lig1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following lig1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lig1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa61549 XM_002941883.5
Latest version!
Xenopus tropicalis ligase I, DNA, ATP-dependent (lig1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa13271 XM_002941883.4
Latest version!
Xenopus tropicalis ligase I, DNA, ATP-dependent (lig1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa61549
    Clone ID Related Accession (Same CDS sequence) XM_002941883.5
    Accession Version XM_002941883.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3123bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA ligase 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002941883.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCAACGAA CAATCAAGTC CTTTTTTCAG CCCAAACTTG GGGCTGAAGT AAAGAAAAAG 
    GAAGAGAATG TGAAGCATGA TGATACTAAA AAAGAAAAGG TCGAAGAGCC AGAGAAAAGT
    GTTAATACTG AACGTCCTCT AAAAGAGAGA AATGGCAGTG CTTTGTGTGA TGATGCCGAA
    TCACCCGTCA AAAAAGTTTC AAAGAAGTCT GCACGTGTGT TGGAAAGTGA TAGTGAAGAG
    GAAGAGGAAA ACTCAAAGGT ACAAGCGACT CCTGAAAAAA TGAACAATGA TAGTGTACCA
    AAGAATGATG CAGTTGAAGA AACTCCTCAG AGAGCACATA AGGAAACAAA AGTTGAAGGA
    ACTTCAGAGA TGAACACTTC ACAAGATTTT ACTTCTCCTT GCAGCAGCAA ATCTATTGAC
    AGCCCTTTAT GTTCAGATTC TCCTGGCATA TCTCCATCTG GTATCCCCAA AAGAAAGACA
    GCCCGGAAAC AACTACCAAA ACGTAAGCTG GAAAATTCTC CCAGTGAATC AAATCCACCC
    ACAGAGGATG TAAAAGTTCA AGGAGCAGTG AAGCGCCAAA GGAAAGAGGC AGAGGGAAAT
    GTTCAACAAG AAGAACCCAT GGAGACAGGT CACAATATCT CCATGGAAGA GGAATGCTCC
    ATAAAGAAAG AGGCAAATGC TGAAGTGGTT CAAGAAGAAA AATCCAAAGT GGATGATCAG
    AATGTGGAAC TTTTAGACCA AAAGGATGAT GTTTCAGGAA GAGAACAATC TGAGCCTAAA
    GATAAAGAAC TGCAGCCTAA ATCTACCAGT CCTAATTCCA AGCCAAAAAC GCCAAAGGCA
    AAGTCAGGAA AGAAACACAG TCCATTGCAA AAGAAAGTGA AATTTAGCGA TAAGGTATCT
    TCAAAAGATG ACAGTGAAGG AAAAAGTAGC GAGGAAAAAA GTGATGAGGA GCCCGAGAAG
    AAAGCAGATC CTGCTAAACC CGTGAAACAA ATAAGCAGCT TCTTTGCCCC AAAGAAACCT
    GCAATAAAGA CAGAAAAAAA AGAGGAAATT ATGAATGAAA AGAATGCAAG TGAGACCTCT
    TTGGAAGCAT CCCCCAAACC TAAAAAGACA GTTAGCAGTT TCTTTGGAGC AATAAAGCCA
    GAACCATCAG AAGACCAGGC AGTGTATAAC CCATCCAAGA GTAGTTATCA CCCCATAAAT
    GATGCTTGCT GGTCTAATGG ACAGAAGGTG CCATACCTTG CTGTTGCACG AACCTTTGAG
    AGGATAGAAG AGGAATCTGC ACGACTTAAG AACATAGAGA CTCTCAGTAA TTTTCTGCGC
    TCCGTCATTG CTCTCACTCC TGGGGACCTC TTGCCTTGCA TCTATCTTTG TCTGAACCGC
    TTGGGACCTG CCTATGAAGG ACTGGAGCTC GGCATAGGAG AAACTATATT GATGAAAGCA
    GTTGCCCAGG CTACAGGACG CCAGCTTGAG AAAATAAAGT CTGAGGCACA AGAGAAAGGA
    GACCTTGGCC TGGTTGCTGA AAGTAGTCGT AGCAACCAGC GCACTATGTT TACACCCCCA
    AAGCTCATGG CATCAGGTGT TTTTAGCAAG CTGAAAGATA TAGCCAGGAT GACTGGCAAT
    GCTTCTATGA ATAAAAAAAT TGATATTATT AAAGGACTGT TTGTAGCCTG CAGGCATTCT
    GAGGCGCGTT ATATTGCCAG ATCTCTTGGA GGAAAACTTC GGATTGGTCT GGCAGAGCAG
    TCAGTGTTAT CAGCAATTGC TCAGGCAGTG TGCCTGACTC CTCCTGGGCA CGATGCCCCT
    CCTACTGTGA TGGATGCTGG AAAGGGAATG AGTACAGATG CCAGAAAATC CTGGATAGAA
    GACAAAGCAA TGATCTTGAA ACAAACTTTT TGTGAGTTGC CGAACTATGA TGCCATAATT
    CCTATCCTTT TGGAACATGG TATTGATGAC CTCCCAAAAC ACTGCCGACT TACTCCAGGG
    ATCCCTTTGA AACCAATGTT GGCGCATCCA ACCAAGGGTA TTGGGGAAGT GCTAAAGCGA
    TTTGATGAAG CTGCCTTTAC GTGTGAATAC AAGTATGATG GGGAGCGTGC ACAGATACAT
    ATTCTGGAAA ATGGGGAGGT ACATATATAT AGCAGAAACC AAGAAAACAA CACCACTAAA
    TACCCTGACA TCATCAGCAG AATTCCCAAG ATTAAAAAAG AAAGCGTCAA ATCCTGTATC
    CTCGATACAG AGGCTGTAGC CTGGGACCCA GAAAAGAAGC AAATTCAGCC GTTTCAAGTA
    CTGACTACTA GAAAGAGAAA GGATGTGGAT GCATCAGAGA TTAAAGTGCA AGTTTGTGTG
    TATGCCTTTG ACATGCTTTA CCTTAATGGA GAGTCCCTAG TGAAAGAGCC ATTTGCTAAG
    CGACGACAGC TCTTGAGGGA TTCGTTCCTG GAGACAGAAG GGGAGTTTAT GTTTGCTACC
    TATATGGATA CATCAAATAC AGATGAAATT TCTGAGTTTC TTGATCAGTC TATTAAAGAT
    TCATGTGAAG GACTTATGGT GAAGACACTG GAGCAAGATG CTACATATGA AATTGCAAAG
    CGATCTCACA ATTGGTTAAA ACTAAAGAAG GATTACCTGG AAGGTGTGGG TGATACACTG
    GATCTGGTTG TTATTGGAGC TTACCTAGGA AAAGGAAAAA GAACGGGTAT TTATGGAGGC
    TTCCTGCTTG CGTCATATGA TGAGGAAAGT GAAGAATATC AGACCATCTG CAAGATTGGA
    ACAGGTTTTA CTGATGAAGA CTTAGAAAAG CATTACAACT ACCTGAAGGA TCATGTTATT
    GACAGCCCAC GGAGCTACTA CCGGTGGGAC AGCGCCACAG AGCCGGATCA CTGGTTTGAT
    CCTGTACAAG TCTGGGAAGT GAAATGTGCT GATCTGTCCA TTTCTCCAGT ACATAAGGCA
    GCTCTTGGAT TGGTGGAGGA CGAGAAGGGA ATTTCCCTGC GATTTCCTCG CTTCCTTCGG
    ATCAGAGACG ATAAGAAACC AGAGGAGGCC ACAAACAGTT TTCAGGTAGC AGATCTTTAT
    AGAAAGCAGC AACAAATCCA AAACACAACT ACAACAGAGA AGGGAGAAGA GGATTTCTAC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002941929.1
    CDS156..3278
    Translation

    Target ORF information:

    RefSeq Version XM_002941883.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ligase I, DNA, ATP-dependent (lig1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002941883.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCAACGAA CAATCAAGTC CTTTTTTCAG CCCAAACTTG GGGCTGAAGT AAAGAAAAAG 
    GAAGAGAATG TGAAGCATGA TGATACTAAA AAAGAAAAGG TCGAAGAGCC AGAGAAAAGT
    GTTAATACTG AACGTCCTCT AAAAGAGAGA AATGGCAGTG CTTTGTGTGA TGATGCCGAA
    TCACCCGTCA AAAAAGTTTC AAAGAAGTCT GCACGTGTGT TGGAAAGTGA TAGTGAAGAG
    GAAGAGGAAA ACTCAAAGGT ACAAGCGACT CCTGAAAAAA TGAACAATGA TAGTGTACCA
    AAGAATGATG CAGTTGAAGA AACTCCTCAG AGAGCACATA AGGAAACAAA AGTTGAAGGA
    ACTTCAGAGA TGAACACTTC ACAAGATTTT ACTTCTCCTT GCAGCAGCAA ATCTATTGAC
    AGCCCTTTAT GTTCAGATTC TCCTGGCATA TCTCCATCTG GTATCCCCAA AAGAAAGACA
    GCCCGGAAAC AACTACCAAA ACGTAAGCTG GAAAATTCTC CCAGTGAATC AAATCCACCC
    ACAGAGGATG TAAAAGTTCA AGGAGCAGTG AAGCGCCAAA GGAAAGAGGC AGAGGGAAAT
    GTTCAACAAG AAGAACCCAT GGAGACAGGT CACAATATCT CCATGGAAGA GGAATGCTCC
    ATAAAGAAAG AGGCAAATGC TGAAGTGGTT CAAGAAGAAA AATCCAAAGT GGATGATCAG
    AATGTGGAAC TTTTAGACCA AAAGGATGAT GTTTCAGGAA GAGAACAATC TGAGCCTAAA
    GATAAAGAAC TGCAGCCTAA ATCTACCAGT CCTAATTCCA AGCCAAAAAC GCCAAAGGCA
    AAGTCAGGAA AGAAACACAG TCCATTGCAA AAGAAAGTGA AATTTAGCGA TAAGGTATCT
    TCAAAAGATG ACAGTGAAGG AAAAAGTAGC GAGGAAAAAA GTGATGAGGA GCCCGAGAAG
    AAAGCAGATC CTGCTAAACC CGTGAAACAA ATAAGCAGCT TCTTTGCCCC AAAGAAACCT
    GCAATAAAGA CAGAAAAAAA AGAGGAAATT ATGAATGAAA AGAATGCAAG TGAGACCTCT
    TTGGAAGCAT CCCCCAAACC TAAAAAGACA GTTAGCAGTT TCTTTGGAGC AATAAAGCCA
    GAACCATCAG AAGACCAGGC AGTGTATAAC CCATCCAAGA GTAGTTATCA CCCCATAAAT
    GATGCTTGCT GGTCTAATGG ACAGAAGGTG CCATACCTTG CTGTTGCACG AACCTTTGAG
    AGGATAGAAG AGGAATCTGC ACGACTTAAG AACATAGAGA CTCTCAGTAA TTTTCTGCGC
    TCCGTCATTG CTCTCACTCC TGGGGACCTC TTGCCTTGCA TCTATCTTTG TCTGAACCGC
    TTGGGACCTG CCTATGAAGG ACTGGAGCTC GGCATAGGAG AAACTATATT GATGAAAGCA
    GTTGCCCAGG CTACAGGACG CCAGCTTGAG AAAATAAAGT CTGAGGCACA AGAGAAAGGA
    GACCTTGGCC TGGTTGCTGA AAGTAGTCGT AGCAACCAGC GCACTATGTT TACACCCCCA
    AAGCTCATGG CATCAGGTGT TTTTAGCAAG CTGAAAGATA TAGCCAGGAT GACTGGCAAT
    GCTTCTATGA ATAAAAAAAT TGATATTATT AAAGGACTGT TTGTAGCCTG CAGGCATTCT
    GAGGCGCGTT ATATTGCCAG ATCTCTTGGA GGAAAACTTC GGATTGGTCT GGCAGAGCAG
    TCAGTGTTAT CAGCAATTGC TCAGGCAGTG TGCCTGACTC CTCCTGGGCA CGATGCCCCT
    CCTACTGTGA TGGATGCTGG AAAGGGAATG AGTACAGATG CCAGAAAATC CTGGATAGAA
    GACAAAGCAA TGATCTTGAA ACAAACTTTT TGTGAGTTGC CGAACTATGA TGCCATAATT
    CCTATCCTTT TGGAACATGG TATTGATGAC CTCCCAAAAC ACTGCCGACT TACTCCAGGG
    ATCCCTTTGA AACCAATGTT GGCGCATCCA ACCAAGGGTA TTGGGGAAGT GCTAAAGCGA
    TTTGATGAAG CTGCCTTTAC GTGTGAATAC AAGTATGATG GGGAGCGTGC ACAGATACAT
    ATTCTGGAAA ATGGGGAGGT ACATATATAT AGCAGAAACC AAGAAAACAA CACCACTAAA
    TACCCTGACA TCATCAGCAG AATTCCCAAG ATTAAAAAAG AAAGCGTCAA ATCCTGTATC
    CTCGATACAG AGGCTGTAGC CTGGGACCCA GAAAAGAAGC AAATTCAGCC GTTTCAAGTA
    CTGACTACTA GAAAGAGAAA GGATGTGGAT GCATCAGAGA TTAAAGTGCA AGTTTGTGTG
    TATGCCTTTG ACATGCTTTA CCTTAATGGA GAGTCCCTAG TGAAAGAGCC ATTTGCTAAG
    CGACGACAGC TCTTGAGGGA TTCGTTCCTG GAGACAGAAG GGGAGTTTAT GTTTGCTACC
    TATATGGATA CATCAAATAC AGATGAAATT TCTGAGTTTC TTGATCAGTC TATTAAAGAT
    TCATGTGAAG GACTTATGGT GAAGACACTG GAGCAAGATG CTACATATGA AATTGCAAAG
    CGATCTCACA ATTGGTTAAA ACTAAAGAAG GATTACCTGG AAGGTGTGGG TGATACACTG
    GATCTGGTTG TTATTGGAGC TTACCTAGGA AAAGGAAAAA GAACGGGTAT TTATGGAGGC
    TTCCTGCTTG CGTCATATGA TGAGGAAAGT GAAGAATATC AGACCATCTG CAAGATTGGA
    ACAGGTTTTA CTGATGAAGA CTTAGAAAAG CATTACAACT ACCTGAAGGA TCATGTTATT
    GACAGCCCAC GGAGCTACTA CCGGTGGGAC AGCGCCACAG AGCCGGATCA CTGGTTTGAT
    CCTGTACAAG TCTGGGAAGT GAAATGTGCT GATCTGTCCA TTTCTCCAGT ACATAAGGCA
    GCTCTTGGAT TGGTGGAGGA CGAGAAGGGA ATTTCCCTGC GATTTCCTCG CTTCCTTCGG
    ATCAGAGACG ATAAGAAACC AGAGGAGGCC ACAAACAGTT TTCAGGTAGC AGATCTTTAT
    AGAAAGCAGC AACAAATCCA AAACACAACT ACAACAGAGA AGGGAGAAGA GGATTTCTAC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa13271
    Clone ID Related Accession (Same CDS sequence) XM_002941883.4
    Accession Version XM_002941883.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3123bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA ligase 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002941883.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCAACGAA CAATCAAGTC CTTTTTTCAG CCCAAACTTG GGGCTGAAGT AAAGAAAAAG 
    GAAGAGAATG TGAAGCATGA TGATACTAAA AAAGAAAAGG TCGAAGAGCC AGAGAAAAGT
    GTTAATACTG AACGTCCTCT AAAAGAGAGA AATGGCAGTG CTTTGTGTGA TGATGCCGAA
    TCACCCGTCA AAAAAGTTTC AAAGAAGTCT GCACGTGTGT TGGAAAGTGA TAGTGAAGAG
    GAAGAGGAAA ACTCAAAGGT ACAAGCGACT CCTGAAAAAA TGAACAATGA TAGTGTACCA
    AAGAATGATG CAGTTGAAGA AACTCCTCAG AGAGCACATA AGGAAACAAA AGTTGAAGGA
    ACTTCAGAGA TGAACACTTC ACAAGATTTT ACTTCTCCTT GCAGCAGCAA ATCTATTGAC
    AGCCCTTTAT GTTCAGATTC TCCTGGCATA TCTCCATCTG GTATCCCCAA AAGAAAGACA
    GCCCGGAAAC AACTACCAAA ACGTAAGCTG GAAAATTCTC CCAGTGAATC AAATCCACCC
    ACAGAGGATG TAAAAGTTCA AGGAGCAGTG AAGCGCCAAA GGAAAGAGGC AGAGGGAAAT
    GTTCAACAAG AAGAACCCAT GGAGACAGGT CACAATATCT CCATGGAAGA GGAATGCTCC
    ATAAAGAAAG AGGCAAATGC TGAAGTGGTT CAAGAAGAAA AATCCAAAGT GGATGATCAG
    AATGTGGAAC TTTTAGACCA AAAGGATGAT GTTTCAGGAA GAGAACAATC TGAGCCTAAA
    GATAAAGAAC TGCAGCCTAA ATCTACCAGT CCTAATTCCA AGCCAAAAAC GCCAAAGGCA
    AAGTCAGGAA AGAAACACAG TCCATTGCAA AAGAAAGTGA AATTTAGCGA TAAGGTATCT
    TCAAAAGATG ACAGTGAAGG AAAAAGTAGC GAGGAAAAAA GTGATGAGGA GCCCGAGAAG
    AAAGCAGATC CTGCTAAACC CGTGAAACAA ATAAGCAGCT TCTTTGCCCC AAAGAAACCT
    GCAATAAAGA CAGAAAAAAA AGAGGAAATT ATGAATGAAA AGAATGCAAG TGAGACCTCT
    TTGGAAGCAT CCCCCAAACC TAAAAAGACA GTTAGCAGTT TCTTTGGAGC AATAAAGCCA
    GAACCATCAG AAGACCAGGC AGTGTATAAC CCATCCAAGA GTAGTTATCA CCCCATAAAT
    GATGCTTGCT GGTCTAATGG ACAGAAGGTG CCATACCTTG CTGTTGCACG AACCTTTGAG
    AGGATAGAAG AGGAATCTGC ACGACTTAAG AACATAGAGA CTCTCAGTAA TTTTCTGCGC
    TCCGTCATTG CTCTCACTCC TGGGGACCTC TTGCCTTGCA TCTATCTTTG TCTGAACCGC
    TTGGGACCTG CCTATGAAGG ACTGGAGCTC GGCATAGGAG AAACTATATT GATGAAAGCA
    GTTGCCCAGG CTACAGGACG CCAGCTTGAG AAAATAAAGT CTGAGGCACA AGAGAAAGGA
    GACCTTGGCC TGGTTGCTGA AAGTAGTCGT AGCAACCAGC GCACTATGTT TACACCCCCA
    AAGCTCATGG CATCAGGTGT TTTTAGCAAG CTGAAAGATA TAGCCAGGAT GACTGGCAAT
    GCTTCTATGA ATAAAAAAAT TGATATTATT AAAGGACTGT TTGTAGCCTG CAGGCATTCT
    GAGGCACGTT ATATTGCCAG ATCTCTTGGA GGAAAACTTC GGATTGGTCT GGCAGAGCAG
    TCAGTGTTAT CAGCAATTGC TCAGGCAGTG TGCCTGACTC CTCCTGGGCA CGATGCCCCT
    CCTACTGTGA TGGATGCTGG AAAGGGAATG AGTACAGATG CCAGAAAATC CTGGATAGAA
    GACAAAGCAA TGATCTTGAA ACAAACTTTT TGTGAGTTGC CGAACTATGA TGCCATAATT
    CCTATCCTTT TGGAACATGG TATTGATGAC CTCCCAAAAC ACTGCCGACT TACTCCAGGG
    ATCCCTTTGA AACCAATGTT GGCGCATCCA ACCAAGGGTA TTGGGGAAGT GCTAAAGCGA
    TTTGATGAAG CTGCCTTTAC GTGTGAATAC AAGTATGATG GGGAGCGTGC ACAGATACAT
    ATTCTGGAAA ATGGGGAGGT ACATATATAT AGCAGAAACC AAGAAAACAA CACCACTAAA
    TACCCTGACA TCATCAGCAG AATTCCCAAG ATTAAAAAAG AAAGCGTCAA ATCCTGTATC
    CTCGATACAG AGGCTGTAGC CTGGGACCCA GAAAAGAAGC AAATTCAGCC GTTTCAAGTA
    CTGACTACTA GAAAGAGAAA GGATGTGGAT GCATCAGAGA TTAAAGTGCA AGTTTGTGTG
    TATGCCTTTG ACATGCTTTA CCTTAATGGA GAGTCCCTAG TGAAAGAGCC ATTTGCTAAG
    CGACGACAGC TCTTGAGGGA TTCGTTCCTG GAGACAGAAG GGGAGTTTAT GTTTGCTACC
    TATATGGATA CATCAAATAC AGATGAAATT TCTGAGTTTC TTGATCAGTC TATTAAAGAT
    TCATGTGAAG GACTTATGGT GAAGACACTG GAGCAAGATG CTACATATGA AATTGCAAAG
    CGATCTCACA ATTGGTTAAA ACTAAAGAAG GATTACCTGG AAGGTGTGGG TGATACACTG
    GATCTGGTTG TTATTGGAGC TTACCTAGGA AAAGGAAAAA GAACGGGTAT TTATGGAGGC
    TTCCTGCTTG CGTCATATGA TGAGGAAAGT GAAGAATATC AGACCATCTG CAAGATTGGA
    ACAGGTTTTA CTGATGAAGA CTTAGAAAAG CATTACAACT ACCTGAAGGA TCATGTTATT
    GACAGCCCAC GGAGCTACTA CCGGTGGGAC AGCGCCACAG AGCCGGATCA CTGGTTTGAT
    CCTGTACAAG TCTGGGAAGT GAAATGTGCT GATCTGTCCA TTTCTCCAGT ACATAAGGCA
    GCTCTTGGAT TGGTGGAGGA TGAGAAGGGA ATTTCCCTGC GATTTCCTCG CTTCCTTCGG
    ATCAGAGACG ATAAGAAACC AGAGGAGGCC ACAAACAGTT TTCAGGTAGC AGATCTTTAT
    AGAAAGCAGC AACAAATCCA AAACACAACT ACAACAGAGA AGGGAGAAGA GGATTTCTAC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002941929.1
    CDS634..3756
    Translation

    Target ORF information:

    RefSeq Version XM_002941883.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ligase I, DNA, ATP-dependent (lig1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002941883.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCAACGAA CAATCAAGTC CTTTTTTCAG CCCAAACTTG GGGCTGAAGT AAAGAAAAAG 
    GAAGAGAATG TGAAGCATGA TGATACTAAA AAAGAAAAGG TCGAAGAGCC AGAGAAAAGT
    GTTAATACTG AACGTCCTCT AAAAGAGAGA AATGGCAGTG CTTTGTGTGA TGATGCCGAA
    TCACCCGTCA AAAAAGTTTC AAAGAAGTCT GCACGTGTGT TGGAAAGTGA TAGTGAAGAG
    GAAGAGGAAA ACTCAAAGGT ACAAGCGACT CCTGAAAAAA TGAACAATGA TAGTGTACCA
    AAGAATGATG CAGTTGAAGA AACTCCTCAG AGAGCACATA AGGAAACAAA AGTTGAAGGA
    ACTTCAGAGA TGAACACTTC ACAAGATTTT ACTTCTCCTT GCAGCAGCAA ATCTATTGAC
    AGCCCTTTAT GTTCAGATTC TCCTGGCATA TCTCCATCTG GTATCCCCAA AAGAAAGACA
    GCCCGGAAAC AACTACCAAA ACGTAAGCTG GAAAATTCTC CCAGTGAATC AAATCCACCC
    ACAGAGGATG TAAAAGTTCA AGGAGCAGTG AAGCGCCAAA GGAAAGAGGC AGAGGGAAAT
    GTTCAACAAG AAGAACCCAT GGAGACAGGT CACAATATCT CCATGGAAGA GGAATGCTCC
    ATAAAGAAAG AGGCAAATGC TGAAGTGGTT CAAGAAGAAA AATCCAAAGT GGATGATCAG
    AATGTGGAAC TTTTAGACCA AAAGGATGAT GTTTCAGGAA GAGAACAATC TGAGCCTAAA
    GATAAAGAAC TGCAGCCTAA ATCTACCAGT CCTAATTCCA AGCCAAAAAC GCCAAAGGCA
    AAGTCAGGAA AGAAACACAG TCCATTGCAA AAGAAAGTGA AATTTAGCGA TAAGGTATCT
    TCAAAAGATG ACAGTGAAGG AAAAAGTAGC GAGGAAAAAA GTGATGAGGA GCCCGAGAAG
    AAAGCAGATC CTGCTAAACC CGTGAAACAA ATAAGCAGCT TCTTTGCCCC AAAGAAACCT
    GCAATAAAGA CAGAAAAAAA AGAGGAAATT ATGAATGAAA AGAATGCAAG TGAGACCTCT
    TTGGAAGCAT CCCCCAAACC TAAAAAGACA GTTAGCAGTT TCTTTGGAGC AATAAAGCCA
    GAACCATCAG AAGACCAGGC AGTGTATAAC CCATCCAAGA GTAGTTATCA CCCCATAAAT
    GATGCTTGCT GGTCTAATGG ACAGAAGGTG CCATACCTTG CTGTTGCACG AACCTTTGAG
    AGGATAGAAG AGGAATCTGC ACGACTTAAG AACATAGAGA CTCTCAGTAA TTTTCTGCGC
    TCCGTCATTG CTCTCACTCC TGGGGACCTC TTGCCTTGCA TCTATCTTTG TCTGAACCGC
    TTGGGACCTG CCTATGAAGG ACTGGAGCTC GGCATAGGAG AAACTATATT GATGAAAGCA
    GTTGCCCAGG CTACAGGACG CCAGCTTGAG AAAATAAAGT CTGAGGCACA AGAGAAAGGA
    GACCTTGGCC TGGTTGCTGA AAGTAGTCGT AGCAACCAGC GCACTATGTT TACACCCCCA
    AAGCTCATGG CATCAGGTGT TTTTAGCAAG CTGAAAGATA TAGCCAGGAT GACTGGCAAT
    GCTTCTATGA ATAAAAAAAT TGATATTATT AAAGGACTGT TTGTAGCCTG CAGGCATTCT
    GAGGCACGTT ATATTGCCAG ATCTCTTGGA GGAAAACTTC GGATTGGTCT GGCAGAGCAG
    TCAGTGTTAT CAGCAATTGC TCAGGCAGTG TGCCTGACTC CTCCTGGGCA CGATGCCCCT
    CCTACTGTGA TGGATGCTGG AAAGGGAATG AGTACAGATG CCAGAAAATC CTGGATAGAA
    GACAAAGCAA TGATCTTGAA ACAAACTTTT TGTGAGTTGC CGAACTATGA TGCCATAATT
    CCTATCCTTT TGGAACATGG TATTGATGAC CTCCCAAAAC ACTGCCGACT TACTCCAGGG
    ATCCCTTTGA AACCAATGTT GGCGCATCCA ACCAAGGGTA TTGGGGAAGT GCTAAAGCGA
    TTTGATGAAG CTGCCTTTAC GTGTGAATAC AAGTATGATG GGGAGCGTGC ACAGATACAT
    ATTCTGGAAA ATGGGGAGGT ACATATATAT AGCAGAAACC AAGAAAACAA CACCACTAAA
    TACCCTGACA TCATCAGCAG AATTCCCAAG ATTAAAAAAG AAAGCGTCAA ATCCTGTATC
    CTCGATACAG AGGCTGTAGC CTGGGACCCA GAAAAGAAGC AAATTCAGCC GTTTCAAGTA
    CTGACTACTA GAAAGAGAAA GGATGTGGAT GCATCAGAGA TTAAAGTGCA AGTTTGTGTG
    TATGCCTTTG ACATGCTTTA CCTTAATGGA GAGTCCCTAG TGAAAGAGCC ATTTGCTAAG
    CGACGACAGC TCTTGAGGGA TTCGTTCCTG GAGACAGAAG GGGAGTTTAT GTTTGCTACC
    TATATGGATA CATCAAATAC AGATGAAATT TCTGAGTTTC TTGATCAGTC TATTAAAGAT
    TCATGTGAAG GACTTATGGT GAAGACACTG GAGCAAGATG CTACATATGA AATTGCAAAG
    CGATCTCACA ATTGGTTAAA ACTAAAGAAG GATTACCTGG AAGGTGTGGG TGATACACTG
    GATCTGGTTG TTATTGGAGC TTACCTAGGA AAAGGAAAAA GAACGGGTAT TTATGGAGGC
    TTCCTGCTTG CGTCATATGA TGAGGAAAGT GAAGAATATC AGACCATCTG CAAGATTGGA
    ACAGGTTTTA CTGATGAAGA CTTAGAAAAG CATTACAACT ACCTGAAGGA TCATGTTATT
    GACAGCCCAC GGAGCTACTA CCGGTGGGAC AGCGCCACAG AGCCGGATCA CTGGTTTGAT
    CCTGTACAAG TCTGGGAAGT GAAATGTGCT GATCTGTCCA TTTCTCCAGT ACATAAGGCA
    GCTCTTGGAT TGGTGGAGGA TGAGAAGGGA ATTTCCCTGC GATTTCCTCG CTTCCTTCGG
    ATCAGAGACG ATAAGAAACC AGAGGAGGCC ACAAACAGTT TTCAGGTAGC AGATCTTTAT
    AGAAAGCAGC AACAAATCCA AAACACAACT ACAACAGAGA AGGGAGAAGA GGATTTCTAC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.