PFAS cDNA ORF clone, Xenopus tropicalis(Tropical clawed frog)

The following PFAS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PFAS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa65930 XM_012970190.3
Latest version!
Xenopus tropicalis phosphoribosylformylglycinamidine synthase (pfas), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa65931 XM_012970192.3
Latest version!
Xenopus tropicalis phosphoribosylformylglycinamidine synthase (pfas), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa02847 NM_001142220.2
Latest version!
Xenopus (Silurana) tropicalis phosphoribosylformylglycinamidine synthase (pfas), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa32769 NM_001142220.3
Latest version!
Xenopus tropicalis phosphoribosylformylglycinamidine synthase (pfas), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa32769 XM_012970191.2
Latest version!
Xenopus tropicalis phosphoribosylformylglycinamidine synthase (pfas), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa32769 XM_012970190.2
Latest version!
Xenopus tropicalis phosphoribosylformylglycinamidine synthase (pfas), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa32770 XM_012970192.2
Latest version!
Xenopus tropicalis phosphoribosylformylglycinamidine synthase (pfas), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa65930
    Clone ID Related Accession (Same CDS sequence) XM_012970190.3
    Accession Version XM_012970190.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3975bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product phosphoribosylformylglycinamidine synthase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970190.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGTGGTCC TGCACTTCTA TCGCTCCTTG CAGAATTCAT CAGAGACCCT GCGGCTGCCC 
    GGTGCGGAGA ATATACAGAG CCTGCAGAGG GAGCTGTGCT ACAATGTCAA TTGGACAGGT
    CCCGCGGCCC CATCCAGCCA GGAGACTGAC GCCCTTCGAT GGCTGTTTAG TTGCCCCTTT
    GACCCTCAGA GTATCTCTGA CACCTCATCC CTTCACTCGG AGCCTTCTGA CCTGCTGGTA
    GAGATCGGCC CAAGACTGAA TTTCTCCACC GCGACTTCCA CCAATGCTGT GTCCATCTGC
    CGCTCTGTTG GCCTGACCAA CGTGGACAGG ATCGAGTGCT CCAAGAGGTA TCTCATCAAG
    TTCCAGACTA GGCCCCAGGG CAGTGAGGAG CAAGAGCTGC TAGCCTCTCT GTACGACCGT
    ATGACGGAAT GCGTTTACCC GGAGCCGGTG AGGAGTTTCG AGGTGTCCGT TAGACCGGAG
    AAAGTGTACG AGGTGGATAT TTTGGGGCAA GGCAGGGCAG CACTGGAAAA GGCCAACACT
    GAGCTAGGTT TGGCCTTCGA CTCCTGGGAT CTTGATTACT ATAGCAGCCT TTTCCAGCGC
    GTGGGTCGGA ACCCCAGCAG CGTGGAGTGC TTTGATCTTG CACAGTCCAA CAGCGAGCAC
    AGCCGCCACT GGTTCTTTAA GGGCAAATTA AAGGTCGACG GGCAGGAGAA GGAGATGTCT
    CTGTTCGACA TGATCATGAA GACTCAGGAC ACCAGTAATC CCAACAACGT CATCAAGTTC
    TGTGACAACA GCAGTGCGAT CCAGGGAAGA GAAGTGGCAT CTCTAATCCC AACAGACCCT
    TCATGTGCTG GGCCCTATCA TCTCTGTCGC TCCACACGGC ACCTGATTTT CACTGCAGAG
    ACCCACAACT TCCCTACTGG CGTGGCCCCA TTCAGCGGCG CTACGACAGG AACAGGAGGG
    CGCATTCGGG ACGTGCAGAG CACCGGCAGG GGAGCACATG TCATTGCTGG GACGGCTGGG
    TACTGCTTTG GGAACCTGCA CATCCCAGGA TACTCTCTTC CCTGGGAGGA CCCCGCATAT
    CAGTACCCAG TGCAGTTTGC CAGGCCTCTG GAGGTGGCTA TTGAAGCCAG TAACGGAGCC
    TCTGATTATG GAAACAAGTT TGGGGAGCCT GTTTTGGCCG GATTTGCCCG TTCCTTTGGA
    GTGCGGTTGC CTTCGGGAGA AAGGAGGGAG TGGGTGAAAC CCATTATGTT CAGTGGTGGG
    ATCGGATCCA TGGAGGATGT TCACCGTACT AAGGAGGCCC CAGAGCCTGG CATGCACGTG
    GTTAAAGTGG GAGGTCCTGT CTATAGGATT GGGGTTGGCG GTGGAGCTGC CTCTTCTATT
    CAGGTCCAGG GGGATAACGC CAGCGAGCTG GATTTTGGGG CAGTGCAACG TGGAGATGCA
    GAGATGGAGC AGAAGATGAA TCGGGCCGTA CGGGCCTGCG TGGAGCGAGG AGGCAAGAAC
    CCCATCTGCA GCATTCACGA TCAGGGAGCC GGGGGCAACG GGAACGTCCT GAAGGAGCTC
    AGTGAGCCCC AAGGAGCAGT CATTTACACT AAAAGCTTCC AGCTGGGGGA CCCCACCCTG
    AGTGTTCTGG AGATATGGGG AGCAGAATAT CAGGAGTCCA ACGCTTTGCT CCTGCGCCCC
    GACGATGCCG AATTCCTGCG CTCTGTGTGC CGGCGGGAGA GGTCTCCTGT GGATTTTGTG
    GGCAAGATCA CCGGGGATGG CAGGATTGTG TTGATAAATG GCAGTGACAC AGATCCCGCC
    CCTGACTCCA CTGACCGCAA TGCTGTTCCC GTTGACCTCC AGCTGGAATG GGTCTTAGGA
    AAAATGCCTC GCAAGGAGTT TGTTCTAAAC CGCGTGGCAC CAAACTTGCA GCCGCTGGTG
    CTCCCTAGTG GCCTGACAGT CGGGCAGGCG CTTGATCGCG TGCTGAGGTT GCCGTCCGTA
    GCGAGCAAAC GCTACCTGAC CAACAAGGTG GACCGCTCCG TCACAGGCCT GGTGGCCCAG
    CAGCAGTGCG TGGGGCCTCT TCACACTCCA TTGGCCGACG TGGCTGTGGT GTCTCTGTCT
    TATTCGGACA TTGTAGGAGG AGCTACAGCA ATAGGAGAAC AGCCAATCAA AAGCCTACTG
    AATCCTGCAG CTGGGGCAAG ACTGGCGGTG GCCGAGGCAC TAACCAATCT CCTCTTTGCT
    CAAGTGACGG ACCTAAAGGA TGTGAAATGC AGCGGGAACT GGATGTGGGC CGCCAAGCTG
    CCCGGAGAAG GCGCCTTGCT TTACGACGCC TGTGCAGCTA TGTGTGATGT CATGGCGCAG
    CTCGGCGTTG CCATCGATGG AGGAAAAGAT TCTCTCAGCA TGGCCGCCAG AGTGGGCACA
    GAAACAGTTA AAGCTCCAGG TTCGCTAGTG ATCTCGGTGT ATGCAGTCTG CCCCGACATA
    ACTACCACCG TGACCCCCGA TCTTAAGAAC CCAGGGCAGA AAGGTGTTTT ACTGTATTTG
    CCCCTCTGCC CTGGTCAGCA CCGGCTTGGG GGCAGCGCCC TGGCTCAGTG CTACTCACAG
    CTGGGAGAGA CCCCCCCAGA CCTCGATGAC CCTCAGACTT TAATCTCCTG CTTCAACGTC
    ACCCAACAGC TGCTGAGAGA CCGCGTCCTT AGCGCCGGCC ATGACGTCAG TGATGGAGGC
    CTAGTCACCT GTATCCTGGA AATGGCTTTT GCTGGAAACT GTGGCCTGGA TATTGAGATT
    TCCTCCTCCT GCACCAATGT GCTGGAACTG CTGTTTGCCG AGGAACCGGG CCTGGTTCTA
    GAGGTTGCAG AACAAAGTGT AGAACTGGTC ATGGAACGTT ACAGAGCAGC AGGAGTGGAA
    TGCGTGAGGA TCGGATGCAC CAAGGAACGT GGCCCAGCGT CTATGGTCCG AATCCGTGCC
    AATGGGCAGG AGGTGGTCAA CGAGAAGTTG GGTTCCCTCA GAGCCGTATG GGAGGAGACC
    AGTTTCCAAC TTGAACGCCT TCAGGCCAAT CCAAGCTGTG TATCGCAGGA GGAGGCTGGG
    CTGAGAGTGA GGGAGGGGCC AAGTTATCAC CTGACTTTTA ACCCGTCTGA AATACCTTTG
    GTTCCTCCAT CAGTCGGTGG CAACCAACCG CGTGTTGCTG TTGTGCGTGA AGAGGGTAGC
    AACGGAGACC GGGAAATGGC GGCATCTCTC CTAATGGCTG GATTTCAGGT GTGGGATGTC
    ACCATGGAAG ATCTTCTTGC TGGAGGCACC ACCCTGGACT CTTTCAGGGG ACTTGTATTT
    GTTGGGGGAT TTAGCTACGC TGATGTGTTA GGGTCTGCCA AAGGTTGGGC CGCCTCGGTG
    AAGTTCAATG CCAGCGTGCG CGAGCAGTTC GAGAACTTCC GCCGTAGGGC AGATACTTTC
    AGCCTTGGGG TTTGCAATGG GTGCCAGCTG ATGGCTCTTC TGGGCTGGGT AGGACCCGAT
    AACCCAACAG ACACAGTAGG TGATCTCCCC ACCCAAGGGG TTCTGCTCAG CCACAATCTC
    TCCGGCCGGT TTGAATCTCG ATTTGTCACC CTGAAGATCC AACAGAGTCC TTCCATTTTG
    CTACGAGGGA TGGCCGGGTC CACGCTCGGA GTGTGGGTGG CCCATGGCGA AGGATACATG
    AGGTTCCGAT CTCCGAAAGT GCAGGACTAT GTGACGTCCA ATCACTTGGC GCCGCTCTGT
    TACGTGGATG ATGGAGGCAA GCCGACGGAA GAATACCCAA TGAATCCAAA TGGCTCTCCG
    TTGGGAATTG CTGGTCTTTG CTCTGCAGAT GGGCGGCACT TGGCCATGAT GCCCCACCCA
    GAGCGCTGTG TCATGAAGTG GCAGTGGCCT TGGATGCCGG AAAGCTGGCG CCACTCATTG
    GATGTCTCGC CGTGGATGCG TCTCTTCCAG AATGGGTACC GCTGGTGCCA GGAGAACCCA
    AGCAGGGATA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012825644.2
    CDS101..4075
    Translation

    Target ORF information:

    RefSeq Version XM_012970190.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phosphoribosylformylglycinamidine synthase (pfas), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970190.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGTGGTCC TGCACTTCTA TCGCTCCTTG CAGAATTCAT CAGAGACCCT GCGGCTGCCC 
    GGTGCGGAGA ATATACAGAG CCTGCAGAGG GAGCTGTGCT ACAATGTCAA TTGGACAGGT
    CCCGCGGCCC CATCCAGCCA GGAGACTGAC GCCCTTCGAT GGCTGTTTAG TTGCCCCTTT
    GACCCTCAGA GTATCTCTGA CACCTCATCC CTTCACTCGG AGCCTTCTGA CCTGCTGGTA
    GAGATCGGCC CAAGACTGAA TTTCTCCACC GCGACTTCCA CCAATGCTGT GTCCATCTGC
    CGCTCTGTTG GCCTGACCAA CGTGGACAGG ATCGAGTGCT CCAAGAGGTA TCTCATCAAG
    TTCCAGACTA GGCCCCAGGG CAGTGAGGAG CAAGAGCTGC TAGCCTCTCT GTACGACCGT
    ATGACGGAAT GCGTTTACCC GGAGCCGGTG AGGAGTTTCG AGGTGTCCGT TAGACCGGAG
    AAAGTGTACG AGGTGGATAT TTTGGGGCAA GGCAGGGCAG CACTGGAAAA GGCCAACACT
    GAGCTAGGTT TGGCCTTCGA CTCCTGGGAT CTTGATTACT ATAGCAGCCT TTTCCAGCGC
    GTGGGTCGGA ACCCCAGCAG CGTGGAGTGC TTTGATCTTG CACAGTCCAA CAGCGAGCAC
    AGCCGCCACT GGTTCTTTAA GGGCAAATTA AAGGTCGACG GGCAGGAGAA GGAGATGTCT
    CTGTTCGACA TGATCATGAA GACTCAGGAC ACCAGTAATC CCAACAACGT CATCAAGTTC
    TGTGACAACA GCAGTGCGAT CCAGGGAAGA GAAGTGGCAT CTCTAATCCC AACAGACCCT
    TCATGTGCTG GGCCCTATCA TCTCTGTCGC TCCACACGGC ACCTGATTTT CACTGCAGAG
    ACCCACAACT TCCCTACTGG CGTGGCCCCA TTCAGCGGCG CTACGACAGG AACAGGAGGG
    CGCATTCGGG ACGTGCAGAG CACCGGCAGG GGAGCACATG TCATTGCTGG GACGGCTGGG
    TACTGCTTTG GGAACCTGCA CATCCCAGGA TACTCTCTTC CCTGGGAGGA CCCCGCATAT
    CAGTACCCAG TGCAGTTTGC CAGGCCTCTG GAGGTGGCTA TTGAAGCCAG TAACGGAGCC
    TCTGATTATG GAAACAAGTT TGGGGAGCCT GTTTTGGCCG GATTTGCCCG TTCCTTTGGA
    GTGCGGTTGC CTTCGGGAGA AAGGAGGGAG TGGGTGAAAC CCATTATGTT CAGTGGTGGG
    ATCGGATCCA TGGAGGATGT TCACCGTACT AAGGAGGCCC CAGAGCCTGG CATGCACGTG
    GTTAAAGTGG GAGGTCCTGT CTATAGGATT GGGGTTGGCG GTGGAGCTGC CTCTTCTATT
    CAGGTCCAGG GGGATAACGC CAGCGAGCTG GATTTTGGGG CAGTGCAACG TGGAGATGCA
    GAGATGGAGC AGAAGATGAA TCGGGCCGTA CGGGCCTGCG TGGAGCGAGG AGGCAAGAAC
    CCCATCTGCA GCATTCACGA TCAGGGAGCC GGGGGCAACG GGAACGTCCT GAAGGAGCTC
    AGTGAGCCCC AAGGAGCAGT CATTTACACT AAAAGCTTCC AGCTGGGGGA CCCCACCCTG
    AGTGTTCTGG AGATATGGGG AGCAGAATAT CAGGAGTCCA ACGCTTTGCT CCTGCGCCCC
    GACGATGCCG AATTCCTGCG CTCTGTGTGC CGGCGGGAGA GGTCTCCTGT GGATTTTGTG
    GGCAAGATCA CCGGGGATGG CAGGATTGTG TTGATAAATG GCAGTGACAC AGATCCCGCC
    CCTGACTCCA CTGACCGCAA TGCTGTTCCC GTTGACCTCC AGCTGGAATG GGTCTTAGGA
    AAAATGCCTC GCAAGGAGTT TGTTCTAAAC CGCGTGGCAC CAAACTTGCA GCCGCTGGTG
    CTCCCTAGTG GCCTGACAGT CGGGCAGGCG CTTGATCGCG TGCTGAGGTT GCCGTCCGTA
    GCGAGCAAAC GCTACCTGAC CAACAAGGTG GACCGCTCCG TCACAGGCCT GGTGGCCCAG
    CAGCAGTGCG TGGGGCCTCT TCACACTCCA TTGGCCGACG TGGCTGTGGT GTCTCTGTCT
    TATTCGGACA TTGTAGGAGG AGCTACAGCA ATAGGAGAAC AGCCAATCAA AAGCCTACTG
    AATCCTGCAG CTGGGGCAAG ACTGGCGGTG GCCGAGGCAC TAACCAATCT CCTCTTTGCT
    CAAGTGACGG ACCTAAAGGA TGTGAAATGC AGCGGGAACT GGATGTGGGC CGCCAAGCTG
    CCCGGAGAAG GCGCCTTGCT TTACGACGCC TGTGCAGCTA TGTGTGATGT CATGGCGCAG
    CTCGGCGTTG CCATCGATGG AGGAAAAGAT TCTCTCAGCA TGGCCGCCAG AGTGGGCACA
    GAAACAGTTA AAGCTCCAGG TTCGCTAGTG ATCTCGGTGT ATGCAGTCTG CCCCGACATA
    ACTACCACCG TGACCCCCGA TCTTAAGAAC CCAGGGCAGA AAGGTGTTTT ACTGTATTTG
    CCCCTCTGCC CTGGTCAGCA CCGGCTTGGG GGCAGCGCCC TGGCTCAGTG CTACTCACAG
    CTGGGAGAGA CCCCCCCAGA CCTCGATGAC CCTCAGACTT TAATCTCCTG CTTCAACGTC
    ACCCAACAGC TGCTGAGAGA CCGCGTCCTT AGCGCCGGCC ATGACGTCAG TGATGGAGGC
    CTAGTCACCT GTATCCTGGA AATGGCTTTT GCTGGAAACT GTGGCCTGGA TATTGAGATT
    TCCTCCTCCT GCACCAATGT GCTGGAACTG CTGTTTGCCG AGGAACCGGG CCTGGTTCTA
    GAGGTTGCAG AACAAAGTGT AGAACTGGTC ATGGAACGTT ACAGAGCAGC AGGAGTGGAA
    TGCGTGAGGA TCGGATGCAC CAAGGAACGT GGCCCAGCGT CTATGGTCCG AATCCGTGCC
    AATGGGCAGG AGGTGGTCAA CGAGAAGTTG GGTTCCCTCA GAGCCGTATG GGAGGAGACC
    AGTTTCCAAC TTGAACGCCT TCAGGCCAAT CCAAGCTGTG TATCGCAGGA GGAGGCTGGG
    CTGAGAGTGA GGGAGGGGCC AAGTTATCAC CTGACTTTTA ACCCGTCTGA AATACCTTTG
    GTTCCTCCAT CAGTCGGTGG CAACCAACCG CGTGTTGCTG TTGTGCGTGA AGAGGGTAGC
    AACGGAGACC GGGAAATGGC GGCATCTCTC CTAATGGCTG GATTTCAGGT GTGGGATGTC
    ACCATGGAAG ATCTTCTTGC TGGAGGCACC ACCCTGGACT CTTTCAGGGG ACTTGTATTT
    GTTGGGGGAT TTAGCTACGC TGATGTGTTA GGGTCTGCCA AAGGTTGGGC CGCCTCGGTG
    AAGTTCAATG CCAGCGTGCG CGAGCAGTTC GAGAACTTCC GCCGTAGGGC AGATACTTTC
    AGCCTTGGGG TTTGCAATGG GTGCCAGCTG ATGGCTCTTC TGGGCTGGGT AGGACCCGAT
    AACCCAACAG ACACAGTAGG TGATCTCCCC ACCCAAGGGG TTCTGCTCAG CCACAATCTC
    TCCGGCCGGT TTGAATCTCG ATTTGTCACC CTGAAGATCC AACAGAGTCC TTCCATTTTG
    CTACGAGGGA TGGCCGGGTC CACGCTCGGA GTGTGGGTGG CCCATGGCGA AGGATACATG
    AGGTTCCGAT CTCCGAAAGT GCAGGACTAT GTGACGTCCA ATCACTTGGC GCCGCTCTGT
    TACGTGGATG ATGGAGGCAA GCCGACGGAA GAATACCCAA TGAATCCAAA TGGCTCTCCG
    TTGGGAATTG CTGGTCTTTG CTCTGCAGAT GGGCGGCACT TGGCCATGAT GCCCCACCCA
    GAGCGCTGTG TCATGAAGTG GCAGTGGCCT TGGATGCCGG AAAGCTGGCG CCACTCATTG
    GATGTCTCGC CGTGGATGCG TCTCTTCCAG AATGGGTACC GCTGGTGCCA GGAGAACCCA
    AGCAGGGATA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa65931
    Clone ID Related Accession (Same CDS sequence) XM_012970192.3
    Accession Version XM_012970192.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3972bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product phosphoribosylformylglycinamidine synthase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970192.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGTGGTCC TGCACTTCTA TCGCTCCTTG CAGAATTCAT CAGAGACCCT GCGGCTGCCC 
    GGTGCGGAGA ATATACAGAG CCTGCAGAGG GAGCTGTGCT ACAATGTCAA TTGGACAGGT
    CCCGCGGCCC CATCCAGCCA GGAGACTGAC GCCCTTCGAT GGCTGTTTAG TTGCCCCTTT
    GACCCTCAGA GTATCTCTGA CACCTCATCC CTTCACTCGG AGCCTTCTGA CCTGCTGGTA
    GAGATCGGCC CAAGACTGAA TTTCTCCACC GCGACTTCCA CCAATGCTGT GTCCATCTGC
    CGCTCTGTTG GCCTGACCAA CGTGGACAGG ATCGAGTGCT CCAAGAGGTA TCTCATCAAG
    TTCCAGACTA GGCCCCAGGG CAGTGAGGAG CAAGAGCTGC TAGCCTCTCT GTACGACCGT
    ATGACGGAAT GCGTTTACCC GGAGCCGGTG AGGAGTTTCG AGGTGTCCGT TAGACCGGAG
    AAAGTGTACG AGGTGGATAT TTTGGGGCAA GGCAGGGCAG CACTGGAAAA GGCCAACACT
    GAGCTAGGTT TGGCCTTCGA CTCCTGGGAT CTTGATTACT ATAGCAGCCT TTTCCAGCGC
    GTGGGTCGGA ACCCCAGCAG CGTGGAGTGC TTTGATCTTG CACAGTCCAA CAGCGAGCAC
    AGCCGCCACT GGTTCTTTAA GGGCAAATTA AAGGTCGACG GGCAGGAGAA GGAGATGTCT
    CTGTTCGACA TGATCATGAA GACTCAGGAC ACCAGTAATC CCAACAACGT CATCAAGTTC
    TGTGACAACA GCAGTGCGAT CCAGGGAAGA GAAGTGGCAT CTCTAATCCC AACAGACCCT
    TCATGTGCTG GGCCCTATCA TCTCTGTCGC TCCACACGGC ACCTGATTTT CACTGCAGAG
    ACCCACAACT TCCCTACTGG CGTGGCCCCA TTCAGCGGCG CTACGACAGG AACAGGAGGG
    CGCATTCGGG ACGTGCAGAG CACCGGCAGG GGAGCACATG TCATTGCTGG GACGGCTGGG
    TACTGCTTTG GGAACCTGCA CATCCCAGGA TACTCTCTTC CCTGGGAGGA CCCCGCATAT
    CAGTACCCAG TGCAGTTTGC CAGGCCTCTG GAGGTGGCTA TTGAAGCCAG TAACGGAGCC
    TCTGATTATG GAAACAAGTT TGGGGAGCCT GTTTTGGCCG GATTTGCCCG TTCCTTTGGA
    GTGCGGTTGC CTTCGGGAGA AAGGAGGGAG TGGGTGAAAC CCATTATGTT CAGTGGTGGG
    ATCGGATCCA TGGAGGATGT TCACCGTACT AAGGAGGCCC CAGAGCCTGG CATGCACGTG
    GTTAAAGTGG GAGGTCCTGT CTATAGGATT GGGGTTGGCG GTGGAGCTGC CTCTTCTATT
    CAGGTCCAGG GGGATAACGC CAGCGAGCTG GATTTTGGGG CAGTGCAACG TGGAGATGCA
    GAGATGGAGC AGAAGATGAA TCGGGCCGTA CGGGCCTGCG TGGAGCGAGG AGGCAAGAAC
    CCCATCTGCA GCATTCACGA TCAGGGAGCC GGGGGCAACG GGAACGTCCT GAAGGAGCTC
    AGTGAGCCCC AAGGAGCAGT CATTTACACT AAAAGCTTCC AGCTGGGGGA CCCCACCCTG
    AGTGTTCTGG AGATATGGGG AGCAGAATAT CAGGAGTCCA ACGCTTTGCT CCTGCGCCCC
    GACGATGCCG AATTCCTGCG CTCTGTGTGC CGGCGGGAGA GGTCTCCTGT GGATTTTGTG
    GGCAAGATCA CCGGGGATGG CAGGATTGTG TTGATAAATG GCAGTGACAC AGATCCCGCC
    CCTGACTCCA CTGACCGCAA TGCTGTTCCC GTTGACCTCC AGCTGGAATG GGTCTTAGGA
    AAAATGCCTC GCAAGGAGTT TGTTCTAAAC CGCGTGGCAC CAAACTTGCA GCCGCTGGTG
    CTCCCTAGTG GCCTGACAGT CGGGCAGGCG CTTGATCGCG TGCTGAGGTT GCCGTCCGTA
    GCGAGCAAAC GCTACCTGAC CAACAAGGTG GACCGCTCCG TCACAGGCCT GGTGGCCCAG
    CAGCAGTGCG TGGGGCCTCT TCACACTCCA TTGGCCGACG TGGCTGTGGT GTCTCTGTCT
    TATTCGGACA TTGTAGGAGG AGCTACAGCA ATAGGAGAAC AGCCAATCAA AAGCCTACTG
    AATCCTGCAG CTGGGGCAAG ACTGGCGGTG GCCGAGGCAC TAACCAATCT CCTCTTTGCT
    CAAGTGACGG ACCTAAAGGA TGTGAAATGC AGCGGGAACT GGATGTGGGC CGCCAAGCTG
    CCCGGAGAAG GCGCCTTGCT TTACGACGCC TGTGCAGCTA TGTGTGATGT CATGGCGCAG
    CTCGGCGTTG CCATCGATGG AGGAAAAGAT TCTCTCAGCA TGGCCGCCAG AGTGGGCACA
    GAAACAGTTA AAGCTCCAGG TTCGCTAGTG ATCTCGGTGT ATGCAGTCTG CCCCGACATA
    ACTACCACCG TGACCCCCGA TCTTAAGAAC CCAGGGCAGA AAGGTGTTTT ACTGTATTTG
    CCCCTCTGCC CTGGTCAGCA CCGGCTTGGG GGCAGCGCCC TGGCTCAGTG CTACTCACAG
    CTGGGAGAGA CCCCCCCAGA CCTCGATGAC CCTCAGACTT TAATCTCCTG CTTCAACGTC
    ACCCAACAGC TGCTGAGAGA CCGCGTCCTT AGCGCCGGCC ATGACGTCAG TGATGGAGGC
    CTAGTCACCT GTATCCTGGA AATGGCTTTT GCTGGAAACT GTGGCCTGGA TATTGAGATT
    TCCTCCTCCT GCACCAATGT GCTGGAACTG CTGTTTGCCG AGGAACCGGG CCTGGTTCTA
    GAGGTTGCAG AACAAAGTGT AGAACTGGTC ATGGAACGTT ACAGAGCAGC AGGAGTGGAA
    TGCGTGAGGA TCGGATGCAC CAAGGAACGT GGCCCAGCGT CTATGGTCCG AATCCGTGCC
    AATGGGCAGG AGGTGGTCAA CGAGAAGTTG GGTTCCCTCA GAGCCGTATG GGAGGAGACC
    AGTTTCCAAC TTGAACGCCT TCAGGCCAAT CCAAGCTGTG TATCGCAGGA GGAGGCTGGG
    CTGAGAGTGA GGGAGGGGCC AAGTTATCAC CTGACTTTTA ACCCGTCTGA AATACCTTTG
    GTTCCTCCAT CAGTCGGTGG CAACCAACCG CGTGTTGCTG TTGTGCGTGA AGAGGGTAGC
    AACGGAGACC GGGAAATGGC GGCATCTCTC CTAATGGCTG GATTTCAGGT GTGGGATGTC
    ACCATGGAAG ATCTTCTTGC TGGAGGCACC ACCCTGGACT CTTTCAGGGG ACTTGTATTT
    GTTGGGGGAT TTAGCTACGC TGATGTGTTA GGGTCTGCCA AAGGTTGGGC CGCCTCGGTG
    AAGTTCAATG CCAGCGTGCG CGAGCAGTTC GAGAACTTCC GCCGTAGGGC AGATACTTTC
    AGCCTTGGGG TTTGCAATGG GTGCCAGCTG ATGGCTCTTC TGGGCTGGGT AGGACCCGAT
    AACCCAACAG ACACAGGTGA TCTCCCCACC CAAGGGGTTC TGCTCAGCCA CAATCTCTCC
    GGCCGGTTTG AATCTCGATT TGTCACCCTG AAGATCCAAC AGAGTCCTTC CATTTTGCTA
    CGAGGGATGG CCGGGTCCAC GCTCGGAGTG TGGGTGGCCC ATGGCGAAGG ATACATGAGG
    TTCCGATCTC CGAAAGTGCA GGACTATGTG ACGTCCAATC ACTTGGCGCC GCTCTGTTAC
    GTGGATGATG GAGGCAAGCC GACGGAAGAA TACCCAATGA ATCCAAATGG CTCTCCGTTG
    GGAATTGCTG GTCTTTGCTC TGCAGATGGG CGGCACTTGG CCATGATGCC CCACCCAGAG
    CGCTGTGTCA TGAAGTGGCA GTGGCCTTGG ATGCCGGAAA GCTGGCGCCA CTCATTGGAT
    GTCTCGCCGT GGATGCGTCT CTTCCAGAAT GGGTACCGCT GGTGCCAGGA GAACCCAAGC
    AGGGATAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012825646.2
    CDS112..4083
    Translation

    Target ORF information:

    RefSeq Version XM_012970192.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phosphoribosylformylglycinamidine synthase (pfas), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970192.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGTGGTCC TGCACTTCTA TCGCTCCTTG CAGAATTCAT CAGAGACCCT GCGGCTGCCC 
    GGTGCGGAGA ATATACAGAG CCTGCAGAGG GAGCTGTGCT ACAATGTCAA TTGGACAGGT
    CCCGCGGCCC CATCCAGCCA GGAGACTGAC GCCCTTCGAT GGCTGTTTAG TTGCCCCTTT
    GACCCTCAGA GTATCTCTGA CACCTCATCC CTTCACTCGG AGCCTTCTGA CCTGCTGGTA
    GAGATCGGCC CAAGACTGAA TTTCTCCACC GCGACTTCCA CCAATGCTGT GTCCATCTGC
    CGCTCTGTTG GCCTGACCAA CGTGGACAGG ATCGAGTGCT CCAAGAGGTA TCTCATCAAG
    TTCCAGACTA GGCCCCAGGG CAGTGAGGAG CAAGAGCTGC TAGCCTCTCT GTACGACCGT
    ATGACGGAAT GCGTTTACCC GGAGCCGGTG AGGAGTTTCG AGGTGTCCGT TAGACCGGAG
    AAAGTGTACG AGGTGGATAT TTTGGGGCAA GGCAGGGCAG CACTGGAAAA GGCCAACACT
    GAGCTAGGTT TGGCCTTCGA CTCCTGGGAT CTTGATTACT ATAGCAGCCT TTTCCAGCGC
    GTGGGTCGGA ACCCCAGCAG CGTGGAGTGC TTTGATCTTG CACAGTCCAA CAGCGAGCAC
    AGCCGCCACT GGTTCTTTAA GGGCAAATTA AAGGTCGACG GGCAGGAGAA GGAGATGTCT
    CTGTTCGACA TGATCATGAA GACTCAGGAC ACCAGTAATC CCAACAACGT CATCAAGTTC
    TGTGACAACA GCAGTGCGAT CCAGGGAAGA GAAGTGGCAT CTCTAATCCC AACAGACCCT
    TCATGTGCTG GGCCCTATCA TCTCTGTCGC TCCACACGGC ACCTGATTTT CACTGCAGAG
    ACCCACAACT TCCCTACTGG CGTGGCCCCA TTCAGCGGCG CTACGACAGG AACAGGAGGG
    CGCATTCGGG ACGTGCAGAG CACCGGCAGG GGAGCACATG TCATTGCTGG GACGGCTGGG
    TACTGCTTTG GGAACCTGCA CATCCCAGGA TACTCTCTTC CCTGGGAGGA CCCCGCATAT
    CAGTACCCAG TGCAGTTTGC CAGGCCTCTG GAGGTGGCTA TTGAAGCCAG TAACGGAGCC
    TCTGATTATG GAAACAAGTT TGGGGAGCCT GTTTTGGCCG GATTTGCCCG TTCCTTTGGA
    GTGCGGTTGC CTTCGGGAGA AAGGAGGGAG TGGGTGAAAC CCATTATGTT CAGTGGTGGG
    ATCGGATCCA TGGAGGATGT TCACCGTACT AAGGAGGCCC CAGAGCCTGG CATGCACGTG
    GTTAAAGTGG GAGGTCCTGT CTATAGGATT GGGGTTGGCG GTGGAGCTGC CTCTTCTATT
    CAGGTCCAGG GGGATAACGC CAGCGAGCTG GATTTTGGGG CAGTGCAACG TGGAGATGCA
    GAGATGGAGC AGAAGATGAA TCGGGCCGTA CGGGCCTGCG TGGAGCGAGG AGGCAAGAAC
    CCCATCTGCA GCATTCACGA TCAGGGAGCC GGGGGCAACG GGAACGTCCT GAAGGAGCTC
    AGTGAGCCCC AAGGAGCAGT CATTTACACT AAAAGCTTCC AGCTGGGGGA CCCCACCCTG
    AGTGTTCTGG AGATATGGGG AGCAGAATAT CAGGAGTCCA ACGCTTTGCT CCTGCGCCCC
    GACGATGCCG AATTCCTGCG CTCTGTGTGC CGGCGGGAGA GGTCTCCTGT GGATTTTGTG
    GGCAAGATCA CCGGGGATGG CAGGATTGTG TTGATAAATG GCAGTGACAC AGATCCCGCC
    CCTGACTCCA CTGACCGCAA TGCTGTTCCC GTTGACCTCC AGCTGGAATG GGTCTTAGGA
    AAAATGCCTC GCAAGGAGTT TGTTCTAAAC CGCGTGGCAC CAAACTTGCA GCCGCTGGTG
    CTCCCTAGTG GCCTGACAGT CGGGCAGGCG CTTGATCGCG TGCTGAGGTT GCCGTCCGTA
    GCGAGCAAAC GCTACCTGAC CAACAAGGTG GACCGCTCCG TCACAGGCCT GGTGGCCCAG
    CAGCAGTGCG TGGGGCCTCT TCACACTCCA TTGGCCGACG TGGCTGTGGT GTCTCTGTCT
    TATTCGGACA TTGTAGGAGG AGCTACAGCA ATAGGAGAAC AGCCAATCAA AAGCCTACTG
    AATCCTGCAG CTGGGGCAAG ACTGGCGGTG GCCGAGGCAC TAACCAATCT CCTCTTTGCT
    CAAGTGACGG ACCTAAAGGA TGTGAAATGC AGCGGGAACT GGATGTGGGC CGCCAAGCTG
    CCCGGAGAAG GCGCCTTGCT TTACGACGCC TGTGCAGCTA TGTGTGATGT CATGGCGCAG
    CTCGGCGTTG CCATCGATGG AGGAAAAGAT TCTCTCAGCA TGGCCGCCAG AGTGGGCACA
    GAAACAGTTA AAGCTCCAGG TTCGCTAGTG ATCTCGGTGT ATGCAGTCTG CCCCGACATA
    ACTACCACCG TGACCCCCGA TCTTAAGAAC CCAGGGCAGA AAGGTGTTTT ACTGTATTTG
    CCCCTCTGCC CTGGTCAGCA CCGGCTTGGG GGCAGCGCCC TGGCTCAGTG CTACTCACAG
    CTGGGAGAGA CCCCCCCAGA CCTCGATGAC CCTCAGACTT TAATCTCCTG CTTCAACGTC
    ACCCAACAGC TGCTGAGAGA CCGCGTCCTT AGCGCCGGCC ATGACGTCAG TGATGGAGGC
    CTAGTCACCT GTATCCTGGA AATGGCTTTT GCTGGAAACT GTGGCCTGGA TATTGAGATT
    TCCTCCTCCT GCACCAATGT GCTGGAACTG CTGTTTGCCG AGGAACCGGG CCTGGTTCTA
    GAGGTTGCAG AACAAAGTGT AGAACTGGTC ATGGAACGTT ACAGAGCAGC AGGAGTGGAA
    TGCGTGAGGA TCGGATGCAC CAAGGAACGT GGCCCAGCGT CTATGGTCCG AATCCGTGCC
    AATGGGCAGG AGGTGGTCAA CGAGAAGTTG GGTTCCCTCA GAGCCGTATG GGAGGAGACC
    AGTTTCCAAC TTGAACGCCT TCAGGCCAAT CCAAGCTGTG TATCGCAGGA GGAGGCTGGG
    CTGAGAGTGA GGGAGGGGCC AAGTTATCAC CTGACTTTTA ACCCGTCTGA AATACCTTTG
    GTTCCTCCAT CAGTCGGTGG CAACCAACCG CGTGTTGCTG TTGTGCGTGA AGAGGGTAGC
    AACGGAGACC GGGAAATGGC GGCATCTCTC CTAATGGCTG GATTTCAGGT GTGGGATGTC
    ACCATGGAAG ATCTTCTTGC TGGAGGCACC ACCCTGGACT CTTTCAGGGG ACTTGTATTT
    GTTGGGGGAT TTAGCTACGC TGATGTGTTA GGGTCTGCCA AAGGTTGGGC CGCCTCGGTG
    AAGTTCAATG CCAGCGTGCG CGAGCAGTTC GAGAACTTCC GCCGTAGGGC AGATACTTTC
    AGCCTTGGGG TTTGCAATGG GTGCCAGCTG ATGGCTCTTC TGGGCTGGGT AGGACCCGAT
    AACCCAACAG ACACAGGTGA TCTCCCCACC CAAGGGGTTC TGCTCAGCCA CAATCTCTCC
    GGCCGGTTTG AATCTCGATT TGTCACCCTG AAGATCCAAC AGAGTCCTTC CATTTTGCTA
    CGAGGGATGG CCGGGTCCAC GCTCGGAGTG TGGGTGGCCC ATGGCGAAGG ATACATGAGG
    TTCCGATCTC CGAAAGTGCA GGACTATGTG ACGTCCAATC ACTTGGCGCC GCTCTGTTAC
    GTGGATGATG GAGGCAAGCC GACGGAAGAA TACCCAATGA ATCCAAATGG CTCTCCGTTG
    GGAATTGCTG GTCTTTGCTC TGCAGATGGG CGGCACTTGG CCATGATGCC CCACCCAGAG
    CGCTGTGTCA TGAAGTGGCA GTGGCCTTGG ATGCCGGAAA GCTGGCGCCA CTCATTGGAT
    GTCTCGCCGT GGATGCGTCT CTTCCAGAAT GGGTACCGCT GGTGCCAGGA GAACCCAAGC
    AGGGATAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa02847
    Clone ID Related Accession (Same CDS sequence) NM_001142220.2
    Accession Version NM_001142220.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3975bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2013-04-17
    Organism Xenopus tropicalis(Tropical clawed frog)
    Product phosphoribosylformylglycinamidine synthase
    Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAMC01096052.1. On Sep 25, 2010 this sequence version replaced NM_001142220.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC168641.1 [ECO:0000332] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGTGGTCC TGCACTTCTA TCGCTCCTTG CAGAATTCAT CAGAGACCCT GCGGCTGCCC 
    GGTGCGGAGA ATATACAGAG CCTGCAGAGG GAGCTGTGCT ACAATGTCAA TTGGACAGGT
    CCCGCGGCCC CATCCAGCCA GGAGACTGAC GCCCTTCGAT GGCTGTTTAG TTGCCCCTTT
    GACCCTCAGA GTATCTCTGA CACCTCATCC CTTCACTCGG AGCCTTCTGA CCTGCTGGTA
    GAGATCGGCC CAAGACTGAA TTTCTCCACC GCGACTTCCA CCAATGCTGT GTCCATCTGC
    CGCTCTGTTG GCCTGACCAA CGTGGACAGG ATCGAGTGCT CCAAGAGGTA TCTCATCAAG
    TTCCAGACTA GGCCCCAGGG CAGTGAGGAG CAAGAGCTGC TAGCCTCTCT GTACGACCGT
    ATGACGGAAT GCGTTTACCC GGAGCCGGTG AGGAGTTTCG AGGTGTCCGT TAGACCGGAG
    AAAGTGTACG AGGTGGATAT TTTGGGGCAA GGCAGGGCAG CACTGGAAAA GGCCAACACT
    GAGCTAGGTT TGGCCTTCGA CTCCTGGGAT CTTGATTACT ATAGCAGCCT TTTCCAGCGC
    GTGGGTCGGA ACCCCAGCAG CGTGGAGTGC TTTGATCTTG CACAGTCCAA CAGCGAGCAC
    AGCCGCCACT GGTTCTTTAA GGGCAAATTA AAGGTCGACG GGCAGGAGAA GGAGATGTCT
    CTGTTCGACA TGATCATGAA GACTCAGGAC ACCAGTAATC CCAACAACGT CATCAAGTTC
    TGTGACAACA GCAGTGCGAT CCAGGGAAGA GAAGTGGCAT CTCTAATCCC AACAGACCCT
    TCATGTGCTG GGCCCTATCA TCTCTGTCGC TCCACACGGC ACCTGATTTT CACTGCAGAG
    ACCCACAACT TCCCTACTGG CGTGGCCCCA TTCAGCGGCG CTACGACAGG AACAGGAGGG
    CGCATTCGGG ACGTGCAGAG CACCGGCAGG GGAGCACATG TCATTGCTGG GACGGCTGGG
    TACTGCTTTG GGAACCTGCA CATCCCAGGA TACTCTCTTC CCTGGGAGGA CCCCGCATAT
    CAGTACCCAG TGCAGTTTGC CAGGCCTCTG GAGGTGGCTA TTGAAGCCAG TAACGGAGCC
    TCTGATTATG GAAACAAGTT TGGGGAGCCT GTTTTGGCCG GATTTGCCCG TTCCTTTGGA
    GTGCGGTTGC CTTCGGGAGA AAGGAGGGAG TGGGTGAAAC CCATTATGTT CAGTGGTGGG
    ATCGGATCCA TGGAGGATGT TCACCGTACT AAGGAGGCCC CAGAGCCTGG CATGCACGTG
    GTTAAAGTGG GAGGTCCTGT CTATAGGATT GGGGTTGGCG GTGGAGCTGC CTCTTCTATT
    CAGGTCCAGG GGGATAACGC CAGCGAGCTG GATTTTGGGG CAGTGCAACG TGGAGATGCA
    GAGATGGAGC AGAAGATGAA TCGGGCCGTA CGGGCCTGCG TGGAGCGAGG AGGCAAGAAC
    CCCATCTGCA GCATTCACGA TCAGGGAGCC GGGGGCAACG GGAACGTCCT GAAGGAGCTC
    AGTGAGCCCC AAGGAGCAGT CATTTACACT AAAAGCTTCC AGCTGGGGGA CCCCACCCTG
    AGTGTTCTGG AGATATGGGG AGCAGAATAT CAGGAGTCCA ACGCTTTGCT CCTGCGCCCC
    GACGATGCCG AATTCCTGCG CTCTGTGTGC CGGCGGGAGA GGTCTCCTGT GGATTTTGTG
    GGCAAGATCA CCGGGGATGG CAGGATTGTG TTGATAAATG GCAGTGACAC AGATCCCGCC
    CCTGACTCCA CTGACCGCAA TGCTGTTCCC GTTGACCTCC AGCTGGAATG GGTCTTAGGA
    AAAATGCCTC GCAAGGAGTT TGTTCTAAAC CGCGTGGCAC CAAACTTGCA GCCGCTGGCG
    CTCCCTAGTG GCCTGACAGT CGGGCAGGCG CTTGATCGCG TGCTGAGGTT GCCGTCCGTA
    GCGAGCAAAC GCTACCTGAC CAACAAGGTG GACCGCTCCG TCACAGGCCT GGTGGCCCAG
    CAGCAGTGCG TGGGGCCTCT TCACACTCCA TTGGCCGACG TGGCTGTGGT GTCTCTGTCT
    TATTCGGACA TTGTAGGAGG AGCTACAGCA ATAGGAGAAC AGCCAATCAA AAGCCTACTG
    AATCCTGCAG CTGGGGCAAG ACTGGCGGTG GCCGAGGCAC TAACCAATCT CCTCTTTGCT
    CAAGTGACGG ACCTAAAGGA TGTGAAATGC AGCGGGAACT GGATGTGGGC CGCCAAGCTG
    CCCGGAGAAG GCGCCTTGCT TTACGACGCC TGTGCAGCTA TGTGTGATGT CATGGCGCAG
    CTCGGCGTTG CCATCGATGG AGGAAAAGAT TCTCTCAGCA TGGCCGCCAG AGTGGGCACA
    GAAACAGTTA AAGCTCCAGG TTCGCTAGTG ATCTCGGTGT ATGCAGTCTG CCCCGACATA
    ACTACCACCG TGACCCCCGA TCTTAAGAAC CCAGGGCAGA AAGGTGTTTT ACTGTATTTG
    CCCCTCTGCC CTGGTCAGCA CCGGCTTGGG GGCAGCGCCC TGGCTCAGTG CTACTCACAG
    CTGGGAGAGA CCCCCCCAGA CCTCGATGAC CCTCAGACTT TAATCTCCTG CTTCAACGTC
    ACCCAACAGC TGCTGAGAGA CCGCGTCCTT AGCGCCGGCC ATGACGTCAG TGATGGAGGC
    CTAGTCACCT GTATCCTGGA AATGGCTTTT GCTGGAAACT GTGGCCTGGA TATTGAGATT
    TCCTCCTCCT GCACCAATGT GCTGGAACTG CTGTTTGCCG AGGAACCGGG CCTGGTTCTA
    GAGGTTGCAG AACAAAGTGT AGAACTGGTC ATGGAACGTT ACAGAGCAGC AGGAGTGGAA
    TGCGTGAGGA TCGGATGCAC CAAGGAACGT GGCCCAGCGT CTATGGTCCG AATCCGTGCC
    AATGGGCAGG AGGTGGTCAA CGAGAAGTTG GGTTCCCTCA GAGCCGTATG GGAGGAGACC
    AGTTTCCAAC TTGAACGCCT TCAGGCCAAT CCAAGCTGTG TATCGCAGGA GGAGGCTGGG
    CTGAGAGTGA GGGAGGGGCC AAGTTATCAC CTGACTTTTA ACCCGTCTGA AATACCTTTG
    GTTCCTCCAT CAGTCGGTGG CAACCAACCG CGTGTTGCTG TTGTGCGTGA AGAGGGTAGC
    AACGGAGACC GGGAAATGGC GGCATCTCTC CTAATGGCTG GATTTCAGGT GTGGGATGTC
    ACCATGGAAG ATCTTCTTGC TGGAGGCACC ACCCTGGACT CTTTCAGGGG ACTTGTATTT
    GTTGGGGGAT TTAGCTACGC TGATGTGTTA GGGTCTGCCA AAGGTTGGGC CGCCTCGGTG
    AAGTTCAATG CCAGCGTGCG CGAGCAGTTC GAGAACTTCC GCCGTAGGGC AGATACTTTC
    AGCCTTGGGG TTTGCAATGG GTGCCAGCTG ATGGCTCTTC TGGGCTGGGT AGGACCCGAT
    AACCCAACAG ACACAGTAGG TGATCTCCCC ACCCAAGGGG TTCTGCTCAG CCACAATCTC
    TCCGGCCGGT TTGAATCTCG ATTTGTCACC CTGAAGATCC AACAGAGTCC TTCCATTTTG
    CTACGAGGGA TGGCCGGGTC CACGCTCGGA GTGTGGGTGG CCCATGGCGA AGGATACATG
    AGGTTCCGAT CTCCGAAAGT GCAGGACTAT GTGACGTCCA ATCACTTGGC GCCGCTCTGT
    TACGTGGATG ATGGAGGCAA GCCGACGGAA GAATACCCAA TGAATCCAAA TGGCTCTCCG
    TTGGGAATTG CTGGTCTTTG CTCTGCAGAT GGGCGGCACT TGGCCATGAT GCCCCACCCA
    GAGCGCTGTG TCATGAAGTG GCAGTGGCCT TGGATGCCGG AAAGCTGGCG CCACTCATTG
    GATGTCTCGC CGTGGATGCG TCTCTTCCAG AATGGGTACC GCTGGTGCCA GGAGAACCCA
    AGCAGGGATA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001135692.2
    CDS46..4020
    Translation

    Target ORF information:

    RefSeq Version NM_001142220.2
    Organism Xenopus tropicalis(Tropical clawed frog)
    Definition Xenopus (Silurana) tropicalis phosphoribosylformylglycinamidine synthase (pfas), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001142220.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGTGGTCC TGCACTTCTA TCGCTCCTTG CAGAATTCAT CAGAGACCCT GCGGCTGCCC 
    GGTGCGGAGA ATATACAGAG CCTGCAGAGG GAGCTGTGCT ACAATGTCAA TTGGACAGGT
    CCCGCGGCCC CATCCAGCCA GGAGACTGAC GCCCTTCGAT GGCTGTTTAG TTGCCCCTTT
    GACCCTCAGA GTATCTCTGA CACCTCATCC CTTCACTCGG AGCCTTCTGA CCTGCTGGTA
    GAGATCGGCC CAAGACTGAA TTTCTCCACC GCGACTTCCA CCAATGCTGT GTCCATCTGC
    CGCTCTGTTG GCCTGACCAA CGTGGACAGG ATCGAGTGCT CCAAGAGGTA TCTCATCAAG
    TTCCAGACTA GGCCCCAGGG CAGTGAGGAG CAAGAGCTGC TAGCCTCTCT GTACGACCGT
    ATGACGGAAT GCGTTTACCC GGAGCCGGTG AGGAGTTTCG AGGTGTCCGT TAGACCGGAG
    AAAGTGTACG AGGTGGATAT TTTGGGGCAA GGCAGGGCAG CACTGGAAAA GGCCAACACT
    GAGCTAGGTT TGGCCTTCGA CTCCTGGGAT CTTGATTACT ATAGCAGCCT TTTCCAGCGC
    GTGGGTCGGA ACCCCAGCAG CGTGGAGTGC TTTGATCTTG CACAGTCCAA CAGCGAGCAC
    AGCCGCCACT GGTTCTTTAA GGGCAAATTA AAGGTCGACG GGCAGGAGAA GGAGATGTCT
    CTGTTCGACA TGATCATGAA GACTCAGGAC ACCAGTAATC CCAACAACGT CATCAAGTTC
    TGTGACAACA GCAGTGCGAT CCAGGGAAGA GAAGTGGCAT CTCTAATCCC AACAGACCCT
    TCATGTGCTG GGCCCTATCA TCTCTGTCGC TCCACACGGC ACCTGATTTT CACTGCAGAG
    ACCCACAACT TCCCTACTGG CGTGGCCCCA TTCAGCGGCG CTACGACAGG AACAGGAGGG
    CGCATTCGGG ACGTGCAGAG CACCGGCAGG GGAGCACATG TCATTGCTGG GACGGCTGGG
    TACTGCTTTG GGAACCTGCA CATCCCAGGA TACTCTCTTC CCTGGGAGGA CCCCGCATAT
    CAGTACCCAG TGCAGTTTGC CAGGCCTCTG GAGGTGGCTA TTGAAGCCAG TAACGGAGCC
    TCTGATTATG GAAACAAGTT TGGGGAGCCT GTTTTGGCCG GATTTGCCCG TTCCTTTGGA
    GTGCGGTTGC CTTCGGGAGA AAGGAGGGAG TGGGTGAAAC CCATTATGTT CAGTGGTGGG
    ATCGGATCCA TGGAGGATGT TCACCGTACT AAGGAGGCCC CAGAGCCTGG CATGCACGTG
    GTTAAAGTGG GAGGTCCTGT CTATAGGATT GGGGTTGGCG GTGGAGCTGC CTCTTCTATT
    CAGGTCCAGG GGGATAACGC CAGCGAGCTG GATTTTGGGG CAGTGCAACG TGGAGATGCA
    GAGATGGAGC AGAAGATGAA TCGGGCCGTA CGGGCCTGCG TGGAGCGAGG AGGCAAGAAC
    CCCATCTGCA GCATTCACGA TCAGGGAGCC GGGGGCAACG GGAACGTCCT GAAGGAGCTC
    AGTGAGCCCC AAGGAGCAGT CATTTACACT AAAAGCTTCC AGCTGGGGGA CCCCACCCTG
    AGTGTTCTGG AGATATGGGG AGCAGAATAT CAGGAGTCCA ACGCTTTGCT CCTGCGCCCC
    GACGATGCCG AATTCCTGCG CTCTGTGTGC CGGCGGGAGA GGTCTCCTGT GGATTTTGTG
    GGCAAGATCA CCGGGGATGG CAGGATTGTG TTGATAAATG GCAGTGACAC AGATCCCGCC
    CCTGACTCCA CTGACCGCAA TGCTGTTCCC GTTGACCTCC AGCTGGAATG GGTCTTAGGA
    AAAATGCCTC GCAAGGAGTT TGTTCTAAAC CGCGTGGCAC CAAACTTGCA GCCGCTGGCG
    CTCCCTAGTG GCCTGACAGT CGGGCAGGCG CTTGATCGCG TGCTGAGGTT GCCGTCCGTA
    GCGAGCAAAC GCTACCTGAC CAACAAGGTG GACCGCTCCG TCACAGGCCT GGTGGCCCAG
    CAGCAGTGCG TGGGGCCTCT TCACACTCCA TTGGCCGACG TGGCTGTGGT GTCTCTGTCT
    TATTCGGACA TTGTAGGAGG AGCTACAGCA ATAGGAGAAC AGCCAATCAA AAGCCTACTG
    AATCCTGCAG CTGGGGCAAG ACTGGCGGTG GCCGAGGCAC TAACCAATCT CCTCTTTGCT
    CAAGTGACGG ACCTAAAGGA TGTGAAATGC AGCGGGAACT GGATGTGGGC CGCCAAGCTG
    CCCGGAGAAG GCGCCTTGCT TTACGACGCC TGTGCAGCTA TGTGTGATGT CATGGCGCAG
    CTCGGCGTTG CCATCGATGG AGGAAAAGAT TCTCTCAGCA TGGCCGCCAG AGTGGGCACA
    GAAACAGTTA AAGCTCCAGG TTCGCTAGTG ATCTCGGTGT ATGCAGTCTG CCCCGACATA
    ACTACCACCG TGACCCCCGA TCTTAAGAAC CCAGGGCAGA AAGGTGTTTT ACTGTATTTG
    CCCCTCTGCC CTGGTCAGCA CCGGCTTGGG GGCAGCGCCC TGGCTCAGTG CTACTCACAG
    CTGGGAGAGA CCCCCCCAGA CCTCGATGAC CCTCAGACTT TAATCTCCTG CTTCAACGTC
    ACCCAACAGC TGCTGAGAGA CCGCGTCCTT AGCGCCGGCC ATGACGTCAG TGATGGAGGC
    CTAGTCACCT GTATCCTGGA AATGGCTTTT GCTGGAAACT GTGGCCTGGA TATTGAGATT
    TCCTCCTCCT GCACCAATGT GCTGGAACTG CTGTTTGCCG AGGAACCGGG CCTGGTTCTA
    GAGGTTGCAG AACAAAGTGT AGAACTGGTC ATGGAACGTT ACAGAGCAGC AGGAGTGGAA
    TGCGTGAGGA TCGGATGCAC CAAGGAACGT GGCCCAGCGT CTATGGTCCG AATCCGTGCC
    AATGGGCAGG AGGTGGTCAA CGAGAAGTTG GGTTCCCTCA GAGCCGTATG GGAGGAGACC
    AGTTTCCAAC TTGAACGCCT TCAGGCCAAT CCAAGCTGTG TATCGCAGGA GGAGGCTGGG
    CTGAGAGTGA GGGAGGGGCC AAGTTATCAC CTGACTTTTA ACCCGTCTGA AATACCTTTG
    GTTCCTCCAT CAGTCGGTGG CAACCAACCG CGTGTTGCTG TTGTGCGTGA AGAGGGTAGC
    AACGGAGACC GGGAAATGGC GGCATCTCTC CTAATGGCTG GATTTCAGGT GTGGGATGTC
    ACCATGGAAG ATCTTCTTGC TGGAGGCACC ACCCTGGACT CTTTCAGGGG ACTTGTATTT
    GTTGGGGGAT TTAGCTACGC TGATGTGTTA GGGTCTGCCA AAGGTTGGGC CGCCTCGGTG
    AAGTTCAATG CCAGCGTGCG CGAGCAGTTC GAGAACTTCC GCCGTAGGGC AGATACTTTC
    AGCCTTGGGG TTTGCAATGG GTGCCAGCTG ATGGCTCTTC TGGGCTGGGT AGGACCCGAT
    AACCCAACAG ACACAGTAGG TGATCTCCCC ACCCAAGGGG TTCTGCTCAG CCACAATCTC
    TCCGGCCGGT TTGAATCTCG ATTTGTCACC CTGAAGATCC AACAGAGTCC TTCCATTTTG
    CTACGAGGGA TGGCCGGGTC CACGCTCGGA GTGTGGGTGG CCCATGGCGA AGGATACATG
    AGGTTCCGAT CTCCGAAAGT GCAGGACTAT GTGACGTCCA ATCACTTGGC GCCGCTCTGT
    TACGTGGATG ATGGAGGCAA GCCGACGGAA GAATACCCAA TGAATCCAAA TGGCTCTCCG
    TTGGGAATTG CTGGTCTTTG CTCTGCAGAT GGGCGGCACT TGGCCATGAT GCCCCACCCA
    GAGCGCTGTG TCATGAAGTG GCAGTGGCCT TGGATGCCGG AAAGCTGGCG CCACTCATTG
    GATGTCTCGC CGTGGATGCG TCTCTTCCAG AATGGGTACC GCTGGTGCCA GGAGAACCCA
    AGCAGGGATA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32769
    Clone ID Related Accession (Same CDS sequence) NM_001142220.3 , XM_012970190.2 , XM_012970191.2
    Accession Version NM_001142220.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3975bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-07-06
    Organism Xenopus tropicalis(tropical clawed frog)
    Product phosphoribosylformylglycinamidine synthase
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAMC03033817.1. On Jul 7, 2017 this sequence version replaced NM_001142220.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC168641.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1025683, SAMEA1025684 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGTGGTCC TGCACTTCTA TCGCTCCTTG CAGAATTCAT CAGAGACCCT GCGGCTGCCC 
    GGTGCGGAGA ATATACAGAG CCTGCAGAGG GAGCTGTGCT ACAATGTCAA TTGGACAGGT
    CCCGCGGCCC CATCCAGCCA GGAGACTGAC GCCCTTCGAT GGCTGTTTAG TTGCCCCTTT
    GACCCTCAGA GTATCTCTGA CACCTCATCC CTTCACTCGG AGCCTTCTGA CCTGCTGGTA
    GAGATCGGCC CAAGACTGAA TTTCTCCACC GCGACTTCCA CCAATGCTGT GTCCATCTGC
    CGCTCTGTTG GCCTGACCAA CGTGGACAGG ATCGAGTGCT CCAAGAGGTA TCTCATCAAG
    TTCCAGACTA GGCCCCAGGG CAGTGAGGAG CAAGAGCTGC TAGCCTCTCT GTACGACCGT
    ATGACGGAAT GCGTTTACCC GGAGCCGGTG AGGAGTTTCG AGGTGTCCGT TAGACCGGAG
    AAAGTGTACG AGGTGGATAT TTTGGGGCAA GGCAGGGCAG CACTGGAAAA GGCCAACACT
    GAGCTAGGTT TGGCCTTCGA CTCCTGGGAT CTTGATTACT ATAGCAGCCT TTTCCAGCGC
    GTGGGTCGGA ACCCCAGCAG CGTGGAGTGC TTTGATCTTG CACAGTCCAA CAGCGAGCAC
    AGCCGCCACT GGTTCTTTAA GGGCAAATTA AAGGTCGACG GGCAGGAGAA GGAGATGTCT
    CTGTTCGACA TGATCATGAA GACTCAGGAC ACCAGTAATC CCAACAACGT CATCAAGTTC
    TGTGACAACA GCAGTGCGAT CCAGGGAAGA GAAGTGGCAT CTCTAATCCC AACAGACCCT
    TCATGTGCTG GGCCCTATCA TCTCTGTCGC TCCACACGGC ACCTGATTTT CACTGCAGAG
    ACCCACAACT TCCCTACTGG CGTGGCCCCA TTCAGCGGCG CTACGACAGG AACAGGAGGG
    CGCATTCGGG ACGTGCAGAG CACCGGCAGG GGAGCACATG TCATTGCTGG GACGGCTGGG
    TACTGCTTTG GGAACCTGCA CATCCCAGGA TACTCTCTTC CCTGGGAGGA CCTCGCATAT
    CAGTACCCAG TGCAGTTTGC CAGGCCTCTG GAGGTGGCTA TTGAAGCCAG TAACGGAGCC
    TCTGATTATG GAAACAAGTT TGGGGAGCCT GTTTTGGCCG GATTTGCCCG TTCCTTTGGC
    CTGCGGTTGC CTTCGGGAGA AAGGAGGGAG TGGGTGAAAC CCATTATGTT CAGTGGTGGG
    ATCGGATCCA TGGAGGATGT TCACCGTACT AAGGAGGCCC CAGAGCCTGG CATGCACGTG
    GTTAAAGTGG GAGGTCCTGT CTATAGGATT GGGGTTGGCG GTGGAGCTGC CTCTTCTATT
    CAGGTCCAGG GGGATAACGC CAGCGAGCTG GATTTTGGGG CAGTGCAACG TGGAGATGCA
    GAGATGGAGC AGAAGATGAA TCGGGCCGTA CGGGCCTGCG TGGAGCGAGG AGGCAAGAAC
    CCCATCTGCA GCATTCACGA TCAGGGAGCC GGGGGCAACG GGAACGTCCT GAAGGAGCTC
    AGTGAGCCCC AAGGAGCAGT CATTTACACT AAAAGCTTCC AGCTGGGGGA CCCCACCCTG
    AGTGTTCTGG AGATATGGGG AGCAGAATAT CAGGAGTCCA ACGCTTTGCT CCTGCGCCCC
    GACGATGCCG AATTCCTGCG CTCTGTGTGC CGGCGGGAGA GGTCTCCTGT GGATTTTGTG
    GGCAAGATCA CCGGGGATGG CAGGATTGTG TTGATAAATG GCAGTGACAC AGATCCCGCC
    CCTGACTCCA CTGACCGCAA TGCTGTTCCC GTTGACCTCC AGCTGGAATG GGTCTTAGGA
    AAAATGCCTC GCAAGGAGTT TGTTCTAAAC CGCGTGGCAC CAAACTTGCA GCCGCTGGTG
    CTCCCTAGTG GCCTGACAGT CGGGCAGGCG CTTGATCGCG TGCTGAGGTT GCCGTCCGTA
    GCGAGCAAAC GCTACCTGAC CAACAAGGTG GACCGCTCCG TCACAGGCCT GGTGGCCCAG
    CAGCAGTGCG TGGGGCCTCT TCACACTCCA TTGGCCGACG TGGCTGTGGT GTCTCTGTCT
    TATTCGGACA TTGTAGGAGG AGCTACAGCA ATAGGAGAAC AGCCAATCAA AAGCCTACTG
    AATCCTGCAG CTGGGGCAAG ACTGGCGGTG GCCGAGGCAC TAACCAATCT CCTCTTTGCT
    CAAGTGACGG ACCTAAAGGA TGTGAAATGC AGCGGGAACT GGATGTGGGC CGCCAAGCTG
    CCCGGAGAAG GCGCCTTGCT TTACGACGCC TGTGCAGCTA TGTGTGATGT CATGGCGCAG
    CTCGGCGTTG CCATCGATGG AGGAAAAGAT TCTCTCAGCA TGGCCGCCAG AGTGGGCACA
    GAAACAGTTA AAGCTCCAGG TTCGCTAGTG ATCTCGGTGT ATGCAGTCTG CCCCGACATA
    ACTACCACCG TGACCCCCGA TCTTAAGAAC CCAGGGCAGA AAGGTGTTTT ACTGTATTTG
    CCCCTCTGCC CTGGTCAGCA CCGGCTTGGG GGCAGCGCCC TGGCTCAGTG CTACTCACAG
    CTGGGAGAGA CCCCCCCAGA CCTCGATGAC CCTCAGACTT TAATCTCCTG CTTCAACGTC
    ACCCAACAGC TGCTGAGAGA CCGCGTCCTT AGCGCCGGCC ATGACGTCAG TGATGGAGGC
    CTAGTCACGT GTATCCTGGA AATGGCTTTT GCTGGAAACT GTGGCCTGGA TATTGAGATT
    TCCTCCTCCT GCACCAATGT GCTGGAACTG CTGTTTGCCG AGGAACCGGG CCTGGTTCTA
    GAGGTTGCAG AACAAAGTGT AGAACTGGTC ATGGAACGTT ACAGAGCAGC AGGAGTGGAA
    TGCGTGAGGA TCGGATGCAC CAAGGAACGT GGCCCAGCGT CTATGGTCCG AATCCGTGCC
    AATGGGCAGG AGGTGGTCAA CGAGAAGTTG GGTTCCCTCA GAGCCGTATG GGAGGAGACC
    AGTTTCCAAC TTGAACGCCT TCAGGCCAAT CCAAGCTGTG TATCGCAGGA GGAGGCTGGG
    CTGAGAGTGA GGGAGGGGCC AAGTTATCAC CTGACTTTTA ACCCGTCTGA AATACCTTTG
    GTTCCTCCAT CAGTCGGTGG CAACCAACCG CGTGTTGCTG TTGTGCGTGA AGAGGGTAGC
    AACGGAGACC GGGAAATGGC GGCATCTCTC CTAATGGCTG GATTTCAGGT GTGGGATGTC
    ACCATGGAAG ATCTTCTTGC TGGAGGCACC ACCCTGGACT CTTTCAGGGG ACTTGTATTT
    GTTGGGGGAT TTAGCTACGC TGATGTGTTA GGGTCTGCCA AAGGTTGGGC CGCCTCGGTG
    AAGTTCAATG CCAGCGTGCG CGAGCAGTTC GAGAACTTCC GCCGTAGGGC AGATACTTTC
    AGCCTTGGGG TTTGCAATGG GTGCCAGCTG ATGGCTCTTC TGGGCTGGGT AGGACCCGAT
    AACCCAACAG ACACAGTAGG TGATCTCCCC ACCCAAGGGG TTCTGCTCAG CCACAATCTC
    TCCGGCCGGT TTGAATCTCG ATTTGTCACC CTGAAGATCC AACAGAGTCC TTCCATTTTG
    CTACGAGGGA TGGCCGGGTC CACGCTCGGA GTGTGGGTGG CCCATGGCGA AGGATACATG
    AGGTTCCGAT CTCCGAAAGT GCAGGACTAT GTGACGTCCA ATCACTTGGC GCCGCTCTGT
    TACGTGGATG ATGGAGGCAA GCCGACGGAA GAATACCCAA TGAATCCAAA TGGCTCTCCG
    TTGGGAATTG CTGGTCTTTG CTCTGCAGAT GGGCGGCACT TGGCCATGAT GCCCCACCCA
    GAGCGCTGTG TCATGAAGTG GCAGTGGCCT TGGATGCCGG AAAGCTGGCG CCACTCATTG
    GATGTCTCGC CGTGGATGCG TCTCTTCCAG AATGGGTACC GCTGGTGCCA GGAGAACCCA
    AGCAGGGATA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001135692.3
    CDS46..4020
    Translation

    Target ORF information:

    RefSeq Version NM_001142220.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phosphoribosylformylglycinamidine synthase (pfas), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001142220.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGTGGTCC TGCACTTCTA TCGCTCCTTG CAGAATTCAT CAGAGACCCT GCGGCTGCCC 
    GGTGCGGAGA ATATACAGAG CCTGCAGAGG GAGCTGTGCT ACAATGTCAA TTGGACAGGT
    CCCGCGGCCC CATCCAGCCA GGAGACTGAC GCCCTTCGAT GGCTGTTTAG TTGCCCCTTT
    GACCCTCAGA GTATCTCTGA CACCTCATCC CTTCACTCGG AGCCTTCTGA CCTGCTGGTA
    GAGATCGGCC CAAGACTGAA TTTCTCCACC GCGACTTCCA CCAATGCTGT GTCCATCTGC
    CGCTCTGTTG GCCTGACCAA CGTGGACAGG ATCGAGTGCT CCAAGAGGTA TCTCATCAAG
    TTCCAGACTA GGCCCCAGGG CAGTGAGGAG CAAGAGCTGC TAGCCTCTCT GTACGACCGT
    ATGACGGAAT GCGTTTACCC GGAGCCGGTG AGGAGTTTCG AGGTGTCCGT TAGACCGGAG
    AAAGTGTACG AGGTGGATAT TTTGGGGCAA GGCAGGGCAG CACTGGAAAA GGCCAACACT
    GAGCTAGGTT TGGCCTTCGA CTCCTGGGAT CTTGATTACT ATAGCAGCCT TTTCCAGCGC
    GTGGGTCGGA ACCCCAGCAG CGTGGAGTGC TTTGATCTTG CACAGTCCAA CAGCGAGCAC
    AGCCGCCACT GGTTCTTTAA GGGCAAATTA AAGGTCGACG GGCAGGAGAA GGAGATGTCT
    CTGTTCGACA TGATCATGAA GACTCAGGAC ACCAGTAATC CCAACAACGT CATCAAGTTC
    TGTGACAACA GCAGTGCGAT CCAGGGAAGA GAAGTGGCAT CTCTAATCCC AACAGACCCT
    TCATGTGCTG GGCCCTATCA TCTCTGTCGC TCCACACGGC ACCTGATTTT CACTGCAGAG
    ACCCACAACT TCCCTACTGG CGTGGCCCCA TTCAGCGGCG CTACGACAGG AACAGGAGGG
    CGCATTCGGG ACGTGCAGAG CACCGGCAGG GGAGCACATG TCATTGCTGG GACGGCTGGG
    TACTGCTTTG GGAACCTGCA CATCCCAGGA TACTCTCTTC CCTGGGAGGA CCTCGCATAT
    CAGTACCCAG TGCAGTTTGC CAGGCCTCTG GAGGTGGCTA TTGAAGCCAG TAACGGAGCC
    TCTGATTATG GAAACAAGTT TGGGGAGCCT GTTTTGGCCG GATTTGCCCG TTCCTTTGGC
    CTGCGGTTGC CTTCGGGAGA AAGGAGGGAG TGGGTGAAAC CCATTATGTT CAGTGGTGGG
    ATCGGATCCA TGGAGGATGT TCACCGTACT AAGGAGGCCC CAGAGCCTGG CATGCACGTG
    GTTAAAGTGG GAGGTCCTGT CTATAGGATT GGGGTTGGCG GTGGAGCTGC CTCTTCTATT
    CAGGTCCAGG GGGATAACGC CAGCGAGCTG GATTTTGGGG CAGTGCAACG TGGAGATGCA
    GAGATGGAGC AGAAGATGAA TCGGGCCGTA CGGGCCTGCG TGGAGCGAGG AGGCAAGAAC
    CCCATCTGCA GCATTCACGA TCAGGGAGCC GGGGGCAACG GGAACGTCCT GAAGGAGCTC
    AGTGAGCCCC AAGGAGCAGT CATTTACACT AAAAGCTTCC AGCTGGGGGA CCCCACCCTG
    AGTGTTCTGG AGATATGGGG AGCAGAATAT CAGGAGTCCA ACGCTTTGCT CCTGCGCCCC
    GACGATGCCG AATTCCTGCG CTCTGTGTGC CGGCGGGAGA GGTCTCCTGT GGATTTTGTG
    GGCAAGATCA CCGGGGATGG CAGGATTGTG TTGATAAATG GCAGTGACAC AGATCCCGCC
    CCTGACTCCA CTGACCGCAA TGCTGTTCCC GTTGACCTCC AGCTGGAATG GGTCTTAGGA
    AAAATGCCTC GCAAGGAGTT TGTTCTAAAC CGCGTGGCAC CAAACTTGCA GCCGCTGGTG
    CTCCCTAGTG GCCTGACAGT CGGGCAGGCG CTTGATCGCG TGCTGAGGTT GCCGTCCGTA
    GCGAGCAAAC GCTACCTGAC CAACAAGGTG GACCGCTCCG TCACAGGCCT GGTGGCCCAG
    CAGCAGTGCG TGGGGCCTCT TCACACTCCA TTGGCCGACG TGGCTGTGGT GTCTCTGTCT
    TATTCGGACA TTGTAGGAGG AGCTACAGCA ATAGGAGAAC AGCCAATCAA AAGCCTACTG
    AATCCTGCAG CTGGGGCAAG ACTGGCGGTG GCCGAGGCAC TAACCAATCT CCTCTTTGCT
    CAAGTGACGG ACCTAAAGGA TGTGAAATGC AGCGGGAACT GGATGTGGGC CGCCAAGCTG
    CCCGGAGAAG GCGCCTTGCT TTACGACGCC TGTGCAGCTA TGTGTGATGT CATGGCGCAG
    CTCGGCGTTG CCATCGATGG AGGAAAAGAT TCTCTCAGCA TGGCCGCCAG AGTGGGCACA
    GAAACAGTTA AAGCTCCAGG TTCGCTAGTG ATCTCGGTGT ATGCAGTCTG CCCCGACATA
    ACTACCACCG TGACCCCCGA TCTTAAGAAC CCAGGGCAGA AAGGTGTTTT ACTGTATTTG
    CCCCTCTGCC CTGGTCAGCA CCGGCTTGGG GGCAGCGCCC TGGCTCAGTG CTACTCACAG
    CTGGGAGAGA CCCCCCCAGA CCTCGATGAC CCTCAGACTT TAATCTCCTG CTTCAACGTC
    ACCCAACAGC TGCTGAGAGA CCGCGTCCTT AGCGCCGGCC ATGACGTCAG TGATGGAGGC
    CTAGTCACGT GTATCCTGGA AATGGCTTTT GCTGGAAACT GTGGCCTGGA TATTGAGATT
    TCCTCCTCCT GCACCAATGT GCTGGAACTG CTGTTTGCCG AGGAACCGGG CCTGGTTCTA
    GAGGTTGCAG AACAAAGTGT AGAACTGGTC ATGGAACGTT ACAGAGCAGC AGGAGTGGAA
    TGCGTGAGGA TCGGATGCAC CAAGGAACGT GGCCCAGCGT CTATGGTCCG AATCCGTGCC
    AATGGGCAGG AGGTGGTCAA CGAGAAGTTG GGTTCCCTCA GAGCCGTATG GGAGGAGACC
    AGTTTCCAAC TTGAACGCCT TCAGGCCAAT CCAAGCTGTG TATCGCAGGA GGAGGCTGGG
    CTGAGAGTGA GGGAGGGGCC AAGTTATCAC CTGACTTTTA ACCCGTCTGA AATACCTTTG
    GTTCCTCCAT CAGTCGGTGG CAACCAACCG CGTGTTGCTG TTGTGCGTGA AGAGGGTAGC
    AACGGAGACC GGGAAATGGC GGCATCTCTC CTAATGGCTG GATTTCAGGT GTGGGATGTC
    ACCATGGAAG ATCTTCTTGC TGGAGGCACC ACCCTGGACT CTTTCAGGGG ACTTGTATTT
    GTTGGGGGAT TTAGCTACGC TGATGTGTTA GGGTCTGCCA AAGGTTGGGC CGCCTCGGTG
    AAGTTCAATG CCAGCGTGCG CGAGCAGTTC GAGAACTTCC GCCGTAGGGC AGATACTTTC
    AGCCTTGGGG TTTGCAATGG GTGCCAGCTG ATGGCTCTTC TGGGCTGGGT AGGACCCGAT
    AACCCAACAG ACACAGTAGG TGATCTCCCC ACCCAAGGGG TTCTGCTCAG CCACAATCTC
    TCCGGCCGGT TTGAATCTCG ATTTGTCACC CTGAAGATCC AACAGAGTCC TTCCATTTTG
    CTACGAGGGA TGGCCGGGTC CACGCTCGGA GTGTGGGTGG CCCATGGCGA AGGATACATG
    AGGTTCCGAT CTCCGAAAGT GCAGGACTAT GTGACGTCCA ATCACTTGGC GCCGCTCTGT
    TACGTGGATG ATGGAGGCAA GCCGACGGAA GAATACCCAA TGAATCCAAA TGGCTCTCCG
    TTGGGAATTG CTGGTCTTTG CTCTGCAGAT GGGCGGCACT TGGCCATGAT GCCCCACCCA
    GAGCGCTGTG TCATGAAGTG GCAGTGGCCT TGGATGCCGG AAAGCTGGCG CCACTCATTG
    GATGTCTCGC CGTGGATGCG TCTCTTCCAG AATGGGTACC GCTGGTGCCA GGAGAACCCA
    AGCAGGGATA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32769
    Clone ID Related Accession (Same CDS sequence) NM_001142220.3 , XM_012970190.2 , XM_012970191.2
    Accession Version XM_012970190.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3975bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product phosphoribosylformylglycinamidine synthase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970190.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGTGGTCC TGCACTTCTA TCGCTCCTTG CAGAATTCAT CAGAGACCCT GCGGCTGCCC 
    GGTGCGGAGA ATATACAGAG CCTGCAGAGG GAGCTGTGCT ACAATGTCAA TTGGACAGGT
    CCCGCGGCCC CATCCAGCCA GGAGACTGAC GCCCTTCGAT GGCTGTTTAG TTGCCCCTTT
    GACCCTCAGA GTATCTCTGA CACCTCATCC CTTCACTCGG AGCCTTCTGA CCTGCTGGTA
    GAGATCGGCC CAAGACTGAA TTTCTCCACC GCGACTTCCA CCAATGCTGT GTCCATCTGC
    CGCTCTGTTG GCCTGACCAA CGTGGACAGG ATCGAGTGCT CCAAGAGGTA TCTCATCAAG
    TTCCAGACTA GGCCCCAGGG CAGTGAGGAG CAAGAGCTGC TAGCCTCTCT GTACGACCGT
    ATGACGGAAT GCGTTTACCC GGAGCCGGTG AGGAGTTTCG AGGTGTCCGT TAGACCGGAG
    AAAGTGTACG AGGTGGATAT TTTGGGGCAA GGCAGGGCAG CACTGGAAAA GGCCAACACT
    GAGCTAGGTT TGGCCTTCGA CTCCTGGGAT CTTGATTACT ATAGCAGCCT TTTCCAGCGC
    GTGGGTCGGA ACCCCAGCAG CGTGGAGTGC TTTGATCTTG CACAGTCCAA CAGCGAGCAC
    AGCCGCCACT GGTTCTTTAA GGGCAAATTA AAGGTCGACG GGCAGGAGAA GGAGATGTCT
    CTGTTCGACA TGATCATGAA GACTCAGGAC ACCAGTAATC CCAACAACGT CATCAAGTTC
    TGTGACAACA GCAGTGCGAT CCAGGGAAGA GAAGTGGCAT CTCTAATCCC AACAGACCCT
    TCATGTGCTG GGCCCTATCA TCTCTGTCGC TCCACACGGC ACCTGATTTT CACTGCAGAG
    ACCCACAACT TCCCTACTGG CGTGGCCCCA TTCAGCGGCG CTACGACAGG AACAGGAGGG
    CGCATTCGGG ACGTGCAGAG CACCGGCAGG GGAGCACATG TCATTGCTGG GACGGCTGGG
    TACTGCTTTG GGAACCTGCA CATCCCAGGA TACTCTCTTC CCTGGGAGGA CCTCGCATAT
    CAGTACCCAG TGCAGTTTGC CAGGCCTCTG GAGGTGGCTA TTGAAGCCAG TAACGGAGCC
    TCTGATTATG GAAACAAGTT TGGGGAGCCT GTTTTGGCCG GATTTGCCCG TTCCTTTGGC
    CTGCGGTTGC CTTCGGGAGA AAGGAGGGAG TGGGTGAAAC CCATTATGTT CAGTGGTGGG
    ATCGGATCCA TGGAGGATGT TCACCGTACT AAGGAGGCCC CAGAGCCTGG CATGCACGTG
    GTTAAAGTGG GAGGTCCTGT CTATAGGATT GGGGTTGGCG GTGGAGCTGC CTCTTCTATT
    CAGGTCCAGG GGGATAACGC CAGCGAGCTG GATTTTGGGG CAGTGCAACG TGGAGATGCA
    GAGATGGAGC AGAAGATGAA TCGGGCCGTA CGGGCCTGCG TGGAGCGAGG AGGCAAGAAC
    CCCATCTGCA GCATTCACGA TCAGGGAGCC GGGGGCAACG GGAACGTCCT GAAGGAGCTC
    AGTGAGCCCC AAGGAGCAGT CATTTACACT AAAAGCTTCC AGCTGGGGGA CCCCACCCTG
    AGTGTTCTGG AGATATGGGG AGCAGAATAT CAGGAGTCCA ACGCTTTGCT CCTGCGCCCC
    GACGATGCCG AATTCCTGCG CTCTGTGTGC CGGCGGGAGA GGTCTCCTGT GGATTTTGTG
    GGCAAGATCA CCGGGGATGG CAGGATTGTG TTGATAAATG GCAGTGACAC AGATCCCGCC
    CCTGACTCCA CTGACCGCAA TGCTGTTCCC GTTGACCTCC AGCTGGAATG GGTCTTAGGA
    AAAATGCCTC GCAAGGAGTT TGTTCTAAAC CGCGTGGCAC CAAACTTGCA GCCGCTGGTG
    CTCCCTAGTG GCCTGACAGT CGGGCAGGCG CTTGATCGCG TGCTGAGGTT GCCGTCCGTA
    GCGAGCAAAC GCTACCTGAC CAACAAGGTG GACCGCTCCG TCACAGGCCT GGTGGCCCAG
    CAGCAGTGCG TGGGGCCTCT TCACACTCCA TTGGCCGACG TGGCTGTGGT GTCTCTGTCT
    TATTCGGACA TTGTAGGAGG AGCTACAGCA ATAGGAGAAC AGCCAATCAA AAGCCTACTG
    AATCCTGCAG CTGGGGCAAG ACTGGCGGTG GCCGAGGCAC TAACCAATCT CCTCTTTGCT
    CAAGTGACGG ACCTAAAGGA TGTGAAATGC AGCGGGAACT GGATGTGGGC CGCCAAGCTG
    CCCGGAGAAG GCGCCTTGCT TTACGACGCC TGTGCAGCTA TGTGTGATGT CATGGCGCAG
    CTCGGCGTTG CCATCGATGG AGGAAAAGAT TCTCTCAGCA TGGCCGCCAG AGTGGGCACA
    GAAACAGTTA AAGCTCCAGG TTCGCTAGTG ATCTCGGTGT ATGCAGTCTG CCCCGACATA
    ACTACCACCG TGACCCCCGA TCTTAAGAAC CCAGGGCAGA AAGGTGTTTT ACTGTATTTG
    CCCCTCTGCC CTGGTCAGCA CCGGCTTGGG GGCAGCGCCC TGGCTCAGTG CTACTCACAG
    CTGGGAGAGA CCCCCCCAGA CCTCGATGAC CCTCAGACTT TAATCTCCTG CTTCAACGTC
    ACCCAACAGC TGCTGAGAGA CCGCGTCCTT AGCGCCGGCC ATGACGTCAG TGATGGAGGC
    CTAGTCACGT GTATCCTGGA AATGGCTTTT GCTGGAAACT GTGGCCTGGA TATTGAGATT
    TCCTCCTCCT GCACCAATGT GCTGGAACTG CTGTTTGCCG AGGAACCGGG CCTGGTTCTA
    GAGGTTGCAG AACAAAGTGT AGAACTGGTC ATGGAACGTT ACAGAGCAGC AGGAGTGGAA
    TGCGTGAGGA TCGGATGCAC CAAGGAACGT GGCCCAGCGT CTATGGTCCG AATCCGTGCC
    AATGGGCAGG AGGTGGTCAA CGAGAAGTTG GGTTCCCTCA GAGCCGTATG GGAGGAGACC
    AGTTTCCAAC TTGAACGCCT TCAGGCCAAT CCAAGCTGTG TATCGCAGGA GGAGGCTGGG
    CTGAGAGTGA GGGAGGGGCC AAGTTATCAC CTGACTTTTA ACCCGTCTGA AATACCTTTG
    GTTCCTCCAT CAGTCGGTGG CAACCAACCG CGTGTTGCTG TTGTGCGTGA AGAGGGTAGC
    AACGGAGACC GGGAAATGGC GGCATCTCTC CTAATGGCTG GATTTCAGGT GTGGGATGTC
    ACCATGGAAG ATCTTCTTGC TGGAGGCACC ACCCTGGACT CTTTCAGGGG ACTTGTATTT
    GTTGGGGGAT TTAGCTACGC TGATGTGTTA GGGTCTGCCA AAGGTTGGGC CGCCTCGGTG
    AAGTTCAATG CCAGCGTGCG CGAGCAGTTC GAGAACTTCC GCCGTAGGGC AGATACTTTC
    AGCCTTGGGG TTTGCAATGG GTGCCAGCTG ATGGCTCTTC TGGGCTGGGT AGGACCCGAT
    AACCCAACAG ACACAGTAGG TGATCTCCCC ACCCAAGGGG TTCTGCTCAG CCACAATCTC
    TCCGGCCGGT TTGAATCTCG ATTTGTCACC CTGAAGATCC AACAGAGTCC TTCCATTTTG
    CTACGAGGGA TGGCCGGGTC CACGCTCGGA GTGTGGGTGG CCCATGGCGA AGGATACATG
    AGGTTCCGAT CTCCGAAAGT GCAGGACTAT GTGACGTCCA ATCACTTGGC GCCGCTCTGT
    TACGTGGATG ATGGAGGCAA GCCGACGGAA GAATACCCAA TGAATCCAAA TGGCTCTCCG
    TTGGGAATTG CTGGTCTTTG CTCTGCAGAT GGGCGGCACT TGGCCATGAT GCCCCACCCA
    GAGCGCTGTG TCATGAAGTG GCAGTGGCCT TGGATGCCGG AAAGCTGGCG CCACTCATTG
    GATGTCTCGC CGTGGATGCG TCTCTTCCAG AATGGGTACC GCTGGTGCCA GGAGAACCCA
    AGCAGGGATA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012825644.1
    CDS105..4079
    Translation

    Target ORF information:

    RefSeq Version XM_012970190.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phosphoribosylformylglycinamidine synthase (pfas), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970190.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGTGGTCC TGCACTTCTA TCGCTCCTTG CAGAATTCAT CAGAGACCCT GCGGCTGCCC 
    GGTGCGGAGA ATATACAGAG CCTGCAGAGG GAGCTGTGCT ACAATGTCAA TTGGACAGGT
    CCCGCGGCCC CATCCAGCCA GGAGACTGAC GCCCTTCGAT GGCTGTTTAG TTGCCCCTTT
    GACCCTCAGA GTATCTCTGA CACCTCATCC CTTCACTCGG AGCCTTCTGA CCTGCTGGTA
    GAGATCGGCC CAAGACTGAA TTTCTCCACC GCGACTTCCA CCAATGCTGT GTCCATCTGC
    CGCTCTGTTG GCCTGACCAA CGTGGACAGG ATCGAGTGCT CCAAGAGGTA TCTCATCAAG
    TTCCAGACTA GGCCCCAGGG CAGTGAGGAG CAAGAGCTGC TAGCCTCTCT GTACGACCGT
    ATGACGGAAT GCGTTTACCC GGAGCCGGTG AGGAGTTTCG AGGTGTCCGT TAGACCGGAG
    AAAGTGTACG AGGTGGATAT TTTGGGGCAA GGCAGGGCAG CACTGGAAAA GGCCAACACT
    GAGCTAGGTT TGGCCTTCGA CTCCTGGGAT CTTGATTACT ATAGCAGCCT TTTCCAGCGC
    GTGGGTCGGA ACCCCAGCAG CGTGGAGTGC TTTGATCTTG CACAGTCCAA CAGCGAGCAC
    AGCCGCCACT GGTTCTTTAA GGGCAAATTA AAGGTCGACG GGCAGGAGAA GGAGATGTCT
    CTGTTCGACA TGATCATGAA GACTCAGGAC ACCAGTAATC CCAACAACGT CATCAAGTTC
    TGTGACAACA GCAGTGCGAT CCAGGGAAGA GAAGTGGCAT CTCTAATCCC AACAGACCCT
    TCATGTGCTG GGCCCTATCA TCTCTGTCGC TCCACACGGC ACCTGATTTT CACTGCAGAG
    ACCCACAACT TCCCTACTGG CGTGGCCCCA TTCAGCGGCG CTACGACAGG AACAGGAGGG
    CGCATTCGGG ACGTGCAGAG CACCGGCAGG GGAGCACATG TCATTGCTGG GACGGCTGGG
    TACTGCTTTG GGAACCTGCA CATCCCAGGA TACTCTCTTC CCTGGGAGGA CCTCGCATAT
    CAGTACCCAG TGCAGTTTGC CAGGCCTCTG GAGGTGGCTA TTGAAGCCAG TAACGGAGCC
    TCTGATTATG GAAACAAGTT TGGGGAGCCT GTTTTGGCCG GATTTGCCCG TTCCTTTGGC
    CTGCGGTTGC CTTCGGGAGA AAGGAGGGAG TGGGTGAAAC CCATTATGTT CAGTGGTGGG
    ATCGGATCCA TGGAGGATGT TCACCGTACT AAGGAGGCCC CAGAGCCTGG CATGCACGTG
    GTTAAAGTGG GAGGTCCTGT CTATAGGATT GGGGTTGGCG GTGGAGCTGC CTCTTCTATT
    CAGGTCCAGG GGGATAACGC CAGCGAGCTG GATTTTGGGG CAGTGCAACG TGGAGATGCA
    GAGATGGAGC AGAAGATGAA TCGGGCCGTA CGGGCCTGCG TGGAGCGAGG AGGCAAGAAC
    CCCATCTGCA GCATTCACGA TCAGGGAGCC GGGGGCAACG GGAACGTCCT GAAGGAGCTC
    AGTGAGCCCC AAGGAGCAGT CATTTACACT AAAAGCTTCC AGCTGGGGGA CCCCACCCTG
    AGTGTTCTGG AGATATGGGG AGCAGAATAT CAGGAGTCCA ACGCTTTGCT CCTGCGCCCC
    GACGATGCCG AATTCCTGCG CTCTGTGTGC CGGCGGGAGA GGTCTCCTGT GGATTTTGTG
    GGCAAGATCA CCGGGGATGG CAGGATTGTG TTGATAAATG GCAGTGACAC AGATCCCGCC
    CCTGACTCCA CTGACCGCAA TGCTGTTCCC GTTGACCTCC AGCTGGAATG GGTCTTAGGA
    AAAATGCCTC GCAAGGAGTT TGTTCTAAAC CGCGTGGCAC CAAACTTGCA GCCGCTGGTG
    CTCCCTAGTG GCCTGACAGT CGGGCAGGCG CTTGATCGCG TGCTGAGGTT GCCGTCCGTA
    GCGAGCAAAC GCTACCTGAC CAACAAGGTG GACCGCTCCG TCACAGGCCT GGTGGCCCAG
    CAGCAGTGCG TGGGGCCTCT TCACACTCCA TTGGCCGACG TGGCTGTGGT GTCTCTGTCT
    TATTCGGACA TTGTAGGAGG AGCTACAGCA ATAGGAGAAC AGCCAATCAA AAGCCTACTG
    AATCCTGCAG CTGGGGCAAG ACTGGCGGTG GCCGAGGCAC TAACCAATCT CCTCTTTGCT
    CAAGTGACGG ACCTAAAGGA TGTGAAATGC AGCGGGAACT GGATGTGGGC CGCCAAGCTG
    CCCGGAGAAG GCGCCTTGCT TTACGACGCC TGTGCAGCTA TGTGTGATGT CATGGCGCAG
    CTCGGCGTTG CCATCGATGG AGGAAAAGAT TCTCTCAGCA TGGCCGCCAG AGTGGGCACA
    GAAACAGTTA AAGCTCCAGG TTCGCTAGTG ATCTCGGTGT ATGCAGTCTG CCCCGACATA
    ACTACCACCG TGACCCCCGA TCTTAAGAAC CCAGGGCAGA AAGGTGTTTT ACTGTATTTG
    CCCCTCTGCC CTGGTCAGCA CCGGCTTGGG GGCAGCGCCC TGGCTCAGTG CTACTCACAG
    CTGGGAGAGA CCCCCCCAGA CCTCGATGAC CCTCAGACTT TAATCTCCTG CTTCAACGTC
    ACCCAACAGC TGCTGAGAGA CCGCGTCCTT AGCGCCGGCC ATGACGTCAG TGATGGAGGC
    CTAGTCACGT GTATCCTGGA AATGGCTTTT GCTGGAAACT GTGGCCTGGA TATTGAGATT
    TCCTCCTCCT GCACCAATGT GCTGGAACTG CTGTTTGCCG AGGAACCGGG CCTGGTTCTA
    GAGGTTGCAG AACAAAGTGT AGAACTGGTC ATGGAACGTT ACAGAGCAGC AGGAGTGGAA
    TGCGTGAGGA TCGGATGCAC CAAGGAACGT GGCCCAGCGT CTATGGTCCG AATCCGTGCC
    AATGGGCAGG AGGTGGTCAA CGAGAAGTTG GGTTCCCTCA GAGCCGTATG GGAGGAGACC
    AGTTTCCAAC TTGAACGCCT TCAGGCCAAT CCAAGCTGTG TATCGCAGGA GGAGGCTGGG
    CTGAGAGTGA GGGAGGGGCC AAGTTATCAC CTGACTTTTA ACCCGTCTGA AATACCTTTG
    GTTCCTCCAT CAGTCGGTGG CAACCAACCG CGTGTTGCTG TTGTGCGTGA AGAGGGTAGC
    AACGGAGACC GGGAAATGGC GGCATCTCTC CTAATGGCTG GATTTCAGGT GTGGGATGTC
    ACCATGGAAG ATCTTCTTGC TGGAGGCACC ACCCTGGACT CTTTCAGGGG ACTTGTATTT
    GTTGGGGGAT TTAGCTACGC TGATGTGTTA GGGTCTGCCA AAGGTTGGGC CGCCTCGGTG
    AAGTTCAATG CCAGCGTGCG CGAGCAGTTC GAGAACTTCC GCCGTAGGGC AGATACTTTC
    AGCCTTGGGG TTTGCAATGG GTGCCAGCTG ATGGCTCTTC TGGGCTGGGT AGGACCCGAT
    AACCCAACAG ACACAGTAGG TGATCTCCCC ACCCAAGGGG TTCTGCTCAG CCACAATCTC
    TCCGGCCGGT TTGAATCTCG ATTTGTCACC CTGAAGATCC AACAGAGTCC TTCCATTTTG
    CTACGAGGGA TGGCCGGGTC CACGCTCGGA GTGTGGGTGG CCCATGGCGA AGGATACATG
    AGGTTCCGAT CTCCGAAAGT GCAGGACTAT GTGACGTCCA ATCACTTGGC GCCGCTCTGT
    TACGTGGATG ATGGAGGCAA GCCGACGGAA GAATACCCAA TGAATCCAAA TGGCTCTCCG
    TTGGGAATTG CTGGTCTTTG CTCTGCAGAT GGGCGGCACT TGGCCATGAT GCCCCACCCA
    GAGCGCTGTG TCATGAAGTG GCAGTGGCCT TGGATGCCGG AAAGCTGGCG CCACTCATTG
    GATGTCTCGC CGTGGATGCG TCTCTTCCAG AATGGGTACC GCTGGTGCCA GGAGAACCCA
    AGCAGGGATA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32769
    Clone ID Related Accession (Same CDS sequence) NM_001142220.3 , XM_012970190.2 , XM_012970191.2
    Accession Version XM_012970191.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3975bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product phosphoribosylformylglycinamidine synthase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970191.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGTGGTCC TGCACTTCTA TCGCTCCTTG CAGAATTCAT CAGAGACCCT GCGGCTGCCC 
    GGTGCGGAGA ATATACAGAG CCTGCAGAGG GAGCTGTGCT ACAATGTCAA TTGGACAGGT
    CCCGCGGCCC CATCCAGCCA GGAGACTGAC GCCCTTCGAT GGCTGTTTAG TTGCCCCTTT
    GACCCTCAGA GTATCTCTGA CACCTCATCC CTTCACTCGG AGCCTTCTGA CCTGCTGGTA
    GAGATCGGCC CAAGACTGAA TTTCTCCACC GCGACTTCCA CCAATGCTGT GTCCATCTGC
    CGCTCTGTTG GCCTGACCAA CGTGGACAGG ATCGAGTGCT CCAAGAGGTA TCTCATCAAG
    TTCCAGACTA GGCCCCAGGG CAGTGAGGAG CAAGAGCTGC TAGCCTCTCT GTACGACCGT
    ATGACGGAAT GCGTTTACCC GGAGCCGGTG AGGAGTTTCG AGGTGTCCGT TAGACCGGAG
    AAAGTGTACG AGGTGGATAT TTTGGGGCAA GGCAGGGCAG CACTGGAAAA GGCCAACACT
    GAGCTAGGTT TGGCCTTCGA CTCCTGGGAT CTTGATTACT ATAGCAGCCT TTTCCAGCGC
    GTGGGTCGGA ACCCCAGCAG CGTGGAGTGC TTTGATCTTG CACAGTCCAA CAGCGAGCAC
    AGCCGCCACT GGTTCTTTAA GGGCAAATTA AAGGTCGACG GGCAGGAGAA GGAGATGTCT
    CTGTTCGACA TGATCATGAA GACTCAGGAC ACCAGTAATC CCAACAACGT CATCAAGTTC
    TGTGACAACA GCAGTGCGAT CCAGGGAAGA GAAGTGGCAT CTCTAATCCC AACAGACCCT
    TCATGTGCTG GGCCCTATCA TCTCTGTCGC TCCACACGGC ACCTGATTTT CACTGCAGAG
    ACCCACAACT TCCCTACTGG CGTGGCCCCA TTCAGCGGCG CTACGACAGG AACAGGAGGG
    CGCATTCGGG ACGTGCAGAG CACCGGCAGG GGAGCACATG TCATTGCTGG GACGGCTGGG
    TACTGCTTTG GGAACCTGCA CATCCCAGGA TACTCTCTTC CCTGGGAGGA CCTCGCATAT
    CAGTACCCAG TGCAGTTTGC CAGGCCTCTG GAGGTGGCTA TTGAAGCCAG TAACGGAGCC
    TCTGATTATG GAAACAAGTT TGGGGAGCCT GTTTTGGCCG GATTTGCCCG TTCCTTTGGC
    CTGCGGTTGC CTTCGGGAGA AAGGAGGGAG TGGGTGAAAC CCATTATGTT CAGTGGTGGG
    ATCGGATCCA TGGAGGATGT TCACCGTACT AAGGAGGCCC CAGAGCCTGG CATGCACGTG
    GTTAAAGTGG GAGGTCCTGT CTATAGGATT GGGGTTGGCG GTGGAGCTGC CTCTTCTATT
    CAGGTCCAGG GGGATAACGC CAGCGAGCTG GATTTTGGGG CAGTGCAACG TGGAGATGCA
    GAGATGGAGC AGAAGATGAA TCGGGCCGTA CGGGCCTGCG TGGAGCGAGG AGGCAAGAAC
    CCCATCTGCA GCATTCACGA TCAGGGAGCC GGGGGCAACG GGAACGTCCT GAAGGAGCTC
    AGTGAGCCCC AAGGAGCAGT CATTTACACT AAAAGCTTCC AGCTGGGGGA CCCCACCCTG
    AGTGTTCTGG AGATATGGGG AGCAGAATAT CAGGAGTCCA ACGCTTTGCT CCTGCGCCCC
    GACGATGCCG AATTCCTGCG CTCTGTGTGC CGGCGGGAGA GGTCTCCTGT GGATTTTGTG
    GGCAAGATCA CCGGGGATGG CAGGATTGTG TTGATAAATG GCAGTGACAC AGATCCCGCC
    CCTGACTCCA CTGACCGCAA TGCTGTTCCC GTTGACCTCC AGCTGGAATG GGTCTTAGGA
    AAAATGCCTC GCAAGGAGTT TGTTCTAAAC CGCGTGGCAC CAAACTTGCA GCCGCTGGTG
    CTCCCTAGTG GCCTGACAGT CGGGCAGGCG CTTGATCGCG TGCTGAGGTT GCCGTCCGTA
    GCGAGCAAAC GCTACCTGAC CAACAAGGTG GACCGCTCCG TCACAGGCCT GGTGGCCCAG
    CAGCAGTGCG TGGGGCCTCT TCACACTCCA TTGGCCGACG TGGCTGTGGT GTCTCTGTCT
    TATTCGGACA TTGTAGGAGG AGCTACAGCA ATAGGAGAAC AGCCAATCAA AAGCCTACTG
    AATCCTGCAG CTGGGGCAAG ACTGGCGGTG GCCGAGGCAC TAACCAATCT CCTCTTTGCT
    CAAGTGACGG ACCTAAAGGA TGTGAAATGC AGCGGGAACT GGATGTGGGC CGCCAAGCTG
    CCCGGAGAAG GCGCCTTGCT TTACGACGCC TGTGCAGCTA TGTGTGATGT CATGGCGCAG
    CTCGGCGTTG CCATCGATGG AGGAAAAGAT TCTCTCAGCA TGGCCGCCAG AGTGGGCACA
    GAAACAGTTA AAGCTCCAGG TTCGCTAGTG ATCTCGGTGT ATGCAGTCTG CCCCGACATA
    ACTACCACCG TGACCCCCGA TCTTAAGAAC CCAGGGCAGA AAGGTGTTTT ACTGTATTTG
    CCCCTCTGCC CTGGTCAGCA CCGGCTTGGG GGCAGCGCCC TGGCTCAGTG CTACTCACAG
    CTGGGAGAGA CCCCCCCAGA CCTCGATGAC CCTCAGACTT TAATCTCCTG CTTCAACGTC
    ACCCAACAGC TGCTGAGAGA CCGCGTCCTT AGCGCCGGCC ATGACGTCAG TGATGGAGGC
    CTAGTCACGT GTATCCTGGA AATGGCTTTT GCTGGAAACT GTGGCCTGGA TATTGAGATT
    TCCTCCTCCT GCACCAATGT GCTGGAACTG CTGTTTGCCG AGGAACCGGG CCTGGTTCTA
    GAGGTTGCAG AACAAAGTGT AGAACTGGTC ATGGAACGTT ACAGAGCAGC AGGAGTGGAA
    TGCGTGAGGA TCGGATGCAC CAAGGAACGT GGCCCAGCGT CTATGGTCCG AATCCGTGCC
    AATGGGCAGG AGGTGGTCAA CGAGAAGTTG GGTTCCCTCA GAGCCGTATG GGAGGAGACC
    AGTTTCCAAC TTGAACGCCT TCAGGCCAAT CCAAGCTGTG TATCGCAGGA GGAGGCTGGG
    CTGAGAGTGA GGGAGGGGCC AAGTTATCAC CTGACTTTTA ACCCGTCTGA AATACCTTTG
    GTTCCTCCAT CAGTCGGTGG CAACCAACCG CGTGTTGCTG TTGTGCGTGA AGAGGGTAGC
    AACGGAGACC GGGAAATGGC GGCATCTCTC CTAATGGCTG GATTTCAGGT GTGGGATGTC
    ACCATGGAAG ATCTTCTTGC TGGAGGCACC ACCCTGGACT CTTTCAGGGG ACTTGTATTT
    GTTGGGGGAT TTAGCTACGC TGATGTGTTA GGGTCTGCCA AAGGTTGGGC CGCCTCGGTG
    AAGTTCAATG CCAGCGTGCG CGAGCAGTTC GAGAACTTCC GCCGTAGGGC AGATACTTTC
    AGCCTTGGGG TTTGCAATGG GTGCCAGCTG ATGGCTCTTC TGGGCTGGGT AGGACCCGAT
    AACCCAACAG ACACAGTAGG TGATCTCCCC ACCCAAGGGG TTCTGCTCAG CCACAATCTC
    TCCGGCCGGT TTGAATCTCG ATTTGTCACC CTGAAGATCC AACAGAGTCC TTCCATTTTG
    CTACGAGGGA TGGCCGGGTC CACGCTCGGA GTGTGGGTGG CCCATGGCGA AGGATACATG
    AGGTTCCGAT CTCCGAAAGT GCAGGACTAT GTGACGTCCA ATCACTTGGC GCCGCTCTGT
    TACGTGGATG ATGGAGGCAA GCCGACGGAA GAATACCCAA TGAATCCAAA TGGCTCTCCG
    TTGGGAATTG CTGGTCTTTG CTCTGCAGAT GGGCGGCACT TGGCCATGAT GCCCCACCCA
    GAGCGCTGTG TCATGAAGTG GCAGTGGCCT TGGATGCCGG AAAGCTGGCG CCACTCATTG
    GATGTCTCGC CGTGGATGCG TCTCTTCCAG AATGGGTACC GCTGGTGCCA GGAGAACCCA
    AGCAGGGATA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012825645.1
    CDS43..4017
    Translation

    Target ORF information:

    RefSeq Version XM_012970191.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phosphoribosylformylglycinamidine synthase (pfas), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970191.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGTGGTCC TGCACTTCTA TCGCTCCTTG CAGAATTCAT CAGAGACCCT GCGGCTGCCC 
    GGTGCGGAGA ATATACAGAG CCTGCAGAGG GAGCTGTGCT ACAATGTCAA TTGGACAGGT
    CCCGCGGCCC CATCCAGCCA GGAGACTGAC GCCCTTCGAT GGCTGTTTAG TTGCCCCTTT
    GACCCTCAGA GTATCTCTGA CACCTCATCC CTTCACTCGG AGCCTTCTGA CCTGCTGGTA
    GAGATCGGCC CAAGACTGAA TTTCTCCACC GCGACTTCCA CCAATGCTGT GTCCATCTGC
    CGCTCTGTTG GCCTGACCAA CGTGGACAGG ATCGAGTGCT CCAAGAGGTA TCTCATCAAG
    TTCCAGACTA GGCCCCAGGG CAGTGAGGAG CAAGAGCTGC TAGCCTCTCT GTACGACCGT
    ATGACGGAAT GCGTTTACCC GGAGCCGGTG AGGAGTTTCG AGGTGTCCGT TAGACCGGAG
    AAAGTGTACG AGGTGGATAT TTTGGGGCAA GGCAGGGCAG CACTGGAAAA GGCCAACACT
    GAGCTAGGTT TGGCCTTCGA CTCCTGGGAT CTTGATTACT ATAGCAGCCT TTTCCAGCGC
    GTGGGTCGGA ACCCCAGCAG CGTGGAGTGC TTTGATCTTG CACAGTCCAA CAGCGAGCAC
    AGCCGCCACT GGTTCTTTAA GGGCAAATTA AAGGTCGACG GGCAGGAGAA GGAGATGTCT
    CTGTTCGACA TGATCATGAA GACTCAGGAC ACCAGTAATC CCAACAACGT CATCAAGTTC
    TGTGACAACA GCAGTGCGAT CCAGGGAAGA GAAGTGGCAT CTCTAATCCC AACAGACCCT
    TCATGTGCTG GGCCCTATCA TCTCTGTCGC TCCACACGGC ACCTGATTTT CACTGCAGAG
    ACCCACAACT TCCCTACTGG CGTGGCCCCA TTCAGCGGCG CTACGACAGG AACAGGAGGG
    CGCATTCGGG ACGTGCAGAG CACCGGCAGG GGAGCACATG TCATTGCTGG GACGGCTGGG
    TACTGCTTTG GGAACCTGCA CATCCCAGGA TACTCTCTTC CCTGGGAGGA CCTCGCATAT
    CAGTACCCAG TGCAGTTTGC CAGGCCTCTG GAGGTGGCTA TTGAAGCCAG TAACGGAGCC
    TCTGATTATG GAAACAAGTT TGGGGAGCCT GTTTTGGCCG GATTTGCCCG TTCCTTTGGC
    CTGCGGTTGC CTTCGGGAGA AAGGAGGGAG TGGGTGAAAC CCATTATGTT CAGTGGTGGG
    ATCGGATCCA TGGAGGATGT TCACCGTACT AAGGAGGCCC CAGAGCCTGG CATGCACGTG
    GTTAAAGTGG GAGGTCCTGT CTATAGGATT GGGGTTGGCG GTGGAGCTGC CTCTTCTATT
    CAGGTCCAGG GGGATAACGC CAGCGAGCTG GATTTTGGGG CAGTGCAACG TGGAGATGCA
    GAGATGGAGC AGAAGATGAA TCGGGCCGTA CGGGCCTGCG TGGAGCGAGG AGGCAAGAAC
    CCCATCTGCA GCATTCACGA TCAGGGAGCC GGGGGCAACG GGAACGTCCT GAAGGAGCTC
    AGTGAGCCCC AAGGAGCAGT CATTTACACT AAAAGCTTCC AGCTGGGGGA CCCCACCCTG
    AGTGTTCTGG AGATATGGGG AGCAGAATAT CAGGAGTCCA ACGCTTTGCT CCTGCGCCCC
    GACGATGCCG AATTCCTGCG CTCTGTGTGC CGGCGGGAGA GGTCTCCTGT GGATTTTGTG
    GGCAAGATCA CCGGGGATGG CAGGATTGTG TTGATAAATG GCAGTGACAC AGATCCCGCC
    CCTGACTCCA CTGACCGCAA TGCTGTTCCC GTTGACCTCC AGCTGGAATG GGTCTTAGGA
    AAAATGCCTC GCAAGGAGTT TGTTCTAAAC CGCGTGGCAC CAAACTTGCA GCCGCTGGTG
    CTCCCTAGTG GCCTGACAGT CGGGCAGGCG CTTGATCGCG TGCTGAGGTT GCCGTCCGTA
    GCGAGCAAAC GCTACCTGAC CAACAAGGTG GACCGCTCCG TCACAGGCCT GGTGGCCCAG
    CAGCAGTGCG TGGGGCCTCT TCACACTCCA TTGGCCGACG TGGCTGTGGT GTCTCTGTCT
    TATTCGGACA TTGTAGGAGG AGCTACAGCA ATAGGAGAAC AGCCAATCAA AAGCCTACTG
    AATCCTGCAG CTGGGGCAAG ACTGGCGGTG GCCGAGGCAC TAACCAATCT CCTCTTTGCT
    CAAGTGACGG ACCTAAAGGA TGTGAAATGC AGCGGGAACT GGATGTGGGC CGCCAAGCTG
    CCCGGAGAAG GCGCCTTGCT TTACGACGCC TGTGCAGCTA TGTGTGATGT CATGGCGCAG
    CTCGGCGTTG CCATCGATGG AGGAAAAGAT TCTCTCAGCA TGGCCGCCAG AGTGGGCACA
    GAAACAGTTA AAGCTCCAGG TTCGCTAGTG ATCTCGGTGT ATGCAGTCTG CCCCGACATA
    ACTACCACCG TGACCCCCGA TCTTAAGAAC CCAGGGCAGA AAGGTGTTTT ACTGTATTTG
    CCCCTCTGCC CTGGTCAGCA CCGGCTTGGG GGCAGCGCCC TGGCTCAGTG CTACTCACAG
    CTGGGAGAGA CCCCCCCAGA CCTCGATGAC CCTCAGACTT TAATCTCCTG CTTCAACGTC
    ACCCAACAGC TGCTGAGAGA CCGCGTCCTT AGCGCCGGCC ATGACGTCAG TGATGGAGGC
    CTAGTCACGT GTATCCTGGA AATGGCTTTT GCTGGAAACT GTGGCCTGGA TATTGAGATT
    TCCTCCTCCT GCACCAATGT GCTGGAACTG CTGTTTGCCG AGGAACCGGG CCTGGTTCTA
    GAGGTTGCAG AACAAAGTGT AGAACTGGTC ATGGAACGTT ACAGAGCAGC AGGAGTGGAA
    TGCGTGAGGA TCGGATGCAC CAAGGAACGT GGCCCAGCGT CTATGGTCCG AATCCGTGCC
    AATGGGCAGG AGGTGGTCAA CGAGAAGTTG GGTTCCCTCA GAGCCGTATG GGAGGAGACC
    AGTTTCCAAC TTGAACGCCT TCAGGCCAAT CCAAGCTGTG TATCGCAGGA GGAGGCTGGG
    CTGAGAGTGA GGGAGGGGCC AAGTTATCAC CTGACTTTTA ACCCGTCTGA AATACCTTTG
    GTTCCTCCAT CAGTCGGTGG CAACCAACCG CGTGTTGCTG TTGTGCGTGA AGAGGGTAGC
    AACGGAGACC GGGAAATGGC GGCATCTCTC CTAATGGCTG GATTTCAGGT GTGGGATGTC
    ACCATGGAAG ATCTTCTTGC TGGAGGCACC ACCCTGGACT CTTTCAGGGG ACTTGTATTT
    GTTGGGGGAT TTAGCTACGC TGATGTGTTA GGGTCTGCCA AAGGTTGGGC CGCCTCGGTG
    AAGTTCAATG CCAGCGTGCG CGAGCAGTTC GAGAACTTCC GCCGTAGGGC AGATACTTTC
    AGCCTTGGGG TTTGCAATGG GTGCCAGCTG ATGGCTCTTC TGGGCTGGGT AGGACCCGAT
    AACCCAACAG ACACAGTAGG TGATCTCCCC ACCCAAGGGG TTCTGCTCAG CCACAATCTC
    TCCGGCCGGT TTGAATCTCG ATTTGTCACC CTGAAGATCC AACAGAGTCC TTCCATTTTG
    CTACGAGGGA TGGCCGGGTC CACGCTCGGA GTGTGGGTGG CCCATGGCGA AGGATACATG
    AGGTTCCGAT CTCCGAAAGT GCAGGACTAT GTGACGTCCA ATCACTTGGC GCCGCTCTGT
    TACGTGGATG ATGGAGGCAA GCCGACGGAA GAATACCCAA TGAATCCAAA TGGCTCTCCG
    TTGGGAATTG CTGGTCTTTG CTCTGCAGAT GGGCGGCACT TGGCCATGAT GCCCCACCCA
    GAGCGCTGTG TCATGAAGTG GCAGTGGCCT TGGATGCCGG AAAGCTGGCG CCACTCATTG
    GATGTCTCGC CGTGGATGCG TCTCTTCCAG AATGGGTACC GCTGGTGCCA GGAGAACCCA
    AGCAGGGATA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32770
    Clone ID Related Accession (Same CDS sequence) XM_012970192.2
    Accession Version XM_012970192.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3972bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product phosphoribosylformylglycinamidine synthase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970192.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGTGGTCC TGCACTTCTA TCGCTCCTTG CAGAATTCAT CAGAGACCCT GCGGCTGCCC 
    GGTGCGGAGA ATATACAGAG CCTGCAGAGG GAGCTGTGCT ACAATGTCAA TTGGACAGGT
    CCCGCGGCCC CATCCAGCCA GGAGACTGAC GCCCTTCGAT GGCTGTTTAG TTGCCCCTTT
    GACCCTCAGA GTATCTCTGA CACCTCATCC CTTCACTCGG AGCCTTCTGA CCTGCTGGTA
    GAGATCGGCC CAAGACTGAA TTTCTCCACC GCGACTTCCA CCAATGCTGT GTCCATCTGC
    CGCTCTGTTG GCCTGACCAA CGTGGACAGG ATCGAGTGCT CCAAGAGGTA TCTCATCAAG
    TTCCAGACTA GGCCCCAGGG CAGTGAGGAG CAAGAGCTGC TAGCCTCTCT GTACGACCGT
    ATGACGGAAT GCGTTTACCC GGAGCCGGTG AGGAGTTTCG AGGTGTCCGT TAGACCGGAG
    AAAGTGTACG AGGTGGATAT TTTGGGGCAA GGCAGGGCAG CACTGGAAAA GGCCAACACT
    GAGCTAGGTT TGGCCTTCGA CTCCTGGGAT CTTGATTACT ATAGCAGCCT TTTCCAGCGC
    GTGGGTCGGA ACCCCAGCAG CGTGGAGTGC TTTGATCTTG CACAGTCCAA CAGCGAGCAC
    AGCCGCCACT GGTTCTTTAA GGGCAAATTA AAGGTCGACG GGCAGGAGAA GGAGATGTCT
    CTGTTCGACA TGATCATGAA GACTCAGGAC ACCAGTAATC CCAACAACGT CATCAAGTTC
    TGTGACAACA GCAGTGCGAT CCAGGGAAGA GAAGTGGCAT CTCTAATCCC AACAGACCCT
    TCATGTGCTG GGCCCTATCA TCTCTGTCGC TCCACACGGC ACCTGATTTT CACTGCAGAG
    ACCCACAACT TCCCTACTGG CGTGGCCCCA TTCAGCGGCG CTACGACAGG AACAGGAGGG
    CGCATTCGGG ACGTGCAGAG CACCGGCAGG GGAGCACATG TCATTGCTGG GACGGCTGGG
    TACTGCTTTG GGAACCTGCA CATCCCAGGA TACTCTCTTC CCTGGGAGGA CCTCGCATAT
    CAGTACCCAG TGCAGTTTGC CAGGCCTCTG GAGGTGGCTA TTGAAGCCAG TAACGGAGCC
    TCTGATTATG GAAACAAGTT TGGGGAGCCT GTTTTGGCCG GATTTGCCCG TTCCTTTGGC
    CTGCGGTTGC CTTCGGGAGA AAGGAGGGAG TGGGTGAAAC CCATTATGTT CAGTGGTGGG
    ATCGGATCCA TGGAGGATGT TCACCGTACT AAGGAGGCCC CAGAGCCTGG CATGCACGTG
    GTTAAAGTGG GAGGTCCTGT CTATAGGATT GGGGTTGGCG GTGGAGCTGC CTCTTCTATT
    CAGGTCCAGG GGGATAACGC CAGCGAGCTG GATTTTGGGG CAGTGCAACG TGGAGATGCA
    GAGATGGAGC AGAAGATGAA TCGGGCCGTA CGGGCCTGCG TGGAGCGAGG AGGCAAGAAC
    CCCATCTGCA GCATTCACGA TCAGGGAGCC GGGGGCAACG GGAACGTCCT GAAGGAGCTC
    AGTGAGCCCC AAGGAGCAGT CATTTACACT AAAAGCTTCC AGCTGGGGGA CCCCACCCTG
    AGTGTTCTGG AGATATGGGG AGCAGAATAT CAGGAGTCCA ACGCTTTGCT CCTGCGCCCC
    GACGATGCCG AATTCCTGCG CTCTGTGTGC CGGCGGGAGA GGTCTCCTGT GGATTTTGTG
    GGCAAGATCA CCGGGGATGG CAGGATTGTG TTGATAAATG GCAGTGACAC AGATCCCGCC
    CCTGACTCCA CTGACCGCAA TGCTGTTCCC GTTGACCTCC AGCTGGAATG GGTCTTAGGA
    AAAATGCCTC GCAAGGAGTT TGTTCTAAAC CGCGTGGCAC CAAACTTGCA GCCGCTGGTG
    CTCCCTAGTG GCCTGACAGT CGGGCAGGCG CTTGATCGCG TGCTGAGGTT GCCGTCCGTA
    GCGAGCAAAC GCTACCTGAC CAACAAGGTG GACCGCTCCG TCACAGGCCT GGTGGCCCAG
    CAGCAGTGCG TGGGGCCTCT TCACACTCCA TTGGCCGACG TGGCTGTGGT GTCTCTGTCT
    TATTCGGACA TTGTAGGAGG AGCTACAGCA ATAGGAGAAC AGCCAATCAA AAGCCTACTG
    AATCCTGCAG CTGGGGCAAG ACTGGCGGTG GCCGAGGCAC TAACCAATCT CCTCTTTGCT
    CAAGTGACGG ACCTAAAGGA TGTGAAATGC AGCGGGAACT GGATGTGGGC CGCCAAGCTG
    CCCGGAGAAG GCGCCTTGCT TTACGACGCC TGTGCAGCTA TGTGTGATGT CATGGCGCAG
    CTCGGCGTTG CCATCGATGG AGGAAAAGAT TCTCTCAGCA TGGCCGCCAG AGTGGGCACA
    GAAACAGTTA AAGCTCCAGG TTCGCTAGTG ATCTCGGTGT ATGCAGTCTG CCCCGACATA
    ACTACCACCG TGACCCCCGA TCTTAAGAAC CCAGGGCAGA AAGGTGTTTT ACTGTATTTG
    CCCCTCTGCC CTGGTCAGCA CCGGCTTGGG GGCAGCGCCC TGGCTCAGTG CTACTCACAG
    CTGGGAGAGA CCCCCCCAGA CCTCGATGAC CCTCAGACTT TAATCTCCTG CTTCAACGTC
    ACCCAACAGC TGCTGAGAGA CCGCGTCCTT AGCGCCGGCC ATGACGTCAG TGATGGAGGC
    CTAGTCACGT GTATCCTGGA AATGGCTTTT GCTGGAAACT GTGGCCTGGA TATTGAGATT
    TCCTCCTCCT GCACCAATGT GCTGGAACTG CTGTTTGCCG AGGAACCGGG CCTGGTTCTA
    GAGGTTGCAG AACAAAGTGT AGAACTGGTC ATGGAACGTT ACAGAGCAGC AGGAGTGGAA
    TGCGTGAGGA TCGGATGCAC CAAGGAACGT GGCCCAGCGT CTATGGTCCG AATCCGTGCC
    AATGGGCAGG AGGTGGTCAA CGAGAAGTTG GGTTCCCTCA GAGCCGTATG GGAGGAGACC
    AGTTTCCAAC TTGAACGCCT TCAGGCCAAT CCAAGCTGTG TATCGCAGGA GGAGGCTGGG
    CTGAGAGTGA GGGAGGGGCC AAGTTATCAC CTGACTTTTA ACCCGTCTGA AATACCTTTG
    GTTCCTCCAT CAGTCGGTGG CAACCAACCG CGTGTTGCTG TTGTGCGTGA AGAGGGTAGC
    AACGGAGACC GGGAAATGGC GGCATCTCTC CTAATGGCTG GATTTCAGGT GTGGGATGTC
    ACCATGGAAG ATCTTCTTGC TGGAGGCACC ACCCTGGACT CTTTCAGGGG ACTTGTATTT
    GTTGGGGGAT TTAGCTACGC TGATGTGTTA GGGTCTGCCA AAGGTTGGGC CGCCTCGGTG
    AAGTTCAATG CCAGCGTGCG CGAGCAGTTC GAGAACTTCC GCCGTAGGGC AGATACTTTC
    AGCCTTGGGG TTTGCAATGG GTGCCAGCTG ATGGCTCTTC TGGGCTGGGT AGGACCCGAT
    AACCCAACAG ACACAGGTGA TCTCCCCACC CAAGGGGTTC TGCTCAGCCA CAATCTCTCC
    GGCCGGTTTG AATCTCGATT TGTCACCCTG AAGATCCAAC AGAGTCCTTC CATTTTGCTA
    CGAGGGATGG CCGGGTCCAC GCTCGGAGTG TGGGTGGCCC ATGGCGAAGG ATACATGAGG
    TTCCGATCTC CGAAAGTGCA GGACTATGTG ACGTCCAATC ACTTGGCGCC GCTCTGTTAC
    GTGGATGATG GAGGCAAGCC GACGGAAGAA TACCCAATGA ATCCAAATGG CTCTCCGTTG
    GGAATTGCTG GTCTTTGCTC TGCAGATGGG CGGCACTTGG CCATGATGCC CCACCCAGAG
    CGCTGTGTCA TGAAGTGGCA GTGGCCTTGG ATGCCGGAAA GCTGGCGCCA CTCATTGGAT
    GTCTCGCCGT GGATGCGTCT CTTCCAGAAT GGGTACCGCT GGTGCCAGGA GAACCCAAGC
    AGGGATAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012825646.1
    CDS129..4100
    Translation

    Target ORF information:

    RefSeq Version XM_012970192.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phosphoribosylformylglycinamidine synthase (pfas), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970192.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGTGGTCC TGCACTTCTA TCGCTCCTTG CAGAATTCAT CAGAGACCCT GCGGCTGCCC 
    GGTGCGGAGA ATATACAGAG CCTGCAGAGG GAGCTGTGCT ACAATGTCAA TTGGACAGGT
    CCCGCGGCCC CATCCAGCCA GGAGACTGAC GCCCTTCGAT GGCTGTTTAG TTGCCCCTTT
    GACCCTCAGA GTATCTCTGA CACCTCATCC CTTCACTCGG AGCCTTCTGA CCTGCTGGTA
    GAGATCGGCC CAAGACTGAA TTTCTCCACC GCGACTTCCA CCAATGCTGT GTCCATCTGC
    CGCTCTGTTG GCCTGACCAA CGTGGACAGG ATCGAGTGCT CCAAGAGGTA TCTCATCAAG
    TTCCAGACTA GGCCCCAGGG CAGTGAGGAG CAAGAGCTGC TAGCCTCTCT GTACGACCGT
    ATGACGGAAT GCGTTTACCC GGAGCCGGTG AGGAGTTTCG AGGTGTCCGT TAGACCGGAG
    AAAGTGTACG AGGTGGATAT TTTGGGGCAA GGCAGGGCAG CACTGGAAAA GGCCAACACT
    GAGCTAGGTT TGGCCTTCGA CTCCTGGGAT CTTGATTACT ATAGCAGCCT TTTCCAGCGC
    GTGGGTCGGA ACCCCAGCAG CGTGGAGTGC TTTGATCTTG CACAGTCCAA CAGCGAGCAC
    AGCCGCCACT GGTTCTTTAA GGGCAAATTA AAGGTCGACG GGCAGGAGAA GGAGATGTCT
    CTGTTCGACA TGATCATGAA GACTCAGGAC ACCAGTAATC CCAACAACGT CATCAAGTTC
    TGTGACAACA GCAGTGCGAT CCAGGGAAGA GAAGTGGCAT CTCTAATCCC AACAGACCCT
    TCATGTGCTG GGCCCTATCA TCTCTGTCGC TCCACACGGC ACCTGATTTT CACTGCAGAG
    ACCCACAACT TCCCTACTGG CGTGGCCCCA TTCAGCGGCG CTACGACAGG AACAGGAGGG
    CGCATTCGGG ACGTGCAGAG CACCGGCAGG GGAGCACATG TCATTGCTGG GACGGCTGGG
    TACTGCTTTG GGAACCTGCA CATCCCAGGA TACTCTCTTC CCTGGGAGGA CCTCGCATAT
    CAGTACCCAG TGCAGTTTGC CAGGCCTCTG GAGGTGGCTA TTGAAGCCAG TAACGGAGCC
    TCTGATTATG GAAACAAGTT TGGGGAGCCT GTTTTGGCCG GATTTGCCCG TTCCTTTGGC
    CTGCGGTTGC CTTCGGGAGA AAGGAGGGAG TGGGTGAAAC CCATTATGTT CAGTGGTGGG
    ATCGGATCCA TGGAGGATGT TCACCGTACT AAGGAGGCCC CAGAGCCTGG CATGCACGTG
    GTTAAAGTGG GAGGTCCTGT CTATAGGATT GGGGTTGGCG GTGGAGCTGC CTCTTCTATT
    CAGGTCCAGG GGGATAACGC CAGCGAGCTG GATTTTGGGG CAGTGCAACG TGGAGATGCA
    GAGATGGAGC AGAAGATGAA TCGGGCCGTA CGGGCCTGCG TGGAGCGAGG AGGCAAGAAC
    CCCATCTGCA GCATTCACGA TCAGGGAGCC GGGGGCAACG GGAACGTCCT GAAGGAGCTC
    AGTGAGCCCC AAGGAGCAGT CATTTACACT AAAAGCTTCC AGCTGGGGGA CCCCACCCTG
    AGTGTTCTGG AGATATGGGG AGCAGAATAT CAGGAGTCCA ACGCTTTGCT CCTGCGCCCC
    GACGATGCCG AATTCCTGCG CTCTGTGTGC CGGCGGGAGA GGTCTCCTGT GGATTTTGTG
    GGCAAGATCA CCGGGGATGG CAGGATTGTG TTGATAAATG GCAGTGACAC AGATCCCGCC
    CCTGACTCCA CTGACCGCAA TGCTGTTCCC GTTGACCTCC AGCTGGAATG GGTCTTAGGA
    AAAATGCCTC GCAAGGAGTT TGTTCTAAAC CGCGTGGCAC CAAACTTGCA GCCGCTGGTG
    CTCCCTAGTG GCCTGACAGT CGGGCAGGCG CTTGATCGCG TGCTGAGGTT GCCGTCCGTA
    GCGAGCAAAC GCTACCTGAC CAACAAGGTG GACCGCTCCG TCACAGGCCT GGTGGCCCAG
    CAGCAGTGCG TGGGGCCTCT TCACACTCCA TTGGCCGACG TGGCTGTGGT GTCTCTGTCT
    TATTCGGACA TTGTAGGAGG AGCTACAGCA ATAGGAGAAC AGCCAATCAA AAGCCTACTG
    AATCCTGCAG CTGGGGCAAG ACTGGCGGTG GCCGAGGCAC TAACCAATCT CCTCTTTGCT
    CAAGTGACGG ACCTAAAGGA TGTGAAATGC AGCGGGAACT GGATGTGGGC CGCCAAGCTG
    CCCGGAGAAG GCGCCTTGCT TTACGACGCC TGTGCAGCTA TGTGTGATGT CATGGCGCAG
    CTCGGCGTTG CCATCGATGG AGGAAAAGAT TCTCTCAGCA TGGCCGCCAG AGTGGGCACA
    GAAACAGTTA AAGCTCCAGG TTCGCTAGTG ATCTCGGTGT ATGCAGTCTG CCCCGACATA
    ACTACCACCG TGACCCCCGA TCTTAAGAAC CCAGGGCAGA AAGGTGTTTT ACTGTATTTG
    CCCCTCTGCC CTGGTCAGCA CCGGCTTGGG GGCAGCGCCC TGGCTCAGTG CTACTCACAG
    CTGGGAGAGA CCCCCCCAGA CCTCGATGAC CCTCAGACTT TAATCTCCTG CTTCAACGTC
    ACCCAACAGC TGCTGAGAGA CCGCGTCCTT AGCGCCGGCC ATGACGTCAG TGATGGAGGC
    CTAGTCACGT GTATCCTGGA AATGGCTTTT GCTGGAAACT GTGGCCTGGA TATTGAGATT
    TCCTCCTCCT GCACCAATGT GCTGGAACTG CTGTTTGCCG AGGAACCGGG CCTGGTTCTA
    GAGGTTGCAG AACAAAGTGT AGAACTGGTC ATGGAACGTT ACAGAGCAGC AGGAGTGGAA
    TGCGTGAGGA TCGGATGCAC CAAGGAACGT GGCCCAGCGT CTATGGTCCG AATCCGTGCC
    AATGGGCAGG AGGTGGTCAA CGAGAAGTTG GGTTCCCTCA GAGCCGTATG GGAGGAGACC
    AGTTTCCAAC TTGAACGCCT TCAGGCCAAT CCAAGCTGTG TATCGCAGGA GGAGGCTGGG
    CTGAGAGTGA GGGAGGGGCC AAGTTATCAC CTGACTTTTA ACCCGTCTGA AATACCTTTG
    GTTCCTCCAT CAGTCGGTGG CAACCAACCG CGTGTTGCTG TTGTGCGTGA AGAGGGTAGC
    AACGGAGACC GGGAAATGGC GGCATCTCTC CTAATGGCTG GATTTCAGGT GTGGGATGTC
    ACCATGGAAG ATCTTCTTGC TGGAGGCACC ACCCTGGACT CTTTCAGGGG ACTTGTATTT
    GTTGGGGGAT TTAGCTACGC TGATGTGTTA GGGTCTGCCA AAGGTTGGGC CGCCTCGGTG
    AAGTTCAATG CCAGCGTGCG CGAGCAGTTC GAGAACTTCC GCCGTAGGGC AGATACTTTC
    AGCCTTGGGG TTTGCAATGG GTGCCAGCTG ATGGCTCTTC TGGGCTGGGT AGGACCCGAT
    AACCCAACAG ACACAGGTGA TCTCCCCACC CAAGGGGTTC TGCTCAGCCA CAATCTCTCC
    GGCCGGTTTG AATCTCGATT TGTCACCCTG AAGATCCAAC AGAGTCCTTC CATTTTGCTA
    CGAGGGATGG CCGGGTCCAC GCTCGGAGTG TGGGTGGCCC ATGGCGAAGG ATACATGAGG
    TTCCGATCTC CGAAAGTGCA GGACTATGTG ACGTCCAATC ACTTGGCGCC GCTCTGTTAC
    GTGGATGATG GAGGCAAGCC GACGGAAGAA TACCCAATGA ATCCAAATGG CTCTCCGTTG
    GGAATTGCTG GTCTTTGCTC TGCAGATGGG CGGCACTTGG CCATGATGCC CCACCCAGAG
    CGCTGTGTCA TGAAGTGGCA GTGGCCTTGG ATGCCGGAAA GCTGGCGCCA CTCATTGGAT
    GTCTCGCCGT GGATGCGTCT CTTCCAGAAT GGGTACCGCT GGTGCCAGGA GAACCCAAGC
    AGGGATAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.