col25a1.2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following col25a1.2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the col25a1.2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa02633 NM_001130275.1
Latest version!
Xenopus tropicalis collagen, type XXV, alpha 1, gene 2 (col25a1.2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OXa47626 XM_031895996.1
Latest version!
Xenopus tropicalis collagen, type XXV, alpha 1, gene 2 (col25a1.2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa47627 XM_031895998.1
Latest version!
Xenopus tropicalis collagen, type XXV, alpha 1, gene 2 (col25a1.2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa47628 XM_031896000.1
Latest version!
Xenopus tropicalis collagen, type XXV, alpha 1, gene 2 (col25a1.2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa47629 XM_031896001.1
Latest version!
Xenopus tropicalis collagen, type XXV, alpha 1, gene 2 (col25a1.2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa47630 XM_031896002.1
Latest version!
Xenopus tropicalis collagen, type XXV, alpha 1, gene 2 (col25a1.2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa47631 XM_031896004.1
Latest version!
Xenopus tropicalis collagen, type XXV, alpha 1, gene 2 (col25a1.2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa47632 XM_031896006.1
Latest version!
Xenopus tropicalis collagen, type XXV, alpha 1, gene 2 (col25a1.2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa47633 XM_031896008.1
Latest version!
Xenopus tropicalis collagen, type XXV, alpha 1, gene 2 (col25a1.2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa47634 XM_031896009.1
Latest version!
Xenopus tropicalis collagen, type XXV, alpha 1, gene 2 (col25a1.2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa47635 XM_031896010.1
Latest version!
Xenopus tropicalis collagen, type XXV, alpha 1, gene 2 (col25a1.2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa47636 XM_031896011.1
Latest version!
Xenopus tropicalis collagen, type XXV, alpha 1, gene 2 (col25a1.2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa47637 XM_031896014.1
Latest version!
Xenopus tropicalis collagen, type XXV, alpha 1, gene 2 (col25a1.2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa02633
    Clone ID Related Accession (Same CDS sequence) NM_001130275.1
    Accession Version NM_001130275.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1905bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(XXV) chain
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC167458.1. ##Evidence-Data-START## Transcript exon combination :: BC167458.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028291, SAMD00028292 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGTTGGGAA CAGGAGAGAT GAGAAAGAAT GGAGAGAAGT CCCAGGGATG TCACAGGGAC 
    AAGCTTGGAG GGGTGCTCAG TGGCTGGGTT AGGACTTTGC CCAGTGTCTT GGCATTTGTG
    TTCTCCCTGT TGTCCATGGC ATCATTTCTT TATCTGAATA TGAAGACCAA TGACCTGCAG
    GGAAGGGTTT GGGCACTGCA GCATGGCAAG TCTGGGGACA TTGCTTTCCA TATGCCAGGA
    TTATCTGTGG ATCAGCTTAA CTCCATCGTC CAGGAAAAAG TGGATCGCCT CTTGTCCCAG
    AGGTCCTATG AACACATGGC AAAGCTCCGA ATCGCAAGAG ATGCACCGGC AGACTGCAAC
    TGCCCAGCAG GCCCACCTGG ACAGCGAGGA AAAAGGGGGA GAAGAGGTGA ACCTGGACCA
    CCAGGTCCTC CTGGCCCAAA GGGAGACAAG GGTGATCAAG GTGATCAGGG ACCTCGGATG
    GTGTTTCCTA AGATCAATCA TGGCTTTCTC TCTGCTGATC AGCAGCTAAT AAAACGCCGC
    CTGATTAAGG GTGATCAAGG ACAAGCAGGC CCCCCGGGCC CCCCAGGTCC ACCGGGTCCA
    CCAGGTCCTA GGGGTCCTCC AGGAGACACT GGGAAAGATG GTCCAAGGGG ATTGTCTGGA
    GCTCCAGGGG AGACAGGGAA ACCAGGCGAA CAAGGCTTAA TGGGTCCAAT GGGGCCTCCT
    GGACAAAAGG GGTCCATTGG TGCACCTGGA ATTCCTGGAG TAGATGGTCA AAAGGGCGAG
    CAAGGAGAAG TTGGGACACC TGGCATTGAT GGAGTATCTG GAATGAAGGG TGATCCAGGT
    GAACAAGGAG ATAAGGGTGA TCCAGGGGAA GGCGGCCCTA AAGGAGATAT AGGAGCTAAG
    GGGGATCCAG GATCTTCTGC TGCGGGAATA AAGGGAGAGC CTGGAGAGCC TGGACGTTTT
    GGGCAGAAGG GTGAACCAGG GCTTCCTGGG CTTCCTGGAC TTCCTGGGAT TAAGGGTGAG
    CCAGGATTTC TAGGGCCTCA AGGAGAACCT GGATTACCAG GCTTACCAGG AACTAAGGGG
    GAACGAGGAG AAATTGGTCC ACCTGGAAGG GGAGAAAGAG GCAGTACTGG ACTAACTGGA
    CCAAAGGGGG AGCCTGGTGA ATCCGGATCT AGAGGCCCAA AGGGGTCAAA GGGAGAATCT
    GGAAGCAAAG GAGATTCTGG CCCCATTGGT CCTAGGGGGC CTCCAGGTCA AAAAGGGGAT
    CAAGGGGCTA CTGAGATCAT AGATTACAAT GGGAATATTC AGGAAGCCTT GCAGAGGATT
    ACAGCCATTA CTGTTACGGG GCCGCCTGGA CCTCCTGGAC CACCTGGTTT GCCAGGTCCA
    AAGGGTGATG GAGGTCCTGT TGGTCCTCCA GGAGTTGATG GGGAGCAGGG TCCAAAAGGA
    TCAAAGGGTG ACCATGGTGA TTCCGGGACA TCTGGAGAGA AGGGAGGAAT TGGTCTCCCT
    GGTTTGCCGG GTGCAAATGG AATGAAAGGA GAGAAAGGAG ATCATGGTTC CCCTGGGCCC
    CATGGCGCTT CTATCATTGG CCCCCCTGGA CCTCCAGGGC CACATGGCCC CCCTGGACCC
    ATGGGACCAC ATGGACTTAC AGGGCCAAAG GGGAATGATG GTCCCATTGG TGCTTCTGGT
    CCAGCAGGTC CAAAAGGAGA CAGAGGAGAA AAAGGAGTTG TTGGTGATCC CGGACCCAGA
    GGGCCTTATG GTTTACCTGG CAAAGATGGA GAGCCTGGTC TAGACGGTTT CCGTGGATTA
    AAGGGGGAAA AAGGGGAACC AGGTCAGCCT GGCCTGGATG GGCTGGATGC CCCTTGCCAA
    TTGGGACCAG ATGGGCTTCC AATGCCTGGT TGTTGGCAAA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001123747.1
    CDS443..2347
    Translation

    Target ORF information:

    RefSeq Version NM_001130275.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type XXV, alpha 1, gene 2 (col25a1.2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001130275.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGTTGGGAA CAGGAGAGAT GAGAAAGAAT GGAGAGAAGT CCCAGGGATG TCACAGGGAC 
    AAGCTTGGAG GGGTGCTCAG TGGCTGGGTT AGGACTTTGC CCAGTGTCTT GGCATTTGTG
    TTCTCCCTGT TGTCCATGGC ATCATTTCTT TATCTGAATA TGAAGACCAA TGACCTGCAG
    GGAAGGGTTT GGGCACTGCA GCATGGCAAG TCTGGGGACA TTGCTTTCCA TATGCCAGGA
    TTATCTGTGG ATCAGCTTAA CTCCATCGTC CAGGAAAAAG TGGATCGCCT CTTGTCCCAG
    AGGTCCTATG AACACATGGC AAAGCTCCGA ATCGCAAGAG ATGCACCGGC AGACTGCAAC
    TGCCCAGCAG GCCCACCTGG ACAGCGAGGA AAAAGGGGGA GAAGAGGTGA ACCTGGACCA
    CCAGGTCCTC CTGGCCCAAA GGGAGACAAG GGTGATCAAG GTGATCAGGG ACCTCGGATG
    GTGTTTCCTA AGATCAATCA TGGCTTTCTC TCTGCTGATC AGCAGCTAAT AAAACGCCGC
    CTGATTAAGG GTGATCAAGG ACAAGCAGGC CCCCCGGGCC CCCCAGGTCC ACCGGGTCCA
    CCAGGTCCTA GGGGTCCTCC AGGAGACACT GGGAAAGATG GTCCAAGGGG ATTGTCTGGA
    GCTCCAGGGG AGACAGGGAA ACCAGGCGAA CAAGGCTTAA TGGGTCCAAT GGGGCCTCCT
    GGACAAAAGG GGTCCATTGG TGCACCTGGA ATTCCTGGAG TAGATGGTCA AAAGGGCGAG
    CAAGGAGAAG TTGGGACACC TGGCATTGAT GGAGTATCTG GAATGAAGGG TGATCCAGGT
    GAACAAGGAG ATAAGGGTGA TCCAGGGGAA GGCGGCCCTA AAGGAGATAT AGGAGCTAAG
    GGGGATCCAG GATCTTCTGC TGCGGGAATA AAGGGAGAGC CTGGAGAGCC TGGACGTTTT
    GGGCAGAAGG GTGAACCAGG GCTTCCTGGG CTTCCTGGAC TTCCTGGGAT TAAGGGTGAG
    CCAGGATTTC TAGGGCCTCA AGGAGAACCT GGATTACCAG GCTTACCAGG AACTAAGGGG
    GAACGAGGAG AAATTGGTCC ACCTGGAAGG GGAGAAAGAG GCAGTACTGG ACTAACTGGA
    CCAAAGGGGG AGCCTGGTGA ATCCGGATCT AGAGGCCCAA AGGGGTCAAA GGGAGAATCT
    GGAAGCAAAG GAGATTCTGG CCCCATTGGT CCTAGGGGGC CTCCAGGTCA AAAAGGGGAT
    CAAGGGGCTA CTGAGATCAT AGATTACAAT GGGAATATTC AGGAAGCCTT GCAGAGGATT
    ACAGCCATTA CTGTTACGGG GCCGCCTGGA CCTCCTGGAC CACCTGGTTT GCCAGGTCCA
    AAGGGTGATG GAGGTCCTGT TGGTCCTCCA GGAGTTGATG GGGAGCAGGG TCCAAAAGGA
    TCAAAGGGTG ACCATGGTGA TTCCGGGACA TCTGGAGAGA AGGGAGGAAT TGGTCTCCCT
    GGTTTGCCGG GTGCAAATGG AATGAAAGGA GAGAAAGGAG ATCATGGTTC CCCTGGGCCC
    CATGGCGCTT CTATCATTGG CCCCCCTGGA CCTCCAGGGC CACATGGCCC CCCTGGACCC
    ATGGGACCAC ATGGACTTAC AGGGCCAAAG GGGAATGATG GTCCCATTGG TGCTTCTGGT
    CCAGCAGGTC CAAAAGGAGA CAGAGGAGAA AAAGGAGTTG TTGGTGATCC CGGACCCAGA
    GGGCCTTATG GTTTACCTGG CAAAGATGGA GAGCCTGGTC TAGACGGTTT CCGTGGATTA
    AAGGGGGAAA AAGGGGAACC AGGTCAGCCT GGCCTGGATG GGCTGGATGC CCCTTGCCAA
    TTGGGACCAG ATGGGCTTCC AATGCCTGGT TGTTGGCAAA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47626
    Clone ID Related Accession (Same CDS sequence) XM_031895996.1
    Accession Version XM_031895996.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2118bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(XXV) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTTGGGAA CAGGAGAGAT GAGAAAGAAT GGAGAGAAGT CCCAGGGATG TCACAGGGAC 
    AAGCTTGGAG GGGTGCTCAG TGGCTGGGTT AGGACTTTGC CCAGTGTCTT GGCATTTGTG
    TTCTCCCTGT TGTCCATGGC ATCATTTCTT TATCTGAATA TGAAGACCAA TGACCTGCAG
    GGAAGGGTTT GGGCACTGCA GCATGGCAAG TCTGGGGACA TTGCTTTCCA TATGCCAGGA
    TTATCTGTGG ATCAGCTTAA CTCCATCGTC CAGGAAAAAG TGGATCGCCT CTTGTCCCAG
    AGGTCCTATG AACACATGGC AAAGCTCCGA ATCGCAAGAG ATGCACCGGC AGACTGCAAC
    TGCCCAGCAG GCCCACCTGG ACAGCGAGGA AAAAGGGGGA GAAGAGGTGA ACCTGGACCA
    CCAGGTCCTC CTGGCCCAAA GGGAGACAAG GGTGATCAAG GTGATCAGGG ACCTCGGATG
    GTGTTTCCTA AGATCAATCA TGGCTTTCTC TCTGCTGATC AGCAGCTAAT AAAACGCCGC
    CTGATTAAGG GTGATCAAGG ACAAGCAGGC CCCCCGGGCC CCCCAGGTCC ACCGGGTCCA
    CCAGGTCCTA GGGGTCCTCC AGGAGACACT GGGAAAGATG GTCCAAGGGG ATTGTCTGGA
    GCTCCAGGGG AGACAGGGAA ACCAGGCGAA CAAGGCTTAA TGGGTCCAAT GGGGCCTCCT
    GGACAAAAGG GGTCCATTGG TGCACCTGGA ATTCCTGGAG TAGATGGTCA AAAGGGCGAG
    CAAGGAGAAG TTGGGACACC TGGCATTGAT GGAGTATCTG GAATGAAGGG TGATCCAGGT
    GAACAAGGAG ATAAGGGTGA TCCAGGGGAA GGCGGCCCTA AAGGAGATAT AGGAGCTAAG
    GGGGATCCAG GATCTTCTGC TGCGGGAATA AAGGGAGAGC CTGGAGAGCC TGGACGTTTT
    GGGCAGAAGG GTGAACCAGG GCTTCCTGGG CTTCCTGGAC TTCCTGGGAT TAAGGGTGAG
    CCAGGATTTC TAGGGCCTCA AGGAGAACCT GGATTACCAG GCTTACCAGG AACTAAGGGG
    GAACGAGGAG AAATTGGTCC ACCTGGAAGG GGAGAAAGAG GCAGTACTGG ACTAACTGGA
    CCAAAGGGGG AGCCTGGTGA ATCCGGATCT AGAGGCCCAA AGGGGTCAAA GGGAGAATCT
    GGAAGCAAAG GAGATTCTGG CCCCATTGGT CCTAGGGGGC CTCCAGGTCA AAAAGGGGAT
    CAAGGGGCTA CTGAGATCAT AGATTACAAT GGGAATATTC AGGAAGCCTT GCAGAGGATT
    ACAGCCATTA CTGTTACGGG GCCGCCTGGA CCTCCTGGAC CACCTGGTTT GCCAGGTCCA
    AAGGGTGATG GAGGTCCTGT TGGTCCTCCA GGAGTTGATG GGGAGCAGGG TCCAAAAGGA
    TCAAAGGGTG ACCATGGTGA TTCCGGGACA TCTGGAGAGA AGGGAGGAAT TGGTCTCCCT
    GGTTTGCCGG GTGCAAATGG AATGAAAGGA GAGAAAGGAG ATCATGGTTC CCCTGGGCCC
    CATGGCGCTT CTATCATTGG CCCCCCTGGA CCTCCAGGGC CACATGGCCC CCCTGGACCC
    ATGGGACCAC ATGGACTTAC AGGGCCAAAG GGTGAACCAG GGGTAAATGG TCTTAAAGGA
    TTGAGGGGTG AGCCAGGTCA AAAGGGTGAA AGAGGACCAC TGGGTCTTCC AGGAGCATCT
    GGCTTAGACG GAAAGCCAGG ACCCAGGGGG AATGATGGTC CCATTGGTGC TTCTGGTCCA
    GCAGGTCCAA AAGGAGACAG AGGAGAAAAA GGAGTTGTTG GTGATCCCGG ACCCAGAGGG
    CCTTATGGTT TACCTGGCAA AGATGGAGAG CCTGGTCTAG ACGGTTTTCC GGGTCCTCGT
    GGAGAAAAAG GTGACCCTGG AGAAAGGGGA GAAAAGGGGG AAAAGGGAAA GAAAGGCAAA
    AAAGGGCCTA AAGGGGAGAA AGGAGAACAG GGTGCCCCTG GATTAGATGC ACCTTGCCCA
    TTGGGACCAG ATGGGCTTCC AATGCCTGGT TGTTGGCAAA AGGGAGTGAC CCCATGGCTT
    GTTGCCAAAA AGAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751856.1
    CDS480..2597
    Translation

    Target ORF information:

    RefSeq Version XM_031895996.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type XXV, alpha 1, gene 2 (col25a1.2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031895996.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTTGGGAA CAGGAGAGAT GAGAAAGAAT GGAGAGAAGT CCCAGGGATG TCACAGGGAC 
    AAGCTTGGAG GGGTGCTCAG TGGCTGGGTT AGGACTTTGC CCAGTGTCTT GGCATTTGTG
    TTCTCCCTGT TGTCCATGGC ATCATTTCTT TATCTGAATA TGAAGACCAA TGACCTGCAG
    GGAAGGGTTT GGGCACTGCA GCATGGCAAG TCTGGGGACA TTGCTTTCCA TATGCCAGGA
    TTATCTGTGG ATCAGCTTAA CTCCATCGTC CAGGAAAAAG TGGATCGCCT CTTGTCCCAG
    AGGTCCTATG AACACATGGC AAAGCTCCGA ATCGCAAGAG ATGCACCGGC AGACTGCAAC
    TGCCCAGCAG GCCCACCTGG ACAGCGAGGA AAAAGGGGGA GAAGAGGTGA ACCTGGACCA
    CCAGGTCCTC CTGGCCCAAA GGGAGACAAG GGTGATCAAG GTGATCAGGG ACCTCGGATG
    GTGTTTCCTA AGATCAATCA TGGCTTTCTC TCTGCTGATC AGCAGCTAAT AAAACGCCGC
    CTGATTAAGG GTGATCAAGG ACAAGCAGGC CCCCCGGGCC CCCCAGGTCC ACCGGGTCCA
    CCAGGTCCTA GGGGTCCTCC AGGAGACACT GGGAAAGATG GTCCAAGGGG ATTGTCTGGA
    GCTCCAGGGG AGACAGGGAA ACCAGGCGAA CAAGGCTTAA TGGGTCCAAT GGGGCCTCCT
    GGACAAAAGG GGTCCATTGG TGCACCTGGA ATTCCTGGAG TAGATGGTCA AAAGGGCGAG
    CAAGGAGAAG TTGGGACACC TGGCATTGAT GGAGTATCTG GAATGAAGGG TGATCCAGGT
    GAACAAGGAG ATAAGGGTGA TCCAGGGGAA GGCGGCCCTA AAGGAGATAT AGGAGCTAAG
    GGGGATCCAG GATCTTCTGC TGCGGGAATA AAGGGAGAGC CTGGAGAGCC TGGACGTTTT
    GGGCAGAAGG GTGAACCAGG GCTTCCTGGG CTTCCTGGAC TTCCTGGGAT TAAGGGTGAG
    CCAGGATTTC TAGGGCCTCA AGGAGAACCT GGATTACCAG GCTTACCAGG AACTAAGGGG
    GAACGAGGAG AAATTGGTCC ACCTGGAAGG GGAGAAAGAG GCAGTACTGG ACTAACTGGA
    CCAAAGGGGG AGCCTGGTGA ATCCGGATCT AGAGGCCCAA AGGGGTCAAA GGGAGAATCT
    GGAAGCAAAG GAGATTCTGG CCCCATTGGT CCTAGGGGGC CTCCAGGTCA AAAAGGGGAT
    CAAGGGGCTA CTGAGATCAT AGATTACAAT GGGAATATTC AGGAAGCCTT GCAGAGGATT
    ACAGCCATTA CTGTTACGGG GCCGCCTGGA CCTCCTGGAC CACCTGGTTT GCCAGGTCCA
    AAGGGTGATG GAGGTCCTGT TGGTCCTCCA GGAGTTGATG GGGAGCAGGG TCCAAAAGGA
    TCAAAGGGTG ACCATGGTGA TTCCGGGACA TCTGGAGAGA AGGGAGGAAT TGGTCTCCCT
    GGTTTGCCGG GTGCAAATGG AATGAAAGGA GAGAAAGGAG ATCATGGTTC CCCTGGGCCC
    CATGGCGCTT CTATCATTGG CCCCCCTGGA CCTCCAGGGC CACATGGCCC CCCTGGACCC
    ATGGGACCAC ATGGACTTAC AGGGCCAAAG GGTGAACCAG GGGTAAATGG TCTTAAAGGA
    TTGAGGGGTG AGCCAGGTCA AAAGGGTGAA AGAGGACCAC TGGGTCTTCC AGGAGCATCT
    GGCTTAGACG GAAAGCCAGG ACCCAGGGGG AATGATGGTC CCATTGGTGC TTCTGGTCCA
    GCAGGTCCAA AAGGAGACAG AGGAGAAAAA GGAGTTGTTG GTGATCCCGG ACCCAGAGGG
    CCTTATGGTT TACCTGGCAA AGATGGAGAG CCTGGTCTAG ACGGTTTTCC GGGTCCTCGT
    GGAGAAAAAG GTGACCCTGG AGAAAGGGGA GAAAAGGGGG AAAAGGGAAA GAAAGGCAAA
    AAAGGGCCTA AAGGGGAGAA AGGAGAACAG GGTGCCCCTG GATTAGATGC ACCTTGCCCA
    TTGGGACCAG ATGGGCTTCC AATGCCTGGT TGTTGGCAAA AGGGAGTGAC CCCATGGCTT
    GTTGCCAAAA AGAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47627
    Clone ID Related Accession (Same CDS sequence) XM_031895998.1
    Accession Version XM_031895998.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2109bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(XXV) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTTGGGAA CAGGAGAGAT GAGAAAGAAT GGAGAGAAGT CCCAGGGATG TCACAGGGAC 
    AAGCTTGGAG GGGTGCTCAG TGGCTGGGTT AGGACTTTGC CCAGTGTCTT GGCATTTGTG
    TTCTCCCTGT TGTCCATGGC ATCATTTCTT TATCTGAATA TGAAGACCAA TGACCTGCAG
    GGAAGGGTTT GGGCACTGCA GCATGGCAAG TCTGGGGACA TTGCTTTCCA TATGCCAGGA
    TTATCTGTGG ATCAGCTTAA CTCCATCGTC CAGGAAAAAG TGGATCGCCT CTTGTCCCAG
    AGGTCCTATG AACACATGGC AAAGCTCCGA ATCGCAAGAG ATGCACCGGC AGACTGCAAC
    TGCCCAGCAG GCCCACCTGG ACAGCGAGGA AAAAGGGGGA GAAGAGGTGA ACCTGGACCA
    CCAGGTCCTC CTGGCCCAAA GGGAGACAAG GGTGATCAAG GTGATCAGGG ACCTCGGATG
    GTGTTTCCTA AGATCAATCA TGGCTTTCTC TCTGCTGATC AGCAGCTAAT AAAACGCCGC
    CTGATTAAGG GTGATCAAGG ACAAGCAGGC CCCCCGGGCC CCCCAGGTCC ACCGGGTCCA
    CCAGGTCCTA GGGGTCCTCC AGGAGACACT GGGAAAGATG GTCCAAGGGG ATTGTCTGGA
    GCTCCAGGGG AGACAGGGAA ACCAGGCGAA CAAGGCTTAA TGGGTCCAAT GGGGCCTCCT
    GGACAAAAGG GGTCCATTGG TGCACCTGGA ATTCCTGGAG TAGATGGTCA AAAGGGCGAG
    CAAGGAGAAG TTGGGACACC TGGCATTGAT GGAGTATCTG GAATGAAGGG TGATCCAGGT
    GAACAAGGAG ATAAGGGTGA TCCAGGGGAA GGCGGCCCTA AAGGAGATAT AGGAGCTAAG
    GGGGATCCAG GATCTTCTGC TGCGGGAATA AAGGGAGAGC CTGGAGAGCC TGGACGTTTT
    GGGCAGAAGG GTGAACCAGG GCTTCCTGGG CTTCCTGGAC TTCCTGGGAT TAAGGGTGAG
    CCAGGATTTC TAGGGCCTCA AGGAGAACCT GGATTACCAG GCTTACCAGG AACTAAGGGG
    GAACGAGGAG AAATTGGTCC ACCTGGAAGG GGAGAAAGAG GCAGTACTGG ACTAACTGGA
    CCAAAGGGGG AGCCTGGTGA ATCCGGATCT AGAGGCCCAA AGGGGTCAAA GGGAGAATCT
    GGAAGCAAAG GAGATTCTGG CCCCATTGGT CCTAGGGGGC CTCCAGGTCA AAAAGGGGAT
    CAAGGGGCTA CTGAGATCAT AGATTACAAT GGGAATATTC AGGAAGCCTT GCAGAGGATT
    ACAGCCATTA CTGTTACGGG GCCGCCTGGA CCTCCTGGAC CACCTGGTTT GCCAGGTCCA
    AAGGGTGATG GAGGTCCTGT TGGTCCTCCA GGAGTTGATG GGGAGCAGGG TCCAAAAGGA
    TCAAAGGGTG ACCATGGTGA TTCCGGGACA TCTGGAGAGA AGGGAGGAAT TGGTCTCCCT
    GGTTTGCCGG GTGCAAATGG AATGAAAGGA GAGAAAGGAG ATCATGGTTC CCCTGGGCCC
    CATGGCGCTT CTATCATTGG CCCCCCTGGA CCTCCAGGGC CACATGGCCC CCCTGGACCC
    ATGGGACCAC ATGGACTTAC AGGGCCAAAG GGTGAACCAG GGGTAAATGG TCTTAAAGGA
    TTGAGGGGTG AGCCAGGTCA AAAGGGTGAA AGAGGACCAC TGGGTCTTCC AGGAGCATCT
    GGCTTAGACG GAAAGCCAGG ACCCAGGGGG AATGATGGTC CCATTGGTGC TTCTGGTCCA
    GCAGGTCCAA AAGGAGACAG AGGAGAAAAA GGAGTTGTTG GTGATCCCGG ACCCAGAGGG
    CCTTATGGTT TACCTGGCAA AGATGGAGAG CCTGGTCTAG ACGGTTTTCC GGGTCCTCGT
    GGAGAAAAAG GTGACCCTGG AGAAAGGGGA GAAAAGGGTT TCCGTGGATT AAAGGGGGAA
    AAAGGGGAAC CAGGTCAGCC TGGCCTGGAT GGGCTGGATG CCCCTTGCCA ATTGGGACCA
    GATGGGCTTC CAATGCCTGG TTGTTGGCAA AAGGGAGTGA CCCCATGGCT TGTTGCCAAA
    AAGAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751858.1
    CDS480..2588
    Translation

    Target ORF information:

    RefSeq Version XM_031895998.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type XXV, alpha 1, gene 2 (col25a1.2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031895998.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTTGGGAA CAGGAGAGAT GAGAAAGAAT GGAGAGAAGT CCCAGGGATG TCACAGGGAC 
    AAGCTTGGAG GGGTGCTCAG TGGCTGGGTT AGGACTTTGC CCAGTGTCTT GGCATTTGTG
    TTCTCCCTGT TGTCCATGGC ATCATTTCTT TATCTGAATA TGAAGACCAA TGACCTGCAG
    GGAAGGGTTT GGGCACTGCA GCATGGCAAG TCTGGGGACA TTGCTTTCCA TATGCCAGGA
    TTATCTGTGG ATCAGCTTAA CTCCATCGTC CAGGAAAAAG TGGATCGCCT CTTGTCCCAG
    AGGTCCTATG AACACATGGC AAAGCTCCGA ATCGCAAGAG ATGCACCGGC AGACTGCAAC
    TGCCCAGCAG GCCCACCTGG ACAGCGAGGA AAAAGGGGGA GAAGAGGTGA ACCTGGACCA
    CCAGGTCCTC CTGGCCCAAA GGGAGACAAG GGTGATCAAG GTGATCAGGG ACCTCGGATG
    GTGTTTCCTA AGATCAATCA TGGCTTTCTC TCTGCTGATC AGCAGCTAAT AAAACGCCGC
    CTGATTAAGG GTGATCAAGG ACAAGCAGGC CCCCCGGGCC CCCCAGGTCC ACCGGGTCCA
    CCAGGTCCTA GGGGTCCTCC AGGAGACACT GGGAAAGATG GTCCAAGGGG ATTGTCTGGA
    GCTCCAGGGG AGACAGGGAA ACCAGGCGAA CAAGGCTTAA TGGGTCCAAT GGGGCCTCCT
    GGACAAAAGG GGTCCATTGG TGCACCTGGA ATTCCTGGAG TAGATGGTCA AAAGGGCGAG
    CAAGGAGAAG TTGGGACACC TGGCATTGAT GGAGTATCTG GAATGAAGGG TGATCCAGGT
    GAACAAGGAG ATAAGGGTGA TCCAGGGGAA GGCGGCCCTA AAGGAGATAT AGGAGCTAAG
    GGGGATCCAG GATCTTCTGC TGCGGGAATA AAGGGAGAGC CTGGAGAGCC TGGACGTTTT
    GGGCAGAAGG GTGAACCAGG GCTTCCTGGG CTTCCTGGAC TTCCTGGGAT TAAGGGTGAG
    CCAGGATTTC TAGGGCCTCA AGGAGAACCT GGATTACCAG GCTTACCAGG AACTAAGGGG
    GAACGAGGAG AAATTGGTCC ACCTGGAAGG GGAGAAAGAG GCAGTACTGG ACTAACTGGA
    CCAAAGGGGG AGCCTGGTGA ATCCGGATCT AGAGGCCCAA AGGGGTCAAA GGGAGAATCT
    GGAAGCAAAG GAGATTCTGG CCCCATTGGT CCTAGGGGGC CTCCAGGTCA AAAAGGGGAT
    CAAGGGGCTA CTGAGATCAT AGATTACAAT GGGAATATTC AGGAAGCCTT GCAGAGGATT
    ACAGCCATTA CTGTTACGGG GCCGCCTGGA CCTCCTGGAC CACCTGGTTT GCCAGGTCCA
    AAGGGTGATG GAGGTCCTGT TGGTCCTCCA GGAGTTGATG GGGAGCAGGG TCCAAAAGGA
    TCAAAGGGTG ACCATGGTGA TTCCGGGACA TCTGGAGAGA AGGGAGGAAT TGGTCTCCCT
    GGTTTGCCGG GTGCAAATGG AATGAAAGGA GAGAAAGGAG ATCATGGTTC CCCTGGGCCC
    CATGGCGCTT CTATCATTGG CCCCCCTGGA CCTCCAGGGC CACATGGCCC CCCTGGACCC
    ATGGGACCAC ATGGACTTAC AGGGCCAAAG GGTGAACCAG GGGTAAATGG TCTTAAAGGA
    TTGAGGGGTG AGCCAGGTCA AAAGGGTGAA AGAGGACCAC TGGGTCTTCC AGGAGCATCT
    GGCTTAGACG GAAAGCCAGG ACCCAGGGGG AATGATGGTC CCATTGGTGC TTCTGGTCCA
    GCAGGTCCAA AAGGAGACAG AGGAGAAAAA GGAGTTGTTG GTGATCCCGG ACCCAGAGGG
    CCTTATGGTT TACCTGGCAA AGATGGAGAG CCTGGTCTAG ACGGTTTTCC GGGTCCTCGT
    GGAGAAAAAG GTGACCCTGG AGAAAGGGGA GAAAAGGGTT TCCGTGGATT AAAGGGGGAA
    AAAGGGGAAC CAGGTCAGCC TGGCCTGGAT GGGCTGGATG CCCCTTGCCA ATTGGGACCA
    GATGGGCTTC CAATGCCTGG TTGTTGGCAA AAGGGAGTGA CCCCATGGCT TGTTGCCAAA
    AAGAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47628
    Clone ID Related Accession (Same CDS sequence) XM_031896000.1
    Accession Version XM_031896000.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2106bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(XXV) chain isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTTGGGAA CAGGAGAGAT GAGAAAGAAT GGAGAGAAGT CCCAGGGATG TCACAGGGAC 
    AAGCTTGGAG GGGTGCTCAG TGGCTGGGTT AGGACTTTGC CCAGTGTCTT GGCATTTGTG
    TTCTCCCTGT TGTCCATGGC ATCATTTCTT TATCTGAATA TGAAGACCAA TGACCTGCAG
    GGAAGGGTTT GGGCACTGCA GCATGGCAAG TCTGGGGACA TTGCTTTCCA TATGCCAGGA
    TTATCTGTGG ATCAGCTTAA CTCCATCGTC CAGGAAAAAG TGGATCGCCT CTTGTCCCAG
    AGGTCCTATG AACACATGGC AAAGCTCCGA ATCGCAAGAG ATGCACCGGC AGACTGCAAC
    TGCCCAGCAG GCCCACCTGG ACAGCGAGGA AAAAGGGGGA GAAGAGGTGA ACCTGGACCA
    CCAGGTCCTC CTGGCCCAAA GGGAGACAAG GGTGATCAAG GTGATCAGGG ACCTCGGGGT
    TTGCCAGGCT GTCCTACAAT GGCTGCTCTC CATAGTAATC AGATCCTCAC AGTCAAGGGT
    GATCAAGGAC AAGCAGGCCC CCCGGGCCCC CCAGGTCCAC CGGGTCCACC AGGTCCTAGG
    GGTCCTCCAG GAGACACTGG GAAAGATGGT CCAAGGGGAT TGTCTGGAGC TCCAGGGGAG
    ACAGGGAAAC CAGGCGAACA AGGCTTAATG GGTCCAATGG GGCCTCCTGG ACAAAAGGGG
    TCCATTGGTG CACCTGGAAT TCCTGGAGTA GATGGTCAAA AGGGCGAGCA AGGAGAAGTT
    GGGACACCTG GCATTGATGG AGTATCTGGA ATGAAGGGTG ATCCAGGTGA ACAAGGAGAT
    AAGGGTGATC CAGGGGAAGG CGGCCCTAAA GGAGATATAG GAGCTAAGGG GGATCCAGGA
    TCTTCTGCTG CGGGAATAAA GGGAGAGCCT GGAGAGCCTG GACGTTTTGG GCAGAAGGGT
    GAACCAGGGC TTCCTGGGCT TCCTGGACTT CCTGGGATTA AGGGTGAGCC AGGATTTCTA
    GGGCCTCAAG GAGAACCTGG ATTACCAGGC TTACCAGGAA CTAAGGGGGA ACGAGGAGAA
    ATTGGTCCAC CTGGAAGGGG AGAAAGAGGC AGTACTGGAC TAACTGGACC AAAGGGGGAG
    CCTGGTGAAT CCGGATCTAG AGGCCCAAAG GGGTCAAAGG GAGAATCTGG AAGCAAAGGA
    GATTCTGGCC CCATTGGTCC TAGGGGGCCT CCAGGTCAAA AAGGGGATCA AGGGGCTACT
    GAGATCATAG ATTACAATGG GAATATTCAG GAAGCCTTGC AGAGGATTAC AGCCATTACT
    GTTACGGGGC CGCCTGGACC TCCTGGACCA CCTGGTTTGC CAGGTCCAAA GGGTGATGGA
    GGTCCTGTTG GTCCTCCAGG AGTTGATGGG GAGCAGGGTC CAAAAGGATC AAAGGGTGAC
    CATGGTGATT CCGGGACATC TGGAGAGAAG GGAGGAATTG GTCTCCCTGG TTTGCCGGGT
    GCAAATGGAA TGAAAGGAGA GAAAGGAGAT CATGGTTCCC CTGGGCCCCA TGGCGCTTCT
    ATCATTGGCC CCCCTGGACC TCCAGGGCCA CATGGCCCCC CTGGACCCAT GGGACCACAT
    GGACTTACAG GGCCAAAGGG TGAACCAGGG GTAAATGGTC TTAAAGGATT GAGGGGTGAG
    CCAGGTCAAA AGGGTGAAAG AGGACCACTG GGTCTTCCAG GAGCATCTGG CTTAGACGGA
    AAGCCAGGAC CCAGGGGGAA TGATGGTCCC ATTGGTGCTT CTGGTCCAGC AGGTCCAAAA
    GGAGACAGAG GAGAAAAAGG AGTTGTTGGT GATCCCGGAC CCAGAGGGCC TTATGGTTTA
    CCTGGCAAAG ATGGAGAGCC TGGTCTAGAC GGTTTTCCGG GTCCTCGTGG AGAAAAAGGT
    GACCCTGGAG AAAGGGGAGA AAAGGGGGAA AAGGGAAAGA AAGGCAAAAA AGGGCCTAAA
    GGGGAGAAAG GAGAACAGGG TGCCCCTGGA TTAGATGCAC CTTGCCCATT GGGACCAGAT
    GGGCTTCCAA TGCCTGGTTG TTGGCAAAAG GGAGTGACCC CATGGCTTGT TGCCAAAAAG
    AGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751860.1
    CDS480..2585
    Translation

    Target ORF information:

    RefSeq Version XM_031896000.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type XXV, alpha 1, gene 2 (col25a1.2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896000.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTTGGGAA CAGGAGAGAT GAGAAAGAAT GGAGAGAAGT CCCAGGGATG TCACAGGGAC 
    AAGCTTGGAG GGGTGCTCAG TGGCTGGGTT AGGACTTTGC CCAGTGTCTT GGCATTTGTG
    TTCTCCCTGT TGTCCATGGC ATCATTTCTT TATCTGAATA TGAAGACCAA TGACCTGCAG
    GGAAGGGTTT GGGCACTGCA GCATGGCAAG TCTGGGGACA TTGCTTTCCA TATGCCAGGA
    TTATCTGTGG ATCAGCTTAA CTCCATCGTC CAGGAAAAAG TGGATCGCCT CTTGTCCCAG
    AGGTCCTATG AACACATGGC AAAGCTCCGA ATCGCAAGAG ATGCACCGGC AGACTGCAAC
    TGCCCAGCAG GCCCACCTGG ACAGCGAGGA AAAAGGGGGA GAAGAGGTGA ACCTGGACCA
    CCAGGTCCTC CTGGCCCAAA GGGAGACAAG GGTGATCAAG GTGATCAGGG ACCTCGGGGT
    TTGCCAGGCT GTCCTACAAT GGCTGCTCTC CATAGTAATC AGATCCTCAC AGTCAAGGGT
    GATCAAGGAC AAGCAGGCCC CCCGGGCCCC CCAGGTCCAC CGGGTCCACC AGGTCCTAGG
    GGTCCTCCAG GAGACACTGG GAAAGATGGT CCAAGGGGAT TGTCTGGAGC TCCAGGGGAG
    ACAGGGAAAC CAGGCGAACA AGGCTTAATG GGTCCAATGG GGCCTCCTGG ACAAAAGGGG
    TCCATTGGTG CACCTGGAAT TCCTGGAGTA GATGGTCAAA AGGGCGAGCA AGGAGAAGTT
    GGGACACCTG GCATTGATGG AGTATCTGGA ATGAAGGGTG ATCCAGGTGA ACAAGGAGAT
    AAGGGTGATC CAGGGGAAGG CGGCCCTAAA GGAGATATAG GAGCTAAGGG GGATCCAGGA
    TCTTCTGCTG CGGGAATAAA GGGAGAGCCT GGAGAGCCTG GACGTTTTGG GCAGAAGGGT
    GAACCAGGGC TTCCTGGGCT TCCTGGACTT CCTGGGATTA AGGGTGAGCC AGGATTTCTA
    GGGCCTCAAG GAGAACCTGG ATTACCAGGC TTACCAGGAA CTAAGGGGGA ACGAGGAGAA
    ATTGGTCCAC CTGGAAGGGG AGAAAGAGGC AGTACTGGAC TAACTGGACC AAAGGGGGAG
    CCTGGTGAAT CCGGATCTAG AGGCCCAAAG GGGTCAAAGG GAGAATCTGG AAGCAAAGGA
    GATTCTGGCC CCATTGGTCC TAGGGGGCCT CCAGGTCAAA AAGGGGATCA AGGGGCTACT
    GAGATCATAG ATTACAATGG GAATATTCAG GAAGCCTTGC AGAGGATTAC AGCCATTACT
    GTTACGGGGC CGCCTGGACC TCCTGGACCA CCTGGTTTGC CAGGTCCAAA GGGTGATGGA
    GGTCCTGTTG GTCCTCCAGG AGTTGATGGG GAGCAGGGTC CAAAAGGATC AAAGGGTGAC
    CATGGTGATT CCGGGACATC TGGAGAGAAG GGAGGAATTG GTCTCCCTGG TTTGCCGGGT
    GCAAATGGAA TGAAAGGAGA GAAAGGAGAT CATGGTTCCC CTGGGCCCCA TGGCGCTTCT
    ATCATTGGCC CCCCTGGACC TCCAGGGCCA CATGGCCCCC CTGGACCCAT GGGACCACAT
    GGACTTACAG GGCCAAAGGG TGAACCAGGG GTAAATGGTC TTAAAGGATT GAGGGGTGAG
    CCAGGTCAAA AGGGTGAAAG AGGACCACTG GGTCTTCCAG GAGCATCTGG CTTAGACGGA
    AAGCCAGGAC CCAGGGGGAA TGATGGTCCC ATTGGTGCTT CTGGTCCAGC AGGTCCAAAA
    GGAGACAGAG GAGAAAAAGG AGTTGTTGGT GATCCCGGAC CCAGAGGGCC TTATGGTTTA
    CCTGGCAAAG ATGGAGAGCC TGGTCTAGAC GGTTTTCCGG GTCCTCGTGG AGAAAAAGGT
    GACCCTGGAG AAAGGGGAGA AAAGGGGGAA AAGGGAAAGA AAGGCAAAAA AGGGCCTAAA
    GGGGAGAAAG GAGAACAGGG TGCCCCTGGA TTAGATGCAC CTTGCCCATT GGGACCAGAT
    GGGCTTCCAA TGCCTGGTTG TTGGCAAAAG GGAGTGACCC CATGGCTTGT TGCCAAAAAG
    AGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47629
    Clone ID Related Accession (Same CDS sequence) XM_031896001.1
    Accession Version XM_031896001.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2094bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(XXV) chain isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGTTGGGAA CAGGAGAGAT GAGAAAGAAT GGAGAGAAGT CCCAGGGATG TCACAGGGAC 
    AAGCTTGGAG GGGTGCTCAG TGGCTGGGTT AGGACTTTGC CCAGTGTCTT GGCATTTGTG
    TTCTCCCTGT TGTCCATGGC ATCATTTCTT TATCTGAATA TGAAGACCAA TGACCTGCAG
    GGAAGGGTTT GGGCACTGCA GCATGGCAAG TCTGGGGACA TTGCTTTCCA TATGCCAGGA
    TTATCTGTGG ATCAGCTTAA CTCCATCGTC CAGGAAAAAG TGGATCGCCT CTTGTCCCAG
    AGGTCCTATG AACACATGGC AAAGCTCCGA ATCGCAAGAG ATGCACCGGC AGACTGCAAC
    TGCCCAGCAG GCCCACCTGG ACAGCGAGGA AAAAGGGGGA GAAGAGGTGA ACCTGGACCA
    CCAGGTCCTC CTGGCCCAAA GGGAGACAAG GGTGATCAAG GTGATCAGGG ACCTCGGATG
    GTGTTTCCTA AGATCAATCA TGGCTTTCTC TCTGCTGATC AGCAGCTAAT AAAACGCCGC
    CTGATTAAGG GTGATCAAGG ACAAGCAGGC CCCCCGGGCC CCCCAGGTCC ACCGGGTCCA
    CCAGGTCCTA GGGGTCCTCC AGGAGACACT GGGAAAGATG GTCCAAGGGG ATTGTCTGGA
    GCTCCAGGGG AGACAGGGAA ACCAGGCGAA CAAGGCTTAA TGGGTCCAAT GGGGCCTCCT
    GGACAAAAGG GGTCCATTGG TGCACCTGGA ATTCCTGGAG TAGATGGTCA AAAGGGCGAG
    CAAGGAGAAG TTGGGACACC TGGCATTGAT GGAGTATCTG GAATGAAGGG TGATCCAGGT
    GAACAAGGAG ATAAGGGTGA TCCAGGGGAA GGCGGCCCTA AAGGAGATAT AGGAGCTAAG
    GGGGATCCAG GATCTTCTGC TGCGGGAATA AAGGGAGAGC CTGGAGAGCC TGGACGTTTT
    GGGCAGAAGG GTGAACCAGG GCTTCCTGGG CTTCCTGGAC TTCCTGGGAT TAAGGGTGAG
    CCAGGATTTC TAGGGCCTCA AGGAGAACCT GGATTACCAG GCTTACCAGG AACTAAGGGG
    GAACGAGGAG AAATTGGTCC ACCTGGAAGG GGAGAAAGAG GCAGTACTGG ACTAACTGGA
    CCAAAGGGGG AGCCTGGTGA ATCCGGATCT AGAGGCCCAA AGGGGTCAAA GGGAGAATCT
    GGAAGCAAAG GAGATTCTGG CCCCATTGGT CCTAGGGGGC CTCCAGGTCA AAAAGGGGAT
    CAAGGGGCTA CTGAGATCAT AGATTACAAT GGGAATATTC AGGAAGCCTT GCAGGGGCCG
    CCTGGACCTC CTGGACCACC TGGTTTGCCA GGTCCAAAGG GTGATGGAGG TCCTGTTGGT
    CCTCCAGGAG TTGATGGGGA GCAGGGTCCA AAAGGATCAA AGGGTGACCA TGGTGATTCC
    GGGACATCTG GAGAGAAGGG AGGAATTGGT CTCCCTGGTT TGCCGGGTGC AAATGGAATG
    AAAGGAGAGA AAGGAGATCA TGGTTCCCCT GGGCCCCATG GCGCTTCTAT CATTGGCCCC
    CCTGGACCTC CAGGGCCACA TGGCCCCCCT GGACCCATGG GACCACATGG ACTTACAGGG
    CCAAAGGGTG AACCAGGGGT AAATGGTCTT AAAGGATTGA GGGGTGAGCC AGGTCAAAAG
    GGTGAAAGAG GACCACTGGG TCTTCCAGGA GCATCTGGCT TAGACGGAAA GCCAGGACCC
    AGGGGGAATG ATGGTCCCAT TGGTGCTTCT GGTCCAGCAG GTCCAAAAGG AGACAGAGGA
    GAAAAAGGAG TTGTTGGTGA TCCCGGACCC AGAGGGCCTT ATGGTTTACC TGGCAAAGAT
    GGAGAGCCTG GTCTAGACGG TTTTCCGGGT CCTCGTGGAG AAAAAGGTGA CCCTGGAGAA
    AGGGGAGAAA AGGGGGAAAA GGGAAAGAAA GGCAAAAAAG GGCCTAAAGG GGAGAAAGGA
    GAACAGGGTG CCCCTGGATT AGATGCACCT TGCCCATTGG GACCAGATGG GCTTCCAATG
    CCTGGTTGTT GGCAAAAGGG AGTGACCCCA TGGCTTGTTG CCAAAAAGAG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751861.1
    CDS480..2573
    Translation

    Target ORF information:

    RefSeq Version XM_031896001.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type XXV, alpha 1, gene 2 (col25a1.2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896001.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGTTGGGAA CAGGAGAGAT GAGAAAGAAT GGAGAGAAGT CCCAGGGATG TCACAGGGAC 
    AAGCTTGGAG GGGTGCTCAG TGGCTGGGTT AGGACTTTGC CCAGTGTCTT GGCATTTGTG
    TTCTCCCTGT TGTCCATGGC ATCATTTCTT TATCTGAATA TGAAGACCAA TGACCTGCAG
    GGAAGGGTTT GGGCACTGCA GCATGGCAAG TCTGGGGACA TTGCTTTCCA TATGCCAGGA
    TTATCTGTGG ATCAGCTTAA CTCCATCGTC CAGGAAAAAG TGGATCGCCT CTTGTCCCAG
    AGGTCCTATG AACACATGGC AAAGCTCCGA ATCGCAAGAG ATGCACCGGC AGACTGCAAC
    TGCCCAGCAG GCCCACCTGG ACAGCGAGGA AAAAGGGGGA GAAGAGGTGA ACCTGGACCA
    CCAGGTCCTC CTGGCCCAAA GGGAGACAAG GGTGATCAAG GTGATCAGGG ACCTCGGATG
    GTGTTTCCTA AGATCAATCA TGGCTTTCTC TCTGCTGATC AGCAGCTAAT AAAACGCCGC
    CTGATTAAGG GTGATCAAGG ACAAGCAGGC CCCCCGGGCC CCCCAGGTCC ACCGGGTCCA
    CCAGGTCCTA GGGGTCCTCC AGGAGACACT GGGAAAGATG GTCCAAGGGG ATTGTCTGGA
    GCTCCAGGGG AGACAGGGAA ACCAGGCGAA CAAGGCTTAA TGGGTCCAAT GGGGCCTCCT
    GGACAAAAGG GGTCCATTGG TGCACCTGGA ATTCCTGGAG TAGATGGTCA AAAGGGCGAG
    CAAGGAGAAG TTGGGACACC TGGCATTGAT GGAGTATCTG GAATGAAGGG TGATCCAGGT
    GAACAAGGAG ATAAGGGTGA TCCAGGGGAA GGCGGCCCTA AAGGAGATAT AGGAGCTAAG
    GGGGATCCAG GATCTTCTGC TGCGGGAATA AAGGGAGAGC CTGGAGAGCC TGGACGTTTT
    GGGCAGAAGG GTGAACCAGG GCTTCCTGGG CTTCCTGGAC TTCCTGGGAT TAAGGGTGAG
    CCAGGATTTC TAGGGCCTCA AGGAGAACCT GGATTACCAG GCTTACCAGG AACTAAGGGG
    GAACGAGGAG AAATTGGTCC ACCTGGAAGG GGAGAAAGAG GCAGTACTGG ACTAACTGGA
    CCAAAGGGGG AGCCTGGTGA ATCCGGATCT AGAGGCCCAA AGGGGTCAAA GGGAGAATCT
    GGAAGCAAAG GAGATTCTGG CCCCATTGGT CCTAGGGGGC CTCCAGGTCA AAAAGGGGAT
    CAAGGGGCTA CTGAGATCAT AGATTACAAT GGGAATATTC AGGAAGCCTT GCAGGGGCCG
    CCTGGACCTC CTGGACCACC TGGTTTGCCA GGTCCAAAGG GTGATGGAGG TCCTGTTGGT
    CCTCCAGGAG TTGATGGGGA GCAGGGTCCA AAAGGATCAA AGGGTGACCA TGGTGATTCC
    GGGACATCTG GAGAGAAGGG AGGAATTGGT CTCCCTGGTT TGCCGGGTGC AAATGGAATG
    AAAGGAGAGA AAGGAGATCA TGGTTCCCCT GGGCCCCATG GCGCTTCTAT CATTGGCCCC
    CCTGGACCTC CAGGGCCACA TGGCCCCCCT GGACCCATGG GACCACATGG ACTTACAGGG
    CCAAAGGGTG AACCAGGGGT AAATGGTCTT AAAGGATTGA GGGGTGAGCC AGGTCAAAAG
    GGTGAAAGAG GACCACTGGG TCTTCCAGGA GCATCTGGCT TAGACGGAAA GCCAGGACCC
    AGGGGGAATG ATGGTCCCAT TGGTGCTTCT GGTCCAGCAG GTCCAAAAGG AGACAGAGGA
    GAAAAAGGAG TTGTTGGTGA TCCCGGACCC AGAGGGCCTT ATGGTTTACC TGGCAAAGAT
    GGAGAGCCTG GTCTAGACGG TTTTCCGGGT CCTCGTGGAG AAAAAGGTGA CCCTGGAGAA
    AGGGGAGAAA AGGGGGAAAA GGGAAAGAAA GGCAAAAAAG GGCCTAAAGG GGAGAAAGGA
    GAACAGGGTG CCCCTGGATT AGATGCACCT TGCCCATTGG GACCAGATGG GCTTCCAATG
    CCTGGTTGTT GGCAAAAGGG AGTGACCCCA TGGCTTGTTG CCAAAAAGAG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47630
    Clone ID Related Accession (Same CDS sequence) XM_031896002.1
    Accession Version XM_031896002.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2085bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(XXV) chain isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGTTGGGAA CAGGAGAGAT GAGAAAGAAT GGAGAGAAGT CCCAGGGATG TCACAGGGAC 
    AAGCTTGGAG GGGTGCTCAG TGGCTGGGTT AGGACTTTGC CCAGTGTCTT GGCATTTGTG
    TTCTCCCTGT TGTCCATGGC ATCATTTCTT TATCTGAATA TGAAGACCAA TGACCTGCAG
    GGAAGGGTTT GGGCACTGCA GCATGGCAAG TCTGGGGACA TTGCTTTCCA TATGCCAGGA
    TTATCTGTGG ATCAGCTTAA CTCCATCGTC CAGGAAAAAG TGGATCGCCT CTTGTCCCAG
    AGGTCCTATG AACACATGGC AAAGCTCCGA ATCGCAAGAG ATGCACCGGC AGACTGCAAC
    TGCCCAGCAG GCCCACCTGG ACAGCGAGGA AAAAGGGGGA GAAGAGGTGA ACCTGGACCA
    CCAGGTCCTC CTGGCCCAAA GGGAGACAAG GGTGATCAAG GTGATCAGGG ACCTCGGATG
    GTGTTTCCTA AGATCAATCA TGGCTTTCTC TCTGCTGATC AGCAGCTAAT AAAACGCCGC
    CTGATTAAGG GTGATCAAGG ACAAGCAGGC CCCCCGGGCC CCCCAGGTCC ACCGGGTCCA
    CCAGGTCCTA GGGGTCCTCC AGGAGACACT GGGAAAGATG GTCCAAGGGG ATTGTCTGGA
    GCTCCAGGGG AGACAGGGAA ACCAGGCGAA CAAGGCTTAA TGGGTCCAAT GGGGCCTCCT
    GGACAAAAGG GGTCCATTGG TGCACCTGGA ATTCCTGGAG TAGATGGTCA AAAGGGCGAG
    CAAGGAGAAG TTGGGACACC TGGCATTGAT GGAGTATCTG GAATGAAGGG TGATCCAGGT
    GAACAAGGAG ATAAGGGTGA TCCAGGGGAA GGCGGCCCTA AAGGAGATAT AGGAGCTAAG
    GGGGATCCAG GATCTTCTGC TGCGGGAATA AAGGGAGAGC CTGGAGAGCC TGGACGTTTT
    GGGCAGAAGG GTGAACCAGG GCTTCCTGGG CTTCCTGGAC TTCCTGGGAT TAAGGGTGAG
    CCAGGATTTC TAGGGCCTCA AGGAGAACCT GGATTACCAG GCTTACCAGG AACTAAGGGG
    GAACGAGGAG AAATTGGTCC ACCTGGAAGG GGAGAAAGAG GCAGTACTGG ACTAACTGGA
    CCAAAGGGGG AGCCTGGTGA ATCCGGATCT AGAGGCCCAA AGGGGTCAAA GGGAGAATCT
    GGAAGCAAAG GAGATTCTGG CCCCATTGGT CCTAGGGGGC CTCCAGGTCA AAAAGGGGAT
    CAAGGGGCTA CTGAGATCAT AGATTACAAT GGGAATATTC AGGAAGCCTT GCAGAGGATT
    ACAGCCATTA CTGTTACGGG GCCGCCTGGA CCTCCTGGAC CACCTGGTTT GCCAGGTCCA
    AAGGGTGATG GAGGTCCTGT TGGTCCTCCA GGAGTTGATG GGGAGCAGGG TCCAAAAGGA
    TCAAAGGGTG ACCATGGTGA TTCCGGGACA TCTGGAGAGA AGGGAGGAAT TGGTCTCCCT
    GGTTTGCCGG GTGCAAATGG AATGAAAGGA GAGAAAGGAG ATCATGGTTC CCCTGGGCCC
    CATGGCGCTT CTATCATTGG CCCCCCTGGA CCTCCAGGGC CACATGGCCC CCCTGGACCC
    ATGGGACCAC ATGGACTTAC AGGGCCAAAG GGTGAACCAG GGGTAAATGG TCTTAAAGGA
    TTGAGGGGTG AGCCAGGTCA AAAGGGTGAA AGAGGACCAC TGGGTCTTCC AGGAGCATCT
    GGCTTAGACG GAAAGCCAGG ACCCAGGGGG AATGATGGTC CCATTGGTGC TTCTGGTCCA
    GCAGGTCCAA AAGGAGACAG AGGAGAAAAA GGAGTTGTTG GTGATCCCGG ACCCAGAGGG
    CCTTATGGTT TACCTGGCAA AGATGGAGAG CCTGGTCTAG ACGGTTTTCC GGGTCCTCGT
    GGAGAAAAAG GTGACCCTGG AGAAAGGGGA GAAAAGGGGG AAAAGGGAAA GAAAGGCAAA
    AAAGGGCCTA AAGGGGAGAA AGGAGAACAG GGTGCCCCTG GATTAGATGC ACCTTGCCCA
    TTGGGACCAG ATGGGCTTCC AATGCCTGGT TGTTGGCAAA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751862.1
    CDS480..2564
    Translation

    Target ORF information:

    RefSeq Version XM_031896002.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type XXV, alpha 1, gene 2 (col25a1.2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896002.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGTTGGGAA CAGGAGAGAT GAGAAAGAAT GGAGAGAAGT CCCAGGGATG TCACAGGGAC 
    AAGCTTGGAG GGGTGCTCAG TGGCTGGGTT AGGACTTTGC CCAGTGTCTT GGCATTTGTG
    TTCTCCCTGT TGTCCATGGC ATCATTTCTT TATCTGAATA TGAAGACCAA TGACCTGCAG
    GGAAGGGTTT GGGCACTGCA GCATGGCAAG TCTGGGGACA TTGCTTTCCA TATGCCAGGA
    TTATCTGTGG ATCAGCTTAA CTCCATCGTC CAGGAAAAAG TGGATCGCCT CTTGTCCCAG
    AGGTCCTATG AACACATGGC AAAGCTCCGA ATCGCAAGAG ATGCACCGGC AGACTGCAAC
    TGCCCAGCAG GCCCACCTGG ACAGCGAGGA AAAAGGGGGA GAAGAGGTGA ACCTGGACCA
    CCAGGTCCTC CTGGCCCAAA GGGAGACAAG GGTGATCAAG GTGATCAGGG ACCTCGGATG
    GTGTTTCCTA AGATCAATCA TGGCTTTCTC TCTGCTGATC AGCAGCTAAT AAAACGCCGC
    CTGATTAAGG GTGATCAAGG ACAAGCAGGC CCCCCGGGCC CCCCAGGTCC ACCGGGTCCA
    CCAGGTCCTA GGGGTCCTCC AGGAGACACT GGGAAAGATG GTCCAAGGGG ATTGTCTGGA
    GCTCCAGGGG AGACAGGGAA ACCAGGCGAA CAAGGCTTAA TGGGTCCAAT GGGGCCTCCT
    GGACAAAAGG GGTCCATTGG TGCACCTGGA ATTCCTGGAG TAGATGGTCA AAAGGGCGAG
    CAAGGAGAAG TTGGGACACC TGGCATTGAT GGAGTATCTG GAATGAAGGG TGATCCAGGT
    GAACAAGGAG ATAAGGGTGA TCCAGGGGAA GGCGGCCCTA AAGGAGATAT AGGAGCTAAG
    GGGGATCCAG GATCTTCTGC TGCGGGAATA AAGGGAGAGC CTGGAGAGCC TGGACGTTTT
    GGGCAGAAGG GTGAACCAGG GCTTCCTGGG CTTCCTGGAC TTCCTGGGAT TAAGGGTGAG
    CCAGGATTTC TAGGGCCTCA AGGAGAACCT GGATTACCAG GCTTACCAGG AACTAAGGGG
    GAACGAGGAG AAATTGGTCC ACCTGGAAGG GGAGAAAGAG GCAGTACTGG ACTAACTGGA
    CCAAAGGGGG AGCCTGGTGA ATCCGGATCT AGAGGCCCAA AGGGGTCAAA GGGAGAATCT
    GGAAGCAAAG GAGATTCTGG CCCCATTGGT CCTAGGGGGC CTCCAGGTCA AAAAGGGGAT
    CAAGGGGCTA CTGAGATCAT AGATTACAAT GGGAATATTC AGGAAGCCTT GCAGAGGATT
    ACAGCCATTA CTGTTACGGG GCCGCCTGGA CCTCCTGGAC CACCTGGTTT GCCAGGTCCA
    AAGGGTGATG GAGGTCCTGT TGGTCCTCCA GGAGTTGATG GGGAGCAGGG TCCAAAAGGA
    TCAAAGGGTG ACCATGGTGA TTCCGGGACA TCTGGAGAGA AGGGAGGAAT TGGTCTCCCT
    GGTTTGCCGG GTGCAAATGG AATGAAAGGA GAGAAAGGAG ATCATGGTTC CCCTGGGCCC
    CATGGCGCTT CTATCATTGG CCCCCCTGGA CCTCCAGGGC CACATGGCCC CCCTGGACCC
    ATGGGACCAC ATGGACTTAC AGGGCCAAAG GGTGAACCAG GGGTAAATGG TCTTAAAGGA
    TTGAGGGGTG AGCCAGGTCA AAAGGGTGAA AGAGGACCAC TGGGTCTTCC AGGAGCATCT
    GGCTTAGACG GAAAGCCAGG ACCCAGGGGG AATGATGGTC CCATTGGTGC TTCTGGTCCA
    GCAGGTCCAA AAGGAGACAG AGGAGAAAAA GGAGTTGTTG GTGATCCCGG ACCCAGAGGG
    CCTTATGGTT TACCTGGCAA AGATGGAGAG CCTGGTCTAG ACGGTTTTCC GGGTCCTCGT
    GGAGAAAAAG GTGACCCTGG AGAAAGGGGA GAAAAGGGGG AAAAGGGAAA GAAAGGCAAA
    AAAGGGCCTA AAGGGGAGAA AGGAGAACAG GGTGCCCCTG GATTAGATGC ACCTTGCCCA
    TTGGGACCAG ATGGGCTTCC AATGCCTGGT TGTTGGCAAA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47631
    Clone ID Related Accession (Same CDS sequence) XM_031896004.1
    Accession Version XM_031896004.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2082bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(XXV) chain isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGTTGGGAA CAGGAGAGAT GAGAAAGAAT GGAGAGAAGT CCCAGGGATG TCACAGGGAC 
    AAGCTTGGAG GGGTGCTCAG TGGCTGGGTT AGGACTTTGC CCAGTGTCTT GGCATTTGTG
    TTCTCCCTGT TGTCCATGGC ATCATTTCTT TATCTGAATA TGAAGACCAA TGACCTGCAG
    GGAAGGGTTT GGGCACTGCA GCATGGCAAG TCTGGGGACA TTGCTTTCCA TATGCCAGGA
    TTATCTGTGG ATCAGCTTAA CTCCATCGTC CAGGAAAAAG TGGATCGCCT CTTGTCCCAG
    AGGTCCTATG AACACATGGC AAAGCTCCGA ATCGCAAGAG ATGCACCGGC AGACTGCAAC
    TGCCCAGCAG GCCCACCTGG ACAGCGAGGA AAAAGGGGGA GAAGAGGTGA ACCTGGACCA
    CCAGGTCCTC CTGGCCCAAA GGGAGACAAG GGTGATCAAG GTGATCAGGG ACCTCGGATG
    GTGTTTCCTA AGATCAATCA TGGCTTTCTC TCTGCTGATC AGCAGCTAAT AAAACGCCGC
    CTGATTAAGG GTGATCAAGG ACAAGCAGGC CCCCCGGGCC CCCCAGGTCC ACCGGGTCCA
    CCAGGTCCTA GGGGTCCTCC AGGAGACACT GGGAAAGATG GTCCAAGGGG ATTGTCTGGA
    GCTCCAGGGG AGACAGGGAA ACCAGGCGAA CAAGGCTTAA TGGGTCCAAT GGGGCCTCCT
    GGACAAAAGG GGTCCATTGG TGCACCTGGA ATTCCTGGAG TAGATGGTCA AAAGGGCGAG
    CAAGGAGAAG TTGGGACACC TGGCATTGAT GGAGTATCTG GAATGAAGGG TGATCCAGGT
    GAACAAGGAG ATAAGGGTGA TCCAGGGGAA GGCGGCCCTA AAGGAGATAT AGGAGCTAAG
    GGGGATCCAG GATCTTCTGC TGCGGGAATA AAGGGAGAGC CTGGAGAGCC TGGACGTTTT
    GGGCAGAAGG GTGAACCAGG GCTTCCTGGG CTTCCTGGAC TTCCTGGGAT TAAGGGTGAG
    CCAGGATTTC TAGGGCCTCA AGGAGAACCT GGATTACCAG GCTTACCAGG AACTAAGGGG
    GAACGAGGAG AAATTGGTCC ACCTGGAAGG GGAGAAAGAG GCAGTACTGG ACTAACTGGA
    CCAAAGGGGG AGCCTGGTGA ATCCGGATCT AGAGGCCCAA AGGGGTCAAA GGGAGAATCT
    GGAAGCAAAG GAGATTCTGG CCCCATTGGT CCTAGGGGGC CTCCAGGTCA AAAAGGGGAT
    CAAGGGGCTA CTGAGATCAT AGATTACAAT GGGAATATTC AGGAAGCCTT GCAGAGGATT
    ACAGCCATTA CTGTTACGGG GCCGCCTGGA CCTCCTGGAC CACCTGGTTT GCCAGGTCCA
    AAGGGTGATG GAGGTCCTGT TGGTCCTCCA GGAGTTGATG GGGAGCAGGG TCCAAAAGGA
    TCAAAGGGTG ACCATGGTGA TTCCGGGACA TCTGGAGAGA AGGGAGGAAT TGGTCTCCCT
    GGTTTGCCGG GTGCAAATGG AATGAAAGGA GAGAAAGGAG ATCATGGTTC CCCTGGGCCC
    CATGGCGCTT CTATCATTGG CCCCCCTGGA CCTCCAGGGC CACATGGCCC CCCTGGACCC
    ATGGGACCAC ATGGACTTAC AGGGCCAAAG GGTGAACCAG GGGTAAATGG TCTTAAAGGA
    TTGAGGGGTG AGCCAGGTCA AAAGGGTGAA AGAGGACCAC TGGGTCTTCC AGGGAATGAT
    GGTCCCATTG GTGCTTCTGG TCCAGCAGGT CCAAAAGGAG ACAGAGGAGA AAAAGGAGTT
    GTTGGTGATC CCGGACCCAG AGGGCCTTAT GGTTTACCTG GCAAAGATGG AGAGCCTGGT
    CTAGACGGTT TTCCGGGTCC TCGTGGAGAA AAAGGTGACC CTGGAGAAAG GGGAGAAAAG
    GGGGAAAAGG GAAAGAAAGG CAAAAAAGGG CCTAAAGGGG AGAAAGGAGA ACAGGGTGCC
    CCTGGATTAG ATGCACCTTG CCCATTGGGA CCAGATGGGC TTCCAATGCC TGGTTGTTGG
    CAAAAGGGAG TGACCCCATG GCTTGTTGCC AAAAAGAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751864.1
    CDS480..2561
    Translation

    Target ORF information:

    RefSeq Version XM_031896004.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type XXV, alpha 1, gene 2 (col25a1.2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896004.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGTTGGGAA CAGGAGAGAT GAGAAAGAAT GGAGAGAAGT CCCAGGGATG TCACAGGGAC 
    AAGCTTGGAG GGGTGCTCAG TGGCTGGGTT AGGACTTTGC CCAGTGTCTT GGCATTTGTG
    TTCTCCCTGT TGTCCATGGC ATCATTTCTT TATCTGAATA TGAAGACCAA TGACCTGCAG
    GGAAGGGTTT GGGCACTGCA GCATGGCAAG TCTGGGGACA TTGCTTTCCA TATGCCAGGA
    TTATCTGTGG ATCAGCTTAA CTCCATCGTC CAGGAAAAAG TGGATCGCCT CTTGTCCCAG
    AGGTCCTATG AACACATGGC AAAGCTCCGA ATCGCAAGAG ATGCACCGGC AGACTGCAAC
    TGCCCAGCAG GCCCACCTGG ACAGCGAGGA AAAAGGGGGA GAAGAGGTGA ACCTGGACCA
    CCAGGTCCTC CTGGCCCAAA GGGAGACAAG GGTGATCAAG GTGATCAGGG ACCTCGGATG
    GTGTTTCCTA AGATCAATCA TGGCTTTCTC TCTGCTGATC AGCAGCTAAT AAAACGCCGC
    CTGATTAAGG GTGATCAAGG ACAAGCAGGC CCCCCGGGCC CCCCAGGTCC ACCGGGTCCA
    CCAGGTCCTA GGGGTCCTCC AGGAGACACT GGGAAAGATG GTCCAAGGGG ATTGTCTGGA
    GCTCCAGGGG AGACAGGGAA ACCAGGCGAA CAAGGCTTAA TGGGTCCAAT GGGGCCTCCT
    GGACAAAAGG GGTCCATTGG TGCACCTGGA ATTCCTGGAG TAGATGGTCA AAAGGGCGAG
    CAAGGAGAAG TTGGGACACC TGGCATTGAT GGAGTATCTG GAATGAAGGG TGATCCAGGT
    GAACAAGGAG ATAAGGGTGA TCCAGGGGAA GGCGGCCCTA AAGGAGATAT AGGAGCTAAG
    GGGGATCCAG GATCTTCTGC TGCGGGAATA AAGGGAGAGC CTGGAGAGCC TGGACGTTTT
    GGGCAGAAGG GTGAACCAGG GCTTCCTGGG CTTCCTGGAC TTCCTGGGAT TAAGGGTGAG
    CCAGGATTTC TAGGGCCTCA AGGAGAACCT GGATTACCAG GCTTACCAGG AACTAAGGGG
    GAACGAGGAG AAATTGGTCC ACCTGGAAGG GGAGAAAGAG GCAGTACTGG ACTAACTGGA
    CCAAAGGGGG AGCCTGGTGA ATCCGGATCT AGAGGCCCAA AGGGGTCAAA GGGAGAATCT
    GGAAGCAAAG GAGATTCTGG CCCCATTGGT CCTAGGGGGC CTCCAGGTCA AAAAGGGGAT
    CAAGGGGCTA CTGAGATCAT AGATTACAAT GGGAATATTC AGGAAGCCTT GCAGAGGATT
    ACAGCCATTA CTGTTACGGG GCCGCCTGGA CCTCCTGGAC CACCTGGTTT GCCAGGTCCA
    AAGGGTGATG GAGGTCCTGT TGGTCCTCCA GGAGTTGATG GGGAGCAGGG TCCAAAAGGA
    TCAAAGGGTG ACCATGGTGA TTCCGGGACA TCTGGAGAGA AGGGAGGAAT TGGTCTCCCT
    GGTTTGCCGG GTGCAAATGG AATGAAAGGA GAGAAAGGAG ATCATGGTTC CCCTGGGCCC
    CATGGCGCTT CTATCATTGG CCCCCCTGGA CCTCCAGGGC CACATGGCCC CCCTGGACCC
    ATGGGACCAC ATGGACTTAC AGGGCCAAAG GGTGAACCAG GGGTAAATGG TCTTAAAGGA
    TTGAGGGGTG AGCCAGGTCA AAAGGGTGAA AGAGGACCAC TGGGTCTTCC AGGGAATGAT
    GGTCCCATTG GTGCTTCTGG TCCAGCAGGT CCAAAAGGAG ACAGAGGAGA AAAAGGAGTT
    GTTGGTGATC CCGGACCCAG AGGGCCTTAT GGTTTACCTG GCAAAGATGG AGAGCCTGGT
    CTAGACGGTT TTCCGGGTCC TCGTGGAGAA AAAGGTGACC CTGGAGAAAG GGGAGAAAAG
    GGGGAAAAGG GAAAGAAAGG CAAAAAAGGG CCTAAAGGGG AGAAAGGAGA ACAGGGTGCC
    CCTGGATTAG ATGCACCTTG CCCATTGGGA CCAGATGGGC TTCCAATGCC TGGTTGTTGG
    CAAAAGGGAG TGACCCCATG GCTTGTTGCC AAAAAGAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47632
    Clone ID Related Accession (Same CDS sequence) XM_031896006.1
    Accession Version XM_031896006.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2076bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(XXV) chain isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGTTGGGAA CAGGAGAGAT GAGAAAGAAT GGAGAGAAGT CCCAGGGATG TCACAGGGAC 
    AAGCTTGGAG GGGTGCTCAG TGGCTGGGTT AGGACTTTGC CCAGTGTCTT GGCATTTGTG
    TTCTCCCTGT TGTCCATGGC ATCATTTCTT TATCTGAATA TGAAGACCAA TGACCTGCAG
    GGAAGGGTTT GGGCACTGCA GCATGGCAAG TCTGGGGACA TTGCTTTCCA TATGCCAGGA
    TTATCTGTGG ATCAGCTTAA CTCCATCGTC CAGGAAAAAG TGGATCGCCT CTTGTCCCAG
    AGGTCCTATG AACACATGGC AAAGCTCCGA ATCGCAAGAG ATGCACCGGC AGACTGCAAC
    TGCCCAGCAG GCCCACCTGG ACAGCGAGGA AAAAGGGGGA GAAGAGGTGA ACCTGGACCA
    CCAGGTCCTC CTGGCCCAAA GGGAGACAAG GGTGATCAAG GTGATCAGGG ACCTCGGATG
    GTGTTTCCTA AGATCAATCA TGGCTTTCTC TCTGCTGATC AGCAGCTAAT AAAACGCCGC
    CTGATTAAGG GTGATCAAGG ACAAGCAGGC CCCCCGGGCC CCCCAGGTCC ACCGGGTCCA
    CCAGGTCCTA GGGGTCCTCC AGGAGACACT GGGAAAGATG GTCCAAGGGG ATTGTCTGGA
    GCTCCAGGGG AGACAGGGAA ACCAGGCGAA CAAGGCTTAA TGGGTCCAAT GGGGCCTCCT
    GGACAAAAGG GGTCCATTGG TGCACCTGGA ATTCCTGGAG TAGATGGTCA AAAGGGCGAG
    CAAGGAGAAG TTGGGACACC TGGCATTGAT GGAGTATCTG GAATGAAGGG TGATCCAGGT
    GAACAAGGAG ATAAGGGTGA TCCAGGGGAA GGCGGCCCTA AAGGAGATAT AGGAGCTAAG
    GGGGATCCAG GATCTTCTGC TGCGGGAATA AAGGGAGAGC CTGGAGAGCC TGGACGTTTT
    GGGCAGAAGG GTGAACCAGG GCTTCCTGGG CTTCCTGGAC TTCCTGGGAT TAAGGGTGAG
    CCAGGATTTC TAGGGCCTCA AGGAGAACCT GGATTACCAG GCTTACCAGG AACTAAGGGG
    GAACGAGGAG AAATTGGTCC ACCTGGAAGG GGAGAAAGAG GCAGTACTGG ACTAACTGGA
    CCAAAGGGGG AGCCTGGTGA ATCCGGATCT AGAGGCCCAA AGGGGTCAAA GGGAGAATCT
    GGAAGCAAAG GAGATTCTGG CCCCATTGGT CCTAGGGGGC CTCCAGGTCA AAAAGGGGAT
    CAAGGGGCTA CTGAGATCAT AGATTACAAT GGGAATATTC AGGAAGCCTT GCAGAGGATT
    ACAGCCATTA CTGTTACGGG GCCGCCTGGA CCTCCTGGAC CACCTGGTTT GCCAGGTCCA
    AAGGGTGATG GAGGTCCTGT TGGTCCTCCA GGAGTTGATG GGGAGCAGGG TCCAAAAGGA
    TCAAAGGGTG ACCATGGTGA TTCCGGGACA TCTGGAGAGA AGGGAGGAAT TGGTCTCCCT
    GGTTTGCCGG GTGCAAATGG AATGAAAGGA GAGAAAGGAG ATCATGGTTC CCCTGGGCCC
    CATGGCGCTT CTATCATTGG CCCCCCTGGA CCTCCAGGGC CACATGGCCC CCCTGGACCC
    ATGGGACCAC ATGGACTTAC AGGGCCAAAG GGTGAACCAG GGGTAAATGG TCTTAAAGGA
    TTGAGGGGTG AGCCAGGTCA AAAGGGTGAA AGAGGACCAC TGGGTCTTCC AGGAGCATCT
    GGCTTAGACG GAAAGCCAGG ACCCAGGGGG AATGATGGTC CCATTGGTGC TTCTGGTCCA
    GCAGGTCCAA AAGGAGACAG AGGAGAAAAA GGAGTTGTTG GTGATCCCGG ACCCAGAGGG
    CCTTATGGTT TACCTGGCAA AGATGGAGAG CCTGGTCTAG ACGGTTTTCC GGGTCCTCGT
    GGAGAAAAAG GTGACCCTGG AGAAAGGGGA GAAAAGGGTT TCCGTGGATT AAAGGGGGAA
    AAAGGGGAAC CAGGTCAGCC TGGCCTGGAT GGGCTGGATG CCCCTTGCCA ATTGGGACCA
    GATGGGCTTC CAATGCCTGG TTGTTGGCAA AAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751866.1
    CDS480..2555
    Translation

    Target ORF information:

    RefSeq Version XM_031896006.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type XXV, alpha 1, gene 2 (col25a1.2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896006.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGTTGGGAA CAGGAGAGAT GAGAAAGAAT GGAGAGAAGT CCCAGGGATG TCACAGGGAC 
    AAGCTTGGAG GGGTGCTCAG TGGCTGGGTT AGGACTTTGC CCAGTGTCTT GGCATTTGTG
    TTCTCCCTGT TGTCCATGGC ATCATTTCTT TATCTGAATA TGAAGACCAA TGACCTGCAG
    GGAAGGGTTT GGGCACTGCA GCATGGCAAG TCTGGGGACA TTGCTTTCCA TATGCCAGGA
    TTATCTGTGG ATCAGCTTAA CTCCATCGTC CAGGAAAAAG TGGATCGCCT CTTGTCCCAG
    AGGTCCTATG AACACATGGC AAAGCTCCGA ATCGCAAGAG ATGCACCGGC AGACTGCAAC
    TGCCCAGCAG GCCCACCTGG ACAGCGAGGA AAAAGGGGGA GAAGAGGTGA ACCTGGACCA
    CCAGGTCCTC CTGGCCCAAA GGGAGACAAG GGTGATCAAG GTGATCAGGG ACCTCGGATG
    GTGTTTCCTA AGATCAATCA TGGCTTTCTC TCTGCTGATC AGCAGCTAAT AAAACGCCGC
    CTGATTAAGG GTGATCAAGG ACAAGCAGGC CCCCCGGGCC CCCCAGGTCC ACCGGGTCCA
    CCAGGTCCTA GGGGTCCTCC AGGAGACACT GGGAAAGATG GTCCAAGGGG ATTGTCTGGA
    GCTCCAGGGG AGACAGGGAA ACCAGGCGAA CAAGGCTTAA TGGGTCCAAT GGGGCCTCCT
    GGACAAAAGG GGTCCATTGG TGCACCTGGA ATTCCTGGAG TAGATGGTCA AAAGGGCGAG
    CAAGGAGAAG TTGGGACACC TGGCATTGAT GGAGTATCTG GAATGAAGGG TGATCCAGGT
    GAACAAGGAG ATAAGGGTGA TCCAGGGGAA GGCGGCCCTA AAGGAGATAT AGGAGCTAAG
    GGGGATCCAG GATCTTCTGC TGCGGGAATA AAGGGAGAGC CTGGAGAGCC TGGACGTTTT
    GGGCAGAAGG GTGAACCAGG GCTTCCTGGG CTTCCTGGAC TTCCTGGGAT TAAGGGTGAG
    CCAGGATTTC TAGGGCCTCA AGGAGAACCT GGATTACCAG GCTTACCAGG AACTAAGGGG
    GAACGAGGAG AAATTGGTCC ACCTGGAAGG GGAGAAAGAG GCAGTACTGG ACTAACTGGA
    CCAAAGGGGG AGCCTGGTGA ATCCGGATCT AGAGGCCCAA AGGGGTCAAA GGGAGAATCT
    GGAAGCAAAG GAGATTCTGG CCCCATTGGT CCTAGGGGGC CTCCAGGTCA AAAAGGGGAT
    CAAGGGGCTA CTGAGATCAT AGATTACAAT GGGAATATTC AGGAAGCCTT GCAGAGGATT
    ACAGCCATTA CTGTTACGGG GCCGCCTGGA CCTCCTGGAC CACCTGGTTT GCCAGGTCCA
    AAGGGTGATG GAGGTCCTGT TGGTCCTCCA GGAGTTGATG GGGAGCAGGG TCCAAAAGGA
    TCAAAGGGTG ACCATGGTGA TTCCGGGACA TCTGGAGAGA AGGGAGGAAT TGGTCTCCCT
    GGTTTGCCGG GTGCAAATGG AATGAAAGGA GAGAAAGGAG ATCATGGTTC CCCTGGGCCC
    CATGGCGCTT CTATCATTGG CCCCCCTGGA CCTCCAGGGC CACATGGCCC CCCTGGACCC
    ATGGGACCAC ATGGACTTAC AGGGCCAAAG GGTGAACCAG GGGTAAATGG TCTTAAAGGA
    TTGAGGGGTG AGCCAGGTCA AAAGGGTGAA AGAGGACCAC TGGGTCTTCC AGGAGCATCT
    GGCTTAGACG GAAAGCCAGG ACCCAGGGGG AATGATGGTC CCATTGGTGC TTCTGGTCCA
    GCAGGTCCAA AAGGAGACAG AGGAGAAAAA GGAGTTGTTG GTGATCCCGG ACCCAGAGGG
    CCTTATGGTT TACCTGGCAA AGATGGAGAG CCTGGTCTAG ACGGTTTTCC GGGTCCTCGT
    GGAGAAAAAG GTGACCCTGG AGAAAGGGGA GAAAAGGGTT TCCGTGGATT AAAGGGGGAA
    AAAGGGGAAC CAGGTCAGCC TGGCCTGGAT GGGCTGGATG CCCCTTGCCA ATTGGGACCA
    GATGGGCTTC CAATGCCTGG TTGTTGGCAA AAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47633
    Clone ID Related Accession (Same CDS sequence) XM_031896008.1
    Accession Version XM_031896008.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2073bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(XXV) chain isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGTTGGGAA CAGGAGAGAT GAGAAAGAAT GGAGAGAAGT CCCAGGGATG TCACAGGGAC 
    AAGCTTGGAG GGGTGCTCAG TGGCTGGGTT AGGACTTTGC CCAGTGTCTT GGCATTTGTG
    TTCTCCCTGT TGTCCATGGC ATCATTTCTT TATCTGAATA TGAAGACCAA TGACCTGCAG
    GGAAGGGTTT GGGCACTGCA GCATGGCAAG TCTGGGGACA TTGCTTTCCA TATGCCAGGA
    TTATCTGTGG ATCAGCTTAA CTCCATCGTC CAGGAAAAAG TGGATCGCCT CTTGTCCCAG
    AGGTCCTATG AACACATGGC AAAGCTCCGA ATCGCAAGAG ATGCACCGGC AGACTGCAAC
    TGCCCAGCAG GCCCACCTGG ACAGCGAGGA AAAAGGGGGA GAAGAGGTGA ACCTGGACCA
    CCAGGTCCTC CTGGCCCAAA GGGAGACAAG GGTGATCAAG GTGATCAGGG ACCTCGGATG
    GTGTTTCCTA AGATCAATCA TGGCTTTCTC TCTGCTGATC AGCAGCTAAT AAAACGCCGC
    CTGATTAAGG GTGATCAAGG ACAAGCAGGC CCCCCGGGCC CCCCAGGTCC ACCGGGTCCA
    CCAGGTCCTA GGGGTCCTCC AGGAGACACT GGGAAAGATG GTCCAAGGGG ATTGTCTGGA
    GCTCCAGGGG AGACAGGGAA ACCAGGCGAA CAAGGCTTAA TGGGTCCAAT GGGGCCTCCT
    GGACAAAAGG GGTCCATTGG TGCACCTGGA ATTCCTGGAG TAGATGGTCA AAAGGGCGAG
    CAAGGAGAAG TTGGGACACC TGGCATTGAT GGAGTATCTG GAATGAAGGG TGATCCAGGT
    GAACAAGGAG ATAAGGGTGA TCCAGGGGAA GGCGGCCCTA AAGGAGATAT AGGAGCTAAG
    GGGGATCCAG GATCTTCTGC TGCGGGAATA AAGGGAGAGC CTGGAGAGCC TGGACGTTTT
    GGGCAGAAGG GTGAGCCAGG ATTTCTAGGG CCTCAAGGAG AACCTGGATT ACCAGGCTTA
    CCAGGAACTA AGGGGGAACG AGGAGAAATT GGTCCACCTG GAAGGGGAGA AAGAGGCAGT
    ACTGGACTAA CTGGACCAAA GGGGGAGCCT GGTGAATCCG GATCTAGAGG CCCAAAGGGG
    TCAAAGGGAG AATCTGGAAG CAAAGGAGAT TCTGGCCCCA TTGGTCCTAG GGGGCCTCCA
    GGTCAAAAAG GGGATCAAGG GGCTACTGAG ATCATAGATT ACAATGGGAA TATTCAGGAA
    GCCTTGCAGA GGATTACAGC CATTACTGTT ACGGGGCCGC CTGGACCTCC TGGACCACCT
    GGTTTGCCAG GTCCAAAGGG TGATGGAGGT CCTGTTGGTC CTCCAGGAGT TGATGGGGAG
    CAGGGTCCAA AAGGATCAAA GGGTGACCAT GGTGATTCCG GGACATCTGG AGAGAAGGGA
    GGAATTGGTC TCCCTGGTTT GCCGGGTGCA AATGGAATGA AAGGAGAGAA AGGAGATCAT
    GGTTCCCCTG GGCCCCATGG CGCTTCTATC ATTGGCCCCC CTGGACCTCC AGGGCCACAT
    GGCCCCCCTG GACCCATGGG ACCACATGGA CTTACAGGGC CAAAGGGTGA ACCAGGGGTA
    AATGGTCTTA AAGGATTGAG GGGTGAGCCA GGTCAAAAGG GTGAAAGAGG ACCACTGGGT
    CTTCCAGGAG CATCTGGCTT AGACGGAAAG CCAGGACCCA GGGGGAATGA TGGTCCCATT
    GGTGCTTCTG GTCCAGCAGG TCCAAAAGGA GACAGAGGAG AAAAAGGAGT TGTTGGTGAT
    CCCGGACCCA GAGGGCCTTA TGGTTTACCT GGCAAAGATG GAGAGCCTGG TCTAGACGGT
    TTTCCGGGTC CTCGTGGAGA AAAAGGTGAC CCTGGAGAAA GGGGAGAAAA GGGGGAAAAG
    GGAAAGAAAG GCAAAAAAGG GCCTAAAGGG GAGAAAGGAG AACAGGGTGC CCCTGGATTA
    GATGCACCTT GCCCATTGGG ACCAGATGGG CTTCCAATGC CTGGTTGTTG GCAAAAGGGA
    GTGACCCCAT GGCTTGTTGC CAAAAAGAGA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751868.1
    CDS480..2552
    Translation

    Target ORF information:

    RefSeq Version XM_031896008.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type XXV, alpha 1, gene 2 (col25a1.2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896008.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGTTGGGAA CAGGAGAGAT GAGAAAGAAT GGAGAGAAGT CCCAGGGATG TCACAGGGAC 
    AAGCTTGGAG GGGTGCTCAG TGGCTGGGTT AGGACTTTGC CCAGTGTCTT GGCATTTGTG
    TTCTCCCTGT TGTCCATGGC ATCATTTCTT TATCTGAATA TGAAGACCAA TGACCTGCAG
    GGAAGGGTTT GGGCACTGCA GCATGGCAAG TCTGGGGACA TTGCTTTCCA TATGCCAGGA
    TTATCTGTGG ATCAGCTTAA CTCCATCGTC CAGGAAAAAG TGGATCGCCT CTTGTCCCAG
    AGGTCCTATG AACACATGGC AAAGCTCCGA ATCGCAAGAG ATGCACCGGC AGACTGCAAC
    TGCCCAGCAG GCCCACCTGG ACAGCGAGGA AAAAGGGGGA GAAGAGGTGA ACCTGGACCA
    CCAGGTCCTC CTGGCCCAAA GGGAGACAAG GGTGATCAAG GTGATCAGGG ACCTCGGATG
    GTGTTTCCTA AGATCAATCA TGGCTTTCTC TCTGCTGATC AGCAGCTAAT AAAACGCCGC
    CTGATTAAGG GTGATCAAGG ACAAGCAGGC CCCCCGGGCC CCCCAGGTCC ACCGGGTCCA
    CCAGGTCCTA GGGGTCCTCC AGGAGACACT GGGAAAGATG GTCCAAGGGG ATTGTCTGGA
    GCTCCAGGGG AGACAGGGAA ACCAGGCGAA CAAGGCTTAA TGGGTCCAAT GGGGCCTCCT
    GGACAAAAGG GGTCCATTGG TGCACCTGGA ATTCCTGGAG TAGATGGTCA AAAGGGCGAG
    CAAGGAGAAG TTGGGACACC TGGCATTGAT GGAGTATCTG GAATGAAGGG TGATCCAGGT
    GAACAAGGAG ATAAGGGTGA TCCAGGGGAA GGCGGCCCTA AAGGAGATAT AGGAGCTAAG
    GGGGATCCAG GATCTTCTGC TGCGGGAATA AAGGGAGAGC CTGGAGAGCC TGGACGTTTT
    GGGCAGAAGG GTGAGCCAGG ATTTCTAGGG CCTCAAGGAG AACCTGGATT ACCAGGCTTA
    CCAGGAACTA AGGGGGAACG AGGAGAAATT GGTCCACCTG GAAGGGGAGA AAGAGGCAGT
    ACTGGACTAA CTGGACCAAA GGGGGAGCCT GGTGAATCCG GATCTAGAGG CCCAAAGGGG
    TCAAAGGGAG AATCTGGAAG CAAAGGAGAT TCTGGCCCCA TTGGTCCTAG GGGGCCTCCA
    GGTCAAAAAG GGGATCAAGG GGCTACTGAG ATCATAGATT ACAATGGGAA TATTCAGGAA
    GCCTTGCAGA GGATTACAGC CATTACTGTT ACGGGGCCGC CTGGACCTCC TGGACCACCT
    GGTTTGCCAG GTCCAAAGGG TGATGGAGGT CCTGTTGGTC CTCCAGGAGT TGATGGGGAG
    CAGGGTCCAA AAGGATCAAA GGGTGACCAT GGTGATTCCG GGACATCTGG AGAGAAGGGA
    GGAATTGGTC TCCCTGGTTT GCCGGGTGCA AATGGAATGA AAGGAGAGAA AGGAGATCAT
    GGTTCCCCTG GGCCCCATGG CGCTTCTATC ATTGGCCCCC CTGGACCTCC AGGGCCACAT
    GGCCCCCCTG GACCCATGGG ACCACATGGA CTTACAGGGC CAAAGGGTGA ACCAGGGGTA
    AATGGTCTTA AAGGATTGAG GGGTGAGCCA GGTCAAAAGG GTGAAAGAGG ACCACTGGGT
    CTTCCAGGAG CATCTGGCTT AGACGGAAAG CCAGGACCCA GGGGGAATGA TGGTCCCATT
    GGTGCTTCTG GTCCAGCAGG TCCAAAAGGA GACAGAGGAG AAAAAGGAGT TGTTGGTGAT
    CCCGGACCCA GAGGGCCTTA TGGTTTACCT GGCAAAGATG GAGAGCCTGG TCTAGACGGT
    TTTCCGGGTC CTCGTGGAGA AAAAGGTGAC CCTGGAGAAA GGGGAGAAAA GGGGGAAAAG
    GGAAAGAAAG GCAAAAAAGG GCCTAAAGGG GAGAAAGGAG AACAGGGTGC CCCTGGATTA
    GATGCACCTT GCCCATTGGG ACCAGATGGG CTTCCAATGC CTGGTTGTTG GCAAAAGGGA
    GTGACCCCAT GGCTTGTTGC CAAAAAGAGA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47634
    Clone ID Related Accession (Same CDS sequence) XM_031896009.1
    Accession Version XM_031896009.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2073bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(XXV) chain isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGTTGGGAA CAGGAGAGAT GAGAAAGAAT GGAGAGAAGT CCCAGGGATG TCACAGGGAC 
    AAGCTTGGAG GGGTGCTCAG TGGCTGGGTT AGGACTTTGC CCAGTGTCTT GGCATTTGTG
    TTCTCCCTGT TGTCCATGGC ATCATTTCTT TATCTGAATA TGAAGACCAA TGACCTGCAG
    GGAAGGGTTT GGGCACTGCA GCATGGCAAG TCTGGGGACA TTGCTTTCCA TATGCCAGGA
    TTATCTGTGG ATCAGCTTAA CTCCATCGTC CAGGAAAAAG TGGATCGCCT CTTGTCCCAG
    AGGTCCTATG AACACATGGC AAAGCTCCGA ATCGCAAGAG ATGCACCGGC AGACTGCAAC
    TGCCCAGCAG GCCCACCTGG ACAGCGAGGA AAAAGGGGGA GAAGAGGTGA ACCTGGACCA
    CCAGGTCCTC CTGGCCCAAA GGGAGACAAG GGTGATCAAG GTGATCAGGG ACCTCGGATG
    GTGTTTCCTA AGATCAATCA TGGCTTTCTC TCTGCTGATC AGCAGCTAAT AAAACGCCGC
    CTGATTAAGG GTGATCAAGG ACAAGCAGGC CCCCCGGGCC CCCCAGGTCC ACCGGGTCCA
    CCAGGTCCTA GGGGTCCTCC AGGAGACACT GGGAAAGATG GTCCAAGGGG ATTGTCTGGA
    GCTCCAGGGG AGACAGGGAA ACCAGGCGAA CAAGGCTTAA TGGGTCCAAT GGGGCCTCCT
    GGACAAAAGG GGTCCATTGG TGCACCTGGA ATTCCTGGAG TAGATGGTCA AAAGGGCGAG
    CAAGGAGAAG TTGGGACACC TGGCATTGAT GGAGTATCTG GAATGAAGGG TGATCCAGGT
    GAACAAGGAG ATAAGGGTGA TCCAGGGGAA GGCGGCCCTA AAGGAGATAT AGGAGCTAAG
    GGGGATCCAG GATCTTCTGC TGCGGGAATA AAGGGAGAGC CTGGAGAGCC TGGACGTTTT
    GGGCAGAAGG GTGAACCAGG GCTTCCTGGG CTTCCTGGAC TTCCTGGGAT TAAGGGTGAG
    CCAGGATTTC TAGGGCCTCA AGGAGAACCT GGATTACCAG GCTTACCAGG AACTAAGGGG
    GAACGAGGAG AAATTGGTCC ACCTGGAAGG GGAGAAAGAG GCAGTACTGG ACTAACTGGA
    CCAAAGGGAG AATCTGGAAG CAAAGGAGAT TCTGGCCCCA TTGGTCCTAG GGGGCCTCCA
    GGTCAAAAAG GGGATCAAGG GGCTACTGAG ATCATAGATT ACAATGGGAA TATTCAGGAA
    GCCTTGCAGA GGATTACAGC CATTACTGTT ACGGGGCCGC CTGGACCTCC TGGACCACCT
    GGTTTGCCAG GTCCAAAGGG TGATGGAGGT CCTGTTGGTC CTCCAGGAGT TGATGGGGAG
    CAGGGTCCAA AAGGATCAAA GGGTGACCAT GGTGATTCCG GGACATCTGG AGAGAAGGGA
    GGAATTGGTC TCCCTGGTTT GCCGGGTGCA AATGGAATGA AAGGAGAGAA AGGAGATCAT
    GGTTCCCCTG GGCCCCATGG CGCTTCTATC ATTGGCCCCC CTGGACCTCC AGGGCCACAT
    GGCCCCCCTG GACCCATGGG ACCACATGGA CTTACAGGGC CAAAGGGTGA ACCAGGGGTA
    AATGGTCTTA AAGGATTGAG GGGTGAGCCA GGTCAAAAGG GTGAAAGAGG ACCACTGGGT
    CTTCCAGGAG CATCTGGCTT AGACGGAAAG CCAGGACCCA GGGGGAATGA TGGTCCCATT
    GGTGCTTCTG GTCCAGCAGG TCCAAAAGGA GACAGAGGAG AAAAAGGAGT TGTTGGTGAT
    CCCGGACCCA GAGGGCCTTA TGGTTTACCT GGCAAAGATG GAGAGCCTGG TCTAGACGGT
    TTTCCGGGTC CTCGTGGAGA AAAAGGTGAC CCTGGAGAAA GGGGAGAAAA GGGGGAAAAG
    GGAAAGAAAG GCAAAAAAGG GCCTAAAGGG GAGAAAGGAG AACAGGGTGC CCCTGGATTA
    GATGCACCTT GCCCATTGGG ACCAGATGGG CTTCCAATGC CTGGTTGTTG GCAAAAGGGA
    GTGACCCCAT GGCTTGTTGC CAAAAAGAGA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751869.1
    CDS480..2552
    Translation

    Target ORF information:

    RefSeq Version XM_031896009.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type XXV, alpha 1, gene 2 (col25a1.2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896009.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGTTGGGAA CAGGAGAGAT GAGAAAGAAT GGAGAGAAGT CCCAGGGATG TCACAGGGAC 
    AAGCTTGGAG GGGTGCTCAG TGGCTGGGTT AGGACTTTGC CCAGTGTCTT GGCATTTGTG
    TTCTCCCTGT TGTCCATGGC ATCATTTCTT TATCTGAATA TGAAGACCAA TGACCTGCAG
    GGAAGGGTTT GGGCACTGCA GCATGGCAAG TCTGGGGACA TTGCTTTCCA TATGCCAGGA
    TTATCTGTGG ATCAGCTTAA CTCCATCGTC CAGGAAAAAG TGGATCGCCT CTTGTCCCAG
    AGGTCCTATG AACACATGGC AAAGCTCCGA ATCGCAAGAG ATGCACCGGC AGACTGCAAC
    TGCCCAGCAG GCCCACCTGG ACAGCGAGGA AAAAGGGGGA GAAGAGGTGA ACCTGGACCA
    CCAGGTCCTC CTGGCCCAAA GGGAGACAAG GGTGATCAAG GTGATCAGGG ACCTCGGATG
    GTGTTTCCTA AGATCAATCA TGGCTTTCTC TCTGCTGATC AGCAGCTAAT AAAACGCCGC
    CTGATTAAGG GTGATCAAGG ACAAGCAGGC CCCCCGGGCC CCCCAGGTCC ACCGGGTCCA
    CCAGGTCCTA GGGGTCCTCC AGGAGACACT GGGAAAGATG GTCCAAGGGG ATTGTCTGGA
    GCTCCAGGGG AGACAGGGAA ACCAGGCGAA CAAGGCTTAA TGGGTCCAAT GGGGCCTCCT
    GGACAAAAGG GGTCCATTGG TGCACCTGGA ATTCCTGGAG TAGATGGTCA AAAGGGCGAG
    CAAGGAGAAG TTGGGACACC TGGCATTGAT GGAGTATCTG GAATGAAGGG TGATCCAGGT
    GAACAAGGAG ATAAGGGTGA TCCAGGGGAA GGCGGCCCTA AAGGAGATAT AGGAGCTAAG
    GGGGATCCAG GATCTTCTGC TGCGGGAATA AAGGGAGAGC CTGGAGAGCC TGGACGTTTT
    GGGCAGAAGG GTGAACCAGG GCTTCCTGGG CTTCCTGGAC TTCCTGGGAT TAAGGGTGAG
    CCAGGATTTC TAGGGCCTCA AGGAGAACCT GGATTACCAG GCTTACCAGG AACTAAGGGG
    GAACGAGGAG AAATTGGTCC ACCTGGAAGG GGAGAAAGAG GCAGTACTGG ACTAACTGGA
    CCAAAGGGAG AATCTGGAAG CAAAGGAGAT TCTGGCCCCA TTGGTCCTAG GGGGCCTCCA
    GGTCAAAAAG GGGATCAAGG GGCTACTGAG ATCATAGATT ACAATGGGAA TATTCAGGAA
    GCCTTGCAGA GGATTACAGC CATTACTGTT ACGGGGCCGC CTGGACCTCC TGGACCACCT
    GGTTTGCCAG GTCCAAAGGG TGATGGAGGT CCTGTTGGTC CTCCAGGAGT TGATGGGGAG
    CAGGGTCCAA AAGGATCAAA GGGTGACCAT GGTGATTCCG GGACATCTGG AGAGAAGGGA
    GGAATTGGTC TCCCTGGTTT GCCGGGTGCA AATGGAATGA AAGGAGAGAA AGGAGATCAT
    GGTTCCCCTG GGCCCCATGG CGCTTCTATC ATTGGCCCCC CTGGACCTCC AGGGCCACAT
    GGCCCCCCTG GACCCATGGG ACCACATGGA CTTACAGGGC CAAAGGGTGA ACCAGGGGTA
    AATGGTCTTA AAGGATTGAG GGGTGAGCCA GGTCAAAAGG GTGAAAGAGG ACCACTGGGT
    CTTCCAGGAG CATCTGGCTT AGACGGAAAG CCAGGACCCA GGGGGAATGA TGGTCCCATT
    GGTGCTTCTG GTCCAGCAGG TCCAAAAGGA GACAGAGGAG AAAAAGGAGT TGTTGGTGAT
    CCCGGACCCA GAGGGCCTTA TGGTTTACCT GGCAAAGATG GAGAGCCTGG TCTAGACGGT
    TTTCCGGGTC CTCGTGGAGA AAAAGGTGAC CCTGGAGAAA GGGGAGAAAA GGGGGAAAAG
    GGAAAGAAAG GCAAAAAAGG GCCTAAAGGG GAGAAAGGAG AACAGGGTGC CCCTGGATTA
    GATGCACCTT GCCCATTGGG ACCAGATGGG CTTCCAATGC CTGGTTGTTG GCAAAAGGGA
    GTGACCCCAT GGCTTGTTGC CAAAAAGAGA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47635
    Clone ID Related Accession (Same CDS sequence) XM_031896010.1
    Accession Version XM_031896010.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2055bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(XXV) chain isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGTTGGGAA CAGGAGAGAT GAGAAAGAAT GGAGAGAAGT CCCAGGGATG TCACAGGGAC 
    AAGCTTGGAG GGGTGCTCAG TGGCTGGGTT AGGACTTTGC CCAGTGTCTT GGCATTTGTG
    TTCTCCCTGT TGTCCATGGC ATCATTTCTT TATCTGAATA TGAAGACCAA TGACCTGCAG
    GGAAGGGTTT GGGCACTGCA GCATGGCAAG TCTGGGGACA TTGCTTTCCA TATGCCAGGA
    TTATCTGTGG ATCAGCTTAA CTCCATCGTC CAGGAAAAAG TGGATCGCCT CTTGTCCCAG
    AGGTCCTATG AACACATGGC AAAGCTCCGA ATCGCAAGAG ATGCACCGGC AGACTGCAAC
    TGCCCAGCAG GCCCACCTGG ACAGCGAGGA AAAAGGGGGA GAAGAGGTGA ACCTGGACCA
    CCAGGTCCTC CTGGCCCAAA GGGAGACAAG GGTGATCAAG GTGATCAGGG ACCTCGGATG
    GTGTTTCCTA AGATCAATCA TGGCTTTCTC TCTGCTGATC AGCAGCTAAT AAAACGCCGC
    CTGATTAAGG GTGATCAAGG ACAAGCAGGC CCCCCGGGCC CCCCAGGTCC ACCGGGTCCA
    CCAGGTCCTA GGGGTCCTCC AGGAGACACT GGGAAAGATG GTCCAAGGGG ATTGTCTGGA
    GCTCCAGGGG AGACAGGGAA ACCAGGCGAA CAAGGCTTAA TGGGTCCAAT GGGGCCTCCT
    GGACAAAAGG GGTCCATTGG TGCACCTGGA ATTCCTGGAG TAGATGGTCA AAAGGGCGAG
    CAAGGAGAAG TTGGGACACC TGGCATTGAT GGAGTATCTG GAATGAAGGG TGATCCAGGT
    GAACAAGGAG ATAAGGGTGA TCCAGGGGAA GGCGGCCCTA AAGGAGATAT AGGAGCTAAG
    GGGGATCCAG GATCTTCTGC TGCGGGAATA AAGGGAGAGC CTGGAGAGCC TGGACGTTTT
    GGGCAGAAGG GTGAACCAGG GCTTCCTGGG CTTCCTGGAC TTCCTGGGAT TAAGGGTGAG
    CCAGGATTTC TAGGGCCTCA AGGAGAACCT GGATTACCAG GCTTACCAGG AACTAAGGGG
    GAACGAGGAG AAATTGGTCC ACCTGGAAGG GGAGAAAGAG GCAGTACTGG ACTAACTGGA
    CCAAAGGGGG AGCCTGGTGA ATCCGGATCT AGAGGCCCAA AGGGGTCAAA GGGAGAATCT
    GGAAGCAAAG GAGATTCTGG CCCCATTGGT CCTAGGGGGC CTCCAGGTCA AAAAGGGGAT
    CAAGGGGCTA CTGAGATCAT AGATTACAAT GGGAATATTC AGGAAGCCTT GCAGAGGATT
    ACAGCCATTA CTGTTACGGG GCCGCCTGGA CCTCCTGGAC CACCTGGTTT GCCAGGTCCA
    AAGGGTGATG GAGGTCCTGT TGGTCCTCCA GGAGTTGATG GGGAGCAGGG TCCAAAAGGA
    TCAAAGGGTG ACCATGGTGA TTCCGGGACA TCTGGAGAGA AGGGAGGAAT TGGTCTCCCT
    GGTTTGCCGG GTGCAAATGG AATGAAAGGA GAGAAAGGAG ATCATGGTTC CCCTGGGCCC
    CATGGCGCTT CTATCATTGG CCCCCCTGGA CCTCCAGGGC CACATGGCCC CCCTGGACCC
    ATGGGACCAC ATGGACTTAC AGGGCCAAAG GGTGAACCAG GGGTAAATGG TCTTAAAGGA
    TTGAGGGGTG AGCCAGGTCA AAAGGGTGAA AGAGGACCAC TGGGTCTTCC AGGAGCATCT
    GGCTTAGACG GAAAGCCAGG ACCCAGGGGG AATGATGGTC CCATTGGTGC TTCTGGTCCA
    GCAGGTCCAA AAGGAGACAG AGGAGAAAAA GGAGTTGTTG GTGATCCCGG ACCCAGAGGG
    CCTTATGGTT TACCTGGCAA AGATGGAGAG CCTGGTCTAG ACGGTTTCCG TGGATTAAAG
    GGGGAAAAAG GGGAACCAGG TCAGCCTGGC CTGGATGGGC TGGATGCCCC TTGCCAATTG
    GGACCAGATG GGCTTCCAAT GCCTGGTTGT TGGCAAAAGG GAGTGACCCC ATGGCTTGTT
    GCCAAAAAGA GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751870.1
    CDS480..2534
    Translation

    Target ORF information:

    RefSeq Version XM_031896010.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type XXV, alpha 1, gene 2 (col25a1.2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896010.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGTTGGGAA CAGGAGAGAT GAGAAAGAAT GGAGAGAAGT CCCAGGGATG TCACAGGGAC 
    AAGCTTGGAG GGGTGCTCAG TGGCTGGGTT AGGACTTTGC CCAGTGTCTT GGCATTTGTG
    TTCTCCCTGT TGTCCATGGC ATCATTTCTT TATCTGAATA TGAAGACCAA TGACCTGCAG
    GGAAGGGTTT GGGCACTGCA GCATGGCAAG TCTGGGGACA TTGCTTTCCA TATGCCAGGA
    TTATCTGTGG ATCAGCTTAA CTCCATCGTC CAGGAAAAAG TGGATCGCCT CTTGTCCCAG
    AGGTCCTATG AACACATGGC AAAGCTCCGA ATCGCAAGAG ATGCACCGGC AGACTGCAAC
    TGCCCAGCAG GCCCACCTGG ACAGCGAGGA AAAAGGGGGA GAAGAGGTGA ACCTGGACCA
    CCAGGTCCTC CTGGCCCAAA GGGAGACAAG GGTGATCAAG GTGATCAGGG ACCTCGGATG
    GTGTTTCCTA AGATCAATCA TGGCTTTCTC TCTGCTGATC AGCAGCTAAT AAAACGCCGC
    CTGATTAAGG GTGATCAAGG ACAAGCAGGC CCCCCGGGCC CCCCAGGTCC ACCGGGTCCA
    CCAGGTCCTA GGGGTCCTCC AGGAGACACT GGGAAAGATG GTCCAAGGGG ATTGTCTGGA
    GCTCCAGGGG AGACAGGGAA ACCAGGCGAA CAAGGCTTAA TGGGTCCAAT GGGGCCTCCT
    GGACAAAAGG GGTCCATTGG TGCACCTGGA ATTCCTGGAG TAGATGGTCA AAAGGGCGAG
    CAAGGAGAAG TTGGGACACC TGGCATTGAT GGAGTATCTG GAATGAAGGG TGATCCAGGT
    GAACAAGGAG ATAAGGGTGA TCCAGGGGAA GGCGGCCCTA AAGGAGATAT AGGAGCTAAG
    GGGGATCCAG GATCTTCTGC TGCGGGAATA AAGGGAGAGC CTGGAGAGCC TGGACGTTTT
    GGGCAGAAGG GTGAACCAGG GCTTCCTGGG CTTCCTGGAC TTCCTGGGAT TAAGGGTGAG
    CCAGGATTTC TAGGGCCTCA AGGAGAACCT GGATTACCAG GCTTACCAGG AACTAAGGGG
    GAACGAGGAG AAATTGGTCC ACCTGGAAGG GGAGAAAGAG GCAGTACTGG ACTAACTGGA
    CCAAAGGGGG AGCCTGGTGA ATCCGGATCT AGAGGCCCAA AGGGGTCAAA GGGAGAATCT
    GGAAGCAAAG GAGATTCTGG CCCCATTGGT CCTAGGGGGC CTCCAGGTCA AAAAGGGGAT
    CAAGGGGCTA CTGAGATCAT AGATTACAAT GGGAATATTC AGGAAGCCTT GCAGAGGATT
    ACAGCCATTA CTGTTACGGG GCCGCCTGGA CCTCCTGGAC CACCTGGTTT GCCAGGTCCA
    AAGGGTGATG GAGGTCCTGT TGGTCCTCCA GGAGTTGATG GGGAGCAGGG TCCAAAAGGA
    TCAAAGGGTG ACCATGGTGA TTCCGGGACA TCTGGAGAGA AGGGAGGAAT TGGTCTCCCT
    GGTTTGCCGG GTGCAAATGG AATGAAAGGA GAGAAAGGAG ATCATGGTTC CCCTGGGCCC
    CATGGCGCTT CTATCATTGG CCCCCCTGGA CCTCCAGGGC CACATGGCCC CCCTGGACCC
    ATGGGACCAC ATGGACTTAC AGGGCCAAAG GGTGAACCAG GGGTAAATGG TCTTAAAGGA
    TTGAGGGGTG AGCCAGGTCA AAAGGGTGAA AGAGGACCAC TGGGTCTTCC AGGAGCATCT
    GGCTTAGACG GAAAGCCAGG ACCCAGGGGG AATGATGGTC CCATTGGTGC TTCTGGTCCA
    GCAGGTCCAA AAGGAGACAG AGGAGAAAAA GGAGTTGTTG GTGATCCCGG ACCCAGAGGG
    CCTTATGGTT TACCTGGCAA AGATGGAGAG CCTGGTCTAG ACGGTTTCCG TGGATTAAAG
    GGGGAAAAAG GGGAACCAGG TCAGCCTGGC CTGGATGGGC TGGATGCCCC TTGCCAATTG
    GGACCAGATG GGCTTCCAAT GCCTGGTTGT TGGCAAAAGG GAGTGACCCC ATGGCTTGTT
    GCCAAAAAGA GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47636
    Clone ID Related Accession (Same CDS sequence) XM_031896011.1
    Accession Version XM_031896011.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2001bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(XXV) chain isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGTTGGGAA CAGGAGAGAT GAGAAAGAAT GGAGAGAAGT CCCAGGGATG TCACAGGGAC 
    AAGCTTGGAG GGGTGCTCAG TGGCTGGGTT AGGACTTTGC CCAGTGTCTT GGCATTTGTG
    TTCTCCCTGT TGTCCATGGC ATCATTTCTT TATCTGAATA TGAAGACCAA TGACCTGCAG
    GGAAGGGTTT GGGCACTGCA GCATGGCAAG TCTGGGGACA TTGCTTTCCA TATGCCAGGA
    TTATCTGTGG ATCAGCTTAA CTCCATCGTC CAGGAAAAAG TGGATCGCCT CTTGTCCCAG
    AGGTCCTATG AACACATGGC AAAGCTCCGA ATCGCAAGAG ATGCACCGGC AGACTGCAAC
    TGCCCAGCAG GCCCACCTGG ACAGCGAGGA AAAAGGGGGA GAAGAGGTGA ACCTGGACCA
    CCAGGTCCTC CTGGCCCAAA GGGAGACAAG GGTGATCAAG GTGATCAGGG ACCTCGGATG
    GTGTTTCCTA AGATCAATCA TGGCTTTCTC TCTGCTGATC AGCAGCTAAT AAAACGCCGC
    CTGATTAAGG GTGATCAAGG ACAAGCAGGC CCCCCGGGCC CCCCAGGTCC ACCGGGTCCA
    CCAGGTCCTA GGGGTCCTCC AGGAGACACT GGGAAAGATG GTCCAAGGGG ATTGTCTGGA
    GCTCCAGGGG AGACAGGGAA ACCAGGCGAA CAAGGCTTAA TGGGTCCAAT GGGGCCTCCT
    GGACAAAAGG GGTCCATTGG TGCACCTGGA ATTCCTGGAG TAGATGGTCA AAAGGGCGAG
    CAAGGAGAAG TTGGGACACC TGGCATTGAT GGAGTATCTG GAATGAAGGG TGATCCAGGT
    GAACAAGGAG ATAAGGGTGA TCCAGGGGAA GGCGGCCCTA AAGGAGATAT AGGAGCTAAG
    GGGGATCCAG GATCTTCTGC TGCGGGAATA AAGGGAGAGC CTGGAGAGCC TGGACGTTTT
    GGGCAGAAGG GTGAACCAGG GCTTCCTGGG CTTCCTGGAC TTCCTGGGAT TAAGGGTGAG
    CCAGGATTTC TAGGGCCTCA AGGAGAACCT GGATTACCAG GCTTACCAGG AACTAAGGGG
    GAACGAGGAG AAATTGGTCC ACCTGGAAGG GGAGAAAGAG GCAGTACTGG ACTAACTGGA
    CCAAAGGGGG AGCCTGGTGA ATCCGGATCT AGAGGCCCAA AGGGGTCAAA GGGAGAATCT
    GGAAGCAAAG GAGATTCTGG CCCCATTGGT CCTAGGGGGC CTCCAGGTCA AAAAGGGGAT
    CAAGGGGCTA CTGAGATCAT AGATTACAAT GGGAATATTC AGGAAGCCTT GCAGAGGATT
    ACAGCCATTA CTGTTACGGG GCCGCCTGGA CCTCCTGGAC CACCTGGTTT GCCAGGTCCA
    AAGGGTGATG GAGGTCCTGT TGGTCCTCCA GGAGTTGATG GGGAGCAGGG TCCAAAAGGA
    TCAAAGGGTG ACCATGGTGA TTCCGGGACA TCTGGAGAGA AGGGAGGAAT TGGTCTCCCT
    GGTTTGCCGG GTGCAAATGG AATGAAAGGA GAGAAAGGAG ATCATGGTTC CCCTGGGCCC
    CATGGCGCTT CTATCATTGG CCCCCCTGGA CCTCCAGGGC CACATGGCCC CCCTGGACCC
    ATGGGACCAC ATGGACTTAC AGGGCCAAAG GGGAATGATG GTCCCATTGG TGCTTCTGGT
    CCAGCAGGTC CAAAAGGAGA CAGAGGAGAA AAAGGAGTTG TTGGTGATCC CGGACCCAGA
    GGGCCTTATG GTTTACCTGG CAAAGATGGA GAGCCTGGTC TAGACGGTTT TCCGGGTCCT
    CGTGGAGAAA AAGGTGACCC TGGAGAAAGG GGAGAAAAGG GGGAAAAGGG AAAGAAAGGC
    AAAAAAGGGC CTAAAGGGGA GAAAGGAGAA CAGGGTGCCC CTGGATTAGA TGCACCTTGC
    CCATTGGGAC CAGATGGGCT TCCAATGCCT GGTTGTTGGC AAAAGGGAGT GACCCCATGG
    CTTGTTGCCA AAAAGAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751871.1
    CDS480..2480
    Translation

    Target ORF information:

    RefSeq Version XM_031896011.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type XXV, alpha 1, gene 2 (col25a1.2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896011.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGTTGGGAA CAGGAGAGAT GAGAAAGAAT GGAGAGAAGT CCCAGGGATG TCACAGGGAC 
    AAGCTTGGAG GGGTGCTCAG TGGCTGGGTT AGGACTTTGC CCAGTGTCTT GGCATTTGTG
    TTCTCCCTGT TGTCCATGGC ATCATTTCTT TATCTGAATA TGAAGACCAA TGACCTGCAG
    GGAAGGGTTT GGGCACTGCA GCATGGCAAG TCTGGGGACA TTGCTTTCCA TATGCCAGGA
    TTATCTGTGG ATCAGCTTAA CTCCATCGTC CAGGAAAAAG TGGATCGCCT CTTGTCCCAG
    AGGTCCTATG AACACATGGC AAAGCTCCGA ATCGCAAGAG ATGCACCGGC AGACTGCAAC
    TGCCCAGCAG GCCCACCTGG ACAGCGAGGA AAAAGGGGGA GAAGAGGTGA ACCTGGACCA
    CCAGGTCCTC CTGGCCCAAA GGGAGACAAG GGTGATCAAG GTGATCAGGG ACCTCGGATG
    GTGTTTCCTA AGATCAATCA TGGCTTTCTC TCTGCTGATC AGCAGCTAAT AAAACGCCGC
    CTGATTAAGG GTGATCAAGG ACAAGCAGGC CCCCCGGGCC CCCCAGGTCC ACCGGGTCCA
    CCAGGTCCTA GGGGTCCTCC AGGAGACACT GGGAAAGATG GTCCAAGGGG ATTGTCTGGA
    GCTCCAGGGG AGACAGGGAA ACCAGGCGAA CAAGGCTTAA TGGGTCCAAT GGGGCCTCCT
    GGACAAAAGG GGTCCATTGG TGCACCTGGA ATTCCTGGAG TAGATGGTCA AAAGGGCGAG
    CAAGGAGAAG TTGGGACACC TGGCATTGAT GGAGTATCTG GAATGAAGGG TGATCCAGGT
    GAACAAGGAG ATAAGGGTGA TCCAGGGGAA GGCGGCCCTA AAGGAGATAT AGGAGCTAAG
    GGGGATCCAG GATCTTCTGC TGCGGGAATA AAGGGAGAGC CTGGAGAGCC TGGACGTTTT
    GGGCAGAAGG GTGAACCAGG GCTTCCTGGG CTTCCTGGAC TTCCTGGGAT TAAGGGTGAG
    CCAGGATTTC TAGGGCCTCA AGGAGAACCT GGATTACCAG GCTTACCAGG AACTAAGGGG
    GAACGAGGAG AAATTGGTCC ACCTGGAAGG GGAGAAAGAG GCAGTACTGG ACTAACTGGA
    CCAAAGGGGG AGCCTGGTGA ATCCGGATCT AGAGGCCCAA AGGGGTCAAA GGGAGAATCT
    GGAAGCAAAG GAGATTCTGG CCCCATTGGT CCTAGGGGGC CTCCAGGTCA AAAAGGGGAT
    CAAGGGGCTA CTGAGATCAT AGATTACAAT GGGAATATTC AGGAAGCCTT GCAGAGGATT
    ACAGCCATTA CTGTTACGGG GCCGCCTGGA CCTCCTGGAC CACCTGGTTT GCCAGGTCCA
    AAGGGTGATG GAGGTCCTGT TGGTCCTCCA GGAGTTGATG GGGAGCAGGG TCCAAAAGGA
    TCAAAGGGTG ACCATGGTGA TTCCGGGACA TCTGGAGAGA AGGGAGGAAT TGGTCTCCCT
    GGTTTGCCGG GTGCAAATGG AATGAAAGGA GAGAAAGGAG ATCATGGTTC CCCTGGGCCC
    CATGGCGCTT CTATCATTGG CCCCCCTGGA CCTCCAGGGC CACATGGCCC CCCTGGACCC
    ATGGGACCAC ATGGACTTAC AGGGCCAAAG GGGAATGATG GTCCCATTGG TGCTTCTGGT
    CCAGCAGGTC CAAAAGGAGA CAGAGGAGAA AAAGGAGTTG TTGGTGATCC CGGACCCAGA
    GGGCCTTATG GTTTACCTGG CAAAGATGGA GAGCCTGGTC TAGACGGTTT TCCGGGTCCT
    CGTGGAGAAA AAGGTGACCC TGGAGAAAGG GGAGAAAAGG GGGAAAAGGG AAAGAAAGGC
    AAAAAAGGGC CTAAAGGGGA GAAAGGAGAA CAGGGTGCCC CTGGATTAGA TGCACCTTGC
    CCATTGGGAC CAGATGGGCT TCCAATGCCT GGTTGTTGGC AAAAGGGAGT GACCCCATGG
    CTTGTTGCCA AAAAGAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47637
    Clone ID Related Accession (Same CDS sequence) XM_031896014.1
    Accession Version XM_031896014.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1986bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(XXV) chain isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGTTGGGAA CAGGAGAGAT GAGAAAGAAT GGAGAGAAGT CCCAGGGATG TCACAGGGAC 
    AAGCTTGGAG GGGTGCTCAG TGGCTGGGTT AGGACTTTGC CCAGTGTCTT GGCATTTGTG
    TTCTCCCTGT TGTCCATGGC ATCATTTCTT TATCTGAATA TGAAGACCAA TGACCTGCAG
    GGAAGGGTTT GGGCACTGCA GCATGGCAAG TCTGGGGACA TTGCTTTCCA TATGCCAGGA
    TTATCTGTGG ATCAGCTTAA CTCCATCGTC CAGGAAAAAG TGGATCGCCT CTTGTCCCAG
    AGGTCCTATG AACACATGGC AAAGCTCCGA ATCGCAAGAG ATGCACCGGC AGACTGCAAC
    TGCCCAGCAG GCCCACCTGG ACAGCGAGGA AAAAGGGGGA GAAGAGGTGA ACCTGGACCA
    CCAGGTCCTC CTGGCCCAAA GGGAGACAAG GGTGATCAAG GTGATCAGGG ACCTCGGATG
    GTGTTTCCTA AGATCAATCA TGGCTTTCTC TCTGCTGATC AGCAGCTAAT AAAACGCCGC
    CTGATTAAGG GTGATCAAGG ACAAGCAGGC CCCCCGGGCC CCCCAGGTCC ACCGGGTCCA
    CCAGGTCCTA GGGGTCCTCC AGGAGACACT GGGAAAGATG GTCCAAGGGG ATTGTCTGGA
    GCTCCAGGGG AGACAGGGAA ACCAGGCGAA CAAGGCTTAA TGGGTCCAAT GGGGCCTCCT
    GGACAAAAGG GGTCCATTGG TGCACCTGGA ATTCCTGGAG TAGATGGTCA AAAGGGCGAG
    CAAGGAGAAG TTGGGACACC TGGCATTGAT GGAGTATCTG GAATGAAGGG TGATCCAGGT
    GAACAAGGAG ATAAGGGTGA TCCAGGGGAA GGCGGCCCTA AAGGAGATAT AGGAGCTAAG
    GGGGATCCAG GATCTTCTGC TGCGGGAATA AAGGGAGAGC CTGGAGAGCC TGGACGTTTT
    GGGCAGAAGG GTGAACCAGG GCTTCCTGGG CTTCCTGGAC TTCCTGGGAT TAAGGGTGAG
    CCAGGATTTC TAGGGCCTCA AGGAGAACCT GGATTACCAG GCTTACCAGG AACTAAGGGG
    GAACGAGGAG AAATTGGTCC ACCTGGAAGG GGAGAAAGAG GCAGTACTGG ACTAACTGGA
    CCAAAGGGGG AGCCTGGTGA ATCCGGATCT AGAGGCCCAA AGGGGTCAAA GGGAGAATCT
    GGAAGCAAAG GAGATTCTGG CCCCATTGGT CCTAGGGGGC CTCCAGGTCA AAAAGGGGAT
    CAAGGGGCTA CTGAGATCAT AGATTACAAT GGGAATATTC AGGAAGCCTT GCAGAGGATT
    ACAGCCATTA CTGTTACGGG GCCGCCTGGA CCTCCTGGAC CACCTGGTTT GCCAGGTCCA
    AAGGGTGATG GAGGTCCTGT TGGTCCTCCA GGAGTTGATG GGGAGCAGGG TCCAAAAGGA
    TCAAAGGGTG ACCATGGTGA TTCCGGGACA TCTGGAGAGA AGGGAGGAAT TGGTCTCCCT
    GGTTTGCCGG GTGCAAATGG AATGAAAGGA GAGAAAGGAG ATCATGGTTC CCCTGGGCCC
    CATGGCGCTT CTATCATTGG CCCCCCTGGA CCTCCAGGGC CACATGGCCC CCCTGGACCC
    ATGGGACCAC ATGGACTTAC AGGGCCAAAG GGTGAACCAG GGGTAAATGG TCTTAAAGGA
    TTGAGGGGTG AGCCAGGTCA AAAGGGTGAA AGAGGACCAC TGGGTCTTCC AGGGAATGAT
    GGTCCCATTG GTGCTTCTGG TCCAGCAGGT CCAAAAGGAG ACAGAGGAGA AAAAGGAGTT
    GTTGGTGATC CCGGACCCAG AGGGCCTTAT GGTTTACCTG GCAAAGATGG AGAGCCTGGT
    CTAGACGGTT TCCGTGGATT AAAGGGGGAA AAAGGGGAAC CAGGTCAGCC TGGCCTGGAT
    GGGCTGGATG CCCCTTGCCA ATTGGGACCA GATGGGCTTC CAATGCCTGG TTGTTGGCAA
    AAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751874.1
    CDS480..2465
    Translation

    Target ORF information:

    RefSeq Version XM_031896014.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type XXV, alpha 1, gene 2 (col25a1.2), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896014.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGTTGGGAA CAGGAGAGAT GAGAAAGAAT GGAGAGAAGT CCCAGGGATG TCACAGGGAC 
    AAGCTTGGAG GGGTGCTCAG TGGCTGGGTT AGGACTTTGC CCAGTGTCTT GGCATTTGTG
    TTCTCCCTGT TGTCCATGGC ATCATTTCTT TATCTGAATA TGAAGACCAA TGACCTGCAG
    GGAAGGGTTT GGGCACTGCA GCATGGCAAG TCTGGGGACA TTGCTTTCCA TATGCCAGGA
    TTATCTGTGG ATCAGCTTAA CTCCATCGTC CAGGAAAAAG TGGATCGCCT CTTGTCCCAG
    AGGTCCTATG AACACATGGC AAAGCTCCGA ATCGCAAGAG ATGCACCGGC AGACTGCAAC
    TGCCCAGCAG GCCCACCTGG ACAGCGAGGA AAAAGGGGGA GAAGAGGTGA ACCTGGACCA
    CCAGGTCCTC CTGGCCCAAA GGGAGACAAG GGTGATCAAG GTGATCAGGG ACCTCGGATG
    GTGTTTCCTA AGATCAATCA TGGCTTTCTC TCTGCTGATC AGCAGCTAAT AAAACGCCGC
    CTGATTAAGG GTGATCAAGG ACAAGCAGGC CCCCCGGGCC CCCCAGGTCC ACCGGGTCCA
    CCAGGTCCTA GGGGTCCTCC AGGAGACACT GGGAAAGATG GTCCAAGGGG ATTGTCTGGA
    GCTCCAGGGG AGACAGGGAA ACCAGGCGAA CAAGGCTTAA TGGGTCCAAT GGGGCCTCCT
    GGACAAAAGG GGTCCATTGG TGCACCTGGA ATTCCTGGAG TAGATGGTCA AAAGGGCGAG
    CAAGGAGAAG TTGGGACACC TGGCATTGAT GGAGTATCTG GAATGAAGGG TGATCCAGGT
    GAACAAGGAG ATAAGGGTGA TCCAGGGGAA GGCGGCCCTA AAGGAGATAT AGGAGCTAAG
    GGGGATCCAG GATCTTCTGC TGCGGGAATA AAGGGAGAGC CTGGAGAGCC TGGACGTTTT
    GGGCAGAAGG GTGAACCAGG GCTTCCTGGG CTTCCTGGAC TTCCTGGGAT TAAGGGTGAG
    CCAGGATTTC TAGGGCCTCA AGGAGAACCT GGATTACCAG GCTTACCAGG AACTAAGGGG
    GAACGAGGAG AAATTGGTCC ACCTGGAAGG GGAGAAAGAG GCAGTACTGG ACTAACTGGA
    CCAAAGGGGG AGCCTGGTGA ATCCGGATCT AGAGGCCCAA AGGGGTCAAA GGGAGAATCT
    GGAAGCAAAG GAGATTCTGG CCCCATTGGT CCTAGGGGGC CTCCAGGTCA AAAAGGGGAT
    CAAGGGGCTA CTGAGATCAT AGATTACAAT GGGAATATTC AGGAAGCCTT GCAGAGGATT
    ACAGCCATTA CTGTTACGGG GCCGCCTGGA CCTCCTGGAC CACCTGGTTT GCCAGGTCCA
    AAGGGTGATG GAGGTCCTGT TGGTCCTCCA GGAGTTGATG GGGAGCAGGG TCCAAAAGGA
    TCAAAGGGTG ACCATGGTGA TTCCGGGACA TCTGGAGAGA AGGGAGGAAT TGGTCTCCCT
    GGTTTGCCGG GTGCAAATGG AATGAAAGGA GAGAAAGGAG ATCATGGTTC CCCTGGGCCC
    CATGGCGCTT CTATCATTGG CCCCCCTGGA CCTCCAGGGC CACATGGCCC CCCTGGACCC
    ATGGGACCAC ATGGACTTAC AGGGCCAAAG GGTGAACCAG GGGTAAATGG TCTTAAAGGA
    TTGAGGGGTG AGCCAGGTCA AAAGGGTGAA AGAGGACCAC TGGGTCTTCC AGGGAATGAT
    GGTCCCATTG GTGCTTCTGG TCCAGCAGGT CCAAAAGGAG ACAGAGGAGA AAAAGGAGTT
    GTTGGTGATC CCGGACCCAG AGGGCCTTAT GGTTTACCTG GCAAAGATGG AGAGCCTGGT
    CTAGACGGTT TCCGTGGATT AAAGGGGGAA AAAGGGGAAC CAGGTCAGCC TGGCCTGGAT
    GGGCTGGATG CCCCTTGCCA ATTGGGACCA GATGGGCTTC CAATGCCTGG TTGTTGGCAA
    AAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.