eif2ak4 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	eif2ak4
Entrez Gene ID	100216307
Full Name	eukaryotic translation initiation factor 2 alpha kinase 4
General protein information	Preferred Names eukaryotic translation initiation factor 2 alpha kinase 4 Names eIF-2-alpha kinase GCN2 GCN2 protein
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012968127.2	XP_012823581.1	eIF-2-alpha kinase GCN2 isoform X1
XM_018096178.1	XP_017951667.1	eIF-2-alpha kinase GCN2 isoform X3
NM_001256926.1	NP_001243855.1	eIF-2-alpha kinase GCN2
XM_012968127.3	XP_012823581.1	eIF-2-alpha kinase GCN2 isoform X1
XM_012968128.3	XP_012823582.1	eIF-2-alpha kinase GCN2 isoform X2
XM_012968128.2	XP_012823582.1	eIF-2-alpha kinase GCN2 isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	EIF2AK4	XP_001140245.1
	EIF2AK4	XP_002804763.1
Gallus gallus (chicken)	EIF2AK4	XP_004941777.1
Eremothecium gossypii	AGOS_AAL083W	NP_982459.1
Rattus norvegicus (Norway rat)	Eif2ak4	NP_001099214.2
Danio rerio (zebrafish)	eif2ak4	XP_002667149.3
Drosophila melanogaster (fruit fly)	Gcn2	NP_001263141.1
Saccharomyces cerevisiae (baker's yeast)	GCN2	NP_010569.3
Kluyveromyces lactis	KLLA0C10802g	XP_452681.1
Neurospora crassa	NCU01187	XP_961553.1
Xenopus tropicalis (tropical clawed frog)	eif2ak4	NP_001243855.1
Homo sapiens (human)	EIF2AK4	NP_001013725.2
Canis lupus familiaris (dog)	EIF2AK4	XP_535431.3
Bos taurus (cattle)	EIF2AK4	XP_002690831.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012368	XP_320188.4
Caenorhabditis elegans (roundworm)	gcn-2	NP_001254370.1
Schizosaccharomyces pombe (fission yeast)	gcn2	NP_595991.2
Mus musculus (house mouse)	Eif2ak4	NP_038747.2
Magnaporthe oryzae (rice blast fungus)	MGG_08643	XP_003711074.1

Gene Ontology

The following eif2ak4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the eif2ak4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa32794	XM_012968127.3 Latest version!	Xenopus tropicalis eukaryotic translation initiation factor 2 alpha kinase 4 (eif2ak4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa32795	XM_012968128.3 Latest version!	Xenopus tropicalis eukaryotic translation initiation factor 2 alpha kinase 4 (eif2ak4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa45985	XM_018096178.1 Latest version!	Xenopus tropicalis eukaryotic translation initiation factor 2 alpha kinase 4 (eif2ak4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OXa20310	NM_001256926.1 Latest version!	Xenopus tropicalis eukaryotic translation initiation factor 2 alpha kinase 4 (eif2ak4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
OXa32794	XM_012968127.2	Xenopus tropicalis eukaryotic translation initiation factor 2 alpha kinase 4 (eif2ak4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa32795	XM_012968128.2	Xenopus tropicalis eukaryotic translation initiation factor 2 alpha kinase 4 (eif2ak4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

eif2ak4 ( XM_012968127.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823581.1 Xenopus tropicalis eukaryotic translation initiation factor 2 alpha kinase 4 (eif2ak4), transcript variant X1, mRNA.

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CloneID	OXa32794
Clone ID Related Accession (Same CDS sequence)	XM_012968127.2 , XM_012968127.3
Accession Version	XM_012968127.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5025bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	eIF-2-alpha kinase GCN2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012968127.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGCG GCCGGGGAAA TGCACTTCTT CCTATTGAGC TCAGCCCGCA AGGGGGTCTG 
CGCCCTACTC CGCCTGCCTG TGACCCCCCT GAAACTTATT CCCACCGGCA GGAGAATGAG 
CTGGAGGTCC TGGAATCCAT CTATAGTGGG GATTTCGCGG ATCTGCGCAA GAACTCTGCC 
TGGAAGGTAC AGCAGCCTCC AGAAGTCATC ATTGCCTTAC GTCCTCAGGG AAATGAGGTT 
CATGTCAAGG TGGATTTATG GGTAAAATGC CCTCAGAACT ATCCTGATGT TCCTCCTGAA 
ATAAAGCTGA AGAACATCCA GGGGTTGTCC AATGAAAATG TGAACTCTCT GACATCAAGC 
TTGGAAGATT TAGCAAAACA GCTTTGTGGA GAGGTAATGA TATACCAGCT TGCGGATCAT 
GTGCAGTGTT TTCTTACAGA GTATAACCAT CCACCTCCTA AATCCTTTCA TGAGGAGATG 
TTAAAAAACC TTCAGAAGCA GCAGGAGAAG CTTGCTCTTG AAGAAGAGAG ACGAATGCAA 
GAGATAAAGA TGAAAGAAGA GCAGACGAGA CGGGAAATCC TTAATGAAAT CCAGAAGCGA 
GAAGAAGAGA AAAAGGAAGA AAAAAGAAGG AAGGAAGTAG CAAAACAGGA ACGGATTGAA 
AACGCTAATC TAATGTGCCA AGAGAATGTA AATAAGATTA ATAATACAGG CAAGACAACA 
ACAGCTGCCT CGGATGGGTC ACTTCCTGAT CATGGAGGAA CCAACAGAAA TCGGCCACAT 
TCAGCAGGAC GCTCAAAGCG GGACCGACAG TATTCGGTGT GCCTCACTGA TGACTCAACC 
CCATTTGAAG TGTTAACCTT TGATATTGGA TGCAGTGGGC AAATTGTTGT TCACAGAGGA 
AAGTGCCTGG GGAAAGATGA GCTGCTGGGT AAATCTGTTT ACAATGGCTT GGAAACTGCT 
TCAGGACATT TTGTTGTAAT CTATGAATGG GTTCTCCAGT GGCAGAAAAA GATGGGCAAG 
TTTCTTACTA ATCAAGAAAA AGAAAAGATT GAAAAGTGTA AAAAACAGAT TCAGGGAACA 
GAAACCGAGT TCAGTTCTCT TATCAAACTG AGCCATCCAA ACATTGTGCA CTACCTCGCT 
ATGCGAATAA AAGAGAAAGA AGATTCCATT TCTGTCGGGA TCATAGCTGA ACATGTTAAT 
GGTACAAAGC TTTCCAGTTA CCTTGAGCAG GGTGTACCTG TTCCACTGGA TCAACTACGG 
CACTATACTG CCCAGCTGCT ATCGGCACTG GACTATTTGC ATTCTAATTC TGTGGTACAT 
AAAGTCATGT GCGCCTCCAG TGTTTTGGTG ACAGCTGACG GGAAAGTAAA ACTGACAGAC 
TATAGCCTTT CCAAACGCCT TGCTGACATC TGCAAAGAGG ACGTGTTTGA GCAAACAAAA 
GTTCGATTCA GTGAGGATGC TCTGCCTAGC AAGAGTGGCA AGAAAGGAGA TATATGGAAG 
CTCGGTCTCT TGCTACTTTC GCTGTCACAA GGCAACATAG CCAAAGAGTA TCCTGTAAAA 
GTGCCCAGTG ACCTGCCTGC AGATTTCCAA GATTTCCTGC AAAAGTGTGT TTGCTTGGAA 
GACAAGGAAC GCTGGACTAC ACAGCAGTTA CTAAGTCACA GTTTCCTTAA CCCTCCACCT 
GAGAAGAGTC CTGTAACAGA GGAATGCTCA GATGATTATC CGGGTGACTG TACTGAGACT 
GTCATTCCCA GATCTTCAGT ATCTAATTCC ACATTCCATA TTGAGTCACA GAGACAGTTT 
TCTCGCTACT TCAATGAGTT TGAAGAATTA CAGATGCTTG GGAAAGGGGC ATTTGGTGCT 
GTCATTAAGG TTCAGAACAA ACTGGATGGC TGCTATTACG CTGTAAAACG GGTACAAATC 
AATCCTGCAA GTAAACAGTT CCGCAGAATT AAGGGGGAGG TAACATTGTT ATCTCGGCTC 
AACCATGAAA ACATTGTAAG GTACTATAAT GCATGGATTG AGAAACACGA GCATCCTACT 
ATTTCTCCTA AATGTGAAGC ACATGAGGAA AAGAAAGTGA CTGTGAAGCC AGAACAAATA 
GTAAAGAATG ATTTGGGATT GTTGGATGAT ATTGAGGAAA ACGCACCAGC ACCCATATTA 
ACCAGCTCTG TAGAATGGAG TACATCTTGC GAGCGATCAT CCAGTGCGCA CTGTACTAAT 
GGGGATCAAG ATTCAAGTGA TGATGATGAT GATGACGATG AAGAGGGAGT GTTTTCACCT 
TCGTTCCTGC CTGGGACAGA TTCTGACAGT GAGATCATAT TTGAGAATGA AGATGACATT 
AGTCCAGATC AGGATGAAGA ATATGACAAG AACAATGTAG AAGACCGCAA AGCTGGAGCC 
AATGAGATTG TGACTCAGGC AGTGCATTAT TTATATATTC AGATGGAATA TTGTGAGAAA 
AGCACCTTAC GGGACACGAT AGACCAAGGA CTGTATATGG ATGTTGGCCG CCTCTGGAGG 
CTGTTTCGTG AAATTTTGGA TGGATTGACC TATATACATG AGCAGGGAAT GATCCACAGA 
GACCTAAAGC CAGTCAATAT CTTTTTAGAT TCAGAAGATC ATGTCAAAAT AGGAGATTTT 
GGACTAGCTA CAGATCACCC AGCATCTTAT ACTGAATCAG CACAAGGGGA AGAGGATCAG 
GCGCATTCTG ACTTGGTTAA AGTCGACCCT GAAGATAGGG CCTCAACACC TGGTCGCAAT 
ACTCAAGGAA AACTCACTGG CATGGTTGGC ACTGCCTTGT ATGTAAGCCC TGAAGTCCAA 
GGAAACACCA AAGCTTCATA TAACCAGAAA GTGGACCTTT ACAGCCTGGG TATAATATTG 
TTTGAGATGT CCTACCGGCC CATGGACACA AGTTCTGAAC GTATCTCTGT CTTAAGCAGG 
CTTAGACAGG CCTTGATTGT GTTCCCAAGT GATTTTGAAA ACCCAGAAAA TGAAAAACAG 
AAAAAAGTGA TTACTTGGCT CCTGAACCAT GACCCAGCAT CCCGCCCCAC CGCTATGGAG 
TTGCTTAAGT GCGATCTCTT ACCCCCACCA CAACTAGAGG AGTCTGAACT CCATGAAGTC 
CTGCACCACA CCTTGGCCAA TATCGATGGG AAAGCGTACC GCACAATGAT GAGTCAAATC 
TTTGCCCAGC GCATTTCACC TGCTATGGAT TATACATATG ACAGTGATAT ATTGAAGGGT 
ATTTTCTCAG TCCGTTCTGC CAGACTACAT CATCATGTTT GTGAGGCAGT CTGCAGGATA 
TTTAAAAGAC ACGGGGCTGT GAAGTTACAT GCCCCACTGC TGATGCCACG AGGTAAAGCT 
GGGTTTGATA ACAGTGAATC TGCATGTTTT ATGGATCACA GTGGCATGCT GGTCACCCTT 
CCTTATAACC TCAGGATATC ATTTGCGCGG TTTGTTGCAA GGAATAATAT AACTAGTTTG 
AAAAGGTATA GCATTGAGAG AGTGTTTAGA CCACGCAAAC TGAACCGCTG CCATCCAAAG 
GAACTCACTG AGTGTGCATT TGATATAATC ACTTCTGCTG CAAACTGTTA CCTGCCCATT 
GCAGAAACAA TATACACCAT ATATGAAATT ATCCAGGAAT TTCCAGTACT GCAGGAAAGG 
AATTACAGTA TTTATGTGAA CCACACCTCG TTGTTGAAAG CCATTCTCCT TCACTGTGGC 
GTGCCCGAGG ATAAACTCAC TCAGGTGTAT AACATTCTGT ATGATGCAAT GATTGAGAAG 
CTGACAAAAA GAGAAGTGGA AGCCAAATTT TGTAATCTCT CCCTTTCAGA GAACAGTTTC 
CAATTGAGTC GCCTTTACCG GTTTATTGAG CAGAAAGGAG ACCTCCCAGA TTTGGAGGCA 
ATGATGGTCC CACTCACAAA GCAAAAAACA GGGGTCGGTG TGCTGGCAAA GCAAGGTTTG 
AAAGACTTGG AAGGAGTCAT TGGATTAGTG AAGAGGCTGG GCATTAAATT ACAGATTGTG 
GTTAACTTTG GACTTGTCTA CAAGATGCAG CAGCACAGTG GCATAGTCTT CCAGTTTGTG 
GCTTTTATCA AGCGGAGACA GAGGACTGTG CCTGAAATAC TTGCAGCTGG AGGCAGATAT 
GACCATTTGA TTTCCCAGTT TAGGGGACCT CAGACTGGAG CTATGGGATT ACCCTGTGCT 
GTAGGCGTTA GTATAGCAAT AGACAAGATA TCTGCTGCGC TTTCCACTGC AGAGGAACAT 
ATCAGCAGCT GTGATTTATT AGTCGTAAGC GCTGGGCAGA TGTCTCTGAA TAGAGCCATT 
GGGATAACAC AGAAGCTGTG GGCTGCTGGC ATTACTGCAG AGATCATGTA TGAGTGGTCT 
GAGTCTCAGG ATGAACTGCA GGATTATTGC AAGTGTTCGG GGATTTCGTA TGCTGCAATT 
ATTTCTGACA AAGAAGGAAA TCACATTAAG CTGAAAACAT TTGAGAAGGA AAGACAGAGT 
GAGAAGAGAA TATTGGAATC TGATCTAGTG GATCTCCTGG TGCAGAAACT TCGGATCAGA 
GTCCTTGAAG AAAGAAATAA CAGAGAAACG TCGGACAGTC TCTCAGTGCA AAATGTGAAG 
GGCTCGTTTA ATTCCCAGAG TCAGTTTGAG CCACACAGCG CAATAATTGT GCCAAGTGTC 
AGTGTTATAG CACCAGAAAA GCTATCTGCC AGTGCCAGGC GGAGATTTGA AACCCAGGTG 
CTAACAAAGC TTCAGACACT CATTACAAAC TTCCAGCAGA GAAATGGTGA AATTGAAATT 
TTAGCAGTTG ATCTGCCAAA AGAAACAATA CAGAACTTGT TAGCTTTAGA GTGCGCAGAT 
GAACAAGTCT TCAATGCATC AGTGAAAATG CTAATGTCTC GGCTGCCAAA ACAGAGATAC 
CTGAAATCAG TGTTTGATGA AATATACACC ATTAAAATCG AAAAGCGGGT TCCTGTGCTG 
GTACTATACA GTTATAAAGA TGATTATTAC AAGATCCTTT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012823581.1
CDS	107..5131
Translation	MAGGRGNALLPIELSPQGGLRPTPPACDPPETYSHRQENELEVLESIYSGDFADLRKNSAWKVQQPPEVIIALRPQGNEVHVKVDLWVKCPQNYPDVPPEIKLKNIQGLSNENVNSLTSSLEDLAKQLCGEVMIYQLADHVQCFLTEYNHPPPKSFHEEMLKNLQKQQEKLALEEERRMQEIKMKEEQTRREILNEIQKREEEKKEEKRRKEVAKQERIENANLMCQENVNKINNTGKTTTAASDGSLPDHGGTNRNRPHSAGRSKRDRQYSVCLTDDSTPFEVLTFDIGCSGQIVVHRGKCLGKDELLGKSVYNGLETASGHFVVIYEWVLQWQKKMGKFLTNQEKEKIEKCKKQIQGTETEFSSLIKLSHPNIVHYLAMRIKEKEDSISVGIIAEHVNGTKLSSYLEQGVPVPLDQLRHYTAQLLSALDYLHSNSVVHKVMCASSVLVTADGKVKLTDYSLSKRLADICKEDVFEQTKVRFSEDALPSKSGKKGDIWKLGLLLLSLSQGNIAKEYPVKVPSDLPADFQDFLQKCVCLEDKERWTTQQLLSHSFLNPPPEKSPVTEECSDDYPGDCTETVIPRSSVSNSTFHIESQRQFSRYFNEFEELQMLGKGAFGAVIKVQNKLDGCYYAVKRVQINPASKQFRRIKGEVTLLSRLNHENIVRYYNAWIEKHEHPTISPKCEAHEEKKVTVKPEQIVKNDLGLLDDIEENAPAPILTSSVEWSTSCERSSSAHCTNGDQDSSDDDDDDDEEGVFSPSFLPGTDSDSEIIFENEDDISPDQDEEYDKNNVEDRKAGANEIVTQAVHYLYIQMEYCEKSTLRDTIDQGLYMDVGRLWRLFREILDGLTYIHEQGMIHRDLKPVNIFLDSEDHVKIGDFGLATDHPASYTESAQGEEDQAHSDLVKVDPEDRASTPGRNTQGKLTGMVGTALYVSPEVQGNTKASYNQKVDLYSLGIILFEMSYRPMDTSSERISVLSRLRQALIVFPSDFENPENEKQKKVITWLLNHDPASRPTAMELLKCDLLPPPQLEESELHEVLHHTLANIDGKAYRTMMSQIFAQRISPAMDYTYDSDILKGIFSVRSARLHHHVCEAVCRIFKRHGAVKLHAPLLMPRGKAGFDNSESACFMDHSGMLVTLPYNLRISFARFVARNNITSLKRYSIERVFRPRKLNRCHPKELTECAFDIITSAANCYLPIAETIYTIYEIIQEFPVLQERNYSIYVNHTSLLKAILLHCGVPEDKLTQVYNILYDAMIEKLTKREVEAKFCNLSLSENSFQLSRLYRFIEQKGDLPDLEAMMVPLTKQKTGVGVLAKQGLKDLEGVIGLVKRLGIKLQIVVNFGLVYKMQQHSGIVFQFVAFIKRRQRTVPEILAAGGRYDHLISQFRGPQTGAMGLPCAVGVSIAIDKISAALSTAEEHISSCDLLVVSAGQMSLNRAIGITQKLWAAGITAEIMYEWSESQDELQDYCKCSGISYAAIISDKEGNHIKLKTFEKERQSEKRILESDLVDLLVQKLRIRVLEERNNRETSDSLSVQNVKGSFNSQSQFEPHSAIIVPSVSVIAPEKLSASARRRFETQVLTKLQTLITNFQQRNGEIEILAVDLPKETIQNLLALECADEQVFNASVKMLMSRLPKQRYLKSVFDEIYTIKIEKRVPVLVLYSYKDDYYKILF

Target ORF information:

RefSeq Version	XM_012968127.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis eukaryotic translation initiation factor 2 alpha kinase 4 (eif2ak4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968127.3

ORF Insert Sequence:

ATGGCAGGCG GCCGGGGAAA TGCACTTCTT CCTATTGAGC TCAGCCCGCA AGGGGGTCTG 
CGCCCTACTC CGCCTGCCTG TGACCCCCCT GAAACTTATT CCCACCGGCA GGAGAATGAG 
CTGGAGGTCC TGGAATCCAT CTATAGTGGG GATTTCGCGG ATCTGCGCAA GAACTCTGCC 
TGGAAGGTAC AGCAGCCTCC AGAAGTCATC ATTGCCTTAC GTCCTCAGGG AAATGAGGTT 
CATGTCAAGG TGGATTTATG GGTAAAATGC CCTCAGAACT ATCCTGATGT TCCTCCTGAA 
ATAAAGCTGA AGAACATCCA GGGGTTGTCC AATGAAAATG TGAACTCTCT GACATCAAGC 
TTGGAAGATT TAGCAAAACA GCTTTGTGGA GAGGTAATGA TATACCAGCT TGCGGATCAT 
GTGCAGTGTT TTCTTACAGA GTATAACCAT CCACCTCCTA AATCCTTTCA TGAGGAGATG 
TTAAAAAACC TTCAGAAGCA GCAGGAGAAG CTTGCTCTTG AAGAAGAGAG ACGAATGCAA 
GAGATAAAGA TGAAAGAAGA GCAGACGAGA CGGGAAATCC TTAATGAAAT CCAGAAGCGA 
GAAGAAGAGA AAAAGGAAGA AAAAAGAAGG AAGGAAGTAG CAAAACAGGA ACGGATTGAA 
AACGCTAATC TAATGTGCCA AGAGAATGTA AATAAGATTA ATAATACAGG CAAGACAACA 
ACAGCTGCCT CGGATGGGTC ACTTCCTGAT CATGGAGGAA CCAACAGAAA TCGGCCACAT 
TCAGCAGGAC GCTCAAAGCG GGACCGACAG TATTCGGTGT GCCTCACTGA TGACTCAACC 
CCATTTGAAG TGTTAACCTT TGATATTGGA TGCAGTGGGC AAATTGTTGT TCACAGAGGA 
AAGTGCCTGG GGAAAGATGA GCTGCTGGGT AAATCTGTTT ACAATGGCTT GGAAACTGCT 
TCAGGACATT TTGTTGTAAT CTATGAATGG GTTCTCCAGT GGCAGAAAAA GATGGGCAAG 
TTTCTTACTA ATCAAGAAAA AGAAAAGATT GAAAAGTGTA AAAAACAGAT TCAGGGAACA 
GAAACCGAGT TCAGTTCTCT TATCAAACTG AGCCATCCAA ACATTGTGCA CTACCTCGCT 
ATGCGAATAA AAGAGAAAGA AGATTCCATT TCTGTCGGGA TCATAGCTGA ACATGTTAAT 
GGTACAAAGC TTTCCAGTTA CCTTGAGCAG GGTGTACCTG TTCCACTGGA TCAACTACGG 
CACTATACTG CCCAGCTGCT ATCGGCACTG GACTATTTGC ATTCTAATTC TGTGGTACAT 
AAAGTCATGT GCGCCTCCAG TGTTTTGGTG ACAGCTGACG GGAAAGTAAA ACTGACAGAC 
TATAGCCTTT CCAAACGCCT TGCTGACATC TGCAAAGAGG ACGTGTTTGA GCAAACAAAA 
GTTCGATTCA GTGAGGATGC TCTGCCTAGC AAGAGTGGCA AGAAAGGAGA TATATGGAAG 
CTCGGTCTCT TGCTACTTTC GCTGTCACAA GGCAACATAG CCAAAGAGTA TCCTGTAAAA 
GTGCCCAGTG ACCTGCCTGC AGATTTCCAA GATTTCCTGC AAAAGTGTGT TTGCTTGGAA 
GACAAGGAAC GCTGGACTAC ACAGCAGTTA CTAAGTCACA GTTTCCTTAA CCCTCCACCT 
GAGAAGAGTC CTGTAACAGA GGAATGCTCA GATGATTATC CGGGTGACTG TACTGAGACT 
GTCATTCCCA GATCTTCAGT ATCTAATTCC ACATTCCATA TTGAGTCACA GAGACAGTTT 
TCTCGCTACT TCAATGAGTT TGAAGAATTA CAGATGCTTG GGAAAGGGGC ATTTGGTGCT 
GTCATTAAGG TTCAGAACAA ACTGGATGGC TGCTATTACG CTGTAAAACG GGTACAAATC 
AATCCTGCAA GTAAACAGTT CCGCAGAATT AAGGGGGAGG TAACATTGTT ATCTCGGCTC 
AACCATGAAA ACATTGTAAG GTACTATAAT GCATGGATTG AGAAACACGA GCATCCTACT 
ATTTCTCCTA AATGTGAAGC ACATGAGGAA AAGAAAGTGA CTGTGAAGCC AGAACAAATA 
GTAAAGAATG ATTTGGGATT GTTGGATGAT ATTGAGGAAA ACGCACCAGC ACCCATATTA 
ACCAGCTCTG TAGAATGGAG TACATCTTGC GAGCGATCAT CCAGTGCGCA CTGTACTAAT 
GGGGATCAAG ATTCAAGTGA TGATGATGAT GATGACGATG AAGAGGGAGT GTTTTCACCT 
TCGTTCCTGC CTGGGACAGA TTCTGACAGT GAGATCATAT TTGAGAATGA AGATGACATT 
AGTCCAGATC AGGATGAAGA ATATGACAAG AACAATGTAG AAGACCGCAA AGCTGGAGCC 
AATGAGATTG TGACTCAGGC AGTGCATTAT TTATATATTC AGATGGAATA TTGTGAGAAA 
AGCACCTTAC GGGACACGAT AGACCAAGGA CTGTATATGG ATGTTGGCCG CCTCTGGAGG 
CTGTTTCGTG AAATTTTGGA TGGATTGACC TATATACATG AGCAGGGAAT GATCCACAGA 
GACCTAAAGC CAGTCAATAT CTTTTTAGAT TCAGAAGATC ATGTCAAAAT AGGAGATTTT 
GGACTAGCTA CAGATCACCC AGCATCTTAT ACTGAATCAG CACAAGGGGA AGAGGATCAG 
GCGCATTCTG ACTTGGTTAA AGTCGACCCT GAAGATAGGG CCTCAACACC TGGTCGCAAT 
ACTCAAGGAA AACTCACTGG CATGGTTGGC ACTGCCTTGT ATGTAAGCCC TGAAGTCCAA 
GGAAACACCA AAGCTTCATA TAACCAGAAA GTGGACCTTT ACAGCCTGGG TATAATATTG 
TTTGAGATGT CCTACCGGCC CATGGACACA AGTTCTGAAC GTATCTCTGT CTTAAGCAGG 
CTTAGACAGG CCTTGATTGT GTTCCCAAGT GATTTTGAAA ACCCAGAAAA TGAAAAACAG 
AAAAAAGTGA TTACTTGGCT CCTGAACCAT GACCCAGCAT CCCGCCCCAC CGCTATGGAG 
TTGCTTAAGT GCGATCTCTT ACCCCCACCA CAACTAGAGG AGTCTGAACT CCATGAAGTC 
CTGCACCACA CCTTGGCCAA TATCGATGGG AAAGCGTACC GCACAATGAT GAGTCAAATC 
TTTGCCCAGC GCATTTCACC TGCTATGGAT TATACATATG ACAGTGATAT ATTGAAGGGT 
ATTTTCTCAG TCCGTTCTGC CAGACTACAT CATCATGTTT GTGAGGCAGT CTGCAGGATA 
TTTAAAAGAC ACGGGGCTGT GAAGTTACAT GCCCCACTGC TGATGCCACG AGGTAAAGCT 
GGGTTTGATA ACAGTGAATC TGCATGTTTT ATGGATCACA GTGGCATGCT GGTCACCCTT 
CCTTATAACC TCAGGATATC ATTTGCGCGG TTTGTTGCAA GGAATAATAT AACTAGTTTG 
AAAAGGTATA GCATTGAGAG AGTGTTTAGA CCACGCAAAC TGAACCGCTG CCATCCAAAG 
GAACTCACTG AGTGTGCATT TGATATAATC ACTTCTGCTG CAAACTGTTA CCTGCCCATT 
GCAGAAACAA TATACACCAT ATATGAAATT ATCCAGGAAT TTCCAGTACT GCAGGAAAGG 
AATTACAGTA TTTATGTGAA CCACACCTCG TTGTTGAAAG CCATTCTCCT TCACTGTGGC 
GTGCCCGAGG ATAAACTCAC TCAGGTGTAT AACATTCTGT ATGATGCAAT GATTGAGAAG 
CTGACAAAAA GAGAAGTGGA AGCCAAATTT TGTAATCTCT CCCTTTCAGA GAACAGTTTC 
CAATTGAGTC GCCTTTACCG GTTTATTGAG CAGAAAGGAG ACCTCCCAGA TTTGGAGGCA 
ATGATGGTCC CACTCACAAA GCAAAAAACA GGGGTCGGTG TGCTGGCAAA GCAAGGTTTG 
AAAGACTTGG AAGGAGTCAT TGGATTAGTG AAGAGGCTGG GCATTAAATT ACAGATTGTG 
GTTAACTTTG GACTTGTCTA CAAGATGCAG CAGCACAGTG GCATAGTCTT CCAGTTTGTG 
GCTTTTATCA AGCGGAGACA GAGGACTGTG CCTGAAATAC TTGCAGCTGG AGGCAGATAT 
GACCATTTGA TTTCCCAGTT TAGGGGACCT CAGACTGGAG CTATGGGATT ACCCTGTGCT 
GTAGGCGTTA GTATAGCAAT AGACAAGATA TCTGCTGCGC TTTCCACTGC AGAGGAACAT 
ATCAGCAGCT GTGATTTATT AGTCGTAAGC GCTGGGCAGA TGTCTCTGAA TAGAGCCATT 
GGGATAACAC AGAAGCTGTG GGCTGCTGGC ATTACTGCAG AGATCATGTA TGAGTGGTCT 
GAGTCTCAGG ATGAACTGCA GGATTATTGC AAGTGTTCGG GGATTTCGTA TGCTGCAATT 
ATTTCTGACA AAGAAGGAAA TCACATTAAG CTGAAAACAT TTGAGAAGGA AAGACAGAGT 
GAGAAGAGAA TATTGGAATC TGATCTAGTG GATCTCCTGG TGCAGAAACT TCGGATCAGA 
GTCCTTGAAG AAAGAAATAA CAGAGAAACG TCGGACAGTC TCTCAGTGCA AAATGTGAAG 
GGCTCGTTTA ATTCCCAGAG TCAGTTTGAG CCACACAGCG CAATAATTGT GCCAAGTGTC 
AGTGTTATAG CACCAGAAAA GCTATCTGCC AGTGCCAGGC GGAGATTTGA AACCCAGGTG 
CTAACAAAGC TTCAGACACT CATTACAAAC TTCCAGCAGA GAAATGGTGA AATTGAAATT 
TTAGCAGTTG ATCTGCCAAA AGAAACAATA CAGAACTTGT TAGCTTTAGA GTGCGCAGAT 
GAACAAGTCT TCAATGCATC AGTGAAAATG CTAATGTCTC GGCTGCCAAA ACAGAGATAC 
CTGAAATCAG TGTTTGATGA AATATACACC ATTAAAATCG AAAAGCGGGT TCCTGTGCTG 
GTACTATACA GTTATAAAGA TGATTATTAC AAGATCCTTT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eif2ak4 ( XM_012968128.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823582.1 Xenopus tropicalis eukaryotic translation initiation factor 2 alpha kinase 4 (eif2ak4), transcript variant X2, mRNA.

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CloneID	OXa32795
Clone ID Related Accession (Same CDS sequence)	XM_012968128.3 , XM_012968128.2
Accession Version	XM_012968128.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	eIF-2-alpha kinase GCN2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012968128.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGCG GCCGGGGAAA TGCACTTCTT CCTATTGAGC TCAGCCCGCA AGGGGGTCTG 
CGCCCTACTC CGCCTGCCTG TGACCCCCCT GAAACTTATT CCCACCGGCA GGAGAATGAG 
CTGGAGGTCC TGGAATCCAT CTATAGTGGG GATTTCGCGG ATCTGCGCAA GAACTCTGCC 
TGGAAGGTAC AGCAGCCTCC AGAAGTCATC ATTGCCTTAC GTCCTCAGGG AAATGAGGTT 
CATGTCAAGG TGGATTTATG GGTAAAATGC CCTCAGAACT ATCCTGATGT TCCTCCTGAA 
ATAAAGCTGA AGAACATCCA GGGGTTGTCC AATGAAAATG TGAACTCTCT GACATCAAGC 
TTGGAAGATT TAGCAAAACA GCTTTGTGGA GAGGTAATGA TATACCAGCT TGCGGATCAT 
GTGCAGTGTT TTCTTACAGA GTATAACCAT CCACCTCCTA AATCCTTTCA TGAGGAGATG 
TTAAAAAACC TTCAGAAGCA GCAGGAGAAG CTTGCTCTTG AAGAAGAGAG ACGAATGCAA 
GAGATAAAGA TGAAAGAAGA GCAGACGAGA CGGGAAATCC TTAATGAAAT CCAGAAGCGA 
GAAGAAGAGA AAAAGGAAGA AAAAAGAAGG AAGGAAGTAG CAAAACAGGA ACGGATTGAA 
AACGCTAATC TAATGTGCCA AGAGAATGTA AATAAGATTA ATAATACAGG CAAGACAACA 
ACAGCTGCCT CGGATGGGTC ACTTCCTGAT CATGGAGGAA CCAACAGAAA TCGGCCACAT 
TCAGCAGGAC GCTCAAAGCG GGACCGACAG TATTCGGTGT GCCTCACTGA TGACTCAACC 
CCATTTGAAG TGTTAACCTT TGATATTGGA TGCAGTGGGC AAATTGTTGT TCACAGAGGA 
AAGTGCCTGG GGAAAGATGA GCTGCTGGGT AAATCTGTTT ACAATGGCTT GGAAACTGCT 
TCAGGACATT TTGTTGTAAT CTATGAATGG GTTCTCCAGT GGCAGAAAAA GATGGGCAAG 
TTTCTTACTA ATCAAGAAAA AGAAAAGATT GAAAAGTGTA AAAAACAGAT TCAGGGAACA 
GAAACCGAGT TCAGTTCTCT TATCAAACTG AGCCATCCAA ACATTGTGCA CTACCTCGCT 
ATGCGAATAA AAGAGAAAGA AGATTCCATT TCTGTCGGGA TCATAGCTGA ACATGTTAAT 
GGTACAAAGC TTTCCAGTTA CCTTGAGCAG GGTGTACCTG TTCCACTGGA TCAACTACGG 
CACTATACTG CCCAGCTGCT ATCGGCACTG GACTATTTGC ATTCTAATTC TGTGGTACAT 
AAAGTCATGT GCGCCTCCAG TGTTTTGGTG ACAGCTGACG GGAAAGTAAA ACTGACAGAC 
TATAGCCTTT CCAAACGCCT TGCTGACATC TGCAAAGAGG ACGTGTTTGA GCAAACAAAA 
GTTCGATTCA GTGAGGATGC TCTGCCTAGC AAGAGTGGCA AGAAAGGAGA TATATGGAAG 
CTCGGTCTCT TGCTACTTTC GCTGTCACAA GGCAACATAG CCAAAGAGTA TCCTGTAAAA 
GTGCCCAGTG ACCTGCCTGC AGATTTCCAA GATTTCCTGC AAAAGTGTGT TTGCTTGGAA 
GACAAGGAAC GCTGGACTAC ACAGCAGTTA CTAAGTCACA GTTTCCTTAA CCCTCCACCT 
GAGAAGAGTC CTGTAACAGA GGAATGCTCA GATGATTATC CGGGTGACTG TACTGAGACT 
GTCATTCCCA GATCTTCAGT ATCTAATTCC ACATTCCATA TTGAGTCACA GAGACAGTTT 
TCTCGCTACT TCAATGAGTT TGAAGAATTA CAGATGCTTG GGAAAGGGGC ATTTGGTGCT 
GTCATTAAGG TTCAGAACAA ACTGGATGGC TGCTATTACG CTGTAAAACG GGTACAAATC 
AATCCTGCAA GTAAACAGTT CCGCAGAATT AAGGGGGAGG TAACATTGTT ATCTCGGCTC 
AACCATGAAA ACATTGTAAG GTACTATAAT GCATGGATTG AGAAACACGA GCATCCTACT 
ATTTCTCCTA AATGTGAAGC ACATGAGGAA AAGAAAGTGA CTGTGAAGCC AGAACAAATA 
GTAAAGAATG ATTTGGGATT GTTGGATGAT ATTGAGGAAA ACGCACCAGC ACCCATATTA 
ACCAGCTCTG TAGAATGGAG TACATCTTGC GAGCGATCAT CCAGTGCGCA CTGTACTAAT 
GGGGATCAAG ATTCAAGTGA TGATGATGAT GATGACGATG AAGAGGGAGT GTTTTCACCT 
TCGTTCCTGC CTGGGACAGA TTCTGACAGT GAGATCATAT TTGAGAATGA AGATGACATT 
AGTCCAGATC AGGATGAAGA ATATGACAAG AACAATGTAG AAGACCGCAA AGCTGGAGCC 
AATGAGATTG TGACTCAGGC AGTGCATTAT TTATATATTC AGATGGAATA TTGTGAGAAA 
AGCACCTTAC GGGACACGAT AGACCAAGGA CTGTATATGG ATGTTGGCCG CCTCTGGAGG 
CTGTTTCGTG AAATTTTGGA TGGATTGACC TATATACATG AGCAGGGAAT GATCCACAGA 
GACCTAAAGC CAGTCAATAT CTTTTTAGAT TCAGAAGATC ATGTCAAAAT AGGAGATTTT 
GGACTAGCTA CAGATCACCC AGCATCTTAT ACTGAATCAG CACAAGGGGA AGAGGATCAG 
GCGCATTCTG ACTTGGTTAA AGTCGACCCT GAAGGAAAAC TCACTGGCAT GGTTGGCACT 
GCCTTGTATG TAAGCCCTGA AGTCCAAGGA AACACCAAAG CTTCATATAA CCAGAAAGTG 
GACCTTTACA GCCTGGGTAT AATATTGTTT GAGATGTCCT ACCGGCCCAT GGACACAAGT 
TCTGAACGTA TCTCTGTCTT AAGCAGGCTT AGACAGGCCT TGATTGTGTT CCCAAGTGAT 
TTTGAAAACC CAGAAAATGA AAAACAGAAA AAAGTGATTA CTTGGCTCCT GAACCATGAC 
CCAGCATCCC GCCCCACCGC TATGGAGTTG CTTAAGTGCG ATCTCTTACC CCCACCACAA 
CTAGAGGAGT CTGAACTCCA TGAAGTCCTG CACCACACCT TGGCCAATAT CGATGGGAAA 
GCGTACCGCA CAATGATGAG TCAAATCTTT GCCCAGCGCA TTTCACCTGC TATGGATTAT 
ACATATGACA GTGATATATT GAAGGGTATT TTCTCAGTCC GTTCTGCCAG ACTACATCAT 
CATGTTTGTG AGGCAGTCTG CAGGATATTT AAAAGACACG GGGCTGTGAA GTTACATGCC 
CCACTGCTGA TGCCACGAGG TAAAGCTGGG TTTGATAACA GTGAATCTGC ATGTTTTATG 
GATCACAGTG GCATGCTGGT CACCCTTCCT TATAACCTCA GGATATCATT TGCGCGGTTT 
GTTGCAAGGA ATAATATAAC TAGTTTGAAA AGGTATAGCA TTGAGAGAGT GTTTAGACCA 
CGCAAACTGA ACCGCTGCCA TCCAAAGGAA CTCACTGAGT GTGCATTTGA TATAATCACT 
TCTGCTGCAA ACTGTTACCT GCCCATTGCA GAAACAATAT ACACCATATA TGAAATTATC 
CAGGAATTTC CAGTACTGCA GGAAAGGAAT TACAGTATTT ATGTGAACCA CACCTCGTTG 
TTGAAAGCCA TTCTCCTTCA CTGTGGCGTG CCCGAGGATA AACTCACTCA GGTGTATAAC 
ATTCTGTATG ATGCAATGAT TGAGAAGCTG ACAAAAAGAG AAGTGGAAGC CAAATTTTGT 
AATCTCTCCC TTTCAGAGAA CAGTTTCCAA TTGAGTCGCC TTTACCGGTT TATTGAGCAG 
AAAGGAGACC TCCCAGATTT GGAGGCAATG ATGGTCCCAC TCACAAAGCA AAAAACAGGG 
GTCGGTGTGC TGGCAAAGCA AGGTTTGAAA GACTTGGAAG GAGTCATTGG ATTAGTGAAG 
AGGCTGGGCA TTAAATTACA GATTGTGGTT AACTTTGGAC TTGTCTACAA GATGCAGCAG 
CACAGTGGCA TAGTCTTCCA GTTTGTGGCT TTTATCAAGC GGAGACAGAG GACTGTGCCT 
GAAATACTTG CAGCTGGAGG CAGATATGAC CATTTGATTT CCCAGTTTAG GGGACCTCAG 
ACTGGAGCTA TGGGATTACC CTGTGCTGTA GGCGTTAGTA TAGCAATAGA CAAGATATCT 
GCTGCGCTTT CCACTGCAGA GGAACATATC AGCAGCTGTG ATTTATTAGT CGTAAGCGCT 
GGGCAGATGT CTCTGAATAG AGCCATTGGG ATAACACAGA AGCTGTGGGC TGCTGGCATT 
ACTGCAGAGA TCATGTATGA GTGGTCTGAG TCTCAGGATG AACTGCAGGA TTATTGCAAG 
TGTTCGGGGA TTTCGTATGC TGCAATTATT TCTGACAAAG AAGGAAATCA CATTAAGCTG 
AAAACATTTG AGAAGGAAAG ACAGAGTGAG AAGAGAATAT TGGAATCTGA TCTAGTGGAT 
CTCCTGGTGC AGAAACTTCG GATCAGAGTC CTTGAAGAAA GAAATAACAG AGAAACGTCG 
GACAGTCTCT CAGTGCAAAA TGTGAAGGGC TCGTTTAATT CCCAGAGTCA GTTTGAGCCA 
CACAGCGCAA TAATTGTGCC AAGTGTCAGT GTTATAGCAC CAGAAAAGCT ATCTGCCAGT 
GCCAGGCGGA GATTTGAAAC CCAGGTGCTA ACAAAGCTTC AGACACTCAT TACAAACTTC 
CAGCAGAGAA ATGGTGAAAT TGAAATTTTA GCAGTTGATC TGCCAAAAGA AACAATACAG 
AACTTGTTAG CTTTAGAGTG CGCAGATGAA CAAGTCTTCA ATGCATCAGT GAAAATGCTA 
ATGTCTCGGC TGCCAAAACA GAGATACCTG AAATCAGTGT TTGATGAAAT ATACACCATT 
AAAATCGAAA AGCGGGTTCC TGTGCTGGTA CTATACAGTT ATAAAGATGA TTATTACAAG 
ATCCTTTTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012823582.1
CDS	106..5097
Translation	MAGGRGNALLPIELSPQGGLRPTPPACDPPETYSHRQENELEVLESIYSGDFADLRKNSAWKVQQPPEVIIALRPQGNEVHVKVDLWVKCPQNYPDVPPEIKLKNIQGLSNENVNSLTSSLEDLAKQLCGEVMIYQLADHVQCFLTEYNHPPPKSFHEEMLKNLQKQQEKLALEEERRMQEIKMKEEQTRREILNEIQKREEEKKEEKRRKEVAKQERIENANLMCQENVNKINNTGKTTTAASDGSLPDHGGTNRNRPHSAGRSKRDRQYSVCLTDDSTPFEVLTFDIGCSGQIVVHRGKCLGKDELLGKSVYNGLETASGHFVVIYEWVLQWQKKMGKFLTNQEKEKIEKCKKQIQGTETEFSSLIKLSHPNIVHYLAMRIKEKEDSISVGIIAEHVNGTKLSSYLEQGVPVPLDQLRHYTAQLLSALDYLHSNSVVHKVMCASSVLVTADGKVKLTDYSLSKRLADICKEDVFEQTKVRFSEDALPSKSGKKGDIWKLGLLLLSLSQGNIAKEYPVKVPSDLPADFQDFLQKCVCLEDKERWTTQQLLSHSFLNPPPEKSPVTEECSDDYPGDCTETVIPRSSVSNSTFHIESQRQFSRYFNEFEELQMLGKGAFGAVIKVQNKLDGCYYAVKRVQINPASKQFRRIKGEVTLLSRLNHENIVRYYNAWIEKHEHPTISPKCEAHEEKKVTVKPEQIVKNDLGLLDDIEENAPAPILTSSVEWSTSCERSSSAHCTNGDQDSSDDDDDDDEEGVFSPSFLPGTDSDSEIIFENEDDISPDQDEEYDKNNVEDRKAGANEIVTQAVHYLYIQMEYCEKSTLRDTIDQGLYMDVGRLWRLFREILDGLTYIHEQGMIHRDLKPVNIFLDSEDHVKIGDFGLATDHPASYTESAQGEEDQAHSDLVKVDPEGKLTGMVGTALYVSPEVQGNTKASYNQKVDLYSLGIILFEMSYRPMDTSSERISVLSRLRQALIVFPSDFENPENEKQKKVITWLLNHDPASRPTAMELLKCDLLPPPQLEESELHEVLHHTLANIDGKAYRTMMSQIFAQRISPAMDYTYDSDILKGIFSVRSARLHHHVCEAVCRIFKRHGAVKLHAPLLMPRGKAGFDNSESACFMDHSGMLVTLPYNLRISFARFVARNNITSLKRYSIERVFRPRKLNRCHPKELTECAFDIITSAANCYLPIAETIYTIYEIIQEFPVLQERNYSIYVNHTSLLKAILLHCGVPEDKLTQVYNILYDAMIEKLTKREVEAKFCNLSLSENSFQLSRLYRFIEQKGDLPDLEAMMVPLTKQKTGVGVLAKQGLKDLEGVIGLVKRLGIKLQIVVNFGLVYKMQQHSGIVFQFVAFIKRRQRTVPEILAAGGRYDHLISQFRGPQTGAMGLPCAVGVSIAIDKISAALSTAEEHISSCDLLVVSAGQMSLNRAIGITQKLWAAGITAEIMYEWSESQDELQDYCKCSGISYAAIISDKEGNHIKLKTFEKERQSEKRILESDLVDLLVQKLRIRVLEERNNRETSDSLSVQNVKGSFNSQSQFEPHSAIIVPSVSVIAPEKLSASARRRFETQVLTKLQTLITNFQQRNGEIEILAVDLPKETIQNLLALECADEQVFNASVKMLMSRLPKQRYLKSVFDEIYTIKIEKRVPVLVLYSYKDDYYKILF

Target ORF information:

RefSeq Version	XM_012968128.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis eukaryotic translation initiation factor 2 alpha kinase 4 (eif2ak4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968128.3

ORF Insert Sequence:

ATGGCAGGCG GCCGGGGAAA TGCACTTCTT CCTATTGAGC TCAGCCCGCA AGGGGGTCTG 
CGCCCTACTC CGCCTGCCTG TGACCCCCCT GAAACTTATT CCCACCGGCA GGAGAATGAG 
CTGGAGGTCC TGGAATCCAT CTATAGTGGG GATTTCGCGG ATCTGCGCAA GAACTCTGCC 
TGGAAGGTAC AGCAGCCTCC AGAAGTCATC ATTGCCTTAC GTCCTCAGGG AAATGAGGTT 
CATGTCAAGG TGGATTTATG GGTAAAATGC CCTCAGAACT ATCCTGATGT TCCTCCTGAA 
ATAAAGCTGA AGAACATCCA GGGGTTGTCC AATGAAAATG TGAACTCTCT GACATCAAGC 
TTGGAAGATT TAGCAAAACA GCTTTGTGGA GAGGTAATGA TATACCAGCT TGCGGATCAT 
GTGCAGTGTT TTCTTACAGA GTATAACCAT CCACCTCCTA AATCCTTTCA TGAGGAGATG 
TTAAAAAACC TTCAGAAGCA GCAGGAGAAG CTTGCTCTTG AAGAAGAGAG ACGAATGCAA 
GAGATAAAGA TGAAAGAAGA GCAGACGAGA CGGGAAATCC TTAATGAAAT CCAGAAGCGA 
GAAGAAGAGA AAAAGGAAGA AAAAAGAAGG AAGGAAGTAG CAAAACAGGA ACGGATTGAA 
AACGCTAATC TAATGTGCCA AGAGAATGTA AATAAGATTA ATAATACAGG CAAGACAACA 
ACAGCTGCCT CGGATGGGTC ACTTCCTGAT CATGGAGGAA CCAACAGAAA TCGGCCACAT 
TCAGCAGGAC GCTCAAAGCG GGACCGACAG TATTCGGTGT GCCTCACTGA TGACTCAACC 
CCATTTGAAG TGTTAACCTT TGATATTGGA TGCAGTGGGC AAATTGTTGT TCACAGAGGA 
AAGTGCCTGG GGAAAGATGA GCTGCTGGGT AAATCTGTTT ACAATGGCTT GGAAACTGCT 
TCAGGACATT TTGTTGTAAT CTATGAATGG GTTCTCCAGT GGCAGAAAAA GATGGGCAAG 
TTTCTTACTA ATCAAGAAAA AGAAAAGATT GAAAAGTGTA AAAAACAGAT TCAGGGAACA 
GAAACCGAGT TCAGTTCTCT TATCAAACTG AGCCATCCAA ACATTGTGCA CTACCTCGCT 
ATGCGAATAA AAGAGAAAGA AGATTCCATT TCTGTCGGGA TCATAGCTGA ACATGTTAAT 
GGTACAAAGC TTTCCAGTTA CCTTGAGCAG GGTGTACCTG TTCCACTGGA TCAACTACGG 
CACTATACTG CCCAGCTGCT ATCGGCACTG GACTATTTGC ATTCTAATTC TGTGGTACAT 
AAAGTCATGT GCGCCTCCAG TGTTTTGGTG ACAGCTGACG GGAAAGTAAA ACTGACAGAC 
TATAGCCTTT CCAAACGCCT TGCTGACATC TGCAAAGAGG ACGTGTTTGA GCAAACAAAA 
GTTCGATTCA GTGAGGATGC TCTGCCTAGC AAGAGTGGCA AGAAAGGAGA TATATGGAAG 
CTCGGTCTCT TGCTACTTTC GCTGTCACAA GGCAACATAG CCAAAGAGTA TCCTGTAAAA 
GTGCCCAGTG ACCTGCCTGC AGATTTCCAA GATTTCCTGC AAAAGTGTGT TTGCTTGGAA 
GACAAGGAAC GCTGGACTAC ACAGCAGTTA CTAAGTCACA GTTTCCTTAA CCCTCCACCT 
GAGAAGAGTC CTGTAACAGA GGAATGCTCA GATGATTATC CGGGTGACTG TACTGAGACT 
GTCATTCCCA GATCTTCAGT ATCTAATTCC ACATTCCATA TTGAGTCACA GAGACAGTTT 
TCTCGCTACT TCAATGAGTT TGAAGAATTA CAGATGCTTG GGAAAGGGGC ATTTGGTGCT 
GTCATTAAGG TTCAGAACAA ACTGGATGGC TGCTATTACG CTGTAAAACG GGTACAAATC 
AATCCTGCAA GTAAACAGTT CCGCAGAATT AAGGGGGAGG TAACATTGTT ATCTCGGCTC 
AACCATGAAA ACATTGTAAG GTACTATAAT GCATGGATTG AGAAACACGA GCATCCTACT 
ATTTCTCCTA AATGTGAAGC ACATGAGGAA AAGAAAGTGA CTGTGAAGCC AGAACAAATA 
GTAAAGAATG ATTTGGGATT GTTGGATGAT ATTGAGGAAA ACGCACCAGC ACCCATATTA 
ACCAGCTCTG TAGAATGGAG TACATCTTGC GAGCGATCAT CCAGTGCGCA CTGTACTAAT 
GGGGATCAAG ATTCAAGTGA TGATGATGAT GATGACGATG AAGAGGGAGT GTTTTCACCT 
TCGTTCCTGC CTGGGACAGA TTCTGACAGT GAGATCATAT TTGAGAATGA AGATGACATT 
AGTCCAGATC AGGATGAAGA ATATGACAAG AACAATGTAG AAGACCGCAA AGCTGGAGCC 
AATGAGATTG TGACTCAGGC AGTGCATTAT TTATATATTC AGATGGAATA TTGTGAGAAA 
AGCACCTTAC GGGACACGAT AGACCAAGGA CTGTATATGG ATGTTGGCCG CCTCTGGAGG 
CTGTTTCGTG AAATTTTGGA TGGATTGACC TATATACATG AGCAGGGAAT GATCCACAGA 
GACCTAAAGC CAGTCAATAT CTTTTTAGAT TCAGAAGATC ATGTCAAAAT AGGAGATTTT 
GGACTAGCTA CAGATCACCC AGCATCTTAT ACTGAATCAG CACAAGGGGA AGAGGATCAG 
GCGCATTCTG ACTTGGTTAA AGTCGACCCT GAAGGAAAAC TCACTGGCAT GGTTGGCACT 
GCCTTGTATG TAAGCCCTGA AGTCCAAGGA AACACCAAAG CTTCATATAA CCAGAAAGTG 
GACCTTTACA GCCTGGGTAT AATATTGTTT GAGATGTCCT ACCGGCCCAT GGACACAAGT 
TCTGAACGTA TCTCTGTCTT AAGCAGGCTT AGACAGGCCT TGATTGTGTT CCCAAGTGAT 
TTTGAAAACC CAGAAAATGA AAAACAGAAA AAAGTGATTA CTTGGCTCCT GAACCATGAC 
CCAGCATCCC GCCCCACCGC TATGGAGTTG CTTAAGTGCG ATCTCTTACC CCCACCACAA 
CTAGAGGAGT CTGAACTCCA TGAAGTCCTG CACCACACCT TGGCCAATAT CGATGGGAAA 
GCGTACCGCA CAATGATGAG TCAAATCTTT GCCCAGCGCA TTTCACCTGC TATGGATTAT 
ACATATGACA GTGATATATT GAAGGGTATT TTCTCAGTCC GTTCTGCCAG ACTACATCAT 
CATGTTTGTG AGGCAGTCTG CAGGATATTT AAAAGACACG GGGCTGTGAA GTTACATGCC 
CCACTGCTGA TGCCACGAGG TAAAGCTGGG TTTGATAACA GTGAATCTGC ATGTTTTATG 
GATCACAGTG GCATGCTGGT CACCCTTCCT TATAACCTCA GGATATCATT TGCGCGGTTT 
GTTGCAAGGA ATAATATAAC TAGTTTGAAA AGGTATAGCA TTGAGAGAGT GTTTAGACCA 
CGCAAACTGA ACCGCTGCCA TCCAAAGGAA CTCACTGAGT GTGCATTTGA TATAATCACT 
TCTGCTGCAA ACTGTTACCT GCCCATTGCA GAAACAATAT ACACCATATA TGAAATTATC 
CAGGAATTTC CAGTACTGCA GGAAAGGAAT TACAGTATTT ATGTGAACCA CACCTCGTTG 
TTGAAAGCCA TTCTCCTTCA CTGTGGCGTG CCCGAGGATA AACTCACTCA GGTGTATAAC 
ATTCTGTATG ATGCAATGAT TGAGAAGCTG ACAAAAAGAG AAGTGGAAGC CAAATTTTGT 
AATCTCTCCC TTTCAGAGAA CAGTTTCCAA TTGAGTCGCC TTTACCGGTT TATTGAGCAG 
AAAGGAGACC TCCCAGATTT GGAGGCAATG ATGGTCCCAC TCACAAAGCA AAAAACAGGG 
GTCGGTGTGC TGGCAAAGCA AGGTTTGAAA GACTTGGAAG GAGTCATTGG ATTAGTGAAG 
AGGCTGGGCA TTAAATTACA GATTGTGGTT AACTTTGGAC TTGTCTACAA GATGCAGCAG 
CACAGTGGCA TAGTCTTCCA GTTTGTGGCT TTTATCAAGC GGAGACAGAG GACTGTGCCT 
GAAATACTTG CAGCTGGAGG CAGATATGAC CATTTGATTT CCCAGTTTAG GGGACCTCAG 
ACTGGAGCTA TGGGATTACC CTGTGCTGTA GGCGTTAGTA TAGCAATAGA CAAGATATCT 
GCTGCGCTTT CCACTGCAGA GGAACATATC AGCAGCTGTG ATTTATTAGT CGTAAGCGCT 
GGGCAGATGT CTCTGAATAG AGCCATTGGG ATAACACAGA AGCTGTGGGC TGCTGGCATT 
ACTGCAGAGA TCATGTATGA GTGGTCTGAG TCTCAGGATG AACTGCAGGA TTATTGCAAG 
TGTTCGGGGA TTTCGTATGC TGCAATTATT TCTGACAAAG AAGGAAATCA CATTAAGCTG 
AAAACATTTG AGAAGGAAAG ACAGAGTGAG AAGAGAATAT TGGAATCTGA TCTAGTGGAT 
CTCCTGGTGC AGAAACTTCG GATCAGAGTC CTTGAAGAAA GAAATAACAG AGAAACGTCG 
GACAGTCTCT CAGTGCAAAA TGTGAAGGGC TCGTTTAATT CCCAGAGTCA GTTTGAGCCA 
CACAGCGCAA TAATTGTGCC AAGTGTCAGT GTTATAGCAC CAGAAAAGCT ATCTGCCAGT 
GCCAGGCGGA GATTTGAAAC CCAGGTGCTA ACAAAGCTTC AGACACTCAT TACAAACTTC 
CAGCAGAGAA ATGGTGAAAT TGAAATTTTA GCAGTTGATC TGCCAAAAGA AACAATACAG 
AACTTGTTAG CTTTAGAGTG CGCAGATGAA CAAGTCTTCA ATGCATCAGT GAAAATGCTA 
ATGTCTCGGC TGCCAAAACA GAGATACCTG AAATCAGTGT TTGATGAAAT ATACACCATT 
AAAATCGAAA AGCGGGTTCC TGTGCTGGTA CTATACAGTT ATAAAGATGA TTATTACAAG 
ATCCTTTTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eif2ak4 ( XM_018096178.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951667.1 Xenopus tropicalis eukaryotic translation initiation factor 2 alpha kinase 4 (eif2ak4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa45985
Clone ID Related Accession (Same CDS sequence)	XM_018096178.1
Accession Version	XM_018096178.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	eIF-2-alpha kinase GCN2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGCG GCCGGGGAAA TGCACTTCTT CCTATTGAGC TCAGCCCGCA AGGGGGTCTG 
CGCCCTACTC CGCCTGCCTG TGACCCCCCT GAAACTTATT CCCACCGGCA GGAGAATGAG 
CTGGAGGTCC TGGAATCCAT CTATAGTGGG GATTTCGCGG ATCTGCGCAA GAACTCTGCC 
TGGAAGGTAC AGCAGCCTCC AGAAGTCATC ATTGCCTTAC GTCCTCAGGG AAATGAGGTT 
CATGTCAAGG TGGATTTATG GGTAAAATGC CCTCAGAACT ATCCTGATGT TCCTCCTGAA 
ATAAAGCTGA AGAACATCCA GGGGTTGTCC AATGAAAATG TGAACTCTCT GACATCAAGC 
TTGGAAGATT TAGCAAAACA GCTTTGTGGA GAGGTAATGA TATACCAGCT TGCGGATCAT 
GTGCAGTGTT TTCTTACAGA GTATAACCAT CCACCTCCTA AATCCTTTCA TGAGGAGATG 
TTAAAAAACC TTCAGAAGCA GCAGGAGAAG CTTGCTCTTG AAGAAGAGAG ACGAATGCAA 
GAGATAAAGA TGAAAGAAGA GCAGACGAGA CGGGAAATCC TTAATGAAAT CCAGAAGCGA 
GAAGAAGAGA AAAAGGAAGA AAAAAGAAGG AAGGAAGTAG CAAAACAGGA ACGGATTGAA 
AACGCTAATC TAATGTGCCA AGAGAATGTA AATAAGATTA ATAATACAGG CAAGACAACA 
ACAGCTGCCT CGGATGGGTC ACTTCCTGAT CATGGAGGAA CCAACAGAAA TCGGCCACAT 
TCAGCAGGAC GCTCAAAGCG GGACCGACAG TATTCGGTGT GCCTCACTGA TGACTCAACC 
CCATTTGAAG TGTTAACCTT TGATATTGGA TGCAGTGGGC AAATTGTTGT TCACAGAGGA 
AAGTGCCTGG GGAAAGATGA GCTGCTGGGT AAATCTGTTT ACAATGGCTT GGAAACTGCT 
TCAGGACATT TTGTTGTAAT CTATGAATGG GTTCTCCAGT GGCAGAAAAA GATGGGCAAG 
TTTCTTACTA ATCAAGAAAA AGAAAAGATT GAAAAGTGTA AAAAACAGAT TCAGGGAACA 
GAAACCGAGT TCAGTTCTCT TATCAAACTG AGCCATCCAA ACATTGTGCA CTACCTCGCT 
ATGCGAATAA AAGAGAAAGA AGATTCCATT TCTGTCGGGA TCATAGCTGA ACATGTTAAT 
GGTACAAAGC TTTCCAGTTA CCTTGAGCAG GGTGTACCTG TTCCACTGGA TCAACTACGG 
CACTATACTG CCCAGCTGCT ATCGGCACTG GACTATTTGC ATTCTAATTC TGTGGTACAT 
AAAGTCATGT GCGCCTCCAG TGTTTTGGTG ACAGCTGACG GGAAAGTAAA ACTGACAGAC 
TATAGCCTTT CCAAACGCCT TGCTGACATC TGCAAAGAGG ACGTGTTTGA GCAAACAAAA 
GTTCGATTCA GTGAGGATGC TCTGCCTAGC AAGAGTGGCA AGAAAGGAGA TATATGGAAG 
CTCGGTCTCT TGCTACTTTC GCTGTCACAA GGCAACATAG CCAAAGAGTA TCCTGTAAAA 
GTGCCCAGTG ACCTGCCTGC AGATTTCCAA GATTTCCTGC AAAAGTGTGT TTGCTTGGAA 
GACAAGGAAC GCTGGACTAC ACAGCAGTTA CTAAGTCACA GTTTCCTTAA CCCTCCACCT 
GAGAAGAGTC CTGTAACAGA GGAATGCTCA GATGATTATC CGGGTGACTG TACTGAGACT 
GTCATTCCCA GATCTTCAGT ATCTAATTCC ACATTCCATA TTGAGTCACA GAGACAGTTT 
TCTCGCTACT TCAATGAGTT TGAAGAATTA CAGATGCTTG GGAAAGGGGC ATTTGGTGCT 
GTCATTAAGG TTCAGAACAA ACTGGATGGC TGCTATTACG CTGTAAAACG GGTACAAATC 
AATCCTGCAA GTAAACAGTT CCGCAGAATT AAGGGGGAGG TAACATTGTT ATCTCGGCTC 
AACCATGAAA ACATTGTAAG GTACTATAAT GCATGGATTG AGAAACACGA GCATCCTACT 
ATTTCTCCTA AATGTGAAGC ACATGAGGAA AAGAAAGTGA CTGTGAAGCC AGAACAAATA 
GTAAAGAATG ATTTGGGATT GTTGGATGAT ATTGAGGAAA ACGCACCAGC ACCCATATTA 
ACCAGCTCTG TAGAATGGAG TACATCTTGC GAGCGATCAT CCAGTGCGCA CTGTACTAAT 
GGGGATCAAG ATTCAAGTGA TGATGATGAT GATGACGATG AAGAGGGAGT GTTTTCACCT 
TCGTTCCTGC CTGGGACAGA TTCTGACAGT GAGATCATAT TTGAGAATGA AGATGACATT 
AGTCCAGATC AGGATGAAGA ATATGACAAG AACAATGTAG AAGACCGCAA AGCTGGAGCC 
AATGAGATTG TGACTCAGGC AGTGCATTAT TTATATATTC AGATGGAATA TTGTGAGAAA 
AGCACCTTAC GGGACACGAT AGACCAAGGA CTGTATATGG ATGTTGGCCG CCTCTGGAGG 
CTGTTTCGTG AAATTTTGGA TGGATTGACC TATATACATG AGCAGGGAAT GATCCACAGA 
GACCTAAAGC CAGTCAATAT CTTTTTAGAT TCAGAAGATC ATGTCAAAAT AGGAGATTTT 
GGACTAGCTA CAGATCACCC AGCATCTTAT ACTGAATCAG CACAAGGGGA AGAGGATCAG 
GCGCATTCTG ACTTGGTTAA AGTCGACCCT GAAGATAGGG CCTCAACACC TGGTCGCAAT 
ACTCAAGGAA AACTCACTGG CATGGTTGGC ACTGCCTTGT ATGTAAGCCC TGAAGTCCAA 
GGAAACACCA AAGCTTCATA TAACCAGAAA GTGGACCTTT ACAGCCTGGG TATAATATTG 
TTTGAGATGT CCTACCGGCC CATGGACACA AGTTCTGAAC GTATCTCTGT CTTAAGCAGG 
CTTAGACAGA AAAAAGTGAT TACTTGGCTC CTGAACCATG ACCCAGCATC CCGCCCCACC 
GCTATGGAGT TGCTTAAGTG CGATCTCTTA CCCCCACCAC AACTAGAGGA GTCTGAACTC 
CATGAAGTCC TGCACCACAC CTTGGCCAAT ATCGATGGGA AAGCGTACCG CACAATGATG 
AGTCAAATCT TTGCCCAGCG CATTTCACCT GCTATGGATT ATACATATGA CAGTGATATA 
TTGAAGGGTA TTTTCTCAGT CCGTTCTGCC AGACTACATC ATCATGTTTG TGAGGCAGTC 
TGCAGGATAT TTAAAAGACA CGGGGCTGTG AAGTTACATG CCCCACTGCT GATGCCACGA 
GGTAAAGCTG GGTTTGATAA CAGTGAATCT GCATGTTTTA TGGATCACAG TGGCATGCTG 
GTCACCCTTC CTTATAACCT CAGGATATCA TTTGCGCGGT TTGTTGCAAG GAATAATATA 
ACTAGTTTGA AAAGGTATAG CATTGAGAGA GTGTTTAGAC CACGCAAACT GAACCGCTGC 
CATCCAAAGG AACTCACTGA GTGTGCATTT GATATAATCA CTTCTGCTGC AAACTGTTAC 
CTGCCCATTG CAGAAACAAT ATACACCATA TATGAAATTA TCCAGGAATT TCCAGTACTG 
CAGGAAAGGA ATTACAGTAT TTATGTGAAC CACACCTCGT TGTTGAAAGC CATTCTCCTT 
CACTGTGGCG TGCCCGAGGA TAAACTCACT CAGGTGTATA ACATTCTGTA TGATGCAATG 
ATTGAGAAGC TGACAAAAAG AGAAGTGGAA GCCAAATTTT GTAATCTCTC CCTTTCAGAG 
AACAGTTTCC AATTGAGTCG CCTTTACCGG TTTATTGAGC AGAAAGGAGA CCTCCCAGAT 
TTGGAGGCAA TGATGGTCCC ACTCACAAAG CAAAAAACAG GGGTCGGTGT GCTGGCAAAG 
CAAGGTTTGA AAGACTTGGA AGGAGTCATT GGATTAGTGA AGAGGCTGGG CATTAAATTA 
CAGATTGTGG TTAACTTTGG ACTTGTCTAC AAGATGCAGC AGCACAGTGG CATAGTCTTC 
CAGTTTGTGG CTTTTATCAA GCGGAGACAG AGGACTGTGC CTGAAATACT TGCAGCTGGA 
GGCAGATATG ACCATTTGAT TTCCCAGTTT AGGGGACCTC AGACTGGAGC TATGGGATTA 
CCCTGTGCTG TAGGCGTTAG TATAGCAATA GACAAGATAT CTGCTGCGCT TTCCACTGCA 
GAGGAACATA TCAGCAGCTG TGATTTATTA GTCGTAAGCG CTGGGCAGAT GTCTCTGAAT 
AGAGCCATTG GGATAACACA GAAGCTGTGG GCTGCTGGCA TTACTGCAGA GATCATGTAT 
GAGTGGTCTG AGTCTCAGGA TGAACTGCAG GATTATTGCA AGTGTTCGGG GATTTCGTAT 
GCTGCAATTA TTTCTGACAA AGAAGGAAAT CACATTAAGC TGAAAACATT TGAGAAGGAA 
AGACAGAGTG AGAAGAGAAT ATTGGAATCT GATCTAGTGG ATCTCCTGGT GCAGAAACTT 
CGGATCAGAG TCCTTGAAGA AAGAAATAAC AGAGAAACGT CGGACAGTCT CTCAGTGCAA 
AATGTGAAGG GCTCGTTTAA TTCCCAGAGT CAGTTTGAGC CACACAGCGC AATAATTGTG 
CCAAGTGTCA GTGTTATAGC ACCAGAAAAG CTATCTGCCA GTGCCAGGCG GAGATTTGAA 
ACCCAGGTGC TAACAAAGCT TCAGACACTC ATTACAAACT TCCAGCAGAG AAATGGTGAA 
ATTGAAATTT TAGCAGTTGA TCTGCCAAAA GAAACAATAC AGAACTTGTT AGCTTTAGAG 
TGCGCAGATG AACAAGTCTT CAATGCATCA GTGAAAATGC TAATGTCTCG GCTGCCAAAA 
CAGAGATACC TGAAATCAGT GTTTGATGAA ATATACACCA TTAAAATCGA AAAGCGGGTT 
CCTGTGCTGG TACTATACAG TTATAAAGAT GATTATTACA AGATCCTTTT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951667.1
CDS	648..5621
Translation	MAGGRGNALLPIELSPQGGLRPTPPACDPPETYSHRQENELEVLESIYSGDFADLRKNSAWKVQQPPEVIIALRPQGNEVHVKVDLWVKCPQNYPDVPPEIKLKNIQGLSNENVNSLTSSLEDLAKQLCGEVMIYQLADHVQCFLTEYNHPPPKSFHEEMLKNLQKQQEKLALEEERRMQEIKMKEEQTRREILNEIQKREEEKKEEKRRKEVAKQERIENANLMCQENVNKINNTGKTTTAASDGSLPDHGGTNRNRPHSAGRSKRDRQYSVCLTDDSTPFEVLTFDIGCSGQIVVHRGKCLGKDELLGKSVYNGLETASGHFVVIYEWVLQWQKKMGKFLTNQEKEKIEKCKKQIQGTETEFSSLIKLSHPNIVHYLAMRIKEKEDSISVGIIAEHVNGTKLSSYLEQGVPVPLDQLRHYTAQLLSALDYLHSNSVVHKVMCASSVLVTADGKVKLTDYSLSKRLADICKEDVFEQTKVRFSEDALPSKSGKKGDIWKLGLLLLSLSQGNIAKEYPVKVPSDLPADFQDFLQKCVCLEDKERWTTQQLLSHSFLNPPPEKSPVTEECSDDYPGDCTETVIPRSSVSNSTFHIESQRQFSRYFNEFEELQMLGKGAFGAVIKVQNKLDGCYYAVKRVQINPASKQFRRIKGEVTLLSRLNHENIVRYYNAWIEKHEHPTISPKCEAHEEKKVTVKPEQIVKNDLGLLDDIEENAPAPILTSSVEWSTSCERSSSAHCTNGDQDSSDDDDDDDEEGVFSPSFLPGTDSDSEIIFENEDDISPDQDEEYDKNNVEDRKAGANEIVTQAVHYLYIQMEYCEKSTLRDTIDQGLYMDVGRLWRLFREILDGLTYIHEQGMIHRDLKPVNIFLDSEDHVKIGDFGLATDHPASYTESAQGEEDQAHSDLVKVDPEDRASTPGRNTQGKLTGMVGTALYVSPEVQGNTKASYNQKVDLYSLGIILFEMSYRPMDTSSERISVLSRLRQKKVITWLLNHDPASRPTAMELLKCDLLPPPQLEESELHEVLHHTLANIDGKAYRTMMSQIFAQRISPAMDYTYDSDILKGIFSVRSARLHHHVCEAVCRIFKRHGAVKLHAPLLMPRGKAGFDNSESACFMDHSGMLVTLPYNLRISFARFVARNNITSLKRYSIERVFRPRKLNRCHPKELTECAFDIITSAANCYLPIAETIYTIYEIIQEFPVLQERNYSIYVNHTSLLKAILLHCGVPEDKLTQVYNILYDAMIEKLTKREVEAKFCNLSLSENSFQLSRLYRFIEQKGDLPDLEAMMVPLTKQKTGVGVLAKQGLKDLEGVIGLVKRLGIKLQIVVNFGLVYKMQQHSGIVFQFVAFIKRRQRTVPEILAAGGRYDHLISQFRGPQTGAMGLPCAVGVSIAIDKISAALSTAEEHISSCDLLVVSAGQMSLNRAIGITQKLWAAGITAEIMYEWSESQDELQDYCKCSGISYAAIISDKEGNHIKLKTFEKERQSEKRILESDLVDLLVQKLRIRVLEERNNRETSDSLSVQNVKGSFNSQSQFEPHSAIIVPSVSVIAPEKLSASARRRFETQVLTKLQTLITNFQQRNGEIEILAVDLPKETIQNLLALECADEQVFNASVKMLMSRLPKQRYLKSVFDEIYTIKIEKRVPVLVLYSYKDDYYKILF

Target ORF information:

RefSeq Version	XM_018096178.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis eukaryotic translation initiation factor 2 alpha kinase 4 (eif2ak4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096178.1

ORF Insert Sequence:

ATGGCAGGCG GCCGGGGAAA TGCACTTCTT CCTATTGAGC TCAGCCCGCA AGGGGGTCTG 
CGCCCTACTC CGCCTGCCTG TGACCCCCCT GAAACTTATT CCCACCGGCA GGAGAATGAG 
CTGGAGGTCC TGGAATCCAT CTATAGTGGG GATTTCGCGG ATCTGCGCAA GAACTCTGCC 
TGGAAGGTAC AGCAGCCTCC AGAAGTCATC ATTGCCTTAC GTCCTCAGGG AAATGAGGTT 
CATGTCAAGG TGGATTTATG GGTAAAATGC CCTCAGAACT ATCCTGATGT TCCTCCTGAA 
ATAAAGCTGA AGAACATCCA GGGGTTGTCC AATGAAAATG TGAACTCTCT GACATCAAGC 
TTGGAAGATT TAGCAAAACA GCTTTGTGGA GAGGTAATGA TATACCAGCT TGCGGATCAT 
GTGCAGTGTT TTCTTACAGA GTATAACCAT CCACCTCCTA AATCCTTTCA TGAGGAGATG 
TTAAAAAACC TTCAGAAGCA GCAGGAGAAG CTTGCTCTTG AAGAAGAGAG ACGAATGCAA 
GAGATAAAGA TGAAAGAAGA GCAGACGAGA CGGGAAATCC TTAATGAAAT CCAGAAGCGA 
GAAGAAGAGA AAAAGGAAGA AAAAAGAAGG AAGGAAGTAG CAAAACAGGA ACGGATTGAA 
AACGCTAATC TAATGTGCCA AGAGAATGTA AATAAGATTA ATAATACAGG CAAGACAACA 
ACAGCTGCCT CGGATGGGTC ACTTCCTGAT CATGGAGGAA CCAACAGAAA TCGGCCACAT 
TCAGCAGGAC GCTCAAAGCG GGACCGACAG TATTCGGTGT GCCTCACTGA TGACTCAACC 
CCATTTGAAG TGTTAACCTT TGATATTGGA TGCAGTGGGC AAATTGTTGT TCACAGAGGA 
AAGTGCCTGG GGAAAGATGA GCTGCTGGGT AAATCTGTTT ACAATGGCTT GGAAACTGCT 
TCAGGACATT TTGTTGTAAT CTATGAATGG GTTCTCCAGT GGCAGAAAAA GATGGGCAAG 
TTTCTTACTA ATCAAGAAAA AGAAAAGATT GAAAAGTGTA AAAAACAGAT TCAGGGAACA 
GAAACCGAGT TCAGTTCTCT TATCAAACTG AGCCATCCAA ACATTGTGCA CTACCTCGCT 
ATGCGAATAA AAGAGAAAGA AGATTCCATT TCTGTCGGGA TCATAGCTGA ACATGTTAAT 
GGTACAAAGC TTTCCAGTTA CCTTGAGCAG GGTGTACCTG TTCCACTGGA TCAACTACGG 
CACTATACTG CCCAGCTGCT ATCGGCACTG GACTATTTGC ATTCTAATTC TGTGGTACAT 
AAAGTCATGT GCGCCTCCAG TGTTTTGGTG ACAGCTGACG GGAAAGTAAA ACTGACAGAC 
TATAGCCTTT CCAAACGCCT TGCTGACATC TGCAAAGAGG ACGTGTTTGA GCAAACAAAA 
GTTCGATTCA GTGAGGATGC TCTGCCTAGC AAGAGTGGCA AGAAAGGAGA TATATGGAAG 
CTCGGTCTCT TGCTACTTTC GCTGTCACAA GGCAACATAG CCAAAGAGTA TCCTGTAAAA 
GTGCCCAGTG ACCTGCCTGC AGATTTCCAA GATTTCCTGC AAAAGTGTGT TTGCTTGGAA 
GACAAGGAAC GCTGGACTAC ACAGCAGTTA CTAAGTCACA GTTTCCTTAA CCCTCCACCT 
GAGAAGAGTC CTGTAACAGA GGAATGCTCA GATGATTATC CGGGTGACTG TACTGAGACT 
GTCATTCCCA GATCTTCAGT ATCTAATTCC ACATTCCATA TTGAGTCACA GAGACAGTTT 
TCTCGCTACT TCAATGAGTT TGAAGAATTA CAGATGCTTG GGAAAGGGGC ATTTGGTGCT 
GTCATTAAGG TTCAGAACAA ACTGGATGGC TGCTATTACG CTGTAAAACG GGTACAAATC 
AATCCTGCAA GTAAACAGTT CCGCAGAATT AAGGGGGAGG TAACATTGTT ATCTCGGCTC 
AACCATGAAA ACATTGTAAG GTACTATAAT GCATGGATTG AGAAACACGA GCATCCTACT 
ATTTCTCCTA AATGTGAAGC ACATGAGGAA AAGAAAGTGA CTGTGAAGCC AGAACAAATA 
GTAAAGAATG ATTTGGGATT GTTGGATGAT ATTGAGGAAA ACGCACCAGC ACCCATATTA 
ACCAGCTCTG TAGAATGGAG TACATCTTGC GAGCGATCAT CCAGTGCGCA CTGTACTAAT 
GGGGATCAAG ATTCAAGTGA TGATGATGAT GATGACGATG AAGAGGGAGT GTTTTCACCT 
TCGTTCCTGC CTGGGACAGA TTCTGACAGT GAGATCATAT TTGAGAATGA AGATGACATT 
AGTCCAGATC AGGATGAAGA ATATGACAAG AACAATGTAG AAGACCGCAA AGCTGGAGCC 
AATGAGATTG TGACTCAGGC AGTGCATTAT TTATATATTC AGATGGAATA TTGTGAGAAA 
AGCACCTTAC GGGACACGAT AGACCAAGGA CTGTATATGG ATGTTGGCCG CCTCTGGAGG 
CTGTTTCGTG AAATTTTGGA TGGATTGACC TATATACATG AGCAGGGAAT GATCCACAGA 
GACCTAAAGC CAGTCAATAT CTTTTTAGAT TCAGAAGATC ATGTCAAAAT AGGAGATTTT 
GGACTAGCTA CAGATCACCC AGCATCTTAT ACTGAATCAG CACAAGGGGA AGAGGATCAG 
GCGCATTCTG ACTTGGTTAA AGTCGACCCT GAAGATAGGG CCTCAACACC TGGTCGCAAT 
ACTCAAGGAA AACTCACTGG CATGGTTGGC ACTGCCTTGT ATGTAAGCCC TGAAGTCCAA 
GGAAACACCA AAGCTTCATA TAACCAGAAA GTGGACCTTT ACAGCCTGGG TATAATATTG 
TTTGAGATGT CCTACCGGCC CATGGACACA AGTTCTGAAC GTATCTCTGT CTTAAGCAGG 
CTTAGACAGA AAAAAGTGAT TACTTGGCTC CTGAACCATG ACCCAGCATC CCGCCCCACC 
GCTATGGAGT TGCTTAAGTG CGATCTCTTA CCCCCACCAC AACTAGAGGA GTCTGAACTC 
CATGAAGTCC TGCACCACAC CTTGGCCAAT ATCGATGGGA AAGCGTACCG CACAATGATG 
AGTCAAATCT TTGCCCAGCG CATTTCACCT GCTATGGATT ATACATATGA CAGTGATATA 
TTGAAGGGTA TTTTCTCAGT CCGTTCTGCC AGACTACATC ATCATGTTTG TGAGGCAGTC 
TGCAGGATAT TTAAAAGACA CGGGGCTGTG AAGTTACATG CCCCACTGCT GATGCCACGA 
GGTAAAGCTG GGTTTGATAA CAGTGAATCT GCATGTTTTA TGGATCACAG TGGCATGCTG 
GTCACCCTTC CTTATAACCT CAGGATATCA TTTGCGCGGT TTGTTGCAAG GAATAATATA 
ACTAGTTTGA AAAGGTATAG CATTGAGAGA GTGTTTAGAC CACGCAAACT GAACCGCTGC 
CATCCAAAGG AACTCACTGA GTGTGCATTT GATATAATCA CTTCTGCTGC AAACTGTTAC 
CTGCCCATTG CAGAAACAAT ATACACCATA TATGAAATTA TCCAGGAATT TCCAGTACTG 
CAGGAAAGGA ATTACAGTAT TTATGTGAAC CACACCTCGT TGTTGAAAGC CATTCTCCTT 
CACTGTGGCG TGCCCGAGGA TAAACTCACT CAGGTGTATA ACATTCTGTA TGATGCAATG 
ATTGAGAAGC TGACAAAAAG AGAAGTGGAA GCCAAATTTT GTAATCTCTC CCTTTCAGAG 
AACAGTTTCC AATTGAGTCG CCTTTACCGG TTTATTGAGC AGAAAGGAGA CCTCCCAGAT 
TTGGAGGCAA TGATGGTCCC ACTCACAAAG CAAAAAACAG GGGTCGGTGT GCTGGCAAAG 
CAAGGTTTGA AAGACTTGGA AGGAGTCATT GGATTAGTGA AGAGGCTGGG CATTAAATTA 
CAGATTGTGG TTAACTTTGG ACTTGTCTAC AAGATGCAGC AGCACAGTGG CATAGTCTTC 
CAGTTTGTGG CTTTTATCAA GCGGAGACAG AGGACTGTGC CTGAAATACT TGCAGCTGGA 
GGCAGATATG ACCATTTGAT TTCCCAGTTT AGGGGACCTC AGACTGGAGC TATGGGATTA 
CCCTGTGCTG TAGGCGTTAG TATAGCAATA GACAAGATAT CTGCTGCGCT TTCCACTGCA 
GAGGAACATA TCAGCAGCTG TGATTTATTA GTCGTAAGCG CTGGGCAGAT GTCTCTGAAT 
AGAGCCATTG GGATAACACA GAAGCTGTGG GCTGCTGGCA TTACTGCAGA GATCATGTAT 
GAGTGGTCTG AGTCTCAGGA TGAACTGCAG GATTATTGCA AGTGTTCGGG GATTTCGTAT 
GCTGCAATTA TTTCTGACAA AGAAGGAAAT CACATTAAGC TGAAAACATT TGAGAAGGAA 
AGACAGAGTG AGAAGAGAAT ATTGGAATCT GATCTAGTGG ATCTCCTGGT GCAGAAACTT 
CGGATCAGAG TCCTTGAAGA AAGAAATAAC AGAGAAACGT CGGACAGTCT CTCAGTGCAA 
AATGTGAAGG GCTCGTTTAA TTCCCAGAGT CAGTTTGAGC CACACAGCGC AATAATTGTG 
CCAAGTGTCA GTGTTATAGC ACCAGAAAAG CTATCTGCCA GTGCCAGGCG GAGATTTGAA 
ACCCAGGTGC TAACAAAGCT TCAGACACTC ATTACAAACT TCCAGCAGAG AAATGGTGAA 
ATTGAAATTT TAGCAGTTGA TCTGCCAAAA GAAACAATAC AGAACTTGTT AGCTTTAGAG 
TGCGCAGATG AACAAGTCTT CAATGCATCA GTGAAAATGC TAATGTCTCG GCTGCCAAAA 
CAGAGATACC TGAAATCAGT GTTTGATGAA ATATACACCA TTAAAATCGA AAAGCGGGTT 
CCTGTGCTGG TACTATACAG TTATAAAGAT GATTATTACA AGATCCTTTT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eif2ak4 ( NM_001256926.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001243855.1 Xenopus tropicalis eukaryotic translation initiation factor 2 alpha kinase 4 (eif2ak4), mRNA.

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CloneID	OXa20310
Clone ID Related Accession (Same CDS sequence)	NM_001256926.1
Accession Version	NM_001256926.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Xenopus tropicalis(tropical clawed frog)
Product	eIF-2-alpha kinase GCN2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAMC03032456.1, AAMC03032457.1 and CX847035.1. On Mar 9, 2012 this sequence version replaced XM_002936897.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00861995, SAMN01758126 [ECO:0000350] ##Evidence-Data-END##

ATGGCAGGCG GCCGGGGAAA TGCACTTCTT CCTATTGAGC TCAGCCCGCA AGGGGGTCTG 
CGCCCTACTC CGCCTGCCTG TGACCCCCCT GAAACTTATT CCCACCGGCA GGAGAATGAG 
CTGGAGGTCC TGGAATCCAT CTATAGTGGG GATTTCGCGG ATCTGCGCAA GAACTCTGCC 
TGGAAGGTAC AGCAGCCTCC AGAAGTCATC ATTGCCTTAC GTCCTCAGGG AAATGAGGTT 
CATGTCAAGG TGGATTTATG GGTAAAATGC CCTCAGAACT ATCCTGATGT TCCTCCTGAA 
ATAAAGCTGA AGAACATCCA GGGGTTGTCC AATGAAAATG TGAACTCTCT GACATCAAGC 
TTGGAAGATT TAGCAAAACA GCTTTGTGGA GAGGTAATGA TATACCAGCT TGCGGATCAT 
GTGCAGTGTT TTCTTACAGA GTATAACCAT CCACCTCCTA AATCCTTTCA TGAGGAGATG 
TTAAAAAACC TTCAGAAGCA GCAGGAGAAG CTTGCTCTTG AAGAAGAGAG ACGAATGCAA 
GAGATAAAGA TGAAAGAAGA GCAGACGAGA CGGGAAATCC TTAATGAAAT CCAGAAGCGA 
GAAGAAGAGA AAAAGGAAGA AAAAAGAAGG AAGGAAGTAG CAAAACAGGA ACGGATTGAA 
AACGCTAATC TAATGTGCCA AGAGAATGTA AATAAGATTA ATAATACAGG CAAGACAACA 
ACAGCTGCCT CGGATGGGTC ACTTCCTGAT CATGGAGGAA CCAACAGAAA TCGGCCACAT 
TCAGCAGGAC GCTCAAAGCG GGACCGACAG TATTCGGTGT GCCTCACTGA TGACTCAACC 
CCATTTGAAG TGTTAACCTT TGATATTGGA TGCAGTGGGC AAATTGTTGT TCACAGAGGA 
AAGTGCCTGG GGAAAGATGA GCTGCTGGGT AAATCTGTTT ACAATGGCTT GGAAACTGCT 
TCAGGACATT TTGTTGTAAT CTATGAATGG GTTCTCCAGT GGCAGAAAAA GATGGGCAAG 
TTTCTTACTA ATCAAGAAAA AGAAAAGATT GAAAAGTGTA AAAAACAGAT TCAGGGAACA 
GAAACCGAGT TCAGTTCTCT TATCAAACTG AGCCATCCAA ACATTGTGCA CTACCTCGCT 
ATGCGAATAA AAGAGAAAGA AGATTCCATT TCTGTCGGGA TCATAGCTGA ACATGTTAAT 
GGTACAAAGC TTTCCAGTTA CCTTGAGCAG GGTGTACCTG TTCCACTGGA TCAACTACGG 
CACTATACTG CCCAGCTGCT ATCGGCACTG GACTATTTGC ATTCTAATTC TGTGGTACAT 
AAAGTCATGT GCGCCTCCAG TGTTTTGGTG ACAGCTGACG GGAAAGTAAA ACTGACAGAC 
TATAGCCTTT CCAAACGCCT TGCTGACATC TGCAAAGAGG ACGTGTTTGA GCAAACAAAA 
GTTCGATTCA GTGAGGATGC TCTGCCTAGC AAGAGTGGCA AGAAAGGAGA TATATGGAAG 
CTCGGTCTCT TGCTACTTTC GCTGTCACAA GGCAACATAG CCAAAGAGTA TCCTGTAAAA 
GTGCCCAGTG ACCTGCCTGC AGATTTCCAA GATTTCCTGC AAAAGTGTGT TTGCTTGGAA 
GACAAGGAAC GCTGGACTAC ACAGCAGTTA CTAAGTCACA GTTTCCTTAA CCCTCCACCT 
GAGAAGAGTC CTGTAACAGA GGAATGCTCA GATGATTATC CGGGTGACTG TACTGAGACT 
GTCATTCCCA GATCTTCAGT ATCTAATTCC ACATTCCATA TTGAGTCACA GAGACAGTTT 
TCTCGCTACT TCAATGAGTT TGAAGAATTA CAGATGCTTG GGAAAGGGGC ATTTGGTGCT 
GTCATTAAGG TTCAGAACAA ACTGGATGGC TGCTATTACG CTGTAAAACG GGTACAAATC 
AATCCTGCAA GTAAACAGTT CCGCAGAATT AAGGGGGAGG TAACATTGTT ATCTCGGCTC 
AACCATGAAA ACATTGTAAG GTACTATAAT GCATGGATTG AGAAACACGA GCATCCTACT 
ATTTCTCCTA AATGTGAAGC ACATGAGGAA AAGAAAGTGA CTGTGAAGCC AGAACAAATA 
GTAAAGAATG ATTTGGGATT GTTGGATGAT ATTGAGGAAA ACGCACCAGC ACCCATATTA 
ACCAGCTCTG TAGAATGGAG TACATCTTGC GAGCGATCAT CCAGTGCGCA CTGTACTAAT 
GGGGATCAAG ATTCAAGTGA TGATGATGAT GATGACGATG AAGAGGGAGT GTTTTCACCT 
TCGTTCCTGC CTGGGACAGA TTCTGACAGT GAGATCATAT TTGAGAATGA AGATGACATT 
AGTCCAGATC AGAACAATGT AGAAGACCGC AAAGCTGGAG CCAATGAGAT TGTGACTCAG 
GCAGTGCATT ATTTATATAT TCAGATGGAA TATTGTGAGA AAAGCACCTT ACGGGACACG 
ATAGACCAAG GACTGTATAT GGATGTTGGC CGCCTCTGGA GGCTGTTTCG TGAAATTTTG 
GATGGATTGA CCTATATACA TGAGCAGGGA ATGATCCACA GAGACCTAAA GCCAGTCAAT 
ATCTTTTTAG ATTCAGAAGA TCATGTCAAA ATAGGAGATT TTGGACTAGC TACAGATCAC 
CCAGCATCTT ATACTGAATC AGCACAAGGG GAAGAGGATC AGGCGCATTC TGACTTGGTT 
AAAGTCGACC CTGAAGATAG GGCCTCAACA CCTGGTCGCA ATACTCAAGG AAAACTCACT 
GGCATGGTTG GCACTGCCTT GTATGTAAGC CCTGAAGTCC AAGGAAACAC CAAAGCTTCA 
TATAACCAGA AAGTGGACCT TTACAGCCTG GGTATAATAT TGTTTGAGAT GTCCTACCGG 
CCCATGGACA CAAGTTCTGA ACGTATCTCT GTCTTAAGCA GGCTTAGACA GGCCTTGATT 
GTGTTCCCAA GTGATTTTGA AAACCCAGAA AATGAAAAAC AGAAAAAAGT GATTACTTGG 
CTCCTGAACC ATGACCCAGC ATCCCGCCCC ACCGCTATGG AGTTGCTTAA GTGCGATCTC 
TTACCCCCAC CACAACTAGA GGAGTCTGAA CTCCATGAAG TCCTGCACCA CACCTTGGCC 
AATATCGATG GGAAAGCGTA CCGCACAATG ATGAGTCAAA TCTTTGCCCA GCGCATTTCA 
CCTGCTATGG ATTATACATA TGACAGTGAT ATATTGAAGG GTATTTTCTC AGTCCGTTCT 
GCCAGACTAC ATCATCATGT TTGTGAGGCA GTCTGCAGGA TATTTAAAAG ACACGGGGCT 
GTGAAGTTAC ATGCCCCACT GCTGATGCCA CGAGGTAAAG CTGGGTTTGA TAACAGTGAA 
TCTGCATGTT TTATGGATCA CAGTGGCATG CTGGTCACCC TTCCTTATAA CCTCAGGATA 
TCATTTGCGC GGTTTGTTGC AAGGAATAAT ATAACTAGTT TGAAAAGGTA TAGCATTGAG 
AGAGTGTTTA GACCACGCAA ACTGAACCGC TGCCATCCAA AGGAACTCAC TGAGTGTGCA 
TTTGATATAA TCACTTCTGC TGCAAACTGT TACCTGCCCA TTGCAGAAAC AATATACACC 
ATATATGAAA TTATCCAGGA ATTTCCAGTA CTGCAGGAAA GGAATTACAG TATTTATGTG 
AACCACACCT CGTTGTTGAA AGCCATTCTC CTTCACTGTG GCGTGCCCGA GGATAAACTC 
ACTCAGGTGT ATAACATTCT GTATGATGCA ATGATTGAGA AGCTGACAAA AAGAGAAGTG 
GAAGCCAAAT TTTGTAATCT CTCCCTTTCA GAGAACAGTT TCCAATTGAG TCGCCTTTAC 
CGGTTTATTG AGCAGAAAGG AGACCTCCCA GATTTGGAGG CAATGATGGT CCCACTCACA 
AAGCAAAAAA CAGGGGTCGG TGTGCTGGCA AAGCAAGGTT TGAAAGACTT GGAAGGAGTC 
ATTGGATTAG TGAAGAGGCT GGGCATTAAA TTACAGATTG TGGTTAACTT TGGACTTGTC 
TACAAGATGC AGCAGCACAG TGGCATAGTC TTCCAGTTTG TGGCTTTTAT CAAGCGGAGA 
CAGAGGACTG TGCCTGAAAT ACTTGCAGCT GGAGGCAGAT ATGACCATTT GATTTCCCAG 
TTTAGGGGAC CTCAGACTGG AGCTATGGGA TTACCCTGTG CTGTAGGCGT TAGTATAGCA 
ATAGACAAGA TATCTGCTGC GCTTTCCACT GCAGAGGAAC ATATCAGCAG CTGTGATTTA 
TTAGTCGTAA GCGCTGGGCA GATGTCTCTG AATAGAGCCA TTGGGATAAC ACAGAAGCTG 
TGGGCTGCTG GCATTACTGC AGAGATCATG TATGAGTGGT CTGAGTCTCA GGATGAACTG 
CAGGATTATT GCAAGTGTTC GGGGATTTCG TATGCTGCAA TTATTTCTGA CAAAGAAGGA 
AATCACATTA AGCTGAAAAC ATTTGAGAAG GAAAGACAGA GTGAGAAGAG AATATTGGAA 
TCTGATCTAG TGGATCTCCT GGTGCAGAAA CTTCGGATCA GAGTCCTTGA AGAAAGAAAT 
AACAGAGAAA CGTCGGACAG TCTCTCAGTG CAAAATGTGA AGGGCTCGTT TAATTCCCAG 
AGTCAGTTTG AGCCACACAG CGCAATAATT GTGCCAAGTG TCAGTGTTAT AGCACCAGAA 
AAGCTATCTG CCAGTGCCAG GCGGAGATTT GAAACCCAGG TGCTAACAAA GCTTCAGACA 
CTCATTACAA ACTTCCAGCA GAGAAATGGT GAAATTGAAA TTTTAGCAGT TGATCTGCCA 
AAAGAAACAA TACAGAACTT GTTAGCTTTA GAGTGCGCAG ATGAACAAGT CTTCAATGCA 
TCAGTGAAAA TGCTAATGTC TCGGCTGCCA AAACAGAGAT ACCTGAAATC AGTGTTTGAT 
GAAATATACA CCATTAAAAT CGAAAAGCGG GTTCCTGTGC TGGTACTATA CAGTTATAAA 
GATGATTATT ACAAGATCCT TTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243855.1
CDS	91..5097
Translation	MAGGRGNALLPIELSPQGGLRPTPPACDPPETYSHRQENELEVLESIYSGDFADLRKNSAWKVQQPPEVIIALRPQGNEVHVKVDLWVKCPQNYPDVPPEIKLKNIQGLSNENVNSLTSSLEDLAKQLCGEVMIYQLADHVQCFLTEYNHPPPKSFHEEMLKNLQKQQEKLALEEERRMQEIKMKEEQTRREILNEIQKREEEKKEEKRRKEVAKQERIENANLMCQENVNKINNTGKTTTAASDGSLPDHGGTNRNRPHSAGRSKRDRQYSVCLTDDSTPFEVLTFDIGCSGQIVVHRGKCLGKDELLGKSVYNGLETASGHFVVIYEWVLQWQKKMGKFLTNQEKEKIEKCKKQIQGTETEFSSLIKLSHPNIVHYLAMRIKEKEDSISVGIIAEHVNGTKLSSYLEQGVPVPLDQLRHYTAQLLSALDYLHSNSVVHKVMCASSVLVTADGKVKLTDYSLSKRLADICKEDVFEQTKVRFSEDALPSKSGKKGDIWKLGLLLLSLSQGNIAKEYPVKVPSDLPADFQDFLQKCVCLEDKERWTTQQLLSHSFLNPPPEKSPVTEECSDDYPGDCTETVIPRSSVSNSTFHIESQRQFSRYFNEFEELQMLGKGAFGAVIKVQNKLDGCYYAVKRVQINPASKQFRRIKGEVTLLSRLNHENIVRYYNAWIEKHEHPTISPKCEAHEEKKVTVKPEQIVKNDLGLLDDIEENAPAPILTSSVEWSTSCERSSSAHCTNGDQDSSDDDDDDDEEGVFSPSFLPGTDSDSEIIFENEDDISPDQNNVEDRKAGANEIVTQAVHYLYIQMEYCEKSTLRDTIDQGLYMDVGRLWRLFREILDGLTYIHEQGMIHRDLKPVNIFLDSEDHVKIGDFGLATDHPASYTESAQGEEDQAHSDLVKVDPEDRASTPGRNTQGKLTGMVGTALYVSPEVQGNTKASYNQKVDLYSLGIILFEMSYRPMDTSSERISVLSRLRQALIVFPSDFENPENEKQKKVITWLLNHDPASRPTAMELLKCDLLPPPQLEESELHEVLHHTLANIDGKAYRTMMSQIFAQRISPAMDYTYDSDILKGIFSVRSARLHHHVCEAVCRIFKRHGAVKLHAPLLMPRGKAGFDNSESACFMDHSGMLVTLPYNLRISFARFVARNNITSLKRYSIERVFRPRKLNRCHPKELTECAFDIITSAANCYLPIAETIYTIYEIIQEFPVLQERNYSIYVNHTSLLKAILLHCGVPEDKLTQVYNILYDAMIEKLTKREVEAKFCNLSLSENSFQLSRLYRFIEQKGDLPDLEAMMVPLTKQKTGVGVLAKQGLKDLEGVIGLVKRLGIKLQIVVNFGLVYKMQQHSGIVFQFVAFIKRRQRTVPEILAAGGRYDHLISQFRGPQTGAMGLPCAVGVSIAIDKISAALSTAEEHISSCDLLVVSAGQMSLNRAIGITQKLWAAGITAEIMYEWSESQDELQDYCKCSGISYAAIISDKEGNHIKLKTFEKERQSEKRILESDLVDLLVQKLRIRVLEERNNRETSDSLSVQNVKGSFNSQSQFEPHSAIIVPSVSVIAPEKLSASARRRFETQVLTKLQTLITNFQQRNGEIEILAVDLPKETIQNLLALECADEQVFNASVKMLMSRLPKQRYLKSVFDEIYTIKIEKRVPVLVLYSYKDDYYKILF

Target ORF information:

RefSeq Version	NM_001256926.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis eukaryotic translation initiation factor 2 alpha kinase 4 (eif2ak4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256926.1

ORF Insert Sequence:

ATGGCAGGCG GCCGGGGAAA TGCACTTCTT CCTATTGAGC TCAGCCCGCA AGGGGGTCTG 
CGCCCTACTC CGCCTGCCTG TGACCCCCCT GAAACTTATT CCCACCGGCA GGAGAATGAG 
CTGGAGGTCC TGGAATCCAT CTATAGTGGG GATTTCGCGG ATCTGCGCAA GAACTCTGCC 
TGGAAGGTAC AGCAGCCTCC AGAAGTCATC ATTGCCTTAC GTCCTCAGGG AAATGAGGTT 
CATGTCAAGG TGGATTTATG GGTAAAATGC CCTCAGAACT ATCCTGATGT TCCTCCTGAA 
ATAAAGCTGA AGAACATCCA GGGGTTGTCC AATGAAAATG TGAACTCTCT GACATCAAGC 
TTGGAAGATT TAGCAAAACA GCTTTGTGGA GAGGTAATGA TATACCAGCT TGCGGATCAT 
GTGCAGTGTT TTCTTACAGA GTATAACCAT CCACCTCCTA AATCCTTTCA TGAGGAGATG 
TTAAAAAACC TTCAGAAGCA GCAGGAGAAG CTTGCTCTTG AAGAAGAGAG ACGAATGCAA 
GAGATAAAGA TGAAAGAAGA GCAGACGAGA CGGGAAATCC TTAATGAAAT CCAGAAGCGA 
GAAGAAGAGA AAAAGGAAGA AAAAAGAAGG AAGGAAGTAG CAAAACAGGA ACGGATTGAA 
AACGCTAATC TAATGTGCCA AGAGAATGTA AATAAGATTA ATAATACAGG CAAGACAACA 
ACAGCTGCCT CGGATGGGTC ACTTCCTGAT CATGGAGGAA CCAACAGAAA TCGGCCACAT 
TCAGCAGGAC GCTCAAAGCG GGACCGACAG TATTCGGTGT GCCTCACTGA TGACTCAACC 
CCATTTGAAG TGTTAACCTT TGATATTGGA TGCAGTGGGC AAATTGTTGT TCACAGAGGA 
AAGTGCCTGG GGAAAGATGA GCTGCTGGGT AAATCTGTTT ACAATGGCTT GGAAACTGCT 
TCAGGACATT TTGTTGTAAT CTATGAATGG GTTCTCCAGT GGCAGAAAAA GATGGGCAAG 
TTTCTTACTA ATCAAGAAAA AGAAAAGATT GAAAAGTGTA AAAAACAGAT TCAGGGAACA 
GAAACCGAGT TCAGTTCTCT TATCAAACTG AGCCATCCAA ACATTGTGCA CTACCTCGCT 
ATGCGAATAA AAGAGAAAGA AGATTCCATT TCTGTCGGGA TCATAGCTGA ACATGTTAAT 
GGTACAAAGC TTTCCAGTTA CCTTGAGCAG GGTGTACCTG TTCCACTGGA TCAACTACGG 
CACTATACTG CCCAGCTGCT ATCGGCACTG GACTATTTGC ATTCTAATTC TGTGGTACAT 
AAAGTCATGT GCGCCTCCAG TGTTTTGGTG ACAGCTGACG GGAAAGTAAA ACTGACAGAC 
TATAGCCTTT CCAAACGCCT TGCTGACATC TGCAAAGAGG ACGTGTTTGA GCAAACAAAA 
GTTCGATTCA GTGAGGATGC TCTGCCTAGC AAGAGTGGCA AGAAAGGAGA TATATGGAAG 
CTCGGTCTCT TGCTACTTTC GCTGTCACAA GGCAACATAG CCAAAGAGTA TCCTGTAAAA 
GTGCCCAGTG ACCTGCCTGC AGATTTCCAA GATTTCCTGC AAAAGTGTGT TTGCTTGGAA 
GACAAGGAAC GCTGGACTAC ACAGCAGTTA CTAAGTCACA GTTTCCTTAA CCCTCCACCT 
GAGAAGAGTC CTGTAACAGA GGAATGCTCA GATGATTATC CGGGTGACTG TACTGAGACT 
GTCATTCCCA GATCTTCAGT ATCTAATTCC ACATTCCATA TTGAGTCACA GAGACAGTTT 
TCTCGCTACT TCAATGAGTT TGAAGAATTA CAGATGCTTG GGAAAGGGGC ATTTGGTGCT 
GTCATTAAGG TTCAGAACAA ACTGGATGGC TGCTATTACG CTGTAAAACG GGTACAAATC 
AATCCTGCAA GTAAACAGTT CCGCAGAATT AAGGGGGAGG TAACATTGTT ATCTCGGCTC 
AACCATGAAA ACATTGTAAG GTACTATAAT GCATGGATTG AGAAACACGA GCATCCTACT 
ATTTCTCCTA AATGTGAAGC ACATGAGGAA AAGAAAGTGA CTGTGAAGCC AGAACAAATA 
GTAAAGAATG ATTTGGGATT GTTGGATGAT ATTGAGGAAA ACGCACCAGC ACCCATATTA 
ACCAGCTCTG TAGAATGGAG TACATCTTGC GAGCGATCAT CCAGTGCGCA CTGTACTAAT 
GGGGATCAAG ATTCAAGTGA TGATGATGAT GATGACGATG AAGAGGGAGT GTTTTCACCT 
TCGTTCCTGC CTGGGACAGA TTCTGACAGT GAGATCATAT TTGAGAATGA AGATGACATT 
AGTCCAGATC AGAACAATGT AGAAGACCGC AAAGCTGGAG CCAATGAGAT TGTGACTCAG 
GCAGTGCATT ATTTATATAT TCAGATGGAA TATTGTGAGA AAAGCACCTT ACGGGACACG 
ATAGACCAAG GACTGTATAT GGATGTTGGC CGCCTCTGGA GGCTGTTTCG TGAAATTTTG 
GATGGATTGA CCTATATACA TGAGCAGGGA ATGATCCACA GAGACCTAAA GCCAGTCAAT 
ATCTTTTTAG ATTCAGAAGA TCATGTCAAA ATAGGAGATT TTGGACTAGC TACAGATCAC 
CCAGCATCTT ATACTGAATC AGCACAAGGG GAAGAGGATC AGGCGCATTC TGACTTGGTT 
AAAGTCGACC CTGAAGATAG GGCCTCAACA CCTGGTCGCA ATACTCAAGG AAAACTCACT 
GGCATGGTTG GCACTGCCTT GTATGTAAGC CCTGAAGTCC AAGGAAACAC CAAAGCTTCA 
TATAACCAGA AAGTGGACCT TTACAGCCTG GGTATAATAT TGTTTGAGAT GTCCTACCGG 
CCCATGGACA CAAGTTCTGA ACGTATCTCT GTCTTAAGCA GGCTTAGACA GGCCTTGATT 
GTGTTCCCAA GTGATTTTGA AAACCCAGAA AATGAAAAAC AGAAAAAAGT GATTACTTGG 
CTCCTGAACC ATGACCCAGC ATCCCGCCCC ACCGCTATGG AGTTGCTTAA GTGCGATCTC 
TTACCCCCAC CACAACTAGA GGAGTCTGAA CTCCATGAAG TCCTGCACCA CACCTTGGCC 
AATATCGATG GGAAAGCGTA CCGCACAATG ATGAGTCAAA TCTTTGCCCA GCGCATTTCA 
CCTGCTATGG ATTATACATA TGACAGTGAT ATATTGAAGG GTATTTTCTC AGTCCGTTCT 
GCCAGACTAC ATCATCATGT TTGTGAGGCA GTCTGCAGGA TATTTAAAAG ACACGGGGCT 
GTGAAGTTAC ATGCCCCACT GCTGATGCCA CGAGGTAAAG CTGGGTTTGA TAACAGTGAA 
TCTGCATGTT TTATGGATCA CAGTGGCATG CTGGTCACCC TTCCTTATAA CCTCAGGATA 
TCATTTGCGC GGTTTGTTGC AAGGAATAAT ATAACTAGTT TGAAAAGGTA TAGCATTGAG 
AGAGTGTTTA GACCACGCAA ACTGAACCGC TGCCATCCAA AGGAACTCAC TGAGTGTGCA 
TTTGATATAA TCACTTCTGC TGCAAACTGT TACCTGCCCA TTGCAGAAAC AATATACACC 
ATATATGAAA TTATCCAGGA ATTTCCAGTA CTGCAGGAAA GGAATTACAG TATTTATGTG 
AACCACACCT CGTTGTTGAA AGCCATTCTC CTTCACTGTG GCGTGCCCGA GGATAAACTC 
ACTCAGGTGT ATAACATTCT GTATGATGCA ATGATTGAGA AGCTGACAAA AAGAGAAGTG 
GAAGCCAAAT TTTGTAATCT CTCCCTTTCA GAGAACAGTT TCCAATTGAG TCGCCTTTAC 
CGGTTTATTG AGCAGAAAGG AGACCTCCCA GATTTGGAGG CAATGATGGT CCCACTCACA 
AAGCAAAAAA CAGGGGTCGG TGTGCTGGCA AAGCAAGGTT TGAAAGACTT GGAAGGAGTC 
ATTGGATTAG TGAAGAGGCT GGGCATTAAA TTACAGATTG TGGTTAACTT TGGACTTGTC 
TACAAGATGC AGCAGCACAG TGGCATAGTC TTCCAGTTTG TGGCTTTTAT CAAGCGGAGA 
CAGAGGACTG TGCCTGAAAT ACTTGCAGCT GGAGGCAGAT ATGACCATTT GATTTCCCAG 
TTTAGGGGAC CTCAGACTGG AGCTATGGGA TTACCCTGTG CTGTAGGCGT TAGTATAGCA 
ATAGACAAGA TATCTGCTGC GCTTTCCACT GCAGAGGAAC ATATCAGCAG CTGTGATTTA 
TTAGTCGTAA GCGCTGGGCA GATGTCTCTG AATAGAGCCA TTGGGATAAC ACAGAAGCTG 
TGGGCTGCTG GCATTACTGC AGAGATCATG TATGAGTGGT CTGAGTCTCA GGATGAACTG 
CAGGATTATT GCAAGTGTTC GGGGATTTCG TATGCTGCAA TTATTTCTGA CAAAGAAGGA 
AATCACATTA AGCTGAAAAC ATTTGAGAAG GAAAGACAGA GTGAGAAGAG AATATTGGAA 
TCTGATCTAG TGGATCTCCT GGTGCAGAAA CTTCGGATCA GAGTCCTTGA AGAAAGAAAT 
AACAGAGAAA CGTCGGACAG TCTCTCAGTG CAAAATGTGA AGGGCTCGTT TAATTCCCAG 
AGTCAGTTTG AGCCACACAG CGCAATAATT GTGCCAAGTG TCAGTGTTAT AGCACCAGAA 
AAGCTATCTG CCAGTGCCAG GCGGAGATTT GAAACCCAGG TGCTAACAAA GCTTCAGACA 
CTCATTACAA ACTTCCAGCA GAGAAATGGT GAAATTGAAA TTTTAGCAGT TGATCTGCCA 
AAAGAAACAA TACAGAACTT GTTAGCTTTA GAGTGCGCAG ATGAACAAGT CTTCAATGCA 
TCAGTGAAAA TGCTAATGTC TCGGCTGCCA AAACAGAGAT ACCTGAAATC AGTGTTTGAT 
GAAATATACA CCATTAAAAT CGAAAAGCGG GTTCCTGTGC TGGTACTATA CAGTTATAAA 
GATGATTATT ACAAGATCCT TTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eif2ak4 ( XM_012968127.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823581.1 Xenopus tropicalis eukaryotic translation initiation factor 2 alpha kinase 4 (eif2ak4), transcript variant X1, mRNA.

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CloneID	OXa32794
Clone ID Related Accession (Same CDS sequence)	XM_012968127.2 , XM_012968127.3
Accession Version	XM_012968127.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5025bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	eIF-2-alpha kinase GCN2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968127.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGCG GCCGGGGAAA TGCACTTCTT CCTATTGAGC TCAGCCCGCA AGGGGGTCTG 
CGCCCTACTC CGCCTGCCTG TGACCCCCCT GAAACTTATT CCCACCGGCA GGAGAATGAG 
CTGGAGGTCC TGGAATCCAT CTATAGTGGG GATTTCGCGG ATCTGCGCAA GAACTCTGCC 
TGGAAGGTAC AGCAGCCTCC AGAAGTCATC ATTGCCTTAC GTCCTCAGGG AAATGAGGTT 
CATGTCAAGG TGGATTTATG GGTAAAATGC CCTCAGAACT ATCCTGATGT TCCTCCTGAA 
ATAAAGCTGA AGAACATCCA GGGGTTGTCC AATGAAAATG TGAACTCTCT GACATCAAGC 
TTGGAAGATT TAGCAAAACA GCTTTGTGGA GAGGTAATGA TATACCAGCT TGCGGATCAT 
GTGCAGTGTT TTCTTACAGA GTATAACCAT CCACCTCCTA AATCCTTTCA TGAGGAGATG 
TTAAAAAACC TTCAGAAGCA GCAGGAGAAG CTTGCTCTTG AAGAAGAGAG ACGAATGCAA 
GAGATAAAGA TGAAAGAAGA GCAGACGAGA CGGGAAATCC TTAATGAAAT CCAGAAGCGA 
GAAGAAGAGA AAAAGGAAGA AAAAAGAAGG AAGGAAGTAG CAAAACAGGA ACGGATTGAA 
AACGCTAATC TAATGTGCCA AGAGAATGTA AATAAGATTA ATAATACAGG CAAGACAACA 
ACAGCTGCCT CGGATGGGTC ACTTCCTGAT CATGGAGGAA CCAACAGAAA TCGGCCACAT 
TCAGCAGGAC GCTCAAAGCG GGACCGACAG TATTCGGTGT GCCTCACTGA TGACTCAACC 
CCATTTGAAG TGTTAACCTT TGATATTGGA TGCAGTGGGC AAATTGTTGT TCACAGAGGA 
AAGTGCCTGG GGAAAGATGA GCTGCTGGGT AAATCTGTTT ACAATGGCTT GGAAACTGCT 
TCAGGACATT TTGTTGTAAT CTATGAATGG GTTCTCCAGT GGCAGAAAAA GATGGGCAAG 
TTTCTTACTA ATCAAGAAAA AGAAAAGATT GAAAAGTGTA AAAAACAGAT TCAGGGAACA 
GAAACCGAGT TCAGTTCTCT TATCAAACTG AGCCATCCAA ACATTGTGCA CTACCTCGCT 
ATGCGAATAA AAGAGAAAGA AGATTCCATT TCTGTCGGGA TCATAGCTGA ACATGTTAAT 
GGTACAAAGC TTTCCAGTTA CCTTGAGCAG GGTGTACCTG TTCCACTGGA TCAACTACGG 
CACTATACTG CCCAGCTGCT ATCGGCACTG GACTATTTGC ATTCTAATTC TGTGGTACAT 
AAAGTCATGT GCGCCTCCAG TGTTTTGGTG ACAGCTGACG GGAAAGTAAA ACTGACAGAC 
TATAGCCTTT CCAAACGCCT TGCTGACATC TGCAAAGAGG ACGTGTTTGA GCAAACAAAA 
GTTCGATTCA GTGAGGATGC TCTGCCTAGC AAGAGTGGCA AGAAAGGAGA TATATGGAAG 
CTCGGTCTCT TGCTACTTTC GCTGTCACAA GGCAACATAG CCAAAGAGTA TCCTGTAAAA 
GTGCCCAGTG ACCTGCCTGC AGATTTCCAA GATTTCCTGC AAAAGTGTGT TTGCTTGGAA 
GACAAGGAAC GCTGGACTAC ACAGCAGTTA CTAAGTCACA GTTTCCTTAA CCCTCCACCT 
GAGAAGAGTC CTGTAACAGA GGAATGCTCA GATGATTATC CGGGTGACTG TACTGAGACT 
GTCATTCCCA GATCTTCAGT ATCTAATTCC ACATTCCATA TTGAGTCACA GAGACAGTTT 
TCTCGCTACT TCAATGAGTT TGAAGAATTA CAGATGCTTG GGAAAGGGGC ATTTGGTGCT 
GTCATTAAGG TTCAGAACAA ACTGGATGGC TGCTATTACG CTGTAAAACG GGTACAAATC 
AATCCTGCAA GTAAACAGTT CCGCAGAATT AAGGGGGAGG TAACATTGTT ATCTCGGCTC 
AACCATGAAA ACATTGTAAG GTACTATAAT GCATGGATTG AGAAACACGA GCATCCTACT 
ATTTCTCCTA AATGTGAAGC ACATGAGGAA AAGAAAGTGA CTGTGAAGCC AGAACAAATA 
GTAAAGAATG ATTTGGGATT GTTGGATGAT ATTGAGGAAA ACGCACCAGC ACCCATATTA 
ACCAGCTCTG TAGAATGGAG TACATCTTGC GAGCGATCAT CCAGTGCGCA CTGTACTAAT 
GGGGATCAAG ATTCAAGTGA TGATGATGAT GATGACGATG AAGAGGGAGT GTTTTCACCT 
TCGTTCCTGC CTGGGACAGA TTCTGACAGT GAGATCATAT TTGAGAATGA AGATGACATT 
AGTCCAGATC AGGATGAAGA ATATGACAAG AACAATGTAG AAGACCGCAA AGCTGGAGCC 
AATGAGATTG TGACTCAGGC AGTGCATTAT TTATATATTC AGATGGAATA TTGTGAGAAA 
AGCACCTTAC GGGACACGAT AGACCAAGGA CTGTATATGG ATGTTGGCCG CCTCTGGAGG 
CTGTTTCGTG AAATTTTGGA TGGATTGACC TATATACATG AGCAGGGAAT GATCCACAGA 
GACCTAAAGC CAGTCAATAT CTTTTTAGAT TCAGAAGATC ATGTCAAAAT AGGAGATTTT 
GGACTAGCTA CAGATCACCC AGCATCTTAT ACTGAATCAG CACAAGGGGA AGAGGATCAG 
GCGCATTCTG ACTTGGTTAA AGTCGACCCT GAAGATAGGG CCTCAACACC TGGTCGCAAT 
ACTCAAGGAA AACTCACTGG CATGGTTGGC ACTGCCTTGT ATGTAAGCCC TGAAGTCCAA 
GGAAACACCA AAGCTTCATA TAACCAGAAA GTGGACCTTT ACAGCCTGGG TATAATATTG 
TTTGAGATGT CCTACCGGCC CATGGACACA AGTTCTGAAC GTATCTCTGT CTTAAGCAGG 
CTTAGACAGG CCTTGATTGT GTTCCCAAGT GATTTTGAAA ACCCAGAAAA TGAAAAACAG 
AAAAAAGTGA TTACTTGGCT CCTGAACCAT GACCCAGCAT CCCGCCCCAC CGCTATGGAG 
TTGCTTAAGT GCGATCTCTT ACCCCCACCA CAACTAGAGG AGTCTGAACT CCATGAAGTC 
CTGCACCACA CCTTGGCCAA TATCGATGGG AAAGCGTACC GCACAATGAT GAGTCAAATC 
TTTGCCCAGC GCATTTCACC TGCTATGGAT TATACATATG ACAGTGATAT ATTGAAGGGT 
ATTTTCTCAG TCCGTTCTGC CAGACTACAT CATCATGTTT GTGAGGCAGT CTGCAGGATA 
TTTAAAAGAC ACGGGGCTGT GAAGTTACAT GCCCCACTGC TGATGCCACG AGGTAAAGCT 
GGGTTTGATA ACAGTGAATC TGCATGTTTT ATGGATCACA GTGGCATGCT GGTCACCCTT 
CCTTATAACC TCAGGATATC ATTTGCGCGG TTTGTTGCAA GGAATAATAT AACTAGTTTG 
AAAAGGTATA GCATTGAGAG AGTGTTTAGA CCACGCAAAC TGAACCGCTG CCATCCAAAG 
GAACTCACTG AGTGTGCATT TGATATAATC ACTTCTGCTG CAAACTGTTA CCTGCCCATT 
GCAGAAACAA TATACACCAT ATATGAAATT ATCCAGGAAT TTCCAGTACT GCAGGAAAGG 
AATTACAGTA TTTATGTGAA CCACACCTCG TTGTTGAAAG CCATTCTCCT TCACTGTGGC 
GTGCCCGAGG ATAAACTCAC TCAGGTGTAT AACATTCTGT ATGATGCAAT GATTGAGAAG 
CTGACAAAAA GAGAAGTGGA AGCCAAATTT TGTAATCTCT CCCTTTCAGA GAACAGTTTC 
CAATTGAGTC GCCTTTACCG GTTTATTGAG CAGAAAGGAG ACCTCCCAGA TTTGGAGGCA 
ATGATGGTCC CACTCACAAA GCAAAAAACA GGGGTCGGTG TGCTGGCAAA GCAAGGTTTG 
AAAGACTTGG AAGGAGTCAT TGGATTAGTG AAGAGGCTGG GCATTAAATT ACAGATTGTG 
GTTAACTTTG GACTTGTCTA CAAGATGCAG CAGCACAGTG GCATAGTCTT CCAGTTTGTG 
GCTTTTATCA AGCGGAGACA GAGGACTGTG CCTGAAATAC TTGCAGCTGG AGGCAGATAT 
GACCATTTGA TTTCCCAGTT TAGGGGACCT CAGACTGGAG CTATGGGATT ACCCTGTGCT 
GTAGGCGTTA GTATAGCAAT AGACAAGATA TCTGCTGCGC TTTCCACTGC AGAGGAACAT 
ATCAGCAGCT GTGATTTATT AGTCGTAAGC GCTGGGCAGA TGTCTCTGAA TAGAGCCATT 
GGGATAACAC AGAAGCTGTG GGCTGCTGGC ATTACTGCAG AGATCATGTA TGAGTGGTCT 
GAGTCTCAGG ATGAACTGCA GGATTATTGC AAGTGTTCGG GGATTTCGTA TGCTGCAATT 
ATTTCTGACA AAGAAGGAAA TCACATTAAG CTGAAAACAT TTGAGAAGGA AAGACAGAGT 
GAGAAGAGAA TATTGGAATC TGATCTAGTG GATCTCCTGG TGCAGAAACT TCGGATCAGA 
GTCCTTGAAG AAAGAAATAA CAGAGAAACG TCGGACAGTC TCTCAGTGCA AAATGTGAAG 
GGCTCGTTTA ATTCCCAGAG TCAGTTTGAG CCACACAGCG CAATAATTGT GCCAAGTGTC 
AGTGTTATAG CACCAGAAAA GCTATCTGCC AGTGCCAGGC GGAGATTTGA AACCCAGGTG 
CTAACAAAGC TTCAGACACT CATTACAAAC TTCCAGCAGA GAAATGGTGA AATTGAAATT 
TTAGCAGTTG ATCTGCCAAA AGAAACAATA CAGAACTTGT TAGCTTTAGA GTGCGCAGAT 
GAACAAGTCT TCAATGCATC AGTGAAAATG CTAATGTCTC GGCTGCCAAA ACAGAGATAC 
CTGAAATCAG TGTTTGATGA AATATACACC ATTAAAATCG AAAAGCGGGT TCCTGTGCTG 
GTACTATACA GTTATAAAGA TGATTATTAC AAGATCCTTT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012823581.1
CDS	648..5672
Translation	MAGGRGNALLPIELSPQGGLRPTPPACDPPETYSHRQENELEVLESIYSGDFADLRKNSAWKVQQPPEVIIALRPQGNEVHVKVDLWVKCPQNYPDVPPEIKLKNIQGLSNENVNSLTSSLEDLAKQLCGEVMIYQLADHVQCFLTEYNHPPPKSFHEEMLKNLQKQQEKLALEEERRMQEIKMKEEQTRREILNEIQKREEEKKEEKRRKEVAKQERIENANLMCQENVNKINNTGKTTTAASDGSLPDHGGTNRNRPHSAGRSKRDRQYSVCLTDDSTPFEVLTFDIGCSGQIVVHRGKCLGKDELLGKSVYNGLETASGHFVVIYEWVLQWQKKMGKFLTNQEKEKIEKCKKQIQGTETEFSSLIKLSHPNIVHYLAMRIKEKEDSISVGIIAEHVNGTKLSSYLEQGVPVPLDQLRHYTAQLLSALDYLHSNSVVHKVMCASSVLVTADGKVKLTDYSLSKRLADICKEDVFEQTKVRFSEDALPSKSGKKGDIWKLGLLLLSLSQGNIAKEYPVKVPSDLPADFQDFLQKCVCLEDKERWTTQQLLSHSFLNPPPEKSPVTEECSDDYPGDCTETVIPRSSVSNSTFHIESQRQFSRYFNEFEELQMLGKGAFGAVIKVQNKLDGCYYAVKRVQINPASKQFRRIKGEVTLLSRLNHENIVRYYNAWIEKHEHPTISPKCEAHEEKKVTVKPEQIVKNDLGLLDDIEENAPAPILTSSVEWSTSCERSSSAHCTNGDQDSSDDDDDDDEEGVFSPSFLPGTDSDSEIIFENEDDISPDQDEEYDKNNVEDRKAGANEIVTQAVHYLYIQMEYCEKSTLRDTIDQGLYMDVGRLWRLFREILDGLTYIHEQGMIHRDLKPVNIFLDSEDHVKIGDFGLATDHPASYTESAQGEEDQAHSDLVKVDPEDRASTPGRNTQGKLTGMVGTALYVSPEVQGNTKASYNQKVDLYSLGIILFEMSYRPMDTSSERISVLSRLRQALIVFPSDFENPENEKQKKVITWLLNHDPASRPTAMELLKCDLLPPPQLEESELHEVLHHTLANIDGKAYRTMMSQIFAQRISPAMDYTYDSDILKGIFSVRSARLHHHVCEAVCRIFKRHGAVKLHAPLLMPRGKAGFDNSESACFMDHSGMLVTLPYNLRISFARFVARNNITSLKRYSIERVFRPRKLNRCHPKELTECAFDIITSAANCYLPIAETIYTIYEIIQEFPVLQERNYSIYVNHTSLLKAILLHCGVPEDKLTQVYNILYDAMIEKLTKREVEAKFCNLSLSENSFQLSRLYRFIEQKGDLPDLEAMMVPLTKQKTGVGVLAKQGLKDLEGVIGLVKRLGIKLQIVVNFGLVYKMQQHSGIVFQFVAFIKRRQRTVPEILAAGGRYDHLISQFRGPQTGAMGLPCAVGVSIAIDKISAALSTAEEHISSCDLLVVSAGQMSLNRAIGITQKLWAAGITAEIMYEWSESQDELQDYCKCSGISYAAIISDKEGNHIKLKTFEKERQSEKRILESDLVDLLVQKLRIRVLEERNNRETSDSLSVQNVKGSFNSQSQFEPHSAIIVPSVSVIAPEKLSASARRRFETQVLTKLQTLITNFQQRNGEIEILAVDLPKETIQNLLALECADEQVFNASVKMLMSRLPKQRYLKSVFDEIYTIKIEKRVPVLVLYSYKDDYYKILF

Target ORF information:

RefSeq Version	XM_012968127.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis eukaryotic translation initiation factor 2 alpha kinase 4 (eif2ak4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968127.2

ORF Insert Sequence:

ATGGCAGGCG GCCGGGGAAA TGCACTTCTT CCTATTGAGC TCAGCCCGCA AGGGGGTCTG 
CGCCCTACTC CGCCTGCCTG TGACCCCCCT GAAACTTATT CCCACCGGCA GGAGAATGAG 
CTGGAGGTCC TGGAATCCAT CTATAGTGGG GATTTCGCGG ATCTGCGCAA GAACTCTGCC 
TGGAAGGTAC AGCAGCCTCC AGAAGTCATC ATTGCCTTAC GTCCTCAGGG AAATGAGGTT 
CATGTCAAGG TGGATTTATG GGTAAAATGC CCTCAGAACT ATCCTGATGT TCCTCCTGAA 
ATAAAGCTGA AGAACATCCA GGGGTTGTCC AATGAAAATG TGAACTCTCT GACATCAAGC 
TTGGAAGATT TAGCAAAACA GCTTTGTGGA GAGGTAATGA TATACCAGCT TGCGGATCAT 
GTGCAGTGTT TTCTTACAGA GTATAACCAT CCACCTCCTA AATCCTTTCA TGAGGAGATG 
TTAAAAAACC TTCAGAAGCA GCAGGAGAAG CTTGCTCTTG AAGAAGAGAG ACGAATGCAA 
GAGATAAAGA TGAAAGAAGA GCAGACGAGA CGGGAAATCC TTAATGAAAT CCAGAAGCGA 
GAAGAAGAGA AAAAGGAAGA AAAAAGAAGG AAGGAAGTAG CAAAACAGGA ACGGATTGAA 
AACGCTAATC TAATGTGCCA AGAGAATGTA AATAAGATTA ATAATACAGG CAAGACAACA 
ACAGCTGCCT CGGATGGGTC ACTTCCTGAT CATGGAGGAA CCAACAGAAA TCGGCCACAT 
TCAGCAGGAC GCTCAAAGCG GGACCGACAG TATTCGGTGT GCCTCACTGA TGACTCAACC 
CCATTTGAAG TGTTAACCTT TGATATTGGA TGCAGTGGGC AAATTGTTGT TCACAGAGGA 
AAGTGCCTGG GGAAAGATGA GCTGCTGGGT AAATCTGTTT ACAATGGCTT GGAAACTGCT 
TCAGGACATT TTGTTGTAAT CTATGAATGG GTTCTCCAGT GGCAGAAAAA GATGGGCAAG 
TTTCTTACTA ATCAAGAAAA AGAAAAGATT GAAAAGTGTA AAAAACAGAT TCAGGGAACA 
GAAACCGAGT TCAGTTCTCT TATCAAACTG AGCCATCCAA ACATTGTGCA CTACCTCGCT 
ATGCGAATAA AAGAGAAAGA AGATTCCATT TCTGTCGGGA TCATAGCTGA ACATGTTAAT 
GGTACAAAGC TTTCCAGTTA CCTTGAGCAG GGTGTACCTG TTCCACTGGA TCAACTACGG 
CACTATACTG CCCAGCTGCT ATCGGCACTG GACTATTTGC ATTCTAATTC TGTGGTACAT 
AAAGTCATGT GCGCCTCCAG TGTTTTGGTG ACAGCTGACG GGAAAGTAAA ACTGACAGAC 
TATAGCCTTT CCAAACGCCT TGCTGACATC TGCAAAGAGG ACGTGTTTGA GCAAACAAAA 
GTTCGATTCA GTGAGGATGC TCTGCCTAGC AAGAGTGGCA AGAAAGGAGA TATATGGAAG 
CTCGGTCTCT TGCTACTTTC GCTGTCACAA GGCAACATAG CCAAAGAGTA TCCTGTAAAA 
GTGCCCAGTG ACCTGCCTGC AGATTTCCAA GATTTCCTGC AAAAGTGTGT TTGCTTGGAA 
GACAAGGAAC GCTGGACTAC ACAGCAGTTA CTAAGTCACA GTTTCCTTAA CCCTCCACCT 
GAGAAGAGTC CTGTAACAGA GGAATGCTCA GATGATTATC CGGGTGACTG TACTGAGACT 
GTCATTCCCA GATCTTCAGT ATCTAATTCC ACATTCCATA TTGAGTCACA GAGACAGTTT 
TCTCGCTACT TCAATGAGTT TGAAGAATTA CAGATGCTTG GGAAAGGGGC ATTTGGTGCT 
GTCATTAAGG TTCAGAACAA ACTGGATGGC TGCTATTACG CTGTAAAACG GGTACAAATC 
AATCCTGCAA GTAAACAGTT CCGCAGAATT AAGGGGGAGG TAACATTGTT ATCTCGGCTC 
AACCATGAAA ACATTGTAAG GTACTATAAT GCATGGATTG AGAAACACGA GCATCCTACT 
ATTTCTCCTA AATGTGAAGC ACATGAGGAA AAGAAAGTGA CTGTGAAGCC AGAACAAATA 
GTAAAGAATG ATTTGGGATT GTTGGATGAT ATTGAGGAAA ACGCACCAGC ACCCATATTA 
ACCAGCTCTG TAGAATGGAG TACATCTTGC GAGCGATCAT CCAGTGCGCA CTGTACTAAT 
GGGGATCAAG ATTCAAGTGA TGATGATGAT GATGACGATG AAGAGGGAGT GTTTTCACCT 
TCGTTCCTGC CTGGGACAGA TTCTGACAGT GAGATCATAT TTGAGAATGA AGATGACATT 
AGTCCAGATC AGGATGAAGA ATATGACAAG AACAATGTAG AAGACCGCAA AGCTGGAGCC 
AATGAGATTG TGACTCAGGC AGTGCATTAT TTATATATTC AGATGGAATA TTGTGAGAAA 
AGCACCTTAC GGGACACGAT AGACCAAGGA CTGTATATGG ATGTTGGCCG CCTCTGGAGG 
CTGTTTCGTG AAATTTTGGA TGGATTGACC TATATACATG AGCAGGGAAT GATCCACAGA 
GACCTAAAGC CAGTCAATAT CTTTTTAGAT TCAGAAGATC ATGTCAAAAT AGGAGATTTT 
GGACTAGCTA CAGATCACCC AGCATCTTAT ACTGAATCAG CACAAGGGGA AGAGGATCAG 
GCGCATTCTG ACTTGGTTAA AGTCGACCCT GAAGATAGGG CCTCAACACC TGGTCGCAAT 
ACTCAAGGAA AACTCACTGG CATGGTTGGC ACTGCCTTGT ATGTAAGCCC TGAAGTCCAA 
GGAAACACCA AAGCTTCATA TAACCAGAAA GTGGACCTTT ACAGCCTGGG TATAATATTG 
TTTGAGATGT CCTACCGGCC CATGGACACA AGTTCTGAAC GTATCTCTGT CTTAAGCAGG 
CTTAGACAGG CCTTGATTGT GTTCCCAAGT GATTTTGAAA ACCCAGAAAA TGAAAAACAG 
AAAAAAGTGA TTACTTGGCT CCTGAACCAT GACCCAGCAT CCCGCCCCAC CGCTATGGAG 
TTGCTTAAGT GCGATCTCTT ACCCCCACCA CAACTAGAGG AGTCTGAACT CCATGAAGTC 
CTGCACCACA CCTTGGCCAA TATCGATGGG AAAGCGTACC GCACAATGAT GAGTCAAATC 
TTTGCCCAGC GCATTTCACC TGCTATGGAT TATACATATG ACAGTGATAT ATTGAAGGGT 
ATTTTCTCAG TCCGTTCTGC CAGACTACAT CATCATGTTT GTGAGGCAGT CTGCAGGATA 
TTTAAAAGAC ACGGGGCTGT GAAGTTACAT GCCCCACTGC TGATGCCACG AGGTAAAGCT 
GGGTTTGATA ACAGTGAATC TGCATGTTTT ATGGATCACA GTGGCATGCT GGTCACCCTT 
CCTTATAACC TCAGGATATC ATTTGCGCGG TTTGTTGCAA GGAATAATAT AACTAGTTTG 
AAAAGGTATA GCATTGAGAG AGTGTTTAGA CCACGCAAAC TGAACCGCTG CCATCCAAAG 
GAACTCACTG AGTGTGCATT TGATATAATC ACTTCTGCTG CAAACTGTTA CCTGCCCATT 
GCAGAAACAA TATACACCAT ATATGAAATT ATCCAGGAAT TTCCAGTACT GCAGGAAAGG 
AATTACAGTA TTTATGTGAA CCACACCTCG TTGTTGAAAG CCATTCTCCT TCACTGTGGC 
GTGCCCGAGG ATAAACTCAC TCAGGTGTAT AACATTCTGT ATGATGCAAT GATTGAGAAG 
CTGACAAAAA GAGAAGTGGA AGCCAAATTT TGTAATCTCT CCCTTTCAGA GAACAGTTTC 
CAATTGAGTC GCCTTTACCG GTTTATTGAG CAGAAAGGAG ACCTCCCAGA TTTGGAGGCA 
ATGATGGTCC CACTCACAAA GCAAAAAACA GGGGTCGGTG TGCTGGCAAA GCAAGGTTTG 
AAAGACTTGG AAGGAGTCAT TGGATTAGTG AAGAGGCTGG GCATTAAATT ACAGATTGTG 
GTTAACTTTG GACTTGTCTA CAAGATGCAG CAGCACAGTG GCATAGTCTT CCAGTTTGTG 
GCTTTTATCA AGCGGAGACA GAGGACTGTG CCTGAAATAC TTGCAGCTGG AGGCAGATAT 
GACCATTTGA TTTCCCAGTT TAGGGGACCT CAGACTGGAG CTATGGGATT ACCCTGTGCT 
GTAGGCGTTA GTATAGCAAT AGACAAGATA TCTGCTGCGC TTTCCACTGC AGAGGAACAT 
ATCAGCAGCT GTGATTTATT AGTCGTAAGC GCTGGGCAGA TGTCTCTGAA TAGAGCCATT 
GGGATAACAC AGAAGCTGTG GGCTGCTGGC ATTACTGCAG AGATCATGTA TGAGTGGTCT 
GAGTCTCAGG ATGAACTGCA GGATTATTGC AAGTGTTCGG GGATTTCGTA TGCTGCAATT 
ATTTCTGACA AAGAAGGAAA TCACATTAAG CTGAAAACAT TTGAGAAGGA AAGACAGAGT 
GAGAAGAGAA TATTGGAATC TGATCTAGTG GATCTCCTGG TGCAGAAACT TCGGATCAGA 
GTCCTTGAAG AAAGAAATAA CAGAGAAACG TCGGACAGTC TCTCAGTGCA AAATGTGAAG 
GGCTCGTTTA ATTCCCAGAG TCAGTTTGAG CCACACAGCG CAATAATTGT GCCAAGTGTC 
AGTGTTATAG CACCAGAAAA GCTATCTGCC AGTGCCAGGC GGAGATTTGA AACCCAGGTG 
CTAACAAAGC TTCAGACACT CATTACAAAC TTCCAGCAGA GAAATGGTGA AATTGAAATT 
TTAGCAGTTG ATCTGCCAAA AGAAACAATA CAGAACTTGT TAGCTTTAGA GTGCGCAGAT 
GAACAAGTCT TCAATGCATC AGTGAAAATG CTAATGTCTC GGCTGCCAAA ACAGAGATAC 
CTGAAATCAG TGTTTGATGA AATATACACC ATTAAAATCG AAAAGCGGGT TCCTGTGCTG 
GTACTATACA GTTATAAAGA TGATTATTAC AAGATCCTTT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eif2ak4 ( XM_012968128.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823582.1 Xenopus tropicalis eukaryotic translation initiation factor 2 alpha kinase 4 (eif2ak4), transcript variant X2, mRNA.

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CloneID	OXa32795
Clone ID Related Accession (Same CDS sequence)	XM_012968128.3 , XM_012968128.2
Accession Version	XM_012968128.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	eIF-2-alpha kinase GCN2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968128.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGCG GCCGGGGAAA TGCACTTCTT CCTATTGAGC TCAGCCCGCA AGGGGGTCTG 
CGCCCTACTC CGCCTGCCTG TGACCCCCCT GAAACTTATT CCCACCGGCA GGAGAATGAG 
CTGGAGGTCC TGGAATCCAT CTATAGTGGG GATTTCGCGG ATCTGCGCAA GAACTCTGCC 
TGGAAGGTAC AGCAGCCTCC AGAAGTCATC ATTGCCTTAC GTCCTCAGGG AAATGAGGTT 
CATGTCAAGG TGGATTTATG GGTAAAATGC CCTCAGAACT ATCCTGATGT TCCTCCTGAA 
ATAAAGCTGA AGAACATCCA GGGGTTGTCC AATGAAAATG TGAACTCTCT GACATCAAGC 
TTGGAAGATT TAGCAAAACA GCTTTGTGGA GAGGTAATGA TATACCAGCT TGCGGATCAT 
GTGCAGTGTT TTCTTACAGA GTATAACCAT CCACCTCCTA AATCCTTTCA TGAGGAGATG 
TTAAAAAACC TTCAGAAGCA GCAGGAGAAG CTTGCTCTTG AAGAAGAGAG ACGAATGCAA 
GAGATAAAGA TGAAAGAAGA GCAGACGAGA CGGGAAATCC TTAATGAAAT CCAGAAGCGA 
GAAGAAGAGA AAAAGGAAGA AAAAAGAAGG AAGGAAGTAG CAAAACAGGA ACGGATTGAA 
AACGCTAATC TAATGTGCCA AGAGAATGTA AATAAGATTA ATAATACAGG CAAGACAACA 
ACAGCTGCCT CGGATGGGTC ACTTCCTGAT CATGGAGGAA CCAACAGAAA TCGGCCACAT 
TCAGCAGGAC GCTCAAAGCG GGACCGACAG TATTCGGTGT GCCTCACTGA TGACTCAACC 
CCATTTGAAG TGTTAACCTT TGATATTGGA TGCAGTGGGC AAATTGTTGT TCACAGAGGA 
AAGTGCCTGG GGAAAGATGA GCTGCTGGGT AAATCTGTTT ACAATGGCTT GGAAACTGCT 
TCAGGACATT TTGTTGTAAT CTATGAATGG GTTCTCCAGT GGCAGAAAAA GATGGGCAAG 
TTTCTTACTA ATCAAGAAAA AGAAAAGATT GAAAAGTGTA AAAAACAGAT TCAGGGAACA 
GAAACCGAGT TCAGTTCTCT TATCAAACTG AGCCATCCAA ACATTGTGCA CTACCTCGCT 
ATGCGAATAA AAGAGAAAGA AGATTCCATT TCTGTCGGGA TCATAGCTGA ACATGTTAAT 
GGTACAAAGC TTTCCAGTTA CCTTGAGCAG GGTGTACCTG TTCCACTGGA TCAACTACGG 
CACTATACTG CCCAGCTGCT ATCGGCACTG GACTATTTGC ATTCTAATTC TGTGGTACAT 
AAAGTCATGT GCGCCTCCAG TGTTTTGGTG ACAGCTGACG GGAAAGTAAA ACTGACAGAC 
TATAGCCTTT CCAAACGCCT TGCTGACATC TGCAAAGAGG ACGTGTTTGA GCAAACAAAA 
GTTCGATTCA GTGAGGATGC TCTGCCTAGC AAGAGTGGCA AGAAAGGAGA TATATGGAAG 
CTCGGTCTCT TGCTACTTTC GCTGTCACAA GGCAACATAG CCAAAGAGTA TCCTGTAAAA 
GTGCCCAGTG ACCTGCCTGC AGATTTCCAA GATTTCCTGC AAAAGTGTGT TTGCTTGGAA 
GACAAGGAAC GCTGGACTAC ACAGCAGTTA CTAAGTCACA GTTTCCTTAA CCCTCCACCT 
GAGAAGAGTC CTGTAACAGA GGAATGCTCA GATGATTATC CGGGTGACTG TACTGAGACT 
GTCATTCCCA GATCTTCAGT ATCTAATTCC ACATTCCATA TTGAGTCACA GAGACAGTTT 
TCTCGCTACT TCAATGAGTT TGAAGAATTA CAGATGCTTG GGAAAGGGGC ATTTGGTGCT 
GTCATTAAGG TTCAGAACAA ACTGGATGGC TGCTATTACG CTGTAAAACG GGTACAAATC 
AATCCTGCAA GTAAACAGTT CCGCAGAATT AAGGGGGAGG TAACATTGTT ATCTCGGCTC 
AACCATGAAA ACATTGTAAG GTACTATAAT GCATGGATTG AGAAACACGA GCATCCTACT 
ATTTCTCCTA AATGTGAAGC ACATGAGGAA AAGAAAGTGA CTGTGAAGCC AGAACAAATA 
GTAAAGAATG ATTTGGGATT GTTGGATGAT ATTGAGGAAA ACGCACCAGC ACCCATATTA 
ACCAGCTCTG TAGAATGGAG TACATCTTGC GAGCGATCAT CCAGTGCGCA CTGTACTAAT 
GGGGATCAAG ATTCAAGTGA TGATGATGAT GATGACGATG AAGAGGGAGT GTTTTCACCT 
TCGTTCCTGC CTGGGACAGA TTCTGACAGT GAGATCATAT TTGAGAATGA AGATGACATT 
AGTCCAGATC AGGATGAAGA ATATGACAAG AACAATGTAG AAGACCGCAA AGCTGGAGCC 
AATGAGATTG TGACTCAGGC AGTGCATTAT TTATATATTC AGATGGAATA TTGTGAGAAA 
AGCACCTTAC GGGACACGAT AGACCAAGGA CTGTATATGG ATGTTGGCCG CCTCTGGAGG 
CTGTTTCGTG AAATTTTGGA TGGATTGACC TATATACATG AGCAGGGAAT GATCCACAGA 
GACCTAAAGC CAGTCAATAT CTTTTTAGAT TCAGAAGATC ATGTCAAAAT AGGAGATTTT 
GGACTAGCTA CAGATCACCC AGCATCTTAT ACTGAATCAG CACAAGGGGA AGAGGATCAG 
GCGCATTCTG ACTTGGTTAA AGTCGACCCT GAAGGAAAAC TCACTGGCAT GGTTGGCACT 
GCCTTGTATG TAAGCCCTGA AGTCCAAGGA AACACCAAAG CTTCATATAA CCAGAAAGTG 
GACCTTTACA GCCTGGGTAT AATATTGTTT GAGATGTCCT ACCGGCCCAT GGACACAAGT 
TCTGAACGTA TCTCTGTCTT AAGCAGGCTT AGACAGGCCT TGATTGTGTT CCCAAGTGAT 
TTTGAAAACC CAGAAAATGA AAAACAGAAA AAAGTGATTA CTTGGCTCCT GAACCATGAC 
CCAGCATCCC GCCCCACCGC TATGGAGTTG CTTAAGTGCG ATCTCTTACC CCCACCACAA 
CTAGAGGAGT CTGAACTCCA TGAAGTCCTG CACCACACCT TGGCCAATAT CGATGGGAAA 
GCGTACCGCA CAATGATGAG TCAAATCTTT GCCCAGCGCA TTTCACCTGC TATGGATTAT 
ACATATGACA GTGATATATT GAAGGGTATT TTCTCAGTCC GTTCTGCCAG ACTACATCAT 
CATGTTTGTG AGGCAGTCTG CAGGATATTT AAAAGACACG GGGCTGTGAA GTTACATGCC 
CCACTGCTGA TGCCACGAGG TAAAGCTGGG TTTGATAACA GTGAATCTGC ATGTTTTATG 
GATCACAGTG GCATGCTGGT CACCCTTCCT TATAACCTCA GGATATCATT TGCGCGGTTT 
GTTGCAAGGA ATAATATAAC TAGTTTGAAA AGGTATAGCA TTGAGAGAGT GTTTAGACCA 
CGCAAACTGA ACCGCTGCCA TCCAAAGGAA CTCACTGAGT GTGCATTTGA TATAATCACT 
TCTGCTGCAA ACTGTTACCT GCCCATTGCA GAAACAATAT ACACCATATA TGAAATTATC 
CAGGAATTTC CAGTACTGCA GGAAAGGAAT TACAGTATTT ATGTGAACCA CACCTCGTTG 
TTGAAAGCCA TTCTCCTTCA CTGTGGCGTG CCCGAGGATA AACTCACTCA GGTGTATAAC 
ATTCTGTATG ATGCAATGAT TGAGAAGCTG ACAAAAAGAG AAGTGGAAGC CAAATTTTGT 
AATCTCTCCC TTTCAGAGAA CAGTTTCCAA TTGAGTCGCC TTTACCGGTT TATTGAGCAG 
AAAGGAGACC TCCCAGATTT GGAGGCAATG ATGGTCCCAC TCACAAAGCA AAAAACAGGG 
GTCGGTGTGC TGGCAAAGCA AGGTTTGAAA GACTTGGAAG GAGTCATTGG ATTAGTGAAG 
AGGCTGGGCA TTAAATTACA GATTGTGGTT AACTTTGGAC TTGTCTACAA GATGCAGCAG 
CACAGTGGCA TAGTCTTCCA GTTTGTGGCT TTTATCAAGC GGAGACAGAG GACTGTGCCT 
GAAATACTTG CAGCTGGAGG CAGATATGAC CATTTGATTT CCCAGTTTAG GGGACCTCAG 
ACTGGAGCTA TGGGATTACC CTGTGCTGTA GGCGTTAGTA TAGCAATAGA CAAGATATCT 
GCTGCGCTTT CCACTGCAGA GGAACATATC AGCAGCTGTG ATTTATTAGT CGTAAGCGCT 
GGGCAGATGT CTCTGAATAG AGCCATTGGG ATAACACAGA AGCTGTGGGC TGCTGGCATT 
ACTGCAGAGA TCATGTATGA GTGGTCTGAG TCTCAGGATG AACTGCAGGA TTATTGCAAG 
TGTTCGGGGA TTTCGTATGC TGCAATTATT TCTGACAAAG AAGGAAATCA CATTAAGCTG 
AAAACATTTG AGAAGGAAAG ACAGAGTGAG AAGAGAATAT TGGAATCTGA TCTAGTGGAT 
CTCCTGGTGC AGAAACTTCG GATCAGAGTC CTTGAAGAAA GAAATAACAG AGAAACGTCG 
GACAGTCTCT CAGTGCAAAA TGTGAAGGGC TCGTTTAATT CCCAGAGTCA GTTTGAGCCA 
CACAGCGCAA TAATTGTGCC AAGTGTCAGT GTTATAGCAC CAGAAAAGCT ATCTGCCAGT 
GCCAGGCGGA GATTTGAAAC CCAGGTGCTA ACAAAGCTTC AGACACTCAT TACAAACTTC 
CAGCAGAGAA ATGGTGAAAT TGAAATTTTA GCAGTTGATC TGCCAAAAGA AACAATACAG 
AACTTGTTAG CTTTAGAGTG CGCAGATGAA CAAGTCTTCA ATGCATCAGT GAAAATGCTA 
ATGTCTCGGC TGCCAAAACA GAGATACCTG AAATCAGTGT TTGATGAAAT ATACACCATT 
AAAATCGAAA AGCGGGTTCC TGTGCTGGTA CTATACAGTT ATAAAGATGA TTATTACAAG 
ATCCTTTTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012823582.1
CDS	647..5638
Translation	MAGGRGNALLPIELSPQGGLRPTPPACDPPETYSHRQENELEVLESIYSGDFADLRKNSAWKVQQPPEVIIALRPQGNEVHVKVDLWVKCPQNYPDVPPEIKLKNIQGLSNENVNSLTSSLEDLAKQLCGEVMIYQLADHVQCFLTEYNHPPPKSFHEEMLKNLQKQQEKLALEEERRMQEIKMKEEQTRREILNEIQKREEEKKEEKRRKEVAKQERIENANLMCQENVNKINNTGKTTTAASDGSLPDHGGTNRNRPHSAGRSKRDRQYSVCLTDDSTPFEVLTFDIGCSGQIVVHRGKCLGKDELLGKSVYNGLETASGHFVVIYEWVLQWQKKMGKFLTNQEKEKIEKCKKQIQGTETEFSSLIKLSHPNIVHYLAMRIKEKEDSISVGIIAEHVNGTKLSSYLEQGVPVPLDQLRHYTAQLLSALDYLHSNSVVHKVMCASSVLVTADGKVKLTDYSLSKRLADICKEDVFEQTKVRFSEDALPSKSGKKGDIWKLGLLLLSLSQGNIAKEYPVKVPSDLPADFQDFLQKCVCLEDKERWTTQQLLSHSFLNPPPEKSPVTEECSDDYPGDCTETVIPRSSVSNSTFHIESQRQFSRYFNEFEELQMLGKGAFGAVIKVQNKLDGCYYAVKRVQINPASKQFRRIKGEVTLLSRLNHENIVRYYNAWIEKHEHPTISPKCEAHEEKKVTVKPEQIVKNDLGLLDDIEENAPAPILTSSVEWSTSCERSSSAHCTNGDQDSSDDDDDDDEEGVFSPSFLPGTDSDSEIIFENEDDISPDQDEEYDKNNVEDRKAGANEIVTQAVHYLYIQMEYCEKSTLRDTIDQGLYMDVGRLWRLFREILDGLTYIHEQGMIHRDLKPVNIFLDSEDHVKIGDFGLATDHPASYTESAQGEEDQAHSDLVKVDPEGKLTGMVGTALYVSPEVQGNTKASYNQKVDLYSLGIILFEMSYRPMDTSSERISVLSRLRQALIVFPSDFENPENEKQKKVITWLLNHDPASRPTAMELLKCDLLPPPQLEESELHEVLHHTLANIDGKAYRTMMSQIFAQRISPAMDYTYDSDILKGIFSVRSARLHHHVCEAVCRIFKRHGAVKLHAPLLMPRGKAGFDNSESACFMDHSGMLVTLPYNLRISFARFVARNNITSLKRYSIERVFRPRKLNRCHPKELTECAFDIITSAANCYLPIAETIYTIYEIIQEFPVLQERNYSIYVNHTSLLKAILLHCGVPEDKLTQVYNILYDAMIEKLTKREVEAKFCNLSLSENSFQLSRLYRFIEQKGDLPDLEAMMVPLTKQKTGVGVLAKQGLKDLEGVIGLVKRLGIKLQIVVNFGLVYKMQQHSGIVFQFVAFIKRRQRTVPEILAAGGRYDHLISQFRGPQTGAMGLPCAVGVSIAIDKISAALSTAEEHISSCDLLVVSAGQMSLNRAIGITQKLWAAGITAEIMYEWSESQDELQDYCKCSGISYAAIISDKEGNHIKLKTFEKERQSEKRILESDLVDLLVQKLRIRVLEERNNRETSDSLSVQNVKGSFNSQSQFEPHSAIIVPSVSVIAPEKLSASARRRFETQVLTKLQTLITNFQQRNGEIEILAVDLPKETIQNLLALECADEQVFNASVKMLMSRLPKQRYLKSVFDEIYTIKIEKRVPVLVLYSYKDDYYKILF

Target ORF information:

RefSeq Version	XM_012968128.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis eukaryotic translation initiation factor 2 alpha kinase 4 (eif2ak4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968128.2

ORF Insert Sequence:

ATGGCAGGCG GCCGGGGAAA TGCACTTCTT CCTATTGAGC TCAGCCCGCA AGGGGGTCTG 
CGCCCTACTC CGCCTGCCTG TGACCCCCCT GAAACTTATT CCCACCGGCA GGAGAATGAG 
CTGGAGGTCC TGGAATCCAT CTATAGTGGG GATTTCGCGG ATCTGCGCAA GAACTCTGCC 
TGGAAGGTAC AGCAGCCTCC AGAAGTCATC ATTGCCTTAC GTCCTCAGGG AAATGAGGTT 
CATGTCAAGG TGGATTTATG GGTAAAATGC CCTCAGAACT ATCCTGATGT TCCTCCTGAA 
ATAAAGCTGA AGAACATCCA GGGGTTGTCC AATGAAAATG TGAACTCTCT GACATCAAGC 
TTGGAAGATT TAGCAAAACA GCTTTGTGGA GAGGTAATGA TATACCAGCT TGCGGATCAT 
GTGCAGTGTT TTCTTACAGA GTATAACCAT CCACCTCCTA AATCCTTTCA TGAGGAGATG 
TTAAAAAACC TTCAGAAGCA GCAGGAGAAG CTTGCTCTTG AAGAAGAGAG ACGAATGCAA 
GAGATAAAGA TGAAAGAAGA GCAGACGAGA CGGGAAATCC TTAATGAAAT CCAGAAGCGA 
GAAGAAGAGA AAAAGGAAGA AAAAAGAAGG AAGGAAGTAG CAAAACAGGA ACGGATTGAA 
AACGCTAATC TAATGTGCCA AGAGAATGTA AATAAGATTA ATAATACAGG CAAGACAACA 
ACAGCTGCCT CGGATGGGTC ACTTCCTGAT CATGGAGGAA CCAACAGAAA TCGGCCACAT 
TCAGCAGGAC GCTCAAAGCG GGACCGACAG TATTCGGTGT GCCTCACTGA TGACTCAACC 
CCATTTGAAG TGTTAACCTT TGATATTGGA TGCAGTGGGC AAATTGTTGT TCACAGAGGA 
AAGTGCCTGG GGAAAGATGA GCTGCTGGGT AAATCTGTTT ACAATGGCTT GGAAACTGCT 
TCAGGACATT TTGTTGTAAT CTATGAATGG GTTCTCCAGT GGCAGAAAAA GATGGGCAAG 
TTTCTTACTA ATCAAGAAAA AGAAAAGATT GAAAAGTGTA AAAAACAGAT TCAGGGAACA 
GAAACCGAGT TCAGTTCTCT TATCAAACTG AGCCATCCAA ACATTGTGCA CTACCTCGCT 
ATGCGAATAA AAGAGAAAGA AGATTCCATT TCTGTCGGGA TCATAGCTGA ACATGTTAAT 
GGTACAAAGC TTTCCAGTTA CCTTGAGCAG GGTGTACCTG TTCCACTGGA TCAACTACGG 
CACTATACTG CCCAGCTGCT ATCGGCACTG GACTATTTGC ATTCTAATTC TGTGGTACAT 
AAAGTCATGT GCGCCTCCAG TGTTTTGGTG ACAGCTGACG GGAAAGTAAA ACTGACAGAC 
TATAGCCTTT CCAAACGCCT TGCTGACATC TGCAAAGAGG ACGTGTTTGA GCAAACAAAA 
GTTCGATTCA GTGAGGATGC TCTGCCTAGC AAGAGTGGCA AGAAAGGAGA TATATGGAAG 
CTCGGTCTCT TGCTACTTTC GCTGTCACAA GGCAACATAG CCAAAGAGTA TCCTGTAAAA 
GTGCCCAGTG ACCTGCCTGC AGATTTCCAA GATTTCCTGC AAAAGTGTGT TTGCTTGGAA 
GACAAGGAAC GCTGGACTAC ACAGCAGTTA CTAAGTCACA GTTTCCTTAA CCCTCCACCT 
GAGAAGAGTC CTGTAACAGA GGAATGCTCA GATGATTATC CGGGTGACTG TACTGAGACT 
GTCATTCCCA GATCTTCAGT ATCTAATTCC ACATTCCATA TTGAGTCACA GAGACAGTTT 
TCTCGCTACT TCAATGAGTT TGAAGAATTA CAGATGCTTG GGAAAGGGGC ATTTGGTGCT 
GTCATTAAGG TTCAGAACAA ACTGGATGGC TGCTATTACG CTGTAAAACG GGTACAAATC 
AATCCTGCAA GTAAACAGTT CCGCAGAATT AAGGGGGAGG TAACATTGTT ATCTCGGCTC 
AACCATGAAA ACATTGTAAG GTACTATAAT GCATGGATTG AGAAACACGA GCATCCTACT 
ATTTCTCCTA AATGTGAAGC ACATGAGGAA AAGAAAGTGA CTGTGAAGCC AGAACAAATA 
GTAAAGAATG ATTTGGGATT GTTGGATGAT ATTGAGGAAA ACGCACCAGC ACCCATATTA 
ACCAGCTCTG TAGAATGGAG TACATCTTGC GAGCGATCAT CCAGTGCGCA CTGTACTAAT 
GGGGATCAAG ATTCAAGTGA TGATGATGAT GATGACGATG AAGAGGGAGT GTTTTCACCT 
TCGTTCCTGC CTGGGACAGA TTCTGACAGT GAGATCATAT TTGAGAATGA AGATGACATT 
AGTCCAGATC AGGATGAAGA ATATGACAAG AACAATGTAG AAGACCGCAA AGCTGGAGCC 
AATGAGATTG TGACTCAGGC AGTGCATTAT TTATATATTC AGATGGAATA TTGTGAGAAA 
AGCACCTTAC GGGACACGAT AGACCAAGGA CTGTATATGG ATGTTGGCCG CCTCTGGAGG 
CTGTTTCGTG AAATTTTGGA TGGATTGACC TATATACATG AGCAGGGAAT GATCCACAGA 
GACCTAAAGC CAGTCAATAT CTTTTTAGAT TCAGAAGATC ATGTCAAAAT AGGAGATTTT 
GGACTAGCTA CAGATCACCC AGCATCTTAT ACTGAATCAG CACAAGGGGA AGAGGATCAG 
GCGCATTCTG ACTTGGTTAA AGTCGACCCT GAAGGAAAAC TCACTGGCAT GGTTGGCACT 
GCCTTGTATG TAAGCCCTGA AGTCCAAGGA AACACCAAAG CTTCATATAA CCAGAAAGTG 
GACCTTTACA GCCTGGGTAT AATATTGTTT GAGATGTCCT ACCGGCCCAT GGACACAAGT 
TCTGAACGTA TCTCTGTCTT AAGCAGGCTT AGACAGGCCT TGATTGTGTT CCCAAGTGAT 
TTTGAAAACC CAGAAAATGA AAAACAGAAA AAAGTGATTA CTTGGCTCCT GAACCATGAC 
CCAGCATCCC GCCCCACCGC TATGGAGTTG CTTAAGTGCG ATCTCTTACC CCCACCACAA 
CTAGAGGAGT CTGAACTCCA TGAAGTCCTG CACCACACCT TGGCCAATAT CGATGGGAAA 
GCGTACCGCA CAATGATGAG TCAAATCTTT GCCCAGCGCA TTTCACCTGC TATGGATTAT 
ACATATGACA GTGATATATT GAAGGGTATT TTCTCAGTCC GTTCTGCCAG ACTACATCAT 
CATGTTTGTG AGGCAGTCTG CAGGATATTT AAAAGACACG GGGCTGTGAA GTTACATGCC 
CCACTGCTGA TGCCACGAGG TAAAGCTGGG TTTGATAACA GTGAATCTGC ATGTTTTATG 
GATCACAGTG GCATGCTGGT CACCCTTCCT TATAACCTCA GGATATCATT TGCGCGGTTT 
GTTGCAAGGA ATAATATAAC TAGTTTGAAA AGGTATAGCA TTGAGAGAGT GTTTAGACCA 
CGCAAACTGA ACCGCTGCCA TCCAAAGGAA CTCACTGAGT GTGCATTTGA TATAATCACT 
TCTGCTGCAA ACTGTTACCT GCCCATTGCA GAAACAATAT ACACCATATA TGAAATTATC 
CAGGAATTTC CAGTACTGCA GGAAAGGAAT TACAGTATTT ATGTGAACCA CACCTCGTTG 
TTGAAAGCCA TTCTCCTTCA CTGTGGCGTG CCCGAGGATA AACTCACTCA GGTGTATAAC 
ATTCTGTATG ATGCAATGAT TGAGAAGCTG ACAAAAAGAG AAGTGGAAGC CAAATTTTGT 
AATCTCTCCC TTTCAGAGAA CAGTTTCCAA TTGAGTCGCC TTTACCGGTT TATTGAGCAG 
AAAGGAGACC TCCCAGATTT GGAGGCAATG ATGGTCCCAC TCACAAAGCA AAAAACAGGG 
GTCGGTGTGC TGGCAAAGCA AGGTTTGAAA GACTTGGAAG GAGTCATTGG ATTAGTGAAG 
AGGCTGGGCA TTAAATTACA GATTGTGGTT AACTTTGGAC TTGTCTACAA GATGCAGCAG 
CACAGTGGCA TAGTCTTCCA GTTTGTGGCT TTTATCAAGC GGAGACAGAG GACTGTGCCT 
GAAATACTTG CAGCTGGAGG CAGATATGAC CATTTGATTT CCCAGTTTAG GGGACCTCAG 
ACTGGAGCTA TGGGATTACC CTGTGCTGTA GGCGTTAGTA TAGCAATAGA CAAGATATCT 
GCTGCGCTTT CCACTGCAGA GGAACATATC AGCAGCTGTG ATTTATTAGT CGTAAGCGCT 
GGGCAGATGT CTCTGAATAG AGCCATTGGG ATAACACAGA AGCTGTGGGC TGCTGGCATT 
ACTGCAGAGA TCATGTATGA GTGGTCTGAG TCTCAGGATG AACTGCAGGA TTATTGCAAG 
TGTTCGGGGA TTTCGTATGC TGCAATTATT TCTGACAAAG AAGGAAATCA CATTAAGCTG 
AAAACATTTG AGAAGGAAAG ACAGAGTGAG AAGAGAATAT TGGAATCTGA TCTAGTGGAT 
CTCCTGGTGC AGAAACTTCG GATCAGAGTC CTTGAAGAAA GAAATAACAG AGAAACGTCG 
GACAGTCTCT CAGTGCAAAA TGTGAAGGGC TCGTTTAATT CCCAGAGTCA GTTTGAGCCA 
CACAGCGCAA TAATTGTGCC AAGTGTCAGT GTTATAGCAC CAGAAAAGCT ATCTGCCAGT 
GCCAGGCGGA GATTTGAAAC CCAGGTGCTA ACAAAGCTTC AGACACTCAT TACAAACTTC 
CAGCAGAGAA ATGGTGAAAT TGAAATTTTA GCAGTTGATC TGCCAAAAGA AACAATACAG 
AACTTGTTAG CTTTAGAGTG CGCAGATGAA CAAGTCTTCA ATGCATCAGT GAAAATGCTA 
ATGTCTCGGC TGCCAAAACA GAGATACCTG AAATCAGTGT TTGATGAAAT ATACACCATT 
AAAATCGAAA AGCGGGTTCC TGTGCTGGTA CTATACAGTT ATAAAGATGA TTATTACAAG 
ATCCTTTTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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eif2ak4 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

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