washc2a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following washc2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the washc2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa45576 XM_018095644.1
Latest version!
Xenopus tropicalis family with sequence similarity 21 member A (fam21a), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OXa62873 XM_031906385.1
Latest version!
Xenopus tropicalis WASH complex subunit 2A (washc2a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa62874 XM_031906386.1
Latest version!
Xenopus tropicalis WASH complex subunit 2A (washc2a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa62875 XM_031906387.1
Latest version!
Xenopus tropicalis WASH complex subunit 2A (washc2a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa45576
    Clone ID Related Accession (Same CDS sequence) XM_018095644.1
    Accession Version XM_018095644.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3786bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product WASH complex subunit FAM21C
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAACGGGC CGGCGAATGG TTCGGCAGAG ACGCCAGTGT GGGAGAGACC CTGGGGCTTG 
    GAGGAGATTA GGAGGAGCAG CCAGAACTGG TCCCTGGGTG CTGATGCGGG GCTCTTGAAT
    TTCCTCCGGG AGTTCTCTCA GCAAACCATA TCAAGGACAC ATGAAATAGA GAAACAACTG
    GATGGGCTCA TTCGTGAAAC AAAGGCCACA GACTGTCGTC TACACAATGT CTTTAATGAT
    TTTCTTATGT TATCTAACAC TCAGTTCATA GAAAATAGAG TGTATGATGA AGAAGTTGAA
    GATCATGTTG TTAAGGCAGA TGCTGGGAAC AAACCTGAAC AGGAGAAAAC TCGGGAGCAA
    AAGGAAGCAG AGCTGATTCC AAAAATACAG GAAGCTGTAA ACTATGGACT GCAAGTACTG
    GAAAATGCTT TTGAGCAACT GGATATTAAA GCAGGGAATT CTGACTCGGA GGAAGAAGAA
    GTTAATGAGC GTGTGGAGCC TATATTAGAG CCAAAGGATC TTTATATAGA CAGACCTCTG
    CCGCTTTTAA TTGGCTCCCA ACAGTTCATG CAGCAAGAAG ATGTTGGCCT TGGAGATCTC
    TCAAGTGAAG AGGGATCGGT GGACAATGAT CGGGGGAGCA TTATTGACAG TGAAGATGAA
    AAAGATGATG AGTTCATCCA GTGCAATTAT TTAAATCCGC TGCATTTGTT TTCAGCTGTT
    GCATCTCTGG AGGTGAAAAA AAAGAAAGAA AAAGATAAGA AAGAAGTAAA AAAACTTCCC
    TCAGATGATG AAGATGATTT TTTCAAACCA CCCAAGCTTA CCGATGAAGA TTTCTCACCT
    TTTGGCAGTA AAGGAGGGCT TTTCAGTGGT GGAAAAGGAC TTTTTGATGA CGATGATGAT
    GAGGGTGATA TCTTTGCTGA TGTCCCCAGC AAGGAAGTCA AAAAAGCAGA ACCAAGTCCA
    TTGATAAATT CAGAGACAGC ACATTTGAAA AAGAAGCCAC CTGTTGGAGG GGTCTCTCTT
    TTCCCAGGTG GAGAAAATGT GATTGGGTCC TCAATCCTGG CAGAAAAGGA TAAAAATAAA
    TCTACAACTC CTACACAAGA TGTTGCTCCC AAGCCTCCAC CAAGCTCAAG TCTATTTGAC
    GATGATGATG GTGCACTGTT TGGGTCATCC AATTCTAATC ACACTACAAA TAAACCAGGA
    AAAAGTCAAC CAACAACTGA TCTGTTTGCA GATGATAATG ATTTGTTTCA AGACAAACCT
    GTTGCCCCTA TTCTAGCAAA GAACAAAGTG ACAAAAGAGA ATGAACCACC TAAGGAGAAC
    AATATAGAAA GCAAGAACCA TCAAGTGCCT GTTGAAAAAT CTAAACCCCT TGAATCTTCG
    ACAAAAAAAC AAACCAAAGG TCTCTTTTCT GATGAGGATG ATTCCGAGTC GGATCTGTTT
    TCCCCCATTC CAAGTGCCAG TAAGAGTAAA ACAGCCACCT TACCAGCAGC AAAGACAGGC
    AAAGCCTTGT CCCTGTTTGA TGATGAAGAT GAAGAGGATT TGTTCGCTTC TGTACCAGAT
    AAGAAGCAGA CAGCCCCTGT CACAAAGCCA GCACAGCAAA ACAAATTAAC TTCTGAAGAG
    AAGGCTCCAA AATCAGGCCT CTTCAGCAGT GATGAAGAGG ATCCCATCGG AACCTTAAAT
    CAAAAAAATA CAGTTGAAAA AACTCAGCCT GCAAAAGCTG CAGCAGTTGT ATCAAAAGGA
    ACGAGGGAAG AGCCATTGGG GAAGAAGTCA AGTTTGTTTG ATAATGACAC AGATGATCTT
    TTTGCAATCA CAAAAGAGAG TGAAAAGAAG CATAATCGTG TTTCACTGTT ATTTGAAGAT
    GACAATGAAG GATCTTTATT TTCTTCAGTA TCTACAACAG CTGCTTCTAC AAGCCAAGCT
    CCTGTAAACA CCATAGAAGA ATCTAAACTA CCAGATACTC ATGCACTCAT CTCTAAGAAG
    GAATCTTTGT TAACTAAACC AACAAAAACA GAGGGTCAAA AATCAGAGCC TGAACCACCA
    GTAAGTGTCT GTGAGAATAA GGAACCATTG CAGGAAAATA AAAAGGTGAA ACAAGCTGTT
    TTCTCAGAGC CAGGGGAGGA TAATCTCTTT GCAGCATCCC CTCCTGCCGA TAAACATGCA
    AAAATCAAAA GTAAAAATGT GCTAAGTCTC TTTGATGATG AAGACGAGGA CTTCGAAAAT
    CATACAGATA AAACTATTAC ACAAAGAATA CCTGGAAAGG TCTCCTCCGA GACGAGCTCC
    CACAAAAAGA GAACAGGCGT GTTCCAAGAT GAAGAACTTC TGTTCAGCCA AGAATTACAG
    AAAGACAATG ACCCTGATGT GGACCTGTTT GCTAGCGCTA AAAAGCCACC GTCTGAAAAA
    CCAAGCCATA CAAAATCATC AGCTGGAATG GGCTTGTTTG GGGACGATGA GGACGATGAT
    CTTTTTAGTA CAGCTAAACC AAAAAAGCCT CCGAAAGTGC CAGAGAAGAA ACCAGTGAAC
    ATAAAAGAAA AATCTGATGC CATTAAAGAA CCCCCCAAAG CAAGTGAAGT GAAAAAAGAT
    AATCTTTTGA GGAGAGAAAA TGCAGAGTGT CCCGCAGATT CTGTTTCAAT AAAATCAAAG
    GGTCCCTCCT CTCGGATTGG GAAGCTACAA GCAAATCTGC AGATTAACCC AGCTGCAATG
    TTACCAGGGG CAGTCCCCAA ATTAGCAGGA GTCAGGCCTG CCATTGTGGG CATTTCACCA
    CCACCCCTTG CAGATACGAT GGACAACAGC ACCTCGCCCA CTGCTAGTAC CGGCATACAA
    GGAGTCAGCT TTGAAAGCCC AGCACAGATT GATACTCTTC ACAGTGCAAA TAAGGCACGA
    CCCAGGAATG CCAGAAGGCG ACCACCTACA AGAATGGGCA GAAAACTTGC ATCACAAGAC
    TCTGGTGAAA CAATTGACGA CTCTGGAGTT TCACCCACCT CGCATACGGC TGCTACCACG
    CCTGAATTGG CACAATTGAA CTCGCACCAG CAATTCGATT CAACTGTTTC TAATATGAAG
    CAGTCACTGT CAGAGAATGA CGTACAGTCC AGAGTGAACA CAAAAGTTAC AACAAAACCT
    CTAACACCCA ACATAAATGA CTTGTTTGGG TCTGATCTTT TTGCAAAACG TTCATTACCC
    CCTGCTGATG CCTCAAAAAA GCAAGATGGT CCTCCTGATA ACTCATCTGC AAACAAACCT
    GGGCTGGGGA CTGGAACTAA ATCTCCTGCT TCAGTGATAG ATGAGCAGAA CAGCGATGAC
    GACCTTTTTC AGACAGTTAA ACAGAAGTCT GTAAAAATGC CCAAAGGGGC CTCATTAGTG
    GATAATGAAC CCAGCGATGA CCTTTTCAGA GTTTCAAAAT CCCAGAAGAA AGTGGATATA
    AAGACTGTCC AGCCTAAGGA AACAAAATCA AAAGCCAGCA ATATATTTGA GGATGACATA
    TTCGCAACTG AAGTAGTAAA ACCAGTAAAG AAAACAAAAG AAAAGAAACC ACCATCAGAA
    TCCAATCTCT TTGACGAGAA CGTGGATATA TTTGCTGATC TAACTCAAAA AGGTAAAGAG
    AAGAAAGCTA AGAAGAAGGT TGAGCCCAAA TCAATATTTG ATGATGATAT GGATGACATA
    TTTGCATCAG GAAGTGTAAA ACATACCAAG CAAAAAGCGA AACCCAGTGA AACTTCTGCG
    GAGGTGAAAT CTGAGAGCAA CGTTTCCAGC ATATTTGATG ACCCTTTAAA TGTTTTCAGC
    AAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017951133.1
    CDS411..4196
    Translation

    Target ORF information:

    RefSeq Version XM_018095644.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis family with sequence similarity 21 member A (fam21a), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018095644.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAACGGGC CGGCGAATGG TTCGGCAGAG ACGCCAGTGT GGGAGAGACC CTGGGGCTTG 
    GAGGAGATTA GGAGGAGCAG CCAGAACTGG TCCCTGGGTG CTGATGCGGG GCTCTTGAAT
    TTCCTCCGGG AGTTCTCTCA GCAAACCATA TCAAGGACAC ATGAAATAGA GAAACAACTG
    GATGGGCTCA TTCGTGAAAC AAAGGCCACA GACTGTCGTC TACACAATGT CTTTAATGAT
    TTTCTTATGT TATCTAACAC TCAGTTCATA GAAAATAGAG TGTATGATGA AGAAGTTGAA
    GATCATGTTG TTAAGGCAGA TGCTGGGAAC AAACCTGAAC AGGAGAAAAC TCGGGAGCAA
    AAGGAAGCAG AGCTGATTCC AAAAATACAG GAAGCTGTAA ACTATGGACT GCAAGTACTG
    GAAAATGCTT TTGAGCAACT GGATATTAAA GCAGGGAATT CTGACTCGGA GGAAGAAGAA
    GTTAATGAGC GTGTGGAGCC TATATTAGAG CCAAAGGATC TTTATATAGA CAGACCTCTG
    CCGCTTTTAA TTGGCTCCCA ACAGTTCATG CAGCAAGAAG ATGTTGGCCT TGGAGATCTC
    TCAAGTGAAG AGGGATCGGT GGACAATGAT CGGGGGAGCA TTATTGACAG TGAAGATGAA
    AAAGATGATG AGTTCATCCA GTGCAATTAT TTAAATCCGC TGCATTTGTT TTCAGCTGTT
    GCATCTCTGG AGGTGAAAAA AAAGAAAGAA AAAGATAAGA AAGAAGTAAA AAAACTTCCC
    TCAGATGATG AAGATGATTT TTTCAAACCA CCCAAGCTTA CCGATGAAGA TTTCTCACCT
    TTTGGCAGTA AAGGAGGGCT TTTCAGTGGT GGAAAAGGAC TTTTTGATGA CGATGATGAT
    GAGGGTGATA TCTTTGCTGA TGTCCCCAGC AAGGAAGTCA AAAAAGCAGA ACCAAGTCCA
    TTGATAAATT CAGAGACAGC ACATTTGAAA AAGAAGCCAC CTGTTGGAGG GGTCTCTCTT
    TTCCCAGGTG GAGAAAATGT GATTGGGTCC TCAATCCTGG CAGAAAAGGA TAAAAATAAA
    TCTACAACTC CTACACAAGA TGTTGCTCCC AAGCCTCCAC CAAGCTCAAG TCTATTTGAC
    GATGATGATG GTGCACTGTT TGGGTCATCC AATTCTAATC ACACTACAAA TAAACCAGGA
    AAAAGTCAAC CAACAACTGA TCTGTTTGCA GATGATAATG ATTTGTTTCA AGACAAACCT
    GTTGCCCCTA TTCTAGCAAA GAACAAAGTG ACAAAAGAGA ATGAACCACC TAAGGAGAAC
    AATATAGAAA GCAAGAACCA TCAAGTGCCT GTTGAAAAAT CTAAACCCCT TGAATCTTCG
    ACAAAAAAAC AAACCAAAGG TCTCTTTTCT GATGAGGATG ATTCCGAGTC GGATCTGTTT
    TCCCCCATTC CAAGTGCCAG TAAGAGTAAA ACAGCCACCT TACCAGCAGC AAAGACAGGC
    AAAGCCTTGT CCCTGTTTGA TGATGAAGAT GAAGAGGATT TGTTCGCTTC TGTACCAGAT
    AAGAAGCAGA CAGCCCCTGT CACAAAGCCA GCACAGCAAA ACAAATTAAC TTCTGAAGAG
    AAGGCTCCAA AATCAGGCCT CTTCAGCAGT GATGAAGAGG ATCCCATCGG AACCTTAAAT
    CAAAAAAATA CAGTTGAAAA AACTCAGCCT GCAAAAGCTG CAGCAGTTGT ATCAAAAGGA
    ACGAGGGAAG AGCCATTGGG GAAGAAGTCA AGTTTGTTTG ATAATGACAC AGATGATCTT
    TTTGCAATCA CAAAAGAGAG TGAAAAGAAG CATAATCGTG TTTCACTGTT ATTTGAAGAT
    GACAATGAAG GATCTTTATT TTCTTCAGTA TCTACAACAG CTGCTTCTAC AAGCCAAGCT
    CCTGTAAACA CCATAGAAGA ATCTAAACTA CCAGATACTC ATGCACTCAT CTCTAAGAAG
    GAATCTTTGT TAACTAAACC AACAAAAACA GAGGGTCAAA AATCAGAGCC TGAACCACCA
    GTAAGTGTCT GTGAGAATAA GGAACCATTG CAGGAAAATA AAAAGGTGAA ACAAGCTGTT
    TTCTCAGAGC CAGGGGAGGA TAATCTCTTT GCAGCATCCC CTCCTGCCGA TAAACATGCA
    AAAATCAAAA GTAAAAATGT GCTAAGTCTC TTTGATGATG AAGACGAGGA CTTCGAAAAT
    CATACAGATA AAACTATTAC ACAAAGAATA CCTGGAAAGG TCTCCTCCGA GACGAGCTCC
    CACAAAAAGA GAACAGGCGT GTTCCAAGAT GAAGAACTTC TGTTCAGCCA AGAATTACAG
    AAAGACAATG ACCCTGATGT GGACCTGTTT GCTAGCGCTA AAAAGCCACC GTCTGAAAAA
    CCAAGCCATA CAAAATCATC AGCTGGAATG GGCTTGTTTG GGGACGATGA GGACGATGAT
    CTTTTTAGTA CAGCTAAACC AAAAAAGCCT CCGAAAGTGC CAGAGAAGAA ACCAGTGAAC
    ATAAAAGAAA AATCTGATGC CATTAAAGAA CCCCCCAAAG CAAGTGAAGT GAAAAAAGAT
    AATCTTTTGA GGAGAGAAAA TGCAGAGTGT CCCGCAGATT CTGTTTCAAT AAAATCAAAG
    GGTCCCTCCT CTCGGATTGG GAAGCTACAA GCAAATCTGC AGATTAACCC AGCTGCAATG
    TTACCAGGGG CAGTCCCCAA ATTAGCAGGA GTCAGGCCTG CCATTGTGGG CATTTCACCA
    CCACCCCTTG CAGATACGAT GGACAACAGC ACCTCGCCCA CTGCTAGTAC CGGCATACAA
    GGAGTCAGCT TTGAAAGCCC AGCACAGATT GATACTCTTC ACAGTGCAAA TAAGGCACGA
    CCCAGGAATG CCAGAAGGCG ACCACCTACA AGAATGGGCA GAAAACTTGC ATCACAAGAC
    TCTGGTGAAA CAATTGACGA CTCTGGAGTT TCACCCACCT CGCATACGGC TGCTACCACG
    CCTGAATTGG CACAATTGAA CTCGCACCAG CAATTCGATT CAACTGTTTC TAATATGAAG
    CAGTCACTGT CAGAGAATGA CGTACAGTCC AGAGTGAACA CAAAAGTTAC AACAAAACCT
    CTAACACCCA ACATAAATGA CTTGTTTGGG TCTGATCTTT TTGCAAAACG TTCATTACCC
    CCTGCTGATG CCTCAAAAAA GCAAGATGGT CCTCCTGATA ACTCATCTGC AAACAAACCT
    GGGCTGGGGA CTGGAACTAA ATCTCCTGCT TCAGTGATAG ATGAGCAGAA CAGCGATGAC
    GACCTTTTTC AGACAGTTAA ACAGAAGTCT GTAAAAATGC CCAAAGGGGC CTCATTAGTG
    GATAATGAAC CCAGCGATGA CCTTTTCAGA GTTTCAAAAT CCCAGAAGAA AGTGGATATA
    AAGACTGTCC AGCCTAAGGA AACAAAATCA AAAGCCAGCA ATATATTTGA GGATGACATA
    TTCGCAACTG AAGTAGTAAA ACCAGTAAAG AAAACAAAAG AAAAGAAACC ACCATCAGAA
    TCCAATCTCT TTGACGAGAA CGTGGATATA TTTGCTGATC TAACTCAAAA AGGTAAAGAG
    AAGAAAGCTA AGAAGAAGGT TGAGCCCAAA TCAATATTTG ATGATGATAT GGATGACATA
    TTTGCATCAG GAAGTGTAAA ACATACCAAG CAAAAAGCGA AACCCAGTGA AACTTCTGCG
    GAGGTGAAAT CTGAGAGCAA CGTTTCCAGC ATATTTGATG ACCCTTTAAA TGTTTTCAGC
    AAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62873
    Clone ID Related Accession (Same CDS sequence) XM_031906385.1
    Accession Version XM_031906385.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3969bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product WASH complex subunit 2A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAACGGGC CGGCGAATGG TTCGGCAGAG ACGCCAGTGT GGGAGAGACC CTGGGGCTTG 
    GAGGAGATTA GGAGGAGCAG CCAGAACTGG TCCCTGGGTG CTGATGCGGG GCTCTTGAAT
    TTCCTCCGGG AGTTCTCTCA GCAAACCATA TCAAGGACAC ATGAAATAGA GAAACAACTG
    GATGGGCTCA TTCGTGAAAC AAAGGCCACA GACTGTCGTC TACACAATGT CTTTAATGAT
    TTTCTTATGT TATCTAACAC TCAGTTCATA GAAAATAGAG TGTATGATGA AGAAGTTGAA
    GATCATGTTG TTAAGGCAGA TGCTGGGAAC AAACCTGAAC AGGAGAAAAC TCGGGAGCAA
    AAGGAAGCAG AGCTGATTCC AAAAATACAG GAAGCTGTAA ACTATGGACT GCAAGTACTG
    GAAAATGCTT TTGAGCAACT GGATATTAAA GCAGGGAATT CTGACTCGGA GGAAGAAGAA
    GTTAATGAGC GTGTGGAGCC TATATTAGAG CCAAAGGATC TTTATATAGA CAGACCTCTG
    CCGCTTTTAA TTGGCTCCCA ACAGTTCATG CAGCAAGAAG ATGTTGGCCT TGGAGATCTC
    TCAAGTGAAG AGGGATCGGT GGACAATGAT CGGGGGAGCA TTATTGACAG TGAAGATGAA
    AAAGATGATG AGGAATCAGA AGATGATTTT GACAACAATT CTGAGGAGGA AAAGAAATTG
    CACTCCACAT TAAGTGATGA AGATGAAGAT AATGGCTCAG ACCTATTTGG TGATTCTGAC
    AAAGAAGAAG AGGAAGAAGA CAATAAAACT ACCAAAACAA GAACAAAATC TTTTGCTGAT
    GAACTGGCTG CTCGAATACA AAGCGATATC CCCAGGACAC AGGAATCCGA TCAATCATCT
    GTTGCATCTC TGGAGGTGAA AAAAAAGAAA GAAAAAGATA AGAAAGAAGT AAAAAAACTT
    CCCTCAGATG ATGAAGATGA TTTTTTCAAA CCACCCAAGC TTACCGATGA AGATTTCTCA
    CCTTTTGGCA GTAAAGGAGG GCTTTTCAGT GGTGGAAAAG GACTTTTTGA TGACGATGAT
    GATGAGGGTG ATATCTTTGC TGATGTCCCC AGCAAGGAAG TCAAAAAAGC AGAACCAAGT
    CCATTGATAA ATTCAGAGAC AGCACATTTG AAAAAGAAGC CACCTGTTGG AGGGGTCTCT
    CTTTTCCCAG GTGGAGAAAA TGTGATTGGG TCCTCAATCC TGGCAGAAAA GGATAAAAAT
    AAATCTACAA CTCCTACACA AGATGTTGCT CCCAAGCCTC CACCAAGCTC AAGTCTATTT
    GACGATGATG ATGGTGCACT GTTTGGGTCA TCCAATTCTA ATCACACTAC AAATAAACCA
    GGAAAAAGTC AACCAACAAC TGATCTGTTT GCAGATGATA ATGATTTGTT TCAAGACAAA
    CCTGTTGCCC CTATTCTAGC AAAGAACAAA GTGACAAAAG AGAATGAACC ACCTAAGGAG
    AACAATATAG AAAGCAAGAA CCATCAAGTG CCTGTTGAAA AATCTAAACC CCTTGAATCT
    TCGACAAAAA AACAAACCAA AGGTCTCTTT TCTGATGAGG ATGATTCCGA GTCGGATCTG
    TTTTCCCCCA TTCCAAGTGC CAGTAAGAGT AAAACAGCCA CCTTACCAGC AGCAAAGACA
    GGCAAAGCCT TGTCCCTGTT TGATGATGAA GATGAAGAGG ATTTGTTCGC TTCTGTACCA
    GATAAGAAGC AGACAGCCCC TGTCACAAAG CCAGCACAGC AAAACAAATT AACTTCTGAA
    GAGAAGGCTC CAAAATCAGG CCTCTTCAGC AGTGATGAAG AGGATCCCAT CGGAACCTTA
    AATCAAAAAA ATACAGTTGA AAAAACTCAG CCTGCAAAAG CTGCAGCAGT TGTATCAAAA
    GGAACGAGGG AAGAGCCATT GGGGAAGAAG TCAAGTTTGT TTGATAATGA CACAGATGAT
    CTTTTTGCAA TCACAAAAGA GAGTGAAAAG AAGCATAATC GTGTTTCACT GTTATTTGAA
    GATGACAATG AAGGATCTTT ATTTTCTTCA GTATCTACAA CAGCTGCTTC TACAAGCCAA
    GCTCCTGTAA ACACCATAGA AGAATCTAAA CTACCAGATA CTCATGCACT CATCTCTAAG
    AAGGAATCTT TGTTAACTAA ACCAACAAAA ACAGAGGGTC AAAAATCAGA GCCTGAACCA
    CCAGTAAGTG TCTGTGAGAA TAAGGAACCA TTGCAGGAAA ATAAAAAGGT GAAACAAGCT
    GTTTTCTCAG AGCCAGGGGA GGATAATCTC TTTGCAGCAT CCCCTCCTGC CGATAAACAT
    GCAAAAATCA AAAGTAAAAA TGTGCTAAGT CTCTTTGATG ATGAAGACGA GGACTTCGAA
    AATCATACAG ATAAAACTAT TACACAAAGA ATACCTGGAA AGGTCTCCTC CGAGACGAGC
    TCCCACAAAA AGAGAACAGG CGTGTTCCAA GATGAAGAAC TTCTGTTCAG CCAAGAATTA
    CAGAAAGACA ATGACCCTGA TGTGGACCTG TTTGCTAGCG CTAAAAAGCC ACCGTCTGAA
    AAACCAAGCC ATACAAAATC ATCAGCTGGA ATGGGCTTGT TTGGGGACGA TGAGGACGAT
    GATCTTTTTA GTACAGCTAA ACCAAAAAAG CCTCCGAAAG TGCCAGAGAA GAAACCAGTG
    AACATAAAAG AAAAATCTGA TGCCATTAAA GAACCCCCCA AAGCAAGTGA AGTGAAAAAA
    GATAATCTTT TGAGGAGAGA AAATGCAGAG TGTCCCGCAG ATTCTGTTTC AATAAAATCA
    AAGGGTCCCT CCTCTCGGAT TGGGAAGCTA CAAGCAAATC TGCAGATTAA CCCAGCTGCA
    ATGTTACCAG GGGCAGTCCC CAAATTAGCA GGAGTCAGGC CTGCCATTGT GGGCATTTCA
    CCACCACCCC TTGCAGATAC GATGGACAAC AGCACCTCGC CCACTGCTAG TACCGGCATA
    CAAGGAGTCA GCTTTGAAAG CCCAGCACAG ATTGATACTC TTCACAGTGC AAATAAGGCA
    CGACCCAGGA ATGCCAGAAG GCGACCACCT ACAAGAATGG GCAGAAAACT TGCATCACAA
    GACTCTGGTG AAACAATTGA CGACTCTGGA GTTTCACCCA CCTCGCATAC GGCTGCTACC
    ACGCCTGAAT TGGCACAATT GAACTCGCAC CAGCAATTCG ATTCAACTGT TTCTAATATG
    AAGCAGTCAC TGTCAGAGAA TGACGTACAG TCCAGAGTGA ACACAAAAGT TACAACAAAA
    CCTCTAACAC CCAACATAAA TGACTTGTTT GGGTCTGATC TTTTTGCAAA ACGTTCATTA
    CCCCCTGCTG ATGCCTCAAA AAAGCAAGAT GGTCCTCCTG ATAACTCATC TGCAAACAAA
    CCTGGGCTGG GGACTGGAAC TAAATCTCCT GCTTCAGTGA TAGATGAGCA GAACAGCGAT
    GACGACCTTT TTCAGACAGT TAAACAGAAG TCTGTAAAAA TGCCCAAAGG GGCCTCATTA
    GTGGATAATG AACCCAGCGA TGACCTTTTC AGAGTTTCAA AATCCCAGAA GAAAGTGGAT
    ATAAAGACTG TCCAGCCTAA GGAAACAAAA TCAAAAGCCA GCAATATATT TGAGGATGAC
    ATATTCGCAA CTGAAGTAGT AAAACCAGTA AAGAAAACAA AAGAAAAGAA ACCACCATCA
    GAATCCAATC TCTTTGACGA GAACGTGGAT ATATTTGCTG ATCTAACTCA AAAAGGTAAA
    GAGAAGAAAG CTAAGAAGAA GGTTGAGCCC AAATCAATAT TTGATGATGA TATGGATGAC
    ATATTTGCAT CAGGAAGTGT AAAACATACC AAGCAAAAAG CGAAACCCAG TGAAACTTCT
    GCGGAGGTGA AATCTGAGAG CAACGTTTCC AGCATATTTG ATGACCCTTT AAATGTTTTC
    AGCAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031762245.1
    CDS144..4112
    Translation

    Target ORF information:

    RefSeq Version XM_031906385.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis WASH complex subunit 2A (washc2a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031906385.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAACGGGC CGGCGAATGG TTCGGCAGAG ACGCCAGTGT GGGAGAGACC CTGGGGCTTG 
    GAGGAGATTA GGAGGAGCAG CCAGAACTGG TCCCTGGGTG CTGATGCGGG GCTCTTGAAT
    TTCCTCCGGG AGTTCTCTCA GCAAACCATA TCAAGGACAC ATGAAATAGA GAAACAACTG
    GATGGGCTCA TTCGTGAAAC AAAGGCCACA GACTGTCGTC TACACAATGT CTTTAATGAT
    TTTCTTATGT TATCTAACAC TCAGTTCATA GAAAATAGAG TGTATGATGA AGAAGTTGAA
    GATCATGTTG TTAAGGCAGA TGCTGGGAAC AAACCTGAAC AGGAGAAAAC TCGGGAGCAA
    AAGGAAGCAG AGCTGATTCC AAAAATACAG GAAGCTGTAA ACTATGGACT GCAAGTACTG
    GAAAATGCTT TTGAGCAACT GGATATTAAA GCAGGGAATT CTGACTCGGA GGAAGAAGAA
    GTTAATGAGC GTGTGGAGCC TATATTAGAG CCAAAGGATC TTTATATAGA CAGACCTCTG
    CCGCTTTTAA TTGGCTCCCA ACAGTTCATG CAGCAAGAAG ATGTTGGCCT TGGAGATCTC
    TCAAGTGAAG AGGGATCGGT GGACAATGAT CGGGGGAGCA TTATTGACAG TGAAGATGAA
    AAAGATGATG AGGAATCAGA AGATGATTTT GACAACAATT CTGAGGAGGA AAAGAAATTG
    CACTCCACAT TAAGTGATGA AGATGAAGAT AATGGCTCAG ACCTATTTGG TGATTCTGAC
    AAAGAAGAAG AGGAAGAAGA CAATAAAACT ACCAAAACAA GAACAAAATC TTTTGCTGAT
    GAACTGGCTG CTCGAATACA AAGCGATATC CCCAGGACAC AGGAATCCGA TCAATCATCT
    GTTGCATCTC TGGAGGTGAA AAAAAAGAAA GAAAAAGATA AGAAAGAAGT AAAAAAACTT
    CCCTCAGATG ATGAAGATGA TTTTTTCAAA CCACCCAAGC TTACCGATGA AGATTTCTCA
    CCTTTTGGCA GTAAAGGAGG GCTTTTCAGT GGTGGAAAAG GACTTTTTGA TGACGATGAT
    GATGAGGGTG ATATCTTTGC TGATGTCCCC AGCAAGGAAG TCAAAAAAGC AGAACCAAGT
    CCATTGATAA ATTCAGAGAC AGCACATTTG AAAAAGAAGC CACCTGTTGG AGGGGTCTCT
    CTTTTCCCAG GTGGAGAAAA TGTGATTGGG TCCTCAATCC TGGCAGAAAA GGATAAAAAT
    AAATCTACAA CTCCTACACA AGATGTTGCT CCCAAGCCTC CACCAAGCTC AAGTCTATTT
    GACGATGATG ATGGTGCACT GTTTGGGTCA TCCAATTCTA ATCACACTAC AAATAAACCA
    GGAAAAAGTC AACCAACAAC TGATCTGTTT GCAGATGATA ATGATTTGTT TCAAGACAAA
    CCTGTTGCCC CTATTCTAGC AAAGAACAAA GTGACAAAAG AGAATGAACC ACCTAAGGAG
    AACAATATAG AAAGCAAGAA CCATCAAGTG CCTGTTGAAA AATCTAAACC CCTTGAATCT
    TCGACAAAAA AACAAACCAA AGGTCTCTTT TCTGATGAGG ATGATTCCGA GTCGGATCTG
    TTTTCCCCCA TTCCAAGTGC CAGTAAGAGT AAAACAGCCA CCTTACCAGC AGCAAAGACA
    GGCAAAGCCT TGTCCCTGTT TGATGATGAA GATGAAGAGG ATTTGTTCGC TTCTGTACCA
    GATAAGAAGC AGACAGCCCC TGTCACAAAG CCAGCACAGC AAAACAAATT AACTTCTGAA
    GAGAAGGCTC CAAAATCAGG CCTCTTCAGC AGTGATGAAG AGGATCCCAT CGGAACCTTA
    AATCAAAAAA ATACAGTTGA AAAAACTCAG CCTGCAAAAG CTGCAGCAGT TGTATCAAAA
    GGAACGAGGG AAGAGCCATT GGGGAAGAAG TCAAGTTTGT TTGATAATGA CACAGATGAT
    CTTTTTGCAA TCACAAAAGA GAGTGAAAAG AAGCATAATC GTGTTTCACT GTTATTTGAA
    GATGACAATG AAGGATCTTT ATTTTCTTCA GTATCTACAA CAGCTGCTTC TACAAGCCAA
    GCTCCTGTAA ACACCATAGA AGAATCTAAA CTACCAGATA CTCATGCACT CATCTCTAAG
    AAGGAATCTT TGTTAACTAA ACCAACAAAA ACAGAGGGTC AAAAATCAGA GCCTGAACCA
    CCAGTAAGTG TCTGTGAGAA TAAGGAACCA TTGCAGGAAA ATAAAAAGGT GAAACAAGCT
    GTTTTCTCAG AGCCAGGGGA GGATAATCTC TTTGCAGCAT CCCCTCCTGC CGATAAACAT
    GCAAAAATCA AAAGTAAAAA TGTGCTAAGT CTCTTTGATG ATGAAGACGA GGACTTCGAA
    AATCATACAG ATAAAACTAT TACACAAAGA ATACCTGGAA AGGTCTCCTC CGAGACGAGC
    TCCCACAAAA AGAGAACAGG CGTGTTCCAA GATGAAGAAC TTCTGTTCAG CCAAGAATTA
    CAGAAAGACA ATGACCCTGA TGTGGACCTG TTTGCTAGCG CTAAAAAGCC ACCGTCTGAA
    AAACCAAGCC ATACAAAATC ATCAGCTGGA ATGGGCTTGT TTGGGGACGA TGAGGACGAT
    GATCTTTTTA GTACAGCTAA ACCAAAAAAG CCTCCGAAAG TGCCAGAGAA GAAACCAGTG
    AACATAAAAG AAAAATCTGA TGCCATTAAA GAACCCCCCA AAGCAAGTGA AGTGAAAAAA
    GATAATCTTT TGAGGAGAGA AAATGCAGAG TGTCCCGCAG ATTCTGTTTC AATAAAATCA
    AAGGGTCCCT CCTCTCGGAT TGGGAAGCTA CAAGCAAATC TGCAGATTAA CCCAGCTGCA
    ATGTTACCAG GGGCAGTCCC CAAATTAGCA GGAGTCAGGC CTGCCATTGT GGGCATTTCA
    CCACCACCCC TTGCAGATAC GATGGACAAC AGCACCTCGC CCACTGCTAG TACCGGCATA
    CAAGGAGTCA GCTTTGAAAG CCCAGCACAG ATTGATACTC TTCACAGTGC AAATAAGGCA
    CGACCCAGGA ATGCCAGAAG GCGACCACCT ACAAGAATGG GCAGAAAACT TGCATCACAA
    GACTCTGGTG AAACAATTGA CGACTCTGGA GTTTCACCCA CCTCGCATAC GGCTGCTACC
    ACGCCTGAAT TGGCACAATT GAACTCGCAC CAGCAATTCG ATTCAACTGT TTCTAATATG
    AAGCAGTCAC TGTCAGAGAA TGACGTACAG TCCAGAGTGA ACACAAAAGT TACAACAAAA
    CCTCTAACAC CCAACATAAA TGACTTGTTT GGGTCTGATC TTTTTGCAAA ACGTTCATTA
    CCCCCTGCTG ATGCCTCAAA AAAGCAAGAT GGTCCTCCTG ATAACTCATC TGCAAACAAA
    CCTGGGCTGG GGACTGGAAC TAAATCTCCT GCTTCAGTGA TAGATGAGCA GAACAGCGAT
    GACGACCTTT TTCAGACAGT TAAACAGAAG TCTGTAAAAA TGCCCAAAGG GGCCTCATTA
    GTGGATAATG AACCCAGCGA TGACCTTTTC AGAGTTTCAA AATCCCAGAA GAAAGTGGAT
    ATAAAGACTG TCCAGCCTAA GGAAACAAAA TCAAAAGCCA GCAATATATT TGAGGATGAC
    ATATTCGCAA CTGAAGTAGT AAAACCAGTA AAGAAAACAA AAGAAAAGAA ACCACCATCA
    GAATCCAATC TCTTTGACGA GAACGTGGAT ATATTTGCTG ATCTAACTCA AAAAGGTAAA
    GAGAAGAAAG CTAAGAAGAA GGTTGAGCCC AAATCAATAT TTGATGATGA TATGGATGAC
    ATATTTGCAT CAGGAAGTGT AAAACATACC AAGCAAAAAG CGAAACCCAG TGAAACTTCT
    GCGGAGGTGA AATCTGAGAG CAACGTTTCC AGCATATTTG ATGACCCTTT AAATGTTTTC
    AGCAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62874
    Clone ID Related Accession (Same CDS sequence) XM_031906386.1
    Accession Version XM_031906386.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3924bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product WASH complex subunit 2A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAACGGGC CGGCGAATGG TTCGGCAGAG ACGCCAGTGT GGGAGAGACC CTGGGGCTTG 
    GAGGAGATTA GGAGGAGCAG CCAGAACTGG TCCCTGGGTG CTGATGCGGG GCTCTTGAAT
    TTCCTCCGGG AGTTCTCTCA GCAAACCATA TCAAGGACAC ATGAAATAGA GAAACAACTG
    GATGGGCTCA TTCGTGAAAC AAAGGCCACA GACTGTCGTC TACACAATGT CTTTAATGAT
    TTTCTTATGT TATCTAACAC TCAGTTCATA GAAAATAGAG TGTATGATGA AGAAGTTGAA
    GATCATGTTG TTAAGGCAGA TGCTGGGAAC AAACCTGAAC AGGAGAAAAC TCGGGAGCAA
    AAGGAAGCAG AGCTGATTCC AAAAATACAG GAAGCTGTAA ACTATGGACT GCAAGTACTG
    GAAAATGCTT TTGAGCAACT GGATATTAAA GCAGGGAATT CTGACTCGGA GGAAGAAGAA
    GTTAATGAGC GTGTGGAGCC TATATTAGAG CCAAAGGATC TTTATATAGA CAGACCTCTG
    CCGCTTTTAA TTGGCTCCCA ACAGTTCATG CAGCAAGAAG ATGTTGGCCT TGGAGATCTC
    TCAAGTGAAG AGGGATCGGT GGACAATGAT CGGGGGAGCA TTATTGACAG TGAAGATGAA
    AAAGATGATG AGGAATCAGA AGATGATTTT GACAACAATT CTGAGGAGGA AAAGAAATTG
    CACTCCACAT TAAGTGATGA AGATGAAGAT AATGGCTCAG ACCTATTTGG TGATTCTGAC
    AAAGAAGAAG AGGAAGAAGA CAATAAAACT ACCAAAACAA GAACAAAATC TTTTGCTGAT
    GAACTGGCTG CTCGAATACA AAGCGATATC CCCAGGACAC AGGAATCCGA TCAATCATCT
    GTTGCATCTC TGGAGGTGAA AAAAAAGAAA GAAAAAGATA AGAAAGAAGT AAAAAAACTT
    CCCTCAGATG ATGAAGATGA TTTTTTCAAA CCACCCAAGC TTACCGATGA AGATTTCTCA
    CCTTTTGGCA GTAAAGGAGG GCTTTTCAGT GGTGGAAAAG GACTTTTTGA TGACGATGAT
    GATGAGGGTG ATATCTTTGC TGATGTCCCC AGCAAGGAAG TCAAAAAAGC AGAACCAAGT
    CCATTGATAA ATTCAGAGAC AGCACATTTG AAAAAGAAGC CACCTGTTGG AGGGGTCTCT
    CTTTTCCCAG GTGGAGAAAA TGTGATTGGG TCCTCAATCC TGGCAGAAAA GGATAAAAAT
    AAATCTACAA CTCCTACACA AGATGTTGCT CCCAAGCCTC CACCAAGCTC AAGTCTATTT
    GACGATGATG ATGGTGCACT GTTTGGGTCA TCCAATTCTA ATCACACTAC AAATAAACCA
    GGAAAAAGTC AACCAACAAC TGATCTGTTT GCAGATGATA ATGATTTGTT TCAAGACAAA
    CCTGTTGCCC CTATTCTAGC AAAGAACAAA GTGACAAAAG AGAATGAACC ACCTAAGGAG
    AACAATATAG AAAGCAAGAA CCATCAAGTG CCTGTTGAAA AATCTAAACC CCTTGAATCT
    TCGACAAAAA AACAAACCAA AGGTCTCTTT TCTGATGAGG ATGATTCCGA GTCGGATCTG
    TTTTCCCCCA TTCCAAGTGC CAGTAAGAGT AAAACAGCCA CCTTACCAGC AGCAAAGACA
    GGCAAAGCCT TGTCCCTGTT TGATGATGAA GATGAAGAGG ATTTGTTCGC TTCTGTACCA
    GATAAGAAGC AGACAGCCCC TGTCACAAAG CCAGCACAGC AAAACAAATT AACTTCTGAA
    GAGAAGGCTC CAAAATCAGG CCTCTTCAGC AGTGATGAAG AGGATCCCAT CGGAACCTTA
    AATCAAAAAA ATACAGTTGA AAAAACTCAG CCTGCAAAAG CTGCAGCAGT TGTATCAAAA
    GGAACGAGGG AAGAGCCATT GGGGAAGAAG TCAAGTTTGT TTGATAATGA CACAGATGAT
    CTTTTTGCAA TCACAAAAGA GAGTGAAAAG AAGCATAATC GTGTTTCACT GTTATTTGAA
    GATGACAATG AAGGATCTTT ATTTTCTTCA GTATCTACAA CAGCTGCTTC TACAAGCCAA
    GCTCCTGTAA ACACCATAGA AGAATCTAAA CTACCAGATA CTCATGCACT CATCTCTAAG
    AAGGAATCTT TGTTAACTAA ACCAACAAAA ACAGAGGGTC AAAAATCAGA GCCTGAACCA
    CCAGTGAAAC AAGCTGTTTT CTCAGAGCCA GGGGAGGATA ATCTCTTTGC AGCATCCCCT
    CCTGCCGATA AACATGCAAA AATCAAAAGT AAAAATGTGC TAAGTCTCTT TGATGATGAA
    GACGAGGACT TCGAAAATCA TACAGATAAA ACTATTACAC AAAGAATACC TGGAAAGGTC
    TCCTCCGAGA CGAGCTCCCA CAAAAAGAGA ACAGGCGTGT TCCAAGATGA AGAACTTCTG
    TTCAGCCAAG AATTACAGAA AGACAATGAC CCTGATGTGG ACCTGTTTGC TAGCGCTAAA
    AAGCCACCGT CTGAAAAACC AAGCCATACA AAATCATCAG CTGGAATGGG CTTGTTTGGG
    GACGATGAGG ACGATGATCT TTTTAGTACA GCTAAACCAA AAAAGCCTCC GAAAGTGCCA
    GAGAAGAAAC CAGTGAACAT AAAAGAAAAA TCTGATGCCA TTAAAGAACC CCCCAAAGCA
    AGTGAAGTGA AAAAAGATAA TCTTTTGAGG AGAGAAAATG CAGAGTGTCC CGCAGATTCT
    GTTTCAATAA AATCAAAGGG TCCCTCCTCT CGGATTGGGA AGCTACAAGC AAATCTGCAG
    ATTAACCCAG CTGCAATGTT ACCAGGGGCA GTCCCCAAAT TAGCAGGAGT CAGGCCTGCC
    ATTGTGGGCA TTTCACCACC ACCCCTTGCA GATACGATGG ACAACAGCAC CTCGCCCACT
    GCTAGTACCG GCATACAAGG AGTCAGCTTT GAAAGCCCAG CACAGATTGA TACTCTTCAC
    AGTGCAAATA AGGCACGACC CAGGAATGCC AGAAGGCGAC CACCTACAAG AATGGGCAGA
    AAACTTGCAT CACAAGACTC TGGTGAAACA ATTGACGACT CTGGAGTTTC ACCCACCTCG
    CATACGGCTG CTACCACGCC TGAATTGGCA CAATTGAACT CGCACCAGCA ATTCGATTCA
    ACTGTTTCTA ATATGAAGCA GTCACTGTCA GAGAATGACG TACAGTCCAG AGTGAACACA
    AAAGTTACAA CAAAACCTCT AACACCCAAC ATAAATGACT TGTTTGGGTC TGATCTTTTT
    GCAAAACGTT CATTACCCCC TGCTGATGCC TCAAAAAAGC AAGATGGTCC TCCTGATAAC
    TCATCTGCAA ACAAACCTGG GCTGGGGACT GGAACTAAAT CTCCTGCTTC AGTGATAGAT
    GAGCAGAACA GCGATGACGA CCTTTTTCAG ACAGTTAAAC AGAAGTCTGT AAAAATGCCC
    AAAGGGGCCT CATTAGTGGA TAATGAACCC AGCGATGACC TTTTCAGAGT TTCAAAATCC
    CAGAAGAAAG TGGATATAAA GACTGTCCAG CCTAAGGAAA CAAAATCAAA AGCCAGCAAT
    ATATTTGAGG ATGACATATT CGCAACTGAA GTAGTAAAAC CAGTAAAGAA AACAAAAGAA
    AAGAAACCAC CATCAGAATC CAATCTCTTT GACGAGAACG TGGATATATT TGCTGATCTA
    ACTCAAAAAG GTAAAGAGAA GAAAGCTAAG AAGAAGGTTG AGCCCAAATC AATATTTGAT
    GATGATATGG ATGACATATT TGCATCAGGA AGTGTAAAAC ATACCAAGCA AAAAGCGAAA
    CCCAGTGAAA CTTCTGCGGA GGTGAAATCT GAGAGCAACG TTTCCAGCAT ATTTGATGAC
    CCTTTAAATG TTTTCAGCAA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031762246.1
    CDS144..4067
    Translation

    Target ORF information:

    RefSeq Version XM_031906386.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis WASH complex subunit 2A (washc2a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031906386.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAACGGGC CGGCGAATGG TTCGGCAGAG ACGCCAGTGT GGGAGAGACC CTGGGGCTTG 
    GAGGAGATTA GGAGGAGCAG CCAGAACTGG TCCCTGGGTG CTGATGCGGG GCTCTTGAAT
    TTCCTCCGGG AGTTCTCTCA GCAAACCATA TCAAGGACAC ATGAAATAGA GAAACAACTG
    GATGGGCTCA TTCGTGAAAC AAAGGCCACA GACTGTCGTC TACACAATGT CTTTAATGAT
    TTTCTTATGT TATCTAACAC TCAGTTCATA GAAAATAGAG TGTATGATGA AGAAGTTGAA
    GATCATGTTG TTAAGGCAGA TGCTGGGAAC AAACCTGAAC AGGAGAAAAC TCGGGAGCAA
    AAGGAAGCAG AGCTGATTCC AAAAATACAG GAAGCTGTAA ACTATGGACT GCAAGTACTG
    GAAAATGCTT TTGAGCAACT GGATATTAAA GCAGGGAATT CTGACTCGGA GGAAGAAGAA
    GTTAATGAGC GTGTGGAGCC TATATTAGAG CCAAAGGATC TTTATATAGA CAGACCTCTG
    CCGCTTTTAA TTGGCTCCCA ACAGTTCATG CAGCAAGAAG ATGTTGGCCT TGGAGATCTC
    TCAAGTGAAG AGGGATCGGT GGACAATGAT CGGGGGAGCA TTATTGACAG TGAAGATGAA
    AAAGATGATG AGGAATCAGA AGATGATTTT GACAACAATT CTGAGGAGGA AAAGAAATTG
    CACTCCACAT TAAGTGATGA AGATGAAGAT AATGGCTCAG ACCTATTTGG TGATTCTGAC
    AAAGAAGAAG AGGAAGAAGA CAATAAAACT ACCAAAACAA GAACAAAATC TTTTGCTGAT
    GAACTGGCTG CTCGAATACA AAGCGATATC CCCAGGACAC AGGAATCCGA TCAATCATCT
    GTTGCATCTC TGGAGGTGAA AAAAAAGAAA GAAAAAGATA AGAAAGAAGT AAAAAAACTT
    CCCTCAGATG ATGAAGATGA TTTTTTCAAA CCACCCAAGC TTACCGATGA AGATTTCTCA
    CCTTTTGGCA GTAAAGGAGG GCTTTTCAGT GGTGGAAAAG GACTTTTTGA TGACGATGAT
    GATGAGGGTG ATATCTTTGC TGATGTCCCC AGCAAGGAAG TCAAAAAAGC AGAACCAAGT
    CCATTGATAA ATTCAGAGAC AGCACATTTG AAAAAGAAGC CACCTGTTGG AGGGGTCTCT
    CTTTTCCCAG GTGGAGAAAA TGTGATTGGG TCCTCAATCC TGGCAGAAAA GGATAAAAAT
    AAATCTACAA CTCCTACACA AGATGTTGCT CCCAAGCCTC CACCAAGCTC AAGTCTATTT
    GACGATGATG ATGGTGCACT GTTTGGGTCA TCCAATTCTA ATCACACTAC AAATAAACCA
    GGAAAAAGTC AACCAACAAC TGATCTGTTT GCAGATGATA ATGATTTGTT TCAAGACAAA
    CCTGTTGCCC CTATTCTAGC AAAGAACAAA GTGACAAAAG AGAATGAACC ACCTAAGGAG
    AACAATATAG AAAGCAAGAA CCATCAAGTG CCTGTTGAAA AATCTAAACC CCTTGAATCT
    TCGACAAAAA AACAAACCAA AGGTCTCTTT TCTGATGAGG ATGATTCCGA GTCGGATCTG
    TTTTCCCCCA TTCCAAGTGC CAGTAAGAGT AAAACAGCCA CCTTACCAGC AGCAAAGACA
    GGCAAAGCCT TGTCCCTGTT TGATGATGAA GATGAAGAGG ATTTGTTCGC TTCTGTACCA
    GATAAGAAGC AGACAGCCCC TGTCACAAAG CCAGCACAGC AAAACAAATT AACTTCTGAA
    GAGAAGGCTC CAAAATCAGG CCTCTTCAGC AGTGATGAAG AGGATCCCAT CGGAACCTTA
    AATCAAAAAA ATACAGTTGA AAAAACTCAG CCTGCAAAAG CTGCAGCAGT TGTATCAAAA
    GGAACGAGGG AAGAGCCATT GGGGAAGAAG TCAAGTTTGT TTGATAATGA CACAGATGAT
    CTTTTTGCAA TCACAAAAGA GAGTGAAAAG AAGCATAATC GTGTTTCACT GTTATTTGAA
    GATGACAATG AAGGATCTTT ATTTTCTTCA GTATCTACAA CAGCTGCTTC TACAAGCCAA
    GCTCCTGTAA ACACCATAGA AGAATCTAAA CTACCAGATA CTCATGCACT CATCTCTAAG
    AAGGAATCTT TGTTAACTAA ACCAACAAAA ACAGAGGGTC AAAAATCAGA GCCTGAACCA
    CCAGTGAAAC AAGCTGTTTT CTCAGAGCCA GGGGAGGATA ATCTCTTTGC AGCATCCCCT
    CCTGCCGATA AACATGCAAA AATCAAAAGT AAAAATGTGC TAAGTCTCTT TGATGATGAA
    GACGAGGACT TCGAAAATCA TACAGATAAA ACTATTACAC AAAGAATACC TGGAAAGGTC
    TCCTCCGAGA CGAGCTCCCA CAAAAAGAGA ACAGGCGTGT TCCAAGATGA AGAACTTCTG
    TTCAGCCAAG AATTACAGAA AGACAATGAC CCTGATGTGG ACCTGTTTGC TAGCGCTAAA
    AAGCCACCGT CTGAAAAACC AAGCCATACA AAATCATCAG CTGGAATGGG CTTGTTTGGG
    GACGATGAGG ACGATGATCT TTTTAGTACA GCTAAACCAA AAAAGCCTCC GAAAGTGCCA
    GAGAAGAAAC CAGTGAACAT AAAAGAAAAA TCTGATGCCA TTAAAGAACC CCCCAAAGCA
    AGTGAAGTGA AAAAAGATAA TCTTTTGAGG AGAGAAAATG CAGAGTGTCC CGCAGATTCT
    GTTTCAATAA AATCAAAGGG TCCCTCCTCT CGGATTGGGA AGCTACAAGC AAATCTGCAG
    ATTAACCCAG CTGCAATGTT ACCAGGGGCA GTCCCCAAAT TAGCAGGAGT CAGGCCTGCC
    ATTGTGGGCA TTTCACCACC ACCCCTTGCA GATACGATGG ACAACAGCAC CTCGCCCACT
    GCTAGTACCG GCATACAAGG AGTCAGCTTT GAAAGCCCAG CACAGATTGA TACTCTTCAC
    AGTGCAAATA AGGCACGACC CAGGAATGCC AGAAGGCGAC CACCTACAAG AATGGGCAGA
    AAACTTGCAT CACAAGACTC TGGTGAAACA ATTGACGACT CTGGAGTTTC ACCCACCTCG
    CATACGGCTG CTACCACGCC TGAATTGGCA CAATTGAACT CGCACCAGCA ATTCGATTCA
    ACTGTTTCTA ATATGAAGCA GTCACTGTCA GAGAATGACG TACAGTCCAG AGTGAACACA
    AAAGTTACAA CAAAACCTCT AACACCCAAC ATAAATGACT TGTTTGGGTC TGATCTTTTT
    GCAAAACGTT CATTACCCCC TGCTGATGCC TCAAAAAAGC AAGATGGTCC TCCTGATAAC
    TCATCTGCAA ACAAACCTGG GCTGGGGACT GGAACTAAAT CTCCTGCTTC AGTGATAGAT
    GAGCAGAACA GCGATGACGA CCTTTTTCAG ACAGTTAAAC AGAAGTCTGT AAAAATGCCC
    AAAGGGGCCT CATTAGTGGA TAATGAACCC AGCGATGACC TTTTCAGAGT TTCAAAATCC
    CAGAAGAAAG TGGATATAAA GACTGTCCAG CCTAAGGAAA CAAAATCAAA AGCCAGCAAT
    ATATTTGAGG ATGACATATT CGCAACTGAA GTAGTAAAAC CAGTAAAGAA AACAAAAGAA
    AAGAAACCAC CATCAGAATC CAATCTCTTT GACGAGAACG TGGATATATT TGCTGATCTA
    ACTCAAAAAG GTAAAGAGAA GAAAGCTAAG AAGAAGGTTG AGCCCAAATC AATATTTGAT
    GATGATATGG ATGACATATT TGCATCAGGA AGTGTAAAAC ATACCAAGCA AAAAGCGAAA
    CCCAGTGAAA CTTCTGCGGA GGTGAAATCT GAGAGCAACG TTTCCAGCAT ATTTGATGAC
    CCTTTAAATG TTTTCAGCAA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62875
    Clone ID Related Accession (Same CDS sequence) XM_031906387.1
    Accession Version XM_031906387.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3906bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product WASH complex subunit 2C isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAACGGGC CGGCGAATGG TTCGGCAGAG ACGCCAGTGT GGGAGAGACC CTGGGGCTTG 
    GAGGAGATTA GGAGGAGCAG CCAGAACTGG TCCCTGGGTG CTGATGCGGG GCTCTTGAAT
    TTCCTCCGGG AGTTCTCTCA GCAAACCATA TCAAGGACAC ATGAAATAGA GAAACAACTG
    GATGGGCTCA TTCGTGAAAC AAAGGCCACA GACTGTCGTC TACACAATGT CTTTAATGAT
    TTTCTTATGT TATCTAACAC TCAGTTCATA GAAAATAGAG TGTATGATGA AGAAGTTGAA
    GATCATGTTG TTAAGGCAGA TGCTGGGAAC AAACCTGAAC AGGAGAAAAC TCGGGAGCAA
    AAGGAAGCAG AGCTGATTCC AAAAATACAG GAAGCTGTAA ACTATGGACT GCAAGTACTG
    GAAAATGCTT TTGAGCAACT GGATATTAAA GCAGGGAATT CTGACTCGGA GGAAGAAGAA
    GTTAATGAGC GTGTGGAGCC TATATTAGAG CCAAAGGATC TTTATATAGA CAGACCTCTG
    CCGCTTTTAA TTGGCTCCCA ACAGTTCATG CAGCAAGAAG ATGTTGGCCT TGGAGATCTC
    TCAAGTGAAG AGGGATCGGT GGACAATGAT CGGGGGAGCA TTATTGACAG TGAAGATGAA
    AAAGATGATG AGGAATCAGA AGATGATTTT GACAACAATT CTGAGGAGGA AAAGAAATTG
    CACTCCACAT TAAGTGATGA AGATGAAGAT AATGGCTCAG ACCTATTTGG TGATTCTGAC
    AAAGAAGAAG AGGAAGAAGA CAATAAAACT ACCAAAACAA GAACAAAATC TTTTGCTGAT
    GAACTGGCTG CTCGAATACA AAGCGATATC CCCAGGACAC AGGAATCCGA TCAATCATCT
    GTTGCATCTC TGGAGGTGAA AAAAAAGAAA GAAAAAGATA AGAAAGAAGT AAAAAAACTT
    CCCTCAGATG ATGAAGATGA TTTTTTCAAA CCACCCAAGC TTACCGATGA AGATTTCTCA
    CCTTTTGGCA GTAAAGGAGG GCTTTTCAGT GGTGGAAAAG GACTTTTTGA TGACGATGAT
    GATGAGGGTG ATATCTTTGC TGATGTCCCC AGCAAGGAAG TCAAAAAAGC AGAACCAAGT
    CCATTGATAA ATTCAGAGAC AGCACATTTG AAAAAGAAGC CACCTGTTGG AGGGGTCTCT
    CTTTTCCCAG GTGGAGAAAA TGTGATTGGG TCCTCAATCC TGGCAGAAAA GGATAAAAAT
    AAATCTACAA CTCCTACACA AGATGTTGCT CCCAAGCCTC CACCAAGCTC AAGTCTATTT
    GACGATGATG ATGGTGCACT GTTTGGGTCA TCCAATTCTA ATCACACTAC AAATAAACCA
    GGAAAAAGTC AACCAACAAC TGATCTGTTT GCAGATGATA ATGATTTGTT TCAAGACAAA
    CCTGTTGCCC CTATTCTAGC AAAGAACAAA GTGACAAAAG AGAATGAACC ACCTAAGGAG
    AACAATATAG AAAGCAAGAA CCATCAAGTG CCTGTTGAAA AATCTAAACC CCTTGAATCT
    TCGACAAAAA AACAAACCAA AGGTCTCTTT TCTGATGAGG ATGATTCCGA GTCGGATCTG
    TTTTCCCCCA TTCCAAGTGC CAGTAAGAGT AAAACAGCCA CCTTACCAGC AGCAAAGACA
    GGCAAAGCCT TGTCCCTGTT TGATGATGAA GATGAAGAGG ATTTGTTCGC TTCTGTACCA
    GATAAGAAGC AGACAGCCCC TGTCACAAAG CCAGCACAGC AAAACAAATT AACTTCTGAA
    GAGAAGGCTC CAAAATCAGG CCTCTTCAGC AGTGATGAAG AGGATCCCAT CGGAACCTTA
    AATCAAAAAA ATACAGTTGA AAAAACTCAG CCTGCAAAAG CTGCAGCAGT TGTATCAAAA
    GGAACGAGGG AAGAGCCATT GGGGAAGAAG TCAAGTTTGT TTGATAATGA CACAGATGAT
    CTTTTTGCAA TCACAAAAGA GAGTGAAAAG AAGCATAATC GTGTTTCACT GTTATTTGAA
    GATGACAATG AAGGATCTTT ATTTTCTTCA GTATCTACAA CAGCTGCTTC TACAAGCCAA
    GCTCCTGTAA ACACCATAGA AGAATCTAAA CTACCAGATA CTCATGCACT CATCTCTAAG
    AAGGAATCTT TGTTAACTAA ACCAACAAAA ACAGAGGGTC AAAAATCAGA GCCTGAACCA
    CCAGTAAGTG TCTGTGAGAA TAAGGAACCA TTGCAGGAAA ATAAAAAGGT GAAACAAGCT
    GTTTTCTCAG AGCCAGGGGA GGATAATCTC TTTGCAGCAT CCCCTCCTGC CGATAAACAT
    GCAAAAATCA AAAGTAAAAA TGTGCTAAGT CTCTTTGATG ATGAAGACGA GGACTTCGAA
    AATCATACAG ATAAAACTAT TACACAAAGA ATACCTGGAA AGGTCTCCTC CGAGACGAGC
    TCCCACAAAA AGAGAACAGG CGTGTTCCAA GATGAAGAAC TTCTGTTCAG CCAAGAATTA
    CAGAAAGACA ATGACCCTGA TGTGGACCTG TTTGCTAGCG CTAAAAAGCC ACCGTCTGAA
    AAACCAAGCC ATACAAAATC ATCAGCTGGA ATGGGCTTGT TTGGGGACGA TGAGGACGAT
    GATCTTTTTA GTACAGCTAA ACCAAAAAAG CCTCCGAAAG TGCCAGAGAA GAAACCAGTG
    AACATAAAAG AAAAATCTGA TGCCATTAAA GAACCCCCCA AAGCAAGTGA AGTGAAAAAA
    GATAATCTTT TGAGGAGAGA AAATGCAGAG GCAAATCTGC AGATTAACCC AGCTGCAATG
    TTACCAGGGG CAGTCCCCAA ATTAGCAGGA GTCAGGCCTG CCATTGTGGG CATTTCACCA
    CCACCCCTTG CAGATACGAT GGACAACAGC ACCTCGCCCA CTGCTAGTAC CGGCATACAA
    GGAGTCAGCT TTGAAAGCCC AGCACAGATT GATACTCTTC ACAGTGCAAA TAAGGCACGA
    CCCAGGAATG CCAGAAGGCG ACCACCTACA AGAATGGGCA GAAAACTTGC ATCACAAGAC
    TCTGGTGAAA CAATTGACGA CTCTGGAGTT TCACCCACCT CGCATACGGC TGCTACCACG
    CCTGAATTGG CACAATTGAA CTCGCACCAG CAATTCGATT CAACTGTTTC TAATATGAAG
    CAGTCACTGT CAGAGAATGA CGTACAGTCC AGAGTGAACA CAAAAGTTAC AACAAAACCT
    CTAACACCCA ACATAAATGA CTTGTTTGGG TCTGATCTTT TTGCAAAACG TTCATTACCC
    CCTGCTGATG CCTCAAAAAA GCAAGATGGT CCTCCTGATA ACTCATCTGC AAACAAACCT
    GGGCTGGGGA CTGGAACTAA ATCTCCTGCT TCAGTGATAG ATGAGCAGAA CAGCGATGAC
    GACCTTTTTC AGACAGTTAA ACAGAAGTCT GTAAAAATGC CCAAAGGGGC CTCATTAGTG
    GATAATGAAC CCAGCGATGA CCTTTTCAGA GTTTCAAAAT CCCAGAAGAA AGTGGATATA
    AAGACTGTCC AGCCTAAGGA AACAAAATCA AAAGCCAGCA ATATATTTGA GGATGACATA
    TTCGCAACTG AAGTAGTAAA ACCAGTAAAG AAAACAAAAG AAAAGAAACC ACCATCAGAA
    TCCAATCTCT TTGACGAGAA CGTGGATATA TTTGCTGATC TAACTCAAAA AGGTAAAGAG
    AAGAAAGCTA AGAAGAAGGT TGAGCCCAAA TCAATATTTG ATGATGATAT GGATGACATA
    TTTGCATCAG GAAGTGTAAA ACATACCAAG CAAAAAGCGA AACCCAGTGA AACTTCTGCG
    GAGGTGAAAT CTGAGAGCAA CGTTTCCAGC ATATTTGATG ACCCTTTAAA TGTTTTCAGC
    AAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031762247.1
    CDS144..4049
    Translation

    Target ORF information:

    RefSeq Version XM_031906387.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis WASH complex subunit 2A (washc2a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031906387.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAACGGGC CGGCGAATGG TTCGGCAGAG ACGCCAGTGT GGGAGAGACC CTGGGGCTTG 
    GAGGAGATTA GGAGGAGCAG CCAGAACTGG TCCCTGGGTG CTGATGCGGG GCTCTTGAAT
    TTCCTCCGGG AGTTCTCTCA GCAAACCATA TCAAGGACAC ATGAAATAGA GAAACAACTG
    GATGGGCTCA TTCGTGAAAC AAAGGCCACA GACTGTCGTC TACACAATGT CTTTAATGAT
    TTTCTTATGT TATCTAACAC TCAGTTCATA GAAAATAGAG TGTATGATGA AGAAGTTGAA
    GATCATGTTG TTAAGGCAGA TGCTGGGAAC AAACCTGAAC AGGAGAAAAC TCGGGAGCAA
    AAGGAAGCAG AGCTGATTCC AAAAATACAG GAAGCTGTAA ACTATGGACT GCAAGTACTG
    GAAAATGCTT TTGAGCAACT GGATATTAAA GCAGGGAATT CTGACTCGGA GGAAGAAGAA
    GTTAATGAGC GTGTGGAGCC TATATTAGAG CCAAAGGATC TTTATATAGA CAGACCTCTG
    CCGCTTTTAA TTGGCTCCCA ACAGTTCATG CAGCAAGAAG ATGTTGGCCT TGGAGATCTC
    TCAAGTGAAG AGGGATCGGT GGACAATGAT CGGGGGAGCA TTATTGACAG TGAAGATGAA
    AAAGATGATG AGGAATCAGA AGATGATTTT GACAACAATT CTGAGGAGGA AAAGAAATTG
    CACTCCACAT TAAGTGATGA AGATGAAGAT AATGGCTCAG ACCTATTTGG TGATTCTGAC
    AAAGAAGAAG AGGAAGAAGA CAATAAAACT ACCAAAACAA GAACAAAATC TTTTGCTGAT
    GAACTGGCTG CTCGAATACA AAGCGATATC CCCAGGACAC AGGAATCCGA TCAATCATCT
    GTTGCATCTC TGGAGGTGAA AAAAAAGAAA GAAAAAGATA AGAAAGAAGT AAAAAAACTT
    CCCTCAGATG ATGAAGATGA TTTTTTCAAA CCACCCAAGC TTACCGATGA AGATTTCTCA
    CCTTTTGGCA GTAAAGGAGG GCTTTTCAGT GGTGGAAAAG GACTTTTTGA TGACGATGAT
    GATGAGGGTG ATATCTTTGC TGATGTCCCC AGCAAGGAAG TCAAAAAAGC AGAACCAAGT
    CCATTGATAA ATTCAGAGAC AGCACATTTG AAAAAGAAGC CACCTGTTGG AGGGGTCTCT
    CTTTTCCCAG GTGGAGAAAA TGTGATTGGG TCCTCAATCC TGGCAGAAAA GGATAAAAAT
    AAATCTACAA CTCCTACACA AGATGTTGCT CCCAAGCCTC CACCAAGCTC AAGTCTATTT
    GACGATGATG ATGGTGCACT GTTTGGGTCA TCCAATTCTA ATCACACTAC AAATAAACCA
    GGAAAAAGTC AACCAACAAC TGATCTGTTT GCAGATGATA ATGATTTGTT TCAAGACAAA
    CCTGTTGCCC CTATTCTAGC AAAGAACAAA GTGACAAAAG AGAATGAACC ACCTAAGGAG
    AACAATATAG AAAGCAAGAA CCATCAAGTG CCTGTTGAAA AATCTAAACC CCTTGAATCT
    TCGACAAAAA AACAAACCAA AGGTCTCTTT TCTGATGAGG ATGATTCCGA GTCGGATCTG
    TTTTCCCCCA TTCCAAGTGC CAGTAAGAGT AAAACAGCCA CCTTACCAGC AGCAAAGACA
    GGCAAAGCCT TGTCCCTGTT TGATGATGAA GATGAAGAGG ATTTGTTCGC TTCTGTACCA
    GATAAGAAGC AGACAGCCCC TGTCACAAAG CCAGCACAGC AAAACAAATT AACTTCTGAA
    GAGAAGGCTC CAAAATCAGG CCTCTTCAGC AGTGATGAAG AGGATCCCAT CGGAACCTTA
    AATCAAAAAA ATACAGTTGA AAAAACTCAG CCTGCAAAAG CTGCAGCAGT TGTATCAAAA
    GGAACGAGGG AAGAGCCATT GGGGAAGAAG TCAAGTTTGT TTGATAATGA CACAGATGAT
    CTTTTTGCAA TCACAAAAGA GAGTGAAAAG AAGCATAATC GTGTTTCACT GTTATTTGAA
    GATGACAATG AAGGATCTTT ATTTTCTTCA GTATCTACAA CAGCTGCTTC TACAAGCCAA
    GCTCCTGTAA ACACCATAGA AGAATCTAAA CTACCAGATA CTCATGCACT CATCTCTAAG
    AAGGAATCTT TGTTAACTAA ACCAACAAAA ACAGAGGGTC AAAAATCAGA GCCTGAACCA
    CCAGTAAGTG TCTGTGAGAA TAAGGAACCA TTGCAGGAAA ATAAAAAGGT GAAACAAGCT
    GTTTTCTCAG AGCCAGGGGA GGATAATCTC TTTGCAGCAT CCCCTCCTGC CGATAAACAT
    GCAAAAATCA AAAGTAAAAA TGTGCTAAGT CTCTTTGATG ATGAAGACGA GGACTTCGAA
    AATCATACAG ATAAAACTAT TACACAAAGA ATACCTGGAA AGGTCTCCTC CGAGACGAGC
    TCCCACAAAA AGAGAACAGG CGTGTTCCAA GATGAAGAAC TTCTGTTCAG CCAAGAATTA
    CAGAAAGACA ATGACCCTGA TGTGGACCTG TTTGCTAGCG CTAAAAAGCC ACCGTCTGAA
    AAACCAAGCC ATACAAAATC ATCAGCTGGA ATGGGCTTGT TTGGGGACGA TGAGGACGAT
    GATCTTTTTA GTACAGCTAA ACCAAAAAAG CCTCCGAAAG TGCCAGAGAA GAAACCAGTG
    AACATAAAAG AAAAATCTGA TGCCATTAAA GAACCCCCCA AAGCAAGTGA AGTGAAAAAA
    GATAATCTTT TGAGGAGAGA AAATGCAGAG GCAAATCTGC AGATTAACCC AGCTGCAATG
    TTACCAGGGG CAGTCCCCAA ATTAGCAGGA GTCAGGCCTG CCATTGTGGG CATTTCACCA
    CCACCCCTTG CAGATACGAT GGACAACAGC ACCTCGCCCA CTGCTAGTAC CGGCATACAA
    GGAGTCAGCT TTGAAAGCCC AGCACAGATT GATACTCTTC ACAGTGCAAA TAAGGCACGA
    CCCAGGAATG CCAGAAGGCG ACCACCTACA AGAATGGGCA GAAAACTTGC ATCACAAGAC
    TCTGGTGAAA CAATTGACGA CTCTGGAGTT TCACCCACCT CGCATACGGC TGCTACCACG
    CCTGAATTGG CACAATTGAA CTCGCACCAG CAATTCGATT CAACTGTTTC TAATATGAAG
    CAGTCACTGT CAGAGAATGA CGTACAGTCC AGAGTGAACA CAAAAGTTAC AACAAAACCT
    CTAACACCCA ACATAAATGA CTTGTTTGGG TCTGATCTTT TTGCAAAACG TTCATTACCC
    CCTGCTGATG CCTCAAAAAA GCAAGATGGT CCTCCTGATA ACTCATCTGC AAACAAACCT
    GGGCTGGGGA CTGGAACTAA ATCTCCTGCT TCAGTGATAG ATGAGCAGAA CAGCGATGAC
    GACCTTTTTC AGACAGTTAA ACAGAAGTCT GTAAAAATGC CCAAAGGGGC CTCATTAGTG
    GATAATGAAC CCAGCGATGA CCTTTTCAGA GTTTCAAAAT CCCAGAAGAA AGTGGATATA
    AAGACTGTCC AGCCTAAGGA AACAAAATCA AAAGCCAGCA ATATATTTGA GGATGACATA
    TTCGCAACTG AAGTAGTAAA ACCAGTAAAG AAAACAAAAG AAAAGAAACC ACCATCAGAA
    TCCAATCTCT TTGACGAGAA CGTGGATATA TTTGCTGATC TAACTCAAAA AGGTAAAGAG
    AAGAAAGCTA AGAAGAAGGT TGAGCCCAAA TCAATATTTG ATGATGATAT GGATGACATA
    TTTGCATCAG GAAGTGTAAA ACATACCAAG CAAAAAGCGA AACCCAGTGA AACTTCTGCG
    GAGGTGAAAT CTGAGAGCAA CGTTTCCAGC ATATTTGATG ACCCTTTAAA TGTTTTCAGC
    AAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.