTCT TCACTAGTGC CTCACTCGAA CAGCAACAAA 
GCTTTTTACA AAAAGGGTCT TCAGCCTGCT CAGCAAGTGG GAATGGTGCG ATCACTGCCT 
GATGTCTGCC CCAAGGAGCC TACAGGTGAT ACTAAGAGTT TGCGTGATGA ACCATCTGTA 
GTTGCAGATC AGCACAAATG GACAATTTAT AATTCTAAAG TAAACCTTCC GGCAGCATTA 
AATGACCCCC GCCTTGCAAA ACGGGAATCA GACTTTTTCA CAAAAACATG GGGGCAAGAC 
TTTGTGGAAA GTGAAGTCCT ACCATCACCC TACCTCCCAG CAATTACTAA GGAACAGTTT 
CTATCATATT CGCAGCAAAC CATCAGTAGA GAAAAAAATC ATGAAAAATG TAAGAGTGTT 
TGCCCACTAA AGGTGGACTT TGACAGACCA GCCTATTCTC ATGTTCTCAA ATCGAGAGCA 
GATCTGGAAC AAGTACCTAA GATCTTTATG AAGCCAGACT TTGCCTTGGA GGACTCCTTA 
ACATTTAATG CTGTTTTGCC ATGGTCTCAT TTTAGCAGTG CCGGAGGGAA AGGAAGCAGA 
GATGCAGCAT CCTCGAAATT ACTTCAAGAA AAGCTAAGCC ACTATTTGGA TATTGTTGAG 
GTTAACATCG CTCACCAAAT CTCCTTGCGC TCTGAAGCAT TTTTCCATGC TATGACCTCT 
CAACATGAGC TGCAGGACTA CCTCAGGAAG ACTTCCCAGG CTGTTAAAAT GCTGCGAGAC 
AAGATCTCTC AGATAGACAG AGTCATGTGT GAGGGATCTC TGCGGATCTT ACGGCAAGCA 
CTGACCAGGA ACAACTGCAT TAAAATATAC AATAAGCTCA AGTTAATGGC AACAGTGCAT 
CAAACTCAGC CTACTGTACA GCTTCTATTA TCAACCTCTG AGTATGTAGG AGCTTTGGAC 
TTAATAGCAA CGACACAAGA GGTCTTGCAG CAGGAGTTAC AGGGTATTCA CAGTTTTCGA 
CATCTCAGCT CACAACTTTG CGAAATGGAA AAACTCATTG ATAAAATGAT CATCTCTGAA 
TTTACTTCGT ATGCTCGCAG CGATTTAAAC AGACCTTTAG AAGAAGACAG TCATGTAGTG 
GAAGAGGAAA GACTGGTCTC TCTCGTATTT GGGATGTTAA AGCAGCGAAA ACTGGATTTC 
TTTGAGATTT ACTCAGACGA AATGATGGCA ACAGCAAAAA ATATCATCAA GCAGTGTGTG 
GTTAACACTG TGTCTCAGAT TGAGGAAATA GACTCAGAAG TTGTTACAAA GCTTACTGAT 
CAGATTCGGA TGATGAATTT TTCTCAGTGG TTTGGCTTGC TTCAGAATAT TTTTTCTAAG 
TTTACAATTT TTATTGAGAG AGTTAAGGCA ACGCTAAGCG TCATCCGTAA TGTAGTGCTC 
ATGGTTTTAG ACAAAAATCA GCGGGTGAGG GAAATGGAAA TGTTGGCAGC TCAACAGAAT 
CTCAGAAGAG AAAGCAATGT GGACAATGAA TTGGCGTACT TGACTCATGA AGGCTTATTT 
ATAAGTGATG CTTTCGGTGA AGGTGAGCTT CCCAATGCTG CTACCAGCAC TAACTTTCAA 
AGGAACACAT CACCCAGCAG TAGCGAGCCC TCTGGCAGCG ATACAGTATC TGATCCTGAG 
TGTACCACAG ACTCTTCTTC CAGCAAAGAA CACACACTGC CTTCTGTTAC CCAAGGGGGA 
ATAGAAATAA TTCTAACTGA AGAAATGAAG TTAACTGACC TGGAACTTGG TAAAATTGCC 
AACAGTATAC AGGAGTTACT GTATGATGCC TCTGACATCT GCCATGACCG CTGCGTCAAG 
TTCCTGCTAG CGAGGGCCAA GGAAGGGTTT CTTGAGAAGC TAAACTCTGC TGAATTTGTG 
TCCCTCTCAC GGGCAGTGGA AGCATTCGTT TTGGGTACTG AAAAGATCTG TGGCCGAAGG 
AGCATGTCAC TGAGAGGGGC ACTGCAAAGC CAAGCCAACA AATTTGTAAA TCGGTTCCAT 
GATGAAAGAA AAACCAAACT GAGCCTCCTG CTTGATAATG AACGCTGGAA ACAAGCTGAT 
GTCCCTGCTG AATTTCAGGA CTTAGTAGAT TCTATAAAGG ACGGTAAAAT AGTGCTTCCT 
GAGCGAAAAT CTGGAGGTAC AGAAGAAAGG AAACCCACAG ATTTTCTTAC AGTGGACGGA 
CAGAAGTATG CAGTTGTTGG CACAGTGCTA CTTTTGATCA GAATCATTCT CGAGTACTGC 
AGCTGCGTAG ATGATATCCC CTCTGTAACT ACAGACATGT TGACTCGGCT GTCTGATCTT 
TTAAAGTATT TTAATTCACG GAGTTGCCAA TTGGTTCTGG GAGCAGGAGC ATTGCAAGTG 
GTTGGCCTGA AGACAATAAC CACCAAGAAC TTAGCCTTGT CTTCGCGGTG CTTGCAGCTT 
ATAGTACACT ACATTCCCCT CATAAGGGCC CACTTTGAAG CTCGGATGCA GCCTAAGCAG 
TATAGCATGC TCCGGCATTT TGACCATATA ACCAAGGACT ACAATGACCA CATTGCAGAG 
ATTTCTGCCA AGCTTGTCAC AATAATGGAT GGCCTGTTTG AAAAGCTACT TTCCAAGTAT 
GAGGTAAAAG CCCCAGTTCC TTCTACGTGT TTCAGAACCA TCTGCAAGCA AATGGCCAAG 
ATGCACGAGG CCATTTATGA CCTCCTTCCT GAAGAGCAAA CGCAGACTCT GTTTCTTAAA 
ATAAATTCAA GCTACATATT CCATTTGAGG CGACAATTGT GTCATTTAAA TGTCATTAAT 
GATGGAGGAC CACAATACGG ATTGGTGGCA GCTGACATTG CCTTTTACAC TGAGAACGTA 
CAAGCCTTGA AAGGCCTGAA AGATCTGGAT CTTAGCTTGG CTGAAATTTG GGAGCAGAAA 
AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vps54 ( XM_004914708.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914765.1 Xenopus tropicalis VPS54 GARP complex subunit (vps54), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa10788
Clone ID Related Accession (Same CDS sequence)	XM_004914708.4 , XM_002939319.4 , XM_004914708.3 , XM_002939319.5
Accession Version	XM_004914708.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2946bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	vacuolar protein sorting-associated protein 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004914708.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGCAA TGGCCTCCAG CCACAGCTCT TCACTAGTGC CTCACTCGAA CAGCAACAAA 
GCTTTTTACA AAAAGGGTCT TCAGCCTGCT CAGCAAGTGG GAATGGTGCG ATCACTGCCT 
GATGTCTGCC CCAAGGAGCC TACAGGTGAT ACTAAGAGTT TGCGTGATGA ACCATCTGTA 
GTTGCAGATC AGCACAAATG GACAATTTAT AATTCTAAAG TAAACCTTCC GGCAGCATTA 
AATGACCCCC GCCTTGCAAA ACGGGAATCA GACTTTTTCA CAAAAACATG GGGGCAAGAC 
TTTGTGGAAA GTGAAGTCCT ACCATCACCC TACCTCCCAG CAATTACTAA GGAACAGTTT 
CTATCATATT CGCAGCAAAC CATCAGTAGA GAAAAAAATC ATGAAAAATG TAAGAGTGTT 
TGCCCACTAA AGGTGGACTT TGACAGACCA GCCTATTCTC ATGTTCTCAA ATCGAGAGCA 
GATCTGGAAC AAGTACCTAA GATCTTTATG AAGCCAGACT TTGCCTTGGA GGACTCCTTA 
ACATTTAATG CTGTTTTGCC ATGGTCTCAT TTTAGCAGTG CCGGAGGGAA AGGAAGCAGA 
GATGCAGCAT CCTCGAAATT ACTTCAAGAA AAGCTAAGCC ACTATTTGGA TATTGTTGAG 
GTTAACATCG CTCACCAAAT CTCCTTGCGC TCTGAAGCAT TTTTCCATGC TATGACCTCT 
CAACATGAGC TGCAGGACTA CCTCAGGAAG ACTTCCCAGG CTGTTAAAAT GCTGCGAGAC 
AAGATCTCTC AGATAGACAG AGTCATGTGT GAGGGATCTC TGCGGATCTT ACGGCAAGCA 
CTGACCAGGA ACAACTGCAT TAAAATATAC AATAAGCTCA AGTTAATGGC AACAGTGCAT 
CAAACTCAGC CTACTGTACA GCTTCTATTA TCAACCTCTG AGTATGTAGG AGCTTTGGAC 
TTAATAGCAA CGACACAAGA GGTCTTGCAG CAGGAGTTAC AGGGTATTCA CAGTTTTCGA 
CATCTCAGCT CACAACTTTG CGAAATGGAA AAACTCATTG ATAAAATGAT CATCTCTGAA 
TTTACTTCGT ATGCTCGCAG CGATTTAAAC AGACCTTTAG AAGAAGACAG TCATGTAGTG 
GAAGAGGAAA GACTGGTCTC TCTCGTATTT GGGATGTTAA AGCAGCGAAA ACTGGATTTC 
TTTGAGATTT ACTCAGACGA AATGATGGCA ACAGCAAAAA ATATCATCAA GCAGTGTGTG 
GTTAACACTG TGTCTCAGAT TGAGGAAATA GACTCAGAAG TTGTTACAAA GCTTACTGAT 
CAGATTCGGA TGATGAATTT TTCTCAGTGG TTTGGCTTGC TTCAGAATAT TTTTTCTAAG 
TTTACAATTT TTATTGAGAG AGTTAAGGCA ACGCTAAGCG TCATCCGTAA TGTAGTGCTC 
ATGGTTTTAG ACAAAAATCA GCGGGTGAGG GAAATGGAAA TGTTGGCAGC TCAACAGAAT 
CTCAGAAGAG AAAGCAATGT GGACAATGAA TTGGCGTACT TGACTCATGA AGGCTTATTT 
ATAAGTGATG CTTTCGGTGA AGGTGAGCTT CCCAATGCTG CTACCAGCAC TAACTTTCAA 
AGGAACACAT CACCCAGCAG TAGCGAGCCC TCTGGCAGCG ATACAGTATC TGATCCTGAG 
TGTACCACAG ACTCTTCTTC CAGCAAAGAA CACACACTGC CTTCTGTTAC CCAAGGGGGA 
ATAGAAATAA TTCTAACTGA AGAAATGAAG TTAACTGACC TGGAACTTGG TAAAATTGCC 
AACAGTATAC AGGAGTTACT GTATGATGCC TCTGACATCT GCCATGACCG CTGCGTCAAG 
TTCCTGCTAG CGAGGGCCAA GGAAGGGTTT CTTGAGAAGC TAAACTCTGC TGAATTTGTG 
TCCCTCTCAC GGGCAGTGGA AGCATTCGTT TTGGGTACTG AAAAGATCTG TGGCCGAAGG 
AGCATGTCAC TGAGAGGGGC ACTGCAAAGC CAAGCCAACA AATTTGTAAA TCGGTTCCAT 
GATGAAAGAA AAACCAAACT GAGCCTCCTG CTTGATAATG AACGCTGGAA ACAAGCTGAT 
GTCCCTGCTG AATTTCAGGA CTTAGTAGAT TCTATAAAGG ACGGTAAAAT AGTGCTTCCT 
GAGCGAAAAT CTGGAGGTAC AGAAGAAAGG AAACCCACAG ATTTTCTTAC AGTGGACGGA 
CAGAAGTATG CAGTTGTTGG CACAGTGCTA CTTTTGATCA GAATCATTCT CGAGTACTGC 
AGCTGCGTAG ATGATATCCC CTCTGTAACT ACAGACATGT TGACTCGGCT GTCTGATCTT 
TTAAAGTATT TTAATTCACG GAGTTGCCAA TTGGTTCTGG GAGCAGGAGC ATTGCAAGTG 
GTTGGCCTGA AGACAATAAC CACCAAGAAC TTAGCCTTGT CTTCGCGGTG CTTGCAGCTT 
ATAGTACACT ACATTCCCCT CATAAGGGCC CACTTTGAAG CTCGGATGCA GCCTAAGCAG 
TATAGCATGC TCCGGCATTT TGACCATATA ACCAAGGACT ACAATGACCA CATTGCAGAG 
ATTTCTGCCA AGCTTGTCAC AATAATGGAT GGCCTGTTTG AAAAGCTACT TTCCAAGTAT 
GAGGTAAAAG CCCCAGTTCC TTCTACGTGT TTCAGAACCA TCTGCAAGCA AATGGCCAAG 
ATGCACGAGG CCATTTATGA CCTCCTTCCT GAAGAGCAAA CGCAGACTCT GTTTCTTAAA 
ATAAATTCAA GCTACATATT CCATTTGAGG CGACAATTGT GTCATTTAAA TGTCATTAAT 
GATGGAGGAC CACAATACGG ATTGGTGGCA GCTGACATTG CCTTTTACAC TGAGAACGTA 
CAAGCCTTGA AAGGCCTGAA AGATCTGGAT CTTAGCTTGG CTGAAATTTG GGAGCAGAAA 
AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914765.1
CDS	346..3291
Translation	MYAMASSHSSSLVPHSNSNKAFYKKGLQPAQQVGMVRSLPDVCPKEPTGDTKSLRDEPSVVADQHKWTIYNSKVNLPAALNDPRLAKRESDFFTKTWGQDFVESEVLPSPYLPAITKEQFLSYSQQTISREKNHEKCKSVCPLKVDFDRPAYSHVLKSRADLEQVPKIFMKPDFALEDSLTFNAVLPWSHFSSAGGKGSRDAASSKLLQEKLSHYLDIVEVNIAHQISLRSEAFFHAMTSQHELQDYLRKTSQAVKMLRDKISQIDRVMCEGSLRILRQALTRNNCIKIYNKLKLMATVHQTQPTVQLLLSTSEYVGALDLIATTQEVLQQELQGIHSFRHLSSQLCEMEKLIDKMIISEFTSYARSDLNRPLEEDSHVVEEERLVSLVFGMLKQRKLDFFEIYSDEMMATAKNIIKQCVVNTVSQIEEIDSEVVTKLTDQIRMMNFSQWFGLLQNIFSKFTIFIERVKATLSVIRNVVLMVLDKNQRVREMEMLAAQQNLRRESNVDNELAYLTHEGLFISDAFGEGELPNAATSTNFQRNTSPSSSEPSGSDTVSDPECTTDSSSSKEHTLPSVTQGGIEIILTEEMKLTDLELGKIANSIQELLYDASDICHDRCVKFLLARAKEGFLEKLNSAEFVSLSRAVEAFVLGTEKICGRRSMSLRGALQSQANKFVNRFHDERKTKLSLLLDNERWKQADVPAEFQDLVDSIKDGKIVLPERKSGGTEERKPTDFLTVDGQKYAVVGTVLLLIRIILEYCSCVDDIPSVTTDMLTRLSDLLKYFNSRSCQLVLGAGALQVVGLKTITTKNLALSSRCLQLIVHYIPLIRAHFEARMQPKQYSMLRHFDHITKDYNDHIAEISAKLVTIMDGLFEKLLSKYEVKAPVPSTCFRTICKQMAKMHEAIYDLLPEEQTQTLFLKINSSYIFHLRRQLCHLNVINDGGPQYGLVAADIAFYTENVQALKGLKDLDLSLAEIWEQKR

Target ORF information:

RefSeq Version	XM_004914708.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis VPS54 GARP complex subunit (vps54), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914708.3

ORF Insert Sequence:

ATGTATGCAA TGGCCTCCAG CCACAGCTCT TCACTAGTGC CTCACTCGAA CAGCAACAAA 
GCTTTTTACA AAAAGGGTCT TCAGCCTGCT CAGCAAGTGG GAATGGTGCG ATCACTGCCT 
GATGTCTGCC CCAAGGAGCC TACAGGTGAT ACTAAGAGTT TGCGTGATGA ACCATCTGTA 
GTTGCAGATC AGCACAAATG GACAATTTAT AATTCTAAAG TAAACCTTCC GGCAGCATTA 
AATGACCCCC GCCTTGCAAA ACGGGAATCA GACTTTTTCA CAAAAACATG GGGGCAAGAC 
TTTGTGGAAA GTGAAGTCCT ACCATCACCC TACCTCCCAG CAATTACTAA GGAACAGTTT 
CTATCATATT CGCAGCAAAC CATCAGTAGA GAAAAAAATC ATGAAAAATG TAAGAGTGTT 
TGCCCACTAA AGGTGGACTT TGACAGACCA GCCTATTCTC ATGTTCTCAA ATCGAGAGCA 
GATCTGGAAC AAGTACCTAA GATCTTTATG AAGCCAGACT TTGCCTTGGA GGACTCCTTA 
ACATTTAATG CTGTTTTGCC ATGGTCTCAT TTTAGCAGTG CCGGAGGGAA AGGAAGCAGA 
GATGCAGCAT CCTCGAAATT ACTTCAAGAA AAGCTAAGCC ACTATTTGGA TATTGTTGAG 
GTTAACATCG CTCACCAAAT CTCCTTGCGC TCTGAAGCAT TTTTCCATGC TATGACCTCT 
CAACATGAGC TGCAGGACTA CCTCAGGAAG ACTTCCCAGG CTGTTAAAAT GCTGCGAGAC 
AAGATCTCTC AGATAGACAG AGTCATGTGT GAGGGATCTC TGCGGATCTT ACGGCAAGCA 
CTGACCAGGA ACAACTGCAT TAAAATATAC AATAAGCTCA AGTTAATGGC AACAGTGCAT 
CAAACTCAGC CTACTGTACA GCTTCTATTA TCAACCTCTG AGTATGTAGG AGCTTTGGAC 
TTAATAGCAA CGACACAAGA GGTCTTGCAG CAGGAGTTAC AGGGTATTCA CAGTTTTCGA 
CATCTCAGCT CACAACTTTG CGAAATGGAA AAACTCATTG ATAAAATGAT CATCTCTGAA 
TTTACTTCGT ATGCTCGCAG CGATTTAAAC AGACCTTTAG AAGAAGACAG TCATGTAGTG 
GAAGAGGAAA GACTGGTCTC TCTCGTATTT GGGATGTTAA AGCAGCGAAA ACTGGATTTC 
TTTGAGATTT ACTCAGACGA AATGATGGCA ACAGCAAAAA ATATCATCAA GCAGTGTGTG 
GTTAACACTG TGTCTCAGAT TGAGGAAATA GACTCAGAAG TTGTTACAAA GCTTACTGAT 
CAGATTCGGA TGATGAATTT TTCTCAGTGG TTTGGCTTGC TTCAGAATAT TTTTTCTAAG 
TTTACAATTT TTATTGAGAG AGTTAAGGCA ACGCTAAGCG TCATCCGTAA TGTAGTGCTC 
ATGGTTTTAG ACAAAAATCA GCGGGTGAGG GAAATGGAAA TGTTGGCAGC TCAACAGAAT 
CTCAGAAGAG AAAGCAATGT GGACAATGAA TTGGCGTACT TGACTCATGA AGGCTTATTT 
ATAAGTGATG CTTTCGGTGA AGGTGAGCTT CCCAATGCTG CTACCAGCAC TAACTTTCAA 
AGGAACACAT CACCCAGCAG TAGCGAGCCC TCTGGCAGCG ATACAGTATC TGATCCTGAG 
TGTACCACAG ACTCTTCTTC CAGCAAAGAA CACACACTGC CTTCTGTTAC CCAAGGGGGA 
ATAGAAATAA TTCTAACTGA AGAAATGAAG TTAACTGACC TGGAACTTGG TAAAATTGCC 
AACAGTATAC AGGAGTTACT GTATGATGCC TCTGACATCT GCCATGACCG CTGCGTCAAG 
TTCCTGCTAG CGAGGGCCAA GGAAGGGTTT CTTGAGAAGC TAAACTCTGC TGAATTTGTG 
TCCCTCTCAC GGGCAGTGGA AGCATTCGTT TTGGGTACTG AAAAGATCTG TGGCCGAAGG 
AGCATGTCAC TGAGAGGGGC ACTGCAAAGC CAAGCCAACA AATTTGTAAA TCGGTTCCAT 
GATGAAAGAA AAACCAAACT GAGCCTCCTG CTTGATAATG AACGCTGGAA ACAAGCTGAT 
GTCCCTGCTG AATTTCAGGA CTTAGTAGAT TCTATAAAGG ACGGTAAAAT AGTGCTTCCT 
GAGCGAAAAT CTGGAGGTAC AGAAGAAAGG AAACCCACAG ATTTTCTTAC AGTGGACGGA 
CAGAAGTATG CAGTTGTTGG CACAGTGCTA CTTTTGATCA GAATCATTCT CGAGTACTGC 
AGCTGCGTAG ATGATATCCC CTCTGTAACT ACAGACATGT TGACTCGGCT GTCTGATCTT 
TTAAAGTATT TTAATTCACG GAGTTGCCAA TTGGTTCTGG GAGCAGGAGC ATTGCAAGTG 
GTTGGCCTGA AGACAATAAC CACCAAGAAC TTAGCCTTGT CTTCGCGGTG CTTGCAGCTT 
ATAGTACACT ACATTCCCCT CATAAGGGCC CACTTTGAAG CTCGGATGCA GCCTAAGCAG 
TATAGCATGC TCCGGCATTT TGACCATATA ACCAAGGACT ACAATGACCA CATTGCAGAG 
ATTTCTGCCA AGCTTGTCAC AATAATGGAT GGCCTGTTTG AAAAGCTACT TTCCAAGTAT 
GAGGTAAAAG CCCCAGTTCC TTCTACGTGT TTCAGAACCA TCTGCAAGCA AATGGCCAAG 
ATGCACGAGG CCATTTATGA CCTCCTTCCT GAAGAGCAAA CGCAGACTCT GTTTCTTAAA 
ATAAATTCAA GCTACATATT CCATTTGAGG CGACAATTGT GTCATTTAAA TGTCATTAAT 
GATGGAGGAC CACAATACGG ATTGGTGGCA GCTGACATTG CCTTTTACAC TGAGAACGTA 
CAAGCCTTGA AAGGCCTGAA AGATCTGGAT CTTAGCTTGG CTGAAATTTG GGAGCAGAAA 
AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vps54 ( XM_004914708.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914765.1 Xenopus tropicalis VPS54 GARP complex subunit (vps54), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa10788
Clone ID Related Accession (Same CDS sequence)	XM_004914708.4 , XM_002939319.4 , XM_004914708.3 , XM_002939319.5
Accession Version	XM_004914708.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2946bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	vacuolar protein sorting-associated protein 54
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004914708.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGCAA TGGCCTCCAG CCACAGCTCT TCACTAGTGC CTCACTCGAA CAGCAACAAA 
GCTTTTTACA AAAAGGGTCT TCAGCCTGCT CAGCAAGTGG GAATGGTGCG ATCACTGCCT 
GATGTCTGCC CCAAGGAGCC TACAGGTGAT ACTAAGAGTT TGCGTGATGA ACCATCTGTA 
GTTGCAGATC AGCACAAATG GACAATTTAT AATTCTAAAG TAAACCTTCC GGCAGCATTA 
AATGACCCCC GCCTTGCAAA ACGGGAATCA GACTTTTTCA CAAAAACATG GGGGCAAGAC 
TTTGTGGAAA GTGAAGTCCT ACCATCACCC TACCTCCCAG CAATTACTAA GGAACAGTTT 
CTATCATATT CGCAGCAAAC CATCAGTAGA GAAAAAAATC ATGAAAAATG TAAGAGTGTT 
TGCCCACTAA AGGTGGACTT TGACAGACCA GCCTATTCTC ATGTTCTCAA ATCGAGAGCA 
GATCTGGAAC AAGTACCTAA GATCTTTATG AAGCCAGACT TTGCCTTGGA GGACTCCTTA 
ACATTTAATG CTGTTTTGCC ATGGTCTCAT TTTAGCAGTG CCGGAGGGAA AGGAAGCAGA 
GATGCAGCAT CCTCGAAATT ACTTCAAGAA AAGCTAAGCC ACTATTTGGA TATTGTTGAG 
GTTAACATCG CTCACCAAAT CTCCTTGCGC TCTGAAGCAT TTTTCCATGC TATGACCTCT 
CAACATGAGC TGCAGGACTA CCTCAGGAAG ACTTCCCAGG CTGTTAAAAT GCTGCGAGAC 
AAGATCTCTC AGATAGACAG AGTCATGTGT GAGGGATCTC TGCGGATCTT ACGGCAAGCA 
CTGACCAGGA ACAACTGCAT TAAAATATAC AATAAGCTCA AGTTAATGGC AACAGTGCAT 
CAAACTCAGC CTACTGTACA GCTTCTATTA TCAACCTCTG AGTATGTAGG AGCTTTGGAC 
TTAATAGCAA CGACACAAGA GGTCTTGCAG CAGGAGTTAC AGGGTATTCA CAGTTTTCGA 
CATCTCAGCT CACAACTTTG CGAAATGGAA AAACTCATTG ATAAAATGAT CATCTCTGAA 
TTTACTTCGT ATGCTCGCAG CGATTTAAAC AGACCTTTAG AAGAAGACAG TCATGTAGTG 
GAAGAGGAAA GACTGGTCTC TCTCGTATTT GGGATGTTAA AGCAGCGAAA ACTGGATTTC 
TTTGAGATTT ACTCAGACGA AATGATGGCA ACAGCAAAAA ATATCATCAA GCAGTGTGTG 
GTTAACACTG TGTCTCAGAT TGAGGAAATA GACTCAGAAG TTGTTACAAA GCTTACTGAT 
CAGATTCGGA TGATGAATTT TTCTCAGTGG TTTGGCTTGC TTCAGAATAT TTTTTCTAAG 
TTTACAATTT TTATTGAGAG AGTTAAGGCA ACGCTAAGCG TCATCCGTAA TGTAGTGCTC 
ATGGTTTTAG ACAAAAATCA GCGGGTGAGG GAAATGGAAA TGTTGGCAGC TCAACAGAAT 
CTCAGAAGAG AAAGCAATGT GGACAATGAA TTGGCGTACT TGACTCATGA AGGCTTATTT 
ATAAGTGATG CTTTCGGTGA AGGTGAGCTT CCCAATGCTG CTACCAGCAC TAACTTTCAA 
AGGAACACAT CACCCAGCAG TAGCGAGCCC TCTGGCAGCG ATACAGTATC TGATCCTGAG 
TGTACCACAG ACTCTTCTTC CAGCAAAGAA CACACACTGC CTTCTGTTAC CCAAGGGGGA 
ATAGAAATAA TTCTAACTGA AGAAATGAAG TTAACTGACC TGGAACTTGG TAAAATTGCC 
AACAGTATAC AGGAGTTACT GTATGATGCC TCTGACATCT GCCATGACCG CTGCGTCAAG 
TTCCTGCTAG CGAGGGCCAA GGAAGGGTTT CTTGAGAAGC TAAACTCTGC TGAATTTGTG 
TCCCTCTCAC GGGCAGTGGA AGCATTCGTT TTGGGTACTG AAAAGATCTG TGGCCGAAGG 
AGCATGTCAC TGAGAGGGGC ACTGCAAAGC CAAGCCAACA AATTTGTAAA TCGGTTCCAT 
GATGAAAGAA AAACCAAACT GAGCCTCCTG CTTGATAATG AACGCTGGAA ACAAGCTGAT 
GTCCCTGCTG AATTTCAGGA CTTAGTAGAT TCTATAAAGG ACGGTAAAAT AGTGCTTCCT 
GAGCGAAAAT CTGGAGGTAC AGAAGAAAGG AAACCCACAG ATTTTCTTAC AGTGGACGGA 
CAGAAGTATG CAGTTGTTGG CACAGTGCTA CTTTTGATCA GAATCATTCT CGAGTACTGC 
AGCTGCGTAG ATGATATCCC CTCTGTAACT ACAGACATGT TGACTCGGCT GTCTGATCTT 
TTAAAGTATT TTAATTCACG GAGTTGCCAA TTGGTTCTGG GAGCAGGAGC ATTGCAAGTG 
GTTGGCCTGA AGACAATAAC CACCAAGAAC TTAGCCTTGT CTTCGCGGTG CTTGCAGCTT 
ATAGTACACT ACATTCCCCT CATAAGGGCC CACTTTGAAG CTCGGATGCA GCCTAAGCAG 
TATAGCATGC TCCGGCATTT TGACCATATA ACCAAGGACT ACAATGACCA CATTGCAGAG 
ATTTCTGCCA AGCTTGTCAC AATAATGGAT GGCCTGTTTG AAAAGCTACT TTCCAAGTAT 
GAGGTAAAAG CCCCAGTTCC TTCTACGTGT TTCAGAACCA TCTGCAAGCA AATGGCCAAG 
ATGCACGAGG CCATTTATGA CCTCCTTCCT GAAGAGCAAA CGCAGACTCT GTTTCTTAAA 
ATAAATTCAA GCTACATATT CCATTTGAGG CGACAATTGT GTCATTTAAA TGTCATTAAT 
GATGGAGGAC CACAATACGG ATTGGTGGCA GCTGACATTG CCTTTTACAC TGAGAACGTA 
CAAGCCTTGA AAGGCCTGAA AGATCTGGAT CTTAGCTTGG CTGAAATTTG GGAGCAGAAA 
AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914765.1
CDS	261..3206
Translation	MYAMASSHSSSLVPHSNSNKAFYKKGLQPAQQVGMVRSLPDVCPKEPTGDTKSLRDEPSVVADQHKWTIYNSKVNLPAALNDPRLAKRESDFFTKTWGQDFVESEVLPSPYLPAITKEQFLSYSQQTISREKNHEKCKSVCPLKVDFDRPAYSHVLKSRADLEQVPKIFMKPDFALEDSLTFNAVLPWSHFSSAGGKGSRDAASSKLLQEKLSHYLDIVEVNIAHQISLRSEAFFHAMTSQHELQDYLRKTSQAVKMLRDKISQIDRVMCEGSLRILRQALTRNNCIKIYNKLKLMATVHQTQPTVQLLLSTSEYVGALDLIATTQEVLQQELQGIHSFRHLSSQLCEMEKLIDKMIISEFTSYARSDLNRPLEEDSHVVEEERLVSLVFGMLKQRKLDFFEIYSDEMMATAKNIIKQCVVNTVSQIEEIDSEVVTKLTDQIRMMNFSQWFGLLQNIFSKFTIFIERVKATLSVIRNVVLMVLDKNQRVREMEMLAAQQNLRRESNVDNELAYLTHEGLFISDAFGEGELPNAATSTNFQRNTSPSSSEPSGSDTVSDPECTTDSSSSKEHTLPSVTQGGIEIILTEEMKLTDLELGKIANSIQELLYDASDICHDRCVKFLLARAKEGFLEKLNSAEFVSLSRAVEAFVLGTEKICGRRSMSLRGALQSQANKFVNRFHDERKTKLSLLLDNERWKQADVPAEFQDLVDSIKDGKIVLPERKSGGTEERKPTDFLTVDGQKYAVVGTVLLLIRIILEYCSCVDDIPSVTTDMLTRLSDLLKYFNSRSCQLVLGAGALQVVGLKTITTKNLALSSRCLQLIVHYIPLIRAHFEARMQPKQYSMLRHFDHITKDYNDHIAEISAKLVTIMDGLFEKLLSKYEVKAPVPSTCFRTICKQMAKMHEAIYDLLPEEQTQTLFLKINSSYIFHLRRQLCHLNVINDGGPQYGLVAADIAFYTENVQALKGLKDLDLSLAEIWEQKR

Target ORF information:

RefSeq Version	XM_004914708.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis VPS54 GARP complex subunit (vps54), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914708.4

ORF Insert Sequence:

ATGTATGCAA TGGCCTCCAG CCACAGCTCT TCACTAGTGC CTCACTCGAA CAGCAACAAA 
GCTTTTTACA AAAAGGGTCT TCAGCCTGCT CAGCAAGTGG GAATGGTGCG ATCACTGCCT 
GATGTCTGCC CCAAGGAGCC TACAGGTGAT ACTAAGAGTT TGCGTGATGA ACCATCTGTA 
GTTGCAGATC AGCACAAATG GACAATTTAT AATTCTAAAG TAAACCTTCC GGCAGCATTA 
AATGACCCCC GCCTTGCAAA ACGGGAATCA GACTTTTTCA CAAAAACATG GGGGCAAGAC 
TTTGTGGAAA GTGAAGTCCT ACCATCACCC TACCTCCCAG CAATTACTAA GGAACAGTTT 
CTATCATATT CGCAGCAAAC CATCAGTAGA GAAAAAAATC ATGAAAAATG TAAGAGTGTT 
TGCCCACTAA AGGTGGACTT TGACAGACCA GCCTATTCTC ATGTTCTCAA ATCGAGAGCA 
GATCTGGAAC AAGTACCTAA GATCTTTATG AAGCCAGACT TTGCCTTGGA GGACTCCTTA 
ACATTTAATG CTGTTTTGCC ATGGTCTCAT TTTAGCAGTG CCGGAGGGAA AGGAAGCAGA 
GATGCAGCAT CCTCGAAATT ACTTCAAGAA AAGCTAAGCC ACTATTTGGA TATTGTTGAG 
GTTAACATCG CTCACCAAAT CTCCTTGCGC TCTGAAGCAT TTTTCCATGC TATGACCTCT 
CAACATGAGC TGCAGGACTA CCTCAGGAAG ACTTCCCAGG CTGTTAAAAT GCTGCGAGAC 
AAGATCTCTC AGATAGACAG AGTCATGTGT GAGGGATCTC TGCGGATCTT ACGGCAAGCA 
CTGACCAGGA ACAACTGCAT TAAAATATAC AATAAGCTCA AGTTAATGGC AACAGTGCAT 
CAAACTCAGC CTACTGTACA GCTTCTATTA TCAACCTCTG AGTATGTAGG AGCTTTGGAC 
TTAATAGCAA CGACACAAGA GGTCTTGCAG CAGGAGTTAC AGGGTATTCA CAGTTTTCGA 
CATCTCAGCT CACAACTTTG CGAAATGGAA AAACTCATTG ATAAAATGAT CATCTCTGAA 
TTTACTTCGT ATGCTCGCAG CGATTTAAAC AGACCTTTAG AAGAAGACAG TCATGTAGTG 
GAAGAGGAAA GACTGGTCTC TCTCGTATTT GGGATGTTAA AGCAGCGAAA ACTGGATTTC 
TTTGAGATTT ACTCAGACGA AATGATGGCA ACAGCAAAAA ATATCATCAA GCAGTGTGTG 
GTTAACACTG TGTCTCAGAT TGAGGAAATA GACTCAGAAG TTGTTACAAA GCTTACTGAT 
CAGATTCGGA TGATGAATTT TTCTCAGTGG TTTGGCTTGC TTCAGAATAT TTTTTCTAAG 
TTTACAATTT TTATTGAGAG AGTTAAGGCA ACGCTAAGCG TCATCCGTAA TGTAGTGCTC 
ATGGTTTTAG ACAAAAATCA GCGGGTGAGG GAAATGGAAA TGTTGGCAGC TCAACAGAAT 
CTCAGAAGAG AAAGCAATGT GGACAATGAA TTGGCGTACT TGACTCATGA AGGCTTATTT 
ATAAGTGATG CTTTCGGTGA AGGTGAGCTT CCCAATGCTG CTACCAGCAC TAACTTTCAA 
AGGAACACAT CACCCAGCAG TAGCGAGCCC TCTGGCAGCG ATACAGTATC TGATCCTGAG 
TGTACCACAG ACTCTTCTTC CAGCAAAGAA CACACACTGC CTTCTGTTAC CCAAGGGGGA 
ATAGAAATAA TTCTAACTGA AGAAATGAAG TTAACTGACC TGGAACTTGG TAAAATTGCC 
AACAGTATAC AGGAGTTACT GTATGATGCC TCTGACATCT GCCATGACCG CTGCGTCAAG 
TTCCTGCTAG CGAGGGCCAA GGAAGGGTTT CTTGAGAAGC TAAACTCTGC TGAATTTGTG 
TCCCTCTCAC GGGCAGTGGA AGCATTCGTT TTGGGTACTG AAAAGATCTG TGGCCGAAGG 
AGCATGTCAC TGAGAGGGGC ACTGCAAAGC CAAGCCAACA AATTTGTAAA TCGGTTCCAT 
GATGAAAGAA AAACCAAACT GAGCCTCCTG CTTGATAATG AACGCTGGAA ACAAGCTGAT 
GTCCCTGCTG AATTTCAGGA CTTAGTAGAT TCTATAAAGG ACGGTAAAAT AGTGCTTCCT 
GAGCGAAAAT CTGGAGGTAC AGAAGAAAGG AAACCCACAG ATTTTCTTAC AGTGGACGGA 
CAGAAGTATG CAGTTGTTGG CACAGTGCTA CTTTTGATCA GAATCATTCT CGAGTACTGC 
AGCTGCGTAG ATGATATCCC CTCTGTAACT ACAGACATGT TGACTCGGCT GTCTGATCTT 
TTAAAGTATT TTAATTCACG GAGTTGCCAA TTGGTTCTGG GAGCAGGAGC ATTGCAAGTG 
GTTGGCCTGA AGACAATAAC CACCAAGAAC TTAGCCTTGT CTTCGCGGTG CTTGCAGCTT 
ATAGTACACT ACATTCCCCT CATAAGGGCC CACTTTGAAG CTCGGATGCA GCCTAAGCAG 
TATAGCATGC TCCGGCATTT TGACCATATA ACCAAGGACT ACAATGACCA CATTGCAGAG 
ATTTCTGCCA AGCTTGTCAC AATAATGGAT GGCCTGTTTG AAAAGCTACT TTCCAAGTAT 
GAGGTAAAAG CCCCAGTTCC TTCTACGTGT TTCAGAACCA TCTGCAAGCA AATGGCCAAG 
ATGCACGAGG CCATTTATGA CCTCCTTCCT GAAGAGCAAA CGCAGACTCT GTTTCTTAAA 
ATAAATTCAA GCTACATATT CCATTTGAGG CGACAATTGT GTCATTTAAA TGTCATTAAT 
GATGGAGGAC CACAATACGG ATTGGTGGCA GCTGACATTG CCTTTTACAC TGAGAACGTA 
CAAGCCTTGA AAGGCCTGAA AGATCTGGAT CTTAGCTTGG CTGAAATTTG GGAGCAGAAA 
AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vps54 ( XM_002939319.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002939365.2 Xenopus tropicalis VPS54 GARP complex subunit (vps54), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa10788
Clone ID Related Accession (Same CDS sequence)	XM_004914708.4 , XM_002939319.4 , XM_004914708.3 , XM_002939319.5
Accession Version	XM_002939319.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2946bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	vacuolar protein sorting-associated protein 54
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002939319.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGCAA TGGCCTCCAG CCACAGCTCT TCACTAGTGC CTCACTCGAA CAGCAACAAA 
GCTTTTTACA AAAAGGGTCT TCAGCCTGCT CAGCAAGTGG GAATGGTGCG ATCACTGCCT 
GATGTCTGCC CCAAGGAGCC TACAGGTGAT ACTAAGAGTT TGCGTGATGA ACCATCTGTA 
GTTGCAGATC AGCACAAATG GACAATTTAT AATTCTAAAG TAAACCTTCC GGCAGCATTA 
AATGACCCCC GCCTTGCAAA ACGGGAATCA GACTTTTTCA CAAAAACATG GGGGCAAGAC 
TTTGTGGAAA GTGAAGTCCT ACCATCACCC TACCTCCCAG CAATTACTAA GGAACAGTTT 
CTATCATATT CGCAGCAAAC CATCAGTAGA GAAAAAAATC ATGAAAAATG TAAGAGTGTT 
TGCCCACTAA AGGTGGACTT TGACAGACCA GCCTATTCTC ATGTTCTCAA ATCGAGAGCA 
GATCTGGAAC AAGTACCTAA GATCTTTATG AAGCCAGACT TTGCCTTGGA GGACTCCTTA 
ACATTTAATG CTGTTTTGCC ATGGTCTCAT TTTAGCAGTG CCGGAGGGAA AGGAAGCAGA 
GATGCAGCAT CCTCGAAATT ACTTCAAGAA AAGCTAAGCC ACTATTTGGA TATTGTTGAG 
GTTAACATCG CTCACCAAAT CTCCTTGCGC TCTGAAGCAT TTTTCCATGC TATGACCTCT 
CAACATGAGC TGCAGGACTA CCTCAGGAAG ACTTCCCAGG CTGTTAAAAT GCTGCGAGAC 
AAGATCTCTC AGATAGACAG AGTCATGTGT GAGGGATCTC TGCGGATCTT ACGGCAAGCA 
CTGACCAGGA ACAACTGCAT TAAAATATAC AATAAGCTCA AGTTAATGGC AACAGTGCAT 
CAAACTCAGC CTACTGTACA GCTTCTATTA TCAACCTCTG AGTATGTAGG AGCTTTGGAC 
TTAATAGCAA CGACACAAGA GGTCTTGCAG CAGGAGTTAC AGGGTATTCA CAGTTTTCGA 
CATCTCAGCT CACAACTTTG CGAAATGGAA AAACTCATTG ATAAAATGAT CATCTCTGAA 
TTTACTTCGT ATGCTCGCAG CGATTTAAAC AGACCTTTAG AAGAAGACAG TCATGTAGTG 
GAAGAGGAAA GACTGGTCTC TCTCGTATTT GGGATGTTAA AGCAGCGAAA ACTGGATTTC 
TTTGAGATTT ACTCAGACGA AATGATGGCA ACAGCAAAAA ATATCATCAA GCAGTGTGTG 
GTTAACACTG TGTCTCAGAT TGAGGAAATA GACTCAGAAG TTGTTACAAA GCTTACTGAT 
CAGATTCGGA TGATGAATTT TTCTCAGTGG TTTGGCTTGC TTCAGAATAT TTTTTCTAAG 
TTTACAATTT TTATTGAGAG AGTTAAGGCA ACGCTAAGCG TCATCCGTAA TGTAGTGCTC 
ATGGTTTTAG ACAAAAATCA GCGGGTGAGG GAAATGGAAA TGTTGGCAGC TCAACAGAAT 
CTCAGAAGAG AAAGCAATGT GGACAATGAA TTGGCGTACT TGACTCATGA AGGCTTATTT 
ATAAGTGATG CTTTCGGTGA AGGTGAGCTT CCCAATGCTG CTACCAGCAC TAACTTTCAA 
AGGAACACAT CACCCAGCAG TAGCGAGCCC TCTGGCAGCG ATACAGTATC TGATCCTGAG 
TGTACCACAG ACTCTTCTTC CAGCAAAGAA CACACACTGC CTTCTGTTAC CCAAGGGGGA 
ATAGAAATAA TTCTAACTGA AGAAATGAAG TTAACTGACC TGGAACTTGG TAAAATTGCC 
AACAGTATAC AGGAGTTACT GTATGATGCC TCTGACATCT GCCATGACCG CTGCGTCAAG 
TTCCTGCTAG CGAGGGCCAA GGAAGGGTTT CTTGAGAAGC TAAACTCTGC TGAATTTGTG 
TCCCTCTCAC GGGCAGTGGA AGCATTCGTT TTGGGTACTG AAAAGATCTG TGGCCGAAGG 
AGCATGTCAC TGAGAGGGGC ACTGCAAAGC CAAGCCAACA AATTTGTAAA TCGGTTCCAT 
GATGAAAGAA AAACCAAACT GAGCCTCCTG CTTGATAATG AACGCTGGAA ACAAGCTGAT 
GTCCCTGCTG AATTTCAGGA CTTAGTAGAT TCTATAAAGG ACGGTAAAAT AGTGCTTCCT 
GAGCGAAAAT CTGGAGGTAC AGAAGAAAGG AAACCCACAG ATTTTCTTAC AGTGGACGGA 
CAGAAGTATG CAGTTGTTGG CACAGTGCTA CTTTTGATCA GAATCATTCT CGAGTACTGC 
AGCTGCGTAG ATGATATCCC CTCTGTAACT ACAGACATGT TGACTCGGCT GTCTGATCTT 
TTAAAGTATT TTAATTCACG GAGTTGCCAA TTGGTTCTGG GAGCAGGAGC ATTGCAAGTG 
GTTGGCCTGA AGACAATAAC CACCAAGAAC TTAGCCTTGT CTTCGCGGTG CTTGCAGCTT 
ATAGTACACT ACATTCCCCT CATAAGGGCC CACTTTGAAG CTCGGATGCA GCCTAAGCAG 
TATAGCATGC TCCGGCATTT TGACCATATA ACCAAGGACT ACAATGACCA CATTGCAGAG 
ATTTCTGCCA AGCTTGTCAC AATAATGGAT GGCCTGTTTG AAAAGCTACT TTCCAAGTAT 
GAGGTAAAAG CCCCAGTTCC TTCTACGTGT TTCAGAACCA TCTGCAAGCA AATGGCCAAG 
ATGCACGAGG CCATTTATGA CCTCCTTCCT GAAGAGCAAA CGCAGACTCT GTTTCTTAAA 
ATAAATTCAA GCTACATATT CCATTTGAGG CGACAATTGT GTCATTTAAA TGTCATTAAT 
GATGGAGGAC CACAATACGG ATTGGTGGCA GCTGACATTG CCTTTTACAC TGAGAACGTA 
CAAGCCTTGA AAGGCCTGAA AGATCTGGAT CTTAGCTTGG CTGAAATTTG GGAGCAGAAA 
AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002939365.2
CDS	113..3058
Translation	MYAMASSHSSSLVPHSNSNKAFYKKGLQPAQQVGMVRSLPDVCPKEPTGDTKSLRDEPSVVADQHKWTIYNSKVNLPAALNDPRLAKRESDFFTKTWGQDFVESEVLPSPYLPAITKEQFLSYSQQTISREKNHEKCKSVCPLKVDFDRPAYSHVLKSRADLEQVPKIFMKPDFALEDSLTFNAVLPWSHFSSAGGKGSRDAASSKLLQEKLSHYLDIVEVNIAHQISLRSEAFFHAMTSQHELQDYLRKTSQAVKMLRDKISQIDRVMCEGSLRILRQALTRNNCIKIYNKLKLMATVHQTQPTVQLLLSTSEYVGALDLIATTQEVLQQELQGIHSFRHLSSQLCEMEKLIDKMIISEFTSYARSDLNRPLEEDSHVVEEERLVSLVFGMLKQRKLDFFEIYSDEMMATAKNIIKQCVVNTVSQIEEIDSEVVTKLTDQIRMMNFSQWFGLLQNIFSKFTIFIERVKATLSVIRNVVLMVLDKNQRVREMEMLAAQQNLRRESNVDNELAYLTHEGLFISDAFGEGELPNAATSTNFQRNTSPSSSEPSGSDTVSDPECTTDSSSSKEHTLPSVTQGGIEIILTEEMKLTDLELGKIANSIQELLYDASDICHDRCVKFLLARAKEGFLEKLNSAEFVSLSRAVEAFVLGTEKICGRRSMSLRGALQSQANKFVNRFHDERKTKLSLLLDNERWKQADVPAEFQDLVDSIKDGKIVLPERKSGGTEERKPTDFLTVDGQKYAVVGTVLLLIRIILEYCSCVDDIPSVTTDMLTRLSDLLKYFNSRSCQLVLGAGALQVVGLKTITTKNLALSSRCLQLIVHYIPLIRAHFEARMQPKQYSMLRHFDHITKDYNDHIAEISAKLVTIMDGLFEKLLSKYEVKAPVPSTCFRTICKQMAKMHEAIYDLLPEEQTQTLFLKINSSYIFHLRRQLCHLNVINDGGPQYGLVAADIAFYTENVQALKGLKDLDLSLAEIWEQKR

Target ORF information:

RefSeq Version	XM_002939319.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis VPS54 GARP complex subunit (vps54), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002939319.5

ORF Insert Sequence:

ATGTATGCAA TGGCCTCCAG CCACAGCTCT TCACTAGTGC CTCACTCGAA CAGCAACAAA 
GCTTTTTACA AAAAGGGTCT TCAGCCTGCT CAGCAAGTGG GAATGGTGCG ATCACTGCCT 
GATGTCTGCC CCAAGGAGCC TACAGGTGAT ACTAAGAGTT TGCGTGATGA ACCATCTGTA 
GTTGCAGATC AGCACAAATG GACAATTTAT AATTCTAAAG TAAACCTTCC GGCAGCATTA 
AATGACCCCC GCCTTGCAAA ACGGGAATCA GACTTTTTCA CAAAAACATG GGGGCAAGAC 
TTTGTGGAAA GTGAAGTCCT ACCATCACCC TACCTCCCAG CAATTACTAA GGAACAGTTT 
CTATCATATT CGCAGCAAAC CATCAGTAGA GAAAAAAATC ATGAAAAATG TAAGAGTGTT 
TGCCCACTAA AGGTGGACTT TGACAGACCA GCCTATTCTC ATGTTCTCAA ATCGAGAGCA 
GATCTGGAAC AAGTACCTAA GATCTTTATG AAGCCAGACT TTGCCTTGGA GGACTCCTTA 
ACATTTAATG CTGTTTTGCC ATGGTCTCAT TTTAGCAGTG CCGGAGGGAA AGGAAGCAGA 
GATGCAGCAT CCTCGAAATT ACTTCAAGAA AAGCTAAGCC ACTATTTGGA TATTGTTGAG 
GTTAACATCG CTCACCAAAT CTCCTTGCGC TCTGAAGCAT TTTTCCATGC TATGACCTCT 
CAACATGAGC TGCAGGACTA CCTCAGGAAG ACTTCCCAGG CTGTTAAAAT GCTGCGAGAC 
AAGATCTCTC AGATAGACAG AGTCATGTGT GAGGGATCTC TGCGGATCTT ACGGCAAGCA 
CTGACCAGGA ACAACTGCAT TAAAATATAC AATAAGCTCA AGTTAATGGC AACAGTGCAT 
CAAACTCAGC CTACTGTACA GCTTCTATTA TCAACCTCTG AGTATGTAGG AGCTTTGGAC 
TTAATAGCAA CGACACAAGA GGTCTTGCAG CAGGAGTTAC AGGGTATTCA CAGTTTTCGA 
CATCTCAGCT CACAACTTTG CGAAATGGAA AAACTCATTG ATAAAATGAT CATCTCTGAA 
TTTACTTCGT ATGCTCGCAG CGATTTAAAC AGACCTTTAG AAGAAGACAG TCATGTAGTG 
GAAGAGGAAA GACTGGTCTC TCTCGTATTT GGGATGTTAA AGCAGCGAAA ACTGGATTTC 
TTTGAGATTT ACTCAGACGA AATGATGGCA ACAGCAAAAA ATATCATCAA GCAGTGTGTG 
GTTAACACTG TGTCTCAGAT TGAGGAAATA GACTCAGAAG TTGTTACAAA GCTTACTGAT 
CAGATTCGGA TGATGAATTT TTCTCAGTGG TTTGGCTTGC TTCAGAATAT TTTTTCTAAG 
TTTACAATTT TTATTGAGAG AGTTAAGGCA ACGCTAAGCG TCATCCGTAA TGTAGTGCTC 
ATGGTTTTAG ACAAAAATCA GCGGGTGAGG GAAATGGAAA TGTTGGCAGC TCAACAGAAT 
CTCAGAAGAG AAAGCAATGT GGACAATGAA TTGGCGTACT TGACTCATGA AGGCTTATTT 
ATAAGTGATG CTTTCGGTGA AGGTGAGCTT CCCAATGCTG CTACCAGCAC TAACTTTCAA 
AGGAACACAT CACCCAGCAG TAGCGAGCCC TCTGGCAGCG ATACAGTATC TGATCCTGAG 
TGTACCACAG ACTCTTCTTC CAGCAAAGAA CACACACTGC CTTCTGTTAC CCAAGGGGGA 
ATAGAAATAA TTCTAACTGA AGAAATGAAG TTAACTGACC TGGAACTTGG TAAAATTGCC 
AACAGTATAC AGGAGTTACT GTATGATGCC TCTGACATCT GCCATGACCG CTGCGTCAAG 
TTCCTGCTAG CGAGGGCCAA GGAAGGGTTT CTTGAGAAGC TAAACTCTGC TGAATTTGTG 
TCCCTCTCAC GGGCAGTGGA AGCATTCGTT TTGGGTACTG AAAAGATCTG TGGCCGAAGG 
AGCATGTCAC TGAGAGGGGC ACTGCAAAGC CAAGCCAACA AATTTGTAAA TCGGTTCCAT 
GATGAAAGAA AAACCAAACT GAGCCTCCTG CTTGATAATG AACGCTGGAA ACAAGCTGAT 
GTCCCTGCTG AATTTCAGGA CTTAGTAGAT TCTATAAAGG ACGGTAAAAT AGTGCTTCCT 
GAGCGAAAAT CTGGAGGTAC AGAAGAAAGG AAACCCACAG ATTTTCTTAC AGTGGACGGA 
CAGAAGTATG CAGTTGTTGG CACAGTGCTA CTTTTGATCA GAATCATTCT CGAGTACTGC 
AGCTGCGTAG ATGATATCCC CTCTGTAACT ACAGACATGT TGACTCGGCT GTCTGATCTT 
TTAAAGTATT TTAATTCACG GAGTTGCCAA TTGGTTCTGG GAGCAGGAGC ATTGCAAGTG 
GTTGGCCTGA AGACAATAAC CACCAAGAAC TTAGCCTTGT CTTCGCGGTG CTTGCAGCTT 
ATAGTACACT ACATTCCCCT CATAAGGGCC CACTTTGAAG CTCGGATGCA GCCTAAGCAG 
TATAGCATGC TCCGGCATTT TGACCATATA ACCAAGGACT ACAATGACCA CATTGCAGAG 
ATTTCTGCCA AGCTTGTCAC AATAATGGAT GGCCTGTTTG AAAAGCTACT TTCCAAGTAT 
GAGGTAAAAG CCCCAGTTCC TTCTACGTGT TTCAGAACCA TCTGCAAGCA AATGGCCAAG 
ATGCACGAGG CCATTTATGA CCTCCTTCCT GAAGAGCAAA CGCAGACTCT GTTTCTTAAA 
ATAAATTCAA GCTACATATT CCATTTGAGG CGACAATTGT GTCATTTAAA TGTCATTAAT 
GATGGAGGAC CACAATACGG ATTGGTGGCA GCTGACATTG CCTTTTACAC TGAGAACGTA 
CAAGCCTTGA AAGGCCTGAA AGATCTGGAT CTTAGCTTGG CTGAAATTTG GGAGCAGAAA 
AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vps54 ( XM_018094167.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949656.1 Xenopus tropicalis VPS54 GARP complex subunit (vps54), transcript variant X3, mRNA.

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CloneID	OXa44599
Clone ID Related Accession (Same CDS sequence)	XM_018094167.1
Accession Version	XM_018094167.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2865bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	vacuolar protein sorting-associated protein 54 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGCAA TGGCCTCCAG CCACAGCTCT TCACTAGTGC CTCACTCGAA CAGCAACAAA 
GCTTTTTACA AAAAGGGTCT TCAGCCTGCT CAGCAAGTGG GAATGGTGCG ATCACTGCCT 
GATGTCTGCC CCAAGGAGCC TACAGGTGAT ACTAAGAGTT TGCGTGATGA ACCATCTGTA 
GTTGCAGATC AGCACAAATG GACAATTTAT AATTCTAAAG TAAACCTTCC GGCAGCATTA 
AATGACCCCC GCCTTGCAAA ACGGGAATCA GACTTTTTCA CAAAAACATG GGGGCAAGAC 
TTTGTGGAAA GTGAAGTCCT ACCATCACCC TACCTCCCAG CAATTACTAA GGAACAGTTT 
CTATCATATT CGCAGCAAAC CATCAGTAGA GAAAAAAATC ATGAAAAATG TAAGAGTGTT 
TGCCCACTAA AGGTGGACTT TGACAGACCA GCCTATTCTC ATGTTCTCAA ATCGAGAGCA 
GATCTGGAAC AAGTACCTAA GATCTTTATG AAGCCAGACT TTGCCTTGGA GGACTCCTTA 
ACATTTAATG CTGTTTTGCC ATGGTCTCAT TTTAGCAGTG CCGGAGGGAA AGGAAGCAGA 
GATGCAGCAT CCTCGAAATT ACTTCAAGAA AAGCTAAGCC ACTATTTGGA TATTGTTGAG 
GTTAACATCG CTCACCAAAT CTCCTTGCGC TCTGAAGCAT TTTTCCATGC TATGACCTCT 
CAACATGAGC TGCAGGACTA CCTCAGGAAG ACTTCCCAGG CTGTTAAAAT GCTGCGAGAC 
AAGATCTCTC AGATAGACAG AGTCATGTGT GAGGGATCTC TGCGGATCTT ACGGCAAGCA 
CTGACCAGGA ACAACTGCAT TAAAATATAC AATAAGCTCA AGTTAATGGC AACAGTGCAT 
CAAACTCAGC CTACTGTACA GCTTCTATTA TCAACCTCTG AGTATGTAGG AGCTTTGGAC 
TTAATAGCAA CGACACAAGA GGTCTTGCAG CAGGAGTTAC AGGGTATTCA CAGTTTTCGA 
CATCTCAGCT CACAACTTTG CGAAATGGAA AAACTCATTG ATAAAATGAT CATCTCTGAA 
TTTACTTCGT ATGCTCGCAG CGATTTAAAC AGACCTTTAG AAGAAGACAG TCATGTAGTG 
GAAGAGGAAA GACTGGTCTC TCTCGTATTT GGGATGTTAA AGCAGCGAAA ACTGGATTTC 
TTTGAGATTT ACTCAGACGA AATGATGGCA ACAGCAAAAA ATATCATCAA GCAGTGTGTG 
GTTAACACTG TGTCTCAGAT TGAGGAAATA GACTCAGAAG TTGTTACAAA GCTTACTGAT 
CAGATTCGGA TGATGAATTT TTCTCAGTGG TTTGGCTTGC TTCAGAATAT TTTTTCTAAG 
TTTACAATTT TTATTGAGAG AGTTAAGGCA ACGCTAAGCG TCATCCGTAA TGTAGTGCTC 
ATGGTTTTAG ACAAAAATCA GCGGGTGAGG GAAATGGAAA TGTTGGCAGC TCAACAGAAT 
CTCAGAAGAG AAAGCAATGT GGACAATGAA TTGGCGTACT TGACTCATGA AGGCTTATTT 
ATAAGTGATG CTTTCGGTGA AGGTGAGCTT CCCAATGCTG CTACCAGCAC TAACTTTCAA 
AGGAACACAT CACCCAGCAG TAGCGAGCCC TCTGGCAGCG ATACAGTATC TGATCCTGAG 
TGTACCACAG ACTCTTCTTC CAGCAAAGAA CACACACTGC CTTCTGTTAC CCAAGGGGGA 
ATAGAAATAA TTCTAACTGA AGAAATGAAG TTAACTGACC TGGAACTTGG TAAAATTGCC 
AACAGTATAC AGGAGTTACT GTATGATGCC TCTGACATCT GCCATGACCG CTGCGTCAAG 
TTCCTGCTAG CGAGGGCCAA GGAAGGGTTT CTTGAGAAGC TAAACTCTGC TGAATTTGTG 
TCCCTCTCAC GGGCAGTGGA AGCATTCGTT TTGGGTACTG AAAAGATCTG TGGCCGAAGG 
AGCATGTCAC TGAGAGGGGC ACTGCAAAGC CAAGCCAACA AATTTGTAAA TCGGTTCCAT 
GATGAAAGAA AAACCAAACT GAGCCTCCTG CTTGATAATG AACGCTGGAA ACAAGCTGAT 
GTCCCTGCTG AATTTCAGGA CTTAGTAGAT TCTATAAAGG ACGGTAAAAT AGTGCTTCCT 
GAGCGAAAAT CTGGAGGTAC AGAAGAAAGG AAACCCACAG ATTTTCTTAC AGTGGACGGA 
CAGAAGTATG CAGTTGTTGG CACAGTGCTA CTTTTGATCA GAATCATTCT CGAGTACTGC 
AGCTGCGTAG ATGATATCCC CTCTGTAACT ACAGACATGT TGACTCGGCT GTCTGATCTT 
TTAAAGTATT TTAATTCACG GAGTTGCCAA TTGGTTCTGG GAGCAGGAGC ATTGCAAGTG 
GTTGGCCTGA AGACAATAAC CACCAAGAAC TTAGCCTTGT CTTCGCGGTG CTTGCAGCTT 
ATAGTACACT ACATTCCCCT CATAAGGGCC CACTTTGAAG CTCGGATGCA GCCTAAGCAG 
TATAGCATGC TCCGGCATTT TGACCATATA ACCAAGTATG AGGTAAAAGC CCCAGTTCCT 
TCTACGTGTT TCAGAACCAT CTGCAAGCAA ATGGCCAAGA TGCACGAGGC CATTTATGAC 
CTCCTTCCTG AAGAGCAAAC GCAGACTCTG TTTCTTAAAA TAAATTCAAG CTACATATTC 
CATTTGAGGC GACAATTGTG TCATTTAAAT GTCATTAATG ATGGAGGACC ACAATACGGA 
TTGGTGGCAG CTGACATTGC CTTTTACACT GAGAACGTAC AAGCCTTGAA AGGCCTGAAA 
GATCTGGATC TTAGCTTGGC TGAAATTTGG GAGCAGAAAA GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949656.1
CDS	348..3212
Translation	MYAMASSHSSSLVPHSNSNKAFYKKGLQPAQQVGMVRSLPDVCPKEPTGDTKSLRDEPSVVADQHKWTIYNSKVNLPAALNDPRLAKRESDFFTKTWGQDFVESEVLPSPYLPAITKEQFLSYSQQTISREKNHEKCKSVCPLKVDFDRPAYSHVLKSRADLEQVPKIFMKPDFALEDSLTFNAVLPWSHFSSAGGKGSRDAASSKLLQEKLSHYLDIVEVNIAHQISLRSEAFFHAMTSQHELQDYLRKTSQAVKMLRDKISQIDRVMCEGSLRILRQALTRNNCIKIYNKLKLMATVHQTQPTVQLLLSTSEYVGALDLIATTQEVLQQELQGIHSFRHLSSQLCEMEKLIDKMIISEFTSYARSDLNRPLEEDSHVVEEERLVSLVFGMLKQRKLDFFEIYSDEMMATAKNIIKQCVVNTVSQIEEIDSEVVTKLTDQIRMMNFSQWFGLLQNIFSKFTIFIERVKATLSVIRNVVLMVLDKNQRVREMEMLAAQQNLRRESNVDNELAYLTHEGLFISDAFGEGELPNAATSTNFQRNTSPSSSEPSGSDTVSDPECTTDSSSSKEHTLPSVTQGGIEIILTEEMKLTDLELGKIANSIQELLYDASDICHDRCVKFLLARAKEGFLEKLNSAEFVSLSRAVEAFVLGTEKICGRRSMSLRGALQSQANKFVNRFHDERKTKLSLLLDNERWKQADVPAEFQDLVDSIKDGKIVLPERKSGGTEERKPTDFLTVDGQKYAVVGTVLLLIRIILEYCSCVDDIPSVTTDMLTRLSDLLKYFNSRSCQLVLGAGALQVVGLKTITTKNLALSSRCLQLIVHYIPLIRAHFEARMQPKQYSMLRHFDHITKYEVKAPVPSTCFRTICKQMAKMHEAIYDLLPEEQTQTLFLKINSSYIFHLRRQLCHLNVINDGGPQYGLVAADIAFYTENVQALKGLKDLDLSLAEIWEQKR

Target ORF information:

RefSeq Version	XM_018094167.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis VPS54 GARP complex subunit (vps54), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094167.1

ORF Insert Sequence:

ATGTATGCAA TGGCCTCCAG CCACAGCTCT TCACTAGTGC CTCACTCGAA CAGCAACAAA 
GCTTTTTACA AAAAGGGTCT TCAGCCTGCT CAGCAAGTGG GAATGGTGCG ATCACTGCCT 
GATGTCTGCC CCAAGGAGCC TACAGGTGAT ACTAAGAGTT TGCGTGATGA ACCATCTGTA 
GTTGCAGATC AGCACAAATG GACAATTTAT AATTCTAAAG TAAACCTTCC GGCAGCATTA 
AATGACCCCC GCCTTGCAAA ACGGGAATCA GACTTTTTCA CAAAAACATG GGGGCAAGAC 
TTTGTGGAAA GTGAAGTCCT ACCATCACCC TACCTCCCAG CAATTACTAA GGAACAGTTT 
CTATCATATT CGCAGCAAAC CATCAGTAGA GAAAAAAATC ATGAAAAATG TAAGAGTGTT 
TGCCCACTAA AGGTGGACTT TGACAGACCA GCCTATTCTC ATGTTCTCAA ATCGAGAGCA 
GATCTGGAAC AAGTACCTAA GATCTTTATG AAGCCAGACT TTGCCTTGGA GGACTCCTTA 
ACATTTAATG CTGTTTTGCC ATGGTCTCAT TTTAGCAGTG CCGGAGGGAA AGGAAGCAGA 
GATGCAGCAT CCTCGAAATT ACTTCAAGAA AAGCTAAGCC ACTATTTGGA TATTGTTGAG 
GTTAACATCG CTCACCAAAT CTCCTTGCGC TCTGAAGCAT TTTTCCATGC TATGACCTCT 
CAACATGAGC TGCAGGACTA CCTCAGGAAG ACTTCCCAGG CTGTTAAAAT GCTGCGAGAC 
AAGATCTCTC AGATAGACAG AGTCATGTGT GAGGGATCTC TGCGGATCTT ACGGCAAGCA 
CTGACCAGGA ACAACTGCAT TAAAATATAC AATAAGCTCA AGTTAATGGC AACAGTGCAT 
CAAACTCAGC CTACTGTACA GCTTCTATTA TCAACCTCTG AGTATGTAGG AGCTTTGGAC 
TTAATAGCAA CGACACAAGA GGTCTTGCAG CAGGAGTTAC AGGGTATTCA CAGTTTTCGA 
CATCTCAGCT CACAACTTTG CGAAATGGAA AAACTCATTG ATAAAATGAT CATCTCTGAA 
TTTACTTCGT ATGCTCGCAG CGATTTAAAC AGACCTTTAG AAGAAGACAG TCATGTAGTG 
GAAGAGGAAA GACTGGTCTC TCTCGTATTT GGGATGTTAA AGCAGCGAAA ACTGGATTTC 
TTTGAGATTT ACTCAGACGA AATGATGGCA ACAGCAAAAA ATATCATCAA GCAGTGTGTG 
GTTAACACTG TGTCTCAGAT TGAGGAAATA GACTCAGAAG TTGTTACAAA GCTTACTGAT 
CAGATTCGGA TGATGAATTT TTCTCAGTGG TTTGGCTTGC TTCAGAATAT TTTTTCTAAG 
TTTACAATTT TTATTGAGAG AGTTAAGGCA ACGCTAAGCG TCATCCGTAA TGTAGTGCTC 
ATGGTTTTAG ACAAAAATCA GCGGGTGAGG GAAATGGAAA TGTTGGCAGC TCAACAGAAT 
CTCAGAAGAG AAAGCAATGT GGACAATGAA TTGGCGTACT TGACTCATGA AGGCTTATTT 
ATAAGTGATG CTTTCGGTGA AGGTGAGCTT CCCAATGCTG CTACCAGCAC TAACTTTCAA 
AGGAACACAT CACCCAGCAG TAGCGAGCCC TCTGGCAGCG ATACAGTATC TGATCCTGAG 
TGTACCACAG ACTCTTCTTC CAGCAAAGAA CACACACTGC CTTCTGTTAC CCAAGGGGGA 
ATAGAAATAA TTCTAACTGA AGAAATGAAG TTAACTGACC TGGAACTTGG TAAAATTGCC 
AACAGTATAC AGGAGTTACT GTATGATGCC TCTGACATCT GCCATGACCG CTGCGTCAAG 
TTCCTGCTAG CGAGGGCCAA GGAAGGGTTT CTTGAGAAGC TAAACTCTGC TGAATTTGTG 
TCCCTCTCAC GGGCAGTGGA AGCATTCGTT TTGGGTACTG AAAAGATCTG TGGCCGAAGG 
AGCATGTCAC TGAGAGGGGC ACTGCAAAGC CAAGCCAACA AATTTGTAAA TCGGTTCCAT 
GATGAAAGAA AAACCAAACT GAGCCTCCTG CTTGATAATG AACGCTGGAA ACAAGCTGAT 
GTCCCTGCTG AATTTCAGGA CTTAGTAGAT TCTATAAAGG ACGGTAAAAT AGTGCTTCCT 
GAGCGAAAAT CTGGAGGTAC AGAAGAAAGG AAACCCACAG ATTTTCTTAC AGTGGACGGA 
CAGAAGTATG CAGTTGTTGG CACAGTGCTA CTTTTGATCA GAATCATTCT CGAGTACTGC 
AGCTGCGTAG ATGATATCCC CTCTGTAACT ACAGACATGT TGACTCGGCT GTCTGATCTT 
TTAAAGTATT TTAATTCACG GAGTTGCCAA TTGGTTCTGG GAGCAGGAGC ATTGCAAGTG 
GTTGGCCTGA AGACAATAAC CACCAAGAAC TTAGCCTTGT CTTCGCGGTG CTTGCAGCTT 
ATAGTACACT ACATTCCCCT CATAAGGGCC CACTTTGAAG CTCGGATGCA GCCTAAGCAG 
TATAGCATGC TCCGGCATTT TGACCATATA ACCAAGTATG AGGTAAAAGC CCCAGTTCCT 
TCTACGTGTT TCAGAACCAT CTGCAAGCAA ATGGCCAAGA TGCACGAGGC CATTTATGAC 
CTCCTTCCTG AAGAGCAAAC GCAGACTCTG TTTCTTAAAA TAAATTCAAG CTACATATTC 
CATTTGAGGC GACAATTGTG TCATTTAAAT GTCATTAATG ATGGAGGACC ACAATACGGA 
TTGGTGGCAG CTGACATTGC CTTTTACACT GAGAACGTAC AAGCCTTGAA AGGCCTGAAA 
GATCTGGATC TTAGCTTGGC TGAAATTTGG GAGCAGAAAA GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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vps54 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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