cep295 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	cep295
Entrez Gene ID	100379879
Full Name	centrosomal protein 295
Synonyms	kiaa1731
General protein information	Preferred Names centrosomal protein 295 Names centrosomal protein of 295 kDa centrosomal protein 295kDa
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001199923.1	NP_001186852.1	centrosomal protein of 295 kDa
XM_031895775.1	XP_031751635.1	centrosomal protein of 295 kDa isoform X1
XM_031895778.1	XP_031751638.1	centrosomal protein of 295 kDa isoform X2
XM_031895779.1	XP_031751639.1	centrosomal protein of 295 kDa isoform X3
XM_031895776.1	XP_031751636.1	centrosomal protein of 295 kDa isoform X1
XM_031895777.1	XP_031751637.1	centrosomal protein of 295 kDa isoform X1
XM_012956604.2	XP_012812058.1	centrosomal protein of 295 kDa isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	KIAA1731	XP_003432993.1
Gallus gallus (chicken)	KIAA1731	XP_004938927.1
Xenopus tropicalis (tropical clawed frog)	kiaa1731	NP_001186852.1

Gene Ontology

The following cep295 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cep295 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa51205	XM_031895777.1 Latest version!	Xenopus tropicalis centrosomal protein 295 (cep295), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa51205	XM_031895775.1 Latest version!	Xenopus tropicalis centrosomal protein 295 (cep295), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa51205	XM_031895776.1 Latest version!	Xenopus tropicalis centrosomal protein 295 (cep295), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa51206	XM_031895778.1 Latest version!	Xenopus tropicalis centrosomal protein 295 (cep295), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa51207	XM_031895779.1 Latest version!	Xenopus tropicalis centrosomal protein 295 (cep295), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa24275	NM_001199923.1 Latest version!	Xenopus tropicalis centrosomal protein 295 (cep295), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa32965	XM_012956604.2 Latest version!	Xenopus tropicalis centrosomal protein 295kDa (cep295), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cep295 ( XM_031895775.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751635.1 Xenopus tropicalis centrosomal protein 295 (cep295), transcript variant X1, mRNA.

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CloneID	OXa51205
Clone ID Related Accession (Same CDS sequence)	XM_031895775.1 , XM_031895776.1 , XM_031895777.1
Accession Version	XM_031895775.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5349bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	centrosomal protein of 295 kDa isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACGCA AAGTTGCAAA TACCGGCGTC ATAAGACTTA GCCCCAATGA AGAAGCCTTG 
CTGCTAAAGC AGGAACGTGA AAGAAGGCGC AAACTCCGCT TACAACAGGT TAGAGAACAG 
GAGAAGTTCA TTGCCCTGCA AATCCGTCAC AATGTTAAAG AAAGGAGGGA ACAGGAGCTT 
CAGAATTTGG CAGAGGAGCT GCGCTTGGAG TGGCAGGCAT CGCAGGCTGA GAAGCTCCGA 
GCCCTGGAGA AGCTTTATGT GTCCAGTCTA AGTGCGATTG GAGAGGGGCA CAGGCAAGCC 
AAGGAAAATG AACCGGACCT TCAAGCCGTT GAGAGGCAAG CGGCAGCCAA TAGACAGAAA 
GCAGAGAAGC GGCACAGAGA AGCTCTCAGG GAATTAAAGC AGCACAAAGA AAAACAATTG 
AGAGAGCAAA CCTGGCTTTT GAAAGCCCGC AGGAAAGCGC TTATTACTGA GAAGGAGCGT 
GCTGCAAAGA TAGCAAGCCT TCCTCCCCCA CCCCCAGACC CTCTAGAGAA TATTGAGGTT 
TTGCGAAGGT TCCCAACCGT GAAGCTGTGC AATGTTGATG GCTTCTCTGT ATCTCATTAT 
CATCTGCCTG AACCATACGT AGACCGAGAG ACGGATACGG AACAGCCAGA TGCTCGGGTT 
GCCGCTGAAG AGGAAGCAAA GCGTTTGGAT GCCTTACAGA AAGAGGAAGA GAGAGAAAGG 
AGGGAAAGGA TGGAGAAGGC TCACTTACGG GGATCCCATG CCTTGAAAAA GGTTCTTTTA 
ACCCAAGACC GGGACAAACT GCTGAAAGAA CTGGAACAGA TGCAGCAAGA GGATTTGGCT 
CAGAGGAGGC AGGTTGTCGC CCAGATGCCT CAGCAGCTAT TTGAACCGGC CTATAGGCGA 
GAAGAGATAC AGGAGGAGTG GCAGAGGGAA CTGGAGGTTG CTTTTGAAGA CATGTACACT 
GGAGAAAGAA GGGTTAGAGG GGACATGGTT TTGCACCTCA AGCCACAGCC CTTACCAGTT 
CCATCTGCTG AAAGTTTTGA TGATGACCTG GACCTGTCTG TGGATCCAGA GCCCGTGTGT 
GAAACCCAGC CACAAACTGA TGCTGTTGAG GAACCAAGTC ACATTGAGAA AACGGATGAC 
AAAAGCCGCG AGCCACAATC GAGAATTGTT TTTAAGAGAC TGTTGAATAA AATACGAAGC 
CAGCAGGATC AGTGGAGCGC CAAAAGTGAT GTGGAGACGC AGAGCGACAC TGTGGAATCG 
GGATCTCTGC CTAATGAAAT ACATACAATC CATGCAGAAC TCCATGAGGC AAGGAAGCCT 
GCGCCTGAGG GCATTCAGAA CATGAGTGAT AGTACCATTC TAGCTGGTAA TGCAGTTCCT 
CGTCATGCAA CGGAGCCAGG AACACCAACA AAACAGGCCT CTGATTGGAA CAAGCAGGAT 
TTAGAGAGGC AGAAACAGGA GCAGCTGGAT TTGCTGAACA GACTAGAAGA GGAGAAAAAA 
CGCTTAGAAG CCGAATACTT GAGGGTTCAA CTCCAGATGC AAAAATCAAA GCAAGAAGGA 
ATGGAACCTC CAGAGGAGGA GGAGGAGGAA GAGGCAGCTG TAATTCCTCC TGCAGTGACT 
GAACCCAAAA CCATGGAAGA GCCCAAACCT GCGCAGGAAC CTCCAGGTTT AAGCCTAAAT 
GTTTCCACCG AGAGCCCGCA CATTCAGATA ATTCGACAGT ACCAGCAACG TCTAATTGAG 
CAAAACAGAC AACACAAGCA GTCGGTAGAA GAGGCGAGAC AACGTTTGCA AGAATACCAG 
CAATTGCTGA AAAAACGCTA TTCGCATTTG TCTGCCTCGA GGCTGCAGCC TGCAGCCAAG 
GAACCCGTTA GTTTCGCTAT AAAGCCCGGT CTCGCACAGA AACCTGCACA GCACTTGGAA 
GACATTGGCG GTGCTTCAGA CTCCCATGTT AGAGTAACCT CTTACCCTGG GGAGAGTCTA 
GCAGGGCACC TTGTATCTCT GGGTAGCTCT TTAATATCTG CAGTGCCGGT TTCTGGACAA 
AACTTATTTG CACCTTCACC AGTAAGCAGA ACAGCAGAGT TTGGACAGTC GGGAAGCAGT 
CCCCTCCCTC ATTCTACAGA GCAAGATAAC TTTATTCCAT ACTCTTCTGG GACTATTCCA 
ACACAACAAC AATATGGAGG GGCAGAAAGC ATGACCGGAA AAGACATTGC TGCGAACGGC 
ATTAAAAGTC CTAAAAGAGC AACACCGTTG GGAAATTTTA TTTTGGAAGT GGCAGCTCCC 
AGGCTAGAAC AATACACTTC TTCGCCCGAA GAAGACTCCA TCAGTCCTCC ATATTCTATG 
TACGATCTGG AAAAGCAGTC CTCCACATCA AGCAAAGGTG ATTATGATAC GGCCTCTGAT 
AGTTTCTACG CCCTTCCTTC TTCCTTGTCT TTGGGTCTGC CACAGAATAT AGAGAATATA 
GAGCAAGAAC CTCATGTTCA AAAAGTGGAT AGTGTTCCAT TCGGAGAGTT CTCTAATGTG 
CACGAGTTCC GCAAGGGGCT GATCTCTTCA ACGGCCAAGA TGCAAGCTCA GCAGACTCAG 
CTTAGGGATA TGCAGCTGCA GCTGGAGATG CAGAGGGAGT CCCTCTTGTC TAAACAGAAA 
TCCCAAGAAG AAGGTCTGTT ACAGAAACAG AAAGAGCTGG AAGATCAAAT GCGCAAGCAC 
CAAGAGTCGT TGGAACATTT CCTGGCCAAC AAGGAGTCTG CACAAGGGGC GTTACCTCCT 
GATCTCTGTC AGATTTCAAA TAACGAACGT CTTCGCCTTA TGTCTGCGCT GCTGAAAGCA 
CTGGGTGACG GTAAACAAGA GAACCTCAGT CTGGGAAATG GTAGTAGCCT TAGAGCTGCT 
GGGAGAGAAC TGAAAGGGAG ACCGTCTAAA CCTCCGGTTA CAAAAACCAA ATTGGGTCCA 
TCGTTGGAAC AACATGAGCT CAGTGCTATT CTGGAAGTGG AAACGCCAAG TGGCAGGCCT 
TCTAGTACTG GGCCGCCGGA ACACAAGAAC AGCAGCTCAG GCCGACAGGA TGGTGCTGAG 
GGTGGGAACT CGTCTGTGCC CCCTGCTTGC ATGCAAGAAA ACAGCAGACT GAGGGAAGCC 
GGTAACTATT CTCTCGGAGA TCCGGATCTT TCCAGAACAT CGACGAGCAG CAATGAAAGC 
AATAGGAGCC AGACTAAGTT ATCTTGGAGA CAGATGTTGT CTCTAGCAGC TGCTTCATCC 
CATAACTTGA CTCTCCCAGA TCAATCCTGG AGCTCTTCAG TGCACTCAGG CCTTTCTCCT 
CATCCTGCTG GTACCAAAAC AGCATTCCAC TTTCAGCAAC CTGCCCAGGA GAGATCCGAT 
TGGCTGGGGG CAGCTGAGCT GGGAAAAACA GGCATTGGAT ACAGTGCTCA AACGCAGGCC 
CGGGAAGCTT TCAGCGATCA CTTGTCAACA ACCACAATCT CTACGGGAAG TTTTCTGACC 
AGCGAGAAGG CCGATTCAAG CATTAGCAAC TTAGAGACTA TTTCAGACCT TCACAAATGC 
AACTTCTCCA TTATCAATGG CGCGGAACAC GGCAACTGGA GTACCTCAGT CAACGGGGTT 
CCACAGCCGA CTAGAAGGGA TGAGTTCCCG AGCCCGGAAT ATTTGTCGAC TGCACCGGAT 
CAGCGCAGCC ACATTCAGCA GATCATTGAT AAATACACCA GGGATCTCAG TGCTTCCCTA 
GAGAGAAATC TAAGCTTTCA TACTCCTACT GCTGCTCTAG ATGTATCTGG GAATGACAAT 
CACCTGCCCT CGGGTTTCTA TGCCCTGGAA CCGAAACCTG ACTTCAATGT CTCTACAACT 
TCCCATTCAC AATTAAACAA CACCGGCAGC TCTCAAGAGT CAAAGAACAC AAGCCAGAGC 
TCGTTTTACC CAAGCAGCAA AGAGTGGTCT TCAGAACAAT CTCATAGCTT CCAGTATCCA 
GACAGCAGCC ATAGTCCGAT GTCTTCCCCT GGCATAACTA GTGCGCAGCA CGTGGATGTG 
GAAATCACCC CTCATGTTCG TGGGGATCAA ACTCTAGGTT CCTCTGGATC GTTCCACCCT 
CTGCACCCAG AATGCACCCT TAATGAGCCA CCGCTGAGCA GCCCAGGTCT CTGCGGATGC 
AGCAGTGCAG TTGATGATCT GGGAAATGGA AGCATGAAGG CATCTAAACA ACATGCCTTA 
CAATCATCCA GTGTTGACTC TGGCAATTGG AACACAAACA GCAGCCCTGG TGCCTTTCAG 
CCTTCCGTGG AACCTCTCCG GGCCGATAGC CACACCTTGC TGGAGGATCT GGGCTCTTTT 
CATGAGCTGA TGGCCACAGA AACGACGGCT AATGAATCTG AGCTGTCTGA GCACCCACTC 
ACAGCTGTCG GCAAAACCAA ACATGGCGAC TCTCAGCAGC TTCATGAATT GCCTGTATTG 
TACGAAGCAC TCGAACCGGC AGAAATAGAG GCATCTGTAG GGGACTATCC AGGCCTGGGC 
TCCGTGCCAC AGCATTCCAA CGGTGATGAA GGGGCAGAAG TGGAGTCTTC ATCTCTGACC 
TTGAAAAGCG ACACACTTAC TGAGGCAGAA TCCAGCGTAC ACCCAGATAG TTCATCTGCC 
ATCAGCTCTT CTGCATTCTC TTTGGGCAGC TTTTCTCTTC TCAGGGACAC ACACAATGCC 
CGAGGCATCA TGGAAGAACC TGAGTTATCG CTAGTGAGTT TCCCTGACAG CAGCTTTGCT 
GGTTCAGAGC ACGTTACCAC CTTGACTAGC ATGTCGGATC ACCACGTGGA CCCAAGCTTT 
GCAGAGGGTT GCTTTCATCC CCTACCAGCT GAAGTGGACC ACTCCATGTT TAACATGCCA 
GACTGGTCAG CAGCGGGACC AAGCTTTACT GACCAATCTA CACCACAGCA CTTTGCAGCA 
CTTGATTGGA CATCCCCTGT GAGCCTGCAG GAAGCCTTAT TAAAGAAGAA AAAACATTTT 
ATCGAAAGCT CTTCAAAACG AGTTGAACAA ATCAAGCAGA AAGCTGCGGC AGTCGGAAAC 
CCTGTATTTA ACTCCACGCC GAATGTTGGA GTCAGCGCGC AGGAGGAGGC GTCTGCCTGT 
GACGAAGCTT CAGCAAGTGA CAGCAGCACC CTGAAGAAGG TTGTAGAAGT TCGTGTGTGT 
ACCCCGGAGG AGCGCAAGCT GTCGGAGATA GAGATGCACC AGAGGACTAT AAGGTTGTAC 
AACCAGCTCG ACGAAGTTAA AACCAAAAAA GAGGAGAAGA TGAGAAAGGA GTCCTATGCC 
AGAAACAGAG AGAAGGCTAA CGAGTTTAAG AAGAAAACTC TGGAAAAGCT GAGATCCAGG 
AGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751635.1
CDS	71..5419
Translation	MKRKVANTGVIRLSPNEEALLLKQERERRRKLRLQQVREQEKFIALQIRHNVKERREQELQNLAEELRLEWQASQAEKLRALEKLYVSSLSAIGEGHRQAKENEPDLQAVERQAAANRQKAEKRHREALRELKQHKEKQLREQTWLLKARRKALITEKERAAKIASLPPPPPDPLENIEVLRRFPTVKLCNVDGFSVSHYHLPEPYVDRETDTEQPDARVAAEEEAKRLDALQKEEERERRERMEKAHLRGSHALKKVLLTQDRDKLLKELEQMQQEDLAQRRQVVAQMPQQLFEPAYRREEIQEEWQRELEVAFEDMYTGERRVRGDMVLHLKPQPLPVPSAESFDDDLDLSVDPEPVCETQPQTDAVEEPSHIEKTDDKSREPQSRIVFKRLLNKIRSQQDQWSAKSDVETQSDTVESGSLPNEIHTIHAELHEARKPAPEGIQNMSDSTILAGNAVPRHATEPGTPTKQASDWNKQDLERQKQEQLDLLNRLEEEKKRLEAEYLRVQLQMQKSKQEGMEPPEEEEEEEAAVIPPAVTEPKTMEEPKPAQEPPGLSLNVSTESPHIQIIRQYQQRLIEQNRQHKQSVEEARQRLQEYQQLLKKRYSHLSASRLQPAAKEPVSFAIKPGLAQKPAQHLEDIGGASDSHVRVTSYPGESLAGHLVSLGSSLISAVPVSGQNLFAPSPVSRTAEFGQSGSSPLPHSTEQDNFIPYSSGTIPTQQQYGGAESMTGKDIAANGIKSPKRATPLGNFILEVAAPRLEQYTSSPEEDSISPPYSMYDLEKQSSTSSKGDYDTASDSFYALPSSLSLGLPQNIENIEQEPHVQKVDSVPFGEFSNVHEFRKGLISSTAKMQAQQTQLRDMQLQLEMQRESLLSKQKSQEEGLLQKQKELEDQMRKHQESLEHFLANKESAQGALPPDLCQISNNERLRLMSALLKALGDGKQENLSLGNGSSLRAAGRELKGRPSKPPVTKTKLGPSLEQHELSAILEVETPSGRPSSTGPPEHKNSSSGRQDGAEGGNSSVPPACMQENSRLREAGNYSLGDPDLSRTSTSSNESNRSQTKLSWRQMLSLAAASSHNLTLPDQSWSSSVHSGLSPHPAGTKTAFHFQQPAQERSDWLGAAELGKTGIGYSAQTQAREAFSDHLSTTTISTGSFLTSEKADSSISNLETISDLHKCNFSIINGAEHGNWSTSVNGVPQPTRRDEFPSPEYLSTAPDQRSHIQQIIDKYTRDLSASLERNLSFHTPTAALDVSGNDNHLPSGFYALEPKPDFNVSTTSHSQLNNTGSSQESKNTSQSSFYPSSKEWSSEQSHSFQYPDSSHSPMSSPGITSAQHVDVEITPHVRGDQTLGSSGSFHPLHPECTLNEPPLSSPGLCGCSSAVDDLGNGSMKASKQHALQSSSVDSGNWNTNSSPGAFQPSVEPLRADSHTLLEDLGSFHELMATETTANESELSEHPLTAVGKTKHGDSQQLHELPVLYEALEPAEIEASVGDYPGLGSVPQHSNGDEGAEVESSSLTLKSDTLTEAESSVHPDSSSAISSSAFSLGSFSLLRDTHNARGIMEEPELSLVSFPDSSFAGSEHVTTLTSMSDHHVDPSFAEGCFHPLPAEVDHSMFNMPDWSAAGPSFTDQSTPQHFAALDWTSPVSLQEALLKKKKHFIESSSKRVEQIKQKAAAVGNPVFNSTPNVGVSAQEEASACDEASASDSSTLKKVVEVRVCTPEERKLSEIEMHQRTIRLYNQLDEVKTKKEEKMRKESYARNREKANEFKKKTLEKLRSRRK

Target ORF information:

RefSeq Version	XM_031895775.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis centrosomal protein 295 (cep295), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895775.1

ORF Insert Sequence:

ATGAAACGCA AAGTTGCAAA TACCGGCGTC ATAAGACTTA GCCCCAATGA AGAAGCCTTG 
CTGCTAAAGC AGGAACGTGA AAGAAGGCGC AAACTCCGCT TACAACAGGT TAGAGAACAG 
GAGAAGTTCA TTGCCCTGCA AATCCGTCAC AATGTTAAAG AAAGGAGGGA ACAGGAGCTT 
CAGAATTTGG CAGAGGAGCT GCGCTTGGAG TGGCAGGCAT CGCAGGCTGA GAAGCTCCGA 
GCCCTGGAGA AGCTTTATGT GTCCAGTCTA AGTGCGATTG GAGAGGGGCA CAGGCAAGCC 
AAGGAAAATG AACCGGACCT TCAAGCCGTT GAGAGGCAAG CGGCAGCCAA TAGACAGAAA 
GCAGAGAAGC GGCACAGAGA AGCTCTCAGG GAATTAAAGC AGCACAAAGA AAAACAATTG 
AGAGAGCAAA CCTGGCTTTT GAAAGCCCGC AGGAAAGCGC TTATTACTGA GAAGGAGCGT 
GCTGCAAAGA TAGCAAGCCT TCCTCCCCCA CCCCCAGACC CTCTAGAGAA TATTGAGGTT 
TTGCGAAGGT TCCCAACCGT GAAGCTGTGC AATGTTGATG GCTTCTCTGT ATCTCATTAT 
CATCTGCCTG AACCATACGT AGACCGAGAG ACGGATACGG AACAGCCAGA TGCTCGGGTT 
GCCGCTGAAG AGGAAGCAAA GCGTTTGGAT GCCTTACAGA AAGAGGAAGA GAGAGAAAGG 
AGGGAAAGGA TGGAGAAGGC TCACTTACGG GGATCCCATG CCTTGAAAAA GGTTCTTTTA 
ACCCAAGACC GGGACAAACT GCTGAAAGAA CTGGAACAGA TGCAGCAAGA GGATTTGGCT 
CAGAGGAGGC AGGTTGTCGC CCAGATGCCT CAGCAGCTAT TTGAACCGGC CTATAGGCGA 
GAAGAGATAC AGGAGGAGTG GCAGAGGGAA CTGGAGGTTG CTTTTGAAGA CATGTACACT 
GGAGAAAGAA GGGTTAGAGG GGACATGGTT TTGCACCTCA AGCCACAGCC CTTACCAGTT 
CCATCTGCTG AAAGTTTTGA TGATGACCTG GACCTGTCTG TGGATCCAGA GCCCGTGTGT 
GAAACCCAGC CACAAACTGA TGCTGTTGAG GAACCAAGTC ACATTGAGAA AACGGATGAC 
AAAAGCCGCG AGCCACAATC GAGAATTGTT TTTAAGAGAC TGTTGAATAA AATACGAAGC 
CAGCAGGATC AGTGGAGCGC CAAAAGTGAT GTGGAGACGC AGAGCGACAC TGTGGAATCG 
GGATCTCTGC CTAATGAAAT ACATACAATC CATGCAGAAC TCCATGAGGC AAGGAAGCCT 
GCGCCTGAGG GCATTCAGAA CATGAGTGAT AGTACCATTC TAGCTGGTAA TGCAGTTCCT 
CGTCATGCAA CGGAGCCAGG AACACCAACA AAACAGGCCT CTGATTGGAA CAAGCAGGAT 
TTAGAGAGGC AGAAACAGGA GCAGCTGGAT TTGCTGAACA GACTAGAAGA GGAGAAAAAA 
CGCTTAGAAG CCGAATACTT GAGGGTTCAA CTCCAGATGC AAAAATCAAA GCAAGAAGGA 
ATGGAACCTC CAGAGGAGGA GGAGGAGGAA GAGGCAGCTG TAATTCCTCC TGCAGTGACT 
GAACCCAAAA CCATGGAAGA GCCCAAACCT GCGCAGGAAC CTCCAGGTTT AAGCCTAAAT 
GTTTCCACCG AGAGCCCGCA CATTCAGATA ATTCGACAGT ACCAGCAACG TCTAATTGAG 
CAAAACAGAC AACACAAGCA GTCGGTAGAA GAGGCGAGAC AACGTTTGCA AGAATACCAG 
CAATTGCTGA AAAAACGCTA TTCGCATTTG TCTGCCTCGA GGCTGCAGCC TGCAGCCAAG 
GAACCCGTTA GTTTCGCTAT AAAGCCCGGT CTCGCACAGA AACCTGCACA GCACTTGGAA 
GACATTGGCG GTGCTTCAGA CTCCCATGTT AGAGTAACCT CTTACCCTGG GGAGAGTCTA 
GCAGGGCACC TTGTATCTCT GGGTAGCTCT TTAATATCTG CAGTGCCGGT TTCTGGACAA 
AACTTATTTG CACCTTCACC AGTAAGCAGA ACAGCAGAGT TTGGACAGTC GGGAAGCAGT 
CCCCTCCCTC ATTCTACAGA GCAAGATAAC TTTATTCCAT ACTCTTCTGG GACTATTCCA 
ACACAACAAC AATATGGAGG GGCAGAAAGC ATGACCGGAA AAGACATTGC TGCGAACGGC 
ATTAAAAGTC CTAAAAGAGC AACACCGTTG GGAAATTTTA TTTTGGAAGT GGCAGCTCCC 
AGGCTAGAAC AATACACTTC TTCGCCCGAA GAAGACTCCA TCAGTCCTCC ATATTCTATG 
TACGATCTGG AAAAGCAGTC CTCCACATCA AGCAAAGGTG ATTATGATAC GGCCTCTGAT 
AGTTTCTACG CCCTTCCTTC TTCCTTGTCT TTGGGTCTGC CACAGAATAT AGAGAATATA 
GAGCAAGAAC CTCATGTTCA AAAAGTGGAT AGTGTTCCAT TCGGAGAGTT CTCTAATGTG 
CACGAGTTCC GCAAGGGGCT GATCTCTTCA ACGGCCAAGA TGCAAGCTCA GCAGACTCAG 
CTTAGGGATA TGCAGCTGCA GCTGGAGATG CAGAGGGAGT CCCTCTTGTC TAAACAGAAA 
TCCCAAGAAG AAGGTCTGTT ACAGAAACAG AAAGAGCTGG AAGATCAAAT GCGCAAGCAC 
CAAGAGTCGT TGGAACATTT CCTGGCCAAC AAGGAGTCTG CACAAGGGGC GTTACCTCCT 
GATCTCTGTC AGATTTCAAA TAACGAACGT CTTCGCCTTA TGTCTGCGCT GCTGAAAGCA 
CTGGGTGACG GTAAACAAGA GAACCTCAGT CTGGGAAATG GTAGTAGCCT TAGAGCTGCT 
GGGAGAGAAC TGAAAGGGAG ACCGTCTAAA CCTCCGGTTA CAAAAACCAA ATTGGGTCCA 
TCGTTGGAAC AACATGAGCT CAGTGCTATT CTGGAAGTGG AAACGCCAAG TGGCAGGCCT 
TCTAGTACTG GGCCGCCGGA ACACAAGAAC AGCAGCTCAG GCCGACAGGA TGGTGCTGAG 
GGTGGGAACT CGTCTGTGCC CCCTGCTTGC ATGCAAGAAA ACAGCAGACT GAGGGAAGCC 
GGTAACTATT CTCTCGGAGA TCCGGATCTT TCCAGAACAT CGACGAGCAG CAATGAAAGC 
AATAGGAGCC AGACTAAGTT ATCTTGGAGA CAGATGTTGT CTCTAGCAGC TGCTTCATCC 
CATAACTTGA CTCTCCCAGA TCAATCCTGG AGCTCTTCAG TGCACTCAGG CCTTTCTCCT 
CATCCTGCTG GTACCAAAAC AGCATTCCAC TTTCAGCAAC CTGCCCAGGA GAGATCCGAT 
TGGCTGGGGG CAGCTGAGCT GGGAAAAACA GGCATTGGAT ACAGTGCTCA AACGCAGGCC 
CGGGAAGCTT TCAGCGATCA CTTGTCAACA ACCACAATCT CTACGGGAAG TTTTCTGACC 
AGCGAGAAGG CCGATTCAAG CATTAGCAAC TTAGAGACTA TTTCAGACCT TCACAAATGC 
AACTTCTCCA TTATCAATGG CGCGGAACAC GGCAACTGGA GTACCTCAGT CAACGGGGTT 
CCACAGCCGA CTAGAAGGGA TGAGTTCCCG AGCCCGGAAT ATTTGTCGAC TGCACCGGAT 
CAGCGCAGCC ACATTCAGCA GATCATTGAT AAATACACCA GGGATCTCAG TGCTTCCCTA 
GAGAGAAATC TAAGCTTTCA TACTCCTACT GCTGCTCTAG ATGTATCTGG GAATGACAAT 
CACCTGCCCT CGGGTTTCTA TGCCCTGGAA CCGAAACCTG ACTTCAATGT CTCTACAACT 
TCCCATTCAC AATTAAACAA CACCGGCAGC TCTCAAGAGT CAAAGAACAC AAGCCAGAGC 
TCGTTTTACC CAAGCAGCAA AGAGTGGTCT TCAGAACAAT CTCATAGCTT CCAGTATCCA 
GACAGCAGCC ATAGTCCGAT GTCTTCCCCT GGCATAACTA GTGCGCAGCA CGTGGATGTG 
GAAATCACCC CTCATGTTCG TGGGGATCAA ACTCTAGGTT CCTCTGGATC GTTCCACCCT 
CTGCACCCAG AATGCACCCT TAATGAGCCA CCGCTGAGCA GCCCAGGTCT CTGCGGATGC 
AGCAGTGCAG TTGATGATCT GGGAAATGGA AGCATGAAGG CATCTAAACA ACATGCCTTA 
CAATCATCCA GTGTTGACTC TGGCAATTGG AACACAAACA GCAGCCCTGG TGCCTTTCAG 
CCTTCCGTGG AACCTCTCCG GGCCGATAGC CACACCTTGC TGGAGGATCT GGGCTCTTTT 
CATGAGCTGA TGGCCACAGA AACGACGGCT AATGAATCTG AGCTGTCTGA GCACCCACTC 
ACAGCTGTCG GCAAAACCAA ACATGGCGAC TCTCAGCAGC TTCATGAATT GCCTGTATTG 
TACGAAGCAC TCGAACCGGC AGAAATAGAG GCATCTGTAG GGGACTATCC AGGCCTGGGC 
TCCGTGCCAC AGCATTCCAA CGGTGATGAA GGGGCAGAAG TGGAGTCTTC ATCTCTGACC 
TTGAAAAGCG ACACACTTAC TGAGGCAGAA TCCAGCGTAC ACCCAGATAG TTCATCTGCC 
ATCAGCTCTT CTGCATTCTC TTTGGGCAGC TTTTCTCTTC TCAGGGACAC ACACAATGCC 
CGAGGCATCA TGGAAGAACC TGAGTTATCG CTAGTGAGTT TCCCTGACAG CAGCTTTGCT 
GGTTCAGAGC ACGTTACCAC CTTGACTAGC ATGTCGGATC ACCACGTGGA CCCAAGCTTT 
GCAGAGGGTT GCTTTCATCC CCTACCAGCT GAAGTGGACC ACTCCATGTT TAACATGCCA 
GACTGGTCAG CAGCGGGACC AAGCTTTACT GACCAATCTA CACCACAGCA CTTTGCAGCA 
CTTGATTGGA CATCCCCTGT GAGCCTGCAG GAAGCCTTAT TAAAGAAGAA AAAACATTTT 
ATCGAAAGCT CTTCAAAACG AGTTGAACAA ATCAAGCAGA AAGCTGCGGC AGTCGGAAAC 
CCTGTATTTA ACTCCACGCC GAATGTTGGA GTCAGCGCGC AGGAGGAGGC GTCTGCCTGT 
GACGAAGCTT CAGCAAGTGA CAGCAGCACC CTGAAGAAGG TTGTAGAAGT TCGTGTGTGT 
ACCCCGGAGG AGCGCAAGCT GTCGGAGATA GAGATGCACC AGAGGACTAT AAGGTTGTAC 
AACCAGCTCG ACGAAGTTAA AACCAAAAAA GAGGAGAAGA TGAGAAAGGA GTCCTATGCC 
AGAAACAGAG AGAAGGCTAA CGAGTTTAAG AAGAAAACTC TGGAAAAGCT GAGATCCAGG 
AGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cep295 ( XM_031895776.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751636.1 Xenopus tropicalis centrosomal protein 295 (cep295), transcript variant X2, mRNA.

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CloneID	OXa51205
Clone ID Related Accession (Same CDS sequence)	XM_031895775.1 , XM_031895776.1 , XM_031895777.1
Accession Version	XM_031895776.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5349bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	centrosomal protein of 295 kDa isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACGCA AAGTTGCAAA TACCGGCGTC ATAAGACTTA GCCCCAATGA AGAAGCCTTG 
CTGCTAAAGC AGGAACGTGA AAGAAGGCGC AAACTCCGCT TACAACAGGT TAGAGAACAG 
GAGAAGTTCA TTGCCCTGCA AATCCGTCAC AATGTTAAAG AAAGGAGGGA ACAGGAGCTT 
CAGAATTTGG CAGAGGAGCT GCGCTTGGAG TGGCAGGCAT CGCAGGCTGA GAAGCTCCGA 
GCCCTGGAGA AGCTTTATGT GTCCAGTCTA AGTGCGATTG GAGAGGGGCA CAGGCAAGCC 
AAGGAAAATG AACCGGACCT TCAAGCCGTT GAGAGGCAAG CGGCAGCCAA TAGACAGAAA 
GCAGAGAAGC GGCACAGAGA AGCTCTCAGG GAATTAAAGC AGCACAAAGA AAAACAATTG 
AGAGAGCAAA CCTGGCTTTT GAAAGCCCGC AGGAAAGCGC TTATTACTGA GAAGGAGCGT 
GCTGCAAAGA TAGCAAGCCT TCCTCCCCCA CCCCCAGACC CTCTAGAGAA TATTGAGGTT 
TTGCGAAGGT TCCCAACCGT GAAGCTGTGC AATGTTGATG GCTTCTCTGT ATCTCATTAT 
CATCTGCCTG AACCATACGT AGACCGAGAG ACGGATACGG AACAGCCAGA TGCTCGGGTT 
GCCGCTGAAG AGGAAGCAAA GCGTTTGGAT GCCTTACAGA AAGAGGAAGA GAGAGAAAGG 
AGGGAAAGGA TGGAGAAGGC TCACTTACGG GGATCCCATG CCTTGAAAAA GGTTCTTTTA 
ACCCAAGACC GGGACAAACT GCTGAAAGAA CTGGAACAGA TGCAGCAAGA GGATTTGGCT 
CAGAGGAGGC AGGTTGTCGC CCAGATGCCT CAGCAGCTAT TTGAACCGGC CTATAGGCGA 
GAAGAGATAC AGGAGGAGTG GCAGAGGGAA CTGGAGGTTG CTTTTGAAGA CATGTACACT 
GGAGAAAGAA GGGTTAGAGG GGACATGGTT TTGCACCTCA AGCCACAGCC CTTACCAGTT 
CCATCTGCTG AAAGTTTTGA TGATGACCTG GACCTGTCTG TGGATCCAGA GCCCGTGTGT 
GAAACCCAGC CACAAACTGA TGCTGTTGAG GAACCAAGTC ACATTGAGAA AACGGATGAC 
AAAAGCCGCG AGCCACAATC GAGAATTGTT TTTAAGAGAC TGTTGAATAA AATACGAAGC 
CAGCAGGATC AGTGGAGCGC CAAAAGTGAT GTGGAGACGC AGAGCGACAC TGTGGAATCG 
GGATCTCTGC CTAATGAAAT ACATACAATC CATGCAGAAC TCCATGAGGC AAGGAAGCCT 
GCGCCTGAGG GCATTCAGAA CATGAGTGAT AGTACCATTC TAGCTGGTAA TGCAGTTCCT 
CGTCATGCAA CGGAGCCAGG AACACCAACA AAACAGGCCT CTGATTGGAA CAAGCAGGAT 
TTAGAGAGGC AGAAACAGGA GCAGCTGGAT TTGCTGAACA GACTAGAAGA GGAGAAAAAA 
CGCTTAGAAG CCGAATACTT GAGGGTTCAA CTCCAGATGC AAAAATCAAA GCAAGAAGGA 
ATGGAACCTC CAGAGGAGGA GGAGGAGGAA GAGGCAGCTG TAATTCCTCC TGCAGTGACT 
GAACCCAAAA CCATGGAAGA GCCCAAACCT GCGCAGGAAC CTCCAGGTTT AAGCCTAAAT 
GTTTCCACCG AGAGCCCGCA CATTCAGATA ATTCGACAGT ACCAGCAACG TCTAATTGAG 
CAAAACAGAC AACACAAGCA GTCGGTAGAA GAGGCGAGAC AACGTTTGCA AGAATACCAG 
CAATTGCTGA AAAAACGCTA TTCGCATTTG TCTGCCTCGA GGCTGCAGCC TGCAGCCAAG 
GAACCCGTTA GTTTCGCTAT AAAGCCCGGT CTCGCACAGA AACCTGCACA GCACTTGGAA 
GACATTGGCG GTGCTTCAGA CTCCCATGTT AGAGTAACCT CTTACCCTGG GGAGAGTCTA 
GCAGGGCACC TTGTATCTCT GGGTAGCTCT TTAATATCTG CAGTGCCGGT TTCTGGACAA 
AACTTATTTG CACCTTCACC AGTAAGCAGA ACAGCAGAGT TTGGACAGTC GGGAAGCAGT 
CCCCTCCCTC ATTCTACAGA GCAAGATAAC TTTATTCCAT ACTCTTCTGG GACTATTCCA 
ACACAACAAC AATATGGAGG GGCAGAAAGC ATGACCGGAA AAGACATTGC TGCGAACGGC 
ATTAAAAGTC CTAAAAGAGC AACACCGTTG GGAAATTTTA TTTTGGAAGT GGCAGCTCCC 
AGGCTAGAAC AATACACTTC TTCGCCCGAA GAAGACTCCA TCAGTCCTCC ATATTCTATG 
TACGATCTGG AAAAGCAGTC CTCCACATCA AGCAAAGGTG ATTATGATAC GGCCTCTGAT 
AGTTTCTACG CCCTTCCTTC TTCCTTGTCT TTGGGTCTGC CACAGAATAT AGAGAATATA 
GAGCAAGAAC CTCATGTTCA AAAAGTGGAT AGTGTTCCAT TCGGAGAGTT CTCTAATGTG 
CACGAGTTCC GCAAGGGGCT GATCTCTTCA ACGGCCAAGA TGCAAGCTCA GCAGACTCAG 
CTTAGGGATA TGCAGCTGCA GCTGGAGATG CAGAGGGAGT CCCTCTTGTC TAAACAGAAA 
TCCCAAGAAG AAGGTCTGTT ACAGAAACAG AAAGAGCTGG AAGATCAAAT GCGCAAGCAC 
CAAGAGTCGT TGGAACATTT CCTGGCCAAC AAGGAGTCTG CACAAGGGGC GTTACCTCCT 
GATCTCTGTC AGATTTCAAA TAACGAACGT CTTCGCCTTA TGTCTGCGCT GCTGAAAGCA 
CTGGGTGACG GTAAACAAGA GAACCTCAGT CTGGGAAATG GTAGTAGCCT TAGAGCTGCT 
GGGAGAGAAC TGAAAGGGAG ACCGTCTAAA CCTCCGGTTA CAAAAACCAA ATTGGGTCCA 
TCGTTGGAAC AACATGAGCT CAGTGCTATT CTGGAAGTGG AAACGCCAAG TGGCAGGCCT 
TCTAGTACTG GGCCGCCGGA ACACAAGAAC AGCAGCTCAG GCCGACAGGA TGGTGCTGAG 
GGTGGGAACT CGTCTGTGCC CCCTGCTTGC ATGCAAGAAA ACAGCAGACT GAGGGAAGCC 
GGTAACTATT CTCTCGGAGA TCCGGATCTT TCCAGAACAT CGACGAGCAG CAATGAAAGC 
AATAGGAGCC AGACTAAGTT ATCTTGGAGA CAGATGTTGT CTCTAGCAGC TGCTTCATCC 
CATAACTTGA CTCTCCCAGA TCAATCCTGG AGCTCTTCAG TGCACTCAGG CCTTTCTCCT 
CATCCTGCTG GTACCAAAAC AGCATTCCAC TTTCAGCAAC CTGCCCAGGA GAGATCCGAT 
TGGCTGGGGG CAGCTGAGCT GGGAAAAACA GGCATTGGAT ACAGTGCTCA AACGCAGGCC 
CGGGAAGCTT TCAGCGATCA CTTGTCAACA ACCACAATCT CTACGGGAAG TTTTCTGACC 
AGCGAGAAGG CCGATTCAAG CATTAGCAAC TTAGAGACTA TTTCAGACCT TCACAAATGC 
AACTTCTCCA TTATCAATGG CGCGGAACAC GGCAACTGGA GTACCTCAGT CAACGGGGTT 
CCACAGCCGA CTAGAAGGGA TGAGTTCCCG AGCCCGGAAT ATTTGTCGAC TGCACCGGAT 
CAGCGCAGCC ACATTCAGCA GATCATTGAT AAATACACCA GGGATCTCAG TGCTTCCCTA 
GAGAGAAATC TAAGCTTTCA TACTCCTACT GCTGCTCTAG ATGTATCTGG GAATGACAAT 
CACCTGCCCT CGGGTTTCTA TGCCCTGGAA CCGAAACCTG ACTTCAATGT CTCTACAACT 
TCCCATTCAC AATTAAACAA CACCGGCAGC TCTCAAGAGT CAAAGAACAC AAGCCAGAGC 
TCGTTTTACC CAAGCAGCAA AGAGTGGTCT TCAGAACAAT CTCATAGCTT CCAGTATCCA 
GACAGCAGCC ATAGTCCGAT GTCTTCCCCT GGCATAACTA GTGCGCAGCA CGTGGATGTG 
GAAATCACCC CTCATGTTCG TGGGGATCAA ACTCTAGGTT CCTCTGGATC GTTCCACCCT 
CTGCACCCAG AATGCACCCT TAATGAGCCA CCGCTGAGCA GCCCAGGTCT CTGCGGATGC 
AGCAGTGCAG TTGATGATCT GGGAAATGGA AGCATGAAGG CATCTAAACA ACATGCCTTA 
CAATCATCCA GTGTTGACTC TGGCAATTGG AACACAAACA GCAGCCCTGG TGCCTTTCAG 
CCTTCCGTGG AACCTCTCCG GGCCGATAGC CACACCTTGC TGGAGGATCT GGGCTCTTTT 
CATGAGCTGA TGGCCACAGA AACGACGGCT AATGAATCTG AGCTGTCTGA GCACCCACTC 
ACAGCTGTCG GCAAAACCAA ACATGGCGAC TCTCAGCAGC TTCATGAATT GCCTGTATTG 
TACGAAGCAC TCGAACCGGC AGAAATAGAG GCATCTGTAG GGGACTATCC AGGCCTGGGC 
TCCGTGCCAC AGCATTCCAA CGGTGATGAA GGGGCAGAAG TGGAGTCTTC ATCTCTGACC 
TTGAAAAGCG ACACACTTAC TGAGGCAGAA TCCAGCGTAC ACCCAGATAG TTCATCTGCC 
ATCAGCTCTT CTGCATTCTC TTTGGGCAGC TTTTCTCTTC TCAGGGACAC ACACAATGCC 
CGAGGCATCA TGGAAGAACC TGAGTTATCG CTAGTGAGTT TCCCTGACAG CAGCTTTGCT 
GGTTCAGAGC ACGTTACCAC CTTGACTAGC ATGTCGGATC ACCACGTGGA CCCAAGCTTT 
GCAGAGGGTT GCTTTCATCC CCTACCAGCT GAAGTGGACC ACTCCATGTT TAACATGCCA 
GACTGGTCAG CAGCGGGACC AAGCTTTACT GACCAATCTA CACCACAGCA CTTTGCAGCA 
CTTGATTGGA CATCCCCTGT GAGCCTGCAG GAAGCCTTAT TAAAGAAGAA AAAACATTTT 
ATCGAAAGCT CTTCAAAACG AGTTGAACAA ATCAAGCAGA AAGCTGCGGC AGTCGGAAAC 
CCTGTATTTA ACTCCACGCC GAATGTTGGA GTCAGCGCGC AGGAGGAGGC GTCTGCCTGT 
GACGAAGCTT CAGCAAGTGA CAGCAGCACC CTGAAGAAGG TTGTAGAAGT TCGTGTGTGT 
ACCCCGGAGG AGCGCAAGCT GTCGGAGATA GAGATGCACC AGAGGACTAT AAGGTTGTAC 
AACCAGCTCG ACGAAGTTAA AACCAAAAAA GAGGAGAAGA TGAGAAAGGA GTCCTATGCC 
AGAAACAGAG AGAAGGCTAA CGAGTTTAAG AAGAAAACTC TGGAAAAGCT GAGATCCAGG 
AGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751636.1
CDS	54..5402
Translation	MKRKVANTGVIRLSPNEEALLLKQERERRRKLRLQQVREQEKFIALQIRHNVKERREQELQNLAEELRLEWQASQAEKLRALEKLYVSSLSAIGEGHRQAKENEPDLQAVERQAAANRQKAEKRHREALRELKQHKEKQLREQTWLLKARRKALITEKERAAKIASLPPPPPDPLENIEVLRRFPTVKLCNVDGFSVSHYHLPEPYVDRETDTEQPDARVAAEEEAKRLDALQKEEERERRERMEKAHLRGSHALKKVLLTQDRDKLLKELEQMQQEDLAQRRQVVAQMPQQLFEPAYRREEIQEEWQRELEVAFEDMYTGERRVRGDMVLHLKPQPLPVPSAESFDDDLDLSVDPEPVCETQPQTDAVEEPSHIEKTDDKSREPQSRIVFKRLLNKIRSQQDQWSAKSDVETQSDTVESGSLPNEIHTIHAELHEARKPAPEGIQNMSDSTILAGNAVPRHATEPGTPTKQASDWNKQDLERQKQEQLDLLNRLEEEKKRLEAEYLRVQLQMQKSKQEGMEPPEEEEEEEAAVIPPAVTEPKTMEEPKPAQEPPGLSLNVSTESPHIQIIRQYQQRLIEQNRQHKQSVEEARQRLQEYQQLLKKRYSHLSASRLQPAAKEPVSFAIKPGLAQKPAQHLEDIGGASDSHVRVTSYPGESLAGHLVSLGSSLISAVPVSGQNLFAPSPVSRTAEFGQSGSSPLPHSTEQDNFIPYSSGTIPTQQQYGGAESMTGKDIAANGIKSPKRATPLGNFILEVAAPRLEQYTSSPEEDSISPPYSMYDLEKQSSTSSKGDYDTASDSFYALPSSLSLGLPQNIENIEQEPHVQKVDSVPFGEFSNVHEFRKGLISSTAKMQAQQTQLRDMQLQLEMQRESLLSKQKSQEEGLLQKQKELEDQMRKHQESLEHFLANKESAQGALPPDLCQISNNERLRLMSALLKALGDGKQENLSLGNGSSLRAAGRELKGRPSKPPVTKTKLGPSLEQHELSAILEVETPSGRPSSTGPPEHKNSSSGRQDGAEGGNSSVPPACMQENSRLREAGNYSLGDPDLSRTSTSSNESNRSQTKLSWRQMLSLAAASSHNLTLPDQSWSSSVHSGLSPHPAGTKTAFHFQQPAQERSDWLGAAELGKTGIGYSAQTQAREAFSDHLSTTTISTGSFLTSEKADSSISNLETISDLHKCNFSIINGAEHGNWSTSVNGVPQPTRRDEFPSPEYLSTAPDQRSHIQQIIDKYTRDLSASLERNLSFHTPTAALDVSGNDNHLPSGFYALEPKPDFNVSTTSHSQLNNTGSSQESKNTSQSSFYPSSKEWSSEQSHSFQYPDSSHSPMSSPGITSAQHVDVEITPHVRGDQTLGSSGSFHPLHPECTLNEPPLSSPGLCGCSSAVDDLGNGSMKASKQHALQSSSVDSGNWNTNSSPGAFQPSVEPLRADSHTLLEDLGSFHELMATETTANESELSEHPLTAVGKTKHGDSQQLHELPVLYEALEPAEIEASVGDYPGLGSVPQHSNGDEGAEVESSSLTLKSDTLTEAESSVHPDSSSAISSSAFSLGSFSLLRDTHNARGIMEEPELSLVSFPDSSFAGSEHVTTLTSMSDHHVDPSFAEGCFHPLPAEVDHSMFNMPDWSAAGPSFTDQSTPQHFAALDWTSPVSLQEALLKKKKHFIESSSKRVEQIKQKAAAVGNPVFNSTPNVGVSAQEEASACDEASASDSSTLKKVVEVRVCTPEERKLSEIEMHQRTIRLYNQLDEVKTKKEEKMRKESYARNREKANEFKKKTLEKLRSRRK

Target ORF information:

RefSeq Version	XM_031895776.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis centrosomal protein 295 (cep295), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895776.1

ORF Insert Sequence:

ATGAAACGCA AAGTTGCAAA TACCGGCGTC ATAAGACTTA GCCCCAATGA AGAAGCCTTG 
CTGCTAAAGC AGGAACGTGA AAGAAGGCGC AAACTCCGCT TACAACAGGT TAGAGAACAG 
GAGAAGTTCA TTGCCCTGCA AATCCGTCAC AATGTTAAAG AAAGGAGGGA ACAGGAGCTT 
CAGAATTTGG CAGAGGAGCT GCGCTTGGAG TGGCAGGCAT CGCAGGCTGA GAAGCTCCGA 
GCCCTGGAGA AGCTTTATGT GTCCAGTCTA AGTGCGATTG GAGAGGGGCA CAGGCAAGCC 
AAGGAAAATG AACCGGACCT TCAAGCCGTT GAGAGGCAAG CGGCAGCCAA TAGACAGAAA 
GCAGAGAAGC GGCACAGAGA AGCTCTCAGG GAATTAAAGC AGCACAAAGA AAAACAATTG 
AGAGAGCAAA CCTGGCTTTT GAAAGCCCGC AGGAAAGCGC TTATTACTGA GAAGGAGCGT 
GCTGCAAAGA TAGCAAGCCT TCCTCCCCCA CCCCCAGACC CTCTAGAGAA TATTGAGGTT 
TTGCGAAGGT TCCCAACCGT GAAGCTGTGC AATGTTGATG GCTTCTCTGT ATCTCATTAT 
CATCTGCCTG AACCATACGT AGACCGAGAG ACGGATACGG AACAGCCAGA TGCTCGGGTT 
GCCGCTGAAG AGGAAGCAAA GCGTTTGGAT GCCTTACAGA AAGAGGAAGA GAGAGAAAGG 
AGGGAAAGGA TGGAGAAGGC TCACTTACGG GGATCCCATG CCTTGAAAAA GGTTCTTTTA 
ACCCAAGACC GGGACAAACT GCTGAAAGAA CTGGAACAGA TGCAGCAAGA GGATTTGGCT 
CAGAGGAGGC AGGTTGTCGC CCAGATGCCT CAGCAGCTAT TTGAACCGGC CTATAGGCGA 
GAAGAGATAC AGGAGGAGTG GCAGAGGGAA CTGGAGGTTG CTTTTGAAGA CATGTACACT 
GGAGAAAGAA GGGTTAGAGG GGACATGGTT TTGCACCTCA AGCCACAGCC CTTACCAGTT 
CCATCTGCTG AAAGTTTTGA TGATGACCTG GACCTGTCTG TGGATCCAGA GCCCGTGTGT 
GAAACCCAGC CACAAACTGA TGCTGTTGAG GAACCAAGTC ACATTGAGAA AACGGATGAC 
AAAAGCCGCG AGCCACAATC GAGAATTGTT TTTAAGAGAC TGTTGAATAA AATACGAAGC 
CAGCAGGATC AGTGGAGCGC CAAAAGTGAT GTGGAGACGC AGAGCGACAC TGTGGAATCG 
GGATCTCTGC CTAATGAAAT ACATACAATC CATGCAGAAC TCCATGAGGC AAGGAAGCCT 
GCGCCTGAGG GCATTCAGAA CATGAGTGAT AGTACCATTC TAGCTGGTAA TGCAGTTCCT 
CGTCATGCAA CGGAGCCAGG AACACCAACA AAACAGGCCT CTGATTGGAA CAAGCAGGAT 
TTAGAGAGGC AGAAACAGGA GCAGCTGGAT TTGCTGAACA GACTAGAAGA GGAGAAAAAA 
CGCTTAGAAG CCGAATACTT GAGGGTTCAA CTCCAGATGC AAAAATCAAA GCAAGAAGGA 
ATGGAACCTC CAGAGGAGGA GGAGGAGGAA GAGGCAGCTG TAATTCCTCC TGCAGTGACT 
GAACCCAAAA CCATGGAAGA GCCCAAACCT GCGCAGGAAC CTCCAGGTTT AAGCCTAAAT 
GTTTCCACCG AGAGCCCGCA CATTCAGATA ATTCGACAGT ACCAGCAACG TCTAATTGAG 
CAAAACAGAC AACACAAGCA GTCGGTAGAA GAGGCGAGAC AACGTTTGCA AGAATACCAG 
CAATTGCTGA AAAAACGCTA TTCGCATTTG TCTGCCTCGA GGCTGCAGCC TGCAGCCAAG 
GAACCCGTTA GTTTCGCTAT AAAGCCCGGT CTCGCACAGA AACCTGCACA GCACTTGGAA 
GACATTGGCG GTGCTTCAGA CTCCCATGTT AGAGTAACCT CTTACCCTGG GGAGAGTCTA 
GCAGGGCACC TTGTATCTCT GGGTAGCTCT TTAATATCTG CAGTGCCGGT TTCTGGACAA 
AACTTATTTG CACCTTCACC AGTAAGCAGA ACAGCAGAGT TTGGACAGTC GGGAAGCAGT 
CCCCTCCCTC ATTCTACAGA GCAAGATAAC TTTATTCCAT ACTCTTCTGG GACTATTCCA 
ACACAACAAC AATATGGAGG GGCAGAAAGC ATGACCGGAA AAGACATTGC TGCGAACGGC 
ATTAAAAGTC CTAAAAGAGC AACACCGTTG GGAAATTTTA TTTTGGAAGT GGCAGCTCCC 
AGGCTAGAAC AATACACTTC TTCGCCCGAA GAAGACTCCA TCAGTCCTCC ATATTCTATG 
TACGATCTGG AAAAGCAGTC CTCCACATCA AGCAAAGGTG ATTATGATAC GGCCTCTGAT 
AGTTTCTACG CCCTTCCTTC TTCCTTGTCT TTGGGTCTGC CACAGAATAT AGAGAATATA 
GAGCAAGAAC CTCATGTTCA AAAAGTGGAT AGTGTTCCAT TCGGAGAGTT CTCTAATGTG 
CACGAGTTCC GCAAGGGGCT GATCTCTTCA ACGGCCAAGA TGCAAGCTCA GCAGACTCAG 
CTTAGGGATA TGCAGCTGCA GCTGGAGATG CAGAGGGAGT CCCTCTTGTC TAAACAGAAA 
TCCCAAGAAG AAGGTCTGTT ACAGAAACAG AAAGAGCTGG AAGATCAAAT GCGCAAGCAC 
CAAGAGTCGT TGGAACATTT CCTGGCCAAC AAGGAGTCTG CACAAGGGGC GTTACCTCCT 
GATCTCTGTC AGATTTCAAA TAACGAACGT CTTCGCCTTA TGTCTGCGCT GCTGAAAGCA 
CTGGGTGACG GTAAACAAGA GAACCTCAGT CTGGGAAATG GTAGTAGCCT TAGAGCTGCT 
GGGAGAGAAC TGAAAGGGAG ACCGTCTAAA CCTCCGGTTA CAAAAACCAA ATTGGGTCCA 
TCGTTGGAAC AACATGAGCT CAGTGCTATT CTGGAAGTGG AAACGCCAAG TGGCAGGCCT 
TCTAGTACTG GGCCGCCGGA ACACAAGAAC AGCAGCTCAG GCCGACAGGA TGGTGCTGAG 
GGTGGGAACT CGTCTGTGCC CCCTGCTTGC ATGCAAGAAA ACAGCAGACT GAGGGAAGCC 
GGTAACTATT CTCTCGGAGA TCCGGATCTT TCCAGAACAT CGACGAGCAG CAATGAAAGC 
AATAGGAGCC AGACTAAGTT ATCTTGGAGA CAGATGTTGT CTCTAGCAGC TGCTTCATCC 
CATAACTTGA CTCTCCCAGA TCAATCCTGG AGCTCTTCAG TGCACTCAGG CCTTTCTCCT 
CATCCTGCTG GTACCAAAAC AGCATTCCAC TTTCAGCAAC CTGCCCAGGA GAGATCCGAT 
TGGCTGGGGG CAGCTGAGCT GGGAAAAACA GGCATTGGAT ACAGTGCTCA AACGCAGGCC 
CGGGAAGCTT TCAGCGATCA CTTGTCAACA ACCACAATCT CTACGGGAAG TTTTCTGACC 
AGCGAGAAGG CCGATTCAAG CATTAGCAAC TTAGAGACTA TTTCAGACCT TCACAAATGC 
AACTTCTCCA TTATCAATGG CGCGGAACAC GGCAACTGGA GTACCTCAGT CAACGGGGTT 
CCACAGCCGA CTAGAAGGGA TGAGTTCCCG AGCCCGGAAT ATTTGTCGAC TGCACCGGAT 
CAGCGCAGCC ACATTCAGCA GATCATTGAT AAATACACCA GGGATCTCAG TGCTTCCCTA 
GAGAGAAATC TAAGCTTTCA TACTCCTACT GCTGCTCTAG ATGTATCTGG GAATGACAAT 
CACCTGCCCT CGGGTTTCTA TGCCCTGGAA CCGAAACCTG ACTTCAATGT CTCTACAACT 
TCCCATTCAC AATTAAACAA CACCGGCAGC TCTCAAGAGT CAAAGAACAC AAGCCAGAGC 
TCGTTTTACC CAAGCAGCAA AGAGTGGTCT TCAGAACAAT CTCATAGCTT CCAGTATCCA 
GACAGCAGCC ATAGTCCGAT GTCTTCCCCT GGCATAACTA GTGCGCAGCA CGTGGATGTG 
GAAATCACCC CTCATGTTCG TGGGGATCAA ACTCTAGGTT CCTCTGGATC GTTCCACCCT 
CTGCACCCAG AATGCACCCT TAATGAGCCA CCGCTGAGCA GCCCAGGTCT CTGCGGATGC 
AGCAGTGCAG TTGATGATCT GGGAAATGGA AGCATGAAGG CATCTAAACA ACATGCCTTA 
CAATCATCCA GTGTTGACTC TGGCAATTGG AACACAAACA GCAGCCCTGG TGCCTTTCAG 
CCTTCCGTGG AACCTCTCCG GGCCGATAGC CACACCTTGC TGGAGGATCT GGGCTCTTTT 
CATGAGCTGA TGGCCACAGA AACGACGGCT AATGAATCTG AGCTGTCTGA GCACCCACTC 
ACAGCTGTCG GCAAAACCAA ACATGGCGAC TCTCAGCAGC TTCATGAATT GCCTGTATTG 
TACGAAGCAC TCGAACCGGC AGAAATAGAG GCATCTGTAG GGGACTATCC AGGCCTGGGC 
TCCGTGCCAC AGCATTCCAA CGGTGATGAA GGGGCAGAAG TGGAGTCTTC ATCTCTGACC 
TTGAAAAGCG ACACACTTAC TGAGGCAGAA TCCAGCGTAC ACCCAGATAG TTCATCTGCC 
ATCAGCTCTT CTGCATTCTC TTTGGGCAGC TTTTCTCTTC TCAGGGACAC ACACAATGCC 
CGAGGCATCA TGGAAGAACC TGAGTTATCG CTAGTGAGTT TCCCTGACAG CAGCTTTGCT 
GGTTCAGAGC ACGTTACCAC CTTGACTAGC ATGTCGGATC ACCACGTGGA CCCAAGCTTT 
GCAGAGGGTT GCTTTCATCC CCTACCAGCT GAAGTGGACC ACTCCATGTT TAACATGCCA 
GACTGGTCAG CAGCGGGACC AAGCTTTACT GACCAATCTA CACCACAGCA CTTTGCAGCA 
CTTGATTGGA CATCCCCTGT GAGCCTGCAG GAAGCCTTAT TAAAGAAGAA AAAACATTTT 
ATCGAAAGCT CTTCAAAACG AGTTGAACAA ATCAAGCAGA AAGCTGCGGC AGTCGGAAAC 
CCTGTATTTA ACTCCACGCC GAATGTTGGA GTCAGCGCGC AGGAGGAGGC GTCTGCCTGT 
GACGAAGCTT CAGCAAGTGA CAGCAGCACC CTGAAGAAGG TTGTAGAAGT TCGTGTGTGT 
ACCCCGGAGG AGCGCAAGCT GTCGGAGATA GAGATGCACC AGAGGACTAT AAGGTTGTAC 
AACCAGCTCG ACGAAGTTAA AACCAAAAAA GAGGAGAAGA TGAGAAAGGA GTCCTATGCC 
AGAAACAGAG AGAAGGCTAA CGAGTTTAAG AAGAAAACTC TGGAAAAGCT GAGATCCAGG 
AGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cep295 ( XM_031895777.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751637.1 Xenopus tropicalis centrosomal protein 295 (cep295), transcript variant X3, mRNA.

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CloneID	OXa51205
Clone ID Related Accession (Same CDS sequence)	XM_031895775.1 , XM_031895776.1 , XM_031895777.1
Accession Version	XM_031895777.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5349bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	centrosomal protein of 295 kDa isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACGCA AAGTTGCAAA TACCGGCGTC ATAAGACTTA GCCCCAATGA AGAAGCCTTG 
CTGCTAAAGC AGGAACGTGA AAGAAGGCGC AAACTCCGCT TACAACAGGT TAGAGAACAG 
GAGAAGTTCA TTGCCCTGCA AATCCGTCAC AATGTTAAAG AAAGGAGGGA ACAGGAGCTT 
CAGAATTTGG CAGAGGAGCT GCGCTTGGAG TGGCAGGCAT CGCAGGCTGA GAAGCTCCGA 
GCCCTGGAGA AGCTTTATGT GTCCAGTCTA AGTGCGATTG GAGAGGGGCA CAGGCAAGCC 
AAGGAAAATG AACCGGACCT TCAAGCCGTT GAGAGGCAAG CGGCAGCCAA TAGACAGAAA 
GCAGAGAAGC GGCACAGAGA AGCTCTCAGG GAATTAAAGC AGCACAAAGA AAAACAATTG 
AGAGAGCAAA CCTGGCTTTT GAAAGCCCGC AGGAAAGCGC TTATTACTGA GAAGGAGCGT 
GCTGCAAAGA TAGCAAGCCT TCCTCCCCCA CCCCCAGACC CTCTAGAGAA TATTGAGGTT 
TTGCGAAGGT TCCCAACCGT GAAGCTGTGC AATGTTGATG GCTTCTCTGT ATCTCATTAT 
CATCTGCCTG AACCATACGT AGACCGAGAG ACGGATACGG AACAGCCAGA TGCTCGGGTT 
GCCGCTGAAG AGGAAGCAAA GCGTTTGGAT GCCTTACAGA AAGAGGAAGA GAGAGAAAGG 
AGGGAAAGGA TGGAGAAGGC TCACTTACGG GGATCCCATG CCTTGAAAAA GGTTCTTTTA 
ACCCAAGACC GGGACAAACT GCTGAAAGAA CTGGAACAGA TGCAGCAAGA GGATTTGGCT 
CAGAGGAGGC AGGTTGTCGC CCAGATGCCT CAGCAGCTAT TTGAACCGGC CTATAGGCGA 
GAAGAGATAC AGGAGGAGTG GCAGAGGGAA CTGGAGGTTG CTTTTGAAGA CATGTACACT 
GGAGAAAGAA GGGTTAGAGG GGACATGGTT TTGCACCTCA AGCCACAGCC CTTACCAGTT 
CCATCTGCTG AAAGTTTTGA TGATGACCTG GACCTGTCTG TGGATCCAGA GCCCGTGTGT 
GAAACCCAGC CACAAACTGA TGCTGTTGAG GAACCAAGTC ACATTGAGAA AACGGATGAC 
AAAAGCCGCG AGCCACAATC GAGAATTGTT TTTAAGAGAC TGTTGAATAA AATACGAAGC 
CAGCAGGATC AGTGGAGCGC CAAAAGTGAT GTGGAGACGC AGAGCGACAC TGTGGAATCG 
GGATCTCTGC CTAATGAAAT ACATACAATC CATGCAGAAC TCCATGAGGC AAGGAAGCCT 
GCGCCTGAGG GCATTCAGAA CATGAGTGAT AGTACCATTC TAGCTGGTAA TGCAGTTCCT 
CGTCATGCAA CGGAGCCAGG AACACCAACA AAACAGGCCT CTGATTGGAA CAAGCAGGAT 
TTAGAGAGGC AGAAACAGGA GCAGCTGGAT TTGCTGAACA GACTAGAAGA GGAGAAAAAA 
CGCTTAGAAG CCGAATACTT GAGGGTTCAA CTCCAGATGC AAAAATCAAA GCAAGAAGGA 
ATGGAACCTC CAGAGGAGGA GGAGGAGGAA GAGGCAGCTG TAATTCCTCC TGCAGTGACT 
GAACCCAAAA CCATGGAAGA GCCCAAACCT GCGCAGGAAC CTCCAGGTTT AAGCCTAAAT 
GTTTCCACCG AGAGCCCGCA CATTCAGATA ATTCGACAGT ACCAGCAACG TCTAATTGAG 
CAAAACAGAC AACACAAGCA GTCGGTAGAA GAGGCGAGAC AACGTTTGCA AGAATACCAG 
CAATTGCTGA AAAAACGCTA TTCGCATTTG TCTGCCTCGA GGCTGCAGCC TGCAGCCAAG 
GAACCCGTTA GTTTCGCTAT AAAGCCCGGT CTCGCACAGA AACCTGCACA GCACTTGGAA 
GACATTGGCG GTGCTTCAGA CTCCCATGTT AGAGTAACCT CTTACCCTGG GGAGAGTCTA 
GCAGGGCACC TTGTATCTCT GGGTAGCTCT TTAATATCTG CAGTGCCGGT TTCTGGACAA 
AACTTATTTG CACCTTCACC AGTAAGCAGA ACAGCAGAGT TTGGACAGTC GGGAAGCAGT 
CCCCTCCCTC ATTCTACAGA GCAAGATAAC TTTATTCCAT ACTCTTCTGG GACTATTCCA 
ACACAACAAC AATATGGAGG GGCAGAAAGC ATGACCGGAA AAGACATTGC TGCGAACGGC 
ATTAAAAGTC CTAAAAGAGC AACACCGTTG GGAAATTTTA TTTTGGAAGT GGCAGCTCCC 
AGGCTAGAAC AATACACTTC TTCGCCCGAA GAAGACTCCA TCAGTCCTCC ATATTCTATG 
TACGATCTGG AAAAGCAGTC CTCCACATCA AGCAAAGGTG ATTATGATAC GGCCTCTGAT 
AGTTTCTACG CCCTTCCTTC TTCCTTGTCT TTGGGTCTGC CACAGAATAT AGAGAATATA 
GAGCAAGAAC CTCATGTTCA AAAAGTGGAT AGTGTTCCAT TCGGAGAGTT CTCTAATGTG 
CACGAGTTCC GCAAGGGGCT GATCTCTTCA ACGGCCAAGA TGCAAGCTCA GCAGACTCAG 
CTTAGGGATA TGCAGCTGCA GCTGGAGATG CAGAGGGAGT CCCTCTTGTC TAAACAGAAA 
TCCCAAGAAG AAGGTCTGTT ACAGAAACAG AAAGAGCTGG AAGATCAAAT GCGCAAGCAC 
CAAGAGTCGT TGGAACATTT CCTGGCCAAC AAGGAGTCTG CACAAGGGGC GTTACCTCCT 
GATCTCTGTC AGATTTCAAA TAACGAACGT CTTCGCCTTA TGTCTGCGCT GCTGAAAGCA 
CTGGGTGACG GTAAACAAGA GAACCTCAGT CTGGGAAATG GTAGTAGCCT TAGAGCTGCT 
GGGAGAGAAC TGAAAGGGAG ACCGTCTAAA CCTCCGGTTA CAAAAACCAA ATTGGGTCCA 
TCGTTGGAAC AACATGAGCT CAGTGCTATT CTGGAAGTGG AAACGCCAAG TGGCAGGCCT 
TCTAGTACTG GGCCGCCGGA ACACAAGAAC AGCAGCTCAG GCCGACAGGA TGGTGCTGAG 
GGTGGGAACT CGTCTGTGCC CCCTGCTTGC ATGCAAGAAA ACAGCAGACT GAGGGAAGCC 
GGTAACTATT CTCTCGGAGA TCCGGATCTT TCCAGAACAT CGACGAGCAG CAATGAAAGC 
AATAGGAGCC AGACTAAGTT ATCTTGGAGA CAGATGTTGT CTCTAGCAGC TGCTTCATCC 
CATAACTTGA CTCTCCCAGA TCAATCCTGG AGCTCTTCAG TGCACTCAGG CCTTTCTCCT 
CATCCTGCTG GTACCAAAAC AGCATTCCAC TTTCAGCAAC CTGCCCAGGA GAGATCCGAT 
TGGCTGGGGG CAGCTGAGCT GGGAAAAACA GGCATTGGAT ACAGTGCTCA AACGCAGGCC 
CGGGAAGCTT TCAGCGATCA CTTGTCAACA ACCACAATCT CTACGGGAAG TTTTCTGACC 
AGCGAGAAGG CCGATTCAAG CATTAGCAAC TTAGAGACTA TTTCAGACCT TCACAAATGC 
AACTTCTCCA TTATCAATGG CGCGGAACAC GGCAACTGGA GTACCTCAGT CAACGGGGTT 
CCACAGCCGA CTAGAAGGGA TGAGTTCCCG AGCCCGGAAT ATTTGTCGAC TGCACCGGAT 
CAGCGCAGCC ACATTCAGCA GATCATTGAT AAATACACCA GGGATCTCAG TGCTTCCCTA 
GAGAGAAATC TAAGCTTTCA TACTCCTACT GCTGCTCTAG ATGTATCTGG GAATGACAAT 
CACCTGCCCT CGGGTTTCTA TGCCCTGGAA CCGAAACCTG ACTTCAATGT CTCTACAACT 
TCCCATTCAC AATTAAACAA CACCGGCAGC TCTCAAGAGT CAAAGAACAC AAGCCAGAGC 
TCGTTTTACC CAAGCAGCAA AGAGTGGTCT TCAGAACAAT CTCATAGCTT CCAGTATCCA 
GACAGCAGCC ATAGTCCGAT GTCTTCCCCT GGCATAACTA GTGCGCAGCA CGTGGATGTG 
GAAATCACCC CTCATGTTCG TGGGGATCAA ACTCTAGGTT CCTCTGGATC GTTCCACCCT 
CTGCACCCAG AATGCACCCT TAATGAGCCA CCGCTGAGCA GCCCAGGTCT CTGCGGATGC 
AGCAGTGCAG TTGATGATCT GGGAAATGGA AGCATGAAGG CATCTAAACA ACATGCCTTA 
CAATCATCCA GTGTTGACTC TGGCAATTGG AACACAAACA GCAGCCCTGG TGCCTTTCAG 
CCTTCCGTGG AACCTCTCCG GGCCGATAGC CACACCTTGC TGGAGGATCT GGGCTCTTTT 
CATGAGCTGA TGGCCACAGA AACGACGGCT AATGAATCTG AGCTGTCTGA GCACCCACTC 
ACAGCTGTCG GCAAAACCAA ACATGGCGAC TCTCAGCAGC TTCATGAATT GCCTGTATTG 
TACGAAGCAC TCGAACCGGC AGAAATAGAG GCATCTGTAG GGGACTATCC AGGCCTGGGC 
TCCGTGCCAC AGCATTCCAA CGGTGATGAA GGGGCAGAAG TGGAGTCTTC ATCTCTGACC 
TTGAAAAGCG ACACACTTAC TGAGGCAGAA TCCAGCGTAC ACCCAGATAG TTCATCTGCC 
ATCAGCTCTT CTGCATTCTC TTTGGGCAGC TTTTCTCTTC TCAGGGACAC ACACAATGCC 
CGAGGCATCA TGGAAGAACC TGAGTTATCG CTAGTGAGTT TCCCTGACAG CAGCTTTGCT 
GGTTCAGAGC ACGTTACCAC CTTGACTAGC ATGTCGGATC ACCACGTGGA CCCAAGCTTT 
GCAGAGGGTT GCTTTCATCC CCTACCAGCT GAAGTGGACC ACTCCATGTT TAACATGCCA 
GACTGGTCAG CAGCGGGACC AAGCTTTACT GACCAATCTA CACCACAGCA CTTTGCAGCA 
CTTGATTGGA CATCCCCTGT GAGCCTGCAG GAAGCCTTAT TAAAGAAGAA AAAACATTTT 
ATCGAAAGCT CTTCAAAACG AGTTGAACAA ATCAAGCAGA AAGCTGCGGC AGTCGGAAAC 
CCTGTATTTA ACTCCACGCC GAATGTTGGA GTCAGCGCGC AGGAGGAGGC GTCTGCCTGT 
GACGAAGCTT CAGCAAGTGA CAGCAGCACC CTGAAGAAGG TTGTAGAAGT TCGTGTGTGT 
ACCCCGGAGG AGCGCAAGCT GTCGGAGATA GAGATGCACC AGAGGACTAT AAGGTTGTAC 
AACCAGCTCG ACGAAGTTAA AACCAAAAAA GAGGAGAAGA TGAGAAAGGA GTCCTATGCC 
AGAAACAGAG AGAAGGCTAA CGAGTTTAAG AAGAAAACTC TGGAAAAGCT GAGATCCAGG 
AGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751637.1
CDS	94..5442
Translation	MKRKVANTGVIRLSPNEEALLLKQERERRRKLRLQQVREQEKFIALQIRHNVKERREQELQNLAEELRLEWQASQAEKLRALEKLYVSSLSAIGEGHRQAKENEPDLQAVERQAAANRQKAEKRHREALRELKQHKEKQLREQTWLLKARRKALITEKERAAKIASLPPPPPDPLENIEVLRRFPTVKLCNVDGFSVSHYHLPEPYVDRETDTEQPDARVAAEEEAKRLDALQKEEERERRERMEKAHLRGSHALKKVLLTQDRDKLLKELEQMQQEDLAQRRQVVAQMPQQLFEPAYRREEIQEEWQRELEVAFEDMYTGERRVRGDMVLHLKPQPLPVPSAESFDDDLDLSVDPEPVCETQPQTDAVEEPSHIEKTDDKSREPQSRIVFKRLLNKIRSQQDQWSAKSDVETQSDTVESGSLPNEIHTIHAELHEARKPAPEGIQNMSDSTILAGNAVPRHATEPGTPTKQASDWNKQDLERQKQEQLDLLNRLEEEKKRLEAEYLRVQLQMQKSKQEGMEPPEEEEEEEAAVIPPAVTEPKTMEEPKPAQEPPGLSLNVSTESPHIQIIRQYQQRLIEQNRQHKQSVEEARQRLQEYQQLLKKRYSHLSASRLQPAAKEPVSFAIKPGLAQKPAQHLEDIGGASDSHVRVTSYPGESLAGHLVSLGSSLISAVPVSGQNLFAPSPVSRTAEFGQSGSSPLPHSTEQDNFIPYSSGTIPTQQQYGGAESMTGKDIAANGIKSPKRATPLGNFILEVAAPRLEQYTSSPEEDSISPPYSMYDLEKQSSTSSKGDYDTASDSFYALPSSLSLGLPQNIENIEQEPHVQKVDSVPFGEFSNVHEFRKGLISSTAKMQAQQTQLRDMQLQLEMQRESLLSKQKSQEEGLLQKQKELEDQMRKHQESLEHFLANKESAQGALPPDLCQISNNERLRLMSALLKALGDGKQENLSLGNGSSLRAAGRELKGRPSKPPVTKTKLGPSLEQHELSAILEVETPSGRPSSTGPPEHKNSSSGRQDGAEGGNSSVPPACMQENSRLREAGNYSLGDPDLSRTSTSSNESNRSQTKLSWRQMLSLAAASSHNLTLPDQSWSSSVHSGLSPHPAGTKTAFHFQQPAQERSDWLGAAELGKTGIGYSAQTQAREAFSDHLSTTTISTGSFLTSEKADSSISNLETISDLHKCNFSIINGAEHGNWSTSVNGVPQPTRRDEFPSPEYLSTAPDQRSHIQQIIDKYTRDLSASLERNLSFHTPTAALDVSGNDNHLPSGFYALEPKPDFNVSTTSHSQLNNTGSSQESKNTSQSSFYPSSKEWSSEQSHSFQYPDSSHSPMSSPGITSAQHVDVEITPHVRGDQTLGSSGSFHPLHPECTLNEPPLSSPGLCGCSSAVDDLGNGSMKASKQHALQSSSVDSGNWNTNSSPGAFQPSVEPLRADSHTLLEDLGSFHELMATETTANESELSEHPLTAVGKTKHGDSQQLHELPVLYEALEPAEIEASVGDYPGLGSVPQHSNGDEGAEVESSSLTLKSDTLTEAESSVHPDSSSAISSSAFSLGSFSLLRDTHNARGIMEEPELSLVSFPDSSFAGSEHVTTLTSMSDHHVDPSFAEGCFHPLPAEVDHSMFNMPDWSAAGPSFTDQSTPQHFAALDWTSPVSLQEALLKKKKHFIESSSKRVEQIKQKAAAVGNPVFNSTPNVGVSAQEEASACDEASASDSSTLKKVVEVRVCTPEERKLSEIEMHQRTIRLYNQLDEVKTKKEEKMRKESYARNREKANEFKKKTLEKLRSRRK

Target ORF information:

RefSeq Version	XM_031895777.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis centrosomal protein 295 (cep295), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895777.1

ORF Insert Sequence:

ATGAAACGCA AAGTTGCAAA TACCGGCGTC ATAAGACTTA GCCCCAATGA AGAAGCCTTG 
CTGCTAAAGC AGGAACGTGA AAGAAGGCGC AAACTCCGCT TACAACAGGT TAGAGAACAG 
GAGAAGTTCA TTGCCCTGCA AATCCGTCAC AATGTTAAAG AAAGGAGGGA ACAGGAGCTT 
CAGAATTTGG CAGAGGAGCT GCGCTTGGAG TGGCAGGCAT CGCAGGCTGA GAAGCTCCGA 
GCCCTGGAGA AGCTTTATGT GTCCAGTCTA AGTGCGATTG GAGAGGGGCA CAGGCAAGCC 
AAGGAAAATG AACCGGACCT TCAAGCCGTT GAGAGGCAAG CGGCAGCCAA TAGACAGAAA 
GCAGAGAAGC GGCACAGAGA AGCTCTCAGG GAATTAAAGC AGCACAAAGA AAAACAATTG 
AGAGAGCAAA CCTGGCTTTT GAAAGCCCGC AGGAAAGCGC TTATTACTGA GAAGGAGCGT 
GCTGCAAAGA TAGCAAGCCT TCCTCCCCCA CCCCCAGACC CTCTAGAGAA TATTGAGGTT 
TTGCGAAGGT TCCCAACCGT GAAGCTGTGC AATGTTGATG GCTTCTCTGT ATCTCATTAT 
CATCTGCCTG AACCATACGT AGACCGAGAG ACGGATACGG AACAGCCAGA TGCTCGGGTT 
GCCGCTGAAG AGGAAGCAAA GCGTTTGGAT GCCTTACAGA AAGAGGAAGA GAGAGAAAGG 
AGGGAAAGGA TGGAGAAGGC TCACTTACGG GGATCCCATG CCTTGAAAAA GGTTCTTTTA 
ACCCAAGACC GGGACAAACT GCTGAAAGAA CTGGAACAGA TGCAGCAAGA GGATTTGGCT 
CAGAGGAGGC AGGTTGTCGC CCAGATGCCT CAGCAGCTAT TTGAACCGGC CTATAGGCGA 
GAAGAGATAC AGGAGGAGTG GCAGAGGGAA CTGGAGGTTG CTTTTGAAGA CATGTACACT 
GGAGAAAGAA GGGTTAGAGG GGACATGGTT TTGCACCTCA AGCCACAGCC CTTACCAGTT 
CCATCTGCTG AAAGTTTTGA TGATGACCTG GACCTGTCTG TGGATCCAGA GCCCGTGTGT 
GAAACCCAGC CACAAACTGA TGCTGTTGAG GAACCAAGTC ACATTGAGAA AACGGATGAC 
AAAAGCCGCG AGCCACAATC GAGAATTGTT TTTAAGAGAC TGTTGAATAA AATACGAAGC 
CAGCAGGATC AGTGGAGCGC CAAAAGTGAT GTGGAGACGC AGAGCGACAC TGTGGAATCG 
GGATCTCTGC CTAATGAAAT ACATACAATC CATGCAGAAC TCCATGAGGC AAGGAAGCCT 
GCGCCTGAGG GCATTCAGAA CATGAGTGAT AGTACCATTC TAGCTGGTAA TGCAGTTCCT 
CGTCATGCAA CGGAGCCAGG AACACCAACA AAACAGGCCT CTGATTGGAA CAAGCAGGAT 
TTAGAGAGGC AGAAACAGGA GCAGCTGGAT TTGCTGAACA GACTAGAAGA GGAGAAAAAA 
CGCTTAGAAG CCGAATACTT GAGGGTTCAA CTCCAGATGC AAAAATCAAA GCAAGAAGGA 
ATGGAACCTC CAGAGGAGGA GGAGGAGGAA GAGGCAGCTG TAATTCCTCC TGCAGTGACT 
GAACCCAAAA CCATGGAAGA GCCCAAACCT GCGCAGGAAC CTCCAGGTTT AAGCCTAAAT 
GTTTCCACCG AGAGCCCGCA CATTCAGATA ATTCGACAGT ACCAGCAACG TCTAATTGAG 
CAAAACAGAC AACACAAGCA GTCGGTAGAA GAGGCGAGAC AACGTTTGCA AGAATACCAG 
CAATTGCTGA AAAAACGCTA TTCGCATTTG TCTGCCTCGA GGCTGCAGCC TGCAGCCAAG 
GAACCCGTTA GTTTCGCTAT AAAGCCCGGT CTCGCACAGA AACCTGCACA GCACTTGGAA 
GACATTGGCG GTGCTTCAGA CTCCCATGTT AGAGTAACCT CTTACCCTGG GGAGAGTCTA 
GCAGGGCACC TTGTATCTCT GGGTAGCTCT TTAATATCTG CAGTGCCGGT TTCTGGACAA 
AACTTATTTG CACCTTCACC AGTAAGCAGA ACAGCAGAGT TTGGACAGTC GGGAAGCAGT 
CCCCTCCCTC ATTCTACAGA GCAAGATAAC TTTATTCCAT ACTCTTCTGG GACTATTCCA 
ACACAACAAC AATATGGAGG GGCAGAAAGC ATGACCGGAA AAGACATTGC TGCGAACGGC 
ATTAAAAGTC CTAAAAGAGC AACACCGTTG GGAAATTTTA TTTTGGAAGT GGCAGCTCCC 
AGGCTAGAAC AATACACTTC TTCGCCCGAA GAAGACTCCA TCAGTCCTCC ATATTCTATG 
TACGATCTGG AAAAGCAGTC CTCCACATCA AGCAAAGGTG ATTATGATAC GGCCTCTGAT 
AGTTTCTACG CCCTTCCTTC TTCCTTGTCT TTGGGTCTGC CACAGAATAT AGAGAATATA 
GAGCAAGAAC CTCATGTTCA AAAAGTGGAT AGTGTTCCAT TCGGAGAGTT CTCTAATGTG 
CACGAGTTCC GCAAGGGGCT GATCTCTTCA ACGGCCAAGA TGCAAGCTCA GCAGACTCAG 
CTTAGGGATA TGCAGCTGCA GCTGGAGATG CAGAGGGAGT CCCTCTTGTC TAAACAGAAA 
TCCCAAGAAG AAGGTCTGTT ACAGAAACAG AAAGAGCTGG AAGATCAAAT GCGCAAGCAC 
CAAGAGTCGT TGGAACATTT CCTGGCCAAC AAGGAGTCTG CACAAGGGGC GTTACCTCCT 
GATCTCTGTC AGATTTCAAA TAACGAACGT CTTCGCCTTA TGTCTGCGCT GCTGAAAGCA 
CTGGGTGACG GTAAACAAGA GAACCTCAGT CTGGGAAATG GTAGTAGCCT TAGAGCTGCT 
GGGAGAGAAC TGAAAGGGAG ACCGTCTAAA CCTCCGGTTA CAAAAACCAA ATTGGGTCCA 
TCGTTGGAAC AACATGAGCT CAGTGCTATT CTGGAAGTGG AAACGCCAAG TGGCAGGCCT 
TCTAGTACTG GGCCGCCGGA ACACAAGAAC AGCAGCTCAG GCCGACAGGA TGGTGCTGAG 
GGTGGGAACT CGTCTGTGCC CCCTGCTTGC ATGCAAGAAA ACAGCAGACT GAGGGAAGCC 
GGTAACTATT CTCTCGGAGA TCCGGATCTT TCCAGAACAT CGACGAGCAG CAATGAAAGC 
AATAGGAGCC AGACTAAGTT ATCTTGGAGA CAGATGTTGT CTCTAGCAGC TGCTTCATCC 
CATAACTTGA CTCTCCCAGA TCAATCCTGG AGCTCTTCAG TGCACTCAGG CCTTTCTCCT 
CATCCTGCTG GTACCAAAAC AGCATTCCAC TTTCAGCAAC CTGCCCAGGA GAGATCCGAT 
TGGCTGGGGG CAGCTGAGCT GGGAAAAACA GGCATTGGAT ACAGTGCTCA AACGCAGGCC 
CGGGAAGCTT TCAGCGATCA CTTGTCAACA ACCACAATCT CTACGGGAAG TTTTCTGACC 
AGCGAGAAGG CCGATTCAAG CATTAGCAAC TTAGAGACTA TTTCAGACCT TCACAAATGC 
AACTTCTCCA TTATCAATGG CGCGGAACAC GGCAACTGGA GTACCTCAGT CAACGGGGTT 
CCACAGCCGA CTAGAAGGGA TGAGTTCCCG AGCCCGGAAT ATTTGTCGAC TGCACCGGAT 
CAGCGCAGCC ACATTCAGCA GATCATTGAT AAATACACCA GGGATCTCAG TGCTTCCCTA 
GAGAGAAATC TAAGCTTTCA TACTCCTACT GCTGCTCTAG ATGTATCTGG GAATGACAAT 
CACCTGCCCT CGGGTTTCTA TGCCCTGGAA CCGAAACCTG ACTTCAATGT CTCTACAACT 
TCCCATTCAC AATTAAACAA CACCGGCAGC TCTCAAGAGT CAAAGAACAC AAGCCAGAGC 
TCGTTTTACC CAAGCAGCAA AGAGTGGTCT TCAGAACAAT CTCATAGCTT CCAGTATCCA 
GACAGCAGCC ATAGTCCGAT GTCTTCCCCT GGCATAACTA GTGCGCAGCA CGTGGATGTG 
GAAATCACCC CTCATGTTCG TGGGGATCAA ACTCTAGGTT CCTCTGGATC GTTCCACCCT 
CTGCACCCAG AATGCACCCT TAATGAGCCA CCGCTGAGCA GCCCAGGTCT CTGCGGATGC 
AGCAGTGCAG TTGATGATCT GGGAAATGGA AGCATGAAGG CATCTAAACA ACATGCCTTA 
CAATCATCCA GTGTTGACTC TGGCAATTGG AACACAAACA GCAGCCCTGG TGCCTTTCAG 
CCTTCCGTGG AACCTCTCCG GGCCGATAGC CACACCTTGC TGGAGGATCT GGGCTCTTTT 
CATGAGCTGA TGGCCACAGA AACGACGGCT AATGAATCTG AGCTGTCTGA GCACCCACTC 
ACAGCTGTCG GCAAAACCAA ACATGGCGAC TCTCAGCAGC TTCATGAATT GCCTGTATTG 
TACGAAGCAC TCGAACCGGC AGAAATAGAG GCATCTGTAG GGGACTATCC AGGCCTGGGC 
TCCGTGCCAC AGCATTCCAA CGGTGATGAA GGGGCAGAAG TGGAGTCTTC ATCTCTGACC 
TTGAAAAGCG ACACACTTAC TGAGGCAGAA TCCAGCGTAC ACCCAGATAG TTCATCTGCC 
ATCAGCTCTT CTGCATTCTC TTTGGGCAGC TTTTCTCTTC TCAGGGACAC ACACAATGCC 
CGAGGCATCA TGGAAGAACC TGAGTTATCG CTAGTGAGTT TCCCTGACAG CAGCTTTGCT 
GGTTCAGAGC ACGTTACCAC CTTGACTAGC ATGTCGGATC ACCACGTGGA CCCAAGCTTT 
GCAGAGGGTT GCTTTCATCC CCTACCAGCT GAAGTGGACC ACTCCATGTT TAACATGCCA 
GACTGGTCAG CAGCGGGACC AAGCTTTACT GACCAATCTA CACCACAGCA CTTTGCAGCA 
CTTGATTGGA CATCCCCTGT GAGCCTGCAG GAAGCCTTAT TAAAGAAGAA AAAACATTTT 
ATCGAAAGCT CTTCAAAACG AGTTGAACAA ATCAAGCAGA AAGCTGCGGC AGTCGGAAAC 
CCTGTATTTA ACTCCACGCC GAATGTTGGA GTCAGCGCGC AGGAGGAGGC GTCTGCCTGT 
GACGAAGCTT CAGCAAGTGA CAGCAGCACC CTGAAGAAGG TTGTAGAAGT TCGTGTGTGT 
ACCCCGGAGG AGCGCAAGCT GTCGGAGATA GAGATGCACC AGAGGACTAT AAGGTTGTAC 
AACCAGCTCG ACGAAGTTAA AACCAAAAAA GAGGAGAAGA TGAGAAAGGA GTCCTATGCC 
AGAAACAGAG AGAAGGCTAA CGAGTTTAAG AAGAAAACTC TGGAAAAGCT GAGATCCAGG 
AGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cep295 ( XM_031895778.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751638.1 Xenopus tropicalis centrosomal protein 295 (cep295), transcript variant X4, mRNA.

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CloneID	OXa51206
Clone ID Related Accession (Same CDS sequence)	XM_031895778.1
Accession Version	XM_031895778.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	centrosomal protein of 295 kDa isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACGCA AAGTTGCAAA TACCGGCGTC ATAAGACTTA GCCCCAATGA AGAAGCCTTG 
CTGCTAAAGC AGGAACGTGA AAGAAGGCGC AAACTCCGCT TACAACAGGT TAGAGAACAG 
GAGAAGTTCA TTGCCCTGCA AATCCGTCAC AATGTTAAAG AAAGGAGGGA ACAGGAGCTT 
CAGAATTTGG CAGAGGAGCT GCGCTTGGAG TGGCAGGCAT CGCAGGCTGA GAAGCTCCGA 
GCCCTGGAGA AGCTTTATGT GTCCAGTCTA AGTGCGATTG GAGAGGGGCA CAGGCAAGCC 
AAGGAAAATG AACCGGACCT TCAAGCCGTT GAGAGGCAAG CGGCAGCCAA TAGACAGAAA 
GCAGAGAAGC GGCACAGAGA AGCTCTCAGG GAATTAAAGC AGCACAAAGA AAAACAATTG 
AGAGAGCAAA CCTGGCTTTT GAAAGCCCGC AGGAAAGCGC TTATTACTGA GAAGGAGCGT 
GCTGCAAAGA TAGCAAGCCT TCCTCCCCCA CCCCCAGACC CTCTAGAGAA TATTGAGGTT 
TTGCGAAGGT TCCCAACCGT GAAGCTGTGC AATGTTGATG GCTTCTCTGT ATCTCATTAT 
CATCTGCCTG AACCATACGT AGACCGAGAG ACGGATACGG AACAGCCAGA TGCTCGGGTT 
GCCGCTGAAG AGGAAGCAAA GCGTTTGGAT GCCTTACAGA AAGAGGAAGA GAGAGAAAGG 
AGGGAAAGGA TGGAGAAGGC TCACTTACGG GGATCCCATG CCTTGAAAAA GGTTCTTTTA 
ACCCAAGACC GGGACAAACT GCTGAAAGAA CTGGAACAGA TGCAGCAAGA GGATTTGGCT 
CAGAGGAGGC AGGTTGTCGC CCAGATGCCT CAGCAGCTAT TTGAACCGGC CTATAGGCGA 
GAAGAGATAC AGGAGGAGTG GCAGAGGGAA CTGGAGGTTG CTTTTGAAGA CATGTACACT 
GGAGAAAGAA GGGTTAGAGG GGACATGGTT TTGCACCTCA AGCCACAGCC CTTACCAGTT 
CCATCTGCTG AAAGTTTTGA TGATGACCTG GACCTGTCTG TGGATCCAGA GCCCGTGTGT 
GAAACCCAGC CACAAACTGA TGCTGTTGAG GAACCAAGTC ACATTGAGAA AACGGATGAC 
AAAAGCCGCG AGCCACAATC GAGAATTGTT TTTAAGAGAC TGTTGAATAA AATACGAAGC 
CAGCAGGATC AGTGGAGCGC CAAAAGTGAT GTGGAGACGC AGAGCGACAC TGTGGAATCG 
GGATCTCTGC CTAATGAAAT ACATACAATC CATGCAGAAC TCCATGAGGC AAGGAAGCCT 
GCGCCTGAGG GCATTCAGAA CATGAGTGAT AGTACCATTC TAGCTGGTAA TGCAGTTCCT 
CGTCATGCAA CGGAGCCAGG AACACCAACA AAACAGGCCT CTGATTGGAA CAAGCAGGAT 
TTAGAGAGGC AGAAACAGGA GCAGCTGGAT TTGCTGAACA GACTAGAAGA GGAGAAAAAA 
CGCTTAGAAG CCGAATACTT GAGGGTTCAA CTCCAGATGC AAAAATCAAA GCAAGAAGGA 
ATGGAACCTC CAGAGGAGGA GGAGGAGGAA GAGGCAGCTG TAATTCCTCC TGCAGTGACT 
GAACCCAAAA CCATGGAAGA GCCCAAACCT GCGGAACCTC CAGGTTTAAG CCTAAATGTT 
TCCACCGAGA GCCCGCACAT TCAGATAATT CGACAGTACC AGCAACGTCT AATTGAGCAA 
AACAGACAAC ACAAGCAGTC GGTAGAAGAG GCGAGACAAC GTTTGCAAGA ATACCAGCAA 
TTGCTGAAAA AACGCTATTC GCATTTGTCT GCCTCGAGGC TGCAGCCTGC AGCCAAGGAA 
CCCGTTAGTT TCGCTATAAA GCCCGGTCTC GCACAGAAAC CTGCACAGCA CTTGGAAGAC 
ATTGGCGGTG CTTCAGACTC CCATGTTAGA GTAACCTCTT ACCCTGGGGA GAGTCTAGCA 
GGGCACCTTG TATCTCTGGG TAGCTCTTTA ATATCTGCAG TGCCGGTTTC TGGACAAAAC 
TTATTTGCAC CTTCACCAGT AAGCAGAACA GCAGAGTTTG GACAGTCGGG AAGCAGTCCC 
CTCCCTCATT CTACAGAGCA AGATAACTTT ATTCCATACT CTTCTGGGAC TATTCCAACA 
CAACAACAAT ATGGAGGGGC AGAAAGCATG ACCGGAAAAG ACATTGCTGC GAACGGCATT 
AAAAGTCCTA AAAGAGCAAC ACCGTTGGGA AATTTTATTT TGGAAGTGGC AGCTCCCAGG 
CTAGAACAAT ACACTTCTTC GCCCGAAGAA GACTCCATCA GTCCTCCATA TTCTATGTAC 
GATCTGGAAA AGCAGTCCTC CACATCAAGC AAAGGTGATT ATGATACGGC CTCTGATAGT 
TTCTACGCCC TTCCTTCTTC CTTGTCTTTG GGTCTGCCAC AGAATATAGA GAATATAGAG 
CAAGAACCTC ATGTTCAAAA AGTGGATAGT GTTCCATTCG GAGAGTTCTC TAATGTGCAC 
GAGTTCCGCA AGGGGCTGAT CTCTTCAACG GCCAAGATGC AAGCTCAGCA GACTCAGCTT 
AGGGATATGC AGCTGCAGCT GGAGATGCAG AGGGAGTCCC TCTTGTCTAA ACAGAAATCC 
CAAGAAGAAG GTCTGTTACA GAAACAGAAA GAGCTGGAAG ATCAAATGCG CAAGCACCAA 
GAGTCGTTGG AACATTTCCT GGCCAACAAG GAGTCTGCAC AAGGGGCGTT ACCTCCTGAT 
CTCTGTCAGA TTTCAAATAA CGAACGTCTT CGCCTTATGT CTGCGCTGCT GAAAGCACTG 
GGTGACGGTA AACAAGAGAA CCTCAGTCTG GGAAATGGTA GTAGCCTTAG AGCTGCTGGG 
AGAGAACTGA AAGGGAGACC GTCTAAACCT CCGGTTACAA AAACCAAATT GGGTCCATCG 
TTGGAACAAC ATGAGCTCAG TGCTATTCTG GAAGTGGAAA CGCCAAGTGG CAGGCCTTCT 
AGTACTGGGC CGCCGGAACA CAAGAACAGC AGCTCAGGCC GACAGGATGG TGCTGAGGGT 
GGGAACTCGT CTGTGCCCCC TGCTTGCATG CAAGAAAACA GCAGACTGAG GGAAGCCGGT 
AACTATTCTC TCGGAGATCC GGATCTTTCC AGAACATCGA CGAGCAGCAA TGAAAGCAAT 
AGGAGCCAGA CTAAGTTATC TTGGAGACAG ATGTTGTCTC TAGCAGCTGC TTCATCCCAT 
AACTTGACTC TCCCAGATCA ATCCTGGAGC TCTTCAGTGC ACTCAGGCCT TTCTCCTCAT 
CCTGCTGGTA CCAAAACAGC ATTCCACTTT CAGCAACCTG CCCAGGAGAG ATCCGATTGG 
CTGGGGGCAG CTGAGCTGGG AAAAACAGGC ATTGGATACA GTGCTCAAAC GCAGGCCCGG 
GAAGCTTTCA GCGATCACTT GTCAACAACC ACAATCTCTA CGGGAAGTTT TCTGACCAGC 
GAGAAGGCCG ATTCAAGCAT TAGCAACTTA GAGACTATTT CAGACCTTCA CAAATGCAAC 
TTCTCCATTA TCAATGGCGC GGAACACGGC AACTGGAGTA CCTCAGTCAA CGGGGTTCCA 
CAGCCGACTA GAAGGGATGA GTTCCCGAGC CCGGAATATT TGTCGACTGC ACCGGATCAG 
CGCAGCCACA TTCAGCAGAT CATTGATAAA TACACCAGGG ATCTCAGTGC TTCCCTAGAG 
AGAAATCTAA GCTTTCATAC TCCTACTGCT GCTCTAGATG TATCTGGGAA TGACAATCAC 
CTGCCCTCGG GTTTCTATGC CCTGGAACCG AAACCTGACT TCAATGTCTC TACAACTTCC 
CATTCACAAT TAAACAACAC CGGCAGCTCT CAAGAGTCAA AGAACACAAG CCAGAGCTCG 
TTTTACCCAA GCAGCAAAGA GTGGTCTTCA GAACAATCTC ATAGCTTCCA GTATCCAGAC 
AGCAGCCATA GTCCGATGTC TTCCCCTGGC ATAACTAGTG CGCAGCACGT GGATGTGGAA 
ATCACCCCTC ATGTTCGTGG GGATCAAACT CTAGGTTCCT CTGGATCGTT CCACCCTCTG 
CACCCAGAAT GCACCCTTAA TGAGCCACCG CTGAGCAGCC CAGGTCTCTG CGGATGCAGC 
AGTGCAGTTG ATGATCTGGG AAATGGAAGC ATGAAGGCAT CTAAACAACA TGCCTTACAA 
TCATCCAGTG TTGACTCTGG CAATTGGAAC ACAAACAGCA GCCCTGGTGC CTTTCAGCCT 
TCCGTGGAAC CTCTCCGGGC CGATAGCCAC ACCTTGCTGG AGGATCTGGG CTCTTTTCAT 
GAGCTGATGG CCACAGAAAC GACGGCTAAT GAATCTGAGC TGTCTGAGCA CCCACTCACA 
GCTGTCGGCA AAACCAAACA TGGCGACTCT CAGCAGCTTC ATGAATTGCC TGTATTGTAC 
GAAGCACTCG AACCGGCAGA AATAGAGGCA TCTGTAGGGG ACTATCCAGG CCTGGGCTCC 
GTGCCACAGC ATTCCAACGG TGATGAAGGG GCAGAAGTGG AGTCTTCATC TCTGACCTTG 
AAAAGCGACA CACTTACTGA GGCAGAATCC AGCGTACACC CAGATAGTTC ATCTGCCATC 
AGCTCTTCTG CATTCTCTTT GGGCAGCTTT TCTCTTCTCA GGGACACACA CAATGCCCGA 
GGCATCATGG AAGAACCTGA GTTATCGCTA GTGAGTTTCC CTGACAGCAG CTTTGCTGGT 
TCAGAGCACG TTACCACCTT GACTAGCATG TCGGATCACC ACGTGGACCC AAGCTTTGCA 
GAGGGTTGCT TTCATCCCCT ACCAGCTGAA GTGGACCACT CCATGTTTAA CATGCCAGAC 
TGGTCAGCAG CGGGACCAAG CTTTACTGAC CAATCTACAC CACAGCACTT TGCAGCACTT 
GATTGGACAT CCCCTGTGAG CCTGCAGGAA GCCTTATTAA AGAAGAAAAA ACATTTTATC 
GAAAGCTCTT CAAAACGAGT TGAACAAATC AAGCAGAAAG CTGCGGCAGT CGGAAACCCT 
GTATTTAACT CCACGCCGAA TGTTGGAGTC AGCGCGCAGG AGGAGGCGTC TGCCTGTGAC 
GAAGCTTCAG CAAGTGACAG CAGCACCCTG AAGAAGGTTG TAGAAGTTCG TGTGTGTACC 
CCGGAGGAGC GCAAGCTGTC GGAGATAGAG ATGCACCAGA GGACTATAAG GTTGTACAAC 
CAGCTCGACG AAGTTAAAAC CAAAAAAGAG GAGAAGATGA GAAAGGAGTC CTATGCCAGA 
AACAGAGAGA AGGCTAACGA GTTTAAGAAG AAAACTCTGG AAAAGCTGAG ATCCAGGAGG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751638.1
CDS	140..5485
Translation	MKRKVANTGVIRLSPNEEALLLKQERERRRKLRLQQVREQEKFIALQIRHNVKERREQELQNLAEELRLEWQASQAEKLRALEKLYVSSLSAIGEGHRQAKENEPDLQAVERQAAANRQKAEKRHREALRELKQHKEKQLREQTWLLKARRKALITEKERAAKIASLPPPPPDPLENIEVLRRFPTVKLCNVDGFSVSHYHLPEPYVDRETDTEQPDARVAAEEEAKRLDALQKEEERERRERMEKAHLRGSHALKKVLLTQDRDKLLKELEQMQQEDLAQRRQVVAQMPQQLFEPAYRREEIQEEWQRELEVAFEDMYTGERRVRGDMVLHLKPQPLPVPSAESFDDDLDLSVDPEPVCETQPQTDAVEEPSHIEKTDDKSREPQSRIVFKRLLNKIRSQQDQWSAKSDVETQSDTVESGSLPNEIHTIHAELHEARKPAPEGIQNMSDSTILAGNAVPRHATEPGTPTKQASDWNKQDLERQKQEQLDLLNRLEEEKKRLEAEYLRVQLQMQKSKQEGMEPPEEEEEEEAAVIPPAVTEPKTMEEPKPAEPPGLSLNVSTESPHIQIIRQYQQRLIEQNRQHKQSVEEARQRLQEYQQLLKKRYSHLSASRLQPAAKEPVSFAIKPGLAQKPAQHLEDIGGASDSHVRVTSYPGESLAGHLVSLGSSLISAVPVSGQNLFAPSPVSRTAEFGQSGSSPLPHSTEQDNFIPYSSGTIPTQQQYGGAESMTGKDIAANGIKSPKRATPLGNFILEVAAPRLEQYTSSPEEDSISPPYSMYDLEKQSSTSSKGDYDTASDSFYALPSSLSLGLPQNIENIEQEPHVQKVDSVPFGEFSNVHEFRKGLISSTAKMQAQQTQLRDMQLQLEMQRESLLSKQKSQEEGLLQKQKELEDQMRKHQESLEHFLANKESAQGALPPDLCQISNNERLRLMSALLKALGDGKQENLSLGNGSSLRAAGRELKGRPSKPPVTKTKLGPSLEQHELSAILEVETPSGRPSSTGPPEHKNSSSGRQDGAEGGNSSVPPACMQENSRLREAGNYSLGDPDLSRTSTSSNESNRSQTKLSWRQMLSLAAASSHNLTLPDQSWSSSVHSGLSPHPAGTKTAFHFQQPAQERSDWLGAAELGKTGIGYSAQTQAREAFSDHLSTTTISTGSFLTSEKADSSISNLETISDLHKCNFSIINGAEHGNWSTSVNGVPQPTRRDEFPSPEYLSTAPDQRSHIQQIIDKYTRDLSASLERNLSFHTPTAALDVSGNDNHLPSGFYALEPKPDFNVSTTSHSQLNNTGSSQESKNTSQSSFYPSSKEWSSEQSHSFQYPDSSHSPMSSPGITSAQHVDVEITPHVRGDQTLGSSGSFHPLHPECTLNEPPLSSPGLCGCSSAVDDLGNGSMKASKQHALQSSSVDSGNWNTNSSPGAFQPSVEPLRADSHTLLEDLGSFHELMATETTANESELSEHPLTAVGKTKHGDSQQLHELPVLYEALEPAEIEASVGDYPGLGSVPQHSNGDEGAEVESSSLTLKSDTLTEAESSVHPDSSSAISSSAFSLGSFSLLRDTHNARGIMEEPELSLVSFPDSSFAGSEHVTTLTSMSDHHVDPSFAEGCFHPLPAEVDHSMFNMPDWSAAGPSFTDQSTPQHFAALDWTSPVSLQEALLKKKKHFIESSSKRVEQIKQKAAAVGNPVFNSTPNVGVSAQEEASACDEASASDSSTLKKVVEVRVCTPEERKLSEIEMHQRTIRLYNQLDEVKTKKEEKMRKESYARNREKANEFKKKTLEKLRSRRK

Target ORF information:

RefSeq Version	XM_031895778.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis centrosomal protein 295 (cep295), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895778.1

ORF Insert Sequence:

ATGAAACGCA AAGTTGCAAA TACCGGCGTC ATAAGACTTA GCCCCAATGA AGAAGCCTTG 
CTGCTAAAGC AGGAACGTGA AAGAAGGCGC AAACTCCGCT TACAACAGGT TAGAGAACAG 
GAGAAGTTCA TTGCCCTGCA AATCCGTCAC AATGTTAAAG AAAGGAGGGA ACAGGAGCTT 
CAGAATTTGG CAGAGGAGCT GCGCTTGGAG TGGCAGGCAT CGCAGGCTGA GAAGCTCCGA 
GCCCTGGAGA AGCTTTATGT GTCCAGTCTA AGTGCGATTG GAGAGGGGCA CAGGCAAGCC 
AAGGAAAATG AACCGGACCT TCAAGCCGTT GAGAGGCAAG CGGCAGCCAA TAGACAGAAA 
GCAGAGAAGC GGCACAGAGA AGCTCTCAGG GAATTAAAGC AGCACAAAGA AAAACAATTG 
AGAGAGCAAA CCTGGCTTTT GAAAGCCCGC AGGAAAGCGC TTATTACTGA GAAGGAGCGT 
GCTGCAAAGA TAGCAAGCCT TCCTCCCCCA CCCCCAGACC CTCTAGAGAA TATTGAGGTT 
TTGCGAAGGT TCCCAACCGT GAAGCTGTGC AATGTTGATG GCTTCTCTGT ATCTCATTAT 
CATCTGCCTG AACCATACGT AGACCGAGAG ACGGATACGG AACAGCCAGA TGCTCGGGTT 
GCCGCTGAAG AGGAAGCAAA GCGTTTGGAT GCCTTACAGA AAGAGGAAGA GAGAGAAAGG 
AGGGAAAGGA TGGAGAAGGC TCACTTACGG GGATCCCATG CCTTGAAAAA GGTTCTTTTA 
ACCCAAGACC GGGACAAACT GCTGAAAGAA CTGGAACAGA TGCAGCAAGA GGATTTGGCT 
CAGAGGAGGC AGGTTGTCGC CCAGATGCCT CAGCAGCTAT TTGAACCGGC CTATAGGCGA 
GAAGAGATAC AGGAGGAGTG GCAGAGGGAA CTGGAGGTTG CTTTTGAAGA CATGTACACT 
GGAGAAAGAA GGGTTAGAGG GGACATGGTT TTGCACCTCA AGCCACAGCC CTTACCAGTT 
CCATCTGCTG AAAGTTTTGA TGATGACCTG GACCTGTCTG TGGATCCAGA GCCCGTGTGT 
GAAACCCAGC CACAAACTGA TGCTGTTGAG GAACCAAGTC ACATTGAGAA AACGGATGAC 
AAAAGCCGCG AGCCACAATC GAGAATTGTT TTTAAGAGAC TGTTGAATAA AATACGAAGC 
CAGCAGGATC AGTGGAGCGC CAAAAGTGAT GTGGAGACGC AGAGCGACAC TGTGGAATCG 
GGATCTCTGC CTAATGAAAT ACATACAATC CATGCAGAAC TCCATGAGGC AAGGAAGCCT 
GCGCCTGAGG GCATTCAGAA CATGAGTGAT AGTACCATTC TAGCTGGTAA TGCAGTTCCT 
CGTCATGCAA CGGAGCCAGG AACACCAACA AAACAGGCCT CTGATTGGAA CAAGCAGGAT 
TTAGAGAGGC AGAAACAGGA GCAGCTGGAT TTGCTGAACA GACTAGAAGA GGAGAAAAAA 
CGCTTAGAAG CCGAATACTT GAGGGTTCAA CTCCAGATGC AAAAATCAAA GCAAGAAGGA 
ATGGAACCTC CAGAGGAGGA GGAGGAGGAA GAGGCAGCTG TAATTCCTCC TGCAGTGACT 
GAACCCAAAA CCATGGAAGA GCCCAAACCT GCGGAACCTC CAGGTTTAAG CCTAAATGTT 
TCCACCGAGA GCCCGCACAT TCAGATAATT CGACAGTACC AGCAACGTCT AATTGAGCAA 
AACAGACAAC ACAAGCAGTC GGTAGAAGAG GCGAGACAAC GTTTGCAAGA ATACCAGCAA 
TTGCTGAAAA AACGCTATTC GCATTTGTCT GCCTCGAGGC TGCAGCCTGC AGCCAAGGAA 
CCCGTTAGTT TCGCTATAAA GCCCGGTCTC GCACAGAAAC CTGCACAGCA CTTGGAAGAC 
ATTGGCGGTG CTTCAGACTC CCATGTTAGA GTAACCTCTT ACCCTGGGGA GAGTCTAGCA 
GGGCACCTTG TATCTCTGGG TAGCTCTTTA ATATCTGCAG TGCCGGTTTC TGGACAAAAC 
TTATTTGCAC CTTCACCAGT AAGCAGAACA GCAGAGTTTG GACAGTCGGG AAGCAGTCCC 
CTCCCTCATT CTACAGAGCA AGATAACTTT ATTCCATACT CTTCTGGGAC TATTCCAACA 
CAACAACAAT ATGGAGGGGC AGAAAGCATG ACCGGAAAAG ACATTGCTGC GAACGGCATT 
AAAAGTCCTA AAAGAGCAAC ACCGTTGGGA AATTTTATTT TGGAAGTGGC AGCTCCCAGG 
CTAGAACAAT ACACTTCTTC GCCCGAAGAA GACTCCATCA GTCCTCCATA TTCTATGTAC 
GATCTGGAAA AGCAGTCCTC CACATCAAGC AAAGGTGATT ATGATACGGC CTCTGATAGT 
TTCTACGCCC TTCCTTCTTC CTTGTCTTTG GGTCTGCCAC AGAATATAGA GAATATAGAG 
CAAGAACCTC ATGTTCAAAA AGTGGATAGT GTTCCATTCG GAGAGTTCTC TAATGTGCAC 
GAGTTCCGCA AGGGGCTGAT CTCTTCAACG GCCAAGATGC AAGCTCAGCA GACTCAGCTT 
AGGGATATGC AGCTGCAGCT GGAGATGCAG AGGGAGTCCC TCTTGTCTAA ACAGAAATCC 
CAAGAAGAAG GTCTGTTACA GAAACAGAAA GAGCTGGAAG ATCAAATGCG CAAGCACCAA 
GAGTCGTTGG AACATTTCCT GGCCAACAAG GAGTCTGCAC AAGGGGCGTT ACCTCCTGAT 
CTCTGTCAGA TTTCAAATAA CGAACGTCTT CGCCTTATGT CTGCGCTGCT GAAAGCACTG 
GGTGACGGTA AACAAGAGAA CCTCAGTCTG GGAAATGGTA GTAGCCTTAG AGCTGCTGGG 
AGAGAACTGA AAGGGAGACC GTCTAAACCT CCGGTTACAA AAACCAAATT GGGTCCATCG 
TTGGAACAAC ATGAGCTCAG TGCTATTCTG GAAGTGGAAA CGCCAAGTGG CAGGCCTTCT 
AGTACTGGGC CGCCGGAACA CAAGAACAGC AGCTCAGGCC GACAGGATGG TGCTGAGGGT 
GGGAACTCGT CTGTGCCCCC TGCTTGCATG CAAGAAAACA GCAGACTGAG GGAAGCCGGT 
AACTATTCTC TCGGAGATCC GGATCTTTCC AGAACATCGA CGAGCAGCAA TGAAAGCAAT 
AGGAGCCAGA CTAAGTTATC TTGGAGACAG ATGTTGTCTC TAGCAGCTGC TTCATCCCAT 
AACTTGACTC TCCCAGATCA ATCCTGGAGC TCTTCAGTGC ACTCAGGCCT TTCTCCTCAT 
CCTGCTGGTA CCAAAACAGC ATTCCACTTT CAGCAACCTG CCCAGGAGAG ATCCGATTGG 
CTGGGGGCAG CTGAGCTGGG AAAAACAGGC ATTGGATACA GTGCTCAAAC GCAGGCCCGG 
GAAGCTTTCA GCGATCACTT GTCAACAACC ACAATCTCTA CGGGAAGTTT TCTGACCAGC 
GAGAAGGCCG ATTCAAGCAT TAGCAACTTA GAGACTATTT CAGACCTTCA CAAATGCAAC 
TTCTCCATTA TCAATGGCGC GGAACACGGC AACTGGAGTA CCTCAGTCAA CGGGGTTCCA 
CAGCCGACTA GAAGGGATGA GTTCCCGAGC CCGGAATATT TGTCGACTGC ACCGGATCAG 
CGCAGCCACA TTCAGCAGAT CATTGATAAA TACACCAGGG ATCTCAGTGC TTCCCTAGAG 
AGAAATCTAA GCTTTCATAC TCCTACTGCT GCTCTAGATG TATCTGGGAA TGACAATCAC 
CTGCCCTCGG GTTTCTATGC CCTGGAACCG AAACCTGACT TCAATGTCTC TACAACTTCC 
CATTCACAAT TAAACAACAC CGGCAGCTCT CAAGAGTCAA AGAACACAAG CCAGAGCTCG 
TTTTACCCAA GCAGCAAAGA GTGGTCTTCA GAACAATCTC ATAGCTTCCA GTATCCAGAC 
AGCAGCCATA GTCCGATGTC TTCCCCTGGC ATAACTAGTG CGCAGCACGT GGATGTGGAA 
ATCACCCCTC ATGTTCGTGG GGATCAAACT CTAGGTTCCT CTGGATCGTT CCACCCTCTG 
CACCCAGAAT GCACCCTTAA TGAGCCACCG CTGAGCAGCC CAGGTCTCTG CGGATGCAGC 
AGTGCAGTTG ATGATCTGGG AAATGGAAGC ATGAAGGCAT CTAAACAACA TGCCTTACAA 
TCATCCAGTG TTGACTCTGG CAATTGGAAC ACAAACAGCA GCCCTGGTGC CTTTCAGCCT 
TCCGTGGAAC CTCTCCGGGC CGATAGCCAC ACCTTGCTGG AGGATCTGGG CTCTTTTCAT 
GAGCTGATGG CCACAGAAAC GACGGCTAAT GAATCTGAGC TGTCTGAGCA CCCACTCACA 
GCTGTCGGCA AAACCAAACA TGGCGACTCT CAGCAGCTTC ATGAATTGCC TGTATTGTAC 
GAAGCACTCG AACCGGCAGA AATAGAGGCA TCTGTAGGGG ACTATCCAGG CCTGGGCTCC 
GTGCCACAGC ATTCCAACGG TGATGAAGGG GCAGAAGTGG AGTCTTCATC TCTGACCTTG 
AAAAGCGACA CACTTACTGA GGCAGAATCC AGCGTACACC CAGATAGTTC ATCTGCCATC 
AGCTCTTCTG CATTCTCTTT GGGCAGCTTT TCTCTTCTCA GGGACACACA CAATGCCCGA 
GGCATCATGG AAGAACCTGA GTTATCGCTA GTGAGTTTCC CTGACAGCAG CTTTGCTGGT 
TCAGAGCACG TTACCACCTT GACTAGCATG TCGGATCACC ACGTGGACCC AAGCTTTGCA 
GAGGGTTGCT TTCATCCCCT ACCAGCTGAA GTGGACCACT CCATGTTTAA CATGCCAGAC 
TGGTCAGCAG CGGGACCAAG CTTTACTGAC CAATCTACAC CACAGCACTT TGCAGCACTT 
GATTGGACAT CCCCTGTGAG CCTGCAGGAA GCCTTATTAA AGAAGAAAAA ACATTTTATC 
GAAAGCTCTT CAAAACGAGT TGAACAAATC AAGCAGAAAG CTGCGGCAGT CGGAAACCCT 
GTATTTAACT CCACGCCGAA TGTTGGAGTC AGCGCGCAGG AGGAGGCGTC TGCCTGTGAC 
GAAGCTTCAG CAAGTGACAG CAGCACCCTG AAGAAGGTTG TAGAAGTTCG TGTGTGTACC 
CCGGAGGAGC GCAAGCTGTC GGAGATAGAG ATGCACCAGA GGACTATAAG GTTGTACAAC 
CAGCTCGACG AAGTTAAAAC CAAAAAAGAG GAGAAGATGA GAAAGGAGTC CTATGCCAGA 
AACAGAGAGA AGGCTAACGA GTTTAAGAAG AAAACTCTGG AAAAGCTGAG ATCCAGGAGG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cep295 ( XM_031895779.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751639.1 Xenopus tropicalis centrosomal protein 295 (cep295), transcript variant X5, mRNA.

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CloneID	OXa51207
Clone ID Related Accession (Same CDS sequence)	XM_031895779.1
Accession Version	XM_031895779.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5163bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	centrosomal protein of 295 kDa isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACGCA AAGTTGCAAA TACCGGCGTC ATAAGACTTA GCCCCAATGA AGAAGCCTTG 
CTGCTAAAGC AGGAACGTGA AAGAAGGCGC AAACTCCGCT TACAACAGGT TAGAGAACAG 
GAGAAGTTCA TTGCCCTGCA AATCCGTCAC AATGTTAAAG AAAGGAGGGA ACAGGAGCTT 
CAGAATTTGG CAGAGGAGCT GCGCTTGGAG TGGCAGGCAT CGCAGGCTGA GAAGCTCCGA 
GCCCTGGAGA AGCTTTATGT GTCCAGTCTA AGTGCGATTG GAGAGGGGCA CAGGCAAGCC 
AAGGAAAATG AACCGGACCT TCAAGCCGTT GAGAGGCAAG CGGCAGCCAA TAGACAGAAA 
GCAGAGAAGC GGCACAGAGA AGCTCTCAGG GAATTAAAGC AGCACAAAGA AAAACAATTG 
AGAGAGCAAA CCTGGCTTTT GAAAGCCCGC AGGAAAGCGC TTATTACTGA GAAGGAGCGT 
GCTGCAAAGA TAGCAAGCCT TCCTCCCCCA CCCCCAGACC CTCTAGAGAA TATTGAGGTT 
TTGCGAAGGT TCCCAACCGT GAAGCTGTGC AATGTTGATG GCTTCTCTGT ATCTCATTAT 
CATCTGCCTG AACCATACGT AGACCGAGAG ACGGATACGG AACAGCCAGA TGCTCGGGTT 
GCCGCTGAAG AGGAAGCAAA GCGTTTGGAT GCCTTACAGA AAGAGGAAGA GAGAGAAAGG 
AGGGAAAGGA TGGAGAAGGC TCACTTACGG GGATCCCATG CCTTGAAAAA GGTTCTTTTA 
ACCCAAGACC GGGACAAACT GCTGAAAGAA CTGGAACAGA TGCAGCAAGA GGATTTGGCT 
CAGAGGAGGC AGGTTGTCGC CCAGATGCCT CAGCAGCTAT TTGAACCGGC CTATAGGCGA 
GAAGAGATAC AGGAGGAGTG GCAGAGGGAA CTGGAGGTTG CTTTTGAAGA CATGTACACT 
GGAGAAAGAA GGGTTAGAGG GGACATGGTT TTGCACCTCA AGCCACAGCC CTTACCAGTT 
CCATCTGCTG AAAGTTTTGA TGATGACCTG GACCTGTCTG TGGATCCAGA GCCCGTGTGT 
GAAACCCAGC CACAAACTGA TGCTGTTGAG GAACCAAGTC ACATTGAGAA AACGGATGAC 
AAAAGCCGCG AGCCACAATC GAGAATTGTT TTTAAGAGAC TGTTGAATAA AATACGAAGC 
CAGCAGGATC AGTGGAGCGC CAAAAGTGAT GTGGAGACGC AGAGCGACAC TGTGGAATCG 
GGATCTCTGC CTAATGAAAT ACATACAATC CATGCAGAAC TCCATGAGGC AAGGAAGCCT 
GCGCCTGAGG GCATTCAGAA CATGAGTGAT AGTACCATTC TAGCTGGTAA TGCAGTTCCT 
CGTCATGCAA CGGAGCCAGG AACACCAACA AAACAGGCCT CTGATTGGAA CAAGCAGGAT 
TTAGAGAGGC AGAAACAGGA GCAGCTGGAT TTGCTGAACA GACTAGAAGA GGAGAAAAAA 
CGCTTAGAAG CCGAATACTT GAGGGTTCAA CTCCAGATGC AAAAATCAAA GCAAGAAGGA 
ATGGAACCTC CAGAGGAGGA GGAGGAGGAA GAGGCAGCTG TAATTCCTCC TGCAGTGACT 
GAACCCAAAA CCATGGAAGA GCCCAAACCT GCGCAGGAAC CTCCAGGTTT AAGCCTAAAT 
GTTTCCACCG AGAGCCCGCA CATTCAGATA ATTCGACAGT ACCAGCAACG TCTAATTGAG 
CAAAACAGAC AACACAAGCA GTCGGTAGAA GAGGCGAGAC AACGTTTGCA AGAATACCAG 
CAATTGCTGA AAAAACGCTA TTCGCATTTG TCTGCCTCGA GGCTGCAGCC TGCAGCCAAG 
GAACCCGTTA GTTTCGCTAT AAAGCCCGGT CTCGCACAGA AACCTGCACA GCACTTGGAA 
GACATTGGCG GTGCTTCAGA CTCCCATGTT AGAGTAACCT CTTACCCTGG GGAGAGTCTA 
GCAGGGCACC TTGTATCTCT GGGTAGCTCT TTAATATCTG CAGTGCCGGT TTCTGGACAA 
AACTTATTTG CACCTTCACC AGTAAGCAGA ACAGCAGAGT TTGGACAGTC GGGAAGCAGT 
CCCCTCCCTC ATTCTACAGA GCAAGATAAC TTTATTCCAT ACTCTTCTGG GACTATTCCA 
ACACAACAAC AATATGGAGG GGCAGAAAGC ATGACCGGAA AAGACATTGC TGCGAACGGC 
ATTAAAAGTC CTAAAAGAGC AACACCGTTG GGAAATTTTA TTTTGGAAGT GGCAGCTCCC 
AGGCTAGAAC AATACACTTC TTCGCCCGAA GAAGACTCCA TCAGTCCTCC ATATTCTATG 
TACGATCTGG AAAAGCAGTC CTCCACATCA AGCAAAGGTG ATTATGATAC GGCCTCTGAT 
AGTTTCTACG CCCTTCCTTC TTCCTTGTCT TTGGGTCTGC CACAGAATAT AGAGAATATA 
GAGCAAGAAC CTCATGTTCA AAAAGTGGAT AGTGTTCCAT TCGGAGAGTT CTCTAATGTG 
CACGAGTTCC GCAAGGGGCT GATCTCTTCA ACGGCCAAGA TGCAAGCTCA GCAGACTCAG 
CTTAGGGATA TGCAGCTGCA GCTGGAGATG CAGAGGGAGT CCCTCTTGTC TAAACAGAAA 
TCCCAAGAAG AAGGTCTGTT ACAGAAACAG AAAGAGCTGG AAGATCAAAT GCGCAAGCAC 
CAAGAGTCGT TGGAACATTT CCTGGCCAAC AAGGAGTCTG CACAAGGGGC GTTACCTCCT 
GATCTCTGTC AGATTTCAAA TAACGAACGT CTTCGCCTTA TGTCTGCGCT GCTGAAAGCA 
CTGGGTGACG GTAAACAAGA GAACCTCAGT CTGGGAAATG GTAGTAGCCT TAGAGCTGCT 
GGGAGAGAAC TGAAAGGGAG ACCGTCTAAA CCTCCGGTTA CAAAAACCAA ATTGGGTCCA 
TCGTTGGAAC AACATGAGCT CAGTGCTATT CTGGAAGTGG AAACGCCAAG TGGCAGGCCT 
TCTAGTACTG GGCCGCCGGA ACACAAGAAC AGCAGCTCAG GCCGACAGGA TGGTGCTGAG 
GGTGGGAACT CGTCTGTGCC CCCTGCTTGC ATGCAAGAAA ACAGCAGACT GAGGGAAGCC 
GGTAACTATT CTCTCGGAGA TCCGGATCTT TCCAGAACAT CGACGAGCAG CAATGAAAGC 
AATAGGAGCC AGACTAAGTT ATCTTGGAGA CAGATGTTGT CTCTAGCAGC TGCTTCATCC 
CATAACTTGA CTCTCCCAGA TCAATCCTGG AGCTCTTCAG TGCACTCAGG CCTTTCTCCT 
CATCCTGCTG GTACCAAAAC AGCATTCCAC TTTCAGCAAC CTGCCCAGGA GAGATCCGAT 
TGGCTGGGGG CAGCTGAGCT GGGAAAAACA GGCATTGGAT ACAGTGCTCA AACGCAGGCC 
CGGGAAGCTT TCAGCGATCA CTTGTCAACA ACCACAATCT CTACGGGAAG TTTTCTGACC 
AGCGAGAAGG CCGATTCAAG CATTAGCAAC TTAGAGACTA TTTCAGACCT TCACAAATGC 
AACTTCTCCA TTATCAATGG CGCGGAACAC GGCAACTGGA GTACCTCAGT CAACGGGGTT 
CCACAGCCGA CTAGAAGGGA TGAGTTCCCG AGCCCGGAAT ATTTGTCGAC TGCACCGGAT 
CAGCGCAGCC ACATTCAGCA GATCATTGAT AAATACACCA GGGATCTCAG TGCTTCCCTA 
GAGAGAAATC TAAGCTTTCA TACTCCTACT GCTGCTCTAG ATGTATCTGG GAATGACAAT 
CACCTGCCCT CGGGTTTCTA TGCCCTGGAA CCGAAACCTG ACTTCAATGT CTCTACAACT 
TCCCATTCAC AATTAAACAA CACCGGCAGC TCTCAAGAGT CAAAGAACAC AAGCCAGAGC 
TCGTTTTACC CAAGCAGCAA AGAGTGGTCT TCAGAACAAT CTCATAGCTT CCAGTATCCA 
GACAGCAGCC ATAGTCCGAT GTCTTCCCCT GGCATAACTA GTGCGCAGCA CGTGGATGTG 
GAAATCACCC CTCATGTTCG TGGGGATCAA ACTCTAGGTT CCTCTGGATC GTTCCACCCT 
CTGCACCCAG AATGCACCCT TAATGAGCCA CCGCTGAGCA GCCCAGGTCT CTGCGGATGC 
AGCAGTGCAG TTGATGATCT GGGAAATGGA AGCATGAAGG CATCTAAACA ACATGCCTTA 
CAATCATCCA GTGTTGACTC TGGCAATTGG AACACAAACA GCAGCCCTGG TGCCTTTCAG 
CCTTCCGTGG AACCTCTCCG GGCCGATAGC CACACCTTGC TGGAGGATCT GGGCTCTTTT 
CATGAGCTGA TGGCCACAGA AACGACGGCT AATGAATCTG AGCTGTCTGA GCACCCACTC 
ACAGCTGTCG GCAAAACCAA ACATGGCGAC TCTCAGCAGC TTCATGAATT GCCTGTATTG 
TACGAAGCAC TCGAACCGGC AGAAATAGAG GCATCTGTAG GGGACTATCC AGGCCTGGGC 
TCCGTGCCAC AGCATTCCAA CGGTGATGAA GGGGCAGAAG TGGAGTCTTC ATCTCTGACC 
TTGAAAAGCG ACACACTTAC TGAGGCAGAA TCCAGCGTAC ACCCAGATAG TTCATCTGCC 
ATCAGCTCTT CTGCATTCTC TTTGGGCAGC TTTTCTCTTC TCAGGGACAC ACACAATGCC 
CGAGGCATCA TGGAAGAACC TGAGTTATCG CTAGTGAGTT TCCCTGACAG CAGCTTTGCT 
GGTTCAGAGC ACGTTACCAC CTTGACTAGC ATGTCGGATC ACCACGTGGA CCCAAGCTTT 
GCAGAGGGTT GCTTTCATCC CCTACCAGCT GAAGTGGACC ACTCCATGTT TAACATGCCA 
GACTGGTCAG CAGCGGGACC AAGCTTTACT GACCAATCTA CACCACAGCA CTTTGCAGAA 
GCTTCAGCAA GTGACAGCAG CACCCTGAAG AAGGTTGTAG AAGTTCGTGT GTGTACCCCG 
GAGGAGCGCA AGCTGTCGGA GATAGAGATG CACCAGAGGA CTATAAGGTT GTACAACCAG 
CTCGACGAAG TTAAAACCAA AAAAGAGGAG AAGATGAGAA AGGAGTCCTA TGCCAGAAAC 
AGAGAGAAGG CTAACGAGTT TAAGAAGAAA ACTCTGGAAA AGCTGAGATC CAGGAGGAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751639.1
CDS	140..5302
Translation	MKRKVANTGVIRLSPNEEALLLKQERERRRKLRLQQVREQEKFIALQIRHNVKERREQELQNLAEELRLEWQASQAEKLRALEKLYVSSLSAIGEGHRQAKENEPDLQAVERQAAANRQKAEKRHREALRELKQHKEKQLREQTWLLKARRKALITEKERAAKIASLPPPPPDPLENIEVLRRFPTVKLCNVDGFSVSHYHLPEPYVDRETDTEQPDARVAAEEEAKRLDALQKEEERERRERMEKAHLRGSHALKKVLLTQDRDKLLKELEQMQQEDLAQRRQVVAQMPQQLFEPAYRREEIQEEWQRELEVAFEDMYTGERRVRGDMVLHLKPQPLPVPSAESFDDDLDLSVDPEPVCETQPQTDAVEEPSHIEKTDDKSREPQSRIVFKRLLNKIRSQQDQWSAKSDVETQSDTVESGSLPNEIHTIHAELHEARKPAPEGIQNMSDSTILAGNAVPRHATEPGTPTKQASDWNKQDLERQKQEQLDLLNRLEEEKKRLEAEYLRVQLQMQKSKQEGMEPPEEEEEEEAAVIPPAVTEPKTMEEPKPAQEPPGLSLNVSTESPHIQIIRQYQQRLIEQNRQHKQSVEEARQRLQEYQQLLKKRYSHLSASRLQPAAKEPVSFAIKPGLAQKPAQHLEDIGGASDSHVRVTSYPGESLAGHLVSLGSSLISAVPVSGQNLFAPSPVSRTAEFGQSGSSPLPHSTEQDNFIPYSSGTIPTQQQYGGAESMTGKDIAANGIKSPKRATPLGNFILEVAAPRLEQYTSSPEEDSISPPYSMYDLEKQSSTSSKGDYDTASDSFYALPSSLSLGLPQNIENIEQEPHVQKVDSVPFGEFSNVHEFRKGLISSTAKMQAQQTQLRDMQLQLEMQRESLLSKQKSQEEGLLQKQKELEDQMRKHQESLEHFLANKESAQGALPPDLCQISNNERLRLMSALLKALGDGKQENLSLGNGSSLRAAGRELKGRPSKPPVTKTKLGPSLEQHELSAILEVETPSGRPSSTGPPEHKNSSSGRQDGAEGGNSSVPPACMQENSRLREAGNYSLGDPDLSRTSTSSNESNRSQTKLSWRQMLSLAAASSHNLTLPDQSWSSSVHSGLSPHPAGTKTAFHFQQPAQERSDWLGAAELGKTGIGYSAQTQAREAFSDHLSTTTISTGSFLTSEKADSSISNLETISDLHKCNFSIINGAEHGNWSTSVNGVPQPTRRDEFPSPEYLSTAPDQRSHIQQIIDKYTRDLSASLERNLSFHTPTAALDVSGNDNHLPSGFYALEPKPDFNVSTTSHSQLNNTGSSQESKNTSQSSFYPSSKEWSSEQSHSFQYPDSSHSPMSSPGITSAQHVDVEITPHVRGDQTLGSSGSFHPLHPECTLNEPPLSSPGLCGCSSAVDDLGNGSMKASKQHALQSSSVDSGNWNTNSSPGAFQPSVEPLRADSHTLLEDLGSFHELMATETTANESELSEHPLTAVGKTKHGDSQQLHELPVLYEALEPAEIEASVGDYPGLGSVPQHSNGDEGAEVESSSLTLKSDTLTEAESSVHPDSSSAISSSAFSLGSFSLLRDTHNARGIMEEPELSLVSFPDSSFAGSEHVTTLTSMSDHHVDPSFAEGCFHPLPAEVDHSMFNMPDWSAAGPSFTDQSTPQHFAEASASDSSTLKKVVEVRVCTPEERKLSEIEMHQRTIRLYNQLDEVKTKKEEKMRKESYARNREKANEFKKKTLEKLRSRRK

Target ORF information:

RefSeq Version	XM_031895779.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis centrosomal protein 295 (cep295), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895779.1

ORF Insert Sequence:

ATGAAACGCA AAGTTGCAAA TACCGGCGTC ATAAGACTTA GCCCCAATGA AGAAGCCTTG 
CTGCTAAAGC AGGAACGTGA AAGAAGGCGC AAACTCCGCT TACAACAGGT TAGAGAACAG 
GAGAAGTTCA TTGCCCTGCA AATCCGTCAC AATGTTAAAG AAAGGAGGGA ACAGGAGCTT 
CAGAATTTGG CAGAGGAGCT GCGCTTGGAG TGGCAGGCAT CGCAGGCTGA GAAGCTCCGA 
GCCCTGGAGA AGCTTTATGT GTCCAGTCTA AGTGCGATTG GAGAGGGGCA CAGGCAAGCC 
AAGGAAAATG AACCGGACCT TCAAGCCGTT GAGAGGCAAG CGGCAGCCAA TAGACAGAAA 
GCAGAGAAGC GGCACAGAGA AGCTCTCAGG GAATTAAAGC AGCACAAAGA AAAACAATTG 
AGAGAGCAAA CCTGGCTTTT GAAAGCCCGC AGGAAAGCGC TTATTACTGA GAAGGAGCGT 
GCTGCAAAGA TAGCAAGCCT TCCTCCCCCA CCCCCAGACC CTCTAGAGAA TATTGAGGTT 
TTGCGAAGGT TCCCAACCGT GAAGCTGTGC AATGTTGATG GCTTCTCTGT ATCTCATTAT 
CATCTGCCTG AACCATACGT AGACCGAGAG ACGGATACGG AACAGCCAGA TGCTCGGGTT 
GCCGCTGAAG AGGAAGCAAA GCGTTTGGAT GCCTTACAGA AAGAGGAAGA GAGAGAAAGG 
AGGGAAAGGA TGGAGAAGGC TCACTTACGG GGATCCCATG CCTTGAAAAA GGTTCTTTTA 
ACCCAAGACC GGGACAAACT GCTGAAAGAA CTGGAACAGA TGCAGCAAGA GGATTTGGCT 
CAGAGGAGGC AGGTTGTCGC CCAGATGCCT CAGCAGCTAT TTGAACCGGC CTATAGGCGA 
GAAGAGATAC AGGAGGAGTG GCAGAGGGAA CTGGAGGTTG CTTTTGAAGA CATGTACACT 
GGAGAAAGAA GGGTTAGAGG GGACATGGTT TTGCACCTCA AGCCACAGCC CTTACCAGTT 
CCATCTGCTG AAAGTTTTGA TGATGACCTG GACCTGTCTG TGGATCCAGA GCCCGTGTGT 
GAAACCCAGC CACAAACTGA TGCTGTTGAG GAACCAAGTC ACATTGAGAA AACGGATGAC 
AAAAGCCGCG AGCCACAATC GAGAATTGTT TTTAAGAGAC TGTTGAATAA AATACGAAGC 
CAGCAGGATC AGTGGAGCGC CAAAAGTGAT GTGGAGACGC AGAGCGACAC TGTGGAATCG 
GGATCTCTGC CTAATGAAAT ACATACAATC CATGCAGAAC TCCATGAGGC AAGGAAGCCT 
GCGCCTGAGG GCATTCAGAA CATGAGTGAT AGTACCATTC TAGCTGGTAA TGCAGTTCCT 
CGTCATGCAA CGGAGCCAGG AACACCAACA AAACAGGCCT CTGATTGGAA CAAGCAGGAT 
TTAGAGAGGC AGAAACAGGA GCAGCTGGAT TTGCTGAACA GACTAGAAGA GGAGAAAAAA 
CGCTTAGAAG CCGAATACTT GAGGGTTCAA CTCCAGATGC AAAAATCAAA GCAAGAAGGA 
ATGGAACCTC CAGAGGAGGA GGAGGAGGAA GAGGCAGCTG TAATTCCTCC TGCAGTGACT 
GAACCCAAAA CCATGGAAGA GCCCAAACCT GCGCAGGAAC CTCCAGGTTT AAGCCTAAAT 
GTTTCCACCG AGAGCCCGCA CATTCAGATA ATTCGACAGT ACCAGCAACG TCTAATTGAG 
CAAAACAGAC AACACAAGCA GTCGGTAGAA GAGGCGAGAC AACGTTTGCA AGAATACCAG 
CAATTGCTGA AAAAACGCTA TTCGCATTTG TCTGCCTCGA GGCTGCAGCC TGCAGCCAAG 
GAACCCGTTA GTTTCGCTAT AAAGCCCGGT CTCGCACAGA AACCTGCACA GCACTTGGAA 
GACATTGGCG GTGCTTCAGA CTCCCATGTT AGAGTAACCT CTTACCCTGG GGAGAGTCTA 
GCAGGGCACC TTGTATCTCT GGGTAGCTCT TTAATATCTG CAGTGCCGGT TTCTGGACAA 
AACTTATTTG CACCTTCACC AGTAAGCAGA ACAGCAGAGT TTGGACAGTC GGGAAGCAGT 
CCCCTCCCTC ATTCTACAGA GCAAGATAAC TTTATTCCAT ACTCTTCTGG GACTATTCCA 
ACACAACAAC AATATGGAGG GGCAGAAAGC ATGACCGGAA AAGACATTGC TGCGAACGGC 
ATTAAAAGTC CTAAAAGAGC AACACCGTTG GGAAATTTTA TTTTGGAAGT GGCAGCTCCC 
AGGCTAGAAC AATACACTTC TTCGCCCGAA GAAGACTCCA TCAGTCCTCC ATATTCTATG 
TACGATCTGG AAAAGCAGTC CTCCACATCA AGCAAAGGTG ATTATGATAC GGCCTCTGAT 
AGTTTCTACG CCCTTCCTTC TTCCTTGTCT TTGGGTCTGC CACAGAATAT AGAGAATATA 
GAGCAAGAAC CTCATGTTCA AAAAGTGGAT AGTGTTCCAT TCGGAGAGTT CTCTAATGTG 
CACGAGTTCC GCAAGGGGCT GATCTCTTCA ACGGCCAAGA TGCAAGCTCA GCAGACTCAG 
CTTAGGGATA TGCAGCTGCA GCTGGAGATG CAGAGGGAGT CCCTCTTGTC TAAACAGAAA 
TCCCAAGAAG AAGGTCTGTT ACAGAAACAG AAAGAGCTGG AAGATCAAAT GCGCAAGCAC 
CAAGAGTCGT TGGAACATTT CCTGGCCAAC AAGGAGTCTG CACAAGGGGC GTTACCTCCT 
GATCTCTGTC AGATTTCAAA TAACGAACGT CTTCGCCTTA TGTCTGCGCT GCTGAAAGCA 
CTGGGTGACG GTAAACAAGA GAACCTCAGT CTGGGAAATG GTAGTAGCCT TAGAGCTGCT 
GGGAGAGAAC TGAAAGGGAG ACCGTCTAAA CCTCCGGTTA CAAAAACCAA ATTGGGTCCA 
TCGTTGGAAC AACATGAGCT CAGTGCTATT CTGGAAGTGG AAACGCCAAG TGGCAGGCCT 
TCTAGTACTG GGCCGCCGGA ACACAAGAAC AGCAGCTCAG GCCGACAGGA TGGTGCTGAG 
GGTGGGAACT CGTCTGTGCC CCCTGCTTGC ATGCAAGAAA ACAGCAGACT GAGGGAAGCC 
GGTAACTATT CTCTCGGAGA TCCGGATCTT TCCAGAACAT CGACGAGCAG CAATGAAAGC 
AATAGGAGCC AGACTAAGTT ATCTTGGAGA CAGATGTTGT CTCTAGCAGC TGCTTCATCC 
CATAACTTGA CTCTCCCAGA TCAATCCTGG AGCTCTTCAG TGCACTCAGG CCTTTCTCCT 
CATCCTGCTG GTACCAAAAC AGCATTCCAC TTTCAGCAAC CTGCCCAGGA GAGATCCGAT 
TGGCTGGGGG CAGCTGAGCT GGGAAAAACA GGCATTGGAT ACAGTGCTCA AACGCAGGCC 
CGGGAAGCTT TCAGCGATCA CTTGTCAACA ACCACAATCT CTACGGGAAG TTTTCTGACC 
AGCGAGAAGG CCGATTCAAG CATTAGCAAC TTAGAGACTA TTTCAGACCT TCACAAATGC 
AACTTCTCCA TTATCAATGG CGCGGAACAC GGCAACTGGA GTACCTCAGT CAACGGGGTT 
CCACAGCCGA CTAGAAGGGA TGAGTTCCCG AGCCCGGAAT ATTTGTCGAC TGCACCGGAT 
CAGCGCAGCC ACATTCAGCA GATCATTGAT AAATACACCA GGGATCTCAG TGCTTCCCTA 
GAGAGAAATC TAAGCTTTCA TACTCCTACT GCTGCTCTAG ATGTATCTGG GAATGACAAT 
CACCTGCCCT CGGGTTTCTA TGCCCTGGAA CCGAAACCTG ACTTCAATGT CTCTACAACT 
TCCCATTCAC AATTAAACAA CACCGGCAGC TCTCAAGAGT CAAAGAACAC AAGCCAGAGC 
TCGTTTTACC CAAGCAGCAA AGAGTGGTCT TCAGAACAAT CTCATAGCTT CCAGTATCCA 
GACAGCAGCC ATAGTCCGAT GTCTTCCCCT GGCATAACTA GTGCGCAGCA CGTGGATGTG 
GAAATCACCC CTCATGTTCG TGGGGATCAA ACTCTAGGTT CCTCTGGATC GTTCCACCCT 
CTGCACCCAG AATGCACCCT TAATGAGCCA CCGCTGAGCA GCCCAGGTCT CTGCGGATGC 
AGCAGTGCAG TTGATGATCT GGGAAATGGA AGCATGAAGG CATCTAAACA ACATGCCTTA 
CAATCATCCA GTGTTGACTC TGGCAATTGG AACACAAACA GCAGCCCTGG TGCCTTTCAG 
CCTTCCGTGG AACCTCTCCG GGCCGATAGC CACACCTTGC TGGAGGATCT GGGCTCTTTT 
CATGAGCTGA TGGCCACAGA AACGACGGCT AATGAATCTG AGCTGTCTGA GCACCCACTC 
ACAGCTGTCG GCAAAACCAA ACATGGCGAC TCTCAGCAGC TTCATGAATT GCCTGTATTG 
TACGAAGCAC TCGAACCGGC AGAAATAGAG GCATCTGTAG GGGACTATCC AGGCCTGGGC 
TCCGTGCCAC AGCATTCCAA CGGTGATGAA GGGGCAGAAG TGGAGTCTTC ATCTCTGACC 
TTGAAAAGCG ACACACTTAC TGAGGCAGAA TCCAGCGTAC ACCCAGATAG TTCATCTGCC 
ATCAGCTCTT CTGCATTCTC TTTGGGCAGC TTTTCTCTTC TCAGGGACAC ACACAATGCC 
CGAGGCATCA TGGAAGAACC TGAGTTATCG CTAGTGAGTT TCCCTGACAG CAGCTTTGCT 
GGTTCAGAGC ACGTTACCAC CTTGACTAGC ATGTCGGATC ACCACGTGGA CCCAAGCTTT 
GCAGAGGGTT GCTTTCATCC CCTACCAGCT GAAGTGGACC ACTCCATGTT TAACATGCCA 
GACTGGTCAG CAGCGGGACC AAGCTTTACT GACCAATCTA CACCACAGCA CTTTGCAGAA 
GCTTCAGCAA GTGACAGCAG CACCCTGAAG AAGGTTGTAG AAGTTCGTGT GTGTACCCCG 
GAGGAGCGCA AGCTGTCGGA GATAGAGATG CACCAGAGGA CTATAAGGTT GTACAACCAG 
CTCGACGAAG TTAAAACCAA AAAAGAGGAG AAGATGAGAA AGGAGTCCTA TGCCAGAAAC 
AGAGAGAAGG CTAACGAGTT TAAGAAGAAA ACTCTGGAAA AGCTGAGATC CAGGAGGAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cep295 ( NM_001199923.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001186852.1 Xenopus tropicalis centrosomal protein 295 (cep295), mRNA.

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CloneID	OXa24275
Clone ID Related Accession (Same CDS sequence)	NM_001199923.1
Accession Version	NM_001199923.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5349bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	centrosomal protein of 295 kDa
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU024935.1, AL850279.2, CX405223.2, CX915430.1, CX931635.2, AL896340.2, CX440535.2, DT404564.1 and AL881139.2. On or before Mar 27, 2011 this sequence version replaced XM_002940144.1, XM_002940145.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

ATGAAACGCA AAGTTGCAAA TACCGGCGTC ATAAGACTTA GCCCCAATGA AGAAGCCTTG 
CTGCTAAAGC AGGAACGTGA AAGAAGGCGC AAACTCCGCT TACAACAGGT TAGAGAACAG 
GAGAAGTTCA TTGCCCTGCA AATCCGTCAC AATGTTAAAG AAAGGAGGGA ACAGGAGCTT 
CAGAATTTGG CAGAGGAGCT GCGCTTGGAG TGGCAGGCAT CGCAGGCTGA GAAGCTCCGA 
GCCCTGGAGA AGCTTTATGT GTCCAGTCTA AGTGCGATTG GAGAGGGGCA CAGGCAAGCC 
AAGGAAAATG AACCGGACCT TCAAGCCGTT GAGAGGCAAG CGGCAGCCAA TAGACAGAAA 
GCAGAGAAGC GGCACAGAGA AGCTCTCAGG GAATTAAAGC AGCACAAAGA AAAACAATTG 
AGAGAGCAAA CCTGGCTTTT GAAAGCCCGC AGGAAAGCGC TTATTACTGA GAAGGAGCGT 
GCTGCAAAGA TAGCAAGCCT TCCTCCCCCA CCCCCAGACC CTCTAGAGAA TATTGAGGTT 
TTGCGAAGGT TCCCAACCGT GAAGCTGTGC AATGTTGATG GCTTCTCTGT ATCTCATTAT 
CATCTGCCTG AACCATACGT AGACCGAGAG ACGGATACGG AACAGCCAGA TGCTCGGGTT 
GCCGCTGAAG AGGAAGCAAA GCGTTTGGAT GCCTTACAGA AAGAGGAAGA GAGAGAAAGG 
AGGGAAAGGA TGGAGAAGGC TCACTTACGG GGATCCCATG CCTTGAAAAA GGTTCTTTTA 
ACCCAAGACC GGGACAAACT GCTGAAAGAA CTGGAACAGA TGCAGCAAGA GGATTTGGCT 
CAGAGGAGGC AGGTTGTCGC CCAGATGCCT CAGCAGCTAT TTGAACCGGC CTATAGGCGA 
GAAGAGATAC AGGAGGAGTG GCAGAGGGAA CTGGAGGTTG CTTTTGAAGA CATGTACACT 
GGAGAAAGAA GGGTTAGAGG GGACATGGTT TTGCACCTCA AGCCACAGCC CTTACCAGTT 
CCATCTGCTG AAAGTTTTGA TGATGACCTG GACCTGTCTG TGGATCCAGA GCCCGTGTGT 
GAAACCCAGC CACAAACTGA TGCTGTTGAG GAACCAAGTC ACATTGAGAA AACGGATGAC 
AAAAGCCGCG AGCCACAATC GAGAATTGTT TTTAAGAGAC TATTGAATAA AATACGAAGC 
CAGCAGGATC AGTGGAGCGC CAAAAGTGAT GTGGAGACGC AGAGCGACAC TGTGGAATCG 
GGATCTCTGC CTAATGAAAT ACATACAATC CATGCAGAAC TCCATGAGGC AAGGAAGCCT 
GCGCCTGAGG GCATTCAGAA CATGAGTGAT AGTACCATTC TAGCTGGTAA TGCAGTTCCT 
CGTCATGCAA CGGAGCCAGG AACACCAACA AAACAGGCCT CTGATTGGAA CAAGCAGGAT 
TTAGAGAGGC AGAAACAGGA GCAGCTGGAT TTGCTGAACA GACTAGAAGA GGAGAAAAAA 
CGCTTAGAAG CCGAATACTT GAGGGTTCAA CTCCAGATGC AAAAATCAAA GCAAGAAGGA 
ATGGAACCTC CAGAGGAGGA GGAGGAGGAA GAGGCAGCTG TAATTCCTCC TGCAGTGACT 
GAATCCAAAA CCATGGAAGA GCCCAAACCT GCGCAGGAAC CTCCAGGTTT AAGCCTAAAT 
GTTTCCACCG AGAGCCCGCA CATTCAGATA ATTCGACAGT ACCAGCAACG TCTAATTGAG 
CAAAACAGAC AACACAAGCA GTCGGTAGAA GAGGCGAGAC AACGTTTGCA AGAATACCAG 
CAATTGCTGA AAAAACGCTA TTCGCATTTG TCTGCCTCGA GGCTGCAGCC TGCAGCCAAG 
GAACCCGTTA GTTTCGCTAT AAAGCCCGGT CTCGCACAGA AACCTGCACA GCACTTGGAA 
GACATTGGCG GTGCTTCAGA CTCCCATGTT AGAGTAACCT CTTACCCTGG GGAGAGTCTA 
GCAGGGCACC TTGTATCTCT GGGTAGCTCT TTAATATCTG CAGTGCCGGT TTCTGGACAA 
AACTTATTTG CACCTTCACC AGTAAGCAGA ACAGCAGAGT TTGGACAGTC GGGAAGCAGT 
CCCCTCCCTC ATTCTACAGA GCAAGATAAC TTTATTCCAT ACTCTTCTGG GACTATTCCA 
ACACAACAAC AATATGGAGG GGCAGAAAGC ATGACCGGAA AAGACATTGC TGCGAACGGC 
ATTAAAAGTC CTAAAAGAGC AACACCGTTG GGAAATTTTA TTTTGGAAGT GGCAGCTCCC 
AGGCTAGAAC AATACACTTC TTCGCCCGAA GAAGACTCCA TCAGTCCTCC ATATTCTATG 
TACGATCTGG AAAAGCAGTC CTCCACATCA AGCAAAGGTG ATTATGATAC GGCCTCTGAT 
AGTTTCTACG CCCTTCCTTC TTCCTTGTCT TTGGGTCTGC CACAGAATAT AGAGAATATA 
GAGCAAGAAC CTCATGTTCA AAAAGTGGAT AGTGTTCCAT TCGGAGAGTT CTCTAATGTG 
CACGAGTTCC GCAAGGGGCT GATCTCTTCA ACGGCCAAGA TGCAAGCTCA GCAGACTCAG 
CTTAGGGATA TGCAGCTGCA GCTGGAGATG CAGAGGGAGT CCCTCTTGTC TAAACAGAAA 
TCCCAAGAAG AAGGTCTGTT ACAGAAACAG AAAGAGCTGG AAGATCAAAT GCGCAAGCAC 
CAAGAGTCGT TGGAACATTT CCTGGCCAAC AAGGAGTCTG CACAAGGGGC GTTACCTCCT 
GATCTCTGTC AGATTTCAAA TAACGAACGT CTTCGCCTTA TGTCTGCGCT GCTGAAAGCA 
CTGGGTGACG GTAAACAAGA GAACCTCAGT CTGGGAAATG GTAGTAGCCT TAGAGCTGCT 
GGGAGAGAAC TGAAAGGGAG ACCGTCTAAA CCTCCGGTTA CAAAAACCAA ATTGGGTCCA 
TCGTTGGAAC AACATGAGCT CAGTGCTATT CTGGAAGTGG AAACGCCAAG TGGCAGGCCT 
TCTAGTACTG GGCCGCCGGA ACACAAGAAC AGCAGCTCAG GCCGACAGGA TGGTGCCGAG 
GGTGGGAACT CGTCTGTGCC CCCTGCTTGC ATGCAAGAAA ACAGCAGACT GAGGGAAGCT 
GGTAACTATT CTCTCGGAGA TCCGGATCTT TCCAGAACAT CGACGAGCAG CAATGAAAGC 
AATAGGAGCC AGACTAAGTT ATCTTGGAGA CAGATGTTGT CTCTAGCAGC TGCTTCATCC 
CATAACTTGA CTCTCCCAGA TCAATCCTGG AGCTCTTCAG TGCACTCAGG CCTTTCTCCT 
CATCCTGCTG GTACCAAAAC AGCATTCCAC TTTCAGCAAC CTGCCCAGGA GAGATCCGAT 
TGGCTGGGGG CAGCTGAGCT GGGAAAAACA GGCATTGGAT ACAGTGCTCA AACGCAGGCC 
CGGGACGCTT TCAGCGATCA CTTGTCAACA ACCACAATCT CTACGGGAAG TTTTCTGACC 
AGCGAGAAGG CTGATTCAAG CATTAGCAAC TTAGAGACTA TTTCAGACCT TCACAAATGC 
AACTTCTCCA TTATCAATGG CGCGGAACAC GGCAACTGGA GTACCTCAGT CAACGGGGTT 
CCACAGCCGA CTAGAAGGGA TGAGTTCCCG AGCCCGGAAT ATTTGTCGAC TGCACCGGAT 
CAGCGCAGCC ACATTCAGCA GATCATTGAT AAATACACCA GGGATCTCAG TGCTTCCCTA 
GAGAGAAATC TAAGCTTTCA TACTCCTACT GCTGCTCTAG ATGTATCTGG GAATGACAAT 
CACCTGCCCT CGGGTTTCTA TGCCCTGGAA CCGAAACCTG ACTTCAATGT CTCTACAACT 
TCCCATTCAC AATTAAACAA CACCGGCAGC TCTCAAGAGG CAAAGAACAC AAGCCAGAGC 
TCGTTTTACC CAAGCAGCAA AGAGTGGTCT TCAGAACAAT CTCATAGCTT CCAGTATCCA 
GACAGCAGCC ATAGTCCGAT GTCTTCCCCT GGCATAACTA GTGCGCAGCA CGTGGATGTG 
GAAATCACCC CTCATGTTCG TGGGGATCAA ACTCTAGGTT CCTCTGGATC GTTCCACCCT 
CTGCACCCAG AATGCACCCT TAATGAGCCA CCGCTGAGCA GCCCAGGTCT CTGCGGATGC 
AGCAGTGCAG TTGATGATCT GGGAAATGGA AGCATGAAGG CATCTAAACA ACATGCCTTA 
CAATCATCCA GTGTTGACTC TGGCAATTGG AACACAAACA GCAGCCCTGG TGCCTTTCAG 
CCTTCCGTGG AACCTCTCCG GGCCGATAGC CACACCTTGC TGGAGGATCT GGGCTCTTTT 
CATGAGCTGA TGGCCACAGA AACGACGGCT AATGAATCTG AGCTGTCTGA GCACCCACTC 
ACAGCTGTCG GCAAAACCAA ACATGGCGAC TCTCAGCAGC TTCATGAATT GCCTGTATTG 
TACGAAGCAC TCGAACCGGC AGAAATAGAG GCATCTGTAG GGGACTATCC AGGCCTGGGC 
TCCGTGCCAC AGCATTCCAA CGGTGATGAA GGGGCAGAAG TGGAGTCTTC ATCTCTGACC 
TTGAAAAGCG ACACACTTAC TGAGGCAGAA TCCAGCGTAC ACCCAGATAG TTCATCTGCC 
ATCAGCTCTT CTGCATTCTC TTTGGGCAGC TTTTCTCTTC TCAGGGACAC ACACAATGCC 
CGAGGCATCA TGGAAGAACC TGAGTTATCG CTAGTGAGTT TCCCTGACAG CAGCTTTGCT 
GGTTCAGAGC ACGTTACCAC CTTGACTAGC ATGTCGGATC ACCACGTGGA CCCAAGCTTT 
GCAGAGGGTT GCTTTCATCC CCTACCAGCT GAAGTGGACC ACTCCATGTT TAACATGCCA 
GACTGGTCAG CAGCGGGACC AAGCTTTACT GACCAATCTA CACCACAGCA CTTTGCAGCA 
CTTGATTGGA CATCCCCTGT GAGCCTGCAG GAAGCCTTAT TAAAGAAGAA AAAACATTTT 
ATCGAAAGCT CTTCAAAACG AGTTGAACAA ATCAAGCAGA AAGCTGCGGC AGTCGGAAAC 
CCTGTATTTA ACTCCACGCC GAATGTTGGA GTCAGCGCGC AGGAGGAGGC GTCTGCCTGT 
GACGAAGCTT CAGCAAGTGA CAGCAGCACC CTGAAGAAGG TTGTAGAAGT TCGTGTGTGT 
ACCCCGGAGG AGCGCAAGCT GTCGGAGATA GAGATGCACC AGAGGACTAT AAGGTTGTAC 
AACCAGCTCG ACGAAGTTAA AACCAAAAAA GAGGAGAAGA TGAGAAAGGA GTCCTATGCC 
AGAAACAGAG AGAAGGCTAA CGAGTTTAAG AAGAAAACTC TGGAAAAGCT GAGATCCAGG 
AGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186852.1
CDS	74..5422
Translation	MKRKVANTGVIRLSPNEEALLLKQERERRRKLRLQQVREQEKFIALQIRHNVKERREQELQNLAEELRLEWQASQAEKLRALEKLYVSSLSAIGEGHRQAKENEPDLQAVERQAAANRQKAEKRHREALRELKQHKEKQLREQTWLLKARRKALITEKERAAKIASLPPPPPDPLENIEVLRRFPTVKLCNVDGFSVSHYHLPEPYVDRETDTEQPDARVAAEEEAKRLDALQKEEERERRERMEKAHLRGSHALKKVLLTQDRDKLLKELEQMQQEDLAQRRQVVAQMPQQLFEPAYRREEIQEEWQRELEVAFEDMYTGERRVRGDMVLHLKPQPLPVPSAESFDDDLDLSVDPEPVCETQPQTDAVEEPSHIEKTDDKSREPQSRIVFKRLLNKIRSQQDQWSAKSDVETQSDTVESGSLPNEIHTIHAELHEARKPAPEGIQNMSDSTILAGNAVPRHATEPGTPTKQASDWNKQDLERQKQEQLDLLNRLEEEKKRLEAEYLRVQLQMQKSKQEGMEPPEEEEEEEAAVIPPAVTESKTMEEPKPAQEPPGLSLNVSTESPHIQIIRQYQQRLIEQNRQHKQSVEEARQRLQEYQQLLKKRYSHLSASRLQPAAKEPVSFAIKPGLAQKPAQHLEDIGGASDSHVRVTSYPGESLAGHLVSLGSSLISAVPVSGQNLFAPSPVSRTAEFGQSGSSPLPHSTEQDNFIPYSSGTIPTQQQYGGAESMTGKDIAANGIKSPKRATPLGNFILEVAAPRLEQYTSSPEEDSISPPYSMYDLEKQSSTSSKGDYDTASDSFYALPSSLSLGLPQNIENIEQEPHVQKVDSVPFGEFSNVHEFRKGLISSTAKMQAQQTQLRDMQLQLEMQRESLLSKQKSQEEGLLQKQKELEDQMRKHQESLEHFLANKESAQGALPPDLCQISNNERLRLMSALLKALGDGKQENLSLGNGSSLRAAGRELKGRPSKPPVTKTKLGPSLEQHELSAILEVETPSGRPSSTGPPEHKNSSSGRQDGAEGGNSSVPPACMQENSRLREAGNYSLGDPDLSRTSTSSNESNRSQTKLSWRQMLSLAAASSHNLTLPDQSWSSSVHSGLSPHPAGTKTAFHFQQPAQERSDWLGAAELGKTGIGYSAQTQARDAFSDHLSTTTISTGSFLTSEKADSSISNLETISDLHKCNFSIINGAEHGNWSTSVNGVPQPTRRDEFPSPEYLSTAPDQRSHIQQIIDKYTRDLSASLERNLSFHTPTAALDVSGNDNHLPSGFYALEPKPDFNVSTTSHSQLNNTGSSQEAKNTSQSSFYPSSKEWSSEQSHSFQYPDSSHSPMSSPGITSAQHVDVEITPHVRGDQTLGSSGSFHPLHPECTLNEPPLSSPGLCGCSSAVDDLGNGSMKASKQHALQSSSVDSGNWNTNSSPGAFQPSVEPLRADSHTLLEDLGSFHELMATETTANESELSEHPLTAVGKTKHGDSQQLHELPVLYEALEPAEIEASVGDYPGLGSVPQHSNGDEGAEVESSSLTLKSDTLTEAESSVHPDSSSAISSSAFSLGSFSLLRDTHNARGIMEEPELSLVSFPDSSFAGSEHVTTLTSMSDHHVDPSFAEGCFHPLPAEVDHSMFNMPDWSAAGPSFTDQSTPQHFAALDWTSPVSLQEALLKKKKHFIESSSKRVEQIKQKAAAVGNPVFNSTPNVGVSAQEEASACDEASASDSSTLKKVVEVRVCTPEERKLSEIEMHQRTIRLYNQLDEVKTKKEEKMRKESYARNREKANEFKKKTLEKLRSRRK

Target ORF information:

RefSeq Version	NM_001199923.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis centrosomal protein 295 (cep295), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199923.1

ORF Insert Sequence:

ATGAAACGCA AAGTTGCAAA TACCGGCGTC ATAAGACTTA GCCCCAATGA AGAAGCCTTG 
CTGCTAAAGC AGGAACGTGA AAGAAGGCGC AAACTCCGCT TACAACAGGT TAGAGAACAG 
GAGAAGTTCA TTGCCCTGCA AATCCGTCAC AATGTTAAAG AAAGGAGGGA ACAGGAGCTT 
CAGAATTTGG CAGAGGAGCT GCGCTTGGAG TGGCAGGCAT CGCAGGCTGA GAAGCTCCGA 
GCCCTGGAGA AGCTTTATGT GTCCAGTCTA AGTGCGATTG GAGAGGGGCA CAGGCAAGCC 
AAGGAAAATG AACCGGACCT TCAAGCCGTT GAGAGGCAAG CGGCAGCCAA TAGACAGAAA 
GCAGAGAAGC GGCACAGAGA AGCTCTCAGG GAATTAAAGC AGCACAAAGA AAAACAATTG 
AGAGAGCAAA CCTGGCTTTT GAAAGCCCGC AGGAAAGCGC TTATTACTGA GAAGGAGCGT 
GCTGCAAAGA TAGCAAGCCT TCCTCCCCCA CCCCCAGACC CTCTAGAGAA TATTGAGGTT 
TTGCGAAGGT TCCCAACCGT GAAGCTGTGC AATGTTGATG GCTTCTCTGT ATCTCATTAT 
CATCTGCCTG AACCATACGT AGACCGAGAG ACGGATACGG AACAGCCAGA TGCTCGGGTT 
GCCGCTGAAG AGGAAGCAAA GCGTTTGGAT GCCTTACAGA AAGAGGAAGA GAGAGAAAGG 
AGGGAAAGGA TGGAGAAGGC TCACTTACGG GGATCCCATG CCTTGAAAAA GGTTCTTTTA 
ACCCAAGACC GGGACAAACT GCTGAAAGAA CTGGAACAGA TGCAGCAAGA GGATTTGGCT 
CAGAGGAGGC AGGTTGTCGC CCAGATGCCT CAGCAGCTAT TTGAACCGGC CTATAGGCGA 
GAAGAGATAC AGGAGGAGTG GCAGAGGGAA CTGGAGGTTG CTTTTGAAGA CATGTACACT 
GGAGAAAGAA GGGTTAGAGG GGACATGGTT TTGCACCTCA AGCCACAGCC CTTACCAGTT 
CCATCTGCTG AAAGTTTTGA TGATGACCTG GACCTGTCTG TGGATCCAGA GCCCGTGTGT 
GAAACCCAGC CACAAACTGA TGCTGTTGAG GAACCAAGTC ACATTGAGAA AACGGATGAC 
AAAAGCCGCG AGCCACAATC GAGAATTGTT TTTAAGAGAC TATTGAATAA AATACGAAGC 
CAGCAGGATC AGTGGAGCGC CAAAAGTGAT GTGGAGACGC AGAGCGACAC TGTGGAATCG 
GGATCTCTGC CTAATGAAAT ACATACAATC CATGCAGAAC TCCATGAGGC AAGGAAGCCT 
GCGCCTGAGG GCATTCAGAA CATGAGTGAT AGTACCATTC TAGCTGGTAA TGCAGTTCCT 
CGTCATGCAA CGGAGCCAGG AACACCAACA AAACAGGCCT CTGATTGGAA CAAGCAGGAT 
TTAGAGAGGC AGAAACAGGA GCAGCTGGAT TTGCTGAACA GACTAGAAGA GGAGAAAAAA 
CGCTTAGAAG CCGAATACTT GAGGGTTCAA CTCCAGATGC AAAAATCAAA GCAAGAAGGA 
ATGGAACCTC CAGAGGAGGA GGAGGAGGAA GAGGCAGCTG TAATTCCTCC TGCAGTGACT 
GAATCCAAAA CCATGGAAGA GCCCAAACCT GCGCAGGAAC CTCCAGGTTT AAGCCTAAAT 
GTTTCCACCG AGAGCCCGCA CATTCAGATA ATTCGACAGT ACCAGCAACG TCTAATTGAG 
CAAAACAGAC AACACAAGCA GTCGGTAGAA GAGGCGAGAC AACGTTTGCA AGAATACCAG 
CAATTGCTGA AAAAACGCTA TTCGCATTTG TCTGCCTCGA GGCTGCAGCC TGCAGCCAAG 
GAACCCGTTA GTTTCGCTAT AAAGCCCGGT CTCGCACAGA AACCTGCACA GCACTTGGAA 
GACATTGGCG GTGCTTCAGA CTCCCATGTT AGAGTAACCT CTTACCCTGG GGAGAGTCTA 
GCAGGGCACC TTGTATCTCT GGGTAGCTCT TTAATATCTG CAGTGCCGGT TTCTGGACAA 
AACTTATTTG CACCTTCACC AGTAAGCAGA ACAGCAGAGT TTGGACAGTC GGGAAGCAGT 
CCCCTCCCTC ATTCTACAGA GCAAGATAAC TTTATTCCAT ACTCTTCTGG GACTATTCCA 
ACACAACAAC AATATGGAGG GGCAGAAAGC ATGACCGGAA AAGACATTGC TGCGAACGGC 
ATTAAAAGTC CTAAAAGAGC AACACCGTTG GGAAATTTTA TTTTGGAAGT GGCAGCTCCC 
AGGCTAGAAC AATACACTTC TTCGCCCGAA GAAGACTCCA TCAGTCCTCC ATATTCTATG 
TACGATCTGG AAAAGCAGTC CTCCACATCA AGCAAAGGTG ATTATGATAC GGCCTCTGAT 
AGTTTCTACG CCCTTCCTTC TTCCTTGTCT TTGGGTCTGC CACAGAATAT AGAGAATATA 
GAGCAAGAAC CTCATGTTCA AAAAGTGGAT AGTGTTCCAT TCGGAGAGTT CTCTAATGTG 
CACGAGTTCC GCAAGGGGCT GATCTCTTCA ACGGCCAAGA TGCAAGCTCA GCAGACTCAG 
CTTAGGGATA TGCAGCTGCA GCTGGAGATG CAGAGGGAGT CCCTCTTGTC TAAACAGAAA 
TCCCAAGAAG AAGGTCTGTT ACAGAAACAG AAAGAGCTGG AAGATCAAAT GCGCAAGCAC 
CAAGAGTCGT TGGAACATTT CCTGGCCAAC AAGGAGTCTG CACAAGGGGC GTTACCTCCT 
GATCTCTGTC AGATTTCAAA TAACGAACGT CTTCGCCTTA TGTCTGCGCT GCTGAAAGCA 
CTGGGTGACG GTAAACAAGA GAACCTCAGT CTGGGAAATG GTAGTAGCCT TAGAGCTGCT 
GGGAGAGAAC TGAAAGGGAG ACCGTCTAAA CCTCCGGTTA CAAAAACCAA ATTGGGTCCA 
TCGTTGGAAC AACATGAGCT CAGTGCTATT CTGGAAGTGG AAACGCCAAG TGGCAGGCCT 
TCTAGTACTG GGCCGCCGGA ACACAAGAAC AGCAGCTCAG GCCGACAGGA TGGTGCCGAG 
GGTGGGAACT CGTCTGTGCC CCCTGCTTGC ATGCAAGAAA ACAGCAGACT GAGGGAAGCT 
GGTAACTATT CTCTCGGAGA TCCGGATCTT TCCAGAACAT CGACGAGCAG CAATGAAAGC 
AATAGGAGCC AGACTAAGTT ATCTTGGAGA CAGATGTTGT CTCTAGCAGC TGCTTCATCC 
CATAACTTGA CTCTCCCAGA TCAATCCTGG AGCTCTTCAG TGCACTCAGG CCTTTCTCCT 
CATCCTGCTG GTACCAAAAC AGCATTCCAC TTTCAGCAAC CTGCCCAGGA GAGATCCGAT 
TGGCTGGGGG CAGCTGAGCT GGGAAAAACA GGCATTGGAT ACAGTGCTCA AACGCAGGCC 
CGGGACGCTT TCAGCGATCA CTTGTCAACA ACCACAATCT CTACGGGAAG TTTTCTGACC 
AGCGAGAAGG CTGATTCAAG CATTAGCAAC TTAGAGACTA TTTCAGACCT TCACAAATGC 
AACTTCTCCA TTATCAATGG CGCGGAACAC GGCAACTGGA GTACCTCAGT CAACGGGGTT 
CCACAGCCGA CTAGAAGGGA TGAGTTCCCG AGCCCGGAAT ATTTGTCGAC TGCACCGGAT 
CAGCGCAGCC ACATTCAGCA GATCATTGAT AAATACACCA GGGATCTCAG TGCTTCCCTA 
GAGAGAAATC TAAGCTTTCA TACTCCTACT GCTGCTCTAG ATGTATCTGG GAATGACAAT 
CACCTGCCCT CGGGTTTCTA TGCCCTGGAA CCGAAACCTG ACTTCAATGT CTCTACAACT 
TCCCATTCAC AATTAAACAA CACCGGCAGC TCTCAAGAGG CAAAGAACAC AAGCCAGAGC 
TCGTTTTACC CAAGCAGCAA AGAGTGGTCT TCAGAACAAT CTCATAGCTT CCAGTATCCA 
GACAGCAGCC ATAGTCCGAT GTCTTCCCCT GGCATAACTA GTGCGCAGCA CGTGGATGTG 
GAAATCACCC CTCATGTTCG TGGGGATCAA ACTCTAGGTT CCTCTGGATC GTTCCACCCT 
CTGCACCCAG AATGCACCCT TAATGAGCCA CCGCTGAGCA GCCCAGGTCT CTGCGGATGC 
AGCAGTGCAG TTGATGATCT GGGAAATGGA AGCATGAAGG CATCTAAACA ACATGCCTTA 
CAATCATCCA GTGTTGACTC TGGCAATTGG AACACAAACA GCAGCCCTGG TGCCTTTCAG 
CCTTCCGTGG AACCTCTCCG GGCCGATAGC CACACCTTGC TGGAGGATCT GGGCTCTTTT 
CATGAGCTGA TGGCCACAGA AACGACGGCT AATGAATCTG AGCTGTCTGA GCACCCACTC 
ACAGCTGTCG GCAAAACCAA ACATGGCGAC TCTCAGCAGC TTCATGAATT GCCTGTATTG 
TACGAAGCAC TCGAACCGGC AGAAATAGAG GCATCTGTAG GGGACTATCC AGGCCTGGGC 
TCCGTGCCAC AGCATTCCAA CGGTGATGAA GGGGCAGAAG TGGAGTCTTC ATCTCTGACC 
TTGAAAAGCG ACACACTTAC TGAGGCAGAA TCCAGCGTAC ACCCAGATAG TTCATCTGCC 
ATCAGCTCTT CTGCATTCTC TTTGGGCAGC TTTTCTCTTC TCAGGGACAC ACACAATGCC 
CGAGGCATCA TGGAAGAACC TGAGTTATCG CTAGTGAGTT TCCCTGACAG CAGCTTTGCT 
GGTTCAGAGC ACGTTACCAC CTTGACTAGC ATGTCGGATC ACCACGTGGA CCCAAGCTTT 
GCAGAGGGTT GCTTTCATCC CCTACCAGCT GAAGTGGACC ACTCCATGTT TAACATGCCA 
GACTGGTCAG CAGCGGGACC AAGCTTTACT GACCAATCTA CACCACAGCA CTTTGCAGCA 
CTTGATTGGA CATCCCCTGT GAGCCTGCAG GAAGCCTTAT TAAAGAAGAA AAAACATTTT 
ATCGAAAGCT CTTCAAAACG AGTTGAACAA ATCAAGCAGA AAGCTGCGGC AGTCGGAAAC 
CCTGTATTTA ACTCCACGCC GAATGTTGGA GTCAGCGCGC AGGAGGAGGC GTCTGCCTGT 
GACGAAGCTT CAGCAAGTGA CAGCAGCACC CTGAAGAAGG TTGTAGAAGT TCGTGTGTGT 
ACCCCGGAGG AGCGCAAGCT GTCGGAGATA GAGATGCACC AGAGGACTAT AAGGTTGTAC 
AACCAGCTCG ACGAAGTTAA AACCAAAAAA GAGGAGAAGA TGAGAAAGGA GTCCTATGCC 
AGAAACAGAG AGAAGGCTAA CGAGTTTAAG AAGAAAACTC TGGAAAAGCT GAGATCCAGG 
AGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cep295 ( XM_012956604.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012812058.1 Xenopus tropicalis centrosomal protein 295kDa (cep295), transcript variant X1, mRNA.

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CloneID	OXa32965
Clone ID Related Accession (Same CDS sequence)	XM_012956604.2
Accession Version	XM_012956604.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5349bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	centrosomal protein of 295 kDa isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) and transcript sequence (NM_001199923.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012956604.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 45 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGAAACGCA AAGTTGCAAA TACCGGCGTC ATAAGACTTA GCCCCAATGA AGAAGCCTTG 
CTGCTAAAGC AGGAACGTGA AAGAAGGCGC AAACTCCGCT TACAACAGGT TAGAGAACAG 
GAGAAGTTCA TTGCCCTGCA AATCCGTCAC AATGTTAAAG AAAGGAGGGA ACAGGAGCTT 
CAGAATTTGG CAGAGGAGCT GCGCTTGGAG TGGCAGGCAT CGCAGGCTGA GAAGCTCCGA 
GCCCTGGAGA AGCTTTATGT GTCCAGTCTA AGTGCGATTG GAGAGGGGCA CAGGCAAGCC 
AAGGAAAATG AACCGGACCT TCAAGCCGTT GAGAGGCAAG CGGCAGCCAA TAGACAGAAA 
GCAGAGAAGC GGCACAGAGA AGCTCTCAGG GAATTAAAGC AGCACAAAGA AAAACAATTG 
AGAGAGCAAA CCTGGCTTTT GAAAGCCCGC AGGAAAGCGC TTATTACTGA GAAGGAGCGT 
GCTGCAAAGA TAGCAAGCCT TCCTCCCCCA CCCCCAGACC CTCTAGAGAA TATTGAGGTT 
TTGCGAAGGT TCCCAACCGT GAAGCTGTGC AATGTTGATG GCTTCTCTGT ATCTCATTAT 
CATCTGCCTG AACCATACGT AGACCGAGAG ACGGATACGG AACAGCCAGA TGCTCGGGTT 
GCCGCTGAAG AGGAAGCAAA GCGTTTGGAT GCCTTACAGA AAGAGGAAGA GAGAGAAAGG 
AGGGAAAGGA TGGAGAAGGC TCACTTACGG GGATCCCATG CCTTGAAAAA GGTTCTTTTA 
ACCCAAGACC GGGACAAACT GCTGAAAGAA CTGGAACAGA TGCAGCAAGA GGATTTGGCT 
CAGAGGAGGC AGGTTGTCGC CCAGATGCCT CAGCAGCTAT TTGAACCGGC CTATAGGCGA 
GAAGAGATAC AGGAGGAGTG GCAGAGGGAA CTGGAGGTTG CTTTTGAAGA CATGTACACT 
GGAGAAAGAA GGGTTAGAGG GGACATGGTT TTGCACCTCA AGCCACAGCC CTTACCAGTT 
CCATCTGCTG AAAGTTTTGA TGATGACCTG GACCTGTCTG TGGATCCAGA GCCCGTGTGT 
GAAACCCAGC CACAAACTGA TGCTGTTGAG GAACCAAGTC ACATTGAGAA AACGGATGAC 
AAAAGCCGCG AGCCACAATC GAGAATTGTT TTTAAGAGAC TATTGAATAA AATACGAAGC 
CAGCAGGATC AGTGGAGCGC CAAAAGTGAT GTGGAGACGC AGAGCGACAC TGTGGAATCG 
GGATCTCTGC CTAATGAAAT ACATACAATC CATGCAGAAC TCCATGAGGC AAGGAAGCCT 
GCGCCTGAGG GCATTCAGAA CATGAGTGAT AGTACCATTC TAGCTGGTAA TGCAGTTCCT 
CGTCATGCAA CGGAGCCAGG AACACCAACA AAACAGGCCT CTGATTGGAA CAAGCAGGAT 
TTAGAGAGGC AGAAACAGGA GCAGCTGGAT TTGCTGAACA GACTAGAAGA GGAGAAAAAA 
CGCTTAGAAG CCGAATACTT GAGGGTTCAA CTCCAGATGC AAAAATCAAA GCAAGAAGGA 
ATGGAACCTC CAGAGGAGGA GGAGGAGGAA GAGGCAGCTG TAATTCCTCC TGCAGTGACT 
GAATCCAAAA CCATGGAAGA GCCCAAACCT GCGCAGGAAC CTCCAGGTTT AAGCCTAAAT 
GTTTCCACCG AGAGCCCGCA CATTCAGATA ATTCGACAGT ACCAGCAACG TCTAATTGAG 
CAAAACAGAC AACACAAGCA GTCGGTAGAA GAGGCGAGAC AACGTTTGCA AGAATACCAG 
CAATTGCTGA AAAAACGCTA TTCGCATTTG TCTGCCTCGA GGCTGCAGCC TGCAGCCAAG 
GAACCCGTTA GTTTCGCTAT AAAGCCCGGT CTCGCACAGA AACCTGCACA GCACTTGGAA 
GACATTGGCG GTGCTTCAGA CTCCCATGTT AGAGTAACCT CTTACCCTGG GGAGAGTCTA 
GCAGGGCACC TTGTATCTCT GGGTAGCTCT TTAATATCTG CAGTGCCGGT TTCTGGACAA 
AACTTATTTG CACCTTCACC AGTAAGCAGA ACAGCAGAGT TTGGACAGTC GGGAAGCAGT 
CCCCTCCCTC ATTCTACAGA GCAAGATAAC TTTATTCCAT ACTCTTCTGG GACTATTCCA 
ACACAACAAC AATATGGAGG GGCAGAAAGC ATGACCGGAA AAGACATTGC TGCGAACGGC 
ATTAAAAGTC CTAAAAGAGC AACACCGTTG GGAAATTTTA TTTTGGAAGT GGCAGCTCCC 
AGGCTAGAAC AATACACTTC TTCGCCCGAA GAAGACTCCA TCAGTCCTCC ATATTCTATG 
TACGATCTGG AAAAGCAGTC CTCCACATCA AGCAAAGGTG ATTATGATAC GGCCTCTGAT 
AGTTTCTACG CCCTTCCTTC TTCCTTGTCT TTGGGTCTGC CACAGAATAT AGAGAATATA 
GAGCAAGAAC CTCATGTTCA AAAAGTGGAT AGTGTTCCAT TCGGAGAGTT CTCTAATGTG 
CACGAGTTCC GCAAGGGGCT GATCTCTTCA ACGGCCAAGA TGCAAGCTCA GCAGACTCAG 
CTTAGGGATA TGCAGCTGCA GCTGGAGATG CAGAGGGAGT CCCTCTTGTC TAAACAGAAA 
TCCCAAGAAG AAGGTCTGTT ACAGAAACAG AAAGAGCTGG AAGATCAAAT GCGCAAGCAC 
CAAGAGTCGT TGGAACATTT CCTGGCCAAC AAGGAGTCTG CACAAGGGGC GTTACCTCCT 
GATCTCTGTC AGATTTCAAA TAACGAACGT CTTCGCCTTA TGTCTGCGCT GCTGAAAGCA 
CTGGGTGACG GTAAACAAGA GAACCTCAGT CTGGGAAATG GTAGTAGCCT TAGAGCTGCT 
GGGAGAGAAC TGAAAGGGAG ACCGTCTAAA CCTCCGGTTA CAAAAACCAA ATTGGGTCCA 
TCGTTGGAAC AACATGAGCT CAGTGCTATT CTGGAAGTGG AAACGCCAAG TGGCAGGCCT 
TCTAGTACTG GGCCGCCGGA ACACAAGAAC AGCAGCTCAG GCCGACAGGA TGGTGCTGAG 
GGTGGGAACT CGTCTGTGCC CCCTGCTTGC ATGCAAGAAA ACAGCAGACT GAGGGAAGCC 
GGTAACTATT CTCTCGGAGA TCCGGATCTT TCCAGAACAT CGACGAGCAG CAATGAAAGC 
AATAGGAGCC AGACTAAGTT ATCTTGGAGA CAGATGTTGT CTCTAGCAGC TGCTTCATCC 
CATAACTTGA CTCTCCCAGA TCAATCCTGG AGCTCTTCAG TGCACTCAGG CCTTTCTCCT 
CATCCTGCTG GTACCAAAAC AGCATTCCAC TTTCAGCAAC CTGCCCAGGA GAGATCCGAT 
TGGCTGGGGG CAGCTGAGCT GGGAAAAACA GGCATTGGAT ACAGTGCTCA AACGCAGGCC 
CGGGACGCTT TCAGCGATCA CTTGTCAACA ACCACAATCT CTACGGGAAG TTTTCTGACC 
AGCGAGAAGG CTGATTCAAG CATTAGCAAC TTAGAGACTA TTTCAGACCT TCACAAATGC 
AACTTCTCCA TTATCAATGG CGCGGAACAC GGCAACTGGA GTACCTCAGT CAACGGGGTT 
CCACAGCCGA CTAGAAGGGA TGAGTTCCCG AGCCCGGAAT ATTTGTCGAC TGCACCGGAT 
CAGCGCAGCC ACATTCAGCA GATCATTGAT AAATACACCA GGGATCTCAG TGCTTCCCTA 
GAGAGAAATC TAAGCTTTCA TACTCCTACT GCTGCTCTAG ATGTATCTGG GAATGACAAT 
CACCTGCCCT CGGGTTTCTA TGCCCTGGAA CCGAAACCTG ACTTCAATGT CTCTACAACT 
TCCCATTCAC AATTAAACAA CACCGGCAGC TCTCAAGAGG CAAAGAACAC AAGCCAGAGC 
TCGTTTTACC CAAGCAGCAA AGAGTGGTCT TCAGAACAAT CTCATAGCTT CCAGTATCCA 
GACAGCAGCC ATAGTCCGAT GTCTTCCCCT GGCATAACTA GTGCGCAGCA CGTGGATGTG 
GAAATCACCC CTCATGTTCG TGGGGATCAA ACTCTAGGTT CCTCTGGATC GTTCCACCCT 
CTGCACCCAG AATGCACCCT TAATGAGCCA CCGCTGAGCA GCCCAGGTCT CTGCGGATGC 
AGCAGTGCAG TTGATGATCT GGGAAATGGA AGCATGAAGG CATCTAAACA ACATGCCTTA 
CAATCATCCA GTGTTGACTC TGGCAATTGG AACACAAACA GCAGCCCTGG TGCCTTTCAG 
CCTTCCGTGG AACCTCTCCG GGCCGATAGC CACACCTTGC TGGAGGATCT GGGCTCTTTT 
CATGAGCTGA TGGCCACAGA AACGACGGCT AATGAATCTG AGCTGTCTGA GCACCCACTC 
ACAGCTGTCG GCAAAACCAA ACATGGCGAC TCTCAGCAGC TTCATGAATT GCCTGTATTG 
TACGAAGCAC TCGAACCGGC AGAAATAGAG GCATCTGTAG GGGACTATCC AGGCCTGGGC 
TCCGTGCCAC AGCATTCCAA CGGTGATGAA GGGGCAGAAG TGGAGTCTTC ATCTCTGACC 
TTGAAAAGCG ACACACTTAC TGAGGCAGAA TCCAGCGTAC ACCCAGATAG TTCATCTGCC 
ATCAGCTCTT CTGCATTCTC TTTGGGCAGC TTTTCTCTTC TCAGGGACAC ACACAATGCC 
CGAGGCATCA TGGAAGAACC TGAGTTATCG CTAGTGAGTT TCCCTGACAG CAGCTTTGCT 
GGTTCAGAGC ACGTTACCAC CTTGACTAGC ATGTCGGATC ACCACGTGGA CCCAAGCTTT 
GCAGAGGGTT GCTTTCATCC CCTACCAGCT GAAGTGGACC ACTCCATGTT TAACATGCCA 
GACTGGTCAG CAGCGGGACC AAGCTTTACT GACCAATCTA CACCACAGCA CTTTGCAGCA 
CTTGATTGGA CATCCCCTGT GAGCCTGCAG GAAGCCTTAT TAAAGAAGAA AAAACATTTT 
ATCGAAAGCT CTTCAAAACG AGTTGAACAA ATCAAGCAGA AAGCTGCGGC AGTCGGAAAC 
CCTGTATTTA ACTCCACGCC GAATGTTGGA GTCAGCGCGC AGGAGGAGGC GTCTGCCTGT 
GACGAAGCTT CAGCAAGTGA CAGCAGCACC CTGAAGAAGG TTGTAGAAGT TCGTGTGTGT 
ACCCCGGAGG AGCGCAAGCT GTCGGAGATA GAGATGCACC AGAGGACTAT AAGGTTGTAC 
AACCAGCTCG ACGAAGTTAA AACCAAAAAA GAGGAGAAGA TGAGAAAGGA GTCCTATGCC 
AGAAACAGAG AGAAGGCTAA CGAGTTTAAG AAGAAAACTC TGGAAAAGCT GAGATCCAGG 
AGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012812058.1
CDS	145..5493
Translation	MKRKVANTGVIRLSPNEEALLLKQERERRRKLRLQQVREQEKFIALQIRHNVKERREQELQNLAEELRLEWQASQAEKLRALEKLYVSSLSAIGEGHRQAKENEPDLQAVERQAAANRQKAEKRHREALRELKQHKEKQLREQTWLLKARRKALITEKERAAKIASLPPPPPDPLENIEVLRRFPTVKLCNVDGFSVSHYHLPEPYVDRETDTEQPDARVAAEEEAKRLDALQKEEERERRERMEKAHLRGSHALKKVLLTQDRDKLLKELEQMQQEDLAQRRQVVAQMPQQLFEPAYRREEIQEEWQRELEVAFEDMYTGERRVRGDMVLHLKPQPLPVPSAESFDDDLDLSVDPEPVCETQPQTDAVEEPSHIEKTDDKSREPQSRIVFKRLLNKIRSQQDQWSAKSDVETQSDTVESGSLPNEIHTIHAELHEARKPAPEGIQNMSDSTILAGNAVPRHATEPGTPTKQASDWNKQDLERQKQEQLDLLNRLEEEKKRLEAEYLRVQLQMQKSKQEGMEPPEEEEEEEAAVIPPAVTESKTMEEPKPAQEPPGLSLNVSTESPHIQIIRQYQQRLIEQNRQHKQSVEEARQRLQEYQQLLKKRYSHLSASRLQPAAKEPVSFAIKPGLAQKPAQHLEDIGGASDSHVRVTSYPGESLAGHLVSLGSSLISAVPVSGQNLFAPSPVSRTAEFGQSGSSPLPHSTEQDNFIPYSSGTIPTQQQYGGAESMTGKDIAANGIKSPKRATPLGNFILEVAAPRLEQYTSSPEEDSISPPYSMYDLEKQSSTSSKGDYDTASDSFYALPSSLSLGLPQNIENIEQEPHVQKVDSVPFGEFSNVHEFRKGLISSTAKMQAQQTQLRDMQLQLEMQRESLLSKQKSQEEGLLQKQKELEDQMRKHQESLEHFLANKESAQGALPPDLCQISNNERLRLMSALLKALGDGKQENLSLGNGSSLRAAGRELKGRPSKPPVTKTKLGPSLEQHELSAILEVETPSGRPSSTGPPEHKNSSSGRQDGAEGGNSSVPPACMQENSRLREAGNYSLGDPDLSRTSTSSNESNRSQTKLSWRQMLSLAAASSHNLTLPDQSWSSSVHSGLSPHPAGTKTAFHFQQPAQERSDWLGAAELGKTGIGYSAQTQARDAFSDHLSTTTISTGSFLTSEKADSSISNLETISDLHKCNFSIINGAEHGNWSTSVNGVPQPTRRDEFPSPEYLSTAPDQRSHIQQIIDKYTRDLSASLERNLSFHTPTAALDVSGNDNHLPSGFYALEPKPDFNVSTTSHSQLNNTGSSQEAKNTSQSSFYPSSKEWSSEQSHSFQYPDSSHSPMSSPGITSAQHVDVEITPHVRGDQTLGSSGSFHPLHPECTLNEPPLSSPGLCGCSSAVDDLGNGSMKASKQHALQSSSVDSGNWNTNSSPGAFQPSVEPLRADSHTLLEDLGSFHELMATETTANESELSEHPLTAVGKTKHGDSQQLHELPVLYEALEPAEIEASVGDYPGLGSVPQHSNGDEGAEVESSSLTLKSDTLTEAESSVHPDSSSAISSSAFSLGSFSLLRDTHNARGIMEEPELSLVSFPDSSFAGSEHVTTLTSMSDHHVDPSFAEGCFHPLPAEVDHSMFNMPDWSAAGPSFTDQSTPQHFAALDWTSPVSLQEALLKKKKHFIESSSKRVEQIKQKAAAVGNPVFNSTPNVGVSAQEEASACDEASASDSSTLKKVVEVRVCTPEERKLSEIEMHQRTIRLYNQLDEVKTKKEEKMRKESYARNREKANEFKKKTLEKLRSRRK

Target ORF information:

RefSeq Version	XM_012956604.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis centrosomal protein 295kDa (cep295), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956604.2

ORF Insert Sequence:

ATGAAACGCA AAGTTGCAAA TACCGGCGTC ATAAGACTTA GCCCCAATGA AGAAGCCTTG 
CTGCTAAAGC AGGAACGTGA AAGAAGGCGC AAACTCCGCT TACAACAGGT TAGAGAACAG 
GAGAAGTTCA TTGCCCTGCA AATCCGTCAC AATGTTAAAG AAAGGAGGGA ACAGGAGCTT 
CAGAATTTGG CAGAGGAGCT GCGCTTGGAG TGGCAGGCAT CGCAGGCTGA GAAGCTCCGA 
GCCCTGGAGA AGCTTTATGT GTCCAGTCTA AGTGCGATTG GAGAGGGGCA CAGGCAAGCC 
AAGGAAAATG AACCGGACCT TCAAGCCGTT GAGAGGCAAG CGGCAGCCAA TAGACAGAAA 
GCAGAGAAGC GGCACAGAGA AGCTCTCAGG GAATTAAAGC AGCACAAAGA AAAACAATTG 
AGAGAGCAAA CCTGGCTTTT GAAAGCCCGC AGGAAAGCGC TTATTACTGA GAAGGAGCGT 
GCTGCAAAGA TAGCAAGCCT TCCTCCCCCA CCCCCAGACC CTCTAGAGAA TATTGAGGTT 
TTGCGAAGGT TCCCAACCGT GAAGCTGTGC AATGTTGATG GCTTCTCTGT ATCTCATTAT 
CATCTGCCTG AACCATACGT AGACCGAGAG ACGGATACGG AACAGCCAGA TGCTCGGGTT 
GCCGCTGAAG AGGAAGCAAA GCGTTTGGAT GCCTTACAGA AAGAGGAAGA GAGAGAAAGG 
AGGGAAAGGA TGGAGAAGGC TCACTTACGG GGATCCCATG CCTTGAAAAA GGTTCTTTTA 
ACCCAAGACC GGGACAAACT GCTGAAAGAA CTGGAACAGA TGCAGCAAGA GGATTTGGCT 
CAGAGGAGGC AGGTTGTCGC CCAGATGCCT CAGCAGCTAT TTGAACCGGC CTATAGGCGA 
GAAGAGATAC AGGAGGAGTG GCAGAGGGAA CTGGAGGTTG CTTTTGAAGA CATGTACACT 
GGAGAAAGAA GGGTTAGAGG GGACATGGTT TTGCACCTCA AGCCACAGCC CTTACCAGTT 
CCATCTGCTG AAAGTTTTGA TGATGACCTG GACCTGTCTG TGGATCCAGA GCCCGTGTGT 
GAAACCCAGC CACAAACTGA TGCTGTTGAG GAACCAAGTC ACATTGAGAA AACGGATGAC 
AAAAGCCGCG AGCCACAATC GAGAATTGTT TTTAAGAGAC TATTGAATAA AATACGAAGC 
CAGCAGGATC AGTGGAGCGC CAAAAGTGAT GTGGAGACGC AGAGCGACAC TGTGGAATCG 
GGATCTCTGC CTAATGAAAT ACATACAATC CATGCAGAAC TCCATGAGGC AAGGAAGCCT 
GCGCCTGAGG GCATTCAGAA CATGAGTGAT AGTACCATTC TAGCTGGTAA TGCAGTTCCT 
CGTCATGCAA CGGAGCCAGG AACACCAACA AAACAGGCCT CTGATTGGAA CAAGCAGGAT 
TTAGAGAGGC AGAAACAGGA GCAGCTGGAT TTGCTGAACA GACTAGAAGA GGAGAAAAAA 
CGCTTAGAAG CCGAATACTT GAGGGTTCAA CTCCAGATGC AAAAATCAAA GCAAGAAGGA 
ATGGAACCTC CAGAGGAGGA GGAGGAGGAA GAGGCAGCTG TAATTCCTCC TGCAGTGACT 
GAATCCAAAA CCATGGAAGA GCCCAAACCT GCGCAGGAAC CTCCAGGTTT AAGCCTAAAT 
GTTTCCACCG AGAGCCCGCA CATTCAGATA ATTCGACAGT ACCAGCAACG TCTAATTGAG 
CAAAACAGAC AACACAAGCA GTCGGTAGAA GAGGCGAGAC AACGTTTGCA AGAATACCAG 
CAATTGCTGA AAAAACGCTA TTCGCATTTG TCTGCCTCGA GGCTGCAGCC TGCAGCCAAG 
GAACCCGTTA GTTTCGCTAT AAAGCCCGGT CTCGCACAGA AACCTGCACA GCACTTGGAA 
GACATTGGCG GTGCTTCAGA CTCCCATGTT AGAGTAACCT CTTACCCTGG GGAGAGTCTA 
GCAGGGCACC TTGTATCTCT GGGTAGCTCT TTAATATCTG CAGTGCCGGT TTCTGGACAA 
AACTTATTTG CACCTTCACC AGTAAGCAGA ACAGCAGAGT TTGGACAGTC GGGAAGCAGT 
CCCCTCCCTC ATTCTACAGA GCAAGATAAC TTTATTCCAT ACTCTTCTGG GACTATTCCA 
ACACAACAAC AATATGGAGG GGCAGAAAGC ATGACCGGAA AAGACATTGC TGCGAACGGC 
ATTAAAAGTC CTAAAAGAGC AACACCGTTG GGAAATTTTA TTTTGGAAGT GGCAGCTCCC 
AGGCTAGAAC AATACACTTC TTCGCCCGAA GAAGACTCCA TCAGTCCTCC ATATTCTATG 
TACGATCTGG AAAAGCAGTC CTCCACATCA AGCAAAGGTG ATTATGATAC GGCCTCTGAT 
AGTTTCTACG CCCTTCCTTC TTCCTTGTCT TTGGGTCTGC CACAGAATAT AGAGAATATA 
GAGCAAGAAC CTCATGTTCA AAAAGTGGAT AGTGTTCCAT TCGGAGAGTT CTCTAATGTG 
CACGAGTTCC GCAAGGGGCT GATCTCTTCA ACGGCCAAGA TGCAAGCTCA GCAGACTCAG 
CTTAGGGATA TGCAGCTGCA GCTGGAGATG CAGAGGGAGT CCCTCTTGTC TAAACAGAAA 
TCCCAAGAAG AAGGTCTGTT ACAGAAACAG AAAGAGCTGG AAGATCAAAT GCGCAAGCAC 
CAAGAGTCGT TGGAACATTT CCTGGCCAAC AAGGAGTCTG CACAAGGGGC GTTACCTCCT 
GATCTCTGTC AGATTTCAAA TAACGAACGT CTTCGCCTTA TGTCTGCGCT GCTGAAAGCA 
CTGGGTGACG GTAAACAAGA GAACCTCAGT CTGGGAAATG GTAGTAGCCT TAGAGCTGCT 
GGGAGAGAAC TGAAAGGGAG ACCGTCTAAA CCTCCGGTTA CAAAAACCAA ATTGGGTCCA 
TCGTTGGAAC AACATGAGCT CAGTGCTATT CTGGAAGTGG AAACGCCAAG TGGCAGGCCT 
TCTAGTACTG GGCCGCCGGA ACACAAGAAC AGCAGCTCAG GCCGACAGGA TGGTGCTGAG 
GGTGGGAACT CGTCTGTGCC CCCTGCTTGC ATGCAAGAAA ACAGCAGACT GAGGGAAGCC 
GGTAACTATT CTCTCGGAGA TCCGGATCTT TCCAGAACAT CGACGAGCAG CAATGAAAGC 
AATAGGAGCC AGACTAAGTT ATCTTGGAGA CAGATGTTGT CTCTAGCAGC TGCTTCATCC 
CATAACTTGA CTCTCCCAGA TCAATCCTGG AGCTCTTCAG TGCACTCAGG CCTTTCTCCT 
CATCCTGCTG GTACCAAAAC AGCATTCCAC TTTCAGCAAC CTGCCCAGGA GAGATCCGAT 
TGGCTGGGGG CAGCTGAGCT GGGAAAAACA GGCATTGGAT ACAGTGCTCA AACGCAGGCC 
CGGGACGCTT TCAGCGATCA CTTGTCAACA ACCACAATCT CTACGGGAAG TTTTCTGACC 
AGCGAGAAGG CTGATTCAAG CATTAGCAAC TTAGAGACTA TTTCAGACCT TCACAAATGC 
AACTTCTCCA TTATCAATGG CGCGGAACAC GGCAACTGGA GTACCTCAGT CAACGGGGTT 
CCACAGCCGA CTAGAAGGGA TGAGTTCCCG AGCCCGGAAT ATTTGTCGAC TGCACCGGAT 
CAGCGCAGCC ACATTCAGCA GATCATTGAT AAATACACCA GGGATCTCAG TGCTTCCCTA 
GAGAGAAATC TAAGCTTTCA TACTCCTACT GCTGCTCTAG ATGTATCTGG GAATGACAAT 
CACCTGCCCT CGGGTTTCTA TGCCCTGGAA CCGAAACCTG ACTTCAATGT CTCTACAACT 
TCCCATTCAC AATTAAACAA CACCGGCAGC TCTCAAGAGG CAAAGAACAC AAGCCAGAGC 
TCGTTTTACC CAAGCAGCAA AGAGTGGTCT TCAGAACAAT CTCATAGCTT CCAGTATCCA 
GACAGCAGCC ATAGTCCGAT GTCTTCCCCT GGCATAACTA GTGCGCAGCA CGTGGATGTG 
GAAATCACCC CTCATGTTCG TGGGGATCAA ACTCTAGGTT CCTCTGGATC GTTCCACCCT 
CTGCACCCAG AATGCACCCT TAATGAGCCA CCGCTGAGCA GCCCAGGTCT CTGCGGATGC 
AGCAGTGCAG TTGATGATCT GGGAAATGGA AGCATGAAGG CATCTAAACA ACATGCCTTA 
CAATCATCCA GTGTTGACTC TGGCAATTGG AACACAAACA GCAGCCCTGG TGCCTTTCAG 
CCTTCCGTGG AACCTCTCCG GGCCGATAGC CACACCTTGC TGGAGGATCT GGGCTCTTTT 
CATGAGCTGA TGGCCACAGA AACGACGGCT AATGAATCTG AGCTGTCTGA GCACCCACTC 
ACAGCTGTCG GCAAAACCAA ACATGGCGAC TCTCAGCAGC TTCATGAATT GCCTGTATTG 
TACGAAGCAC TCGAACCGGC AGAAATAGAG GCATCTGTAG GGGACTATCC AGGCCTGGGC 
TCCGTGCCAC AGCATTCCAA CGGTGATGAA GGGGCAGAAG TGGAGTCTTC ATCTCTGACC 
TTGAAAAGCG ACACACTTAC TGAGGCAGAA TCCAGCGTAC ACCCAGATAG TTCATCTGCC 
ATCAGCTCTT CTGCATTCTC TTTGGGCAGC TTTTCTCTTC TCAGGGACAC ACACAATGCC 
CGAGGCATCA TGGAAGAACC TGAGTTATCG CTAGTGAGTT TCCCTGACAG CAGCTTTGCT 
GGTTCAGAGC ACGTTACCAC CTTGACTAGC ATGTCGGATC ACCACGTGGA CCCAAGCTTT 
GCAGAGGGTT GCTTTCATCC CCTACCAGCT GAAGTGGACC ACTCCATGTT TAACATGCCA 
GACTGGTCAG CAGCGGGACC AAGCTTTACT GACCAATCTA CACCACAGCA CTTTGCAGCA 
CTTGATTGGA CATCCCCTGT GAGCCTGCAG GAAGCCTTAT TAAAGAAGAA AAAACATTTT 
ATCGAAAGCT CTTCAAAACG AGTTGAACAA ATCAAGCAGA AAGCTGCGGC AGTCGGAAAC 
CCTGTATTTA ACTCCACGCC GAATGTTGGA GTCAGCGCGC AGGAGGAGGC GTCTGCCTGT 
GACGAAGCTT CAGCAAGTGA CAGCAGCACC CTGAAGAAGG TTGTAGAAGT TCGTGTGTGT 
ACCCCGGAGG AGCGCAAGCT GTCGGAGATA GAGATGCACC AGAGGACTAT AAGGTTGTAC 
AACCAGCTCG ACGAAGTTAA AACCAAAAAA GAGGAGAAGA TGAGAAAGGA GTCCTATGCC 
AGAAACAGAG AGAAGGCTAA CGAGTTTAAG AAGAAAACTC TGGAAAAGCT GAGATCCAGG 
AGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Eメール

japanmarket@genscript.com

電話

03-6811-6572

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03-6811-6573
050-3174-7589(受注専用)

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オンライン注文

cep295 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報