uspl1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	uspl1
Entrez Gene ID	100379747
Full Name	ubiquitin specific peptidase like 1
General protein information	Preferred Names ubiquitin specific peptidase like 1 Names SUMO-specific isopeptidase USPL1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018091733.2	XP_017947222.2	SUMO-specific isopeptidase USPL1 isoform X1
XM_018091733.1	XP_017947222.1	SUMO-specific isopeptidase USPL1 isoform X1
XM_018091734.2	XP_017947223.2	SUMO-specific isopeptidase USPL1 isoform X2
XM_018091731.2	XP_017947220.2	SUMO-specific isopeptidase USPL1 isoform X1
XM_018091731.1	XP_017947220.1	SUMO-specific isopeptidase USPL1 isoform X1
XM_018091732.1	XP_017947221.1	SUMO-specific isopeptidase USPL1 isoform X1
XM_018091734.1	XP_017947223.1	SUMO-specific isopeptidase USPL1 isoform X2

Pathways from BioSystems
Gene Ontology

The following uspl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the uspl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa52820	XM_018091733.2 Latest version!	Xenopus tropicalis ubiquitin specific peptidase like 1 (uspl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa52820	XM_018091731.2 Latest version!	Xenopus tropicalis ubiquitin specific peptidase like 1 (uspl1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa52821	XM_018091734.2 Latest version!	Xenopus tropicalis ubiquitin specific peptidase like 1 (uspl1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa42866	XM_018091733.1 Latest version!	Xenopus tropicalis ubiquitin specific peptidase like 1 (uspl1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OXa42866	XM_018091732.1 Latest version!	Xenopus tropicalis ubiquitin specific peptidase like 1 (uspl1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OXa42866	XM_018091731.1 Latest version!	Xenopus tropicalis ubiquitin specific peptidase like 1 (uspl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OXa42867	XM_018091734.1 Latest version!	Xenopus tropicalis ubiquitin specific peptidase like 1 (uspl1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

uspl1 ( XM_018091733.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947222.2 Xenopus tropicalis ubiquitin specific peptidase like 1 (uspl1), transcript variant X1, mRNA.

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CloneID	OXa52820
Clone ID Related Accession (Same CDS sequence)	XM_018091733.2 , XM_018091731.2
Accession Version	XM_018091733.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	SUMO-specific isopeptidase USPL1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018091733.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTAAAG TAAGCCAATG GTTCACAACG ATGGCTTCAG AGACTCCTGG ATCTACAGTG 
TCACTGGTTG GACAAGGGTC TATTTTAGAT AAATCTTCAC TCCACACGGT GGGGTGCTTG 
GGAAAAAATG TTGACCCCTG TGCAGCTCCT GCTGATGGAT TTTGCCCAGT ATGCAAGGCC 
AAAGGCCAGC TGCAAGCTCT CCGGACATAT CGCTTTAATT TCACTCAGTC TATATATCTC 
TGTGCCAATC CACAGTGCAT ATATCCCTTG GGATATACAC CTCTGGATAA CATAATTGCA 
AACACAGCAG ATCTACAGAA ACACCATAGC CCAGGAAAGC AGAAGAAGCG GTGTCTTTTA 
GACACCTCTG TAACAACTAA CTGTGTGAAG AAGCATAAGA CTGATTCTTT TGTCTCTGGA 
GAGTACATTT CAGAAAACCT ATTTTTATCT GCACAAAATA AAGGCTACAA ATCAATGTCT 
CCGTGTTTAC CTAAAGAATC TCATACAAGT GTACCTGGCC CTTCAGAGAA TGGTCAGGAT 
GAGAGAAGTC AGCAGCAATA TGGAATGTGC AAAGTTTCAG CTTGCAATTC TGCAAGTCCA 
CATGGTGACA GGGCACCTTC AGTTGAATTT GCTCTGCGGG ACTCAAACCA ACCTAATCAC 
CCGCAGGAAG CCTTAAATGC ATTTCACGAA GAAGCTGTGC AAAATCAAGT TATGGGAATG 
ACCATTGCAT CAATTAATGG TCCTTCTCCT TTAAAATGGA ATGTACCTGC TACTAAAGAT 
ATGCACACAT TATCACAGTC GGTGAATGCT GCTAATGACC CACCAACATG TATAAACCAA 
AAGACAGATG CTGGAATAGC TGAGGTTTTA AATGGATTTA CAAAGGCTGA AGCCAATATG 
TGCCATTCAG TATCTGTCCT TAGTACTTGT GCACCTTTAG GCTCACCTCG TTTAAGGCTT 
AGTGCTCTAA AGGAGGGACA TAAACAGAGT GATATACGGA GTGCCTCCTG CAGTAGTGCT 
TGTCTTGAAA AAGTAGCATC AGAAACAGCT CTTGCCGCCA TGTCTGACAG CAACATGAAG 
AAAGAGGATA GTTTCAACAT GGCATTTGAA CTTTTACCGT CGTCTCCTCC ATTGCATTCA 
TTTTTTGGGG CTTCACAAAA TGTGCACACA TCTGAAGAAT CATCCTCTTT GCTTGGAACA 
GTGGGTCAAA CAAAAGAAAA TACCATAAGT GTTAAATCAA TTGGATCCAG CAATGTTGTT 
CTTAATGAAG GAAAGGGGGA AACCTCCCAG TTTACGATAT TGTCCAAATC ACACAGTGAG 
GTAGAAGATG TTCAGAAAGT GTGTGTACGT TTGCCCTCTT CCCCACTGCT TGAAACAGCT 
TTACAAATGA AAGCGTATGC CCACAGTGAC AAGCTCAGTG CCTTCAACAA TAATCCATGT 
CTTGAGAAAG TATTACCAGA GGCAACCCCA GTGGTTTTAT CAGAAACATA TAGGGAGGTA 
CAAGATTGTT TTGGATCTTT ACCTTCATCT GATTTGCATG AAAATGAGCC TCCACTGGAA 
GAGAGATCCC TGGTTGATAA ATCTAATAGC TCCAGAAATT ATACTTGCCT TGAGAAAGTA 
GTACCAGAGG CAACCTTATT GTTAAAAGAG TACTTTTCAG AATCCTGTAG TAAGGTACAA 
GATGATGAGA AATGTTTTGG ATCTTTACCT TCATCTGCTT TGCAAAGAAA AAATCCTCTA 
ATTGAAAAAA ACGGACCGAG TGACAAATCC AGTGCATCCA GCAATAATGT TTGCCTTGAG 
GAAGGAGAAC CAGAGGCAGT CCCATTGTTG GAAGACTGTA AAAGTCAAAC CCTTGTTGTA 
CCTTCCACAT GCAGTCAATT GTCCATAACA GATAAAATAG CAAACAGTCT TGGAGTACAT 
AATTCCTCAG ATTCATGTCA GCTCATCAGT GAAATTCATC ATGATCATCC AAGCGATGGA 
AATGATCTCA CTGTGTCCCA TATTGCCTTC AATGTTGGTG GTGCTGACAA AATCAATAAA 
GTTAAAAGCC TGCAGGTAAC TCCACTTCCA GAGGTTATAT CTACAGAACT ATGTAGGGGC 
AAGAGCAACA GTCAAAGCAC TTGTGAACCT CCATCCGTGC CAGTTCTGTC ATGTAAGCTT 
TCTTCTGAGC AAAAAGATGA AGGTACATTG ATGGAATTGT CTAGCTCTTT ACATAGTGAA 
ACAACTGAGG AGGAAGAATG TGGAAATAAA ATGGCTCTTG GAAGTTCAAC ATTGGATGGG 
GAAATATCTG ACTATAAGAA ACTAAACAGT CAAATTAAGC CTTTGCCACA AGCAATAGAA 
AGCTTGGTCA TGGACACGTG TGATAGCAAT CTGTGCAATA ATATTAATTT AAAAGATAGC 
AGCAAAATAA TTTCAGAGGA AGCTACAGTA CCACATTGTT TTCAGCCAGA ACAGCATGTT 
GTATACAGCC ATCCTATGTC TGACCCTGCA GTTACTTGTG TGACTCATGG CACTGGATCT 
GAAATGAAAG CATTGGATAC ACCAAGGAAG GAGTCTAATC CAGTGTGTAA GGAACTCCGT 
GAAAGAAATG TAAATCCAAT GAAAGAATTG TATATATCTC TGAACAATGC ACCAACTGAT 
TTGTCTAACT CTTTGTGCAG TGACACTACT AATGTGGAAG AATCTAGGGA TGCCACAGCA 
AATGCAAGTT CTTTATCCAA CGGAGAAATA TTGCATTTAT ATCACAAAAC AAGCAGCCCA 
GCTCTTGTTG ATCCAAAAGC AGAAGCAAAT ACAGTTTTAG GCTTGTGTGA CAGTGTCCTC 
AGCAAAAACT CTACAATGGG AAGTAGTGAT AGCATTAATC AGGAGAAAGC TATAGCAACA 
ACTTTTGCAC ACCCAGGACA GAGTGTGTTG TTCAGCCATG GGGCATCTAC AATTACAGTC 
TCCCCTGGTT TATTTCATAG TGCTGTACAG GCATTAGATG TTTCTGATAC ACCAGAGTTG 
GGGTGCAGTC CTGTGTGTAA GGAACCCACT GTTGTACAAG ATAAAAGCGA TGGATCCTTT 
GATACTTCTT TGGGCCATTT TGAAAGAGAA AAGCAAAATG AGAGCTTTGA AACAGACAGT 
TCTGTAATGC TTACTGATGA TCACATAAAG GACCCAAAGG ATGTAGATGT TGTATTGTCT 
TTGCCAGAGG GGAGCTTGGA AGATTGCTGT CCCAAAAAAA AATCAAACTG CTCAGAGAAC 
ATGCATGTAG CCTTAAAGGG TACCTCCACA AACCCTTCCC TTGAACTCGG TTCCCACGAT 
GCCCCGTGTG AAAAGAAGCT GCTTCAATGG AGAAACACCC TCTTCCTCTG CTGGTTGGAC 
TGCATATTGG CAGCACTGGT GCATTCTGCT AGCCTGAATA GCATTGTGGC ACAGCGAAGG 
GTTGCAGAAA GCTCAATCAT TCACCTTCTT CTGGAGAAAT ACAAGGAGGC CAATGCATTA 
ATAAAAAGCA CATCCAGACG AGGACGTCCA AAGAATTTAT CTCAAGCTGA AAACATCCTG 
AATAAAGTGC GAATGACCAT CTTTGATGAA ATAAAGCCCT TGTTAAAGTG TGAATTGGGA 
GATAAAGAGA GCCCTGTGTT TGCTTTGCCG TTACTGCTGC AGCTGGTTCC AGAGATAGAA 
TCCTGTTTTG TGCACTCAGG TGTATGGGAG TTTACATGCA AGCTATGTGG CTACCAATAC 
CAAAATAAGC ACCAACGGAC TGTGTCCACA TTCACTAAGA TCTTACCTGA ATGGACACCC 
CTAAATGCTA TCCATAAAGC TCCTTGCAAC AAATGCCTGG GTACAGATCA GATACGTGCT 
TTTCGTATTA AAAAGATCCA TGACCTATTC ATACTTCACT TTGTGGAAGG CCTGCCAAGC 
AATGATTTAG GCATGTACTC GTTTCACTTT GAAGGCTGCC TGTATGAAGT AAAGACTGTC 
ATCCAGTATA GTGAAGACCA TTTCTCATCT TGGATTGCTA ATGAGGATGG CACATGGCTG 
GAATCTGATG ACCTGAAAGG GTATTTCTGC ATAAAACAGA ACTGTTTTGA GGTTCCTGCT 
TCAGAAATTC ATATTGTAAT ATGGGAAAAG AAGATTACCA ACAGCACTGG AGCACACCAT 
GCAATGGAAC CTGAAACAAA AAACAAAGCA CTTAATTCTT TATCTACTTC ACCTTCATCT 
TTATCTCAGA CCCTGCACCT GTCTCCTTCA GCTGCTCCGA ATGCTGCATC TTCACCTCTA 
AGAGATGCTC CAAATGCTTC CAGCGTACTC TCTGGAATGG AGGGTTTTCA TGATGACGAC 
ATCATAACGC TCACTCTCAT TGAAATTCCA ATTGATGCAT CGGGTAATGT GATTGAGAAC 
AACTCTGAGG CAGCCCAGCA GCCAAGTCTC ATTGCTGAAA CACATTCATC CCCTGTAACC 
CTCAAAGCAG TTGCTGATGG GCAATGTGAT CAAAATCTAT GTTCTGGTAC AATGAAGGAA 
AGCTCTTTCG CTGGTGGTTC TCTTCCAGTT CCTAACATTT CCAGCCCTCC TGCCCCTAAC 
ATTTCCAGCC CTCCTGCCCC TAACATTTCC AGCCCTCCTG CCCCTCACAT TTCCAGCCCT 
CCTGCCCCTC ACATTTCCAG CCCTCCTGCC CCTCACATTT CCAGCCCTCC TGCCCCTCAC 
ATTTCCAGCC CTCCTGCCCC TCACATTTCC AGCCCTCCTG CCCCTCACAT TTCCAGCCCT 
CCTGCCCCTA ACAGCGCCAG TCCCCCTGCC CCTAACAGCG CCAGTACTCC CCTCCCTAAC 
AGTTCCAGTT CAAAAAAAGC CTTTGTTGGA AGTTGGATGA AGAGTCTACT AAATAAAAAC 
CCTTTGTTCC TGACATCTAA TGTGCCCTGT GCAAATAAGA AAACTGTGGC AAAAACTCCA 
ATCCGATTTC CCCTGCTGAA GACAACAGAT TTGCAGGCAT CTACTAAACA GGCCCAGAGC 
TTTGATGGTT TTAAAGCTAA ACGTGTCGGT AATTCATTCA GCTCGGACAA TTGCCTTGGT 
GTAGGTCCCA CGAAGGATAA TTCCACTGCA CCCAAACCTG CCATCTCTAG CCAAACGTTC 
CAGCCACTGG TTTCAAATCG ACTTAAAAAT GGGCGGTTAG GACTCGGTAA TACCAAAGAC 
TTGGCAAAAA ATGTTTCTCT GTCTAAGGAG GATAAGGTGC TTAGGCTTAG ACTGAAAATG 
CTGAAAAAGT TGAAGGCTAA GAAACATGAA TTGGCCGCCA TAGATATGCT TGGAAAGTCA 
CAGGCTGGTA GTGATCTGGA GCGTAGCAGC ACCAGCAGGC GTGAACAATT ACGCGGGTTC 
TTGCAGGAAC TCCAAGAGCA AATCGATAAT GCCGACACTG AATCAGTGTG CACTATGAGC 
TCTTGCACGT CGATATGCAG CAGCCCAGGA GATGCGGAAT TCTTTGCGGA ATTGTTCTCC 
CCACCCCCTA GCAACGCCCC GCAGGAGAGC ATGGATGACA GTAATTTTTT AGAGATGTAT 
GTAGATGGTT ACAACCCCTC CTGTGGCAGT TATGGAACCA GTAACCCAAG TGGCAAAGAG 
GTCTCACAGG ACAGTGCTAA TATCGGCCAG TCCAATTTAC AGCGTGCTTC CGACAGCCAC 
AATAACACTA ACGTGGGGGA CGTCTTCCAT GATATGCTGT CAACCTCCAC AATGCACATG 
CTGAACGAGG ATTATTTCTC ACCTTTTGAT GATATGTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947222.2
CDS	362..6103
Translation	MTKVSQWFTTMASETPGSTVSLVGQGSILDKSSLHTVGCLGKNVDPCAAPADGFCPVCKAKGQLQALRTYRFNFTQSIYLCANPQCIYPLGYTPLDNIIANTADLQKHHSPGKQKKRCLLDTSVTTNCVKKHKTDSFVSGEYISENLFLSAQNKGYKSMSPCLPKESHTSVPGPSENGQDERSQQQYGMCKVSACNSASPHGDRAPSVEFALRDSNQPNHPQEALNAFHEEAVQNQVMGMTIASINGPSPLKWNVPATKDMHTLSQSVNAANDPPTCINQKTDAGIAEVLNGFTKAEANMCHSVSVLSTCAPLGSPRLRLSALKEGHKQSDIRSASCSSACLEKVASETALAAMSDSNMKKEDSFNMAFELLPSSPPLHSFFGASQNVHTSEESSSLLGTVGQTKENTISVKSIGSSNVVLNEGKGETSQFTILSKSHSEVEDVQKVCVRLPSSPLLETALQMKAYAHSDKLSAFNNNPCLEKVLPEATPVVLSETYREVQDCFGSLPSSDLHENEPPLEERSLVDKSNSSRNYTCLEKVVPEATLLLKEYFSESCSKVQDDEKCFGSLPSSALQRKNPLIEKNGPSDKSSASSNNVCLEEGEPEAVPLLEDCKSQTLVVPSTCSQLSITDKIANSLGVHNSSDSCQLISEIHHDHPSDGNDLTVSHIAFNVGGADKINKVKSLQVTPLPEVISTELCRGKSNSQSTCEPPSVPVLSCKLSSEQKDEGTLMELSSSLHSETTEEEECGNKMALGSSTLDGEISDYKKLNSQIKPLPQAIESLVMDTCDSNLCNNINLKDSSKIISEEATVPHCFQPEQHVVYSHPMSDPAVTCVTHGTGSEMKALDTPRKESNPVCKELRERNVNPMKELYISLNNAPTDLSNSLCSDTTNVEESRDATANASSLSNGEILHLYHKTSSPALVDPKAEANTVLGLCDSVLSKNSTMGSSDSINQEKAIATTFAHPGQSVLFSHGASTITVSPGLFHSAVQALDVSDTPELGCSPVCKEPTVVQDKSDGSFDTSLGHFEREKQNESFETDSSVMLTDDHIKDPKDVDVVLSLPEGSLEDCCPKKKSNCSENMHVALKGTSTNPSLELGSHDAPCEKKLLQWRNTLFLCWLDCILAALVHSASLNSIVAQRRVAESSIIHLLLEKYKEANALIKSTSRRGRPKNLSQAENILNKVRMTIFDEIKPLLKCELGDKESPVFALPLLLQLVPEIESCFVHSGVWEFTCKLCGYQYQNKHQRTVSTFTKILPEWTPLNAIHKAPCNKCLGTDQIRAFRIKKIHDLFILHFVEGLPSNDLGMYSFHFEGCLYEVKTVIQYSEDHFSSWIANEDGTWLESDDLKGYFCIKQNCFEVPASEIHIVIWEKKITNSTGAHHAMEPETKNKALNSLSTSPSSLSQTLHLSPSAAPNAASSPLRDAPNASSVLSGMEGFHDDDIITLTLIEIPIDASGNVIENNSEAAQQPSLIAETHSSPVTLKAVADGQCDQNLCSGTMKESSFAGGSLPVPNISSPPAPNISSPPAPNISSPPAPHISSPPAPHISSPPAPHISSPPAPHISSPPAPHISSPPAPHISSPPAPNSASPPAPNSASTPLPNSSSSKKAFVGSWMKSLLNKNPLFLTSNVPCANKKTVAKTPIRFPLLKTTDLQASTKQAQSFDGFKAKRVGNSFSSDNCLGVGPTKDNSTAPKPAISSQTFQPLVSNRLKNGRLGLGNTKDLAKNVSLSKEDKVLRLRLKMLKKLKAKKHELAAIDMLGKSQAGSDLERSSTSRREQLRGFLQELQEQIDNADTESVCTMSSCTSICSSPGDAEFFAELFSPPPSNAPQESMDDSNFLEMYVDGYNPSCGSYGTSNPSGKEVSQDSANIGQSNLQRASDSHNNTNVGDVFHDMLSTSTMHMLNEDYFSPFDDMF

Target ORF information:

RefSeq Version	XM_018091733.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin specific peptidase like 1 (uspl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018091733.2

ORF Insert Sequence:

ATGACTAAAG TAAGCCAATG GTTCACAACG ATGGCTTCAG AGACTCCTGG ATCTACAGTG 
TCACTGGTTG GACAAGGGTC TATTTTAGAT AAATCTTCAC TCCACACGGT GGGGTGCTTG 
GGAAAAAATG TTGACCCCTG TGCAGCTCCT GCTGATGGAT TTTGCCCAGT ATGCAAGGCC 
AAAGGCCAGC TGCAAGCTCT CCGGACATAT CGCTTTAATT TCACTCAGTC TATATATCTC 
TGTGCCAATC CACAGTGCAT ATATCCCTTG GGATATACAC CTCTGGATAA CATAATTGCA 
AACACAGCAG ATCTACAGAA ACACCATAGC CCAGGAAAGC AGAAGAAGCG GTGTCTTTTA 
GACACCTCTG TAACAACTAA CTGTGTGAAG AAGCATAAGA CTGATTCTTT TGTCTCTGGA 
GAGTACATTT CAGAAAACCT ATTTTTATCT GCACAAAATA AAGGCTACAA ATCAATGTCT 
CCGTGTTTAC CTAAAGAATC TCATACAAGT GTACCTGGCC CTTCAGAGAA TGGTCAGGAT 
GAGAGAAGTC AGCAGCAATA TGGAATGTGC AAAGTTTCAG CTTGCAATTC TGCAAGTCCA 
CATGGTGACA GGGCACCTTC AGTTGAATTT GCTCTGCGGG ACTCAAACCA ACCTAATCAC 
CCGCAGGAAG CCTTAAATGC ATTTCACGAA GAAGCTGTGC AAAATCAAGT TATGGGAATG 
ACCATTGCAT CAATTAATGG TCCTTCTCCT TTAAAATGGA ATGTACCTGC TACTAAAGAT 
ATGCACACAT TATCACAGTC GGTGAATGCT GCTAATGACC CACCAACATG TATAAACCAA 
AAGACAGATG CTGGAATAGC TGAGGTTTTA AATGGATTTA CAAAGGCTGA AGCCAATATG 
TGCCATTCAG TATCTGTCCT TAGTACTTGT GCACCTTTAG GCTCACCTCG TTTAAGGCTT 
AGTGCTCTAA AGGAGGGACA TAAACAGAGT GATATACGGA GTGCCTCCTG CAGTAGTGCT 
TGTCTTGAAA AAGTAGCATC AGAAACAGCT CTTGCCGCCA TGTCTGACAG CAACATGAAG 
AAAGAGGATA GTTTCAACAT GGCATTTGAA CTTTTACCGT CGTCTCCTCC ATTGCATTCA 
TTTTTTGGGG CTTCACAAAA TGTGCACACA TCTGAAGAAT CATCCTCTTT GCTTGGAACA 
GTGGGTCAAA CAAAAGAAAA TACCATAAGT GTTAAATCAA TTGGATCCAG CAATGTTGTT 
CTTAATGAAG GAAAGGGGGA AACCTCCCAG TTTACGATAT TGTCCAAATC ACACAGTGAG 
GTAGAAGATG TTCAGAAAGT GTGTGTACGT TTGCCCTCTT CCCCACTGCT TGAAACAGCT 
TTACAAATGA AAGCGTATGC CCACAGTGAC AAGCTCAGTG CCTTCAACAA TAATCCATGT 
CTTGAGAAAG TATTACCAGA GGCAACCCCA GTGGTTTTAT CAGAAACATA TAGGGAGGTA 
CAAGATTGTT TTGGATCTTT ACCTTCATCT GATTTGCATG AAAATGAGCC TCCACTGGAA 
GAGAGATCCC TGGTTGATAA ATCTAATAGC TCCAGAAATT ATACTTGCCT TGAGAAAGTA 
GTACCAGAGG CAACCTTATT GTTAAAAGAG TACTTTTCAG AATCCTGTAG TAAGGTACAA 
GATGATGAGA AATGTTTTGG ATCTTTACCT TCATCTGCTT TGCAAAGAAA AAATCCTCTA 
ATTGAAAAAA ACGGACCGAG TGACAAATCC AGTGCATCCA GCAATAATGT TTGCCTTGAG 
GAAGGAGAAC CAGAGGCAGT CCCATTGTTG GAAGACTGTA AAAGTCAAAC CCTTGTTGTA 
CCTTCCACAT GCAGTCAATT GTCCATAACA GATAAAATAG CAAACAGTCT TGGAGTACAT 
AATTCCTCAG ATTCATGTCA GCTCATCAGT GAAATTCATC ATGATCATCC AAGCGATGGA 
AATGATCTCA CTGTGTCCCA TATTGCCTTC AATGTTGGTG GTGCTGACAA AATCAATAAA 
GTTAAAAGCC TGCAGGTAAC TCCACTTCCA GAGGTTATAT CTACAGAACT ATGTAGGGGC 
AAGAGCAACA GTCAAAGCAC TTGTGAACCT CCATCCGTGC CAGTTCTGTC ATGTAAGCTT 
TCTTCTGAGC AAAAAGATGA AGGTACATTG ATGGAATTGT CTAGCTCTTT ACATAGTGAA 
ACAACTGAGG AGGAAGAATG TGGAAATAAA ATGGCTCTTG GAAGTTCAAC ATTGGATGGG 
GAAATATCTG ACTATAAGAA ACTAAACAGT CAAATTAAGC CTTTGCCACA AGCAATAGAA 
AGCTTGGTCA TGGACACGTG TGATAGCAAT CTGTGCAATA ATATTAATTT AAAAGATAGC 
AGCAAAATAA TTTCAGAGGA AGCTACAGTA CCACATTGTT TTCAGCCAGA ACAGCATGTT 
GTATACAGCC ATCCTATGTC TGACCCTGCA GTTACTTGTG TGACTCATGG CACTGGATCT 
GAAATGAAAG CATTGGATAC ACCAAGGAAG GAGTCTAATC CAGTGTGTAA GGAACTCCGT 
GAAAGAAATG TAAATCCAAT GAAAGAATTG TATATATCTC TGAACAATGC ACCAACTGAT 
TTGTCTAACT CTTTGTGCAG TGACACTACT AATGTGGAAG AATCTAGGGA TGCCACAGCA 
AATGCAAGTT CTTTATCCAA CGGAGAAATA TTGCATTTAT ATCACAAAAC AAGCAGCCCA 
GCTCTTGTTG ATCCAAAAGC AGAAGCAAAT ACAGTTTTAG GCTTGTGTGA CAGTGTCCTC 
AGCAAAAACT CTACAATGGG AAGTAGTGAT AGCATTAATC AGGAGAAAGC TATAGCAACA 
ACTTTTGCAC ACCCAGGACA GAGTGTGTTG TTCAGCCATG GGGCATCTAC AATTACAGTC 
TCCCCTGGTT TATTTCATAG TGCTGTACAG GCATTAGATG TTTCTGATAC ACCAGAGTTG 
GGGTGCAGTC CTGTGTGTAA GGAACCCACT GTTGTACAAG ATAAAAGCGA TGGATCCTTT 
GATACTTCTT TGGGCCATTT TGAAAGAGAA AAGCAAAATG AGAGCTTTGA AACAGACAGT 
TCTGTAATGC TTACTGATGA TCACATAAAG GACCCAAAGG ATGTAGATGT TGTATTGTCT 
TTGCCAGAGG GGAGCTTGGA AGATTGCTGT CCCAAAAAAA AATCAAACTG CTCAGAGAAC 
ATGCATGTAG CCTTAAAGGG TACCTCCACA AACCCTTCCC TTGAACTCGG TTCCCACGAT 
GCCCCGTGTG AAAAGAAGCT GCTTCAATGG AGAAACACCC TCTTCCTCTG CTGGTTGGAC 
TGCATATTGG CAGCACTGGT GCATTCTGCT AGCCTGAATA GCATTGTGGC ACAGCGAAGG 
GTTGCAGAAA GCTCAATCAT TCACCTTCTT CTGGAGAAAT ACAAGGAGGC CAATGCATTA 
ATAAAAAGCA CATCCAGACG AGGACGTCCA AAGAATTTAT CTCAAGCTGA AAACATCCTG 
AATAAAGTGC GAATGACCAT CTTTGATGAA ATAAAGCCCT TGTTAAAGTG TGAATTGGGA 
GATAAAGAGA GCCCTGTGTT TGCTTTGCCG TTACTGCTGC AGCTGGTTCC AGAGATAGAA 
TCCTGTTTTG TGCACTCAGG TGTATGGGAG TTTACATGCA AGCTATGTGG CTACCAATAC 
CAAAATAAGC ACCAACGGAC TGTGTCCACA TTCACTAAGA TCTTACCTGA ATGGACACCC 
CTAAATGCTA TCCATAAAGC TCCTTGCAAC AAATGCCTGG GTACAGATCA GATACGTGCT 
TTTCGTATTA AAAAGATCCA TGACCTATTC ATACTTCACT TTGTGGAAGG CCTGCCAAGC 
AATGATTTAG GCATGTACTC GTTTCACTTT GAAGGCTGCC TGTATGAAGT AAAGACTGTC 
ATCCAGTATA GTGAAGACCA TTTCTCATCT TGGATTGCTA ATGAGGATGG CACATGGCTG 
GAATCTGATG ACCTGAAAGG GTATTTCTGC ATAAAACAGA ACTGTTTTGA GGTTCCTGCT 
TCAGAAATTC ATATTGTAAT ATGGGAAAAG AAGATTACCA ACAGCACTGG AGCACACCAT 
GCAATGGAAC CTGAAACAAA AAACAAAGCA CTTAATTCTT TATCTACTTC ACCTTCATCT 
TTATCTCAGA CCCTGCACCT GTCTCCTTCA GCTGCTCCGA ATGCTGCATC TTCACCTCTA 
AGAGATGCTC CAAATGCTTC CAGCGTACTC TCTGGAATGG AGGGTTTTCA TGATGACGAC 
ATCATAACGC TCACTCTCAT TGAAATTCCA ATTGATGCAT CGGGTAATGT GATTGAGAAC 
AACTCTGAGG CAGCCCAGCA GCCAAGTCTC ATTGCTGAAA CACATTCATC CCCTGTAACC 
CTCAAAGCAG TTGCTGATGG GCAATGTGAT CAAAATCTAT GTTCTGGTAC AATGAAGGAA 
AGCTCTTTCG CTGGTGGTTC TCTTCCAGTT CCTAACATTT CCAGCCCTCC TGCCCCTAAC 
ATTTCCAGCC CTCCTGCCCC TAACATTTCC AGCCCTCCTG CCCCTCACAT TTCCAGCCCT 
CCTGCCCCTC ACATTTCCAG CCCTCCTGCC CCTCACATTT CCAGCCCTCC TGCCCCTCAC 
ATTTCCAGCC CTCCTGCCCC TCACATTTCC AGCCCTCCTG CCCCTCACAT TTCCAGCCCT 
CCTGCCCCTA ACAGCGCCAG TCCCCCTGCC CCTAACAGCG CCAGTACTCC CCTCCCTAAC 
AGTTCCAGTT CAAAAAAAGC CTTTGTTGGA AGTTGGATGA AGAGTCTACT AAATAAAAAC 
CCTTTGTTCC TGACATCTAA TGTGCCCTGT GCAAATAAGA AAACTGTGGC AAAAACTCCA 
ATCCGATTTC CCCTGCTGAA GACAACAGAT TTGCAGGCAT CTACTAAACA GGCCCAGAGC 
TTTGATGGTT TTAAAGCTAA ACGTGTCGGT AATTCATTCA GCTCGGACAA TTGCCTTGGT 
GTAGGTCCCA CGAAGGATAA TTCCACTGCA CCCAAACCTG CCATCTCTAG CCAAACGTTC 
CAGCCACTGG TTTCAAATCG ACTTAAAAAT GGGCGGTTAG GACTCGGTAA TACCAAAGAC 
TTGGCAAAAA ATGTTTCTCT GTCTAAGGAG GATAAGGTGC TTAGGCTTAG ACTGAAAATG 
CTGAAAAAGT TGAAGGCTAA GAAACATGAA TTGGCCGCCA TAGATATGCT TGGAAAGTCA 
CAGGCTGGTA GTGATCTGGA GCGTAGCAGC ACCAGCAGGC GTGAACAATT ACGCGGGTTC 
TTGCAGGAAC TCCAAGAGCA AATCGATAAT GCCGACACTG AATCAGTGTG CACTATGAGC 
TCTTGCACGT CGATATGCAG CAGCCCAGGA GATGCGGAAT TCTTTGCGGA ATTGTTCTCC 
CCACCCCCTA GCAACGCCCC GCAGGAGAGC ATGGATGACA GTAATTTTTT AGAGATGTAT 
GTAGATGGTT ACAACCCCTC CTGTGGCAGT TATGGAACCA GTAACCCAAG TGGCAAAGAG 
GTCTCACAGG ACAGTGCTAA TATCGGCCAG TCCAATTTAC AGCGTGCTTC CGACAGCCAC 
AATAACACTA ACGTGGGGGA CGTCTTCCAT GATATGCTGT CAACCTCCAC AATGCACATG 
CTGAACGAGG ATTATTTCTC ACCTTTTGAT GATATGTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

uspl1 ( XM_018091731.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947220.2 Xenopus tropicalis ubiquitin specific peptidase like 1 (uspl1), transcript variant X2, mRNA.

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CloneID	OXa52820
Clone ID Related Accession (Same CDS sequence)	XM_018091733.2 , XM_018091731.2
Accession Version	XM_018091731.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	SUMO-specific isopeptidase USPL1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018091731.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTAAAG TAAGCCAATG GTTCACAACG ATGGCTTCAG AGACTCCTGG ATCTACAGTG 
TCACTGGTTG GACAAGGGTC TATTTTAGAT AAATCTTCAC TCCACACGGT GGGGTGCTTG 
GGAAAAAATG TTGACCCCTG TGCAGCTCCT GCTGATGGAT TTTGCCCAGT ATGCAAGGCC 
AAAGGCCAGC TGCAAGCTCT CCGGACATAT CGCTTTAATT TCACTCAGTC TATATATCTC 
TGTGCCAATC CACAGTGCAT ATATCCCTTG GGATATACAC CTCTGGATAA CATAATTGCA 
AACACAGCAG ATCTACAGAA ACACCATAGC CCAGGAAAGC AGAAGAAGCG GTGTCTTTTA 
GACACCTCTG TAACAACTAA CTGTGTGAAG AAGCATAAGA CTGATTCTTT TGTCTCTGGA 
GAGTACATTT CAGAAAACCT ATTTTTATCT GCACAAAATA AAGGCTACAA ATCAATGTCT 
CCGTGTTTAC CTAAAGAATC TCATACAAGT GTACCTGGCC CTTCAGAGAA TGGTCAGGAT 
GAGAGAAGTC AGCAGCAATA TGGAATGTGC AAAGTTTCAG CTTGCAATTC TGCAAGTCCA 
CATGGTGACA GGGCACCTTC AGTTGAATTT GCTCTGCGGG ACTCAAACCA ACCTAATCAC 
CCGCAGGAAG CCTTAAATGC ATTTCACGAA GAAGCTGTGC AAAATCAAGT TATGGGAATG 
ACCATTGCAT CAATTAATGG TCCTTCTCCT TTAAAATGGA ATGTACCTGC TACTAAAGAT 
ATGCACACAT TATCACAGTC GGTGAATGCT GCTAATGACC CACCAACATG TATAAACCAA 
AAGACAGATG CTGGAATAGC TGAGGTTTTA AATGGATTTA CAAAGGCTGA AGCCAATATG 
TGCCATTCAG TATCTGTCCT TAGTACTTGT GCACCTTTAG GCTCACCTCG TTTAAGGCTT 
AGTGCTCTAA AGGAGGGACA TAAACAGAGT GATATACGGA GTGCCTCCTG CAGTAGTGCT 
TGTCTTGAAA AAGTAGCATC AGAAACAGCT CTTGCCGCCA TGTCTGACAG CAACATGAAG 
AAAGAGGATA GTTTCAACAT GGCATTTGAA CTTTTACCGT CGTCTCCTCC ATTGCATTCA 
TTTTTTGGGG CTTCACAAAA TGTGCACACA TCTGAAGAAT CATCCTCTTT GCTTGGAACA 
GTGGGTCAAA CAAAAGAAAA TACCATAAGT GTTAAATCAA TTGGATCCAG CAATGTTGTT 
CTTAATGAAG GAAAGGGGGA AACCTCCCAG TTTACGATAT TGTCCAAATC ACACAGTGAG 
GTAGAAGATG TTCAGAAAGT GTGTGTACGT TTGCCCTCTT CCCCACTGCT TGAAACAGCT 
TTACAAATGA AAGCGTATGC CCACAGTGAC AAGCTCAGTG CCTTCAACAA TAATCCATGT 
CTTGAGAAAG TATTACCAGA GGCAACCCCA GTGGTTTTAT CAGAAACATA TAGGGAGGTA 
CAAGATTGTT TTGGATCTTT ACCTTCATCT GATTTGCATG AAAATGAGCC TCCACTGGAA 
GAGAGATCCC TGGTTGATAA ATCTAATAGC TCCAGAAATT ATACTTGCCT TGAGAAAGTA 
GTACCAGAGG CAACCTTATT GTTAAAAGAG TACTTTTCAG AATCCTGTAG TAAGGTACAA 
GATGATGAGA AATGTTTTGG ATCTTTACCT TCATCTGCTT TGCAAAGAAA AAATCCTCTA 
ATTGAAAAAA ACGGACCGAG TGACAAATCC AGTGCATCCA GCAATAATGT TTGCCTTGAG 
GAAGGAGAAC CAGAGGCAGT CCCATTGTTG GAAGACTGTA AAAGTCAAAC CCTTGTTGTA 
CCTTCCACAT GCAGTCAATT GTCCATAACA GATAAAATAG CAAACAGTCT TGGAGTACAT 
AATTCCTCAG ATTCATGTCA GCTCATCAGT GAAATTCATC ATGATCATCC AAGCGATGGA 
AATGATCTCA CTGTGTCCCA TATTGCCTTC AATGTTGGTG GTGCTGACAA AATCAATAAA 
GTTAAAAGCC TGCAGGTAAC TCCACTTCCA GAGGTTATAT CTACAGAACT ATGTAGGGGC 
AAGAGCAACA GTCAAAGCAC TTGTGAACCT CCATCCGTGC CAGTTCTGTC ATGTAAGCTT 
TCTTCTGAGC AAAAAGATGA AGGTACATTG ATGGAATTGT CTAGCTCTTT ACATAGTGAA 
ACAACTGAGG AGGAAGAATG TGGAAATAAA ATGGCTCTTG GAAGTTCAAC ATTGGATGGG 
GAAATATCTG ACTATAAGAA ACTAAACAGT CAAATTAAGC CTTTGCCACA AGCAATAGAA 
AGCTTGGTCA TGGACACGTG TGATAGCAAT CTGTGCAATA ATATTAATTT AAAAGATAGC 
AGCAAAATAA TTTCAGAGGA AGCTACAGTA CCACATTGTT TTCAGCCAGA ACAGCATGTT 
GTATACAGCC ATCCTATGTC TGACCCTGCA GTTACTTGTG TGACTCATGG CACTGGATCT 
GAAATGAAAG CATTGGATAC ACCAAGGAAG GAGTCTAATC CAGTGTGTAA GGAACTCCGT 
GAAAGAAATG TAAATCCAAT GAAAGAATTG TATATATCTC TGAACAATGC ACCAACTGAT 
TTGTCTAACT CTTTGTGCAG TGACACTACT AATGTGGAAG AATCTAGGGA TGCCACAGCA 
AATGCAAGTT CTTTATCCAA CGGAGAAATA TTGCATTTAT ATCACAAAAC AAGCAGCCCA 
GCTCTTGTTG ATCCAAAAGC AGAAGCAAAT ACAGTTTTAG GCTTGTGTGA CAGTGTCCTC 
AGCAAAAACT CTACAATGGG AAGTAGTGAT AGCATTAATC AGGAGAAAGC TATAGCAACA 
ACTTTTGCAC ACCCAGGACA GAGTGTGTTG TTCAGCCATG GGGCATCTAC AATTACAGTC 
TCCCCTGGTT TATTTCATAG TGCTGTACAG GCATTAGATG TTTCTGATAC ACCAGAGTTG 
GGGTGCAGTC CTGTGTGTAA GGAACCCACT GTTGTACAAG ATAAAAGCGA TGGATCCTTT 
GATACTTCTT TGGGCCATTT TGAAAGAGAA AAGCAAAATG AGAGCTTTGA AACAGACAGT 
TCTGTAATGC TTACTGATGA TCACATAAAG GACCCAAAGG ATGTAGATGT TGTATTGTCT 
TTGCCAGAGG GGAGCTTGGA AGATTGCTGT CCCAAAAAAA AATCAAACTG CTCAGAGAAC 
ATGCATGTAG CCTTAAAGGG TACCTCCACA AACCCTTCCC TTGAACTCGG TTCCCACGAT 
GCCCCGTGTG AAAAGAAGCT GCTTCAATGG AGAAACACCC TCTTCCTCTG CTGGTTGGAC 
TGCATATTGG CAGCACTGGT GCATTCTGCT AGCCTGAATA GCATTGTGGC ACAGCGAAGG 
GTTGCAGAAA GCTCAATCAT TCACCTTCTT CTGGAGAAAT ACAAGGAGGC CAATGCATTA 
ATAAAAAGCA CATCCAGACG AGGACGTCCA AAGAATTTAT CTCAAGCTGA AAACATCCTG 
AATAAAGTGC GAATGACCAT CTTTGATGAA ATAAAGCCCT TGTTAAAGTG TGAATTGGGA 
GATAAAGAGA GCCCTGTGTT TGCTTTGCCG TTACTGCTGC AGCTGGTTCC AGAGATAGAA 
TCCTGTTTTG TGCACTCAGG TGTATGGGAG TTTACATGCA AGCTATGTGG CTACCAATAC 
CAAAATAAGC ACCAACGGAC TGTGTCCACA TTCACTAAGA TCTTACCTGA ATGGACACCC 
CTAAATGCTA TCCATAAAGC TCCTTGCAAC AAATGCCTGG GTACAGATCA GATACGTGCT 
TTTCGTATTA AAAAGATCCA TGACCTATTC ATACTTCACT TTGTGGAAGG CCTGCCAAGC 
AATGATTTAG GCATGTACTC GTTTCACTTT GAAGGCTGCC TGTATGAAGT AAAGACTGTC 
ATCCAGTATA GTGAAGACCA TTTCTCATCT TGGATTGCTA ATGAGGATGG CACATGGCTG 
GAATCTGATG ACCTGAAAGG GTATTTCTGC ATAAAACAGA ACTGTTTTGA GGTTCCTGCT 
TCAGAAATTC ATATTGTAAT ATGGGAAAAG AAGATTACCA ACAGCACTGG AGCACACCAT 
GCAATGGAAC CTGAAACAAA AAACAAAGCA CTTAATTCTT TATCTACTTC ACCTTCATCT 
TTATCTCAGA CCCTGCACCT GTCTCCTTCA GCTGCTCCGA ATGCTGCATC TTCACCTCTA 
AGAGATGCTC CAAATGCTTC CAGCGTACTC TCTGGAATGG AGGGTTTTCA TGATGACGAC 
ATCATAACGC TCACTCTCAT TGAAATTCCA ATTGATGCAT CGGGTAATGT GATTGAGAAC 
AACTCTGAGG CAGCCCAGCA GCCAAGTCTC ATTGCTGAAA CACATTCATC CCCTGTAACC 
CTCAAAGCAG TTGCTGATGG GCAATGTGAT CAAAATCTAT GTTCTGGTAC AATGAAGGAA 
AGCTCTTTCG CTGGTGGTTC TCTTCCAGTT CCTAACATTT CCAGCCCTCC TGCCCCTAAC 
ATTTCCAGCC CTCCTGCCCC TAACATTTCC AGCCCTCCTG CCCCTCACAT TTCCAGCCCT 
CCTGCCCCTC ACATTTCCAG CCCTCCTGCC CCTCACATTT CCAGCCCTCC TGCCCCTCAC 
ATTTCCAGCC CTCCTGCCCC TCACATTTCC AGCCCTCCTG CCCCTCACAT TTCCAGCCCT 
CCTGCCCCTA ACAGCGCCAG TCCCCCTGCC CCTAACAGCG CCAGTACTCC CCTCCCTAAC 
AGTTCCAGTT CAAAAAAAGC CTTTGTTGGA AGTTGGATGA AGAGTCTACT AAATAAAAAC 
CCTTTGTTCC TGACATCTAA TGTGCCCTGT GCAAATAAGA AAACTGTGGC AAAAACTCCA 
ATCCGATTTC CCCTGCTGAA GACAACAGAT TTGCAGGCAT CTACTAAACA GGCCCAGAGC 
TTTGATGGTT TTAAAGCTAA ACGTGTCGGT AATTCATTCA GCTCGGACAA TTGCCTTGGT 
GTAGGTCCCA CGAAGGATAA TTCCACTGCA CCCAAACCTG CCATCTCTAG CCAAACGTTC 
CAGCCACTGG TTTCAAATCG ACTTAAAAAT GGGCGGTTAG GACTCGGTAA TACCAAAGAC 
TTGGCAAAAA ATGTTTCTCT GTCTAAGGAG GATAAGGTGC TTAGGCTTAG ACTGAAAATG 
CTGAAAAAGT TGAAGGCTAA GAAACATGAA TTGGCCGCCA TAGATATGCT TGGAAAGTCA 
CAGGCTGGTA GTGATCTGGA GCGTAGCAGC ACCAGCAGGC GTGAACAATT ACGCGGGTTC 
TTGCAGGAAC TCCAAGAGCA AATCGATAAT GCCGACACTG AATCAGTGTG CACTATGAGC 
TCTTGCACGT CGATATGCAG CAGCCCAGGA GATGCGGAAT TCTTTGCGGA ATTGTTCTCC 
CCACCCCCTA GCAACGCCCC GCAGGAGAGC ATGGATGACA GTAATTTTTT AGAGATGTAT 
GTAGATGGTT ACAACCCCTC CTGTGGCAGT TATGGAACCA GTAACCCAAG TGGCAAAGAG 
GTCTCACAGG ACAGTGCTAA TATCGGCCAG TCCAATTTAC AGCGTGCTTC CGACAGCCAC 
AATAACACTA ACGTGGGGGA CGTCTTCCAT GATATGCTGT CAACCTCCAC AATGCACATG 
CTGAACGAGG ATTATTTCTC ACCTTTTGAT GATATGTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947220.2
CDS	2792..8533
Translation	MTKVSQWFTTMASETPGSTVSLVGQGSILDKSSLHTVGCLGKNVDPCAAPADGFCPVCKAKGQLQALRTYRFNFTQSIYLCANPQCIYPLGYTPLDNIIANTADLQKHHSPGKQKKRCLLDTSVTTNCVKKHKTDSFVSGEYISENLFLSAQNKGYKSMSPCLPKESHTSVPGPSENGQDERSQQQYGMCKVSACNSASPHGDRAPSVEFALRDSNQPNHPQEALNAFHEEAVQNQVMGMTIASINGPSPLKWNVPATKDMHTLSQSVNAANDPPTCINQKTDAGIAEVLNGFTKAEANMCHSVSVLSTCAPLGSPRLRLSALKEGHKQSDIRSASCSSACLEKVASETALAAMSDSNMKKEDSFNMAFELLPSSPPLHSFFGASQNVHTSEESSSLLGTVGQTKENTISVKSIGSSNVVLNEGKGETSQFTILSKSHSEVEDVQKVCVRLPSSPLLETALQMKAYAHSDKLSAFNNNPCLEKVLPEATPVVLSETYREVQDCFGSLPSSDLHENEPPLEERSLVDKSNSSRNYTCLEKVVPEATLLLKEYFSESCSKVQDDEKCFGSLPSSALQRKNPLIEKNGPSDKSSASSNNVCLEEGEPEAVPLLEDCKSQTLVVPSTCSQLSITDKIANSLGVHNSSDSCQLISEIHHDHPSDGNDLTVSHIAFNVGGADKINKVKSLQVTPLPEVISTELCRGKSNSQSTCEPPSVPVLSCKLSSEQKDEGTLMELSSSLHSETTEEEECGNKMALGSSTLDGEISDYKKLNSQIKPLPQAIESLVMDTCDSNLCNNINLKDSSKIISEEATVPHCFQPEQHVVYSHPMSDPAVTCVTHGTGSEMKALDTPRKESNPVCKELRERNVNPMKELYISLNNAPTDLSNSLCSDTTNVEESRDATANASSLSNGEILHLYHKTSSPALVDPKAEANTVLGLCDSVLSKNSTMGSSDSINQEKAIATTFAHPGQSVLFSHGASTITVSPGLFHSAVQALDVSDTPELGCSPVCKEPTVVQDKSDGSFDTSLGHFEREKQNESFETDSSVMLTDDHIKDPKDVDVVLSLPEGSLEDCCPKKKSNCSENMHVALKGTSTNPSLELGSHDAPCEKKLLQWRNTLFLCWLDCILAALVHSASLNSIVAQRRVAESSIIHLLLEKYKEANALIKSTSRRGRPKNLSQAENILNKVRMTIFDEIKPLLKCELGDKESPVFALPLLLQLVPEIESCFVHSGVWEFTCKLCGYQYQNKHQRTVSTFTKILPEWTPLNAIHKAPCNKCLGTDQIRAFRIKKIHDLFILHFVEGLPSNDLGMYSFHFEGCLYEVKTVIQYSEDHFSSWIANEDGTWLESDDLKGYFCIKQNCFEVPASEIHIVIWEKKITNSTGAHHAMEPETKNKALNSLSTSPSSLSQTLHLSPSAAPNAASSPLRDAPNASSVLSGMEGFHDDDIITLTLIEIPIDASGNVIENNSEAAQQPSLIAETHSSPVTLKAVADGQCDQNLCSGTMKESSFAGGSLPVPNISSPPAPNISSPPAPNISSPPAPHISSPPAPHISSPPAPHISSPPAPHISSPPAPHISSPPAPHISSPPAPNSASPPAPNSASTPLPNSSSSKKAFVGSWMKSLLNKNPLFLTSNVPCANKKTVAKTPIRFPLLKTTDLQASTKQAQSFDGFKAKRVGNSFSSDNCLGVGPTKDNSTAPKPAISSQTFQPLVSNRLKNGRLGLGNTKDLAKNVSLSKEDKVLRLRLKMLKKLKAKKHELAAIDMLGKSQAGSDLERSSTSRREQLRGFLQELQEQIDNADTESVCTMSSCTSICSSPGDAEFFAELFSPPPSNAPQESMDDSNFLEMYVDGYNPSCGSYGTSNPSGKEVSQDSANIGQSNLQRASDSHNNTNVGDVFHDMLSTSTMHMLNEDYFSPFDDMF

Target ORF information:

RefSeq Version	XM_018091731.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin specific peptidase like 1 (uspl1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018091731.2

ORF Insert Sequence:

ATGACTAAAG TAAGCCAATG GTTCACAACG ATGGCTTCAG AGACTCCTGG ATCTACAGTG 
TCACTGGTTG GACAAGGGTC TATTTTAGAT AAATCTTCAC TCCACACGGT GGGGTGCTTG 
GGAAAAAATG TTGACCCCTG TGCAGCTCCT GCTGATGGAT TTTGCCCAGT ATGCAAGGCC 
AAAGGCCAGC TGCAAGCTCT CCGGACATAT CGCTTTAATT TCACTCAGTC TATATATCTC 
TGTGCCAATC CACAGTGCAT ATATCCCTTG GGATATACAC CTCTGGATAA CATAATTGCA 
AACACAGCAG ATCTACAGAA ACACCATAGC CCAGGAAAGC AGAAGAAGCG GTGTCTTTTA 
GACACCTCTG TAACAACTAA CTGTGTGAAG AAGCATAAGA CTGATTCTTT TGTCTCTGGA 
GAGTACATTT CAGAAAACCT ATTTTTATCT GCACAAAATA AAGGCTACAA ATCAATGTCT 
CCGTGTTTAC CTAAAGAATC TCATACAAGT GTACCTGGCC CTTCAGAGAA TGGTCAGGAT 
GAGAGAAGTC AGCAGCAATA TGGAATGTGC AAAGTTTCAG CTTGCAATTC TGCAAGTCCA 
CATGGTGACA GGGCACCTTC AGTTGAATTT GCTCTGCGGG ACTCAAACCA ACCTAATCAC 
CCGCAGGAAG CCTTAAATGC ATTTCACGAA GAAGCTGTGC AAAATCAAGT TATGGGAATG 
ACCATTGCAT CAATTAATGG TCCTTCTCCT TTAAAATGGA ATGTACCTGC TACTAAAGAT 
ATGCACACAT TATCACAGTC GGTGAATGCT GCTAATGACC CACCAACATG TATAAACCAA 
AAGACAGATG CTGGAATAGC TGAGGTTTTA AATGGATTTA CAAAGGCTGA AGCCAATATG 
TGCCATTCAG TATCTGTCCT TAGTACTTGT GCACCTTTAG GCTCACCTCG TTTAAGGCTT 
AGTGCTCTAA AGGAGGGACA TAAACAGAGT GATATACGGA GTGCCTCCTG CAGTAGTGCT 
TGTCTTGAAA AAGTAGCATC AGAAACAGCT CTTGCCGCCA TGTCTGACAG CAACATGAAG 
AAAGAGGATA GTTTCAACAT GGCATTTGAA CTTTTACCGT CGTCTCCTCC ATTGCATTCA 
TTTTTTGGGG CTTCACAAAA TGTGCACACA TCTGAAGAAT CATCCTCTTT GCTTGGAACA 
GTGGGTCAAA CAAAAGAAAA TACCATAAGT GTTAAATCAA TTGGATCCAG CAATGTTGTT 
CTTAATGAAG GAAAGGGGGA AACCTCCCAG TTTACGATAT TGTCCAAATC ACACAGTGAG 
GTAGAAGATG TTCAGAAAGT GTGTGTACGT TTGCCCTCTT CCCCACTGCT TGAAACAGCT 
TTACAAATGA AAGCGTATGC CCACAGTGAC AAGCTCAGTG CCTTCAACAA TAATCCATGT 
CTTGAGAAAG TATTACCAGA GGCAACCCCA GTGGTTTTAT CAGAAACATA TAGGGAGGTA 
CAAGATTGTT TTGGATCTTT ACCTTCATCT GATTTGCATG AAAATGAGCC TCCACTGGAA 
GAGAGATCCC TGGTTGATAA ATCTAATAGC TCCAGAAATT ATACTTGCCT TGAGAAAGTA 
GTACCAGAGG CAACCTTATT GTTAAAAGAG TACTTTTCAG AATCCTGTAG TAAGGTACAA 
GATGATGAGA AATGTTTTGG ATCTTTACCT TCATCTGCTT TGCAAAGAAA AAATCCTCTA 
ATTGAAAAAA ACGGACCGAG TGACAAATCC AGTGCATCCA GCAATAATGT TTGCCTTGAG 
GAAGGAGAAC CAGAGGCAGT CCCATTGTTG GAAGACTGTA AAAGTCAAAC CCTTGTTGTA 
CCTTCCACAT GCAGTCAATT GTCCATAACA GATAAAATAG CAAACAGTCT TGGAGTACAT 
AATTCCTCAG ATTCATGTCA GCTCATCAGT GAAATTCATC ATGATCATCC AAGCGATGGA 
AATGATCTCA CTGTGTCCCA TATTGCCTTC AATGTTGGTG GTGCTGACAA AATCAATAAA 
GTTAAAAGCC TGCAGGTAAC TCCACTTCCA GAGGTTATAT CTACAGAACT ATGTAGGGGC 
AAGAGCAACA GTCAAAGCAC TTGTGAACCT CCATCCGTGC CAGTTCTGTC ATGTAAGCTT 
TCTTCTGAGC AAAAAGATGA AGGTACATTG ATGGAATTGT CTAGCTCTTT ACATAGTGAA 
ACAACTGAGG AGGAAGAATG TGGAAATAAA ATGGCTCTTG GAAGTTCAAC ATTGGATGGG 
GAAATATCTG ACTATAAGAA ACTAAACAGT CAAATTAAGC CTTTGCCACA AGCAATAGAA 
AGCTTGGTCA TGGACACGTG TGATAGCAAT CTGTGCAATA ATATTAATTT AAAAGATAGC 
AGCAAAATAA TTTCAGAGGA AGCTACAGTA CCACATTGTT TTCAGCCAGA ACAGCATGTT 
GTATACAGCC ATCCTATGTC TGACCCTGCA GTTACTTGTG TGACTCATGG CACTGGATCT 
GAAATGAAAG CATTGGATAC ACCAAGGAAG GAGTCTAATC CAGTGTGTAA GGAACTCCGT 
GAAAGAAATG TAAATCCAAT GAAAGAATTG TATATATCTC TGAACAATGC ACCAACTGAT 
TTGTCTAACT CTTTGTGCAG TGACACTACT AATGTGGAAG AATCTAGGGA TGCCACAGCA 
AATGCAAGTT CTTTATCCAA CGGAGAAATA TTGCATTTAT ATCACAAAAC AAGCAGCCCA 
GCTCTTGTTG ATCCAAAAGC AGAAGCAAAT ACAGTTTTAG GCTTGTGTGA CAGTGTCCTC 
AGCAAAAACT CTACAATGGG AAGTAGTGAT AGCATTAATC AGGAGAAAGC TATAGCAACA 
ACTTTTGCAC ACCCAGGACA GAGTGTGTTG TTCAGCCATG GGGCATCTAC AATTACAGTC 
TCCCCTGGTT TATTTCATAG TGCTGTACAG GCATTAGATG TTTCTGATAC ACCAGAGTTG 
GGGTGCAGTC CTGTGTGTAA GGAACCCACT GTTGTACAAG ATAAAAGCGA TGGATCCTTT 
GATACTTCTT TGGGCCATTT TGAAAGAGAA AAGCAAAATG AGAGCTTTGA AACAGACAGT 
TCTGTAATGC TTACTGATGA TCACATAAAG GACCCAAAGG ATGTAGATGT TGTATTGTCT 
TTGCCAGAGG GGAGCTTGGA AGATTGCTGT CCCAAAAAAA AATCAAACTG CTCAGAGAAC 
ATGCATGTAG CCTTAAAGGG TACCTCCACA AACCCTTCCC TTGAACTCGG TTCCCACGAT 
GCCCCGTGTG AAAAGAAGCT GCTTCAATGG AGAAACACCC TCTTCCTCTG CTGGTTGGAC 
TGCATATTGG CAGCACTGGT GCATTCTGCT AGCCTGAATA GCATTGTGGC ACAGCGAAGG 
GTTGCAGAAA GCTCAATCAT TCACCTTCTT CTGGAGAAAT ACAAGGAGGC CAATGCATTA 
ATAAAAAGCA CATCCAGACG AGGACGTCCA AAGAATTTAT CTCAAGCTGA AAACATCCTG 
AATAAAGTGC GAATGACCAT CTTTGATGAA ATAAAGCCCT TGTTAAAGTG TGAATTGGGA 
GATAAAGAGA GCCCTGTGTT TGCTTTGCCG TTACTGCTGC AGCTGGTTCC AGAGATAGAA 
TCCTGTTTTG TGCACTCAGG TGTATGGGAG TTTACATGCA AGCTATGTGG CTACCAATAC 
CAAAATAAGC ACCAACGGAC TGTGTCCACA TTCACTAAGA TCTTACCTGA ATGGACACCC 
CTAAATGCTA TCCATAAAGC TCCTTGCAAC AAATGCCTGG GTACAGATCA GATACGTGCT 
TTTCGTATTA AAAAGATCCA TGACCTATTC ATACTTCACT TTGTGGAAGG CCTGCCAAGC 
AATGATTTAG GCATGTACTC GTTTCACTTT GAAGGCTGCC TGTATGAAGT AAAGACTGTC 
ATCCAGTATA GTGAAGACCA TTTCTCATCT TGGATTGCTA ATGAGGATGG CACATGGCTG 
GAATCTGATG ACCTGAAAGG GTATTTCTGC ATAAAACAGA ACTGTTTTGA GGTTCCTGCT 
TCAGAAATTC ATATTGTAAT ATGGGAAAAG AAGATTACCA ACAGCACTGG AGCACACCAT 
GCAATGGAAC CTGAAACAAA AAACAAAGCA CTTAATTCTT TATCTACTTC ACCTTCATCT 
TTATCTCAGA CCCTGCACCT GTCTCCTTCA GCTGCTCCGA ATGCTGCATC TTCACCTCTA 
AGAGATGCTC CAAATGCTTC CAGCGTACTC TCTGGAATGG AGGGTTTTCA TGATGACGAC 
ATCATAACGC TCACTCTCAT TGAAATTCCA ATTGATGCAT CGGGTAATGT GATTGAGAAC 
AACTCTGAGG CAGCCCAGCA GCCAAGTCTC ATTGCTGAAA CACATTCATC CCCTGTAACC 
CTCAAAGCAG TTGCTGATGG GCAATGTGAT CAAAATCTAT GTTCTGGTAC AATGAAGGAA 
AGCTCTTTCG CTGGTGGTTC TCTTCCAGTT CCTAACATTT CCAGCCCTCC TGCCCCTAAC 
ATTTCCAGCC CTCCTGCCCC TAACATTTCC AGCCCTCCTG CCCCTCACAT TTCCAGCCCT 
CCTGCCCCTC ACATTTCCAG CCCTCCTGCC CCTCACATTT CCAGCCCTCC TGCCCCTCAC 
ATTTCCAGCC CTCCTGCCCC TCACATTTCC AGCCCTCCTG CCCCTCACAT TTCCAGCCCT 
CCTGCCCCTA ACAGCGCCAG TCCCCCTGCC CCTAACAGCG CCAGTACTCC CCTCCCTAAC 
AGTTCCAGTT CAAAAAAAGC CTTTGTTGGA AGTTGGATGA AGAGTCTACT AAATAAAAAC 
CCTTTGTTCC TGACATCTAA TGTGCCCTGT GCAAATAAGA AAACTGTGGC AAAAACTCCA 
ATCCGATTTC CCCTGCTGAA GACAACAGAT TTGCAGGCAT CTACTAAACA GGCCCAGAGC 
TTTGATGGTT TTAAAGCTAA ACGTGTCGGT AATTCATTCA GCTCGGACAA TTGCCTTGGT 
GTAGGTCCCA CGAAGGATAA TTCCACTGCA CCCAAACCTG CCATCTCTAG CCAAACGTTC 
CAGCCACTGG TTTCAAATCG ACTTAAAAAT GGGCGGTTAG GACTCGGTAA TACCAAAGAC 
TTGGCAAAAA ATGTTTCTCT GTCTAAGGAG GATAAGGTGC TTAGGCTTAG ACTGAAAATG 
CTGAAAAAGT TGAAGGCTAA GAAACATGAA TTGGCCGCCA TAGATATGCT TGGAAAGTCA 
CAGGCTGGTA GTGATCTGGA GCGTAGCAGC ACCAGCAGGC GTGAACAATT ACGCGGGTTC 
TTGCAGGAAC TCCAAGAGCA AATCGATAAT GCCGACACTG AATCAGTGTG CACTATGAGC 
TCTTGCACGT CGATATGCAG CAGCCCAGGA GATGCGGAAT TCTTTGCGGA ATTGTTCTCC 
CCACCCCCTA GCAACGCCCC GCAGGAGAGC ATGGATGACA GTAATTTTTT AGAGATGTAT 
GTAGATGGTT ACAACCCCTC CTGTGGCAGT TATGGAACCA GTAACCCAAG TGGCAAAGAG 
GTCTCACAGG ACAGTGCTAA TATCGGCCAG TCCAATTTAC AGCGTGCTTC CGACAGCCAC 
AATAACACTA ACGTGGGGGA CGTCTTCCAT GATATGCTGT CAACCTCCAC AATGCACATG 
CTGAACGAGG ATTATTTCTC ACCTTTTGAT GATATGTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

uspl1 ( XM_018091734.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947223.2 Xenopus tropicalis ubiquitin specific peptidase like 1 (uspl1), transcript variant X3, mRNA.

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CloneID	OXa52821
Clone ID Related Accession (Same CDS sequence)	XM_018091734.2
Accession Version	XM_018091734.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	SUMO-specific isopeptidase USPL1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018091734.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCCGT GTTTACCTAA AGAATCTCAT ACAAGTGTAC CTGGCCCTTC AGAGAATGGT 
CAGGATGAGA GAAGTCAGCA GCAATATGGA ATGTGCAAAG TTTCAGCTTG CAATTCTGCA 
AGTCCACATG GTGACAGGGC ACCTTCAGTT GAATTTGCTC TGCGGGACTC AAACCAACCT 
AATCACCCGC AGGAAGCCTT AAATGCATTT CACGAAGAAG CTGTGCAAAA TCAAGTTATG 
GGAATGACCA TTGCATCAAT TAATGGTCCT TCTCCTTTAA AATGGAATGT ACCTGCTACT 
AAAGATATGC ACACATTATC ACAGTCGGTG AATGCTGCTA ATGACCCACC AACATGTATA 
AACCAAAAGA CAGATGCTGG AATAGCTGAG GTTTTAAATG GATTTACAAA GGCTGAAGCC 
AATATGTGCC ATTCAGTATC TGTCCTTAGT ACTTGTGCAC CTTTAGGCTC ACCTCGTTTA 
AGGCTTAGTG CTCTAAAGGA GGGACATAAA CAGAGTGATA TACGGAGTGC CTCCTGCAGT 
AGTGCTTGTC TTGAAAAAGT AGCATCAGAA ACAGCTCTTG CCGCCATGTC TGACAGCAAC 
ATGAAGAAAG AGGATAGTTT CAACATGGCA TTTGAACTTT TACCGTCGTC TCCTCCATTG 
CATTCATTTT TTGGGGCTTC ACAAAATGTG CACACATCTG AAGAATCATC CTCTTTGCTT 
GGAACAGTGG GTCAAACAAA AGAAAATACC ATAAGTGTTA AATCAATTGG ATCCAGCAAT 
GTTGTTCTTA ATGAAGGAAA GGGGGAAACC TCCCAGTTTA CGATATTGTC CAAATCACAC 
AGTGAGGTAG AAGATGTTCA GAAAGTGTGT GTACGTTTGC CCTCTTCCCC ACTGCTTGAA 
ACAGCTTTAC AAATGAAAGC GTATGCCCAC AGTGACAAGC TCAGTGCCTT CAACAATAAT 
CCATGTCTTG AGAAAGTATT ACCAGAGGCA ACCCCAGTGG TTTTATCAGA AACATATAGG 
GAGGTACAAG ATTGTTTTGG ATCTTTACCT TCATCTGATT TGCATGAAAA TGAGCCTCCA 
CTGGAAGAGA GATCCCTGGT TGATAAATCT AATAGCTCCA GAAATTATAC TTGCCTTGAG 
AAAGTAGTAC CAGAGGCAAC CTTATTGTTA AAAGAGTACT TTTCAGAATC CTGTAGTAAG 
GTACAAGATG ATGAGAAATG TTTTGGATCT TTACCTTCAT CTGCTTTGCA AAGAAAAAAT 
CCTCTAATTG AAAAAAACGG ACCGAGTGAC AAATCCAGTG CATCCAGCAA TAATGTTTGC 
CTTGAGGAAG GAGAACCAGA GGCAGTCCCA TTGTTGGAAG ACTGTAAAAG TCAAACCCTT 
GTTGTACCTT CCACATGCAG TCAATTGTCC ATAACAGATA AAATAGCAAA CAGTCTTGGA 
GTACATAATT CCTCAGATTC ATGTCAGCTC ATCAGTGAAA TTCATCATGA TCATCCAAGC 
GATGGAAATG ATCTCACTGT GTCCCATATT GCCTTCAATG TTGGTGGTGC TGACAAAATC 
AATAAAGTTA AAAGCCTGCA GGTAACTCCA CTTCCAGAGG TTATATCTAC AGAACTATGT 
AGGGGCAAGA GCAACAGTCA AAGCACTTGT GAACCTCCAT CCGTGCCAGT TCTGTCATGT 
AAGCTTTCTT CTGAGCAAAA AGATGAAGGT ACATTGATGG AATTGTCTAG CTCTTTACAT 
AGTGAAACAA CTGAGGAGGA AGAATGTGGA AATAAAATGG CTCTTGGAAG TTCAACATTG 
GATGGGGAAA TATCTGACTA TAAGAAACTA AACAGTCAAA TTAAGCCTTT GCCACAAGCA 
ATAGAAAGCT TGGTCATGGA CACGTGTGAT AGCAATCTGT GCAATAATAT TAATTTAAAA 
GATAGCAGCA AAATAATTTC AGAGGAAGCT ACAGTACCAC ATTGTTTTCA GCCAGAACAG 
CATGTTGTAT ACAGCCATCC TATGTCTGAC CCTGCAGTTA CTTGTGTGAC TCATGGCACT 
GGATCTGAAA TGAAAGCATT GGATACACCA AGGAAGGAGT CTAATCCAGT GTGTAAGGAA 
CTCCGTGAAA GAAATGTAAA TCCAATGAAA GAATTGTATA TATCTCTGAA CAATGCACCA 
ACTGATTTGT CTAACTCTTT GTGCAGTGAC ACTACTAATG TGGAAGAATC TAGGGATGCC 
ACAGCAAATG CAAGTTCTTT ATCCAACGGA GAAATATTGC ATTTATATCA CAAAACAAGC 
AGCCCAGCTC TTGTTGATCC AAAAGCAGAA GCAAATACAG TTTTAGGCTT GTGTGACAGT 
GTCCTCAGCA AAAACTCTAC AATGGGAAGT AGTGATAGCA TTAATCAGGA GAAAGCTATA 
GCAACAACTT TTGCACACCC AGGACAGAGT GTGTTGTTCA GCCATGGGGC ATCTACAATT 
ACAGTCTCCC CTGGTTTATT TCATAGTGCT GTACAGGCAT TAGATGTTTC TGATACACCA 
GAGTTGGGGT GCAGTCCTGT GTGTAAGGAA CCCACTGTTG TACAAGATAA AAGCGATGGA 
TCCTTTGATA CTTCTTTGGG CCATTTTGAA AGAGAAAAGC AAAATGAGAG CTTTGAAACA 
GACAGTTCTG TAATGCTTAC TGATGATCAC ATAAAGGACC CAAAGGATGT AGATGTTGTA 
TTGTCTTTGC CAGAGGGGAG CTTGGAAGAT TGCTGTCCCA AAAAAAAATC AAACTGCTCA 
GAGAACATGC ATGTAGCCTT AAAGGGTACC TCCACAAACC CTTCCCTTGA ACTCGGTTCC 
CACGATGCCC CGTGTGAAAA GAAGCTGCTT CAATGGAGAA ACACCCTCTT CCTCTGCTGG 
TTGGACTGCA TATTGGCAGC ACTGGTGCAT TCTGCTAGCC TGAATAGCAT TGTGGCACAG 
CGAAGGGTTG CAGAAAGCTC AATCATTCAC CTTCTTCTGG AGAAATACAA GGAGGCCAAT 
GCATTAATAA AAAGCACATC CAGACGAGGA CGTCCAAAGA ATTTATCTCA AGCTGAAAAC 
ATCCTGAATA AAGTGCGAAT GACCATCTTT GATGAAATAA AGCCCTTGTT AAAGTGTGAA 
TTGGGAGATA AAGAGAGCCC TGTGTTTGCT TTGCCGTTAC TGCTGCAGCT GGTTCCAGAG 
ATAGAATCCT GTTTTGTGCA CTCAGGTGTA TGGGAGTTTA CATGCAAGCT ATGTGGCTAC 
CAATACCAAA ATAAGCACCA ACGGACTGTG TCCACATTCA CTAAGATCTT ACCTGAATGG 
ACACCCCTAA ATGCTATCCA TAAAGCTCCT TGCAACAAAT GCCTGGGTAC AGATCAGATA 
CGTGCTTTTC GTATTAAAAA GATCCATGAC CTATTCATAC TTCACTTTGT GGAAGGCCTG 
CCAAGCAATG ATTTAGGCAT GTACTCGTTT CACTTTGAAG GCTGCCTGTA TGAAGTAAAG 
ACTGTCATCC AGTATAGTGA AGACCATTTC TCATCTTGGA TTGCTAATGA GGATGGCACA 
TGGCTGGAAT CTGATGACCT GAAAGGGTAT TTCTGCATAA AACAGAACTG TTTTGAGGTT 
CCTGCTTCAG AAATTCATAT TGTAATATGG GAAAAGAAGA TTACCAACAG CACTGGAGCA 
CACCATGCAA TGGAACCTGA AACAAAAAAC AAAGCACTTA ATTCTTTATC TACTTCACCT 
TCATCTTTAT CTCAGACCCT GCACCTGTCT CCTTCAGCTG CTCCGAATGC TGCATCTTCA 
CCTCTAAGAG ATGCTCCAAA TGCTTCCAGC GTACTCTCTG GAATGGAGGG TTTTCATGAT 
GACGACATCA TAACGCTCAC TCTCATTGAA ATTCCAATTG ATGCATCGGG TAATGTGATT 
GAGAACAACT CTGAGGCAGC CCAGCAGCCA AGTCTCATTG CTGAAACACA TTCATCCCCT 
GTAACCCTCA AAGCAGTTGC TGATGGGCAA TGTGATCAAA ATCTATGTTC TGGTACAATG 
AAGGAAAGCT CTTTCGCTGG TGGTTCTCTT CCAGTTCCTA ACATTTCCAG CCCTCCTGCC 
CCTAACATTT CCAGCCCTCC TGCCCCTAAC ATTTCCAGCC CTCCTGCCCC TCACATTTCC 
AGCCCTCCTG CCCCTCACAT TTCCAGCCCT CCTGCCCCTC ACATTTCCAG CCCTCCTGCC 
CCTCACATTT CCAGCCCTCC TGCCCCTCAC ATTTCCAGCC CTCCTGCCCC TCACATTTCC 
AGCCCTCCTG CCCCTAACAG CGCCAGTCCC CCTGCCCCTA ACAGCGCCAG TACTCCCCTC 
CCTAACAGTT CCAGTTCAAA AAAAGCCTTT GTTGGAAGTT GGATGAAGAG TCTACTAAAT 
AAAAACCCTT TGTTCCTGAC ATCTAATGTG CCCTGTGCAA ATAAGAAAAC TGTGGCAAAA 
ACTCCAATCC GATTTCCCCT GCTGAAGACA ACAGATTTGC AGGCATCTAC TAAACAGGCC 
CAGAGCTTTG ATGGTTTTAA AGCTAAACGT GTCGGTAATT CATTCAGCTC GGACAATTGC 
CTTGGTGTAG GTCCCACGAA GGATAATTCC ACTGCACCCA AACCTGCCAT CTCTAGCCAA 
ACGTTCCAGC CACTGGTTTC AAATCGACTT AAAAATGGGC GGTTAGGACT CGGTAATACC 
AAAGACTTGG CAAAAAATGT TTCTCTGTCT AAGGAGGATA AGGTGCTTAG GCTTAGACTG 
AAAATGCTGA AAAAGTTGAA GGCTAAGAAA CATGAATTGG CCGCCATAGA TATGCTTGGA 
AAGTCACAGG CTGGTAGTGA TCTGGAGCGT AGCAGCACCA GCAGGCGTGA ACAATTACGC 
GGGTTCTTGC AGGAACTCCA AGAGCAAATC GATAATGCCG ACACTGAATC AGTGTGCACT 
ATGAGCTCTT GCACGTCGAT ATGCAGCAGC CCAGGAGATG CGGAATTCTT TGCGGAATTG 
TTCTCCCCAC CCCCTAGCAA CGCCCCGCAG GAGAGCATGG ATGACAGTAA TTTTTTAGAG 
ATGTATGTAG ATGGTTACAA CCCCTCCTGT GGCAGTTATG GAACCAGTAA CCCAAGTGGC 
AAAGAGGTCT CACAGGACAG TGCTAATATC GGCCAGTCCA ATTTACAGCG TGCTTCCGAC 
AGCCACAATA ACACTAACGT GGGGGACGTC TTCCATGATA TGCTGTCAAC CTCCACAATG 
CACATGCTGA ACGAGGATTA TTTCTCACCT TTTGATGATA TGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947223.2
CDS	683..5950
Translation	MSPCLPKESHTSVPGPSENGQDERSQQQYGMCKVSACNSASPHGDRAPSVEFALRDSNQPNHPQEALNAFHEEAVQNQVMGMTIASINGPSPLKWNVPATKDMHTLSQSVNAANDPPTCINQKTDAGIAEVLNGFTKAEANMCHSVSVLSTCAPLGSPRLRLSALKEGHKQSDIRSASCSSACLEKVASETALAAMSDSNMKKEDSFNMAFELLPSSPPLHSFFGASQNVHTSEESSSLLGTVGQTKENTISVKSIGSSNVVLNEGKGETSQFTILSKSHSEVEDVQKVCVRLPSSPLLETALQMKAYAHSDKLSAFNNNPCLEKVLPEATPVVLSETYREVQDCFGSLPSSDLHENEPPLEERSLVDKSNSSRNYTCLEKVVPEATLLLKEYFSESCSKVQDDEKCFGSLPSSALQRKNPLIEKNGPSDKSSASSNNVCLEEGEPEAVPLLEDCKSQTLVVPSTCSQLSITDKIANSLGVHNSSDSCQLISEIHHDHPSDGNDLTVSHIAFNVGGADKINKVKSLQVTPLPEVISTELCRGKSNSQSTCEPPSVPVLSCKLSSEQKDEGTLMELSSSLHSETTEEEECGNKMALGSSTLDGEISDYKKLNSQIKPLPQAIESLVMDTCDSNLCNNINLKDSSKIISEEATVPHCFQPEQHVVYSHPMSDPAVTCVTHGTGSEMKALDTPRKESNPVCKELRERNVNPMKELYISLNNAPTDLSNSLCSDTTNVEESRDATANASSLSNGEILHLYHKTSSPALVDPKAEANTVLGLCDSVLSKNSTMGSSDSINQEKAIATTFAHPGQSVLFSHGASTITVSPGLFHSAVQALDVSDTPELGCSPVCKEPTVVQDKSDGSFDTSLGHFEREKQNESFETDSSVMLTDDHIKDPKDVDVVLSLPEGSLEDCCPKKKSNCSENMHVALKGTSTNPSLELGSHDAPCEKKLLQWRNTLFLCWLDCILAALVHSASLNSIVAQRRVAESSIIHLLLEKYKEANALIKSTSRRGRPKNLSQAENILNKVRMTIFDEIKPLLKCELGDKESPVFALPLLLQLVPEIESCFVHSGVWEFTCKLCGYQYQNKHQRTVSTFTKILPEWTPLNAIHKAPCNKCLGTDQIRAFRIKKIHDLFILHFVEGLPSNDLGMYSFHFEGCLYEVKTVIQYSEDHFSSWIANEDGTWLESDDLKGYFCIKQNCFEVPASEIHIVIWEKKITNSTGAHHAMEPETKNKALNSLSTSPSSLSQTLHLSPSAAPNAASSPLRDAPNASSVLSGMEGFHDDDIITLTLIEIPIDASGNVIENNSEAAQQPSLIAETHSSPVTLKAVADGQCDQNLCSGTMKESSFAGGSLPVPNISSPPAPNISSPPAPNISSPPAPHISSPPAPHISSPPAPHISSPPAPHISSPPAPHISSPPAPHISSPPAPNSASPPAPNSASTPLPNSSSSKKAFVGSWMKSLLNKNPLFLTSNVPCANKKTVAKTPIRFPLLKTTDLQASTKQAQSFDGFKAKRVGNSFSSDNCLGVGPTKDNSTAPKPAISSQTFQPLVSNRLKNGRLGLGNTKDLAKNVSLSKEDKVLRLRLKMLKKLKAKKHELAAIDMLGKSQAGSDLERSSTSRREQLRGFLQELQEQIDNADTESVCTMSSCTSICSSPGDAEFFAELFSPPPSNAPQESMDDSNFLEMYVDGYNPSCGSYGTSNPSGKEVSQDSANIGQSNLQRASDSHNNTNVGDVFHDMLSTSTMHMLNEDYFSPFDDMF

Target ORF information:

RefSeq Version	XM_018091734.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin specific peptidase like 1 (uspl1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018091734.2

ORF Insert Sequence:

ATGTCTCCGT GTTTACCTAA AGAATCTCAT ACAAGTGTAC CTGGCCCTTC AGAGAATGGT 
CAGGATGAGA GAAGTCAGCA GCAATATGGA ATGTGCAAAG TTTCAGCTTG CAATTCTGCA 
AGTCCACATG GTGACAGGGC ACCTTCAGTT GAATTTGCTC TGCGGGACTC AAACCAACCT 
AATCACCCGC AGGAAGCCTT AAATGCATTT CACGAAGAAG CTGTGCAAAA TCAAGTTATG 
GGAATGACCA TTGCATCAAT TAATGGTCCT TCTCCTTTAA AATGGAATGT ACCTGCTACT 
AAAGATATGC ACACATTATC ACAGTCGGTG AATGCTGCTA ATGACCCACC AACATGTATA 
AACCAAAAGA CAGATGCTGG AATAGCTGAG GTTTTAAATG GATTTACAAA GGCTGAAGCC 
AATATGTGCC ATTCAGTATC TGTCCTTAGT ACTTGTGCAC CTTTAGGCTC ACCTCGTTTA 
AGGCTTAGTG CTCTAAAGGA GGGACATAAA CAGAGTGATA TACGGAGTGC CTCCTGCAGT 
AGTGCTTGTC TTGAAAAAGT AGCATCAGAA ACAGCTCTTG CCGCCATGTC TGACAGCAAC 
ATGAAGAAAG AGGATAGTTT CAACATGGCA TTTGAACTTT TACCGTCGTC TCCTCCATTG 
CATTCATTTT TTGGGGCTTC ACAAAATGTG CACACATCTG AAGAATCATC CTCTTTGCTT 
GGAACAGTGG GTCAAACAAA AGAAAATACC ATAAGTGTTA AATCAATTGG ATCCAGCAAT 
GTTGTTCTTA ATGAAGGAAA GGGGGAAACC TCCCAGTTTA CGATATTGTC CAAATCACAC 
AGTGAGGTAG AAGATGTTCA GAAAGTGTGT GTACGTTTGC CCTCTTCCCC ACTGCTTGAA 
ACAGCTTTAC AAATGAAAGC GTATGCCCAC AGTGACAAGC TCAGTGCCTT CAACAATAAT 
CCATGTCTTG AGAAAGTATT ACCAGAGGCA ACCCCAGTGG TTTTATCAGA AACATATAGG 
GAGGTACAAG ATTGTTTTGG ATCTTTACCT TCATCTGATT TGCATGAAAA TGAGCCTCCA 
CTGGAAGAGA GATCCCTGGT TGATAAATCT AATAGCTCCA GAAATTATAC TTGCCTTGAG 
AAAGTAGTAC CAGAGGCAAC CTTATTGTTA AAAGAGTACT TTTCAGAATC CTGTAGTAAG 
GTACAAGATG ATGAGAAATG TTTTGGATCT TTACCTTCAT CTGCTTTGCA AAGAAAAAAT 
CCTCTAATTG AAAAAAACGG ACCGAGTGAC AAATCCAGTG CATCCAGCAA TAATGTTTGC 
CTTGAGGAAG GAGAACCAGA GGCAGTCCCA TTGTTGGAAG ACTGTAAAAG TCAAACCCTT 
GTTGTACCTT CCACATGCAG TCAATTGTCC ATAACAGATA AAATAGCAAA CAGTCTTGGA 
GTACATAATT CCTCAGATTC ATGTCAGCTC ATCAGTGAAA TTCATCATGA TCATCCAAGC 
GATGGAAATG ATCTCACTGT GTCCCATATT GCCTTCAATG TTGGTGGTGC TGACAAAATC 
AATAAAGTTA AAAGCCTGCA GGTAACTCCA CTTCCAGAGG TTATATCTAC AGAACTATGT 
AGGGGCAAGA GCAACAGTCA AAGCACTTGT GAACCTCCAT CCGTGCCAGT TCTGTCATGT 
AAGCTTTCTT CTGAGCAAAA AGATGAAGGT ACATTGATGG AATTGTCTAG CTCTTTACAT 
AGTGAAACAA CTGAGGAGGA AGAATGTGGA AATAAAATGG CTCTTGGAAG TTCAACATTG 
GATGGGGAAA TATCTGACTA TAAGAAACTA AACAGTCAAA TTAAGCCTTT GCCACAAGCA 
ATAGAAAGCT TGGTCATGGA CACGTGTGAT AGCAATCTGT GCAATAATAT TAATTTAAAA 
GATAGCAGCA AAATAATTTC AGAGGAAGCT ACAGTACCAC ATTGTTTTCA GCCAGAACAG 
CATGTTGTAT ACAGCCATCC TATGTCTGAC CCTGCAGTTA CTTGTGTGAC TCATGGCACT 
GGATCTGAAA TGAAAGCATT GGATACACCA AGGAAGGAGT CTAATCCAGT GTGTAAGGAA 
CTCCGTGAAA GAAATGTAAA TCCAATGAAA GAATTGTATA TATCTCTGAA CAATGCACCA 
ACTGATTTGT CTAACTCTTT GTGCAGTGAC ACTACTAATG TGGAAGAATC TAGGGATGCC 
ACAGCAAATG CAAGTTCTTT ATCCAACGGA GAAATATTGC ATTTATATCA CAAAACAAGC 
AGCCCAGCTC TTGTTGATCC AAAAGCAGAA GCAAATACAG TTTTAGGCTT GTGTGACAGT 
GTCCTCAGCA AAAACTCTAC AATGGGAAGT AGTGATAGCA TTAATCAGGA GAAAGCTATA 
GCAACAACTT TTGCACACCC AGGACAGAGT GTGTTGTTCA GCCATGGGGC ATCTACAATT 
ACAGTCTCCC CTGGTTTATT TCATAGTGCT GTACAGGCAT TAGATGTTTC TGATACACCA 
GAGTTGGGGT GCAGTCCTGT GTGTAAGGAA CCCACTGTTG TACAAGATAA AAGCGATGGA 
TCCTTTGATA CTTCTTTGGG CCATTTTGAA AGAGAAAAGC AAAATGAGAG CTTTGAAACA 
GACAGTTCTG TAATGCTTAC TGATGATCAC ATAAAGGACC CAAAGGATGT AGATGTTGTA 
TTGTCTTTGC CAGAGGGGAG CTTGGAAGAT TGCTGTCCCA AAAAAAAATC AAACTGCTCA 
GAGAACATGC ATGTAGCCTT AAAGGGTACC TCCACAAACC CTTCCCTTGA ACTCGGTTCC 
CACGATGCCC CGTGTGAAAA GAAGCTGCTT CAATGGAGAA ACACCCTCTT CCTCTGCTGG 
TTGGACTGCA TATTGGCAGC ACTGGTGCAT TCTGCTAGCC TGAATAGCAT TGTGGCACAG 
CGAAGGGTTG CAGAAAGCTC AATCATTCAC CTTCTTCTGG AGAAATACAA GGAGGCCAAT 
GCATTAATAA AAAGCACATC CAGACGAGGA CGTCCAAAGA ATTTATCTCA AGCTGAAAAC 
ATCCTGAATA AAGTGCGAAT GACCATCTTT GATGAAATAA AGCCCTTGTT AAAGTGTGAA 
TTGGGAGATA AAGAGAGCCC TGTGTTTGCT TTGCCGTTAC TGCTGCAGCT GGTTCCAGAG 
ATAGAATCCT GTTTTGTGCA CTCAGGTGTA TGGGAGTTTA CATGCAAGCT ATGTGGCTAC 
CAATACCAAA ATAAGCACCA ACGGACTGTG TCCACATTCA CTAAGATCTT ACCTGAATGG 
ACACCCCTAA ATGCTATCCA TAAAGCTCCT TGCAACAAAT GCCTGGGTAC AGATCAGATA 
CGTGCTTTTC GTATTAAAAA GATCCATGAC CTATTCATAC TTCACTTTGT GGAAGGCCTG 
CCAAGCAATG ATTTAGGCAT GTACTCGTTT CACTTTGAAG GCTGCCTGTA TGAAGTAAAG 
ACTGTCATCC AGTATAGTGA AGACCATTTC TCATCTTGGA TTGCTAATGA GGATGGCACA 
TGGCTGGAAT CTGATGACCT GAAAGGGTAT TTCTGCATAA AACAGAACTG TTTTGAGGTT 
CCTGCTTCAG AAATTCATAT TGTAATATGG GAAAAGAAGA TTACCAACAG CACTGGAGCA 
CACCATGCAA TGGAACCTGA AACAAAAAAC AAAGCACTTA ATTCTTTATC TACTTCACCT 
TCATCTTTAT CTCAGACCCT GCACCTGTCT CCTTCAGCTG CTCCGAATGC TGCATCTTCA 
CCTCTAAGAG ATGCTCCAAA TGCTTCCAGC GTACTCTCTG GAATGGAGGG TTTTCATGAT 
GACGACATCA TAACGCTCAC TCTCATTGAA ATTCCAATTG ATGCATCGGG TAATGTGATT 
GAGAACAACT CTGAGGCAGC CCAGCAGCCA AGTCTCATTG CTGAAACACA TTCATCCCCT 
GTAACCCTCA AAGCAGTTGC TGATGGGCAA TGTGATCAAA ATCTATGTTC TGGTACAATG 
AAGGAAAGCT CTTTCGCTGG TGGTTCTCTT CCAGTTCCTA ACATTTCCAG CCCTCCTGCC 
CCTAACATTT CCAGCCCTCC TGCCCCTAAC ATTTCCAGCC CTCCTGCCCC TCACATTTCC 
AGCCCTCCTG CCCCTCACAT TTCCAGCCCT CCTGCCCCTC ACATTTCCAG CCCTCCTGCC 
CCTCACATTT CCAGCCCTCC TGCCCCTCAC ATTTCCAGCC CTCCTGCCCC TCACATTTCC 
AGCCCTCCTG CCCCTAACAG CGCCAGTCCC CCTGCCCCTA ACAGCGCCAG TACTCCCCTC 
CCTAACAGTT CCAGTTCAAA AAAAGCCTTT GTTGGAAGTT GGATGAAGAG TCTACTAAAT 
AAAAACCCTT TGTTCCTGAC ATCTAATGTG CCCTGTGCAA ATAAGAAAAC TGTGGCAAAA 
ACTCCAATCC GATTTCCCCT GCTGAAGACA ACAGATTTGC AGGCATCTAC TAAACAGGCC 
CAGAGCTTTG ATGGTTTTAA AGCTAAACGT GTCGGTAATT CATTCAGCTC GGACAATTGC 
CTTGGTGTAG GTCCCACGAA GGATAATTCC ACTGCACCCA AACCTGCCAT CTCTAGCCAA 
ACGTTCCAGC CACTGGTTTC AAATCGACTT AAAAATGGGC GGTTAGGACT CGGTAATACC 
AAAGACTTGG CAAAAAATGT TTCTCTGTCT AAGGAGGATA AGGTGCTTAG GCTTAGACTG 
AAAATGCTGA AAAAGTTGAA GGCTAAGAAA CATGAATTGG CCGCCATAGA TATGCTTGGA 
AAGTCACAGG CTGGTAGTGA TCTGGAGCGT AGCAGCACCA GCAGGCGTGA ACAATTACGC 
GGGTTCTTGC AGGAACTCCA AGAGCAAATC GATAATGCCG ACACTGAATC AGTGTGCACT 
ATGAGCTCTT GCACGTCGAT ATGCAGCAGC CCAGGAGATG CGGAATTCTT TGCGGAATTG 
TTCTCCCCAC CCCCTAGCAA CGCCCCGCAG GAGAGCATGG ATGACAGTAA TTTTTTAGAG 
ATGTATGTAG ATGGTTACAA CCCCTCCTGT GGCAGTTATG GAACCAGTAA CCCAAGTGGC 
AAAGAGGTCT CACAGGACAG TGCTAATATC GGCCAGTCCA ATTTACAGCG TGCTTCCGAC 
AGCCACAATA ACACTAACGT GGGGGACGTC TTCCATGATA TGCTGTCAAC CTCCACAATG 
CACATGCTGA ACGAGGATTA TTTCTCACCT TTTGATGATA TGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

uspl1 ( XM_018091733.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947222.1 Xenopus tropicalis ubiquitin specific peptidase like 1 (uspl1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa42866
Clone ID Related Accession (Same CDS sequence)	XM_018091733.1 , XM_018091731.1 , XM_018091732.1
Accession Version	XM_018091733.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5790bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	SUMO-specific isopeptidase USPL1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTAAAG TAAGCCAATG GTTCACAACG ATGGCTTCAG AGACTCCTGG ATCTACAGTG 
TCACTGGTTG GACAAGGGTC TATTTTAGAT AAATCTTCAC TCCACACGGT GGGGTGCTTG 
GGAAAAAATG TTGACCCCTG TGCAGCTCCT GCTGATGGAT TTTGCCCAGT ATGCAAGGCC 
AAAGGCCAGC TGCAAGCTCT CCGGACATAT CGCTTTAATT TCACTCAGTC TATATATCTC 
TGTGCCAATC CACAGTGCAT ATATCCCTTG GGATATACAC CTCTGGATAA CATAATTGCA 
AACACAGCAG ATCTACAGAA ACACCATAGC CCTGGAAAGC AGAAGAAGCG GTGTCTTTTA 
GACACCTCTG TAACAACTAA CTGTGTGAAG AAGCATAAGA CTGATTCTTT TGTCTCTGGA 
GAGTACATTT CAGAAAACCT ATTTTTATCT GCACAAAATA AAGGCTACAA ATCAATGTCT 
CCGTGTTTAC CTAAAGAATC TCATACAAGT GTACCTGGCC CTTCAGAGAA TGGTCAGGAT 
GAGAGAAGTC AGCAGCAATA TGGAATGTGC AAAGTTTCAG CTTGCAATTC TGCAAGTCCA 
CATGGTGACA GGGCACCTTC AGTTGAATTT GCTCTGCGGG ACTCAAACCA ACCTAATCAC 
CCGCAGGAAG CCTTAAATGC ATTTCACGAA GAAGCTGTGC AAAATCAAGT TATGGGAATG 
ACCATTGCAT CAATTAATGG TCCTTCTCCT TTAAAATGGA ATGTACCTGC TACTAAAGAT 
ATGCACACAT TATCACAGTC GGTGAATGCT GCTAATGACC CACCAACATG TATAAACCAA 
AAGACAGATG CTGGAATAGC TGAGGTTTTA AATGGATTTA CAAAGGCTGA AGCCAATATG 
TGCCATTCAG TATCTGTCCT TAGTACTTGT GCACCTTTAG GCTCACCTCG TTTAAGGCTT 
AGTGCTCTAA AGGAGGGACA TAAACAGAGT GATATACGGA GTGCCTCCTG CAGTAGTGCT 
TGTCTTGAAA AAGTAGCATC AGAAACAGCT CTTGCCGCCA TGTCTGACAG CAACATGAAG 
AAAGAGGATA GTTTCAACAT GGCATTTGAA CTTTTACCGT CGTCTCCTCC ATTGCATTCA 
TTTTTTGGGG CTTCACAAAA TGTGCACACA TCTGAAGAAT CATCCTCTTT GCTTGGAACA 
GTGGGTCAAA CAAAAGAAAA TACCATAAGT GTTAAATCAA TTGGATCCAG CAATGTTGTT 
CTTAATGAAG GAAAGGGGGA AACCTCCCAG TTTACGATAT TGTCCAAATC ACACAGTGAG 
GTAGAAGATG TTCAGAAAGT GTGTGTACGT TTGCCCTCTT CCCCACTGCT TGAAACAGCT 
TTACAAATGA AAGCGTATGC CCACAGTGAC AAGCTCAGTG CCTTCAACAA TAATCCATGT 
CTTGAGAAAG TATTACCAGA GGCAACCCCA GTGGTTTTAT CAGAAACATA TAGGGAGGTA 
CAAGATTGTT TTGGATCTTT ACCTTCATCT GATTTGCATG AAAATGAGCC TCCACTGGAA 
GAGAGATCCC TGGTTGATAA ATCTAATAGC TCCAGAAATT ATACTTGCCT TGAGAAAGTA 
GTACCAGAGG CAACCTTATT GTTAAAAGAG TACTTTTCAG AATCCTGTAG TAAGGTACAA 
GATGATGAGA AATGTTTTGG ATCTTTACCT TCATCTGCTT TGCAAAGAAA AAATCCTCTA 
ATTGAAAAAA ACGGACCGAG TGACAAATCC AGTGCATCCA GCAATAATGT TTGCCTTGAG 
GAAGGAGAAC CAGAGGCAGT CCCATTGTTG GAAGACTGTA AAAGTCAAAC CCTTGTTGTA 
CCTTCCACAT GCAGTCAATT GTCCATAACA GATAAAATAG CAAACAGTCT TGGAGTACAT 
AATTCCTCAG ATTCATGTCA GCTCATCAGT GAAATTCATC ATGATCATCC AAGCGATGGA 
AATGATCTCA CTGTGTCCCA TATTGCCTTC AATGTTGGTG GTGCTGACAA AATCAATAAA 
GTTAAAAGCC TGCAGGTAAC TCCACTTCCA GAGGTTATAT CTACAGAACT ATGTAGGGGC 
AAGAGCAACA GTCAAAGCAC TTGTGAACCT CCATCCGTGC CAGTTCTGTC ATGTAAGCTT 
TCTTCTGAGC AAAAAGATGA AGGTACATTG ATGGAATTGT CTAGCTCTTT ACATAGTGAA 
ACAACTGAGG AGGAAGAATG TGGAAATAAA ATGGCTCTTG GAAGTTCAAC ATTGGATGGG 
GAAATATCTG ACTATAAGAA ACTAAACAGT CAAATTAAGC CTTTGCCACA AGCAATAGAA 
AGCTTGGTCA TGGACACGTG TGATAGCAAT CTGTGCAATA ATATTAATTT AAAAGATAGC 
AGCAAAATAA TTTCAGAGGA AGCTACAGTA CCACATTGTT TTCAGCCAGA ACAGCATGTT 
GTATACAGCC ATCCTATGTC TGACCCTGCA GTTACTTGTG TGACTCATGG CACTGGATCT 
GAAATGAAAG CATTGGATAC ACCAAGGAAG GAGTCTAATC CAGTGTGTAA GGAACTCCGT 
GAAAGAAATG TAAATCCAAT GAAAGAATTG TATATATCTC TGAACAATGC ACCAACTGAT 
TTGTCTAACT CTTTGTGCAG TGACACTACT AATGTGGAAG AATCTAGGGA TGCCACAGCA 
AATGCAAGTT CTTTATCCAA CGGAGAAATA TTGCATTTAT ATCACAAAAC AAGCAGCCCA 
GCTCTTGTTG ATCCAAAAGC AGAAGCAAAT ACAGTTTTAG GCTTGTGTGA CAGTGTCCTC 
AGCAAAAACT CTACAATGGG AAGTAGTGAT AGCATTAATC AGGAGAAAGC TATAGCAACA 
ACTTTTGCAC ACCCAGGACA GAGTGTGTTG TTCAGCCATG GGGCATCTAC AATTACAGTC 
TCCCCTGGTT TATTTCATAG TGCTGTACAG GCATTAGATG TTTCTGATAC ACCAGAGTTG 
GGGTGCAGTC CTGTGTGTAA GGAACCCACT GTTGTACAAG ATAAAAGCGA TGGATCCTTT 
GATACTTCTT TGGGCCATTT TGAAAGAGAA AAGCAAAATG AGAGCTTTGA AACAGACAGT 
TCTGTAATGC TTACTGATGA TCACATAAAG GATCCAAAGG ATGTAGATGT TGTATTGTCT 
TTGCCAGAGG GGAGCTTGGA AGATTGCTGT CCCAAAAAAA AATCAAACTG CTCAGAGAAC 
ATGCATGTAG CCTTAAAGGG TACCTCCACA AACCCTTCCC TTGAACTCGG TTCCCACGAT 
GCCCCGTGTG AAAAGAAGCT GCTTCAATGG AGAAACACCC TCTTCCTCTG CTGGTTGGAC 
TGCATATTGG CAGCACTGGT GCATTCTGCT AGCCTGAATA GCATTGTGGC ACAGCGAAGG 
GTTGCAGAAA GCTCAATCAT TCACCTTCTT CTGGAGAAAT ACAAGGAGGC CAATGCATTA 
ATAAAAAGCA CATCCAGACG AGGACGTCCA AAGAATTTAT CTCAAGCTGA AAACATCCTG 
AATAAAGTGC GAATGACCAT CTTTGATGAA ATAAAGCCCT TGTTAAAGTG TGAATTGGGA 
GATAAAGAGA GCCCTGTGTT TGCTTTGCCG TTACTGCTGC AGCTGGTTCC AGAGATAGAA 
TCCTGTTTTG TGCACTCAGG TGTATGGGAG TTTACATGCA AGCTATGTGG CTACCAATAC 
CAAAATAAGC ACCAACGGAC TGTGTCCACA TTCACTAAGA TCTTACCTGA ATGGACACCC 
CTAAATGCTA TCCATAAAGC TCCTTGCAAC AAATGCCTGG GTACAGATCA GATACGTGCT 
TTTCGTATTA AAAAGATCCA TGACCTATTC ATACTTCACT TTGTGGAAGG CCTGCCAAGC 
AATGATTTAG GCATGTACTC GTTTCACTTT GAAGGCTGCC TGTATGAAGT AAAGACTGTC 
ATCCAGTATA GTGAAGACCA TTTCTCATCT TGGATTGCTA ATGAGGATGG CACATGGCTG 
GAATCTGATG ACCTGAAAGG GTATTTCTGC ATAAAACAGA ACTGTTTTGA GGTTCCTGCT 
TCAGAAATTC ATATTGTAAT ATGGGAAAAG AAGATTACCA ACAGCACTGG AGCACACCAT 
GCAATGGAAC CTGAAACAAA AAACAAAGCA CTTAATTCTT TATCTACTTC ACCTTCATCT 
TTATCTCAGA CCCTGCACCT GTCTCCTTCA GCTGCTCCGA ATGCTGCATC TTCACCTCTA 
AGAGATGCCC CAAATGCTTC CAGCGTACTC TCTGGAATGG AGGGTTTTCA TGATGACGAC 
ATCATAACGC TCACTCTCAT TGAAATTCCA ATTGATGCAT CGGGTAATGT GATTGAGAAC 
AACTCTGAGG CAGCCCAGCA GCCAAGTCTC ATTGCTGAAA CACATTCATC CCCTGTAACC 
CTCAAAGCAG TTGCTGATGG GCAATGTGAT CAAAATCTAT GTTCTGGTAC AATGAAGGAA 
AGCTCTTTCG CTGGTGGTTC TCTTCCAGTT CCTAACATTT CCAGCCCTCC TGCCCCTAAC 
ATTTCCAGCC CTCCTGCCCC TAACATTTCC AGCCCTCCTG CCCCTAACAT TTCCAGCCCT 
CCTGCCTCTA ACATTTCCAG CCCTCCTGCC CCTCACATTT CCAGCCCTCC TGCCCCTCAC 
ATTTCCAGCC CTCCTGCCCC TCACATTTCC AGCCCTCCTG CCCCTCACAT TTCCAGCCCT 
CCTGCCCCTC ACATTTCCAG CCCTCCTGCC CCTCACATTT CCAGCCCTCC TGCCCCTAAC 
AGCGCCAGTC CCCCTGCCCC TAACAGCGCC AGTACTCCCC TCCCTAACAG TTCCAGTTCA 
AAAAAAGCCT TTGTTGGAAG TTGGATGAAG AGTCTACTAA ATAAAAACCC TTTGTTCCTG 
ACATCTAATG TGCCCTGTGC AAATAAGAAA ACTGTGGCAA AAACTCCAAT CCGATTTCCC 
CTGCTGAAGA CAACAGATTT GCAGGCATCT ACTAAACAGG CCCAGAGCTT TGATGGTTTT 
AAAGCTAAAC GTGTCGGTAA TTCATTCAGC TCGGACAATT GCCTTGGTGT AGGTCCCACG 
AAGGATAATT CCACTGCACC CAAACCTGCC ATCTCTAGCC AAACGTTCCA GCCACTGGTT 
TCAAATCGAC TTAAAAATGG GCGGTTAGGA CTCGGTAATA CCAAAGACTT GGCAAAAAAT 
GTTTCTCTGT CTAAGGAGGA TAAGGTGCTT AGGCTTAGAC TGAAAATGCT GAAAAAGTTG 
AAGGCTAAGA AACATGAATT GGCCGCCATA GATATGCTTG GAAAGTCACA GGCTGGTAGT 
GATCTGGAGC GTAGCAGCAC CAGCAGGCGT GAACAATTAC GCGGGTTCTT GCAGGAACTC 
CAAGAGCAAA TCGATAATGC CGACACTGAA TCAGTGTGCA CTATGAGCTC TTGCACGTCG 
ATATGCAGCA GCCCAGGAGA TGCGGAATTC TTTGCGGAAT TGTTCTCCCC ACCCCCTAGC 
AACGCCCCGC AGGAGAGCAT GGATGACAGT AATTTTTTAG AGATGTATGT AGATGGTTAC 
AACCCCTCCT GTGGCAGTTA TGGAACCAGT AACCCAAGTG GCAAAGAGGT CTCACAGGAC 
AGTGCTAATA TCGGCCAGTC CAATTTACAG CGTGCTTCCG ACAGCCACAA TAACACTAAC 
GTGGGGGACG TCTTCCATGA TATGCTGTCA ACCTCCACAA TGCACATGCT GAACGAGGAT 
TATTTCTCAC CTTTTGATGA TATGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947222.1
CDS	528..6317
Translation	MTKVSQWFTTMASETPGSTVSLVGQGSILDKSSLHTVGCLGKNVDPCAAPADGFCPVCKAKGQLQALRTYRFNFTQSIYLCANPQCIYPLGYTPLDNIIANTADLQKHHSPGKQKKRCLLDTSVTTNCVKKHKTDSFVSGEYISENLFLSAQNKGYKSMSPCLPKESHTSVPGPSENGQDERSQQQYGMCKVSACNSASPHGDRAPSVEFALRDSNQPNHPQEALNAFHEEAVQNQVMGMTIASINGPSPLKWNVPATKDMHTLSQSVNAANDPPTCINQKTDAGIAEVLNGFTKAEANMCHSVSVLSTCAPLGSPRLRLSALKEGHKQSDIRSASCSSACLEKVASETALAAMSDSNMKKEDSFNMAFELLPSSPPLHSFFGASQNVHTSEESSSLLGTVGQTKENTISVKSIGSSNVVLNEGKGETSQFTILSKSHSEVEDVQKVCVRLPSSPLLETALQMKAYAHSDKLSAFNNNPCLEKVLPEATPVVLSETYREVQDCFGSLPSSDLHENEPPLEERSLVDKSNSSRNYTCLEKVVPEATLLLKEYFSESCSKVQDDEKCFGSLPSSALQRKNPLIEKNGPSDKSSASSNNVCLEEGEPEAVPLLEDCKSQTLVVPSTCSQLSITDKIANSLGVHNSSDSCQLISEIHHDHPSDGNDLTVSHIAFNVGGADKINKVKSLQVTPLPEVISTELCRGKSNSQSTCEPPSVPVLSCKLSSEQKDEGTLMELSSSLHSETTEEEECGNKMALGSSTLDGEISDYKKLNSQIKPLPQAIESLVMDTCDSNLCNNINLKDSSKIISEEATVPHCFQPEQHVVYSHPMSDPAVTCVTHGTGSEMKALDTPRKESNPVCKELRERNVNPMKELYISLNNAPTDLSNSLCSDTTNVEESRDATANASSLSNGEILHLYHKTSSPALVDPKAEANTVLGLCDSVLSKNSTMGSSDSINQEKAIATTFAHPGQSVLFSHGASTITVSPGLFHSAVQALDVSDTPELGCSPVCKEPTVVQDKSDGSFDTSLGHFEREKQNESFETDSSVMLTDDHIKDPKDVDVVLSLPEGSLEDCCPKKKSNCSENMHVALKGTSTNPSLELGSHDAPCEKKLLQWRNTLFLCWLDCILAALVHSASLNSIVAQRRVAESSIIHLLLEKYKEANALIKSTSRRGRPKNLSQAENILNKVRMTIFDEIKPLLKCELGDKESPVFALPLLLQLVPEIESCFVHSGVWEFTCKLCGYQYQNKHQRTVSTFTKILPEWTPLNAIHKAPCNKCLGTDQIRAFRIKKIHDLFILHFVEGLPSNDLGMYSFHFEGCLYEVKTVIQYSEDHFSSWIANEDGTWLESDDLKGYFCIKQNCFEVPASEIHIVIWEKKITNSTGAHHAMEPETKNKALNSLSTSPSSLSQTLHLSPSAAPNAASSPLRDAPNASSVLSGMEGFHDDDIITLTLIEIPIDASGNVIENNSEAAQQPSLIAETHSSPVTLKAVADGQCDQNLCSGTMKESSFAGGSLPVPNISSPPAPNISSPPAPNISSPPAPNISSPPASNISSPPAPHISSPPAPHISSPPAPHISSPPAPHISSPPAPHISSPPAPHISSPPAPNSASPPAPNSASTPLPNSSSSKKAFVGSWMKSLLNKNPLFLTSNVPCANKKTVAKTPIRFPLLKTTDLQASTKQAQSFDGFKAKRVGNSFSSDNCLGVGPTKDNSTAPKPAISSQTFQPLVSNRLKNGRLGLGNTKDLAKNVSLSKEDKVLRLRLKMLKKLKAKKHELAAIDMLGKSQAGSDLERSSTSRREQLRGFLQELQEQIDNADTESVCTMSSCTSICSSPGDAEFFAELFSPPPSNAPQESMDDSNFLEMYVDGYNPSCGSYGTSNPSGKEVSQDSANIGQSNLQRASDSHNNTNVGDVFHDMLSTSTMHMLNEDYFSPFDDMF

Target ORF information:

RefSeq Version	XM_018091733.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin specific peptidase like 1 (uspl1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018091733.1

ORF Insert Sequence:

ATGACTAAAG TAAGCCAATG GTTCACAACG ATGGCTTCAG AGACTCCTGG ATCTACAGTG 
TCACTGGTTG GACAAGGGTC TATTTTAGAT AAATCTTCAC TCCACACGGT GGGGTGCTTG 
GGAAAAAATG TTGACCCCTG TGCAGCTCCT GCTGATGGAT TTTGCCCAGT ATGCAAGGCC 
AAAGGCCAGC TGCAAGCTCT CCGGACATAT CGCTTTAATT TCACTCAGTC TATATATCTC 
TGTGCCAATC CACAGTGCAT ATATCCCTTG GGATATACAC CTCTGGATAA CATAATTGCA 
AACACAGCAG ATCTACAGAA ACACCATAGC CCTGGAAAGC AGAAGAAGCG GTGTCTTTTA 
GACACCTCTG TAACAACTAA CTGTGTGAAG AAGCATAAGA CTGATTCTTT TGTCTCTGGA 
GAGTACATTT CAGAAAACCT ATTTTTATCT GCACAAAATA AAGGCTACAA ATCAATGTCT 
CCGTGTTTAC CTAAAGAATC TCATACAAGT GTACCTGGCC CTTCAGAGAA TGGTCAGGAT 
GAGAGAAGTC AGCAGCAATA TGGAATGTGC AAAGTTTCAG CTTGCAATTC TGCAAGTCCA 
CATGGTGACA GGGCACCTTC AGTTGAATTT GCTCTGCGGG ACTCAAACCA ACCTAATCAC 
CCGCAGGAAG CCTTAAATGC ATTTCACGAA GAAGCTGTGC AAAATCAAGT TATGGGAATG 
ACCATTGCAT CAATTAATGG TCCTTCTCCT TTAAAATGGA ATGTACCTGC TACTAAAGAT 
ATGCACACAT TATCACAGTC GGTGAATGCT GCTAATGACC CACCAACATG TATAAACCAA 
AAGACAGATG CTGGAATAGC TGAGGTTTTA AATGGATTTA CAAAGGCTGA AGCCAATATG 
TGCCATTCAG TATCTGTCCT TAGTACTTGT GCACCTTTAG GCTCACCTCG TTTAAGGCTT 
AGTGCTCTAA AGGAGGGACA TAAACAGAGT GATATACGGA GTGCCTCCTG CAGTAGTGCT 
TGTCTTGAAA AAGTAGCATC AGAAACAGCT CTTGCCGCCA TGTCTGACAG CAACATGAAG 
AAAGAGGATA GTTTCAACAT GGCATTTGAA CTTTTACCGT CGTCTCCTCC ATTGCATTCA 
TTTTTTGGGG CTTCACAAAA TGTGCACACA TCTGAAGAAT CATCCTCTTT GCTTGGAACA 
GTGGGTCAAA CAAAAGAAAA TACCATAAGT GTTAAATCAA TTGGATCCAG CAATGTTGTT 
CTTAATGAAG GAAAGGGGGA AACCTCCCAG TTTACGATAT TGTCCAAATC ACACAGTGAG 
GTAGAAGATG TTCAGAAAGT GTGTGTACGT TTGCCCTCTT CCCCACTGCT TGAAACAGCT 
TTACAAATGA AAGCGTATGC CCACAGTGAC AAGCTCAGTG CCTTCAACAA TAATCCATGT 
CTTGAGAAAG TATTACCAGA GGCAACCCCA GTGGTTTTAT CAGAAACATA TAGGGAGGTA 
CAAGATTGTT TTGGATCTTT ACCTTCATCT GATTTGCATG AAAATGAGCC TCCACTGGAA 
GAGAGATCCC TGGTTGATAA ATCTAATAGC TCCAGAAATT ATACTTGCCT TGAGAAAGTA 
GTACCAGAGG CAACCTTATT GTTAAAAGAG TACTTTTCAG AATCCTGTAG TAAGGTACAA 
GATGATGAGA AATGTTTTGG ATCTTTACCT TCATCTGCTT TGCAAAGAAA AAATCCTCTA 
ATTGAAAAAA ACGGACCGAG TGACAAATCC AGTGCATCCA GCAATAATGT TTGCCTTGAG 
GAAGGAGAAC CAGAGGCAGT CCCATTGTTG GAAGACTGTA AAAGTCAAAC CCTTGTTGTA 
CCTTCCACAT GCAGTCAATT GTCCATAACA GATAAAATAG CAAACAGTCT TGGAGTACAT 
AATTCCTCAG ATTCATGTCA GCTCATCAGT GAAATTCATC ATGATCATCC AAGCGATGGA 
AATGATCTCA CTGTGTCCCA TATTGCCTTC AATGTTGGTG GTGCTGACAA AATCAATAAA 
GTTAAAAGCC TGCAGGTAAC TCCACTTCCA GAGGTTATAT CTACAGAACT ATGTAGGGGC 
AAGAGCAACA GTCAAAGCAC TTGTGAACCT CCATCCGTGC CAGTTCTGTC ATGTAAGCTT 
TCTTCTGAGC AAAAAGATGA AGGTACATTG ATGGAATTGT CTAGCTCTTT ACATAGTGAA 
ACAACTGAGG AGGAAGAATG TGGAAATAAA ATGGCTCTTG GAAGTTCAAC ATTGGATGGG 
GAAATATCTG ACTATAAGAA ACTAAACAGT CAAATTAAGC CTTTGCCACA AGCAATAGAA 
AGCTTGGTCA TGGACACGTG TGATAGCAAT CTGTGCAATA ATATTAATTT AAAAGATAGC 
AGCAAAATAA TTTCAGAGGA AGCTACAGTA CCACATTGTT TTCAGCCAGA ACAGCATGTT 
GTATACAGCC ATCCTATGTC TGACCCTGCA GTTACTTGTG TGACTCATGG CACTGGATCT 
GAAATGAAAG CATTGGATAC ACCAAGGAAG GAGTCTAATC CAGTGTGTAA GGAACTCCGT 
GAAAGAAATG TAAATCCAAT GAAAGAATTG TATATATCTC TGAACAATGC ACCAACTGAT 
TTGTCTAACT CTTTGTGCAG TGACACTACT AATGTGGAAG AATCTAGGGA TGCCACAGCA 
AATGCAAGTT CTTTATCCAA CGGAGAAATA TTGCATTTAT ATCACAAAAC AAGCAGCCCA 
GCTCTTGTTG ATCCAAAAGC AGAAGCAAAT ACAGTTTTAG GCTTGTGTGA CAGTGTCCTC 
AGCAAAAACT CTACAATGGG AAGTAGTGAT AGCATTAATC AGGAGAAAGC TATAGCAACA 
ACTTTTGCAC ACCCAGGACA GAGTGTGTTG TTCAGCCATG GGGCATCTAC AATTACAGTC 
TCCCCTGGTT TATTTCATAG TGCTGTACAG GCATTAGATG TTTCTGATAC ACCAGAGTTG 
GGGTGCAGTC CTGTGTGTAA GGAACCCACT GTTGTACAAG ATAAAAGCGA TGGATCCTTT 
GATACTTCTT TGGGCCATTT TGAAAGAGAA AAGCAAAATG AGAGCTTTGA AACAGACAGT 
TCTGTAATGC TTACTGATGA TCACATAAAG GATCCAAAGG ATGTAGATGT TGTATTGTCT 
TTGCCAGAGG GGAGCTTGGA AGATTGCTGT CCCAAAAAAA AATCAAACTG CTCAGAGAAC 
ATGCATGTAG CCTTAAAGGG TACCTCCACA AACCCTTCCC TTGAACTCGG TTCCCACGAT 
GCCCCGTGTG AAAAGAAGCT GCTTCAATGG AGAAACACCC TCTTCCTCTG CTGGTTGGAC 
TGCATATTGG CAGCACTGGT GCATTCTGCT AGCCTGAATA GCATTGTGGC ACAGCGAAGG 
GTTGCAGAAA GCTCAATCAT TCACCTTCTT CTGGAGAAAT ACAAGGAGGC CAATGCATTA 
ATAAAAAGCA CATCCAGACG AGGACGTCCA AAGAATTTAT CTCAAGCTGA AAACATCCTG 
AATAAAGTGC GAATGACCAT CTTTGATGAA ATAAAGCCCT TGTTAAAGTG TGAATTGGGA 
GATAAAGAGA GCCCTGTGTT TGCTTTGCCG TTACTGCTGC AGCTGGTTCC AGAGATAGAA 
TCCTGTTTTG TGCACTCAGG TGTATGGGAG TTTACATGCA AGCTATGTGG CTACCAATAC 
CAAAATAAGC ACCAACGGAC TGTGTCCACA TTCACTAAGA TCTTACCTGA ATGGACACCC 
CTAAATGCTA TCCATAAAGC TCCTTGCAAC AAATGCCTGG GTACAGATCA GATACGTGCT 
TTTCGTATTA AAAAGATCCA TGACCTATTC ATACTTCACT TTGTGGAAGG CCTGCCAAGC 
AATGATTTAG GCATGTACTC GTTTCACTTT GAAGGCTGCC TGTATGAAGT AAAGACTGTC 
ATCCAGTATA GTGAAGACCA TTTCTCATCT TGGATTGCTA ATGAGGATGG CACATGGCTG 
GAATCTGATG ACCTGAAAGG GTATTTCTGC ATAAAACAGA ACTGTTTTGA GGTTCCTGCT 
TCAGAAATTC ATATTGTAAT ATGGGAAAAG AAGATTACCA ACAGCACTGG AGCACACCAT 
GCAATGGAAC CTGAAACAAA AAACAAAGCA CTTAATTCTT TATCTACTTC ACCTTCATCT 
TTATCTCAGA CCCTGCACCT GTCTCCTTCA GCTGCTCCGA ATGCTGCATC TTCACCTCTA 
AGAGATGCCC CAAATGCTTC CAGCGTACTC TCTGGAATGG AGGGTTTTCA TGATGACGAC 
ATCATAACGC TCACTCTCAT TGAAATTCCA ATTGATGCAT CGGGTAATGT GATTGAGAAC 
AACTCTGAGG CAGCCCAGCA GCCAAGTCTC ATTGCTGAAA CACATTCATC CCCTGTAACC 
CTCAAAGCAG TTGCTGATGG GCAATGTGAT CAAAATCTAT GTTCTGGTAC AATGAAGGAA 
AGCTCTTTCG CTGGTGGTTC TCTTCCAGTT CCTAACATTT CCAGCCCTCC TGCCCCTAAC 
ATTTCCAGCC CTCCTGCCCC TAACATTTCC AGCCCTCCTG CCCCTAACAT TTCCAGCCCT 
CCTGCCTCTA ACATTTCCAG CCCTCCTGCC CCTCACATTT CCAGCCCTCC TGCCCCTCAC 
ATTTCCAGCC CTCCTGCCCC TCACATTTCC AGCCCTCCTG CCCCTCACAT TTCCAGCCCT 
CCTGCCCCTC ACATTTCCAG CCCTCCTGCC CCTCACATTT CCAGCCCTCC TGCCCCTAAC 
AGCGCCAGTC CCCCTGCCCC TAACAGCGCC AGTACTCCCC TCCCTAACAG TTCCAGTTCA 
AAAAAAGCCT TTGTTGGAAG TTGGATGAAG AGTCTACTAA ATAAAAACCC TTTGTTCCTG 
ACATCTAATG TGCCCTGTGC AAATAAGAAA ACTGTGGCAA AAACTCCAAT CCGATTTCCC 
CTGCTGAAGA CAACAGATTT GCAGGCATCT ACTAAACAGG CCCAGAGCTT TGATGGTTTT 
AAAGCTAAAC GTGTCGGTAA TTCATTCAGC TCGGACAATT GCCTTGGTGT AGGTCCCACG 
AAGGATAATT CCACTGCACC CAAACCTGCC ATCTCTAGCC AAACGTTCCA GCCACTGGTT 
TCAAATCGAC TTAAAAATGG GCGGTTAGGA CTCGGTAATA CCAAAGACTT GGCAAAAAAT 
GTTTCTCTGT CTAAGGAGGA TAAGGTGCTT AGGCTTAGAC TGAAAATGCT GAAAAAGTTG 
AAGGCTAAGA AACATGAATT GGCCGCCATA GATATGCTTG GAAAGTCACA GGCTGGTAGT 
GATCTGGAGC GTAGCAGCAC CAGCAGGCGT GAACAATTAC GCGGGTTCTT GCAGGAACTC 
CAAGAGCAAA TCGATAATGC CGACACTGAA TCAGTGTGCA CTATGAGCTC TTGCACGTCG 
ATATGCAGCA GCCCAGGAGA TGCGGAATTC TTTGCGGAAT TGTTCTCCCC ACCCCCTAGC 
AACGCCCCGC AGGAGAGCAT GGATGACAGT AATTTTTTAG AGATGTATGT AGATGGTTAC 
AACCCCTCCT GTGGCAGTTA TGGAACCAGT AACCCAAGTG GCAAAGAGGT CTCACAGGAC 
AGTGCTAATA TCGGCCAGTC CAATTTACAG CGTGCTTCCG ACAGCCACAA TAACACTAAC 
GTGGGGGACG TCTTCCATGA TATGCTGTCA ACCTCCACAA TGCACATGCT GAACGAGGAT 
TATTTCTCAC CTTTTGATGA TATGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

uspl1 ( XM_018091731.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947220.1 Xenopus tropicalis ubiquitin specific peptidase like 1 (uspl1), transcript variant X1, mRNA.

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CloneID	OXa42866
Clone ID Related Accession (Same CDS sequence)	XM_018091733.1 , XM_018091731.1 , XM_018091732.1
Accession Version	XM_018091731.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5790bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	SUMO-specific isopeptidase USPL1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTAAAG TAAGCCAATG GTTCACAACG ATGGCTTCAG AGACTCCTGG ATCTACAGTG 
TCACTGGTTG GACAAGGGTC TATTTTAGAT AAATCTTCAC TCCACACGGT GGGGTGCTTG 
GGAAAAAATG TTGACCCCTG TGCAGCTCCT GCTGATGGAT TTTGCCCAGT ATGCAAGGCC 
AAAGGCCAGC TGCAAGCTCT CCGGACATAT CGCTTTAATT TCACTCAGTC TATATATCTC 
TGTGCCAATC CACAGTGCAT ATATCCCTTG GGATATACAC CTCTGGATAA CATAATTGCA 
AACACAGCAG ATCTACAGAA ACACCATAGC CCTGGAAAGC AGAAGAAGCG GTGTCTTTTA 
GACACCTCTG TAACAACTAA CTGTGTGAAG AAGCATAAGA CTGATTCTTT TGTCTCTGGA 
GAGTACATTT CAGAAAACCT ATTTTTATCT GCACAAAATA AAGGCTACAA ATCAATGTCT 
CCGTGTTTAC CTAAAGAATC TCATACAAGT GTACCTGGCC CTTCAGAGAA TGGTCAGGAT 
GAGAGAAGTC AGCAGCAATA TGGAATGTGC AAAGTTTCAG CTTGCAATTC TGCAAGTCCA 
CATGGTGACA GGGCACCTTC AGTTGAATTT GCTCTGCGGG ACTCAAACCA ACCTAATCAC 
CCGCAGGAAG CCTTAAATGC ATTTCACGAA GAAGCTGTGC AAAATCAAGT TATGGGAATG 
ACCATTGCAT CAATTAATGG TCCTTCTCCT TTAAAATGGA ATGTACCTGC TACTAAAGAT 
ATGCACACAT TATCACAGTC GGTGAATGCT GCTAATGACC CACCAACATG TATAAACCAA 
AAGACAGATG CTGGAATAGC TGAGGTTTTA AATGGATTTA CAAAGGCTGA AGCCAATATG 
TGCCATTCAG TATCTGTCCT TAGTACTTGT GCACCTTTAG GCTCACCTCG TTTAAGGCTT 
AGTGCTCTAA AGGAGGGACA TAAACAGAGT GATATACGGA GTGCCTCCTG CAGTAGTGCT 
TGTCTTGAAA AAGTAGCATC AGAAACAGCT CTTGCCGCCA TGTCTGACAG CAACATGAAG 
AAAGAGGATA GTTTCAACAT GGCATTTGAA CTTTTACCGT CGTCTCCTCC ATTGCATTCA 
TTTTTTGGGG CTTCACAAAA TGTGCACACA TCTGAAGAAT CATCCTCTTT GCTTGGAACA 
GTGGGTCAAA CAAAAGAAAA TACCATAAGT GTTAAATCAA TTGGATCCAG CAATGTTGTT 
CTTAATGAAG GAAAGGGGGA AACCTCCCAG TTTACGATAT TGTCCAAATC ACACAGTGAG 
GTAGAAGATG TTCAGAAAGT GTGTGTACGT TTGCCCTCTT CCCCACTGCT TGAAACAGCT 
TTACAAATGA AAGCGTATGC CCACAGTGAC AAGCTCAGTG CCTTCAACAA TAATCCATGT 
CTTGAGAAAG TATTACCAGA GGCAACCCCA GTGGTTTTAT CAGAAACATA TAGGGAGGTA 
CAAGATTGTT TTGGATCTTT ACCTTCATCT GATTTGCATG AAAATGAGCC TCCACTGGAA 
GAGAGATCCC TGGTTGATAA ATCTAATAGC TCCAGAAATT ATACTTGCCT TGAGAAAGTA 
GTACCAGAGG CAACCTTATT GTTAAAAGAG TACTTTTCAG AATCCTGTAG TAAGGTACAA 
GATGATGAGA AATGTTTTGG ATCTTTACCT TCATCTGCTT TGCAAAGAAA AAATCCTCTA 
ATTGAAAAAA ACGGACCGAG TGACAAATCC AGTGCATCCA GCAATAATGT TTGCCTTGAG 
GAAGGAGAAC CAGAGGCAGT CCCATTGTTG GAAGACTGTA AAAGTCAAAC CCTTGTTGTA 
CCTTCCACAT GCAGTCAATT GTCCATAACA GATAAAATAG CAAACAGTCT TGGAGTACAT 
AATTCCTCAG ATTCATGTCA GCTCATCAGT GAAATTCATC ATGATCATCC AAGCGATGGA 
AATGATCTCA CTGTGTCCCA TATTGCCTTC AATGTTGGTG GTGCTGACAA AATCAATAAA 
GTTAAAAGCC TGCAGGTAAC TCCACTTCCA GAGGTTATAT CTACAGAACT ATGTAGGGGC 
AAGAGCAACA GTCAAAGCAC TTGTGAACCT CCATCCGTGC CAGTTCTGTC ATGTAAGCTT 
TCTTCTGAGC AAAAAGATGA AGGTACATTG ATGGAATTGT CTAGCTCTTT ACATAGTGAA 
ACAACTGAGG AGGAAGAATG TGGAAATAAA ATGGCTCTTG GAAGTTCAAC ATTGGATGGG 
GAAATATCTG ACTATAAGAA ACTAAACAGT CAAATTAAGC CTTTGCCACA AGCAATAGAA 
AGCTTGGTCA TGGACACGTG TGATAGCAAT CTGTGCAATA ATATTAATTT AAAAGATAGC 
AGCAAAATAA TTTCAGAGGA AGCTACAGTA CCACATTGTT TTCAGCCAGA ACAGCATGTT 
GTATACAGCC ATCCTATGTC TGACCCTGCA GTTACTTGTG TGACTCATGG CACTGGATCT 
GAAATGAAAG CATTGGATAC ACCAAGGAAG GAGTCTAATC CAGTGTGTAA GGAACTCCGT 
GAAAGAAATG TAAATCCAAT GAAAGAATTG TATATATCTC TGAACAATGC ACCAACTGAT 
TTGTCTAACT CTTTGTGCAG TGACACTACT AATGTGGAAG AATCTAGGGA TGCCACAGCA 
AATGCAAGTT CTTTATCCAA CGGAGAAATA TTGCATTTAT ATCACAAAAC AAGCAGCCCA 
GCTCTTGTTG ATCCAAAAGC AGAAGCAAAT ACAGTTTTAG GCTTGTGTGA CAGTGTCCTC 
AGCAAAAACT CTACAATGGG AAGTAGTGAT AGCATTAATC AGGAGAAAGC TATAGCAACA 
ACTTTTGCAC ACCCAGGACA GAGTGTGTTG TTCAGCCATG GGGCATCTAC AATTACAGTC 
TCCCCTGGTT TATTTCATAG TGCTGTACAG GCATTAGATG TTTCTGATAC ACCAGAGTTG 
GGGTGCAGTC CTGTGTGTAA GGAACCCACT GTTGTACAAG ATAAAAGCGA TGGATCCTTT 
GATACTTCTT TGGGCCATTT TGAAAGAGAA AAGCAAAATG AGAGCTTTGA AACAGACAGT 
TCTGTAATGC TTACTGATGA TCACATAAAG GATCCAAAGG ATGTAGATGT TGTATTGTCT 
TTGCCAGAGG GGAGCTTGGA AGATTGCTGT CCCAAAAAAA AATCAAACTG CTCAGAGAAC 
ATGCATGTAG CCTTAAAGGG TACCTCCACA AACCCTTCCC TTGAACTCGG TTCCCACGAT 
GCCCCGTGTG AAAAGAAGCT GCTTCAATGG AGAAACACCC TCTTCCTCTG CTGGTTGGAC 
TGCATATTGG CAGCACTGGT GCATTCTGCT AGCCTGAATA GCATTGTGGC ACAGCGAAGG 
GTTGCAGAAA GCTCAATCAT TCACCTTCTT CTGGAGAAAT ACAAGGAGGC CAATGCATTA 
ATAAAAAGCA CATCCAGACG AGGACGTCCA AAGAATTTAT CTCAAGCTGA AAACATCCTG 
AATAAAGTGC GAATGACCAT CTTTGATGAA ATAAAGCCCT TGTTAAAGTG TGAATTGGGA 
GATAAAGAGA GCCCTGTGTT TGCTTTGCCG TTACTGCTGC AGCTGGTTCC AGAGATAGAA 
TCCTGTTTTG TGCACTCAGG TGTATGGGAG TTTACATGCA AGCTATGTGG CTACCAATAC 
CAAAATAAGC ACCAACGGAC TGTGTCCACA TTCACTAAGA TCTTACCTGA ATGGACACCC 
CTAAATGCTA TCCATAAAGC TCCTTGCAAC AAATGCCTGG GTACAGATCA GATACGTGCT 
TTTCGTATTA AAAAGATCCA TGACCTATTC ATACTTCACT TTGTGGAAGG CCTGCCAAGC 
AATGATTTAG GCATGTACTC GTTTCACTTT GAAGGCTGCC TGTATGAAGT AAAGACTGTC 
ATCCAGTATA GTGAAGACCA TTTCTCATCT TGGATTGCTA ATGAGGATGG CACATGGCTG 
GAATCTGATG ACCTGAAAGG GTATTTCTGC ATAAAACAGA ACTGTTTTGA GGTTCCTGCT 
TCAGAAATTC ATATTGTAAT ATGGGAAAAG AAGATTACCA ACAGCACTGG AGCACACCAT 
GCAATGGAAC CTGAAACAAA AAACAAAGCA CTTAATTCTT TATCTACTTC ACCTTCATCT 
TTATCTCAGA CCCTGCACCT GTCTCCTTCA GCTGCTCCGA ATGCTGCATC TTCACCTCTA 
AGAGATGCCC CAAATGCTTC CAGCGTACTC TCTGGAATGG AGGGTTTTCA TGATGACGAC 
ATCATAACGC TCACTCTCAT TGAAATTCCA ATTGATGCAT CGGGTAATGT GATTGAGAAC 
AACTCTGAGG CAGCCCAGCA GCCAAGTCTC ATTGCTGAAA CACATTCATC CCCTGTAACC 
CTCAAAGCAG TTGCTGATGG GCAATGTGAT CAAAATCTAT GTTCTGGTAC AATGAAGGAA 
AGCTCTTTCG CTGGTGGTTC TCTTCCAGTT CCTAACATTT CCAGCCCTCC TGCCCCTAAC 
ATTTCCAGCC CTCCTGCCCC TAACATTTCC AGCCCTCCTG CCCCTAACAT TTCCAGCCCT 
CCTGCCTCTA ACATTTCCAG CCCTCCTGCC CCTCACATTT CCAGCCCTCC TGCCCCTCAC 
ATTTCCAGCC CTCCTGCCCC TCACATTTCC AGCCCTCCTG CCCCTCACAT TTCCAGCCCT 
CCTGCCCCTC ACATTTCCAG CCCTCCTGCC CCTCACATTT CCAGCCCTCC TGCCCCTAAC 
AGCGCCAGTC CCCCTGCCCC TAACAGCGCC AGTACTCCCC TCCCTAACAG TTCCAGTTCA 
AAAAAAGCCT TTGTTGGAAG TTGGATGAAG AGTCTACTAA ATAAAAACCC TTTGTTCCTG 
ACATCTAATG TGCCCTGTGC AAATAAGAAA ACTGTGGCAA AAACTCCAAT CCGATTTCCC 
CTGCTGAAGA CAACAGATTT GCAGGCATCT ACTAAACAGG CCCAGAGCTT TGATGGTTTT 
AAAGCTAAAC GTGTCGGTAA TTCATTCAGC TCGGACAATT GCCTTGGTGT AGGTCCCACG 
AAGGATAATT CCACTGCACC CAAACCTGCC ATCTCTAGCC AAACGTTCCA GCCACTGGTT 
TCAAATCGAC TTAAAAATGG GCGGTTAGGA CTCGGTAATA CCAAAGACTT GGCAAAAAAT 
GTTTCTCTGT CTAAGGAGGA TAAGGTGCTT AGGCTTAGAC TGAAAATGCT GAAAAAGTTG 
AAGGCTAAGA AACATGAATT GGCCGCCATA GATATGCTTG GAAAGTCACA GGCTGGTAGT 
GATCTGGAGC GTAGCAGCAC CAGCAGGCGT GAACAATTAC GCGGGTTCTT GCAGGAACTC 
CAAGAGCAAA TCGATAATGC CGACACTGAA TCAGTGTGCA CTATGAGCTC TTGCACGTCG 
ATATGCAGCA GCCCAGGAGA TGCGGAATTC TTTGCGGAAT TGTTCTCCCC ACCCCCTAGC 
AACGCCCCGC AGGAGAGCAT GGATGACAGT AATTTTTTAG AGATGTATGT AGATGGTTAC 
AACCCCTCCT GTGGCAGTTA TGGAACCAGT AACCCAAGTG GCAAAGAGGT CTCACAGGAC 
AGTGCTAATA TCGGCCAGTC CAATTTACAG CGTGCTTCCG ACAGCCACAA TAACACTAAC 
GTGGGGGACG TCTTCCATGA TATGCTGTCA ACCTCCACAA TGCACATGCT GAACGAGGAT 
TATTTCTCAC CTTTTGATGA TATGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947220.1
CDS	2802..8591
Translation	MTKVSQWFTTMASETPGSTVSLVGQGSILDKSSLHTVGCLGKNVDPCAAPADGFCPVCKAKGQLQALRTYRFNFTQSIYLCANPQCIYPLGYTPLDNIIANTADLQKHHSPGKQKKRCLLDTSVTTNCVKKHKTDSFVSGEYISENLFLSAQNKGYKSMSPCLPKESHTSVPGPSENGQDERSQQQYGMCKVSACNSASPHGDRAPSVEFALRDSNQPNHPQEALNAFHEEAVQNQVMGMTIASINGPSPLKWNVPATKDMHTLSQSVNAANDPPTCINQKTDAGIAEVLNGFTKAEANMCHSVSVLSTCAPLGSPRLRLSALKEGHKQSDIRSASCSSACLEKVASETALAAMSDSNMKKEDSFNMAFELLPSSPPLHSFFGASQNVHTSEESSSLLGTVGQTKENTISVKSIGSSNVVLNEGKGETSQFTILSKSHSEVEDVQKVCVRLPSSPLLETALQMKAYAHSDKLSAFNNNPCLEKVLPEATPVVLSETYREVQDCFGSLPSSDLHENEPPLEERSLVDKSNSSRNYTCLEKVVPEATLLLKEYFSESCSKVQDDEKCFGSLPSSALQRKNPLIEKNGPSDKSSASSNNVCLEEGEPEAVPLLEDCKSQTLVVPSTCSQLSITDKIANSLGVHNSSDSCQLISEIHHDHPSDGNDLTVSHIAFNVGGADKINKVKSLQVTPLPEVISTELCRGKSNSQSTCEPPSVPVLSCKLSSEQKDEGTLMELSSSLHSETTEEEECGNKMALGSSTLDGEISDYKKLNSQIKPLPQAIESLVMDTCDSNLCNNINLKDSSKIISEEATVPHCFQPEQHVVYSHPMSDPAVTCVTHGTGSEMKALDTPRKESNPVCKELRERNVNPMKELYISLNNAPTDLSNSLCSDTTNVEESRDATANASSLSNGEILHLYHKTSSPALVDPKAEANTVLGLCDSVLSKNSTMGSSDSINQEKAIATTFAHPGQSVLFSHGASTITVSPGLFHSAVQALDVSDTPELGCSPVCKEPTVVQDKSDGSFDTSLGHFEREKQNESFETDSSVMLTDDHIKDPKDVDVVLSLPEGSLEDCCPKKKSNCSENMHVALKGTSTNPSLELGSHDAPCEKKLLQWRNTLFLCWLDCILAALVHSASLNSIVAQRRVAESSIIHLLLEKYKEANALIKSTSRRGRPKNLSQAENILNKVRMTIFDEIKPLLKCELGDKESPVFALPLLLQLVPEIESCFVHSGVWEFTCKLCGYQYQNKHQRTVSTFTKILPEWTPLNAIHKAPCNKCLGTDQIRAFRIKKIHDLFILHFVEGLPSNDLGMYSFHFEGCLYEVKTVIQYSEDHFSSWIANEDGTWLESDDLKGYFCIKQNCFEVPASEIHIVIWEKKITNSTGAHHAMEPETKNKALNSLSTSPSSLSQTLHLSPSAAPNAASSPLRDAPNASSVLSGMEGFHDDDIITLTLIEIPIDASGNVIENNSEAAQQPSLIAETHSSPVTLKAVADGQCDQNLCSGTMKESSFAGGSLPVPNISSPPAPNISSPPAPNISSPPAPNISSPPASNISSPPAPHISSPPAPHISSPPAPHISSPPAPHISSPPAPHISSPPAPHISSPPAPNSASPPAPNSASTPLPNSSSSKKAFVGSWMKSLLNKNPLFLTSNVPCANKKTVAKTPIRFPLLKTTDLQASTKQAQSFDGFKAKRVGNSFSSDNCLGVGPTKDNSTAPKPAISSQTFQPLVSNRLKNGRLGLGNTKDLAKNVSLSKEDKVLRLRLKMLKKLKAKKHELAAIDMLGKSQAGSDLERSSTSRREQLRGFLQELQEQIDNADTESVCTMSSCTSICSSPGDAEFFAELFSPPPSNAPQESMDDSNFLEMYVDGYNPSCGSYGTSNPSGKEVSQDSANIGQSNLQRASDSHNNTNVGDVFHDMLSTSTMHMLNEDYFSPFDDMF

Target ORF information:

RefSeq Version	XM_018091731.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin specific peptidase like 1 (uspl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018091731.1

ORF Insert Sequence:

ATGACTAAAG TAAGCCAATG GTTCACAACG ATGGCTTCAG AGACTCCTGG ATCTACAGTG 
TCACTGGTTG GACAAGGGTC TATTTTAGAT AAATCTTCAC TCCACACGGT GGGGTGCTTG 
GGAAAAAATG TTGACCCCTG TGCAGCTCCT GCTGATGGAT TTTGCCCAGT ATGCAAGGCC 
AAAGGCCAGC TGCAAGCTCT CCGGACATAT CGCTTTAATT TCACTCAGTC TATATATCTC 
TGTGCCAATC CACAGTGCAT ATATCCCTTG GGATATACAC CTCTGGATAA CATAATTGCA 
AACACAGCAG ATCTACAGAA ACACCATAGC CCTGGAAAGC AGAAGAAGCG GTGTCTTTTA 
GACACCTCTG TAACAACTAA CTGTGTGAAG AAGCATAAGA CTGATTCTTT TGTCTCTGGA 
GAGTACATTT CAGAAAACCT ATTTTTATCT GCACAAAATA AAGGCTACAA ATCAATGTCT 
CCGTGTTTAC CTAAAGAATC TCATACAAGT GTACCTGGCC CTTCAGAGAA TGGTCAGGAT 
GAGAGAAGTC AGCAGCAATA TGGAATGTGC AAAGTTTCAG CTTGCAATTC TGCAAGTCCA 
CATGGTGACA GGGCACCTTC AGTTGAATTT GCTCTGCGGG ACTCAAACCA ACCTAATCAC 
CCGCAGGAAG CCTTAAATGC ATTTCACGAA GAAGCTGTGC AAAATCAAGT TATGGGAATG 
ACCATTGCAT CAATTAATGG TCCTTCTCCT TTAAAATGGA ATGTACCTGC TACTAAAGAT 
ATGCACACAT TATCACAGTC GGTGAATGCT GCTAATGACC CACCAACATG TATAAACCAA 
AAGACAGATG CTGGAATAGC TGAGGTTTTA AATGGATTTA CAAAGGCTGA AGCCAATATG 
TGCCATTCAG TATCTGTCCT TAGTACTTGT GCACCTTTAG GCTCACCTCG TTTAAGGCTT 
AGTGCTCTAA AGGAGGGACA TAAACAGAGT GATATACGGA GTGCCTCCTG CAGTAGTGCT 
TGTCTTGAAA AAGTAGCATC AGAAACAGCT CTTGCCGCCA TGTCTGACAG CAACATGAAG 
AAAGAGGATA GTTTCAACAT GGCATTTGAA CTTTTACCGT CGTCTCCTCC ATTGCATTCA 
TTTTTTGGGG CTTCACAAAA TGTGCACACA TCTGAAGAAT CATCCTCTTT GCTTGGAACA 
GTGGGTCAAA CAAAAGAAAA TACCATAAGT GTTAAATCAA TTGGATCCAG CAATGTTGTT 
CTTAATGAAG GAAAGGGGGA AACCTCCCAG TTTACGATAT TGTCCAAATC ACACAGTGAG 
GTAGAAGATG TTCAGAAAGT GTGTGTACGT TTGCCCTCTT CCCCACTGCT TGAAACAGCT 
TTACAAATGA AAGCGTATGC CCACAGTGAC AAGCTCAGTG CCTTCAACAA TAATCCATGT 
CTTGAGAAAG TATTACCAGA GGCAACCCCA GTGGTTTTAT CAGAAACATA TAGGGAGGTA 
CAAGATTGTT TTGGATCTTT ACCTTCATCT GATTTGCATG AAAATGAGCC TCCACTGGAA 
GAGAGATCCC TGGTTGATAA ATCTAATAGC TCCAGAAATT ATACTTGCCT TGAGAAAGTA 
GTACCAGAGG CAACCTTATT GTTAAAAGAG TACTTTTCAG AATCCTGTAG TAAGGTACAA 
GATGATGAGA AATGTTTTGG ATCTTTACCT TCATCTGCTT TGCAAAGAAA AAATCCTCTA 
ATTGAAAAAA ACGGACCGAG TGACAAATCC AGTGCATCCA GCAATAATGT TTGCCTTGAG 
GAAGGAGAAC CAGAGGCAGT CCCATTGTTG GAAGACTGTA AAAGTCAAAC CCTTGTTGTA 
CCTTCCACAT GCAGTCAATT GTCCATAACA GATAAAATAG CAAACAGTCT TGGAGTACAT 
AATTCCTCAG ATTCATGTCA GCTCATCAGT GAAATTCATC ATGATCATCC AAGCGATGGA 
AATGATCTCA CTGTGTCCCA TATTGCCTTC AATGTTGGTG GTGCTGACAA AATCAATAAA 
GTTAAAAGCC TGCAGGTAAC TCCACTTCCA GAGGTTATAT CTACAGAACT ATGTAGGGGC 
AAGAGCAACA GTCAAAGCAC TTGTGAACCT CCATCCGTGC CAGTTCTGTC ATGTAAGCTT 
TCTTCTGAGC AAAAAGATGA AGGTACATTG ATGGAATTGT CTAGCTCTTT ACATAGTGAA 
ACAACTGAGG AGGAAGAATG TGGAAATAAA ATGGCTCTTG GAAGTTCAAC ATTGGATGGG 
GAAATATCTG ACTATAAGAA ACTAAACAGT CAAATTAAGC CTTTGCCACA AGCAATAGAA 
AGCTTGGTCA TGGACACGTG TGATAGCAAT CTGTGCAATA ATATTAATTT AAAAGATAGC 
AGCAAAATAA TTTCAGAGGA AGCTACAGTA CCACATTGTT TTCAGCCAGA ACAGCATGTT 
GTATACAGCC ATCCTATGTC TGACCCTGCA GTTACTTGTG TGACTCATGG CACTGGATCT 
GAAATGAAAG CATTGGATAC ACCAAGGAAG GAGTCTAATC CAGTGTGTAA GGAACTCCGT 
GAAAGAAATG TAAATCCAAT GAAAGAATTG TATATATCTC TGAACAATGC ACCAACTGAT 
TTGTCTAACT CTTTGTGCAG TGACACTACT AATGTGGAAG AATCTAGGGA TGCCACAGCA 
AATGCAAGTT CTTTATCCAA CGGAGAAATA TTGCATTTAT ATCACAAAAC AAGCAGCCCA 
GCTCTTGTTG ATCCAAAAGC AGAAGCAAAT ACAGTTTTAG GCTTGTGTGA CAGTGTCCTC 
AGCAAAAACT CTACAATGGG AAGTAGTGAT AGCATTAATC AGGAGAAAGC TATAGCAACA 
ACTTTTGCAC ACCCAGGACA GAGTGTGTTG TTCAGCCATG GGGCATCTAC AATTACAGTC 
TCCCCTGGTT TATTTCATAG TGCTGTACAG GCATTAGATG TTTCTGATAC ACCAGAGTTG 
GGGTGCAGTC CTGTGTGTAA GGAACCCACT GTTGTACAAG ATAAAAGCGA TGGATCCTTT 
GATACTTCTT TGGGCCATTT TGAAAGAGAA AAGCAAAATG AGAGCTTTGA AACAGACAGT 
TCTGTAATGC TTACTGATGA TCACATAAAG GATCCAAAGG ATGTAGATGT TGTATTGTCT 
TTGCCAGAGG GGAGCTTGGA AGATTGCTGT CCCAAAAAAA AATCAAACTG CTCAGAGAAC 
ATGCATGTAG CCTTAAAGGG TACCTCCACA AACCCTTCCC TTGAACTCGG TTCCCACGAT 
GCCCCGTGTG AAAAGAAGCT GCTTCAATGG AGAAACACCC TCTTCCTCTG CTGGTTGGAC 
TGCATATTGG CAGCACTGGT GCATTCTGCT AGCCTGAATA GCATTGTGGC ACAGCGAAGG 
GTTGCAGAAA GCTCAATCAT TCACCTTCTT CTGGAGAAAT ACAAGGAGGC CAATGCATTA 
ATAAAAAGCA CATCCAGACG AGGACGTCCA AAGAATTTAT CTCAAGCTGA AAACATCCTG 
AATAAAGTGC GAATGACCAT CTTTGATGAA ATAAAGCCCT TGTTAAAGTG TGAATTGGGA 
GATAAAGAGA GCCCTGTGTT TGCTTTGCCG TTACTGCTGC AGCTGGTTCC AGAGATAGAA 
TCCTGTTTTG TGCACTCAGG TGTATGGGAG TTTACATGCA AGCTATGTGG CTACCAATAC 
CAAAATAAGC ACCAACGGAC TGTGTCCACA TTCACTAAGA TCTTACCTGA ATGGACACCC 
CTAAATGCTA TCCATAAAGC TCCTTGCAAC AAATGCCTGG GTACAGATCA GATACGTGCT 
TTTCGTATTA AAAAGATCCA TGACCTATTC ATACTTCACT TTGTGGAAGG CCTGCCAAGC 
AATGATTTAG GCATGTACTC GTTTCACTTT GAAGGCTGCC TGTATGAAGT AAAGACTGTC 
ATCCAGTATA GTGAAGACCA TTTCTCATCT TGGATTGCTA ATGAGGATGG CACATGGCTG 
GAATCTGATG ACCTGAAAGG GTATTTCTGC ATAAAACAGA ACTGTTTTGA GGTTCCTGCT 
TCAGAAATTC ATATTGTAAT ATGGGAAAAG AAGATTACCA ACAGCACTGG AGCACACCAT 
GCAATGGAAC CTGAAACAAA AAACAAAGCA CTTAATTCTT TATCTACTTC ACCTTCATCT 
TTATCTCAGA CCCTGCACCT GTCTCCTTCA GCTGCTCCGA ATGCTGCATC TTCACCTCTA 
AGAGATGCCC CAAATGCTTC CAGCGTACTC TCTGGAATGG AGGGTTTTCA TGATGACGAC 
ATCATAACGC TCACTCTCAT TGAAATTCCA ATTGATGCAT CGGGTAATGT GATTGAGAAC 
AACTCTGAGG CAGCCCAGCA GCCAAGTCTC ATTGCTGAAA CACATTCATC CCCTGTAACC 
CTCAAAGCAG TTGCTGATGG GCAATGTGAT CAAAATCTAT GTTCTGGTAC AATGAAGGAA 
AGCTCTTTCG CTGGTGGTTC TCTTCCAGTT CCTAACATTT CCAGCCCTCC TGCCCCTAAC 
ATTTCCAGCC CTCCTGCCCC TAACATTTCC AGCCCTCCTG CCCCTAACAT TTCCAGCCCT 
CCTGCCTCTA ACATTTCCAG CCCTCCTGCC CCTCACATTT CCAGCCCTCC TGCCCCTCAC 
ATTTCCAGCC CTCCTGCCCC TCACATTTCC AGCCCTCCTG CCCCTCACAT TTCCAGCCCT 
CCTGCCCCTC ACATTTCCAG CCCTCCTGCC CCTCACATTT CCAGCCCTCC TGCCCCTAAC 
AGCGCCAGTC CCCCTGCCCC TAACAGCGCC AGTACTCCCC TCCCTAACAG TTCCAGTTCA 
AAAAAAGCCT TTGTTGGAAG TTGGATGAAG AGTCTACTAA ATAAAAACCC TTTGTTCCTG 
ACATCTAATG TGCCCTGTGC AAATAAGAAA ACTGTGGCAA AAACTCCAAT CCGATTTCCC 
CTGCTGAAGA CAACAGATTT GCAGGCATCT ACTAAACAGG CCCAGAGCTT TGATGGTTTT 
AAAGCTAAAC GTGTCGGTAA TTCATTCAGC TCGGACAATT GCCTTGGTGT AGGTCCCACG 
AAGGATAATT CCACTGCACC CAAACCTGCC ATCTCTAGCC AAACGTTCCA GCCACTGGTT 
TCAAATCGAC TTAAAAATGG GCGGTTAGGA CTCGGTAATA CCAAAGACTT GGCAAAAAAT 
GTTTCTCTGT CTAAGGAGGA TAAGGTGCTT AGGCTTAGAC TGAAAATGCT GAAAAAGTTG 
AAGGCTAAGA AACATGAATT GGCCGCCATA GATATGCTTG GAAAGTCACA GGCTGGTAGT 
GATCTGGAGC GTAGCAGCAC CAGCAGGCGT GAACAATTAC GCGGGTTCTT GCAGGAACTC 
CAAGAGCAAA TCGATAATGC CGACACTGAA TCAGTGTGCA CTATGAGCTC TTGCACGTCG 
ATATGCAGCA GCCCAGGAGA TGCGGAATTC TTTGCGGAAT TGTTCTCCCC ACCCCCTAGC 
AACGCCCCGC AGGAGAGCAT GGATGACAGT AATTTTTTAG AGATGTATGT AGATGGTTAC 
AACCCCTCCT GTGGCAGTTA TGGAACCAGT AACCCAAGTG GCAAAGAGGT CTCACAGGAC 
AGTGCTAATA TCGGCCAGTC CAATTTACAG CGTGCTTCCG ACAGCCACAA TAACACTAAC 
GTGGGGGACG TCTTCCATGA TATGCTGTCA ACCTCCACAA TGCACATGCT GAACGAGGAT 
TATTTCTCAC CTTTTGATGA TATGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

uspl1 ( XM_018091732.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947221.1 Xenopus tropicalis ubiquitin specific peptidase like 1 (uspl1), transcript variant X2, mRNA.

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CloneID	OXa42866
Clone ID Related Accession (Same CDS sequence)	XM_018091733.1 , XM_018091731.1 , XM_018091732.1
Accession Version	XM_018091732.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5790bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	SUMO-specific isopeptidase USPL1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTAAAG TAAGCCAATG GTTCACAACG ATGGCTTCAG AGACTCCTGG ATCTACAGTG 
TCACTGGTTG GACAAGGGTC TATTTTAGAT AAATCTTCAC TCCACACGGT GGGGTGCTTG 
GGAAAAAATG TTGACCCCTG TGCAGCTCCT GCTGATGGAT TTTGCCCAGT ATGCAAGGCC 
AAAGGCCAGC TGCAAGCTCT CCGGACATAT CGCTTTAATT TCACTCAGTC TATATATCTC 
TGTGCCAATC CACAGTGCAT ATATCCCTTG GGATATACAC CTCTGGATAA CATAATTGCA 
AACACAGCAG ATCTACAGAA ACACCATAGC CCTGGAAAGC AGAAGAAGCG GTGTCTTTTA 
GACACCTCTG TAACAACTAA CTGTGTGAAG AAGCATAAGA CTGATTCTTT TGTCTCTGGA 
GAGTACATTT CAGAAAACCT ATTTTTATCT GCACAAAATA AAGGCTACAA ATCAATGTCT 
CCGTGTTTAC CTAAAGAATC TCATACAAGT GTACCTGGCC CTTCAGAGAA TGGTCAGGAT 
GAGAGAAGTC AGCAGCAATA TGGAATGTGC AAAGTTTCAG CTTGCAATTC TGCAAGTCCA 
CATGGTGACA GGGCACCTTC AGTTGAATTT GCTCTGCGGG ACTCAAACCA ACCTAATCAC 
CCGCAGGAAG CCTTAAATGC ATTTCACGAA GAAGCTGTGC AAAATCAAGT TATGGGAATG 
ACCATTGCAT CAATTAATGG TCCTTCTCCT TTAAAATGGA ATGTACCTGC TACTAAAGAT 
ATGCACACAT TATCACAGTC GGTGAATGCT GCTAATGACC CACCAACATG TATAAACCAA 
AAGACAGATG CTGGAATAGC TGAGGTTTTA AATGGATTTA CAAAGGCTGA AGCCAATATG 
TGCCATTCAG TATCTGTCCT TAGTACTTGT GCACCTTTAG GCTCACCTCG TTTAAGGCTT 
AGTGCTCTAA AGGAGGGACA TAAACAGAGT GATATACGGA GTGCCTCCTG CAGTAGTGCT 
TGTCTTGAAA AAGTAGCATC AGAAACAGCT CTTGCCGCCA TGTCTGACAG CAACATGAAG 
AAAGAGGATA GTTTCAACAT GGCATTTGAA CTTTTACCGT CGTCTCCTCC ATTGCATTCA 
TTTTTTGGGG CTTCACAAAA TGTGCACACA TCTGAAGAAT CATCCTCTTT GCTTGGAACA 
GTGGGTCAAA CAAAAGAAAA TACCATAAGT GTTAAATCAA TTGGATCCAG CAATGTTGTT 
CTTAATGAAG GAAAGGGGGA AACCTCCCAG TTTACGATAT TGTCCAAATC ACACAGTGAG 
GTAGAAGATG TTCAGAAAGT GTGTGTACGT TTGCCCTCTT CCCCACTGCT TGAAACAGCT 
TTACAAATGA AAGCGTATGC CCACAGTGAC AAGCTCAGTG CCTTCAACAA TAATCCATGT 
CTTGAGAAAG TATTACCAGA GGCAACCCCA GTGGTTTTAT CAGAAACATA TAGGGAGGTA 
CAAGATTGTT TTGGATCTTT ACCTTCATCT GATTTGCATG AAAATGAGCC TCCACTGGAA 
GAGAGATCCC TGGTTGATAA ATCTAATAGC TCCAGAAATT ATACTTGCCT TGAGAAAGTA 
GTACCAGAGG CAACCTTATT GTTAAAAGAG TACTTTTCAG AATCCTGTAG TAAGGTACAA 
GATGATGAGA AATGTTTTGG ATCTTTACCT TCATCTGCTT TGCAAAGAAA AAATCCTCTA 
ATTGAAAAAA ACGGACCGAG TGACAAATCC AGTGCATCCA GCAATAATGT TTGCCTTGAG 
GAAGGAGAAC CAGAGGCAGT CCCATTGTTG GAAGACTGTA AAAGTCAAAC CCTTGTTGTA 
CCTTCCACAT GCAGTCAATT GTCCATAACA GATAAAATAG CAAACAGTCT TGGAGTACAT 
AATTCCTCAG ATTCATGTCA GCTCATCAGT GAAATTCATC ATGATCATCC AAGCGATGGA 
AATGATCTCA CTGTGTCCCA TATTGCCTTC AATGTTGGTG GTGCTGACAA AATCAATAAA 
GTTAAAAGCC TGCAGGTAAC TCCACTTCCA GAGGTTATAT CTACAGAACT ATGTAGGGGC 
AAGAGCAACA GTCAAAGCAC TTGTGAACCT CCATCCGTGC CAGTTCTGTC ATGTAAGCTT 
TCTTCTGAGC AAAAAGATGA AGGTACATTG ATGGAATTGT CTAGCTCTTT ACATAGTGAA 
ACAACTGAGG AGGAAGAATG TGGAAATAAA ATGGCTCTTG GAAGTTCAAC ATTGGATGGG 
GAAATATCTG ACTATAAGAA ACTAAACAGT CAAATTAAGC CTTTGCCACA AGCAATAGAA 
AGCTTGGTCA TGGACACGTG TGATAGCAAT CTGTGCAATA ATATTAATTT AAAAGATAGC 
AGCAAAATAA TTTCAGAGGA AGCTACAGTA CCACATTGTT TTCAGCCAGA ACAGCATGTT 
GTATACAGCC ATCCTATGTC TGACCCTGCA GTTACTTGTG TGACTCATGG CACTGGATCT 
GAAATGAAAG CATTGGATAC ACCAAGGAAG GAGTCTAATC CAGTGTGTAA GGAACTCCGT 
GAAAGAAATG TAAATCCAAT GAAAGAATTG TATATATCTC TGAACAATGC ACCAACTGAT 
TTGTCTAACT CTTTGTGCAG TGACACTACT AATGTGGAAG AATCTAGGGA TGCCACAGCA 
AATGCAAGTT CTTTATCCAA CGGAGAAATA TTGCATTTAT ATCACAAAAC AAGCAGCCCA 
GCTCTTGTTG ATCCAAAAGC AGAAGCAAAT ACAGTTTTAG GCTTGTGTGA CAGTGTCCTC 
AGCAAAAACT CTACAATGGG AAGTAGTGAT AGCATTAATC AGGAGAAAGC TATAGCAACA 
ACTTTTGCAC ACCCAGGACA GAGTGTGTTG TTCAGCCATG GGGCATCTAC AATTACAGTC 
TCCCCTGGTT TATTTCATAG TGCTGTACAG GCATTAGATG TTTCTGATAC ACCAGAGTTG 
GGGTGCAGTC CTGTGTGTAA GGAACCCACT GTTGTACAAG ATAAAAGCGA TGGATCCTTT 
GATACTTCTT TGGGCCATTT TGAAAGAGAA AAGCAAAATG AGAGCTTTGA AACAGACAGT 
TCTGTAATGC TTACTGATGA TCACATAAAG GATCCAAAGG ATGTAGATGT TGTATTGTCT 
TTGCCAGAGG GGAGCTTGGA AGATTGCTGT CCCAAAAAAA AATCAAACTG CTCAGAGAAC 
ATGCATGTAG CCTTAAAGGG TACCTCCACA AACCCTTCCC TTGAACTCGG TTCCCACGAT 
GCCCCGTGTG AAAAGAAGCT GCTTCAATGG AGAAACACCC TCTTCCTCTG CTGGTTGGAC 
TGCATATTGG CAGCACTGGT GCATTCTGCT AGCCTGAATA GCATTGTGGC ACAGCGAAGG 
GTTGCAGAAA GCTCAATCAT TCACCTTCTT CTGGAGAAAT ACAAGGAGGC CAATGCATTA 
ATAAAAAGCA CATCCAGACG AGGACGTCCA AAGAATTTAT CTCAAGCTGA AAACATCCTG 
AATAAAGTGC GAATGACCAT CTTTGATGAA ATAAAGCCCT TGTTAAAGTG TGAATTGGGA 
GATAAAGAGA GCCCTGTGTT TGCTTTGCCG TTACTGCTGC AGCTGGTTCC AGAGATAGAA 
TCCTGTTTTG TGCACTCAGG TGTATGGGAG TTTACATGCA AGCTATGTGG CTACCAATAC 
CAAAATAAGC ACCAACGGAC TGTGTCCACA TTCACTAAGA TCTTACCTGA ATGGACACCC 
CTAAATGCTA TCCATAAAGC TCCTTGCAAC AAATGCCTGG GTACAGATCA GATACGTGCT 
TTTCGTATTA AAAAGATCCA TGACCTATTC ATACTTCACT TTGTGGAAGG CCTGCCAAGC 
AATGATTTAG GCATGTACTC GTTTCACTTT GAAGGCTGCC TGTATGAAGT AAAGACTGTC 
ATCCAGTATA GTGAAGACCA TTTCTCATCT TGGATTGCTA ATGAGGATGG CACATGGCTG 
GAATCTGATG ACCTGAAAGG GTATTTCTGC ATAAAACAGA ACTGTTTTGA GGTTCCTGCT 
TCAGAAATTC ATATTGTAAT ATGGGAAAAG AAGATTACCA ACAGCACTGG AGCACACCAT 
GCAATGGAAC CTGAAACAAA AAACAAAGCA CTTAATTCTT TATCTACTTC ACCTTCATCT 
TTATCTCAGA CCCTGCACCT GTCTCCTTCA GCTGCTCCGA ATGCTGCATC TTCACCTCTA 
AGAGATGCCC CAAATGCTTC CAGCGTACTC TCTGGAATGG AGGGTTTTCA TGATGACGAC 
ATCATAACGC TCACTCTCAT TGAAATTCCA ATTGATGCAT CGGGTAATGT GATTGAGAAC 
AACTCTGAGG CAGCCCAGCA GCCAAGTCTC ATTGCTGAAA CACATTCATC CCCTGTAACC 
CTCAAAGCAG TTGCTGATGG GCAATGTGAT CAAAATCTAT GTTCTGGTAC AATGAAGGAA 
AGCTCTTTCG CTGGTGGTTC TCTTCCAGTT CCTAACATTT CCAGCCCTCC TGCCCCTAAC 
ATTTCCAGCC CTCCTGCCCC TAACATTTCC AGCCCTCCTG CCCCTAACAT TTCCAGCCCT 
CCTGCCTCTA ACATTTCCAG CCCTCCTGCC CCTCACATTT CCAGCCCTCC TGCCCCTCAC 
ATTTCCAGCC CTCCTGCCCC TCACATTTCC AGCCCTCCTG CCCCTCACAT TTCCAGCCCT 
CCTGCCCCTC ACATTTCCAG CCCTCCTGCC CCTCACATTT CCAGCCCTCC TGCCCCTAAC 
AGCGCCAGTC CCCCTGCCCC TAACAGCGCC AGTACTCCCC TCCCTAACAG TTCCAGTTCA 
AAAAAAGCCT TTGTTGGAAG TTGGATGAAG AGTCTACTAA ATAAAAACCC TTTGTTCCTG 
ACATCTAATG TGCCCTGTGC AAATAAGAAA ACTGTGGCAA AAACTCCAAT CCGATTTCCC 
CTGCTGAAGA CAACAGATTT GCAGGCATCT ACTAAACAGG CCCAGAGCTT TGATGGTTTT 
AAAGCTAAAC GTGTCGGTAA TTCATTCAGC TCGGACAATT GCCTTGGTGT AGGTCCCACG 
AAGGATAATT CCACTGCACC CAAACCTGCC ATCTCTAGCC AAACGTTCCA GCCACTGGTT 
TCAAATCGAC TTAAAAATGG GCGGTTAGGA CTCGGTAATA CCAAAGACTT GGCAAAAAAT 
GTTTCTCTGT CTAAGGAGGA TAAGGTGCTT AGGCTTAGAC TGAAAATGCT GAAAAAGTTG 
AAGGCTAAGA AACATGAATT GGCCGCCATA GATATGCTTG GAAAGTCACA GGCTGGTAGT 
GATCTGGAGC GTAGCAGCAC CAGCAGGCGT GAACAATTAC GCGGGTTCTT GCAGGAACTC 
CAAGAGCAAA TCGATAATGC CGACACTGAA TCAGTGTGCA CTATGAGCTC TTGCACGTCG 
ATATGCAGCA GCCCAGGAGA TGCGGAATTC TTTGCGGAAT TGTTCTCCCC ACCCCCTAGC 
AACGCCCCGC AGGAGAGCAT GGATGACAGT AATTTTTTAG AGATGTATGT AGATGGTTAC 
AACCCCTCCT GTGGCAGTTA TGGAACCAGT AACCCAAGTG GCAAAGAGGT CTCACAGGAC 
AGTGCTAATA TCGGCCAGTC CAATTTACAG CGTGCTTCCG ACAGCCACAA TAACACTAAC 
GTGGGGGACG TCTTCCATGA TATGCTGTCA ACCTCCACAA TGCACATGCT GAACGAGGAT 
TATTTCTCAC CTTTTGATGA TATGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947221.1
CDS	2422..8211
Translation	MTKVSQWFTTMASETPGSTVSLVGQGSILDKSSLHTVGCLGKNVDPCAAPADGFCPVCKAKGQLQALRTYRFNFTQSIYLCANPQCIYPLGYTPLDNIIANTADLQKHHSPGKQKKRCLLDTSVTTNCVKKHKTDSFVSGEYISENLFLSAQNKGYKSMSPCLPKESHTSVPGPSENGQDERSQQQYGMCKVSACNSASPHGDRAPSVEFALRDSNQPNHPQEALNAFHEEAVQNQVMGMTIASINGPSPLKWNVPATKDMHTLSQSVNAANDPPTCINQKTDAGIAEVLNGFTKAEANMCHSVSVLSTCAPLGSPRLRLSALKEGHKQSDIRSASCSSACLEKVASETALAAMSDSNMKKEDSFNMAFELLPSSPPLHSFFGASQNVHTSEESSSLLGTVGQTKENTISVKSIGSSNVVLNEGKGETSQFTILSKSHSEVEDVQKVCVRLPSSPLLETALQMKAYAHSDKLSAFNNNPCLEKVLPEATPVVLSETYREVQDCFGSLPSSDLHENEPPLEERSLVDKSNSSRNYTCLEKVVPEATLLLKEYFSESCSKVQDDEKCFGSLPSSALQRKNPLIEKNGPSDKSSASSNNVCLEEGEPEAVPLLEDCKSQTLVVPSTCSQLSITDKIANSLGVHNSSDSCQLISEIHHDHPSDGNDLTVSHIAFNVGGADKINKVKSLQVTPLPEVISTELCRGKSNSQSTCEPPSVPVLSCKLSSEQKDEGTLMELSSSLHSETTEEEECGNKMALGSSTLDGEISDYKKLNSQIKPLPQAIESLVMDTCDSNLCNNINLKDSSKIISEEATVPHCFQPEQHVVYSHPMSDPAVTCVTHGTGSEMKALDTPRKESNPVCKELRERNVNPMKELYISLNNAPTDLSNSLCSDTTNVEESRDATANASSLSNGEILHLYHKTSSPALVDPKAEANTVLGLCDSVLSKNSTMGSSDSINQEKAIATTFAHPGQSVLFSHGASTITVSPGLFHSAVQALDVSDTPELGCSPVCKEPTVVQDKSDGSFDTSLGHFEREKQNESFETDSSVMLTDDHIKDPKDVDVVLSLPEGSLEDCCPKKKSNCSENMHVALKGTSTNPSLELGSHDAPCEKKLLQWRNTLFLCWLDCILAALVHSASLNSIVAQRRVAESSIIHLLLEKYKEANALIKSTSRRGRPKNLSQAENILNKVRMTIFDEIKPLLKCELGDKESPVFALPLLLQLVPEIESCFVHSGVWEFTCKLCGYQYQNKHQRTVSTFTKILPEWTPLNAIHKAPCNKCLGTDQIRAFRIKKIHDLFILHFVEGLPSNDLGMYSFHFEGCLYEVKTVIQYSEDHFSSWIANEDGTWLESDDLKGYFCIKQNCFEVPASEIHIVIWEKKITNSTGAHHAMEPETKNKALNSLSTSPSSLSQTLHLSPSAAPNAASSPLRDAPNASSVLSGMEGFHDDDIITLTLIEIPIDASGNVIENNSEAAQQPSLIAETHSSPVTLKAVADGQCDQNLCSGTMKESSFAGGSLPVPNISSPPAPNISSPPAPNISSPPAPNISSPPASNISSPPAPHISSPPAPHISSPPAPHISSPPAPHISSPPAPHISSPPAPHISSPPAPNSASPPAPNSASTPLPNSSSSKKAFVGSWMKSLLNKNPLFLTSNVPCANKKTVAKTPIRFPLLKTTDLQASTKQAQSFDGFKAKRVGNSFSSDNCLGVGPTKDNSTAPKPAISSQTFQPLVSNRLKNGRLGLGNTKDLAKNVSLSKEDKVLRLRLKMLKKLKAKKHELAAIDMLGKSQAGSDLERSSTSRREQLRGFLQELQEQIDNADTESVCTMSSCTSICSSPGDAEFFAELFSPPPSNAPQESMDDSNFLEMYVDGYNPSCGSYGTSNPSGKEVSQDSANIGQSNLQRASDSHNNTNVGDVFHDMLSTSTMHMLNEDYFSPFDDMF

Target ORF information:

RefSeq Version	XM_018091732.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin specific peptidase like 1 (uspl1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018091732.1

ORF Insert Sequence:

ATGACTAAAG TAAGCCAATG GTTCACAACG ATGGCTTCAG AGACTCCTGG ATCTACAGTG 
TCACTGGTTG GACAAGGGTC TATTTTAGAT AAATCTTCAC TCCACACGGT GGGGTGCTTG 
GGAAAAAATG TTGACCCCTG TGCAGCTCCT GCTGATGGAT TTTGCCCAGT ATGCAAGGCC 
AAAGGCCAGC TGCAAGCTCT CCGGACATAT CGCTTTAATT TCACTCAGTC TATATATCTC 
TGTGCCAATC CACAGTGCAT ATATCCCTTG GGATATACAC CTCTGGATAA CATAATTGCA 
AACACAGCAG ATCTACAGAA ACACCATAGC CCTGGAAAGC AGAAGAAGCG GTGTCTTTTA 
GACACCTCTG TAACAACTAA CTGTGTGAAG AAGCATAAGA CTGATTCTTT TGTCTCTGGA 
GAGTACATTT CAGAAAACCT ATTTTTATCT GCACAAAATA AAGGCTACAA ATCAATGTCT 
CCGTGTTTAC CTAAAGAATC TCATACAAGT GTACCTGGCC CTTCAGAGAA TGGTCAGGAT 
GAGAGAAGTC AGCAGCAATA TGGAATGTGC AAAGTTTCAG CTTGCAATTC TGCAAGTCCA 
CATGGTGACA GGGCACCTTC AGTTGAATTT GCTCTGCGGG ACTCAAACCA ACCTAATCAC 
CCGCAGGAAG CCTTAAATGC ATTTCACGAA GAAGCTGTGC AAAATCAAGT TATGGGAATG 
ACCATTGCAT CAATTAATGG TCCTTCTCCT TTAAAATGGA ATGTACCTGC TACTAAAGAT 
ATGCACACAT TATCACAGTC GGTGAATGCT GCTAATGACC CACCAACATG TATAAACCAA 
AAGACAGATG CTGGAATAGC TGAGGTTTTA AATGGATTTA CAAAGGCTGA AGCCAATATG 
TGCCATTCAG TATCTGTCCT TAGTACTTGT GCACCTTTAG GCTCACCTCG TTTAAGGCTT 
AGTGCTCTAA AGGAGGGACA TAAACAGAGT GATATACGGA GTGCCTCCTG CAGTAGTGCT 
TGTCTTGAAA AAGTAGCATC AGAAACAGCT CTTGCCGCCA TGTCTGACAG CAACATGAAG 
AAAGAGGATA GTTTCAACAT GGCATTTGAA CTTTTACCGT CGTCTCCTCC ATTGCATTCA 
TTTTTTGGGG CTTCACAAAA TGTGCACACA TCTGAAGAAT CATCCTCTTT GCTTGGAACA 
GTGGGTCAAA CAAAAGAAAA TACCATAAGT GTTAAATCAA TTGGATCCAG CAATGTTGTT 
CTTAATGAAG GAAAGGGGGA AACCTCCCAG TTTACGATAT TGTCCAAATC ACACAGTGAG 
GTAGAAGATG TTCAGAAAGT GTGTGTACGT TTGCCCTCTT CCCCACTGCT TGAAACAGCT 
TTACAAATGA AAGCGTATGC CCACAGTGAC AAGCTCAGTG CCTTCAACAA TAATCCATGT 
CTTGAGAAAG TATTACCAGA GGCAACCCCA GTGGTTTTAT CAGAAACATA TAGGGAGGTA 
CAAGATTGTT TTGGATCTTT ACCTTCATCT GATTTGCATG AAAATGAGCC TCCACTGGAA 
GAGAGATCCC TGGTTGATAA ATCTAATAGC TCCAGAAATT ATACTTGCCT TGAGAAAGTA 
GTACCAGAGG CAACCTTATT GTTAAAAGAG TACTTTTCAG AATCCTGTAG TAAGGTACAA 
GATGATGAGA AATGTTTTGG ATCTTTACCT TCATCTGCTT TGCAAAGAAA AAATCCTCTA 
ATTGAAAAAA ACGGACCGAG TGACAAATCC AGTGCATCCA GCAATAATGT TTGCCTTGAG 
GAAGGAGAAC CAGAGGCAGT CCCATTGTTG GAAGACTGTA AAAGTCAAAC CCTTGTTGTA 
CCTTCCACAT GCAGTCAATT GTCCATAACA GATAAAATAG CAAACAGTCT TGGAGTACAT 
AATTCCTCAG ATTCATGTCA GCTCATCAGT GAAATTCATC ATGATCATCC AAGCGATGGA 
AATGATCTCA CTGTGTCCCA TATTGCCTTC AATGTTGGTG GTGCTGACAA AATCAATAAA 
GTTAAAAGCC TGCAGGTAAC TCCACTTCCA GAGGTTATAT CTACAGAACT ATGTAGGGGC 
AAGAGCAACA GTCAAAGCAC TTGTGAACCT CCATCCGTGC CAGTTCTGTC ATGTAAGCTT 
TCTTCTGAGC AAAAAGATGA AGGTACATTG ATGGAATTGT CTAGCTCTTT ACATAGTGAA 
ACAACTGAGG AGGAAGAATG TGGAAATAAA ATGGCTCTTG GAAGTTCAAC ATTGGATGGG 
GAAATATCTG ACTATAAGAA ACTAAACAGT CAAATTAAGC CTTTGCCACA AGCAATAGAA 
AGCTTGGTCA TGGACACGTG TGATAGCAAT CTGTGCAATA ATATTAATTT AAAAGATAGC 
AGCAAAATAA TTTCAGAGGA AGCTACAGTA CCACATTGTT TTCAGCCAGA ACAGCATGTT 
GTATACAGCC ATCCTATGTC TGACCCTGCA GTTACTTGTG TGACTCATGG CACTGGATCT 
GAAATGAAAG CATTGGATAC ACCAAGGAAG GAGTCTAATC CAGTGTGTAA GGAACTCCGT 
GAAAGAAATG TAAATCCAAT GAAAGAATTG TATATATCTC TGAACAATGC ACCAACTGAT 
TTGTCTAACT CTTTGTGCAG TGACACTACT AATGTGGAAG AATCTAGGGA TGCCACAGCA 
AATGCAAGTT CTTTATCCAA CGGAGAAATA TTGCATTTAT ATCACAAAAC AAGCAGCCCA 
GCTCTTGTTG ATCCAAAAGC AGAAGCAAAT ACAGTTTTAG GCTTGTGTGA CAGTGTCCTC 
AGCAAAAACT CTACAATGGG AAGTAGTGAT AGCATTAATC AGGAGAAAGC TATAGCAACA 
ACTTTTGCAC ACCCAGGACA GAGTGTGTTG TTCAGCCATG GGGCATCTAC AATTACAGTC 
TCCCCTGGTT TATTTCATAG TGCTGTACAG GCATTAGATG TTTCTGATAC ACCAGAGTTG 
GGGTGCAGTC CTGTGTGTAA GGAACCCACT GTTGTACAAG ATAAAAGCGA TGGATCCTTT 
GATACTTCTT TGGGCCATTT TGAAAGAGAA AAGCAAAATG AGAGCTTTGA AACAGACAGT 
TCTGTAATGC TTACTGATGA TCACATAAAG GATCCAAAGG ATGTAGATGT TGTATTGTCT 
TTGCCAGAGG GGAGCTTGGA AGATTGCTGT CCCAAAAAAA AATCAAACTG CTCAGAGAAC 
ATGCATGTAG CCTTAAAGGG TACCTCCACA AACCCTTCCC TTGAACTCGG TTCCCACGAT 
GCCCCGTGTG AAAAGAAGCT GCTTCAATGG AGAAACACCC TCTTCCTCTG CTGGTTGGAC 
TGCATATTGG CAGCACTGGT GCATTCTGCT AGCCTGAATA GCATTGTGGC ACAGCGAAGG 
GTTGCAGAAA GCTCAATCAT TCACCTTCTT CTGGAGAAAT ACAAGGAGGC CAATGCATTA 
ATAAAAAGCA CATCCAGACG AGGACGTCCA AAGAATTTAT CTCAAGCTGA AAACATCCTG 
AATAAAGTGC GAATGACCAT CTTTGATGAA ATAAAGCCCT TGTTAAAGTG TGAATTGGGA 
GATAAAGAGA GCCCTGTGTT TGCTTTGCCG TTACTGCTGC AGCTGGTTCC AGAGATAGAA 
TCCTGTTTTG TGCACTCAGG TGTATGGGAG TTTACATGCA AGCTATGTGG CTACCAATAC 
CAAAATAAGC ACCAACGGAC TGTGTCCACA TTCACTAAGA TCTTACCTGA ATGGACACCC 
CTAAATGCTA TCCATAAAGC TCCTTGCAAC AAATGCCTGG GTACAGATCA GATACGTGCT 
TTTCGTATTA AAAAGATCCA TGACCTATTC ATACTTCACT TTGTGGAAGG CCTGCCAAGC 
AATGATTTAG GCATGTACTC GTTTCACTTT GAAGGCTGCC TGTATGAAGT AAAGACTGTC 
ATCCAGTATA GTGAAGACCA TTTCTCATCT TGGATTGCTA ATGAGGATGG CACATGGCTG 
GAATCTGATG ACCTGAAAGG GTATTTCTGC ATAAAACAGA ACTGTTTTGA GGTTCCTGCT 
TCAGAAATTC ATATTGTAAT ATGGGAAAAG AAGATTACCA ACAGCACTGG AGCACACCAT 
GCAATGGAAC CTGAAACAAA AAACAAAGCA CTTAATTCTT TATCTACTTC ACCTTCATCT 
TTATCTCAGA CCCTGCACCT GTCTCCTTCA GCTGCTCCGA ATGCTGCATC TTCACCTCTA 
AGAGATGCCC CAAATGCTTC CAGCGTACTC TCTGGAATGG AGGGTTTTCA TGATGACGAC 
ATCATAACGC TCACTCTCAT TGAAATTCCA ATTGATGCAT CGGGTAATGT GATTGAGAAC 
AACTCTGAGG CAGCCCAGCA GCCAAGTCTC ATTGCTGAAA CACATTCATC CCCTGTAACC 
CTCAAAGCAG TTGCTGATGG GCAATGTGAT CAAAATCTAT GTTCTGGTAC AATGAAGGAA 
AGCTCTTTCG CTGGTGGTTC TCTTCCAGTT CCTAACATTT CCAGCCCTCC TGCCCCTAAC 
ATTTCCAGCC CTCCTGCCCC TAACATTTCC AGCCCTCCTG CCCCTAACAT TTCCAGCCCT 
CCTGCCTCTA ACATTTCCAG CCCTCCTGCC CCTCACATTT CCAGCCCTCC TGCCCCTCAC 
ATTTCCAGCC CTCCTGCCCC TCACATTTCC AGCCCTCCTG CCCCTCACAT TTCCAGCCCT 
CCTGCCCCTC ACATTTCCAG CCCTCCTGCC CCTCACATTT CCAGCCCTCC TGCCCCTAAC 
AGCGCCAGTC CCCCTGCCCC TAACAGCGCC AGTACTCCCC TCCCTAACAG TTCCAGTTCA 
AAAAAAGCCT TTGTTGGAAG TTGGATGAAG AGTCTACTAA ATAAAAACCC TTTGTTCCTG 
ACATCTAATG TGCCCTGTGC AAATAAGAAA ACTGTGGCAA AAACTCCAAT CCGATTTCCC 
CTGCTGAAGA CAACAGATTT GCAGGCATCT ACTAAACAGG CCCAGAGCTT TGATGGTTTT 
AAAGCTAAAC GTGTCGGTAA TTCATTCAGC TCGGACAATT GCCTTGGTGT AGGTCCCACG 
AAGGATAATT CCACTGCACC CAAACCTGCC ATCTCTAGCC AAACGTTCCA GCCACTGGTT 
TCAAATCGAC TTAAAAATGG GCGGTTAGGA CTCGGTAATA CCAAAGACTT GGCAAAAAAT 
GTTTCTCTGT CTAAGGAGGA TAAGGTGCTT AGGCTTAGAC TGAAAATGCT GAAAAAGTTG 
AAGGCTAAGA AACATGAATT GGCCGCCATA GATATGCTTG GAAAGTCACA GGCTGGTAGT 
GATCTGGAGC GTAGCAGCAC CAGCAGGCGT GAACAATTAC GCGGGTTCTT GCAGGAACTC 
CAAGAGCAAA TCGATAATGC CGACACTGAA TCAGTGTGCA CTATGAGCTC TTGCACGTCG 
ATATGCAGCA GCCCAGGAGA TGCGGAATTC TTTGCGGAAT TGTTCTCCCC ACCCCCTAGC 
AACGCCCCGC AGGAGAGCAT GGATGACAGT AATTTTTTAG AGATGTATGT AGATGGTTAC 
AACCCCTCCT GTGGCAGTTA TGGAACCAGT AACCCAAGTG GCAAAGAGGT CTCACAGGAC 
AGTGCTAATA TCGGCCAGTC CAATTTACAG CGTGCTTCCG ACAGCCACAA TAACACTAAC 
GTGGGGGACG TCTTCCATGA TATGCTGTCA ACCTCCACAA TGCACATGCT GAACGAGGAT 
TATTTCTCAC CTTTTGATGA TATGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

uspl1 ( XM_018091734.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947223.1 Xenopus tropicalis ubiquitin specific peptidase like 1 (uspl1), transcript variant X4, mRNA.

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CloneID	OXa42867
Clone ID Related Accession (Same CDS sequence)	XM_018091734.1
Accession Version	XM_018091734.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	SUMO-specific isopeptidase USPL1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCCGT GTTTACCTAA AGAATCTCAT ACAAGTGTAC CTGGCCCTTC AGAGAATGGT 
CAGGATGAGA GAAGTCAGCA GCAATATGGA ATGTGCAAAG TTTCAGCTTG CAATTCTGCA 
AGTCCACATG GTGACAGGGC ACCTTCAGTT GAATTTGCTC TGCGGGACTC AAACCAACCT 
AATCACCCGC AGGAAGCCTT AAATGCATTT CACGAAGAAG CTGTGCAAAA TCAAGTTATG 
GGAATGACCA TTGCATCAAT TAATGGTCCT TCTCCTTTAA AATGGAATGT ACCTGCTACT 
AAAGATATGC ACACATTATC ACAGTCGGTG AATGCTGCTA ATGACCCACC AACATGTATA 
AACCAAAAGA CAGATGCTGG AATAGCTGAG GTTTTAAATG GATTTACAAA GGCTGAAGCC 
AATATGTGCC ATTCAGTATC TGTCCTTAGT ACTTGTGCAC CTTTAGGCTC ACCTCGTTTA 
AGGCTTAGTG CTCTAAAGGA GGGACATAAA CAGAGTGATA TACGGAGTGC CTCCTGCAGT 
AGTGCTTGTC TTGAAAAAGT AGCATCAGAA ACAGCTCTTG CCGCCATGTC TGACAGCAAC 
ATGAAGAAAG AGGATAGTTT CAACATGGCA TTTGAACTTT TACCGTCGTC TCCTCCATTG 
CATTCATTTT TTGGGGCTTC ACAAAATGTG CACACATCTG AAGAATCATC CTCTTTGCTT 
GGAACAGTGG GTCAAACAAA AGAAAATACC ATAAGTGTTA AATCAATTGG ATCCAGCAAT 
GTTGTTCTTA ATGAAGGAAA GGGGGAAACC TCCCAGTTTA CGATATTGTC CAAATCACAC 
AGTGAGGTAG AAGATGTTCA GAAAGTGTGT GTACGTTTGC CCTCTTCCCC ACTGCTTGAA 
ACAGCTTTAC AAATGAAAGC GTATGCCCAC AGTGACAAGC TCAGTGCCTT CAACAATAAT 
CCATGTCTTG AGAAAGTATT ACCAGAGGCA ACCCCAGTGG TTTTATCAGA AACATATAGG 
GAGGTACAAG ATTGTTTTGG ATCTTTACCT TCATCTGATT TGCATGAAAA TGAGCCTCCA 
CTGGAAGAGA GATCCCTGGT TGATAAATCT AATAGCTCCA GAAATTATAC TTGCCTTGAG 
AAAGTAGTAC CAGAGGCAAC CTTATTGTTA AAAGAGTACT TTTCAGAATC CTGTAGTAAG 
GTACAAGATG ATGAGAAATG TTTTGGATCT TTACCTTCAT CTGCTTTGCA AAGAAAAAAT 
CCTCTAATTG AAAAAAACGG ACCGAGTGAC AAATCCAGTG CATCCAGCAA TAATGTTTGC 
CTTGAGGAAG GAGAACCAGA GGCAGTCCCA TTGTTGGAAG ACTGTAAAAG TCAAACCCTT 
GTTGTACCTT CCACATGCAG TCAATTGTCC ATAACAGATA AAATAGCAAA CAGTCTTGGA 
GTACATAATT CCTCAGATTC ATGTCAGCTC ATCAGTGAAA TTCATCATGA TCATCCAAGC 
GATGGAAATG ATCTCACTGT GTCCCATATT GCCTTCAATG TTGGTGGTGC TGACAAAATC 
AATAAAGTTA AAAGCCTGCA GGTAACTCCA CTTCCAGAGG TTATATCTAC AGAACTATGT 
AGGGGCAAGA GCAACAGTCA AAGCACTTGT GAACCTCCAT CCGTGCCAGT TCTGTCATGT 
AAGCTTTCTT CTGAGCAAAA AGATGAAGGT ACATTGATGG AATTGTCTAG CTCTTTACAT 
AGTGAAACAA CTGAGGAGGA AGAATGTGGA AATAAAATGG CTCTTGGAAG TTCAACATTG 
GATGGGGAAA TATCTGACTA TAAGAAACTA AACAGTCAAA TTAAGCCTTT GCCACAAGCA 
ATAGAAAGCT TGGTCATGGA CACGTGTGAT AGCAATCTGT GCAATAATAT TAATTTAAAA 
GATAGCAGCA AAATAATTTC AGAGGAAGCT ACAGTACCAC ATTGTTTTCA GCCAGAACAG 
CATGTTGTAT ACAGCCATCC TATGTCTGAC CCTGCAGTTA CTTGTGTGAC TCATGGCACT 
GGATCTGAAA TGAAAGCATT GGATACACCA AGGAAGGAGT CTAATCCAGT GTGTAAGGAA 
CTCCGTGAAA GAAATGTAAA TCCAATGAAA GAATTGTATA TATCTCTGAA CAATGCACCA 
ACTGATTTGT CTAACTCTTT GTGCAGTGAC ACTACTAATG TGGAAGAATC TAGGGATGCC 
ACAGCAAATG CAAGTTCTTT ATCCAACGGA GAAATATTGC ATTTATATCA CAAAACAAGC 
AGCCCAGCTC TTGTTGATCC AAAAGCAGAA GCAAATACAG TTTTAGGCTT GTGTGACAGT 
GTCCTCAGCA AAAACTCTAC AATGGGAAGT AGTGATAGCA TTAATCAGGA GAAAGCTATA 
GCAACAACTT TTGCACACCC AGGACAGAGT GTGTTGTTCA GCCATGGGGC ATCTACAATT 
ACAGTCTCCC CTGGTTTATT TCATAGTGCT GTACAGGCAT TAGATGTTTC TGATACACCA 
GAGTTGGGGT GCAGTCCTGT GTGTAAGGAA CCCACTGTTG TACAAGATAA AAGCGATGGA 
TCCTTTGATA CTTCTTTGGG CCATTTTGAA AGAGAAAAGC AAAATGAGAG CTTTGAAACA 
GACAGTTCTG TAATGCTTAC TGATGATCAC ATAAAGGATC CAAAGGATGT AGATGTTGTA 
TTGTCTTTGC CAGAGGGGAG CTTGGAAGAT TGCTGTCCCA AAAAAAAATC AAACTGCTCA 
GAGAACATGC ATGTAGCCTT AAAGGGTACC TCCACAAACC CTTCCCTTGA ACTCGGTTCC 
CACGATGCCC CGTGTGAAAA GAAGCTGCTT CAATGGAGAA ACACCCTCTT CCTCTGCTGG 
TTGGACTGCA TATTGGCAGC ACTGGTGCAT TCTGCTAGCC TGAATAGCAT TGTGGCACAG 
CGAAGGGTTG CAGAAAGCTC AATCATTCAC CTTCTTCTGG AGAAATACAA GGAGGCCAAT 
GCATTAATAA AAAGCACATC CAGACGAGGA CGTCCAAAGA ATTTATCTCA AGCTGAAAAC 
ATCCTGAATA AAGTGCGAAT GACCATCTTT GATGAAATAA AGCCCTTGTT AAAGTGTGAA 
TTGGGAGATA AAGAGAGCCC TGTGTTTGCT TTGCCGTTAC TGCTGCAGCT GGTTCCAGAG 
ATAGAATCCT GTTTTGTGCA CTCAGGTGTA TGGGAGTTTA CATGCAAGCT ATGTGGCTAC 
CAATACCAAA ATAAGCACCA ACGGACTGTG TCCACATTCA CTAAGATCTT ACCTGAATGG 
ACACCCCTAA ATGCTATCCA TAAAGCTCCT TGCAACAAAT GCCTGGGTAC AGATCAGATA 
CGTGCTTTTC GTATTAAAAA GATCCATGAC CTATTCATAC TTCACTTTGT GGAAGGCCTG 
CCAAGCAATG ATTTAGGCAT GTACTCGTTT CACTTTGAAG GCTGCCTGTA TGAAGTAAAG 
ACTGTCATCC AGTATAGTGA AGACCATTTC TCATCTTGGA TTGCTAATGA GGATGGCACA 
TGGCTGGAAT CTGATGACCT GAAAGGGTAT TTCTGCATAA AACAGAACTG TTTTGAGGTT 
CCTGCTTCAG AAATTCATAT TGTAATATGG GAAAAGAAGA TTACCAACAG CACTGGAGCA 
CACCATGCAA TGGAACCTGA AACAAAAAAC AAAGCACTTA ATTCTTTATC TACTTCACCT 
TCATCTTTAT CTCAGACCCT GCACCTGTCT CCTTCAGCTG CTCCGAATGC TGCATCTTCA 
CCTCTAAGAG ATGCCCCAAA TGCTTCCAGC GTACTCTCTG GAATGGAGGG TTTTCATGAT 
GACGACATCA TAACGCTCAC TCTCATTGAA ATTCCAATTG ATGCATCGGG TAATGTGATT 
GAGAACAACT CTGAGGCAGC CCAGCAGCCA AGTCTCATTG CTGAAACACA TTCATCCCCT 
GTAACCCTCA AAGCAGTTGC TGATGGGCAA TGTGATCAAA ATCTATGTTC TGGTACAATG 
AAGGAAAGCT CTTTCGCTGG TGGTTCTCTT CCAGTTCCTA ACATTTCCAG CCCTCCTGCC 
CCTAACATTT CCAGCCCTCC TGCCCCTAAC ATTTCCAGCC CTCCTGCCCC TAACATTTCC 
AGCCCTCCTG CCTCTAACAT TTCCAGCCCT CCTGCCCCTC ACATTTCCAG CCCTCCTGCC 
CCTCACATTT CCAGCCCTCC TGCCCCTCAC ATTTCCAGCC CTCCTGCCCC TCACATTTCC 
AGCCCTCCTG CCCCTCACAT TTCCAGCCCT CCTGCCCCTC ACATTTCCAG CCCTCCTGCC 
CCTAACAGCG CCAGTCCCCC TGCCCCTAAC AGCGCCAGTA CTCCCCTCCC TAACAGTTCC 
AGTTCAAAAA AAGCCTTTGT TGGAAGTTGG ATGAAGAGTC TACTAAATAA AAACCCTTTG 
TTCCTGACAT CTAATGTGCC CTGTGCAAAT AAGAAAACTG TGGCAAAAAC TCCAATCCGA 
TTTCCCCTGC TGAAGACAAC AGATTTGCAG GCATCTACTA AACAGGCCCA GAGCTTTGAT 
GGTTTTAAAG CTAAACGTGT CGGTAATTCA TTCAGCTCGG ACAATTGCCT TGGTGTAGGT 
CCCACGAAGG ATAATTCCAC TGCACCCAAA CCTGCCATCT CTAGCCAAAC GTTCCAGCCA 
CTGGTTTCAA ATCGACTTAA AAATGGGCGG TTAGGACTCG GTAATACCAA AGACTTGGCA 
AAAAATGTTT CTCTGTCTAA GGAGGATAAG GTGCTTAGGC TTAGACTGAA AATGCTGAAA 
AAGTTGAAGG CTAAGAAACA TGAATTGGCC GCCATAGATA TGCTTGGAAA GTCACAGGCT 
GGTAGTGATC TGGAGCGTAG CAGCACCAGC AGGCGTGAAC AATTACGCGG GTTCTTGCAG 
GAACTCCAAG AGCAAATCGA TAATGCCGAC ACTGAATCAG TGTGCACTAT GAGCTCTTGC 
ACGTCGATAT GCAGCAGCCC AGGAGATGCG GAATTCTTTG CGGAATTGTT CTCCCCACCC 
CCTAGCAACG CCCCGCAGGA GAGCATGGAT GACAGTAATT TTTTAGAGAT GTATGTAGAT 
GGTTACAACC CCTCCTGTGG CAGTTATGGA ACCAGTAACC CAAGTGGCAA AGAGGTCTCA 
CAGGACAGTG CTAATATCGG CCAGTCCAAT TTACAGCGTG CTTCCGACAG CCACAATAAC 
ACTAACGTGG GGGACGTCTT CCATGATATG CTGTCAACCT CCACAATGCA CATGCTGAAC 
GAGGATTATT TCTCACCTTT TGATGATATG TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947223.1
CDS	829..6144
Translation	MSPCLPKESHTSVPGPSENGQDERSQQQYGMCKVSACNSASPHGDRAPSVEFALRDSNQPNHPQEALNAFHEEAVQNQVMGMTIASINGPSPLKWNVPATKDMHTLSQSVNAANDPPTCINQKTDAGIAEVLNGFTKAEANMCHSVSVLSTCAPLGSPRLRLSALKEGHKQSDIRSASCSSACLEKVASETALAAMSDSNMKKEDSFNMAFELLPSSPPLHSFFGASQNVHTSEESSSLLGTVGQTKENTISVKSIGSSNVVLNEGKGETSQFTILSKSHSEVEDVQKVCVRLPSSPLLETALQMKAYAHSDKLSAFNNNPCLEKVLPEATPVVLSETYREVQDCFGSLPSSDLHENEPPLEERSLVDKSNSSRNYTCLEKVVPEATLLLKEYFSESCSKVQDDEKCFGSLPSSALQRKNPLIEKNGPSDKSSASSNNVCLEEGEPEAVPLLEDCKSQTLVVPSTCSQLSITDKIANSLGVHNSSDSCQLISEIHHDHPSDGNDLTVSHIAFNVGGADKINKVKSLQVTPLPEVISTELCRGKSNSQSTCEPPSVPVLSCKLSSEQKDEGTLMELSSSLHSETTEEEECGNKMALGSSTLDGEISDYKKLNSQIKPLPQAIESLVMDTCDSNLCNNINLKDSSKIISEEATVPHCFQPEQHVVYSHPMSDPAVTCVTHGTGSEMKALDTPRKESNPVCKELRERNVNPMKELYISLNNAPTDLSNSLCSDTTNVEESRDATANASSLSNGEILHLYHKTSSPALVDPKAEANTVLGLCDSVLSKNSTMGSSDSINQEKAIATTFAHPGQSVLFSHGASTITVSPGLFHSAVQALDVSDTPELGCSPVCKEPTVVQDKSDGSFDTSLGHFEREKQNESFETDSSVMLTDDHIKDPKDVDVVLSLPEGSLEDCCPKKKSNCSENMHVALKGTSTNPSLELGSHDAPCEKKLLQWRNTLFLCWLDCILAALVHSASLNSIVAQRRVAESSIIHLLLEKYKEANALIKSTSRRGRPKNLSQAENILNKVRMTIFDEIKPLLKCELGDKESPVFALPLLLQLVPEIESCFVHSGVWEFTCKLCGYQYQNKHQRTVSTFTKILPEWTPLNAIHKAPCNKCLGTDQIRAFRIKKIHDLFILHFVEGLPSNDLGMYSFHFEGCLYEVKTVIQYSEDHFSSWIANEDGTWLESDDLKGYFCIKQNCFEVPASEIHIVIWEKKITNSTGAHHAMEPETKNKALNSLSTSPSSLSQTLHLSPSAAPNAASSPLRDAPNASSVLSGMEGFHDDDIITLTLIEIPIDASGNVIENNSEAAQQPSLIAETHSSPVTLKAVADGQCDQNLCSGTMKESSFAGGSLPVPNISSPPAPNISSPPAPNISSPPAPNISSPPASNISSPPAPHISSPPAPHISSPPAPHISSPPAPHISSPPAPHISSPPAPHISSPPAPNSASPPAPNSASTPLPNSSSSKKAFVGSWMKSLLNKNPLFLTSNVPCANKKTVAKTPIRFPLLKTTDLQASTKQAQSFDGFKAKRVGNSFSSDNCLGVGPTKDNSTAPKPAISSQTFQPLVSNRLKNGRLGLGNTKDLAKNVSLSKEDKVLRLRLKMLKKLKAKKHELAAIDMLGKSQAGSDLERSSTSRREQLRGFLQELQEQIDNADTESVCTMSSCTSICSSPGDAEFFAELFSPPPSNAPQESMDDSNFLEMYVDGYNPSCGSYGTSNPSGKEVSQDSANIGQSNLQRASDSHNNTNVGDVFHDMLSTSTMHMLNEDYFSPFDDMF

Target ORF information:

RefSeq Version	XM_018091734.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin specific peptidase like 1 (uspl1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018091734.1

ORF Insert Sequence:

ATGTCTCCGT GTTTACCTAA AGAATCTCAT ACAAGTGTAC CTGGCCCTTC AGAGAATGGT 
CAGGATGAGA GAAGTCAGCA GCAATATGGA ATGTGCAAAG TTTCAGCTTG CAATTCTGCA 
AGTCCACATG GTGACAGGGC ACCTTCAGTT GAATTTGCTC TGCGGGACTC AAACCAACCT 
AATCACCCGC AGGAAGCCTT AAATGCATTT CACGAAGAAG CTGTGCAAAA TCAAGTTATG 
GGAATGACCA TTGCATCAAT TAATGGTCCT TCTCCTTTAA AATGGAATGT ACCTGCTACT 
AAAGATATGC ACACATTATC ACAGTCGGTG AATGCTGCTA ATGACCCACC AACATGTATA 
AACCAAAAGA CAGATGCTGG AATAGCTGAG GTTTTAAATG GATTTACAAA GGCTGAAGCC 
AATATGTGCC ATTCAGTATC TGTCCTTAGT ACTTGTGCAC CTTTAGGCTC ACCTCGTTTA 
AGGCTTAGTG CTCTAAAGGA GGGACATAAA CAGAGTGATA TACGGAGTGC CTCCTGCAGT 
AGTGCTTGTC TTGAAAAAGT AGCATCAGAA ACAGCTCTTG CCGCCATGTC TGACAGCAAC 
ATGAAGAAAG AGGATAGTTT CAACATGGCA TTTGAACTTT TACCGTCGTC TCCTCCATTG 
CATTCATTTT TTGGGGCTTC ACAAAATGTG CACACATCTG AAGAATCATC CTCTTTGCTT 
GGAACAGTGG GTCAAACAAA AGAAAATACC ATAAGTGTTA AATCAATTGG ATCCAGCAAT 
GTTGTTCTTA ATGAAGGAAA GGGGGAAACC TCCCAGTTTA CGATATTGTC CAAATCACAC 
AGTGAGGTAG AAGATGTTCA GAAAGTGTGT GTACGTTTGC CCTCTTCCCC ACTGCTTGAA 
ACAGCTTTAC AAATGAAAGC GTATGCCCAC AGTGACAAGC TCAGTGCCTT CAACAATAAT 
CCATGTCTTG AGAAAGTATT ACCAGAGGCA ACCCCAGTGG TTTTATCAGA AACATATAGG 
GAGGTACAAG ATTGTTTTGG ATCTTTACCT TCATCTGATT TGCATGAAAA TGAGCCTCCA 
CTGGAAGAGA GATCCCTGGT TGATAAATCT AATAGCTCCA GAAATTATAC TTGCCTTGAG 
AAAGTAGTAC CAGAGGCAAC CTTATTGTTA AAAGAGTACT TTTCAGAATC CTGTAGTAAG 
GTACAAGATG ATGAGAAATG TTTTGGATCT TTACCTTCAT CTGCTTTGCA AAGAAAAAAT 
CCTCTAATTG AAAAAAACGG ACCGAGTGAC AAATCCAGTG CATCCAGCAA TAATGTTTGC 
CTTGAGGAAG GAGAACCAGA GGCAGTCCCA TTGTTGGAAG ACTGTAAAAG TCAAACCCTT 
GTTGTACCTT CCACATGCAG TCAATTGTCC ATAACAGATA AAATAGCAAA CAGTCTTGGA 
GTACATAATT CCTCAGATTC ATGTCAGCTC ATCAGTGAAA TTCATCATGA TCATCCAAGC 
GATGGAAATG ATCTCACTGT GTCCCATATT GCCTTCAATG TTGGTGGTGC TGACAAAATC 
AATAAAGTTA AAAGCCTGCA GGTAACTCCA CTTCCAGAGG TTATATCTAC AGAACTATGT 
AGGGGCAAGA GCAACAGTCA AAGCACTTGT GAACCTCCAT CCGTGCCAGT TCTGTCATGT 
AAGCTTTCTT CTGAGCAAAA AGATGAAGGT ACATTGATGG AATTGTCTAG CTCTTTACAT 
AGTGAAACAA CTGAGGAGGA AGAATGTGGA AATAAAATGG CTCTTGGAAG TTCAACATTG 
GATGGGGAAA TATCTGACTA TAAGAAACTA AACAGTCAAA TTAAGCCTTT GCCACAAGCA 
ATAGAAAGCT TGGTCATGGA CACGTGTGAT AGCAATCTGT GCAATAATAT TAATTTAAAA 
GATAGCAGCA AAATAATTTC AGAGGAAGCT ACAGTACCAC ATTGTTTTCA GCCAGAACAG 
CATGTTGTAT ACAGCCATCC TATGTCTGAC CCTGCAGTTA CTTGTGTGAC TCATGGCACT 
GGATCTGAAA TGAAAGCATT GGATACACCA AGGAAGGAGT CTAATCCAGT GTGTAAGGAA 
CTCCGTGAAA GAAATGTAAA TCCAATGAAA GAATTGTATA TATCTCTGAA CAATGCACCA 
ACTGATTTGT CTAACTCTTT GTGCAGTGAC ACTACTAATG TGGAAGAATC TAGGGATGCC 
ACAGCAAATG CAAGTTCTTT ATCCAACGGA GAAATATTGC ATTTATATCA CAAAACAAGC 
AGCCCAGCTC TTGTTGATCC AAAAGCAGAA GCAAATACAG TTTTAGGCTT GTGTGACAGT 
GTCCTCAGCA AAAACTCTAC AATGGGAAGT AGTGATAGCA TTAATCAGGA GAAAGCTATA 
GCAACAACTT TTGCACACCC AGGACAGAGT GTGTTGTTCA GCCATGGGGC ATCTACAATT 
ACAGTCTCCC CTGGTTTATT TCATAGTGCT GTACAGGCAT TAGATGTTTC TGATACACCA 
GAGTTGGGGT GCAGTCCTGT GTGTAAGGAA CCCACTGTTG TACAAGATAA AAGCGATGGA 
TCCTTTGATA CTTCTTTGGG CCATTTTGAA AGAGAAAAGC AAAATGAGAG CTTTGAAACA 
GACAGTTCTG TAATGCTTAC TGATGATCAC ATAAAGGATC CAAAGGATGT AGATGTTGTA 
TTGTCTTTGC CAGAGGGGAG CTTGGAAGAT TGCTGTCCCA AAAAAAAATC AAACTGCTCA 
GAGAACATGC ATGTAGCCTT AAAGGGTACC TCCACAAACC CTTCCCTTGA ACTCGGTTCC 
CACGATGCCC CGTGTGAAAA GAAGCTGCTT CAATGGAGAA ACACCCTCTT CCTCTGCTGG 
TTGGACTGCA TATTGGCAGC ACTGGTGCAT TCTGCTAGCC TGAATAGCAT TGTGGCACAG 
CGAAGGGTTG CAGAAAGCTC AATCATTCAC CTTCTTCTGG AGAAATACAA GGAGGCCAAT 
GCATTAATAA AAAGCACATC CAGACGAGGA CGTCCAAAGA ATTTATCTCA AGCTGAAAAC 
ATCCTGAATA AAGTGCGAAT GACCATCTTT GATGAAATAA AGCCCTTGTT AAAGTGTGAA 
TTGGGAGATA AAGAGAGCCC TGTGTTTGCT TTGCCGTTAC TGCTGCAGCT GGTTCCAGAG 
ATAGAATCCT GTTTTGTGCA CTCAGGTGTA TGGGAGTTTA CATGCAAGCT ATGTGGCTAC 
CAATACCAAA ATAAGCACCA ACGGACTGTG TCCACATTCA CTAAGATCTT ACCTGAATGG 
ACACCCCTAA ATGCTATCCA TAAAGCTCCT TGCAACAAAT GCCTGGGTAC AGATCAGATA 
CGTGCTTTTC GTATTAAAAA GATCCATGAC CTATTCATAC TTCACTTTGT GGAAGGCCTG 
CCAAGCAATG ATTTAGGCAT GTACTCGTTT CACTTTGAAG GCTGCCTGTA TGAAGTAAAG 
ACTGTCATCC AGTATAGTGA AGACCATTTC TCATCTTGGA TTGCTAATGA GGATGGCACA 
TGGCTGGAAT CTGATGACCT GAAAGGGTAT TTCTGCATAA AACAGAACTG TTTTGAGGTT 
CCTGCTTCAG AAATTCATAT TGTAATATGG GAAAAGAAGA TTACCAACAG CACTGGAGCA 
CACCATGCAA TGGAACCTGA AACAAAAAAC AAAGCACTTA ATTCTTTATC TACTTCACCT 
TCATCTTTAT CTCAGACCCT GCACCTGTCT CCTTCAGCTG CTCCGAATGC TGCATCTTCA 
CCTCTAAGAG ATGCCCCAAA TGCTTCCAGC GTACTCTCTG GAATGGAGGG TTTTCATGAT 
GACGACATCA TAACGCTCAC TCTCATTGAA ATTCCAATTG ATGCATCGGG TAATGTGATT 
GAGAACAACT CTGAGGCAGC CCAGCAGCCA AGTCTCATTG CTGAAACACA TTCATCCCCT 
GTAACCCTCA AAGCAGTTGC TGATGGGCAA TGTGATCAAA ATCTATGTTC TGGTACAATG 
AAGGAAAGCT CTTTCGCTGG TGGTTCTCTT CCAGTTCCTA ACATTTCCAG CCCTCCTGCC 
CCTAACATTT CCAGCCCTCC TGCCCCTAAC ATTTCCAGCC CTCCTGCCCC TAACATTTCC 
AGCCCTCCTG CCTCTAACAT TTCCAGCCCT CCTGCCCCTC ACATTTCCAG CCCTCCTGCC 
CCTCACATTT CCAGCCCTCC TGCCCCTCAC ATTTCCAGCC CTCCTGCCCC TCACATTTCC 
AGCCCTCCTG CCCCTCACAT TTCCAGCCCT CCTGCCCCTC ACATTTCCAG CCCTCCTGCC 
CCTAACAGCG CCAGTCCCCC TGCCCCTAAC AGCGCCAGTA CTCCCCTCCC TAACAGTTCC 
AGTTCAAAAA AAGCCTTTGT TGGAAGTTGG ATGAAGAGTC TACTAAATAA AAACCCTTTG 
TTCCTGACAT CTAATGTGCC CTGTGCAAAT AAGAAAACTG TGGCAAAAAC TCCAATCCGA 
TTTCCCCTGC TGAAGACAAC AGATTTGCAG GCATCTACTA AACAGGCCCA GAGCTTTGAT 
GGTTTTAAAG CTAAACGTGT CGGTAATTCA TTCAGCTCGG ACAATTGCCT TGGTGTAGGT 
CCCACGAAGG ATAATTCCAC TGCACCCAAA CCTGCCATCT CTAGCCAAAC GTTCCAGCCA 
CTGGTTTCAA ATCGACTTAA AAATGGGCGG TTAGGACTCG GTAATACCAA AGACTTGGCA 
AAAAATGTTT CTCTGTCTAA GGAGGATAAG GTGCTTAGGC TTAGACTGAA AATGCTGAAA 
AAGTTGAAGG CTAAGAAACA TGAATTGGCC GCCATAGATA TGCTTGGAAA GTCACAGGCT 
GGTAGTGATC TGGAGCGTAG CAGCACCAGC AGGCGTGAAC AATTACGCGG GTTCTTGCAG 
GAACTCCAAG AGCAAATCGA TAATGCCGAC ACTGAATCAG TGTGCACTAT GAGCTCTTGC 
ACGTCGATAT GCAGCAGCCC AGGAGATGCG GAATTCTTTG CGGAATTGTT CTCCCCACCC 
CCTAGCAACG CCCCGCAGGA GAGCATGGAT GACAGTAATT TTTTAGAGAT GTATGTAGAT 
GGTTACAACC CCTCCTGTGG CAGTTATGGA ACCAGTAACC CAAGTGGCAA AGAGGTCTCA 
CAGGACAGTG CTAATATCGG CCAGTCCAAT TTACAGCGTG CTTCCGACAG CCACAATAAC 
ACTAACGTGG GGGACGTCTT CCATGATATG CTGTCAACCT CCACAATGCA CATGCTGAAC 
GAGGATTATT TCTCACCTTT TGATGATATG TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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uspl1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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電話

FAX

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