eif2ak4 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following eif2ak4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the eif2ak4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa32794 XM_012968127.3
Latest version!
Xenopus tropicalis eukaryotic translation initiation factor 2 alpha kinase 4 (eif2ak4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa32795 XM_012968128.3
Latest version!
Xenopus tropicalis eukaryotic translation initiation factor 2 alpha kinase 4 (eif2ak4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa45985 XM_018096178.1
Latest version!
Xenopus tropicalis eukaryotic translation initiation factor 2 alpha kinase 4 (eif2ak4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OXa20310 NM_001256926.1
Latest version!
Xenopus tropicalis eukaryotic translation initiation factor 2 alpha kinase 4 (eif2ak4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
View Old Accession Versions >>
OXa32794 XM_012968127.2 Xenopus tropicalis eukaryotic translation initiation factor 2 alpha kinase 4 (eif2ak4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OXa32795 XM_012968128.2 Xenopus tropicalis eukaryotic translation initiation factor 2 alpha kinase 4 (eif2ak4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa32794
    Clone ID Related Accession (Same CDS sequence) XM_012968127.2 , XM_012968127.3
    Accession Version XM_012968127.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5025bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product eIF-2-alpha kinase GCN2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012968127.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGCAGGCG GCCGGGGAAA TGCACTTCTT CCTATTGAGC TCAGCCCGCA AGGGGGTCTG 
    CGCCCTACTC CGCCTGCCTG TGACCCCCCT GAAACTTATT CCCACCGGCA GGAGAATGAG
    CTGGAGGTCC TGGAATCCAT CTATAGTGGG GATTTCGCGG ATCTGCGCAA GAACTCTGCC
    TGGAAGGTAC AGCAGCCTCC AGAAGTCATC ATTGCCTTAC GTCCTCAGGG AAATGAGGTT
    CATGTCAAGG TGGATTTATG GGTAAAATGC CCTCAGAACT ATCCTGATGT TCCTCCTGAA
    ATAAAGCTGA AGAACATCCA GGGGTTGTCC AATGAAAATG TGAACTCTCT GACATCAAGC
    TTGGAAGATT TAGCAAAACA GCTTTGTGGA GAGGTAATGA TATACCAGCT TGCGGATCAT
    GTGCAGTGTT TTCTTACAGA GTATAACCAT CCACCTCCTA AATCCTTTCA TGAGGAGATG
    TTAAAAAACC TTCAGAAGCA GCAGGAGAAG CTTGCTCTTG AAGAAGAGAG ACGAATGCAA
    GAGATAAAGA TGAAAGAAGA GCAGACGAGA CGGGAAATCC TTAATGAAAT CCAGAAGCGA
    GAAGAAGAGA AAAAGGAAGA AAAAAGAAGG AAGGAAGTAG CAAAACAGGA ACGGATTGAA
    AACGCTAATC TAATGTGCCA AGAGAATGTA AATAAGATTA ATAATACAGG CAAGACAACA
    ACAGCTGCCT CGGATGGGTC ACTTCCTGAT CATGGAGGAA CCAACAGAAA TCGGCCACAT
    TCAGCAGGAC GCTCAAAGCG GGACCGACAG TATTCGGTGT GCCTCACTGA TGACTCAACC
    CCATTTGAAG TGTTAACCTT TGATATTGGA TGCAGTGGGC AAATTGTTGT TCACAGAGGA
    AAGTGCCTGG GGAAAGATGA GCTGCTGGGT AAATCTGTTT ACAATGGCTT GGAAACTGCT
    TCAGGACATT TTGTTGTAAT CTATGAATGG GTTCTCCAGT GGCAGAAAAA GATGGGCAAG
    TTTCTTACTA ATCAAGAAAA AGAAAAGATT GAAAAGTGTA AAAAACAGAT TCAGGGAACA
    GAAACCGAGT TCAGTTCTCT TATCAAACTG AGCCATCCAA ACATTGTGCA CTACCTCGCT
    ATGCGAATAA AAGAGAAAGA AGATTCCATT TCTGTCGGGA TCATAGCTGA ACATGTTAAT
    GGTACAAAGC TTTCCAGTTA CCTTGAGCAG GGTGTACCTG TTCCACTGGA TCAACTACGG
    CACTATACTG CCCAGCTGCT ATCGGCACTG GACTATTTGC ATTCTAATTC TGTGGTACAT
    AAAGTCATGT GCGCCTCCAG TGTTTTGGTG ACAGCTGACG GGAAAGTAAA ACTGACAGAC
    TATAGCCTTT CCAAACGCCT TGCTGACATC TGCAAAGAGG ACGTGTTTGA GCAAACAAAA
    GTTCGATTCA GTGAGGATGC TCTGCCTAGC AAGAGTGGCA AGAAAGGAGA TATATGGAAG
    CTCGGTCTCT TGCTACTTTC GCTGTCACAA GGCAACATAG CCAAAGAGTA TCCTGTAAAA
    GTGCCCAGTG ACCTGCCTGC AGATTTCCAA GATTTCCTGC AAAAGTGTGT TTGCTTGGAA
    GACAAGGAAC GCTGGACTAC ACAGCAGTTA CTAAGTCACA GTTTCCTTAA CCCTCCACCT
    GAGAAGAGTC CTGTAACAGA GGAATGCTCA GATGATTATC CGGGTGACTG TACTGAGACT
    GTCATTCCCA GATCTTCAGT ATCTAATTCC ACATTCCATA TTGAGTCACA GAGACAGTTT
    TCTCGCTACT TCAATGAGTT TGAAGAATTA CAGATGCTTG GGAAAGGGGC ATTTGGTGCT
    GTCATTAAGG TTCAGAACAA ACTGGATGGC TGCTATTACG CTGTAAAACG GGTACAAATC
    AATCCTGCAA GTAAACAGTT CCGCAGAATT AAGGGGGAGG TAACATTGTT ATCTCGGCTC
    AACCATGAAA ACATTGTAAG GTACTATAAT GCATGGATTG AGAAACACGA GCATCCTACT
    ATTTCTCCTA AATGTGAAGC ACATGAGGAA AAGAAAGTGA CTGTGAAGCC AGAACAAATA
    GTAAAGAATG ATTTGGGATT GTTGGATGAT ATTGAGGAAA ACGCACCAGC ACCCATATTA
    ACCAGCTCTG TAGAATGGAG TACATCTTGC GAGCGATCAT CCAGTGCGCA CTGTACTAAT
    GGGGATCAAG ATTCAAGTGA TGATGATGAT GATGACGATG AAGAGGGAGT GTTTTCACCT
    TCGTTCCTGC CTGGGACAGA TTCTGACAGT GAGATCATAT TTGAGAATGA AGATGACATT
    AGTCCAGATC AGGATGAAGA ATATGACAAG AACAATGTAG AAGACCGCAA AGCTGGAGCC
    AATGAGATTG TGACTCAGGC AGTGCATTAT TTATATATTC AGATGGAATA TTGTGAGAAA
    AGCACCTTAC GGGACACGAT AGACCAAGGA CTGTATATGG ATGTTGGCCG CCTCTGGAGG
    CTGTTTCGTG AAATTTTGGA TGGATTGACC TATATACATG AGCAGGGAAT GATCCACAGA
    GACCTAAAGC CAGTCAATAT CTTTTTAGAT TCAGAAGATC ATGTCAAAAT AGGAGATTTT
    GGACTAGCTA CAGATCACCC AGCATCTTAT ACTGAATCAG CACAAGGGGA AGAGGATCAG
    GCGCATTCTG ACTTGGTTAA AGTCGACCCT GAAGATAGGG CCTCAACACC TGGTCGCAAT
    ACTCAAGGAA AACTCACTGG CATGGTTGGC ACTGCCTTGT ATGTAAGCCC TGAAGTCCAA
    GGAAACACCA AAGCTTCATA TAACCAGAAA GTGGACCTTT ACAGCCTGGG TATAATATTG
    TTTGAGATGT CCTACCGGCC CATGGACACA AGTTCTGAAC GTATCTCTGT CTTAAGCAGG
    CTTAGACAGG CCTTGATTGT GTTCCCAAGT GATTTTGAAA ACCCAGAAAA TGAAAAACAG
    AAAAAAGTGA TTACTTGGCT CCTGAACCAT GACCCAGCAT CCCGCCCCAC CGCTATGGAG
    TTGCTTAAGT GCGATCTCTT ACCCCCACCA CAACTAGAGG AGTCTGAACT CCATGAAGTC
    CTGCACCACA CCTTGGCCAA TATCGATGGG AAAGCGTACC GCACAATGAT GAGTCAAATC
    TTTGCCCAGC GCATTTCACC TGCTATGGAT TATACATATG ACAGTGATAT ATTGAAGGGT
    ATTTTCTCAG TCCGTTCTGC CAGACTACAT CATCATGTTT GTGAGGCAGT CTGCAGGATA
    TTTAAAAGAC ACGGGGCTGT GAAGTTACAT GCCCCACTGC TGATGCCACG AGGTAAAGCT
    GGGTTTGATA ACAGTGAATC TGCATGTTTT ATGGATCACA GTGGCATGCT GGTCACCCTT
    CCTTATAACC TCAGGATATC ATTTGCGCGG TTTGTTGCAA GGAATAATAT AACTAGTTTG
    AAAAGGTATA GCATTGAGAG AGTGTTTAGA CCACGCAAAC TGAACCGCTG CCATCCAAAG
    GAACTCACTG AGTGTGCATT TGATATAATC ACTTCTGCTG CAAACTGTTA CCTGCCCATT
    GCAGAAACAA TATACACCAT ATATGAAATT ATCCAGGAAT TTCCAGTACT GCAGGAAAGG
    AATTACAGTA TTTATGTGAA CCACACCTCG TTGTTGAAAG CCATTCTCCT TCACTGTGGC
    GTGCCCGAGG ATAAACTCAC TCAGGTGTAT AACATTCTGT ATGATGCAAT GATTGAGAAG
    CTGACAAAAA GAGAAGTGGA AGCCAAATTT TGTAATCTCT CCCTTTCAGA GAACAGTTTC
    CAATTGAGTC GCCTTTACCG GTTTATTGAG CAGAAAGGAG ACCTCCCAGA TTTGGAGGCA
    ATGATGGTCC CACTCACAAA GCAAAAAACA GGGGTCGGTG TGCTGGCAAA GCAAGGTTTG
    AAAGACTTGG AAGGAGTCAT TGGATTAGTG AAGAGGCTGG GCATTAAATT ACAGATTGTG
    GTTAACTTTG GACTTGTCTA CAAGATGCAG CAGCACAGTG GCATAGTCTT CCAGTTTGTG
    GCTTTTATCA AGCGGAGACA GAGGACTGTG CCTGAAATAC TTGCAGCTGG AGGCAGATAT
    GACCATTTGA TTTCCCAGTT TAGGGGACCT CAGACTGGAG CTATGGGATT ACCCTGTGCT
    GTAGGCGTTA GTATAGCAAT AGACAAGATA TCTGCTGCGC TTTCCACTGC AGAGGAACAT
    ATCAGCAGCT GTGATTTATT AGTCGTAAGC GCTGGGCAGA TGTCTCTGAA TAGAGCCATT
    GGGATAACAC AGAAGCTGTG GGCTGCTGGC ATTACTGCAG AGATCATGTA TGAGTGGTCT
    GAGTCTCAGG ATGAACTGCA GGATTATTGC AAGTGTTCGG GGATTTCGTA TGCTGCAATT
    ATTTCTGACA AAGAAGGAAA TCACATTAAG CTGAAAACAT TTGAGAAGGA AAGACAGAGT
    GAGAAGAGAA TATTGGAATC TGATCTAGTG GATCTCCTGG TGCAGAAACT TCGGATCAGA
    GTCCTTGAAG AAAGAAATAA CAGAGAAACG TCGGACAGTC TCTCAGTGCA AAATGTGAAG
    GGCTCGTTTA ATTCCCAGAG TCAGTTTGAG CCACACAGCG CAATAATTGT GCCAAGTGTC
    AGTGTTATAG CACCAGAAAA GCTATCTGCC AGTGCCAGGC GGAGATTTGA AACCCAGGTG
    CTAACAAAGC TTCAGACACT CATTACAAAC TTCCAGCAGA GAAATGGTGA AATTGAAATT
    TTAGCAGTTG ATCTGCCAAA AGAAACAATA CAGAACTTGT TAGCTTTAGA GTGCGCAGAT
    GAACAAGTCT TCAATGCATC AGTGAAAATG CTAATGTCTC GGCTGCCAAA ACAGAGATAC
    CTGAAATCAG TGTTTGATGA AATATACACC ATTAAAATCG AAAAGCGGGT TCCTGTGCTG
    GTACTATACA GTTATAAAGA TGATTATTAC AAGATCCTTT TTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012823581.1
    CDS107..5131
    Translation

    Target ORF information:

    RefSeq Version XM_012968127.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis eukaryotic translation initiation factor 2 alpha kinase 4 (eif2ak4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012968127.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGCAGGCG GCCGGGGAAA TGCACTTCTT CCTATTGAGC TCAGCCCGCA AGGGGGTCTG 
    CGCCCTACTC CGCCTGCCTG TGACCCCCCT GAAACTTATT CCCACCGGCA GGAGAATGAG
    CTGGAGGTCC TGGAATCCAT CTATAGTGGG GATTTCGCGG ATCTGCGCAA GAACTCTGCC
    TGGAAGGTAC AGCAGCCTCC AGAAGTCATC ATTGCCTTAC GTCCTCAGGG AAATGAGGTT
    CATGTCAAGG TGGATTTATG GGTAAAATGC CCTCAGAACT ATCCTGATGT TCCTCCTGAA
    ATAAAGCTGA AGAACATCCA GGGGTTGTCC AATGAAAATG TGAACTCTCT GACATCAAGC
    TTGGAAGATT TAGCAAAACA GCTTTGTGGA GAGGTAATGA TATACCAGCT TGCGGATCAT
    GTGCAGTGTT TTCTTACAGA GTATAACCAT CCACCTCCTA AATCCTTTCA TGAGGAGATG
    TTAAAAAACC TTCAGAAGCA GCAGGAGAAG CTTGCTCTTG AAGAAGAGAG ACGAATGCAA
    GAGATAAAGA TGAAAGAAGA GCAGACGAGA CGGGAAATCC TTAATGAAAT CCAGAAGCGA
    GAAGAAGAGA AAAAGGAAGA AAAAAGAAGG AAGGAAGTAG CAAAACAGGA ACGGATTGAA
    AACGCTAATC TAATGTGCCA AGAGAATGTA AATAAGATTA ATAATACAGG CAAGACAACA
    ACAGCTGCCT CGGATGGGTC ACTTCCTGAT CATGGAGGAA CCAACAGAAA TCGGCCACAT
    TCAGCAGGAC GCTCAAAGCG GGACCGACAG TATTCGGTGT GCCTCACTGA TGACTCAACC
    CCATTTGAAG TGTTAACCTT TGATATTGGA TGCAGTGGGC AAATTGTTGT TCACAGAGGA
    AAGTGCCTGG GGAAAGATGA GCTGCTGGGT AAATCTGTTT ACAATGGCTT GGAAACTGCT
    TCAGGACATT TTGTTGTAAT CTATGAATGG GTTCTCCAGT GGCAGAAAAA GATGGGCAAG
    TTTCTTACTA ATCAAGAAAA AGAAAAGATT GAAAAGTGTA AAAAACAGAT TCAGGGAACA
    GAAACCGAGT TCAGTTCTCT TATCAAACTG AGCCATCCAA ACATTGTGCA CTACCTCGCT
    ATGCGAATAA AAGAGAAAGA AGATTCCATT TCTGTCGGGA TCATAGCTGA ACATGTTAAT
    GGTACAAAGC TTTCCAGTTA CCTTGAGCAG GGTGTACCTG TTCCACTGGA TCAACTACGG
    CACTATACTG CCCAGCTGCT ATCGGCACTG GACTATTTGC ATTCTAATTC TGTGGTACAT
    AAAGTCATGT GCGCCTCCAG TGTTTTGGTG ACAGCTGACG GGAAAGTAAA ACTGACAGAC
    TATAGCCTTT CCAAACGCCT TGCTGACATC TGCAAAGAGG ACGTGTTTGA GCAAACAAAA
    GTTCGATTCA GTGAGGATGC TCTGCCTAGC AAGAGTGGCA AGAAAGGAGA TATATGGAAG
    CTCGGTCTCT TGCTACTTTC GCTGTCACAA GGCAACATAG CCAAAGAGTA TCCTGTAAAA
    GTGCCCAGTG ACCTGCCTGC AGATTTCCAA GATTTCCTGC AAAAGTGTGT TTGCTTGGAA
    GACAAGGAAC GCTGGACTAC ACAGCAGTTA CTAAGTCACA GTTTCCTTAA CCCTCCACCT
    GAGAAGAGTC CTGTAACAGA GGAATGCTCA GATGATTATC CGGGTGACTG TACTGAGACT
    GTCATTCCCA GATCTTCAGT ATCTAATTCC ACATTCCATA TTGAGTCACA GAGACAGTTT
    TCTCGCTACT TCAATGAGTT TGAAGAATTA CAGATGCTTG GGAAAGGGGC ATTTGGTGCT
    GTCATTAAGG TTCAGAACAA ACTGGATGGC TGCTATTACG CTGTAAAACG GGTACAAATC
    AATCCTGCAA GTAAACAGTT CCGCAGAATT AAGGGGGAGG TAACATTGTT ATCTCGGCTC
    AACCATGAAA ACATTGTAAG GTACTATAAT GCATGGATTG AGAAACACGA GCATCCTACT
    ATTTCTCCTA AATGTGAAGC ACATGAGGAA AAGAAAGTGA CTGTGAAGCC AGAACAAATA
    GTAAAGAATG ATTTGGGATT GTTGGATGAT ATTGAGGAAA ACGCACCAGC ACCCATATTA
    ACCAGCTCTG TAGAATGGAG TACATCTTGC GAGCGATCAT CCAGTGCGCA CTGTACTAAT
    GGGGATCAAG ATTCAAGTGA TGATGATGAT GATGACGATG AAGAGGGAGT GTTTTCACCT
    TCGTTCCTGC CTGGGACAGA TTCTGACAGT GAGATCATAT TTGAGAATGA AGATGACATT
    AGTCCAGATC AGGATGAAGA ATATGACAAG AACAATGTAG AAGACCGCAA AGCTGGAGCC
    AATGAGATTG TGACTCAGGC AGTGCATTAT TTATATATTC AGATGGAATA TTGTGAGAAA
    AGCACCTTAC GGGACACGAT AGACCAAGGA CTGTATATGG ATGTTGGCCG CCTCTGGAGG
    CTGTTTCGTG AAATTTTGGA TGGATTGACC TATATACATG AGCAGGGAAT GATCCACAGA
    GACCTAAAGC CAGTCAATAT CTTTTTAGAT TCAGAAGATC ATGTCAAAAT AGGAGATTTT
    GGACTAGCTA CAGATCACCC AGCATCTTAT ACTGAATCAG CACAAGGGGA AGAGGATCAG
    GCGCATTCTG ACTTGGTTAA AGTCGACCCT GAAGATAGGG CCTCAACACC TGGTCGCAAT
    ACTCAAGGAA AACTCACTGG CATGGTTGGC ACTGCCTTGT ATGTAAGCCC TGAAGTCCAA
    GGAAACACCA AAGCTTCATA TAACCAGAAA GTGGACCTTT ACAGCCTGGG TATAATATTG
    TTTGAGATGT CCTACCGGCC CATGGACACA AGTTCTGAAC GTATCTCTGT CTTAAGCAGG
    CTTAGACAGG CCTTGATTGT GTTCCCAAGT GATTTTGAAA ACCCAGAAAA TGAAAAACAG
    AAAAAAGTGA TTACTTGGCT CCTGAACCAT GACCCAGCAT CCCGCCCCAC CGCTATGGAG
    TTGCTTAAGT GCGATCTCTT ACCCCCACCA CAACTAGAGG AGTCTGAACT CCATGAAGTC
    CTGCACCACA CCTTGGCCAA TATCGATGGG AAAGCGTACC GCACAATGAT GAGTCAAATC
    TTTGCCCAGC GCATTTCACC TGCTATGGAT TATACATATG ACAGTGATAT ATTGAAGGGT
    ATTTTCTCAG TCCGTTCTGC CAGACTACAT CATCATGTTT GTGAGGCAGT CTGCAGGATA
    TTTAAAAGAC ACGGGGCTGT GAAGTTACAT GCCCCACTGC TGATGCCACG AGGTAAAGCT
    GGGTTTGATA ACAGTGAATC TGCATGTTTT ATGGATCACA GTGGCATGCT GGTCACCCTT
    CCTTATAACC TCAGGATATC ATTTGCGCGG TTTGTTGCAA GGAATAATAT AACTAGTTTG
    AAAAGGTATA GCATTGAGAG AGTGTTTAGA CCACGCAAAC TGAACCGCTG CCATCCAAAG
    GAACTCACTG AGTGTGCATT TGATATAATC ACTTCTGCTG CAAACTGTTA CCTGCCCATT
    GCAGAAACAA TATACACCAT ATATGAAATT ATCCAGGAAT TTCCAGTACT GCAGGAAAGG
    AATTACAGTA TTTATGTGAA CCACACCTCG TTGTTGAAAG CCATTCTCCT TCACTGTGGC
    GTGCCCGAGG ATAAACTCAC TCAGGTGTAT AACATTCTGT ATGATGCAAT GATTGAGAAG
    CTGACAAAAA GAGAAGTGGA AGCCAAATTT TGTAATCTCT CCCTTTCAGA GAACAGTTTC
    CAATTGAGTC GCCTTTACCG GTTTATTGAG CAGAAAGGAG ACCTCCCAGA TTTGGAGGCA
    ATGATGGTCC CACTCACAAA GCAAAAAACA GGGGTCGGTG TGCTGGCAAA GCAAGGTTTG
    AAAGACTTGG AAGGAGTCAT TGGATTAGTG AAGAGGCTGG GCATTAAATT ACAGATTGTG
    GTTAACTTTG GACTTGTCTA CAAGATGCAG CAGCACAGTG GCATAGTCTT CCAGTTTGTG
    GCTTTTATCA AGCGGAGACA GAGGACTGTG CCTGAAATAC TTGCAGCTGG AGGCAGATAT
    GACCATTTGA TTTCCCAGTT TAGGGGACCT CAGACTGGAG CTATGGGATT ACCCTGTGCT
    GTAGGCGTTA GTATAGCAAT AGACAAGATA TCTGCTGCGC TTTCCACTGC AGAGGAACAT
    ATCAGCAGCT GTGATTTATT AGTCGTAAGC GCTGGGCAGA TGTCTCTGAA TAGAGCCATT
    GGGATAACAC AGAAGCTGTG GGCTGCTGGC ATTACTGCAG AGATCATGTA TGAGTGGTCT
    GAGTCTCAGG ATGAACTGCA GGATTATTGC AAGTGTTCGG GGATTTCGTA TGCTGCAATT
    ATTTCTGACA AAGAAGGAAA TCACATTAAG CTGAAAACAT TTGAGAAGGA AAGACAGAGT
    GAGAAGAGAA TATTGGAATC TGATCTAGTG GATCTCCTGG TGCAGAAACT TCGGATCAGA
    GTCCTTGAAG AAAGAAATAA CAGAGAAACG TCGGACAGTC TCTCAGTGCA AAATGTGAAG
    GGCTCGTTTA ATTCCCAGAG TCAGTTTGAG CCACACAGCG CAATAATTGT GCCAAGTGTC
    AGTGTTATAG CACCAGAAAA GCTATCTGCC AGTGCCAGGC GGAGATTTGA AACCCAGGTG
    CTAACAAAGC TTCAGACACT CATTACAAAC TTCCAGCAGA GAAATGGTGA AATTGAAATT
    TTAGCAGTTG ATCTGCCAAA AGAAACAATA CAGAACTTGT TAGCTTTAGA GTGCGCAGAT
    GAACAAGTCT TCAATGCATC AGTGAAAATG CTAATGTCTC GGCTGCCAAA ACAGAGATAC
    CTGAAATCAG TGTTTGATGA AATATACACC ATTAAAATCG AAAAGCGGGT TCCTGTGCTG
    GTACTATACA GTTATAAAGA TGATTATTAC AAGATCCTTT TTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32795
    Clone ID Related Accession (Same CDS sequence) XM_012968128.3 , XM_012968128.2
    Accession Version XM_012968128.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4992bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product eIF-2-alpha kinase GCN2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012968128.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGCAGGCG GCCGGGGAAA TGCACTTCTT CCTATTGAGC TCAGCCCGCA AGGGGGTCTG 
    CGCCCTACTC CGCCTGCCTG TGACCCCCCT GAAACTTATT CCCACCGGCA GGAGAATGAG
    CTGGAGGTCC TGGAATCCAT CTATAGTGGG GATTTCGCGG ATCTGCGCAA GAACTCTGCC
    TGGAAGGTAC AGCAGCCTCC AGAAGTCATC ATTGCCTTAC GTCCTCAGGG AAATGAGGTT
    CATGTCAAGG TGGATTTATG GGTAAAATGC CCTCAGAACT ATCCTGATGT TCCTCCTGAA
    ATAAAGCTGA AGAACATCCA GGGGTTGTCC AATGAAAATG TGAACTCTCT GACATCAAGC
    TTGGAAGATT TAGCAAAACA GCTTTGTGGA GAGGTAATGA TATACCAGCT TGCGGATCAT
    GTGCAGTGTT TTCTTACAGA GTATAACCAT CCACCTCCTA AATCCTTTCA TGAGGAGATG
    TTAAAAAACC TTCAGAAGCA GCAGGAGAAG CTTGCTCTTG AAGAAGAGAG ACGAATGCAA
    GAGATAAAGA TGAAAGAAGA GCAGACGAGA CGGGAAATCC TTAATGAAAT CCAGAAGCGA
    GAAGAAGAGA AAAAGGAAGA AAAAAGAAGG AAGGAAGTAG CAAAACAGGA ACGGATTGAA
    AACGCTAATC TAATGTGCCA AGAGAATGTA AATAAGATTA ATAATACAGG CAAGACAACA
    ACAGCTGCCT CGGATGGGTC ACTTCCTGAT CATGGAGGAA CCAACAGAAA TCGGCCACAT
    TCAGCAGGAC GCTCAAAGCG GGACCGACAG TATTCGGTGT GCCTCACTGA TGACTCAACC
    CCATTTGAAG TGTTAACCTT TGATATTGGA TGCAGTGGGC AAATTGTTGT TCACAGAGGA
    AAGTGCCTGG GGAAAGATGA GCTGCTGGGT AAATCTGTTT ACAATGGCTT GGAAACTGCT
    TCAGGACATT TTGTTGTAAT CTATGAATGG GTTCTCCAGT GGCAGAAAAA GATGGGCAAG
    TTTCTTACTA ATCAAGAAAA AGAAAAGATT GAAAAGTGTA AAAAACAGAT TCAGGGAACA
    GAAACCGAGT TCAGTTCTCT TATCAAACTG AGCCATCCAA ACATTGTGCA CTACCTCGCT
    ATGCGAATAA AAGAGAAAGA AGATTCCATT TCTGTCGGGA TCATAGCTGA ACATGTTAAT
    GGTACAAAGC TTTCCAGTTA CCTTGAGCAG GGTGTACCTG TTCCACTGGA TCAACTACGG
    CACTATACTG CCCAGCTGCT ATCGGCACTG GACTATTTGC ATTCTAATTC TGTGGTACAT
    AAAGTCATGT GCGCCTCCAG TGTTTTGGTG ACAGCTGACG GGAAAGTAAA ACTGACAGAC
    TATAGCCTTT CCAAACGCCT TGCTGACATC TGCAAAGAGG ACGTGTTTGA GCAAACAAAA
    GTTCGATTCA GTGAGGATGC TCTGCCTAGC AAGAGTGGCA AGAAAGGAGA TATATGGAAG
    CTCGGTCTCT TGCTACTTTC GCTGTCACAA GGCAACATAG CCAAAGAGTA TCCTGTAAAA
    GTGCCCAGTG ACCTGCCTGC AGATTTCCAA GATTTCCTGC AAAAGTGTGT TTGCTTGGAA
    GACAAGGAAC GCTGGACTAC ACAGCAGTTA CTAAGTCACA GTTTCCTTAA CCCTCCACCT
    GAGAAGAGTC CTGTAACAGA GGAATGCTCA GATGATTATC CGGGTGACTG TACTGAGACT
    GTCATTCCCA GATCTTCAGT ATCTAATTCC ACATTCCATA TTGAGTCACA GAGACAGTTT
    TCTCGCTACT TCAATGAGTT TGAAGAATTA CAGATGCTTG GGAAAGGGGC ATTTGGTGCT
    GTCATTAAGG TTCAGAACAA ACTGGATGGC TGCTATTACG CTGTAAAACG GGTACAAATC
    AATCCTGCAA GTAAACAGTT CCGCAGAATT AAGGGGGAGG TAACATTGTT ATCTCGGCTC
    AACCATGAAA ACATTGTAAG GTACTATAAT GCATGGATTG AGAAACACGA GCATCCTACT
    ATTTCTCCTA AATGTGAAGC ACATGAGGAA AAGAAAGTGA CTGTGAAGCC AGAACAAATA
    GTAAAGAATG ATTTGGGATT GTTGGATGAT ATTGAGGAAA ACGCACCAGC ACCCATATTA
    ACCAGCTCTG TAGAATGGAG TACATCTTGC GAGCGATCAT CCAGTGCGCA CTGTACTAAT
    GGGGATCAAG ATTCAAGTGA TGATGATGAT GATGACGATG AAGAGGGAGT GTTTTCACCT
    TCGTTCCTGC CTGGGACAGA TTCTGACAGT GAGATCATAT TTGAGAATGA AGATGACATT
    AGTCCAGATC AGGATGAAGA ATATGACAAG AACAATGTAG AAGACCGCAA AGCTGGAGCC
    AATGAGATTG TGACTCAGGC AGTGCATTAT TTATATATTC AGATGGAATA TTGTGAGAAA
    AGCACCTTAC GGGACACGAT AGACCAAGGA CTGTATATGG ATGTTGGCCG CCTCTGGAGG
    CTGTTTCGTG AAATTTTGGA TGGATTGACC TATATACATG AGCAGGGAAT GATCCACAGA
    GACCTAAAGC CAGTCAATAT CTTTTTAGAT TCAGAAGATC ATGTCAAAAT AGGAGATTTT
    GGACTAGCTA CAGATCACCC AGCATCTTAT ACTGAATCAG CACAAGGGGA AGAGGATCAG
    GCGCATTCTG ACTTGGTTAA AGTCGACCCT GAAGGAAAAC TCACTGGCAT GGTTGGCACT
    GCCTTGTATG TAAGCCCTGA AGTCCAAGGA AACACCAAAG CTTCATATAA CCAGAAAGTG
    GACCTTTACA GCCTGGGTAT AATATTGTTT GAGATGTCCT ACCGGCCCAT GGACACAAGT
    TCTGAACGTA TCTCTGTCTT AAGCAGGCTT AGACAGGCCT TGATTGTGTT CCCAAGTGAT
    TTTGAAAACC CAGAAAATGA AAAACAGAAA AAAGTGATTA CTTGGCTCCT GAACCATGAC
    CCAGCATCCC GCCCCACCGC TATGGAGTTG CTTAAGTGCG ATCTCTTACC CCCACCACAA
    CTAGAGGAGT CTGAACTCCA TGAAGTCCTG CACCACACCT TGGCCAATAT CGATGGGAAA
    GCGTACCGCA CAATGATGAG TCAAATCTTT GCCCAGCGCA TTTCACCTGC TATGGATTAT
    ACATATGACA GTGATATATT GAAGGGTATT TTCTCAGTCC GTTCTGCCAG ACTACATCAT
    CATGTTTGTG AGGCAGTCTG CAGGATATTT AAAAGACACG GGGCTGTGAA GTTACATGCC
    CCACTGCTGA TGCCACGAGG TAAAGCTGGG TTTGATAACA GTGAATCTGC ATGTTTTATG
    GATCACAGTG GCATGCTGGT CACCCTTCCT TATAACCTCA GGATATCATT TGCGCGGTTT
    GTTGCAAGGA ATAATATAAC TAGTTTGAAA AGGTATAGCA TTGAGAGAGT GTTTAGACCA
    CGCAAACTGA ACCGCTGCCA TCCAAAGGAA CTCACTGAGT GTGCATTTGA TATAATCACT
    TCTGCTGCAA ACTGTTACCT GCCCATTGCA GAAACAATAT ACACCATATA TGAAATTATC
    CAGGAATTTC CAGTACTGCA GGAAAGGAAT TACAGTATTT ATGTGAACCA CACCTCGTTG
    TTGAAAGCCA TTCTCCTTCA CTGTGGCGTG CCCGAGGATA AACTCACTCA GGTGTATAAC
    ATTCTGTATG ATGCAATGAT TGAGAAGCTG ACAAAAAGAG AAGTGGAAGC CAAATTTTGT
    AATCTCTCCC TTTCAGAGAA CAGTTTCCAA TTGAGTCGCC TTTACCGGTT TATTGAGCAG
    AAAGGAGACC TCCCAGATTT GGAGGCAATG ATGGTCCCAC TCACAAAGCA AAAAACAGGG
    GTCGGTGTGC TGGCAAAGCA AGGTTTGAAA GACTTGGAAG GAGTCATTGG ATTAGTGAAG
    AGGCTGGGCA TTAAATTACA GATTGTGGTT AACTTTGGAC TTGTCTACAA GATGCAGCAG
    CACAGTGGCA TAGTCTTCCA GTTTGTGGCT TTTATCAAGC GGAGACAGAG GACTGTGCCT
    GAAATACTTG CAGCTGGAGG CAGATATGAC CATTTGATTT CCCAGTTTAG GGGACCTCAG
    ACTGGAGCTA TGGGATTACC CTGTGCTGTA GGCGTTAGTA TAGCAATAGA CAAGATATCT
    GCTGCGCTTT CCACTGCAGA GGAACATATC AGCAGCTGTG ATTTATTAGT CGTAAGCGCT
    GGGCAGATGT CTCTGAATAG AGCCATTGGG ATAACACAGA AGCTGTGGGC TGCTGGCATT
    ACTGCAGAGA TCATGTATGA GTGGTCTGAG TCTCAGGATG AACTGCAGGA TTATTGCAAG
    TGTTCGGGGA TTTCGTATGC TGCAATTATT TCTGACAAAG AAGGAAATCA CATTAAGCTG
    AAAACATTTG AGAAGGAAAG ACAGAGTGAG AAGAGAATAT TGGAATCTGA TCTAGTGGAT
    CTCCTGGTGC AGAAACTTCG GATCAGAGTC CTTGAAGAAA GAAATAACAG AGAAACGTCG
    GACAGTCTCT CAGTGCAAAA TGTGAAGGGC TCGTTTAATT CCCAGAGTCA GTTTGAGCCA
    CACAGCGCAA TAATTGTGCC AAGTGTCAGT GTTATAGCAC CAGAAAAGCT ATCTGCCAGT
    GCCAGGCGGA GATTTGAAAC CCAGGTGCTA ACAAAGCTTC AGACACTCAT TACAAACTTC
    CAGCAGAGAA ATGGTGAAAT TGAAATTTTA GCAGTTGATC TGCCAAAAGA AACAATACAG
    AACTTGTTAG CTTTAGAGTG CGCAGATGAA CAAGTCTTCA ATGCATCAGT GAAAATGCTA
    ATGTCTCGGC TGCCAAAACA GAGATACCTG AAATCAGTGT TTGATGAAAT ATACACCATT
    AAAATCGAAA AGCGGGTTCC TGTGCTGGTA CTATACAGTT ATAAAGATGA TTATTACAAG
    ATCCTTTTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012823582.1
    CDS106..5097
    Translation

    Target ORF information:

    RefSeq Version XM_012968128.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis eukaryotic translation initiation factor 2 alpha kinase 4 (eif2ak4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012968128.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGCAGGCG GCCGGGGAAA TGCACTTCTT CCTATTGAGC TCAGCCCGCA AGGGGGTCTG 
    CGCCCTACTC CGCCTGCCTG TGACCCCCCT GAAACTTATT CCCACCGGCA GGAGAATGAG
    CTGGAGGTCC TGGAATCCAT CTATAGTGGG GATTTCGCGG ATCTGCGCAA GAACTCTGCC
    TGGAAGGTAC AGCAGCCTCC AGAAGTCATC ATTGCCTTAC GTCCTCAGGG AAATGAGGTT
    CATGTCAAGG TGGATTTATG GGTAAAATGC CCTCAGAACT ATCCTGATGT TCCTCCTGAA
    ATAAAGCTGA AGAACATCCA GGGGTTGTCC AATGAAAATG TGAACTCTCT GACATCAAGC
    TTGGAAGATT TAGCAAAACA GCTTTGTGGA GAGGTAATGA TATACCAGCT TGCGGATCAT
    GTGCAGTGTT TTCTTACAGA GTATAACCAT CCACCTCCTA AATCCTTTCA TGAGGAGATG
    TTAAAAAACC TTCAGAAGCA GCAGGAGAAG CTTGCTCTTG AAGAAGAGAG ACGAATGCAA
    GAGATAAAGA TGAAAGAAGA GCAGACGAGA CGGGAAATCC TTAATGAAAT CCAGAAGCGA
    GAAGAAGAGA AAAAGGAAGA AAAAAGAAGG AAGGAAGTAG CAAAACAGGA ACGGATTGAA
    AACGCTAATC TAATGTGCCA AGAGAATGTA AATAAGATTA ATAATACAGG CAAGACAACA
    ACAGCTGCCT CGGATGGGTC ACTTCCTGAT CATGGAGGAA CCAACAGAAA TCGGCCACAT
    TCAGCAGGAC GCTCAAAGCG GGACCGACAG TATTCGGTGT GCCTCACTGA TGACTCAACC
    CCATTTGAAG TGTTAACCTT TGATATTGGA TGCAGTGGGC AAATTGTTGT TCACAGAGGA
    AAGTGCCTGG GGAAAGATGA GCTGCTGGGT AAATCTGTTT ACAATGGCTT GGAAACTGCT
    TCAGGACATT TTGTTGTAAT CTATGAATGG GTTCTCCAGT GGCAGAAAAA GATGGGCAAG
    TTTCTTACTA ATCAAGAAAA AGAAAAGATT GAAAAGTGTA AAAAACAGAT TCAGGGAACA
    GAAACCGAGT TCAGTTCTCT TATCAAACTG AGCCATCCAA ACATTGTGCA CTACCTCGCT
    ATGCGAATAA AAGAGAAAGA AGATTCCATT TCTGTCGGGA TCATAGCTGA ACATGTTAAT
    GGTACAAAGC TTTCCAGTTA CCTTGAGCAG GGTGTACCTG TTCCACTGGA TCAACTACGG
    CACTATACTG CCCAGCTGCT ATCGGCACTG GACTATTTGC ATTCTAATTC TGTGGTACAT
    AAAGTCATGT GCGCCTCCAG TGTTTTGGTG ACAGCTGACG GGAAAGTAAA ACTGACAGAC
    TATAGCCTTT CCAAACGCCT TGCTGACATC TGCAAAGAGG ACGTGTTTGA GCAAACAAAA
    GTTCGATTCA GTGAGGATGC TCTGCCTAGC AAGAGTGGCA AGAAAGGAGA TATATGGAAG
    CTCGGTCTCT TGCTACTTTC GCTGTCACAA GGCAACATAG CCAAAGAGTA TCCTGTAAAA
    GTGCCCAGTG ACCTGCCTGC AGATTTCCAA GATTTCCTGC AAAAGTGTGT TTGCTTGGAA
    GACAAGGAAC GCTGGACTAC ACAGCAGTTA CTAAGTCACA GTTTCCTTAA CCCTCCACCT
    GAGAAGAGTC CTGTAACAGA GGAATGCTCA GATGATTATC CGGGTGACTG TACTGAGACT
    GTCATTCCCA GATCTTCAGT ATCTAATTCC ACATTCCATA TTGAGTCACA GAGACAGTTT
    TCTCGCTACT TCAATGAGTT TGAAGAATTA CAGATGCTTG GGAAAGGGGC ATTTGGTGCT
    GTCATTAAGG TTCAGAACAA ACTGGATGGC TGCTATTACG CTGTAAAACG GGTACAAATC
    AATCCTGCAA GTAAACAGTT CCGCAGAATT AAGGGGGAGG TAACATTGTT ATCTCGGCTC
    AACCATGAAA ACATTGTAAG GTACTATAAT GCATGGATTG AGAAACACGA GCATCCTACT
    ATTTCTCCTA AATGTGAAGC ACATGAGGAA AAGAAAGTGA CTGTGAAGCC AGAACAAATA
    GTAAAGAATG ATTTGGGATT GTTGGATGAT ATTGAGGAAA ACGCACCAGC ACCCATATTA
    ACCAGCTCTG TAGAATGGAG TACATCTTGC GAGCGATCAT CCAGTGCGCA CTGTACTAAT
    GGGGATCAAG ATTCAAGTGA TGATGATGAT GATGACGATG AAGAGGGAGT GTTTTCACCT
    TCGTTCCTGC CTGGGACAGA TTCTGACAGT GAGATCATAT TTGAGAATGA AGATGACATT
    AGTCCAGATC AGGATGAAGA ATATGACAAG AACAATGTAG AAGACCGCAA AGCTGGAGCC
    AATGAGATTG TGACTCAGGC AGTGCATTAT TTATATATTC AGATGGAATA TTGTGAGAAA
    AGCACCTTAC GGGACACGAT AGACCAAGGA CTGTATATGG ATGTTGGCCG CCTCTGGAGG
    CTGTTTCGTG AAATTTTGGA TGGATTGACC TATATACATG AGCAGGGAAT GATCCACAGA
    GACCTAAAGC CAGTCAATAT CTTTTTAGAT TCAGAAGATC ATGTCAAAAT AGGAGATTTT
    GGACTAGCTA CAGATCACCC AGCATCTTAT ACTGAATCAG CACAAGGGGA AGAGGATCAG
    GCGCATTCTG ACTTGGTTAA AGTCGACCCT GAAGGAAAAC TCACTGGCAT GGTTGGCACT
    GCCTTGTATG TAAGCCCTGA AGTCCAAGGA AACACCAAAG CTTCATATAA CCAGAAAGTG
    GACCTTTACA GCCTGGGTAT AATATTGTTT GAGATGTCCT ACCGGCCCAT GGACACAAGT
    TCTGAACGTA TCTCTGTCTT AAGCAGGCTT AGACAGGCCT TGATTGTGTT CCCAAGTGAT
    TTTGAAAACC CAGAAAATGA AAAACAGAAA AAAGTGATTA CTTGGCTCCT GAACCATGAC
    CCAGCATCCC GCCCCACCGC TATGGAGTTG CTTAAGTGCG ATCTCTTACC CCCACCACAA
    CTAGAGGAGT CTGAACTCCA TGAAGTCCTG CACCACACCT TGGCCAATAT CGATGGGAAA
    GCGTACCGCA CAATGATGAG TCAAATCTTT GCCCAGCGCA TTTCACCTGC TATGGATTAT
    ACATATGACA GTGATATATT GAAGGGTATT TTCTCAGTCC GTTCTGCCAG ACTACATCAT
    CATGTTTGTG AGGCAGTCTG CAGGATATTT AAAAGACACG GGGCTGTGAA GTTACATGCC
    CCACTGCTGA TGCCACGAGG TAAAGCTGGG TTTGATAACA GTGAATCTGC ATGTTTTATG
    GATCACAGTG GCATGCTGGT CACCCTTCCT TATAACCTCA GGATATCATT TGCGCGGTTT
    GTTGCAAGGA ATAATATAAC TAGTTTGAAA AGGTATAGCA TTGAGAGAGT GTTTAGACCA
    CGCAAACTGA ACCGCTGCCA TCCAAAGGAA CTCACTGAGT GTGCATTTGA TATAATCACT
    TCTGCTGCAA ACTGTTACCT GCCCATTGCA GAAACAATAT ACACCATATA TGAAATTATC
    CAGGAATTTC CAGTACTGCA GGAAAGGAAT TACAGTATTT ATGTGAACCA CACCTCGTTG
    TTGAAAGCCA TTCTCCTTCA CTGTGGCGTG CCCGAGGATA AACTCACTCA GGTGTATAAC
    ATTCTGTATG ATGCAATGAT TGAGAAGCTG ACAAAAAGAG AAGTGGAAGC CAAATTTTGT
    AATCTCTCCC TTTCAGAGAA CAGTTTCCAA TTGAGTCGCC TTTACCGGTT TATTGAGCAG
    AAAGGAGACC TCCCAGATTT GGAGGCAATG ATGGTCCCAC TCACAAAGCA AAAAACAGGG
    GTCGGTGTGC TGGCAAAGCA AGGTTTGAAA GACTTGGAAG GAGTCATTGG ATTAGTGAAG
    AGGCTGGGCA TTAAATTACA GATTGTGGTT AACTTTGGAC TTGTCTACAA GATGCAGCAG
    CACAGTGGCA TAGTCTTCCA GTTTGTGGCT TTTATCAAGC GGAGACAGAG GACTGTGCCT
    GAAATACTTG CAGCTGGAGG CAGATATGAC CATTTGATTT CCCAGTTTAG GGGACCTCAG
    ACTGGAGCTA TGGGATTACC CTGTGCTGTA GGCGTTAGTA TAGCAATAGA CAAGATATCT
    GCTGCGCTTT CCACTGCAGA GGAACATATC AGCAGCTGTG ATTTATTAGT CGTAAGCGCT
    GGGCAGATGT CTCTGAATAG AGCCATTGGG ATAACACAGA AGCTGTGGGC TGCTGGCATT
    ACTGCAGAGA TCATGTATGA GTGGTCTGAG TCTCAGGATG AACTGCAGGA TTATTGCAAG
    TGTTCGGGGA TTTCGTATGC TGCAATTATT TCTGACAAAG AAGGAAATCA CATTAAGCTG
    AAAACATTTG AGAAGGAAAG ACAGAGTGAG AAGAGAATAT TGGAATCTGA TCTAGTGGAT
    CTCCTGGTGC AGAAACTTCG GATCAGAGTC CTTGAAGAAA GAAATAACAG AGAAACGTCG
    GACAGTCTCT CAGTGCAAAA TGTGAAGGGC TCGTTTAATT CCCAGAGTCA GTTTGAGCCA
    CACAGCGCAA TAATTGTGCC AAGTGTCAGT GTTATAGCAC CAGAAAAGCT ATCTGCCAGT
    GCCAGGCGGA GATTTGAAAC CCAGGTGCTA ACAAAGCTTC AGACACTCAT TACAAACTTC
    CAGCAGAGAA ATGGTGAAAT TGAAATTTTA GCAGTTGATC TGCCAAAAGA AACAATACAG
    AACTTGTTAG CTTTAGAGTG CGCAGATGAA CAAGTCTTCA ATGCATCAGT GAAAATGCTA
    ATGTCTCGGC TGCCAAAACA GAGATACCTG AAATCAGTGT TTGATGAAAT ATACACCATT
    AAAATCGAAA AGCGGGTTCC TGTGCTGGTA CTATACAGTT ATAAAGATGA TTATTACAAG
    ATCCTTTTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa45985
    Clone ID Related Accession (Same CDS sequence) XM_018096178.1
    Accession Version XM_018096178.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4974bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product eIF-2-alpha kinase GCN2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCAGGCG GCCGGGGAAA TGCACTTCTT CCTATTGAGC TCAGCCCGCA AGGGGGTCTG 
    CGCCCTACTC CGCCTGCCTG TGACCCCCCT GAAACTTATT CCCACCGGCA GGAGAATGAG
    CTGGAGGTCC TGGAATCCAT CTATAGTGGG GATTTCGCGG ATCTGCGCAA GAACTCTGCC
    TGGAAGGTAC AGCAGCCTCC AGAAGTCATC ATTGCCTTAC GTCCTCAGGG AAATGAGGTT
    CATGTCAAGG TGGATTTATG GGTAAAATGC CCTCAGAACT ATCCTGATGT TCCTCCTGAA
    ATAAAGCTGA AGAACATCCA GGGGTTGTCC AATGAAAATG TGAACTCTCT GACATCAAGC
    TTGGAAGATT TAGCAAAACA GCTTTGTGGA GAGGTAATGA TATACCAGCT TGCGGATCAT
    GTGCAGTGTT TTCTTACAGA GTATAACCAT CCACCTCCTA AATCCTTTCA TGAGGAGATG
    TTAAAAAACC TTCAGAAGCA GCAGGAGAAG CTTGCTCTTG AAGAAGAGAG ACGAATGCAA
    GAGATAAAGA TGAAAGAAGA GCAGACGAGA CGGGAAATCC TTAATGAAAT CCAGAAGCGA
    GAAGAAGAGA AAAAGGAAGA AAAAAGAAGG AAGGAAGTAG CAAAACAGGA ACGGATTGAA
    AACGCTAATC TAATGTGCCA AGAGAATGTA AATAAGATTA ATAATACAGG CAAGACAACA
    ACAGCTGCCT CGGATGGGTC ACTTCCTGAT CATGGAGGAA CCAACAGAAA TCGGCCACAT
    TCAGCAGGAC GCTCAAAGCG GGACCGACAG TATTCGGTGT GCCTCACTGA TGACTCAACC
    CCATTTGAAG TGTTAACCTT TGATATTGGA TGCAGTGGGC AAATTGTTGT TCACAGAGGA
    AAGTGCCTGG GGAAAGATGA GCTGCTGGGT AAATCTGTTT ACAATGGCTT GGAAACTGCT
    TCAGGACATT TTGTTGTAAT CTATGAATGG GTTCTCCAGT GGCAGAAAAA GATGGGCAAG
    TTTCTTACTA ATCAAGAAAA AGAAAAGATT GAAAAGTGTA AAAAACAGAT TCAGGGAACA
    GAAACCGAGT TCAGTTCTCT TATCAAACTG AGCCATCCAA ACATTGTGCA CTACCTCGCT
    ATGCGAATAA AAGAGAAAGA AGATTCCATT TCTGTCGGGA TCATAGCTGA ACATGTTAAT
    GGTACAAAGC TTTCCAGTTA CCTTGAGCAG GGTGTACCTG TTCCACTGGA TCAACTACGG
    CACTATACTG CCCAGCTGCT ATCGGCACTG GACTATTTGC ATTCTAATTC TGTGGTACAT
    AAAGTCATGT GCGCCTCCAG TGTTTTGGTG ACAGCTGACG GGAAAGTAAA ACTGACAGAC
    TATAGCCTTT CCAAACGCCT TGCTGACATC TGCAAAGAGG ACGTGTTTGA GCAAACAAAA
    GTTCGATTCA GTGAGGATGC TCTGCCTAGC AAGAGTGGCA AGAAAGGAGA TATATGGAAG
    CTCGGTCTCT TGCTACTTTC GCTGTCACAA GGCAACATAG CCAAAGAGTA TCCTGTAAAA
    GTGCCCAGTG ACCTGCCTGC AGATTTCCAA GATTTCCTGC AAAAGTGTGT TTGCTTGGAA
    GACAAGGAAC GCTGGACTAC ACAGCAGTTA CTAAGTCACA GTTTCCTTAA CCCTCCACCT
    GAGAAGAGTC CTGTAACAGA GGAATGCTCA GATGATTATC CGGGTGACTG TACTGAGACT
    GTCATTCCCA GATCTTCAGT ATCTAATTCC ACATTCCATA TTGAGTCACA GAGACAGTTT
    TCTCGCTACT TCAATGAGTT TGAAGAATTA CAGATGCTTG GGAAAGGGGC ATTTGGTGCT
    GTCATTAAGG TTCAGAACAA ACTGGATGGC TGCTATTACG CTGTAAAACG GGTACAAATC
    AATCCTGCAA GTAAACAGTT CCGCAGAATT AAGGGGGAGG TAACATTGTT ATCTCGGCTC
    AACCATGAAA ACATTGTAAG GTACTATAAT GCATGGATTG AGAAACACGA GCATCCTACT
    ATTTCTCCTA AATGTGAAGC ACATGAGGAA AAGAAAGTGA CTGTGAAGCC AGAACAAATA
    GTAAAGAATG ATTTGGGATT GTTGGATGAT ATTGAGGAAA ACGCACCAGC ACCCATATTA
    ACCAGCTCTG TAGAATGGAG TACATCTTGC GAGCGATCAT CCAGTGCGCA CTGTACTAAT
    GGGGATCAAG ATTCAAGTGA TGATGATGAT GATGACGATG AAGAGGGAGT GTTTTCACCT
    TCGTTCCTGC CTGGGACAGA TTCTGACAGT GAGATCATAT TTGAGAATGA AGATGACATT
    AGTCCAGATC AGGATGAAGA ATATGACAAG AACAATGTAG AAGACCGCAA AGCTGGAGCC
    AATGAGATTG TGACTCAGGC AGTGCATTAT TTATATATTC AGATGGAATA TTGTGAGAAA
    AGCACCTTAC GGGACACGAT AGACCAAGGA CTGTATATGG ATGTTGGCCG CCTCTGGAGG
    CTGTTTCGTG AAATTTTGGA TGGATTGACC TATATACATG AGCAGGGAAT GATCCACAGA
    GACCTAAAGC CAGTCAATAT CTTTTTAGAT TCAGAAGATC ATGTCAAAAT AGGAGATTTT
    GGACTAGCTA CAGATCACCC AGCATCTTAT ACTGAATCAG CACAAGGGGA AGAGGATCAG
    GCGCATTCTG ACTTGGTTAA AGTCGACCCT GAAGATAGGG CCTCAACACC TGGTCGCAAT
    ACTCAAGGAA AACTCACTGG CATGGTTGGC ACTGCCTTGT ATGTAAGCCC TGAAGTCCAA
    GGAAACACCA AAGCTTCATA TAACCAGAAA GTGGACCTTT ACAGCCTGGG TATAATATTG
    TTTGAGATGT CCTACCGGCC CATGGACACA AGTTCTGAAC GTATCTCTGT CTTAAGCAGG
    CTTAGACAGA AAAAAGTGAT TACTTGGCTC CTGAACCATG ACCCAGCATC CCGCCCCACC
    GCTATGGAGT TGCTTAAGTG CGATCTCTTA CCCCCACCAC AACTAGAGGA GTCTGAACTC
    CATGAAGTCC TGCACCACAC CTTGGCCAAT ATCGATGGGA AAGCGTACCG CACAATGATG
    AGTCAAATCT TTGCCCAGCG CATTTCACCT GCTATGGATT ATACATATGA CAGTGATATA
    TTGAAGGGTA TTTTCTCAGT CCGTTCTGCC AGACTACATC ATCATGTTTG TGAGGCAGTC
    TGCAGGATAT TTAAAAGACA CGGGGCTGTG AAGTTACATG CCCCACTGCT GATGCCACGA
    GGTAAAGCTG GGTTTGATAA CAGTGAATCT GCATGTTTTA TGGATCACAG TGGCATGCTG
    GTCACCCTTC CTTATAACCT CAGGATATCA TTTGCGCGGT TTGTTGCAAG GAATAATATA
    ACTAGTTTGA AAAGGTATAG CATTGAGAGA GTGTTTAGAC CACGCAAACT GAACCGCTGC
    CATCCAAAGG AACTCACTGA GTGTGCATTT GATATAATCA CTTCTGCTGC AAACTGTTAC
    CTGCCCATTG CAGAAACAAT ATACACCATA TATGAAATTA TCCAGGAATT TCCAGTACTG
    CAGGAAAGGA ATTACAGTAT TTATGTGAAC CACACCTCGT TGTTGAAAGC CATTCTCCTT
    CACTGTGGCG TGCCCGAGGA TAAACTCACT CAGGTGTATA ACATTCTGTA TGATGCAATG
    ATTGAGAAGC TGACAAAAAG AGAAGTGGAA GCCAAATTTT GTAATCTCTC CCTTTCAGAG
    AACAGTTTCC AATTGAGTCG CCTTTACCGG TTTATTGAGC AGAAAGGAGA CCTCCCAGAT
    TTGGAGGCAA TGATGGTCCC ACTCACAAAG CAAAAAACAG GGGTCGGTGT GCTGGCAAAG
    CAAGGTTTGA AAGACTTGGA AGGAGTCATT GGATTAGTGA AGAGGCTGGG CATTAAATTA
    CAGATTGTGG TTAACTTTGG ACTTGTCTAC AAGATGCAGC AGCACAGTGG CATAGTCTTC
    CAGTTTGTGG CTTTTATCAA GCGGAGACAG AGGACTGTGC CTGAAATACT TGCAGCTGGA
    GGCAGATATG ACCATTTGAT TTCCCAGTTT AGGGGACCTC AGACTGGAGC TATGGGATTA
    CCCTGTGCTG TAGGCGTTAG TATAGCAATA GACAAGATAT CTGCTGCGCT TTCCACTGCA
    GAGGAACATA TCAGCAGCTG TGATTTATTA GTCGTAAGCG CTGGGCAGAT GTCTCTGAAT
    AGAGCCATTG GGATAACACA GAAGCTGTGG GCTGCTGGCA TTACTGCAGA GATCATGTAT
    GAGTGGTCTG AGTCTCAGGA TGAACTGCAG GATTATTGCA AGTGTTCGGG GATTTCGTAT
    GCTGCAATTA TTTCTGACAA AGAAGGAAAT CACATTAAGC TGAAAACATT TGAGAAGGAA
    AGACAGAGTG AGAAGAGAAT ATTGGAATCT GATCTAGTGG ATCTCCTGGT GCAGAAACTT
    CGGATCAGAG TCCTTGAAGA AAGAAATAAC AGAGAAACGT CGGACAGTCT CTCAGTGCAA
    AATGTGAAGG GCTCGTTTAA TTCCCAGAGT CAGTTTGAGC CACACAGCGC AATAATTGTG
    CCAAGTGTCA GTGTTATAGC ACCAGAAAAG CTATCTGCCA GTGCCAGGCG GAGATTTGAA
    ACCCAGGTGC TAACAAAGCT TCAGACACTC ATTACAAACT TCCAGCAGAG AAATGGTGAA
    ATTGAAATTT TAGCAGTTGA TCTGCCAAAA GAAACAATAC AGAACTTGTT AGCTTTAGAG
    TGCGCAGATG AACAAGTCTT CAATGCATCA GTGAAAATGC TAATGTCTCG GCTGCCAAAA
    CAGAGATACC TGAAATCAGT GTTTGATGAA ATATACACCA TTAAAATCGA AAAGCGGGTT
    CCTGTGCTGG TACTATACAG TTATAAAGAT GATTATTACA AGATCCTTTT TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017951667.1
    CDS648..5621
    Translation

    Target ORF information:

    RefSeq Version XM_018096178.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis eukaryotic translation initiation factor 2 alpha kinase 4 (eif2ak4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018096178.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCAGGCG GCCGGGGAAA TGCACTTCTT CCTATTGAGC TCAGCCCGCA AGGGGGTCTG 
    CGCCCTACTC CGCCTGCCTG TGACCCCCCT GAAACTTATT CCCACCGGCA GGAGAATGAG
    CTGGAGGTCC TGGAATCCAT CTATAGTGGG GATTTCGCGG ATCTGCGCAA GAACTCTGCC
    TGGAAGGTAC AGCAGCCTCC AGAAGTCATC ATTGCCTTAC GTCCTCAGGG AAATGAGGTT
    CATGTCAAGG TGGATTTATG GGTAAAATGC CCTCAGAACT ATCCTGATGT TCCTCCTGAA
    ATAAAGCTGA AGAACATCCA GGGGTTGTCC AATGAAAATG TGAACTCTCT GACATCAAGC
    TTGGAAGATT TAGCAAAACA GCTTTGTGGA GAGGTAATGA TATACCAGCT TGCGGATCAT
    GTGCAGTGTT TTCTTACAGA GTATAACCAT CCACCTCCTA AATCCTTTCA TGAGGAGATG
    TTAAAAAACC TTCAGAAGCA GCAGGAGAAG CTTGCTCTTG AAGAAGAGAG ACGAATGCAA
    GAGATAAAGA TGAAAGAAGA GCAGACGAGA CGGGAAATCC TTAATGAAAT CCAGAAGCGA
    GAAGAAGAGA AAAAGGAAGA AAAAAGAAGG AAGGAAGTAG CAAAACAGGA ACGGATTGAA
    AACGCTAATC TAATGTGCCA AGAGAATGTA AATAAGATTA ATAATACAGG CAAGACAACA
    ACAGCTGCCT CGGATGGGTC ACTTCCTGAT CATGGAGGAA CCAACAGAAA TCGGCCACAT
    TCAGCAGGAC GCTCAAAGCG GGACCGACAG TATTCGGTGT GCCTCACTGA TGACTCAACC
    CCATTTGAAG TGTTAACCTT TGATATTGGA TGCAGTGGGC AAATTGTTGT TCACAGAGGA
    AAGTGCCTGG GGAAAGATGA GCTGCTGGGT AAATCTGTTT ACAATGGCTT GGAAACTGCT
    TCAGGACATT TTGTTGTAAT CTATGAATGG GTTCTCCAGT GGCAGAAAAA GATGGGCAAG
    TTTCTTACTA ATCAAGAAAA AGAAAAGATT GAAAAGTGTA AAAAACAGAT TCAGGGAACA
    GAAACCGAGT TCAGTTCTCT TATCAAACTG AGCCATCCAA ACATTGTGCA CTACCTCGCT
    ATGCGAATAA AAGAGAAAGA AGATTCCATT TCTGTCGGGA TCATAGCTGA ACATGTTAAT
    GGTACAAAGC TTTCCAGTTA CCTTGAGCAG GGTGTACCTG TTCCACTGGA TCAACTACGG
    CACTATACTG CCCAGCTGCT ATCGGCACTG GACTATTTGC ATTCTAATTC TGTGGTACAT
    AAAGTCATGT GCGCCTCCAG TGTTTTGGTG ACAGCTGACG GGAAAGTAAA ACTGACAGAC
    TATAGCCTTT CCAAACGCCT TGCTGACATC TGCAAAGAGG ACGTGTTTGA GCAAACAAAA
    GTTCGATTCA GTGAGGATGC TCTGCCTAGC AAGAGTGGCA AGAAAGGAGA TATATGGAAG
    CTCGGTCTCT TGCTACTTTC GCTGTCACAA GGCAACATAG CCAAAGAGTA TCCTGTAAAA
    GTGCCCAGTG ACCTGCCTGC AGATTTCCAA GATTTCCTGC AAAAGTGTGT TTGCTTGGAA
    GACAAGGAAC GCTGGACTAC ACAGCAGTTA CTAAGTCACA GTTTCCTTAA CCCTCCACCT
    GAGAAGAGTC CTGTAACAGA GGAATGCTCA GATGATTATC CGGGTGACTG TACTGAGACT
    GTCATTCCCA GATCTTCAGT ATCTAATTCC ACATTCCATA TTGAGTCACA GAGACAGTTT
    TCTCGCTACT TCAATGAGTT TGAAGAATTA CAGATGCTTG GGAAAGGGGC ATTTGGTGCT
    GTCATTAAGG TTCAGAACAA ACTGGATGGC TGCTATTACG CTGTAAAACG GGTACAAATC
    AATCCTGCAA GTAAACAGTT CCGCAGAATT AAGGGGGAGG TAACATTGTT ATCTCGGCTC
    AACCATGAAA ACATTGTAAG GTACTATAAT GCATGGATTG AGAAACACGA GCATCCTACT
    ATTTCTCCTA AATGTGAAGC ACATGAGGAA AAGAAAGTGA CTGTGAAGCC AGAACAAATA
    GTAAAGAATG ATTTGGGATT GTTGGATGAT ATTGAGGAAA ACGCACCAGC ACCCATATTA
    ACCAGCTCTG TAGAATGGAG TACATCTTGC GAGCGATCAT CCAGTGCGCA CTGTACTAAT
    GGGGATCAAG ATTCAAGTGA TGATGATGAT GATGACGATG AAGAGGGAGT GTTTTCACCT
    TCGTTCCTGC CTGGGACAGA TTCTGACAGT GAGATCATAT TTGAGAATGA AGATGACATT
    AGTCCAGATC AGGATGAAGA ATATGACAAG AACAATGTAG AAGACCGCAA AGCTGGAGCC
    AATGAGATTG TGACTCAGGC AGTGCATTAT TTATATATTC AGATGGAATA TTGTGAGAAA
    AGCACCTTAC GGGACACGAT AGACCAAGGA CTGTATATGG ATGTTGGCCG CCTCTGGAGG
    CTGTTTCGTG AAATTTTGGA TGGATTGACC TATATACATG AGCAGGGAAT GATCCACAGA
    GACCTAAAGC CAGTCAATAT CTTTTTAGAT TCAGAAGATC ATGTCAAAAT AGGAGATTTT
    GGACTAGCTA CAGATCACCC AGCATCTTAT ACTGAATCAG CACAAGGGGA AGAGGATCAG
    GCGCATTCTG ACTTGGTTAA AGTCGACCCT GAAGATAGGG CCTCAACACC TGGTCGCAAT
    ACTCAAGGAA AACTCACTGG CATGGTTGGC ACTGCCTTGT ATGTAAGCCC TGAAGTCCAA
    GGAAACACCA AAGCTTCATA TAACCAGAAA GTGGACCTTT ACAGCCTGGG TATAATATTG
    TTTGAGATGT CCTACCGGCC CATGGACACA AGTTCTGAAC GTATCTCTGT CTTAAGCAGG
    CTTAGACAGA AAAAAGTGAT TACTTGGCTC CTGAACCATG ACCCAGCATC CCGCCCCACC
    GCTATGGAGT TGCTTAAGTG CGATCTCTTA CCCCCACCAC AACTAGAGGA GTCTGAACTC
    CATGAAGTCC TGCACCACAC CTTGGCCAAT ATCGATGGGA AAGCGTACCG CACAATGATG
    AGTCAAATCT TTGCCCAGCG CATTTCACCT GCTATGGATT ATACATATGA CAGTGATATA
    TTGAAGGGTA TTTTCTCAGT CCGTTCTGCC AGACTACATC ATCATGTTTG TGAGGCAGTC
    TGCAGGATAT TTAAAAGACA CGGGGCTGTG AAGTTACATG CCCCACTGCT GATGCCACGA
    GGTAAAGCTG GGTTTGATAA CAGTGAATCT GCATGTTTTA TGGATCACAG TGGCATGCTG
    GTCACCCTTC CTTATAACCT CAGGATATCA TTTGCGCGGT TTGTTGCAAG GAATAATATA
    ACTAGTTTGA AAAGGTATAG CATTGAGAGA GTGTTTAGAC CACGCAAACT GAACCGCTGC
    CATCCAAAGG AACTCACTGA GTGTGCATTT GATATAATCA CTTCTGCTGC AAACTGTTAC
    CTGCCCATTG CAGAAACAAT ATACACCATA TATGAAATTA TCCAGGAATT TCCAGTACTG
    CAGGAAAGGA ATTACAGTAT TTATGTGAAC CACACCTCGT TGTTGAAAGC CATTCTCCTT
    CACTGTGGCG TGCCCGAGGA TAAACTCACT CAGGTGTATA ACATTCTGTA TGATGCAATG
    ATTGAGAAGC TGACAAAAAG AGAAGTGGAA GCCAAATTTT GTAATCTCTC CCTTTCAGAG
    AACAGTTTCC AATTGAGTCG CCTTTACCGG TTTATTGAGC AGAAAGGAGA CCTCCCAGAT
    TTGGAGGCAA TGATGGTCCC ACTCACAAAG CAAAAAACAG GGGTCGGTGT GCTGGCAAAG
    CAAGGTTTGA AAGACTTGGA AGGAGTCATT GGATTAGTGA AGAGGCTGGG CATTAAATTA
    CAGATTGTGG TTAACTTTGG ACTTGTCTAC AAGATGCAGC AGCACAGTGG CATAGTCTTC
    CAGTTTGTGG CTTTTATCAA GCGGAGACAG AGGACTGTGC CTGAAATACT TGCAGCTGGA
    GGCAGATATG ACCATTTGAT TTCCCAGTTT AGGGGACCTC AGACTGGAGC TATGGGATTA
    CCCTGTGCTG TAGGCGTTAG TATAGCAATA GACAAGATAT CTGCTGCGCT TTCCACTGCA
    GAGGAACATA TCAGCAGCTG TGATTTATTA GTCGTAAGCG CTGGGCAGAT GTCTCTGAAT
    AGAGCCATTG GGATAACACA GAAGCTGTGG GCTGCTGGCA TTACTGCAGA GATCATGTAT
    GAGTGGTCTG AGTCTCAGGA TGAACTGCAG GATTATTGCA AGTGTTCGGG GATTTCGTAT
    GCTGCAATTA TTTCTGACAA AGAAGGAAAT CACATTAAGC TGAAAACATT TGAGAAGGAA
    AGACAGAGTG AGAAGAGAAT ATTGGAATCT GATCTAGTGG ATCTCCTGGT GCAGAAACTT
    CGGATCAGAG TCCTTGAAGA AAGAAATAAC AGAGAAACGT CGGACAGTCT CTCAGTGCAA
    AATGTGAAGG GCTCGTTTAA TTCCCAGAGT CAGTTTGAGC CACACAGCGC AATAATTGTG
    CCAAGTGTCA GTGTTATAGC ACCAGAAAAG CTATCTGCCA GTGCCAGGCG GAGATTTGAA
    ACCCAGGTGC TAACAAAGCT TCAGACACTC ATTACAAACT TCCAGCAGAG AAATGGTGAA
    ATTGAAATTT TAGCAGTTGA TCTGCCAAAA GAAACAATAC AGAACTTGTT AGCTTTAGAG
    TGCGCAGATG AACAAGTCTT CAATGCATCA GTGAAAATGC TAATGTCTCG GCTGCCAAAA
    CAGAGATACC TGAAATCAGT GTTTGATGAA ATATACACCA TTAAAATCGA AAAGCGGGTT
    CCTGTGCTGG TACTATACAG TTATAAAGAT GATTATTACA AGATCCTTTT TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa20310
    Clone ID Related Accession (Same CDS sequence) NM_001256926.1
    Accession Version NM_001256926.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5007bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Xenopus tropicalis(tropical clawed frog)
    Product eIF-2-alpha kinase GCN2
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAMC03032456.1, AAMC03032457.1 and CX847035.1. On Mar 9, 2012 this sequence version replaced XM_002936897.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00861995, SAMN01758126 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGCAGGCG GCCGGGGAAA TGCACTTCTT CCTATTGAGC TCAGCCCGCA AGGGGGTCTG 
    CGCCCTACTC CGCCTGCCTG TGACCCCCCT GAAACTTATT CCCACCGGCA GGAGAATGAG
    CTGGAGGTCC TGGAATCCAT CTATAGTGGG GATTTCGCGG ATCTGCGCAA GAACTCTGCC
    TGGAAGGTAC AGCAGCCTCC AGAAGTCATC ATTGCCTTAC GTCCTCAGGG AAATGAGGTT
    CATGTCAAGG TGGATTTATG GGTAAAATGC CCTCAGAACT ATCCTGATGT TCCTCCTGAA
    ATAAAGCTGA AGAACATCCA GGGGTTGTCC AATGAAAATG TGAACTCTCT GACATCAAGC
    TTGGAAGATT TAGCAAAACA GCTTTGTGGA GAGGTAATGA TATACCAGCT TGCGGATCAT
    GTGCAGTGTT TTCTTACAGA GTATAACCAT CCACCTCCTA AATCCTTTCA TGAGGAGATG
    TTAAAAAACC TTCAGAAGCA GCAGGAGAAG CTTGCTCTTG AAGAAGAGAG ACGAATGCAA
    GAGATAAAGA TGAAAGAAGA GCAGACGAGA CGGGAAATCC TTAATGAAAT CCAGAAGCGA
    GAAGAAGAGA AAAAGGAAGA AAAAAGAAGG AAGGAAGTAG CAAAACAGGA ACGGATTGAA
    AACGCTAATC TAATGTGCCA AGAGAATGTA AATAAGATTA ATAATACAGG CAAGACAACA
    ACAGCTGCCT CGGATGGGTC ACTTCCTGAT CATGGAGGAA CCAACAGAAA TCGGCCACAT
    TCAGCAGGAC GCTCAAAGCG GGACCGACAG TATTCGGTGT GCCTCACTGA TGACTCAACC
    CCATTTGAAG TGTTAACCTT TGATATTGGA TGCAGTGGGC AAATTGTTGT TCACAGAGGA
    AAGTGCCTGG GGAAAGATGA GCTGCTGGGT AAATCTGTTT ACAATGGCTT GGAAACTGCT
    TCAGGACATT TTGTTGTAAT CTATGAATGG GTTCTCCAGT GGCAGAAAAA GATGGGCAAG
    TTTCTTACTA ATCAAGAAAA AGAAAAGATT GAAAAGTGTA AAAAACAGAT TCAGGGAACA
    GAAACCGAGT TCAGTTCTCT TATCAAACTG AGCCATCCAA ACATTGTGCA CTACCTCGCT
    ATGCGAATAA AAGAGAAAGA AGATTCCATT TCTGTCGGGA TCATAGCTGA ACATGTTAAT
    GGTACAAAGC TTTCCAGTTA CCTTGAGCAG GGTGTACCTG TTCCACTGGA TCAACTACGG
    CACTATACTG CCCAGCTGCT ATCGGCACTG GACTATTTGC ATTCTAATTC TGTGGTACAT
    AAAGTCATGT GCGCCTCCAG TGTTTTGGTG ACAGCTGACG GGAAAGTAAA ACTGACAGAC
    TATAGCCTTT CCAAACGCCT TGCTGACATC TGCAAAGAGG ACGTGTTTGA GCAAACAAAA
    GTTCGATTCA GTGAGGATGC TCTGCCTAGC AAGAGTGGCA AGAAAGGAGA TATATGGAAG
    CTCGGTCTCT TGCTACTTTC GCTGTCACAA GGCAACATAG CCAAAGAGTA TCCTGTAAAA
    GTGCCCAGTG ACCTGCCTGC AGATTTCCAA GATTTCCTGC AAAAGTGTGT TTGCTTGGAA
    GACAAGGAAC GCTGGACTAC ACAGCAGTTA CTAAGTCACA GTTTCCTTAA CCCTCCACCT
    GAGAAGAGTC CTGTAACAGA GGAATGCTCA GATGATTATC CGGGTGACTG TACTGAGACT
    GTCATTCCCA GATCTTCAGT ATCTAATTCC ACATTCCATA TTGAGTCACA GAGACAGTTT
    TCTCGCTACT TCAATGAGTT TGAAGAATTA CAGATGCTTG GGAAAGGGGC ATTTGGTGCT
    GTCATTAAGG TTCAGAACAA ACTGGATGGC TGCTATTACG CTGTAAAACG GGTACAAATC
    AATCCTGCAA GTAAACAGTT CCGCAGAATT AAGGGGGAGG TAACATTGTT ATCTCGGCTC
    AACCATGAAA ACATTGTAAG GTACTATAAT GCATGGATTG AGAAACACGA GCATCCTACT
    ATTTCTCCTA AATGTGAAGC ACATGAGGAA AAGAAAGTGA CTGTGAAGCC AGAACAAATA
    GTAAAGAATG ATTTGGGATT GTTGGATGAT ATTGAGGAAA ACGCACCAGC ACCCATATTA
    ACCAGCTCTG TAGAATGGAG TACATCTTGC GAGCGATCAT CCAGTGCGCA CTGTACTAAT
    GGGGATCAAG ATTCAAGTGA TGATGATGAT GATGACGATG AAGAGGGAGT GTTTTCACCT
    TCGTTCCTGC CTGGGACAGA TTCTGACAGT GAGATCATAT TTGAGAATGA AGATGACATT
    AGTCCAGATC AGAACAATGT AGAAGACCGC AAAGCTGGAG CCAATGAGAT TGTGACTCAG
    GCAGTGCATT ATTTATATAT TCAGATGGAA TATTGTGAGA AAAGCACCTT ACGGGACACG
    ATAGACCAAG GACTGTATAT GGATGTTGGC CGCCTCTGGA GGCTGTTTCG TGAAATTTTG
    GATGGATTGA CCTATATACA TGAGCAGGGA ATGATCCACA GAGACCTAAA GCCAGTCAAT
    ATCTTTTTAG ATTCAGAAGA TCATGTCAAA ATAGGAGATT TTGGACTAGC TACAGATCAC
    CCAGCATCTT ATACTGAATC AGCACAAGGG GAAGAGGATC AGGCGCATTC TGACTTGGTT
    AAAGTCGACC CTGAAGATAG GGCCTCAACA CCTGGTCGCA ATACTCAAGG AAAACTCACT
    GGCATGGTTG GCACTGCCTT GTATGTAAGC CCTGAAGTCC AAGGAAACAC CAAAGCTTCA
    TATAACCAGA AAGTGGACCT TTACAGCCTG GGTATAATAT TGTTTGAGAT GTCCTACCGG
    CCCATGGACA CAAGTTCTGA ACGTATCTCT GTCTTAAGCA GGCTTAGACA GGCCTTGATT
    GTGTTCCCAA GTGATTTTGA AAACCCAGAA AATGAAAAAC AGAAAAAAGT GATTACTTGG
    CTCCTGAACC ATGACCCAGC ATCCCGCCCC ACCGCTATGG AGTTGCTTAA GTGCGATCTC
    TTACCCCCAC CACAACTAGA GGAGTCTGAA CTCCATGAAG TCCTGCACCA CACCTTGGCC
    AATATCGATG GGAAAGCGTA CCGCACAATG ATGAGTCAAA TCTTTGCCCA GCGCATTTCA
    CCTGCTATGG ATTATACATA TGACAGTGAT ATATTGAAGG GTATTTTCTC AGTCCGTTCT
    GCCAGACTAC ATCATCATGT TTGTGAGGCA GTCTGCAGGA TATTTAAAAG ACACGGGGCT
    GTGAAGTTAC ATGCCCCACT GCTGATGCCA CGAGGTAAAG CTGGGTTTGA TAACAGTGAA
    TCTGCATGTT TTATGGATCA CAGTGGCATG CTGGTCACCC TTCCTTATAA CCTCAGGATA
    TCATTTGCGC GGTTTGTTGC AAGGAATAAT ATAACTAGTT TGAAAAGGTA TAGCATTGAG
    AGAGTGTTTA GACCACGCAA ACTGAACCGC TGCCATCCAA AGGAACTCAC TGAGTGTGCA
    TTTGATATAA TCACTTCTGC TGCAAACTGT TACCTGCCCA TTGCAGAAAC AATATACACC
    ATATATGAAA TTATCCAGGA ATTTCCAGTA CTGCAGGAAA GGAATTACAG TATTTATGTG
    AACCACACCT CGTTGTTGAA AGCCATTCTC CTTCACTGTG GCGTGCCCGA GGATAAACTC
    ACTCAGGTGT ATAACATTCT GTATGATGCA ATGATTGAGA AGCTGACAAA AAGAGAAGTG
    GAAGCCAAAT TTTGTAATCT CTCCCTTTCA GAGAACAGTT TCCAATTGAG TCGCCTTTAC
    CGGTTTATTG AGCAGAAAGG AGACCTCCCA GATTTGGAGG CAATGATGGT CCCACTCACA
    AAGCAAAAAA CAGGGGTCGG TGTGCTGGCA AAGCAAGGTT TGAAAGACTT GGAAGGAGTC
    ATTGGATTAG TGAAGAGGCT GGGCATTAAA TTACAGATTG TGGTTAACTT TGGACTTGTC
    TACAAGATGC AGCAGCACAG TGGCATAGTC TTCCAGTTTG TGGCTTTTAT CAAGCGGAGA
    CAGAGGACTG TGCCTGAAAT ACTTGCAGCT GGAGGCAGAT ATGACCATTT GATTTCCCAG
    TTTAGGGGAC CTCAGACTGG AGCTATGGGA TTACCCTGTG CTGTAGGCGT TAGTATAGCA
    ATAGACAAGA TATCTGCTGC GCTTTCCACT GCAGAGGAAC ATATCAGCAG CTGTGATTTA
    TTAGTCGTAA GCGCTGGGCA GATGTCTCTG AATAGAGCCA TTGGGATAAC ACAGAAGCTG
    TGGGCTGCTG GCATTACTGC AGAGATCATG TATGAGTGGT CTGAGTCTCA GGATGAACTG
    CAGGATTATT GCAAGTGTTC GGGGATTTCG TATGCTGCAA TTATTTCTGA CAAAGAAGGA
    AATCACATTA AGCTGAAAAC ATTTGAGAAG GAAAGACAGA GTGAGAAGAG AATATTGGAA
    TCTGATCTAG TGGATCTCCT GGTGCAGAAA CTTCGGATCA GAGTCCTTGA AGAAAGAAAT
    AACAGAGAAA CGTCGGACAG TCTCTCAGTG CAAAATGTGA AGGGCTCGTT TAATTCCCAG
    AGTCAGTTTG AGCCACACAG CGCAATAATT GTGCCAAGTG TCAGTGTTAT AGCACCAGAA
    AAGCTATCTG CCAGTGCCAG GCGGAGATTT GAAACCCAGG TGCTAACAAA GCTTCAGACA
    CTCATTACAA ACTTCCAGCA GAGAAATGGT GAAATTGAAA TTTTAGCAGT TGATCTGCCA
    AAAGAAACAA TACAGAACTT GTTAGCTTTA GAGTGCGCAG ATGAACAAGT CTTCAATGCA
    TCAGTGAAAA TGCTAATGTC TCGGCTGCCA AAACAGAGAT ACCTGAAATC AGTGTTTGAT
    GAAATATACA CCATTAAAAT CGAAAAGCGG GTTCCTGTGC TGGTACTATA CAGTTATAAA
    GATGATTATT ACAAGATCCT TTTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001243855.1
    CDS91..5097
    Translation

    Target ORF information:

    RefSeq Version NM_001256926.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis eukaryotic translation initiation factor 2 alpha kinase 4 (eif2ak4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001256926.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGCAGGCG GCCGGGGAAA TGCACTTCTT CCTATTGAGC TCAGCCCGCA AGGGGGTCTG 
    CGCCCTACTC CGCCTGCCTG TGACCCCCCT GAAACTTATT CCCACCGGCA GGAGAATGAG
    CTGGAGGTCC TGGAATCCAT CTATAGTGGG GATTTCGCGG ATCTGCGCAA GAACTCTGCC
    TGGAAGGTAC AGCAGCCTCC AGAAGTCATC ATTGCCTTAC GTCCTCAGGG AAATGAGGTT
    CATGTCAAGG TGGATTTATG GGTAAAATGC CCTCAGAACT ATCCTGATGT TCCTCCTGAA
    ATAAAGCTGA AGAACATCCA GGGGTTGTCC AATGAAAATG TGAACTCTCT GACATCAAGC
    TTGGAAGATT TAGCAAAACA GCTTTGTGGA GAGGTAATGA TATACCAGCT TGCGGATCAT
    GTGCAGTGTT TTCTTACAGA GTATAACCAT CCACCTCCTA AATCCTTTCA TGAGGAGATG
    TTAAAAAACC TTCAGAAGCA GCAGGAGAAG CTTGCTCTTG AAGAAGAGAG ACGAATGCAA
    GAGATAAAGA TGAAAGAAGA GCAGACGAGA CGGGAAATCC TTAATGAAAT CCAGAAGCGA
    GAAGAAGAGA AAAAGGAAGA AAAAAGAAGG AAGGAAGTAG CAAAACAGGA ACGGATTGAA
    AACGCTAATC TAATGTGCCA AGAGAATGTA AATAAGATTA ATAATACAGG CAAGACAACA
    ACAGCTGCCT CGGATGGGTC ACTTCCTGAT CATGGAGGAA CCAACAGAAA TCGGCCACAT
    TCAGCAGGAC GCTCAAAGCG GGACCGACAG TATTCGGTGT GCCTCACTGA TGACTCAACC
    CCATTTGAAG TGTTAACCTT TGATATTGGA TGCAGTGGGC AAATTGTTGT TCACAGAGGA
    AAGTGCCTGG GGAAAGATGA GCTGCTGGGT AAATCTGTTT ACAATGGCTT GGAAACTGCT
    TCAGGACATT TTGTTGTAAT CTATGAATGG GTTCTCCAGT GGCAGAAAAA GATGGGCAAG
    TTTCTTACTA ATCAAGAAAA AGAAAAGATT GAAAAGTGTA AAAAACAGAT TCAGGGAACA
    GAAACCGAGT TCAGTTCTCT TATCAAACTG AGCCATCCAA ACATTGTGCA CTACCTCGCT
    ATGCGAATAA AAGAGAAAGA AGATTCCATT TCTGTCGGGA TCATAGCTGA ACATGTTAAT
    GGTACAAAGC TTTCCAGTTA CCTTGAGCAG GGTGTACCTG TTCCACTGGA TCAACTACGG
    CACTATACTG CCCAGCTGCT ATCGGCACTG GACTATTTGC ATTCTAATTC TGTGGTACAT
    AAAGTCATGT GCGCCTCCAG TGTTTTGGTG ACAGCTGACG GGAAAGTAAA ACTGACAGAC
    TATAGCCTTT CCAAACGCCT TGCTGACATC TGCAAAGAGG ACGTGTTTGA GCAAACAAAA
    GTTCGATTCA GTGAGGATGC TCTGCCTAGC AAGAGTGGCA AGAAAGGAGA TATATGGAAG
    CTCGGTCTCT TGCTACTTTC GCTGTCACAA GGCAACATAG CCAAAGAGTA TCCTGTAAAA
    GTGCCCAGTG ACCTGCCTGC AGATTTCCAA GATTTCCTGC AAAAGTGTGT TTGCTTGGAA
    GACAAGGAAC GCTGGACTAC ACAGCAGTTA CTAAGTCACA GTTTCCTTAA CCCTCCACCT
    GAGAAGAGTC CTGTAACAGA GGAATGCTCA GATGATTATC CGGGTGACTG TACTGAGACT
    GTCATTCCCA GATCTTCAGT ATCTAATTCC ACATTCCATA TTGAGTCACA GAGACAGTTT
    TCTCGCTACT TCAATGAGTT TGAAGAATTA CAGATGCTTG GGAAAGGGGC ATTTGGTGCT
    GTCATTAAGG TTCAGAACAA ACTGGATGGC TGCTATTACG CTGTAAAACG GGTACAAATC
    AATCCTGCAA GTAAACAGTT CCGCAGAATT AAGGGGGAGG TAACATTGTT ATCTCGGCTC
    AACCATGAAA ACATTGTAAG GTACTATAAT GCATGGATTG AGAAACACGA GCATCCTACT
    ATTTCTCCTA AATGTGAAGC ACATGAGGAA AAGAAAGTGA CTGTGAAGCC AGAACAAATA
    GTAAAGAATG ATTTGGGATT GTTGGATGAT ATTGAGGAAA ACGCACCAGC ACCCATATTA
    ACCAGCTCTG TAGAATGGAG TACATCTTGC GAGCGATCAT CCAGTGCGCA CTGTACTAAT
    GGGGATCAAG ATTCAAGTGA TGATGATGAT GATGACGATG AAGAGGGAGT GTTTTCACCT
    TCGTTCCTGC CTGGGACAGA TTCTGACAGT GAGATCATAT TTGAGAATGA AGATGACATT
    AGTCCAGATC AGAACAATGT AGAAGACCGC AAAGCTGGAG CCAATGAGAT TGTGACTCAG
    GCAGTGCATT ATTTATATAT TCAGATGGAA TATTGTGAGA AAAGCACCTT ACGGGACACG
    ATAGACCAAG GACTGTATAT GGATGTTGGC CGCCTCTGGA GGCTGTTTCG TGAAATTTTG
    GATGGATTGA CCTATATACA TGAGCAGGGA ATGATCCACA GAGACCTAAA GCCAGTCAAT
    ATCTTTTTAG ATTCAGAAGA TCATGTCAAA ATAGGAGATT TTGGACTAGC TACAGATCAC
    CCAGCATCTT ATACTGAATC AGCACAAGGG GAAGAGGATC AGGCGCATTC TGACTTGGTT
    AAAGTCGACC CTGAAGATAG GGCCTCAACA CCTGGTCGCA ATACTCAAGG AAAACTCACT
    GGCATGGTTG GCACTGCCTT GTATGTAAGC CCTGAAGTCC AAGGAAACAC CAAAGCTTCA
    TATAACCAGA AAGTGGACCT TTACAGCCTG GGTATAATAT TGTTTGAGAT GTCCTACCGG
    CCCATGGACA CAAGTTCTGA ACGTATCTCT GTCTTAAGCA GGCTTAGACA GGCCTTGATT
    GTGTTCCCAA GTGATTTTGA AAACCCAGAA AATGAAAAAC AGAAAAAAGT GATTACTTGG
    CTCCTGAACC ATGACCCAGC ATCCCGCCCC ACCGCTATGG AGTTGCTTAA GTGCGATCTC
    TTACCCCCAC CACAACTAGA GGAGTCTGAA CTCCATGAAG TCCTGCACCA CACCTTGGCC
    AATATCGATG GGAAAGCGTA CCGCACAATG ATGAGTCAAA TCTTTGCCCA GCGCATTTCA
    CCTGCTATGG ATTATACATA TGACAGTGAT ATATTGAAGG GTATTTTCTC AGTCCGTTCT
    GCCAGACTAC ATCATCATGT TTGTGAGGCA GTCTGCAGGA TATTTAAAAG ACACGGGGCT
    GTGAAGTTAC ATGCCCCACT GCTGATGCCA CGAGGTAAAG CTGGGTTTGA TAACAGTGAA
    TCTGCATGTT TTATGGATCA CAGTGGCATG CTGGTCACCC TTCCTTATAA CCTCAGGATA
    TCATTTGCGC GGTTTGTTGC AAGGAATAAT ATAACTAGTT TGAAAAGGTA TAGCATTGAG
    AGAGTGTTTA GACCACGCAA ACTGAACCGC TGCCATCCAA AGGAACTCAC TGAGTGTGCA
    TTTGATATAA TCACTTCTGC TGCAAACTGT TACCTGCCCA TTGCAGAAAC AATATACACC
    ATATATGAAA TTATCCAGGA ATTTCCAGTA CTGCAGGAAA GGAATTACAG TATTTATGTG
    AACCACACCT CGTTGTTGAA AGCCATTCTC CTTCACTGTG GCGTGCCCGA GGATAAACTC
    ACTCAGGTGT ATAACATTCT GTATGATGCA ATGATTGAGA AGCTGACAAA AAGAGAAGTG
    GAAGCCAAAT TTTGTAATCT CTCCCTTTCA GAGAACAGTT TCCAATTGAG TCGCCTTTAC
    CGGTTTATTG AGCAGAAAGG AGACCTCCCA GATTTGGAGG CAATGATGGT CCCACTCACA
    AAGCAAAAAA CAGGGGTCGG TGTGCTGGCA AAGCAAGGTT TGAAAGACTT GGAAGGAGTC
    ATTGGATTAG TGAAGAGGCT GGGCATTAAA TTACAGATTG TGGTTAACTT TGGACTTGTC
    TACAAGATGC AGCAGCACAG TGGCATAGTC TTCCAGTTTG TGGCTTTTAT CAAGCGGAGA
    CAGAGGACTG TGCCTGAAAT ACTTGCAGCT GGAGGCAGAT ATGACCATTT GATTTCCCAG
    TTTAGGGGAC CTCAGACTGG AGCTATGGGA TTACCCTGTG CTGTAGGCGT TAGTATAGCA
    ATAGACAAGA TATCTGCTGC GCTTTCCACT GCAGAGGAAC ATATCAGCAG CTGTGATTTA
    TTAGTCGTAA GCGCTGGGCA GATGTCTCTG AATAGAGCCA TTGGGATAAC ACAGAAGCTG
    TGGGCTGCTG GCATTACTGC AGAGATCATG TATGAGTGGT CTGAGTCTCA GGATGAACTG
    CAGGATTATT GCAAGTGTTC GGGGATTTCG TATGCTGCAA TTATTTCTGA CAAAGAAGGA
    AATCACATTA AGCTGAAAAC ATTTGAGAAG GAAAGACAGA GTGAGAAGAG AATATTGGAA
    TCTGATCTAG TGGATCTCCT GGTGCAGAAA CTTCGGATCA GAGTCCTTGA AGAAAGAAAT
    AACAGAGAAA CGTCGGACAG TCTCTCAGTG CAAAATGTGA AGGGCTCGTT TAATTCCCAG
    AGTCAGTTTG AGCCACACAG CGCAATAATT GTGCCAAGTG TCAGTGTTAT AGCACCAGAA
    AAGCTATCTG CCAGTGCCAG GCGGAGATTT GAAACCCAGG TGCTAACAAA GCTTCAGACA
    CTCATTACAA ACTTCCAGCA GAGAAATGGT GAAATTGAAA TTTTAGCAGT TGATCTGCCA
    AAAGAAACAA TACAGAACTT GTTAGCTTTA GAGTGCGCAG ATGAACAAGT CTTCAATGCA
    TCAGTGAAAA TGCTAATGTC TCGGCTGCCA AAACAGAGAT ACCTGAAATC AGTGTTTGAT
    GAAATATACA CCATTAAAAT CGAAAAGCGG GTTCCTGTGC TGGTACTATA CAGTTATAAA
    GATGATTATT ACAAGATCCT TTTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32794
    Clone ID Related Accession (Same CDS sequence) XM_012968127.2 , XM_012968127.3
    Accession Version XM_012968127.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5025bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product eIF-2-alpha kinase GCN2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968127.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGCAGGCG GCCGGGGAAA TGCACTTCTT CCTATTGAGC TCAGCCCGCA AGGGGGTCTG 
    CGCCCTACTC CGCCTGCCTG TGACCCCCCT GAAACTTATT CCCACCGGCA GGAGAATGAG
    CTGGAGGTCC TGGAATCCAT CTATAGTGGG GATTTCGCGG ATCTGCGCAA GAACTCTGCC
    TGGAAGGTAC AGCAGCCTCC AGAAGTCATC ATTGCCTTAC GTCCTCAGGG AAATGAGGTT
    CATGTCAAGG TGGATTTATG GGTAAAATGC CCTCAGAACT ATCCTGATGT TCCTCCTGAA
    ATAAAGCTGA AGAACATCCA GGGGTTGTCC AATGAAAATG TGAACTCTCT GACATCAAGC
    TTGGAAGATT TAGCAAAACA GCTTTGTGGA GAGGTAATGA TATACCAGCT TGCGGATCAT
    GTGCAGTGTT TTCTTACAGA GTATAACCAT CCACCTCCTA AATCCTTTCA TGAGGAGATG
    TTAAAAAACC TTCAGAAGCA GCAGGAGAAG CTTGCTCTTG AAGAAGAGAG ACGAATGCAA
    GAGATAAAGA TGAAAGAAGA GCAGACGAGA CGGGAAATCC TTAATGAAAT CCAGAAGCGA
    GAAGAAGAGA AAAAGGAAGA AAAAAGAAGG AAGGAAGTAG CAAAACAGGA ACGGATTGAA
    AACGCTAATC TAATGTGCCA AGAGAATGTA AATAAGATTA ATAATACAGG CAAGACAACA
    ACAGCTGCCT CGGATGGGTC ACTTCCTGAT CATGGAGGAA CCAACAGAAA TCGGCCACAT
    TCAGCAGGAC GCTCAAAGCG GGACCGACAG TATTCGGTGT GCCTCACTGA TGACTCAACC
    CCATTTGAAG TGTTAACCTT TGATATTGGA TGCAGTGGGC AAATTGTTGT TCACAGAGGA
    AAGTGCCTGG GGAAAGATGA GCTGCTGGGT AAATCTGTTT ACAATGGCTT GGAAACTGCT
    TCAGGACATT TTGTTGTAAT CTATGAATGG GTTCTCCAGT GGCAGAAAAA GATGGGCAAG
    TTTCTTACTA ATCAAGAAAA AGAAAAGATT GAAAAGTGTA AAAAACAGAT TCAGGGAACA
    GAAACCGAGT TCAGTTCTCT TATCAAACTG AGCCATCCAA ACATTGTGCA CTACCTCGCT
    ATGCGAATAA AAGAGAAAGA AGATTCCATT TCTGTCGGGA TCATAGCTGA ACATGTTAAT
    GGTACAAAGC TTTCCAGTTA CCTTGAGCAG GGTGTACCTG TTCCACTGGA TCAACTACGG
    CACTATACTG CCCAGCTGCT ATCGGCACTG GACTATTTGC ATTCTAATTC TGTGGTACAT
    AAAGTCATGT GCGCCTCCAG TGTTTTGGTG ACAGCTGACG GGAAAGTAAA ACTGACAGAC
    TATAGCCTTT CCAAACGCCT TGCTGACATC TGCAAAGAGG ACGTGTTTGA GCAAACAAAA
    GTTCGATTCA GTGAGGATGC TCTGCCTAGC AAGAGTGGCA AGAAAGGAGA TATATGGAAG
    CTCGGTCTCT TGCTACTTTC GCTGTCACAA GGCAACATAG CCAAAGAGTA TCCTGTAAAA
    GTGCCCAGTG ACCTGCCTGC AGATTTCCAA GATTTCCTGC AAAAGTGTGT TTGCTTGGAA
    GACAAGGAAC GCTGGACTAC ACAGCAGTTA CTAAGTCACA GTTTCCTTAA CCCTCCACCT
    GAGAAGAGTC CTGTAACAGA GGAATGCTCA GATGATTATC CGGGTGACTG TACTGAGACT
    GTCATTCCCA GATCTTCAGT ATCTAATTCC ACATTCCATA TTGAGTCACA GAGACAGTTT
    TCTCGCTACT TCAATGAGTT TGAAGAATTA CAGATGCTTG GGAAAGGGGC ATTTGGTGCT
    GTCATTAAGG TTCAGAACAA ACTGGATGGC TGCTATTACG CTGTAAAACG GGTACAAATC
    AATCCTGCAA GTAAACAGTT CCGCAGAATT AAGGGGGAGG TAACATTGTT ATCTCGGCTC
    AACCATGAAA ACATTGTAAG GTACTATAAT GCATGGATTG AGAAACACGA GCATCCTACT
    ATTTCTCCTA AATGTGAAGC ACATGAGGAA AAGAAAGTGA CTGTGAAGCC AGAACAAATA
    GTAAAGAATG ATTTGGGATT GTTGGATGAT ATTGAGGAAA ACGCACCAGC ACCCATATTA
    ACCAGCTCTG TAGAATGGAG TACATCTTGC GAGCGATCAT CCAGTGCGCA CTGTACTAAT
    GGGGATCAAG ATTCAAGTGA TGATGATGAT GATGACGATG AAGAGGGAGT GTTTTCACCT
    TCGTTCCTGC CTGGGACAGA TTCTGACAGT GAGATCATAT TTGAGAATGA AGATGACATT
    AGTCCAGATC AGGATGAAGA ATATGACAAG AACAATGTAG AAGACCGCAA AGCTGGAGCC
    AATGAGATTG TGACTCAGGC AGTGCATTAT TTATATATTC AGATGGAATA TTGTGAGAAA
    AGCACCTTAC GGGACACGAT AGACCAAGGA CTGTATATGG ATGTTGGCCG CCTCTGGAGG
    CTGTTTCGTG AAATTTTGGA TGGATTGACC TATATACATG AGCAGGGAAT GATCCACAGA
    GACCTAAAGC CAGTCAATAT CTTTTTAGAT TCAGAAGATC ATGTCAAAAT AGGAGATTTT
    GGACTAGCTA CAGATCACCC AGCATCTTAT ACTGAATCAG CACAAGGGGA AGAGGATCAG
    GCGCATTCTG ACTTGGTTAA AGTCGACCCT GAAGATAGGG CCTCAACACC TGGTCGCAAT
    ACTCAAGGAA AACTCACTGG CATGGTTGGC ACTGCCTTGT ATGTAAGCCC TGAAGTCCAA
    GGAAACACCA AAGCTTCATA TAACCAGAAA GTGGACCTTT ACAGCCTGGG TATAATATTG
    TTTGAGATGT CCTACCGGCC CATGGACACA AGTTCTGAAC GTATCTCTGT CTTAAGCAGG
    CTTAGACAGG CCTTGATTGT GTTCCCAAGT GATTTTGAAA ACCCAGAAAA TGAAAAACAG
    AAAAAAGTGA TTACTTGGCT CCTGAACCAT GACCCAGCAT CCCGCCCCAC CGCTATGGAG
    TTGCTTAAGT GCGATCTCTT ACCCCCACCA CAACTAGAGG AGTCTGAACT CCATGAAGTC
    CTGCACCACA CCTTGGCCAA TATCGATGGG AAAGCGTACC GCACAATGAT GAGTCAAATC
    TTTGCCCAGC GCATTTCACC TGCTATGGAT TATACATATG ACAGTGATAT ATTGAAGGGT
    ATTTTCTCAG TCCGTTCTGC CAGACTACAT CATCATGTTT GTGAGGCAGT CTGCAGGATA
    TTTAAAAGAC ACGGGGCTGT GAAGTTACAT GCCCCACTGC TGATGCCACG AGGTAAAGCT
    GGGTTTGATA ACAGTGAATC TGCATGTTTT ATGGATCACA GTGGCATGCT GGTCACCCTT
    CCTTATAACC TCAGGATATC ATTTGCGCGG TTTGTTGCAA GGAATAATAT AACTAGTTTG
    AAAAGGTATA GCATTGAGAG AGTGTTTAGA CCACGCAAAC TGAACCGCTG CCATCCAAAG
    GAACTCACTG AGTGTGCATT TGATATAATC ACTTCTGCTG CAAACTGTTA CCTGCCCATT
    GCAGAAACAA TATACACCAT ATATGAAATT ATCCAGGAAT TTCCAGTACT GCAGGAAAGG
    AATTACAGTA TTTATGTGAA CCACACCTCG TTGTTGAAAG CCATTCTCCT TCACTGTGGC
    GTGCCCGAGG ATAAACTCAC TCAGGTGTAT AACATTCTGT ATGATGCAAT GATTGAGAAG
    CTGACAAAAA GAGAAGTGGA AGCCAAATTT TGTAATCTCT CCCTTTCAGA GAACAGTTTC
    CAATTGAGTC GCCTTTACCG GTTTATTGAG CAGAAAGGAG ACCTCCCAGA TTTGGAGGCA
    ATGATGGTCC CACTCACAAA GCAAAAAACA GGGGTCGGTG TGCTGGCAAA GCAAGGTTTG
    AAAGACTTGG AAGGAGTCAT TGGATTAGTG AAGAGGCTGG GCATTAAATT ACAGATTGTG
    GTTAACTTTG GACTTGTCTA CAAGATGCAG CAGCACAGTG GCATAGTCTT CCAGTTTGTG
    GCTTTTATCA AGCGGAGACA GAGGACTGTG CCTGAAATAC TTGCAGCTGG AGGCAGATAT
    GACCATTTGA TTTCCCAGTT TAGGGGACCT CAGACTGGAG CTATGGGATT ACCCTGTGCT
    GTAGGCGTTA GTATAGCAAT AGACAAGATA TCTGCTGCGC TTTCCACTGC AGAGGAACAT
    ATCAGCAGCT GTGATTTATT AGTCGTAAGC GCTGGGCAGA TGTCTCTGAA TAGAGCCATT
    GGGATAACAC AGAAGCTGTG GGCTGCTGGC ATTACTGCAG AGATCATGTA TGAGTGGTCT
    GAGTCTCAGG ATGAACTGCA GGATTATTGC AAGTGTTCGG GGATTTCGTA TGCTGCAATT
    ATTTCTGACA AAGAAGGAAA TCACATTAAG CTGAAAACAT TTGAGAAGGA AAGACAGAGT
    GAGAAGAGAA TATTGGAATC TGATCTAGTG GATCTCCTGG TGCAGAAACT TCGGATCAGA
    GTCCTTGAAG AAAGAAATAA CAGAGAAACG TCGGACAGTC TCTCAGTGCA AAATGTGAAG
    GGCTCGTTTA ATTCCCAGAG TCAGTTTGAG CCACACAGCG CAATAATTGT GCCAAGTGTC
    AGTGTTATAG CACCAGAAAA GCTATCTGCC AGTGCCAGGC GGAGATTTGA AACCCAGGTG
    CTAACAAAGC TTCAGACACT CATTACAAAC TTCCAGCAGA GAAATGGTGA AATTGAAATT
    TTAGCAGTTG ATCTGCCAAA AGAAACAATA CAGAACTTGT TAGCTTTAGA GTGCGCAGAT
    GAACAAGTCT TCAATGCATC AGTGAAAATG CTAATGTCTC GGCTGCCAAA ACAGAGATAC
    CTGAAATCAG TGTTTGATGA AATATACACC ATTAAAATCG AAAAGCGGGT TCCTGTGCTG
    GTACTATACA GTTATAAAGA TGATTATTAC AAGATCCTTT TTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012823581.1
    CDS648..5672
    Translation

    Target ORF information:

    RefSeq Version XM_012968127.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis eukaryotic translation initiation factor 2 alpha kinase 4 (eif2ak4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012968127.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGCAGGCG GCCGGGGAAA TGCACTTCTT CCTATTGAGC TCAGCCCGCA AGGGGGTCTG 
    CGCCCTACTC CGCCTGCCTG TGACCCCCCT GAAACTTATT CCCACCGGCA GGAGAATGAG
    CTGGAGGTCC TGGAATCCAT CTATAGTGGG GATTTCGCGG ATCTGCGCAA GAACTCTGCC
    TGGAAGGTAC AGCAGCCTCC AGAAGTCATC ATTGCCTTAC GTCCTCAGGG AAATGAGGTT
    CATGTCAAGG TGGATTTATG GGTAAAATGC CCTCAGAACT ATCCTGATGT TCCTCCTGAA
    ATAAAGCTGA AGAACATCCA GGGGTTGTCC AATGAAAATG TGAACTCTCT GACATCAAGC
    TTGGAAGATT TAGCAAAACA GCTTTGTGGA GAGGTAATGA TATACCAGCT TGCGGATCAT
    GTGCAGTGTT TTCTTACAGA GTATAACCAT CCACCTCCTA AATCCTTTCA TGAGGAGATG
    TTAAAAAACC TTCAGAAGCA GCAGGAGAAG CTTGCTCTTG AAGAAGAGAG ACGAATGCAA
    GAGATAAAGA TGAAAGAAGA GCAGACGAGA CGGGAAATCC TTAATGAAAT CCAGAAGCGA
    GAAGAAGAGA AAAAGGAAGA AAAAAGAAGG AAGGAAGTAG CAAAACAGGA ACGGATTGAA
    AACGCTAATC TAATGTGCCA AGAGAATGTA AATAAGATTA ATAATACAGG CAAGACAACA
    ACAGCTGCCT CGGATGGGTC ACTTCCTGAT CATGGAGGAA CCAACAGAAA TCGGCCACAT
    TCAGCAGGAC GCTCAAAGCG GGACCGACAG TATTCGGTGT GCCTCACTGA TGACTCAACC
    CCATTTGAAG TGTTAACCTT TGATATTGGA TGCAGTGGGC AAATTGTTGT TCACAGAGGA
    AAGTGCCTGG GGAAAGATGA GCTGCTGGGT AAATCTGTTT ACAATGGCTT GGAAACTGCT
    TCAGGACATT TTGTTGTAAT CTATGAATGG GTTCTCCAGT GGCAGAAAAA GATGGGCAAG
    TTTCTTACTA ATCAAGAAAA AGAAAAGATT GAAAAGTGTA AAAAACAGAT TCAGGGAACA
    GAAACCGAGT TCAGTTCTCT TATCAAACTG AGCCATCCAA ACATTGTGCA CTACCTCGCT
    ATGCGAATAA AAGAGAAAGA AGATTCCATT TCTGTCGGGA TCATAGCTGA ACATGTTAAT
    GGTACAAAGC TTTCCAGTTA CCTTGAGCAG GGTGTACCTG TTCCACTGGA TCAACTACGG
    CACTATACTG CCCAGCTGCT ATCGGCACTG GACTATTTGC ATTCTAATTC TGTGGTACAT
    AAAGTCATGT GCGCCTCCAG TGTTTTGGTG ACAGCTGACG GGAAAGTAAA ACTGACAGAC
    TATAGCCTTT CCAAACGCCT TGCTGACATC TGCAAAGAGG ACGTGTTTGA GCAAACAAAA
    GTTCGATTCA GTGAGGATGC TCTGCCTAGC AAGAGTGGCA AGAAAGGAGA TATATGGAAG
    CTCGGTCTCT TGCTACTTTC GCTGTCACAA GGCAACATAG CCAAAGAGTA TCCTGTAAAA
    GTGCCCAGTG ACCTGCCTGC AGATTTCCAA GATTTCCTGC AAAAGTGTGT TTGCTTGGAA
    GACAAGGAAC GCTGGACTAC ACAGCAGTTA CTAAGTCACA GTTTCCTTAA CCCTCCACCT
    GAGAAGAGTC CTGTAACAGA GGAATGCTCA GATGATTATC CGGGTGACTG TACTGAGACT
    GTCATTCCCA GATCTTCAGT ATCTAATTCC ACATTCCATA TTGAGTCACA GAGACAGTTT
    TCTCGCTACT TCAATGAGTT TGAAGAATTA CAGATGCTTG GGAAAGGGGC ATTTGGTGCT
    GTCATTAAGG TTCAGAACAA ACTGGATGGC TGCTATTACG CTGTAAAACG GGTACAAATC
    AATCCTGCAA GTAAACAGTT CCGCAGAATT AAGGGGGAGG TAACATTGTT ATCTCGGCTC
    AACCATGAAA ACATTGTAAG GTACTATAAT GCATGGATTG AGAAACACGA GCATCCTACT
    ATTTCTCCTA AATGTGAAGC ACATGAGGAA AAGAAAGTGA CTGTGAAGCC AGAACAAATA
    GTAAAGAATG ATTTGGGATT GTTGGATGAT ATTGAGGAAA ACGCACCAGC ACCCATATTA
    ACCAGCTCTG TAGAATGGAG TACATCTTGC GAGCGATCAT CCAGTGCGCA CTGTACTAAT
    GGGGATCAAG ATTCAAGTGA TGATGATGAT GATGACGATG AAGAGGGAGT GTTTTCACCT
    TCGTTCCTGC CTGGGACAGA TTCTGACAGT GAGATCATAT TTGAGAATGA AGATGACATT
    AGTCCAGATC AGGATGAAGA ATATGACAAG AACAATGTAG AAGACCGCAA AGCTGGAGCC
    AATGAGATTG TGACTCAGGC AGTGCATTAT TTATATATTC AGATGGAATA TTGTGAGAAA
    AGCACCTTAC GGGACACGAT AGACCAAGGA CTGTATATGG ATGTTGGCCG CCTCTGGAGG
    CTGTTTCGTG AAATTTTGGA TGGATTGACC TATATACATG AGCAGGGAAT GATCCACAGA
    GACCTAAAGC CAGTCAATAT CTTTTTAGAT TCAGAAGATC ATGTCAAAAT AGGAGATTTT
    GGACTAGCTA CAGATCACCC AGCATCTTAT ACTGAATCAG CACAAGGGGA AGAGGATCAG
    GCGCATTCTG ACTTGGTTAA AGTCGACCCT GAAGATAGGG CCTCAACACC TGGTCGCAAT
    ACTCAAGGAA AACTCACTGG CATGGTTGGC ACTGCCTTGT ATGTAAGCCC TGAAGTCCAA
    GGAAACACCA AAGCTTCATA TAACCAGAAA GTGGACCTTT ACAGCCTGGG TATAATATTG
    TTTGAGATGT CCTACCGGCC CATGGACACA AGTTCTGAAC GTATCTCTGT CTTAAGCAGG
    CTTAGACAGG CCTTGATTGT GTTCCCAAGT GATTTTGAAA ACCCAGAAAA TGAAAAACAG
    AAAAAAGTGA TTACTTGGCT CCTGAACCAT GACCCAGCAT CCCGCCCCAC CGCTATGGAG
    TTGCTTAAGT GCGATCTCTT ACCCCCACCA CAACTAGAGG AGTCTGAACT CCATGAAGTC
    CTGCACCACA CCTTGGCCAA TATCGATGGG AAAGCGTACC GCACAATGAT GAGTCAAATC
    TTTGCCCAGC GCATTTCACC TGCTATGGAT TATACATATG ACAGTGATAT ATTGAAGGGT
    ATTTTCTCAG TCCGTTCTGC CAGACTACAT CATCATGTTT GTGAGGCAGT CTGCAGGATA
    TTTAAAAGAC ACGGGGCTGT GAAGTTACAT GCCCCACTGC TGATGCCACG AGGTAAAGCT
    GGGTTTGATA ACAGTGAATC TGCATGTTTT ATGGATCACA GTGGCATGCT GGTCACCCTT
    CCTTATAACC TCAGGATATC ATTTGCGCGG TTTGTTGCAA GGAATAATAT AACTAGTTTG
    AAAAGGTATA GCATTGAGAG AGTGTTTAGA CCACGCAAAC TGAACCGCTG CCATCCAAAG
    GAACTCACTG AGTGTGCATT TGATATAATC ACTTCTGCTG CAAACTGTTA CCTGCCCATT
    GCAGAAACAA TATACACCAT ATATGAAATT ATCCAGGAAT TTCCAGTACT GCAGGAAAGG
    AATTACAGTA TTTATGTGAA CCACACCTCG TTGTTGAAAG CCATTCTCCT TCACTGTGGC
    GTGCCCGAGG ATAAACTCAC TCAGGTGTAT AACATTCTGT ATGATGCAAT GATTGAGAAG
    CTGACAAAAA GAGAAGTGGA AGCCAAATTT TGTAATCTCT CCCTTTCAGA GAACAGTTTC
    CAATTGAGTC GCCTTTACCG GTTTATTGAG CAGAAAGGAG ACCTCCCAGA TTTGGAGGCA
    ATGATGGTCC CACTCACAAA GCAAAAAACA GGGGTCGGTG TGCTGGCAAA GCAAGGTTTG
    AAAGACTTGG AAGGAGTCAT TGGATTAGTG AAGAGGCTGG GCATTAAATT ACAGATTGTG
    GTTAACTTTG GACTTGTCTA CAAGATGCAG CAGCACAGTG GCATAGTCTT CCAGTTTGTG
    GCTTTTATCA AGCGGAGACA GAGGACTGTG CCTGAAATAC TTGCAGCTGG AGGCAGATAT
    GACCATTTGA TTTCCCAGTT TAGGGGACCT CAGACTGGAG CTATGGGATT ACCCTGTGCT
    GTAGGCGTTA GTATAGCAAT AGACAAGATA TCTGCTGCGC TTTCCACTGC AGAGGAACAT
    ATCAGCAGCT GTGATTTATT AGTCGTAAGC GCTGGGCAGA TGTCTCTGAA TAGAGCCATT
    GGGATAACAC AGAAGCTGTG GGCTGCTGGC ATTACTGCAG AGATCATGTA TGAGTGGTCT
    GAGTCTCAGG ATGAACTGCA GGATTATTGC AAGTGTTCGG GGATTTCGTA TGCTGCAATT
    ATTTCTGACA AAGAAGGAAA TCACATTAAG CTGAAAACAT TTGAGAAGGA AAGACAGAGT
    GAGAAGAGAA TATTGGAATC TGATCTAGTG GATCTCCTGG TGCAGAAACT TCGGATCAGA
    GTCCTTGAAG AAAGAAATAA CAGAGAAACG TCGGACAGTC TCTCAGTGCA AAATGTGAAG
    GGCTCGTTTA ATTCCCAGAG TCAGTTTGAG CCACACAGCG CAATAATTGT GCCAAGTGTC
    AGTGTTATAG CACCAGAAAA GCTATCTGCC AGTGCCAGGC GGAGATTTGA AACCCAGGTG
    CTAACAAAGC TTCAGACACT CATTACAAAC TTCCAGCAGA GAAATGGTGA AATTGAAATT
    TTAGCAGTTG ATCTGCCAAA AGAAACAATA CAGAACTTGT TAGCTTTAGA GTGCGCAGAT
    GAACAAGTCT TCAATGCATC AGTGAAAATG CTAATGTCTC GGCTGCCAAA ACAGAGATAC
    CTGAAATCAG TGTTTGATGA AATATACACC ATTAAAATCG AAAAGCGGGT TCCTGTGCTG
    GTACTATACA GTTATAAAGA TGATTATTAC AAGATCCTTT TTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32795
    Clone ID Related Accession (Same CDS sequence) XM_012968128.3 , XM_012968128.2
    Accession Version XM_012968128.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4992bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product eIF-2-alpha kinase GCN2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968128.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGCAGGCG GCCGGGGAAA TGCACTTCTT CCTATTGAGC TCAGCCCGCA AGGGGGTCTG 
    CGCCCTACTC CGCCTGCCTG TGACCCCCCT GAAACTTATT CCCACCGGCA GGAGAATGAG
    CTGGAGGTCC TGGAATCCAT CTATAGTGGG GATTTCGCGG ATCTGCGCAA GAACTCTGCC
    TGGAAGGTAC AGCAGCCTCC AGAAGTCATC ATTGCCTTAC GTCCTCAGGG AAATGAGGTT
    CATGTCAAGG TGGATTTATG GGTAAAATGC CCTCAGAACT ATCCTGATGT TCCTCCTGAA
    ATAAAGCTGA AGAACATCCA GGGGTTGTCC AATGAAAATG TGAACTCTCT GACATCAAGC
    TTGGAAGATT TAGCAAAACA GCTTTGTGGA GAGGTAATGA TATACCAGCT TGCGGATCAT
    GTGCAGTGTT TTCTTACAGA GTATAACCAT CCACCTCCTA AATCCTTTCA TGAGGAGATG
    TTAAAAAACC TTCAGAAGCA GCAGGAGAAG CTTGCTCTTG AAGAAGAGAG ACGAATGCAA
    GAGATAAAGA TGAAAGAAGA GCAGACGAGA CGGGAAATCC TTAATGAAAT CCAGAAGCGA
    GAAGAAGAGA AAAAGGAAGA AAAAAGAAGG AAGGAAGTAG CAAAACAGGA ACGGATTGAA
    AACGCTAATC TAATGTGCCA AGAGAATGTA AATAAGATTA ATAATACAGG CAAGACAACA
    ACAGCTGCCT CGGATGGGTC ACTTCCTGAT CATGGAGGAA CCAACAGAAA TCGGCCACAT
    TCAGCAGGAC GCTCAAAGCG GGACCGACAG TATTCGGTGT GCCTCACTGA TGACTCAACC
    CCATTTGAAG TGTTAACCTT TGATATTGGA TGCAGTGGGC AAATTGTTGT TCACAGAGGA
    AAGTGCCTGG GGAAAGATGA GCTGCTGGGT AAATCTGTTT ACAATGGCTT GGAAACTGCT
    TCAGGACATT TTGTTGTAAT CTATGAATGG GTTCTCCAGT GGCAGAAAAA GATGGGCAAG
    TTTCTTACTA ATCAAGAAAA AGAAAAGATT GAAAAGTGTA AAAAACAGAT TCAGGGAACA
    GAAACCGAGT TCAGTTCTCT TATCAAACTG AGCCATCCAA ACATTGTGCA CTACCTCGCT
    ATGCGAATAA AAGAGAAAGA AGATTCCATT TCTGTCGGGA TCATAGCTGA ACATGTTAAT
    GGTACAAAGC TTTCCAGTTA CCTTGAGCAG GGTGTACCTG TTCCACTGGA TCAACTACGG
    CACTATACTG CCCAGCTGCT ATCGGCACTG GACTATTTGC ATTCTAATTC TGTGGTACAT
    AAAGTCATGT GCGCCTCCAG TGTTTTGGTG ACAGCTGACG GGAAAGTAAA ACTGACAGAC
    TATAGCCTTT CCAAACGCCT TGCTGACATC TGCAAAGAGG ACGTGTTTGA GCAAACAAAA
    GTTCGATTCA GTGAGGATGC TCTGCCTAGC AAGAGTGGCA AGAAAGGAGA TATATGGAAG
    CTCGGTCTCT TGCTACTTTC GCTGTCACAA GGCAACATAG CCAAAGAGTA TCCTGTAAAA
    GTGCCCAGTG ACCTGCCTGC AGATTTCCAA GATTTCCTGC AAAAGTGTGT TTGCTTGGAA
    GACAAGGAAC GCTGGACTAC ACAGCAGTTA CTAAGTCACA GTTTCCTTAA CCCTCCACCT
    GAGAAGAGTC CTGTAACAGA GGAATGCTCA GATGATTATC CGGGTGACTG TACTGAGACT
    GTCATTCCCA GATCTTCAGT ATCTAATTCC ACATTCCATA TTGAGTCACA GAGACAGTTT
    TCTCGCTACT TCAATGAGTT TGAAGAATTA CAGATGCTTG GGAAAGGGGC ATTTGGTGCT
    GTCATTAAGG TTCAGAACAA ACTGGATGGC TGCTATTACG CTGTAAAACG GGTACAAATC
    AATCCTGCAA GTAAACAGTT CCGCAGAATT AAGGGGGAGG TAACATTGTT ATCTCGGCTC
    AACCATGAAA ACATTGTAAG GTACTATAAT GCATGGATTG AGAAACACGA GCATCCTACT
    ATTTCTCCTA AATGTGAAGC ACATGAGGAA AAGAAAGTGA CTGTGAAGCC AGAACAAATA
    GTAAAGAATG ATTTGGGATT GTTGGATGAT ATTGAGGAAA ACGCACCAGC ACCCATATTA
    ACCAGCTCTG TAGAATGGAG TACATCTTGC GAGCGATCAT CCAGTGCGCA CTGTACTAAT
    GGGGATCAAG ATTCAAGTGA TGATGATGAT GATGACGATG AAGAGGGAGT GTTTTCACCT
    TCGTTCCTGC CTGGGACAGA TTCTGACAGT GAGATCATAT TTGAGAATGA AGATGACATT
    AGTCCAGATC AGGATGAAGA ATATGACAAG AACAATGTAG AAGACCGCAA AGCTGGAGCC
    AATGAGATTG TGACTCAGGC AGTGCATTAT TTATATATTC AGATGGAATA TTGTGAGAAA
    AGCACCTTAC GGGACACGAT AGACCAAGGA CTGTATATGG ATGTTGGCCG CCTCTGGAGG
    CTGTTTCGTG AAATTTTGGA TGGATTGACC TATATACATG AGCAGGGAAT GATCCACAGA
    GACCTAAAGC CAGTCAATAT CTTTTTAGAT TCAGAAGATC ATGTCAAAAT AGGAGATTTT
    GGACTAGCTA CAGATCACCC AGCATCTTAT ACTGAATCAG CACAAGGGGA AGAGGATCAG
    GCGCATTCTG ACTTGGTTAA AGTCGACCCT GAAGGAAAAC TCACTGGCAT GGTTGGCACT
    GCCTTGTATG TAAGCCCTGA AGTCCAAGGA AACACCAAAG CTTCATATAA CCAGAAAGTG
    GACCTTTACA GCCTGGGTAT AATATTGTTT GAGATGTCCT ACCGGCCCAT GGACACAAGT
    TCTGAACGTA TCTCTGTCTT AAGCAGGCTT AGACAGGCCT TGATTGTGTT CCCAAGTGAT
    TTTGAAAACC CAGAAAATGA AAAACAGAAA AAAGTGATTA CTTGGCTCCT GAACCATGAC
    CCAGCATCCC GCCCCACCGC TATGGAGTTG CTTAAGTGCG ATCTCTTACC CCCACCACAA
    CTAGAGGAGT CTGAACTCCA TGAAGTCCTG CACCACACCT TGGCCAATAT CGATGGGAAA
    GCGTACCGCA CAATGATGAG TCAAATCTTT GCCCAGCGCA TTTCACCTGC TATGGATTAT
    ACATATGACA GTGATATATT GAAGGGTATT TTCTCAGTCC GTTCTGCCAG ACTACATCAT
    CATGTTTGTG AGGCAGTCTG CAGGATATTT AAAAGACACG GGGCTGTGAA GTTACATGCC
    CCACTGCTGA TGCCACGAGG TAAAGCTGGG TTTGATAACA GTGAATCTGC ATGTTTTATG
    GATCACAGTG GCATGCTGGT CACCCTTCCT TATAACCTCA GGATATCATT TGCGCGGTTT
    GTTGCAAGGA ATAATATAAC TAGTTTGAAA AGGTATAGCA TTGAGAGAGT GTTTAGACCA
    CGCAAACTGA ACCGCTGCCA TCCAAAGGAA CTCACTGAGT GTGCATTTGA TATAATCACT
    TCTGCTGCAA ACTGTTACCT GCCCATTGCA GAAACAATAT ACACCATATA TGAAATTATC
    CAGGAATTTC CAGTACTGCA GGAAAGGAAT TACAGTATTT ATGTGAACCA CACCTCGTTG
    TTGAAAGCCA TTCTCCTTCA CTGTGGCGTG CCCGAGGATA AACTCACTCA GGTGTATAAC
    ATTCTGTATG ATGCAATGAT TGAGAAGCTG ACAAAAAGAG AAGTGGAAGC CAAATTTTGT
    AATCTCTCCC TTTCAGAGAA CAGTTTCCAA TTGAGTCGCC TTTACCGGTT TATTGAGCAG
    AAAGGAGACC TCCCAGATTT GGAGGCAATG ATGGTCCCAC TCACAAAGCA AAAAACAGGG
    GTCGGTGTGC TGGCAAAGCA AGGTTTGAAA GACTTGGAAG GAGTCATTGG ATTAGTGAAG
    AGGCTGGGCA TTAAATTACA GATTGTGGTT AACTTTGGAC TTGTCTACAA GATGCAGCAG
    CACAGTGGCA TAGTCTTCCA GTTTGTGGCT TTTATCAAGC GGAGACAGAG GACTGTGCCT
    GAAATACTTG CAGCTGGAGG CAGATATGAC CATTTGATTT CCCAGTTTAG GGGACCTCAG
    ACTGGAGCTA TGGGATTACC CTGTGCTGTA GGCGTTAGTA TAGCAATAGA CAAGATATCT
    GCTGCGCTTT CCACTGCAGA GGAACATATC AGCAGCTGTG ATTTATTAGT CGTAAGCGCT
    GGGCAGATGT CTCTGAATAG AGCCATTGGG ATAACACAGA AGCTGTGGGC TGCTGGCATT
    ACTGCAGAGA TCATGTATGA GTGGTCTGAG TCTCAGGATG AACTGCAGGA TTATTGCAAG
    TGTTCGGGGA TTTCGTATGC TGCAATTATT TCTGACAAAG AAGGAAATCA CATTAAGCTG
    AAAACATTTG AGAAGGAAAG ACAGAGTGAG AAGAGAATAT TGGAATCTGA TCTAGTGGAT
    CTCCTGGTGC AGAAACTTCG GATCAGAGTC CTTGAAGAAA GAAATAACAG AGAAACGTCG
    GACAGTCTCT CAGTGCAAAA TGTGAAGGGC TCGTTTAATT CCCAGAGTCA GTTTGAGCCA
    CACAGCGCAA TAATTGTGCC AAGTGTCAGT GTTATAGCAC CAGAAAAGCT ATCTGCCAGT
    GCCAGGCGGA GATTTGAAAC CCAGGTGCTA ACAAAGCTTC AGACACTCAT TACAAACTTC
    CAGCAGAGAA ATGGTGAAAT TGAAATTTTA GCAGTTGATC TGCCAAAAGA AACAATACAG
    AACTTGTTAG CTTTAGAGTG CGCAGATGAA CAAGTCTTCA ATGCATCAGT GAAAATGCTA
    ATGTCTCGGC TGCCAAAACA GAGATACCTG AAATCAGTGT TTGATGAAAT ATACACCATT
    AAAATCGAAA AGCGGGTTCC TGTGCTGGTA CTATACAGTT ATAAAGATGA TTATTACAAG
    ATCCTTTTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012823582.1
    CDS647..5638
    Translation

    Target ORF information:

    RefSeq Version XM_012968128.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis eukaryotic translation initiation factor 2 alpha kinase 4 (eif2ak4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012968128.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGCAGGCG GCCGGGGAAA TGCACTTCTT CCTATTGAGC TCAGCCCGCA AGGGGGTCTG 
    CGCCCTACTC CGCCTGCCTG TGACCCCCCT GAAACTTATT CCCACCGGCA GGAGAATGAG
    CTGGAGGTCC TGGAATCCAT CTATAGTGGG GATTTCGCGG ATCTGCGCAA GAACTCTGCC
    TGGAAGGTAC AGCAGCCTCC AGAAGTCATC ATTGCCTTAC GTCCTCAGGG AAATGAGGTT
    CATGTCAAGG TGGATTTATG GGTAAAATGC CCTCAGAACT ATCCTGATGT TCCTCCTGAA
    ATAAAGCTGA AGAACATCCA GGGGTTGTCC AATGAAAATG TGAACTCTCT GACATCAAGC
    TTGGAAGATT TAGCAAAACA GCTTTGTGGA GAGGTAATGA TATACCAGCT TGCGGATCAT
    GTGCAGTGTT TTCTTACAGA GTATAACCAT CCACCTCCTA AATCCTTTCA TGAGGAGATG
    TTAAAAAACC TTCAGAAGCA GCAGGAGAAG CTTGCTCTTG AAGAAGAGAG ACGAATGCAA
    GAGATAAAGA TGAAAGAAGA GCAGACGAGA CGGGAAATCC TTAATGAAAT CCAGAAGCGA
    GAAGAAGAGA AAAAGGAAGA AAAAAGAAGG AAGGAAGTAG CAAAACAGGA ACGGATTGAA
    AACGCTAATC TAATGTGCCA AGAGAATGTA AATAAGATTA ATAATACAGG CAAGACAACA
    ACAGCTGCCT CGGATGGGTC ACTTCCTGAT CATGGAGGAA CCAACAGAAA TCGGCCACAT
    TCAGCAGGAC GCTCAAAGCG GGACCGACAG TATTCGGTGT GCCTCACTGA TGACTCAACC
    CCATTTGAAG TGTTAACCTT TGATATTGGA TGCAGTGGGC AAATTGTTGT TCACAGAGGA
    AAGTGCCTGG GGAAAGATGA GCTGCTGGGT AAATCTGTTT ACAATGGCTT GGAAACTGCT
    TCAGGACATT TTGTTGTAAT CTATGAATGG GTTCTCCAGT GGCAGAAAAA GATGGGCAAG
    TTTCTTACTA ATCAAGAAAA AGAAAAGATT GAAAAGTGTA AAAAACAGAT TCAGGGAACA
    GAAACCGAGT TCAGTTCTCT TATCAAACTG AGCCATCCAA ACATTGTGCA CTACCTCGCT
    ATGCGAATAA AAGAGAAAGA AGATTCCATT TCTGTCGGGA TCATAGCTGA ACATGTTAAT
    GGTACAAAGC TTTCCAGTTA CCTTGAGCAG GGTGTACCTG TTCCACTGGA TCAACTACGG
    CACTATACTG CCCAGCTGCT ATCGGCACTG GACTATTTGC ATTCTAATTC TGTGGTACAT
    AAAGTCATGT GCGCCTCCAG TGTTTTGGTG ACAGCTGACG GGAAAGTAAA ACTGACAGAC
    TATAGCCTTT CCAAACGCCT TGCTGACATC TGCAAAGAGG ACGTGTTTGA GCAAACAAAA
    GTTCGATTCA GTGAGGATGC TCTGCCTAGC AAGAGTGGCA AGAAAGGAGA TATATGGAAG
    CTCGGTCTCT TGCTACTTTC GCTGTCACAA GGCAACATAG CCAAAGAGTA TCCTGTAAAA
    GTGCCCAGTG ACCTGCCTGC AGATTTCCAA GATTTCCTGC AAAAGTGTGT TTGCTTGGAA
    GACAAGGAAC GCTGGACTAC ACAGCAGTTA CTAAGTCACA GTTTCCTTAA CCCTCCACCT
    GAGAAGAGTC CTGTAACAGA GGAATGCTCA GATGATTATC CGGGTGACTG TACTGAGACT
    GTCATTCCCA GATCTTCAGT ATCTAATTCC ACATTCCATA TTGAGTCACA GAGACAGTTT
    TCTCGCTACT TCAATGAGTT TGAAGAATTA CAGATGCTTG GGAAAGGGGC ATTTGGTGCT
    GTCATTAAGG TTCAGAACAA ACTGGATGGC TGCTATTACG CTGTAAAACG GGTACAAATC
    AATCCTGCAA GTAAACAGTT CCGCAGAATT AAGGGGGAGG TAACATTGTT ATCTCGGCTC
    AACCATGAAA ACATTGTAAG GTACTATAAT GCATGGATTG AGAAACACGA GCATCCTACT
    ATTTCTCCTA AATGTGAAGC ACATGAGGAA AAGAAAGTGA CTGTGAAGCC AGAACAAATA
    GTAAAGAATG ATTTGGGATT GTTGGATGAT ATTGAGGAAA ACGCACCAGC ACCCATATTA
    ACCAGCTCTG TAGAATGGAG TACATCTTGC GAGCGATCAT CCAGTGCGCA CTGTACTAAT
    GGGGATCAAG ATTCAAGTGA TGATGATGAT GATGACGATG AAGAGGGAGT GTTTTCACCT
    TCGTTCCTGC CTGGGACAGA TTCTGACAGT GAGATCATAT TTGAGAATGA AGATGACATT
    AGTCCAGATC AGGATGAAGA ATATGACAAG AACAATGTAG AAGACCGCAA AGCTGGAGCC
    AATGAGATTG TGACTCAGGC AGTGCATTAT TTATATATTC AGATGGAATA TTGTGAGAAA
    AGCACCTTAC GGGACACGAT AGACCAAGGA CTGTATATGG ATGTTGGCCG CCTCTGGAGG
    CTGTTTCGTG AAATTTTGGA TGGATTGACC TATATACATG AGCAGGGAAT GATCCACAGA
    GACCTAAAGC CAGTCAATAT CTTTTTAGAT TCAGAAGATC ATGTCAAAAT AGGAGATTTT
    GGACTAGCTA CAGATCACCC AGCATCTTAT ACTGAATCAG CACAAGGGGA AGAGGATCAG
    GCGCATTCTG ACTTGGTTAA AGTCGACCCT GAAGGAAAAC TCACTGGCAT GGTTGGCACT
    GCCTTGTATG TAAGCCCTGA AGTCCAAGGA AACACCAAAG CTTCATATAA CCAGAAAGTG
    GACCTTTACA GCCTGGGTAT AATATTGTTT GAGATGTCCT ACCGGCCCAT GGACACAAGT
    TCTGAACGTA TCTCTGTCTT AAGCAGGCTT AGACAGGCCT TGATTGTGTT CCCAAGTGAT
    TTTGAAAACC CAGAAAATGA AAAACAGAAA AAAGTGATTA CTTGGCTCCT GAACCATGAC
    CCAGCATCCC GCCCCACCGC TATGGAGTTG CTTAAGTGCG ATCTCTTACC CCCACCACAA
    CTAGAGGAGT CTGAACTCCA TGAAGTCCTG CACCACACCT TGGCCAATAT CGATGGGAAA
    GCGTACCGCA CAATGATGAG TCAAATCTTT GCCCAGCGCA TTTCACCTGC TATGGATTAT
    ACATATGACA GTGATATATT GAAGGGTATT TTCTCAGTCC GTTCTGCCAG ACTACATCAT
    CATGTTTGTG AGGCAGTCTG CAGGATATTT AAAAGACACG GGGCTGTGAA GTTACATGCC
    CCACTGCTGA TGCCACGAGG TAAAGCTGGG TTTGATAACA GTGAATCTGC ATGTTTTATG
    GATCACAGTG GCATGCTGGT CACCCTTCCT TATAACCTCA GGATATCATT TGCGCGGTTT
    GTTGCAAGGA ATAATATAAC TAGTTTGAAA AGGTATAGCA TTGAGAGAGT GTTTAGACCA
    CGCAAACTGA ACCGCTGCCA TCCAAAGGAA CTCACTGAGT GTGCATTTGA TATAATCACT
    TCTGCTGCAA ACTGTTACCT GCCCATTGCA GAAACAATAT ACACCATATA TGAAATTATC
    CAGGAATTTC CAGTACTGCA GGAAAGGAAT TACAGTATTT ATGTGAACCA CACCTCGTTG
    TTGAAAGCCA TTCTCCTTCA CTGTGGCGTG CCCGAGGATA AACTCACTCA GGTGTATAAC
    ATTCTGTATG ATGCAATGAT TGAGAAGCTG ACAAAAAGAG AAGTGGAAGC CAAATTTTGT
    AATCTCTCCC TTTCAGAGAA CAGTTTCCAA TTGAGTCGCC TTTACCGGTT TATTGAGCAG
    AAAGGAGACC TCCCAGATTT GGAGGCAATG ATGGTCCCAC TCACAAAGCA AAAAACAGGG
    GTCGGTGTGC TGGCAAAGCA AGGTTTGAAA GACTTGGAAG GAGTCATTGG ATTAGTGAAG
    AGGCTGGGCA TTAAATTACA GATTGTGGTT AACTTTGGAC TTGTCTACAA GATGCAGCAG
    CACAGTGGCA TAGTCTTCCA GTTTGTGGCT TTTATCAAGC GGAGACAGAG GACTGTGCCT
    GAAATACTTG CAGCTGGAGG CAGATATGAC CATTTGATTT CCCAGTTTAG GGGACCTCAG
    ACTGGAGCTA TGGGATTACC CTGTGCTGTA GGCGTTAGTA TAGCAATAGA CAAGATATCT
    GCTGCGCTTT CCACTGCAGA GGAACATATC AGCAGCTGTG ATTTATTAGT CGTAAGCGCT
    GGGCAGATGT CTCTGAATAG AGCCATTGGG ATAACACAGA AGCTGTGGGC TGCTGGCATT
    ACTGCAGAGA TCATGTATGA GTGGTCTGAG TCTCAGGATG AACTGCAGGA TTATTGCAAG
    TGTTCGGGGA TTTCGTATGC TGCAATTATT TCTGACAAAG AAGGAAATCA CATTAAGCTG
    AAAACATTTG AGAAGGAAAG ACAGAGTGAG AAGAGAATAT TGGAATCTGA TCTAGTGGAT
    CTCCTGGTGC AGAAACTTCG GATCAGAGTC CTTGAAGAAA GAAATAACAG AGAAACGTCG
    GACAGTCTCT CAGTGCAAAA TGTGAAGGGC TCGTTTAATT CCCAGAGTCA GTTTGAGCCA
    CACAGCGCAA TAATTGTGCC AAGTGTCAGT GTTATAGCAC CAGAAAAGCT ATCTGCCAGT
    GCCAGGCGGA GATTTGAAAC CCAGGTGCTA ACAAAGCTTC AGACACTCAT TACAAACTTC
    CAGCAGAGAA ATGGTGAAAT TGAAATTTTA GCAGTTGATC TGCCAAAAGA AACAATACAG
    AACTTGTTAG CTTTAGAGTG CGCAGATGAA CAAGTCTTCA ATGCATCAGT GAAAATGCTA
    ATGTCTCGGC TGCCAAAACA GAGATACCTG AAATCAGTGT TTGATGAAAT ATACACCATT
    AAAATCGAAA AGCGGGTTCC TGTGCTGGTA CTATACAGTT ATAAAGATGA TTATTACAAG
    ATCCTTTTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.