ubr2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ubr2
Entrez Gene ID	100170193
Full Name	ubiquitin protein ligase E3 component n-recognin 2
Synonyms	ba49a4.1
General protein information	Preferred Names ubiquitin protein ligase E3 component n-recognin 2 Names E3 ubiquitin-protein ligase UBR2
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004914083.3	XP_004914140.1	E3 ubiquitin-protein ligase UBR2 isoform X2
XM_002934690.5	XP_002934736.2	E3 ubiquitin-protein ligase UBR2 isoform X3
XM_002934690.4	XP_002934736.1	E3 ubiquitin-protein ligase UBR2 isoform X1
XM_031902908.1	XP_031758768.1	E3 ubiquitin-protein ligase UBR2 isoform X1
XM_031902909.1	XP_031758769.1	E3 ubiquitin-protein ligase UBR2 isoform X2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ubr2	NP_001171542.1
Gallus gallus (chicken)	UBR2	XP_004935398.1
Drosophila melanogaster (fruit fly)	CG9086	NP_001245723.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000171	XP_310967.5
Caenorhabditis elegans (roundworm)	ubr-1	NP_491231.2
Xenopus tropicalis (tropical clawed frog)	ubr2	XP_002934736.1
	UBR2	XP_001088074.2
Bos taurus (cattle)	UBR2	NP_001179567.1
Danio rerio (zebrafish)	ubr2	XP_005156554.1
Mus musculus (house mouse)	Ubr2	NP_001170845.1
Homo sapiens (human)	UBR2	NP_056070.1
Pan troglodytes (chimpanzee)	UBR2	XP_001134707.1
Canis lupus familiaris (dog)	UBR2	XP_005627416.1

Gene Ontology

The following ubr2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ubr2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa59217	XM_031902908.1 Latest version!	Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa59218	XM_031902909.1 Latest version!	Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa59219	XM_002934690.5 Latest version!	Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa09642	XM_002934690.4 Latest version!	Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa09643	XM_004914083.3 Latest version!	Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ubr2 ( XM_031902908.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758768.1 Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X1, mRNA.

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CloneID	OXa59217
Clone ID Related Accession (Same CDS sequence)	XM_031902908.1
Accession Version	XM_031902908.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5349bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase UBR2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTC CAGAGCAAGA AGCTGAAGTG CCAGGAATAG ACAGCGAGTT GTTGGCCTGC 
TGTGCCGGGG AGGTGGCCTT GAAATGGCTC CAAGCAACTG ATCTACCTAA AGAAGTTTAT 
CAGCACCTTG CCTACTATGT ACCCAAAATT TACTGTCAAG GCTCCAACCC AACTCCAGAA 
AATGAGGACA TGGTGGTCCA GCACGTTCTG TTGGGCCCTA TGGAGTGGTA TCTGTGCGGC 
GAGGACCCTG CAATTGGATT TCCAAAGCTT AAGCAAGCCA ACAAGCCATC TCAGCTGTGT 
GGCCGGGTAT TTAAAGTGGG AGAGCCAACA TATTCATGCA GGGACTGTGC AGTCGACCCA 
ACATGCGTTT TATGCATGGA GTGTTTTTTA GGAAGTGTTC ATAGAGAGCA CCGATATCGG 
ATGACAACGT CAGGGGGTGG AGGATTCTGT GATTGTGGAG ATGCTGAAGC ATGGAAAGGA 
GGGCCATACT GCCAGAAACA TGCACTGAAT ATCTCCGAAA GTGAGACAGA GGACCCACTA 
ATTCATTTGT CAGAAGATAT GATAGCAAGA GCATATAATA TGTTTGCCAT TATGTTTCGC 
TACGCAGTGG ATATTCTGAC CTGGGAGAAG GAGAATGAGC TGCCTGATGG ATTAGAGTGT 
GTGAAGAAAG ATGATACCTA TTACTGCATG CTTTTTAATG ATGAAGTACA CACATATGAG 
CAGGTTATAT ACACTCTTCA GAAAGCTGTC AGCTGCAGCC AGAAGGAAGC CATCAGTTTT 
GCAACAACGG TGGACAGGGA TGGGCGGAGA TCAGTTCGAA ATGGGGACTT TCAGTCTTGC 
GAGCAAGCAA AGTCGGTCAT AGTGAGGAAC ACTAGTCGAC AAACAAAACC TCTTAAAGTG 
CAGGTCATGC ATTCTGCTAT TGTTGCACAC CAGACATTTG GGTTGAAGCT CATCAGCTGG 
CTCGGCCAAG TTATTGGGTC CTCAGGTGGA CTTCGTCGCA TCCTCTGCCA GGTTGGTTTA 
CAGGAAGGTC CAGAGGGGGA AAACTCTTCG CTTGTAGACA GACTGATGTT GTGTGATTCA 
AAACTTTGGA AAGGTGCTAG AAACTGCTAT CACCAACTTT TCATGAGCAG TCTGCTAATG 
GATTTAAAGT ATAAGAAACT TTTTGCCATT CGCTTTGCAA AAAACTATGA GCGATTGCAG 
AATGATTATG TGAAAGACGA CCACGACAGG GAATTCTCTA TCACTGACCT TTCGGTACAG 
ATATTCACAG TGCCTTCACT TGCTCGAATG CTGATCACGG AAGAGAATAT GATGGCAACC 
GTAATAAAGA CATTCATGGA CCACTTGAGA ACACGGGATA TTCAGGGCAG ATTTCAGTTT 
GAACGATATA CTGCTCTACA AGCTTTCAAG TTTAGGAGAG TCCAGAGCCT CATTATGGAT 
CTGAAGTATG TGCTAATAAG CAAGCCATTT GAGTGGACAG ATGGCCTACG TTGCAAATTC 
CTAGAGGGGT TTGAAGCCTT CCTTGAATTG CTAAAATGCA TGCAGGGGAT GGACCCAATT 
ACACGCCAGG TTGGCCAGCA TATTGAGATG GAGCCAGAGT GGGAGGCAGC TTTCACTTTG 
CAGATGAAAC TAACTCACAT CATTTCAATG ATTCAAGACT GGTGTTCCTG TGATGAACAC 
GTCCTAATTG AAGCATACAA GAAGTGCCTT ATTGTCCTGA TGCAGTGCCA CAGTGGTTTT 
ACTGATGGGG AGCAACCTAT TACTCTTAGT ATATCGGGGC ATTCAGTAGA GACTATAAGA 
TACTGTGTTT CTCAGGAGAA AGTCAGCATT CACCTGCCTG TTTCACGTTT GCTTGCAGGG 
CTACATGCCT TGCTGAGTAA AAGCGAAGTA GCTTACAAGT TTCCAGAGCA GCTTCCCTTC 
AGTGAACTGA GCCCACCGAT GCTGATTGAG CATCCTCTCC GCAGTTTGGT GTTATGTGCA 
CAAGTACATG CCGGAATGTG GAGAAGAAAT GGCTTTTCAT TAGTGAATCA GATTTATTAC 
TATCACAATG TAAAATGTAG AAAAGAGATG TTTGACAAAG ATATTGTCAT GCTTCAGGCT 
GGAGCGTCTA TGATGGATCC AAACCACTTT CTAATGATTG TGCTCAGTCG TTTCGAACTG 
TATCAGATTT TTAGCACTCC AGATTACGGC AAGAGATTCC GGAAAGAACA CACAAATAAG 
GATATAATTC AACAGAATAA CACACTCATT GAAGAAATGC TGCATCTAAT TATCATGATA 
GTTGGGGAGA GATTCTGTCC TGGAGTGGGA CAAGTCAGCG GAACAGATGT GATAAAACGT 
GAAATTATCC ATCAGCTGAG CATCAAACCA ATGGCCCACA GTGAACTGGT CAAGGCTTTG 
CCCGAGGATG AAAATAAGGA AACTGGAATG GAGACTGTGA TTGAAACGAT AGCTTCATTT 
AAAAAACCAG GCCTAACAGG ACGTGGACTG TATGAACTGA AGCCTGAATG TACCAAGGAT 
TTCAACCTGT TTTACTTTCA TTTCTCAAGG GCCGAACAGT CCAAGGCAGA GGAGGCCCAG 
CGCAAACTGA AGAGGCAGAT TGGCGAAGAT ACAGCTCTCC CTCCACCGGT GCTGCCTCCG 
TTCTGTCCGC TATTTGCCAG TTTGATTAAC ATTCTTCAAT CAGATGTCAT GCTGTGCATC 
ATGGGAACTG TATTGCAATG GGCAGTAGAG CCAAATGGAT ACGCATGGTC GGAATCCATG 
CTTCAAAGGG TTTTGCATCT GATTGGGATG GCATTGCAAG AAGAGCACCA GCACTTACAG 
AATATCAGTG AGGACAACAT TGTCACCTTT AATTTCACGG AAAAGATCTC AAGGCCTGGG 
GAAGTCCCAA ATGCATCACC AAGCATCTTG GCTATGTTAG AAACATTGCA AAATGCAGCA 
CACTTGGAAG TGCATAAGGA TATGATACGC TGGATATTAA AGATGGTCAG TAACATTAAA 
AAAATCAGAG AAAATCCATC TACAAGCCCA GTCGTTGAAA CAGAGGGCAG TGTTTTGGAG 
GAGAATACTC GTGATAAAGA CAAAGCAGAG AGGAAAAGAA AAGCTGAGAT TGCCAGGCTC 
CGTAGAGAGA AGATTATGGC TCAGATGTCA GAAATGCAGC GACATTTCAT CGATGAAAAT 
AGGGAGCTTT TTCAACAGAC GACTGATGAG TTAAATGCTT CGACTTCCAC TGCATACGAT 
CACAGCTGTC ATGCAATGAT GGACACCAAA CTTGTCGCTT TGGGTCCTCA CCAAACCCGA 
ATCAGTGAAC CAAGGCAGCT GGTTACTTGC ATCCTGTGTC AAGAAGAGCA AGCTTTCACA 
GTGGACAACA AAGCCATGGT TCTTGCAGCT TTTATTCAGA GATCGACGGT ATTCTCCAAG 
AACAGGAGCA GAGTCATTAC AGATCCAGAA AAATATGACC CGCTGTGCAT GCACCCAGAC 
TTGGCTTGTG GCACTCACAC AGGAAGCTGT GGACATATAA TGCACGCTCA CTGCTGGCAG 
AGGTACTTTG ATGCTGTGCA AGCCAAGGAG CAGAGAAGGC AGCAGCGCTT ACGGGTACAT 
ACTAGCTACG ATGTGGAAAA TGGTGAATTT CTCTGCCCCC TCTGTGAGTG TCTAAGTAAC 
ACCGTCATCC CCATGCTTCC ACCACCAAGA AGTTTATACC ACAGGTTGGA TTTCTCTGAT 
CAGCCTGATT TAGCAGAATG GGTTAGCACT ACTTCTCAGC AACTCAAAGC ATTGTGTGCG 
CTGCATGCAG AGAAAAGCAG TTCAGGTGAG TTCAGAAAAG AGGAGCCTAA TACCAATATT 
ACCAAAATAA AGGGATTGAT TTTTGTGAAC AGCTTTTCTT TACTCTCTGC AGCTGGTTCA 
GATTCCGAGA TGCCAGCACA AGTGGATATT CCTGAAGGTT TCCGGCCTGA TTTCCAACCA 
AAAAATCCAT ACTCAGAGAG CATTAGGGAG ATGCTGACAA CATTCGGGAC GGCAACATAT 
AAGGTTGGGC TGAATGTGCA CCCAAACGAG CAGGACCCAA GGGTCCCAAT TATGTGCTGG 
GGAAGCTGTG CGTACACGAT ACAAACAATT GAACGCATTT TGGCAGATGA AGAGAGGCCG 
TTATTTGGGA ACTTGCCATG CAGACAGGAT GACTGCCTAA AATCACTGAG CAGATTTGCT 
GCAGCACATT GGACAGTGTG CCCTCTTCCT GTGATGGAAA AACACTTTAG TCGTCTATTT 
GAAACAATTG TGCCTAATGA GGATGCCGAG AATTCCCCAT GCATACTTGA TATTGATATG 
TTTCATTTAC TGGTCAGCTT GGTGGTCTCA TTCCCAGCTC TACAGTGTCA GGACTTTTCA 
GGGAACAGCC TTGCTACAGG AGATCTCCAT CTCTTCCATC TGGTGACCAT GGCACACATT 
GTCCAGATAG TGCTTTCCTG CTCCACAGAG GAAAATGGCA TGGAGCAGGA ATCCACTGAT 
GGAGAAGAAG AACAGGCAGC AATGTCTTTG TATAGGACCA TCTGTCGGTG CAGTGGAAGC 
ACAATGAAAG AATTATCCTC TGGGTGGCAC CTATGCAGAG CAATCAAAAC TTCAATCCTG 
CCGTACCTGA GGTGTGCTGC GTTATTTTTC CATTATTTAA ATGGTGTTCC GGCACCTTCA 
GAGTTTCTCG TGAGTGGAAT GAGCCAGTTT GATTGTCTCT GTAGTTACCT TTCTCTGCCA 
ACCAACCTGG TCTGCCTGTT TCAAGAGAAT TCTAAAGTAA TGGAAACATT AATAAATAGA 
TGGTGTAAAC ATGCAGAGGT GAAAAGGTTT CTGAATGGGG AATGCACTGC TGTTAGCTAT 
CCAAGGGAAT CAAATAAACT TATCGATCTC CCAGAGGACT ACAGCAGCCT AATTAATCAA 
GCATCTAATT TCACGTGCCC TAAATCTGGT GGAGACAAAA GCAGGGCCCC TACGCTCTGC 
CTGGTGTGTG GAACCATGCT GTGCTCCCAG AGTTACTGCT GCCAGACAGA ACTGGATGGG 
GACGACGTGG GGGCCTGCAC TGCCCATACC TACACATGTG GATCTGGCAT GGGCATTTTC 
CTTCGAGTCA GAGAGTGTCA AGTTCTCTTT TTGGCTGGGA AAACAAAGGG CTGCTTCTAT 
GCTCCTCCTT ACTTAGATGA CTATGGAGAG ACAGATCAAG GCCTCCGACG CGGAAACCCC 
CTGCACCTCT GTAAGGAACG CTACAAAAAG ATCCAGAAGC TGTGGCAGCA GCACAGTATT 
ACAGAGGAAA TTGGCCATGC CCAAGAAGCC AATCAGACTC TAACTGGCAT TGAATGGCAG 
CATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758768.1
CDS	70..5418
Translation	MAAPEQEAEVPGIDSELLACCAGEVALKWLQATDLPKEVYQHLAYYVPKIYCQGSNPTPENEDMVVQHVLLGPMEWYLCGEDPAIGFPKLKQANKPSQLCGRVFKVGEPTYSCRDCAVDPTCVLCMECFLGSVHREHRYRMTTSGGGGFCDCGDAEAWKGGPYCQKHALNISESETEDPLIHLSEDMIARAYNMFAIMFRYAVDILTWEKENELPDGLECVKKDDTYYCMLFNDEVHTYEQVIYTLQKAVSCSQKEAISFATTVDRDGRRSVRNGDFQSCEQAKSVIVRNTSRQTKPLKVQVMHSAIVAHQTFGLKLISWLGQVIGSSGGLRRILCQVGLQEGPEGENSSLVDRLMLCDSKLWKGARNCYHQLFMSSLLMDLKYKKLFAIRFAKNYERLQNDYVKDDHDREFSITDLSVQIFTVPSLARMLITEENMMATVIKTFMDHLRTRDIQGRFQFERYTALQAFKFRRVQSLIMDLKYVLISKPFEWTDGLRCKFLEGFEAFLELLKCMQGMDPITRQVGQHIEMEPEWEAAFTLQMKLTHIISMIQDWCSCDEHVLIEAYKKCLIVLMQCHSGFTDGEQPITLSISGHSVETIRYCVSQEKVSIHLPVSRLLAGLHALLSKSEVAYKFPEQLPFSELSPPMLIEHPLRSLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRKEMFDKDIVMLQAGASMMDPNHFLMIVLSRFELYQIFSTPDYGKRFRKEHTNKDIIQQNNTLIEEMLHLIIMIVGERFCPGVGQVSGTDVIKREIIHQLSIKPMAHSELVKALPEDENKETGMETVIETIASFKKPGLTGRGLYELKPECTKDFNLFYFHFSRAEQSKAEEAQRKLKRQIGEDTALPPPVLPPFCPLFASLINILQSDVMLCIMGTVLQWAVEPNGYAWSESMLQRVLHLIGMALQEEHQHLQNISEDNIVTFNFTEKISRPGEVPNASPSILAMLETLQNAAHLEVHKDMIRWILKMVSNIKKIRENPSTSPVVETEGSVLEENTRDKDKAERKRKAEIARLRREKIMAQMSEMQRHFIDENRELFQQTTDELNASTSTAYDHSCHAMMDTKLVALGPHQTRISEPRQLVTCILCQEEQAFTVDNKAMVLAAFIQRSTVFSKNRSRVITDPEKYDPLCMHPDLACGTHTGSCGHIMHAHCWQRYFDAVQAKEQRRQQRLRVHTSYDVENGEFLCPLCECLSNTVIPMLPPPRSLYHRLDFSDQPDLAEWVSTTSQQLKALCALHAEKSSSGEFRKEEPNTNITKIKGLIFVNSFSLLSAAGSDSEMPAQVDIPEGFRPDFQPKNPYSESIREMLTTFGTATYKVGLNVHPNEQDPRVPIMCWGSCAYTIQTIERILADEERPLFGNLPCRQDDCLKSLSRFAAAHWTVCPLPVMEKHFSRLFETIVPNEDAENSPCILDIDMFHLLVSLVVSFPALQCQDFSGNSLATGDLHLFHLVTMAHIVQIVLSCSTEENGMEQESTDGEEEQAAMSLYRTICRCSGSTMKELSSGWHLCRAIKTSILPYLRCAALFFHYLNGVPAPSEFLVSGMSQFDCLCSYLSLPTNLVCLFQENSKVMETLINRWCKHAEVKRFLNGECTAVSYPRESNKLIDLPEDYSSLINQASNFTCPKSGGDKSRAPTLCLVCGTMLCSQSYCCQTELDGDDVGACTAHTYTCGSGMGIFLRVRECQVLFLAGKTKGCFYAPPYLDDYGETDQGLRRGNPLHLCKERYKKIQKLWQQHSITEEIGHAQEANQTLTGIEWQHL

Target ORF information:

RefSeq Version	XM_031902908.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902908.1

ORF Insert Sequence:

ATGGCGGCTC CAGAGCAAGA AGCTGAAGTG CCAGGAATAG ACAGCGAGTT GTTGGCCTGC 
TGTGCCGGGG AGGTGGCCTT GAAATGGCTC CAAGCAACTG ATCTACCTAA AGAAGTTTAT 
CAGCACCTTG CCTACTATGT ACCCAAAATT TACTGTCAAG GCTCCAACCC AACTCCAGAA 
AATGAGGACA TGGTGGTCCA GCACGTTCTG TTGGGCCCTA TGGAGTGGTA TCTGTGCGGC 
GAGGACCCTG CAATTGGATT TCCAAAGCTT AAGCAAGCCA ACAAGCCATC TCAGCTGTGT 
GGCCGGGTAT TTAAAGTGGG AGAGCCAACA TATTCATGCA GGGACTGTGC AGTCGACCCA 
ACATGCGTTT TATGCATGGA GTGTTTTTTA GGAAGTGTTC ATAGAGAGCA CCGATATCGG 
ATGACAACGT CAGGGGGTGG AGGATTCTGT GATTGTGGAG ATGCTGAAGC ATGGAAAGGA 
GGGCCATACT GCCAGAAACA TGCACTGAAT ATCTCCGAAA GTGAGACAGA GGACCCACTA 
ATTCATTTGT CAGAAGATAT GATAGCAAGA GCATATAATA TGTTTGCCAT TATGTTTCGC 
TACGCAGTGG ATATTCTGAC CTGGGAGAAG GAGAATGAGC TGCCTGATGG ATTAGAGTGT 
GTGAAGAAAG ATGATACCTA TTACTGCATG CTTTTTAATG ATGAAGTACA CACATATGAG 
CAGGTTATAT ACACTCTTCA GAAAGCTGTC AGCTGCAGCC AGAAGGAAGC CATCAGTTTT 
GCAACAACGG TGGACAGGGA TGGGCGGAGA TCAGTTCGAA ATGGGGACTT TCAGTCTTGC 
GAGCAAGCAA AGTCGGTCAT AGTGAGGAAC ACTAGTCGAC AAACAAAACC TCTTAAAGTG 
CAGGTCATGC ATTCTGCTAT TGTTGCACAC CAGACATTTG GGTTGAAGCT CATCAGCTGG 
CTCGGCCAAG TTATTGGGTC CTCAGGTGGA CTTCGTCGCA TCCTCTGCCA GGTTGGTTTA 
CAGGAAGGTC CAGAGGGGGA AAACTCTTCG CTTGTAGACA GACTGATGTT GTGTGATTCA 
AAACTTTGGA AAGGTGCTAG AAACTGCTAT CACCAACTTT TCATGAGCAG TCTGCTAATG 
GATTTAAAGT ATAAGAAACT TTTTGCCATT CGCTTTGCAA AAAACTATGA GCGATTGCAG 
AATGATTATG TGAAAGACGA CCACGACAGG GAATTCTCTA TCACTGACCT TTCGGTACAG 
ATATTCACAG TGCCTTCACT TGCTCGAATG CTGATCACGG AAGAGAATAT GATGGCAACC 
GTAATAAAGA CATTCATGGA CCACTTGAGA ACACGGGATA TTCAGGGCAG ATTTCAGTTT 
GAACGATATA CTGCTCTACA AGCTTTCAAG TTTAGGAGAG TCCAGAGCCT CATTATGGAT 
CTGAAGTATG TGCTAATAAG CAAGCCATTT GAGTGGACAG ATGGCCTACG TTGCAAATTC 
CTAGAGGGGT TTGAAGCCTT CCTTGAATTG CTAAAATGCA TGCAGGGGAT GGACCCAATT 
ACACGCCAGG TTGGCCAGCA TATTGAGATG GAGCCAGAGT GGGAGGCAGC TTTCACTTTG 
CAGATGAAAC TAACTCACAT CATTTCAATG ATTCAAGACT GGTGTTCCTG TGATGAACAC 
GTCCTAATTG AAGCATACAA GAAGTGCCTT ATTGTCCTGA TGCAGTGCCA CAGTGGTTTT 
ACTGATGGGG AGCAACCTAT TACTCTTAGT ATATCGGGGC ATTCAGTAGA GACTATAAGA 
TACTGTGTTT CTCAGGAGAA AGTCAGCATT CACCTGCCTG TTTCACGTTT GCTTGCAGGG 
CTACATGCCT TGCTGAGTAA AAGCGAAGTA GCTTACAAGT TTCCAGAGCA GCTTCCCTTC 
AGTGAACTGA GCCCACCGAT GCTGATTGAG CATCCTCTCC GCAGTTTGGT GTTATGTGCA 
CAAGTACATG CCGGAATGTG GAGAAGAAAT GGCTTTTCAT TAGTGAATCA GATTTATTAC 
TATCACAATG TAAAATGTAG AAAAGAGATG TTTGACAAAG ATATTGTCAT GCTTCAGGCT 
GGAGCGTCTA TGATGGATCC AAACCACTTT CTAATGATTG TGCTCAGTCG TTTCGAACTG 
TATCAGATTT TTAGCACTCC AGATTACGGC AAGAGATTCC GGAAAGAACA CACAAATAAG 
GATATAATTC AACAGAATAA CACACTCATT GAAGAAATGC TGCATCTAAT TATCATGATA 
GTTGGGGAGA GATTCTGTCC TGGAGTGGGA CAAGTCAGCG GAACAGATGT GATAAAACGT 
GAAATTATCC ATCAGCTGAG CATCAAACCA ATGGCCCACA GTGAACTGGT CAAGGCTTTG 
CCCGAGGATG AAAATAAGGA AACTGGAATG GAGACTGTGA TTGAAACGAT AGCTTCATTT 
AAAAAACCAG GCCTAACAGG ACGTGGACTG TATGAACTGA AGCCTGAATG TACCAAGGAT 
TTCAACCTGT TTTACTTTCA TTTCTCAAGG GCCGAACAGT CCAAGGCAGA GGAGGCCCAG 
CGCAAACTGA AGAGGCAGAT TGGCGAAGAT ACAGCTCTCC CTCCACCGGT GCTGCCTCCG 
TTCTGTCCGC TATTTGCCAG TTTGATTAAC ATTCTTCAAT CAGATGTCAT GCTGTGCATC 
ATGGGAACTG TATTGCAATG GGCAGTAGAG CCAAATGGAT ACGCATGGTC GGAATCCATG 
CTTCAAAGGG TTTTGCATCT GATTGGGATG GCATTGCAAG AAGAGCACCA GCACTTACAG 
AATATCAGTG AGGACAACAT TGTCACCTTT AATTTCACGG AAAAGATCTC AAGGCCTGGG 
GAAGTCCCAA ATGCATCACC AAGCATCTTG GCTATGTTAG AAACATTGCA AAATGCAGCA 
CACTTGGAAG TGCATAAGGA TATGATACGC TGGATATTAA AGATGGTCAG TAACATTAAA 
AAAATCAGAG AAAATCCATC TACAAGCCCA GTCGTTGAAA CAGAGGGCAG TGTTTTGGAG 
GAGAATACTC GTGATAAAGA CAAAGCAGAG AGGAAAAGAA AAGCTGAGAT TGCCAGGCTC 
CGTAGAGAGA AGATTATGGC TCAGATGTCA GAAATGCAGC GACATTTCAT CGATGAAAAT 
AGGGAGCTTT TTCAACAGAC GACTGATGAG TTAAATGCTT CGACTTCCAC TGCATACGAT 
CACAGCTGTC ATGCAATGAT GGACACCAAA CTTGTCGCTT TGGGTCCTCA CCAAACCCGA 
ATCAGTGAAC CAAGGCAGCT GGTTACTTGC ATCCTGTGTC AAGAAGAGCA AGCTTTCACA 
GTGGACAACA AAGCCATGGT TCTTGCAGCT TTTATTCAGA GATCGACGGT ATTCTCCAAG 
AACAGGAGCA GAGTCATTAC AGATCCAGAA AAATATGACC CGCTGTGCAT GCACCCAGAC 
TTGGCTTGTG GCACTCACAC AGGAAGCTGT GGACATATAA TGCACGCTCA CTGCTGGCAG 
AGGTACTTTG ATGCTGTGCA AGCCAAGGAG CAGAGAAGGC AGCAGCGCTT ACGGGTACAT 
ACTAGCTACG ATGTGGAAAA TGGTGAATTT CTCTGCCCCC TCTGTGAGTG TCTAAGTAAC 
ACCGTCATCC CCATGCTTCC ACCACCAAGA AGTTTATACC ACAGGTTGGA TTTCTCTGAT 
CAGCCTGATT TAGCAGAATG GGTTAGCACT ACTTCTCAGC AACTCAAAGC ATTGTGTGCG 
CTGCATGCAG AGAAAAGCAG TTCAGGTGAG TTCAGAAAAG AGGAGCCTAA TACCAATATT 
ACCAAAATAA AGGGATTGAT TTTTGTGAAC AGCTTTTCTT TACTCTCTGC AGCTGGTTCA 
GATTCCGAGA TGCCAGCACA AGTGGATATT CCTGAAGGTT TCCGGCCTGA TTTCCAACCA 
AAAAATCCAT ACTCAGAGAG CATTAGGGAG ATGCTGACAA CATTCGGGAC GGCAACATAT 
AAGGTTGGGC TGAATGTGCA CCCAAACGAG CAGGACCCAA GGGTCCCAAT TATGTGCTGG 
GGAAGCTGTG CGTACACGAT ACAAACAATT GAACGCATTT TGGCAGATGA AGAGAGGCCG 
TTATTTGGGA ACTTGCCATG CAGACAGGAT GACTGCCTAA AATCACTGAG CAGATTTGCT 
GCAGCACATT GGACAGTGTG CCCTCTTCCT GTGATGGAAA AACACTTTAG TCGTCTATTT 
GAAACAATTG TGCCTAATGA GGATGCCGAG AATTCCCCAT GCATACTTGA TATTGATATG 
TTTCATTTAC TGGTCAGCTT GGTGGTCTCA TTCCCAGCTC TACAGTGTCA GGACTTTTCA 
GGGAACAGCC TTGCTACAGG AGATCTCCAT CTCTTCCATC TGGTGACCAT GGCACACATT 
GTCCAGATAG TGCTTTCCTG CTCCACAGAG GAAAATGGCA TGGAGCAGGA ATCCACTGAT 
GGAGAAGAAG AACAGGCAGC AATGTCTTTG TATAGGACCA TCTGTCGGTG CAGTGGAAGC 
ACAATGAAAG AATTATCCTC TGGGTGGCAC CTATGCAGAG CAATCAAAAC TTCAATCCTG 
CCGTACCTGA GGTGTGCTGC GTTATTTTTC CATTATTTAA ATGGTGTTCC GGCACCTTCA 
GAGTTTCTCG TGAGTGGAAT GAGCCAGTTT GATTGTCTCT GTAGTTACCT TTCTCTGCCA 
ACCAACCTGG TCTGCCTGTT TCAAGAGAAT TCTAAAGTAA TGGAAACATT AATAAATAGA 
TGGTGTAAAC ATGCAGAGGT GAAAAGGTTT CTGAATGGGG AATGCACTGC TGTTAGCTAT 
CCAAGGGAAT CAAATAAACT TATCGATCTC CCAGAGGACT ACAGCAGCCT AATTAATCAA 
GCATCTAATT TCACGTGCCC TAAATCTGGT GGAGACAAAA GCAGGGCCCC TACGCTCTGC 
CTGGTGTGTG GAACCATGCT GTGCTCCCAG AGTTACTGCT GCCAGACAGA ACTGGATGGG 
GACGACGTGG GGGCCTGCAC TGCCCATACC TACACATGTG GATCTGGCAT GGGCATTTTC 
CTTCGAGTCA GAGAGTGTCA AGTTCTCTTT TTGGCTGGGA AAACAAAGGG CTGCTTCTAT 
GCTCCTCCTT ACTTAGATGA CTATGGAGAG ACAGATCAAG GCCTCCGACG CGGAAACCCC 
CTGCACCTCT GTAAGGAACG CTACAAAAAG ATCCAGAAGC TGTGGCAGCA GCACAGTATT 
ACAGAGGAAA TTGGCCATGC CCAAGAAGCC AATCAGACTC TAACTGGCAT TGAATGGCAG 
CATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ubr2 ( XM_031902909.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758769.1 Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X2, mRNA.

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CloneID	OXa59218
Clone ID Related Accession (Same CDS sequence)	XM_031902909.1
Accession Version	XM_031902909.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5349bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase UBR2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTC CAGAGCAAGA AGCTGAAGTG CCAGGAATAG ACAGCGAGTT GTTGGCCTGC 
TGTGCCGGGG AGGTGGCCTT GAAATGGCTC CAAGCAACTG ATCTACCTAA AGAAGTTTAT 
CAGCACCTTG CCTACTATGT ACCCAAAATT TACTGTCAAG GCTCCAACCC AACTCCAGAA 
AATGAGGACA TGGTGGTCCA GCACGTTCTG TTGGGCCCTA TGGAGTGGTA TCTGTGCGGC 
GAGGACCCTG CAATTGGATT TCCAAAGCTT AAGCAAGCCA ACAAGCCATC TCAGCTGTGT 
GGCCGGGTAT TTAAAGTGGG AGAGCCAACA TATTCATGCA GGGACTGTGC AGTCGACCCA 
ACATGCGTTT TATGCATGGA GTGTTTTTTA GGAAGTGTTC ATAGAGAGCA CCGATATCGG 
ATGACAACGT CAGGGGGTGG AGGATTCTGT GATTGTGGAG ATGCTGAAGC ATGGAAAGGA 
GGGCCATACT GCCAGAAACA TGCACTGAAT ATCTCCGAAA GTGAGACAGA GGACCCACTA 
ATTCATTTGT CAGAAGATAT GATAGCAAGA GCATATAATA TGTTTGCCAT TATGTTTCGC 
TACGCAGTGG ATATTCTGAC CTGGGAGAAG GAGAATGAGC TGCCTGATGG ATTAGAGTGT 
GTGAAGAAAG ATGATACCTA TTACTGCATG CTTTTTAATG ATGAAGTACA CACATATGAG 
CAGGTTATAT ACACTCTTCA GAAAGCTGTC AGCTGCAGCC AGAAGGAAGC CATCAGTTTT 
GCAACAACGG TGGACAGGGA TGGGCGGAGA TCAGTTCGAA ATGGGGACTT TCAGTCTTGC 
GAGCAAGCAA AGTCGGTCAT AGTGAGGAAC ACTAGTCGAC AAACAAAACC TCTTAAAGTG 
CAGGTCATGC ATTCTGCTAT TGTTGCACAC CAGACATTTG GGTTGAAGCT CATCAGCTGG 
CTCGGCCAAG TTATTGGGTC CTCAGGTGGA CTTCGTCGCA TCCTCTGCCA GGTTGGTTTA 
CAGGAAGGTC CAGAGGGGGA AAACTCTTCG CTTGTAGACA GACTGATGTT GTGTGATTCA 
AAACTTTGGA AAGGTGCTAG AAACTGCTAT CACCAACTTT TCATGAGCAG TCTGCTAATG 
GATTTAAAGT ATAAGAAACT TTTTGCCATT CGCTTTGCAA AAAATTACCA GCAGTTGCAG 
AGAGATTTTA TGGAGGATGA TCACGATCGA GCAGTATCGG TGGCTGCTCT GTCTGTTCAG 
CTCTTCACTG TACCCACTCT GGCTCGAATG CTGATCACGG AAGAGAATAT GATGGCAACC 
GTAATAAAGA CATTCATGGA CCACTTGAGA ACACGGGATA TTCAGGGCAG ATTTCAGTTT 
GAACGATATA CTGCTCTACA AGCTTTCAAG TTTAGGAGAG TCCAGAGCCT CATTATGGAT 
CTGAAGTATG TGCTAATAAG CAAGCCATTT GAGTGGACAG ATGGCCTACG TTGCAAATTC 
CTAGAGGGGT TTGAAGCCTT CCTTGAATTG CTAAAATGCA TGCAGGGGAT GGACCCAATT 
ACACGCCAGG TTGGCCAGCA TATTGAGATG GAGCCAGAGT GGGAGGCAGC TTTCACTTTG 
CAGATGAAAC TAACTCACAT CATTTCAATG ATTCAAGACT GGTGTTCCTG TGATGAACAC 
GTCCTAATTG AAGCATACAA GAAGTGCCTT ATTGTCCTGA TGCAGTGCCA CAGTGGTTTT 
ACTGATGGGG AGCAACCTAT TACTCTTAGT ATATCGGGGC ATTCAGTAGA GACTATAAGA 
TACTGTGTTT CTCAGGAGAA AGTCAGCATT CACCTGCCTG TTTCACGTTT GCTTGCAGGG 
CTACATGCCT TGCTGAGTAA AAGCGAAGTA GCTTACAAGT TTCCAGAGCA GCTTCCCTTC 
AGTGAACTGA GCCCACCGAT GCTGATTGAG CATCCTCTCC GCAGTTTGGT GTTATGTGCA 
CAAGTACATG CCGGAATGTG GAGAAGAAAT GGCTTTTCAT TAGTGAATCA GATTTATTAC 
TATCACAATG TAAAATGTAG AAAAGAGATG TTTGACAAAG ATATTGTCAT GCTTCAGGCT 
GGAGCGTCTA TGATGGATCC AAACCACTTT CTAATGATTG TGCTCAGTCG TTTCGAACTG 
TATCAGATTT TTAGCACTCC AGATTACGGC AAGAGATTCC GGAAAGAACA CACAAATAAG 
GATATAATTC AACAGAATAA CACACTCATT GAAGAAATGC TGCATCTAAT TATCATGATA 
GTTGGGGAGA GATTCTGTCC TGGAGTGGGA CAAGTCAGCG GAACAGATGT GATAAAACGT 
GAAATTATCC ATCAGCTGAG CATCAAACCA ATGGCCCACA GTGAACTGGT CAAGGCTTTG 
CCCGAGGATG AAAATAAGGA AACTGGAATG GAGACTGTGA TTGAAACGAT AGCTTCATTT 
AAAAAACCAG GCCTAACAGG ACGTGGACTG TATGAACTGA AGCCTGAATG TACCAAGGAT 
TTCAACCTGT TTTACTTTCA TTTCTCAAGG GCCGAACAGT CCAAGGCAGA GGAGGCCCAG 
CGCAAACTGA AGAGGCAGAT TGGCGAAGAT ACAGCTCTCC CTCCACCGGT GCTGCCTCCG 
TTCTGTCCGC TATTTGCCAG TTTGATTAAC ATTCTTCAAT CAGATGTCAT GCTGTGCATC 
ATGGGAACTG TATTGCAATG GGCAGTAGAG CCAAATGGAT ACGCATGGTC GGAATCCATG 
CTTCAAAGGG TTTTGCATCT GATTGGGATG GCATTGCAAG AAGAGCACCA GCACTTACAG 
AATATCAGTG AGGACAACAT TGTCACCTTT AATTTCACGG AAAAGATCTC AAGGCCTGGG 
GAAGTCCCAA ATGCATCACC AAGCATCTTG GCTATGTTAG AAACATTGCA AAATGCAGCA 
CACTTGGAAG TGCATAAGGA TATGATACGC TGGATATTAA AGATGGTCAG TAACATTAAA 
AAAATCAGAG AAAATCCATC TACAAGCCCA GTCGTTGAAA CAGAGGGCAG TGTTTTGGAG 
GAGAATACTC GTGATAAAGA CAAAGCAGAG AGGAAAAGAA AAGCTGAGAT TGCCAGGCTC 
CGTAGAGAGA AGATTATGGC TCAGATGTCA GAAATGCAGC GACATTTCAT CGATGAAAAT 
AGGGAGCTTT TTCAACAGAC GACTGATGAG TTAAATGCTT CGACTTCCAC TGCATACGAT 
CACAGCTGTC ATGCAATGAT GGACACCAAA CTTGTCGCTT TGGGTCCTCA CCAAACCCGA 
ATCAGTGAAC CAAGGCAGCT GGTTACTTGC ATCCTGTGTC AAGAAGAGCA AGCTTTCACA 
GTGGACAACA AAGCCATGGT TCTTGCAGCT TTTATTCAGA GATCGACGGT ATTCTCCAAG 
AACAGGAGCA GAGTCATTAC AGATCCAGAA AAATATGACC CGCTGTGCAT GCACCCAGAC 
TTGGCTTGTG GCACTCACAC AGGAAGCTGT GGACATATAA TGCACGCTCA CTGCTGGCAG 
AGGTACTTTG ATGCTGTGCA AGCCAAGGAG CAGAGAAGGC AGCAGCGCTT ACGGGTACAT 
ACTAGCTACG ATGTGGAAAA TGGTGAATTT CTCTGCCCCC TCTGTGAGTG TCTAAGTAAC 
ACCGTCATCC CCATGCTTCC ACCACCAAGA AGTTTATACC ACAGGTTGGA TTTCTCTGAT 
CAGCCTGATT TAGCAGAATG GGTTAGCACT ACTTCTCAGC AACTCAAAGC ATTGTGTGCG 
CTGCATGCAG AGAAAAGCAG TTCAGGTGAG TTCAGAAAAG AGGAGCCTAA TACCAATATT 
ACCAAAATAA AGGGATTGAT TTTTGTGAAC AGCTTTTCTT TACTCTCTGC AGCTGGTTCA 
GATTCCGAGA TGCCAGCACA AGTGGATATT CCTGAAGGTT TCCGGCCTGA TTTCCAACCA 
AAAAATCCAT ACTCAGAGAG CATTAGGGAG ATGCTGACAA CATTCGGGAC GGCAACATAT 
AAGGTTGGGC TGAATGTGCA CCCAAACGAG CAGGACCCAA GGGTCCCAAT TATGTGCTGG 
GGAAGCTGTG CGTACACGAT ACAAACAATT GAACGCATTT TGGCAGATGA AGAGAGGCCG 
TTATTTGGGA ACTTGCCATG CAGACAGGAT GACTGCCTAA AATCACTGAG CAGATTTGCT 
GCAGCACATT GGACAGTGTG CCCTCTTCCT GTGATGGAAA AACACTTTAG TCGTCTATTT 
GAAACAATTG TGCCTAATGA GGATGCCGAG AATTCCCCAT GCATACTTGA TATTGATATG 
TTTCATTTAC TGGTCAGCTT GGTGGTCTCA TTCCCAGCTC TACAGTGTCA GGACTTTTCA 
GGGAACAGCC TTGCTACAGG AGATCTCCAT CTCTTCCATC TGGTGACCAT GGCACACATT 
GTCCAGATAG TGCTTTCCTG CTCCACAGAG GAAAATGGCA TGGAGCAGGA ATCCACTGAT 
GGAGAAGAAG AACAGGCAGC AATGTCTTTG TATAGGACCA TCTGTCGGTG CAGTGGAAGC 
ACAATGAAAG AATTATCCTC TGGGTGGCAC CTATGCAGAG CAATCAAAAC TTCAATCCTG 
CCGTACCTGA GGTGTGCTGC GTTATTTTTC CATTATTTAA ATGGTGTTCC GGCACCTTCA 
GAGTTTCTCG TGAGTGGAAT GAGCCAGTTT GATTGTCTCT GTAGTTACCT TTCTCTGCCA 
ACCAACCTGG TCTGCCTGTT TCAAGAGAAT TCTAAAGTAA TGGAAACATT AATAAATAGA 
TGGTGTAAAC ATGCAGAGGT GAAAAGGTTT CTGAATGGGG AATGCACTGC TGTTAGCTAT 
CCAAGGGAAT CAAATAAACT TATCGATCTC CCAGAGGACT ACAGCAGCCT AATTAATCAA 
GCATCTAATT TCACGTGCCC TAAATCTGGT GGAGACAAAA GCAGGGCCCC TACGCTCTGC 
CTGGTGTGTG GAACCATGCT GTGCTCCCAG AGTTACTGCT GCCAGACAGA ACTGGATGGG 
GACGACGTGG GGGCCTGCAC TGCCCATACC TACACATGTG GATCTGGCAT GGGCATTTTC 
CTTCGAGTCA GAGAGTGTCA AGTTCTCTTT TTGGCTGGGA AAACAAAGGG CTGCTTCTAT 
GCTCCTCCTT ACTTAGATGA CTATGGAGAG ACAGATCAAG GCCTCCGACG CGGAAACCCC 
CTGCACCTCT GTAAGGAACG CTACAAAAAG ATCCAGAAGC TGTGGCAGCA GCACAGTATT 
ACAGAGGAAA TTGGCCATGC CCAAGAAGCC AATCAGACTC TAACTGGCAT TGAATGGCAG 
CATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758769.1
CDS	70..5418
Translation	MAAPEQEAEVPGIDSELLACCAGEVALKWLQATDLPKEVYQHLAYYVPKIYCQGSNPTPENEDMVVQHVLLGPMEWYLCGEDPAIGFPKLKQANKPSQLCGRVFKVGEPTYSCRDCAVDPTCVLCMECFLGSVHREHRYRMTTSGGGGFCDCGDAEAWKGGPYCQKHALNISESETEDPLIHLSEDMIARAYNMFAIMFRYAVDILTWEKENELPDGLECVKKDDTYYCMLFNDEVHTYEQVIYTLQKAVSCSQKEAISFATTVDRDGRRSVRNGDFQSCEQAKSVIVRNTSRQTKPLKVQVMHSAIVAHQTFGLKLISWLGQVIGSSGGLRRILCQVGLQEGPEGENSSLVDRLMLCDSKLWKGARNCYHQLFMSSLLMDLKYKKLFAIRFAKNYQQLQRDFMEDDHDRAVSVAALSVQLFTVPTLARMLITEENMMATVIKTFMDHLRTRDIQGRFQFERYTALQAFKFRRVQSLIMDLKYVLISKPFEWTDGLRCKFLEGFEAFLELLKCMQGMDPITRQVGQHIEMEPEWEAAFTLQMKLTHIISMIQDWCSCDEHVLIEAYKKCLIVLMQCHSGFTDGEQPITLSISGHSVETIRYCVSQEKVSIHLPVSRLLAGLHALLSKSEVAYKFPEQLPFSELSPPMLIEHPLRSLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRKEMFDKDIVMLQAGASMMDPNHFLMIVLSRFELYQIFSTPDYGKRFRKEHTNKDIIQQNNTLIEEMLHLIIMIVGERFCPGVGQVSGTDVIKREIIHQLSIKPMAHSELVKALPEDENKETGMETVIETIASFKKPGLTGRGLYELKPECTKDFNLFYFHFSRAEQSKAEEAQRKLKRQIGEDTALPPPVLPPFCPLFASLINILQSDVMLCIMGTVLQWAVEPNGYAWSESMLQRVLHLIGMALQEEHQHLQNISEDNIVTFNFTEKISRPGEVPNASPSILAMLETLQNAAHLEVHKDMIRWILKMVSNIKKIRENPSTSPVVETEGSVLEENTRDKDKAERKRKAEIARLRREKIMAQMSEMQRHFIDENRELFQQTTDELNASTSTAYDHSCHAMMDTKLVALGPHQTRISEPRQLVTCILCQEEQAFTVDNKAMVLAAFIQRSTVFSKNRSRVITDPEKYDPLCMHPDLACGTHTGSCGHIMHAHCWQRYFDAVQAKEQRRQQRLRVHTSYDVENGEFLCPLCECLSNTVIPMLPPPRSLYHRLDFSDQPDLAEWVSTTSQQLKALCALHAEKSSSGEFRKEEPNTNITKIKGLIFVNSFSLLSAAGSDSEMPAQVDIPEGFRPDFQPKNPYSESIREMLTTFGTATYKVGLNVHPNEQDPRVPIMCWGSCAYTIQTIERILADEERPLFGNLPCRQDDCLKSLSRFAAAHWTVCPLPVMEKHFSRLFETIVPNEDAENSPCILDIDMFHLLVSLVVSFPALQCQDFSGNSLATGDLHLFHLVTMAHIVQIVLSCSTEENGMEQESTDGEEEQAAMSLYRTICRCSGSTMKELSSGWHLCRAIKTSILPYLRCAALFFHYLNGVPAPSEFLVSGMSQFDCLCSYLSLPTNLVCLFQENSKVMETLINRWCKHAEVKRFLNGECTAVSYPRESNKLIDLPEDYSSLINQASNFTCPKSGGDKSRAPTLCLVCGTMLCSQSYCCQTELDGDDVGACTAHTYTCGSGMGIFLRVRECQVLFLAGKTKGCFYAPPYLDDYGETDQGLRRGNPLHLCKERYKKIQKLWQQHSITEEIGHAQEANQTLTGIEWQHL

Target ORF information:

RefSeq Version	XM_031902909.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902909.1

ORF Insert Sequence:

ATGGCGGCTC CAGAGCAAGA AGCTGAAGTG CCAGGAATAG ACAGCGAGTT GTTGGCCTGC 
TGTGCCGGGG AGGTGGCCTT GAAATGGCTC CAAGCAACTG ATCTACCTAA AGAAGTTTAT 
CAGCACCTTG CCTACTATGT ACCCAAAATT TACTGTCAAG GCTCCAACCC AACTCCAGAA 
AATGAGGACA TGGTGGTCCA GCACGTTCTG TTGGGCCCTA TGGAGTGGTA TCTGTGCGGC 
GAGGACCCTG CAATTGGATT TCCAAAGCTT AAGCAAGCCA ACAAGCCATC TCAGCTGTGT 
GGCCGGGTAT TTAAAGTGGG AGAGCCAACA TATTCATGCA GGGACTGTGC AGTCGACCCA 
ACATGCGTTT TATGCATGGA GTGTTTTTTA GGAAGTGTTC ATAGAGAGCA CCGATATCGG 
ATGACAACGT CAGGGGGTGG AGGATTCTGT GATTGTGGAG ATGCTGAAGC ATGGAAAGGA 
GGGCCATACT GCCAGAAACA TGCACTGAAT ATCTCCGAAA GTGAGACAGA GGACCCACTA 
ATTCATTTGT CAGAAGATAT GATAGCAAGA GCATATAATA TGTTTGCCAT TATGTTTCGC 
TACGCAGTGG ATATTCTGAC CTGGGAGAAG GAGAATGAGC TGCCTGATGG ATTAGAGTGT 
GTGAAGAAAG ATGATACCTA TTACTGCATG CTTTTTAATG ATGAAGTACA CACATATGAG 
CAGGTTATAT ACACTCTTCA GAAAGCTGTC AGCTGCAGCC AGAAGGAAGC CATCAGTTTT 
GCAACAACGG TGGACAGGGA TGGGCGGAGA TCAGTTCGAA ATGGGGACTT TCAGTCTTGC 
GAGCAAGCAA AGTCGGTCAT AGTGAGGAAC ACTAGTCGAC AAACAAAACC TCTTAAAGTG 
CAGGTCATGC ATTCTGCTAT TGTTGCACAC CAGACATTTG GGTTGAAGCT CATCAGCTGG 
CTCGGCCAAG TTATTGGGTC CTCAGGTGGA CTTCGTCGCA TCCTCTGCCA GGTTGGTTTA 
CAGGAAGGTC CAGAGGGGGA AAACTCTTCG CTTGTAGACA GACTGATGTT GTGTGATTCA 
AAACTTTGGA AAGGTGCTAG AAACTGCTAT CACCAACTTT TCATGAGCAG TCTGCTAATG 
GATTTAAAGT ATAAGAAACT TTTTGCCATT CGCTTTGCAA AAAATTACCA GCAGTTGCAG 
AGAGATTTTA TGGAGGATGA TCACGATCGA GCAGTATCGG TGGCTGCTCT GTCTGTTCAG 
CTCTTCACTG TACCCACTCT GGCTCGAATG CTGATCACGG AAGAGAATAT GATGGCAACC 
GTAATAAAGA CATTCATGGA CCACTTGAGA ACACGGGATA TTCAGGGCAG ATTTCAGTTT 
GAACGATATA CTGCTCTACA AGCTTTCAAG TTTAGGAGAG TCCAGAGCCT CATTATGGAT 
CTGAAGTATG TGCTAATAAG CAAGCCATTT GAGTGGACAG ATGGCCTACG TTGCAAATTC 
CTAGAGGGGT TTGAAGCCTT CCTTGAATTG CTAAAATGCA TGCAGGGGAT GGACCCAATT 
ACACGCCAGG TTGGCCAGCA TATTGAGATG GAGCCAGAGT GGGAGGCAGC TTTCACTTTG 
CAGATGAAAC TAACTCACAT CATTTCAATG ATTCAAGACT GGTGTTCCTG TGATGAACAC 
GTCCTAATTG AAGCATACAA GAAGTGCCTT ATTGTCCTGA TGCAGTGCCA CAGTGGTTTT 
ACTGATGGGG AGCAACCTAT TACTCTTAGT ATATCGGGGC ATTCAGTAGA GACTATAAGA 
TACTGTGTTT CTCAGGAGAA AGTCAGCATT CACCTGCCTG TTTCACGTTT GCTTGCAGGG 
CTACATGCCT TGCTGAGTAA AAGCGAAGTA GCTTACAAGT TTCCAGAGCA GCTTCCCTTC 
AGTGAACTGA GCCCACCGAT GCTGATTGAG CATCCTCTCC GCAGTTTGGT GTTATGTGCA 
CAAGTACATG CCGGAATGTG GAGAAGAAAT GGCTTTTCAT TAGTGAATCA GATTTATTAC 
TATCACAATG TAAAATGTAG AAAAGAGATG TTTGACAAAG ATATTGTCAT GCTTCAGGCT 
GGAGCGTCTA TGATGGATCC AAACCACTTT CTAATGATTG TGCTCAGTCG TTTCGAACTG 
TATCAGATTT TTAGCACTCC AGATTACGGC AAGAGATTCC GGAAAGAACA CACAAATAAG 
GATATAATTC AACAGAATAA CACACTCATT GAAGAAATGC TGCATCTAAT TATCATGATA 
GTTGGGGAGA GATTCTGTCC TGGAGTGGGA CAAGTCAGCG GAACAGATGT GATAAAACGT 
GAAATTATCC ATCAGCTGAG CATCAAACCA ATGGCCCACA GTGAACTGGT CAAGGCTTTG 
CCCGAGGATG AAAATAAGGA AACTGGAATG GAGACTGTGA TTGAAACGAT AGCTTCATTT 
AAAAAACCAG GCCTAACAGG ACGTGGACTG TATGAACTGA AGCCTGAATG TACCAAGGAT 
TTCAACCTGT TTTACTTTCA TTTCTCAAGG GCCGAACAGT CCAAGGCAGA GGAGGCCCAG 
CGCAAACTGA AGAGGCAGAT TGGCGAAGAT ACAGCTCTCC CTCCACCGGT GCTGCCTCCG 
TTCTGTCCGC TATTTGCCAG TTTGATTAAC ATTCTTCAAT CAGATGTCAT GCTGTGCATC 
ATGGGAACTG TATTGCAATG GGCAGTAGAG CCAAATGGAT ACGCATGGTC GGAATCCATG 
CTTCAAAGGG TTTTGCATCT GATTGGGATG GCATTGCAAG AAGAGCACCA GCACTTACAG 
AATATCAGTG AGGACAACAT TGTCACCTTT AATTTCACGG AAAAGATCTC AAGGCCTGGG 
GAAGTCCCAA ATGCATCACC AAGCATCTTG GCTATGTTAG AAACATTGCA AAATGCAGCA 
CACTTGGAAG TGCATAAGGA TATGATACGC TGGATATTAA AGATGGTCAG TAACATTAAA 
AAAATCAGAG AAAATCCATC TACAAGCCCA GTCGTTGAAA CAGAGGGCAG TGTTTTGGAG 
GAGAATACTC GTGATAAAGA CAAAGCAGAG AGGAAAAGAA AAGCTGAGAT TGCCAGGCTC 
CGTAGAGAGA AGATTATGGC TCAGATGTCA GAAATGCAGC GACATTTCAT CGATGAAAAT 
AGGGAGCTTT TTCAACAGAC GACTGATGAG TTAAATGCTT CGACTTCCAC TGCATACGAT 
CACAGCTGTC ATGCAATGAT GGACACCAAA CTTGTCGCTT TGGGTCCTCA CCAAACCCGA 
ATCAGTGAAC CAAGGCAGCT GGTTACTTGC ATCCTGTGTC AAGAAGAGCA AGCTTTCACA 
GTGGACAACA AAGCCATGGT TCTTGCAGCT TTTATTCAGA GATCGACGGT ATTCTCCAAG 
AACAGGAGCA GAGTCATTAC AGATCCAGAA AAATATGACC CGCTGTGCAT GCACCCAGAC 
TTGGCTTGTG GCACTCACAC AGGAAGCTGT GGACATATAA TGCACGCTCA CTGCTGGCAG 
AGGTACTTTG ATGCTGTGCA AGCCAAGGAG CAGAGAAGGC AGCAGCGCTT ACGGGTACAT 
ACTAGCTACG ATGTGGAAAA TGGTGAATTT CTCTGCCCCC TCTGTGAGTG TCTAAGTAAC 
ACCGTCATCC CCATGCTTCC ACCACCAAGA AGTTTATACC ACAGGTTGGA TTTCTCTGAT 
CAGCCTGATT TAGCAGAATG GGTTAGCACT ACTTCTCAGC AACTCAAAGC ATTGTGTGCG 
CTGCATGCAG AGAAAAGCAG TTCAGGTGAG TTCAGAAAAG AGGAGCCTAA TACCAATATT 
ACCAAAATAA AGGGATTGAT TTTTGTGAAC AGCTTTTCTT TACTCTCTGC AGCTGGTTCA 
GATTCCGAGA TGCCAGCACA AGTGGATATT CCTGAAGGTT TCCGGCCTGA TTTCCAACCA 
AAAAATCCAT ACTCAGAGAG CATTAGGGAG ATGCTGACAA CATTCGGGAC GGCAACATAT 
AAGGTTGGGC TGAATGTGCA CCCAAACGAG CAGGACCCAA GGGTCCCAAT TATGTGCTGG 
GGAAGCTGTG CGTACACGAT ACAAACAATT GAACGCATTT TGGCAGATGA AGAGAGGCCG 
TTATTTGGGA ACTTGCCATG CAGACAGGAT GACTGCCTAA AATCACTGAG CAGATTTGCT 
GCAGCACATT GGACAGTGTG CCCTCTTCCT GTGATGGAAA AACACTTTAG TCGTCTATTT 
GAAACAATTG TGCCTAATGA GGATGCCGAG AATTCCCCAT GCATACTTGA TATTGATATG 
TTTCATTTAC TGGTCAGCTT GGTGGTCTCA TTCCCAGCTC TACAGTGTCA GGACTTTTCA 
GGGAACAGCC TTGCTACAGG AGATCTCCAT CTCTTCCATC TGGTGACCAT GGCACACATT 
GTCCAGATAG TGCTTTCCTG CTCCACAGAG GAAAATGGCA TGGAGCAGGA ATCCACTGAT 
GGAGAAGAAG AACAGGCAGC AATGTCTTTG TATAGGACCA TCTGTCGGTG CAGTGGAAGC 
ACAATGAAAG AATTATCCTC TGGGTGGCAC CTATGCAGAG CAATCAAAAC TTCAATCCTG 
CCGTACCTGA GGTGTGCTGC GTTATTTTTC CATTATTTAA ATGGTGTTCC GGCACCTTCA 
GAGTTTCTCG TGAGTGGAAT GAGCCAGTTT GATTGTCTCT GTAGTTACCT TTCTCTGCCA 
ACCAACCTGG TCTGCCTGTT TCAAGAGAAT TCTAAAGTAA TGGAAACATT AATAAATAGA 
TGGTGTAAAC ATGCAGAGGT GAAAAGGTTT CTGAATGGGG AATGCACTGC TGTTAGCTAT 
CCAAGGGAAT CAAATAAACT TATCGATCTC CCAGAGGACT ACAGCAGCCT AATTAATCAA 
GCATCTAATT TCACGTGCCC TAAATCTGGT GGAGACAAAA GCAGGGCCCC TACGCTCTGC 
CTGGTGTGTG GAACCATGCT GTGCTCCCAG AGTTACTGCT GCCAGACAGA ACTGGATGGG 
GACGACGTGG GGGCCTGCAC TGCCCATACC TACACATGTG GATCTGGCAT GGGCATTTTC 
CTTCGAGTCA GAGAGTGTCA AGTTCTCTTT TTGGCTGGGA AAACAAAGGG CTGCTTCTAT 
GCTCCTCCTT ACTTAGATGA CTATGGAGAG ACAGATCAAG GCCTCCGACG CGGAAACCCC 
CTGCACCTCT GTAAGGAACG CTACAAAAAG ATCCAGAAGC TGTGGCAGCA GCACAGTATT 
ACAGAGGAAA TTGGCCATGC CCAAGAAGCC AATCAGACTC TAACTGGCAT TGAATGGCAG 
CATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ubr2 ( XM_002934690.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934736.2 Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa59219
Clone ID Related Accession (Same CDS sequence)	XM_002934690.5
Accession Version	XM_002934690.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase UBR2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002934690.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTC CAGAGCAAGA AGCTGAAGTG CCAGGAATAG ACAGCGAGTT GTTGGCCTGC 
TGTGCCGGGG AGGTGGCCTT GAAATGGCTC CAAGCAACTG ATCTACCTAA AGAAGTTTAT 
CAGCACCTTG CCTACTATGT ACCCAAAATT TACTGTCAAG GCTCCAACCC AACTCCAGAA 
AATGAGGACA TGGTGGTCCA GCACGTTCTG TTGGGCCCTA TGGAGTGGTA TCTGTGCGGC 
GAGGACCCTG CAATTGGATT TCCAAAGCTT AAGCAAGCCA ACAAGCCATC TCAGCTGTGT 
GGCCGGGTAT TTAAAGTGGG AGAGCCAACA TATTCATGCA GGGACTGTGC AGTCGACCCA 
ACATGCGTTT TATGCATGGA GTGTTTTTTA GGAAGTGTTC ATAGAGAGCA CCGATATCGG 
ATGACAACGT CAGGGGGTGG AGGATTCTGT GATTGTGGAG ATGCTGAAGC ATGGAAAGGA 
GGGCCATACT GCCAGAAACA TGCACTGAAT ATCTCCGAAA GTGAGACAGA GGACCCACTA 
ATTCATTTGT CAGAAGATAT GATAGCAAGA GCATATAATA TGTTTGCCAT TATGTTTCGC 
TACGCAGTGG ATATTCTGAC CTGGGAGAAG GAGAATGAGC TGCCTGATGG ATTAGAGTGT 
GTGAAGAAAG ATGATACCTA TTACTGCATG CTTTTTAATG ATGAAGTACA CACATATGAG 
CAGGTTATAT ACACTCTTCA GAAAGCTGTC AGCTGCAGCC AGAAGGAAGC CATCAGTTTT 
GCAACAACGG TGGACAGGGA TGGGCGGAGA TCAGTTCGAA ATGGGGACTT TCAGTCTTGC 
GAGCAAGCAA AGTCGGTCAT AGTGAGGAAC ACTAGTCGAC AAACAAAACC TCTTAAAGTG 
CAGGTCATGC ATTCTGCTAT TGTTGCACAC CAGACATTTG GGTTGAAGCT CATCAGCTGG 
CTCGGCCAAG TTATTGGGTC CTCAGGTGGA CTTCGTCGCA TCCTCTGCCA GGTTGGTTTA 
CAGGAAGGTC CAGAGGGGGA AAACTCTTCG CTTGTAGACA GACTGATGTT GTGTGATTCA 
AAACTTTGGA AAGGTGCTAG AAACTGCTAT CACCAACTTT TCATGAGCAG TCTGCTAATG 
GATTTAAAGT ATAAGAAACT TTTTGCCATT CGCTTTGCAA AAAACTATGA GCGATTGCAG 
AATGATTATG TGAAAGACGA CCACGACAGG GAATTCTCTA TCACTGACCT TTCGGTACAG 
ATATTCACAG TGCCTTCACT TGCTCGAATG CTGATCACGG AAGAGAATAT GATGGCAACC 
GTAATAAAGA CATTCATGGA CCACTTGAGA ACACGGGATA TTCAGGGCAG ATTTCAGTTT 
GAACGATATA CTGCTCTACA AGCTTTCAAG TTTAGGAGAG TCCAGAGCCT CATTATGGAT 
CTGAAGTATG TGCTAATAAG CAAGCCATTT GAGTGGACAG ATGGCCTACG TTGCAAATTC 
CTAGAGGGGT TTGAAGCCTT CCTTGAATTG CTAAAATGCA TGCAGGGGAT GGACCCAATT 
ACACGCCAGG TTGGCCAGCA TATTGAGATG GAGCCAGAGT GGGAGGCAGC TTTCACTTTG 
CAGATGAAAC TAACTCACAT CATTTCAATG ATTCAAGACT GGTGTTCCTG TGATGAACAC 
GTCCTAATTG AAGCATACAA GAAGTGCCTT ATTGTCCTGA TGCAGTGCCA CAGTGGTTTT 
ACTGATGGGG AGCAACCTAT TACTCTTAGT ATATCGGGGC ATTCAGTAGA GACTATAAGA 
TACTGTGTTT CTCAGGAGAA AGTCAGCATT CACCTGCCTG TTTCACGTTT GCTTGCAGGG 
CTACATGCCT TGCTGAGTAA AAGCGAAGTA GCTTACAAGT TTCCAGAGCA GCTTCCCTTC 
AGTGAACTGA GCCCACCGAT GCTGATTGAG CATCCTCTCC GCAGTTTGGT GTTATGTGCA 
CAAGTACATG CCGGAATGTG GAGAAGAAAT GGCTTTTCAT TAGTGAATCA GATTTATTAC 
TATCACAATG TAAAATGTAG AAAAGAGATG TTTGACAAAG ATATTGTCAT GCTTCAGGCT 
GGAGCGTCTA TGATGGATCC AAACCACTTT CTAATGATTG TGCTCAGTCG TTTCGAACTG 
TATCAGATTT TTAGCACTCC AGATTACGGC AAGAGATTCC GGAAAGAACA CACAAATAAG 
GATATAATTC AACAGAATAA CACACTCATT GAAGAAATGC TGCATCTAAT TATCATGATA 
GTTGGGGAGA GATTCTGTCC TGGAGTGGGA CAAGTCAGCG GAACAGATGT GATAAAACGT 
GAAATTATCC ATCAGCTGAG CATCAAACCA ATGGCCCACA GTGAACTGGT CAAGGCTTTG 
CCCGAGGATG AAAATAAGGA AACTGGAATG GAGACTGTGA TTGAAACGAT AGCTTCATTT 
AAAAAACCAG GCCTAACAGG ACGTGGACTG TATGAACTGA AGCCTGAATG TACCAAGGAT 
TTCAACCTGT TTTACTTTCA TTTCTCAAGG GCCGAACAGT CCAAGGCAGA GGAGGCCCAG 
CGCAAACTGA AGAGGCAGAT TGGCGAAGAT ACAGCTCTCC CTCCACCGGT GCTGCCTCCG 
TTCTGTCCGC TATTTGCCAG TTTGATTAAC ATTCTTCAAT CAGATGTCAT GCTGTGCATC 
ATGGGAACTG TATTGCAATG GGCAGTAGAG CCAAATGGAT ACGCATGGTC GGAATCCATG 
CTTCAAAGGG TTTTGCATCT GATTGGGATG GCATTGCAAG AAGAGCACCA GCACTTACAG 
AATATCAGTG AGGACAACAT TGTCACCTTT AATTTCACGG AAAAGATCTC AAGGCCTGGG 
GAAGTCCCAA ATGCATCACC AAGCATCTTG GCTATGTTAG AAACATTGCA AAATGCAGCA 
CACTTGGAAG TGCATAAGGA TATGATACGC TGGATATTAA AGATGGTCAG TAACATTAAA 
AAAATCAGAG AAAATCCATC TACAAGCCCA GTCGTTGAAA CAGAGGGCAG TGTTTTGGAG 
GAGAATACTC GTGATAAAGA CAAAGCAGAG AGGAAAAGAA AAGCTGAGAT TGCCAGGCTC 
CGTAGAGAGA AGATTATGGC TCAGATGTCA GAAATGCAGC GACATTTCAT CGATGAAAAT 
AGGGAGCTTT TTCAACAGAC GACTGATGAG TTAAATGCTT CGACTTCCAC TGCATACGAT 
CACAGCTGTC ATGCAATGAT GGACACCAAA CTTGTCGCTT TGGGTCCTCA CCAAACCCGA 
ATCAGTGAAC CAAGGCAGCT GGTTACTTGC ATCCTGTGTC AAGAAGAGCA AGCTTTCACA 
GTGGACAACA AAGCCATGGT TCTTGCAGCT TTTATTCAGA GATCGACGGT ATTCTCCAAG 
AACAGGAGCA GAGTCATTAC AGATCCAGAA AAATATGACC CGCTGTGCAT GCACCCAGAC 
TTGGCTTGTG GCACTCACAC AGGAAGCTGT GGACATATAA TGCACGCTCA CTGCTGGCAG 
AGGTACTTTG ATGCTGTGCA AGCCAAGGAG CAGAGAAGGC AGCAGCGCTT ACGGGTACAT 
ACTAGCTACG ATGTGGAAAA TGGTGAATTT CTCTGCCCCC TCTGTGAGTG TCTAAGTAAC 
ACCGTCATCC CCATGCTTCC ACCACCAAGA AGTTTATACC ACAGGTTGGA TTTCTCTGAT 
CAGCCTGATT TAGCAGAATG GGTTAGCACT ACTTCTCAGC AACTCAAAGC ATTGTGTGCG 
CTGCATGCAG AGAAAAGCAG TTCAGCTGGT TCAGATTCCG AGATGCCAGC ACAAGTGGAT 
ATTCCTGAAG GTTTCCGGCC TGATTTCCAA CCAAAAAATC CATACTCAGA GAGCATTAGG 
GAGATGCTGA CAACATTCGG GACGGCAACA TATAAGGTTG GGCTGAATGT GCACCCAAAC 
GAGCAGGACC CAAGGGTCCC AATTATGTGC TGGGGAAGCT GTGCGTACAC GATACAAACA 
ATTGAACGCA TTTTGGCAGA TGAAGAGAGG CCGTTATTTG GGAACTTGCC ATGCAGACAG 
GATGACTGCC TAAAATCACT GAGCAGATTT GCTGCAGCAC ATTGGACAGT GTGCCCTCTT 
CCTGTGATGG AAAAACACTT TAGTCGTCTA TTTGAAACAA TTGTGCCTAA TGAGGATGCC 
GAGAATTCCC CATGCATACT TGATATTGAT ATGTTTCATT TACTGGTCAG CTTGGTGGTC 
TCATTCCCAG CTCTACAGTG TCAGGACTTT TCAGGGAACA GCCTTGCTAC AGGAGATCTC 
CATCTCTTCC ATCTGGTGAC CATGGCACAC ATTGTCCAGA TAGTGCTTTC CTGCTCCACA 
GAGGAAAATG GCATGGAGCA GGAATCCACT GATGGAGAAG AAGAACAGGC AGCAATGTCT 
TTGTATAGGA CCATCTGTCG GTGCAGTGGA AGCACAATGA AAGAATTATC CTCTGGGTGG 
CACCTATGCA GAGCAATCAA AACTTCAATC CTGCCGTACC TGAGGTGTGC TGCGTTATTT 
TTCCATTATT TAAATGGTGT TCCGGCACCT TCAGAGTTTC TCGTGAGTGG AATGAGCCAG 
TTTGATTGTC TCTGTAGTTA CCTTTCTCTG CCAACCAACC TGGTCTGCCT GTTTCAAGAG 
AATTCTAAAG TAATGGAAAC ATTAATAAAT AGATGGTGTA AACATGCAGA GGTGAAAAGG 
TTTCTGAATG GGGAATGCAC TGCTGTTAGC TATCCAAGGG AATCAAATAA ACTTATCGAT 
CTCCCAGAGG ACTACAGCAG CCTAATTAAT CAAGCATCTA ATTTCACGTG CCCTAAATCT 
GGTGGAGACA AAAGCAGGGC CCCTACGCTC TGCCTGGTGT GTGGAACCAT GCTGTGCTCC 
CAGAGTTACT GCTGCCAGAC AGAACTGGAT GGGGACGACG TGGGGGCCTG CACTGCCCAT 
ACCTACACAT GTGGATCTGG CATGGGCATT TTCCTTCGAG TCAGAGAGTG TCAAGTTCTC 
TTTTTGGCTG GGAAAACAAA GGGCTGCTTC TATGCTCCTC CTTACTTAGA TGACTATGGA 
GAGACAGATC AAGGCCTCCG ACGCGGAAAC CCCCTGCACC TCTGTAAGGA ACGCTACAAA 
AAGATCCAGA AGCTGTGGCA GCAGCACAGT ATTACAGAGG AAATTGGCCA TGCCCAAGAA 
GCCAATCAGA CTCTAACTGG CATTGAATGG CAGCATTTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934736.2
CDS	69..5330
Translation	MAAPEQEAEVPGIDSELLACCAGEVALKWLQATDLPKEVYQHLAYYVPKIYCQGSNPTPENEDMVVQHVLLGPMEWYLCGEDPAIGFPKLKQANKPSQLCGRVFKVGEPTYSCRDCAVDPTCVLCMECFLGSVHREHRYRMTTSGGGGFCDCGDAEAWKGGPYCQKHALNISESETEDPLIHLSEDMIARAYNMFAIMFRYAVDILTWEKENELPDGLECVKKDDTYYCMLFNDEVHTYEQVIYTLQKAVSCSQKEAISFATTVDRDGRRSVRNGDFQSCEQAKSVIVRNTSRQTKPLKVQVMHSAIVAHQTFGLKLISWLGQVIGSSGGLRRILCQVGLQEGPEGENSSLVDRLMLCDSKLWKGARNCYHQLFMSSLLMDLKYKKLFAIRFAKNYERLQNDYVKDDHDREFSITDLSVQIFTVPSLARMLITEENMMATVIKTFMDHLRTRDIQGRFQFERYTALQAFKFRRVQSLIMDLKYVLISKPFEWTDGLRCKFLEGFEAFLELLKCMQGMDPITRQVGQHIEMEPEWEAAFTLQMKLTHIISMIQDWCSCDEHVLIEAYKKCLIVLMQCHSGFTDGEQPITLSISGHSVETIRYCVSQEKVSIHLPVSRLLAGLHALLSKSEVAYKFPEQLPFSELSPPMLIEHPLRSLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRKEMFDKDIVMLQAGASMMDPNHFLMIVLSRFELYQIFSTPDYGKRFRKEHTNKDIIQQNNTLIEEMLHLIIMIVGERFCPGVGQVSGTDVIKREIIHQLSIKPMAHSELVKALPEDENKETGMETVIETIASFKKPGLTGRGLYELKPECTKDFNLFYFHFSRAEQSKAEEAQRKLKRQIGEDTALPPPVLPPFCPLFASLINILQSDVMLCIMGTVLQWAVEPNGYAWSESMLQRVLHLIGMALQEEHQHLQNISEDNIVTFNFTEKISRPGEVPNASPSILAMLETLQNAAHLEVHKDMIRWILKMVSNIKKIRENPSTSPVVETEGSVLEENTRDKDKAERKRKAEIARLRREKIMAQMSEMQRHFIDENRELFQQTTDELNASTSTAYDHSCHAMMDTKLVALGPHQTRISEPRQLVTCILCQEEQAFTVDNKAMVLAAFIQRSTVFSKNRSRVITDPEKYDPLCMHPDLACGTHTGSCGHIMHAHCWQRYFDAVQAKEQRRQQRLRVHTSYDVENGEFLCPLCECLSNTVIPMLPPPRSLYHRLDFSDQPDLAEWVSTTSQQLKALCALHAEKSSSAGSDSEMPAQVDIPEGFRPDFQPKNPYSESIREMLTTFGTATYKVGLNVHPNEQDPRVPIMCWGSCAYTIQTIERILADEERPLFGNLPCRQDDCLKSLSRFAAAHWTVCPLPVMEKHFSRLFETIVPNEDAENSPCILDIDMFHLLVSLVVSFPALQCQDFSGNSLATGDLHLFHLVTMAHIVQIVLSCSTEENGMEQESTDGEEEQAAMSLYRTICRCSGSTMKELSSGWHLCRAIKTSILPYLRCAALFFHYLNGVPAPSEFLVSGMSQFDCLCSYLSLPTNLVCLFQENSKVMETLINRWCKHAEVKRFLNGECTAVSYPRESNKLIDLPEDYSSLINQASNFTCPKSGGDKSRAPTLCLVCGTMLCSQSYCCQTELDGDDVGACTAHTYTCGSGMGIFLRVRECQVLFLAGKTKGCFYAPPYLDDYGETDQGLRRGNPLHLCKERYKKIQKLWQQHSITEEIGHAQEANQTLTGIEWQHL

Target ORF information:

RefSeq Version	XM_002934690.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934690.5

ORF Insert Sequence:

ATGGCGGCTC CAGAGCAAGA AGCTGAAGTG CCAGGAATAG ACAGCGAGTT GTTGGCCTGC 
TGTGCCGGGG AGGTGGCCTT GAAATGGCTC CAAGCAACTG ATCTACCTAA AGAAGTTTAT 
CAGCACCTTG CCTACTATGT ACCCAAAATT TACTGTCAAG GCTCCAACCC AACTCCAGAA 
AATGAGGACA TGGTGGTCCA GCACGTTCTG TTGGGCCCTA TGGAGTGGTA TCTGTGCGGC 
GAGGACCCTG CAATTGGATT TCCAAAGCTT AAGCAAGCCA ACAAGCCATC TCAGCTGTGT 
GGCCGGGTAT TTAAAGTGGG AGAGCCAACA TATTCATGCA GGGACTGTGC AGTCGACCCA 
ACATGCGTTT TATGCATGGA GTGTTTTTTA GGAAGTGTTC ATAGAGAGCA CCGATATCGG 
ATGACAACGT CAGGGGGTGG AGGATTCTGT GATTGTGGAG ATGCTGAAGC ATGGAAAGGA 
GGGCCATACT GCCAGAAACA TGCACTGAAT ATCTCCGAAA GTGAGACAGA GGACCCACTA 
ATTCATTTGT CAGAAGATAT GATAGCAAGA GCATATAATA TGTTTGCCAT TATGTTTCGC 
TACGCAGTGG ATATTCTGAC CTGGGAGAAG GAGAATGAGC TGCCTGATGG ATTAGAGTGT 
GTGAAGAAAG ATGATACCTA TTACTGCATG CTTTTTAATG ATGAAGTACA CACATATGAG 
CAGGTTATAT ACACTCTTCA GAAAGCTGTC AGCTGCAGCC AGAAGGAAGC CATCAGTTTT 
GCAACAACGG TGGACAGGGA TGGGCGGAGA TCAGTTCGAA ATGGGGACTT TCAGTCTTGC 
GAGCAAGCAA AGTCGGTCAT AGTGAGGAAC ACTAGTCGAC AAACAAAACC TCTTAAAGTG 
CAGGTCATGC ATTCTGCTAT TGTTGCACAC CAGACATTTG GGTTGAAGCT CATCAGCTGG 
CTCGGCCAAG TTATTGGGTC CTCAGGTGGA CTTCGTCGCA TCCTCTGCCA GGTTGGTTTA 
CAGGAAGGTC CAGAGGGGGA AAACTCTTCG CTTGTAGACA GACTGATGTT GTGTGATTCA 
AAACTTTGGA AAGGTGCTAG AAACTGCTAT CACCAACTTT TCATGAGCAG TCTGCTAATG 
GATTTAAAGT ATAAGAAACT TTTTGCCATT CGCTTTGCAA AAAACTATGA GCGATTGCAG 
AATGATTATG TGAAAGACGA CCACGACAGG GAATTCTCTA TCACTGACCT TTCGGTACAG 
ATATTCACAG TGCCTTCACT TGCTCGAATG CTGATCACGG AAGAGAATAT GATGGCAACC 
GTAATAAAGA CATTCATGGA CCACTTGAGA ACACGGGATA TTCAGGGCAG ATTTCAGTTT 
GAACGATATA CTGCTCTACA AGCTTTCAAG TTTAGGAGAG TCCAGAGCCT CATTATGGAT 
CTGAAGTATG TGCTAATAAG CAAGCCATTT GAGTGGACAG ATGGCCTACG TTGCAAATTC 
CTAGAGGGGT TTGAAGCCTT CCTTGAATTG CTAAAATGCA TGCAGGGGAT GGACCCAATT 
ACACGCCAGG TTGGCCAGCA TATTGAGATG GAGCCAGAGT GGGAGGCAGC TTTCACTTTG 
CAGATGAAAC TAACTCACAT CATTTCAATG ATTCAAGACT GGTGTTCCTG TGATGAACAC 
GTCCTAATTG AAGCATACAA GAAGTGCCTT ATTGTCCTGA TGCAGTGCCA CAGTGGTTTT 
ACTGATGGGG AGCAACCTAT TACTCTTAGT ATATCGGGGC ATTCAGTAGA GACTATAAGA 
TACTGTGTTT CTCAGGAGAA AGTCAGCATT CACCTGCCTG TTTCACGTTT GCTTGCAGGG 
CTACATGCCT TGCTGAGTAA AAGCGAAGTA GCTTACAAGT TTCCAGAGCA GCTTCCCTTC 
AGTGAACTGA GCCCACCGAT GCTGATTGAG CATCCTCTCC GCAGTTTGGT GTTATGTGCA 
CAAGTACATG CCGGAATGTG GAGAAGAAAT GGCTTTTCAT TAGTGAATCA GATTTATTAC 
TATCACAATG TAAAATGTAG AAAAGAGATG TTTGACAAAG ATATTGTCAT GCTTCAGGCT 
GGAGCGTCTA TGATGGATCC AAACCACTTT CTAATGATTG TGCTCAGTCG TTTCGAACTG 
TATCAGATTT TTAGCACTCC AGATTACGGC AAGAGATTCC GGAAAGAACA CACAAATAAG 
GATATAATTC AACAGAATAA CACACTCATT GAAGAAATGC TGCATCTAAT TATCATGATA 
GTTGGGGAGA GATTCTGTCC TGGAGTGGGA CAAGTCAGCG GAACAGATGT GATAAAACGT 
GAAATTATCC ATCAGCTGAG CATCAAACCA ATGGCCCACA GTGAACTGGT CAAGGCTTTG 
CCCGAGGATG AAAATAAGGA AACTGGAATG GAGACTGTGA TTGAAACGAT AGCTTCATTT 
AAAAAACCAG GCCTAACAGG ACGTGGACTG TATGAACTGA AGCCTGAATG TACCAAGGAT 
TTCAACCTGT TTTACTTTCA TTTCTCAAGG GCCGAACAGT CCAAGGCAGA GGAGGCCCAG 
CGCAAACTGA AGAGGCAGAT TGGCGAAGAT ACAGCTCTCC CTCCACCGGT GCTGCCTCCG 
TTCTGTCCGC TATTTGCCAG TTTGATTAAC ATTCTTCAAT CAGATGTCAT GCTGTGCATC 
ATGGGAACTG TATTGCAATG GGCAGTAGAG CCAAATGGAT ACGCATGGTC GGAATCCATG 
CTTCAAAGGG TTTTGCATCT GATTGGGATG GCATTGCAAG AAGAGCACCA GCACTTACAG 
AATATCAGTG AGGACAACAT TGTCACCTTT AATTTCACGG AAAAGATCTC AAGGCCTGGG 
GAAGTCCCAA ATGCATCACC AAGCATCTTG GCTATGTTAG AAACATTGCA AAATGCAGCA 
CACTTGGAAG TGCATAAGGA TATGATACGC TGGATATTAA AGATGGTCAG TAACATTAAA 
AAAATCAGAG AAAATCCATC TACAAGCCCA GTCGTTGAAA CAGAGGGCAG TGTTTTGGAG 
GAGAATACTC GTGATAAAGA CAAAGCAGAG AGGAAAAGAA AAGCTGAGAT TGCCAGGCTC 
CGTAGAGAGA AGATTATGGC TCAGATGTCA GAAATGCAGC GACATTTCAT CGATGAAAAT 
AGGGAGCTTT TTCAACAGAC GACTGATGAG TTAAATGCTT CGACTTCCAC TGCATACGAT 
CACAGCTGTC ATGCAATGAT GGACACCAAA CTTGTCGCTT TGGGTCCTCA CCAAACCCGA 
ATCAGTGAAC CAAGGCAGCT GGTTACTTGC ATCCTGTGTC AAGAAGAGCA AGCTTTCACA 
GTGGACAACA AAGCCATGGT TCTTGCAGCT TTTATTCAGA GATCGACGGT ATTCTCCAAG 
AACAGGAGCA GAGTCATTAC AGATCCAGAA AAATATGACC CGCTGTGCAT GCACCCAGAC 
TTGGCTTGTG GCACTCACAC AGGAAGCTGT GGACATATAA TGCACGCTCA CTGCTGGCAG 
AGGTACTTTG ATGCTGTGCA AGCCAAGGAG CAGAGAAGGC AGCAGCGCTT ACGGGTACAT 
ACTAGCTACG ATGTGGAAAA TGGTGAATTT CTCTGCCCCC TCTGTGAGTG TCTAAGTAAC 
ACCGTCATCC CCATGCTTCC ACCACCAAGA AGTTTATACC ACAGGTTGGA TTTCTCTGAT 
CAGCCTGATT TAGCAGAATG GGTTAGCACT ACTTCTCAGC AACTCAAAGC ATTGTGTGCG 
CTGCATGCAG AGAAAAGCAG TTCAGCTGGT TCAGATTCCG AGATGCCAGC ACAAGTGGAT 
ATTCCTGAAG GTTTCCGGCC TGATTTCCAA CCAAAAAATC CATACTCAGA GAGCATTAGG 
GAGATGCTGA CAACATTCGG GACGGCAACA TATAAGGTTG GGCTGAATGT GCACCCAAAC 
GAGCAGGACC CAAGGGTCCC AATTATGTGC TGGGGAAGCT GTGCGTACAC GATACAAACA 
ATTGAACGCA TTTTGGCAGA TGAAGAGAGG CCGTTATTTG GGAACTTGCC ATGCAGACAG 
GATGACTGCC TAAAATCACT GAGCAGATTT GCTGCAGCAC ATTGGACAGT GTGCCCTCTT 
CCTGTGATGG AAAAACACTT TAGTCGTCTA TTTGAAACAA TTGTGCCTAA TGAGGATGCC 
GAGAATTCCC CATGCATACT TGATATTGAT ATGTTTCATT TACTGGTCAG CTTGGTGGTC 
TCATTCCCAG CTCTACAGTG TCAGGACTTT TCAGGGAACA GCCTTGCTAC AGGAGATCTC 
CATCTCTTCC ATCTGGTGAC CATGGCACAC ATTGTCCAGA TAGTGCTTTC CTGCTCCACA 
GAGGAAAATG GCATGGAGCA GGAATCCACT GATGGAGAAG AAGAACAGGC AGCAATGTCT 
TTGTATAGGA CCATCTGTCG GTGCAGTGGA AGCACAATGA AAGAATTATC CTCTGGGTGG 
CACCTATGCA GAGCAATCAA AACTTCAATC CTGCCGTACC TGAGGTGTGC TGCGTTATTT 
TTCCATTATT TAAATGGTGT TCCGGCACCT TCAGAGTTTC TCGTGAGTGG AATGAGCCAG 
TTTGATTGTC TCTGTAGTTA CCTTTCTCTG CCAACCAACC TGGTCTGCCT GTTTCAAGAG 
AATTCTAAAG TAATGGAAAC ATTAATAAAT AGATGGTGTA AACATGCAGA GGTGAAAAGG 
TTTCTGAATG GGGAATGCAC TGCTGTTAGC TATCCAAGGG AATCAAATAA ACTTATCGAT 
CTCCCAGAGG ACTACAGCAG CCTAATTAAT CAAGCATCTA ATTTCACGTG CCCTAAATCT 
GGTGGAGACA AAAGCAGGGC CCCTACGCTC TGCCTGGTGT GTGGAACCAT GCTGTGCTCC 
CAGAGTTACT GCTGCCAGAC AGAACTGGAT GGGGACGACG TGGGGGCCTG CACTGCCCAT 
ACCTACACAT GTGGATCTGG CATGGGCATT TTCCTTCGAG TCAGAGAGTG TCAAGTTCTC 
TTTTTGGCTG GGAAAACAAA GGGCTGCTTC TATGCTCCTC CTTACTTAGA TGACTATGGA 
GAGACAGATC AAGGCCTCCG ACGCGGAAAC CCCCTGCACC TCTGTAAGGA ACGCTACAAA 
AAGATCCAGA AGCTGTGGCA GCAGCACAGT ATTACAGAGG AAATTGGCCA TGCCCAAGAA 
GCCAATCAGA CTCTAACTGG CATTGAATGG CAGCATTTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ubr2 ( XM_002934690.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934736.1 Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X1, mRNA.

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CloneID	OXa09642
Clone ID Related Accession (Same CDS sequence)	XM_002934690.4
Accession Version	XM_002934690.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase UBR2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002934690.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTC CAGAGCAAGA AGCTGAAGTG CCAGGAATAG ACAGCGAGTT GTTGGCCTGC 
TGTGCCGGGG AGGTGGCCTT GAAATGGCTC CAAGCAACTG ATCTACCTAA AGAAGTTTAT 
CAGCACCTTG CCTACTATGT ACCCAAAATT TACTGTCAAG GCTCCAACCC AACTCCAGAA 
AATGAGGACA TGGTGGTCCA GCACGTTCTG TTGGGCCCTA TGGAGTGGTA TCTGTGCGGC 
GAGGACCCTG CAATTGGATT TCCAAAGCTT AAGCAAGCCA ACAAGCCATC TCAGCTGTGT 
GGCCGGGTAT TTAAAGTGGG AGAGCCAACA TATTCATGCA GGGACTGTGC AGTCGACCCA 
ACATGCGTTT TATGCATGGA GTGTTTTTTA GGAAGTGTTC ATAGAGAGCA CCGATATCGG 
ATGACAACGT CAGGGGGTGG AGGATTCTGT GATTGTGGAG ATGCTGAAGC ATGGAAAGGA 
GGGCCATACT GCCAGAAACA TGCACTGAAT ATCTCCGAAA GTGAGACAGA GGACCCACTA 
ATTCATTTGT CAGAAGATAT GATAGCAAGA GCATATAATA TGTTTGCCAT TATGTTTCGC 
TACGCAGTGG ATATTCTGAC CTGGGAGAAG GAGAATGAGC TGCCTGATGG ATTAGAGTGT 
GTGAAGAAAG ATGATACCTA TTACTGCATG CTTTTTAATG ATGAAGTACA CACATATGAG 
CAGGTTATAT ACACTCTTCA GAAAGCTGTC AGCTGCAGCC AGAAGGAAGC CATCAGTTTT 
GCAACAACGG TGGACAGGGA TGGGCGGAGA TCAGTTCGAA ATGGGGACTT TCAGTCTTGC 
GAGCAAGCAA AGTCGGTCAT AGTGAGGAAC ACTAGTCGAC AAACAAAACC TCTTAAAGTG 
CAGGTCATGC ATTCTGCTAT TGTTGCACAC CAGACATTTG GGTTGAAGCT CATCAGCTGG 
CTCGGCCAAG TTATTGGGTC CTCAGGTGGA CTTCGTCGCA TCCTCTGCCA GGTTGGTTTA 
CAGGAAGGTC CAGAGGGGGA AAACTCTTCG CTTGTAGACA GACTGATGTT GTGTGATTCA 
AAACTTTGGA AAGGTGCTAG AAACTGCTAT CACCAACTTT TCATGAGCAG TCTGCTAATG 
GATTTAAAGT ATAAGAAACT TTTTGCCATT CGCTTTGCAA AAAACTATGA GCGATTGCAG 
AATGATTATG TGAAAGACGA CCACGACAGG GAATTCTCTA TCACTGACCT TTCGGTACAG 
ATATTCACAG TGCCTTCACT TGCTCGAATG CTGATCACGG AAGAGAATAT GATGGCAACC 
GTAATAAAGA CATTCATGGA CCACTTGAGA ACACGGGATA TTCAGGGCAG ATTTCAGTTT 
GAACGATATA CTGCTCTACA AGCTTTCAAG TTTAGGAGAG TCCAGAGCCT CATTATGGAT 
CTGAAGTATG TGCTAATAAG CAAGCCATTT GAGTGGACAG ATGGCCTACG TTGCAAATTC 
CTAGAGGGGT TTGAAGCCTT CCTTGAATTG CTAAAATGCA TGCAGGGGAT GGACCCAATT 
ACACGCCAGG TTGGCCAGCA TATTGAGATG GAGCCAGAGT GGGAGGCAGC TTTCACTTTG 
CAGATGAAAC TAACTCACAT CATTTCAATG ATTCAAGACT GGTGTTCCTG TGATGAACAC 
GTCCTAATTG AAGCATACAA GAAGTGCCTT ATTGTCCTGA TGCAGTGCCA CAGTGGTTTT 
ACTGATGGGG AGCAACCTAT TACTCTTAGT ATATCGGGGC ATTCAGTAGA GACTATAAGA 
TACTGTGTTT CTCAGGAGAA AGTCAGCATT CACCTGCCTG TTTCACGTTT GCTTGCAGGG 
CTACATGCCT TGCTGAGTAA AAGCGAAGTA GCTTACAAGT TTCCAGAGCA GCTTCCCTTC 
AGTGAACTGA GCCCACCGAT GCTGATTGAG CATCCTCTCC GCAGTTTGGT GTTATGTGCA 
CAAGTACATG CCGGAATGTG GAGAAGAAAT GGCTTTTCAT TAGTGAATCA GATTTATTAC 
TATCACAATG TAAAATGTAG AAAAGAGATG TTTGACAAAG ATATTGTCAT GCTTCAGGCT 
GGAGCGTCTA TGATGGATCC AAACCACTTT CTAATGATTG TGCTCAGTCG TTTCGAACTG 
TATCAGATTT TTAGCACTCC AGATTACGGC AAGAGATTCC GGAAAGAACA CACAAATAAG 
GATATAATTC AACAGAATAA CACACTCATT GAAGAAATGC TGCATCTAAT TATCATGATA 
GTTGGGGAGA GATTCTGTCC TGGAGTGGGA CAAGTCAGCG GAACAGATGT GATAAAACGT 
GAAATTATCC ATCAGCTGAG CATCAAACCA ATGGCCCACA GTGAACTGGT CAAGGCTTTG 
CCCGAGGATG AAAATAAGGA AACTGGAATG GAGACTGTGA TTGAAACGAT AGCTTCATTT 
AAAAAACCAG GCCTAACAGG ACGTGGACTG TATGAACTGA AGCCTGAATG TACCAAGGAT 
TTCAACCTGT TTTACTTTCA TTTCTCAAGG GCCGAACAGT CCAAGGCAGA GGAGGCCCAG 
CGCAAACTGA AGAGGCAGAT TGGCGAAGAT ACAGCTCTCC CTCCACCGGT GCTGCCTCCG 
TTCTGTCCGC TATTTGCCAG TTTGATTAAC ATTCTTCAAT CAGATGTCAT GCTGTGCATC 
ATGGGAACTG TATTGCAATG GGCAGTAGAG CCAAATGGAT ACGCATGGTC GGAATCCATG 
CTTCAAAGGG TTTTGCATCT GATTGGGATG GCATTGCAAG AAGAGCACCA GCACTTACAG 
AATATCAGTG AGGACAACAT TGTCACCTTT AATTTCACGG AAAAGATCTC AAGGCCTGGG 
GAAGTCCCAA ATGCATCACC AAGCATCTTG GCTATGTTAG AAACATTGCA AAATGCAGCA 
CACTTGGAAG TGCATAAGGA TATGATACGC TGGATATTAA AGATGGTCAG TAACATTAAA 
AAAATCAGAG AAAATCCATC TACAAGCCCA GTCGTTGAAA CAGAGGGCAG TGTTTTGGAG 
GAGAATACTC GTGATAAAGA CAAAGCAGAG AGGAAAAGAA AAGCTGAGAT TGCCAGGCTC 
CGTAGAGAGA AGATTATGGC TCAGATGTCA GAAATGCAGC GACATTTCAT CGATGAAAAT 
AGGGAGCTTT TTCAACAGAC GACTGATGAG TTAAATGCTT CGACTTCCAC TGCATACGAT 
CACAGCTGTC ATGCAATGAT GGACACCAAA CTTGTCGCTT TGGGTCCTCA CCAAACCCGA 
ATCAGTGAAC CAAGGCAGCT GGTTACTTGC ATCCTGTGTC AAGAAGAGCA AGCTTTCACA 
GTGGACAACA AAGCCATGGT TCTTGCAGCT TTTATTCAGA GATCGACGGT ATTCTCCAAG 
AACAGGAGCA GAGTCATTAC AGATCCAGAA AAATATGACC CGCTGTGCAT GCACCCAGAC 
TTGGCTTGTG GCACTCACAC AGGAAGCTGT GGACATATAA TGCACGCTCA CTGCTGGCAG 
AGGTACTTTG ATGCTGTGCA AGCCAAGGAG CAGAGAAGGC AGCAGCGCTT ACGGGTACAT 
ACTAGCTACG ATGTGGAAAA TGGTGAATTT CTCTGCCCCC TCTGTGAGTG TCTAAGTAAC 
ACCGTCATCC CCATGCTTCC ACCACCAAGA AGTTTATACC ACAGGTTGGA TTTCTCTGAT 
CAGCCTGATT TAGCAGAATG GGTTAGCACT ACTTCTCAGC AACTCAAAGC ATTGTGTGCG 
CTGCATGCAG AGAAAAGCAG TTCAGCTGGT TCAGATTCCG AGATGCCAGC ACAAGTGGAT 
ATTCCTGAAG GTTTCCGGCC TGATTTCCAA CCAAAAAATC CATACTCAGA GAGCATTAGG 
GAGATGCTGA CAACATTCGG GACGGCAACA TATAAGGTTG GGCTGAATGT GCACCCAAAC 
GAGCAGGACC CAAGGGTCCC AATTATGTGC TGGGGAAGCT GTGCGTACAC GATACAAACA 
ATTGAACGCA TTTTGGCAGA TGAAGAGAGG CCGTTATTTG GGAACTTGCC ATGCAGACAG 
GATGACTGCC TAAAATCACT GAGCAGATTT GCTGCAGCAC ATTGGACAGT GTGCCCTCTT 
CCTGTGATGG AAAAACACTT TAGTCGTCTA TTTGAAACAA TTGTGCCTAA TGAGGATGCC 
GAGAATTCCC CATGCATACT TGATATTGAT ATGTTTCATT TACTGGTCAG CTTGGTGGTC 
TCATTCCCAG CTCTACAGTG TCAGGACTTT TCAGGGAACA GCCTTGCTAC AGGAGATCTC 
CATCTTTTCC ATCTGGTGAC CATGGCACAC ATTGTCCAGA TAGTGCTTTC CTGCTCCACA 
GAGGAAAATG GCATGGAGCA GGAATCCACT GATGGAGAAG AAGAACAGGC AGCAATGTCT 
TTGTATAGGA CCATCTGTCG GTGCAGTGGA AGCACAATGA AAGAATTATC CTCTGGGTGG 
CACCTATGCA GAGCAATCAA AACTTCAATC CTGCCGTACC TGAGGTGTGC TGCGTTATTT 
TTCCATTATT TAAATGGTGT TCCAGCACCT TCAGAGTTTC TCGTGAGTGG AATGAGCCAG 
TTTGATTGTC TCTGTAGTTA CCTTTCTTTG CCAACCAACT TGGTCTGCCT GTTTCAAGAG 
AATTCTAAAG TAATGGAAAC ATTAATAAAC AGATGGTGTA AACATGCAGA GGTGAAAAGG 
TTTCTGAATG GGGAACGCAC TGCTGTTAGC TATCCAAGGG AATCAAACAA ACTTATCGAT 
CTTCCAGAGG ACTACAGCAG CCTAATTAAT CAAGCATCTA ATTTCACGTG CCCTAAATCT 
GGTGGAGACA AAAGCAGGGC CCCTACGCTC TGCCTGGTGT GTGGAACCAT GCTGTGCTCC 
CAGAGTTACT GCTGCCAGAC AGAACTGGAT GGGGACGACG TGGGGGCCTG CACTGCCCAT 
ACCTACACGT GTGGATCTGG CATGGGCATT TTCCTTCGAG TCAGAGAGTG TCAAGTTCTC 
TTTTTGGCTG GGAAAACAAA GGGCTGCTTC TATGCTCCTC CTTACTTAGA TGACTATGGA 
GAGACAGATC AAGGCCTCCG ACGCGGAAAC CCCCTGCACC TCTGTAAGGA ACGCTACAAA 
AAGATCCAGA AGCTGTGGCA GCAGCACAGT ATTACAGAGG AAATTGGCCA TGCCCAAGAA 
GCCAATCAGA CTCTAACTGG CATTGAATGG CAGCATTTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934736.1
CDS	74..5335
Translation	MAAPEQEAEVPGIDSELLACCAGEVALKWLQATDLPKEVYQHLAYYVPKIYCQGSNPTPENEDMVVQHVLLGPMEWYLCGEDPAIGFPKLKQANKPSQLCGRVFKVGEPTYSCRDCAVDPTCVLCMECFLGSVHREHRYRMTTSGGGGFCDCGDAEAWKGGPYCQKHALNISESETEDPLIHLSEDMIARAYNMFAIMFRYAVDILTWEKENELPDGLECVKKDDTYYCMLFNDEVHTYEQVIYTLQKAVSCSQKEAISFATTVDRDGRRSVRNGDFQSCEQAKSVIVRNTSRQTKPLKVQVMHSAIVAHQTFGLKLISWLGQVIGSSGGLRRILCQVGLQEGPEGENSSLVDRLMLCDSKLWKGARNCYHQLFMSSLLMDLKYKKLFAIRFAKNYERLQNDYVKDDHDREFSITDLSVQIFTVPSLARMLITEENMMATVIKTFMDHLRTRDIQGRFQFERYTALQAFKFRRVQSLIMDLKYVLISKPFEWTDGLRCKFLEGFEAFLELLKCMQGMDPITRQVGQHIEMEPEWEAAFTLQMKLTHIISMIQDWCSCDEHVLIEAYKKCLIVLMQCHSGFTDGEQPITLSISGHSVETIRYCVSQEKVSIHLPVSRLLAGLHALLSKSEVAYKFPEQLPFSELSPPMLIEHPLRSLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRKEMFDKDIVMLQAGASMMDPNHFLMIVLSRFELYQIFSTPDYGKRFRKEHTNKDIIQQNNTLIEEMLHLIIMIVGERFCPGVGQVSGTDVIKREIIHQLSIKPMAHSELVKALPEDENKETGMETVIETIASFKKPGLTGRGLYELKPECTKDFNLFYFHFSRAEQSKAEEAQRKLKRQIGEDTALPPPVLPPFCPLFASLINILQSDVMLCIMGTVLQWAVEPNGYAWSESMLQRVLHLIGMALQEEHQHLQNISEDNIVTFNFTEKISRPGEVPNASPSILAMLETLQNAAHLEVHKDMIRWILKMVSNIKKIRENPSTSPVVETEGSVLEENTRDKDKAERKRKAEIARLRREKIMAQMSEMQRHFIDENRELFQQTTDELNASTSTAYDHSCHAMMDTKLVALGPHQTRISEPRQLVTCILCQEEQAFTVDNKAMVLAAFIQRSTVFSKNRSRVITDPEKYDPLCMHPDLACGTHTGSCGHIMHAHCWQRYFDAVQAKEQRRQQRLRVHTSYDVENGEFLCPLCECLSNTVIPMLPPPRSLYHRLDFSDQPDLAEWVSTTSQQLKALCALHAEKSSSAGSDSEMPAQVDIPEGFRPDFQPKNPYSESIREMLTTFGTATYKVGLNVHPNEQDPRVPIMCWGSCAYTIQTIERILADEERPLFGNLPCRQDDCLKSLSRFAAAHWTVCPLPVMEKHFSRLFETIVPNEDAENSPCILDIDMFHLLVSLVVSFPALQCQDFSGNSLATGDLHLFHLVTMAHIVQIVLSCSTEENGMEQESTDGEEEQAAMSLYRTICRCSGSTMKELSSGWHLCRAIKTSILPYLRCAALFFHYLNGVPAPSEFLVSGMSQFDCLCSYLSLPTNLVCLFQENSKVMETLINRWCKHAEVKRFLNGERTAVSYPRESNKLIDLPEDYSSLINQASNFTCPKSGGDKSRAPTLCLVCGTMLCSQSYCCQTELDGDDVGACTAHTYTCGSGMGIFLRVRECQVLFLAGKTKGCFYAPPYLDDYGETDQGLRRGNPLHLCKERYKKIQKLWQQHSITEEIGHAQEANQTLTGIEWQHL

Target ORF information:

RefSeq Version	XM_002934690.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934690.4

ORF Insert Sequence:

ATGGCGGCTC CAGAGCAAGA AGCTGAAGTG CCAGGAATAG ACAGCGAGTT GTTGGCCTGC 
TGTGCCGGGG AGGTGGCCTT GAAATGGCTC CAAGCAACTG ATCTACCTAA AGAAGTTTAT 
CAGCACCTTG CCTACTATGT ACCCAAAATT TACTGTCAAG GCTCCAACCC AACTCCAGAA 
AATGAGGACA TGGTGGTCCA GCACGTTCTG TTGGGCCCTA TGGAGTGGTA TCTGTGCGGC 
GAGGACCCTG CAATTGGATT TCCAAAGCTT AAGCAAGCCA ACAAGCCATC TCAGCTGTGT 
GGCCGGGTAT TTAAAGTGGG AGAGCCAACA TATTCATGCA GGGACTGTGC AGTCGACCCA 
ACATGCGTTT TATGCATGGA GTGTTTTTTA GGAAGTGTTC ATAGAGAGCA CCGATATCGG 
ATGACAACGT CAGGGGGTGG AGGATTCTGT GATTGTGGAG ATGCTGAAGC ATGGAAAGGA 
GGGCCATACT GCCAGAAACA TGCACTGAAT ATCTCCGAAA GTGAGACAGA GGACCCACTA 
ATTCATTTGT CAGAAGATAT GATAGCAAGA GCATATAATA TGTTTGCCAT TATGTTTCGC 
TACGCAGTGG ATATTCTGAC CTGGGAGAAG GAGAATGAGC TGCCTGATGG ATTAGAGTGT 
GTGAAGAAAG ATGATACCTA TTACTGCATG CTTTTTAATG ATGAAGTACA CACATATGAG 
CAGGTTATAT ACACTCTTCA GAAAGCTGTC AGCTGCAGCC AGAAGGAAGC CATCAGTTTT 
GCAACAACGG TGGACAGGGA TGGGCGGAGA TCAGTTCGAA ATGGGGACTT TCAGTCTTGC 
GAGCAAGCAA AGTCGGTCAT AGTGAGGAAC ACTAGTCGAC AAACAAAACC TCTTAAAGTG 
CAGGTCATGC ATTCTGCTAT TGTTGCACAC CAGACATTTG GGTTGAAGCT CATCAGCTGG 
CTCGGCCAAG TTATTGGGTC CTCAGGTGGA CTTCGTCGCA TCCTCTGCCA GGTTGGTTTA 
CAGGAAGGTC CAGAGGGGGA AAACTCTTCG CTTGTAGACA GACTGATGTT GTGTGATTCA 
AAACTTTGGA AAGGTGCTAG AAACTGCTAT CACCAACTTT TCATGAGCAG TCTGCTAATG 
GATTTAAAGT ATAAGAAACT TTTTGCCATT CGCTTTGCAA AAAACTATGA GCGATTGCAG 
AATGATTATG TGAAAGACGA CCACGACAGG GAATTCTCTA TCACTGACCT TTCGGTACAG 
ATATTCACAG TGCCTTCACT TGCTCGAATG CTGATCACGG AAGAGAATAT GATGGCAACC 
GTAATAAAGA CATTCATGGA CCACTTGAGA ACACGGGATA TTCAGGGCAG ATTTCAGTTT 
GAACGATATA CTGCTCTACA AGCTTTCAAG TTTAGGAGAG TCCAGAGCCT CATTATGGAT 
CTGAAGTATG TGCTAATAAG CAAGCCATTT GAGTGGACAG ATGGCCTACG TTGCAAATTC 
CTAGAGGGGT TTGAAGCCTT CCTTGAATTG CTAAAATGCA TGCAGGGGAT GGACCCAATT 
ACACGCCAGG TTGGCCAGCA TATTGAGATG GAGCCAGAGT GGGAGGCAGC TTTCACTTTG 
CAGATGAAAC TAACTCACAT CATTTCAATG ATTCAAGACT GGTGTTCCTG TGATGAACAC 
GTCCTAATTG AAGCATACAA GAAGTGCCTT ATTGTCCTGA TGCAGTGCCA CAGTGGTTTT 
ACTGATGGGG AGCAACCTAT TACTCTTAGT ATATCGGGGC ATTCAGTAGA GACTATAAGA 
TACTGTGTTT CTCAGGAGAA AGTCAGCATT CACCTGCCTG TTTCACGTTT GCTTGCAGGG 
CTACATGCCT TGCTGAGTAA AAGCGAAGTA GCTTACAAGT TTCCAGAGCA GCTTCCCTTC 
AGTGAACTGA GCCCACCGAT GCTGATTGAG CATCCTCTCC GCAGTTTGGT GTTATGTGCA 
CAAGTACATG CCGGAATGTG GAGAAGAAAT GGCTTTTCAT TAGTGAATCA GATTTATTAC 
TATCACAATG TAAAATGTAG AAAAGAGATG TTTGACAAAG ATATTGTCAT GCTTCAGGCT 
GGAGCGTCTA TGATGGATCC AAACCACTTT CTAATGATTG TGCTCAGTCG TTTCGAACTG 
TATCAGATTT TTAGCACTCC AGATTACGGC AAGAGATTCC GGAAAGAACA CACAAATAAG 
GATATAATTC AACAGAATAA CACACTCATT GAAGAAATGC TGCATCTAAT TATCATGATA 
GTTGGGGAGA GATTCTGTCC TGGAGTGGGA CAAGTCAGCG GAACAGATGT GATAAAACGT 
GAAATTATCC ATCAGCTGAG CATCAAACCA ATGGCCCACA GTGAACTGGT CAAGGCTTTG 
CCCGAGGATG AAAATAAGGA AACTGGAATG GAGACTGTGA TTGAAACGAT AGCTTCATTT 
AAAAAACCAG GCCTAACAGG ACGTGGACTG TATGAACTGA AGCCTGAATG TACCAAGGAT 
TTCAACCTGT TTTACTTTCA TTTCTCAAGG GCCGAACAGT CCAAGGCAGA GGAGGCCCAG 
CGCAAACTGA AGAGGCAGAT TGGCGAAGAT ACAGCTCTCC CTCCACCGGT GCTGCCTCCG 
TTCTGTCCGC TATTTGCCAG TTTGATTAAC ATTCTTCAAT CAGATGTCAT GCTGTGCATC 
ATGGGAACTG TATTGCAATG GGCAGTAGAG CCAAATGGAT ACGCATGGTC GGAATCCATG 
CTTCAAAGGG TTTTGCATCT GATTGGGATG GCATTGCAAG AAGAGCACCA GCACTTACAG 
AATATCAGTG AGGACAACAT TGTCACCTTT AATTTCACGG AAAAGATCTC AAGGCCTGGG 
GAAGTCCCAA ATGCATCACC AAGCATCTTG GCTATGTTAG AAACATTGCA AAATGCAGCA 
CACTTGGAAG TGCATAAGGA TATGATACGC TGGATATTAA AGATGGTCAG TAACATTAAA 
AAAATCAGAG AAAATCCATC TACAAGCCCA GTCGTTGAAA CAGAGGGCAG TGTTTTGGAG 
GAGAATACTC GTGATAAAGA CAAAGCAGAG AGGAAAAGAA AAGCTGAGAT TGCCAGGCTC 
CGTAGAGAGA AGATTATGGC TCAGATGTCA GAAATGCAGC GACATTTCAT CGATGAAAAT 
AGGGAGCTTT TTCAACAGAC GACTGATGAG TTAAATGCTT CGACTTCCAC TGCATACGAT 
CACAGCTGTC ATGCAATGAT GGACACCAAA CTTGTCGCTT TGGGTCCTCA CCAAACCCGA 
ATCAGTGAAC CAAGGCAGCT GGTTACTTGC ATCCTGTGTC AAGAAGAGCA AGCTTTCACA 
GTGGACAACA AAGCCATGGT TCTTGCAGCT TTTATTCAGA GATCGACGGT ATTCTCCAAG 
AACAGGAGCA GAGTCATTAC AGATCCAGAA AAATATGACC CGCTGTGCAT GCACCCAGAC 
TTGGCTTGTG GCACTCACAC AGGAAGCTGT GGACATATAA TGCACGCTCA CTGCTGGCAG 
AGGTACTTTG ATGCTGTGCA AGCCAAGGAG CAGAGAAGGC AGCAGCGCTT ACGGGTACAT 
ACTAGCTACG ATGTGGAAAA TGGTGAATTT CTCTGCCCCC TCTGTGAGTG TCTAAGTAAC 
ACCGTCATCC CCATGCTTCC ACCACCAAGA AGTTTATACC ACAGGTTGGA TTTCTCTGAT 
CAGCCTGATT TAGCAGAATG GGTTAGCACT ACTTCTCAGC AACTCAAAGC ATTGTGTGCG 
CTGCATGCAG AGAAAAGCAG TTCAGCTGGT TCAGATTCCG AGATGCCAGC ACAAGTGGAT 
ATTCCTGAAG GTTTCCGGCC TGATTTCCAA CCAAAAAATC CATACTCAGA GAGCATTAGG 
GAGATGCTGA CAACATTCGG GACGGCAACA TATAAGGTTG GGCTGAATGT GCACCCAAAC 
GAGCAGGACC CAAGGGTCCC AATTATGTGC TGGGGAAGCT GTGCGTACAC GATACAAACA 
ATTGAACGCA TTTTGGCAGA TGAAGAGAGG CCGTTATTTG GGAACTTGCC ATGCAGACAG 
GATGACTGCC TAAAATCACT GAGCAGATTT GCTGCAGCAC ATTGGACAGT GTGCCCTCTT 
CCTGTGATGG AAAAACACTT TAGTCGTCTA TTTGAAACAA TTGTGCCTAA TGAGGATGCC 
GAGAATTCCC CATGCATACT TGATATTGAT ATGTTTCATT TACTGGTCAG CTTGGTGGTC 
TCATTCCCAG CTCTACAGTG TCAGGACTTT TCAGGGAACA GCCTTGCTAC AGGAGATCTC 
CATCTTTTCC ATCTGGTGAC CATGGCACAC ATTGTCCAGA TAGTGCTTTC CTGCTCCACA 
GAGGAAAATG GCATGGAGCA GGAATCCACT GATGGAGAAG AAGAACAGGC AGCAATGTCT 
TTGTATAGGA CCATCTGTCG GTGCAGTGGA AGCACAATGA AAGAATTATC CTCTGGGTGG 
CACCTATGCA GAGCAATCAA AACTTCAATC CTGCCGTACC TGAGGTGTGC TGCGTTATTT 
TTCCATTATT TAAATGGTGT TCCAGCACCT TCAGAGTTTC TCGTGAGTGG AATGAGCCAG 
TTTGATTGTC TCTGTAGTTA CCTTTCTTTG CCAACCAACT TGGTCTGCCT GTTTCAAGAG 
AATTCTAAAG TAATGGAAAC ATTAATAAAC AGATGGTGTA AACATGCAGA GGTGAAAAGG 
TTTCTGAATG GGGAACGCAC TGCTGTTAGC TATCCAAGGG AATCAAACAA ACTTATCGAT 
CTTCCAGAGG ACTACAGCAG CCTAATTAAT CAAGCATCTA ATTTCACGTG CCCTAAATCT 
GGTGGAGACA AAAGCAGGGC CCCTACGCTC TGCCTGGTGT GTGGAACCAT GCTGTGCTCC 
CAGAGTTACT GCTGCCAGAC AGAACTGGAT GGGGACGACG TGGGGGCCTG CACTGCCCAT 
ACCTACACGT GTGGATCTGG CATGGGCATT TTCCTTCGAG TCAGAGAGTG TCAAGTTCTC 
TTTTTGGCTG GGAAAACAAA GGGCTGCTTC TATGCTCCTC CTTACTTAGA TGACTATGGA 
GAGACAGATC AAGGCCTCCG ACGCGGAAAC CCCCTGCACC TCTGTAAGGA ACGCTACAAA 
AAGATCCAGA AGCTGTGGCA GCAGCACAGT ATTACAGAGG AAATTGGCCA TGCCCAAGAA 
GCCAATCAGA CTCTAACTGG CATTGAATGG CAGCATTTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ubr2 ( XM_004914083.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914140.1 Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X2, mRNA.

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CloneID	OXa09643
Clone ID Related Accession (Same CDS sequence)	XM_004914083.3
Accession Version	XM_004914083.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase UBR2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004914083.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTC CAGAGCAAGA AGCTGAAGTG CCAGGAATAG ACAGCGAGTT GTTGGCCTGC 
TGTGCCGGGG AGGTGGCCTT GAAATGGCTC CAAGCAACTG ATCTACCTAA AGAAGTTTAT 
CAGCACCTTG CCTACTATGT ACCCAAAATT TACTGTCAAG GCTCCAACCC AACTCCAGAA 
AATGAGGACA TGGTGGTCCA GCACGTTCTG TTGGGCCCTA TGGAGTGGTA TCTGTGCGGC 
GAGGACCCTG CAATTGGATT TCCAAAGCTT AAGCAAGCCA ACAAGCCATC TCAGCTGTGT 
GGCCGGGTAT TTAAAGTGGG AGAGCCAACA TATTCATGCA GGGACTGTGC AGTCGACCCA 
ACATGCGTTT TATGCATGGA GTGTTTTTTA GGAAGTGTTC ATAGAGAGCA CCGATATCGG 
ATGACAACGT CAGGGGGTGG AGGATTCTGT GATTGTGGAG ATGCTGAAGC ATGGAAAGGA 
GGGCCATACT GCCAGAAACA TGCACTGAAT ATCTCCGAAA GTGAGACAGA GGACCCACTA 
ATTCATTTGT CAGAAGATAT GATAGCAAGA GCATATAATA TGTTTGCCAT TATGTTTCGC 
TACGCAGTGG ATATTCTGAC CTGGGAGAAG GAGAATGAGC TGCCTGATGG ATTAGAGTGT 
GTGAAGAAAG ATGATACCTA TTACTGCATG CTTTTTAATG ATGAAGTACA CACATATGAG 
CAGGTTATAT ACACTCTTCA GAAAGCTGTC AGCTGCAGCC AGAAGGAAGC CATCAGTTTT 
GCAACAACGG TGGACAGGGA TGGGCGGAGA TCAGTTCGAA ATGGGGACTT TCAGTCTTGC 
GAGCAAGCAA AGTCGGTCAT AGTGAGGAAC ACTAGTCGAC AAACAAAACC TCTTAAAGTG 
CAGGTCATGC ATTCTGCTAT TGTTGCACAC CAGACATTTG GGTTGAAGCT CATCAGCTGG 
CTCGGCCAAG TTATTGGGTC CTCAGGTGGA CTTCGTCGCA TCCTCTGCCA GGTTGGTTTA 
CAGGAAGGTC CAGAGGGGGA AAACTCTTCG CTTGTAGACA GACTGATGTT GTGTGATTCA 
AAACTTTGGA AAGGTGCTAG AAACTGCTAT CACCAACTTT TCATGAGCAG TCTGCTAATG 
GATTTAAAGT ATAAGAAACT TTTTGCCATT CGCTTTGCAA AAAATTACCA GCAGTTGCAG 
AGAGATTTTA TGGAGGATGA TCACGATCGA GCAGTATCGG TGGCTGCTCT GTCTGTTCAG 
CTCTTCACTG TACCCACTCT GGCTCGAATG CTGATCACGG AAGAGAATAT GATGGCAACC 
GTAATAAAGA CATTCATGGA CCACTTGAGA ACACGGGATA TTCAGGGCAG ATTTCAGTTT 
GAACGATATA CTGCTCTACA AGCTTTCAAG TTTAGGAGAG TCCAGAGCCT CATTATGGAT 
CTGAAGTATG TGCTAATAAG CAAGCCATTT GAGTGGACAG ATGGCCTACG TTGCAAATTC 
CTAGAGGGGT TTGAAGCCTT CCTTGAATTG CTAAAATGCA TGCAGGGGAT GGACCCAATT 
ACACGCCAGG TTGGCCAGCA TATTGAGATG GAGCCAGAGT GGGAGGCAGC TTTCACTTTG 
CAGATGAAAC TAACTCACAT CATTTCAATG ATTCAAGACT GGTGTTCCTG TGATGAACAC 
GTCCTAATTG AAGCATACAA GAAGTGCCTT ATTGTCCTGA TGCAGTGCCA CAGTGGTTTT 
ACTGATGGGG AGCAACCTAT TACTCTTAGT ATATCGGGGC ATTCAGTAGA GACTATAAGA 
TACTGTGTTT CTCAGGAGAA AGTCAGCATT CACCTGCCTG TTTCACGTTT GCTTGCAGGG 
CTACATGCCT TGCTGAGTAA AAGCGAAGTA GCTTACAAGT TTCCAGAGCA GCTTCCCTTC 
AGTGAACTGA GCCCACCGAT GCTGATTGAG CATCCTCTCC GCAGTTTGGT GTTATGTGCA 
CAAGTACATG CCGGAATGTG GAGAAGAAAT GGCTTTTCAT TAGTGAATCA GATTTATTAC 
TATCACAATG TAAAATGTAG AAAAGAGATG TTTGACAAAG ATATTGTCAT GCTTCAGGCT 
GGAGCGTCTA TGATGGATCC AAACCACTTT CTAATGATTG TGCTCAGTCG TTTCGAACTG 
TATCAGATTT TTAGCACTCC AGATTACGGC AAGAGATTCC GGAAAGAACA CACAAATAAG 
GATATAATTC AACAGAATAA CACACTCATT GAAGAAATGC TGCATCTAAT TATCATGATA 
GTTGGGGAGA GATTCTGTCC TGGAGTGGGA CAAGTCAGCG GAACAGATGT GATAAAACGT 
GAAATTATCC ATCAGCTGAG CATCAAACCA ATGGCCCACA GTGAACTGGT CAAGGCTTTG 
CCCGAGGATG AAAATAAGGA AACTGGAATG GAGACTGTGA TTGAAACGAT AGCTTCATTT 
AAAAAACCAG GCCTAACAGG ACGTGGACTG TATGAACTGA AGCCTGAATG TACCAAGGAT 
TTCAACCTGT TTTACTTTCA TTTCTCAAGG GCCGAACAGT CCAAGGCAGA GGAGGCCCAG 
CGCAAACTGA AGAGGCAGAT TGGCGAAGAT ACAGCTCTCC CTCCACCGGT GCTGCCTCCG 
TTCTGTCCGC TATTTGCCAG TTTGATTAAC ATTCTTCAAT CAGATGTCAT GCTGTGCATC 
ATGGGAACTG TATTGCAATG GGCAGTAGAG CCAAATGGAT ACGCATGGTC GGAATCCATG 
CTTCAAAGGG TTTTGCATCT GATTGGGATG GCATTGCAAG AAGAGCACCA GCACTTACAG 
AATATCAGTG AGGACAACAT TGTCACCTTT AATTTCACGG AAAAGATCTC AAGGCCTGGG 
GAAGTCCCAA ATGCATCACC AAGCATCTTG GCTATGTTAG AAACATTGCA AAATGCAGCA 
CACTTGGAAG TGCATAAGGA TATGATACGC TGGATATTAA AGATGGTCAG TAACATTAAA 
AAAATCAGAG AAAATCCATC TACAAGCCCA GTCGTTGAAA CAGAGGGCAG TGTTTTGGAG 
GAGAATACTC GTGATAAAGA CAAAGCAGAG AGGAAAAGAA AAGCTGAGAT TGCCAGGCTC 
CGTAGAGAGA AGATTATGGC TCAGATGTCA GAAATGCAGC GACATTTCAT CGATGAAAAT 
AGGGAGCTTT TTCAACAGAC GACTGATGAG TTAAATGCTT CGACTTCCAC TGCATACGAT 
CACAGCTGTC ATGCAATGAT GGACACCAAA CTTGTCGCTT TGGGTCCTCA CCAAACCCGA 
ATCAGTGAAC CAAGGCAGCT GGTTACTTGC ATCCTGTGTC AAGAAGAGCA AGCTTTCACA 
GTGGACAACA AAGCCATGGT TCTTGCAGCT TTTATTCAGA GATCGACGGT ATTCTCCAAG 
AACAGGAGCA GAGTCATTAC AGATCCAGAA AAATATGACC CGCTGTGCAT GCACCCAGAC 
TTGGCTTGTG GCACTCACAC AGGAAGCTGT GGACATATAA TGCACGCTCA CTGCTGGCAG 
AGGTACTTTG ATGCTGTGCA AGCCAAGGAG CAGAGAAGGC AGCAGCGCTT ACGGGTACAT 
ACTAGCTACG ATGTGGAAAA TGGTGAATTT CTCTGCCCCC TCTGTGAGTG TCTAAGTAAC 
ACCGTCATCC CCATGCTTCC ACCACCAAGA AGTTTATACC ACAGGTTGGA TTTCTCTGAT 
CAGCCTGATT TAGCAGAATG GGTTAGCACT ACTTCTCAGC AACTCAAAGC ATTGTGTGCG 
CTGCATGCAG AGAAAAGCAG TTCAGCTGGT TCAGATTCCG AGATGCCAGC ACAAGTGGAT 
ATTCCTGAAG GTTTCCGGCC TGATTTCCAA CCAAAAAATC CATACTCAGA GAGCATTAGG 
GAGATGCTGA CAACATTCGG GACGGCAACA TATAAGGTTG GGCTGAATGT GCACCCAAAC 
GAGCAGGACC CAAGGGTCCC AATTATGTGC TGGGGAAGCT GTGCGTACAC GATACAAACA 
ATTGAACGCA TTTTGGCAGA TGAAGAGAGG CCGTTATTTG GGAACTTGCC ATGCAGACAG 
GATGACTGCC TAAAATCACT GAGCAGATTT GCTGCAGCAC ATTGGACAGT GTGCCCTCTT 
CCTGTGATGG AAAAACACTT TAGTCGTCTA TTTGAAACAA TTGTGCCTAA TGAGGATGCC 
GAGAATTCCC CATGCATACT TGATATTGAT ATGTTTCATT TACTGGTCAG CTTGGTGGTC 
TCATTCCCAG CTCTACAGTG TCAGGACTTT TCAGGGAACA GCCTTGCTAC AGGAGATCTC 
CATCTTTTCC ATCTGGTGAC CATGGCACAC ATTGTCCAGA TAGTGCTTTC CTGCTCCACA 
GAGGAAAATG GCATGGAGCA GGAATCCACT GATGGAGAAG AAGAACAGGC AGCAATGTCT 
TTGTATAGGA CCATCTGTCG GTGCAGTGGA AGCACAATGA AAGAATTATC CTCTGGGTGG 
CACCTATGCA GAGCAATCAA AACTTCAATC CTGCCGTACC TGAGGTGTGC TGCGTTATTT 
TTCCATTATT TAAATGGTGT TCCAGCACCT TCAGAGTTTC TCGTGAGTGG AATGAGCCAG 
TTTGATTGTC TCTGTAGTTA CCTTTCTTTG CCAACCAACT TGGTCTGCCT GTTTCAAGAG 
AATTCTAAAG TAATGGAAAC ATTAATAAAC AGATGGTGTA AACATGCAGA GGTGAAAAGG 
TTTCTGAATG GGGAACGCAC TGCTGTTAGC TATCCAAGGG AATCAAACAA ACTTATCGAT 
CTTCCAGAGG ACTACAGCAG CCTAATTAAT CAAGCATCTA ATTTCACGTG CCCTAAATCT 
GGTGGAGACA AAAGCAGGGC CCCTACGCTC TGCCTGGTGT GTGGAACCAT GCTGTGCTCC 
CAGAGTTACT GCTGCCAGAC AGAACTGGAT GGGGACGACG TGGGGGCCTG CACTGCCCAT 
ACCTACACGT GTGGATCTGG CATGGGCATT TTCCTTCGAG TCAGAGAGTG TCAAGTTCTC 
TTTTTGGCTG GGAAAACAAA GGGCTGCTTC TATGCTCCTC CTTACTTAGA TGACTATGGA 
GAGACAGATC AAGGCCTCCG ACGCGGAAAC CCCCTGCACC TCTGTAAGGA ACGCTACAAA 
AAGATCCAGA AGCTGTGGCA GCAGCACAGT ATTACAGAGG AAATTGGCCA TGCCCAAGAA 
GCCAATCAGA CTCTAACTGG CATTGAATGG CAGCATTTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914140.1
CDS	74..5335
Translation	MAAPEQEAEVPGIDSELLACCAGEVALKWLQATDLPKEVYQHLAYYVPKIYCQGSNPTPENEDMVVQHVLLGPMEWYLCGEDPAIGFPKLKQANKPSQLCGRVFKVGEPTYSCRDCAVDPTCVLCMECFLGSVHREHRYRMTTSGGGGFCDCGDAEAWKGGPYCQKHALNISESETEDPLIHLSEDMIARAYNMFAIMFRYAVDILTWEKENELPDGLECVKKDDTYYCMLFNDEVHTYEQVIYTLQKAVSCSQKEAISFATTVDRDGRRSVRNGDFQSCEQAKSVIVRNTSRQTKPLKVQVMHSAIVAHQTFGLKLISWLGQVIGSSGGLRRILCQVGLQEGPEGENSSLVDRLMLCDSKLWKGARNCYHQLFMSSLLMDLKYKKLFAIRFAKNYQQLQRDFMEDDHDRAVSVAALSVQLFTVPTLARMLITEENMMATVIKTFMDHLRTRDIQGRFQFERYTALQAFKFRRVQSLIMDLKYVLISKPFEWTDGLRCKFLEGFEAFLELLKCMQGMDPITRQVGQHIEMEPEWEAAFTLQMKLTHIISMIQDWCSCDEHVLIEAYKKCLIVLMQCHSGFTDGEQPITLSISGHSVETIRYCVSQEKVSIHLPVSRLLAGLHALLSKSEVAYKFPEQLPFSELSPPMLIEHPLRSLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRKEMFDKDIVMLQAGASMMDPNHFLMIVLSRFELYQIFSTPDYGKRFRKEHTNKDIIQQNNTLIEEMLHLIIMIVGERFCPGVGQVSGTDVIKREIIHQLSIKPMAHSELVKALPEDENKETGMETVIETIASFKKPGLTGRGLYELKPECTKDFNLFYFHFSRAEQSKAEEAQRKLKRQIGEDTALPPPVLPPFCPLFASLINILQSDVMLCIMGTVLQWAVEPNGYAWSESMLQRVLHLIGMALQEEHQHLQNISEDNIVTFNFTEKISRPGEVPNASPSILAMLETLQNAAHLEVHKDMIRWILKMVSNIKKIRENPSTSPVVETEGSVLEENTRDKDKAERKRKAEIARLRREKIMAQMSEMQRHFIDENRELFQQTTDELNASTSTAYDHSCHAMMDTKLVALGPHQTRISEPRQLVTCILCQEEQAFTVDNKAMVLAAFIQRSTVFSKNRSRVITDPEKYDPLCMHPDLACGTHTGSCGHIMHAHCWQRYFDAVQAKEQRRQQRLRVHTSYDVENGEFLCPLCECLSNTVIPMLPPPRSLYHRLDFSDQPDLAEWVSTTSQQLKALCALHAEKSSSAGSDSEMPAQVDIPEGFRPDFQPKNPYSESIREMLTTFGTATYKVGLNVHPNEQDPRVPIMCWGSCAYTIQTIERILADEERPLFGNLPCRQDDCLKSLSRFAAAHWTVCPLPVMEKHFSRLFETIVPNEDAENSPCILDIDMFHLLVSLVVSFPALQCQDFSGNSLATGDLHLFHLVTMAHIVQIVLSCSTEENGMEQESTDGEEEQAAMSLYRTICRCSGSTMKELSSGWHLCRAIKTSILPYLRCAALFFHYLNGVPAPSEFLVSGMSQFDCLCSYLSLPTNLVCLFQENSKVMETLINRWCKHAEVKRFLNGERTAVSYPRESNKLIDLPEDYSSLINQASNFTCPKSGGDKSRAPTLCLVCGTMLCSQSYCCQTELDGDDVGACTAHTYTCGSGMGIFLRVRECQVLFLAGKTKGCFYAPPYLDDYGETDQGLRRGNPLHLCKERYKKIQKLWQQHSITEEIGHAQEANQTLTGIEWQHL

Target ORF information:

RefSeq Version	XM_004914083.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914083.3

ORF Insert Sequence:

ATGGCGGCTC CAGAGCAAGA AGCTGAAGTG CCAGGAATAG ACAGCGAGTT GTTGGCCTGC 
TGTGCCGGGG AGGTGGCCTT GAAATGGCTC CAAGCAACTG ATCTACCTAA AGAAGTTTAT 
CAGCACCTTG CCTACTATGT ACCCAAAATT TACTGTCAAG GCTCCAACCC AACTCCAGAA 
AATGAGGACA TGGTGGTCCA GCACGTTCTG TTGGGCCCTA TGGAGTGGTA TCTGTGCGGC 
GAGGACCCTG CAATTGGATT TCCAAAGCTT AAGCAAGCCA ACAAGCCATC TCAGCTGTGT 
GGCCGGGTAT TTAAAGTGGG AGAGCCAACA TATTCATGCA GGGACTGTGC AGTCGACCCA 
ACATGCGTTT TATGCATGGA GTGTTTTTTA GGAAGTGTTC ATAGAGAGCA CCGATATCGG 
ATGACAACGT CAGGGGGTGG AGGATTCTGT GATTGTGGAG ATGCTGAAGC ATGGAAAGGA 
GGGCCATACT GCCAGAAACA TGCACTGAAT ATCTCCGAAA GTGAGACAGA GGACCCACTA 
ATTCATTTGT CAGAAGATAT GATAGCAAGA GCATATAATA TGTTTGCCAT TATGTTTCGC 
TACGCAGTGG ATATTCTGAC CTGGGAGAAG GAGAATGAGC TGCCTGATGG ATTAGAGTGT 
GTGAAGAAAG ATGATACCTA TTACTGCATG CTTTTTAATG ATGAAGTACA CACATATGAG 
CAGGTTATAT ACACTCTTCA GAAAGCTGTC AGCTGCAGCC AGAAGGAAGC CATCAGTTTT 
GCAACAACGG TGGACAGGGA TGGGCGGAGA TCAGTTCGAA ATGGGGACTT TCAGTCTTGC 
GAGCAAGCAA AGTCGGTCAT AGTGAGGAAC ACTAGTCGAC AAACAAAACC TCTTAAAGTG 
CAGGTCATGC ATTCTGCTAT TGTTGCACAC CAGACATTTG GGTTGAAGCT CATCAGCTGG 
CTCGGCCAAG TTATTGGGTC CTCAGGTGGA CTTCGTCGCA TCCTCTGCCA GGTTGGTTTA 
CAGGAAGGTC CAGAGGGGGA AAACTCTTCG CTTGTAGACA GACTGATGTT GTGTGATTCA 
AAACTTTGGA AAGGTGCTAG AAACTGCTAT CACCAACTTT TCATGAGCAG TCTGCTAATG 
GATTTAAAGT ATAAGAAACT TTTTGCCATT CGCTTTGCAA AAAATTACCA GCAGTTGCAG 
AGAGATTTTA TGGAGGATGA TCACGATCGA GCAGTATCGG TGGCTGCTCT GTCTGTTCAG 
CTCTTCACTG TACCCACTCT GGCTCGAATG CTGATCACGG AAGAGAATAT GATGGCAACC 
GTAATAAAGA CATTCATGGA CCACTTGAGA ACACGGGATA TTCAGGGCAG ATTTCAGTTT 
GAACGATATA CTGCTCTACA AGCTTTCAAG TTTAGGAGAG TCCAGAGCCT CATTATGGAT 
CTGAAGTATG TGCTAATAAG CAAGCCATTT GAGTGGACAG ATGGCCTACG TTGCAAATTC 
CTAGAGGGGT TTGAAGCCTT CCTTGAATTG CTAAAATGCA TGCAGGGGAT GGACCCAATT 
ACACGCCAGG TTGGCCAGCA TATTGAGATG GAGCCAGAGT GGGAGGCAGC TTTCACTTTG 
CAGATGAAAC TAACTCACAT CATTTCAATG ATTCAAGACT GGTGTTCCTG TGATGAACAC 
GTCCTAATTG AAGCATACAA GAAGTGCCTT ATTGTCCTGA TGCAGTGCCA CAGTGGTTTT 
ACTGATGGGG AGCAACCTAT TACTCTTAGT ATATCGGGGC ATTCAGTAGA GACTATAAGA 
TACTGTGTTT CTCAGGAGAA AGTCAGCATT CACCTGCCTG TTTCACGTTT GCTTGCAGGG 
CTACATGCCT TGCTGAGTAA AAGCGAAGTA GCTTACAAGT TTCCAGAGCA GCTTCCCTTC 
AGTGAACTGA GCCCACCGAT GCTGATTGAG CATCCTCTCC GCAGTTTGGT GTTATGTGCA 
CAAGTACATG CCGGAATGTG GAGAAGAAAT GGCTTTTCAT TAGTGAATCA GATTTATTAC 
TATCACAATG TAAAATGTAG AAAAGAGATG TTTGACAAAG ATATTGTCAT GCTTCAGGCT 
GGAGCGTCTA TGATGGATCC AAACCACTTT CTAATGATTG TGCTCAGTCG TTTCGAACTG 
TATCAGATTT TTAGCACTCC AGATTACGGC AAGAGATTCC GGAAAGAACA CACAAATAAG 
GATATAATTC AACAGAATAA CACACTCATT GAAGAAATGC TGCATCTAAT TATCATGATA 
GTTGGGGAGA GATTCTGTCC TGGAGTGGGA CAAGTCAGCG GAACAGATGT GATAAAACGT 
GAAATTATCC ATCAGCTGAG CATCAAACCA ATGGCCCACA GTGAACTGGT CAAGGCTTTG 
CCCGAGGATG AAAATAAGGA AACTGGAATG GAGACTGTGA TTGAAACGAT AGCTTCATTT 
AAAAAACCAG GCCTAACAGG ACGTGGACTG TATGAACTGA AGCCTGAATG TACCAAGGAT 
TTCAACCTGT TTTACTTTCA TTTCTCAAGG GCCGAACAGT CCAAGGCAGA GGAGGCCCAG 
CGCAAACTGA AGAGGCAGAT TGGCGAAGAT ACAGCTCTCC CTCCACCGGT GCTGCCTCCG 
TTCTGTCCGC TATTTGCCAG TTTGATTAAC ATTCTTCAAT CAGATGTCAT GCTGTGCATC 
ATGGGAACTG TATTGCAATG GGCAGTAGAG CCAAATGGAT ACGCATGGTC GGAATCCATG 
CTTCAAAGGG TTTTGCATCT GATTGGGATG GCATTGCAAG AAGAGCACCA GCACTTACAG 
AATATCAGTG AGGACAACAT TGTCACCTTT AATTTCACGG AAAAGATCTC AAGGCCTGGG 
GAAGTCCCAA ATGCATCACC AAGCATCTTG GCTATGTTAG AAACATTGCA AAATGCAGCA 
CACTTGGAAG TGCATAAGGA TATGATACGC TGGATATTAA AGATGGTCAG TAACATTAAA 
AAAATCAGAG AAAATCCATC TACAAGCCCA GTCGTTGAAA CAGAGGGCAG TGTTTTGGAG 
GAGAATACTC GTGATAAAGA CAAAGCAGAG AGGAAAAGAA AAGCTGAGAT TGCCAGGCTC 
CGTAGAGAGA AGATTATGGC TCAGATGTCA GAAATGCAGC GACATTTCAT CGATGAAAAT 
AGGGAGCTTT TTCAACAGAC GACTGATGAG TTAAATGCTT CGACTTCCAC TGCATACGAT 
CACAGCTGTC ATGCAATGAT GGACACCAAA CTTGTCGCTT TGGGTCCTCA CCAAACCCGA 
ATCAGTGAAC CAAGGCAGCT GGTTACTTGC ATCCTGTGTC AAGAAGAGCA AGCTTTCACA 
GTGGACAACA AAGCCATGGT TCTTGCAGCT TTTATTCAGA GATCGACGGT ATTCTCCAAG 
AACAGGAGCA GAGTCATTAC AGATCCAGAA AAATATGACC CGCTGTGCAT GCACCCAGAC 
TTGGCTTGTG GCACTCACAC AGGAAGCTGT GGACATATAA TGCACGCTCA CTGCTGGCAG 
AGGTACTTTG ATGCTGTGCA AGCCAAGGAG CAGAGAAGGC AGCAGCGCTT ACGGGTACAT 
ACTAGCTACG ATGTGGAAAA TGGTGAATTT CTCTGCCCCC TCTGTGAGTG TCTAAGTAAC 
ACCGTCATCC CCATGCTTCC ACCACCAAGA AGTTTATACC ACAGGTTGGA TTTCTCTGAT 
CAGCCTGATT TAGCAGAATG GGTTAGCACT ACTTCTCAGC AACTCAAAGC ATTGTGTGCG 
CTGCATGCAG AGAAAAGCAG TTCAGCTGGT TCAGATTCCG AGATGCCAGC ACAAGTGGAT 
ATTCCTGAAG GTTTCCGGCC TGATTTCCAA CCAAAAAATC CATACTCAGA GAGCATTAGG 
GAGATGCTGA CAACATTCGG GACGGCAACA TATAAGGTTG GGCTGAATGT GCACCCAAAC 
GAGCAGGACC CAAGGGTCCC AATTATGTGC TGGGGAAGCT GTGCGTACAC GATACAAACA 
ATTGAACGCA TTTTGGCAGA TGAAGAGAGG CCGTTATTTG GGAACTTGCC ATGCAGACAG 
GATGACTGCC TAAAATCACT GAGCAGATTT GCTGCAGCAC ATTGGACAGT GTGCCCTCTT 
CCTGTGATGG AAAAACACTT TAGTCGTCTA TTTGAAACAA TTGTGCCTAA TGAGGATGCC 
GAGAATTCCC CATGCATACT TGATATTGAT ATGTTTCATT TACTGGTCAG CTTGGTGGTC 
TCATTCCCAG CTCTACAGTG TCAGGACTTT TCAGGGAACA GCCTTGCTAC AGGAGATCTC 
CATCTTTTCC ATCTGGTGAC CATGGCACAC ATTGTCCAGA TAGTGCTTTC CTGCTCCACA 
GAGGAAAATG GCATGGAGCA GGAATCCACT GATGGAGAAG AAGAACAGGC AGCAATGTCT 
TTGTATAGGA CCATCTGTCG GTGCAGTGGA AGCACAATGA AAGAATTATC CTCTGGGTGG 
CACCTATGCA GAGCAATCAA AACTTCAATC CTGCCGTACC TGAGGTGTGC TGCGTTATTT 
TTCCATTATT TAAATGGTGT TCCAGCACCT TCAGAGTTTC TCGTGAGTGG AATGAGCCAG 
TTTGATTGTC TCTGTAGTTA CCTTTCTTTG CCAACCAACT TGGTCTGCCT GTTTCAAGAG 
AATTCTAAAG TAATGGAAAC ATTAATAAAC AGATGGTGTA AACATGCAGA GGTGAAAAGG 
TTTCTGAATG GGGAACGCAC TGCTGTTAGC TATCCAAGGG AATCAAACAA ACTTATCGAT 
CTTCCAGAGG ACTACAGCAG CCTAATTAAT CAAGCATCTA ATTTCACGTG CCCTAAATCT 
GGTGGAGACA AAAGCAGGGC CCCTACGCTC TGCCTGGTGT GTGGAACCAT GCTGTGCTCC 
CAGAGTTACT GCTGCCAGAC AGAACTGGAT GGGGACGACG TGGGGGCCTG CACTGCCCAT 
ACCTACACGT GTGGATCTGG CATGGGCATT TTCCTTCGAG TCAGAGAGTG TCAAGTTCTC 
TTTTTGGCTG GGAAAACAAA GGGCTGCTTC TATGCTCCTC CTTACTTAGA TGACTATGGA 
GAGACAGATC AAGGCCTCCG ACGCGGAAAC CCCCTGCACC TCTGTAAGGA ACGCTACAAA 
AAGATCCAGA AGCTGTGGCA GCAGCACAGT ATTACAGAGG AAATTGGCCA TGCCCAAGAA 
GCCAATCAGA CTCTAACTGG CATTGAATGG CAGCATTTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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ubr2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

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