brf1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	brf1
Entrez Gene ID	100170638
Full Name	BRF1, RNA polymerase III transcription initiation factor 90 kDa subunit
Synonyms	brf,gtf3b,hbrf,taf3b2,taf3c,tafiii90,tf3b90,tfiiib90
General protein information	Preferred Names BRF1, RNA polymerase III transcription initiation factor 90 kDa subunit Names transcription factor IIIB 90 kDa subunit BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001195621.1	NP_001182550.1	transcription factor IIIB 90 kDa subunit

Pathways from BioSystems

Homologies

	BRF1	XP_001098066.2
Bos taurus (cattle)	BRF1	XP_005222395.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004233	XP_313145.5
Caenorhabditis elegans (roundworm)	brf-1	NP_495526.1
Saccharomyces cerevisiae (baker's yeast)	BRF1	NP_011762.1
Kluyveromyces lactis	KLLA0A05434g	XP_451240.1
Eremothecium gossypii	AGOS_AEL213W	NP_984648.1
Schizosaccharomyces pombe (fission yeast)	brf1	NP_596265.2
Xenopus tropicalis (tropical clawed frog)	brf1	NP_001182550.1
Homo sapiens (human)	BRF1	NP_001510.2
Mus musculus (house mouse)	Brf1	NP_082469.2
Gallus gallus (chicken)	BRF1	XP_004936477.1
Drosophila melanogaster (fruit fly)	Brf	NP_650616.2
Canis lupus familiaris (dog)	BRF1	XP_005623949.1
Rattus norvegicus (Norway rat)	Brf1	NP_001100231.1
Danio rerio (zebrafish)	brf1b	NP_956183.1

Gene Ontology

The following brf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the brf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa20287	NM_001195621.1 Latest version!	Xenopus tropicalis BRF1, RNA polymerase III transcription initiation factor 90 kDa subunit (brf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

brf1 ( NM_001195621.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001182550.1 Xenopus tropicalis BRF1, RNA polymerase III transcription initiation factor 90 kDa subunit (brf1), mRNA.

Price & Availability↑

CloneID	OXa20287
Clone ID Related Accession (Same CDS sequence)	NM_001195621.1
Accession Version	NM_001195621.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1998bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-06
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transcription factor IIIB 90 kDa subunit
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAMC03032326.1, AAMC03032325.1 and AAMC03032324.1. On Sep 22, 2010 this sequence version replaced XM_002938794.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1696415, SAMEA1696417 [ECO:0000348] ##Evidence-Data-END##

ATGAGCAGCC GGGTGTGTAA GAACTGCGGT TGTACCGATA TAGACGTAGA TGCGGCCCGC 
GGGGATGCGG TGTGTACGGG CTGCGGCTCG GTGCTGGAGG ATAATATCAT CGTGTCTGAG 
GTGCAGTTCG TGGAGGGCGC AGGCGGGATC TCCTCGGCAG TTGGACAGTT CGTCTCCTCG 
GAATGTTCTG GTAAAACTCC ATCACTGGGA GAAGGATTTT ACACCAGCGT GGGAAAGGAG 
TCCCGTGCAC AAACTCTACA GAATGGGAAG CGTCAGATTC ACAACTTGGG AGTGCAGTTA 
CAGCTAAATA AGCATTGCCT GGACACTGCC TTCAACTTCT TTAAAATGGC TGTCAGCAAG 
CACCTTACCC GTGGTAGGAA GATGACCCAT GTCATAGCCG CCTGCTTGTA CTTGGTCTGT 
AGGACAGAAG GAACTCCCCA TATGTTACTT GATCTCAGTG ACCTTCTTCA GGTGAATGTG 
TATGTCCTTG GCAAGACCTT TCTTCTCTTA GCCAGAGAAC TCTGCATCAA TGCTCCTGCT 
ATAGACCCCT GCTTATATAT TCCTCGATTT GCACACTTGC TGGAGTTTGG CGATAAAAAT 
CATGAAGTAT CAATGACTGC ACTGAGGTTG GTCCAAAGGA TGAAAAGAGA TTGGATGCAC 
ACTGGGCGCC GCCCATCTGG ACTCTGCGGA GCTGCACTGC TTGTTGCATC CAGAATGCAT 
GATTTCCGGC GGACCATTAA AGAGGTCATC AGGGTGGTAA AGGTGTGTGA GTCTACACTG 
AGGAAAAGAT TAACAGAATT TGAAGATACT CCAACAAGTC AACTGACAAT TGATGAATTT 
ATGAAGATTG ATTTGGAACA AGAGTGTGAT CCTCCTTCCT TTACTGCTGG TCAAAGGAAG 
GTTAAAATAC AACAGCTTGA GCAAGAGTTG GCAAAAAAAC TTGATGAAGT AGAAGGGGAA 
ATATCAAGTT ATCAAGATGA AATAGAGAAT GAGCTGGAGA GTAGCAGACC AAAGCCCAAA 
GGAATATATG CTTCATATGC TAAAAATGGT TCAGAAGACA ACACTTCTAG CATACTTGCA 
GAAGAAGATG ATGATGAAGA CCTGGAAGCA GCCGCTAATC ACTTAAACAA GGATTTTTAC 
AGTGAACTTG CTAATGATAC ATCTAAAGGG ATTTGTTATG AGCACACCGT GAAGCCACTT 
CCTATAGAAT CTCTACTAGG CCCTCTCCCC ACTGCTGCAA GCTTGGGGAT TACAGACTCC 
ATCAAGGAAT GCATAGCAAT AAATGAGCAA GAGAAAAGTG ACAATGCAGG AGACGGCGAA 
CTCGACCTTA ATGGAATTGA TGACAATGAA ATAGACAGGT ATATTCTCAA TGAGAAGGAA 
GCAGAAATAA AGAAAGAGAT GTGGATGAAA GAGAATGAGG ACTACCTTCG AGAACAAAAG 
GAAAAGGAAG CGCGTATAGC TAAAGAGAAG GAGCTTGGTA TTTACAAAGA GCAAAAGCCA 
AGGAAACCTT CCAAGCGGAG GGCTCCAATC CAAGCCAGCA CTGCTGGGGA GGCAATTGAA 
AAGATGCTGG AGCAGAAGAA AATCTCCAGT AAAATTAACT ATGATGTACT CCGGGACCTC 
AACAGCAAAG GGAGCAGTAC TCCAAAGCAC GATAAGAACG ATATGGAGGA AGGGATCACT 
AGCAACCAAC TCTCCCGCAG GAAATCTGCT GCCAGTAAAA TTCTGTCCAA TCCCGTCAGC 
AACGCCGGCA AAAGGATAAG ACCACTGGTT TCAACGCAGC CATCTAAGAA AGTAGCATTT 
GAACAGGAAG TCAGAAATGA ACTTGAGTCA ACACCCCCAA AGATAGTAGT GGAGAGTGGG 
CCAGTCACAT ACAATCACGA GGATGATGCC GCCGCCGCCG AAGAAGAAGA GGAGGAAGAG 
GAAGAACACT GTGTGAGCGC ACTTCAGTTA ATGGGGGGAA ATGATTACGG CTGTGATATG 
GATGAGGATG ATTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001182550.1
CDS	139..2136
Translation	MSSRVCKNCGCTDIDVDAARGDAVCTGCGSVLEDNIIVSEVQFVEGAGGISSAVGQFVSSECSGKTPSLGEGFYTSVGKESRAQTLQNGKRQIHNLGVQLQLNKHCLDTAFNFFKMAVSKHLTRGRKMTHVIAACLYLVCRTEGTPHMLLDLSDLLQVNVYVLGKTFLLLARELCINAPAIDPCLYIPRFAHLLEFGDKNHEVSMTALRLVQRMKRDWMHTGRRPSGLCGAALLVASRMHDFRRTIKEVIRVVKVCESTLRKRLTEFEDTPTSQLTIDEFMKIDLEQECDPPSFTAGQRKVKIQQLEQELAKKLDEVEGEISSYQDEIENELESSRPKPKGIYASYAKNGSEDNTSSILAEEDDDEDLEAAANHLNKDFYSELANDTSKGICYEHTVKPLPIESLLGPLPTAASLGITDSIKECIAINEQEKSDNAGDGELDLNGIDDNEIDRYILNEKEAEIKKEMWMKENEDYLREQKEKEARIAKEKELGIYKEQKPRKPSKRRAPIQASTAGEAIEKMLEQKKISSKINYDVLRDLNSKGSSTPKHDKNDMEEGITSNQLSRRKSAASKILSNPVSNAGKRIRPLVSTQPSKKVAFEQEVRNELESTPPKIVVESGPVTYNHEDDAAAAEEEEEEEEEHCVSALQLMGGNDYGCDMDEDDY

Target ORF information:

RefSeq Version	NM_001195621.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis BRF1, RNA polymerase III transcription initiation factor 90 kDa subunit (brf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001195621.1

ORF Insert Sequence:

ATGAGCAGCC GGGTGTGTAA GAACTGCGGT TGTACCGATA TAGACGTAGA TGCGGCCCGC 
GGGGATGCGG TGTGTACGGG CTGCGGCTCG GTGCTGGAGG ATAATATCAT CGTGTCTGAG 
GTGCAGTTCG TGGAGGGCGC AGGCGGGATC TCCTCGGCAG TTGGACAGTT CGTCTCCTCG 
GAATGTTCTG GTAAAACTCC ATCACTGGGA GAAGGATTTT ACACCAGCGT GGGAAAGGAG 
TCCCGTGCAC AAACTCTACA GAATGGGAAG CGTCAGATTC ACAACTTGGG AGTGCAGTTA 
CAGCTAAATA AGCATTGCCT GGACACTGCC TTCAACTTCT TTAAAATGGC TGTCAGCAAG 
CACCTTACCC GTGGTAGGAA GATGACCCAT GTCATAGCCG CCTGCTTGTA CTTGGTCTGT 
AGGACAGAAG GAACTCCCCA TATGTTACTT GATCTCAGTG ACCTTCTTCA GGTGAATGTG 
TATGTCCTTG GCAAGACCTT TCTTCTCTTA GCCAGAGAAC TCTGCATCAA TGCTCCTGCT 
ATAGACCCCT GCTTATATAT TCCTCGATTT GCACACTTGC TGGAGTTTGG CGATAAAAAT 
CATGAAGTAT CAATGACTGC ACTGAGGTTG GTCCAAAGGA TGAAAAGAGA TTGGATGCAC 
ACTGGGCGCC GCCCATCTGG ACTCTGCGGA GCTGCACTGC TTGTTGCATC CAGAATGCAT 
GATTTCCGGC GGACCATTAA AGAGGTCATC AGGGTGGTAA AGGTGTGTGA GTCTACACTG 
AGGAAAAGAT TAACAGAATT TGAAGATACT CCAACAAGTC AACTGACAAT TGATGAATTT 
ATGAAGATTG ATTTGGAACA AGAGTGTGAT CCTCCTTCCT TTACTGCTGG TCAAAGGAAG 
GTTAAAATAC AACAGCTTGA GCAAGAGTTG GCAAAAAAAC TTGATGAAGT AGAAGGGGAA 
ATATCAAGTT ATCAAGATGA AATAGAGAAT GAGCTGGAGA GTAGCAGACC AAAGCCCAAA 
GGAATATATG CTTCATATGC TAAAAATGGT TCAGAAGACA ACACTTCTAG CATACTTGCA 
GAAGAAGATG ATGATGAAGA CCTGGAAGCA GCCGCTAATC ACTTAAACAA GGATTTTTAC 
AGTGAACTTG CTAATGATAC ATCTAAAGGG ATTTGTTATG AGCACACCGT GAAGCCACTT 
CCTATAGAAT CTCTACTAGG CCCTCTCCCC ACTGCTGCAA GCTTGGGGAT TACAGACTCC 
ATCAAGGAAT GCATAGCAAT AAATGAGCAA GAGAAAAGTG ACAATGCAGG AGACGGCGAA 
CTCGACCTTA ATGGAATTGA TGACAATGAA ATAGACAGGT ATATTCTCAA TGAGAAGGAA 
GCAGAAATAA AGAAAGAGAT GTGGATGAAA GAGAATGAGG ACTACCTTCG AGAACAAAAG 
GAAAAGGAAG CGCGTATAGC TAAAGAGAAG GAGCTTGGTA TTTACAAAGA GCAAAAGCCA 
AGGAAACCTT CCAAGCGGAG GGCTCCAATC CAAGCCAGCA CTGCTGGGGA GGCAATTGAA 
AAGATGCTGG AGCAGAAGAA AATCTCCAGT AAAATTAACT ATGATGTACT CCGGGACCTC 
AACAGCAAAG GGAGCAGTAC TCCAAAGCAC GATAAGAACG ATATGGAGGA AGGGATCACT 
AGCAACCAAC TCTCCCGCAG GAAATCTGCT GCCAGTAAAA TTCTGTCCAA TCCCGTCAGC 
AACGCCGGCA AAAGGATAAG ACCACTGGTT TCAACGCAGC CATCTAAGAA AGTAGCATTT 
GAACAGGAAG TCAGAAATGA ACTTGAGTCA ACACCCCCAA AGATAGTAGT GGAGAGTGGG 
CCAGTCACAT ACAATCACGA GGATGATGCC GCCGCCGCCG AAGAAGAAGA GGAGGAAGAG 
GAAGAACACT GTGTGAGCGC ACTTCAGTTA ATGGGGGGAA ATGATTACGG CTGTGATATG 
GATGAGGATG ATTACTGA