nisch cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following nisch gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nisch cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa55830 XM_031899905.1
Latest version!
Xenopus tropicalis nischarin (nisch), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OXa55825 XM_031899900.1
Latest version!
Xenopus tropicalis nischarin (nisch), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa55826 XM_031899901.1
Latest version!
Xenopus tropicalis nischarin (nisch), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa55827 XM_031899902.1
Latest version!
Xenopus tropicalis nischarin (nisch), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa55828 XM_031899903.1
Latest version!
Xenopus tropicalis nischarin (nisch), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa55829 XM_031899904.1
Latest version!
Xenopus tropicalis nischarin (nisch), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa23481 NM_001130345.1
Latest version!
Xenopus tropicalis nischarin (nisch), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa55830
    Clone ID Related Accession (Same CDS sequence) XM_031899905.1
    Accession Version XM_031899905.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1401bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nischarin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGAGGACG AGGAGGCAGA GGAACGACGG GAGGTCCGGG TTGTGGGCTC CGAACTGGTG 
    GAGAACTACA CGGTTTATAT CATTCAGCTC CGCGCTGGGA GTTTCCACTG GGAAGTGAGA
    CACCGATACA GCGACTTCTA TGATCTCCAT GAGAAGTTGA CGGCGGAGAA TAAAATCGAG
    AGGAACCTTT TGCCCCCGAA GAAGATGATC GGGAAGAACT CGAGAAGTTT GGTGGAAAAG
    CGGCAGAAAG ATCTGGAATT GTATCTGCAG ACGCTGCTTG GCACGTTCCC CCTCGCTGTG
    CCCAAAGCCC TGGCACGCTT CCTGCATTTC CACCTCTATG AAATCCATGG AATCGCTGCA
    GTTCTGGCCG AGGAGCTTTT CCATAAAGGG GAGCAGTTGC TATTGGCCGG AGAGGTATTT
    ACCGTGACCC CACTGCAGCT CTATGCCGTG ACGCAGTTAC TCCGACGCGC CAAGCCCACC
    TGTAGCACCG GCGACGCCAG GAACGACCTG GGGCACATCA TGGATTTTGC TTGTAGACTG
    AAATACCTCC GGATCAGCGG CCGGACCGGT CCTGTTGGGA CGAGTAATAT CCATGAGCAG
    TCTCTTCCCT GTGACCTCTC CATCTTCAAA TCCCTCTGTC AGATTGAGGT CAGTCAGTGC
    AACGCCCGGC TCCTCAGCGG CCTCACTTCC TGTAGGAGGA GTTTAGCCAC CATCAGCATC
    CACTGCTCCG CCTCTTCCAT GAAGGACATT CTGGTGCCGG AGGCCTATGA CTTTGAGCAG
    TGGGAGCCGG AGGGAGTGGC CCCTGGGAGC CCCATTACTG CAGTTGTGCC CAAGTGGAAA
    GTGCTCACAA CTCTGGATCT GAGCCACAAT CGGATCTCCT GTATCGATGA GTCTGTTAAA
    CTGATTCCTG AGATTGAATT CTTGGATTTC AGTCACAACG ACATCTCTAC AATAGAAAAT
    CTGCAGCACC TGTACAACCT CATTCACTTG GACCTGTCCT ACAACAAACT GGCAGACCTG
    ACTGGCATCT ACACTAAGGT TGGGAATATC AAGACTCTCA GTCTGGCCGG GAACGTACTG
    GAAAGTCTCC GCGGCCTCAA TAAATTGTAT TCACTTGTTA ACCTGGATCT GAGCCAGAAC
    AGAATTGAGC AGTTGGAAGA AGTAAGGAAC ATCGGGGGGC TGCCGTGCCT GGAGGGGGTG
    CTCCTCGCTG GGAACCCTCT CACTGTCATT CCGGATTACA GGACCAAAGT TCTGGCGCTG
    TTTGGGGACA GAGCGTCTGA GGTCTGTTTG GACAGTACCA GAACCACAGA GAAAGAGCTG
    GACACGGTGG AAGTGCTGAA GGCCATACAG AAATCAAGGG ACGCACGGAA TAAATTGGCT
    TCTTCTGAGA AAAAGCAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031755765.1
    CDS527..1927
    Translation

    Target ORF information:

    RefSeq Version XM_031899905.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis nischarin (nisch), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899905.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGAGGACG AGGAGGCAGA GGAACGACGG GAGGTCCGGG TTGTGGGCTC CGAACTGGTG 
    GAGAACTACA CGGTTTATAT CATTCAGCTC CGCGCTGGGA GTTTCCACTG GGAAGTGAGA
    CACCGATACA GCGACTTCTA TGATCTCCAT GAGAAGTTGA CGGCGGAGAA TAAAATCGAG
    AGGAACCTTT TGCCCCCGAA GAAGATGATC GGGAAGAACT CGAGAAGTTT GGTGGAAAAG
    CGGCAGAAAG ATCTGGAATT GTATCTGCAG ACGCTGCTTG GCACGTTCCC CCTCGCTGTG
    CCCAAAGCCC TGGCACGCTT CCTGCATTTC CACCTCTATG AAATCCATGG AATCGCTGCA
    GTTCTGGCCG AGGAGCTTTT CCATAAAGGG GAGCAGTTGC TATTGGCCGG AGAGGTATTT
    ACCGTGACCC CACTGCAGCT CTATGCCGTG ACGCAGTTAC TCCGACGCGC CAAGCCCACC
    TGTAGCACCG GCGACGCCAG GAACGACCTG GGGCACATCA TGGATTTTGC TTGTAGACTG
    AAATACCTCC GGATCAGCGG CCGGACCGGT CCTGTTGGGA CGAGTAATAT CCATGAGCAG
    TCTCTTCCCT GTGACCTCTC CATCTTCAAA TCCCTCTGTC AGATTGAGGT CAGTCAGTGC
    AACGCCCGGC TCCTCAGCGG CCTCACTTCC TGTAGGAGGA GTTTAGCCAC CATCAGCATC
    CACTGCTCCG CCTCTTCCAT GAAGGACATT CTGGTGCCGG AGGCCTATGA CTTTGAGCAG
    TGGGAGCCGG AGGGAGTGGC CCCTGGGAGC CCCATTACTG CAGTTGTGCC CAAGTGGAAA
    GTGCTCACAA CTCTGGATCT GAGCCACAAT CGGATCTCCT GTATCGATGA GTCTGTTAAA
    CTGATTCCTG AGATTGAATT CTTGGATTTC AGTCACAACG ACATCTCTAC AATAGAAAAT
    CTGCAGCACC TGTACAACCT CATTCACTTG GACCTGTCCT ACAACAAACT GGCAGACCTG
    ACTGGCATCT ACACTAAGGT TGGGAATATC AAGACTCTCA GTCTGGCCGG GAACGTACTG
    GAAAGTCTCC GCGGCCTCAA TAAATTGTAT TCACTTGTTA ACCTGGATCT GAGCCAGAAC
    AGAATTGAGC AGTTGGAAGA AGTAAGGAAC ATCGGGGGGC TGCCGTGCCT GGAGGGGGTG
    CTCCTCGCTG GGAACCCTCT CACTGTCATT CCGGATTACA GGACCAAAGT TCTGGCGCTG
    TTTGGGGACA GAGCGTCTGA GGTCTGTTTG GACAGTACCA GAACCACAGA GAAAGAGCTG
    GACACGGTGG AAGTGCTGAA GGCCATACAG AAATCAAGGG ACGCACGGAA TAAATTGGCT
    TCTTCTGAGA AAAAGCAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55825
    Clone ID Related Accession (Same CDS sequence) XM_031899900.1
    Accession Version XM_031899900.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4089bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nischarin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAGGACG AGGAGGCAGA GGAACGACGG GAGGTCCGGG TTGTGGGCTC CGAACTGGTG 
    GAGAACTACA CGGTTTATAT CATTCAGCTC CGCGCTGGGA GTTTCCACTG GGAAGTGAGA
    CACCGATACA GCGACTTCTA TGATCTCCAT GAGAAGTTGA CGGCGGAGAA TAAAATCGAG
    AGGAACCTTT TGCCCCCGAA GAAGATGATC GGGAAGAACT CGAGAAGTTT GGTGGAAAAG
    CGGCAGAAAG ATCTGGAATT GTATCTGCAG ACGCTGCTTG GCACGTTCCC CCTCGCTGTG
    CCCAAAGCCC TGGCACGCTT CCTGCATTTC CACCTCTATG AAATCCATGG AATCGCTGCA
    GTTCTGGCCG AGGAGCTTTT CCATAAAGGG GAGCAGTTGC TATTGGCCGG AGAGGTATTT
    ACCGTGACCC CACTGCAGCT CTATGCCGTG ACGCAGTTAC TCCGACGCGC CAAGCCCACC
    TGTAGCACCG GCGACGCCAG GAACGACCTG GGGCACATCA TGGATTTTGC TTGTAGACTG
    AAATACCTCC GGATCAGCGG CCGGACCGGT CCTGTTGGGA CGAGTAATAT CCATGAGCAG
    TCTCTTCCCT GTGACCTCTC CATCTTCAAA TCCCTCTGTC AGATTGAGGT CAGTCAGTGC
    AACGCCCGGC TCCTCAGCGG CCTCACTTCC TGTAGGAGGA GTTTAGCCAC CATCAGCATC
    CACTGCTCCG CCTCTTCCAT GAAGGACATT CTGGTGCCGG AGGCCTATGA CTTTGAGCAG
    TGGGAGCCGG AGGGAGTGGC CCCTGGGAGC CCCATTACTG CAGTTGTGCC CAAGTGGAAA
    GTGCTCACAA CTCTGGATCT GAGCCACAAT CGGATCTCCT GTATCGATGA GTCTGTTAAA
    CTGATTCCTG AGATTGAATT CTTGGATTTC AGTCACAACG ACATCTCTAC AATAGAAAAT
    CTGCAGCACC TGTACAACCT CATTCACTTG GACCTGTCCT ACAACAAACT GGCAGACCTG
    ACTGGCATCT ACACTAAGGT TGGGAATATC AAGACTCTCA GTCTGGCCGG GAACGTACTG
    GAAAGTCTCC GCGGCCTCAA TAAATTGTAT TCACTTGTTA ACCTGGATCT GAGCCAGAAC
    AGAATTGAGC AGTTGGAAGA AGTAAGGAAC ATCGGGGGGC TGCCGTGCCT GGAGGGGGTG
    CTCCTCGCTG GGAACCCTCT CACTGTCATT CCGGATTACA GGACCAAAGT TCTGGCGCTG
    TTTGGGGACA GAGCGTCTGA GGTCTGTTTG GACAGTACCA GAACCACAGA GAAAGAGCTG
    GACACGGTGG AAGTGCTGAA GGCCATACAG AAATCAAGGG ACGCACGGAA TAAATTGGCT
    TCTTCTGAGA AAAAGATCAA TGAGGAATTG CGACCCTCTG CTGCTGGCTC TTCCTCCCCT
    GCCCGTGCTT CCTCTTCCTC TCTCTCTCGG CCTATTAGCT GCAGCCAAGG TGCAGATTCT
    ATAGACCTTT CTTTAGCGAA AAGACTGTCC CCAGGTGGAG CCCAACAGAG TGGCTCTGGG
    GACCCGGCGG CCAAGCAAGA AGCGGAGAGA GGGAAGCCGT GTGAGGGGGA TGTACAGAGA
    GACGTGTCTG ACCCTCCCCC GGGCAGTGGG ACCAGTTGCC GGGAAGATGC CGTTACGTTG
    GACCAATGGG GCCCCTGTCC CTGTATGTCG CTGTATCAGG GGGACAAGGA GCTGCTCCCC
    TACATTTCTG TGCTGATGGA GGGCGCATTG AGGGAACCCC GACATGTCGC TGGGGAGGCG
    GAGAATAAGT GGGGGCCCCA CAGTTGCGAG TTCCATAAGA CAGAAGACGG TTACTTTGAG
    ATGGGGCCGG ATACCCAAAC GACAGATGGA ACCTTCCCAG CTGCCCCGGC TGTCAGTCAC
    ATAGTGTGGG GTCTCTCGTT CCACAGTCGG GGGGCAGCGG AGCTCAGACC CTTCGCCTCG
    TGCCTTGTGC TCACCGATAC CCTCGTCGTC TGCTTTGAGC TGGCGCCCGA CTCCGCCCCC
    AACTTACTGC GACTGGTTCT GTGTTTCGCC TATGGGGACG TCGCAGGATT TCATTTCCCC
    GTCCCGGACA TTTGCCTGAG AGTCAGTTTG GGGGACGGGG AGGAGAGTGT CTTTGTTATG
    TCAGACTTGC AGGGCTTGCA GCAACTCTCT GCCTGTTTGA AAGCCCGCTG CCCCCCAAGC
    CACACCCCTG AGAGGATTAT TCCTGAACCA ATGAGCTTGC AGGATTTGCT CCTTTATATT
    TCCGAGGTGC AGCAAGTCGT ACTGAGCGAT AGCGACGTAA AGGGATGTTT CCCGGTGTAT
    CTGTTTAACG GTGAGGCCGC CGGTGACCCC GCCCAACCAG AGCCTCCATT TCATACACAA
    ACATGGCCTC CAGCTGCCTC CTGTGCTGCA CTCCTTTCCA TCATATCTAC AGGCCTTCAG
    CCCGGGGGGG AAAGGCTGCT CCCCCATTGG CTACTCCTCA CTCAGCAATG TTTCATACTA
    ATTACAGTCG ACATTAGTAC AATAACCGGT AACCGGGCGG AGTACGCGGG AAATGCGTTA
    ACGATGAGCG CCATTCCCTT GGCTTCGGTT GCGTTGGAGC CGGAGAGTCC CCCTGCTGAG
    AATAAGCCCC CCCAGTTTGC CGGACACACT GTCCCTTTAA TGGTGGATTA CAGAGCAGTC
    AGCGCATTCT TTGTTGTGCC CCAAAATAAA TTCCACTTCT TGTCGGTGTT TAACCAGCTG
    AGAAAGTCCC TTCAGGGGAT AAAGAACTTG GTGATTTGCA AATCCATAAA AAATCTGGAC
    GATTCCCAGA GCTGGGTGGG CGACGCCCAC AGACCCCCAA CGCACTCGCT AAGCCCCGTT
    GCGCCCCACA GGGCACGGCC CCCCCAGTAC CCATCAGAGA GCCTGACACA GAGATTATCA
    GAGGAAAACC ACATTCCCTT CTACCTTCCT GCATCGGCCT CGCTGCGCTT CATTGCTGAA
    CTGAAGGGGA AGGCGCTGGT GGAGCTGTTC CGTAGCTGCG TTGCCGAGGC AGAGGGGGAG
    GAGCTGAGGC ATCTCACGTG GGCATCACTG CTGCTTTATG GGACCCCCGA TACAGAGTTT
    CCATCCTGCA TTTTACTTTC AACACAAGCT GTTTATTTTC TCTTGGATGA TACTCAGAAC
    CCCAAAGAAG AAGATTATTG GAGGAAGAGA CCAGCGCGCA GCCCCCTCCA GATTTCCTTC
    TGTTTCGCCA TCAAATTCAG CAGTTTACAG TCCGTCAATA TCGGACTTTT TGATCAGTAT
    TTCAGGATAA CAGGTTCTTC CCCCAGTCAG GTGGTCACGT GTCTGACCAG GGACAGTTAC
    AGCACCCACT CCTTCATCCA GCAGCTCATG TCTGCCCTGT CCCTCCTGGC ACGGGCCCCG
    TCCCCCGAAC CCCTGGAGAA GGATTTCTAC TCGGAATTTG GTACAAAGAA CACGGGGAGA
    ATGGAAAACT ACGAACTGAT CCATTCCAGC AGAGTGAAAT TCGTTTACCC AAACGAGGAG
    GAGATGGGAG ACCTGACGTT CATAGTGTCG GAGAAGATGG ACCCTTCCAG CCCCATCCAG
    TCGCTAAACA TTCTGCTGTA CGTATTGGGC TTCCGGGTCT CTTGTGTGGG CAGAACCGAC
    CCGCCGCCCT CCCTGCAGCC CAACAGCCTC ATCCTCACCA GCTCCGACCT GTTTCTGTTC
    AATGAGGATT ATATCAGTTA CCCGCTGCCA GACTTTGCCA AGGAACCCCC CAAGAAGGAC
    AAATACCAGT TAGTTGATGG GAGGAGAATC AGGGATTTGG ACCGGGTGCT GATGGGATAC
    CAGACCTACC CTCAGAACCT CACCTTGGTG TTTGATGATG TTCACAAGCA GGATTTCATG
    CACAGCGTCT CTCTCGATCA CTTTGGGGAG ACGCTGGGGG GCTCTCCCGG GGCTGTGGGA
    GCCAATAAAG AGATCCAGTG GAACATTTTT ATCCCAAGCG CAGACTCCCG GGAGAAGCTG
    ATCTCTCTGC TCGCCCGCCA ATGGGAGAGC TTGTGCGGCA GGGAGCTTCC CATCGAACTC
    ACCGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031755760.1
    CDS565..4653
    Translation

    Target ORF information:

    RefSeq Version XM_031899900.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis nischarin (nisch), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899900.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAGGACG AGGAGGCAGA GGAACGACGG GAGGTCCGGG TTGTGGGCTC CGAACTGGTG 
    GAGAACTACA CGGTTTATAT CATTCAGCTC CGCGCTGGGA GTTTCCACTG GGAAGTGAGA
    CACCGATACA GCGACTTCTA TGATCTCCAT GAGAAGTTGA CGGCGGAGAA TAAAATCGAG
    AGGAACCTTT TGCCCCCGAA GAAGATGATC GGGAAGAACT CGAGAAGTTT GGTGGAAAAG
    CGGCAGAAAG ATCTGGAATT GTATCTGCAG ACGCTGCTTG GCACGTTCCC CCTCGCTGTG
    CCCAAAGCCC TGGCACGCTT CCTGCATTTC CACCTCTATG AAATCCATGG AATCGCTGCA
    GTTCTGGCCG AGGAGCTTTT CCATAAAGGG GAGCAGTTGC TATTGGCCGG AGAGGTATTT
    ACCGTGACCC CACTGCAGCT CTATGCCGTG ACGCAGTTAC TCCGACGCGC CAAGCCCACC
    TGTAGCACCG GCGACGCCAG GAACGACCTG GGGCACATCA TGGATTTTGC TTGTAGACTG
    AAATACCTCC GGATCAGCGG CCGGACCGGT CCTGTTGGGA CGAGTAATAT CCATGAGCAG
    TCTCTTCCCT GTGACCTCTC CATCTTCAAA TCCCTCTGTC AGATTGAGGT CAGTCAGTGC
    AACGCCCGGC TCCTCAGCGG CCTCACTTCC TGTAGGAGGA GTTTAGCCAC CATCAGCATC
    CACTGCTCCG CCTCTTCCAT GAAGGACATT CTGGTGCCGG AGGCCTATGA CTTTGAGCAG
    TGGGAGCCGG AGGGAGTGGC CCCTGGGAGC CCCATTACTG CAGTTGTGCC CAAGTGGAAA
    GTGCTCACAA CTCTGGATCT GAGCCACAAT CGGATCTCCT GTATCGATGA GTCTGTTAAA
    CTGATTCCTG AGATTGAATT CTTGGATTTC AGTCACAACG ACATCTCTAC AATAGAAAAT
    CTGCAGCACC TGTACAACCT CATTCACTTG GACCTGTCCT ACAACAAACT GGCAGACCTG
    ACTGGCATCT ACACTAAGGT TGGGAATATC AAGACTCTCA GTCTGGCCGG GAACGTACTG
    GAAAGTCTCC GCGGCCTCAA TAAATTGTAT TCACTTGTTA ACCTGGATCT GAGCCAGAAC
    AGAATTGAGC AGTTGGAAGA AGTAAGGAAC ATCGGGGGGC TGCCGTGCCT GGAGGGGGTG
    CTCCTCGCTG GGAACCCTCT CACTGTCATT CCGGATTACA GGACCAAAGT TCTGGCGCTG
    TTTGGGGACA GAGCGTCTGA GGTCTGTTTG GACAGTACCA GAACCACAGA GAAAGAGCTG
    GACACGGTGG AAGTGCTGAA GGCCATACAG AAATCAAGGG ACGCACGGAA TAAATTGGCT
    TCTTCTGAGA AAAAGATCAA TGAGGAATTG CGACCCTCTG CTGCTGGCTC TTCCTCCCCT
    GCCCGTGCTT CCTCTTCCTC TCTCTCTCGG CCTATTAGCT GCAGCCAAGG TGCAGATTCT
    ATAGACCTTT CTTTAGCGAA AAGACTGTCC CCAGGTGGAG CCCAACAGAG TGGCTCTGGG
    GACCCGGCGG CCAAGCAAGA AGCGGAGAGA GGGAAGCCGT GTGAGGGGGA TGTACAGAGA
    GACGTGTCTG ACCCTCCCCC GGGCAGTGGG ACCAGTTGCC GGGAAGATGC CGTTACGTTG
    GACCAATGGG GCCCCTGTCC CTGTATGTCG CTGTATCAGG GGGACAAGGA GCTGCTCCCC
    TACATTTCTG TGCTGATGGA GGGCGCATTG AGGGAACCCC GACATGTCGC TGGGGAGGCG
    GAGAATAAGT GGGGGCCCCA CAGTTGCGAG TTCCATAAGA CAGAAGACGG TTACTTTGAG
    ATGGGGCCGG ATACCCAAAC GACAGATGGA ACCTTCCCAG CTGCCCCGGC TGTCAGTCAC
    ATAGTGTGGG GTCTCTCGTT CCACAGTCGG GGGGCAGCGG AGCTCAGACC CTTCGCCTCG
    TGCCTTGTGC TCACCGATAC CCTCGTCGTC TGCTTTGAGC TGGCGCCCGA CTCCGCCCCC
    AACTTACTGC GACTGGTTCT GTGTTTCGCC TATGGGGACG TCGCAGGATT TCATTTCCCC
    GTCCCGGACA TTTGCCTGAG AGTCAGTTTG GGGGACGGGG AGGAGAGTGT CTTTGTTATG
    TCAGACTTGC AGGGCTTGCA GCAACTCTCT GCCTGTTTGA AAGCCCGCTG CCCCCCAAGC
    CACACCCCTG AGAGGATTAT TCCTGAACCA ATGAGCTTGC AGGATTTGCT CCTTTATATT
    TCCGAGGTGC AGCAAGTCGT ACTGAGCGAT AGCGACGTAA AGGGATGTTT CCCGGTGTAT
    CTGTTTAACG GTGAGGCCGC CGGTGACCCC GCCCAACCAG AGCCTCCATT TCATACACAA
    ACATGGCCTC CAGCTGCCTC CTGTGCTGCA CTCCTTTCCA TCATATCTAC AGGCCTTCAG
    CCCGGGGGGG AAAGGCTGCT CCCCCATTGG CTACTCCTCA CTCAGCAATG TTTCATACTA
    ATTACAGTCG ACATTAGTAC AATAACCGGT AACCGGGCGG AGTACGCGGG AAATGCGTTA
    ACGATGAGCG CCATTCCCTT GGCTTCGGTT GCGTTGGAGC CGGAGAGTCC CCCTGCTGAG
    AATAAGCCCC CCCAGTTTGC CGGACACACT GTCCCTTTAA TGGTGGATTA CAGAGCAGTC
    AGCGCATTCT TTGTTGTGCC CCAAAATAAA TTCCACTTCT TGTCGGTGTT TAACCAGCTG
    AGAAAGTCCC TTCAGGGGAT AAAGAACTTG GTGATTTGCA AATCCATAAA AAATCTGGAC
    GATTCCCAGA GCTGGGTGGG CGACGCCCAC AGACCCCCAA CGCACTCGCT AAGCCCCGTT
    GCGCCCCACA GGGCACGGCC CCCCCAGTAC CCATCAGAGA GCCTGACACA GAGATTATCA
    GAGGAAAACC ACATTCCCTT CTACCTTCCT GCATCGGCCT CGCTGCGCTT CATTGCTGAA
    CTGAAGGGGA AGGCGCTGGT GGAGCTGTTC CGTAGCTGCG TTGCCGAGGC AGAGGGGGAG
    GAGCTGAGGC ATCTCACGTG GGCATCACTG CTGCTTTATG GGACCCCCGA TACAGAGTTT
    CCATCCTGCA TTTTACTTTC AACACAAGCT GTTTATTTTC TCTTGGATGA TACTCAGAAC
    CCCAAAGAAG AAGATTATTG GAGGAAGAGA CCAGCGCGCA GCCCCCTCCA GATTTCCTTC
    TGTTTCGCCA TCAAATTCAG CAGTTTACAG TCCGTCAATA TCGGACTTTT TGATCAGTAT
    TTCAGGATAA CAGGTTCTTC CCCCAGTCAG GTGGTCACGT GTCTGACCAG GGACAGTTAC
    AGCACCCACT CCTTCATCCA GCAGCTCATG TCTGCCCTGT CCCTCCTGGC ACGGGCCCCG
    TCCCCCGAAC CCCTGGAGAA GGATTTCTAC TCGGAATTTG GTACAAAGAA CACGGGGAGA
    ATGGAAAACT ACGAACTGAT CCATTCCAGC AGAGTGAAAT TCGTTTACCC AAACGAGGAG
    GAGATGGGAG ACCTGACGTT CATAGTGTCG GAGAAGATGG ACCCTTCCAG CCCCATCCAG
    TCGCTAAACA TTCTGCTGTA CGTATTGGGC TTCCGGGTCT CTTGTGTGGG CAGAACCGAC
    CCGCCGCCCT CCCTGCAGCC CAACAGCCTC ATCCTCACCA GCTCCGACCT GTTTCTGTTC
    AATGAGGATT ATATCAGTTA CCCGCTGCCA GACTTTGCCA AGGAACCCCC CAAGAAGGAC
    AAATACCAGT TAGTTGATGG GAGGAGAATC AGGGATTTGG ACCGGGTGCT GATGGGATAC
    CAGACCTACC CTCAGAACCT CACCTTGGTG TTTGATGATG TTCACAAGCA GGATTTCATG
    CACAGCGTCT CTCTCGATCA CTTTGGGGAG ACGCTGGGGG GCTCTCCCGG GGCTGTGGGA
    GCCAATAAAG AGATCCAGTG GAACATTTTT ATCCCAAGCG CAGACTCCCG GGAGAAGCTG
    ATCTCTCTGC TCGCCCGCCA ATGGGAGAGC TTGTGCGGCA GGGAGCTTCC CATCGAACTC
    ACCGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55826
    Clone ID Related Accession (Same CDS sequence) XM_031899901.1
    Accession Version XM_031899901.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3822bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nischarin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGACG AGGAGGCAGA GGAACGACGG GAGGTCCGGG TTGTGGGCTC CGAACTGGTG 
    GAGAACTACA CGGAAATCCA TGGAATCGCT GCAGTTCTGG CCGAGGAGCT TTTCCATAAA
    GGGGAGCAGT TGCTATTGGC CGGAGAGGTA TTTACCGTGA CCCCACTGCA GCTCTATGCC
    GTGACGCAGT TACTCCGACG CGCCAAGCCC ACCTGTAGCA CCGGCGACGC CAGGAACGAC
    CTGGGGCACA TCATGGATTT TGCTTGTAGA CTGAAATACC TCCGGATCAG CGGCCGGACC
    GGTCCTGTTG GGACGAGTAA TATCCATGAG CAGTCTCTTC CCTGTGACCT CTCCATCTTC
    AAATCCCTCT GTCAGATTGA GGTCAGTCAG TGCAACGCCC GGCTCCTCAG CGGCCTCACT
    TCCTGTAGGA GGAGTTTAGC CACCATCAGC ATCCACTGCT CCGCCTCTTC CATGAAGGAC
    ATTCTGGTGC CGGAGGCCTA TGACTTTGAG CAGTGGGAGC CGGAGGGAGT GGCCCCTGGG
    AGCCCCATTA CTGCAGTTGT GCCCAAGTGG AAAGTGCTCA CAACTCTGGA TCTGAGCCAC
    AATCGGATCT CCTGTATCGA TGAGTCTGTT AAACTGATTC CTGAGATTGA ATTCTTGGAT
    TTCAGTCACA ACGACATCTC TACAATAGAA AATCTGCAGC ACCTGTACAA CCTCATTCAC
    TTGGACCTGT CCTACAACAA ACTGGCAGAC CTGACTGGCA TCTACACTAA GGTTGGGAAT
    ATCAAGACTC TCAGTCTGGC CGGGAACGTA CTGGAAAGTC TCCGCGGCCT CAATAAATTG
    TATTCACTTG TTAACCTGGA TCTGAGCCAG AACAGAATTG AGCAGTTGGA AGAAGTAAGG
    AACATCGGGG GGCTGCCGTG CCTGGAGGGG GTGCTCCTCG CTGGGAACCC TCTCACTGTC
    ATTCCGGATT ACAGGACCAA AGTTCTGGCG CTGTTTGGGG ACAGAGCGTC TGAGGTCTGT
    TTGGACAGTA CCAGAACCAC AGAGAAAGAG CTGGACACGG TGGAAGTGCT GAAGGCCATA
    CAGAAATCAA GGGACGCACG GAATAAATTG GCTTCTTCTG AGAAAAAGAT CAATGAGGAA
    TTGCGACCCT CTGCTGCTGG CTCTTCCTCC CCTGCCCGTG CTTCCTCTTC CTCTCTCTCT
    CGGCCTATTA GCTGCAGCCA AGGTGCAGAT TCTATAGACC TTTCTTTAGC GAAAAGACTG
    TCCCCAGGTG GAGCCCAACA GAGTGGCTCT GGGGACCCGG CGGCCAAGCA AGAAGCGGAG
    AGAGGGAAGC CGTGTGAGGG GGATGTACAG AGAGACGTGT CTGACCCTCC CCCGGGCAGT
    GGGACCAGTT GCCGGGAAGA TGCCGTTACG TTGGACCAAT GGGGCCCCTG TCCCTGTATG
    TCGCTGTATC AGGGGGACAA GGAGCTGCTC CCCTACATTT CTGTGCTGAT GGAGGGCGCA
    TTGAGGGAAC CCCGACATGT CGCTGGGGAG GCGGAGAATA AGTGGGGGCC CCACAGTTGC
    GAGTTCCATA AGACAGAAGA CGGTTACTTT GAGATGGGGC CGGATACCCA AACGACAGAT
    GGAACCTTCC CAGCTGCCCC GGCTGTCAGT CACATAGTGT GGGGTCTCTC GTTCCACAGT
    CGGGGGGCAG CGGAGCTCAG ACCCTTCGCC TCGTGCCTTG TGCTCACCGA TACCCTCGTC
    GTCTGCTTTG AGCTGGCGCC CGACTCCGCC CCCAACTTAC TGCGACTGGT TCTGTGTTTC
    GCCTATGGGG ACGTCGCAGG ATTTCATTTC CCCGTCCCGG ACATTTGCCT GAGAGTCAGT
    TTGGGGGACG GGGAGGAGAG TGTCTTTGTT ATGTCAGACT TGCAGGGCTT GCAGCAACTC
    TCTGCCTGTT TGAAAGCCCG CTGCCCCCCA AGCCACACCC CTGAGAGGAT TATTCCTGAA
    CCAATGAGCT TGCAGGATTT GCTCCTTTAT ATTTCCGAGG TGCAGCAAGT CGTACTGAGC
    GATAGCGACG TAAAGGGATG TTTCCCGGTG TATCTGTTTA ACGGTGAGGC CGCCGGTGAC
    CCCGCCCAAC CAGAGCCTCC ATTTCATACA CAAACATGGC CTCCAGCTGC CTCCTGTGCT
    GCACTCCTTT CCATCATATC TACAGGCCTT CAGCCCGGGG GGGAAAGGCT GCTCCCCCAT
    TGGCTACTCC TCACTCAGCA ATGTTTCATA CTAATTACAG TCGACATTAG TACAATAACC
    GGTAACCGGG CGGAGTACGC GGGAAATGCG TTAACGATGA GCGCCATTCC CTTGGCTTCG
    GTTGCGTTGG AGCCGGAGAG TCCCCCTGCT GAGAATAAGC CCCCCCAGTT TGCCGGACAC
    ACTGTCCCTT TAATGGTGGA TTACAGAGCA GTCAGCGCAT TCTTTGTTGT GCCCCAAAAT
    AAATTCCACT TCTTGTCGGT GTTTAACCAG CTGAGAAAGT CCCTTCAGGG GATAAAGAAC
    TTGGTGATTT GCAAATCCAT AAAAAATCTG GACGATTCCC AGAGCTGGGT GGGCGACGCC
    CACAGACCCC CAACGCACTC GCTAAGCCCC GTTGCGCCCC ACAGGGCACG GCCCCCCCAG
    TACCCATCAG AGAGCCTGAC ACAGAGATTA TCAGAGGAAA ACCACATTCC CTTCTACCTT
    CCTGCATCGG CCTCGCTGCG CTTCATTGCT GAACTGAAGG GGAAGGCGCT GGTGGAGCTG
    TTCCGTAGCT GCGTTGCCGA GGCAGAGGGG GAGGAGCTGA GGCATCTCAC GTGGGCATCA
    CTGCTGCTTT ATGGGACCCC CGATACAGAG TTTCCATCCT GCATTTTACT TTCAACACAA
    GCTGTTTATT TTCTCTTGGA TGATACTCAG AACCCCAAAG AAGAAGATTA TTGGAGGAAG
    AGACCAGCGC GCAGCCCCCT CCAGATTTCC TTCTGTTTCG CCATCAAATT CAGCAGTTTA
    CAGTCCGTCA ATATCGGACT TTTTGATCAG TATTTCAGGA TAACAGGTTC TTCCCCCAGT
    CAGGTGGTCA CGTGTCTGAC CAGGGACAGT TACAGCACCC ACTCCTTCAT CCAGCAGCTC
    ATGTCTGCCC TGTCCCTCCT GGCACGGGCC CCGTCCCCCG AACCCCTGGA GAAGGATTTC
    TACTCGGAAT TTGGTACAAA GAACACGGGG AGAATGGAAA ACTACGAACT GATCCATTCC
    AGCAGAGTGA AATTCGTTTA CCCAAACGAG GAGGAGATGG GAGACCTGAC GTTCATAGTG
    TCGGAGAAGA TGGACCCTTC CAGCCCCATC CAGTCGCTAA ACATTCTGCT GTACGTATTG
    GGCTTCCGGG TCTCTTGTGT GGGCAGAACC GACCCGCCGC CCTCCCTGCA GCCCAACAGC
    CTCATCCTCA CCAGCTCCGA CCTGTTTCTG TTCAATGAGG ATTATATCAG TTACCCGCTG
    CCAGACTTTG CCAAGGAACC CCCCAAGAAG GACAAATACC AGTTAGTTGA TGGGAGGAGA
    ATCAGGGATT TGGACCGGGT GCTGATGGGA TACCAGACCT ACCCTCAGAA CCTCACCTTG
    GTGTTTGATG ATGTTCACAA GCAGGATTTC ATGCACAGCG TCTCTCTCGA TCACTTTGGG
    GAGACGCTGG GGGGCTCTCC CGGGGCTGTG GGAGCCAATA AAGAGATCCA GTGGAACATT
    TTTATCCCAA GCGCAGACTC CCGGGAGAAG CTGATCTCTC TGCTCGCCCG CCAATGGGAG
    AGCTTGTGCG GCAGGGAGCT TCCCATCGAA CTCACCGGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031755761.1
    CDS566..4387
    Translation

    Target ORF information:

    RefSeq Version XM_031899901.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis nischarin (nisch), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899901.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGACG AGGAGGCAGA GGAACGACGG GAGGTCCGGG TTGTGGGCTC CGAACTGGTG 
    GAGAACTACA CGGAAATCCA TGGAATCGCT GCAGTTCTGG CCGAGGAGCT TTTCCATAAA
    GGGGAGCAGT TGCTATTGGC CGGAGAGGTA TTTACCGTGA CCCCACTGCA GCTCTATGCC
    GTGACGCAGT TACTCCGACG CGCCAAGCCC ACCTGTAGCA CCGGCGACGC CAGGAACGAC
    CTGGGGCACA TCATGGATTT TGCTTGTAGA CTGAAATACC TCCGGATCAG CGGCCGGACC
    GGTCCTGTTG GGACGAGTAA TATCCATGAG CAGTCTCTTC CCTGTGACCT CTCCATCTTC
    AAATCCCTCT GTCAGATTGA GGTCAGTCAG TGCAACGCCC GGCTCCTCAG CGGCCTCACT
    TCCTGTAGGA GGAGTTTAGC CACCATCAGC ATCCACTGCT CCGCCTCTTC CATGAAGGAC
    ATTCTGGTGC CGGAGGCCTA TGACTTTGAG CAGTGGGAGC CGGAGGGAGT GGCCCCTGGG
    AGCCCCATTA CTGCAGTTGT GCCCAAGTGG AAAGTGCTCA CAACTCTGGA TCTGAGCCAC
    AATCGGATCT CCTGTATCGA TGAGTCTGTT AAACTGATTC CTGAGATTGA ATTCTTGGAT
    TTCAGTCACA ACGACATCTC TACAATAGAA AATCTGCAGC ACCTGTACAA CCTCATTCAC
    TTGGACCTGT CCTACAACAA ACTGGCAGAC CTGACTGGCA TCTACACTAA GGTTGGGAAT
    ATCAAGACTC TCAGTCTGGC CGGGAACGTA CTGGAAAGTC TCCGCGGCCT CAATAAATTG
    TATTCACTTG TTAACCTGGA TCTGAGCCAG AACAGAATTG AGCAGTTGGA AGAAGTAAGG
    AACATCGGGG GGCTGCCGTG CCTGGAGGGG GTGCTCCTCG CTGGGAACCC TCTCACTGTC
    ATTCCGGATT ACAGGACCAA AGTTCTGGCG CTGTTTGGGG ACAGAGCGTC TGAGGTCTGT
    TTGGACAGTA CCAGAACCAC AGAGAAAGAG CTGGACACGG TGGAAGTGCT GAAGGCCATA
    CAGAAATCAA GGGACGCACG GAATAAATTG GCTTCTTCTG AGAAAAAGAT CAATGAGGAA
    TTGCGACCCT CTGCTGCTGG CTCTTCCTCC CCTGCCCGTG CTTCCTCTTC CTCTCTCTCT
    CGGCCTATTA GCTGCAGCCA AGGTGCAGAT TCTATAGACC TTTCTTTAGC GAAAAGACTG
    TCCCCAGGTG GAGCCCAACA GAGTGGCTCT GGGGACCCGG CGGCCAAGCA AGAAGCGGAG
    AGAGGGAAGC CGTGTGAGGG GGATGTACAG AGAGACGTGT CTGACCCTCC CCCGGGCAGT
    GGGACCAGTT GCCGGGAAGA TGCCGTTACG TTGGACCAAT GGGGCCCCTG TCCCTGTATG
    TCGCTGTATC AGGGGGACAA GGAGCTGCTC CCCTACATTT CTGTGCTGAT GGAGGGCGCA
    TTGAGGGAAC CCCGACATGT CGCTGGGGAG GCGGAGAATA AGTGGGGGCC CCACAGTTGC
    GAGTTCCATA AGACAGAAGA CGGTTACTTT GAGATGGGGC CGGATACCCA AACGACAGAT
    GGAACCTTCC CAGCTGCCCC GGCTGTCAGT CACATAGTGT GGGGTCTCTC GTTCCACAGT
    CGGGGGGCAG CGGAGCTCAG ACCCTTCGCC TCGTGCCTTG TGCTCACCGA TACCCTCGTC
    GTCTGCTTTG AGCTGGCGCC CGACTCCGCC CCCAACTTAC TGCGACTGGT TCTGTGTTTC
    GCCTATGGGG ACGTCGCAGG ATTTCATTTC CCCGTCCCGG ACATTTGCCT GAGAGTCAGT
    TTGGGGGACG GGGAGGAGAG TGTCTTTGTT ATGTCAGACT TGCAGGGCTT GCAGCAACTC
    TCTGCCTGTT TGAAAGCCCG CTGCCCCCCA AGCCACACCC CTGAGAGGAT TATTCCTGAA
    CCAATGAGCT TGCAGGATTT GCTCCTTTAT ATTTCCGAGG TGCAGCAAGT CGTACTGAGC
    GATAGCGACG TAAAGGGATG TTTCCCGGTG TATCTGTTTA ACGGTGAGGC CGCCGGTGAC
    CCCGCCCAAC CAGAGCCTCC ATTTCATACA CAAACATGGC CTCCAGCTGC CTCCTGTGCT
    GCACTCCTTT CCATCATATC TACAGGCCTT CAGCCCGGGG GGGAAAGGCT GCTCCCCCAT
    TGGCTACTCC TCACTCAGCA ATGTTTCATA CTAATTACAG TCGACATTAG TACAATAACC
    GGTAACCGGG CGGAGTACGC GGGAAATGCG TTAACGATGA GCGCCATTCC CTTGGCTTCG
    GTTGCGTTGG AGCCGGAGAG TCCCCCTGCT GAGAATAAGC CCCCCCAGTT TGCCGGACAC
    ACTGTCCCTT TAATGGTGGA TTACAGAGCA GTCAGCGCAT TCTTTGTTGT GCCCCAAAAT
    AAATTCCACT TCTTGTCGGT GTTTAACCAG CTGAGAAAGT CCCTTCAGGG GATAAAGAAC
    TTGGTGATTT GCAAATCCAT AAAAAATCTG GACGATTCCC AGAGCTGGGT GGGCGACGCC
    CACAGACCCC CAACGCACTC GCTAAGCCCC GTTGCGCCCC ACAGGGCACG GCCCCCCCAG
    TACCCATCAG AGAGCCTGAC ACAGAGATTA TCAGAGGAAA ACCACATTCC CTTCTACCTT
    CCTGCATCGG CCTCGCTGCG CTTCATTGCT GAACTGAAGG GGAAGGCGCT GGTGGAGCTG
    TTCCGTAGCT GCGTTGCCGA GGCAGAGGGG GAGGAGCTGA GGCATCTCAC GTGGGCATCA
    CTGCTGCTTT ATGGGACCCC CGATACAGAG TTTCCATCCT GCATTTTACT TTCAACACAA
    GCTGTTTATT TTCTCTTGGA TGATACTCAG AACCCCAAAG AAGAAGATTA TTGGAGGAAG
    AGACCAGCGC GCAGCCCCCT CCAGATTTCC TTCTGTTTCG CCATCAAATT CAGCAGTTTA
    CAGTCCGTCA ATATCGGACT TTTTGATCAG TATTTCAGGA TAACAGGTTC TTCCCCCAGT
    CAGGTGGTCA CGTGTCTGAC CAGGGACAGT TACAGCACCC ACTCCTTCAT CCAGCAGCTC
    ATGTCTGCCC TGTCCCTCCT GGCACGGGCC CCGTCCCCCG AACCCCTGGA GAAGGATTTC
    TACTCGGAAT TTGGTACAAA GAACACGGGG AGAATGGAAA ACTACGAACT GATCCATTCC
    AGCAGAGTGA AATTCGTTTA CCCAAACGAG GAGGAGATGG GAGACCTGAC GTTCATAGTG
    TCGGAGAAGA TGGACCCTTC CAGCCCCATC CAGTCGCTAA ACATTCTGCT GTACGTATTG
    GGCTTCCGGG TCTCTTGTGT GGGCAGAACC GACCCGCCGC CCTCCCTGCA GCCCAACAGC
    CTCATCCTCA CCAGCTCCGA CCTGTTTCTG TTCAATGAGG ATTATATCAG TTACCCGCTG
    CCAGACTTTG CCAAGGAACC CCCCAAGAAG GACAAATACC AGTTAGTTGA TGGGAGGAGA
    ATCAGGGATT TGGACCGGGT GCTGATGGGA TACCAGACCT ACCCTCAGAA CCTCACCTTG
    GTGTTTGATG ATGTTCACAA GCAGGATTTC ATGCACAGCG TCTCTCTCGA TCACTTTGGG
    GAGACGCTGG GGGGCTCTCC CGGGGCTGTG GGAGCCAATA AAGAGATCCA GTGGAACATT
    TTTATCCCAA GCGCAGACTC CCGGGAGAAG CTGATCTCTC TGCTCGCCCG CCAATGGGAG
    AGCTTGTGCG GCAGGGAGCT TCCCATCGAA CTCACCGGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55827
    Clone ID Related Accession (Same CDS sequence) XM_031899902.1
    Accession Version XM_031899902.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3588bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nischarin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAAATCCA TGGAATCGCT GCAGTTCTGG CCGAGGAGCT TTTCCATAAA GATCAGCGGC 
    CGGACCGGTC CTGTTGGGAC GAGTAATATC CATGAGCAGT CTCTTCCCTG TGACCTCTCC
    ATCTTCAAAT CCCTCTGTCA GATTGAGGTC AGTCAGTGCA ACGCCCGGCT CCTCAGCGGC
    CTCACTTCCT GTAGGAGGAG TTTAGCCACC ATCAGCATCC ACTGCTCCGC CTCTTCCATG
    AAGGACATTC TGGTGCCGGA GGCCTATGAC TTTGAGCAGT GGGAGCCGGA GGGAGTGGCC
    CCTGGGAGCC CCATTACTGC AGTTGTGCCC AAGTGGAAAG TGCTCACAAC TCTGGATCTG
    AGCCACAATC GGATCTCCTG TATCGATGAG TCTGTTAAAC TGATTCCTGA GATTGAATTC
    TTGGATTTCA GTCACAACGA CATCTCTACA ATAGAAAATC TGCAGCACCT GTACAACCTC
    ATTCACTTGG ACCTGTCCTA CAACAAACTG GCAGACCTGA CTGGCATCTA CACTAAGGTT
    GGGAATATCA AGACTCTCAG TCTGGCCGGG AACGTACTGG AAAGTCTCCG CGGCCTCAAT
    AAATTGTATT CACTTGTTAA CCTGGATCTG AGCCAGAACA GAATTGAGCA GTTGGAAGAA
    GTAAGGAACA TCGGGGGGCT GCCGTGCCTG GAGGGGGTGC TCCTCGCTGG GAACCCTCTC
    ACTGTCATTC CGGATTACAG GACCAAAGTT CTGGCGCTGT TTGGGGACAG AGCGTCTGAG
    GTCTGTTTGG ACAGTACCAG AACCACAGAG AAAGAGCTGG ACACGGTGGA AGTGCTGAAG
    GCCATACAGA AATCAAGGGA CGCACGGAAT AAATTGGCTT CTTCTGAGAA AAAGATCAAT
    GAGGAATTGC GACCCTCTGC TGCTGGCTCT TCCTCCCCTG CCCGTGCTTC CTCTTCCTCT
    CTCTCTCGGC CTATTAGCTG CAGCCAAGGT GCAGATTCTA TAGACCTTTC TTTAGCGAAA
    AGACTGTCCC CAGGTGGAGC CCAACAGAGT GGCTCTGGGG ACCCGGCGGC CAAGCAAGAA
    GCGGAGAGAG GGAAGCCGTG TGAGGGGGAT GTACAGAGAG ACGTGTCTGA CCCTCCCCCG
    GGCAGTGGGA CCAGTTGCCG GGAAGATGCC GTTACGTTGG ACCAATGGGG CCCCTGTCCC
    TGTATGTCGC TGTATCAGGG GGACAAGGAG CTGCTCCCCT ACATTTCTGT GCTGATGGAG
    GGCGCATTGA GGGAACCCCG ACATGTCGCT GGGGAGGCGG AGAATAAGTG GGGGCCCCAC
    AGTTGCGAGT TCCATAAGAC AGAAGACGGT TACTTTGAGA TGGGGCCGGA TACCCAAACG
    ACAGATGGAA CCTTCCCAGC TGCCCCGGCT GTCAGTCACA TAGTGTGGGG TCTCTCGTTC
    CACAGTCGGG GGGCAGCGGA GCTCAGACCC TTCGCCTCGT GCCTTGTGCT CACCGATACC
    CTCGTCGTCT GCTTTGAGCT GGCGCCCGAC TCCGCCCCCA ACTTACTGCG ACTGGTTCTG
    TGTTTCGCCT ATGGGGACGT CGCAGGATTT CATTTCCCCG TCCCGGACAT TTGCCTGAGA
    GTCAGTTTGG GGGACGGGGA GGAGAGTGTC TTTGTTATGT CAGACTTGCA GGGCTTGCAG
    CAACTCTCTG CCTGTTTGAA AGCCCGCTGC CCCCCAAGCC ACACCCCTGA GAGGATTATT
    CCTGAACCAA TGAGCTTGCA GGATTTGCTC CTTTATATTT CCGAGGTGCA GCAAGTCGTA
    CTGAGCGATA GCGACGTAAA GGGATGTTTC CCGGTGTATC TGTTTAACGG TGAGGCCGCC
    GGTGACCCCG CCCAACCAGA GCCTCCATTT CATACACAAA CATGGCCTCC AGCTGCCTCC
    TGTGCTGCAC TCCTTTCCAT CATATCTACA GGCCTTCAGC CCGGGGGGGA AAGGCTGCTC
    CCCCATTGGC TACTCCTCAC TCAGCAATGT TTCATACTAA TTACAGTCGA CATTAGTACA
    ATAACCGGTA ACCGGGCGGA GTACGCGGGA AATGCGTTAA CGATGAGCGC CATTCCCTTG
    GCTTCGGTTG CGTTGGAGCC GGAGAGTCCC CCTGCTGAGA ATAAGCCCCC CCAGTTTGCC
    GGACACACTG TCCCTTTAAT GGTGGATTAC AGAGCAGTCA GCGCATTCTT TGTTGTGCCC
    CAAAATAAAT TCCACTTCTT GTCGGTGTTT AACCAGCTGA GAAAGTCCCT TCAGGGGATA
    AAGAACTTGG TGATTTGCAA ATCCATAAAA AATCTGGACG ATTCCCAGAG CTGGGTGGGC
    GACGCCCACA GACCCCCAAC GCACTCGCTA AGCCCCGTTG CGCCCCACAG GGCACGGCCC
    CCCCAGTACC CATCAGAGAG CCTGACACAG AGATTATCAG AGGAAAACCA CATTCCCTTC
    TACCTTCCTG CATCGGCCTC GCTGCGCTTC ATTGCTGAAC TGAAGGGGAA GGCGCTGGTG
    GAGCTGTTCC GTAGCTGCGT TGCCGAGGCA GAGGGGGAGG AGCTGAGGCA TCTCACGTGG
    GCATCACTGC TGCTTTATGG GACCCCCGAT ACAGAGTTTC CATCCTGCAT TTTACTTTCA
    ACACAAGCTG TTTATTTTCT CTTGGATGAT ACTCAGAACC CCAAAGAAGA AGATTATTGG
    AGGAAGAGAC CAGCGCGCAG CCCCCTCCAG ATTTCCTTCT GTTTCGCCAT CAAATTCAGC
    AGTTTACAGT CCGTCAATAT CGGACTTTTT GATCAGTATT TCAGGATAAC AGGTTCTTCC
    CCCAGTCAGG TGGTCACGTG TCTGACCAGG GACAGTTACA GCACCCACTC CTTCATCCAG
    CAGCTCATGT CTGCCCTGTC CCTCCTGGCA CGGGCCCCGT CCCCCGAACC CCTGGAGAAG
    GATTTCTACT CGGAATTTGG TACAAAGAAC ACGGGGAGAA TGGAAAACTA CGAACTGATC
    CATTCCAGCA GAGTGAAATT CGTTTACCCA AACGAGGAGG AGATGGGAGA CCTGACGTTC
    ATAGTGTCGG AGAAGATGGA CCCTTCCAGC CCCATCCAGT CGCTAAACAT TCTGCTGTAC
    GTATTGGGCT TCCGGGTCTC TTGTGTGGGC AGAACCGACC CGCCGCCCTC CCTGCAGCCC
    AACAGCCTCA TCCTCACCAG CTCCGACCTG TTTCTGTTCA ATGAGGATTA TATCAGTTAC
    CCGCTGCCAG ACTTTGCCAA GGAACCCCCC AAGAAGGACA AATACCAGTT AGTTGATGGG
    AGGAGAATCA GGGATTTGGA CCGGGTGCTG ATGGGATACC AGACCTACCC TCAGAACCTC
    ACCTTGGTGT TTGATGATGT TCACAAGCAG GATTTCATGC ACAGCGTCTC TCTCGATCAC
    TTTGGGGAGA CGCTGGGGGG CTCTCCCGGG GCTGTGGGAG CCAATAAAGA GATCCAGTGG
    AACATTTTTA TCCCAAGCGC AGACTCCCGG GAGAAGCTGA TCTCTCTGCT CGCCCGCCAA
    TGGGAGAGCT TGTGCGGCAG GGAGCTTCCC ATCGAACTCA CCGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031755762.1
    CDS79..3666
    Translation

    Target ORF information:

    RefSeq Version XM_031899902.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis nischarin (nisch), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899902.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAAATCCA TGGAATCGCT GCAGTTCTGG CCGAGGAGCT TTTCCATAAA GATCAGCGGC 
    CGGACCGGTC CTGTTGGGAC GAGTAATATC CATGAGCAGT CTCTTCCCTG TGACCTCTCC
    ATCTTCAAAT CCCTCTGTCA GATTGAGGTC AGTCAGTGCA ACGCCCGGCT CCTCAGCGGC
    CTCACTTCCT GTAGGAGGAG TTTAGCCACC ATCAGCATCC ACTGCTCCGC CTCTTCCATG
    AAGGACATTC TGGTGCCGGA GGCCTATGAC TTTGAGCAGT GGGAGCCGGA GGGAGTGGCC
    CCTGGGAGCC CCATTACTGC AGTTGTGCCC AAGTGGAAAG TGCTCACAAC TCTGGATCTG
    AGCCACAATC GGATCTCCTG TATCGATGAG TCTGTTAAAC TGATTCCTGA GATTGAATTC
    TTGGATTTCA GTCACAACGA CATCTCTACA ATAGAAAATC TGCAGCACCT GTACAACCTC
    ATTCACTTGG ACCTGTCCTA CAACAAACTG GCAGACCTGA CTGGCATCTA CACTAAGGTT
    GGGAATATCA AGACTCTCAG TCTGGCCGGG AACGTACTGG AAAGTCTCCG CGGCCTCAAT
    AAATTGTATT CACTTGTTAA CCTGGATCTG AGCCAGAACA GAATTGAGCA GTTGGAAGAA
    GTAAGGAACA TCGGGGGGCT GCCGTGCCTG GAGGGGGTGC TCCTCGCTGG GAACCCTCTC
    ACTGTCATTC CGGATTACAG GACCAAAGTT CTGGCGCTGT TTGGGGACAG AGCGTCTGAG
    GTCTGTTTGG ACAGTACCAG AACCACAGAG AAAGAGCTGG ACACGGTGGA AGTGCTGAAG
    GCCATACAGA AATCAAGGGA CGCACGGAAT AAATTGGCTT CTTCTGAGAA AAAGATCAAT
    GAGGAATTGC GACCCTCTGC TGCTGGCTCT TCCTCCCCTG CCCGTGCTTC CTCTTCCTCT
    CTCTCTCGGC CTATTAGCTG CAGCCAAGGT GCAGATTCTA TAGACCTTTC TTTAGCGAAA
    AGACTGTCCC CAGGTGGAGC CCAACAGAGT GGCTCTGGGG ACCCGGCGGC CAAGCAAGAA
    GCGGAGAGAG GGAAGCCGTG TGAGGGGGAT GTACAGAGAG ACGTGTCTGA CCCTCCCCCG
    GGCAGTGGGA CCAGTTGCCG GGAAGATGCC GTTACGTTGG ACCAATGGGG CCCCTGTCCC
    TGTATGTCGC TGTATCAGGG GGACAAGGAG CTGCTCCCCT ACATTTCTGT GCTGATGGAG
    GGCGCATTGA GGGAACCCCG ACATGTCGCT GGGGAGGCGG AGAATAAGTG GGGGCCCCAC
    AGTTGCGAGT TCCATAAGAC AGAAGACGGT TACTTTGAGA TGGGGCCGGA TACCCAAACG
    ACAGATGGAA CCTTCCCAGC TGCCCCGGCT GTCAGTCACA TAGTGTGGGG TCTCTCGTTC
    CACAGTCGGG GGGCAGCGGA GCTCAGACCC TTCGCCTCGT GCCTTGTGCT CACCGATACC
    CTCGTCGTCT GCTTTGAGCT GGCGCCCGAC TCCGCCCCCA ACTTACTGCG ACTGGTTCTG
    TGTTTCGCCT ATGGGGACGT CGCAGGATTT CATTTCCCCG TCCCGGACAT TTGCCTGAGA
    GTCAGTTTGG GGGACGGGGA GGAGAGTGTC TTTGTTATGT CAGACTTGCA GGGCTTGCAG
    CAACTCTCTG CCTGTTTGAA AGCCCGCTGC CCCCCAAGCC ACACCCCTGA GAGGATTATT
    CCTGAACCAA TGAGCTTGCA GGATTTGCTC CTTTATATTT CCGAGGTGCA GCAAGTCGTA
    CTGAGCGATA GCGACGTAAA GGGATGTTTC CCGGTGTATC TGTTTAACGG TGAGGCCGCC
    GGTGACCCCG CCCAACCAGA GCCTCCATTT CATACACAAA CATGGCCTCC AGCTGCCTCC
    TGTGCTGCAC TCCTTTCCAT CATATCTACA GGCCTTCAGC CCGGGGGGGA AAGGCTGCTC
    CCCCATTGGC TACTCCTCAC TCAGCAATGT TTCATACTAA TTACAGTCGA CATTAGTACA
    ATAACCGGTA ACCGGGCGGA GTACGCGGGA AATGCGTTAA CGATGAGCGC CATTCCCTTG
    GCTTCGGTTG CGTTGGAGCC GGAGAGTCCC CCTGCTGAGA ATAAGCCCCC CCAGTTTGCC
    GGACACACTG TCCCTTTAAT GGTGGATTAC AGAGCAGTCA GCGCATTCTT TGTTGTGCCC
    CAAAATAAAT TCCACTTCTT GTCGGTGTTT AACCAGCTGA GAAAGTCCCT TCAGGGGATA
    AAGAACTTGG TGATTTGCAA ATCCATAAAA AATCTGGACG ATTCCCAGAG CTGGGTGGGC
    GACGCCCACA GACCCCCAAC GCACTCGCTA AGCCCCGTTG CGCCCCACAG GGCACGGCCC
    CCCCAGTACC CATCAGAGAG CCTGACACAG AGATTATCAG AGGAAAACCA CATTCCCTTC
    TACCTTCCTG CATCGGCCTC GCTGCGCTTC ATTGCTGAAC TGAAGGGGAA GGCGCTGGTG
    GAGCTGTTCC GTAGCTGCGT TGCCGAGGCA GAGGGGGAGG AGCTGAGGCA TCTCACGTGG
    GCATCACTGC TGCTTTATGG GACCCCCGAT ACAGAGTTTC CATCCTGCAT TTTACTTTCA
    ACACAAGCTG TTTATTTTCT CTTGGATGAT ACTCAGAACC CCAAAGAAGA AGATTATTGG
    AGGAAGAGAC CAGCGCGCAG CCCCCTCCAG ATTTCCTTCT GTTTCGCCAT CAAATTCAGC
    AGTTTACAGT CCGTCAATAT CGGACTTTTT GATCAGTATT TCAGGATAAC AGGTTCTTCC
    CCCAGTCAGG TGGTCACGTG TCTGACCAGG GACAGTTACA GCACCCACTC CTTCATCCAG
    CAGCTCATGT CTGCCCTGTC CCTCCTGGCA CGGGCCCCGT CCCCCGAACC CCTGGAGAAG
    GATTTCTACT CGGAATTTGG TACAAAGAAC ACGGGGAGAA TGGAAAACTA CGAACTGATC
    CATTCCAGCA GAGTGAAATT CGTTTACCCA AACGAGGAGG AGATGGGAGA CCTGACGTTC
    ATAGTGTCGG AGAAGATGGA CCCTTCCAGC CCCATCCAGT CGCTAAACAT TCTGCTGTAC
    GTATTGGGCT TCCGGGTCTC TTGTGTGGGC AGAACCGACC CGCCGCCCTC CCTGCAGCCC
    AACAGCCTCA TCCTCACCAG CTCCGACCTG TTTCTGTTCA ATGAGGATTA TATCAGTTAC
    CCGCTGCCAG ACTTTGCCAA GGAACCCCCC AAGAAGGACA AATACCAGTT AGTTGATGGG
    AGGAGAATCA GGGATTTGGA CCGGGTGCTG ATGGGATACC AGACCTACCC TCAGAACCTC
    ACCTTGGTGT TTGATGATGT TCACAAGCAG GATTTCATGC ACAGCGTCTC TCTCGATCAC
    TTTGGGGAGA CGCTGGGGGG CTCTCCCGGG GCTGTGGGAG CCAATAAAGA GATCCAGTGG
    AACATTTTTA TCCCAAGCGC AGACTCCCGG GAGAAGCTGA TCTCTCTGCT CGCCCGCCAA
    TGGGAGAGCT TGTGCGGCAG GGAGCTTCCC ATCGAACTCA CCGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55828
    Clone ID Related Accession (Same CDS sequence) XM_031899903.1
    Accession Version XM_031899903.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3579bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nischarin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGAATCGC TGCAGTTCTG GCCGAGGAGC TTTTCCATAA AGATCAGCGG CCGGACCGGT 
    CCTGTTGGGA CGAGTAATAT CCATGAGCAG TCTCTTCCCT GTGACCTCTC CATCTTCAAA
    TCCCTCTGTC AGATTGAGGT CAGTCAGTGC AACGCCCGGC TCCTCAGCGG CCTCACTTCC
    TGTAGGAGGA GTTTAGCCAC CATCAGCATC CACTGCTCCG CCTCTTCCAT GAAGGACATT
    CTGGTGCCGG AGGCCTATGA CTTTGAGCAG TGGGAGCCGG AGGGAGTGGC CCCTGGGAGC
    CCCATTACTG CAGTTGTGCC CAAGTGGAAA GTGCTCACAA CTCTGGATCT GAGCCACAAT
    CGGATCTCCT GTATCGATGA GTCTGTTAAA CTGATTCCTG AGATTGAATT CTTGGATTTC
    AGTCACAACG ACATCTCTAC AATAGAAAAT CTGCAGCACC TGTACAACCT CATTCACTTG
    GACCTGTCCT ACAACAAACT GGCAGACCTG ACTGGCATCT ACACTAAGGT TGGGAATATC
    AAGACTCTCA GTCTGGCCGG GAACGTACTG GAAAGTCTCC GCGGCCTCAA TAAATTGTAT
    TCACTTGTTA ACCTGGATCT GAGCCAGAAC AGAATTGAGC AGTTGGAAGA AGTAAGGAAC
    ATCGGGGGGC TGCCGTGCCT GGAGGGGGTG CTCCTCGCTG GGAACCCTCT CACTGTCATT
    CCGGATTACA GGACCAAAGT TCTGGCGCTG TTTGGGGACA GAGCGTCTGA GGTCTGTTTG
    GACAGTACCA GAACCACAGA GAAAGAGCTG GACACGGTGG AAGTGCTGAA GGCCATACAG
    AAATCAAGGG ACGCACGGAA TAAATTGGCT TCTTCTGAGA AAAAGATCAA TGAGGAATTG
    CGACCCTCTG CTGCTGGCTC TTCCTCCCCT GCCCGTGCTT CCTCTTCCTC TCTCTCTCGG
    CCTATTAGCT GCAGCCAAGG TGCAGATTCT ATAGACCTTT CTTTAGCGAA AAGACTGTCC
    CCAGGTGGAG CCCAACAGAG TGGCTCTGGG GACCCGGCGG CCAAGCAAGA AGCGGAGAGA
    GGGAAGCCGT GTGAGGGGGA TGTACAGAGA GACGTGTCTG ACCCTCCCCC GGGCAGTGGG
    ACCAGTTGCC GGGAAGATGC CGTTACGTTG GACCAATGGG GCCCCTGTCC CTGTATGTCG
    CTGTATCAGG GGGACAAGGA GCTGCTCCCC TACATTTCTG TGCTGATGGA GGGCGCATTG
    AGGGAACCCC GACATGTCGC TGGGGAGGCG GAGAATAAGT GGGGGCCCCA CAGTTGCGAG
    TTCCATAAGA CAGAAGACGG TTACTTTGAG ATGGGGCCGG ATACCCAAAC GACAGATGGA
    ACCTTCCCAG CTGCCCCGGC TGTCAGTCAC ATAGTGTGGG GTCTCTCGTT CCACAGTCGG
    GGGGCAGCGG AGCTCAGACC CTTCGCCTCG TGCCTTGTGC TCACCGATAC CCTCGTCGTC
    TGCTTTGAGC TGGCGCCCGA CTCCGCCCCC AACTTACTGC GACTGGTTCT GTGTTTCGCC
    TATGGGGACG TCGCAGGATT TCATTTCCCC GTCCCGGACA TTTGCCTGAG AGTCAGTTTG
    GGGGACGGGG AGGAGAGTGT CTTTGTTATG TCAGACTTGC AGGGCTTGCA GCAACTCTCT
    GCCTGTTTGA AAGCCCGCTG CCCCCCAAGC CACACCCCTG AGAGGATTAT TCCTGAACCA
    ATGAGCTTGC AGGATTTGCT CCTTTATATT TCCGAGGTGC AGCAAGTCGT ACTGAGCGAT
    AGCGACGTAA AGGGATGTTT CCCGGTGTAT CTGTTTAACG GTGAGGCCGC CGGTGACCCC
    GCCCAACCAG AGCCTCCATT TCATACACAA ACATGGCCTC CAGCTGCCTC CTGTGCTGCA
    CTCCTTTCCA TCATATCTAC AGGCCTTCAG CCCGGGGGGG AAAGGCTGCT CCCCCATTGG
    CTACTCCTCA CTCAGCAATG TTTCATACTA ATTACAGTCG ACATTAGTAC AATAACCGGT
    AACCGGGCGG AGTACGCGGG AAATGCGTTA ACGATGAGCG CCATTCCCTT GGCTTCGGTT
    GCGTTGGAGC CGGAGAGTCC CCCTGCTGAG AATAAGCCCC CCCAGTTTGC CGGACACACT
    GTCCCTTTAA TGGTGGATTA CAGAGCAGTC AGCGCATTCT TTGTTGTGCC CCAAAATAAA
    TTCCACTTCT TGTCGGTGTT TAACCAGCTG AGAAAGTCCC TTCAGGGGAT AAAGAACTTG
    GTGATTTGCA AATCCATAAA AAATCTGGAC GATTCCCAGA GCTGGGTGGG CGACGCCCAC
    AGACCCCCAA CGCACTCGCT AAGCCCCGTT GCGCCCCACA GGGCACGGCC CCCCCAGTAC
    CCATCAGAGA GCCTGACACA GAGATTATCA GAGGAAAACC ACATTCCCTT CTACCTTCCT
    GCATCGGCCT CGCTGCGCTT CATTGCTGAA CTGAAGGGGA AGGCGCTGGT GGAGCTGTTC
    CGTAGCTGCG TTGCCGAGGC AGAGGGGGAG GAGCTGAGGC ATCTCACGTG GGCATCACTG
    CTGCTTTATG GGACCCCCGA TACAGAGTTT CCATCCTGCA TTTTACTTTC AACACAAGCT
    GTTTATTTTC TCTTGGATGA TACTCAGAAC CCCAAAGAAG AAGATTATTG GAGGAAGAGA
    CCAGCGCGCA GCCCCCTCCA GATTTCCTTC TGTTTCGCCA TCAAATTCAG CAGTTTACAG
    TCCGTCAATA TCGGACTTTT TGATCAGTAT TTCAGGATAA CAGGTTCTTC CCCCAGTCAG
    GTGGTCACGT GTCTGACCAG GGACAGTTAC AGCACCCACT CCTTCATCCA GCAGCTCATG
    TCTGCCCTGT CCCTCCTGGC ACGGGCCCCG TCCCCCGAAC CCCTGGAGAA GGATTTCTAC
    TCGGAATTTG GTACAAAGAA CACGGGGAGA ATGGAAAACT ACGAACTGAT CCATTCCAGC
    AGAGTGAAAT TCGTTTACCC AAACGAGGAG GAGATGGGAG ACCTGACGTT CATAGTGTCG
    GAGAAGATGG ACCCTTCCAG CCCCATCCAG TCGCTAAACA TTCTGCTGTA CGTATTGGGC
    TTCCGGGTCT CTTGTGTGGG CAGAACCGAC CCGCCGCCCT CCCTGCAGCC CAACAGCCTC
    ATCCTCACCA GCTCCGACCT GTTTCTGTTC AATGAGGATT ATATCAGTTA CCCGCTGCCA
    GACTTTGCCA AGGAACCCCC CAAGAAGGAC AAATACCAGT TAGTTGATGG GAGGAGAATC
    AGGGATTTGG ACCGGGTGCT GATGGGATAC CAGACCTACC CTCAGAACCT CACCTTGGTG
    TTTGATGATG TTCACAAGCA GGATTTCATG CACAGCGTCT CTCTCGATCA CTTTGGGGAG
    ACGCTGGGGG GCTCTCCCGG GGCTGTGGGA GCCAATAAAG AGATCCAGTG GAACATTTTT
    ATCCCAAGCG CAGACTCCCG GGAGAAGCTG ATCTCTCTGC TCGCCCGCCA ATGGGAGAGC
    TTGTGCGGCA GGGAGCTTCC CATCGAACTC ACCGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031755763.1
    CDS648..4226
    Translation

    Target ORF information:

    RefSeq Version XM_031899903.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis nischarin (nisch), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899903.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGAATCGC TGCAGTTCTG GCCGAGGAGC TTTTCCATAA AGATCAGCGG CCGGACCGGT 
    CCTGTTGGGA CGAGTAATAT CCATGAGCAG TCTCTTCCCT GTGACCTCTC CATCTTCAAA
    TCCCTCTGTC AGATTGAGGT CAGTCAGTGC AACGCCCGGC TCCTCAGCGG CCTCACTTCC
    TGTAGGAGGA GTTTAGCCAC CATCAGCATC CACTGCTCCG CCTCTTCCAT GAAGGACATT
    CTGGTGCCGG AGGCCTATGA CTTTGAGCAG TGGGAGCCGG AGGGAGTGGC CCCTGGGAGC
    CCCATTACTG CAGTTGTGCC CAAGTGGAAA GTGCTCACAA CTCTGGATCT GAGCCACAAT
    CGGATCTCCT GTATCGATGA GTCTGTTAAA CTGATTCCTG AGATTGAATT CTTGGATTTC
    AGTCACAACG ACATCTCTAC AATAGAAAAT CTGCAGCACC TGTACAACCT CATTCACTTG
    GACCTGTCCT ACAACAAACT GGCAGACCTG ACTGGCATCT ACACTAAGGT TGGGAATATC
    AAGACTCTCA GTCTGGCCGG GAACGTACTG GAAAGTCTCC GCGGCCTCAA TAAATTGTAT
    TCACTTGTTA ACCTGGATCT GAGCCAGAAC AGAATTGAGC AGTTGGAAGA AGTAAGGAAC
    ATCGGGGGGC TGCCGTGCCT GGAGGGGGTG CTCCTCGCTG GGAACCCTCT CACTGTCATT
    CCGGATTACA GGACCAAAGT TCTGGCGCTG TTTGGGGACA GAGCGTCTGA GGTCTGTTTG
    GACAGTACCA GAACCACAGA GAAAGAGCTG GACACGGTGG AAGTGCTGAA GGCCATACAG
    AAATCAAGGG ACGCACGGAA TAAATTGGCT TCTTCTGAGA AAAAGATCAA TGAGGAATTG
    CGACCCTCTG CTGCTGGCTC TTCCTCCCCT GCCCGTGCTT CCTCTTCCTC TCTCTCTCGG
    CCTATTAGCT GCAGCCAAGG TGCAGATTCT ATAGACCTTT CTTTAGCGAA AAGACTGTCC
    CCAGGTGGAG CCCAACAGAG TGGCTCTGGG GACCCGGCGG CCAAGCAAGA AGCGGAGAGA
    GGGAAGCCGT GTGAGGGGGA TGTACAGAGA GACGTGTCTG ACCCTCCCCC GGGCAGTGGG
    ACCAGTTGCC GGGAAGATGC CGTTACGTTG GACCAATGGG GCCCCTGTCC CTGTATGTCG
    CTGTATCAGG GGGACAAGGA GCTGCTCCCC TACATTTCTG TGCTGATGGA GGGCGCATTG
    AGGGAACCCC GACATGTCGC TGGGGAGGCG GAGAATAAGT GGGGGCCCCA CAGTTGCGAG
    TTCCATAAGA CAGAAGACGG TTACTTTGAG ATGGGGCCGG ATACCCAAAC GACAGATGGA
    ACCTTCCCAG CTGCCCCGGC TGTCAGTCAC ATAGTGTGGG GTCTCTCGTT CCACAGTCGG
    GGGGCAGCGG AGCTCAGACC CTTCGCCTCG TGCCTTGTGC TCACCGATAC CCTCGTCGTC
    TGCTTTGAGC TGGCGCCCGA CTCCGCCCCC AACTTACTGC GACTGGTTCT GTGTTTCGCC
    TATGGGGACG TCGCAGGATT TCATTTCCCC GTCCCGGACA TTTGCCTGAG AGTCAGTTTG
    GGGGACGGGG AGGAGAGTGT CTTTGTTATG TCAGACTTGC AGGGCTTGCA GCAACTCTCT
    GCCTGTTTGA AAGCCCGCTG CCCCCCAAGC CACACCCCTG AGAGGATTAT TCCTGAACCA
    ATGAGCTTGC AGGATTTGCT CCTTTATATT TCCGAGGTGC AGCAAGTCGT ACTGAGCGAT
    AGCGACGTAA AGGGATGTTT CCCGGTGTAT CTGTTTAACG GTGAGGCCGC CGGTGACCCC
    GCCCAACCAG AGCCTCCATT TCATACACAA ACATGGCCTC CAGCTGCCTC CTGTGCTGCA
    CTCCTTTCCA TCATATCTAC AGGCCTTCAG CCCGGGGGGG AAAGGCTGCT CCCCCATTGG
    CTACTCCTCA CTCAGCAATG TTTCATACTA ATTACAGTCG ACATTAGTAC AATAACCGGT
    AACCGGGCGG AGTACGCGGG AAATGCGTTA ACGATGAGCG CCATTCCCTT GGCTTCGGTT
    GCGTTGGAGC CGGAGAGTCC CCCTGCTGAG AATAAGCCCC CCCAGTTTGC CGGACACACT
    GTCCCTTTAA TGGTGGATTA CAGAGCAGTC AGCGCATTCT TTGTTGTGCC CCAAAATAAA
    TTCCACTTCT TGTCGGTGTT TAACCAGCTG AGAAAGTCCC TTCAGGGGAT AAAGAACTTG
    GTGATTTGCA AATCCATAAA AAATCTGGAC GATTCCCAGA GCTGGGTGGG CGACGCCCAC
    AGACCCCCAA CGCACTCGCT AAGCCCCGTT GCGCCCCACA GGGCACGGCC CCCCCAGTAC
    CCATCAGAGA GCCTGACACA GAGATTATCA GAGGAAAACC ACATTCCCTT CTACCTTCCT
    GCATCGGCCT CGCTGCGCTT CATTGCTGAA CTGAAGGGGA AGGCGCTGGT GGAGCTGTTC
    CGTAGCTGCG TTGCCGAGGC AGAGGGGGAG GAGCTGAGGC ATCTCACGTG GGCATCACTG
    CTGCTTTATG GGACCCCCGA TACAGAGTTT CCATCCTGCA TTTTACTTTC AACACAAGCT
    GTTTATTTTC TCTTGGATGA TACTCAGAAC CCCAAAGAAG AAGATTATTG GAGGAAGAGA
    CCAGCGCGCA GCCCCCTCCA GATTTCCTTC TGTTTCGCCA TCAAATTCAG CAGTTTACAG
    TCCGTCAATA TCGGACTTTT TGATCAGTAT TTCAGGATAA CAGGTTCTTC CCCCAGTCAG
    GTGGTCACGT GTCTGACCAG GGACAGTTAC AGCACCCACT CCTTCATCCA GCAGCTCATG
    TCTGCCCTGT CCCTCCTGGC ACGGGCCCCG TCCCCCGAAC CCCTGGAGAA GGATTTCTAC
    TCGGAATTTG GTACAAAGAA CACGGGGAGA ATGGAAAACT ACGAACTGAT CCATTCCAGC
    AGAGTGAAAT TCGTTTACCC AAACGAGGAG GAGATGGGAG ACCTGACGTT CATAGTGTCG
    GAGAAGATGG ACCCTTCCAG CCCCATCCAG TCGCTAAACA TTCTGCTGTA CGTATTGGGC
    TTCCGGGTCT CTTGTGTGGG CAGAACCGAC CCGCCGCCCT CCCTGCAGCC CAACAGCCTC
    ATCCTCACCA GCTCCGACCT GTTTCTGTTC AATGAGGATT ATATCAGTTA CCCGCTGCCA
    GACTTTGCCA AGGAACCCCC CAAGAAGGAC AAATACCAGT TAGTTGATGG GAGGAGAATC
    AGGGATTTGG ACCGGGTGCT GATGGGATAC CAGACCTACC CTCAGAACCT CACCTTGGTG
    TTTGATGATG TTCACAAGCA GGATTTCATG CACAGCGTCT CTCTCGATCA CTTTGGGGAG
    ACGCTGGGGG GCTCTCCCGG GGCTGTGGGA GCCAATAAAG AGATCCAGTG GAACATTTTT
    ATCCCAAGCG CAGACTCCCG GGAGAAGCTG ATCTCTCTGC TCGCCCGCCA ATGGGAGAGC
    TTGTGCGGCA GGGAGCTTCC CATCGAACTC ACCGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55829
    Clone ID Related Accession (Same CDS sequence) XM_031899904.1
    Accession Version XM_031899904.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3369bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nischarin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGAGGACG AGGAGGCAGA GGAACGACGG GAGGTCCGGG TTGTGGGCTC CGAACTGGTG 
    GAGAACTACA CGGTTTATAT CATTCAGCTC CGCGCTGGGA GTTTCCACTG GGAAGTGAGA
    CACCGATACA GCGACTTCTA TGATCTCCAT GAGAAGTTGA CGGCGGAGAA TAAAATCGAG
    AGGAACCTTT TGCCCCCGAA GAAGATGATC GGGAAGAACT CGAGAAGTTT GGTGGAAAAG
    CGGCAGAAAG ATCTGGAATT GTATCTGCAG ACGCTGCTTG GCACGTTCCC CCTCGCTGTG
    CCCAAAGCCC TGGCACGCTT CCTGCATTTC CACCTCTATG AAATCCATGG AATCGCTGCA
    GTTCTGGCCG AGGAGCTTTT CCATAAAGGG GAGCAGTTGC TATTGGCCGG AGAGGTATTT
    ACCGTGACCC CACTGCAGCT CTATGCCGTG ACGCAGTTAC TCCGACGCGC CAAGCCCACC
    TGTAGCACCG GCGACGCCAG GAACGACCTG GGGCACATCA TGGATTTTGC TTGTAGACTG
    AAATACCTCC GGATCAGCGG CCGGACCGGT CCTGTTGGGA CGAGTAATAT CCATGAGCAG
    TCTCTTCCCT GTGACCTCTC CATCTTCAAA TCCCTCTGTC AGATTGAGGT CAGTCAGTGC
    AACGCCCGGC TCCTCAGCGG CCTCACTTCC TGTAGGAGGA GTTTAGCCAC CATCAGCATC
    CACTGCTCCG CCTCTTCCAT GAAGGACATT CTGGTGCCGG AGGCCTATGA CTTTGAGCAG
    TGGGAGCCGG AGGGAGTGGC CCCTGGGAGC CCCATTACTG CAGTTGTGCC CAAGTGGAAA
    GTGCTCACAA CTCTGGATCT GAGCCACAAT CGGATCTCCT GTATCGATGA GTCTGTTAAA
    CTGATTCCTG AGATTGAATT CTTGGATTTC AGTCACAACG ACATCTCTAC AATAGAAAAT
    CTGCAGCACC TGTACAACCT CATTCACTTG GACCTGTCCT ACAACAAACT GGCAGACCTG
    ACTGGCATCT ACACTAAGGT TGGGAATATC AAGACTCTCA GTCTGGCCGG GAACGTACTG
    GAAAGTCTCC GCGGCCTCAA TAAATTGTAT TCACTTGTTA ACCTGGATCT GAGCCAGAAC
    AGAATTGAGC AGTTGGAAGA AGTAAGGAAC ATCGGGGGGC TGCCGTGCCT GGAGGGGGTG
    CTCCTCGCTG GGAACCCTCT CACTGTCATT CCGGATTACA GGACCAAAGT TCTGGCGCTG
    TTTGGGGACA GAGCGTCTGA GGTCTGTTTG GACAGTACCA GAACCACAGA GAAAGAGCTG
    GACACGGTGG AAGTGCTGAA GGCCATACAG AAATCAAGGG ACGCACGGAA TAAATTGGCT
    TCTTCTGAGA AAAAGATCAA TGAGGAATTG CGACCCTCTG CTGCTGGCTC TTCCTCCCCT
    GCCCGTGCTT CCTCTTCCTC TCTCTCTCGG CCTATTAGCT GCAGCCAAGG TGCAGATTCT
    ATAGACCTTT CTTTAGCGAA AAGACTGTCC CCAGGTGGAG CCCAACAGAG TGGCTCTGGG
    GACCCGGCGG CCAAGCAAGA AGCGGAGAGA GGGAAGCCGT GTGAGGGGGA TGTACAGAGA
    GACGTGTCTG ACCCTCCCCC GGGCAGTGGG ACCAGTTGCC GGGAAGATGC CGTTACGTTG
    GACCAATGGG GCCCCTGTCC CTGTATGTCG CTGTATCAGG GGGACAAGGA GCTGCTCCCC
    TACATTTCTG TGCTGATGGA GGGCGCATTG AGGGAACCCC GACATGTCGC TGGGGAGGCG
    GAGAATAAGT GGGGGCCCCA CAGTTGCGAG TTCCATAAGA CAGAAGACGG TTACTTTGAG
    ATGGGGCCGG ATACCCAAAC GACAGATGGA ACCTTCCCAG CTGCCCCGGC TGTCAGTCAC
    ATAGTGTGGG GTCTCTCGTT CCACAGTCGG GGGGCAGCGG AGCTCAGACC CTTCGCCTCG
    TGCCTTGTGC TCACCGATAC CCTCGTCGTC TGCTTTGAGC TGGCGCCCGA CTCCGCCCCC
    AACTTACTGC GACTGGTTCT GTGTTTCGCC TATGGGGACG TCGCAGGATT TCATTTCCCC
    GTCCCGGACA TTTGCCTGAG AGTCAGTTTG GGGGACGGGG AGGAGAGTGT CTTTGTTATG
    TCAGACTTGC AGGGCTTGCA GCAACTCTCT GCCTGTTTGA AAGCCCGCTG CCCCCCAAGC
    CACACCCCTG AGAGGATTAT TCCTGAACCA ATGAGCTTGC AGGATTTGCT CCTTTATATT
    TCCGAGGTGC AGCAAGTCGT ACTGAGCGAT AGCGACGTAA AGGGATGTTT CCCGGTGTAT
    CTGTTTAACG GTGAGGCCGC CGGTGACCCC GCCCAACCAG AGCCTCCATT TCATACACAA
    ACATGGCCTC CAGCTGCCTC CTGTGCTGCA CTCCTTTCCA TCATATCTAC AGGCCTTCAG
    CCCGGGGGGG AAAGGCTGCT CCCCCATTGG CTACTCCTCA CTCAGCAATG TTTCATACTA
    ATTACAGTCG ACATTAGTAC AATAACCGGT AACCGGGCGG AGTACGCGGG AAATGCGTTA
    ACGATGAGCG CCATTCCCTT GGCTTCGGTT GCGTTGGAGC CGGAGAGTCC CCCTGCTGAG
    AATAAGCCCC CCCAGTTTGC CGGACACACT GTCCCTTTAA TGGTGGATTA CAGAGCAGTC
    AGCGCATTCT TTGTTGTGCC CCAAAATAAA TTCCACTTCT TGTCGGTGTT TAACCAGCTG
    AGAAAGTCCC TTCAGGGGAT AAAGAACTTG GTGATTTGCA AATCCATAAA AAATCTGGAC
    GATTCCCAGA GCTGGGTGGG CGACGCCCAC AGACCCCCAA CGCACTCGCT AAGCCCCGTT
    GCGCCCCACA GGGCACGGCC CCCCCAGTAC CCATCAGAGA GCCTGACACA GAGATTATCA
    GAGGAAAACC ACATTCCCTT CTACCTTCCT GCATCGGCCT CGCTGCGCTT CATTGCTGAA
    CTGAAGGGGA AGGCGCTGGT GGAGCTGTTC CGTAGCTGCG TTGCCGAGGC AGAGGGGGAG
    GAGCTGAGGC ATCTCACGTG GGCATCACTG CTGCTTTATG GGACCCCCGA TACAGAGTTT
    CCATCCTGCA TTTTACTTTC AACACAAGCT GTTTATTTTC TCTTGGATGA TACTCAGAAC
    CCCAAAGAAG AAGTAAACTG GGCAGAAAAC TGTCAGAATA GTCAGAAAAC TGGCGGAATA
    GTCGGAATGG GTAGAAAACT GTCGGAATGG GCAGAAAACT GTCAGAATGG GCAGAAAACT
    GTCGGAATGG GCAGAAAACT GTCGGAATAC TCACAAGACT CACAAGAATT GGCAGAAAAC
    TCAGAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031755764.1
    CDS566..3934
    Translation

    Target ORF information:

    RefSeq Version XM_031899904.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis nischarin (nisch), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899904.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGAGGACG AGGAGGCAGA GGAACGACGG GAGGTCCGGG TTGTGGGCTC CGAACTGGTG 
    GAGAACTACA CGGTTTATAT CATTCAGCTC CGCGCTGGGA GTTTCCACTG GGAAGTGAGA
    CACCGATACA GCGACTTCTA TGATCTCCAT GAGAAGTTGA CGGCGGAGAA TAAAATCGAG
    AGGAACCTTT TGCCCCCGAA GAAGATGATC GGGAAGAACT CGAGAAGTTT GGTGGAAAAG
    CGGCAGAAAG ATCTGGAATT GTATCTGCAG ACGCTGCTTG GCACGTTCCC CCTCGCTGTG
    CCCAAAGCCC TGGCACGCTT CCTGCATTTC CACCTCTATG AAATCCATGG AATCGCTGCA
    GTTCTGGCCG AGGAGCTTTT CCATAAAGGG GAGCAGTTGC TATTGGCCGG AGAGGTATTT
    ACCGTGACCC CACTGCAGCT CTATGCCGTG ACGCAGTTAC TCCGACGCGC CAAGCCCACC
    TGTAGCACCG GCGACGCCAG GAACGACCTG GGGCACATCA TGGATTTTGC TTGTAGACTG
    AAATACCTCC GGATCAGCGG CCGGACCGGT CCTGTTGGGA CGAGTAATAT CCATGAGCAG
    TCTCTTCCCT GTGACCTCTC CATCTTCAAA TCCCTCTGTC AGATTGAGGT CAGTCAGTGC
    AACGCCCGGC TCCTCAGCGG CCTCACTTCC TGTAGGAGGA GTTTAGCCAC CATCAGCATC
    CACTGCTCCG CCTCTTCCAT GAAGGACATT CTGGTGCCGG AGGCCTATGA CTTTGAGCAG
    TGGGAGCCGG AGGGAGTGGC CCCTGGGAGC CCCATTACTG CAGTTGTGCC CAAGTGGAAA
    GTGCTCACAA CTCTGGATCT GAGCCACAAT CGGATCTCCT GTATCGATGA GTCTGTTAAA
    CTGATTCCTG AGATTGAATT CTTGGATTTC AGTCACAACG ACATCTCTAC AATAGAAAAT
    CTGCAGCACC TGTACAACCT CATTCACTTG GACCTGTCCT ACAACAAACT GGCAGACCTG
    ACTGGCATCT ACACTAAGGT TGGGAATATC AAGACTCTCA GTCTGGCCGG GAACGTACTG
    GAAAGTCTCC GCGGCCTCAA TAAATTGTAT TCACTTGTTA ACCTGGATCT GAGCCAGAAC
    AGAATTGAGC AGTTGGAAGA AGTAAGGAAC ATCGGGGGGC TGCCGTGCCT GGAGGGGGTG
    CTCCTCGCTG GGAACCCTCT CACTGTCATT CCGGATTACA GGACCAAAGT TCTGGCGCTG
    TTTGGGGACA GAGCGTCTGA GGTCTGTTTG GACAGTACCA GAACCACAGA GAAAGAGCTG
    GACACGGTGG AAGTGCTGAA GGCCATACAG AAATCAAGGG ACGCACGGAA TAAATTGGCT
    TCTTCTGAGA AAAAGATCAA TGAGGAATTG CGACCCTCTG CTGCTGGCTC TTCCTCCCCT
    GCCCGTGCTT CCTCTTCCTC TCTCTCTCGG CCTATTAGCT GCAGCCAAGG TGCAGATTCT
    ATAGACCTTT CTTTAGCGAA AAGACTGTCC CCAGGTGGAG CCCAACAGAG TGGCTCTGGG
    GACCCGGCGG CCAAGCAAGA AGCGGAGAGA GGGAAGCCGT GTGAGGGGGA TGTACAGAGA
    GACGTGTCTG ACCCTCCCCC GGGCAGTGGG ACCAGTTGCC GGGAAGATGC CGTTACGTTG
    GACCAATGGG GCCCCTGTCC CTGTATGTCG CTGTATCAGG GGGACAAGGA GCTGCTCCCC
    TACATTTCTG TGCTGATGGA GGGCGCATTG AGGGAACCCC GACATGTCGC TGGGGAGGCG
    GAGAATAAGT GGGGGCCCCA CAGTTGCGAG TTCCATAAGA CAGAAGACGG TTACTTTGAG
    ATGGGGCCGG ATACCCAAAC GACAGATGGA ACCTTCCCAG CTGCCCCGGC TGTCAGTCAC
    ATAGTGTGGG GTCTCTCGTT CCACAGTCGG GGGGCAGCGG AGCTCAGACC CTTCGCCTCG
    TGCCTTGTGC TCACCGATAC CCTCGTCGTC TGCTTTGAGC TGGCGCCCGA CTCCGCCCCC
    AACTTACTGC GACTGGTTCT GTGTTTCGCC TATGGGGACG TCGCAGGATT TCATTTCCCC
    GTCCCGGACA TTTGCCTGAG AGTCAGTTTG GGGGACGGGG AGGAGAGTGT CTTTGTTATG
    TCAGACTTGC AGGGCTTGCA GCAACTCTCT GCCTGTTTGA AAGCCCGCTG CCCCCCAAGC
    CACACCCCTG AGAGGATTAT TCCTGAACCA ATGAGCTTGC AGGATTTGCT CCTTTATATT
    TCCGAGGTGC AGCAAGTCGT ACTGAGCGAT AGCGACGTAA AGGGATGTTT CCCGGTGTAT
    CTGTTTAACG GTGAGGCCGC CGGTGACCCC GCCCAACCAG AGCCTCCATT TCATACACAA
    ACATGGCCTC CAGCTGCCTC CTGTGCTGCA CTCCTTTCCA TCATATCTAC AGGCCTTCAG
    CCCGGGGGGG AAAGGCTGCT CCCCCATTGG CTACTCCTCA CTCAGCAATG TTTCATACTA
    ATTACAGTCG ACATTAGTAC AATAACCGGT AACCGGGCGG AGTACGCGGG AAATGCGTTA
    ACGATGAGCG CCATTCCCTT GGCTTCGGTT GCGTTGGAGC CGGAGAGTCC CCCTGCTGAG
    AATAAGCCCC CCCAGTTTGC CGGACACACT GTCCCTTTAA TGGTGGATTA CAGAGCAGTC
    AGCGCATTCT TTGTTGTGCC CCAAAATAAA TTCCACTTCT TGTCGGTGTT TAACCAGCTG
    AGAAAGTCCC TTCAGGGGAT AAAGAACTTG GTGATTTGCA AATCCATAAA AAATCTGGAC
    GATTCCCAGA GCTGGGTGGG CGACGCCCAC AGACCCCCAA CGCACTCGCT AAGCCCCGTT
    GCGCCCCACA GGGCACGGCC CCCCCAGTAC CCATCAGAGA GCCTGACACA GAGATTATCA
    GAGGAAAACC ACATTCCCTT CTACCTTCCT GCATCGGCCT CGCTGCGCTT CATTGCTGAA
    CTGAAGGGGA AGGCGCTGGT GGAGCTGTTC CGTAGCTGCG TTGCCGAGGC AGAGGGGGAG
    GAGCTGAGGC ATCTCACGTG GGCATCACTG CTGCTTTATG GGACCCCCGA TACAGAGTTT
    CCATCCTGCA TTTTACTTTC AACACAAGCT GTTTATTTTC TCTTGGATGA TACTCAGAAC
    CCCAAAGAAG AAGTAAACTG GGCAGAAAAC TGTCAGAATA GTCAGAAAAC TGGCGGAATA
    GTCGGAATGG GTAGAAAACT GTCGGAATGG GCAGAAAACT GTCAGAATGG GCAGAAAACT
    GTCGGAATGG GCAGAAAACT GTCGGAATAC TCACAAGACT CACAAGAATT GGCAGAAAAC
    TCAGAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa23481
    Clone ID Related Accession (Same CDS sequence) NM_001130345.1
    Accession Version NM_001130345.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3885bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nischarin
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC167624.1. ##Evidence-Data-START## Transcript exon combination :: BC167624.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGATCGGGA AGAACTCGAG AAGTTTGGTG GAAAAGCGGC AGAAAGATCT GGAATTGTAT 
    CTGCAGACGC TGCTTGGCAC GTTCCCCCTC GCTGTGCCCA AAGCCCTGGC ACGCTTCCTG
    CATTTCCACC TCTATGAAAT CCATGGAATC GCTGCAGTTC TGGCCGAGGA GCTTTTCCAT
    AAAGGGGAGC AGTTGCTATT GGCCGGAGAG GTATTTACCG TGACCCCACT GCAGCTCTAT
    GCCGTGACGC AGTTACTCCG ACGCGCCAAG CCCACCTGTA GCACCGGCGA CGCCAGGAAC
    GACCTGGGGC ACATCATGGA TTTTGCTTGT AGACTGAAAT ACCTCCGGAT CAGTGGCCGG
    ACCGGTCCTG TTGGGACGAG TAATATCCAT GAGCAGTCTC TTCCCTGTGA CCTCTCCATC
    TTCAAATCCC TCTGTCAGAT TGAGGTCAGT CAGTGCAACG CCCGGCTCCT CAGCGGCCTC
    ACTTCCTGTA GGAGGAGTTT AGCCACCATC AGCATCCACT GCTCCGCCTC TTCCATGAAG
    GACATTCTGG TGCCGGAGGC CTATGACTTT GAGCAGTGGG AGCCGGAGGG AGTGGCCCCT
    GGGAGCCCCA TTACTGCAGT TGTGCCCAAG TGGAAAGTGC TCACAACTCT GGATCTGAGC
    CACAATCGGA TCTCCTGTAT CGATGAGTCT GTTAAACTGA TTCCTGAGAT TGAATTCTTG
    GATTTCAGTC ACAACGACAT CTCTACAATA GAAAATCTGC AGCACCTGTA CAACCTCATT
    CACTTGGACC TGTCCTACAA CAAACTGGCA GACCTGACTG GCATCTACAC TAAGGTTGGG
    AATATCAAGA CTCTCAGTCT GGCCGGGAAT GTACTGGAAA GTCTCCGCGG CCTCAATAAA
    TTGTATTCAC TTGTTAACCT GGATCTGAGC CAGAACAGAA TTGAGCAGTT GGAAGAAGTA
    AGGAACATCG GGGGGCTGCC GTGCCTGGAG GGGGTGCTCC TCGCTGGGAA CCCTCTCACT
    GTCATTCCGG ATTACAGGAC CAAAGTTCTG GCGCTGTTTG GGGACAGAGC GTCTGAGGTC
    TGTTTGGACA GTACCAGAAC CACAGAGAAA GAGCTGGACA CGGTGGAAGT GCTGAAGGCC
    ATACAGAAAT CAAGGGACGC ACGGAATAAA TTGGCTTCTT CTGAGAAAAA GATCAATGAG
    GAATTGCGAC CCTCTGCTGC TGGCTCTTCC TCCCCTGCCC GTGCTTCCTC TTCCTCTCTC
    TCTCGGCCTA TTAGCTGCAG CCAAGGTGCA GATTCTATAG ACCTTTCTTT AGCGAAAAGA
    CTGTCCCCGG GTGGAGCCCA ACAGAGCGGC TCTGGGGACC CGGCGGCCAA GCAAGAAGCG
    GAGAGAGGGA AGCCGTGTGA GGGGGATGTA CAGAGAGACG TGTCTGACCC TCCCCCGGGC
    AGTGGGACCA GTTGCCGGGA AGATGCCGTT ACGTTGGACC AATGGGGCCC CTGTCCCTGT
    ATGTCGCTGT ATCAGGGGGA CAAGGAGCTG CTCCCCTACA TTTCTGTGCT GATGGAGGGC
    GCATTGAGGG AACCCCGACA TGTCGCTGGG GAGGCGGAGA ATAAGTGGGG GCCCCACAGT
    TGCGAGTTCC ATAAGACAGA AGACGGTTAC TTTGAGATGG GGCCGGATAC CCAAACGACA
    GATGGAACCT TCCCAGCTGC CCCGGCTGTC AGTCACATAG TGTGGGGTCT CTCGTTCCAC
    AGTCGGGGGG CAGCGGAGCT CAGACCCTTC GCCTCGTGCC TCGTGCTCAC CGATACCCTC
    GTCGTCTGCT TTGAGCTGGC GCCCAACTCC GCCCCCAACT TACTGCGACT GGTTCTGTGT
    TTCGCCTATG GGGACGTCGC AGGATTTCAT TTCCCCGTCC CGGACATTTG CCTGAGAGTC
    AGTTTGGGGG ACGGGGAGGA GAGTGTCTTT GTTATGTCAG ACTTGCAGGG CTTGCAGCAA
    CTCTCTGCCT GTTTGAAAGC CCGCTGCCCC CCAAGCCACA CCCCTGAGAG GATTATTCCT
    GAACCAATGA GCTTGCAGGA TTTGCTCCTT TATATTTCTG AGGTGCAGCA AGTCGTACTG
    AGCGATAGCG ACGTAAAGGG ATGTTTCCCG GTGTATCTGC TTAACGGTGA GGCCGCCGGT
    GACCCCGCCC AACCAGAGCC TCCATTTCAT ACACAAACAT GGCCTCCAGC TGCCTCCTGT
    GCTGCACTCC TTTCCATCAT ATCTACAGGC CTTCAGCCCG GGGGGGAAAG GCTGCTCCCC
    CATTGGCTAC TCCTCACTCA GCAATGTTTC ATACTAATTA CAGTCGACAT TAGTACAATA
    ACCGGTAACC GGGCGGAATA CGCGGGAAAT GCGTTAACGA TGAGCGCCAT TCCCTTGGCT
    TCAGTTGCGT TGGAGCCGGA GAGTCCCCCT GCTGAGAATA AGCCCCCCCA GTTTGCCGGA
    CACACTGTCC CTTTAATGGT GGATTACAGA GCAGTCAGCG CATTCTTTGT TGTGCCCCAA
    AATAAATTCC ACTTCTTGTC GGTGTTTAAC CAGCTGAGAA AGTCCCTTCA GGGGATAAAG
    AACTTGGTGA TTTGCAAATC CATAAAAAAT CTGGACGATT CCCAGAGCTG GGTGGGCGAC
    GCCCACAGAC CCCCAACGCA CTCGCTAAGC CCCGTTGCGC CCCACAGGGC ACGGCCCCCC
    CAGTACCCAT CAGAGAGCCT GACACAGAGA TTATCAGAGG AAAACCACAT TCCCTTCTAC
    CTTCCTGCAT CGGCCTCGCT GCGCTTCATT GCTGAACTGA AGGGGAAGGC GCTGGTGGAG
    CTGTTCCGTA GCTGCGTTGC CGAGGCAGAG GGGGAGGAGC TGAGGCATCT CACGTGGGCA
    TCACTGCTGC TTTATGGGAC CCCCGATACA GAGTTTCCAT CCTGCATTTT ACTTTCAACA
    CAAGCTGTTT ATTTTCTCTT GGATGATACT CAGAACCCCA AAGAACAAGG TTATTGGAGG
    AAGAGACCAG CGCGCAGCCC CCTCCAGATT TCCTTCTGTT TCGCCATCAA ATTCAGCAGT
    TTACAGTCTG TCAATATCGG ACTTTTTGAT CAGTATTTCA GGATAACAGG TTCTTCCCCC
    AGTCAGGTGG TCACGTGTCT GACCAGAGAC AGTTACAGCA CCCACTCCTT CATCCAGCAG
    CTCATGTCTG CCCTGTCCCT CCTGGCACGG GCCCCGTCCC CCGAACCCCT GGAGAAGGAT
    TTCTACTCGG AATTTGGTAC AAAGAACACG GGGAGAATGG AAAACTACGA ACTGATCCAT
    TCCAGCAGAG TGAAATTCGT TTACCCAAAC GAGGAGGAGA TGGGAGACCT GACGTTCATA
    GTGTCGGAGA AGATGGACCC TTCCAGCCCC ATCCAGTCGC TAAACATTCT GCTGTACGTA
    TTGGGCTTCC GGGTCTCTTG TGTGGGCAGA ACCGACCCGG CGCCCTCCCT GCAGCCCAAC
    AGCCTCATCC TCACCAGCTC CGACCTGTTT CTGTTCAATG AGGATTATAT CAGTTACCCG
    CTGCCAGACT TTGCCAAGGA ACCCCCCAAG AAGGACAAAT ACCAGTTAGT TGATGGGAGG
    AGAATCAGGG ATTTGGACCG GGTGCTGATG GGATACCAGA CCTACCCTCA GAACCTCACC
    TTGGTGTTTG ATGATGTTCA CAAGCAGGAT TTCATGCACA GCGTCTCTCT CGATCACTTT
    GGGGAGACGC TGGGGGGCTC TCCCGGGGCC GTGGGAGCCA ATAAAGAGAT CCAGTGGAAC
    ATTTTTATCC CAAGCGCAGA CTCCCGGGAG AAGCTGATCT CTCTGCTCGC CCGCCAATGG
    GAGAGCTTGT GCGGCAGGGA GCTTCCCATC GAACTCACCG GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001123817.1
    CDS248..4132
    Translation

    Target ORF information:

    RefSeq Version NM_001130345.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis nischarin (nisch), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001130345.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGATCGGGA AGAACTCGAG AAGTTTGGTG GAAAAGCGGC AGAAAGATCT GGAATTGTAT 
    CTGCAGACGC TGCTTGGCAC GTTCCCCCTC GCTGTGCCCA AAGCCCTGGC ACGCTTCCTG
    CATTTCCACC TCTATGAAAT CCATGGAATC GCTGCAGTTC TGGCCGAGGA GCTTTTCCAT
    AAAGGGGAGC AGTTGCTATT GGCCGGAGAG GTATTTACCG TGACCCCACT GCAGCTCTAT
    GCCGTGACGC AGTTACTCCG ACGCGCCAAG CCCACCTGTA GCACCGGCGA CGCCAGGAAC
    GACCTGGGGC ACATCATGGA TTTTGCTTGT AGACTGAAAT ACCTCCGGAT CAGTGGCCGG
    ACCGGTCCTG TTGGGACGAG TAATATCCAT GAGCAGTCTC TTCCCTGTGA CCTCTCCATC
    TTCAAATCCC TCTGTCAGAT TGAGGTCAGT CAGTGCAACG CCCGGCTCCT CAGCGGCCTC
    ACTTCCTGTA GGAGGAGTTT AGCCACCATC AGCATCCACT GCTCCGCCTC TTCCATGAAG
    GACATTCTGG TGCCGGAGGC CTATGACTTT GAGCAGTGGG AGCCGGAGGG AGTGGCCCCT
    GGGAGCCCCA TTACTGCAGT TGTGCCCAAG TGGAAAGTGC TCACAACTCT GGATCTGAGC
    CACAATCGGA TCTCCTGTAT CGATGAGTCT GTTAAACTGA TTCCTGAGAT TGAATTCTTG
    GATTTCAGTC ACAACGACAT CTCTACAATA GAAAATCTGC AGCACCTGTA CAACCTCATT
    CACTTGGACC TGTCCTACAA CAAACTGGCA GACCTGACTG GCATCTACAC TAAGGTTGGG
    AATATCAAGA CTCTCAGTCT GGCCGGGAAT GTACTGGAAA GTCTCCGCGG CCTCAATAAA
    TTGTATTCAC TTGTTAACCT GGATCTGAGC CAGAACAGAA TTGAGCAGTT GGAAGAAGTA
    AGGAACATCG GGGGGCTGCC GTGCCTGGAG GGGGTGCTCC TCGCTGGGAA CCCTCTCACT
    GTCATTCCGG ATTACAGGAC CAAAGTTCTG GCGCTGTTTG GGGACAGAGC GTCTGAGGTC
    TGTTTGGACA GTACCAGAAC CACAGAGAAA GAGCTGGACA CGGTGGAAGT GCTGAAGGCC
    ATACAGAAAT CAAGGGACGC ACGGAATAAA TTGGCTTCTT CTGAGAAAAA GATCAATGAG
    GAATTGCGAC CCTCTGCTGC TGGCTCTTCC TCCCCTGCCC GTGCTTCCTC TTCCTCTCTC
    TCTCGGCCTA TTAGCTGCAG CCAAGGTGCA GATTCTATAG ACCTTTCTTT AGCGAAAAGA
    CTGTCCCCGG GTGGAGCCCA ACAGAGCGGC TCTGGGGACC CGGCGGCCAA GCAAGAAGCG
    GAGAGAGGGA AGCCGTGTGA GGGGGATGTA CAGAGAGACG TGTCTGACCC TCCCCCGGGC
    AGTGGGACCA GTTGCCGGGA AGATGCCGTT ACGTTGGACC AATGGGGCCC CTGTCCCTGT
    ATGTCGCTGT ATCAGGGGGA CAAGGAGCTG CTCCCCTACA TTTCTGTGCT GATGGAGGGC
    GCATTGAGGG AACCCCGACA TGTCGCTGGG GAGGCGGAGA ATAAGTGGGG GCCCCACAGT
    TGCGAGTTCC ATAAGACAGA AGACGGTTAC TTTGAGATGG GGCCGGATAC CCAAACGACA
    GATGGAACCT TCCCAGCTGC CCCGGCTGTC AGTCACATAG TGTGGGGTCT CTCGTTCCAC
    AGTCGGGGGG CAGCGGAGCT CAGACCCTTC GCCTCGTGCC TCGTGCTCAC CGATACCCTC
    GTCGTCTGCT TTGAGCTGGC GCCCAACTCC GCCCCCAACT TACTGCGACT GGTTCTGTGT
    TTCGCCTATG GGGACGTCGC AGGATTTCAT TTCCCCGTCC CGGACATTTG CCTGAGAGTC
    AGTTTGGGGG ACGGGGAGGA GAGTGTCTTT GTTATGTCAG ACTTGCAGGG CTTGCAGCAA
    CTCTCTGCCT GTTTGAAAGC CCGCTGCCCC CCAAGCCACA CCCCTGAGAG GATTATTCCT
    GAACCAATGA GCTTGCAGGA TTTGCTCCTT TATATTTCTG AGGTGCAGCA AGTCGTACTG
    AGCGATAGCG ACGTAAAGGG ATGTTTCCCG GTGTATCTGC TTAACGGTGA GGCCGCCGGT
    GACCCCGCCC AACCAGAGCC TCCATTTCAT ACACAAACAT GGCCTCCAGC TGCCTCCTGT
    GCTGCACTCC TTTCCATCAT ATCTACAGGC CTTCAGCCCG GGGGGGAAAG GCTGCTCCCC
    CATTGGCTAC TCCTCACTCA GCAATGTTTC ATACTAATTA CAGTCGACAT TAGTACAATA
    ACCGGTAACC GGGCGGAATA CGCGGGAAAT GCGTTAACGA TGAGCGCCAT TCCCTTGGCT
    TCAGTTGCGT TGGAGCCGGA GAGTCCCCCT GCTGAGAATA AGCCCCCCCA GTTTGCCGGA
    CACACTGTCC CTTTAATGGT GGATTACAGA GCAGTCAGCG CATTCTTTGT TGTGCCCCAA
    AATAAATTCC ACTTCTTGTC GGTGTTTAAC CAGCTGAGAA AGTCCCTTCA GGGGATAAAG
    AACTTGGTGA TTTGCAAATC CATAAAAAAT CTGGACGATT CCCAGAGCTG GGTGGGCGAC
    GCCCACAGAC CCCCAACGCA CTCGCTAAGC CCCGTTGCGC CCCACAGGGC ACGGCCCCCC
    CAGTACCCAT CAGAGAGCCT GACACAGAGA TTATCAGAGG AAAACCACAT TCCCTTCTAC
    CTTCCTGCAT CGGCCTCGCT GCGCTTCATT GCTGAACTGA AGGGGAAGGC GCTGGTGGAG
    CTGTTCCGTA GCTGCGTTGC CGAGGCAGAG GGGGAGGAGC TGAGGCATCT CACGTGGGCA
    TCACTGCTGC TTTATGGGAC CCCCGATACA GAGTTTCCAT CCTGCATTTT ACTTTCAACA
    CAAGCTGTTT ATTTTCTCTT GGATGATACT CAGAACCCCA AAGAACAAGG TTATTGGAGG
    AAGAGACCAG CGCGCAGCCC CCTCCAGATT TCCTTCTGTT TCGCCATCAA ATTCAGCAGT
    TTACAGTCTG TCAATATCGG ACTTTTTGAT CAGTATTTCA GGATAACAGG TTCTTCCCCC
    AGTCAGGTGG TCACGTGTCT GACCAGAGAC AGTTACAGCA CCCACTCCTT CATCCAGCAG
    CTCATGTCTG CCCTGTCCCT CCTGGCACGG GCCCCGTCCC CCGAACCCCT GGAGAAGGAT
    TTCTACTCGG AATTTGGTAC AAAGAACACG GGGAGAATGG AAAACTACGA ACTGATCCAT
    TCCAGCAGAG TGAAATTCGT TTACCCAAAC GAGGAGGAGA TGGGAGACCT GACGTTCATA
    GTGTCGGAGA AGATGGACCC TTCCAGCCCC ATCCAGTCGC TAAACATTCT GCTGTACGTA
    TTGGGCTTCC GGGTCTCTTG TGTGGGCAGA ACCGACCCGG CGCCCTCCCT GCAGCCCAAC
    AGCCTCATCC TCACCAGCTC CGACCTGTTT CTGTTCAATG AGGATTATAT CAGTTACCCG
    CTGCCAGACT TTGCCAAGGA ACCCCCCAAG AAGGACAAAT ACCAGTTAGT TGATGGGAGG
    AGAATCAGGG ATTTGGACCG GGTGCTGATG GGATACCAGA CCTACCCTCA GAACCTCACC
    TTGGTGTTTG ATGATGTTCA CAAGCAGGAT TTCATGCACA GCGTCTCTCT CGATCACTTT
    GGGGAGACGC TGGGGGGCTC TCCCGGGGCC GTGGGAGCCA ATAAAGAGAT CCAGTGGAAC
    ATTTTTATCC CAAGCGCAGA CTCCCGGGAG AAGCTGATCT CTCTGCTCGC CCGCCAATGG
    GAGAGCTTGT GCGGCAGGGA GCTTCCCATC GAACTCACCG GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.