trio cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following trio gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the trio cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa01813 NM_001129921.1
Latest version!
Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa32253 XM_012964353.3
Latest version!
Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa32254 XM_012964354.3
Latest version!
Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa32255 XM_012964355.3
Latest version!
Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa32256 XM_012964356.2
Latest version!
Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa44862 XM_018094560.1
Latest version!
Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa59535 XM_031903241.1
Latest version!
Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
View Old Accession Versions >>
OXa32253 XM_012964353.2 Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa32255 XM_012964355.2 Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa32254 XM_012964354.2 Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa01813
    Clone ID Related Accession (Same CDS sequence) NM_001129921.1
    Accession Version NM_001129921.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9147bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Xenopus tropicalis(tropical clawed frog)
    Product triple functional domain protein
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC167460.1. ##Evidence-Data-START## Transcript exon combination :: BC167460.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028309, SAMD00028310 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGCAGACTC ATCGCTGGAA AGATGACGGA GAAACAACAT TAGGTTTTCG TAGAAATGAT 
    GAATTGAAAG CAATGGAGGT GTTGCCAATT TTAAAAGAAA AAGTGGCATA TCTATCAGGT
    GGCAGAGATA AACGAGGTGG TCCTGTATTA ACATTTCCAG CACGCAGCAA CCATGATCGA
    ATACGACAAG AGGATCTCCG AAGACTGATT TCATATCTTG CTTGTATTCC TAGTGAGGAG
    GTGTGCAAGC GAGGCTTCAC AGTGATTGTA GATATGCGTG GTTCCAAGTG GGATTCTATT
    AAACCCTTGC TGAAAATTTT ACAAGAATCC TTTCCATGCT GTATTCATGT TGCACTAATT
    ATCAAACCAG ATAACTTCTG GCAGAAGCAA AGAACTAATT TTGGAAGTTC TAAGTTTGAA
    TTTGAGACAA GTATGGTGTC TTTAGAAGGC CTTACCAAAG TAGTTGACCC TTCCCAGCTA
    ACTCCAGAAT TTGAGGGTTG CTTGGAATAC AACCATGAAG AGTGGATAGA GATCAGAGTA
    GCATTTGAGG ATTTTATTAA TAATGCAACT CATATGCTGT CTCGATTAGA GGAATTACAG
    GAGATGCTTG CAATTAAAGA GTTGCCTCAG GATTTAGAGG GGGCACGCAA TATGATTGAA
    GAACATTCTC AAATGAAAAA AAGGGTTATA AAGGCTCCTA TTGAAGATCT TGATTTAGAA
    GGGCAAAAGC TTTTACAAAC AATACAAAGC AGTGATAGCT TTCCTAAGAA GAACTCTAGT
    TCTGGAAATG CAGACCTTCA AAATCTTCTT CCCAAAGTTT CCACAATGTT AGACAGGCTT
    CATTCAACAC GACAACATCT TCATCAGATG TGGCACATGC GAAAAGTAAA ACTGGACCAG
    TGCTTTCAGC TAAGGTTGTT TGAGCAGGAT GCTGAGAAGA TGTTTGACTG GATTACACAC
    AACAAAGGTC TGTTTCTGAC AAGTTATACT GACATTGGCA CCCGTAATAA TCATGCTATG
    GAACTTCAGA CACAGCACAA TCATTTTGCT ATGAACTGCA TGAATGTCTA TGTAAACATT
    AGCCGAATAA TGTCTGTGGC AAATCGACTA GTAGAATCTG GACACTATGC CTCACAGCAA
    ATTAAGCAGA TAGCTAACCA GTTGGAGCAA GAGTGGAAGG CTTTTGCCGC AGCCCTTGAT
    GAACGTAGTA CGTTGCTTGA CATGTCTGCA ATGTTCCATC AAAAAGCGGA GCAGTACATG
    GGCAATGTGG ATTCATGGTG CAAAGCTTGT GGGGAAGTGG ATCTTCCATC AGAGCTGCAG
    GATCTAGAAG ATGCCATTCA TCACCATCAG GGAATATATG AGCATATCAC TTTAGCATAT
    TCAGAGGTTA GCCAGGATGG TAAATCTTTA TTAGACAAAC TCCAGAGACC ATTGACTCCT
    GGTAGTTCTG ACTCACTGAC AGCTTCTGCC AACTATTCAA AGGCTGTGCA TCACGTCTTA
    GATGCAATAC ATGAAGTCTT GCACCACCAG CGTCAGTTGG AAAACATCTG GCAACATCGC
    AAAGTTCGTC TGCATCAACG CCTACAGCTC TGTGTTTTTC AGCAAGATGT TCAGCAGGTC
    TTGGATTGGA TAGAGAACCA TGGTGAAGCC TTTCTCAGCA AACACACTGG GGTAGGCAAG
    TCTCTGCACA GGGCCAGAGC CTTGCAAAAG CGACATGAAG ACTTTGAAGA AGTCGCTCAG
    AATACATACA CCAATGCTGA TAAGCTGCTG GAAGCTGCAG AACAGTTGGC CCAGACAGGA
    GAGTGCGATC CAGAGGAAAT ATACCAGGCT GCTCATCAGC TTGAGGATCG GATTCAGGAT
    TTTGTAAGAC GTGTAGAACA AAGAAAAGTT CTCTTGGACA TGTCTGTATC ATTCCACTCG
    CATGTTAAAG AGCTATGGAC ATGGCTGGAG GACCTACAAA AGGAACTGCT GGATGATGTC
    TATGCGGAGT CAGTAGAAGC TGTACAGGAC TTGATCAAAC GGTTTGGACA ACAGCAGCAG
    ACAACCCTCC AGTTTACAGT AAATGTCATC AAAGAAGGGG AGGATCTAAT TCAGCAACTA
    AGAGATTCTG CTATATCCAG CAACAAAACT CCTCACAACA GCTCAATCAA TCACATTGAG
    ACTGTCCTTC AACAACTTGA TGAGGCCCAA TCACAGATGG AGGAGCTTTT CCAAGAGAGA
    AAAATCAAAC TTGAGCTATT CTTGCAGCTT CGCATCTTTG AGAGAGATGC CATTGATATC
    ATCTCAGACT TGGAATCATG GAATGATGAA CTTTCTCAGC AAATGAATGA TTTTGACACA
    GAGGACCTCA CACTAGCCGA GCAAAGGCTA CAGCATCACG CAGACAAAGC TCTGACAATG
    AACAACCTGA CATTTGATGT CATCCACCAA GGACAAGAGT TACTACAATA TGTGAATGAA
    GTTCAGAACT CAGGGGTTGA GCTACTCTGT GATAGAGATG TAGATATGGC TACTAGAGTT
    CAGGACCTGT TGGAATTCCT CCATGAGAAA CAACAGGAGT TGGATCTAGC AGCTGAGCAG
    CACCGAAAAC ATCTAGAACA ATGTGTGCAA CTACGACATT TGCAAGCTGA AGTCAAACAG
    GTACTCGGCT GGATACGGAA TGGAGAGTCT ATGTTAAATG CCGGGCTTAT CACTGCCAGT
    TCATTGCAAG AAGCAGAGCA ACTTCAAAGG GAACATGAGC AGTTCCAGCA TGCAATAGAA
    AAAACCCACC AGAGTGCTCT GCAGGTGCAG CAGAAGGCAG AGGCCATGCT TCAAGCAAAC
    CATTACGACA TGGATATGAT CCGGGACTGT GCTGAAAAAG TGGCCTCTCA TTGGCAACAG
    TTGATGCTTA AAATGGAAGA CCGTCTTAAG CTTGTCAATG CCTCTGTGGC CTTTTATAAA
    ACCTCAGAGC AGGTATGCAG TGTTCTAGAG AGCCTAGAGC AGGAGTACAA GCGGGAGGAA
    GATTGGTGTG GCGGAGCTGA TAAACTTGGG CCTAACTCAG AGACTGACCA TGTTACTCCT
    ATGATCAGCA AACACCTTGA ACAAAAGGAA GCCTTTCTTA AGGCCTGCAC TTTGGCTCGC
    AGAAATGCTG ATGTCTTTCT GAAATACTTG CACAGGAATA GTGTCAATAT GCCTGGAATG
    GTGACACATG TAAAGGCTCC TGAACAGCAA GTAAAAAATA TTTTAAATGA GCTGTTTCAG
    AGGGAAAATA GAGTTCTTCA TTATTGGACC TTGCGGAAAA GGAGACTAGA CCATTGCCAG
    CAGTATGTAG TATTTGAAAG AAGTGCCAAA CAGGCTCTGG AGTGGATCCA AGATAATGGA
    GAATTTTACA TTTCCACACA CACCTCCACT GGCTCCACAA TTCAGCATAC ACAAGAACTC
    TTAAAAGAGC ATGAGGAGTT TCAGATCACA GCAAAGCAAA CCAAAGAGAG GGTAAAGCTG
    TTGATACAGC TGGCTGATGG CTTTTGTGAA AAAGGGCATG CTCATGCTGC AGAAATTAAA
    AAATGTGTTA CAGCGGTGGA CAAGAGGTAC AGAGACTTCT CGCTGCGTAT GGAAAAGTAC
    AGAACCAGCT TAGAGAAGGC TTTGGGCATT TCTTCTGATT CCAAGTCGAG TAAAAGCCTT
    CAGTTGGATA TTATTCCAGC CACTGCCCCT GGTTCCGAAG TCAAGCTTCG TGATGCAACA
    CATGAACTCA ATGAGGAGAA AAGAAAATCT GCTCGTCGAA AAGAATTCAT CATGGCAGAA
    CTTATACAAA CTGAAAAAGC ATATGTTCGT GATCTTCGGG AATGCATGGA TACATATCTG
    TGGGAAATGA CAAGCGGAGT TGAAGAGATC CCACCAGGCA TTGTAAATAA AGAACACATT
    ATCTTTGGAA ACATGCAGGA AATATATGAA TTTCATAACA ACATATTCTT AAAGGAGCTT
    GAGAAGTATG AGCAGCTACC TGAAGATGTT GGACATTGTT TTGTCACATG GGCTGACAAA
    TTTCAGATGT ATGTGTCCTA CTGTAAGAAT AAGCCTGATT CAACACAACT TATCTTGGAA
    CAAGCAGGAA CATATTTTGA TGAAATTCAG CAGCGCCATG AGCTGGTCAA CTCCATCTCT
    TCTTATCTTA TCAAACCTGT GCAAAGGATA ACAAAATATC AGCTTCTTTT AAAAGAGCTG
    CTGACATGCT GCGAAGAAGG TAAAGGAGAG ATTAAAGATG GGCTGGAAGT GATGCTAAGT
    GTACCAAAAA GAGCCAACGA TGCAATGCAT CTCAGCATGC TTGAAGGGTT TGATGAGAAC
    ATTGAATCTC AGGGAGAACT TATTCTGCAA GAGTCTTTTC AAGTGTGGGA TCCTAAAACC
    TTAATAAGAA AGGGGCGTGA GAGACATCTC TTCTTGTTTG AAATGTCTTT GGTCTTCAGC
    AAAGAAGTGA AAGATTCAAG TGGAAGGAGC AAATATATTT ACAAATCAAA ATTATTTACC
    TCAGAGCTTG GAGTCACAGA ACATGTAGAA GGTGATCCTT GCAAATTTGC ACTTTGGGTA
    GGCAGAACGC CAACATCTGA TAACAAAATA GTCCTTAAGG GCTCAAGTAT TGAGAACAAG
    CAAGATTGGA TCAAGCATAT AAGAGAAGTG ATCCAAGACC GAACCATTCA CCTGAGAGGC
    GCATTAAAAG AGCCTATTCA TATACCTAAA ACAGCTGCTG CTACAAAACA GAAACCTGCC
    AAAAGAGATG GAGAAGACCT GGACAGTCAA GGAGATGGCA GCAGCCAACC TGATACAATA
    TCTATTGCTT CACGAACTTC ACAGAACACA TTAGACAGTG ATAAGCTTTC TGGTGGATGT
    GAGCTGACAG TAGTCATCCA TGACTTTACT GCCTGTAATA GCAATGAGCT GACAATTAAA
    CGAGGGCAGA CTGTGGAGGT TCTAGAGCGG CATCACGATC GACCTGACTG GTGTTTAGTT
    CGGACTACTG ACCGGTCACC TGCAGCAGAG GGCTTGGTGC CTAGTAGTTC ACTGTGTATT
    GCACATTCTA GAAGTAGCAT GGAAATGGAA GGCATATTTA ACCACAAAGA TTCCCTCTCT
    GTCTCCAGTA ATGATGCCAG TCCTCCTGCT TCAGTAATTT CCCTTCAGCC TCACATGATT
    GGTGCTCAAA GCTCTCCTGG CCCAAAGCGA CCTGGCAATA CTTTGAGAAA ATGGCTCACC
    AGCCCTGTCA GGAGACTTAG CAGTGGCAAA GCTGATGGAC ATGTGAAAAA ATTGGCTCAT
    AAACACAAAA AAAGCAGGGA GGTTCGTAAA AACACAGACT CAGGTTCCCA AAAGGACTCT
    GATGACAGTG CAGCAACACC TCAAGATGAA ACTATCGAGG AGAGAGTGCG CAATGAAGGA
    CTTAGCAGTG GTACGCTTTC TAAATCCTCC TCGTCTGGCA TGCAAAGCTG TGGAGAAGAA
    GAGGGCGAGG AAGGACCTGA TGCAGTTCCA TTGCCTCCAC CCATGGCTAT TCAGCAGCAC
    AGCCTTCTTC AGCCTGACTC ACAGGATGAC AAGACATCAT CTCGGTTGCT GGTGCGACCT
    ACTAGCTCAG AGACACCTAG TGCTGCAGAG CTTGTTAGTG CTATTGAAGA ACTTGTAAAG
    AGTAAAATGG CTTTGGAAGA CCGGCCAAGT TCACTTCAAG TAGACCAGGG AGACAGTAGT
    AGCCCATCAT TCAACCCTTC AGATACATCT CTGCTTTCCT CCTCTTCTCC AATTGATGAA
    ATGGAAGAAA AAAAATCAAG CTCTTTAAAA AGAAGGCACT ATGTATTACA AGAGCTAGTA
    GAAACTGAAC GCGATTATGT TCGGGACCTT GGCTATGTGG TTGAGGGTTA TATGGCACTT
    ATGAAGGAGG ATGGAGTTCC AGATGATATG AAAGGAAAAG ACAAGATTGT TTTTGGCAAC
    TTACACCAGA TCTACGAGTG GCACAGAGAC TTTTTTTTGG GTGAACTGGA AAGGTGCCTG
    GATGAGCCTG AAAGATTGGC ATCCCTATTT GTCAAGCATG AAAGAAGATT GCATATGTAC
    ATAGTTTACT GCCAAAATAA ACCAAAGTCT GAACATATTG TTTCAGAATA CATTGATACC
    TATTTTGAGG ACCTGAAACA GCGTCTGGGC CATCGACTTC AGCTTACAGA TCTGTTAATA
    AAACCCATAC AAAGAATAAT GAAATATCAG CTGCTATTAA AGGATTTTCT CAAGTACTCT
    AAGAAGGCTT GCATAGATGT AGCAGAACTT GAGAGAGCTG TGGAAGTCAT GTGTATCGTA
    CCGAAGCGGT GTAATGACAT GATGAATGTT GGTCGTTTGC AAGGATTTGA TGGCAAAATA
    GTTGCCCAAG GAAAGCTGCT ACTGCAAGAC ACATTCCTTG TGACTGAGCA GGACGCTGGT
    CTTCTGCCCC GATGCAAAGA AAGGCGGGTC TTCCTTTTCG AACAAATTGT CATCTTTAGT
    GAGCCCTTAG ATAAAAAGAA AGGATACTCT ACACCTGGAT TTCTTTTTAA AAATAATATC
    AAAGTGAGCT GCCTTAGTTT AGAGGAGTGT GTTGATAGTG ATCCATGCAA GTTTGCACTA
    TTGTCAAGGT CTGCTGACTC TACAGAGATG TTTGTTTTGC ATTCTTCATC GCCTGGAGTG
    AGACAGTTAT GGATTCATGA AATCAACCAA ATTTTGGAAA ACCAGCGCAA TTTTTTAAAT
    GCCTTGACAT CACCAATTGA ATACCACAGA AATCACACTG GTGGAAGCAG TAGTGGCAAC
    GTCACCTTTA CCAGCAGTCC CAGCAATTGT AGTGGGCTGG GTGGCTCCAG CCGAAGCAGG
    CCCTCTCGGA TTCCCCAGCC TGTTAGACAC CACTCTCCAG TCCTGGTCTC CTCTGCAGCT
    TCGGCACAGT CAGAGGCAGA CAAGATGTCA GGTATGTCAG CTCGCCGTGC ACCAGTTGTA
    CCTCTCAACC TCTCCTTAGA CAATAACCCC AGCCAGAAAG GTAGAGTAAC TGCTGCTCCA
    GAGACAGGCG GGCGAGATAA AGAATCCAAT TCAGTGCCAA AGATGAAAAT GCTTGATGGC
    CCCAGGAAAT CAGCTAGTGG CATTCCAACC TTAGCTGATG GTGCACAGAA AGAATTGCGT
    GGGAGCACAG AAGACAGTCG AATTAGAAGC ACTATGGGCA CTCTAACCTT AGGAAAGCCA
    AGAAATGGTT CTGCCTCACC CCTCAACTCT CCTGTCACAA TTAATTTTCC ATCGTCTTTT
    GGTAAGGAAT CAGGGCCACC AAGCAGTCCC CTTCAGAAAG GCTCTTTTTG GAGCTCAATT
    CCAGGATCTC CAGCCAGCCG ACCTGGCTCC TTCACTTTCC CTGGAGACAG TGACTCTCTC
    CAGCGACAAG TTCATCGGCA CTCCACACAC AGCAAGGATA CAGACCGCAT GAGCACCTGC
    TCCTCGACTA GTGAGCAGTC CGTTCACTCC ACTCAGAGTA ATGGGAGTGA AAGTAGCAGC
    AGTAGCAATA TCTCCACCAT GCTGGTTACG CACGATTATA CAGCGGTGAA GGAGGATGAG
    ATCAATGTCT ACCAGGGAGA GGTTGTACAA ATTCTGGCAA GTAACCAACA GAACATGTTT
    CTGGTGTTCA GAGCTGCCAC TGATGAGTGC CCTGCAGCTG AGGGCTGGAT TCCAAGCTAT
    GTTCTGGGTC ACACAAGTGC AATCATCACT GATATGACTG ATGCAACTAT GAAGAAGTCC
    TCTTCTTGGC ATACAGCCCT TAGAATAAGA AAGAAATCTG AGAAGAGGGA AAAAGATGGC
    AGCAGAGACA CAAAACTAGA GAATGGTTAT CGCAAATCAA GAGAAGGACT AGCTAATAAG
    GTATCAGTTA AGCTCCTGAA TCCAAACTAC ATTTATGATG CTCCTCCAGA GTTTATTGTA
    CCACTGAACG AGGTTACATG TGACACAGGA GAGACTGTGA TTATTCGATG CAAAGTTTGT
    GGTCGCCCCA AGGCATCCAT TACATGGAAA GGTCCCGACC ATAATACTTT GAGCAACGAT
    GGACACTACA GTATATCCTA CAGTGATCTT GGAGAAGCAA CTCTAAAAAT AGTTGGTGTG
    ACCACTGAGG ATGATGGTAT ATACACCTGC ATTGCAACAA ATGACTTGGG TTCAGTATCC
    GCTTCCTCCA ATCTTAGAGT GTTAGGTTCA TGGAGTGATG GACTGAGAGT AATCTGGAAA
    GAAAACTTCG ACATTGCTTA TAGTGAAGTG GCTGAACTTG GCAGGGGCAG GTTTTCTGTC
    GTGAAAAAAT GTGAACAAAG AGTTACAAGA CGCATAGTAG CCACCAAATT TGTTAACAAG
    AAGTTGATGA AAAGAGACCA GGTTACCCAT GAACTTGGAA TTCTGCAGAG CCTTCAGCAC
    CCACAACTGA TCAGCCTTCT TGACACTTTT GAGACACCAG CAAGCTATGT CTTAGTACTT
    GAGATAGCTG ATCAGGGATG TTTGTTAGAA TATGTGGTAC AGTGGGGAAA CCTTACAGAA
    GAAAAGATCA CCTCATATAT GAGAGAGGTG CTGGAGGCCC TGCAGTATCT TCATAATTGC
    AGAATAGCAC ATCTGGACCT AAAGCCTGAG AACATTCTTG TGGATCAGAG CCTAACCAAA
    CCAACTATAA AACTGTCAGA CTTTGGCGAT GCTGTTCAGC TTAATACCAC TTACTACATT
    CACCAATTGT TGGGCAGTGC AGAGTTTGCC GCTCCAGAGA TCATCCTTGG GAATCCTGTC
    TCCCTTACCT CTGATATATG GAGTATTGGA GTTCTTACTT ATGTCCTTCT CAGTGGCGTC
    TCTCCTTTCC TTGATGAAAG TGTTGAAGAG ACATGCCTGA ACATTTGCCG ACTAGACTTT
    AGTTTTCCCG ATGACTACTT TAAGGGAGTA AGTCAGAAGG CCAAAGAATT TGTATGTTTC
    TTACTGCAGG GTGACCAGAC AAAGCGCCCT TCTGCTGCTG TATGTCTAAA GGAACAGTGG
    TTGCAGCCAG GCCAGCACAA ACGTAGTGGC ACATTAGACA TGTCAAGACT GACCTCGTTT
    ATTGAACGCC GTAAACACCA GAATGATGTT CGACCCATTG GTAGCATTAA AAACTTCCTT
    CAGAGTCGCC TTTTACCCAG AATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001123393.1
    CDS176..9322
    Translation

    Target ORF information:

    RefSeq Version NM_001129921.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001129921.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGCAGACTC ATCGCTGGAA AGATGACGGA GAAACAACAT TAGGTTTTCG TAGAAATGAT 
    GAATTGAAAG CAATGGAGGT GTTGCCAATT TTAAAAGAAA AAGTGGCATA TCTATCAGGT
    GGCAGAGATA AACGAGGTGG TCCTGTATTA ACATTTCCAG CACGCAGCAA CCATGATCGA
    ATACGACAAG AGGATCTCCG AAGACTGATT TCATATCTTG CTTGTATTCC TAGTGAGGAG
    GTGTGCAAGC GAGGCTTCAC AGTGATTGTA GATATGCGTG GTTCCAAGTG GGATTCTATT
    AAACCCTTGC TGAAAATTTT ACAAGAATCC TTTCCATGCT GTATTCATGT TGCACTAATT
    ATCAAACCAG ATAACTTCTG GCAGAAGCAA AGAACTAATT TTGGAAGTTC TAAGTTTGAA
    TTTGAGACAA GTATGGTGTC TTTAGAAGGC CTTACCAAAG TAGTTGACCC TTCCCAGCTA
    ACTCCAGAAT TTGAGGGTTG CTTGGAATAC AACCATGAAG AGTGGATAGA GATCAGAGTA
    GCATTTGAGG ATTTTATTAA TAATGCAACT CATATGCTGT CTCGATTAGA GGAATTACAG
    GAGATGCTTG CAATTAAAGA GTTGCCTCAG GATTTAGAGG GGGCACGCAA TATGATTGAA
    GAACATTCTC AAATGAAAAA AAGGGTTATA AAGGCTCCTA TTGAAGATCT TGATTTAGAA
    GGGCAAAAGC TTTTACAAAC AATACAAAGC AGTGATAGCT TTCCTAAGAA GAACTCTAGT
    TCTGGAAATG CAGACCTTCA AAATCTTCTT CCCAAAGTTT CCACAATGTT AGACAGGCTT
    CATTCAACAC GACAACATCT TCATCAGATG TGGCACATGC GAAAAGTAAA ACTGGACCAG
    TGCTTTCAGC TAAGGTTGTT TGAGCAGGAT GCTGAGAAGA TGTTTGACTG GATTACACAC
    AACAAAGGTC TGTTTCTGAC AAGTTATACT GACATTGGCA CCCGTAATAA TCATGCTATG
    GAACTTCAGA CACAGCACAA TCATTTTGCT ATGAACTGCA TGAATGTCTA TGTAAACATT
    AGCCGAATAA TGTCTGTGGC AAATCGACTA GTAGAATCTG GACACTATGC CTCACAGCAA
    ATTAAGCAGA TAGCTAACCA GTTGGAGCAA GAGTGGAAGG CTTTTGCCGC AGCCCTTGAT
    GAACGTAGTA CGTTGCTTGA CATGTCTGCA ATGTTCCATC AAAAAGCGGA GCAGTACATG
    GGCAATGTGG ATTCATGGTG CAAAGCTTGT GGGGAAGTGG ATCTTCCATC AGAGCTGCAG
    GATCTAGAAG ATGCCATTCA TCACCATCAG GGAATATATG AGCATATCAC TTTAGCATAT
    TCAGAGGTTA GCCAGGATGG TAAATCTTTA TTAGACAAAC TCCAGAGACC ATTGACTCCT
    GGTAGTTCTG ACTCACTGAC AGCTTCTGCC AACTATTCAA AGGCTGTGCA TCACGTCTTA
    GATGCAATAC ATGAAGTCTT GCACCACCAG CGTCAGTTGG AAAACATCTG GCAACATCGC
    AAAGTTCGTC TGCATCAACG CCTACAGCTC TGTGTTTTTC AGCAAGATGT TCAGCAGGTC
    TTGGATTGGA TAGAGAACCA TGGTGAAGCC TTTCTCAGCA AACACACTGG GGTAGGCAAG
    TCTCTGCACA GGGCCAGAGC CTTGCAAAAG CGACATGAAG ACTTTGAAGA AGTCGCTCAG
    AATACATACA CCAATGCTGA TAAGCTGCTG GAAGCTGCAG AACAGTTGGC CCAGACAGGA
    GAGTGCGATC CAGAGGAAAT ATACCAGGCT GCTCATCAGC TTGAGGATCG GATTCAGGAT
    TTTGTAAGAC GTGTAGAACA AAGAAAAGTT CTCTTGGACA TGTCTGTATC ATTCCACTCG
    CATGTTAAAG AGCTATGGAC ATGGCTGGAG GACCTACAAA AGGAACTGCT GGATGATGTC
    TATGCGGAGT CAGTAGAAGC TGTACAGGAC TTGATCAAAC GGTTTGGACA ACAGCAGCAG
    ACAACCCTCC AGTTTACAGT AAATGTCATC AAAGAAGGGG AGGATCTAAT TCAGCAACTA
    AGAGATTCTG CTATATCCAG CAACAAAACT CCTCACAACA GCTCAATCAA TCACATTGAG
    ACTGTCCTTC AACAACTTGA TGAGGCCCAA TCACAGATGG AGGAGCTTTT CCAAGAGAGA
    AAAATCAAAC TTGAGCTATT CTTGCAGCTT CGCATCTTTG AGAGAGATGC CATTGATATC
    ATCTCAGACT TGGAATCATG GAATGATGAA CTTTCTCAGC AAATGAATGA TTTTGACACA
    GAGGACCTCA CACTAGCCGA GCAAAGGCTA CAGCATCACG CAGACAAAGC TCTGACAATG
    AACAACCTGA CATTTGATGT CATCCACCAA GGACAAGAGT TACTACAATA TGTGAATGAA
    GTTCAGAACT CAGGGGTTGA GCTACTCTGT GATAGAGATG TAGATATGGC TACTAGAGTT
    CAGGACCTGT TGGAATTCCT CCATGAGAAA CAACAGGAGT TGGATCTAGC AGCTGAGCAG
    CACCGAAAAC ATCTAGAACA ATGTGTGCAA CTACGACATT TGCAAGCTGA AGTCAAACAG
    GTACTCGGCT GGATACGGAA TGGAGAGTCT ATGTTAAATG CCGGGCTTAT CACTGCCAGT
    TCATTGCAAG AAGCAGAGCA ACTTCAAAGG GAACATGAGC AGTTCCAGCA TGCAATAGAA
    AAAACCCACC AGAGTGCTCT GCAGGTGCAG CAGAAGGCAG AGGCCATGCT TCAAGCAAAC
    CATTACGACA TGGATATGAT CCGGGACTGT GCTGAAAAAG TGGCCTCTCA TTGGCAACAG
    TTGATGCTTA AAATGGAAGA CCGTCTTAAG CTTGTCAATG CCTCTGTGGC CTTTTATAAA
    ACCTCAGAGC AGGTATGCAG TGTTCTAGAG AGCCTAGAGC AGGAGTACAA GCGGGAGGAA
    GATTGGTGTG GCGGAGCTGA TAAACTTGGG CCTAACTCAG AGACTGACCA TGTTACTCCT
    ATGATCAGCA AACACCTTGA ACAAAAGGAA GCCTTTCTTA AGGCCTGCAC TTTGGCTCGC
    AGAAATGCTG ATGTCTTTCT GAAATACTTG CACAGGAATA GTGTCAATAT GCCTGGAATG
    GTGACACATG TAAAGGCTCC TGAACAGCAA GTAAAAAATA TTTTAAATGA GCTGTTTCAG
    AGGGAAAATA GAGTTCTTCA TTATTGGACC TTGCGGAAAA GGAGACTAGA CCATTGCCAG
    CAGTATGTAG TATTTGAAAG AAGTGCCAAA CAGGCTCTGG AGTGGATCCA AGATAATGGA
    GAATTTTACA TTTCCACACA CACCTCCACT GGCTCCACAA TTCAGCATAC ACAAGAACTC
    TTAAAAGAGC ATGAGGAGTT TCAGATCACA GCAAAGCAAA CCAAAGAGAG GGTAAAGCTG
    TTGATACAGC TGGCTGATGG CTTTTGTGAA AAAGGGCATG CTCATGCTGC AGAAATTAAA
    AAATGTGTTA CAGCGGTGGA CAAGAGGTAC AGAGACTTCT CGCTGCGTAT GGAAAAGTAC
    AGAACCAGCT TAGAGAAGGC TTTGGGCATT TCTTCTGATT CCAAGTCGAG TAAAAGCCTT
    CAGTTGGATA TTATTCCAGC CACTGCCCCT GGTTCCGAAG TCAAGCTTCG TGATGCAACA
    CATGAACTCA ATGAGGAGAA AAGAAAATCT GCTCGTCGAA AAGAATTCAT CATGGCAGAA
    CTTATACAAA CTGAAAAAGC ATATGTTCGT GATCTTCGGG AATGCATGGA TACATATCTG
    TGGGAAATGA CAAGCGGAGT TGAAGAGATC CCACCAGGCA TTGTAAATAA AGAACACATT
    ATCTTTGGAA ACATGCAGGA AATATATGAA TTTCATAACA ACATATTCTT AAAGGAGCTT
    GAGAAGTATG AGCAGCTACC TGAAGATGTT GGACATTGTT TTGTCACATG GGCTGACAAA
    TTTCAGATGT ATGTGTCCTA CTGTAAGAAT AAGCCTGATT CAACACAACT TATCTTGGAA
    CAAGCAGGAA CATATTTTGA TGAAATTCAG CAGCGCCATG AGCTGGTCAA CTCCATCTCT
    TCTTATCTTA TCAAACCTGT GCAAAGGATA ACAAAATATC AGCTTCTTTT AAAAGAGCTG
    CTGACATGCT GCGAAGAAGG TAAAGGAGAG ATTAAAGATG GGCTGGAAGT GATGCTAAGT
    GTACCAAAAA GAGCCAACGA TGCAATGCAT CTCAGCATGC TTGAAGGGTT TGATGAGAAC
    ATTGAATCTC AGGGAGAACT TATTCTGCAA GAGTCTTTTC AAGTGTGGGA TCCTAAAACC
    TTAATAAGAA AGGGGCGTGA GAGACATCTC TTCTTGTTTG AAATGTCTTT GGTCTTCAGC
    AAAGAAGTGA AAGATTCAAG TGGAAGGAGC AAATATATTT ACAAATCAAA ATTATTTACC
    TCAGAGCTTG GAGTCACAGA ACATGTAGAA GGTGATCCTT GCAAATTTGC ACTTTGGGTA
    GGCAGAACGC CAACATCTGA TAACAAAATA GTCCTTAAGG GCTCAAGTAT TGAGAACAAG
    CAAGATTGGA TCAAGCATAT AAGAGAAGTG ATCCAAGACC GAACCATTCA CCTGAGAGGC
    GCATTAAAAG AGCCTATTCA TATACCTAAA ACAGCTGCTG CTACAAAACA GAAACCTGCC
    AAAAGAGATG GAGAAGACCT GGACAGTCAA GGAGATGGCA GCAGCCAACC TGATACAATA
    TCTATTGCTT CACGAACTTC ACAGAACACA TTAGACAGTG ATAAGCTTTC TGGTGGATGT
    GAGCTGACAG TAGTCATCCA TGACTTTACT GCCTGTAATA GCAATGAGCT GACAATTAAA
    CGAGGGCAGA CTGTGGAGGT TCTAGAGCGG CATCACGATC GACCTGACTG GTGTTTAGTT
    CGGACTACTG ACCGGTCACC TGCAGCAGAG GGCTTGGTGC CTAGTAGTTC ACTGTGTATT
    GCACATTCTA GAAGTAGCAT GGAAATGGAA GGCATATTTA ACCACAAAGA TTCCCTCTCT
    GTCTCCAGTA ATGATGCCAG TCCTCCTGCT TCAGTAATTT CCCTTCAGCC TCACATGATT
    GGTGCTCAAA GCTCTCCTGG CCCAAAGCGA CCTGGCAATA CTTTGAGAAA ATGGCTCACC
    AGCCCTGTCA GGAGACTTAG CAGTGGCAAA GCTGATGGAC ATGTGAAAAA ATTGGCTCAT
    AAACACAAAA AAAGCAGGGA GGTTCGTAAA AACACAGACT CAGGTTCCCA AAAGGACTCT
    GATGACAGTG CAGCAACACC TCAAGATGAA ACTATCGAGG AGAGAGTGCG CAATGAAGGA
    CTTAGCAGTG GTACGCTTTC TAAATCCTCC TCGTCTGGCA TGCAAAGCTG TGGAGAAGAA
    GAGGGCGAGG AAGGACCTGA TGCAGTTCCA TTGCCTCCAC CCATGGCTAT TCAGCAGCAC
    AGCCTTCTTC AGCCTGACTC ACAGGATGAC AAGACATCAT CTCGGTTGCT GGTGCGACCT
    ACTAGCTCAG AGACACCTAG TGCTGCAGAG CTTGTTAGTG CTATTGAAGA ACTTGTAAAG
    AGTAAAATGG CTTTGGAAGA CCGGCCAAGT TCACTTCAAG TAGACCAGGG AGACAGTAGT
    AGCCCATCAT TCAACCCTTC AGATACATCT CTGCTTTCCT CCTCTTCTCC AATTGATGAA
    ATGGAAGAAA AAAAATCAAG CTCTTTAAAA AGAAGGCACT ATGTATTACA AGAGCTAGTA
    GAAACTGAAC GCGATTATGT TCGGGACCTT GGCTATGTGG TTGAGGGTTA TATGGCACTT
    ATGAAGGAGG ATGGAGTTCC AGATGATATG AAAGGAAAAG ACAAGATTGT TTTTGGCAAC
    TTACACCAGA TCTACGAGTG GCACAGAGAC TTTTTTTTGG GTGAACTGGA AAGGTGCCTG
    GATGAGCCTG AAAGATTGGC ATCCCTATTT GTCAAGCATG AAAGAAGATT GCATATGTAC
    ATAGTTTACT GCCAAAATAA ACCAAAGTCT GAACATATTG TTTCAGAATA CATTGATACC
    TATTTTGAGG ACCTGAAACA GCGTCTGGGC CATCGACTTC AGCTTACAGA TCTGTTAATA
    AAACCCATAC AAAGAATAAT GAAATATCAG CTGCTATTAA AGGATTTTCT CAAGTACTCT
    AAGAAGGCTT GCATAGATGT AGCAGAACTT GAGAGAGCTG TGGAAGTCAT GTGTATCGTA
    CCGAAGCGGT GTAATGACAT GATGAATGTT GGTCGTTTGC AAGGATTTGA TGGCAAAATA
    GTTGCCCAAG GAAAGCTGCT ACTGCAAGAC ACATTCCTTG TGACTGAGCA GGACGCTGGT
    CTTCTGCCCC GATGCAAAGA AAGGCGGGTC TTCCTTTTCG AACAAATTGT CATCTTTAGT
    GAGCCCTTAG ATAAAAAGAA AGGATACTCT ACACCTGGAT TTCTTTTTAA AAATAATATC
    AAAGTGAGCT GCCTTAGTTT AGAGGAGTGT GTTGATAGTG ATCCATGCAA GTTTGCACTA
    TTGTCAAGGT CTGCTGACTC TACAGAGATG TTTGTTTTGC ATTCTTCATC GCCTGGAGTG
    AGACAGTTAT GGATTCATGA AATCAACCAA ATTTTGGAAA ACCAGCGCAA TTTTTTAAAT
    GCCTTGACAT CACCAATTGA ATACCACAGA AATCACACTG GTGGAAGCAG TAGTGGCAAC
    GTCACCTTTA CCAGCAGTCC CAGCAATTGT AGTGGGCTGG GTGGCTCCAG CCGAAGCAGG
    CCCTCTCGGA TTCCCCAGCC TGTTAGACAC CACTCTCCAG TCCTGGTCTC CTCTGCAGCT
    TCGGCACAGT CAGAGGCAGA CAAGATGTCA GGTATGTCAG CTCGCCGTGC ACCAGTTGTA
    CCTCTCAACC TCTCCTTAGA CAATAACCCC AGCCAGAAAG GTAGAGTAAC TGCTGCTCCA
    GAGACAGGCG GGCGAGATAA AGAATCCAAT TCAGTGCCAA AGATGAAAAT GCTTGATGGC
    CCCAGGAAAT CAGCTAGTGG CATTCCAACC TTAGCTGATG GTGCACAGAA AGAATTGCGT
    GGGAGCACAG AAGACAGTCG AATTAGAAGC ACTATGGGCA CTCTAACCTT AGGAAAGCCA
    AGAAATGGTT CTGCCTCACC CCTCAACTCT CCTGTCACAA TTAATTTTCC ATCGTCTTTT
    GGTAAGGAAT CAGGGCCACC AAGCAGTCCC CTTCAGAAAG GCTCTTTTTG GAGCTCAATT
    CCAGGATCTC CAGCCAGCCG ACCTGGCTCC TTCACTTTCC CTGGAGACAG TGACTCTCTC
    CAGCGACAAG TTCATCGGCA CTCCACACAC AGCAAGGATA CAGACCGCAT GAGCACCTGC
    TCCTCGACTA GTGAGCAGTC CGTTCACTCC ACTCAGAGTA ATGGGAGTGA AAGTAGCAGC
    AGTAGCAATA TCTCCACCAT GCTGGTTACG CACGATTATA CAGCGGTGAA GGAGGATGAG
    ATCAATGTCT ACCAGGGAGA GGTTGTACAA ATTCTGGCAA GTAACCAACA GAACATGTTT
    CTGGTGTTCA GAGCTGCCAC TGATGAGTGC CCTGCAGCTG AGGGCTGGAT TCCAAGCTAT
    GTTCTGGGTC ACACAAGTGC AATCATCACT GATATGACTG ATGCAACTAT GAAGAAGTCC
    TCTTCTTGGC ATACAGCCCT TAGAATAAGA AAGAAATCTG AGAAGAGGGA AAAAGATGGC
    AGCAGAGACA CAAAACTAGA GAATGGTTAT CGCAAATCAA GAGAAGGACT AGCTAATAAG
    GTATCAGTTA AGCTCCTGAA TCCAAACTAC ATTTATGATG CTCCTCCAGA GTTTATTGTA
    CCACTGAACG AGGTTACATG TGACACAGGA GAGACTGTGA TTATTCGATG CAAAGTTTGT
    GGTCGCCCCA AGGCATCCAT TACATGGAAA GGTCCCGACC ATAATACTTT GAGCAACGAT
    GGACACTACA GTATATCCTA CAGTGATCTT GGAGAAGCAA CTCTAAAAAT AGTTGGTGTG
    ACCACTGAGG ATGATGGTAT ATACACCTGC ATTGCAACAA ATGACTTGGG TTCAGTATCC
    GCTTCCTCCA ATCTTAGAGT GTTAGGTTCA TGGAGTGATG GACTGAGAGT AATCTGGAAA
    GAAAACTTCG ACATTGCTTA TAGTGAAGTG GCTGAACTTG GCAGGGGCAG GTTTTCTGTC
    GTGAAAAAAT GTGAACAAAG AGTTACAAGA CGCATAGTAG CCACCAAATT TGTTAACAAG
    AAGTTGATGA AAAGAGACCA GGTTACCCAT GAACTTGGAA TTCTGCAGAG CCTTCAGCAC
    CCACAACTGA TCAGCCTTCT TGACACTTTT GAGACACCAG CAAGCTATGT CTTAGTACTT
    GAGATAGCTG ATCAGGGATG TTTGTTAGAA TATGTGGTAC AGTGGGGAAA CCTTACAGAA
    GAAAAGATCA CCTCATATAT GAGAGAGGTG CTGGAGGCCC TGCAGTATCT TCATAATTGC
    AGAATAGCAC ATCTGGACCT AAAGCCTGAG AACATTCTTG TGGATCAGAG CCTAACCAAA
    CCAACTATAA AACTGTCAGA CTTTGGCGAT GCTGTTCAGC TTAATACCAC TTACTACATT
    CACCAATTGT TGGGCAGTGC AGAGTTTGCC GCTCCAGAGA TCATCCTTGG GAATCCTGTC
    TCCCTTACCT CTGATATATG GAGTATTGGA GTTCTTACTT ATGTCCTTCT CAGTGGCGTC
    TCTCCTTTCC TTGATGAAAG TGTTGAAGAG ACATGCCTGA ACATTTGCCG ACTAGACTTT
    AGTTTTCCCG ATGACTACTT TAAGGGAGTA AGTCAGAAGG CCAAAGAATT TGTATGTTTC
    TTACTGCAGG GTGACCAGAC AAAGCGCCCT TCTGCTGCTG TATGTCTAAA GGAACAGTGG
    TTGCAGCCAG GCCAGCACAA ACGTAGTGGC ACATTAGACA TGTCAAGACT GACCTCGTTT
    ATTGAACGCC GTAAACACCA GAATGATGTT CGACCCATTG GTAGCATTAA AAACTTCCTT
    CAGAGTCGCC TTTTACCCAG AATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32253
    Clone ID Related Accession (Same CDS sequence) XM_012964353.2 , XM_012964353.3
    Accession Version XM_012964353.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9258bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product triple functional domain protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964353.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGAGCACAT CCAGCACCGC AAGCTCCGGT AGCGCAAATA CCGGTACCGG CAATGCCGTT 
    GGAAGCAGCG GAGGCAACAC CGGGGAGGGG GCGGAGGAGA CGGCCAAGGA CTCTGCAGAC
    ATAGCTGCCT TTTACAGATC CGGTTTTCGT AGAAATGATG AATTGAAAGC AATGGAGGTG
    TTGCCAATTT TAAAAGAAAA AGTGGCATAT CTATCAGGTG GCAGAGATAA ACGAGGTGGT
    CCTGTATTAA CATTTCCAGC ACGCAGCAAC CATGATCGAA TACGACAAGA GGATCTCCGA
    AGACTGATTT CATATCTTGC TTGTATTCCT AGTGAGGAGG TGTGCAAGCG AGGCTTCACA
    GTGATTGTAG ATATGCGTGG TTCCAAGTGG GATTCTATTA AACCCTTGCT GAAAATTTTA
    CAAGAATCCT TTCCATGCTG TATTCATGTT GCACTAATTA TCAAACCAGA TAACTTCTGG
    CAGAAGCAAA GAACTAATTT TGGAAGTTCT AAGTTTGAAT TTGAGACAAG TATGGTGTCT
    TTAGAAGGCC TTACCAAAGT AGTTGACCCT TCCCAGCTAA CTCCAGAATT TGAGGGTTGC
    TTGGAATACA ACCATGAAGA GTGGATAGAG ATCAGAGTAG CATTTGAGGA TTTTATTAAT
    AATGCAACTC ATATGCTGTC TCGATTAGAG GAATTACAGG AGATGCTTGC AATTAAAGAG
    TTGCCTCAGG ATTTAGAGGG GGCACGCAAT ATGATTGAAG AACATTCTCA AATGAAAAAA
    AGGGTTATAA AGGCTCCTAT TGAAGATCTT GATTTAGAAG GGCAAAAGCT TTTACAAACA
    ATACAAAGCA GTGATAGCTT TCCTAAGAAG AACTCTAGTT CTGGAAATGC AGACCTTCAA
    AATCTTCTTC CCAAAGTTTC CACAATGTTA GACAGGCTTC ATTCAACACG ACAACATCTT
    CATCAGATGT GGCACATGCG AAAAGTAAAA CTGGACCAGT GCTTTCAGCT AAGGTTGTTT
    GAGCAGGATG CTGAGAAGAT GTTTGACTGG ATTACACACA ACAAAGGTCT GTTTCTGACA
    AGTTATACTG ACATTGGCAC CCGTAATAAT CATGCTATGG AACTTCAGAC ACAGCACAAT
    CATTTTGCTA TGAACTGCAT GAATGTCTAT GTAAACATTA GCCGAATAAT GTCTGTGGCA
    AATCGACTAG TAGAATCTGG ACACTATGCC TCACAGCAAA TTAAGCAGAT AGCTAACCAG
    TTGGAGCAAG AGTGGAAGGC TTTTGCCGCA GCCCTTGATG AACGTAGTAC GTTGCTTGAC
    ATGTCTGCAA TGTTCCATCA AAAAGCGGAG CAGTACATGG GCAATGTGGA TTCATGGTGC
    AAAGCTTGTG GGGAAGTGGA TCTTCCATCA GAGCTGCAGG ATCTAGAAGA TGCCATTCAT
    CACCATCAGG GAATATATGA GCATATCACT TTAGCATATT CAGAGGTTAG CCAGGATGGT
    AAATCTTTAT TAGACAAACT CCAGAGACCA TTGACTCCTG GTAGTTCTGA CTCACTGACA
    GCTTCTGCCA ACTATTCAAA GGCTGTGCAT CACGTCTTAG ATGCAATACA TGAAGTCTTG
    CACCACCAGC GTCAGTTGGA AAACATCTGG CAACATCGCA AAGTTCGTCT GCATCAACGC
    CTACAGCTCT GTGTTTTTCA GCAAGATGTT CAGCAGGTCT TGGATTGGAT AGAGAACCAT
    GGTGAAGCCT TTCTCAGCAA ACACACTGGG GTAGGCAAGT CTCTGCACAG GGCCAGAGCC
    TTGCAAAAGC GACATGAAGA CTTTGAAGAA GTCGCTCAGA ATACATACAC CAATGCTGAT
    AAGCTGCTGG AAGCTGCAGA ACAGTTGGCC CAGACAGGAG AGTGCGATCC AGAGGAAATA
    TACCAGGCTG CTCATCAGCT TGAGGATCGG ATTCAGGATT TTGTAAGACG TGTAGAACAA
    AGAAAAGTTC TCTTGGACAT GTCTGTATCA TTCCACTCGC ATGTTAAAGA GCTATGGACA
    TGGCTGGAGG ACCTACAAAA GGAACTGCTG GATGATGTCT ATGCGGAGTC AGTAGAAGCT
    GTACAGGACT TGATCAAACG GTTTGGACAA CAGCAGCAGA CAACCCTCCA GTTTACAGTA
    AATGTCATCA AAGAAGGGGA GGATCTAATT CAGCAACTAA GAGATTCTGC TATATCCAGC
    AACAAAACTC CTCACAACAG CTCAATCAAT CACATTGAGA CTGTCCTTCA ACAACTTGAT
    GAGGCCCAAT CACAGATGGA GGAGCTTTTC CAAGAGAGAA AAATCAAACT TGAGCTATTC
    TTGCAGCTTC GCATCTTTGA GAGAGATGCC ATTGATATCA TCTCAGACTT GGAATCATGG
    AATGATGAAC TTTCTCAGCA AATGAATGAT TTTGACACAG AGGACCTCAC ACTAGCCGAG
    CAAAGGCTAC AGCATCACGC AGACAAAGCT CTGACAATGA ACAACCTGAC ATTTGATGTC
    ATCCACCAAG GACAAGAGTT ACTACAATAT GTGAATGAAG TTCAGAACTC AGGGGTTGAG
    CTACTCTGTG ATAGAGATGT AGATATGGCT ACTAGAGTTC AGGACCTGTT GGAATTCCTC
    CATGAGAAAC AACAGGAGTT GGATCTAGCA GCTGAGCAGC ACCGAAAACA TCTAGAACAA
    TGTGTGCAAC TACGACATTT GCAAGCTGAA GTCAAACAGG TACTCGGCTG GATACGGAAT
    GGAGAGTCTA TGTTAAATGC CGGGCTTATC ACTGCCAGTT CATTGCAAGA AGCAGAGCAA
    CTTCAAAGGG AACATGAGCA GTTCCAGCAT GCAATAGAAA AAACCCACCA GAGTGCTCTG
    CAGGTGCAGC AGAAGGCAGA GGCCATGCTT CAAGCAAACC ATTACGACAT GGATATGATC
    CGGGACTGTG CTGAAAAAGT GGCCTCTCAT TGGCAACAGT TGATGCTTAA AATGGAAGAC
    CGTCTTAAGC TTGTCAATGC CTCTGTGGCC TTTTATAAAA CCTCAGAGCA GGTATGCAGT
    GTTCTAGAGA GCCTAGAGCA GGAGTACAAG CGGGAGGAAG ATTGGTGTGG CGGAGCTGAT
    AAACTTGGGC CTAACTCAGA GACTGACCAT GTTACTCCTA TGATCAGCAA ACACCTTGAA
    CAAAAGGAAG CCTTTCTTAA GGCCTGCACT TTGGCTCGCA GAAATGCTGA TGTCTTTCTG
    AAATACTTGC ACAGGAATAG TGTCAATATG CCTGGAATGG TGACACATGT AAAGGCTCCT
    GAACAGCAAG TAAAAAATAT TTTAAATGAG CTGTTTCAGA GGGAAAATAG AGTTCTTCAT
    TATTGGACCT TGCGGAAAAG GAGACTAGAC CATTGCCAGC AGTATGTAGT ATTTGAAAGA
    AGTGCCAAAC AGGCTCTGGA GTGGATCCAA GATAATGGAG AATTTTACAT TTCCACACAC
    ACCTCCACTG GCTCCACAAT TCAGCATACA CAAGAACTCT TAAAAGAGCA TGAGGAGTTT
    CAGATCACAG CAAAGCAAAC CAAAGAGAGG GTAAAGCTGT TGATACAGCT GGCTGATGGC
    TTTTGTGAAA AAGGGCATGC TCATGCTGCA GAAATTAAAA AATGTGTTAC AGCGGTGGAC
    AAGAGGTACA GAGACTTCTC GCTGCGTATG GAAAAGTACA GAACCAGCTT AGAGAAGGCT
    TTGGGCATTT CTTCTGATTC CAAGTCGAGT AAAAGCCTTC AGTTGGATAT TATTCCAGCC
    ACTGCCCCTG GTTCCGAAGT CAAGCTTCGT GATGCAACAC ATGAACTCAA TGAGGAGAAA
    AGAAAATCTG CTCGTCGAAA AGAATTCATC ATGGCAGAAC TTATACAAAC TGAAAAAGCA
    TATGTTCGTG ATCTTCGGGA ATGCATGGAT ACATATCTGT GGGAAATGAC AAGCGGAGTT
    GAAGAGATCC CACCAGGCAT TGTAAATAAA GAACACATTA TCTTTGGAAA CATGCAGGAA
    ATATATGAAT TTCATAACAA CATATTCTTA AAGGAGCTTG AGAAGTATGA GCAGCTACCT
    GAAGATGTTG GACATTGTTT TGTCACATGG GCTGACAAAT TTCAGATGTA TGTGTCCTAC
    TGTAAGAATA AGCCTGATTC AACACAACTT ATCTTGGAAC AAGCAGGAAC ATATTTTGAT
    GAAATTCAGC AGCGCCATGA GCTGGTCAAC TCCATCTCTT CTTATCTTAT CAAACCTGTG
    CAAAGGATAA CAAAATATCA GCTTCTTTTA AAAGAGCTGC TGACATGCTG CGAAGAAGGT
    AAAGGAGAGA TTAAAGATGG GCTGGAAGTG ATGCTAAGTG TACCAAAAAG AGCCAACGAT
    GCAATGCATC TCAGCATGCT TGAAGGGTTT GATGAGAACA TTGAATCTCA GGGAGAACTT
    ATTCTGCAAG AGTCTTTTCA AGTGTGGGAT CCTAAAACCT TAATAAGAAA GGGGCGTGAG
    AGACATCTCT TCTTGTTTGA AATGTCTTTG GTCTTCAGCA AAGAAGTGAA AGATTCAAGT
    GGAAGGAGCA AATATATTTA CAAATCAAAA TTATTTACCT CAGAGCTTGG AGTCACAGAA
    CATGTAGAAG GTGATCCTTG CAAATTTGCA CTTTGGGTAG GCAGAACGCC AACATCTGAT
    AACAAAATAG TCCTTAAGGG CTCAAGTATT GAGAACAAGC AAGATTGGAT CAAGCATATA
    AGAGAAGTGA TCCAAGACCG AACCATTCAC CTGAGAGGCG CATTAAAAGA GCCTATTCAT
    ATACCTAAAA CAGCTGCTGC TACAAAACAG AAACCTGCCA AAAGAGATGG AGAAGACCTG
    GACAGTCAAG GAGATGGCAG CAGCCAACCT GATACAATAT CTATTGCTTC ACGAACTTCA
    CAGAACACAT TAGACAGTGA TAAGCTTTCT GGTGGATGTG AGCTGACAGT AGTCATCCAT
    GACTTTACTG CCTGTAATAG CAATGAGCTG ACAATTAAAC GAGGGCAGAC TGTGGAGGTT
    CTAGAGCGGC ATCACGATCG ACCTGACTGG TGTTTAGTTC GGACTACTGA CCGGTCACCT
    GCAGCAGAGG GCTTGGTGCC TAGTAGTTCA CTGTGTATTG CACATTCTAG AAGTAGCATG
    GAAATGGAAG GCATATTTAA CCACAAAGAT TCCCTCTCTG TCTCCAGTAA TGATGCCAGT
    CCTCCTGCTT CAGTAATTTC CCTTCAGCCT CACATGATTG GTGCTCAAAG CTCTCCTGGC
    CCAAAGCGAC CTGGCAATAC TTTGAGAAAA TGGCTCACCA GCCCTGTCAG GAGACTTAGC
    AGTGGCAAAG CTGATGGACA TGTGAAAAAA TTGGCTCATA AACACAAAAA AAGCAGGGAG
    GTTCGTAAAA ACACAGACTC AGGTTCCCAA AAGGACTCTG ATGACAGTGC AGCAACACCT
    CAAGATGAAA CTATCGAGGA GAGAGTGCGC AATGAAGGAC TTAGCAGTGG TACGCTTTCT
    AAATCCTCCT CGTCTGGCAT GCAAAGCTGT GGAGAAGAAG AGGGCGAGGA AGGACCTGAT
    GCAGTTCCAT TGCCTCCACC CATGGCTATT CAGCAGCACA GCCTTCTTCA GCCTGACTCA
    CAGGATGACA AGACATCATC TCGGTTGCTG GTGCGACCTA CTAGCTCAGA GACACCTAGT
    GCTGCAGAGC TTGTTAGTGC TATTGAAGAA CTTGTAAAGA GTAAAATGGC TTTGGAAGAC
    CGGCCAAGTT CACTTCAAGT AGACCAGGGA GACAGTAGTA GCCCATCATT CAACCCTTCA
    GATACATCTC TGCTTTCCTC CTCTTCTCCA ATTGATGAAA TGGAAGAAAA AAAATCAAGC
    TCTTTAAAAA GAAGGCACTA TGTATTACAA GAGCTAGTAG AAACTGAACG CGATTATGTT
    CGGGACCTTG GCTATGTGGT TGAGGGTTAT ATGGCACTTA TGAAGGAGGA TGGAGTTCCA
    GATGATATGA AAGGAAAAGA CAAGATTGTT TTTGGCAACT TACACCAGAT CTACGAGTGG
    CACAGAGACT TTTTTTTGGG TGAACTGGAA AGGTGCCTGG ATGAGCCTGA AAGATTGGCA
    TCCCTATTTG TCAAGCATGA AAGAAGATTG CATATGTACA TAGTTTACTG CCAAAATAAA
    CCAAAGTCTG AACATATTGT TTCAGAATAC ATTGATACCT ATTTTGAGGA CCTGAAACAG
    CGTCTGGGCC ATCGACTTCA GCTTACAGAT CTGTTAATAA AACCCATACA AAGAATAATG
    AAATATCAGC TGCTATTAAA GGATTTTCTC AAGTACTCTA AGAAGGCTTG CATAGATGTA
    GCAGAACTTG AGAGAGCTGT GGAAGTCATG TGTATCGTAC CGAAGCGGTG TAATGACATG
    ATGAATGTTG GTCGTTTGCA AGGATTTGAT GGCAAAATAG TTGCCCAAGG AAAGCTGCTA
    CTGCAAGACA CATTCCTTGT GACTGAGCAG GACGCTGGTC TTCTGCCCCG ATGCAAAGAA
    AGGCGGGTCT TCCTTTTCGA ACAAATTGTC ATCTTTAGTG AGCCCTTAGA TAAAAAGAAA
    GGATACTCTA CACCTGGATT TCTTTTTAAA AATAATATCA AAGTGAGCTG CCTTAGTTTA
    GAGGAGTGTG TTGATAGTGA TCCATGCAAG TTTGCACTAT TGTCAAGGTC TGCTGACTCT
    ACAGAGATGT TTGTTTTGCA TTCTTCATCG CCTGGAGTGA GACAGTTATG GATTCATGAA
    ATCAACCAAA TTTTGGAAAA CCAGCGCAAT TTTTTAAATG CCTTGACATC ACCAATTGAA
    TACCACAGAA ATCACACTGG TGGAAGCAGT AGTGGCAACG TCACCTTTAC CAGCAGTCCC
    AGCAATTGTA GTGGGCTGGG TGGCTCCAGC CGAAGCAGGC CCTCTCGGAT TCCCCAGCCT
    GTTAGACACC ACTCTCCAGT CCTGGTCTCC TCTGCAGCTT CGGCACAGTC AGAGGCAGAC
    AAGATGTCAG GTATGTCAGC TCGCCGTGCA CCAGTTGTAC CTCTCAACCT CTCCTTAGAC
    AATAACCCCA GCCAGAAAGG TAGAGTAACT GCTGCTCCAG AGACAGGCGG GCGAGATAAA
    GAATCCAATT CAGTGCCAAA GATGAAAATG CTTGATGGCC CCAGGAAATC AGCTAGTGGC
    ATTCCAACCT TAGCTGATGG TGCACAGAAA GAATTGCGTG GGAGCACAGA AGACAGTCGA
    ATTAGAAGCA CTATGGGCAC TCTAACCTTA GGAAAGCCAA GAAATGGTTC TGCCTCACCC
    CTCAACTCTC CTGTCACAAT TAATTTTCCA TCGTCTTTTG GTAAGGAATC AGGGCCACCA
    AGCAGTCCCC TTCAGAAAGG CTCTTTTTGG AGCTCAATTC CAGGATCTCC AGCCAGCCGA
    CCTGGCTCCT TCACTTTCCC TGGAGACAGT GACTCTCTCC AGCGACAAGT TCATCGGCAC
    TCCACACACA GCAAGGATAC AGACCGCATG AGCACCTGCT CCTCGACTAG TGAGCAGTCC
    GTTCACTCCA CTCAGAGTAA TGGGGTAAGG ACAGAGAGTG AAAGTAGCAG CAGTAGCAAT
    ATCTCCACCA TGCTGGTTAC GCACGATTAT ACAGCGGTGA AGGAGGATGA GATCAATGTC
    TACCAGGGAG AGGTTGTACA AATTCTGGCA AGTAACCAAC AGAACATGTT TCTGGTGTTC
    AGAGCTGCCA CTGATGAGTG CCCTGCAGCT GAGGGCTGGA TTCCAAGCTA TGTTCTGGGT
    CACACAAGTG CAATCATCAC TGATATGACT GATGCAACTA TGAAGAAGTC CTCTTCTTGG
    CATACAGCCC TTAGAATAAG AAAGAAATCT GAGAAGAGGG AAAAAGATGG CAGCAGAGAC
    ACAAAACTAG AGAATGGTTA TCGCAAATCA AGAGAAGGAC TAGCTAATAA GGTATCAGTT
    AAGCTCCTGA ATCCAAACTA CATTTATGAT GCTCCTCCAG AGTTTATTGT ACCACTGAAC
    GAGGTTACAT GTGACACAGG AGAGACTGTG ATTATTCGAT GCAAAGTTTG TGGTCGCCCC
    AAGGCATCCA TTACATGGAA AGGTCCCGAC CATAATACTT TGAGCAACGA TGGACACTAC
    AGTATATCCT ACAGTGATCT TGGAGAAGCA ACTCTAAAAA TAGTTGGTGT GACCACTGAG
    GATGATGGTA TATACACCTG CATTGCAACA AATGACTTGG GTTCAGTATC CGCTTCCTCC
    AATCTTAGAG TGTTAGGTTC ATGGAGTGAT GGACTGAGAG TAATCTGGAA AGAAAACTTC
    GACATTGCTT ATAGTGAAGT GGCTGAACTT GGCAGGGGCA GGTTTTCTGT CGTGAAAAAA
    TGTGAACAAA GAGTTACAAG ACGCATAGTA GCCACCAAAT TTGTTAACAA GAAGTTGATG
    AAAAGAGACC AGGTTACCCA TGAACTTGGA ATTCTGCAGA GCCTTCAGCA CCCACAACTG
    ATCAGCCTTC TTGACACTTT TGAGACACCA GCAAGCTATG TCTTAGTACT TGAGATAGCT
    GATCAGGGAT GTTTGTTAGA ATATGTGGTA CAGTGGGGAA ACCTTACAGA AGAAAAGATC
    ACCTCATATA TGAGAGAGGT GCTGGAGGCC CTGCAGTATC TTCATAATTG CAGAATAGCA
    CATCTGGACC TAAAGCCTGA GAACATTCTT GTGGATCAGA GCCTAACCAA ACCAACTATA
    AAACTGTCAG ACTTTGGCGA TGCTGTTCAG CTTAATACCA CTTACTACAT TCACCAATTG
    TTGGGCAGTG CAGAGTTTGC CGCTCCAGAG ATCATCCTTG GGAATCCTGT CTCCCTTACC
    TCTGATATAT GGAGTATTGG AGTTCTTACT TATGTCCTTC TCAGTGGCGT CTCTCCTTTC
    CTTGATGAAA GTGTTGAAGA GACATGCCTG AACATTTGCC GACTAGACTT TAGTTTTCCC
    GATGACTACT TTAAGGGAGT AAGTCAGAAG GCCAAAGAAT TTGTATGTTT CTTACTGCAG
    GGTGACCAGA CAAAGCGCCC TTCTGCTGCT GTATGTCTAA AGGAACAGTG GTTGCAGCCA
    GGCCAGCACA AACGTAGTGG CACATTAGAC ATGTCAAGAC TGACCTCGTT TATTGAACGC
    CGTAAACACC AGAATGATGT TCGACCCATT GGTAGCATTA AAAACTTCCT TCAGAGTCGC
    CTTTTACCCA GAATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012819807.1
    CDS616..9873
    Translation

    Target ORF information:

    RefSeq Version XM_012964353.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012964353.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGAGCACAT CCAGCACCGC AAGCTCCGGT AGCGCAAATA CCGGTACCGG CAATGCCGTT 
    GGAAGCAGCG GAGGCAACAC CGGGGAGGGG GCGGAGGAGA CGGCCAAGGA CTCTGCAGAC
    ATAGCTGCCT TTTACAGATC CGGTTTTCGT AGAAATGATG AATTGAAAGC AATGGAGGTG
    TTGCCAATTT TAAAAGAAAA AGTGGCATAT CTATCAGGTG GCAGAGATAA ACGAGGTGGT
    CCTGTATTAA CATTTCCAGC ACGCAGCAAC CATGATCGAA TACGACAAGA GGATCTCCGA
    AGACTGATTT CATATCTTGC TTGTATTCCT AGTGAGGAGG TGTGCAAGCG AGGCTTCACA
    GTGATTGTAG ATATGCGTGG TTCCAAGTGG GATTCTATTA AACCCTTGCT GAAAATTTTA
    CAAGAATCCT TTCCATGCTG TATTCATGTT GCACTAATTA TCAAACCAGA TAACTTCTGG
    CAGAAGCAAA GAACTAATTT TGGAAGTTCT AAGTTTGAAT TTGAGACAAG TATGGTGTCT
    TTAGAAGGCC TTACCAAAGT AGTTGACCCT TCCCAGCTAA CTCCAGAATT TGAGGGTTGC
    TTGGAATACA ACCATGAAGA GTGGATAGAG ATCAGAGTAG CATTTGAGGA TTTTATTAAT
    AATGCAACTC ATATGCTGTC TCGATTAGAG GAATTACAGG AGATGCTTGC AATTAAAGAG
    TTGCCTCAGG ATTTAGAGGG GGCACGCAAT ATGATTGAAG AACATTCTCA AATGAAAAAA
    AGGGTTATAA AGGCTCCTAT TGAAGATCTT GATTTAGAAG GGCAAAAGCT TTTACAAACA
    ATACAAAGCA GTGATAGCTT TCCTAAGAAG AACTCTAGTT CTGGAAATGC AGACCTTCAA
    AATCTTCTTC CCAAAGTTTC CACAATGTTA GACAGGCTTC ATTCAACACG ACAACATCTT
    CATCAGATGT GGCACATGCG AAAAGTAAAA CTGGACCAGT GCTTTCAGCT AAGGTTGTTT
    GAGCAGGATG CTGAGAAGAT GTTTGACTGG ATTACACACA ACAAAGGTCT GTTTCTGACA
    AGTTATACTG ACATTGGCAC CCGTAATAAT CATGCTATGG AACTTCAGAC ACAGCACAAT
    CATTTTGCTA TGAACTGCAT GAATGTCTAT GTAAACATTA GCCGAATAAT GTCTGTGGCA
    AATCGACTAG TAGAATCTGG ACACTATGCC TCACAGCAAA TTAAGCAGAT AGCTAACCAG
    TTGGAGCAAG AGTGGAAGGC TTTTGCCGCA GCCCTTGATG AACGTAGTAC GTTGCTTGAC
    ATGTCTGCAA TGTTCCATCA AAAAGCGGAG CAGTACATGG GCAATGTGGA TTCATGGTGC
    AAAGCTTGTG GGGAAGTGGA TCTTCCATCA GAGCTGCAGG ATCTAGAAGA TGCCATTCAT
    CACCATCAGG GAATATATGA GCATATCACT TTAGCATATT CAGAGGTTAG CCAGGATGGT
    AAATCTTTAT TAGACAAACT CCAGAGACCA TTGACTCCTG GTAGTTCTGA CTCACTGACA
    GCTTCTGCCA ACTATTCAAA GGCTGTGCAT CACGTCTTAG ATGCAATACA TGAAGTCTTG
    CACCACCAGC GTCAGTTGGA AAACATCTGG CAACATCGCA AAGTTCGTCT GCATCAACGC
    CTACAGCTCT GTGTTTTTCA GCAAGATGTT CAGCAGGTCT TGGATTGGAT AGAGAACCAT
    GGTGAAGCCT TTCTCAGCAA ACACACTGGG GTAGGCAAGT CTCTGCACAG GGCCAGAGCC
    TTGCAAAAGC GACATGAAGA CTTTGAAGAA GTCGCTCAGA ATACATACAC CAATGCTGAT
    AAGCTGCTGG AAGCTGCAGA ACAGTTGGCC CAGACAGGAG AGTGCGATCC AGAGGAAATA
    TACCAGGCTG CTCATCAGCT TGAGGATCGG ATTCAGGATT TTGTAAGACG TGTAGAACAA
    AGAAAAGTTC TCTTGGACAT GTCTGTATCA TTCCACTCGC ATGTTAAAGA GCTATGGACA
    TGGCTGGAGG ACCTACAAAA GGAACTGCTG GATGATGTCT ATGCGGAGTC AGTAGAAGCT
    GTACAGGACT TGATCAAACG GTTTGGACAA CAGCAGCAGA CAACCCTCCA GTTTACAGTA
    AATGTCATCA AAGAAGGGGA GGATCTAATT CAGCAACTAA GAGATTCTGC TATATCCAGC
    AACAAAACTC CTCACAACAG CTCAATCAAT CACATTGAGA CTGTCCTTCA ACAACTTGAT
    GAGGCCCAAT CACAGATGGA GGAGCTTTTC CAAGAGAGAA AAATCAAACT TGAGCTATTC
    TTGCAGCTTC GCATCTTTGA GAGAGATGCC ATTGATATCA TCTCAGACTT GGAATCATGG
    AATGATGAAC TTTCTCAGCA AATGAATGAT TTTGACACAG AGGACCTCAC ACTAGCCGAG
    CAAAGGCTAC AGCATCACGC AGACAAAGCT CTGACAATGA ACAACCTGAC ATTTGATGTC
    ATCCACCAAG GACAAGAGTT ACTACAATAT GTGAATGAAG TTCAGAACTC AGGGGTTGAG
    CTACTCTGTG ATAGAGATGT AGATATGGCT ACTAGAGTTC AGGACCTGTT GGAATTCCTC
    CATGAGAAAC AACAGGAGTT GGATCTAGCA GCTGAGCAGC ACCGAAAACA TCTAGAACAA
    TGTGTGCAAC TACGACATTT GCAAGCTGAA GTCAAACAGG TACTCGGCTG GATACGGAAT
    GGAGAGTCTA TGTTAAATGC CGGGCTTATC ACTGCCAGTT CATTGCAAGA AGCAGAGCAA
    CTTCAAAGGG AACATGAGCA GTTCCAGCAT GCAATAGAAA AAACCCACCA GAGTGCTCTG
    CAGGTGCAGC AGAAGGCAGA GGCCATGCTT CAAGCAAACC ATTACGACAT GGATATGATC
    CGGGACTGTG CTGAAAAAGT GGCCTCTCAT TGGCAACAGT TGATGCTTAA AATGGAAGAC
    CGTCTTAAGC TTGTCAATGC CTCTGTGGCC TTTTATAAAA CCTCAGAGCA GGTATGCAGT
    GTTCTAGAGA GCCTAGAGCA GGAGTACAAG CGGGAGGAAG ATTGGTGTGG CGGAGCTGAT
    AAACTTGGGC CTAACTCAGA GACTGACCAT GTTACTCCTA TGATCAGCAA ACACCTTGAA
    CAAAAGGAAG CCTTTCTTAA GGCCTGCACT TTGGCTCGCA GAAATGCTGA TGTCTTTCTG
    AAATACTTGC ACAGGAATAG TGTCAATATG CCTGGAATGG TGACACATGT AAAGGCTCCT
    GAACAGCAAG TAAAAAATAT TTTAAATGAG CTGTTTCAGA GGGAAAATAG AGTTCTTCAT
    TATTGGACCT TGCGGAAAAG GAGACTAGAC CATTGCCAGC AGTATGTAGT ATTTGAAAGA
    AGTGCCAAAC AGGCTCTGGA GTGGATCCAA GATAATGGAG AATTTTACAT TTCCACACAC
    ACCTCCACTG GCTCCACAAT TCAGCATACA CAAGAACTCT TAAAAGAGCA TGAGGAGTTT
    CAGATCACAG CAAAGCAAAC CAAAGAGAGG GTAAAGCTGT TGATACAGCT GGCTGATGGC
    TTTTGTGAAA AAGGGCATGC TCATGCTGCA GAAATTAAAA AATGTGTTAC AGCGGTGGAC
    AAGAGGTACA GAGACTTCTC GCTGCGTATG GAAAAGTACA GAACCAGCTT AGAGAAGGCT
    TTGGGCATTT CTTCTGATTC CAAGTCGAGT AAAAGCCTTC AGTTGGATAT TATTCCAGCC
    ACTGCCCCTG GTTCCGAAGT CAAGCTTCGT GATGCAACAC ATGAACTCAA TGAGGAGAAA
    AGAAAATCTG CTCGTCGAAA AGAATTCATC ATGGCAGAAC TTATACAAAC TGAAAAAGCA
    TATGTTCGTG ATCTTCGGGA ATGCATGGAT ACATATCTGT GGGAAATGAC AAGCGGAGTT
    GAAGAGATCC CACCAGGCAT TGTAAATAAA GAACACATTA TCTTTGGAAA CATGCAGGAA
    ATATATGAAT TTCATAACAA CATATTCTTA AAGGAGCTTG AGAAGTATGA GCAGCTACCT
    GAAGATGTTG GACATTGTTT TGTCACATGG GCTGACAAAT TTCAGATGTA TGTGTCCTAC
    TGTAAGAATA AGCCTGATTC AACACAACTT ATCTTGGAAC AAGCAGGAAC ATATTTTGAT
    GAAATTCAGC AGCGCCATGA GCTGGTCAAC TCCATCTCTT CTTATCTTAT CAAACCTGTG
    CAAAGGATAA CAAAATATCA GCTTCTTTTA AAAGAGCTGC TGACATGCTG CGAAGAAGGT
    AAAGGAGAGA TTAAAGATGG GCTGGAAGTG ATGCTAAGTG TACCAAAAAG AGCCAACGAT
    GCAATGCATC TCAGCATGCT TGAAGGGTTT GATGAGAACA TTGAATCTCA GGGAGAACTT
    ATTCTGCAAG AGTCTTTTCA AGTGTGGGAT CCTAAAACCT TAATAAGAAA GGGGCGTGAG
    AGACATCTCT TCTTGTTTGA AATGTCTTTG GTCTTCAGCA AAGAAGTGAA AGATTCAAGT
    GGAAGGAGCA AATATATTTA CAAATCAAAA TTATTTACCT CAGAGCTTGG AGTCACAGAA
    CATGTAGAAG GTGATCCTTG CAAATTTGCA CTTTGGGTAG GCAGAACGCC AACATCTGAT
    AACAAAATAG TCCTTAAGGG CTCAAGTATT GAGAACAAGC AAGATTGGAT CAAGCATATA
    AGAGAAGTGA TCCAAGACCG AACCATTCAC CTGAGAGGCG CATTAAAAGA GCCTATTCAT
    ATACCTAAAA CAGCTGCTGC TACAAAACAG AAACCTGCCA AAAGAGATGG AGAAGACCTG
    GACAGTCAAG GAGATGGCAG CAGCCAACCT GATACAATAT CTATTGCTTC ACGAACTTCA
    CAGAACACAT TAGACAGTGA TAAGCTTTCT GGTGGATGTG AGCTGACAGT AGTCATCCAT
    GACTTTACTG CCTGTAATAG CAATGAGCTG ACAATTAAAC GAGGGCAGAC TGTGGAGGTT
    CTAGAGCGGC ATCACGATCG ACCTGACTGG TGTTTAGTTC GGACTACTGA CCGGTCACCT
    GCAGCAGAGG GCTTGGTGCC TAGTAGTTCA CTGTGTATTG CACATTCTAG AAGTAGCATG
    GAAATGGAAG GCATATTTAA CCACAAAGAT TCCCTCTCTG TCTCCAGTAA TGATGCCAGT
    CCTCCTGCTT CAGTAATTTC CCTTCAGCCT CACATGATTG GTGCTCAAAG CTCTCCTGGC
    CCAAAGCGAC CTGGCAATAC TTTGAGAAAA TGGCTCACCA GCCCTGTCAG GAGACTTAGC
    AGTGGCAAAG CTGATGGACA TGTGAAAAAA TTGGCTCATA AACACAAAAA AAGCAGGGAG
    GTTCGTAAAA ACACAGACTC AGGTTCCCAA AAGGACTCTG ATGACAGTGC AGCAACACCT
    CAAGATGAAA CTATCGAGGA GAGAGTGCGC AATGAAGGAC TTAGCAGTGG TACGCTTTCT
    AAATCCTCCT CGTCTGGCAT GCAAAGCTGT GGAGAAGAAG AGGGCGAGGA AGGACCTGAT
    GCAGTTCCAT TGCCTCCACC CATGGCTATT CAGCAGCACA GCCTTCTTCA GCCTGACTCA
    CAGGATGACA AGACATCATC TCGGTTGCTG GTGCGACCTA CTAGCTCAGA GACACCTAGT
    GCTGCAGAGC TTGTTAGTGC TATTGAAGAA CTTGTAAAGA GTAAAATGGC TTTGGAAGAC
    CGGCCAAGTT CACTTCAAGT AGACCAGGGA GACAGTAGTA GCCCATCATT CAACCCTTCA
    GATACATCTC TGCTTTCCTC CTCTTCTCCA ATTGATGAAA TGGAAGAAAA AAAATCAAGC
    TCTTTAAAAA GAAGGCACTA TGTATTACAA GAGCTAGTAG AAACTGAACG CGATTATGTT
    CGGGACCTTG GCTATGTGGT TGAGGGTTAT ATGGCACTTA TGAAGGAGGA TGGAGTTCCA
    GATGATATGA AAGGAAAAGA CAAGATTGTT TTTGGCAACT TACACCAGAT CTACGAGTGG
    CACAGAGACT TTTTTTTGGG TGAACTGGAA AGGTGCCTGG ATGAGCCTGA AAGATTGGCA
    TCCCTATTTG TCAAGCATGA AAGAAGATTG CATATGTACA TAGTTTACTG CCAAAATAAA
    CCAAAGTCTG AACATATTGT TTCAGAATAC ATTGATACCT ATTTTGAGGA CCTGAAACAG
    CGTCTGGGCC ATCGACTTCA GCTTACAGAT CTGTTAATAA AACCCATACA AAGAATAATG
    AAATATCAGC TGCTATTAAA GGATTTTCTC AAGTACTCTA AGAAGGCTTG CATAGATGTA
    GCAGAACTTG AGAGAGCTGT GGAAGTCATG TGTATCGTAC CGAAGCGGTG TAATGACATG
    ATGAATGTTG GTCGTTTGCA AGGATTTGAT GGCAAAATAG TTGCCCAAGG AAAGCTGCTA
    CTGCAAGACA CATTCCTTGT GACTGAGCAG GACGCTGGTC TTCTGCCCCG ATGCAAAGAA
    AGGCGGGTCT TCCTTTTCGA ACAAATTGTC ATCTTTAGTG AGCCCTTAGA TAAAAAGAAA
    GGATACTCTA CACCTGGATT TCTTTTTAAA AATAATATCA AAGTGAGCTG CCTTAGTTTA
    GAGGAGTGTG TTGATAGTGA TCCATGCAAG TTTGCACTAT TGTCAAGGTC TGCTGACTCT
    ACAGAGATGT TTGTTTTGCA TTCTTCATCG CCTGGAGTGA GACAGTTATG GATTCATGAA
    ATCAACCAAA TTTTGGAAAA CCAGCGCAAT TTTTTAAATG CCTTGACATC ACCAATTGAA
    TACCACAGAA ATCACACTGG TGGAAGCAGT AGTGGCAACG TCACCTTTAC CAGCAGTCCC
    AGCAATTGTA GTGGGCTGGG TGGCTCCAGC CGAAGCAGGC CCTCTCGGAT TCCCCAGCCT
    GTTAGACACC ACTCTCCAGT CCTGGTCTCC TCTGCAGCTT CGGCACAGTC AGAGGCAGAC
    AAGATGTCAG GTATGTCAGC TCGCCGTGCA CCAGTTGTAC CTCTCAACCT CTCCTTAGAC
    AATAACCCCA GCCAGAAAGG TAGAGTAACT GCTGCTCCAG AGACAGGCGG GCGAGATAAA
    GAATCCAATT CAGTGCCAAA GATGAAAATG CTTGATGGCC CCAGGAAATC AGCTAGTGGC
    ATTCCAACCT TAGCTGATGG TGCACAGAAA GAATTGCGTG GGAGCACAGA AGACAGTCGA
    ATTAGAAGCA CTATGGGCAC TCTAACCTTA GGAAAGCCAA GAAATGGTTC TGCCTCACCC
    CTCAACTCTC CTGTCACAAT TAATTTTCCA TCGTCTTTTG GTAAGGAATC AGGGCCACCA
    AGCAGTCCCC TTCAGAAAGG CTCTTTTTGG AGCTCAATTC CAGGATCTCC AGCCAGCCGA
    CCTGGCTCCT TCACTTTCCC TGGAGACAGT GACTCTCTCC AGCGACAAGT TCATCGGCAC
    TCCACACACA GCAAGGATAC AGACCGCATG AGCACCTGCT CCTCGACTAG TGAGCAGTCC
    GTTCACTCCA CTCAGAGTAA TGGGGTAAGG ACAGAGAGTG AAAGTAGCAG CAGTAGCAAT
    ATCTCCACCA TGCTGGTTAC GCACGATTAT ACAGCGGTGA AGGAGGATGA GATCAATGTC
    TACCAGGGAG AGGTTGTACA AATTCTGGCA AGTAACCAAC AGAACATGTT TCTGGTGTTC
    AGAGCTGCCA CTGATGAGTG CCCTGCAGCT GAGGGCTGGA TTCCAAGCTA TGTTCTGGGT
    CACACAAGTG CAATCATCAC TGATATGACT GATGCAACTA TGAAGAAGTC CTCTTCTTGG
    CATACAGCCC TTAGAATAAG AAAGAAATCT GAGAAGAGGG AAAAAGATGG CAGCAGAGAC
    ACAAAACTAG AGAATGGTTA TCGCAAATCA AGAGAAGGAC TAGCTAATAA GGTATCAGTT
    AAGCTCCTGA ATCCAAACTA CATTTATGAT GCTCCTCCAG AGTTTATTGT ACCACTGAAC
    GAGGTTACAT GTGACACAGG AGAGACTGTG ATTATTCGAT GCAAAGTTTG TGGTCGCCCC
    AAGGCATCCA TTACATGGAA AGGTCCCGAC CATAATACTT TGAGCAACGA TGGACACTAC
    AGTATATCCT ACAGTGATCT TGGAGAAGCA ACTCTAAAAA TAGTTGGTGT GACCACTGAG
    GATGATGGTA TATACACCTG CATTGCAACA AATGACTTGG GTTCAGTATC CGCTTCCTCC
    AATCTTAGAG TGTTAGGTTC ATGGAGTGAT GGACTGAGAG TAATCTGGAA AGAAAACTTC
    GACATTGCTT ATAGTGAAGT GGCTGAACTT GGCAGGGGCA GGTTTTCTGT CGTGAAAAAA
    TGTGAACAAA GAGTTACAAG ACGCATAGTA GCCACCAAAT TTGTTAACAA GAAGTTGATG
    AAAAGAGACC AGGTTACCCA TGAACTTGGA ATTCTGCAGA GCCTTCAGCA CCCACAACTG
    ATCAGCCTTC TTGACACTTT TGAGACACCA GCAAGCTATG TCTTAGTACT TGAGATAGCT
    GATCAGGGAT GTTTGTTAGA ATATGTGGTA CAGTGGGGAA ACCTTACAGA AGAAAAGATC
    ACCTCATATA TGAGAGAGGT GCTGGAGGCC CTGCAGTATC TTCATAATTG CAGAATAGCA
    CATCTGGACC TAAAGCCTGA GAACATTCTT GTGGATCAGA GCCTAACCAA ACCAACTATA
    AAACTGTCAG ACTTTGGCGA TGCTGTTCAG CTTAATACCA CTTACTACAT TCACCAATTG
    TTGGGCAGTG CAGAGTTTGC CGCTCCAGAG ATCATCCTTG GGAATCCTGT CTCCCTTACC
    TCTGATATAT GGAGTATTGG AGTTCTTACT TATGTCCTTC TCAGTGGCGT CTCTCCTTTC
    CTTGATGAAA GTGTTGAAGA GACATGCCTG AACATTTGCC GACTAGACTT TAGTTTTCCC
    GATGACTACT TTAAGGGAGT AAGTCAGAAG GCCAAAGAAT TTGTATGTTT CTTACTGCAG
    GGTGACCAGA CAAAGCGCCC TTCTGCTGCT GTATGTCTAA AGGAACAGTG GTTGCAGCCA
    GGCCAGCACA AACGTAGTGG CACATTAGAC ATGTCAAGAC TGACCTCGTT TATTGAACGC
    CGTAAACACC AGAATGATGT TCGACCCATT GGTAGCATTA AAAACTTCCT TCAGAGTCGC
    CTTTTACCCA GAATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32253
    Clone ID Related Accession (Same CDS sequence) XM_012964353.2 , XM_012964353.3
    Accession Version XM_012964353.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9258bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product triple functional domain protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012964353.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGAGCACAT CCAGCACCGC AAGCTCCGGT AGCGCAAATA CCGGTACCGG CAATGCCGTT 
    GGAAGCAGCG GAGGCAACAC CGGGGAGGGG GCGGAGGAGA CGGCCAAGGA CTCTGCAGAC
    ATAGCTGCCT TTTACAGATC CGGTTTTCGT AGAAATGATG AATTGAAAGC AATGGAGGTG
    TTGCCAATTT TAAAAGAAAA AGTGGCATAT CTATCAGGTG GCAGAGATAA ACGAGGTGGT
    CCTGTATTAA CATTTCCAGC ACGCAGCAAC CATGATCGAA TACGACAAGA GGATCTCCGA
    AGACTGATTT CATATCTTGC TTGTATTCCT AGTGAGGAGG TGTGCAAGCG AGGCTTCACA
    GTGATTGTAG ATATGCGTGG TTCCAAGTGG GATTCTATTA AACCCTTGCT GAAAATTTTA
    CAAGAATCCT TTCCATGCTG TATTCATGTT GCACTAATTA TCAAACCAGA TAACTTCTGG
    CAGAAGCAAA GAACTAATTT TGGAAGTTCT AAGTTTGAAT TTGAGACAAG TATGGTGTCT
    TTAGAAGGCC TTACCAAAGT AGTTGACCCT TCCCAGCTAA CTCCAGAATT TGAGGGTTGC
    TTGGAATACA ACCATGAAGA GTGGATAGAG ATCAGAGTAG CATTTGAGGA TTTTATTAAT
    AATGCAACTC ATATGCTGTC TCGATTAGAG GAATTACAGG AGATGCTTGC AATTAAAGAG
    TTGCCTCAGG ATTTAGAGGG GGCACGCAAT ATGATTGAAG AACATTCTCA AATGAAAAAA
    AGGGTTATAA AGGCTCCTAT TGAAGATCTT GATTTAGAAG GGCAAAAGCT TTTACAAACA
    ATACAAAGCA GTGATAGCTT TCCTAAGAAG AACTCTAGTT CTGGAAATGC AGACCTTCAA
    AATCTTCTTC CCAAAGTTTC CACAATGTTA GACAGGCTTC ATTCAACACG ACAACATCTT
    CATCAGATGT GGCACATGCG AAAAGTAAAA CTGGACCAGT GCTTTCAGCT AAGGTTGTTT
    GAGCAGGATG CTGAGAAGAT GTTTGACTGG ATTACACACA ACAAAGGTCT GTTTCTGACA
    AGTTATACTG ACATTGGCAC CCGTAATAAT CATGCTATGG AACTTCAGAC ACAGCACAAT
    CATTTTGCTA TGAACTGCAT GAATGTCTAT GTAAACATTA GCCGAATAAT GTCTGTGGCA
    AATCGACTAG TAGAATCTGG ACACTATGCC TCACAGCAAA TTAAGCAGAT AGCTAACCAG
    TTGGAGCAAG AGTGGAAGGC TTTTGCCGCA GCCCTTGATG AACGTAGTAC GTTGCTTGAC
    ATGTCTGCAA TGTTCCATCA AAAAGCGGAG CAGTACATGG GCAATGTGGA TTCATGGTGC
    AAAGCTTGTG GGGAAGTGGA TCTTCCATCA GAGCTGCAGG ATCTAGAAGA TGCCATTCAT
    CACCATCAGG GAATATATGA GCATATCACT TTAGCATATT CAGAGGTTAG CCAGGATGGT
    AAATCTTTAT TAGACAAACT CCAGAGACCA TTGACTCCTG GTAGTTCTGA CTCACTGACA
    GCTTCTGCCA ACTATTCAAA GGCTGTGCAT CACGTCTTAG ATGCAATACA TGAAGTCTTG
    CACCACCAGC GTCAGTTGGA AAACATCTGG CAACATCGCA AAGTTCGTCT GCATCAACGC
    CTACAGCTCT GTGTTTTTCA GCAAGATGTT CAGCAGGTCT TGGATTGGAT AGAGAACCAT
    GGTGAAGCCT TTCTCAGCAA ACACACTGGG GTAGGCAAGT CTCTGCACAG GGCCAGAGCC
    TTGCAAAAGC GACATGAAGA CTTTGAAGAA GTCGCTCAGA ATACATACAC CAATGCTGAT
    AAGCTGCTGG AAGCTGCAGA ACAGTTGGCC CAGACAGGAG AGTGCGATCC AGAGGAAATA
    TACCAGGCTG CTCATCAGCT TGAGGATCGG ATTCAGGATT TTGTAAGACG TGTAGAACAA
    AGAAAAGTTC TCTTGGACAT GTCTGTATCA TTCCACTCGC ATGTTAAAGA GCTATGGACA
    TGGCTGGAGG ACCTACAAAA GGAACTGCTG GATGATGTCT ATGCGGAGTC AGTAGAAGCT
    GTACAGGACT TGATCAAACG GTTTGGACAA CAGCAGCAGA CAACCCTCCA GTTTACAGTA
    AATGTCATCA AAGAAGGGGA GGATCTAATT CAGCAACTAA GAGATTCTGC TATATCCAGC
    AACAAAACTC CTCACAACAG CTCAATCAAT CACATTGAGA CTGTCCTTCA ACAACTTGAT
    GAGGCCCAAT CACAGATGGA GGAGCTTTTC CAAGAGAGAA AAATCAAACT TGAGCTATTC
    TTGCAGCTTC GCATCTTTGA GAGAGATGCC ATTGATATCA TCTCAGACTT GGAATCATGG
    AATGATGAAC TTTCTCAGCA AATGAATGAT TTTGACACAG AGGACCTCAC ACTAGCCGAG
    CAAAGGCTAC AGCATCACGC AGACAAAGCT CTGACAATGA ACAACCTGAC ATTTGATGTC
    ATCCACCAAG GACAAGAGTT ACTACAATAT GTGAATGAAG TTCAGAACTC AGGGGTTGAG
    CTACTCTGTG ATAGAGATGT AGATATGGCT ACTAGAGTTC AGGACCTGTT GGAATTCCTC
    CATGAGAAAC AACAGGAGTT GGATCTAGCA GCTGAGCAGC ACCGAAAACA TCTAGAACAA
    TGTGTGCAAC TACGACATTT GCAAGCTGAA GTCAAACAGG TACTCGGCTG GATACGGAAT
    GGAGAGTCTA TGTTAAATGC CGGGCTTATC ACTGCCAGTT CATTGCAAGA AGCAGAGCAA
    CTTCAAAGGG AACATGAGCA GTTCCAGCAT GCAATAGAAA AAACCCACCA GAGTGCTCTG
    CAGGTGCAGC AGAAGGCAGA GGCCATGCTT CAAGCAAACC ATTACGACAT GGATATGATC
    CGGGACTGTG CTGAAAAAGT GGCCTCTCAT TGGCAACAGT TGATGCTTAA AATGGAAGAC
    CGTCTTAAGC TTGTCAATGC CTCTGTGGCC TTTTATAAAA CCTCAGAGCA GGTATGCAGT
    GTTCTAGAGA GCCTAGAGCA GGAGTACAAG CGGGAGGAAG ATTGGTGTGG CGGAGCTGAT
    AAACTTGGGC CTAACTCAGA GACTGACCAT GTTACTCCTA TGATCAGCAA ACACCTTGAA
    CAAAAGGAAG CCTTTCTTAA GGCCTGCACT TTGGCTCGCA GAAATGCTGA TGTCTTTCTG
    AAATACTTGC ACAGGAATAG TGTCAATATG CCTGGAATGG TGACACATGT AAAGGCTCCT
    GAACAGCAAG TAAAAAATAT TTTAAATGAG CTGTTTCAGA GGGAAAATAG AGTTCTTCAT
    TATTGGACCT TGCGGAAAAG GAGACTAGAC CATTGCCAGC AGTATGTAGT ATTTGAAAGA
    AGTGCCAAAC AGGCTCTGGA GTGGATCCAA GATAATGGAG AATTTTACAT TTCCACACAC
    ACCTCCACTG GCTCCACAAT TCAGCATACA CAAGAACTCT TAAAAGAGCA TGAGGAGTTT
    CAGATCACAG CAAAGCAAAC CAAAGAGAGG GTAAAGCTGT TGATACAGCT GGCTGATGGC
    TTTTGTGAAA AAGGGCATGC TCATGCTGCA GAAATTAAAA AATGTGTTAC AGCGGTGGAC
    AAGAGGTACA GAGACTTCTC GCTGCGTATG GAAAAGTACA GAACCAGCTT AGAGAAGGCT
    TTGGGCATTT CTTCTGATTC CAAGTCGAGT AAAAGCCTTC AGTTGGATAT TATTCCAGCC
    ACTGCCCCTG GTTCCGAAGT CAAGCTTCGT GATGCAACAC ATGAACTCAA TGAGGAGAAA
    AGAAAATCTG CTCGTCGAAA AGAATTCATC ATGGCAGAAC TTATACAAAC TGAAAAAGCA
    TATGTTCGTG ATCTTCGGGA ATGCATGGAT ACATATCTGT GGGAAATGAC AAGCGGAGTT
    GAAGAGATCC CACCAGGCAT TGTAAATAAA GAACACATTA TCTTTGGAAA CATGCAGGAA
    ATATATGAAT TTCATAACAA CATATTCTTA AAGGAGCTTG AGAAGTATGA GCAGCTACCT
    GAAGATGTTG GACATTGTTT TGTCACATGG GCTGACAAAT TTCAGATGTA TGTGTCCTAC
    TGTAAGAATA AGCCTGATTC AACACAACTT ATCTTGGAAC AAGCAGGAAC ATATTTTGAT
    GAAATTCAGC AGCGCCATGA GCTGGTCAAC TCCATCTCTT CTTATCTTAT CAAACCTGTG
    CAAAGGATAA CAAAATATCA GCTTCTTTTA AAAGAGCTGC TGACATGCTG CGAAGAAGGT
    AAAGGAGAGA TTAAAGATGG GCTGGAAGTG ATGCTAAGTG TACCAAAAAG AGCCAACGAT
    GCAATGCATC TCAGCATGCT TGAAGGGTTT GATGAGAACA TTGAATCTCA GGGAGAACTT
    ATTCTGCAAG AGTCTTTTCA AGTGTGGGAT CCTAAAACCT TAATAAGAAA GGGGCGTGAG
    AGACATCTCT TCTTGTTTGA AATGTCTTTG GTCTTCAGCA AAGAAGTGAA AGATTCAAGT
    GGAAGGAGCA AATATATTTA CAAATCAAAA TTATTTACCT CAGAGCTTGG AGTCACAGAA
    CATGTAGAAG GTGATCCTTG CAAATTTGCA CTTTGGGTAG GCAGAACGCC AACATCTGAT
    AACAAAATAG TCCTTAAGGG CTCAAGTATT GAGAACAAGC AAGATTGGAT CAAGCATATA
    AGAGAAGTGA TCCAAGACCG AACCATTCAC CTGAGAGGCG CATTAAAAGA GCCTATTCAT
    ATACCTAAAA CAGCTGCTGC TACAAAACAG AAACCTGCCA AAAGAGATGG AGAAGACCTG
    GACAGTCAAG GAGATGGCAG CAGCCAACCT GATACAATAT CTATTGCTTC ACGAACTTCA
    CAGAACACAT TAGACAGTGA TAAGCTTTCT GGTGGATGTG AGCTGACAGT AGTCATCCAT
    GACTTTACTG CCTGTAATAG CAATGAGCTG ACAATTAAAC GAGGGCAGAC TGTGGAGGTT
    CTAGAGCGGC ATCACGATCG ACCTGACTGG TGTTTAGTTC GGACTACTGA CCGGTCACCT
    GCAGCAGAGG GCTTGGTGCC TAGTAGTTCA CTGTGTATTG CACATTCTAG AAGTAGCATG
    GAAATGGAAG GCATATTTAA CCACAAAGAT TCCCTCTCTG TCTCCAGTAA TGATGCCAGT
    CCTCCTGCTT CAGTAATTTC CCTTCAGCCT CACATGATTG GTGCTCAAAG CTCTCCTGGC
    CCAAAGCGAC CTGGCAATAC TTTGAGAAAA TGGCTCACCA GCCCTGTCAG GAGACTTAGC
    AGTGGCAAAG CTGATGGACA TGTGAAAAAA TTGGCTCATA AACACAAAAA AAGCAGGGAG
    GTTCGTAAAA ACACAGACTC AGGTTCCCAA AAGGACTCTG ATGACAGTGC AGCAACACCT
    CAAGATGAAA CTATCGAGGA GAGAGTGCGC AATGAAGGAC TTAGCAGTGG TACGCTTTCT
    AAATCCTCCT CGTCTGGCAT GCAAAGCTGT GGAGAAGAAG AGGGCGAGGA AGGACCTGAT
    GCAGTTCCAT TGCCTCCACC CATGGCTATT CAGCAGCACA GCCTTCTTCA GCCTGACTCA
    CAGGATGACA AGACATCATC TCGGTTGCTG GTGCGACCTA CTAGCTCAGA GACACCTAGT
    GCTGCAGAGC TTGTTAGTGC TATTGAAGAA CTTGTAAAGA GTAAAATGGC TTTGGAAGAC
    CGGCCAAGTT CACTTCAAGT AGACCAGGGA GACAGTAGTA GCCCATCATT CAACCCTTCA
    GATACATCTC TGCTTTCCTC CTCTTCTCCA ATTGATGAAA TGGAAGAAAA AAAATCAAGC
    TCTTTAAAAA GAAGGCACTA TGTATTACAA GAGCTAGTAG AAACTGAACG CGATTATGTT
    CGGGACCTTG GCTATGTGGT TGAGGGTTAT ATGGCACTTA TGAAGGAGGA TGGAGTTCCA
    GATGATATGA AAGGAAAAGA CAAGATTGTT TTTGGCAACT TACACCAGAT CTACGAGTGG
    CACAGAGACT TTTTTTTGGG TGAACTGGAA AGGTGCCTGG ATGAGCCTGA AAGATTGGCA
    TCCCTATTTG TCAAGCATGA AAGAAGATTG CATATGTACA TAGTTTACTG CCAAAATAAA
    CCAAAGTCTG AACATATTGT TTCAGAATAC ATTGATACCT ATTTTGAGGA CCTGAAACAG
    CGTCTGGGCC ATCGACTTCA GCTTACAGAT CTGTTAATAA AACCCATACA AAGAATAATG
    AAATATCAGC TGCTATTAAA GGATTTTCTC AAGTACTCTA AGAAGGCTTG CATAGATGTA
    GCAGAACTTG AGAGAGCTGT GGAAGTCATG TGTATCGTAC CGAAGCGGTG TAATGACATG
    ATGAATGTTG GTCGTTTGCA AGGATTTGAT GGCAAAATAG TTGCCCAAGG AAAGCTGCTA
    CTGCAAGACA CATTCCTTGT GACTGAGCAG GACGCTGGTC TTCTGCCCCG ATGCAAAGAA
    AGGCGGGTCT TCCTTTTCGA ACAAATTGTC ATCTTTAGTG AGCCCTTAGA TAAAAAGAAA
    GGATACTCTA CACCTGGATT TCTTTTTAAA AATAATATCA AAGTGAGCTG CCTTAGTTTA
    GAGGAGTGTG TTGATAGTGA TCCATGCAAG TTTGCACTAT TGTCAAGGTC TGCTGACTCT
    ACAGAGATGT TTGTTTTGCA TTCTTCATCG CCTGGAGTGA GACAGTTATG GATTCATGAA
    ATCAACCAAA TTTTGGAAAA CCAGCGCAAT TTTTTAAATG CCTTGACATC ACCAATTGAA
    TACCACAGAA ATCACACTGG TGGAAGCAGT AGTGGCAACG TCACCTTTAC CAGCAGTCCC
    AGCAATTGTA GTGGGCTGGG TGGCTCCAGC CGAAGCAGGC CCTCTCGGAT TCCCCAGCCT
    GTTAGACACC ACTCTCCAGT CCTGGTCTCC TCTGCAGCTT CGGCACAGTC AGAGGCAGAC
    AAGATGTCAG GTATGTCAGC TCGCCGTGCA CCAGTTGTAC CTCTCAACCT CTCCTTAGAC
    AATAACCCCA GCCAGAAAGG TAGAGTAACT GCTGCTCCAG AGACAGGCGG GCGAGATAAA
    GAATCCAATT CAGTGCCAAA GATGAAAATG CTTGATGGCC CCAGGAAATC AGCTAGTGGC
    ATTCCAACCT TAGCTGATGG TGCACAGAAA GAATTGCGTG GGAGCACAGA AGACAGTCGA
    ATTAGAAGCA CTATGGGCAC TCTAACCTTA GGAAAGCCAA GAAATGGTTC TGCCTCACCC
    CTCAACTCTC CTGTCACAAT TAATTTTCCA TCGTCTTTTG GTAAGGAATC AGGGCCACCA
    AGCAGTCCCC TTCAGAAAGG CTCTTTTTGG AGCTCAATTC CAGGATCTCC AGCCAGCCGA
    CCTGGCTCCT TCACTTTCCC TGGAGACAGT GACTCTCTCC AGCGACAAGT TCATCGGCAC
    TCCACACACA GCAAGGATAC AGACCGCATG AGCACCTGCT CCTCGACTAG TGAGCAGTCC
    GTTCACTCCA CTCAGAGTAA TGGGGTAAGG ACAGAGAGTG AAAGTAGCAG CAGTAGCAAT
    ATCTCCACCA TGCTGGTTAC GCACGATTAT ACAGCGGTGA AGGAGGATGA GATCAATGTC
    TACCAGGGAG AGGTTGTACA AATTCTGGCA AGTAACCAAC AGAACATGTT TCTGGTGTTC
    AGAGCTGCCA CTGATGAGTG CCCTGCAGCT GAGGGCTGGA TTCCAAGCTA TGTTCTGGGT
    CACACAAGTG CAATCATCAC TGATATGACT GATGCAACTA TGAAGAAGTC CTCTTCTTGG
    CATACAGCCC TTAGAATAAG AAAGAAATCT GAGAAGAGGG AAAAAGATGG CAGCAGAGAC
    ACAAAACTAG AGAATGGTTA TCGCAAATCA AGAGAAGGAC TAGCTAATAA GGTATCAGTT
    AAGCTCCTGA ATCCAAACTA CATTTATGAT GCTCCTCCAG AGTTTATTGT ACCACTGAAC
    GAGGTTACAT GTGACACAGG AGAGACTGTG ATTATTCGAT GCAAAGTTTG TGGTCGCCCC
    AAGGCATCCA TTACATGGAA AGGTCCCGAC CATAATACTT TGAGCAACGA TGGACACTAC
    AGTATATCCT ACAGTGATCT TGGAGAAGCA ACTCTAAAAA TAGTTGGTGT GACCACTGAG
    GATGATGGTA TATACACCTG CATTGCAACA AATGACTTGG GTTCAGTATC CGCTTCCTCC
    AATCTTAGAG TGTTAGGTTC ATGGAGTGAT GGACTGAGAG TAATCTGGAA AGAAAACTTC
    GACATTGCTT ATAGTGAAGT GGCTGAACTT GGCAGGGGCA GGTTTTCTGT CGTGAAAAAA
    TGTGAACAAA GAGTTACAAG ACGCATAGTA GCCACCAAAT TTGTTAACAA GAAGTTGATG
    AAAAGAGACC AGGTTACCCA TGAACTTGGA ATTCTGCAGA GCCTTCAGCA CCCACAACTG
    ATCAGCCTTC TTGACACTTT TGAGACACCA GCAAGCTATG TCTTAGTACT TGAGATAGCT
    GATCAGGGAT GTTTGTTAGA ATATGTGGTA CAGTGGGGAA ACCTTACAGA AGAAAAGATC
    ACCTCATATA TGAGAGAGGT GCTGGAGGCC CTGCAGTATC TTCATAATTG CAGAATAGCA
    CATCTGGACC TAAAGCCTGA GAACATTCTT GTGGATCAGA GCCTAACCAA ACCAACTATA
    AAACTGTCAG ACTTTGGCGA TGCTGTTCAG CTTAATACCA CTTACTACAT TCACCAATTG
    TTGGGCAGTG CAGAGTTTGC CGCTCCAGAG ATCATCCTTG GGAATCCTGT CTCCCTTACC
    TCTGATATAT GGAGTATTGG AGTTCTTACT TATGTCCTTC TCAGTGGCGT CTCTCCTTTC
    CTTGATGAAA GTGTTGAAGA GACATGCCTG AACATTTGCC GACTAGACTT TAGTTTTCCC
    GATGACTACT TTAAGGGAGT AAGTCAGAAG GCCAAAGAAT TTGTATGTTT CTTACTGCAG
    GGTGACCAGA CAAAGCGCCC TTCTGCTGCT GTATGTCTAA AGGAACAGTG GTTGCAGCCA
    GGCCAGCACA AACGTAGTGG CACATTAGAC ATGTCAAGAC TGACCTCGTT TATTGAACGC
    CGTAAACACC AGAATGATGT TCGACCCATT GGTAGCATTA AAAACTTCCT TCAGAGTCGC
    CTTTTACCCA GAATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012819807.1
    CDS527..9784
    Translation

    Target ORF information:

    RefSeq Version XM_012964353.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012964353.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGAGCACAT CCAGCACCGC AAGCTCCGGT AGCGCAAATA CCGGTACCGG CAATGCCGTT 
    GGAAGCAGCG GAGGCAACAC CGGGGAGGGG GCGGAGGAGA CGGCCAAGGA CTCTGCAGAC
    ATAGCTGCCT TTTACAGATC CGGTTTTCGT AGAAATGATG AATTGAAAGC AATGGAGGTG
    TTGCCAATTT TAAAAGAAAA AGTGGCATAT CTATCAGGTG GCAGAGATAA ACGAGGTGGT
    CCTGTATTAA CATTTCCAGC ACGCAGCAAC CATGATCGAA TACGACAAGA GGATCTCCGA
    AGACTGATTT CATATCTTGC TTGTATTCCT AGTGAGGAGG TGTGCAAGCG AGGCTTCACA
    GTGATTGTAG ATATGCGTGG TTCCAAGTGG GATTCTATTA AACCCTTGCT GAAAATTTTA
    CAAGAATCCT TTCCATGCTG TATTCATGTT GCACTAATTA TCAAACCAGA TAACTTCTGG
    CAGAAGCAAA GAACTAATTT TGGAAGTTCT AAGTTTGAAT TTGAGACAAG TATGGTGTCT
    TTAGAAGGCC TTACCAAAGT AGTTGACCCT TCCCAGCTAA CTCCAGAATT TGAGGGTTGC
    TTGGAATACA ACCATGAAGA GTGGATAGAG ATCAGAGTAG CATTTGAGGA TTTTATTAAT
    AATGCAACTC ATATGCTGTC TCGATTAGAG GAATTACAGG AGATGCTTGC AATTAAAGAG
    TTGCCTCAGG ATTTAGAGGG GGCACGCAAT ATGATTGAAG AACATTCTCA AATGAAAAAA
    AGGGTTATAA AGGCTCCTAT TGAAGATCTT GATTTAGAAG GGCAAAAGCT TTTACAAACA
    ATACAAAGCA GTGATAGCTT TCCTAAGAAG AACTCTAGTT CTGGAAATGC AGACCTTCAA
    AATCTTCTTC CCAAAGTTTC CACAATGTTA GACAGGCTTC ATTCAACACG ACAACATCTT
    CATCAGATGT GGCACATGCG AAAAGTAAAA CTGGACCAGT GCTTTCAGCT AAGGTTGTTT
    GAGCAGGATG CTGAGAAGAT GTTTGACTGG ATTACACACA ACAAAGGTCT GTTTCTGACA
    AGTTATACTG ACATTGGCAC CCGTAATAAT CATGCTATGG AACTTCAGAC ACAGCACAAT
    CATTTTGCTA TGAACTGCAT GAATGTCTAT GTAAACATTA GCCGAATAAT GTCTGTGGCA
    AATCGACTAG TAGAATCTGG ACACTATGCC TCACAGCAAA TTAAGCAGAT AGCTAACCAG
    TTGGAGCAAG AGTGGAAGGC TTTTGCCGCA GCCCTTGATG AACGTAGTAC GTTGCTTGAC
    ATGTCTGCAA TGTTCCATCA AAAAGCGGAG CAGTACATGG GCAATGTGGA TTCATGGTGC
    AAAGCTTGTG GGGAAGTGGA TCTTCCATCA GAGCTGCAGG ATCTAGAAGA TGCCATTCAT
    CACCATCAGG GAATATATGA GCATATCACT TTAGCATATT CAGAGGTTAG CCAGGATGGT
    AAATCTTTAT TAGACAAACT CCAGAGACCA TTGACTCCTG GTAGTTCTGA CTCACTGACA
    GCTTCTGCCA ACTATTCAAA GGCTGTGCAT CACGTCTTAG ATGCAATACA TGAAGTCTTG
    CACCACCAGC GTCAGTTGGA AAACATCTGG CAACATCGCA AAGTTCGTCT GCATCAACGC
    CTACAGCTCT GTGTTTTTCA GCAAGATGTT CAGCAGGTCT TGGATTGGAT AGAGAACCAT
    GGTGAAGCCT TTCTCAGCAA ACACACTGGG GTAGGCAAGT CTCTGCACAG GGCCAGAGCC
    TTGCAAAAGC GACATGAAGA CTTTGAAGAA GTCGCTCAGA ATACATACAC CAATGCTGAT
    AAGCTGCTGG AAGCTGCAGA ACAGTTGGCC CAGACAGGAG AGTGCGATCC AGAGGAAATA
    TACCAGGCTG CTCATCAGCT TGAGGATCGG ATTCAGGATT TTGTAAGACG TGTAGAACAA
    AGAAAAGTTC TCTTGGACAT GTCTGTATCA TTCCACTCGC ATGTTAAAGA GCTATGGACA
    TGGCTGGAGG ACCTACAAAA GGAACTGCTG GATGATGTCT ATGCGGAGTC AGTAGAAGCT
    GTACAGGACT TGATCAAACG GTTTGGACAA CAGCAGCAGA CAACCCTCCA GTTTACAGTA
    AATGTCATCA AAGAAGGGGA GGATCTAATT CAGCAACTAA GAGATTCTGC TATATCCAGC
    AACAAAACTC CTCACAACAG CTCAATCAAT CACATTGAGA CTGTCCTTCA ACAACTTGAT
    GAGGCCCAAT CACAGATGGA GGAGCTTTTC CAAGAGAGAA AAATCAAACT TGAGCTATTC
    TTGCAGCTTC GCATCTTTGA GAGAGATGCC ATTGATATCA TCTCAGACTT GGAATCATGG
    AATGATGAAC TTTCTCAGCA AATGAATGAT TTTGACACAG AGGACCTCAC ACTAGCCGAG
    CAAAGGCTAC AGCATCACGC AGACAAAGCT CTGACAATGA ACAACCTGAC ATTTGATGTC
    ATCCACCAAG GACAAGAGTT ACTACAATAT GTGAATGAAG TTCAGAACTC AGGGGTTGAG
    CTACTCTGTG ATAGAGATGT AGATATGGCT ACTAGAGTTC AGGACCTGTT GGAATTCCTC
    CATGAGAAAC AACAGGAGTT GGATCTAGCA GCTGAGCAGC ACCGAAAACA TCTAGAACAA
    TGTGTGCAAC TACGACATTT GCAAGCTGAA GTCAAACAGG TACTCGGCTG GATACGGAAT
    GGAGAGTCTA TGTTAAATGC CGGGCTTATC ACTGCCAGTT CATTGCAAGA AGCAGAGCAA
    CTTCAAAGGG AACATGAGCA GTTCCAGCAT GCAATAGAAA AAACCCACCA GAGTGCTCTG
    CAGGTGCAGC AGAAGGCAGA GGCCATGCTT CAAGCAAACC ATTACGACAT GGATATGATC
    CGGGACTGTG CTGAAAAAGT GGCCTCTCAT TGGCAACAGT TGATGCTTAA AATGGAAGAC
    CGTCTTAAGC TTGTCAATGC CTCTGTGGCC TTTTATAAAA CCTCAGAGCA GGTATGCAGT
    GTTCTAGAGA GCCTAGAGCA GGAGTACAAG CGGGAGGAAG ATTGGTGTGG CGGAGCTGAT
    AAACTTGGGC CTAACTCAGA GACTGACCAT GTTACTCCTA TGATCAGCAA ACACCTTGAA
    CAAAAGGAAG CCTTTCTTAA GGCCTGCACT TTGGCTCGCA GAAATGCTGA TGTCTTTCTG
    AAATACTTGC ACAGGAATAG TGTCAATATG CCTGGAATGG TGACACATGT AAAGGCTCCT
    GAACAGCAAG TAAAAAATAT TTTAAATGAG CTGTTTCAGA GGGAAAATAG AGTTCTTCAT
    TATTGGACCT TGCGGAAAAG GAGACTAGAC CATTGCCAGC AGTATGTAGT ATTTGAAAGA
    AGTGCCAAAC AGGCTCTGGA GTGGATCCAA GATAATGGAG AATTTTACAT TTCCACACAC
    ACCTCCACTG GCTCCACAAT TCAGCATACA CAAGAACTCT TAAAAGAGCA TGAGGAGTTT
    CAGATCACAG CAAAGCAAAC CAAAGAGAGG GTAAAGCTGT TGATACAGCT GGCTGATGGC
    TTTTGTGAAA AAGGGCATGC TCATGCTGCA GAAATTAAAA AATGTGTTAC AGCGGTGGAC
    AAGAGGTACA GAGACTTCTC GCTGCGTATG GAAAAGTACA GAACCAGCTT AGAGAAGGCT
    TTGGGCATTT CTTCTGATTC CAAGTCGAGT AAAAGCCTTC AGTTGGATAT TATTCCAGCC
    ACTGCCCCTG GTTCCGAAGT CAAGCTTCGT GATGCAACAC ATGAACTCAA TGAGGAGAAA
    AGAAAATCTG CTCGTCGAAA AGAATTCATC ATGGCAGAAC TTATACAAAC TGAAAAAGCA
    TATGTTCGTG ATCTTCGGGA ATGCATGGAT ACATATCTGT GGGAAATGAC AAGCGGAGTT
    GAAGAGATCC CACCAGGCAT TGTAAATAAA GAACACATTA TCTTTGGAAA CATGCAGGAA
    ATATATGAAT TTCATAACAA CATATTCTTA AAGGAGCTTG AGAAGTATGA GCAGCTACCT
    GAAGATGTTG GACATTGTTT TGTCACATGG GCTGACAAAT TTCAGATGTA TGTGTCCTAC
    TGTAAGAATA AGCCTGATTC AACACAACTT ATCTTGGAAC AAGCAGGAAC ATATTTTGAT
    GAAATTCAGC AGCGCCATGA GCTGGTCAAC TCCATCTCTT CTTATCTTAT CAAACCTGTG
    CAAAGGATAA CAAAATATCA GCTTCTTTTA AAAGAGCTGC TGACATGCTG CGAAGAAGGT
    AAAGGAGAGA TTAAAGATGG GCTGGAAGTG ATGCTAAGTG TACCAAAAAG AGCCAACGAT
    GCAATGCATC TCAGCATGCT TGAAGGGTTT GATGAGAACA TTGAATCTCA GGGAGAACTT
    ATTCTGCAAG AGTCTTTTCA AGTGTGGGAT CCTAAAACCT TAATAAGAAA GGGGCGTGAG
    AGACATCTCT TCTTGTTTGA AATGTCTTTG GTCTTCAGCA AAGAAGTGAA AGATTCAAGT
    GGAAGGAGCA AATATATTTA CAAATCAAAA TTATTTACCT CAGAGCTTGG AGTCACAGAA
    CATGTAGAAG GTGATCCTTG CAAATTTGCA CTTTGGGTAG GCAGAACGCC AACATCTGAT
    AACAAAATAG TCCTTAAGGG CTCAAGTATT GAGAACAAGC AAGATTGGAT CAAGCATATA
    AGAGAAGTGA TCCAAGACCG AACCATTCAC CTGAGAGGCG CATTAAAAGA GCCTATTCAT
    ATACCTAAAA CAGCTGCTGC TACAAAACAG AAACCTGCCA AAAGAGATGG AGAAGACCTG
    GACAGTCAAG GAGATGGCAG CAGCCAACCT GATACAATAT CTATTGCTTC ACGAACTTCA
    CAGAACACAT TAGACAGTGA TAAGCTTTCT GGTGGATGTG AGCTGACAGT AGTCATCCAT
    GACTTTACTG CCTGTAATAG CAATGAGCTG ACAATTAAAC GAGGGCAGAC TGTGGAGGTT
    CTAGAGCGGC ATCACGATCG ACCTGACTGG TGTTTAGTTC GGACTACTGA CCGGTCACCT
    GCAGCAGAGG GCTTGGTGCC TAGTAGTTCA CTGTGTATTG CACATTCTAG AAGTAGCATG
    GAAATGGAAG GCATATTTAA CCACAAAGAT TCCCTCTCTG TCTCCAGTAA TGATGCCAGT
    CCTCCTGCTT CAGTAATTTC CCTTCAGCCT CACATGATTG GTGCTCAAAG CTCTCCTGGC
    CCAAAGCGAC CTGGCAATAC TTTGAGAAAA TGGCTCACCA GCCCTGTCAG GAGACTTAGC
    AGTGGCAAAG CTGATGGACA TGTGAAAAAA TTGGCTCATA AACACAAAAA AAGCAGGGAG
    GTTCGTAAAA ACACAGACTC AGGTTCCCAA AAGGACTCTG ATGACAGTGC AGCAACACCT
    CAAGATGAAA CTATCGAGGA GAGAGTGCGC AATGAAGGAC TTAGCAGTGG TACGCTTTCT
    AAATCCTCCT CGTCTGGCAT GCAAAGCTGT GGAGAAGAAG AGGGCGAGGA AGGACCTGAT
    GCAGTTCCAT TGCCTCCACC CATGGCTATT CAGCAGCACA GCCTTCTTCA GCCTGACTCA
    CAGGATGACA AGACATCATC TCGGTTGCTG GTGCGACCTA CTAGCTCAGA GACACCTAGT
    GCTGCAGAGC TTGTTAGTGC TATTGAAGAA CTTGTAAAGA GTAAAATGGC TTTGGAAGAC
    CGGCCAAGTT CACTTCAAGT AGACCAGGGA GACAGTAGTA GCCCATCATT CAACCCTTCA
    GATACATCTC TGCTTTCCTC CTCTTCTCCA ATTGATGAAA TGGAAGAAAA AAAATCAAGC
    TCTTTAAAAA GAAGGCACTA TGTATTACAA GAGCTAGTAG AAACTGAACG CGATTATGTT
    CGGGACCTTG GCTATGTGGT TGAGGGTTAT ATGGCACTTA TGAAGGAGGA TGGAGTTCCA
    GATGATATGA AAGGAAAAGA CAAGATTGTT TTTGGCAACT TACACCAGAT CTACGAGTGG
    CACAGAGACT TTTTTTTGGG TGAACTGGAA AGGTGCCTGG ATGAGCCTGA AAGATTGGCA
    TCCCTATTTG TCAAGCATGA AAGAAGATTG CATATGTACA TAGTTTACTG CCAAAATAAA
    CCAAAGTCTG AACATATTGT TTCAGAATAC ATTGATACCT ATTTTGAGGA CCTGAAACAG
    CGTCTGGGCC ATCGACTTCA GCTTACAGAT CTGTTAATAA AACCCATACA AAGAATAATG
    AAATATCAGC TGCTATTAAA GGATTTTCTC AAGTACTCTA AGAAGGCTTG CATAGATGTA
    GCAGAACTTG AGAGAGCTGT GGAAGTCATG TGTATCGTAC CGAAGCGGTG TAATGACATG
    ATGAATGTTG GTCGTTTGCA AGGATTTGAT GGCAAAATAG TTGCCCAAGG AAAGCTGCTA
    CTGCAAGACA CATTCCTTGT GACTGAGCAG GACGCTGGTC TTCTGCCCCG ATGCAAAGAA
    AGGCGGGTCT TCCTTTTCGA ACAAATTGTC ATCTTTAGTG AGCCCTTAGA TAAAAAGAAA
    GGATACTCTA CACCTGGATT TCTTTTTAAA AATAATATCA AAGTGAGCTG CCTTAGTTTA
    GAGGAGTGTG TTGATAGTGA TCCATGCAAG TTTGCACTAT TGTCAAGGTC TGCTGACTCT
    ACAGAGATGT TTGTTTTGCA TTCTTCATCG CCTGGAGTGA GACAGTTATG GATTCATGAA
    ATCAACCAAA TTTTGGAAAA CCAGCGCAAT TTTTTAAATG CCTTGACATC ACCAATTGAA
    TACCACAGAA ATCACACTGG TGGAAGCAGT AGTGGCAACG TCACCTTTAC CAGCAGTCCC
    AGCAATTGTA GTGGGCTGGG TGGCTCCAGC CGAAGCAGGC CCTCTCGGAT TCCCCAGCCT
    GTTAGACACC ACTCTCCAGT CCTGGTCTCC TCTGCAGCTT CGGCACAGTC AGAGGCAGAC
    AAGATGTCAG GTATGTCAGC TCGCCGTGCA CCAGTTGTAC CTCTCAACCT CTCCTTAGAC
    AATAACCCCA GCCAGAAAGG TAGAGTAACT GCTGCTCCAG AGACAGGCGG GCGAGATAAA
    GAATCCAATT CAGTGCCAAA GATGAAAATG CTTGATGGCC CCAGGAAATC AGCTAGTGGC
    ATTCCAACCT TAGCTGATGG TGCACAGAAA GAATTGCGTG GGAGCACAGA AGACAGTCGA
    ATTAGAAGCA CTATGGGCAC TCTAACCTTA GGAAAGCCAA GAAATGGTTC TGCCTCACCC
    CTCAACTCTC CTGTCACAAT TAATTTTCCA TCGTCTTTTG GTAAGGAATC AGGGCCACCA
    AGCAGTCCCC TTCAGAAAGG CTCTTTTTGG AGCTCAATTC CAGGATCTCC AGCCAGCCGA
    CCTGGCTCCT TCACTTTCCC TGGAGACAGT GACTCTCTCC AGCGACAAGT TCATCGGCAC
    TCCACACACA GCAAGGATAC AGACCGCATG AGCACCTGCT CCTCGACTAG TGAGCAGTCC
    GTTCACTCCA CTCAGAGTAA TGGGGTAAGG ACAGAGAGTG AAAGTAGCAG CAGTAGCAAT
    ATCTCCACCA TGCTGGTTAC GCACGATTAT ACAGCGGTGA AGGAGGATGA GATCAATGTC
    TACCAGGGAG AGGTTGTACA AATTCTGGCA AGTAACCAAC AGAACATGTT TCTGGTGTTC
    AGAGCTGCCA CTGATGAGTG CCCTGCAGCT GAGGGCTGGA TTCCAAGCTA TGTTCTGGGT
    CACACAAGTG CAATCATCAC TGATATGACT GATGCAACTA TGAAGAAGTC CTCTTCTTGG
    CATACAGCCC TTAGAATAAG AAAGAAATCT GAGAAGAGGG AAAAAGATGG CAGCAGAGAC
    ACAAAACTAG AGAATGGTTA TCGCAAATCA AGAGAAGGAC TAGCTAATAA GGTATCAGTT
    AAGCTCCTGA ATCCAAACTA CATTTATGAT GCTCCTCCAG AGTTTATTGT ACCACTGAAC
    GAGGTTACAT GTGACACAGG AGAGACTGTG ATTATTCGAT GCAAAGTTTG TGGTCGCCCC
    AAGGCATCCA TTACATGGAA AGGTCCCGAC CATAATACTT TGAGCAACGA TGGACACTAC
    AGTATATCCT ACAGTGATCT TGGAGAAGCA ACTCTAAAAA TAGTTGGTGT GACCACTGAG
    GATGATGGTA TATACACCTG CATTGCAACA AATGACTTGG GTTCAGTATC CGCTTCCTCC
    AATCTTAGAG TGTTAGGTTC ATGGAGTGAT GGACTGAGAG TAATCTGGAA AGAAAACTTC
    GACATTGCTT ATAGTGAAGT GGCTGAACTT GGCAGGGGCA GGTTTTCTGT CGTGAAAAAA
    TGTGAACAAA GAGTTACAAG ACGCATAGTA GCCACCAAAT TTGTTAACAA GAAGTTGATG
    AAAAGAGACC AGGTTACCCA TGAACTTGGA ATTCTGCAGA GCCTTCAGCA CCCACAACTG
    ATCAGCCTTC TTGACACTTT TGAGACACCA GCAAGCTATG TCTTAGTACT TGAGATAGCT
    GATCAGGGAT GTTTGTTAGA ATATGTGGTA CAGTGGGGAA ACCTTACAGA AGAAAAGATC
    ACCTCATATA TGAGAGAGGT GCTGGAGGCC CTGCAGTATC TTCATAATTG CAGAATAGCA
    CATCTGGACC TAAAGCCTGA GAACATTCTT GTGGATCAGA GCCTAACCAA ACCAACTATA
    AAACTGTCAG ACTTTGGCGA TGCTGTTCAG CTTAATACCA CTTACTACAT TCACCAATTG
    TTGGGCAGTG CAGAGTTTGC CGCTCCAGAG ATCATCCTTG GGAATCCTGT CTCCCTTACC
    TCTGATATAT GGAGTATTGG AGTTCTTACT TATGTCCTTC TCAGTGGCGT CTCTCCTTTC
    CTTGATGAAA GTGTTGAAGA GACATGCCTG AACATTTGCC GACTAGACTT TAGTTTTCCC
    GATGACTACT TTAAGGGAGT AAGTCAGAAG GCCAAAGAAT TTGTATGTTT CTTACTGCAG
    GGTGACCAGA CAAAGCGCCC TTCTGCTGCT GTATGTCTAA AGGAACAGTG GTTGCAGCCA
    GGCCAGCACA AACGTAGTGG CACATTAGAC ATGTCAAGAC TGACCTCGTT TATTGAACGC
    CGTAAACACC AGAATGATGT TCGACCCATT GGTAGCATTA AAAACTTCCT TCAGAGTCGC
    CTTTTACCCA GAATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32254
    Clone ID Related Accession (Same CDS sequence) XM_012964354.2 , XM_012964354.3
    Accession Version XM_012964354.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9246bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product triple functional domain protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964354.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
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    1621
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    1801
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    1981
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    8401
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    8641
    8701
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    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGAGCACAT CCAGCACCGC AAGCTCCGGT AGCGCAAATA CCGGTACCGG CAATGCCGTT 
    GGAAGCAGCG GAGGCAACAC CGGGGAGGGG GCGGAGGAGA CGGCCAAGGA CTCTGCAGAC
    ATAGCTGCCT TTTACAGATC CGGTTTTCGT AGAAATGATG AATTGAAAGC AATGGAGGTG
    TTGCCAATTT TAAAAGAAAA AGTGGCATAT CTATCAGGTG GCAGAGATAA ACGAGGTGGT
    CCTGTATTAA CATTTCCAGC ACGCAGCAAC CATGATCGAA TACGACAAGA GGATCTCCGA
    AGACTGATTT CATATCTTGC TTGTATTCCT AGTGAGGAGG TGTGCAAGCG AGGCTTCACA
    GTGATTGTAG ATATGCGTGG TTCCAAGTGG GATTCTATTA AACCCTTGCT GAAAATTTTA
    CAAGAATCCT TTCCATGCTG TATTCATGTT GCACTAATTA TCAAACCAGA TAACTTCTGG
    CAGAAGCAAA GAACTAATTT TGGAAGTTCT AAGTTTGAAT TTGAGACAAG TATGGTGTCT
    TTAGAAGGCC TTACCAAAGT AGTTGACCCT TCCCAGCTAA CTCCAGAATT TGAGGGTTGC
    TTGGAATACA ACCATGAAGA GTGGATAGAG ATCAGAGTAG CATTTGAGGA TTTTATTAAT
    AATGCAACTC ATATGCTGTC TCGATTAGAG GAATTACAGG AGATGCTTGC AATTAAAGAG
    TTGCCTCAGG ATTTAGAGGG GGCACGCAAT ATGATTGAAG AACATTCTCA AATGAAAAAA
    AGGGTTATAA AGGCTCCTAT TGAAGATCTT GATTTAGAAG GGCAAAAGCT TTTACAAACA
    ATACAAAGCA GTGATAGCTT TCCTAAGAAG AACTCTAGTT CTGGAAATGC AGACCTTCAA
    AATCTTCTTC CCAAAGTTTC CACAATGTTA GACAGGCTTC ATTCAACACG ACAACATCTT
    CATCAGATGT GGCACATGCG AAAAGTAAAA CTGGACCAGT GCTTTCAGCT AAGGTTGTTT
    GAGCAGGATG CTGAGAAGAT GTTTGACTGG ATTACACACA ACAAAGGTCT GTTTCTGACA
    AGTTATACTG ACATTGGCAC CCGTAATAAT CATGCTATGG AACTTCAGAC ACAGCACAAT
    CATTTTGCTA TGAACTGCAT GAATGTCTAT GTAAACATTA GCCGAATAAT GTCTGTGGCA
    AATCGACTAG TAGAATCTGG ACACTATGCC TCACAGCAAA TTAAGCAGAT AGCTAACCAG
    TTGGAGCAAG AGTGGAAGGC TTTTGCCGCA GCCCTTGATG AACGTAGTAC GTTGCTTGAC
    ATGTCTGCAA TGTTCCATCA AAAAGCGGAG CAGTACATGG GCAATGTGGA TTCATGGTGC
    AAAGCTTGTG GGGAAGTGGA TCTTCCATCA GAGCTGCAGG ATCTAGAAGA TGCCATTCAT
    CACCATCAGG GAATATATGA GCATATCACT TTAGCATATT CAGAGGTTAG CCAGGATGGT
    AAATCTTTAT TAGACAAACT CCAGAGACCA TTGACTCCTG GTAGTTCTGA CTCACTGACA
    GCTTCTGCCA ACTATTCAAA GGCTGTGCAT CACGTCTTAG ATGCAATACA TGAAGTCTTG
    CACCACCAGC GTCAGTTGGA AAACATCTGG CAACATCGCA AAGTTCGTCT GCATCAACGC
    CTACAGCTCT GTGTTTTTCA GCAAGATGTT CAGCAGGTCT TGGATTGGAT AGAGAACCAT
    GGTGAAGCCT TTCTCAGCAA ACACACTGGG GTAGGCAAGT CTCTGCACAG GGCCAGAGCC
    TTGCAAAAGC GACATGAAGA CTTTGAAGAA GTCGCTCAGA ATACATACAC CAATGCTGAT
    AAGCTGCTGG AAGCTGCAGA ACAGTTGGCC CAGACAGGAG AGTGCGATCC AGAGGAAATA
    TACCAGGCTG CTCATCAGCT TGAGGATCGG ATTCAGGATT TTGTAAGACG TGTAGAACAA
    AGAAAAGTTC TCTTGGACAT GTCTGTATCA TTCCACTCGC ATGTTAAAGA GCTATGGACA
    TGGCTGGAGG ACCTACAAAA GGAACTGCTG GATGATGTCT ATGCGGAGTC AGTAGAAGCT
    GTACAGGACT TGATCAAACG GTTTGGACAA CAGCAGCAGA CAACCCTCCA GTTTACAGTA
    AATGTCATCA AAGAAGGGGA GGATCTAATT CAGCAACTAA GAGATTCTGC TATATCCAGC
    AACAAAACTC CTCACAACAG CTCAATCAAT CACATTGAGA CTGTCCTTCA ACAACTTGAT
    GAGGCCCAAT CACAGATGGA GGAGCTTTTC CAAGAGAGAA AAATCAAACT TGAGCTATTC
    TTGCAGCTTC GCATCTTTGA GAGAGATGCC ATTGATATCA TCTCAGACTT GGAATCATGG
    AATGATGAAC TTTCTCAGCA AATGAATGAT TTTGACACAG AGGACCTCAC ACTAGCCGAG
    CAAAGGCTAC AGCATCACGC AGACAAAGCT CTGACAATGA ACAACCTGAC ATTTGATGTC
    ATCCACCAAG GACAAGAGTT ACTACAATAT GTGAATGAAG TTCAGAACTC AGGGGTTGAG
    CTACTCTGTG ATAGAGATGT AGATATGGCT ACTAGAGTTC AGGACCTGTT GGAATTCCTC
    CATGAGAAAC AACAGGAGTT GGATCTAGCA GCTGAGCAGC ACCGAAAACA TCTAGAACAA
    TGTGTGCAAC TACGACATTT GCAAGCTGAA GTCAAACAGG TACTCGGCTG GATACGGAAT
    GGAGAGTCTA TGTTAAATGC CGGGCTTATC ACTGCCAGTT CATTGCAAGA AGCAGAGCAA
    CTTCAAAGGG AACATGAGCA GTTCCAGCAT GCAATAGAAA AAACCCACCA GAGTGCTCTG
    CAGGTGCAGC AGAAGGCAGA GGCCATGCTT CAAGCAAACC ATTACGACAT GGATATGATC
    CGGGACTGTG CTGAAAAAGT GGCCTCTCAT TGGCAACAGT TGATGCTTAA AATGGAAGAC
    CGTCTTAAGC TTGTCAATGC CTCTGTGGCC TTTTATAAAA CCTCAGAGCA GGTATGCAGT
    GTTCTAGAGA GCCTAGAGCA GGAGTACAAG CGGGAGGAAG ATTGGTGTGG CGGAGCTGAT
    AAACTTGGGC CTAACTCAGA GACTGACCAT GTTACTCCTA TGATCAGCAA ACACCTTGAA
    CAAAAGGAAG CCTTTCTTAA GGCCTGCACT TTGGCTCGCA GAAATGCTGA TGTCTTTCTG
    AAATACTTGC ACAGGAATAG TGTCAATATG CCTGGAATGG TGACACATGT AAAGGCTCCT
    GAACAGCAAG TAAAAAATAT TTTAAATGAG CTGTTTCAGA GGGAAAATAG AGTTCTTCAT
    TATTGGACCT TGCGGAAAAG GAGACTAGAC CATTGCCAGC AGTATGTAGT ATTTGAAAGA
    AGTGCCAAAC AGGCTCTGGA GTGGATCCAA GATAATGGAG AATTTTACAT TTCCACACAC
    ACCTCCACTG GCTCCACAAT TCAGCATACA CAAGAACTCT TAAAAGAGCA TGAGGAGTTT
    CAGATCACAG CAAAGCAAAC CAAAGAGAGG GTAAAGCTGT TGATACAGCT GGCTGATGGC
    TTTTGTGAAA AAGGGCATGC TCATGCTGCA GAAATTAAAA AATGTGTTAC AGCGGTGGAC
    AAGAGGTACA GAGACTTCTC GCTGCGTATG GAAAAGTACA GAACCAGCTT AGAGAAGGCT
    TTGGGCATTT CTTCTGATTC CAAGTCGAGT AAAAGCCTTC AGTTGGATAT TATTCCAGCC
    ACTGCCCCTG GTTCCGAAGT CAAGCTTCGT GATGCAACAC ATGAACTCAA TGAGGAGAAA
    AGAAAATCTG CTCGTCGAAA AGAATTCATC ATGGCAGAAC TTATACAAAC TGAAAAAGCA
    TATGTTCGTG ATCTTCGGGA ATGCATGGAT ACATATCTGT GGGAAATGAC AAGCGGAGTT
    GAAGAGATCC CACCAGGCAT TGTAAATAAA GAACACATTA TCTTTGGAAA CATGCAGGAA
    ATATATGAAT TTCATAACAA CATATTCTTA AAGGAGCTTG AGAAGTATGA GCAGCTACCT
    GAAGATGTTG GACATTGTTT TGTCACATGG GCTGACAAAT TTCAGATGTA TGTGTCCTAC
    TGTAAGAATA AGCCTGATTC AACACAACTT ATCTTGGAAC AAGCAGGAAC ATATTTTGAT
    GAAATTCAGC AGCGCCATGA GCTGGTCAAC TCCATCTCTT CTTATCTTAT CAAACCTGTG
    CAAAGGATAA CAAAATATCA GCTTCTTTTA AAAGAGCTGC TGACATGCTG CGAAGAAGGT
    AAAGGAGAGA TTAAAGATGG GCTGGAAGTG ATGCTAAGTG TACCAAAAAG AGCCAACGAT
    GCAATGCATC TCAGCATGCT TGAAGGGTTT GATGAGAACA TTGAATCTCA GGGAGAACTT
    ATTCTGCAAG AGTCTTTTCA AGTGTGGGAT CCTAAAACCT TAATAAGAAA GGGGCGTGAG
    AGACATCTCT TCTTGTTTGA AATGTCTTTG GTCTTCAGCA AAGAAGTGAA AGATTCAAGT
    GGAAGGAGCA AATATATTTA CAAATCAAAA TTATTTACCT CAGAGCTTGG AGTCACAGAA
    CATGTAGAAG GTGATCCTTG CAAATTTGCA CTTTGGGTAG GCAGAACGCC AACATCTGAT
    AACAAAATAG TCCTTAAGGG CTCAAGTATT GAGAACAAGC AAGATTGGAT CAAGCATATA
    AGAGAAGTGA TCCAAGACCG AACCATTCAC CTGAGAGGCG CATTAAAAGA GCCTATTCAT
    ATACCTAAAA CAGCTGCTGC TACAAAACAG AAACCTGCCA AAAGAGATGG AGAAGACCTG
    GACAGTCAAG GAGATGGCAG CAGCCAACCT GATACAATAT CTATTGCTTC ACGAACTTCA
    CAGAACACAT TAGACAGTGA TAAGCTTTCT GGTGGATGTG AGCTGACAGT AGTCATCCAT
    GACTTTACTG CCTGTAATAG CAATGAGCTG ACAATTAAAC GAGGGCAGAC TGTGGAGGTT
    CTAGAGCGGC ATCACGATCG ACCTGACTGG TGTTTAGTTC GGACTACTGA CCGGTCACCT
    GCAGCAGAGG GCTTGGTGCC TAGTAGTTCA CTGTGTATTG CACATTCTAG AAGTAGCATG
    GAAATGGAAG GCATATTTAA CCACAAAGAT TCCCTCTCTG TCTCCAGTAA TGATGCCAGT
    CCTCCTGCTT CAGTAATTTC CCTTCAGCCT CACATGATTG GTGCTCAAAG CTCTCCTGGC
    CCAAAGCGAC CTGGCAATAC TTTGAGAAAA TGGCTCACCA GCCCTGTCAG GAGACTTAGC
    AGTGGCAAAG CTGATGGACA TGTGAAAAAA TTGGCTCATA AACACAAAAA AAGCAGGGAG
    GTTCGTAAAA ACACAGACTC AGGTTCCCAA AAGGACTCTG ATGACAGTGC AGCAACACCT
    CAAGATGAAA CTATCGAGGA GAGAGTGCGC AATGAAGGAC TTAGCAGTGG TACGCTTTCT
    AAATCCTCCT CGTCTGGCAT GCAAAGCTGT GGAGAAGAAG AGGGCGAGGA AGGACCTGAT
    GCAGTTCCAT TGCCTCCACC CATGGCTATT CAGCAGCACA GCCTTCTTCA GCCTGACTCA
    CAGGATGACA AGACATCATC TCGGTTGCTG GTGCGACCTA CTAGCTCAGA GACACCTAGT
    GCTGCAGAGC TTGTTAGTGC TATTGAAGAA CTTGTAAAGA GTAAAATGGC TTTGGAAGAC
    CGGCCAAGTT CACTTCAAGT AGACCAGGGA GACAGTAGTA GCCCATCATT CAACCCTTCA
    GATACATCTC TGCTTTCCTC CTCTTCTCCA ATTGATGAAA TGGAAGAAAA AAAATCAAGC
    TCTTTAAAAA GAAGGCACTA TGTATTACAA GAGCTAGTAG AAACTGAACG CGATTATGTT
    CGGGACCTTG GCTATGTGGT TGAGGGTTAT ATGGCACTTA TGAAGGAGGA TGGAGTTCCA
    GATGATATGA AAGGAAAAGA CAAGATTGTT TTTGGCAACT TACACCAGAT CTACGAGTGG
    CACAGAGACT TTTTTTTGGG TGAACTGGAA AGGTGCCTGG ATGAGCCTGA AAGATTGGCA
    TCCCTATTTG TCAAGCATGA AAGAAGATTG CATATGTACA TAGTTTACTG CCAAAATAAA
    CCAAAGTCTG AACATATTGT TTCAGAATAC ATTGATACCT ATTTTGAGGA CCTGAAACAG
    CGTCTGGGCC ATCGACTTCA GCTTACAGAT CTGTTAATAA AACCCATACA AAGAATAATG
    AAATATCAGC TGCTATTAAA GGATTTTCTC AAGTACTCTA AGAAGGCTTG CATAGATGTA
    GCAGAACTTG AGAGAGCTGT GGAAGTCATG TGTATCGTAC CGAAGCGGTG TAATGACATG
    ATGAATGTTG GTCGTTTGCA AGGATTTGAT GGCAAAATAG TTGCCCAAGG AAAGCTGCTA
    CTGCAAGACA CATTCCTTGT GACTGAGCAG GACGCTGGTC TTCTGCCCCG ATGCAAAGAA
    AGGCGGGTCT TCCTTTTCGA ACAAATTGTC ATCTTTAGTG AGCCCTTAGA TAAAAAGAAA
    GGATACTCTA CACCTGGATT TCTTTTTAAA AATAATATCA AAGTGAGCTG CCTTAGTTTA
    GAGGAGTGTG TTGATAGTGA TCCATGCAAG TTTGCACTAT TGTCAAGGTC TGCTGACTCT
    ACAGAGATGT TTGTTTTGCA TTCTTCATCG CCTGGAGTGA GACAGTTATG GATTCATGAA
    ATCAACCAAA TTTTGGAAAA CCAGCGCAAT TTTTTAAATG CCTTGACATC ACCAATTGAA
    TACCACAGAA ATCACACTGG TGGAAGCAGT AGTGGCAACG TCACCTTTAC CAGCAGTCCC
    AGCAATTGTA GTGGGCTGGG TGGCTCCAGC CGAAGCAGGC CCTCTCGGAT TCCCCAGCCT
    GTTAGACACC ACTCTCCAGT CCTGGTCTCC TCTGCAGCTT CGGCACAGTC AGAGGCAGAC
    AAGATGTCAG GTATGTCAGC TCGCCGTGCA CCAGTTGTAC CTCTCAACCT CTCCTTAGAC
    AATAACCCCA GCCAGAAAGG TAGAGTAACT GCTGCTCCAG AGACAGGCGG GCGAGATAAA
    GAATCCAATT CAGTGCCAAA GATGAAAATG CTTGATGGCC CCAGGAAATC AGCTAGTGGC
    ATTCCAACCT TAGCTGATGG TGCACAGAAA GAATTGCGTG GGAGCACAGA AGACAGTCGA
    ATTAGAAGCA CTATGGGCAC TCTAACCTTA GGAAAGCCAA GAAATGGTTC TGCCTCACCC
    CTCAACTCTC CTGTCACAAT TAATTTTCCA TCGTCTTTTG GTAAGGAATC AGGGCCACCA
    AGCAGTCCCC TTCAGAAAGG CTCTTTTTGG AGCTCAATTC CAGGATCTCC AGCCAGCCGA
    CCTGGCTCCT TCACTTTCCC TGGAGACAGT GACTCTCTCC AGCGACAAGT TCATCGGCAC
    TCCACACACA GCAAGGATAC AGACCGCATG AGCACCTGCT CCTCGACTAG TGAGCAGTCC
    GTTCACTCCA CTCAGAGTAA TGGGAGTGAA AGTAGCAGCA GTAGCAATAT CTCCACCATG
    CTGGTTACGC ACGATTATAC AGCGGTGAAG GAGGATGAGA TCAATGTCTA CCAGGGAGAG
    GTTGTACAAA TTCTGGCAAG TAACCAACAG AACATGTTTC TGGTGTTCAG AGCTGCCACT
    GATGAGTGCC CTGCAGCTGA GGGCTGGATT CCAAGCTATG TTCTGGGTCA CACAAGTGCA
    ATCATCACTG ATATGACTGA TGCAACTATG AAGAAGTCCT CTTCTTGGCA TACAGCCCTT
    AGAATAAGAA AGAAATCTGA GAAGAGGGAA AAAGATGGCA GCAGAGACAC AAAACTAGAG
    AATGGTTATC GCAAATCAAG AGAAGGACTA GCTAATAAGG TATCAGTTAA GCTCCTGAAT
    CCAAACTACA TTTATGATGC TCCTCCAGAG TTTATTGTAC CACTGAACGA GGTTACATGT
    GACACAGGAG AGACTGTGAT TATTCGATGC AAAGTTTGTG GTCGCCCCAA GGCATCCATT
    ACATGGAAAG GTCCCGACCA TAATACTTTG AGCAACGATG GACACTACAG TATATCCTAC
    AGTGATCTTG GAGAAGCAAC TCTAAAAATA GTTGGTGTGA CCACTGAGGA TGATGGTATA
    TACACCTGCA TTGCAACAAA TGACTTGGGT TCAGTATCCG CTTCCTCCAA TCTTAGAGTG
    TTAGGTTCAT GGAGTGATGG ACTGAGAGTA ATCTGGAAAG AAAACTTCGA CATTGCTTAT
    AGTGAAGTGG CTGAACTTGG CAGGGGCAGG TTTTCTGTCG TGAAAAAATG TGAACAAAGA
    GTTACAAGAC GCATAGTAGC CACCAAATTT GTTAACAAGA AGTTGATGAA AAGAGACCAG
    GTTACCCATG AACTTGGAAT TCTGCAGAGC CTTCAGCACC CACAACTGAT CAGCCTTCTT
    GACACTTTTG AGACACCAGC AAGCTATGTC TTAGTACTTG AGATAGCTGA TCAGGGATGT
    TTGTTAGAAT ATGTGGTACA GTGGGGAAAC CTTACAGAAG AAAAGATCAC CTCATATATG
    AGAGAGGTGC TGGAGGCCCT GCAGTATCTT CATAATTGCA GAATAGCACA TCTGGACCTA
    AAGCCTGAGA ACATTCTTGT GGATCAGAGC CTAACCAAAC CAACTATAAA ACTGTCAGAC
    TTTGGCGATG CTGTTCAGCT TAATACCACT TACTACATTC ACCAATTGTT GGGCAGTGCA
    GAGTTTGCCG CTCCAGAGAT CATCCTTGGG AATCCTGTCT CCCTTACCTC TGATATATGG
    AGTATTGGAG TTCTTACTTA TGTCCTTCTC AGTGGCGTCT CTCCTTTCCT TGATGAAAGT
    GTTGAAGAGA CATGCCTGAA CATTTGCCGA CTAGACTTTA GTTTTCCCGA TGACTACTTT
    AAGGGAGTAA GTCAGAAGGC CAAAGAATTT GTATGTTTCT TACTGCAGGG TGACCAGACA
    AAGCGCCCTT CTGCTGCTGT ATGTCTAAAG GAACAGTGGT TGCAGCCAGG CCAGCACAAA
    CGTAGTGGCA CATTAGACAT GTCAAGACTG ACCTCGTTTA TTGAACGCCG TAAACACCAG
    AATGATGTTC GACCCATTGG TAGCATTAAA AACTTCCTTC AGAGTCGCCT TTTACCCAGA
    ATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012819808.1
    CDS614..9859
    Translation

    Target ORF information:

    RefSeq Version XM_012964354.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012964354.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGAGCACAT CCAGCACCGC AAGCTCCGGT AGCGCAAATA CCGGTACCGG CAATGCCGTT 
    GGAAGCAGCG GAGGCAACAC CGGGGAGGGG GCGGAGGAGA CGGCCAAGGA CTCTGCAGAC
    ATAGCTGCCT TTTACAGATC CGGTTTTCGT AGAAATGATG AATTGAAAGC AATGGAGGTG
    TTGCCAATTT TAAAAGAAAA AGTGGCATAT CTATCAGGTG GCAGAGATAA ACGAGGTGGT
    CCTGTATTAA CATTTCCAGC ACGCAGCAAC CATGATCGAA TACGACAAGA GGATCTCCGA
    AGACTGATTT CATATCTTGC TTGTATTCCT AGTGAGGAGG TGTGCAAGCG AGGCTTCACA
    GTGATTGTAG ATATGCGTGG TTCCAAGTGG GATTCTATTA AACCCTTGCT GAAAATTTTA
    CAAGAATCCT TTCCATGCTG TATTCATGTT GCACTAATTA TCAAACCAGA TAACTTCTGG
    CAGAAGCAAA GAACTAATTT TGGAAGTTCT AAGTTTGAAT TTGAGACAAG TATGGTGTCT
    TTAGAAGGCC TTACCAAAGT AGTTGACCCT TCCCAGCTAA CTCCAGAATT TGAGGGTTGC
    TTGGAATACA ACCATGAAGA GTGGATAGAG ATCAGAGTAG CATTTGAGGA TTTTATTAAT
    AATGCAACTC ATATGCTGTC TCGATTAGAG GAATTACAGG AGATGCTTGC AATTAAAGAG
    TTGCCTCAGG ATTTAGAGGG GGCACGCAAT ATGATTGAAG AACATTCTCA AATGAAAAAA
    AGGGTTATAA AGGCTCCTAT TGAAGATCTT GATTTAGAAG GGCAAAAGCT TTTACAAACA
    ATACAAAGCA GTGATAGCTT TCCTAAGAAG AACTCTAGTT CTGGAAATGC AGACCTTCAA
    AATCTTCTTC CCAAAGTTTC CACAATGTTA GACAGGCTTC ATTCAACACG ACAACATCTT
    CATCAGATGT GGCACATGCG AAAAGTAAAA CTGGACCAGT GCTTTCAGCT AAGGTTGTTT
    GAGCAGGATG CTGAGAAGAT GTTTGACTGG ATTACACACA ACAAAGGTCT GTTTCTGACA
    AGTTATACTG ACATTGGCAC CCGTAATAAT CATGCTATGG AACTTCAGAC ACAGCACAAT
    CATTTTGCTA TGAACTGCAT GAATGTCTAT GTAAACATTA GCCGAATAAT GTCTGTGGCA
    AATCGACTAG TAGAATCTGG ACACTATGCC TCACAGCAAA TTAAGCAGAT AGCTAACCAG
    TTGGAGCAAG AGTGGAAGGC TTTTGCCGCA GCCCTTGATG AACGTAGTAC GTTGCTTGAC
    ATGTCTGCAA TGTTCCATCA AAAAGCGGAG CAGTACATGG GCAATGTGGA TTCATGGTGC
    AAAGCTTGTG GGGAAGTGGA TCTTCCATCA GAGCTGCAGG ATCTAGAAGA TGCCATTCAT
    CACCATCAGG GAATATATGA GCATATCACT TTAGCATATT CAGAGGTTAG CCAGGATGGT
    AAATCTTTAT TAGACAAACT CCAGAGACCA TTGACTCCTG GTAGTTCTGA CTCACTGACA
    GCTTCTGCCA ACTATTCAAA GGCTGTGCAT CACGTCTTAG ATGCAATACA TGAAGTCTTG
    CACCACCAGC GTCAGTTGGA AAACATCTGG CAACATCGCA AAGTTCGTCT GCATCAACGC
    CTACAGCTCT GTGTTTTTCA GCAAGATGTT CAGCAGGTCT TGGATTGGAT AGAGAACCAT
    GGTGAAGCCT TTCTCAGCAA ACACACTGGG GTAGGCAAGT CTCTGCACAG GGCCAGAGCC
    TTGCAAAAGC GACATGAAGA CTTTGAAGAA GTCGCTCAGA ATACATACAC CAATGCTGAT
    AAGCTGCTGG AAGCTGCAGA ACAGTTGGCC CAGACAGGAG AGTGCGATCC AGAGGAAATA
    TACCAGGCTG CTCATCAGCT TGAGGATCGG ATTCAGGATT TTGTAAGACG TGTAGAACAA
    AGAAAAGTTC TCTTGGACAT GTCTGTATCA TTCCACTCGC ATGTTAAAGA GCTATGGACA
    TGGCTGGAGG ACCTACAAAA GGAACTGCTG GATGATGTCT ATGCGGAGTC AGTAGAAGCT
    GTACAGGACT TGATCAAACG GTTTGGACAA CAGCAGCAGA CAACCCTCCA GTTTACAGTA
    AATGTCATCA AAGAAGGGGA GGATCTAATT CAGCAACTAA GAGATTCTGC TATATCCAGC
    AACAAAACTC CTCACAACAG CTCAATCAAT CACATTGAGA CTGTCCTTCA ACAACTTGAT
    GAGGCCCAAT CACAGATGGA GGAGCTTTTC CAAGAGAGAA AAATCAAACT TGAGCTATTC
    TTGCAGCTTC GCATCTTTGA GAGAGATGCC ATTGATATCA TCTCAGACTT GGAATCATGG
    AATGATGAAC TTTCTCAGCA AATGAATGAT TTTGACACAG AGGACCTCAC ACTAGCCGAG
    CAAAGGCTAC AGCATCACGC AGACAAAGCT CTGACAATGA ACAACCTGAC ATTTGATGTC
    ATCCACCAAG GACAAGAGTT ACTACAATAT GTGAATGAAG TTCAGAACTC AGGGGTTGAG
    CTACTCTGTG ATAGAGATGT AGATATGGCT ACTAGAGTTC AGGACCTGTT GGAATTCCTC
    CATGAGAAAC AACAGGAGTT GGATCTAGCA GCTGAGCAGC ACCGAAAACA TCTAGAACAA
    TGTGTGCAAC TACGACATTT GCAAGCTGAA GTCAAACAGG TACTCGGCTG GATACGGAAT
    GGAGAGTCTA TGTTAAATGC CGGGCTTATC ACTGCCAGTT CATTGCAAGA AGCAGAGCAA
    CTTCAAAGGG AACATGAGCA GTTCCAGCAT GCAATAGAAA AAACCCACCA GAGTGCTCTG
    CAGGTGCAGC AGAAGGCAGA GGCCATGCTT CAAGCAAACC ATTACGACAT GGATATGATC
    CGGGACTGTG CTGAAAAAGT GGCCTCTCAT TGGCAACAGT TGATGCTTAA AATGGAAGAC
    CGTCTTAAGC TTGTCAATGC CTCTGTGGCC TTTTATAAAA CCTCAGAGCA GGTATGCAGT
    GTTCTAGAGA GCCTAGAGCA GGAGTACAAG CGGGAGGAAG ATTGGTGTGG CGGAGCTGAT
    AAACTTGGGC CTAACTCAGA GACTGACCAT GTTACTCCTA TGATCAGCAA ACACCTTGAA
    CAAAAGGAAG CCTTTCTTAA GGCCTGCACT TTGGCTCGCA GAAATGCTGA TGTCTTTCTG
    AAATACTTGC ACAGGAATAG TGTCAATATG CCTGGAATGG TGACACATGT AAAGGCTCCT
    GAACAGCAAG TAAAAAATAT TTTAAATGAG CTGTTTCAGA GGGAAAATAG AGTTCTTCAT
    TATTGGACCT TGCGGAAAAG GAGACTAGAC CATTGCCAGC AGTATGTAGT ATTTGAAAGA
    AGTGCCAAAC AGGCTCTGGA GTGGATCCAA GATAATGGAG AATTTTACAT TTCCACACAC
    ACCTCCACTG GCTCCACAAT TCAGCATACA CAAGAACTCT TAAAAGAGCA TGAGGAGTTT
    CAGATCACAG CAAAGCAAAC CAAAGAGAGG GTAAAGCTGT TGATACAGCT GGCTGATGGC
    TTTTGTGAAA AAGGGCATGC TCATGCTGCA GAAATTAAAA AATGTGTTAC AGCGGTGGAC
    AAGAGGTACA GAGACTTCTC GCTGCGTATG GAAAAGTACA GAACCAGCTT AGAGAAGGCT
    TTGGGCATTT CTTCTGATTC CAAGTCGAGT AAAAGCCTTC AGTTGGATAT TATTCCAGCC
    ACTGCCCCTG GTTCCGAAGT CAAGCTTCGT GATGCAACAC ATGAACTCAA TGAGGAGAAA
    AGAAAATCTG CTCGTCGAAA AGAATTCATC ATGGCAGAAC TTATACAAAC TGAAAAAGCA
    TATGTTCGTG ATCTTCGGGA ATGCATGGAT ACATATCTGT GGGAAATGAC AAGCGGAGTT
    GAAGAGATCC CACCAGGCAT TGTAAATAAA GAACACATTA TCTTTGGAAA CATGCAGGAA
    ATATATGAAT TTCATAACAA CATATTCTTA AAGGAGCTTG AGAAGTATGA GCAGCTACCT
    GAAGATGTTG GACATTGTTT TGTCACATGG GCTGACAAAT TTCAGATGTA TGTGTCCTAC
    TGTAAGAATA AGCCTGATTC AACACAACTT ATCTTGGAAC AAGCAGGAAC ATATTTTGAT
    GAAATTCAGC AGCGCCATGA GCTGGTCAAC TCCATCTCTT CTTATCTTAT CAAACCTGTG
    CAAAGGATAA CAAAATATCA GCTTCTTTTA AAAGAGCTGC TGACATGCTG CGAAGAAGGT
    AAAGGAGAGA TTAAAGATGG GCTGGAAGTG ATGCTAAGTG TACCAAAAAG AGCCAACGAT
    GCAATGCATC TCAGCATGCT TGAAGGGTTT GATGAGAACA TTGAATCTCA GGGAGAACTT
    ATTCTGCAAG AGTCTTTTCA AGTGTGGGAT CCTAAAACCT TAATAAGAAA GGGGCGTGAG
    AGACATCTCT TCTTGTTTGA AATGTCTTTG GTCTTCAGCA AAGAAGTGAA AGATTCAAGT
    GGAAGGAGCA AATATATTTA CAAATCAAAA TTATTTACCT CAGAGCTTGG AGTCACAGAA
    CATGTAGAAG GTGATCCTTG CAAATTTGCA CTTTGGGTAG GCAGAACGCC AACATCTGAT
    AACAAAATAG TCCTTAAGGG CTCAAGTATT GAGAACAAGC AAGATTGGAT CAAGCATATA
    AGAGAAGTGA TCCAAGACCG AACCATTCAC CTGAGAGGCG CATTAAAAGA GCCTATTCAT
    ATACCTAAAA CAGCTGCTGC TACAAAACAG AAACCTGCCA AAAGAGATGG AGAAGACCTG
    GACAGTCAAG GAGATGGCAG CAGCCAACCT GATACAATAT CTATTGCTTC ACGAACTTCA
    CAGAACACAT TAGACAGTGA TAAGCTTTCT GGTGGATGTG AGCTGACAGT AGTCATCCAT
    GACTTTACTG CCTGTAATAG CAATGAGCTG ACAATTAAAC GAGGGCAGAC TGTGGAGGTT
    CTAGAGCGGC ATCACGATCG ACCTGACTGG TGTTTAGTTC GGACTACTGA CCGGTCACCT
    GCAGCAGAGG GCTTGGTGCC TAGTAGTTCA CTGTGTATTG CACATTCTAG AAGTAGCATG
    GAAATGGAAG GCATATTTAA CCACAAAGAT TCCCTCTCTG TCTCCAGTAA TGATGCCAGT
    CCTCCTGCTT CAGTAATTTC CCTTCAGCCT CACATGATTG GTGCTCAAAG CTCTCCTGGC
    CCAAAGCGAC CTGGCAATAC TTTGAGAAAA TGGCTCACCA GCCCTGTCAG GAGACTTAGC
    AGTGGCAAAG CTGATGGACA TGTGAAAAAA TTGGCTCATA AACACAAAAA AAGCAGGGAG
    GTTCGTAAAA ACACAGACTC AGGTTCCCAA AAGGACTCTG ATGACAGTGC AGCAACACCT
    CAAGATGAAA CTATCGAGGA GAGAGTGCGC AATGAAGGAC TTAGCAGTGG TACGCTTTCT
    AAATCCTCCT CGTCTGGCAT GCAAAGCTGT GGAGAAGAAG AGGGCGAGGA AGGACCTGAT
    GCAGTTCCAT TGCCTCCACC CATGGCTATT CAGCAGCACA GCCTTCTTCA GCCTGACTCA
    CAGGATGACA AGACATCATC TCGGTTGCTG GTGCGACCTA CTAGCTCAGA GACACCTAGT
    GCTGCAGAGC TTGTTAGTGC TATTGAAGAA CTTGTAAAGA GTAAAATGGC TTTGGAAGAC
    CGGCCAAGTT CACTTCAAGT AGACCAGGGA GACAGTAGTA GCCCATCATT CAACCCTTCA
    GATACATCTC TGCTTTCCTC CTCTTCTCCA ATTGATGAAA TGGAAGAAAA AAAATCAAGC
    TCTTTAAAAA GAAGGCACTA TGTATTACAA GAGCTAGTAG AAACTGAACG CGATTATGTT
    CGGGACCTTG GCTATGTGGT TGAGGGTTAT ATGGCACTTA TGAAGGAGGA TGGAGTTCCA
    GATGATATGA AAGGAAAAGA CAAGATTGTT TTTGGCAACT TACACCAGAT CTACGAGTGG
    CACAGAGACT TTTTTTTGGG TGAACTGGAA AGGTGCCTGG ATGAGCCTGA AAGATTGGCA
    TCCCTATTTG TCAAGCATGA AAGAAGATTG CATATGTACA TAGTTTACTG CCAAAATAAA
    CCAAAGTCTG AACATATTGT TTCAGAATAC ATTGATACCT ATTTTGAGGA CCTGAAACAG
    CGTCTGGGCC ATCGACTTCA GCTTACAGAT CTGTTAATAA AACCCATACA AAGAATAATG
    AAATATCAGC TGCTATTAAA GGATTTTCTC AAGTACTCTA AGAAGGCTTG CATAGATGTA
    GCAGAACTTG AGAGAGCTGT GGAAGTCATG TGTATCGTAC CGAAGCGGTG TAATGACATG
    ATGAATGTTG GTCGTTTGCA AGGATTTGAT GGCAAAATAG TTGCCCAAGG AAAGCTGCTA
    CTGCAAGACA CATTCCTTGT GACTGAGCAG GACGCTGGTC TTCTGCCCCG ATGCAAAGAA
    AGGCGGGTCT TCCTTTTCGA ACAAATTGTC ATCTTTAGTG AGCCCTTAGA TAAAAAGAAA
    GGATACTCTA CACCTGGATT TCTTTTTAAA AATAATATCA AAGTGAGCTG CCTTAGTTTA
    GAGGAGTGTG TTGATAGTGA TCCATGCAAG TTTGCACTAT TGTCAAGGTC TGCTGACTCT
    ACAGAGATGT TTGTTTTGCA TTCTTCATCG CCTGGAGTGA GACAGTTATG GATTCATGAA
    ATCAACCAAA TTTTGGAAAA CCAGCGCAAT TTTTTAAATG CCTTGACATC ACCAATTGAA
    TACCACAGAA ATCACACTGG TGGAAGCAGT AGTGGCAACG TCACCTTTAC CAGCAGTCCC
    AGCAATTGTA GTGGGCTGGG TGGCTCCAGC CGAAGCAGGC CCTCTCGGAT TCCCCAGCCT
    GTTAGACACC ACTCTCCAGT CCTGGTCTCC TCTGCAGCTT CGGCACAGTC AGAGGCAGAC
    AAGATGTCAG GTATGTCAGC TCGCCGTGCA CCAGTTGTAC CTCTCAACCT CTCCTTAGAC
    AATAACCCCA GCCAGAAAGG TAGAGTAACT GCTGCTCCAG AGACAGGCGG GCGAGATAAA
    GAATCCAATT CAGTGCCAAA GATGAAAATG CTTGATGGCC CCAGGAAATC AGCTAGTGGC
    ATTCCAACCT TAGCTGATGG TGCACAGAAA GAATTGCGTG GGAGCACAGA AGACAGTCGA
    ATTAGAAGCA CTATGGGCAC TCTAACCTTA GGAAAGCCAA GAAATGGTTC TGCCTCACCC
    CTCAACTCTC CTGTCACAAT TAATTTTCCA TCGTCTTTTG GTAAGGAATC AGGGCCACCA
    AGCAGTCCCC TTCAGAAAGG CTCTTTTTGG AGCTCAATTC CAGGATCTCC AGCCAGCCGA
    CCTGGCTCCT TCACTTTCCC TGGAGACAGT GACTCTCTCC AGCGACAAGT TCATCGGCAC
    TCCACACACA GCAAGGATAC AGACCGCATG AGCACCTGCT CCTCGACTAG TGAGCAGTCC
    GTTCACTCCA CTCAGAGTAA TGGGAGTGAA AGTAGCAGCA GTAGCAATAT CTCCACCATG
    CTGGTTACGC ACGATTATAC AGCGGTGAAG GAGGATGAGA TCAATGTCTA CCAGGGAGAG
    GTTGTACAAA TTCTGGCAAG TAACCAACAG AACATGTTTC TGGTGTTCAG AGCTGCCACT
    GATGAGTGCC CTGCAGCTGA GGGCTGGATT CCAAGCTATG TTCTGGGTCA CACAAGTGCA
    ATCATCACTG ATATGACTGA TGCAACTATG AAGAAGTCCT CTTCTTGGCA TACAGCCCTT
    AGAATAAGAA AGAAATCTGA GAAGAGGGAA AAAGATGGCA GCAGAGACAC AAAACTAGAG
    AATGGTTATC GCAAATCAAG AGAAGGACTA GCTAATAAGG TATCAGTTAA GCTCCTGAAT
    CCAAACTACA TTTATGATGC TCCTCCAGAG TTTATTGTAC CACTGAACGA GGTTACATGT
    GACACAGGAG AGACTGTGAT TATTCGATGC AAAGTTTGTG GTCGCCCCAA GGCATCCATT
    ACATGGAAAG GTCCCGACCA TAATACTTTG AGCAACGATG GACACTACAG TATATCCTAC
    AGTGATCTTG GAGAAGCAAC TCTAAAAATA GTTGGTGTGA CCACTGAGGA TGATGGTATA
    TACACCTGCA TTGCAACAAA TGACTTGGGT TCAGTATCCG CTTCCTCCAA TCTTAGAGTG
    TTAGGTTCAT GGAGTGATGG ACTGAGAGTA ATCTGGAAAG AAAACTTCGA CATTGCTTAT
    AGTGAAGTGG CTGAACTTGG CAGGGGCAGG TTTTCTGTCG TGAAAAAATG TGAACAAAGA
    GTTACAAGAC GCATAGTAGC CACCAAATTT GTTAACAAGA AGTTGATGAA AAGAGACCAG
    GTTACCCATG AACTTGGAAT TCTGCAGAGC CTTCAGCACC CACAACTGAT CAGCCTTCTT
    GACACTTTTG AGACACCAGC AAGCTATGTC TTAGTACTTG AGATAGCTGA TCAGGGATGT
    TTGTTAGAAT ATGTGGTACA GTGGGGAAAC CTTACAGAAG AAAAGATCAC CTCATATATG
    AGAGAGGTGC TGGAGGCCCT GCAGTATCTT CATAATTGCA GAATAGCACA TCTGGACCTA
    AAGCCTGAGA ACATTCTTGT GGATCAGAGC CTAACCAAAC CAACTATAAA ACTGTCAGAC
    TTTGGCGATG CTGTTCAGCT TAATACCACT TACTACATTC ACCAATTGTT GGGCAGTGCA
    GAGTTTGCCG CTCCAGAGAT CATCCTTGGG AATCCTGTCT CCCTTACCTC TGATATATGG
    AGTATTGGAG TTCTTACTTA TGTCCTTCTC AGTGGCGTCT CTCCTTTCCT TGATGAAAGT
    GTTGAAGAGA CATGCCTGAA CATTTGCCGA CTAGACTTTA GTTTTCCCGA TGACTACTTT
    AAGGGAGTAA GTCAGAAGGC CAAAGAATTT GTATGTTTCT TACTGCAGGG TGACCAGACA
    AAGCGCCCTT CTGCTGCTGT ATGTCTAAAG GAACAGTGGT TGCAGCCAGG CCAGCACAAA
    CGTAGTGGCA CATTAGACAT GTCAAGACTG ACCTCGTTTA TTGAACGCCG TAAACACCAG
    AATGATGTTC GACCCATTGG TAGCATTAAA AACTTCCTTC AGAGTCGCCT TTTACCCAGA
    ATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32254
    Clone ID Related Accession (Same CDS sequence) XM_012964354.2 , XM_012964354.3
    Accession Version XM_012964354.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9246bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product triple functional domain protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012964354.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGAGCACAT CCAGCACCGC AAGCTCCGGT AGCGCAAATA CCGGTACCGG CAATGCCGTT 
    GGAAGCAGCG GAGGCAACAC CGGGGAGGGG GCGGAGGAGA CGGCCAAGGA CTCTGCAGAC
    ATAGCTGCCT TTTACAGATC CGGTTTTCGT AGAAATGATG AATTGAAAGC AATGGAGGTG
    TTGCCAATTT TAAAAGAAAA AGTGGCATAT CTATCAGGTG GCAGAGATAA ACGAGGTGGT
    CCTGTATTAA CATTTCCAGC ACGCAGCAAC CATGATCGAA TACGACAAGA GGATCTCCGA
    AGACTGATTT CATATCTTGC TTGTATTCCT AGTGAGGAGG TGTGCAAGCG AGGCTTCACA
    GTGATTGTAG ATATGCGTGG TTCCAAGTGG GATTCTATTA AACCCTTGCT GAAAATTTTA
    CAAGAATCCT TTCCATGCTG TATTCATGTT GCACTAATTA TCAAACCAGA TAACTTCTGG
    CAGAAGCAAA GAACTAATTT TGGAAGTTCT AAGTTTGAAT TTGAGACAAG TATGGTGTCT
    TTAGAAGGCC TTACCAAAGT AGTTGACCCT TCCCAGCTAA CTCCAGAATT TGAGGGTTGC
    TTGGAATACA ACCATGAAGA GTGGATAGAG ATCAGAGTAG CATTTGAGGA TTTTATTAAT
    AATGCAACTC ATATGCTGTC TCGATTAGAG GAATTACAGG AGATGCTTGC AATTAAAGAG
    TTGCCTCAGG ATTTAGAGGG GGCACGCAAT ATGATTGAAG AACATTCTCA AATGAAAAAA
    AGGGTTATAA AGGCTCCTAT TGAAGATCTT GATTTAGAAG GGCAAAAGCT TTTACAAACA
    ATACAAAGCA GTGATAGCTT TCCTAAGAAG AACTCTAGTT CTGGAAATGC AGACCTTCAA
    AATCTTCTTC CCAAAGTTTC CACAATGTTA GACAGGCTTC ATTCAACACG ACAACATCTT
    CATCAGATGT GGCACATGCG AAAAGTAAAA CTGGACCAGT GCTTTCAGCT AAGGTTGTTT
    GAGCAGGATG CTGAGAAGAT GTTTGACTGG ATTACACACA ACAAAGGTCT GTTTCTGACA
    AGTTATACTG ACATTGGCAC CCGTAATAAT CATGCTATGG AACTTCAGAC ACAGCACAAT
    CATTTTGCTA TGAACTGCAT GAATGTCTAT GTAAACATTA GCCGAATAAT GTCTGTGGCA
    AATCGACTAG TAGAATCTGG ACACTATGCC TCACAGCAAA TTAAGCAGAT AGCTAACCAG
    TTGGAGCAAG AGTGGAAGGC TTTTGCCGCA GCCCTTGATG AACGTAGTAC GTTGCTTGAC
    ATGTCTGCAA TGTTCCATCA AAAAGCGGAG CAGTACATGG GCAATGTGGA TTCATGGTGC
    AAAGCTTGTG GGGAAGTGGA TCTTCCATCA GAGCTGCAGG ATCTAGAAGA TGCCATTCAT
    CACCATCAGG GAATATATGA GCATATCACT TTAGCATATT CAGAGGTTAG CCAGGATGGT
    AAATCTTTAT TAGACAAACT CCAGAGACCA TTGACTCCTG GTAGTTCTGA CTCACTGACA
    GCTTCTGCCA ACTATTCAAA GGCTGTGCAT CACGTCTTAG ATGCAATACA TGAAGTCTTG
    CACCACCAGC GTCAGTTGGA AAACATCTGG CAACATCGCA AAGTTCGTCT GCATCAACGC
    CTACAGCTCT GTGTTTTTCA GCAAGATGTT CAGCAGGTCT TGGATTGGAT AGAGAACCAT
    GGTGAAGCCT TTCTCAGCAA ACACACTGGG GTAGGCAAGT CTCTGCACAG GGCCAGAGCC
    TTGCAAAAGC GACATGAAGA CTTTGAAGAA GTCGCTCAGA ATACATACAC CAATGCTGAT
    AAGCTGCTGG AAGCTGCAGA ACAGTTGGCC CAGACAGGAG AGTGCGATCC AGAGGAAATA
    TACCAGGCTG CTCATCAGCT TGAGGATCGG ATTCAGGATT TTGTAAGACG TGTAGAACAA
    AGAAAAGTTC TCTTGGACAT GTCTGTATCA TTCCACTCGC ATGTTAAAGA GCTATGGACA
    TGGCTGGAGG ACCTACAAAA GGAACTGCTG GATGATGTCT ATGCGGAGTC AGTAGAAGCT
    GTACAGGACT TGATCAAACG GTTTGGACAA CAGCAGCAGA CAACCCTCCA GTTTACAGTA
    AATGTCATCA AAGAAGGGGA GGATCTAATT CAGCAACTAA GAGATTCTGC TATATCCAGC
    AACAAAACTC CTCACAACAG CTCAATCAAT CACATTGAGA CTGTCCTTCA ACAACTTGAT
    GAGGCCCAAT CACAGATGGA GGAGCTTTTC CAAGAGAGAA AAATCAAACT TGAGCTATTC
    TTGCAGCTTC GCATCTTTGA GAGAGATGCC ATTGATATCA TCTCAGACTT GGAATCATGG
    AATGATGAAC TTTCTCAGCA AATGAATGAT TTTGACACAG AGGACCTCAC ACTAGCCGAG
    CAAAGGCTAC AGCATCACGC AGACAAAGCT CTGACAATGA ACAACCTGAC ATTTGATGTC
    ATCCACCAAG GACAAGAGTT ACTACAATAT GTGAATGAAG TTCAGAACTC AGGGGTTGAG
    CTACTCTGTG ATAGAGATGT AGATATGGCT ACTAGAGTTC AGGACCTGTT GGAATTCCTC
    CATGAGAAAC AACAGGAGTT GGATCTAGCA GCTGAGCAGC ACCGAAAACA TCTAGAACAA
    TGTGTGCAAC TACGACATTT GCAAGCTGAA GTCAAACAGG TACTCGGCTG GATACGGAAT
    GGAGAGTCTA TGTTAAATGC CGGGCTTATC ACTGCCAGTT CATTGCAAGA AGCAGAGCAA
    CTTCAAAGGG AACATGAGCA GTTCCAGCAT GCAATAGAAA AAACCCACCA GAGTGCTCTG
    CAGGTGCAGC AGAAGGCAGA GGCCATGCTT CAAGCAAACC ATTACGACAT GGATATGATC
    CGGGACTGTG CTGAAAAAGT GGCCTCTCAT TGGCAACAGT TGATGCTTAA AATGGAAGAC
    CGTCTTAAGC TTGTCAATGC CTCTGTGGCC TTTTATAAAA CCTCAGAGCA GGTATGCAGT
    GTTCTAGAGA GCCTAGAGCA GGAGTACAAG CGGGAGGAAG ATTGGTGTGG CGGAGCTGAT
    AAACTTGGGC CTAACTCAGA GACTGACCAT GTTACTCCTA TGATCAGCAA ACACCTTGAA
    CAAAAGGAAG CCTTTCTTAA GGCCTGCACT TTGGCTCGCA GAAATGCTGA TGTCTTTCTG
    AAATACTTGC ACAGGAATAG TGTCAATATG CCTGGAATGG TGACACATGT AAAGGCTCCT
    GAACAGCAAG TAAAAAATAT TTTAAATGAG CTGTTTCAGA GGGAAAATAG AGTTCTTCAT
    TATTGGACCT TGCGGAAAAG GAGACTAGAC CATTGCCAGC AGTATGTAGT ATTTGAAAGA
    AGTGCCAAAC AGGCTCTGGA GTGGATCCAA GATAATGGAG AATTTTACAT TTCCACACAC
    ACCTCCACTG GCTCCACAAT TCAGCATACA CAAGAACTCT TAAAAGAGCA TGAGGAGTTT
    CAGATCACAG CAAAGCAAAC CAAAGAGAGG GTAAAGCTGT TGATACAGCT GGCTGATGGC
    TTTTGTGAAA AAGGGCATGC TCATGCTGCA GAAATTAAAA AATGTGTTAC AGCGGTGGAC
    AAGAGGTACA GAGACTTCTC GCTGCGTATG GAAAAGTACA GAACCAGCTT AGAGAAGGCT
    TTGGGCATTT CTTCTGATTC CAAGTCGAGT AAAAGCCTTC AGTTGGATAT TATTCCAGCC
    ACTGCCCCTG GTTCCGAAGT CAAGCTTCGT GATGCAACAC ATGAACTCAA TGAGGAGAAA
    AGAAAATCTG CTCGTCGAAA AGAATTCATC ATGGCAGAAC TTATACAAAC TGAAAAAGCA
    TATGTTCGTG ATCTTCGGGA ATGCATGGAT ACATATCTGT GGGAAATGAC AAGCGGAGTT
    GAAGAGATCC CACCAGGCAT TGTAAATAAA GAACACATTA TCTTTGGAAA CATGCAGGAA
    ATATATGAAT TTCATAACAA CATATTCTTA AAGGAGCTTG AGAAGTATGA GCAGCTACCT
    GAAGATGTTG GACATTGTTT TGTCACATGG GCTGACAAAT TTCAGATGTA TGTGTCCTAC
    TGTAAGAATA AGCCTGATTC AACACAACTT ATCTTGGAAC AAGCAGGAAC ATATTTTGAT
    GAAATTCAGC AGCGCCATGA GCTGGTCAAC TCCATCTCTT CTTATCTTAT CAAACCTGTG
    CAAAGGATAA CAAAATATCA GCTTCTTTTA AAAGAGCTGC TGACATGCTG CGAAGAAGGT
    AAAGGAGAGA TTAAAGATGG GCTGGAAGTG ATGCTAAGTG TACCAAAAAG AGCCAACGAT
    GCAATGCATC TCAGCATGCT TGAAGGGTTT GATGAGAACA TTGAATCTCA GGGAGAACTT
    ATTCTGCAAG AGTCTTTTCA AGTGTGGGAT CCTAAAACCT TAATAAGAAA GGGGCGTGAG
    AGACATCTCT TCTTGTTTGA AATGTCTTTG GTCTTCAGCA AAGAAGTGAA AGATTCAAGT
    GGAAGGAGCA AATATATTTA CAAATCAAAA TTATTTACCT CAGAGCTTGG AGTCACAGAA
    CATGTAGAAG GTGATCCTTG CAAATTTGCA CTTTGGGTAG GCAGAACGCC AACATCTGAT
    AACAAAATAG TCCTTAAGGG CTCAAGTATT GAGAACAAGC AAGATTGGAT CAAGCATATA
    AGAGAAGTGA TCCAAGACCG AACCATTCAC CTGAGAGGCG CATTAAAAGA GCCTATTCAT
    ATACCTAAAA CAGCTGCTGC TACAAAACAG AAACCTGCCA AAAGAGATGG AGAAGACCTG
    GACAGTCAAG GAGATGGCAG CAGCCAACCT GATACAATAT CTATTGCTTC ACGAACTTCA
    CAGAACACAT TAGACAGTGA TAAGCTTTCT GGTGGATGTG AGCTGACAGT AGTCATCCAT
    GACTTTACTG CCTGTAATAG CAATGAGCTG ACAATTAAAC GAGGGCAGAC TGTGGAGGTT
    CTAGAGCGGC ATCACGATCG ACCTGACTGG TGTTTAGTTC GGACTACTGA CCGGTCACCT
    GCAGCAGAGG GCTTGGTGCC TAGTAGTTCA CTGTGTATTG CACATTCTAG AAGTAGCATG
    GAAATGGAAG GCATATTTAA CCACAAAGAT TCCCTCTCTG TCTCCAGTAA TGATGCCAGT
    CCTCCTGCTT CAGTAATTTC CCTTCAGCCT CACATGATTG GTGCTCAAAG CTCTCCTGGC
    CCAAAGCGAC CTGGCAATAC TTTGAGAAAA TGGCTCACCA GCCCTGTCAG GAGACTTAGC
    AGTGGCAAAG CTGATGGACA TGTGAAAAAA TTGGCTCATA AACACAAAAA AAGCAGGGAG
    GTTCGTAAAA ACACAGACTC AGGTTCCCAA AAGGACTCTG ATGACAGTGC AGCAACACCT
    CAAGATGAAA CTATCGAGGA GAGAGTGCGC AATGAAGGAC TTAGCAGTGG TACGCTTTCT
    AAATCCTCCT CGTCTGGCAT GCAAAGCTGT GGAGAAGAAG AGGGCGAGGA AGGACCTGAT
    GCAGTTCCAT TGCCTCCACC CATGGCTATT CAGCAGCACA GCCTTCTTCA GCCTGACTCA
    CAGGATGACA AGACATCATC TCGGTTGCTG GTGCGACCTA CTAGCTCAGA GACACCTAGT
    GCTGCAGAGC TTGTTAGTGC TATTGAAGAA CTTGTAAAGA GTAAAATGGC TTTGGAAGAC
    CGGCCAAGTT CACTTCAAGT AGACCAGGGA GACAGTAGTA GCCCATCATT CAACCCTTCA
    GATACATCTC TGCTTTCCTC CTCTTCTCCA ATTGATGAAA TGGAAGAAAA AAAATCAAGC
    TCTTTAAAAA GAAGGCACTA TGTATTACAA GAGCTAGTAG AAACTGAACG CGATTATGTT
    CGGGACCTTG GCTATGTGGT TGAGGGTTAT ATGGCACTTA TGAAGGAGGA TGGAGTTCCA
    GATGATATGA AAGGAAAAGA CAAGATTGTT TTTGGCAACT TACACCAGAT CTACGAGTGG
    CACAGAGACT TTTTTTTGGG TGAACTGGAA AGGTGCCTGG ATGAGCCTGA AAGATTGGCA
    TCCCTATTTG TCAAGCATGA AAGAAGATTG CATATGTACA TAGTTTACTG CCAAAATAAA
    CCAAAGTCTG AACATATTGT TTCAGAATAC ATTGATACCT ATTTTGAGGA CCTGAAACAG
    CGTCTGGGCC ATCGACTTCA GCTTACAGAT CTGTTAATAA AACCCATACA AAGAATAATG
    AAATATCAGC TGCTATTAAA GGATTTTCTC AAGTACTCTA AGAAGGCTTG CATAGATGTA
    GCAGAACTTG AGAGAGCTGT GGAAGTCATG TGTATCGTAC CGAAGCGGTG TAATGACATG
    ATGAATGTTG GTCGTTTGCA AGGATTTGAT GGCAAAATAG TTGCCCAAGG AAAGCTGCTA
    CTGCAAGACA CATTCCTTGT GACTGAGCAG GACGCTGGTC TTCTGCCCCG ATGCAAAGAA
    AGGCGGGTCT TCCTTTTCGA ACAAATTGTC ATCTTTAGTG AGCCCTTAGA TAAAAAGAAA
    GGATACTCTA CACCTGGATT TCTTTTTAAA AATAATATCA AAGTGAGCTG CCTTAGTTTA
    GAGGAGTGTG TTGATAGTGA TCCATGCAAG TTTGCACTAT TGTCAAGGTC TGCTGACTCT
    ACAGAGATGT TTGTTTTGCA TTCTTCATCG CCTGGAGTGA GACAGTTATG GATTCATGAA
    ATCAACCAAA TTTTGGAAAA CCAGCGCAAT TTTTTAAATG CCTTGACATC ACCAATTGAA
    TACCACAGAA ATCACACTGG TGGAAGCAGT AGTGGCAACG TCACCTTTAC CAGCAGTCCC
    AGCAATTGTA GTGGGCTGGG TGGCTCCAGC CGAAGCAGGC CCTCTCGGAT TCCCCAGCCT
    GTTAGACACC ACTCTCCAGT CCTGGTCTCC TCTGCAGCTT CGGCACAGTC AGAGGCAGAC
    AAGATGTCAG GTATGTCAGC TCGCCGTGCA CCAGTTGTAC CTCTCAACCT CTCCTTAGAC
    AATAACCCCA GCCAGAAAGG TAGAGTAACT GCTGCTCCAG AGACAGGCGG GCGAGATAAA
    GAATCCAATT CAGTGCCAAA GATGAAAATG CTTGATGGCC CCAGGAAATC AGCTAGTGGC
    ATTCCAACCT TAGCTGATGG TGCACAGAAA GAATTGCGTG GGAGCACAGA AGACAGTCGA
    ATTAGAAGCA CTATGGGCAC TCTAACCTTA GGAAAGCCAA GAAATGGTTC TGCCTCACCC
    CTCAACTCTC CTGTCACAAT TAATTTTCCA TCGTCTTTTG GTAAGGAATC AGGGCCACCA
    AGCAGTCCCC TTCAGAAAGG CTCTTTTTGG AGCTCAATTC CAGGATCTCC AGCCAGCCGA
    CCTGGCTCCT TCACTTTCCC TGGAGACAGT GACTCTCTCC AGCGACAAGT TCATCGGCAC
    TCCACACACA GCAAGGATAC AGACCGCATG AGCACCTGCT CCTCGACTAG TGAGCAGTCC
    GTTCACTCCA CTCAGAGTAA TGGGAGTGAA AGTAGCAGCA GTAGCAATAT CTCCACCATG
    CTGGTTACGC ACGATTATAC AGCGGTGAAG GAGGATGAGA TCAATGTCTA CCAGGGAGAG
    GTTGTACAAA TTCTGGCAAG TAACCAACAG AACATGTTTC TGGTGTTCAG AGCTGCCACT
    GATGAGTGCC CTGCAGCTGA GGGCTGGATT CCAAGCTATG TTCTGGGTCA CACAAGTGCA
    ATCATCACTG ATATGACTGA TGCAACTATG AAGAAGTCCT CTTCTTGGCA TACAGCCCTT
    AGAATAAGAA AGAAATCTGA GAAGAGGGAA AAAGATGGCA GCAGAGACAC AAAACTAGAG
    AATGGTTATC GCAAATCAAG AGAAGGACTA GCTAATAAGG TATCAGTTAA GCTCCTGAAT
    CCAAACTACA TTTATGATGC TCCTCCAGAG TTTATTGTAC CACTGAACGA GGTTACATGT
    GACACAGGAG AGACTGTGAT TATTCGATGC AAAGTTTGTG GTCGCCCCAA GGCATCCATT
    ACATGGAAAG GTCCCGACCA TAATACTTTG AGCAACGATG GACACTACAG TATATCCTAC
    AGTGATCTTG GAGAAGCAAC TCTAAAAATA GTTGGTGTGA CCACTGAGGA TGATGGTATA
    TACACCTGCA TTGCAACAAA TGACTTGGGT TCAGTATCCG CTTCCTCCAA TCTTAGAGTG
    TTAGGTTCAT GGAGTGATGG ACTGAGAGTA ATCTGGAAAG AAAACTTCGA CATTGCTTAT
    AGTGAAGTGG CTGAACTTGG CAGGGGCAGG TTTTCTGTCG TGAAAAAATG TGAACAAAGA
    GTTACAAGAC GCATAGTAGC CACCAAATTT GTTAACAAGA AGTTGATGAA AAGAGACCAG
    GTTACCCATG AACTTGGAAT TCTGCAGAGC CTTCAGCACC CACAACTGAT CAGCCTTCTT
    GACACTTTTG AGACACCAGC AAGCTATGTC TTAGTACTTG AGATAGCTGA TCAGGGATGT
    TTGTTAGAAT ATGTGGTACA GTGGGGAAAC CTTACAGAAG AAAAGATCAC CTCATATATG
    AGAGAGGTGC TGGAGGCCCT GCAGTATCTT CATAATTGCA GAATAGCACA TCTGGACCTA
    AAGCCTGAGA ACATTCTTGT GGATCAGAGC CTAACCAAAC CAACTATAAA ACTGTCAGAC
    TTTGGCGATG CTGTTCAGCT TAATACCACT TACTACATTC ACCAATTGTT GGGCAGTGCA
    GAGTTTGCCG CTCCAGAGAT CATCCTTGGG AATCCTGTCT CCCTTACCTC TGATATATGG
    AGTATTGGAG TTCTTACTTA TGTCCTTCTC AGTGGCGTCT CTCCTTTCCT TGATGAAAGT
    GTTGAAGAGA CATGCCTGAA CATTTGCCGA CTAGACTTTA GTTTTCCCGA TGACTACTTT
    AAGGGAGTAA GTCAGAAGGC CAAAGAATTT GTATGTTTCT TACTGCAGGG TGACCAGACA
    AAGCGCCCTT CTGCTGCTGT ATGTCTAAAG GAACAGTGGT TGCAGCCAGG CCAGCACAAA
    CGTAGTGGCA CATTAGACAT GTCAAGACTG ACCTCGTTTA TTGAACGCCG TAAACACCAG
    AATGATGTTC GACCCATTGG TAGCATTAAA AACTTCCTTC AGAGTCGCCT TTTACCCAGA
    ATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012819808.1
    CDS527..9772
    Translation

    Target ORF information:

    RefSeq Version XM_012964354.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012964354.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGAGCACAT CCAGCACCGC AAGCTCCGGT AGCGCAAATA CCGGTACCGG CAATGCCGTT 
    GGAAGCAGCG GAGGCAACAC CGGGGAGGGG GCGGAGGAGA CGGCCAAGGA CTCTGCAGAC
    ATAGCTGCCT TTTACAGATC CGGTTTTCGT AGAAATGATG AATTGAAAGC AATGGAGGTG
    TTGCCAATTT TAAAAGAAAA AGTGGCATAT CTATCAGGTG GCAGAGATAA ACGAGGTGGT
    CCTGTATTAA CATTTCCAGC ACGCAGCAAC CATGATCGAA TACGACAAGA GGATCTCCGA
    AGACTGATTT CATATCTTGC TTGTATTCCT AGTGAGGAGG TGTGCAAGCG AGGCTTCACA
    GTGATTGTAG ATATGCGTGG TTCCAAGTGG GATTCTATTA AACCCTTGCT GAAAATTTTA
    CAAGAATCCT TTCCATGCTG TATTCATGTT GCACTAATTA TCAAACCAGA TAACTTCTGG
    CAGAAGCAAA GAACTAATTT TGGAAGTTCT AAGTTTGAAT TTGAGACAAG TATGGTGTCT
    TTAGAAGGCC TTACCAAAGT AGTTGACCCT TCCCAGCTAA CTCCAGAATT TGAGGGTTGC
    TTGGAATACA ACCATGAAGA GTGGATAGAG ATCAGAGTAG CATTTGAGGA TTTTATTAAT
    AATGCAACTC ATATGCTGTC TCGATTAGAG GAATTACAGG AGATGCTTGC AATTAAAGAG
    TTGCCTCAGG ATTTAGAGGG GGCACGCAAT ATGATTGAAG AACATTCTCA AATGAAAAAA
    AGGGTTATAA AGGCTCCTAT TGAAGATCTT GATTTAGAAG GGCAAAAGCT TTTACAAACA
    ATACAAAGCA GTGATAGCTT TCCTAAGAAG AACTCTAGTT CTGGAAATGC AGACCTTCAA
    AATCTTCTTC CCAAAGTTTC CACAATGTTA GACAGGCTTC ATTCAACACG ACAACATCTT
    CATCAGATGT GGCACATGCG AAAAGTAAAA CTGGACCAGT GCTTTCAGCT AAGGTTGTTT
    GAGCAGGATG CTGAGAAGAT GTTTGACTGG ATTACACACA ACAAAGGTCT GTTTCTGACA
    AGTTATACTG ACATTGGCAC CCGTAATAAT CATGCTATGG AACTTCAGAC ACAGCACAAT
    CATTTTGCTA TGAACTGCAT GAATGTCTAT GTAAACATTA GCCGAATAAT GTCTGTGGCA
    AATCGACTAG TAGAATCTGG ACACTATGCC TCACAGCAAA TTAAGCAGAT AGCTAACCAG
    TTGGAGCAAG AGTGGAAGGC TTTTGCCGCA GCCCTTGATG AACGTAGTAC GTTGCTTGAC
    ATGTCTGCAA TGTTCCATCA AAAAGCGGAG CAGTACATGG GCAATGTGGA TTCATGGTGC
    AAAGCTTGTG GGGAAGTGGA TCTTCCATCA GAGCTGCAGG ATCTAGAAGA TGCCATTCAT
    CACCATCAGG GAATATATGA GCATATCACT TTAGCATATT CAGAGGTTAG CCAGGATGGT
    AAATCTTTAT TAGACAAACT CCAGAGACCA TTGACTCCTG GTAGTTCTGA CTCACTGACA
    GCTTCTGCCA ACTATTCAAA GGCTGTGCAT CACGTCTTAG ATGCAATACA TGAAGTCTTG
    CACCACCAGC GTCAGTTGGA AAACATCTGG CAACATCGCA AAGTTCGTCT GCATCAACGC
    CTACAGCTCT GTGTTTTTCA GCAAGATGTT CAGCAGGTCT TGGATTGGAT AGAGAACCAT
    GGTGAAGCCT TTCTCAGCAA ACACACTGGG GTAGGCAAGT CTCTGCACAG GGCCAGAGCC
    TTGCAAAAGC GACATGAAGA CTTTGAAGAA GTCGCTCAGA ATACATACAC CAATGCTGAT
    AAGCTGCTGG AAGCTGCAGA ACAGTTGGCC CAGACAGGAG AGTGCGATCC AGAGGAAATA
    TACCAGGCTG CTCATCAGCT TGAGGATCGG ATTCAGGATT TTGTAAGACG TGTAGAACAA
    AGAAAAGTTC TCTTGGACAT GTCTGTATCA TTCCACTCGC ATGTTAAAGA GCTATGGACA
    TGGCTGGAGG ACCTACAAAA GGAACTGCTG GATGATGTCT ATGCGGAGTC AGTAGAAGCT
    GTACAGGACT TGATCAAACG GTTTGGACAA CAGCAGCAGA CAACCCTCCA GTTTACAGTA
    AATGTCATCA AAGAAGGGGA GGATCTAATT CAGCAACTAA GAGATTCTGC TATATCCAGC
    AACAAAACTC CTCACAACAG CTCAATCAAT CACATTGAGA CTGTCCTTCA ACAACTTGAT
    GAGGCCCAAT CACAGATGGA GGAGCTTTTC CAAGAGAGAA AAATCAAACT TGAGCTATTC
    TTGCAGCTTC GCATCTTTGA GAGAGATGCC ATTGATATCA TCTCAGACTT GGAATCATGG
    AATGATGAAC TTTCTCAGCA AATGAATGAT TTTGACACAG AGGACCTCAC ACTAGCCGAG
    CAAAGGCTAC AGCATCACGC AGACAAAGCT CTGACAATGA ACAACCTGAC ATTTGATGTC
    ATCCACCAAG GACAAGAGTT ACTACAATAT GTGAATGAAG TTCAGAACTC AGGGGTTGAG
    CTACTCTGTG ATAGAGATGT AGATATGGCT ACTAGAGTTC AGGACCTGTT GGAATTCCTC
    CATGAGAAAC AACAGGAGTT GGATCTAGCA GCTGAGCAGC ACCGAAAACA TCTAGAACAA
    TGTGTGCAAC TACGACATTT GCAAGCTGAA GTCAAACAGG TACTCGGCTG GATACGGAAT
    GGAGAGTCTA TGTTAAATGC CGGGCTTATC ACTGCCAGTT CATTGCAAGA AGCAGAGCAA
    CTTCAAAGGG AACATGAGCA GTTCCAGCAT GCAATAGAAA AAACCCACCA GAGTGCTCTG
    CAGGTGCAGC AGAAGGCAGA GGCCATGCTT CAAGCAAACC ATTACGACAT GGATATGATC
    CGGGACTGTG CTGAAAAAGT GGCCTCTCAT TGGCAACAGT TGATGCTTAA AATGGAAGAC
    CGTCTTAAGC TTGTCAATGC CTCTGTGGCC TTTTATAAAA CCTCAGAGCA GGTATGCAGT
    GTTCTAGAGA GCCTAGAGCA GGAGTACAAG CGGGAGGAAG ATTGGTGTGG CGGAGCTGAT
    AAACTTGGGC CTAACTCAGA GACTGACCAT GTTACTCCTA TGATCAGCAA ACACCTTGAA
    CAAAAGGAAG CCTTTCTTAA GGCCTGCACT TTGGCTCGCA GAAATGCTGA TGTCTTTCTG
    AAATACTTGC ACAGGAATAG TGTCAATATG CCTGGAATGG TGACACATGT AAAGGCTCCT
    GAACAGCAAG TAAAAAATAT TTTAAATGAG CTGTTTCAGA GGGAAAATAG AGTTCTTCAT
    TATTGGACCT TGCGGAAAAG GAGACTAGAC CATTGCCAGC AGTATGTAGT ATTTGAAAGA
    AGTGCCAAAC AGGCTCTGGA GTGGATCCAA GATAATGGAG AATTTTACAT TTCCACACAC
    ACCTCCACTG GCTCCACAAT TCAGCATACA CAAGAACTCT TAAAAGAGCA TGAGGAGTTT
    CAGATCACAG CAAAGCAAAC CAAAGAGAGG GTAAAGCTGT TGATACAGCT GGCTGATGGC
    TTTTGTGAAA AAGGGCATGC TCATGCTGCA GAAATTAAAA AATGTGTTAC AGCGGTGGAC
    AAGAGGTACA GAGACTTCTC GCTGCGTATG GAAAAGTACA GAACCAGCTT AGAGAAGGCT
    TTGGGCATTT CTTCTGATTC CAAGTCGAGT AAAAGCCTTC AGTTGGATAT TATTCCAGCC
    ACTGCCCCTG GTTCCGAAGT CAAGCTTCGT GATGCAACAC ATGAACTCAA TGAGGAGAAA
    AGAAAATCTG CTCGTCGAAA AGAATTCATC ATGGCAGAAC TTATACAAAC TGAAAAAGCA
    TATGTTCGTG ATCTTCGGGA ATGCATGGAT ACATATCTGT GGGAAATGAC AAGCGGAGTT
    GAAGAGATCC CACCAGGCAT TGTAAATAAA GAACACATTA TCTTTGGAAA CATGCAGGAA
    ATATATGAAT TTCATAACAA CATATTCTTA AAGGAGCTTG AGAAGTATGA GCAGCTACCT
    GAAGATGTTG GACATTGTTT TGTCACATGG GCTGACAAAT TTCAGATGTA TGTGTCCTAC
    TGTAAGAATA AGCCTGATTC AACACAACTT ATCTTGGAAC AAGCAGGAAC ATATTTTGAT
    GAAATTCAGC AGCGCCATGA GCTGGTCAAC TCCATCTCTT CTTATCTTAT CAAACCTGTG
    CAAAGGATAA CAAAATATCA GCTTCTTTTA AAAGAGCTGC TGACATGCTG CGAAGAAGGT
    AAAGGAGAGA TTAAAGATGG GCTGGAAGTG ATGCTAAGTG TACCAAAAAG AGCCAACGAT
    GCAATGCATC TCAGCATGCT TGAAGGGTTT GATGAGAACA TTGAATCTCA GGGAGAACTT
    ATTCTGCAAG AGTCTTTTCA AGTGTGGGAT CCTAAAACCT TAATAAGAAA GGGGCGTGAG
    AGACATCTCT TCTTGTTTGA AATGTCTTTG GTCTTCAGCA AAGAAGTGAA AGATTCAAGT
    GGAAGGAGCA AATATATTTA CAAATCAAAA TTATTTACCT CAGAGCTTGG AGTCACAGAA
    CATGTAGAAG GTGATCCTTG CAAATTTGCA CTTTGGGTAG GCAGAACGCC AACATCTGAT
    AACAAAATAG TCCTTAAGGG CTCAAGTATT GAGAACAAGC AAGATTGGAT CAAGCATATA
    AGAGAAGTGA TCCAAGACCG AACCATTCAC CTGAGAGGCG CATTAAAAGA GCCTATTCAT
    ATACCTAAAA CAGCTGCTGC TACAAAACAG AAACCTGCCA AAAGAGATGG AGAAGACCTG
    GACAGTCAAG GAGATGGCAG CAGCCAACCT GATACAATAT CTATTGCTTC ACGAACTTCA
    CAGAACACAT TAGACAGTGA TAAGCTTTCT GGTGGATGTG AGCTGACAGT AGTCATCCAT
    GACTTTACTG CCTGTAATAG CAATGAGCTG ACAATTAAAC GAGGGCAGAC TGTGGAGGTT
    CTAGAGCGGC ATCACGATCG ACCTGACTGG TGTTTAGTTC GGACTACTGA CCGGTCACCT
    GCAGCAGAGG GCTTGGTGCC TAGTAGTTCA CTGTGTATTG CACATTCTAG AAGTAGCATG
    GAAATGGAAG GCATATTTAA CCACAAAGAT TCCCTCTCTG TCTCCAGTAA TGATGCCAGT
    CCTCCTGCTT CAGTAATTTC CCTTCAGCCT CACATGATTG GTGCTCAAAG CTCTCCTGGC
    CCAAAGCGAC CTGGCAATAC TTTGAGAAAA TGGCTCACCA GCCCTGTCAG GAGACTTAGC
    AGTGGCAAAG CTGATGGACA TGTGAAAAAA TTGGCTCATA AACACAAAAA AAGCAGGGAG
    GTTCGTAAAA ACACAGACTC AGGTTCCCAA AAGGACTCTG ATGACAGTGC AGCAACACCT
    CAAGATGAAA CTATCGAGGA GAGAGTGCGC AATGAAGGAC TTAGCAGTGG TACGCTTTCT
    AAATCCTCCT CGTCTGGCAT GCAAAGCTGT GGAGAAGAAG AGGGCGAGGA AGGACCTGAT
    GCAGTTCCAT TGCCTCCACC CATGGCTATT CAGCAGCACA GCCTTCTTCA GCCTGACTCA
    CAGGATGACA AGACATCATC TCGGTTGCTG GTGCGACCTA CTAGCTCAGA GACACCTAGT
    GCTGCAGAGC TTGTTAGTGC TATTGAAGAA CTTGTAAAGA GTAAAATGGC TTTGGAAGAC
    CGGCCAAGTT CACTTCAAGT AGACCAGGGA GACAGTAGTA GCCCATCATT CAACCCTTCA
    GATACATCTC TGCTTTCCTC CTCTTCTCCA ATTGATGAAA TGGAAGAAAA AAAATCAAGC
    TCTTTAAAAA GAAGGCACTA TGTATTACAA GAGCTAGTAG AAACTGAACG CGATTATGTT
    CGGGACCTTG GCTATGTGGT TGAGGGTTAT ATGGCACTTA TGAAGGAGGA TGGAGTTCCA
    GATGATATGA AAGGAAAAGA CAAGATTGTT TTTGGCAACT TACACCAGAT CTACGAGTGG
    CACAGAGACT TTTTTTTGGG TGAACTGGAA AGGTGCCTGG ATGAGCCTGA AAGATTGGCA
    TCCCTATTTG TCAAGCATGA AAGAAGATTG CATATGTACA TAGTTTACTG CCAAAATAAA
    CCAAAGTCTG AACATATTGT TTCAGAATAC ATTGATACCT ATTTTGAGGA CCTGAAACAG
    CGTCTGGGCC ATCGACTTCA GCTTACAGAT CTGTTAATAA AACCCATACA AAGAATAATG
    AAATATCAGC TGCTATTAAA GGATTTTCTC AAGTACTCTA AGAAGGCTTG CATAGATGTA
    GCAGAACTTG AGAGAGCTGT GGAAGTCATG TGTATCGTAC CGAAGCGGTG TAATGACATG
    ATGAATGTTG GTCGTTTGCA AGGATTTGAT GGCAAAATAG TTGCCCAAGG AAAGCTGCTA
    CTGCAAGACA CATTCCTTGT GACTGAGCAG GACGCTGGTC TTCTGCCCCG ATGCAAAGAA
    AGGCGGGTCT TCCTTTTCGA ACAAATTGTC ATCTTTAGTG AGCCCTTAGA TAAAAAGAAA
    GGATACTCTA CACCTGGATT TCTTTTTAAA AATAATATCA AAGTGAGCTG CCTTAGTTTA
    GAGGAGTGTG TTGATAGTGA TCCATGCAAG TTTGCACTAT TGTCAAGGTC TGCTGACTCT
    ACAGAGATGT TTGTTTTGCA TTCTTCATCG CCTGGAGTGA GACAGTTATG GATTCATGAA
    ATCAACCAAA TTTTGGAAAA CCAGCGCAAT TTTTTAAATG CCTTGACATC ACCAATTGAA
    TACCACAGAA ATCACACTGG TGGAAGCAGT AGTGGCAACG TCACCTTTAC CAGCAGTCCC
    AGCAATTGTA GTGGGCTGGG TGGCTCCAGC CGAAGCAGGC CCTCTCGGAT TCCCCAGCCT
    GTTAGACACC ACTCTCCAGT CCTGGTCTCC TCTGCAGCTT CGGCACAGTC AGAGGCAGAC
    AAGATGTCAG GTATGTCAGC TCGCCGTGCA CCAGTTGTAC CTCTCAACCT CTCCTTAGAC
    AATAACCCCA GCCAGAAAGG TAGAGTAACT GCTGCTCCAG AGACAGGCGG GCGAGATAAA
    GAATCCAATT CAGTGCCAAA GATGAAAATG CTTGATGGCC CCAGGAAATC AGCTAGTGGC
    ATTCCAACCT TAGCTGATGG TGCACAGAAA GAATTGCGTG GGAGCACAGA AGACAGTCGA
    ATTAGAAGCA CTATGGGCAC TCTAACCTTA GGAAAGCCAA GAAATGGTTC TGCCTCACCC
    CTCAACTCTC CTGTCACAAT TAATTTTCCA TCGTCTTTTG GTAAGGAATC AGGGCCACCA
    AGCAGTCCCC TTCAGAAAGG CTCTTTTTGG AGCTCAATTC CAGGATCTCC AGCCAGCCGA
    CCTGGCTCCT TCACTTTCCC TGGAGACAGT GACTCTCTCC AGCGACAAGT TCATCGGCAC
    TCCACACACA GCAAGGATAC AGACCGCATG AGCACCTGCT CCTCGACTAG TGAGCAGTCC
    GTTCACTCCA CTCAGAGTAA TGGGAGTGAA AGTAGCAGCA GTAGCAATAT CTCCACCATG
    CTGGTTACGC ACGATTATAC AGCGGTGAAG GAGGATGAGA TCAATGTCTA CCAGGGAGAG
    GTTGTACAAA TTCTGGCAAG TAACCAACAG AACATGTTTC TGGTGTTCAG AGCTGCCACT
    GATGAGTGCC CTGCAGCTGA GGGCTGGATT CCAAGCTATG TTCTGGGTCA CACAAGTGCA
    ATCATCACTG ATATGACTGA TGCAACTATG AAGAAGTCCT CTTCTTGGCA TACAGCCCTT
    AGAATAAGAA AGAAATCTGA GAAGAGGGAA AAAGATGGCA GCAGAGACAC AAAACTAGAG
    AATGGTTATC GCAAATCAAG AGAAGGACTA GCTAATAAGG TATCAGTTAA GCTCCTGAAT
    CCAAACTACA TTTATGATGC TCCTCCAGAG TTTATTGTAC CACTGAACGA GGTTACATGT
    GACACAGGAG AGACTGTGAT TATTCGATGC AAAGTTTGTG GTCGCCCCAA GGCATCCATT
    ACATGGAAAG GTCCCGACCA TAATACTTTG AGCAACGATG GACACTACAG TATATCCTAC
    AGTGATCTTG GAGAAGCAAC TCTAAAAATA GTTGGTGTGA CCACTGAGGA TGATGGTATA
    TACACCTGCA TTGCAACAAA TGACTTGGGT TCAGTATCCG CTTCCTCCAA TCTTAGAGTG
    TTAGGTTCAT GGAGTGATGG ACTGAGAGTA ATCTGGAAAG AAAACTTCGA CATTGCTTAT
    AGTGAAGTGG CTGAACTTGG CAGGGGCAGG TTTTCTGTCG TGAAAAAATG TGAACAAAGA
    GTTACAAGAC GCATAGTAGC CACCAAATTT GTTAACAAGA AGTTGATGAA AAGAGACCAG
    GTTACCCATG AACTTGGAAT TCTGCAGAGC CTTCAGCACC CACAACTGAT CAGCCTTCTT
    GACACTTTTG AGACACCAGC AAGCTATGTC TTAGTACTTG AGATAGCTGA TCAGGGATGT
    TTGTTAGAAT ATGTGGTACA GTGGGGAAAC CTTACAGAAG AAAAGATCAC CTCATATATG
    AGAGAGGTGC TGGAGGCCCT GCAGTATCTT CATAATTGCA GAATAGCACA TCTGGACCTA
    AAGCCTGAGA ACATTCTTGT GGATCAGAGC CTAACCAAAC CAACTATAAA ACTGTCAGAC
    TTTGGCGATG CTGTTCAGCT TAATACCACT TACTACATTC ACCAATTGTT GGGCAGTGCA
    GAGTTTGCCG CTCCAGAGAT CATCCTTGGG AATCCTGTCT CCCTTACCTC TGATATATGG
    AGTATTGGAG TTCTTACTTA TGTCCTTCTC AGTGGCGTCT CTCCTTTCCT TGATGAAAGT
    GTTGAAGAGA CATGCCTGAA CATTTGCCGA CTAGACTTTA GTTTTCCCGA TGACTACTTT
    AAGGGAGTAA GTCAGAAGGC CAAAGAATTT GTATGTTTCT TACTGCAGGG TGACCAGACA
    AAGCGCCCTT CTGCTGCTGT ATGTCTAAAG GAACAGTGGT TGCAGCCAGG CCAGCACAAA
    CGTAGTGGCA CATTAGACAT GTCAAGACTG ACCTCGTTTA TTGAACGCCG TAAACACCAG
    AATGATGTTC GACCCATTGG TAGCATTAAA AACTTCCTTC AGAGTCGCCT TTTACCCAGA
    ATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32255
    Clone ID Related Accession (Same CDS sequence) XM_012964355.2 , XM_012964355.3
    Accession Version XM_012964355.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9246bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product triple functional domain protein isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964355.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    721
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    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
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    2641
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    3001
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    3181
    3241
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    4141
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    8401
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    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGAGCACAT CCAGCACCGC AAGCTCCGGT AGCGCAAATA CCGGTACCGG CAATGCCGTT 
    GGAAGCAGCG GAGGCAACAC CGGGGAGGGG GCGGAGGAGA CGGCCAAGGA CTCTGCAGAC
    ATAGCTGCCT TTTACAGATC CGGTTTTCGT AGAAATGATG AATTGAAAGC AATGGAGGTG
    TTGCCAATTT TAAAAGAAAA AGTGGCATAT CTATCAGGTG GCAGAGATAA ACGAGGTGGT
    CCTGTATTAA CATTTCCAGC ACGCAGCAAC CATGATCGAA TACGACAAGA GGATCTCCGA
    AGACTGATTT CATATCTTGC TTGTATTCCT AGTGAGGAGG TGTGCAAGCG AGGCTTCACA
    GTGATTGTAG ATATGCGTGG TTCCAAGTGG GATTCTATTA AACCCTTGCT GAAAATTTTA
    CAAGAATCCT TTCCATGCTG TATTCATGTT GCACTAATTA TCAAACCAGA TAACTTCTGG
    CAGAAGCAAA GAACTAATTT TGGAAGTTCT AAGTTTGAAT TTGAGACAAG TATGGTGTCT
    TTAGAAGGCC TTACCAAAGT AGTTGACCCT TCCCAGCTAA CTCCAGAATT TGAGGGTTGC
    TTGGAATACA ACCATGAAGA GTGGATAGAG ATCAGAGTAG CATTTGAGGA TTTTATTAAT
    AATGCAACTC ATATGCTGTC TCGATTAGAG GAATTACAGG AGATGCTTGC AATTAAAGAG
    TTGCCTCAGG ATTTAGAGGG GGCACGCAAT ATGATTGAAG AACATTCTCA AATGAAAAAA
    AGGGTTATAA AGGCTCCTAT TGAAGATCTT GATTTAGAAG GGCAAAAGCT TTTACAAACA
    ATACAAAGCA GTGATAGCTT TCCTAAGAAG AACTCTAGTT CTGGAAATGC AGACCTTCAA
    AATCTTCTTC CCAAAGTTTC CACAATGTTA GACAGGCTTC ATTCAACACG ACAACATCTT
    CATCAGATGT GGCACATGCG AAAAGTAAAA CTGGACCAGT GCTTTCAGCT AAGGTTGTTT
    GAGCAGGATG CTGAGAAGAT GTTTGACTGG ATTACACACA ACAAAGGTCT GTTTCTGACA
    AGTTATACTG ACATTGGCAC CCGTAATAAT CATGCTATGG AACTTCAGAC ACAGCACAAT
    CATTTTGCTA TGAACTGCAT GAATGTCTAT GTAAACATTA GCCGAATAAT GTCTGTGGCA
    AATCGACTAG TAGAATCTGG ACACTATGCC TCACAGCAAA TTAAGCAGAT AGCTAACCAG
    TTGGAGCAAG AGTGGAAGGC TTTTGCCGCA GCCCTTGATG AACGTAGTAC GTTGCTTGAC
    ATGTCTGCAA TGTTCCATCA AAAAGCGGAG CAGTACATGG GCAATGTGGA TTCATGGTGC
    AAAGCTTGTG GGGAAGTGGA TCTTCCATCA GAGCTGCAGG ATCTAGAAGA TGCCATTCAT
    CACCATCAGG GAATATATGA GCATATCACT TTAGCATATT CAGAGGTTAG CCAGGATGGT
    AAATCTTTAT TAGACAAACT CCAGAGACCA TTGACTCCTG GTAGTTCTGA CTCACTGACA
    GCTTCTGCCA ACTATTCAAA GGCTGTGCAT CACGTCTTAG ATGCAATACA TGAAGTCTTG
    CACCACCAGC GTCAGTTGGA AAACATCTGG CAACATCGCA AAGTTCGTCT GCATCAACGC
    CTACAGCTCT GTGTTTTTCA GCAAGATGTT CAGCAGGTCT TGGATTGGAT AGAGAACCAT
    GGTGAAGCCT TTCTCAGCAA ACACACTGGG GTAGGCAAGT CTCTGCACAG GGCCAGAGCC
    TTGCAAAAGC GACATGAAGA CTTTGAAGAA GTCGCTCAGA ATACATACAC CAATGCTGAT
    AAGCTGCTGG AAGCTGCAGA ACAGTTGGCC CAGACAGGAG AGTGCGATCC AGAGGAAATA
    TACCAGGCTG CTCATCAGCT TGAGGATCGG ATTCAGGATT TTGTAAGACG TGTAGAACAA
    AGAAAAGTTC TCTTGGACAT GTCTGTATCA TTCCACTCGC ATGTTAAAGA GCTATGGACA
    TGGCTGGAGG ACCTACAAAA GGAACTGCTG GATGATGTCT ATGCGGAGTC AGTAGAAGCT
    GTACAGGACT TGATCAAACG GTTTGGACAA CAGCAGCAGA CAACCCTCCA GTTTACAGTA
    AATGTCATCA AAGAAGGGGA GGATCTAATT CAGCAACTAA GAGATTCTGC TATATCCAGC
    AACAAAACTC CTCACAACAG CTCAATCAAT CACATTGAGA CTGTCCTTCA ACAACTTGAT
    GAGGCCCAAT CACAGATGGA GGAGCTTTTC CAAGAGAGAA AAATCAAACT TGAGCTATTC
    TTGCAGCTTC GCATCTTTGA GAGAGATGCC ATTGATATCA TCTCAGACTT GGAATCATGG
    AATGATGAAC TTTCTCAGCA AATGAATGAT TTTGACACAG AGGACCTCAC ACTAGCCGAG
    CAAAGGCTAC AGCATCACGC AGACAAAGCT CTGACAATGA ACAACCTGAC ATTTGATGTC
    ATCCACCAAG GACAAGAGTT ACTACAATAT GTGAATGAAG TTCAGAACTC AGGGGTTGAG
    CTACTCTGTG ATAGAGATGT AGATATGGCT ACTAGAGTTC AGGACCTGTT GGAATTCCTC
    CATGAGAAAC AACAGGAGTT GGATCTAGCA GCTGAGCAGC ACCGAAAACA TCTAGAACAA
    TGTGTGCAAC TACGACATTT GCAAGCTGAA GTCAAACAGG TACTCGGCTG GATACGGAAT
    GGAGAGTCTA TGTTAAATGC CGGGCTTATC ACTGCCAGTT CATTGCAAGA AGCAGAGCAA
    CTTCAAAGGG AACATGAGCA GTTCCAGCAT GCAATAGAAA AAACCCACCA GAGTGCTCTG
    CAGGTGCAGC AGAAGGCAGA GGCCATGCTT CAAGCAAACC ATTACGACAT GGATATGATC
    CGGGACTGTG CTGAAAAAGT GGCCTCTCAT TGGCAACAGT TGATGCTTAA AATGGAAGAC
    CGTCTTAAGC TTGTCAATGC CTCTGTGGCC TTTTATAAAA CCTCAGAGCA GGTATGCAGT
    GTTCTAGAGA GCCTAGAGCA GGAGTACAAG CGGGAGGAAG ATTGGTGTGG CGGAGCTGAT
    AAACTTGGGC CTAACTCAGA GACTGACCAT GTTACTCCTA TGATCAGCAA ACACCTTGAA
    CAAAAGGAAG CCTTTCTTAA GGCCTGCACT TTGGCTCGCA GAAATGCTGA TGTCTTTCTG
    AAATACTTGC ACAGGAATAG TGTCAATATG CCTGGAATGG TGACACATGT AAAGGCTCCT
    GAACAGCAAG TAAAAAATAT TTTAAATGAG CTGTTTCAGA GGGAAAATAG AGTTCTTCAT
    TATTGGACCT TGCGGAAAAG GAGACTAGAC CATTGCCAGC AGTATGTAGT ATTTGAAAGA
    AGTGCCAAAC AGGCTCTGGA GTGGATCCAA GATAATGGAG AATTTTACAT TTCCACACAC
    ACCTCCACTG GCTCCACAAT TCAGCATACA CAAGAACTCT TAAAAGAGCA TGAGGAGTTT
    CAGATCACAG CAAAGCAAAC CAAAGAGAGG GTAAAGCTGT TGATACAGCT GGCTGATGGC
    TTTTGTGAAA AAGGGCATGC TCATGCTGCA GAAATTAAAA AATGTGTTAC AGCGGTGGAC
    AAGAGGTACA GAGACTTCTC GCTGCGTATG GAAAAGTACA GAACCAGCTT AGAGAAGGCT
    TTGGGCATTT CTTCTGATTC CAAGTCGAGT AAAAGCCTTC AGTTGGATAT TATTCCAGCC
    ACTGCCCCTG GTTCCGAAGT CAAGCTTCGT GATGCAACAC ATGAACTCAA TGAGGAGAAA
    AGAAAATCTG CTCGTCGAAA AGAATTCATC ATGGCAGAAC TTATACAAAC TGAAAAAGCA
    TATGTTCGTG ATCTTCGGGA ATGCATGGAT ACATATCTGT GGGAAATGAC AAGCGGAGTT
    GAAGAGATCC CACCAGGCAT TGTAAATAAA GAACACATTA TCTTTGGAAA CATGCAGGAA
    ATATATGAAT TTCATAACAA CATATTCTTA AAGGAGCTTG AGAAGTATGA GCAGCTACCT
    GAAGATGTTG GACATTGTTT TGTCACATGG GCTGACAAAT TTCAGATGTA TGTGTCCTAC
    TGTAAGAATA AGCCTGATTC AACACAACTT ATCTTGGAAC AAGCAGGAAC ATATTTTGAT
    GAAATTCAGC AGCGCCATGA GCTGGTCAAC TCCATCTCTT CTTATCTTAT CAAACCTGTG
    CAAAGGATAA CAAAATATCA GCTTCTTTTA AAAGAGCTGC TGACATGCTG CGAAGAAGGT
    AAAGGAGAGA TTAAAGATGG GCTGGAAGTG ATGCTAAGTG TACCAAAAAG AGCCAACGAT
    GCAATGCATC TCAGCATGCT TGAAGGGTTT GATGAGAACA TTGAATCTCA GGGAGAACTT
    ATTCTGCAAG AGTCTTTTCA AGTGTGGGAT CCTAAAACCT TAATAAGAAA GGGGCGTGAG
    AGACATCTCT TCTTGTTTGA AATGTCTTTG GTCTTCAGCA AAGAAGTGAA AGATTCAAGT
    GGAAGGAGCA AATATATTTA CAAATCAAAA TTATTTACCT CAGAGCTTGG AGTCACAGAA
    CATGTAGAAG GTGATCCTTG CAAATTTGCA CTTTGGGTAG GCAGAACGCC AACATCTGAT
    AACAAAATAG TCCTTAAGGG CTCAAGTATT GAGAACAAGC AAGATTGGAT CAAGCATATA
    AGAGAAGTGA TCCAAGACCG AACCATTCAC CTGAGAGGCG CATTAAAAGA GCCTATTCAT
    ATACCTAAAA CAGCTGCTGC TACAAAACAG AAACCTGCCA AAAGAGATGG AGAAGACCTG
    GACAGTCAAG GAGATGGCAG CAGCCAACCT GATACAATAT CTATTGCTTC ACGAACTTCA
    CAGAACACAT TAGACAGTGA TAAGCTTTCT GGTGGATGTG AGCTGACAGT AGTCATCCAT
    GACTTTACTG CCTGTAATAG CAATGAGCTG ACAATTAAAC GAGGGCAGAC TGTGGAGGTT
    CTAGAGCGGC ATCACGATCG ACCTGACTGG TGTTTAGTTC GGACTACTGA CCGGTCACCT
    GCAGCAGAGG GCTTGGTGCC TAGTAGTTCA CTGTGTATTG CACATTCTAG AAGTAGCATG
    GAAATGGAAG GCATATTTAA CCACAAAGAT TCCCTCTCTG TCTCCAGTAA TGATGCCAGT
    CCTCCTGCTT CAGTAATTTC CCTTCAGCCT CACATGATTG GTGCTCAAAG CTCTCCTGGC
    CCAAAGCGAC CTGGCAATAC TTTGAGAAAA TGGCTCACCA GCCCTGTCAG GAGACTTAGC
    AGTGGCAAAG CTGATGGACA TGTGAAAAAA TTGGCTCATA AACACAAAAA AAGCAGGGAG
    GTTCGTAAAA ACACAGACTC AGGTTCCCAA AAGGACTCTG ATGACAGTGC AGCAACACCT
    CAAGATGAAA CTATCGAGGA GAGAGTGCGC AATGAAGGAC TTAGCAGTGG TACGCTTTCT
    AAATCCTCCT CGTCTGGCAT GCAAAGCTGT GGAGAAGAAG AGGGCGAGGA AGGACCTGAT
    GCAGTTCCAT TGCCTCCACC CATGGCTATT CAGCAGCACA GCCTTCTTCA GCCTGACTCA
    CAGGATGACA AGACATCATC TCGGTTGCTG GTGCGACCTA CTAGCTCAGA GACACCTAGT
    GCTGCAGAGC TTGTTAGTGC TATTGAAGAA CTTGTAAAGA GTAAAATGGC TTTGGAAGAC
    CGGCCAAGTT CACTTCAAGT AGACCAGGGA GACAGTAGTA GCCCATCATT CAACCCTTCA
    GATACATCTC TGCTTTCCTC CTCTTCTCCA ATTGATGAAA TGGAAGAAAA AAAATCAAGC
    TCTTTAAAAA GAAGGCACTA TGTATTACAA GAGCTAGTAG AAACTGAACG CGATTATGTT
    CGGGACCTTG GCTATGTGGT TGAGGGTTAT ATGGCACTTA TGAAGGAGGA TGGAGTTCCA
    GATGATATGA AAGGAAAAGA CAAGATTGTT TTTGGCAACT TACACCAGAT CTACGAGTGG
    CACAGAGACT TTTTTTTGGG TGAACTGGAA AGGTGCCTGG ATGAGCCTGA AAGATTGGCA
    TCCCTATTTG TCAAGCATGA AAGAAGATTG CATATGTACA TAGTTTACTG CCAAAATAAA
    CCAAAGTCTG AACATATTGT TTCAGAATAC ATTGATACCT ATTTTGAGGA CCTGAAACAG
    CGTCTGGGCC ATCGACTTCA GCTTACAGAT CTGTTAATAA AACCCATACA AAGAATAATG
    AAATATCAGC TGCTATTAAA GGATTTTCTC AAGTACTCTA AGAAGGCTTG CATAGATGTA
    GCAGAACTTG AGAGAGCTGT GGAAGTCATG TGTATCGTAC CGAAGCGGTG TAATGACATG
    ATGAATGTTG GTCGTTTGCA AGGATTTGAT GGCAAAATAG TTGCCCAAGG AAAGCTGCTA
    CTGCAAGACA CATTCCTTGT GACTGAGCAG GACGCTGGTC TTCTGCCCCG ATGCAAAGAA
    AGGCGGGTCT TCCTTTTCGA ACAAATTGTC ATCTTTAGTG AGCCCTTAGA TAAAAAGAAA
    GGATACTCTA CACCTGGATT TCTTTTTAAA AATAATATCA AAGTGAGCTG CCTTAGTTTA
    GAGGAGTGTG TTGATAGTGA TCCATGCAAG TTTGCACTAT TGTCAAGGTC TGCTGACTCT
    ACAGAGATGT TTGTTTTGCA TTCTTCATCG CCTGGAGTGA GACAGTTATG GATTCATGAA
    ATCAACCAAA TTTTGGAAAA CCAGCGCAAT TTTTTAAATG CCTTGACATC ACCAATTGAA
    TACCACAGAA ATCACACTGG TGGAAGCAGT AGTGGCAACG TCACCTTTAC CAGCAGTCCC
    AGCAATTGTA GTGGGCTGGG TGGCTCCAGC CGAAGCAGGC CCTCTCGGAT TCCCCAGCCT
    GTTAGACACC ACTCTCCAGT CCTGGTCTCC TCTGCAGCTT CGGCACAGTC AGAGGCAGAC
    AAGATGTCAG GTATGTCAGC TCGCCGTGCA CCAGTTGTAC CTCTCAACCT CTCCTTAGAC
    AATAACCCCA GCCAGAAAGG TAGAGTAACT GCTGCTCCAG AGACAGGCGG GCGAGATAAA
    GAATCCAATT CAGTGCCAAA GATGAAAATG CTTGATGGCC CCAGGAAATC AGCTAGTGGC
    ATTCCAACCT TAGCTGATGG TGCACAGAAA GAATTGCGTG GGAGCACAGA AGACAGTCGA
    ATTAGAAGCA CTATGGGCAC TCTAACCTTA GGAAAGCCAA GAAATGGTTC TGCCTCACCC
    CTCAACTCTC CTGTCACAAT TAATTTTCCA TCGTCTTTTG GTAAGGAATC AGGGCCACCA
    AGCAGTCCCC TTCAGAAAGG CTCTTTTTGG AGCTCAATTC CAGGATCTCC AGCCAGCCGA
    CCTGGCTCCT TCACTTTCCC TGGAGACAGT GACTCTCTCC AGCGACAAGT TCATCGGCAC
    TCCACACACA GCAAGGATAC AGACCGCATG AGCACCTGCT CCTCGACTAG TGAGCAGTCC
    GTTCACTCCA CTCAGAGTAA TGGGGTAAGG ACAGAGAGTG AAAGTAGCAG CAGTAGCAAT
    ATCTCCACCA TGCTGGTTAC GCACGATTAT ACAGCGGTGA AGGAGGATGA GATCAATGTC
    TACCAGGGAG AGGTTGTACA AATTCTGGCA AGTAACCAAC AGAACATGTT TCTGGTGTTC
    AGAGCTGCCA CTGATGAGTG CCCTGCAGCT GAGGGCTGGA TTCCAAGCTA TGTTCTGGGT
    CACACAAGTG CAATCATCAC TGATATGACT GATGCAACTA TGAAGAAGTC CTCTTCTTGG
    CATACAGCCC TTAGAATAAG AAAGAAATCT GAGAAGAGGG AAAAAGATGG CAGCAGAGAC
    ACAAAACTAG AGAATGGTTA TCGCAAATCA AGAGAAGGAC TAGCTAATAA GCTCCTGAAT
    CCAAACTACA TTTATGATGC TCCTCCAGAG TTTATTGTAC CACTGAACGA GGTTACATGT
    GACACAGGAG AGACTGTGAT TATTCGATGC AAAGTTTGTG GTCGCCCCAA GGCATCCATT
    ACATGGAAAG GTCCCGACCA TAATACTTTG AGCAACGATG GACACTACAG TATATCCTAC
    AGTGATCTTG GAGAAGCAAC TCTAAAAATA GTTGGTGTGA CCACTGAGGA TGATGGTATA
    TACACCTGCA TTGCAACAAA TGACTTGGGT TCAGTATCCG CTTCCTCCAA TCTTAGAGTG
    TTAGGTTCAT GGAGTGATGG ACTGAGAGTA ATCTGGAAAG AAAACTTCGA CATTGCTTAT
    AGTGAAGTGG CTGAACTTGG CAGGGGCAGG TTTTCTGTCG TGAAAAAATG TGAACAAAGA
    GTTACAAGAC GCATAGTAGC CACCAAATTT GTTAACAAGA AGTTGATGAA AAGAGACCAG
    GTTACCCATG AACTTGGAAT TCTGCAGAGC CTTCAGCACC CACAACTGAT CAGCCTTCTT
    GACACTTTTG AGACACCAGC AAGCTATGTC TTAGTACTTG AGATAGCTGA TCAGGGATGT
    TTGTTAGAAT ATGTGGTACA GTGGGGAAAC CTTACAGAAG AAAAGATCAC CTCATATATG
    AGAGAGGTGC TGGAGGCCCT GCAGTATCTT CATAATTGCA GAATAGCACA TCTGGACCTA
    AAGCCTGAGA ACATTCTTGT GGATCAGAGC CTAACCAAAC CAACTATAAA ACTGTCAGAC
    TTTGGCGATG CTGTTCAGCT TAATACCACT TACTACATTC ACCAATTGTT GGGCAGTGCA
    GAGTTTGCCG CTCCAGAGAT CATCCTTGGG AATCCTGTCT CCCTTACCTC TGATATATGG
    AGTATTGGAG TTCTTACTTA TGTCCTTCTC AGTGGCGTCT CTCCTTTCCT TGATGAAAGT
    GTTGAAGAGA CATGCCTGAA CATTTGCCGA CTAGACTTTA GTTTTCCCGA TGACTACTTT
    AAGGGAGTAA GTCAGAAGGC CAAAGAATTT GTATGTTTCT TACTGCAGGG TGACCAGACA
    AAGCGCCCTT CTGCTGCTGT ATGTCTAAAG GAACAGTGGT TGCAGCCAGG CCAGCACAAA
    CGTAGTGGCA CATTAGACAT GTCAAGACTG ACCTCGTTTA TTGAACGCCG TAAACACCAG
    AATGATGTTC GACCCATTGG TAGCATTAAA AACTTCCTTC AGAGTCGCCT TTTACCCAGA
    ATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012819809.1
    CDS616..9861
    Translation

    Target ORF information:

    RefSeq Version XM_012964355.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012964355.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGAGCACAT CCAGCACCGC AAGCTCCGGT AGCGCAAATA CCGGTACCGG CAATGCCGTT 
    GGAAGCAGCG GAGGCAACAC CGGGGAGGGG GCGGAGGAGA CGGCCAAGGA CTCTGCAGAC
    ATAGCTGCCT TTTACAGATC CGGTTTTCGT AGAAATGATG AATTGAAAGC AATGGAGGTG
    TTGCCAATTT TAAAAGAAAA AGTGGCATAT CTATCAGGTG GCAGAGATAA ACGAGGTGGT
    CCTGTATTAA CATTTCCAGC ACGCAGCAAC CATGATCGAA TACGACAAGA GGATCTCCGA
    AGACTGATTT CATATCTTGC TTGTATTCCT AGTGAGGAGG TGTGCAAGCG AGGCTTCACA
    GTGATTGTAG ATATGCGTGG TTCCAAGTGG GATTCTATTA AACCCTTGCT GAAAATTTTA
    CAAGAATCCT TTCCATGCTG TATTCATGTT GCACTAATTA TCAAACCAGA TAACTTCTGG
    CAGAAGCAAA GAACTAATTT TGGAAGTTCT AAGTTTGAAT TTGAGACAAG TATGGTGTCT
    TTAGAAGGCC TTACCAAAGT AGTTGACCCT TCCCAGCTAA CTCCAGAATT TGAGGGTTGC
    TTGGAATACA ACCATGAAGA GTGGATAGAG ATCAGAGTAG CATTTGAGGA TTTTATTAAT
    AATGCAACTC ATATGCTGTC TCGATTAGAG GAATTACAGG AGATGCTTGC AATTAAAGAG
    TTGCCTCAGG ATTTAGAGGG GGCACGCAAT ATGATTGAAG AACATTCTCA AATGAAAAAA
    AGGGTTATAA AGGCTCCTAT TGAAGATCTT GATTTAGAAG GGCAAAAGCT TTTACAAACA
    ATACAAAGCA GTGATAGCTT TCCTAAGAAG AACTCTAGTT CTGGAAATGC AGACCTTCAA
    AATCTTCTTC CCAAAGTTTC CACAATGTTA GACAGGCTTC ATTCAACACG ACAACATCTT
    CATCAGATGT GGCACATGCG AAAAGTAAAA CTGGACCAGT GCTTTCAGCT AAGGTTGTTT
    GAGCAGGATG CTGAGAAGAT GTTTGACTGG ATTACACACA ACAAAGGTCT GTTTCTGACA
    AGTTATACTG ACATTGGCAC CCGTAATAAT CATGCTATGG AACTTCAGAC ACAGCACAAT
    CATTTTGCTA TGAACTGCAT GAATGTCTAT GTAAACATTA GCCGAATAAT GTCTGTGGCA
    AATCGACTAG TAGAATCTGG ACACTATGCC TCACAGCAAA TTAAGCAGAT AGCTAACCAG
    TTGGAGCAAG AGTGGAAGGC TTTTGCCGCA GCCCTTGATG AACGTAGTAC GTTGCTTGAC
    ATGTCTGCAA TGTTCCATCA AAAAGCGGAG CAGTACATGG GCAATGTGGA TTCATGGTGC
    AAAGCTTGTG GGGAAGTGGA TCTTCCATCA GAGCTGCAGG ATCTAGAAGA TGCCATTCAT
    CACCATCAGG GAATATATGA GCATATCACT TTAGCATATT CAGAGGTTAG CCAGGATGGT
    AAATCTTTAT TAGACAAACT CCAGAGACCA TTGACTCCTG GTAGTTCTGA CTCACTGACA
    GCTTCTGCCA ACTATTCAAA GGCTGTGCAT CACGTCTTAG ATGCAATACA TGAAGTCTTG
    CACCACCAGC GTCAGTTGGA AAACATCTGG CAACATCGCA AAGTTCGTCT GCATCAACGC
    CTACAGCTCT GTGTTTTTCA GCAAGATGTT CAGCAGGTCT TGGATTGGAT AGAGAACCAT
    GGTGAAGCCT TTCTCAGCAA ACACACTGGG GTAGGCAAGT CTCTGCACAG GGCCAGAGCC
    TTGCAAAAGC GACATGAAGA CTTTGAAGAA GTCGCTCAGA ATACATACAC CAATGCTGAT
    AAGCTGCTGG AAGCTGCAGA ACAGTTGGCC CAGACAGGAG AGTGCGATCC AGAGGAAATA
    TACCAGGCTG CTCATCAGCT TGAGGATCGG ATTCAGGATT TTGTAAGACG TGTAGAACAA
    AGAAAAGTTC TCTTGGACAT GTCTGTATCA TTCCACTCGC ATGTTAAAGA GCTATGGACA
    TGGCTGGAGG ACCTACAAAA GGAACTGCTG GATGATGTCT ATGCGGAGTC AGTAGAAGCT
    GTACAGGACT TGATCAAACG GTTTGGACAA CAGCAGCAGA CAACCCTCCA GTTTACAGTA
    AATGTCATCA AAGAAGGGGA GGATCTAATT CAGCAACTAA GAGATTCTGC TATATCCAGC
    AACAAAACTC CTCACAACAG CTCAATCAAT CACATTGAGA CTGTCCTTCA ACAACTTGAT
    GAGGCCCAAT CACAGATGGA GGAGCTTTTC CAAGAGAGAA AAATCAAACT TGAGCTATTC
    TTGCAGCTTC GCATCTTTGA GAGAGATGCC ATTGATATCA TCTCAGACTT GGAATCATGG
    AATGATGAAC TTTCTCAGCA AATGAATGAT TTTGACACAG AGGACCTCAC ACTAGCCGAG
    CAAAGGCTAC AGCATCACGC AGACAAAGCT CTGACAATGA ACAACCTGAC ATTTGATGTC
    ATCCACCAAG GACAAGAGTT ACTACAATAT GTGAATGAAG TTCAGAACTC AGGGGTTGAG
    CTACTCTGTG ATAGAGATGT AGATATGGCT ACTAGAGTTC AGGACCTGTT GGAATTCCTC
    CATGAGAAAC AACAGGAGTT GGATCTAGCA GCTGAGCAGC ACCGAAAACA TCTAGAACAA
    TGTGTGCAAC TACGACATTT GCAAGCTGAA GTCAAACAGG TACTCGGCTG GATACGGAAT
    GGAGAGTCTA TGTTAAATGC CGGGCTTATC ACTGCCAGTT CATTGCAAGA AGCAGAGCAA
    CTTCAAAGGG AACATGAGCA GTTCCAGCAT GCAATAGAAA AAACCCACCA GAGTGCTCTG
    CAGGTGCAGC AGAAGGCAGA GGCCATGCTT CAAGCAAACC ATTACGACAT GGATATGATC
    CGGGACTGTG CTGAAAAAGT GGCCTCTCAT TGGCAACAGT TGATGCTTAA AATGGAAGAC
    CGTCTTAAGC TTGTCAATGC CTCTGTGGCC TTTTATAAAA CCTCAGAGCA GGTATGCAGT
    GTTCTAGAGA GCCTAGAGCA GGAGTACAAG CGGGAGGAAG ATTGGTGTGG CGGAGCTGAT
    AAACTTGGGC CTAACTCAGA GACTGACCAT GTTACTCCTA TGATCAGCAA ACACCTTGAA
    CAAAAGGAAG CCTTTCTTAA GGCCTGCACT TTGGCTCGCA GAAATGCTGA TGTCTTTCTG
    AAATACTTGC ACAGGAATAG TGTCAATATG CCTGGAATGG TGACACATGT AAAGGCTCCT
    GAACAGCAAG TAAAAAATAT TTTAAATGAG CTGTTTCAGA GGGAAAATAG AGTTCTTCAT
    TATTGGACCT TGCGGAAAAG GAGACTAGAC CATTGCCAGC AGTATGTAGT ATTTGAAAGA
    AGTGCCAAAC AGGCTCTGGA GTGGATCCAA GATAATGGAG AATTTTACAT TTCCACACAC
    ACCTCCACTG GCTCCACAAT TCAGCATACA CAAGAACTCT TAAAAGAGCA TGAGGAGTTT
    CAGATCACAG CAAAGCAAAC CAAAGAGAGG GTAAAGCTGT TGATACAGCT GGCTGATGGC
    TTTTGTGAAA AAGGGCATGC TCATGCTGCA GAAATTAAAA AATGTGTTAC AGCGGTGGAC
    AAGAGGTACA GAGACTTCTC GCTGCGTATG GAAAAGTACA GAACCAGCTT AGAGAAGGCT
    TTGGGCATTT CTTCTGATTC CAAGTCGAGT AAAAGCCTTC AGTTGGATAT TATTCCAGCC
    ACTGCCCCTG GTTCCGAAGT CAAGCTTCGT GATGCAACAC ATGAACTCAA TGAGGAGAAA
    AGAAAATCTG CTCGTCGAAA AGAATTCATC ATGGCAGAAC TTATACAAAC TGAAAAAGCA
    TATGTTCGTG ATCTTCGGGA ATGCATGGAT ACATATCTGT GGGAAATGAC AAGCGGAGTT
    GAAGAGATCC CACCAGGCAT TGTAAATAAA GAACACATTA TCTTTGGAAA CATGCAGGAA
    ATATATGAAT TTCATAACAA CATATTCTTA AAGGAGCTTG AGAAGTATGA GCAGCTACCT
    GAAGATGTTG GACATTGTTT TGTCACATGG GCTGACAAAT TTCAGATGTA TGTGTCCTAC
    TGTAAGAATA AGCCTGATTC AACACAACTT ATCTTGGAAC AAGCAGGAAC ATATTTTGAT
    GAAATTCAGC AGCGCCATGA GCTGGTCAAC TCCATCTCTT CTTATCTTAT CAAACCTGTG
    CAAAGGATAA CAAAATATCA GCTTCTTTTA AAAGAGCTGC TGACATGCTG CGAAGAAGGT
    AAAGGAGAGA TTAAAGATGG GCTGGAAGTG ATGCTAAGTG TACCAAAAAG AGCCAACGAT
    GCAATGCATC TCAGCATGCT TGAAGGGTTT GATGAGAACA TTGAATCTCA GGGAGAACTT
    ATTCTGCAAG AGTCTTTTCA AGTGTGGGAT CCTAAAACCT TAATAAGAAA GGGGCGTGAG
    AGACATCTCT TCTTGTTTGA AATGTCTTTG GTCTTCAGCA AAGAAGTGAA AGATTCAAGT
    GGAAGGAGCA AATATATTTA CAAATCAAAA TTATTTACCT CAGAGCTTGG AGTCACAGAA
    CATGTAGAAG GTGATCCTTG CAAATTTGCA CTTTGGGTAG GCAGAACGCC AACATCTGAT
    AACAAAATAG TCCTTAAGGG CTCAAGTATT GAGAACAAGC AAGATTGGAT CAAGCATATA
    AGAGAAGTGA TCCAAGACCG AACCATTCAC CTGAGAGGCG CATTAAAAGA GCCTATTCAT
    ATACCTAAAA CAGCTGCTGC TACAAAACAG AAACCTGCCA AAAGAGATGG AGAAGACCTG
    GACAGTCAAG GAGATGGCAG CAGCCAACCT GATACAATAT CTATTGCTTC ACGAACTTCA
    CAGAACACAT TAGACAGTGA TAAGCTTTCT GGTGGATGTG AGCTGACAGT AGTCATCCAT
    GACTTTACTG CCTGTAATAG CAATGAGCTG ACAATTAAAC GAGGGCAGAC TGTGGAGGTT
    CTAGAGCGGC ATCACGATCG ACCTGACTGG TGTTTAGTTC GGACTACTGA CCGGTCACCT
    GCAGCAGAGG GCTTGGTGCC TAGTAGTTCA CTGTGTATTG CACATTCTAG AAGTAGCATG
    GAAATGGAAG GCATATTTAA CCACAAAGAT TCCCTCTCTG TCTCCAGTAA TGATGCCAGT
    CCTCCTGCTT CAGTAATTTC CCTTCAGCCT CACATGATTG GTGCTCAAAG CTCTCCTGGC
    CCAAAGCGAC CTGGCAATAC TTTGAGAAAA TGGCTCACCA GCCCTGTCAG GAGACTTAGC
    AGTGGCAAAG CTGATGGACA TGTGAAAAAA TTGGCTCATA AACACAAAAA AAGCAGGGAG
    GTTCGTAAAA ACACAGACTC AGGTTCCCAA AAGGACTCTG ATGACAGTGC AGCAACACCT
    CAAGATGAAA CTATCGAGGA GAGAGTGCGC AATGAAGGAC TTAGCAGTGG TACGCTTTCT
    AAATCCTCCT CGTCTGGCAT GCAAAGCTGT GGAGAAGAAG AGGGCGAGGA AGGACCTGAT
    GCAGTTCCAT TGCCTCCACC CATGGCTATT CAGCAGCACA GCCTTCTTCA GCCTGACTCA
    CAGGATGACA AGACATCATC TCGGTTGCTG GTGCGACCTA CTAGCTCAGA GACACCTAGT
    GCTGCAGAGC TTGTTAGTGC TATTGAAGAA CTTGTAAAGA GTAAAATGGC TTTGGAAGAC
    CGGCCAAGTT CACTTCAAGT AGACCAGGGA GACAGTAGTA GCCCATCATT CAACCCTTCA
    GATACATCTC TGCTTTCCTC CTCTTCTCCA ATTGATGAAA TGGAAGAAAA AAAATCAAGC
    TCTTTAAAAA GAAGGCACTA TGTATTACAA GAGCTAGTAG AAACTGAACG CGATTATGTT
    CGGGACCTTG GCTATGTGGT TGAGGGTTAT ATGGCACTTA TGAAGGAGGA TGGAGTTCCA
    GATGATATGA AAGGAAAAGA CAAGATTGTT TTTGGCAACT TACACCAGAT CTACGAGTGG
    CACAGAGACT TTTTTTTGGG TGAACTGGAA AGGTGCCTGG ATGAGCCTGA AAGATTGGCA
    TCCCTATTTG TCAAGCATGA AAGAAGATTG CATATGTACA TAGTTTACTG CCAAAATAAA
    CCAAAGTCTG AACATATTGT TTCAGAATAC ATTGATACCT ATTTTGAGGA CCTGAAACAG
    CGTCTGGGCC ATCGACTTCA GCTTACAGAT CTGTTAATAA AACCCATACA AAGAATAATG
    AAATATCAGC TGCTATTAAA GGATTTTCTC AAGTACTCTA AGAAGGCTTG CATAGATGTA
    GCAGAACTTG AGAGAGCTGT GGAAGTCATG TGTATCGTAC CGAAGCGGTG TAATGACATG
    ATGAATGTTG GTCGTTTGCA AGGATTTGAT GGCAAAATAG TTGCCCAAGG AAAGCTGCTA
    CTGCAAGACA CATTCCTTGT GACTGAGCAG GACGCTGGTC TTCTGCCCCG ATGCAAAGAA
    AGGCGGGTCT TCCTTTTCGA ACAAATTGTC ATCTTTAGTG AGCCCTTAGA TAAAAAGAAA
    GGATACTCTA CACCTGGATT TCTTTTTAAA AATAATATCA AAGTGAGCTG CCTTAGTTTA
    GAGGAGTGTG TTGATAGTGA TCCATGCAAG TTTGCACTAT TGTCAAGGTC TGCTGACTCT
    ACAGAGATGT TTGTTTTGCA TTCTTCATCG CCTGGAGTGA GACAGTTATG GATTCATGAA
    ATCAACCAAA TTTTGGAAAA CCAGCGCAAT TTTTTAAATG CCTTGACATC ACCAATTGAA
    TACCACAGAA ATCACACTGG TGGAAGCAGT AGTGGCAACG TCACCTTTAC CAGCAGTCCC
    AGCAATTGTA GTGGGCTGGG TGGCTCCAGC CGAAGCAGGC CCTCTCGGAT TCCCCAGCCT
    GTTAGACACC ACTCTCCAGT CCTGGTCTCC TCTGCAGCTT CGGCACAGTC AGAGGCAGAC
    AAGATGTCAG GTATGTCAGC TCGCCGTGCA CCAGTTGTAC CTCTCAACCT CTCCTTAGAC
    AATAACCCCA GCCAGAAAGG TAGAGTAACT GCTGCTCCAG AGACAGGCGG GCGAGATAAA
    GAATCCAATT CAGTGCCAAA GATGAAAATG CTTGATGGCC CCAGGAAATC AGCTAGTGGC
    ATTCCAACCT TAGCTGATGG TGCACAGAAA GAATTGCGTG GGAGCACAGA AGACAGTCGA
    ATTAGAAGCA CTATGGGCAC TCTAACCTTA GGAAAGCCAA GAAATGGTTC TGCCTCACCC
    CTCAACTCTC CTGTCACAAT TAATTTTCCA TCGTCTTTTG GTAAGGAATC AGGGCCACCA
    AGCAGTCCCC TTCAGAAAGG CTCTTTTTGG AGCTCAATTC CAGGATCTCC AGCCAGCCGA
    CCTGGCTCCT TCACTTTCCC TGGAGACAGT GACTCTCTCC AGCGACAAGT TCATCGGCAC
    TCCACACACA GCAAGGATAC AGACCGCATG AGCACCTGCT CCTCGACTAG TGAGCAGTCC
    GTTCACTCCA CTCAGAGTAA TGGGGTAAGG ACAGAGAGTG AAAGTAGCAG CAGTAGCAAT
    ATCTCCACCA TGCTGGTTAC GCACGATTAT ACAGCGGTGA AGGAGGATGA GATCAATGTC
    TACCAGGGAG AGGTTGTACA AATTCTGGCA AGTAACCAAC AGAACATGTT TCTGGTGTTC
    AGAGCTGCCA CTGATGAGTG CCCTGCAGCT GAGGGCTGGA TTCCAAGCTA TGTTCTGGGT
    CACACAAGTG CAATCATCAC TGATATGACT GATGCAACTA TGAAGAAGTC CTCTTCTTGG
    CATACAGCCC TTAGAATAAG AAAGAAATCT GAGAAGAGGG AAAAAGATGG CAGCAGAGAC
    ACAAAACTAG AGAATGGTTA TCGCAAATCA AGAGAAGGAC TAGCTAATAA GCTCCTGAAT
    CCAAACTACA TTTATGATGC TCCTCCAGAG TTTATTGTAC CACTGAACGA GGTTACATGT
    GACACAGGAG AGACTGTGAT TATTCGATGC AAAGTTTGTG GTCGCCCCAA GGCATCCATT
    ACATGGAAAG GTCCCGACCA TAATACTTTG AGCAACGATG GACACTACAG TATATCCTAC
    AGTGATCTTG GAGAAGCAAC TCTAAAAATA GTTGGTGTGA CCACTGAGGA TGATGGTATA
    TACACCTGCA TTGCAACAAA TGACTTGGGT TCAGTATCCG CTTCCTCCAA TCTTAGAGTG
    TTAGGTTCAT GGAGTGATGG ACTGAGAGTA ATCTGGAAAG AAAACTTCGA CATTGCTTAT
    AGTGAAGTGG CTGAACTTGG CAGGGGCAGG TTTTCTGTCG TGAAAAAATG TGAACAAAGA
    GTTACAAGAC GCATAGTAGC CACCAAATTT GTTAACAAGA AGTTGATGAA AAGAGACCAG
    GTTACCCATG AACTTGGAAT TCTGCAGAGC CTTCAGCACC CACAACTGAT CAGCCTTCTT
    GACACTTTTG AGACACCAGC AAGCTATGTC TTAGTACTTG AGATAGCTGA TCAGGGATGT
    TTGTTAGAAT ATGTGGTACA GTGGGGAAAC CTTACAGAAG AAAAGATCAC CTCATATATG
    AGAGAGGTGC TGGAGGCCCT GCAGTATCTT CATAATTGCA GAATAGCACA TCTGGACCTA
    AAGCCTGAGA ACATTCTTGT GGATCAGAGC CTAACCAAAC CAACTATAAA ACTGTCAGAC
    TTTGGCGATG CTGTTCAGCT TAATACCACT TACTACATTC ACCAATTGTT GGGCAGTGCA
    GAGTTTGCCG CTCCAGAGAT CATCCTTGGG AATCCTGTCT CCCTTACCTC TGATATATGG
    AGTATTGGAG TTCTTACTTA TGTCCTTCTC AGTGGCGTCT CTCCTTTCCT TGATGAAAGT
    GTTGAAGAGA CATGCCTGAA CATTTGCCGA CTAGACTTTA GTTTTCCCGA TGACTACTTT
    AAGGGAGTAA GTCAGAAGGC CAAAGAATTT GTATGTTTCT TACTGCAGGG TGACCAGACA
    AAGCGCCCTT CTGCTGCTGT ATGTCTAAAG GAACAGTGGT TGCAGCCAGG CCAGCACAAA
    CGTAGTGGCA CATTAGACAT GTCAAGACTG ACCTCGTTTA TTGAACGCCG TAAACACCAG
    AATGATGTTC GACCCATTGG TAGCATTAAA AACTTCCTTC AGAGTCGCCT TTTACCCAGA
    ATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32255
    Clone ID Related Accession (Same CDS sequence) XM_012964355.2 , XM_012964355.3
    Accession Version XM_012964355.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9246bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product triple functional domain protein isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012964355.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGAGCACAT CCAGCACCGC AAGCTCCGGT AGCGCAAATA CCGGTACCGG CAATGCCGTT 
    GGAAGCAGCG GAGGCAACAC CGGGGAGGGG GCGGAGGAGA CGGCCAAGGA CTCTGCAGAC
    ATAGCTGCCT TTTACAGATC CGGTTTTCGT AGAAATGATG AATTGAAAGC AATGGAGGTG
    TTGCCAATTT TAAAAGAAAA AGTGGCATAT CTATCAGGTG GCAGAGATAA ACGAGGTGGT
    CCTGTATTAA CATTTCCAGC ACGCAGCAAC CATGATCGAA TACGACAAGA GGATCTCCGA
    AGACTGATTT CATATCTTGC TTGTATTCCT AGTGAGGAGG TGTGCAAGCG AGGCTTCACA
    GTGATTGTAG ATATGCGTGG TTCCAAGTGG GATTCTATTA AACCCTTGCT GAAAATTTTA
    CAAGAATCCT TTCCATGCTG TATTCATGTT GCACTAATTA TCAAACCAGA TAACTTCTGG
    CAGAAGCAAA GAACTAATTT TGGAAGTTCT AAGTTTGAAT TTGAGACAAG TATGGTGTCT
    TTAGAAGGCC TTACCAAAGT AGTTGACCCT TCCCAGCTAA CTCCAGAATT TGAGGGTTGC
    TTGGAATACA ACCATGAAGA GTGGATAGAG ATCAGAGTAG CATTTGAGGA TTTTATTAAT
    AATGCAACTC ATATGCTGTC TCGATTAGAG GAATTACAGG AGATGCTTGC AATTAAAGAG
    TTGCCTCAGG ATTTAGAGGG GGCACGCAAT ATGATTGAAG AACATTCTCA AATGAAAAAA
    AGGGTTATAA AGGCTCCTAT TGAAGATCTT GATTTAGAAG GGCAAAAGCT TTTACAAACA
    ATACAAAGCA GTGATAGCTT TCCTAAGAAG AACTCTAGTT CTGGAAATGC AGACCTTCAA
    AATCTTCTTC CCAAAGTTTC CACAATGTTA GACAGGCTTC ATTCAACACG ACAACATCTT
    CATCAGATGT GGCACATGCG AAAAGTAAAA CTGGACCAGT GCTTTCAGCT AAGGTTGTTT
    GAGCAGGATG CTGAGAAGAT GTTTGACTGG ATTACACACA ACAAAGGTCT GTTTCTGACA
    AGTTATACTG ACATTGGCAC CCGTAATAAT CATGCTATGG AACTTCAGAC ACAGCACAAT
    CATTTTGCTA TGAACTGCAT GAATGTCTAT GTAAACATTA GCCGAATAAT GTCTGTGGCA
    AATCGACTAG TAGAATCTGG ACACTATGCC TCACAGCAAA TTAAGCAGAT AGCTAACCAG
    TTGGAGCAAG AGTGGAAGGC TTTTGCCGCA GCCCTTGATG AACGTAGTAC GTTGCTTGAC
    ATGTCTGCAA TGTTCCATCA AAAAGCGGAG CAGTACATGG GCAATGTGGA TTCATGGTGC
    AAAGCTTGTG GGGAAGTGGA TCTTCCATCA GAGCTGCAGG ATCTAGAAGA TGCCATTCAT
    CACCATCAGG GAATATATGA GCATATCACT TTAGCATATT CAGAGGTTAG CCAGGATGGT
    AAATCTTTAT TAGACAAACT CCAGAGACCA TTGACTCCTG GTAGTTCTGA CTCACTGACA
    GCTTCTGCCA ACTATTCAAA GGCTGTGCAT CACGTCTTAG ATGCAATACA TGAAGTCTTG
    CACCACCAGC GTCAGTTGGA AAACATCTGG CAACATCGCA AAGTTCGTCT GCATCAACGC
    CTACAGCTCT GTGTTTTTCA GCAAGATGTT CAGCAGGTCT TGGATTGGAT AGAGAACCAT
    GGTGAAGCCT TTCTCAGCAA ACACACTGGG GTAGGCAAGT CTCTGCACAG GGCCAGAGCC
    TTGCAAAAGC GACATGAAGA CTTTGAAGAA GTCGCTCAGA ATACATACAC CAATGCTGAT
    AAGCTGCTGG AAGCTGCAGA ACAGTTGGCC CAGACAGGAG AGTGCGATCC AGAGGAAATA
    TACCAGGCTG CTCATCAGCT TGAGGATCGG ATTCAGGATT TTGTAAGACG TGTAGAACAA
    AGAAAAGTTC TCTTGGACAT GTCTGTATCA TTCCACTCGC ATGTTAAAGA GCTATGGACA
    TGGCTGGAGG ACCTACAAAA GGAACTGCTG GATGATGTCT ATGCGGAGTC AGTAGAAGCT
    GTACAGGACT TGATCAAACG GTTTGGACAA CAGCAGCAGA CAACCCTCCA GTTTACAGTA
    AATGTCATCA AAGAAGGGGA GGATCTAATT CAGCAACTAA GAGATTCTGC TATATCCAGC
    AACAAAACTC CTCACAACAG CTCAATCAAT CACATTGAGA CTGTCCTTCA ACAACTTGAT
    GAGGCCCAAT CACAGATGGA GGAGCTTTTC CAAGAGAGAA AAATCAAACT TGAGCTATTC
    TTGCAGCTTC GCATCTTTGA GAGAGATGCC ATTGATATCA TCTCAGACTT GGAATCATGG
    AATGATGAAC TTTCTCAGCA AATGAATGAT TTTGACACAG AGGACCTCAC ACTAGCCGAG
    CAAAGGCTAC AGCATCACGC AGACAAAGCT CTGACAATGA ACAACCTGAC ATTTGATGTC
    ATCCACCAAG GACAAGAGTT ACTACAATAT GTGAATGAAG TTCAGAACTC AGGGGTTGAG
    CTACTCTGTG ATAGAGATGT AGATATGGCT ACTAGAGTTC AGGACCTGTT GGAATTCCTC
    CATGAGAAAC AACAGGAGTT GGATCTAGCA GCTGAGCAGC ACCGAAAACA TCTAGAACAA
    TGTGTGCAAC TACGACATTT GCAAGCTGAA GTCAAACAGG TACTCGGCTG GATACGGAAT
    GGAGAGTCTA TGTTAAATGC CGGGCTTATC ACTGCCAGTT CATTGCAAGA AGCAGAGCAA
    CTTCAAAGGG AACATGAGCA GTTCCAGCAT GCAATAGAAA AAACCCACCA GAGTGCTCTG
    CAGGTGCAGC AGAAGGCAGA GGCCATGCTT CAAGCAAACC ATTACGACAT GGATATGATC
    CGGGACTGTG CTGAAAAAGT GGCCTCTCAT TGGCAACAGT TGATGCTTAA AATGGAAGAC
    CGTCTTAAGC TTGTCAATGC CTCTGTGGCC TTTTATAAAA CCTCAGAGCA GGTATGCAGT
    GTTCTAGAGA GCCTAGAGCA GGAGTACAAG CGGGAGGAAG ATTGGTGTGG CGGAGCTGAT
    AAACTTGGGC CTAACTCAGA GACTGACCAT GTTACTCCTA TGATCAGCAA ACACCTTGAA
    CAAAAGGAAG CCTTTCTTAA GGCCTGCACT TTGGCTCGCA GAAATGCTGA TGTCTTTCTG
    AAATACTTGC ACAGGAATAG TGTCAATATG CCTGGAATGG TGACACATGT AAAGGCTCCT
    GAACAGCAAG TAAAAAATAT TTTAAATGAG CTGTTTCAGA GGGAAAATAG AGTTCTTCAT
    TATTGGACCT TGCGGAAAAG GAGACTAGAC CATTGCCAGC AGTATGTAGT ATTTGAAAGA
    AGTGCCAAAC AGGCTCTGGA GTGGATCCAA GATAATGGAG AATTTTACAT TTCCACACAC
    ACCTCCACTG GCTCCACAAT TCAGCATACA CAAGAACTCT TAAAAGAGCA TGAGGAGTTT
    CAGATCACAG CAAAGCAAAC CAAAGAGAGG GTAAAGCTGT TGATACAGCT GGCTGATGGC
    TTTTGTGAAA AAGGGCATGC TCATGCTGCA GAAATTAAAA AATGTGTTAC AGCGGTGGAC
    AAGAGGTACA GAGACTTCTC GCTGCGTATG GAAAAGTACA GAACCAGCTT AGAGAAGGCT
    TTGGGCATTT CTTCTGATTC CAAGTCGAGT AAAAGCCTTC AGTTGGATAT TATTCCAGCC
    ACTGCCCCTG GTTCCGAAGT CAAGCTTCGT GATGCAACAC ATGAACTCAA TGAGGAGAAA
    AGAAAATCTG CTCGTCGAAA AGAATTCATC ATGGCAGAAC TTATACAAAC TGAAAAAGCA
    TATGTTCGTG ATCTTCGGGA ATGCATGGAT ACATATCTGT GGGAAATGAC AAGCGGAGTT
    GAAGAGATCC CACCAGGCAT TGTAAATAAA GAACACATTA TCTTTGGAAA CATGCAGGAA
    ATATATGAAT TTCATAACAA CATATTCTTA AAGGAGCTTG AGAAGTATGA GCAGCTACCT
    GAAGATGTTG GACATTGTTT TGTCACATGG GCTGACAAAT TTCAGATGTA TGTGTCCTAC
    TGTAAGAATA AGCCTGATTC AACACAACTT ATCTTGGAAC AAGCAGGAAC ATATTTTGAT
    GAAATTCAGC AGCGCCATGA GCTGGTCAAC TCCATCTCTT CTTATCTTAT CAAACCTGTG
    CAAAGGATAA CAAAATATCA GCTTCTTTTA AAAGAGCTGC TGACATGCTG CGAAGAAGGT
    AAAGGAGAGA TTAAAGATGG GCTGGAAGTG ATGCTAAGTG TACCAAAAAG AGCCAACGAT
    GCAATGCATC TCAGCATGCT TGAAGGGTTT GATGAGAACA TTGAATCTCA GGGAGAACTT
    ATTCTGCAAG AGTCTTTTCA AGTGTGGGAT CCTAAAACCT TAATAAGAAA GGGGCGTGAG
    AGACATCTCT TCTTGTTTGA AATGTCTTTG GTCTTCAGCA AAGAAGTGAA AGATTCAAGT
    GGAAGGAGCA AATATATTTA CAAATCAAAA TTATTTACCT CAGAGCTTGG AGTCACAGAA
    CATGTAGAAG GTGATCCTTG CAAATTTGCA CTTTGGGTAG GCAGAACGCC AACATCTGAT
    AACAAAATAG TCCTTAAGGG CTCAAGTATT GAGAACAAGC AAGATTGGAT CAAGCATATA
    AGAGAAGTGA TCCAAGACCG AACCATTCAC CTGAGAGGCG CATTAAAAGA GCCTATTCAT
    ATACCTAAAA CAGCTGCTGC TACAAAACAG AAACCTGCCA AAAGAGATGG AGAAGACCTG
    GACAGTCAAG GAGATGGCAG CAGCCAACCT GATACAATAT CTATTGCTTC ACGAACTTCA
    CAGAACACAT TAGACAGTGA TAAGCTTTCT GGTGGATGTG AGCTGACAGT AGTCATCCAT
    GACTTTACTG CCTGTAATAG CAATGAGCTG ACAATTAAAC GAGGGCAGAC TGTGGAGGTT
    CTAGAGCGGC ATCACGATCG ACCTGACTGG TGTTTAGTTC GGACTACTGA CCGGTCACCT
    GCAGCAGAGG GCTTGGTGCC TAGTAGTTCA CTGTGTATTG CACATTCTAG AAGTAGCATG
    GAAATGGAAG GCATATTTAA CCACAAAGAT TCCCTCTCTG TCTCCAGTAA TGATGCCAGT
    CCTCCTGCTT CAGTAATTTC CCTTCAGCCT CACATGATTG GTGCTCAAAG CTCTCCTGGC
    CCAAAGCGAC CTGGCAATAC TTTGAGAAAA TGGCTCACCA GCCCTGTCAG GAGACTTAGC
    AGTGGCAAAG CTGATGGACA TGTGAAAAAA TTGGCTCATA AACACAAAAA AAGCAGGGAG
    GTTCGTAAAA ACACAGACTC AGGTTCCCAA AAGGACTCTG ATGACAGTGC AGCAACACCT
    CAAGATGAAA CTATCGAGGA GAGAGTGCGC AATGAAGGAC TTAGCAGTGG TACGCTTTCT
    AAATCCTCCT CGTCTGGCAT GCAAAGCTGT GGAGAAGAAG AGGGCGAGGA AGGACCTGAT
    GCAGTTCCAT TGCCTCCACC CATGGCTATT CAGCAGCACA GCCTTCTTCA GCCTGACTCA
    CAGGATGACA AGACATCATC TCGGTTGCTG GTGCGACCTA CTAGCTCAGA GACACCTAGT
    GCTGCAGAGC TTGTTAGTGC TATTGAAGAA CTTGTAAAGA GTAAAATGGC TTTGGAAGAC
    CGGCCAAGTT CACTTCAAGT AGACCAGGGA GACAGTAGTA GCCCATCATT CAACCCTTCA
    GATACATCTC TGCTTTCCTC CTCTTCTCCA ATTGATGAAA TGGAAGAAAA AAAATCAAGC
    TCTTTAAAAA GAAGGCACTA TGTATTACAA GAGCTAGTAG AAACTGAACG CGATTATGTT
    CGGGACCTTG GCTATGTGGT TGAGGGTTAT ATGGCACTTA TGAAGGAGGA TGGAGTTCCA
    GATGATATGA AAGGAAAAGA CAAGATTGTT TTTGGCAACT TACACCAGAT CTACGAGTGG
    CACAGAGACT TTTTTTTGGG TGAACTGGAA AGGTGCCTGG ATGAGCCTGA AAGATTGGCA
    TCCCTATTTG TCAAGCATGA AAGAAGATTG CATATGTACA TAGTTTACTG CCAAAATAAA
    CCAAAGTCTG AACATATTGT TTCAGAATAC ATTGATACCT ATTTTGAGGA CCTGAAACAG
    CGTCTGGGCC ATCGACTTCA GCTTACAGAT CTGTTAATAA AACCCATACA AAGAATAATG
    AAATATCAGC TGCTATTAAA GGATTTTCTC AAGTACTCTA AGAAGGCTTG CATAGATGTA
    GCAGAACTTG AGAGAGCTGT GGAAGTCATG TGTATCGTAC CGAAGCGGTG TAATGACATG
    ATGAATGTTG GTCGTTTGCA AGGATTTGAT GGCAAAATAG TTGCCCAAGG AAAGCTGCTA
    CTGCAAGACA CATTCCTTGT GACTGAGCAG GACGCTGGTC TTCTGCCCCG ATGCAAAGAA
    AGGCGGGTCT TCCTTTTCGA ACAAATTGTC ATCTTTAGTG AGCCCTTAGA TAAAAAGAAA
    GGATACTCTA CACCTGGATT TCTTTTTAAA AATAATATCA AAGTGAGCTG CCTTAGTTTA
    GAGGAGTGTG TTGATAGTGA TCCATGCAAG TTTGCACTAT TGTCAAGGTC TGCTGACTCT
    ACAGAGATGT TTGTTTTGCA TTCTTCATCG CCTGGAGTGA GACAGTTATG GATTCATGAA
    ATCAACCAAA TTTTGGAAAA CCAGCGCAAT TTTTTAAATG CCTTGACATC ACCAATTGAA
    TACCACAGAA ATCACACTGG TGGAAGCAGT AGTGGCAACG TCACCTTTAC CAGCAGTCCC
    AGCAATTGTA GTGGGCTGGG TGGCTCCAGC CGAAGCAGGC CCTCTCGGAT TCCCCAGCCT
    GTTAGACACC ACTCTCCAGT CCTGGTCTCC TCTGCAGCTT CGGCACAGTC AGAGGCAGAC
    AAGATGTCAG GTATGTCAGC TCGCCGTGCA CCAGTTGTAC CTCTCAACCT CTCCTTAGAC
    AATAACCCCA GCCAGAAAGG TAGAGTAACT GCTGCTCCAG AGACAGGCGG GCGAGATAAA
    GAATCCAATT CAGTGCCAAA GATGAAAATG CTTGATGGCC CCAGGAAATC AGCTAGTGGC
    ATTCCAACCT TAGCTGATGG TGCACAGAAA GAATTGCGTG GGAGCACAGA AGACAGTCGA
    ATTAGAAGCA CTATGGGCAC TCTAACCTTA GGAAAGCCAA GAAATGGTTC TGCCTCACCC
    CTCAACTCTC CTGTCACAAT TAATTTTCCA TCGTCTTTTG GTAAGGAATC AGGGCCACCA
    AGCAGTCCCC TTCAGAAAGG CTCTTTTTGG AGCTCAATTC CAGGATCTCC AGCCAGCCGA
    CCTGGCTCCT TCACTTTCCC TGGAGACAGT GACTCTCTCC AGCGACAAGT TCATCGGCAC
    TCCACACACA GCAAGGATAC AGACCGCATG AGCACCTGCT CCTCGACTAG TGAGCAGTCC
    GTTCACTCCA CTCAGAGTAA TGGGGTAAGG ACAGAGAGTG AAAGTAGCAG CAGTAGCAAT
    ATCTCCACCA TGCTGGTTAC GCACGATTAT ACAGCGGTGA AGGAGGATGA GATCAATGTC
    TACCAGGGAG AGGTTGTACA AATTCTGGCA AGTAACCAAC AGAACATGTT TCTGGTGTTC
    AGAGCTGCCA CTGATGAGTG CCCTGCAGCT GAGGGCTGGA TTCCAAGCTA TGTTCTGGGT
    CACACAAGTG CAATCATCAC TGATATGACT GATGCAACTA TGAAGAAGTC CTCTTCTTGG
    CATACAGCCC TTAGAATAAG AAAGAAATCT GAGAAGAGGG AAAAAGATGG CAGCAGAGAC
    ACAAAACTAG AGAATGGTTA TCGCAAATCA AGAGAAGGAC TAGCTAATAA GCTCCTGAAT
    CCAAACTACA TTTATGATGC TCCTCCAGAG TTTATTGTAC CACTGAACGA GGTTACATGT
    GACACAGGAG AGACTGTGAT TATTCGATGC AAAGTTTGTG GTCGCCCCAA GGCATCCATT
    ACATGGAAAG GTCCCGACCA TAATACTTTG AGCAACGATG GACACTACAG TATATCCTAC
    AGTGATCTTG GAGAAGCAAC TCTAAAAATA GTTGGTGTGA CCACTGAGGA TGATGGTATA
    TACACCTGCA TTGCAACAAA TGACTTGGGT TCAGTATCCG CTTCCTCCAA TCTTAGAGTG
    TTAGGTTCAT GGAGTGATGG ACTGAGAGTA ATCTGGAAAG AAAACTTCGA CATTGCTTAT
    AGTGAAGTGG CTGAACTTGG CAGGGGCAGG TTTTCTGTCG TGAAAAAATG TGAACAAAGA
    GTTACAAGAC GCATAGTAGC CACCAAATTT GTTAACAAGA AGTTGATGAA AAGAGACCAG
    GTTACCCATG AACTTGGAAT TCTGCAGAGC CTTCAGCACC CACAACTGAT CAGCCTTCTT
    GACACTTTTG AGACACCAGC AAGCTATGTC TTAGTACTTG AGATAGCTGA TCAGGGATGT
    TTGTTAGAAT ATGTGGTACA GTGGGGAAAC CTTACAGAAG AAAAGATCAC CTCATATATG
    AGAGAGGTGC TGGAGGCCCT GCAGTATCTT CATAATTGCA GAATAGCACA TCTGGACCTA
    AAGCCTGAGA ACATTCTTGT GGATCAGAGC CTAACCAAAC CAACTATAAA ACTGTCAGAC
    TTTGGCGATG CTGTTCAGCT TAATACCACT TACTACATTC ACCAATTGTT GGGCAGTGCA
    GAGTTTGCCG CTCCAGAGAT CATCCTTGGG AATCCTGTCT CCCTTACCTC TGATATATGG
    AGTATTGGAG TTCTTACTTA TGTCCTTCTC AGTGGCGTCT CTCCTTTCCT TGATGAAAGT
    GTTGAAGAGA CATGCCTGAA CATTTGCCGA CTAGACTTTA GTTTTCCCGA TGACTACTTT
    AAGGGAGTAA GTCAGAAGGC CAAAGAATTT GTATGTTTCT TACTGCAGGG TGACCAGACA
    AAGCGCCCTT CTGCTGCTGT ATGTCTAAAG GAACAGTGGT TGCAGCCAGG CCAGCACAAA
    CGTAGTGGCA CATTAGACAT GTCAAGACTG ACCTCGTTTA TTGAACGCCG TAAACACCAG
    AATGATGTTC GACCCATTGG TAGCATTAAA AACTTCCTTC AGAGTCGCCT TTTACCCAGA
    ATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012819809.1
    CDS527..9772
    Translation

    Target ORF information:

    RefSeq Version XM_012964355.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012964355.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGAGCACAT CCAGCACCGC AAGCTCCGGT AGCGCAAATA CCGGTACCGG CAATGCCGTT 
    GGAAGCAGCG GAGGCAACAC CGGGGAGGGG GCGGAGGAGA CGGCCAAGGA CTCTGCAGAC
    ATAGCTGCCT TTTACAGATC CGGTTTTCGT AGAAATGATG AATTGAAAGC AATGGAGGTG
    TTGCCAATTT TAAAAGAAAA AGTGGCATAT CTATCAGGTG GCAGAGATAA ACGAGGTGGT
    CCTGTATTAA CATTTCCAGC ACGCAGCAAC CATGATCGAA TACGACAAGA GGATCTCCGA
    AGACTGATTT CATATCTTGC TTGTATTCCT AGTGAGGAGG TGTGCAAGCG AGGCTTCACA
    GTGATTGTAG ATATGCGTGG TTCCAAGTGG GATTCTATTA AACCCTTGCT GAAAATTTTA
    CAAGAATCCT TTCCATGCTG TATTCATGTT GCACTAATTA TCAAACCAGA TAACTTCTGG
    CAGAAGCAAA GAACTAATTT TGGAAGTTCT AAGTTTGAAT TTGAGACAAG TATGGTGTCT
    TTAGAAGGCC TTACCAAAGT AGTTGACCCT TCCCAGCTAA CTCCAGAATT TGAGGGTTGC
    TTGGAATACA ACCATGAAGA GTGGATAGAG ATCAGAGTAG CATTTGAGGA TTTTATTAAT
    AATGCAACTC ATATGCTGTC TCGATTAGAG GAATTACAGG AGATGCTTGC AATTAAAGAG
    TTGCCTCAGG ATTTAGAGGG GGCACGCAAT ATGATTGAAG AACATTCTCA AATGAAAAAA
    AGGGTTATAA AGGCTCCTAT TGAAGATCTT GATTTAGAAG GGCAAAAGCT TTTACAAACA
    ATACAAAGCA GTGATAGCTT TCCTAAGAAG AACTCTAGTT CTGGAAATGC AGACCTTCAA
    AATCTTCTTC CCAAAGTTTC CACAATGTTA GACAGGCTTC ATTCAACACG ACAACATCTT
    CATCAGATGT GGCACATGCG AAAAGTAAAA CTGGACCAGT GCTTTCAGCT AAGGTTGTTT
    GAGCAGGATG CTGAGAAGAT GTTTGACTGG ATTACACACA ACAAAGGTCT GTTTCTGACA
    AGTTATACTG ACATTGGCAC CCGTAATAAT CATGCTATGG AACTTCAGAC ACAGCACAAT
    CATTTTGCTA TGAACTGCAT GAATGTCTAT GTAAACATTA GCCGAATAAT GTCTGTGGCA
    AATCGACTAG TAGAATCTGG ACACTATGCC TCACAGCAAA TTAAGCAGAT AGCTAACCAG
    TTGGAGCAAG AGTGGAAGGC TTTTGCCGCA GCCCTTGATG AACGTAGTAC GTTGCTTGAC
    ATGTCTGCAA TGTTCCATCA AAAAGCGGAG CAGTACATGG GCAATGTGGA TTCATGGTGC
    AAAGCTTGTG GGGAAGTGGA TCTTCCATCA GAGCTGCAGG ATCTAGAAGA TGCCATTCAT
    CACCATCAGG GAATATATGA GCATATCACT TTAGCATATT CAGAGGTTAG CCAGGATGGT
    AAATCTTTAT TAGACAAACT CCAGAGACCA TTGACTCCTG GTAGTTCTGA CTCACTGACA
    GCTTCTGCCA ACTATTCAAA GGCTGTGCAT CACGTCTTAG ATGCAATACA TGAAGTCTTG
    CACCACCAGC GTCAGTTGGA AAACATCTGG CAACATCGCA AAGTTCGTCT GCATCAACGC
    CTACAGCTCT GTGTTTTTCA GCAAGATGTT CAGCAGGTCT TGGATTGGAT AGAGAACCAT
    GGTGAAGCCT TTCTCAGCAA ACACACTGGG GTAGGCAAGT CTCTGCACAG GGCCAGAGCC
    TTGCAAAAGC GACATGAAGA CTTTGAAGAA GTCGCTCAGA ATACATACAC CAATGCTGAT
    AAGCTGCTGG AAGCTGCAGA ACAGTTGGCC CAGACAGGAG AGTGCGATCC AGAGGAAATA
    TACCAGGCTG CTCATCAGCT TGAGGATCGG ATTCAGGATT TTGTAAGACG TGTAGAACAA
    AGAAAAGTTC TCTTGGACAT GTCTGTATCA TTCCACTCGC ATGTTAAAGA GCTATGGACA
    TGGCTGGAGG ACCTACAAAA GGAACTGCTG GATGATGTCT ATGCGGAGTC AGTAGAAGCT
    GTACAGGACT TGATCAAACG GTTTGGACAA CAGCAGCAGA CAACCCTCCA GTTTACAGTA
    AATGTCATCA AAGAAGGGGA GGATCTAATT CAGCAACTAA GAGATTCTGC TATATCCAGC
    AACAAAACTC CTCACAACAG CTCAATCAAT CACATTGAGA CTGTCCTTCA ACAACTTGAT
    GAGGCCCAAT CACAGATGGA GGAGCTTTTC CAAGAGAGAA AAATCAAACT TGAGCTATTC
    TTGCAGCTTC GCATCTTTGA GAGAGATGCC ATTGATATCA TCTCAGACTT GGAATCATGG
    AATGATGAAC TTTCTCAGCA AATGAATGAT TTTGACACAG AGGACCTCAC ACTAGCCGAG
    CAAAGGCTAC AGCATCACGC AGACAAAGCT CTGACAATGA ACAACCTGAC ATTTGATGTC
    ATCCACCAAG GACAAGAGTT ACTACAATAT GTGAATGAAG TTCAGAACTC AGGGGTTGAG
    CTACTCTGTG ATAGAGATGT AGATATGGCT ACTAGAGTTC AGGACCTGTT GGAATTCCTC
    CATGAGAAAC AACAGGAGTT GGATCTAGCA GCTGAGCAGC ACCGAAAACA TCTAGAACAA
    TGTGTGCAAC TACGACATTT GCAAGCTGAA GTCAAACAGG TACTCGGCTG GATACGGAAT
    GGAGAGTCTA TGTTAAATGC CGGGCTTATC ACTGCCAGTT CATTGCAAGA AGCAGAGCAA
    CTTCAAAGGG AACATGAGCA GTTCCAGCAT GCAATAGAAA AAACCCACCA GAGTGCTCTG
    CAGGTGCAGC AGAAGGCAGA GGCCATGCTT CAAGCAAACC ATTACGACAT GGATATGATC
    CGGGACTGTG CTGAAAAAGT GGCCTCTCAT TGGCAACAGT TGATGCTTAA AATGGAAGAC
    CGTCTTAAGC TTGTCAATGC CTCTGTGGCC TTTTATAAAA CCTCAGAGCA GGTATGCAGT
    GTTCTAGAGA GCCTAGAGCA GGAGTACAAG CGGGAGGAAG ATTGGTGTGG CGGAGCTGAT
    AAACTTGGGC CTAACTCAGA GACTGACCAT GTTACTCCTA TGATCAGCAA ACACCTTGAA
    CAAAAGGAAG CCTTTCTTAA GGCCTGCACT TTGGCTCGCA GAAATGCTGA TGTCTTTCTG
    AAATACTTGC ACAGGAATAG TGTCAATATG CCTGGAATGG TGACACATGT AAAGGCTCCT
    GAACAGCAAG TAAAAAATAT TTTAAATGAG CTGTTTCAGA GGGAAAATAG AGTTCTTCAT
    TATTGGACCT TGCGGAAAAG GAGACTAGAC CATTGCCAGC AGTATGTAGT ATTTGAAAGA
    AGTGCCAAAC AGGCTCTGGA GTGGATCCAA GATAATGGAG AATTTTACAT TTCCACACAC
    ACCTCCACTG GCTCCACAAT TCAGCATACA CAAGAACTCT TAAAAGAGCA TGAGGAGTTT
    CAGATCACAG CAAAGCAAAC CAAAGAGAGG GTAAAGCTGT TGATACAGCT GGCTGATGGC
    TTTTGTGAAA AAGGGCATGC TCATGCTGCA GAAATTAAAA AATGTGTTAC AGCGGTGGAC
    AAGAGGTACA GAGACTTCTC GCTGCGTATG GAAAAGTACA GAACCAGCTT AGAGAAGGCT
    TTGGGCATTT CTTCTGATTC CAAGTCGAGT AAAAGCCTTC AGTTGGATAT TATTCCAGCC
    ACTGCCCCTG GTTCCGAAGT CAAGCTTCGT GATGCAACAC ATGAACTCAA TGAGGAGAAA
    AGAAAATCTG CTCGTCGAAA AGAATTCATC ATGGCAGAAC TTATACAAAC TGAAAAAGCA
    TATGTTCGTG ATCTTCGGGA ATGCATGGAT ACATATCTGT GGGAAATGAC AAGCGGAGTT
    GAAGAGATCC CACCAGGCAT TGTAAATAAA GAACACATTA TCTTTGGAAA CATGCAGGAA
    ATATATGAAT TTCATAACAA CATATTCTTA AAGGAGCTTG AGAAGTATGA GCAGCTACCT
    GAAGATGTTG GACATTGTTT TGTCACATGG GCTGACAAAT TTCAGATGTA TGTGTCCTAC
    TGTAAGAATA AGCCTGATTC AACACAACTT ATCTTGGAAC AAGCAGGAAC ATATTTTGAT
    GAAATTCAGC AGCGCCATGA GCTGGTCAAC TCCATCTCTT CTTATCTTAT CAAACCTGTG
    CAAAGGATAA CAAAATATCA GCTTCTTTTA AAAGAGCTGC TGACATGCTG CGAAGAAGGT
    AAAGGAGAGA TTAAAGATGG GCTGGAAGTG ATGCTAAGTG TACCAAAAAG AGCCAACGAT
    GCAATGCATC TCAGCATGCT TGAAGGGTTT GATGAGAACA TTGAATCTCA GGGAGAACTT
    ATTCTGCAAG AGTCTTTTCA AGTGTGGGAT CCTAAAACCT TAATAAGAAA GGGGCGTGAG
    AGACATCTCT TCTTGTTTGA AATGTCTTTG GTCTTCAGCA AAGAAGTGAA AGATTCAAGT
    GGAAGGAGCA AATATATTTA CAAATCAAAA TTATTTACCT CAGAGCTTGG AGTCACAGAA
    CATGTAGAAG GTGATCCTTG CAAATTTGCA CTTTGGGTAG GCAGAACGCC AACATCTGAT
    AACAAAATAG TCCTTAAGGG CTCAAGTATT GAGAACAAGC AAGATTGGAT CAAGCATATA
    AGAGAAGTGA TCCAAGACCG AACCATTCAC CTGAGAGGCG CATTAAAAGA GCCTATTCAT
    ATACCTAAAA CAGCTGCTGC TACAAAACAG AAACCTGCCA AAAGAGATGG AGAAGACCTG
    GACAGTCAAG GAGATGGCAG CAGCCAACCT GATACAATAT CTATTGCTTC ACGAACTTCA
    CAGAACACAT TAGACAGTGA TAAGCTTTCT GGTGGATGTG AGCTGACAGT AGTCATCCAT
    GACTTTACTG CCTGTAATAG CAATGAGCTG ACAATTAAAC GAGGGCAGAC TGTGGAGGTT
    CTAGAGCGGC ATCACGATCG ACCTGACTGG TGTTTAGTTC GGACTACTGA CCGGTCACCT
    GCAGCAGAGG GCTTGGTGCC TAGTAGTTCA CTGTGTATTG CACATTCTAG AAGTAGCATG
    GAAATGGAAG GCATATTTAA CCACAAAGAT TCCCTCTCTG TCTCCAGTAA TGATGCCAGT
    CCTCCTGCTT CAGTAATTTC CCTTCAGCCT CACATGATTG GTGCTCAAAG CTCTCCTGGC
    CCAAAGCGAC CTGGCAATAC TTTGAGAAAA TGGCTCACCA GCCCTGTCAG GAGACTTAGC
    AGTGGCAAAG CTGATGGACA TGTGAAAAAA TTGGCTCATA AACACAAAAA AAGCAGGGAG
    GTTCGTAAAA ACACAGACTC AGGTTCCCAA AAGGACTCTG ATGACAGTGC AGCAACACCT
    CAAGATGAAA CTATCGAGGA GAGAGTGCGC AATGAAGGAC TTAGCAGTGG TACGCTTTCT
    AAATCCTCCT CGTCTGGCAT GCAAAGCTGT GGAGAAGAAG AGGGCGAGGA AGGACCTGAT
    GCAGTTCCAT TGCCTCCACC CATGGCTATT CAGCAGCACA GCCTTCTTCA GCCTGACTCA
    CAGGATGACA AGACATCATC TCGGTTGCTG GTGCGACCTA CTAGCTCAGA GACACCTAGT
    GCTGCAGAGC TTGTTAGTGC TATTGAAGAA CTTGTAAAGA GTAAAATGGC TTTGGAAGAC
    CGGCCAAGTT CACTTCAAGT AGACCAGGGA GACAGTAGTA GCCCATCATT CAACCCTTCA
    GATACATCTC TGCTTTCCTC CTCTTCTCCA ATTGATGAAA TGGAAGAAAA AAAATCAAGC
    TCTTTAAAAA GAAGGCACTA TGTATTACAA GAGCTAGTAG AAACTGAACG CGATTATGTT
    CGGGACCTTG GCTATGTGGT TGAGGGTTAT ATGGCACTTA TGAAGGAGGA TGGAGTTCCA
    GATGATATGA AAGGAAAAGA CAAGATTGTT TTTGGCAACT TACACCAGAT CTACGAGTGG
    CACAGAGACT TTTTTTTGGG TGAACTGGAA AGGTGCCTGG ATGAGCCTGA AAGATTGGCA
    TCCCTATTTG TCAAGCATGA AAGAAGATTG CATATGTACA TAGTTTACTG CCAAAATAAA
    CCAAAGTCTG AACATATTGT TTCAGAATAC ATTGATACCT ATTTTGAGGA CCTGAAACAG
    CGTCTGGGCC ATCGACTTCA GCTTACAGAT CTGTTAATAA AACCCATACA AAGAATAATG
    AAATATCAGC TGCTATTAAA GGATTTTCTC AAGTACTCTA AGAAGGCTTG CATAGATGTA
    GCAGAACTTG AGAGAGCTGT GGAAGTCATG TGTATCGTAC CGAAGCGGTG TAATGACATG
    ATGAATGTTG GTCGTTTGCA AGGATTTGAT GGCAAAATAG TTGCCCAAGG AAAGCTGCTA
    CTGCAAGACA CATTCCTTGT GACTGAGCAG GACGCTGGTC TTCTGCCCCG ATGCAAAGAA
    AGGCGGGTCT TCCTTTTCGA ACAAATTGTC ATCTTTAGTG AGCCCTTAGA TAAAAAGAAA
    GGATACTCTA CACCTGGATT TCTTTTTAAA AATAATATCA AAGTGAGCTG CCTTAGTTTA
    GAGGAGTGTG TTGATAGTGA TCCATGCAAG TTTGCACTAT TGTCAAGGTC TGCTGACTCT
    ACAGAGATGT TTGTTTTGCA TTCTTCATCG CCTGGAGTGA GACAGTTATG GATTCATGAA
    ATCAACCAAA TTTTGGAAAA CCAGCGCAAT TTTTTAAATG CCTTGACATC ACCAATTGAA
    TACCACAGAA ATCACACTGG TGGAAGCAGT AGTGGCAACG TCACCTTTAC CAGCAGTCCC
    AGCAATTGTA GTGGGCTGGG TGGCTCCAGC CGAAGCAGGC CCTCTCGGAT TCCCCAGCCT
    GTTAGACACC ACTCTCCAGT CCTGGTCTCC TCTGCAGCTT CGGCACAGTC AGAGGCAGAC
    AAGATGTCAG GTATGTCAGC TCGCCGTGCA CCAGTTGTAC CTCTCAACCT CTCCTTAGAC
    AATAACCCCA GCCAGAAAGG TAGAGTAACT GCTGCTCCAG AGACAGGCGG GCGAGATAAA
    GAATCCAATT CAGTGCCAAA GATGAAAATG CTTGATGGCC CCAGGAAATC AGCTAGTGGC
    ATTCCAACCT TAGCTGATGG TGCACAGAAA GAATTGCGTG GGAGCACAGA AGACAGTCGA
    ATTAGAAGCA CTATGGGCAC TCTAACCTTA GGAAAGCCAA GAAATGGTTC TGCCTCACCC
    CTCAACTCTC CTGTCACAAT TAATTTTCCA TCGTCTTTTG GTAAGGAATC AGGGCCACCA
    AGCAGTCCCC TTCAGAAAGG CTCTTTTTGG AGCTCAATTC CAGGATCTCC AGCCAGCCGA
    CCTGGCTCCT TCACTTTCCC TGGAGACAGT GACTCTCTCC AGCGACAAGT TCATCGGCAC
    TCCACACACA GCAAGGATAC AGACCGCATG AGCACCTGCT CCTCGACTAG TGAGCAGTCC
    GTTCACTCCA CTCAGAGTAA TGGGGTAAGG ACAGAGAGTG AAAGTAGCAG CAGTAGCAAT
    ATCTCCACCA TGCTGGTTAC GCACGATTAT ACAGCGGTGA AGGAGGATGA GATCAATGTC
    TACCAGGGAG AGGTTGTACA AATTCTGGCA AGTAACCAAC AGAACATGTT TCTGGTGTTC
    AGAGCTGCCA CTGATGAGTG CCCTGCAGCT GAGGGCTGGA TTCCAAGCTA TGTTCTGGGT
    CACACAAGTG CAATCATCAC TGATATGACT GATGCAACTA TGAAGAAGTC CTCTTCTTGG
    CATACAGCCC TTAGAATAAG AAAGAAATCT GAGAAGAGGG AAAAAGATGG CAGCAGAGAC
    ACAAAACTAG AGAATGGTTA TCGCAAATCA AGAGAAGGAC TAGCTAATAA GCTCCTGAAT
    CCAAACTACA TTTATGATGC TCCTCCAGAG TTTATTGTAC CACTGAACGA GGTTACATGT
    GACACAGGAG AGACTGTGAT TATTCGATGC AAAGTTTGTG GTCGCCCCAA GGCATCCATT
    ACATGGAAAG GTCCCGACCA TAATACTTTG AGCAACGATG GACACTACAG TATATCCTAC
    AGTGATCTTG GAGAAGCAAC TCTAAAAATA GTTGGTGTGA CCACTGAGGA TGATGGTATA
    TACACCTGCA TTGCAACAAA TGACTTGGGT TCAGTATCCG CTTCCTCCAA TCTTAGAGTG
    TTAGGTTCAT GGAGTGATGG ACTGAGAGTA ATCTGGAAAG AAAACTTCGA CATTGCTTAT
    AGTGAAGTGG CTGAACTTGG CAGGGGCAGG TTTTCTGTCG TGAAAAAATG TGAACAAAGA
    GTTACAAGAC GCATAGTAGC CACCAAATTT GTTAACAAGA AGTTGATGAA AAGAGACCAG
    GTTACCCATG AACTTGGAAT TCTGCAGAGC CTTCAGCACC CACAACTGAT CAGCCTTCTT
    GACACTTTTG AGACACCAGC AAGCTATGTC TTAGTACTTG AGATAGCTGA TCAGGGATGT
    TTGTTAGAAT ATGTGGTACA GTGGGGAAAC CTTACAGAAG AAAAGATCAC CTCATATATG
    AGAGAGGTGC TGGAGGCCCT GCAGTATCTT CATAATTGCA GAATAGCACA TCTGGACCTA
    AAGCCTGAGA ACATTCTTGT GGATCAGAGC CTAACCAAAC CAACTATAAA ACTGTCAGAC
    TTTGGCGATG CTGTTCAGCT TAATACCACT TACTACATTC ACCAATTGTT GGGCAGTGCA
    GAGTTTGCCG CTCCAGAGAT CATCCTTGGG AATCCTGTCT CCCTTACCTC TGATATATGG
    AGTATTGGAG TTCTTACTTA TGTCCTTCTC AGTGGCGTCT CTCCTTTCCT TGATGAAAGT
    GTTGAAGAGA CATGCCTGAA CATTTGCCGA CTAGACTTTA GTTTTCCCGA TGACTACTTT
    AAGGGAGTAA GTCAGAAGGC CAAAGAATTT GTATGTTTCT TACTGCAGGG TGACCAGACA
    AAGCGCCCTT CTGCTGCTGT ATGTCTAAAG GAACAGTGGT TGCAGCCAGG CCAGCACAAA
    CGTAGTGGCA CATTAGACAT GTCAAGACTG ACCTCGTTTA TTGAACGCCG TAAACACCAG
    AATGATGTTC GACCCATTGG TAGCATTAAA AACTTCCTTC AGAGTCGCCT TTTACCCAGA
    ATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32256
    Clone ID Related Accession (Same CDS sequence) XM_012964356.2
    Accession Version XM_012964356.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8727bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product triple functional domain protein isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964356.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    121
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    541
    601
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    721
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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
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    1921
    1981
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    2641
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    4141
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    4681
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    6301
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    6421
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    7141
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    7261
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    7381
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    7681
    7741
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    7861
    7921
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    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    ATGGTGTCTT TAGAAGGCCT TACCAAAGTA GTTGACCCTT CCCAGCTAAC TCCAGAATTT 
    GAGGGTTGCT TGGAATACAA CCATGAAGAG TGGATAGAGA TCAGAGTAGC ATTTGAGGAT
    TTTATTAATA ATGCAACTCA TATGCTGTCT CGATTAGAGG AATTACAGGA GATGCTTGCA
    ATTAAAGAGT TGCCTCAGGA TTTAGAGGGG GCACGCAATA TGATTGAAGA ACATTCTCAA
    ATGAAAAAAA GGGTTATAAA GGCTCCTATT GAAGATCTTG ATTTAGAAGG GCAAAAGCTT
    TTACAAACAA TACAAAGCAG TGATAGCTTT CCTAAGAAGA ACTCTAGTTC TGGAAATGCA
    GACCTTCAAA ATCTTCTTCC CAAAGTTTCC ACAATGTTAG ACAGGCTTCA TTCAACACGA
    CAACATCTTC ATCAGATGTG GCACATGCGA AAAGTAAAAC TGGACCAGTG CTTTCAGCTA
    AGGTTGTTTG AGCAGGATGC TGAGAAGATG TTTGACTGGA TTACACACAA CAAAGGTCTG
    TTTCTGACAA GTTATACTGA CATTGGCACC CGTAATAATC ATGCTATGGA ACTTCAGACA
    CAGCACAATC ATTTTGCTAT GAACTGCATG AATGTCTATG TAAACATTAG CCGAATAATG
    TCTGTGGCAA ATCGACTAGT AGAATCTGGA CACTATGCCT CACAGCAAAT TAAGCAGATA
    GCTAACCAGT TGGAGCAAGA GTGGAAGGCT TTTGCCGCAG CCCTTGATGA ACGTAGTACG
    TTGCTTGACA TGTCTGCAAT GTTCCATCAA AAAGCGGAGC AGTACATGGG CAATGTGGAT
    TCATGGTGCA AAGCTTGTGG GGAAGTGGAT CTTCCATCAG AGCTGCAGGA TCTAGAAGAT
    GCCATTCATC ACCATCAGGG AATATATGAG CATATCACTT TAGCATATTC AGAGGTTAGC
    CAGGATGGTA AATCTTTATT AGACAAACTC CAGAGACCAT TGACTCCTGG TAGTTCTGAC
    TCACTGACAG CTTCTGCCAA CTATTCAAAG GCTGTGCATC ACGTCTTAGA TGCAATACAT
    GAAGTCTTGC ACCACCAGCG TCAGTTGGAA AACATCTGGC AACATCGCAA AGTTCGTCTG
    CATCAACGCC TACAGCTCTG TGTTTTTCAG CAAGATGTTC AGCAGGTCTT GGATTGGATA
    GAGAACCATG GTGAAGCCTT TCTCAGCAAA CACACTGGGG TAGGCAAGTC TCTGCACAGG
    GCCAGAGCCT TGCAAAAGCG ACATGAAGAC TTTGAAGAAG TCGCTCAGAA TACATACACC
    AATGCTGATA AGCTGCTGGA AGCTGCAGAA CAGTTGGCCC AGACAGGAGA GTGCGATCCA
    GAGGAAATAT ACCAGGCTGC TCATCAGCTT GAGGATCGGA TTCAGGATTT TGTAAGACGT
    GTAGAACAAA GAAAAGTTCT CTTGGACATG TCTGTATCAT TCCACTCGCA TGTTAAAGAG
    CTATGGACAT GGCTGGAGGA CCTACAAAAG GAACTGCTGG ATGATGTCTA TGCGGAGTCA
    GTAGAAGCTG TACAGGACTT GATCAAACGG TTTGGACAAC AGCAGCAGAC AACCCTCCAG
    TTTACAGTAA ATGTCATCAA AGAAGGGGAG GATCTAATTC AGCAACTAAG AGATTCTGCT
    ATATCCAGCA ACAAAACTCC TCACAACAGC TCAATCAATC ACATTGAGAC TGTCCTTCAA
    CAACTTGATG AGGCCCAATC ACAGATGGAG GAGCTTTTCC AAGAGAGAAA AATCAAACTT
    GAGCTATTCT TGCAGCTTCG CATCTTTGAG AGAGATGCCA TTGATATCAT CTCAGACTTG
    GAATCATGGA ATGATGAACT TTCTCAGCAA ATGAATGATT TTGACACAGA GGACCTCACA
    CTAGCCGAGC AAAGGCTACA GCATCACGCA GACAAAGCTC TGACAATGAA CAACCTGACA
    TTTGATGTCA TCCACCAAGG ACAAGAGTTA CTACAATATG TGAATGAAGT TCAGAACTCA
    GGGGTTGAGC TACTCTGTGA TAGAGATGTA GATATGGCTA CTAGAGTTCA GGACCTGTTG
    GAATTCCTCC ATGAGAAACA ACAGGAGTTG GATCTAGCAG CTGAGCAGCA CCGAAAACAT
    CTAGAACAAT GTGTGCAACT ACGACATTTG CAAGCTGAAG TCAAACAGGT ACTCGGCTGG
    ATACGGAATG GAGAGTCTAT GTTAAATGCC GGGCTTATCA CTGCCAGTTC ATTGCAAGAA
    GCAGAGCAAC TTCAAAGGGA ACATGAGCAG TTCCAGCATG CAATAGAAAA AACCCACCAG
    AGTGCTCTGC AGGTGCAGCA GAAGGCAGAG GCCATGCTTC AAGCAAACCA TTACGACATG
    GATATGATCC GGGACTGTGC TGAAAAAGTG GCCTCTCATT GGCAACAGTT GATGCTTAAA
    ATGGAAGACC GTCTTAAGCT TGTCAATGCC TCTGTGGCCT TTTATAAAAC CTCAGAGCAG
    GTATGCAGTG TTCTAGAGAG CCTAGAGCAG GAGTACAAGC GGGAGGAAGA TTGGTGTGGC
    GGAGCTGATA AACTTGGGCC TAACTCAGAG ACTGACCATG TTACTCCTAT GATCAGCAAA
    CACCTTGAAC AAAAGGAAGC CTTTCTTAAG GCCTGCACTT TGGCTCGCAG AAATGCTGAT
    GTCTTTCTGA AATACTTGCA CAGGAATAGT GTCAATATGC CTGGAATGGT GACACATGTA
    AAGGCTCCTG AACAGCAAGT AAAAAATATT TTAAATGAGC TGTTTCAGAG GGAAAATAGA
    GTTCTTCATT ATTGGACCTT GCGGAAAAGG AGACTAGACC ATTGCCAGCA GTATGTAGTA
    TTTGAAAGAA GTGCCAAACA GGCTCTGGAG TGGATCCAAG ATAATGGAGA ATTTTACATT
    TCCACACACA CCTCCACTGG CTCCACAATT CAGCATACAC AAGAACTCTT AAAAGAGCAT
    GAGGAGTTTC AGATCACAGC AAAGCAAACC AAAGAGAGGG TAAAGCTGTT GATACAGCTG
    GCTGATGGCT TTTGTGAAAA AGGGCATGCT CATGCTGCAG AAATTAAAAA ATGTGTTACA
    GCGGTGGACA AGAGGTACAG AGACTTCTCG CTGCGTATGG AAAAGTACAG AACCAGCTTA
    GAGAAGGCTT TGGGCATTTC TTCTGATTCC AAGTCGAGTA AAAGCCTTCA GTTGGATATT
    ATTCCAGCCA CTGCCCCTGG TTCCGAAGTC AAGCTTCGTG ATGCAACACA TGAACTCAAT
    GAGGAGAAAA GAAAATCTGC TCGTCGAAAA GAATTCATCA TGGCAGAACT TATACAAACT
    GAAAAAGCAT ATGTTCGTGA TCTTCGGGAA TGCATGGATA CATATCTGTG GGAAATGACA
    AGCGGAGTTG AAGAGATCCC ACCAGGCATT GTAAATAAAG AACACATTAT CTTTGGAAAC
    ATGCAGGAAA TATATGAATT TCATAACAAC ATATTCTTAA AGGAGCTTGA GAAGTATGAG
    CAGCTACCTG AAGATGTTGG ACATTGTTTT GTCACATGGG CTGACAAATT TCAGATGTAT
    GTGTCCTACT GTAAGAATAA GCCTGATTCA ACACAACTTA TCTTGGAACA AGCAGGAACA
    TATTTTGATG AAATTCAGCA GCGCCATGAG CTGGTCAACT CCATCTCTTC TTATCTTATC
    AAACCTGTGC AAAGGATAAC AAAATATCAG CTTCTTTTAA AAGAGCTGCT GACATGCTGC
    GAAGAAGGTA AAGGAGAGAT TAAAGATGGG CTGGAAGTGA TGCTAAGTGT ACCAAAAAGA
    GCCAACGATG CAATGCATCT CAGCATGCTT GAAGGGTTTG ATGAGAACAT TGAATCTCAG
    GGAGAACTTA TTCTGCAAGA GTCTTTTCAA GTGTGGGATC CTAAAACCTT AATAAGAAAG
    GGGCGTGAGA GACATCTCTT CTTGTTTGAA ATGTCTTTGG TCTTCAGCAA AGAAGTGAAA
    GATTCAAGTG GAAGGAGCAA ATATATTTAC AAATCAAAAT TATTTACCTC AGAGCTTGGA
    GTCACAGAAC ATGTAGAAGG TGATCCTTGC AAATTTGCAC TTTGGGTAGG CAGAACGCCA
    ACATCTGATA ACAAAATAGT CCTTAAGGGC TCAAGTATTG AGAACAAGCA AGATTGGATC
    AAGCATATAA GAGAAGTGAT CCAAGACCGA ACCATTCACC TGAGAGGCGC ATTAAAAGAG
    CCTATTCATA TACCTAAAAC AGCTGCTGCT ACAAAACAGA AACCTGCCAA AAGAGATGGA
    GAAGACCTGG ACAGTCAAGG AGATGGCAGC AGCCAACCTG ATACAATATC TATTGCTTCA
    CGAACTTCAC AGAACACATT AGACAGTGAT AAGCTTTCTG GTGGATGTGA GCTGACAGTA
    GTCATCCATG ACTTTACTGC CTGTAATAGC AATGAGCTGA CAATTAAACG AGGGCAGACT
    GTGGAGGTTC TAGAGCGGCA TCACGATCGA CCTGACTGGT GTTTAGTTCG GACTACTGAC
    CGGTCACCTG CAGCAGAGGG CTTGGTGCCT AGTAGTTCAC TGTGTATTGC ACATTCTAGA
    AGTAGCATGG AAATGGAAGG CATATTTAAC CACAAAGATT CCCTCTCTGT CTCCAGTAAT
    GATGCCAGTC CTCCTGCTTC AGTAATTTCC CTTCAGCCTC ACATGATTGG TGCTCAAAGC
    TCTCCTGGCC CAAAGCGACC TGGCAATACT TTGAGAAAAT GGCTCACCAG CCCTGTCAGG
    AGACTTAGCA GTGGCAAAGC TGATGGACAT GTGAAAAAAT TGGCTCATAA ACACAAAAAA
    AGCAGGGAGG TTCGTAAAAA CACAGACTCA GGTTCCCAAA AGGACTCTGA TGACAGTGCA
    GCAACACCTC AAGATGAAAC TATCGAGGAG AGAGTGCGCA ATGAAGGACT TAGCAGTGGT
    ACGCTTTCTA AATCCTCCTC GTCTGGCATG CAAAGCTGTG GAGAAGAAGA GGGCGAGGAA
    GGACCTGATG CAGTTCCATT GCCTCCACCC ATGGCTATTC AGCAGCACAG CCTTCTTCAG
    CCTGACTCAC AGGATGACAA GACATCATCT CGGTTGCTGG TGCGACCTAC TAGCTCAGAG
    ACACCTAGTG CTGCAGAGCT TGTTAGTGCT ATTGAAGAAC TTGTAAAGAG TAAAATGGCT
    TTGGAAGACC GGCCAAGTTC ACTTCAAGTA GACCAGGGAG ACAGTAGTAG CCCATCATTC
    AACCCTTCAG ATACATCTCT GCTTTCCTCC TCTTCTCCAA TTGATGAAAT GGAAGAAAAA
    AAATCAAGCT CTTTAAAAAG AAGGCACTAT GTATTACAAG AGCTAGTAGA AACTGAACGC
    GATTATGTTC GGGACCTTGG CTATGTGGTT GAGGGTTATA TGGCACTTAT GAAGGAGGAT
    GGAGTTCCAG ATGATATGAA AGGAAAAGAC AAGATTGTTT TTGGCAACTT ACACCAGATC
    TACGAGTGGC ACAGAGACTT TTTTTTGGGT GAACTGGAAA GGTGCCTGGA TGAGCCTGAA
    AGATTGGCAT CCCTATTTGT CAAGCATGAA AGAAGATTGC ATATGTACAT AGTTTACTGC
    CAAAATAAAC CAAAGTCTGA ACATATTGTT TCAGAATACA TTGATACCTA TTTTGAGGAC
    CTGAAACAGC GTCTGGGCCA TCGACTTCAG CTTACAGATC TGTTAATAAA ACCCATACAA
    AGAATAATGA AATATCAGCT GCTATTAAAG GATTTTCTCA AGTACTCTAA GAAGGCTTGC
    ATAGATGTAG CAGAACTTGA GAGAGCTGTG GAAGTCATGT GTATCGTACC GAAGCGGTGT
    AATGACATGA TGAATGTTGG TCGTTTGCAA GGATTTGATG GCAAAATAGT TGCCCAAGGA
    AAGCTGCTAC TGCAAGACAC ATTCCTTGTG ACTGAGCAGG ACGCTGGTCT TCTGCCCCGA
    TGCAAAGAAA GGCGGGTCTT CCTTTTCGAA CAAATTGTCA TCTTTAGTGA GCCCTTAGAT
    AAAAAGAAAG GATACTCTAC ACCTGGATTT CTTTTTAAAA ATAATATCAA AGTGAGCTGC
    CTTAGTTTAG AGGAGTGTGT TGATAGTGAT CCATGCAAGT TTGCACTATT GTCAAGGTCT
    GCTGACTCTA CAGAGATGTT TGTTTTGCAT TCTTCATCGC CTGGAGTGAG ACAGTTATGG
    ATTCATGAAA TCAACCAAAT TTTGGAAAAC CAGCGCAATT TTTTAAATGC CTTGACATCA
    CCAATTGAAT ACCACAGAAA TCACACTGGT GGAAGCAGTA GTGGCAACGT CACCTTTACC
    AGCAGTCCCA GCAATTGTAG TGGGCTGGGT GGCTCCAGCC GAAGCAGGCC CTCTCGGATT
    CCCCAGCCTG TTAGACACCA CTCTCCAGTC CTGGTCTCCT CTGCAGCTTC GGCACAGTCA
    GAGGCAGACA AGATGTCAGG TATGTCAGCT CGCCGTGCAC CAGTTGTACC TCTCAACCTC
    TCCTTAGACA ATAACCCCAG CCAGAAAGGT AGAGTAACTG CTGCTCCAGA GACAGGCGGG
    CGAGATAAAG AATCCAATTC AGTGCCAAAG ATGAAAATGC TTGATGGCCC CAGGAAATCA
    GCTAGTGGCA TTCCAACCTT AGCTGATGGT GCACAGAAAG AATTGCGTGG GAGCACAGAA
    GACAGTCGAA TTAGAAGCAC TATGGGCACT CTAACCTTAG GAAAGCCAAG AAATGGTTCT
    GCCTCACCCC TCAACTCTCC TGTCACAATT AATTTTCCAT CGTCTTTTGG TAAGGAATCA
    GGGCCACCAA GCAGTCCCCT TCAGAAAGGC TCTTTTTGGA GCTCAATTCC AGGATCTCCA
    GCCAGCCGAC CTGGCTCCTT CACTTTCCCT GGAGACAGTG ACTCTCTCCA GCGACAAGTT
    CATCGGCACT CCACACACAG CAAGGATACA GACCGCATGA GCACCTGCTC CTCGACTAGT
    GAGCAGTCCG TTCACTCCAC TCAGAGTAAT GGGGTAAGGA CAGAGAGTGA AAGTAGCAGC
    AGTAGCAATA TCTCCACCAT GCTGGTTACG CACGATTATA CAGCGGTGAA GGAGGATGAG
    ATCAATGTCT ACCAGGGAGA GGTTGTACAA ATTCTGGCAA GTAACCAACA GAACATGTTT
    CTGGTGTTCA GAGCTGCCAC TGATGAGTGC CCTGCAGCTG AGGGCTGGAT TCCAAGCTAT
    GTTCTGGGTC ACACAAGTGC AATCATCACT GATATGACTG ATGCAACTAT GAAGAAGTCC
    TCTTCTTGGC ATACAGCCCT TAGAATAAGA AAGAAATCTG AGAAGAGGGA AAAAGATGGC
    AGCAGAGACA CAAAACTAGA GAATGGTTAT CGCAAATCAA GAGAAGGACT AGCTAATAAG
    GTATCAGTTA AGCTCCTGAA TCCAAACTAC ATTTATGATG CTCCTCCAGA GTTTATTGTA
    CCACTGAACG AGGTTACATG TGACACAGGA GAGACTGTGA TTATTCGATG CAAAGTTTGT
    GGTCGCCCCA AGGCATCCAT TACATGGAAA GGTCCCGACC ATAATACTTT GAGCAACGAT
    GGACACTACA GTATATCCTA CAGTGATCTT GGAGAAGCAA CTCTAAAAAT AGTTGGTGTG
    ACCACTGAGG ATGATGGTAT ATACACCTGC ATTGCAACAA ATGACTTGGG TTCAGTATCC
    GCTTCCTCCA ATCTTAGAGT GTTAGGTTCA TGGAGTGATG GACTGAGAGT AATCTGGAAA
    GAAAACTTCG ACATTGCTTA TAGTGAAGTG GCTGAACTTG GCAGGGGCAG GTTTTCTGTC
    GTGAAAAAAT GTGAACAAAG AGTTACAAGA CGCATAGTAG CCACCAAATT TGTTAACAAG
    AAGTTGATGA AAAGAGACCA GGTTACCCAT GAACTTGGAA TTCTGCAGAG CCTTCAGCAC
    CCACAACTGA TCAGCCTTCT TGACACTTTT GAGACACCAG CAAGCTATGT CTTAGTACTT
    GAGATAGCTG ATCAGGGATG TTTGTTAGAA TATGTGGTAC AGTGGGGAAA CCTTACAGAA
    GAAAAGATCA CCTCATATAT GAGAGAGGTG CTGGAGGCCC TGCAGTATCT TCATAATTGC
    AGAATAGCAC ATCTGGACCT AAAGCCTGAG AACATTCTTG TGGATCAGAG CCTAACCAAA
    CCAACTATAA AACTGTCAGA CTTTGGCGAT GCTGTTCAGC TTAATACCAC TTACTACATT
    CACCAATTGT TGGGCAGTGC AGAGTTTGCC GCTCCAGAGA TCATCCTTGG GAATCCTGTC
    TCCCTTACCT CTGATATATG GAGTATTGGA GTTCTTACTT ATGTCCTTCT CAGTGGCGTC
    TCTCCTTTCC TTGATGAAAG TGTTGAAGAG ACATGCCTGA ACATTTGCCG ACTAGACTTT
    AGTTTTCCCG ATGACTACTT TAAGGGAGTA AGTCAGAAGG CCAAAGAATT TGTATGTTTC
    TTACTGCAGG GTGACCAGAC AAAGCGCCCT TCTGCTGCTG TATGTCTAAA GGAACAGTGG
    TTGCAGCCAG GCCAGCACAA ACGTAGTGGC ACATTAGACA TGTCAAGACT GACCTCGTTT
    ATTGAACGCC GTAAACACCA GAATGATGTT CGACCCATTG GTAGCATTAA AAACTTCCTT
    CAGAGTCGCC TTTTACCCAG AATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012819810.1
    CDS73..8799
    Translation

    Target ORF information:

    RefSeq Version XM_012964356.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012964356.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    ATGGTGTCTT TAGAAGGCCT TACCAAAGTA GTTGACCCTT CCCAGCTAAC TCCAGAATTT 
    GAGGGTTGCT TGGAATACAA CCATGAAGAG TGGATAGAGA TCAGAGTAGC ATTTGAGGAT
    TTTATTAATA ATGCAACTCA TATGCTGTCT CGATTAGAGG AATTACAGGA GATGCTTGCA
    ATTAAAGAGT TGCCTCAGGA TTTAGAGGGG GCACGCAATA TGATTGAAGA ACATTCTCAA
    ATGAAAAAAA GGGTTATAAA GGCTCCTATT GAAGATCTTG ATTTAGAAGG GCAAAAGCTT
    TTACAAACAA TACAAAGCAG TGATAGCTTT CCTAAGAAGA ACTCTAGTTC TGGAAATGCA
    GACCTTCAAA ATCTTCTTCC CAAAGTTTCC ACAATGTTAG ACAGGCTTCA TTCAACACGA
    CAACATCTTC ATCAGATGTG GCACATGCGA AAAGTAAAAC TGGACCAGTG CTTTCAGCTA
    AGGTTGTTTG AGCAGGATGC TGAGAAGATG TTTGACTGGA TTACACACAA CAAAGGTCTG
    TTTCTGACAA GTTATACTGA CATTGGCACC CGTAATAATC ATGCTATGGA ACTTCAGACA
    CAGCACAATC ATTTTGCTAT GAACTGCATG AATGTCTATG TAAACATTAG CCGAATAATG
    TCTGTGGCAA ATCGACTAGT AGAATCTGGA CACTATGCCT CACAGCAAAT TAAGCAGATA
    GCTAACCAGT TGGAGCAAGA GTGGAAGGCT TTTGCCGCAG CCCTTGATGA ACGTAGTACG
    TTGCTTGACA TGTCTGCAAT GTTCCATCAA AAAGCGGAGC AGTACATGGG CAATGTGGAT
    TCATGGTGCA AAGCTTGTGG GGAAGTGGAT CTTCCATCAG AGCTGCAGGA TCTAGAAGAT
    GCCATTCATC ACCATCAGGG AATATATGAG CATATCACTT TAGCATATTC AGAGGTTAGC
    CAGGATGGTA AATCTTTATT AGACAAACTC CAGAGACCAT TGACTCCTGG TAGTTCTGAC
    TCACTGACAG CTTCTGCCAA CTATTCAAAG GCTGTGCATC ACGTCTTAGA TGCAATACAT
    GAAGTCTTGC ACCACCAGCG TCAGTTGGAA AACATCTGGC AACATCGCAA AGTTCGTCTG
    CATCAACGCC TACAGCTCTG TGTTTTTCAG CAAGATGTTC AGCAGGTCTT GGATTGGATA
    GAGAACCATG GTGAAGCCTT TCTCAGCAAA CACACTGGGG TAGGCAAGTC TCTGCACAGG
    GCCAGAGCCT TGCAAAAGCG ACATGAAGAC TTTGAAGAAG TCGCTCAGAA TACATACACC
    AATGCTGATA AGCTGCTGGA AGCTGCAGAA CAGTTGGCCC AGACAGGAGA GTGCGATCCA
    GAGGAAATAT ACCAGGCTGC TCATCAGCTT GAGGATCGGA TTCAGGATTT TGTAAGACGT
    GTAGAACAAA GAAAAGTTCT CTTGGACATG TCTGTATCAT TCCACTCGCA TGTTAAAGAG
    CTATGGACAT GGCTGGAGGA CCTACAAAAG GAACTGCTGG ATGATGTCTA TGCGGAGTCA
    GTAGAAGCTG TACAGGACTT GATCAAACGG TTTGGACAAC AGCAGCAGAC AACCCTCCAG
    TTTACAGTAA ATGTCATCAA AGAAGGGGAG GATCTAATTC AGCAACTAAG AGATTCTGCT
    ATATCCAGCA ACAAAACTCC TCACAACAGC TCAATCAATC ACATTGAGAC TGTCCTTCAA
    CAACTTGATG AGGCCCAATC ACAGATGGAG GAGCTTTTCC AAGAGAGAAA AATCAAACTT
    GAGCTATTCT TGCAGCTTCG CATCTTTGAG AGAGATGCCA TTGATATCAT CTCAGACTTG
    GAATCATGGA ATGATGAACT TTCTCAGCAA ATGAATGATT TTGACACAGA GGACCTCACA
    CTAGCCGAGC AAAGGCTACA GCATCACGCA GACAAAGCTC TGACAATGAA CAACCTGACA
    TTTGATGTCA TCCACCAAGG ACAAGAGTTA CTACAATATG TGAATGAAGT TCAGAACTCA
    GGGGTTGAGC TACTCTGTGA TAGAGATGTA GATATGGCTA CTAGAGTTCA GGACCTGTTG
    GAATTCCTCC ATGAGAAACA ACAGGAGTTG GATCTAGCAG CTGAGCAGCA CCGAAAACAT
    CTAGAACAAT GTGTGCAACT ACGACATTTG CAAGCTGAAG TCAAACAGGT ACTCGGCTGG
    ATACGGAATG GAGAGTCTAT GTTAAATGCC GGGCTTATCA CTGCCAGTTC ATTGCAAGAA
    GCAGAGCAAC TTCAAAGGGA ACATGAGCAG TTCCAGCATG CAATAGAAAA AACCCACCAG
    AGTGCTCTGC AGGTGCAGCA GAAGGCAGAG GCCATGCTTC AAGCAAACCA TTACGACATG
    GATATGATCC GGGACTGTGC TGAAAAAGTG GCCTCTCATT GGCAACAGTT GATGCTTAAA
    ATGGAAGACC GTCTTAAGCT TGTCAATGCC TCTGTGGCCT TTTATAAAAC CTCAGAGCAG
    GTATGCAGTG TTCTAGAGAG CCTAGAGCAG GAGTACAAGC GGGAGGAAGA TTGGTGTGGC
    GGAGCTGATA AACTTGGGCC TAACTCAGAG ACTGACCATG TTACTCCTAT GATCAGCAAA
    CACCTTGAAC AAAAGGAAGC CTTTCTTAAG GCCTGCACTT TGGCTCGCAG AAATGCTGAT
    GTCTTTCTGA AATACTTGCA CAGGAATAGT GTCAATATGC CTGGAATGGT GACACATGTA
    AAGGCTCCTG AACAGCAAGT AAAAAATATT TTAAATGAGC TGTTTCAGAG GGAAAATAGA
    GTTCTTCATT ATTGGACCTT GCGGAAAAGG AGACTAGACC ATTGCCAGCA GTATGTAGTA
    TTTGAAAGAA GTGCCAAACA GGCTCTGGAG TGGATCCAAG ATAATGGAGA ATTTTACATT
    TCCACACACA CCTCCACTGG CTCCACAATT CAGCATACAC AAGAACTCTT AAAAGAGCAT
    GAGGAGTTTC AGATCACAGC AAAGCAAACC AAAGAGAGGG TAAAGCTGTT GATACAGCTG
    GCTGATGGCT TTTGTGAAAA AGGGCATGCT CATGCTGCAG AAATTAAAAA ATGTGTTACA
    GCGGTGGACA AGAGGTACAG AGACTTCTCG CTGCGTATGG AAAAGTACAG AACCAGCTTA
    GAGAAGGCTT TGGGCATTTC TTCTGATTCC AAGTCGAGTA AAAGCCTTCA GTTGGATATT
    ATTCCAGCCA CTGCCCCTGG TTCCGAAGTC AAGCTTCGTG ATGCAACACA TGAACTCAAT
    GAGGAGAAAA GAAAATCTGC TCGTCGAAAA GAATTCATCA TGGCAGAACT TATACAAACT
    GAAAAAGCAT ATGTTCGTGA TCTTCGGGAA TGCATGGATA CATATCTGTG GGAAATGACA
    AGCGGAGTTG AAGAGATCCC ACCAGGCATT GTAAATAAAG AACACATTAT CTTTGGAAAC
    ATGCAGGAAA TATATGAATT TCATAACAAC ATATTCTTAA AGGAGCTTGA GAAGTATGAG
    CAGCTACCTG AAGATGTTGG ACATTGTTTT GTCACATGGG CTGACAAATT TCAGATGTAT
    GTGTCCTACT GTAAGAATAA GCCTGATTCA ACACAACTTA TCTTGGAACA AGCAGGAACA
    TATTTTGATG AAATTCAGCA GCGCCATGAG CTGGTCAACT CCATCTCTTC TTATCTTATC
    AAACCTGTGC AAAGGATAAC AAAATATCAG CTTCTTTTAA AAGAGCTGCT GACATGCTGC
    GAAGAAGGTA AAGGAGAGAT TAAAGATGGG CTGGAAGTGA TGCTAAGTGT ACCAAAAAGA
    GCCAACGATG CAATGCATCT CAGCATGCTT GAAGGGTTTG ATGAGAACAT TGAATCTCAG
    GGAGAACTTA TTCTGCAAGA GTCTTTTCAA GTGTGGGATC CTAAAACCTT AATAAGAAAG
    GGGCGTGAGA GACATCTCTT CTTGTTTGAA ATGTCTTTGG TCTTCAGCAA AGAAGTGAAA
    GATTCAAGTG GAAGGAGCAA ATATATTTAC AAATCAAAAT TATTTACCTC AGAGCTTGGA
    GTCACAGAAC ATGTAGAAGG TGATCCTTGC AAATTTGCAC TTTGGGTAGG CAGAACGCCA
    ACATCTGATA ACAAAATAGT CCTTAAGGGC TCAAGTATTG AGAACAAGCA AGATTGGATC
    AAGCATATAA GAGAAGTGAT CCAAGACCGA ACCATTCACC TGAGAGGCGC ATTAAAAGAG
    CCTATTCATA TACCTAAAAC AGCTGCTGCT ACAAAACAGA AACCTGCCAA AAGAGATGGA
    GAAGACCTGG ACAGTCAAGG AGATGGCAGC AGCCAACCTG ATACAATATC TATTGCTTCA
    CGAACTTCAC AGAACACATT AGACAGTGAT AAGCTTTCTG GTGGATGTGA GCTGACAGTA
    GTCATCCATG ACTTTACTGC CTGTAATAGC AATGAGCTGA CAATTAAACG AGGGCAGACT
    GTGGAGGTTC TAGAGCGGCA TCACGATCGA CCTGACTGGT GTTTAGTTCG GACTACTGAC
    CGGTCACCTG CAGCAGAGGG CTTGGTGCCT AGTAGTTCAC TGTGTATTGC ACATTCTAGA
    AGTAGCATGG AAATGGAAGG CATATTTAAC CACAAAGATT CCCTCTCTGT CTCCAGTAAT
    GATGCCAGTC CTCCTGCTTC AGTAATTTCC CTTCAGCCTC ACATGATTGG TGCTCAAAGC
    TCTCCTGGCC CAAAGCGACC TGGCAATACT TTGAGAAAAT GGCTCACCAG CCCTGTCAGG
    AGACTTAGCA GTGGCAAAGC TGATGGACAT GTGAAAAAAT TGGCTCATAA ACACAAAAAA
    AGCAGGGAGG TTCGTAAAAA CACAGACTCA GGTTCCCAAA AGGACTCTGA TGACAGTGCA
    GCAACACCTC AAGATGAAAC TATCGAGGAG AGAGTGCGCA ATGAAGGACT TAGCAGTGGT
    ACGCTTTCTA AATCCTCCTC GTCTGGCATG CAAAGCTGTG GAGAAGAAGA GGGCGAGGAA
    GGACCTGATG CAGTTCCATT GCCTCCACCC ATGGCTATTC AGCAGCACAG CCTTCTTCAG
    CCTGACTCAC AGGATGACAA GACATCATCT CGGTTGCTGG TGCGACCTAC TAGCTCAGAG
    ACACCTAGTG CTGCAGAGCT TGTTAGTGCT ATTGAAGAAC TTGTAAAGAG TAAAATGGCT
    TTGGAAGACC GGCCAAGTTC ACTTCAAGTA GACCAGGGAG ACAGTAGTAG CCCATCATTC
    AACCCTTCAG ATACATCTCT GCTTTCCTCC TCTTCTCCAA TTGATGAAAT GGAAGAAAAA
    AAATCAAGCT CTTTAAAAAG AAGGCACTAT GTATTACAAG AGCTAGTAGA AACTGAACGC
    GATTATGTTC GGGACCTTGG CTATGTGGTT GAGGGTTATA TGGCACTTAT GAAGGAGGAT
    GGAGTTCCAG ATGATATGAA AGGAAAAGAC AAGATTGTTT TTGGCAACTT ACACCAGATC
    TACGAGTGGC ACAGAGACTT TTTTTTGGGT GAACTGGAAA GGTGCCTGGA TGAGCCTGAA
    AGATTGGCAT CCCTATTTGT CAAGCATGAA AGAAGATTGC ATATGTACAT AGTTTACTGC
    CAAAATAAAC CAAAGTCTGA ACATATTGTT TCAGAATACA TTGATACCTA TTTTGAGGAC
    CTGAAACAGC GTCTGGGCCA TCGACTTCAG CTTACAGATC TGTTAATAAA ACCCATACAA
    AGAATAATGA AATATCAGCT GCTATTAAAG GATTTTCTCA AGTACTCTAA GAAGGCTTGC
    ATAGATGTAG CAGAACTTGA GAGAGCTGTG GAAGTCATGT GTATCGTACC GAAGCGGTGT
    AATGACATGA TGAATGTTGG TCGTTTGCAA GGATTTGATG GCAAAATAGT TGCCCAAGGA
    AAGCTGCTAC TGCAAGACAC ATTCCTTGTG ACTGAGCAGG ACGCTGGTCT TCTGCCCCGA
    TGCAAAGAAA GGCGGGTCTT CCTTTTCGAA CAAATTGTCA TCTTTAGTGA GCCCTTAGAT
    AAAAAGAAAG GATACTCTAC ACCTGGATTT CTTTTTAAAA ATAATATCAA AGTGAGCTGC
    CTTAGTTTAG AGGAGTGTGT TGATAGTGAT CCATGCAAGT TTGCACTATT GTCAAGGTCT
    GCTGACTCTA CAGAGATGTT TGTTTTGCAT TCTTCATCGC CTGGAGTGAG ACAGTTATGG
    ATTCATGAAA TCAACCAAAT TTTGGAAAAC CAGCGCAATT TTTTAAATGC CTTGACATCA
    CCAATTGAAT ACCACAGAAA TCACACTGGT GGAAGCAGTA GTGGCAACGT CACCTTTACC
    AGCAGTCCCA GCAATTGTAG TGGGCTGGGT GGCTCCAGCC GAAGCAGGCC CTCTCGGATT
    CCCCAGCCTG TTAGACACCA CTCTCCAGTC CTGGTCTCCT CTGCAGCTTC GGCACAGTCA
    GAGGCAGACA AGATGTCAGG TATGTCAGCT CGCCGTGCAC CAGTTGTACC TCTCAACCTC
    TCCTTAGACA ATAACCCCAG CCAGAAAGGT AGAGTAACTG CTGCTCCAGA GACAGGCGGG
    CGAGATAAAG AATCCAATTC AGTGCCAAAG ATGAAAATGC TTGATGGCCC CAGGAAATCA
    GCTAGTGGCA TTCCAACCTT AGCTGATGGT GCACAGAAAG AATTGCGTGG GAGCACAGAA
    GACAGTCGAA TTAGAAGCAC TATGGGCACT CTAACCTTAG GAAAGCCAAG AAATGGTTCT
    GCCTCACCCC TCAACTCTCC TGTCACAATT AATTTTCCAT CGTCTTTTGG TAAGGAATCA
    GGGCCACCAA GCAGTCCCCT TCAGAAAGGC TCTTTTTGGA GCTCAATTCC AGGATCTCCA
    GCCAGCCGAC CTGGCTCCTT CACTTTCCCT GGAGACAGTG ACTCTCTCCA GCGACAAGTT
    CATCGGCACT CCACACACAG CAAGGATACA GACCGCATGA GCACCTGCTC CTCGACTAGT
    GAGCAGTCCG TTCACTCCAC TCAGAGTAAT GGGGTAAGGA CAGAGAGTGA AAGTAGCAGC
    AGTAGCAATA TCTCCACCAT GCTGGTTACG CACGATTATA CAGCGGTGAA GGAGGATGAG
    ATCAATGTCT ACCAGGGAGA GGTTGTACAA ATTCTGGCAA GTAACCAACA GAACATGTTT
    CTGGTGTTCA GAGCTGCCAC TGATGAGTGC CCTGCAGCTG AGGGCTGGAT TCCAAGCTAT
    GTTCTGGGTC ACACAAGTGC AATCATCACT GATATGACTG ATGCAACTAT GAAGAAGTCC
    TCTTCTTGGC ATACAGCCCT TAGAATAAGA AAGAAATCTG AGAAGAGGGA AAAAGATGGC
    AGCAGAGACA CAAAACTAGA GAATGGTTAT CGCAAATCAA GAGAAGGACT AGCTAATAAG
    GTATCAGTTA AGCTCCTGAA TCCAAACTAC ATTTATGATG CTCCTCCAGA GTTTATTGTA
    CCACTGAACG AGGTTACATG TGACACAGGA GAGACTGTGA TTATTCGATG CAAAGTTTGT
    GGTCGCCCCA AGGCATCCAT TACATGGAAA GGTCCCGACC ATAATACTTT GAGCAACGAT
    GGACACTACA GTATATCCTA CAGTGATCTT GGAGAAGCAA CTCTAAAAAT AGTTGGTGTG
    ACCACTGAGG ATGATGGTAT ATACACCTGC ATTGCAACAA ATGACTTGGG TTCAGTATCC
    GCTTCCTCCA ATCTTAGAGT GTTAGGTTCA TGGAGTGATG GACTGAGAGT AATCTGGAAA
    GAAAACTTCG ACATTGCTTA TAGTGAAGTG GCTGAACTTG GCAGGGGCAG GTTTTCTGTC
    GTGAAAAAAT GTGAACAAAG AGTTACAAGA CGCATAGTAG CCACCAAATT TGTTAACAAG
    AAGTTGATGA AAAGAGACCA GGTTACCCAT GAACTTGGAA TTCTGCAGAG CCTTCAGCAC
    CCACAACTGA TCAGCCTTCT TGACACTTTT GAGACACCAG CAAGCTATGT CTTAGTACTT
    GAGATAGCTG ATCAGGGATG TTTGTTAGAA TATGTGGTAC AGTGGGGAAA CCTTACAGAA
    GAAAAGATCA CCTCATATAT GAGAGAGGTG CTGGAGGCCC TGCAGTATCT TCATAATTGC
    AGAATAGCAC ATCTGGACCT AAAGCCTGAG AACATTCTTG TGGATCAGAG CCTAACCAAA
    CCAACTATAA AACTGTCAGA CTTTGGCGAT GCTGTTCAGC TTAATACCAC TTACTACATT
    CACCAATTGT TGGGCAGTGC AGAGTTTGCC GCTCCAGAGA TCATCCTTGG GAATCCTGTC
    TCCCTTACCT CTGATATATG GAGTATTGGA GTTCTTACTT ATGTCCTTCT CAGTGGCGTC
    TCTCCTTTCC TTGATGAAAG TGTTGAAGAG ACATGCCTGA ACATTTGCCG ACTAGACTTT
    AGTTTTCCCG ATGACTACTT TAAGGGAGTA AGTCAGAAGG CCAAAGAATT TGTATGTTTC
    TTACTGCAGG GTGACCAGAC AAAGCGCCCT TCTGCTGCTG TATGTCTAAA GGAACAGTGG
    TTGCAGCCAG GCCAGCACAA ACGTAGTGGC ACATTAGACA TGTCAAGACT GACCTCGTTT
    ATTGAACGCC GTAAACACCA GAATGATGTT CGACCCATTG GTAGCATTAA AAACTTCCTT
    CAGAGTCGCC TTTTACCCAG AATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa44862
    Clone ID Related Accession (Same CDS sequence) XM_018094560.1
    Accession Version XM_018094560.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8607bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product triple functional domain protein isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    ATGAGCACAT CCAGCACCGC AAGCTCCGGT AGCGCAAATA CCGGTACCGG CAATGCCGTT 
    GGAAGCAGCG GAGGCAACAC CGGGGAGGGG GCGGAGGAGA CGGCCAAGGA CTCTGCAGAC
    ATAGCTGCCT TTTACAGATC CGGTTTTCGT AGAAATGATG AATTGAAAGC AATGGAGGTG
    TTGCCAATTT TAAAAGAAAA AGTGGCATAT CTATCAGGTG GCAGAGATAA ACGAGGTGGT
    CCTGTATTAA CATTTCCAGC ACGCAGCAAC CATGATCGAA TACGACAAGA GGATCTCCGA
    AGACTGATTT CATATCTTGC TTGTATTCCT AGTGAGGAGG TGTGCAAGCG AGGCTTCACA
    GTGATTGTAG ATATGCGTGG TTCCAAGTGG GATTCTATTA AACCCTTGCT GAAAATTTTA
    CAAGAATCCT TTCCATGCTG TATTCATGTT GCACTAATTA TCAAACCAGA TAACTTCTGG
    CAGAAGCAAA GAACTAATTT TGGAAGTTCT AAGTTTGAAT TTGAGACAAG TATGGTGTCT
    TTAGAAGGCC TTACCAAAGT AGTTGACCCT TCCCAGCTAA CTCCAGAATT TGAGGGTTGC
    TTGGAATACA ACCATGAAGA GTGGATAGAG ATCAGAGTAG CATTTGAGGA TTTTATTAAT
    AATGCAACTC ATATGCTGTC TCGATTAGAG GAATTACAGG AGATGCTTGC AATTAAAGAG
    TTGCCTCAGG ATTTAGAGGG GGCACGCAAT ATGATTGAAG AACATTCTCA AATGAAAAAA
    AGGGTTATAA AGGCTCCTAT TGAAGATCTT GATTTAGAAG GGCAAAAGCT TTTACAAACA
    ATACAAAGCA GTGATAGCTT TCCTAAGAAG AACTCTAGTT CTGGAAATGC AGACCTTCAA
    AATCTTCTTC CCAAAGTTTC CACAATGTTA GACAGGCTTC ATTCAACACG ACAACATCTT
    CATCAGATGT GGCACATGCG AAAAGTAAAA CTGGACCAGT GCTTTCAGCT AAGGTTGTTT
    GAGCAGGATG CTGAGAAGAT GTTTGACTGG ATTACACACA ACAAAGGTCT GTTTCTGACA
    AGTTATACTG ACATTGGCAC CCGTAATAAT CATGCTATGG AACTTCAGAC ACAGCACAAT
    CATTTTGCTA TGAACTGCAT GAATGTCTAT GTAAACATTA GCCGAATAAT GTCTGTGGCA
    AATCGACTAG TAGAATCTGG ACACTATGCC TCACAGCAAA TTAAGCAGAT AGCTAACCAG
    TTGGAGCAAG AGTGGAAGGC TTTTGCCGCA GCCCTTGATG AACGTAGTAC GTTGCTTGAC
    ATGTCTGCAA TGTTCCATCA AAAAGCGGAG CAGTACATGG GCAATGTGGA TTCATGGTGC
    AAAGCTTGTG GGGAAGTGGA TCTTCCATCA GAGCTGCAGG ATCTAGAAGA TGCCATTCAT
    CACCATCAGG GAATATATGA GCATATCACT TTAGCATATT CAGAGGTTAG CCAGGATGGT
    AAATCTTTAT TAGACAAACT CCAGAGACCA TTGACTCCTG GTAGTTCTGA CTCACTGACA
    GCTTCTGCCA ACTATTCAAA GGCTGTGCAT CACGTCTTAG ATGCAATACA TGAAGTCTTG
    CACCACCAGC GTCAGTTGGA AAACATCTGG CAACATCGCA AAGTTCGTCT GCATCAACGC
    CTACAGCTCT GTGTTTTTCA GCAAGATGTT CAGCAGGTCT TGGATTGGAT AGAGAACCAT
    GGTGAAGCCT TTCTCAGCAA ACACACTGGG GTAGGCAAGT CTCTGCACAG GGCCAGAGCC
    TTGCAAAAGC GACATGAAGA CTTTGAAGAA GTCGCTCAGA ATACATACAC CAATGCTGAT
    AAGCTGCTGG AAGCTGCAGA ACAGTTGGCC CAGACAGGAG AGTGCGATCC AGAGGAAATA
    TACCAGGCTG CTCATCAGCT TGAGGATCGG ATTCAGGATT TTGTAAGACG TGTAGAACAA
    AGAAAAGTTC TCTTGGACAT GTCTGTATCA TTCCACTCGC ATGTTAAAGA GCTATGGACA
    TGGCTGGAGG ACCTACAAAA GGAACTGCTG GATGATGTCT ATGCGGAGTC AGTAGAAGCT
    GTACAGGACT TGATCAAACG GTTTGGACAA CAGCAGCAGA CAACCCTCCA GTTTACAGTA
    AATGTCATCA AAGAAGGGGA GGATCTAATT CAGCAACTAA GAGATTCTGC TATATCCAGC
    AACAAAACTC CTCACAACAG CTCAATCAAT CACATTGAGA CTGTCCTTCA ACAACTTGAT
    GAGGCCCAAT CACAGATGGA GGAGCTTTTC CAAGAGAGAA AAATCAAACT TGAGCTATTC
    TTGCAGCTTC GCATCTTTGA GAGAGATGCC ATTGATATCA TCTCAGACTT GGAATCATGG
    AATGATGAAC TTTCTCAGCA AATGAATGAT TTTGACACAG AGGACCTCAC ACTAGCCGAG
    CAAAGGCTAC AGCATCACGC AGACAAAGCT CTGACAATGA ACAACCTGAC ATTTGATGTC
    ATCCACCAAG GACAAGAGTT ACTACAATAT GTGAATGAAG TTCAGAACTC AGGGGTTGAG
    CTACTCTGTG ATAGAGATGT AGATATGGCT ACTAGAGTTC AGGACCTGTT GGAATTCCTC
    CATGAGAAAC AACAGGAGTT GGATCTAGCA GCTGAGCAGC ACCGAAAACA TCTAGAACAA
    TGTGTGCAAC TACGACATTT GCAAGCTGAA GTCAAACAGG TACTCGGCTG GATACGGAAT
    GGAGAGTCTA TGTTAAATGC CGGGCTTATC ACTGCCAGTT CATTGCAAGA AGCAGAGCAA
    CTTCAAAGGG AACATGAGCA GTTCCAGCAT GCAATAGAAA AAACCCACCA GAGTGCTCTG
    CAGGTGCAGC AGAAGGCAGA GGCCATGCTT CAAGCAAACC ATTACGACAT GGATATGATC
    CGGGACTGTG CTGAAAAAGT GGCCTCTCAT TGGCAACAGT TGATGCTTAA AATGGAAGAC
    CGTCTTAAGC TTGTCAATGC CTCTGTGGCC TTTTATAAAA CCTCAGAGCA GGTATGCAGT
    GTTCTAGAGA GCCTAGAGCA GGAGTACAAG CGGGAGGAAG ATTGGTGTGG CGGAGCTGAT
    AAACTTGGGC CTAACTCAGA GACTGACCAT GTTACTCCTA TGATCAGCAA ACACCTTGAA
    CAAAAGGAAG CCTTTCTTAA GGCCTGCACT TTGGCTCGCA GAAATGCTGA TGTCTTTCTG
    AAATACTTGC ACAGGAATAG TGTCAATATG CCTGGAATGG TGACACATGT AAAGGCTCCT
    GAACAGCAAG TAAAAAATAT TTTAAATGAG CTGTTTCAGA GGGAAAATAG AGTTCTTCAT
    TATTGGACCT TGCGGAAAAG GAGACTAGAC CATTGCCAGC AGTATGTAGT ATTTGAAAGA
    AGTGCCAAAC AGGCTCTGGA GTGGATCCAA GATAATGGAG AATTTTACAT TTCCACACAC
    ACCTCCACTG GCTCCACAAT TCAGCATACA CAAGAACTCT TAAAAGAGCA TGAGGAGTTT
    CAGATCACAG CAAAGCAAAC CAAAGAGAGG GTAAAGCTGT TGATACAGCT GGCTGATGGC
    TTTTGTGAAA AAGGGCATGC TCATGCTGCA GAAATTAAAA AATGTGTTAC AGCGGTGGAC
    AAGAGGTACA GAGACTTCTC GCTGCGTATG GAAAAGTACA GAACCAGCTT AGAGAAGGCT
    TTGGGCATTT CTTCTGATTC CAAGTCGAGT AAAAGCCTTC AGTTGGATAT TATTCCAGCC
    ACTGCCCCTG GTTCCGAAGT CAAGCTTCGT GATGCAACAC ATGAACTCAA TGAGGAGAAA
    AGAAAATCTG CTCGTCGAAA AGAATTCATC ATGGCAGAAC TTATACAAAC TGAAAAAGCA
    TATGTTCGTG ATCTTCGGGA ATGCATGGAT ACATATCTGT GGGAAATGAC AAGCGGAGTT
    GAAGAGATCC CACCAGGCAT TGTAAATAAA GAACACATTA TCTTTGGAAA CATGCAGGAA
    ATATATGAAT TTCATAACAA CATATTCTTA AAGGAGCTTG AGAAGTATGA GCAGCTACCT
    GAAGATGTTG GACATTGTTT TGTCACATGG GCTGACAAAT TTCAGATGTA TGTGTCCTAC
    TGTAAGAATA AGCCTGATTC AACACAACTT ATCTTGGAAC AAGCAGGAAC ATATTTTGAT
    GAAATTCAGC AGCGCCATGA GCTGGTCAAC TCCATCTCTT CTTATCTTAT CAAACCTGTG
    CAAAGGATAA CAAAATATCA GCTTCTTTTA AAAGAGCTGC TGACATGCTG CGAAGAAGGT
    AAAGGAGAGA TTAAAGATGG GCTGGAAGTG ATGCTAAGTG TACCAAAAAG AGCCAACGAT
    GCAATGCATC TCAGCATGCT TGAAGGGTTT GATGAGAACA TTGAATCTCA GGGAGAACTT
    ATTCTGCAAG AGTCTTTTCA AGTGTGGGAT CCTAAAACCT TAATAAGAAA GGGGCGTGAG
    AGACATCTCT TCTTGTTTGA AATGTCTTTG GTCTTCAGCA AAGAAGTGAA AGATTCAAGT
    GGAAGGAGCA AATATATTTA CAAATCAAAA TTATTTACCT CAGAGCTTGG AGTCACAGAA
    CATGTAGAAG GTGATCCTTG CAAATTTGCA CTTTGGGTAG GCAGAACGCC AACATCTGAT
    AACAAAATAG TCCTTAAGGG CTCAAGTATT GAGAACAAGC AAGATTGGAT CAAGCATATA
    AGAGAAGTGA TCCAAGACCG AACCATTCAC CTGAGAGGCG CATTAAAAGA GCCTATTCAT
    ATACCTAAAA CAGCTGCTGC TACAAAACAG AAACCTGCCA AAAGAGATGG AGAAGACCTG
    GACAGTCAAG GAGATGGCAG CAGCCAACCT GATACAATAT CTATTGCTTC ACGAACTTCA
    CAGAACACAT TAGACAGTGA TAAGCTTTCT GGTGGATGTG AGCTGACAGT AGTCATCCAT
    GACTTTACTG CCTGTAATAG CAATGAGCTG ACAATTAAAC GAGGGCAGAC TGTGGAGGTT
    CTAGAGCGGC ATCACGATCG ACCTGACTGG TGTTTAGTTC GGACTACTGA CCGGTCACCT
    GCAGCAGAGG GCTTGGTGCC TAGTAGTTCA CTGTGTATTG CACATTCTAG AAGTAGCATG
    GAAATGGAAG GCATATTTAA CCACAAAGAT TCCCTCTCTG TCTCCAGTAA TGATGCCAGT
    CCTCCTGCTT CAGTAATTTC CCTTCAGCCT CACATGATTG GTGCTCAAAG CTCTCCTGGC
    CCAAAGCGAC CTGGCAATAC TTTGAGAAAA TGGCTCACCA GCCCTGTCAG GAGACTTAGC
    AGTGGCAAAG CTGATGGACA TGTGAAAAAA TTGGCTCATA AACACAAAAA AAGCAGGGAG
    GTTCGTAAAA ACACAGACTC AGGTTCCCAA AAGGACTCTG ATGACAGTGC AGCAACACCT
    CAAGATGAAA CTATCGAGGA GAGAGTGCGC AATGAAGGAC TTAGCAGTGG TACGCTTTCT
    AAATCCTCCT CGTCTGGCAT GCAAAGCTGT GGAGAAGAAG AGGGCGAGGA AGGACCTGAT
    GCAGTTCCAT TGCCTCCACC CATGGCTATT CAGCAGCACA GCCTTCTTCA GCCTGACTCA
    CAGGATGACA AGACATCATC TCGGTTGCTG GTGCGACCTA CTAGCTCAGA GACACCTAGT
    GCTGCAGAGC TTGTTAGTGC TATTGAAGAA CTTGTAAAGA GTAAAATGGC TTTGGAAGAC
    CGGCCAAGTT CACTTCAAGT AGACCAGGGA GACAGTAGTA GCCCATCATT CAACCCTTCA
    GATACATCTC TGCTTTCCTC CTCTTCTCCA ATTGATGAAA TGGAAGAAAA AAAATCAAGC
    TCTTTAAAAA GAAGGCACTA TGTATTACAA GAGCTAGTAG AAACTGAACG CGATTATGTT
    CGGGACCTTG GCTATGTGGT TGAGGGTTAT ATGGCACTTA TGAAGGAGGA TGGAGTTCCA
    GATGATATGA AAGGAAAAGA CAAGATTGTT TTTGGCAACT TACACCAGAT CTACGAGTGG
    CACAGAGACT TTTTTTTGGG TGAACTGGAA AGGTGCCTGG ATGAGCCTGA AAGATTGGCA
    TCCCTATTTG TCAAGCATGA AAGAAGATTG CATATGTACA TAGTTTACTG CCAAAATAAA
    CCAAAGTCTG AACATATTGT TTCAGAATAC ATTGATACCT ATTTTGAGGA CCTGAAACAG
    CGTCTGGGCC ATCGACTTCA GCTTACAGAT CTGTTAATAA AACCCATACA AAGAATAATG
    AAATATCAGC TGCTATTAAA GGATTTTCTC AAGTACTCTA AGAAGGCTTG CATAGATGTA
    GCAGAACTTG AGAGAGCTGT GGAAGTCATG TGTATCGTAC CGAAGCGGTG TAATGACATG
    ATGAATGTTG GTCGTTTGCA AGGATTTGAT GGCAAAATAG TTGCCCAAGG AAAGCTGCTA
    CTGCAAGACA CATTCCTTGT GACTGAGCAG GACGCTGGTC TTCTGCCCCG ATGCAAAGAA
    AGGCGGGTCT TCCTTTTCGA ACAAATTGTC ATCTTTAGTG AGCCCTTAGA TAAAAAGAAA
    GGATACTCTA CACCTGGATT TCTTTTTAAA AATAATATCA AAGTGAGCTG CCTTAGTTTA
    GAGGAGTGTG TTGATAGTGA TCCATGCAAG TTTGCACTAT TGTCAAGGTC TGCTGACTCT
    ACAGAGATGT TTGTTTTGCA TTCTTCATCG CCTGGAGTGA GACAGTTATG GATTCATGAA
    ATCAACCAAA TTTTGGAAAA CCAGCGCAAT TTTTTAAATG CCTTGACATC ACCAATTGAA
    TACCACAGAA ATCACACTGG TGGAAGCAGT AGTGGCAACG TCACCTTTAC CAGCAGTCCC
    AGCAATTGTA GTGGGCTGGG TGGCTCCAGC CGAAGCAGGC CCTCTCGGAT TCCCCAGCCT
    GTTAGACACC ACTCTCCAGT CCTGGTCTCC TCTGCAGCTT CGGCACAGTC AGAGGCAGAC
    AAGATGTCAG GTATGTCAGC TCGCCGTGCA CCAGTTGTAC CTCTCAACCT CTCCTTAGAC
    AATAACCCCA GCCAGAAAGG TAGAGTAACT GCTGCTCCAG AGACAGGCGG GCGAGATAAA
    GAATCCAATT CAGTGCCAAA GATGAAAATG CTTGATGGCC CCAGGAAATC AGCTAGTGGC
    ATTCCAACCT TAGCTGATGG TGCACAGAAA GAATTGCGTG GGAGCACAGA AGACAGTCGA
    ATTAGAAGCA CTATGGGCAC TCTAACCTTA GGAAAGCCAA GAAATGGTTC TGCCTCACCC
    CTCAACTCTC CTGTCACAAT TAATTTTCCA TCGTCTTTTG GTAAGGAATC AGGGCCACCA
    AGCAGTCCCC TTCAGAAAGG CTCTTTTTGG AGCTCAATTC CAGGATCTCC AGCCAGCCGA
    CCTGGCTCCT TCACTTTCCC TGGAGACAGT GACTCTCTCC AGCGACAAGT TCATCGGCAC
    TCCACACACA GCAAGGATAC AGACCGCATG AGCACCTGCT CCTCGACTAG TGAGCAGTCC
    GTTCACTCCA CTCAGAGTAA TGGGGTAAGG ACAGAGAGTG AAAGTAGCAG CAGTAGCAAT
    ATCTCCACCA TGCTGGTTAC GCACGATTAT ACAGCGGTGA AGGAGGATGA GATCAATGTC
    TACCAGGGAG AGGTTGTACA AATTCTGGCA AGTAACCAAC AGAACATGTT TCTGGTGTTC
    AGAGCTGCCA CTGATGAGTG CCCTGCAGCT GAGGGCTGGA TTCCAAGCTA TGTTCTGGGT
    CACACAAGTG CAATCATCAC TGATATGACT GATGCAACTA TGAAGAAGTC CTCTTCTTGG
    CATACAGCCC TTAGAATAAG AAAGAAATCT GAGAAGAGGG AAAAAGATGG CAGCAGAGAC
    ACAAAACTAG AGAATGGTTA TCGCAAATCA AGAGAAGGAC TAGCTAATAA GGTATCAGTT
    AAGCTCCTGA ATCCAAACTA CATTTATGAT GCTCCTCCAG AGTTTATTGT ACCACTGAAC
    GAGGTTACAT GTGACACAGG AGAGACTGTG ATTATTCGAT GCAAAGTTTG TGGTCGCCCC
    AAGGCATCCA TTACATGGAA AGGTCCCGAC CATAATACTT TGAGCAACGA TGGACACTAC
    AGTATATCCT ACAGTGATCT TGGAGAAGCA ACTCTAAAAA TAGTTGGTGT GACCACTGAG
    GATGATGGTA TATACACCTG CATTGCAACA AATGACTTGG GTTCAGTATC CGCTTCCTCC
    AATCTTAGAG TGTTAGGTTC ATGGAGTGAT GGACTGAGAG TAATCTGGAA AGAAAACTTC
    GACATTGCTT ATAGTGAAGT GGCTGAACTT GGCAGGGGCA GGTTTTCTGT CGTGAAAAAA
    TGTGAACAAA GAGTTACAAG ACGCATAGTA GCCACCAAAT TTGTTAACAA GAAGTTGATG
    AAAAGAGACC AGGTTACCCA TGAACTTGGA ATTCTGCAGA GCCTTCAGCA CCCACAACTG
    ATCAGCCTTC TTGACACTTT TGAGACACCA GCAAGCTATG TCTTAGTACT TGAGATCCTG
    AGAACATTCT TGTGGATCAG AGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017950049.1
    CDS619..9225
    Translation

    Target ORF information:

    RefSeq Version XM_018094560.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018094560.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    ATGAGCACAT CCAGCACCGC AAGCTCCGGT AGCGCAAATA CCGGTACCGG CAATGCCGTT 
    GGAAGCAGCG GAGGCAACAC CGGGGAGGGG GCGGAGGAGA CGGCCAAGGA CTCTGCAGAC
    ATAGCTGCCT TTTACAGATC CGGTTTTCGT AGAAATGATG AATTGAAAGC AATGGAGGTG
    TTGCCAATTT TAAAAGAAAA AGTGGCATAT CTATCAGGTG GCAGAGATAA ACGAGGTGGT
    CCTGTATTAA CATTTCCAGC ACGCAGCAAC CATGATCGAA TACGACAAGA GGATCTCCGA
    AGACTGATTT CATATCTTGC TTGTATTCCT AGTGAGGAGG TGTGCAAGCG AGGCTTCACA
    GTGATTGTAG ATATGCGTGG TTCCAAGTGG GATTCTATTA AACCCTTGCT GAAAATTTTA
    CAAGAATCCT TTCCATGCTG TATTCATGTT GCACTAATTA TCAAACCAGA TAACTTCTGG
    CAGAAGCAAA GAACTAATTT TGGAAGTTCT AAGTTTGAAT TTGAGACAAG TATGGTGTCT
    TTAGAAGGCC TTACCAAAGT AGTTGACCCT TCCCAGCTAA CTCCAGAATT TGAGGGTTGC
    TTGGAATACA ACCATGAAGA GTGGATAGAG ATCAGAGTAG CATTTGAGGA TTTTATTAAT
    AATGCAACTC ATATGCTGTC TCGATTAGAG GAATTACAGG AGATGCTTGC AATTAAAGAG
    TTGCCTCAGG ATTTAGAGGG GGCACGCAAT ATGATTGAAG AACATTCTCA AATGAAAAAA
    AGGGTTATAA AGGCTCCTAT TGAAGATCTT GATTTAGAAG GGCAAAAGCT TTTACAAACA
    ATACAAAGCA GTGATAGCTT TCCTAAGAAG AACTCTAGTT CTGGAAATGC AGACCTTCAA
    AATCTTCTTC CCAAAGTTTC CACAATGTTA GACAGGCTTC ATTCAACACG ACAACATCTT
    CATCAGATGT GGCACATGCG AAAAGTAAAA CTGGACCAGT GCTTTCAGCT AAGGTTGTTT
    GAGCAGGATG CTGAGAAGAT GTTTGACTGG ATTACACACA ACAAAGGTCT GTTTCTGACA
    AGTTATACTG ACATTGGCAC CCGTAATAAT CATGCTATGG AACTTCAGAC ACAGCACAAT
    CATTTTGCTA TGAACTGCAT GAATGTCTAT GTAAACATTA GCCGAATAAT GTCTGTGGCA
    AATCGACTAG TAGAATCTGG ACACTATGCC TCACAGCAAA TTAAGCAGAT AGCTAACCAG
    TTGGAGCAAG AGTGGAAGGC TTTTGCCGCA GCCCTTGATG AACGTAGTAC GTTGCTTGAC
    ATGTCTGCAA TGTTCCATCA AAAAGCGGAG CAGTACATGG GCAATGTGGA TTCATGGTGC
    AAAGCTTGTG GGGAAGTGGA TCTTCCATCA GAGCTGCAGG ATCTAGAAGA TGCCATTCAT
    CACCATCAGG GAATATATGA GCATATCACT TTAGCATATT CAGAGGTTAG CCAGGATGGT
    AAATCTTTAT TAGACAAACT CCAGAGACCA TTGACTCCTG GTAGTTCTGA CTCACTGACA
    GCTTCTGCCA ACTATTCAAA GGCTGTGCAT CACGTCTTAG ATGCAATACA TGAAGTCTTG
    CACCACCAGC GTCAGTTGGA AAACATCTGG CAACATCGCA AAGTTCGTCT GCATCAACGC
    CTACAGCTCT GTGTTTTTCA GCAAGATGTT CAGCAGGTCT TGGATTGGAT AGAGAACCAT
    GGTGAAGCCT TTCTCAGCAA ACACACTGGG GTAGGCAAGT CTCTGCACAG GGCCAGAGCC
    TTGCAAAAGC GACATGAAGA CTTTGAAGAA GTCGCTCAGA ATACATACAC CAATGCTGAT
    AAGCTGCTGG AAGCTGCAGA ACAGTTGGCC CAGACAGGAG AGTGCGATCC AGAGGAAATA
    TACCAGGCTG CTCATCAGCT TGAGGATCGG ATTCAGGATT TTGTAAGACG TGTAGAACAA
    AGAAAAGTTC TCTTGGACAT GTCTGTATCA TTCCACTCGC ATGTTAAAGA GCTATGGACA
    TGGCTGGAGG ACCTACAAAA GGAACTGCTG GATGATGTCT ATGCGGAGTC AGTAGAAGCT
    GTACAGGACT TGATCAAACG GTTTGGACAA CAGCAGCAGA CAACCCTCCA GTTTACAGTA
    AATGTCATCA AAGAAGGGGA GGATCTAATT CAGCAACTAA GAGATTCTGC TATATCCAGC
    AACAAAACTC CTCACAACAG CTCAATCAAT CACATTGAGA CTGTCCTTCA ACAACTTGAT
    GAGGCCCAAT CACAGATGGA GGAGCTTTTC CAAGAGAGAA AAATCAAACT TGAGCTATTC
    TTGCAGCTTC GCATCTTTGA GAGAGATGCC ATTGATATCA TCTCAGACTT GGAATCATGG
    AATGATGAAC TTTCTCAGCA AATGAATGAT TTTGACACAG AGGACCTCAC ACTAGCCGAG
    CAAAGGCTAC AGCATCACGC AGACAAAGCT CTGACAATGA ACAACCTGAC ATTTGATGTC
    ATCCACCAAG GACAAGAGTT ACTACAATAT GTGAATGAAG TTCAGAACTC AGGGGTTGAG
    CTACTCTGTG ATAGAGATGT AGATATGGCT ACTAGAGTTC AGGACCTGTT GGAATTCCTC
    CATGAGAAAC AACAGGAGTT GGATCTAGCA GCTGAGCAGC ACCGAAAACA TCTAGAACAA
    TGTGTGCAAC TACGACATTT GCAAGCTGAA GTCAAACAGG TACTCGGCTG GATACGGAAT
    GGAGAGTCTA TGTTAAATGC CGGGCTTATC ACTGCCAGTT CATTGCAAGA AGCAGAGCAA
    CTTCAAAGGG AACATGAGCA GTTCCAGCAT GCAATAGAAA AAACCCACCA GAGTGCTCTG
    CAGGTGCAGC AGAAGGCAGA GGCCATGCTT CAAGCAAACC ATTACGACAT GGATATGATC
    CGGGACTGTG CTGAAAAAGT GGCCTCTCAT TGGCAACAGT TGATGCTTAA AATGGAAGAC
    CGTCTTAAGC TTGTCAATGC CTCTGTGGCC TTTTATAAAA CCTCAGAGCA GGTATGCAGT
    GTTCTAGAGA GCCTAGAGCA GGAGTACAAG CGGGAGGAAG ATTGGTGTGG CGGAGCTGAT
    AAACTTGGGC CTAACTCAGA GACTGACCAT GTTACTCCTA TGATCAGCAA ACACCTTGAA
    CAAAAGGAAG CCTTTCTTAA GGCCTGCACT TTGGCTCGCA GAAATGCTGA TGTCTTTCTG
    AAATACTTGC ACAGGAATAG TGTCAATATG CCTGGAATGG TGACACATGT AAAGGCTCCT
    GAACAGCAAG TAAAAAATAT TTTAAATGAG CTGTTTCAGA GGGAAAATAG AGTTCTTCAT
    TATTGGACCT TGCGGAAAAG GAGACTAGAC CATTGCCAGC AGTATGTAGT ATTTGAAAGA
    AGTGCCAAAC AGGCTCTGGA GTGGATCCAA GATAATGGAG AATTTTACAT TTCCACACAC
    ACCTCCACTG GCTCCACAAT TCAGCATACA CAAGAACTCT TAAAAGAGCA TGAGGAGTTT
    CAGATCACAG CAAAGCAAAC CAAAGAGAGG GTAAAGCTGT TGATACAGCT GGCTGATGGC
    TTTTGTGAAA AAGGGCATGC TCATGCTGCA GAAATTAAAA AATGTGTTAC AGCGGTGGAC
    AAGAGGTACA GAGACTTCTC GCTGCGTATG GAAAAGTACA GAACCAGCTT AGAGAAGGCT
    TTGGGCATTT CTTCTGATTC CAAGTCGAGT AAAAGCCTTC AGTTGGATAT TATTCCAGCC
    ACTGCCCCTG GTTCCGAAGT CAAGCTTCGT GATGCAACAC ATGAACTCAA TGAGGAGAAA
    AGAAAATCTG CTCGTCGAAA AGAATTCATC ATGGCAGAAC TTATACAAAC TGAAAAAGCA
    TATGTTCGTG ATCTTCGGGA ATGCATGGAT ACATATCTGT GGGAAATGAC AAGCGGAGTT
    GAAGAGATCC CACCAGGCAT TGTAAATAAA GAACACATTA TCTTTGGAAA CATGCAGGAA
    ATATATGAAT TTCATAACAA CATATTCTTA AAGGAGCTTG AGAAGTATGA GCAGCTACCT
    GAAGATGTTG GACATTGTTT TGTCACATGG GCTGACAAAT TTCAGATGTA TGTGTCCTAC
    TGTAAGAATA AGCCTGATTC AACACAACTT ATCTTGGAAC AAGCAGGAAC ATATTTTGAT
    GAAATTCAGC AGCGCCATGA GCTGGTCAAC TCCATCTCTT CTTATCTTAT CAAACCTGTG
    CAAAGGATAA CAAAATATCA GCTTCTTTTA AAAGAGCTGC TGACATGCTG CGAAGAAGGT
    AAAGGAGAGA TTAAAGATGG GCTGGAAGTG ATGCTAAGTG TACCAAAAAG AGCCAACGAT
    GCAATGCATC TCAGCATGCT TGAAGGGTTT GATGAGAACA TTGAATCTCA GGGAGAACTT
    ATTCTGCAAG AGTCTTTTCA AGTGTGGGAT CCTAAAACCT TAATAAGAAA GGGGCGTGAG
    AGACATCTCT TCTTGTTTGA AATGTCTTTG GTCTTCAGCA AAGAAGTGAA AGATTCAAGT
    GGAAGGAGCA AATATATTTA CAAATCAAAA TTATTTACCT CAGAGCTTGG AGTCACAGAA
    CATGTAGAAG GTGATCCTTG CAAATTTGCA CTTTGGGTAG GCAGAACGCC AACATCTGAT
    AACAAAATAG TCCTTAAGGG CTCAAGTATT GAGAACAAGC AAGATTGGAT CAAGCATATA
    AGAGAAGTGA TCCAAGACCG AACCATTCAC CTGAGAGGCG CATTAAAAGA GCCTATTCAT
    ATACCTAAAA CAGCTGCTGC TACAAAACAG AAACCTGCCA AAAGAGATGG AGAAGACCTG
    GACAGTCAAG GAGATGGCAG CAGCCAACCT GATACAATAT CTATTGCTTC ACGAACTTCA
    CAGAACACAT TAGACAGTGA TAAGCTTTCT GGTGGATGTG AGCTGACAGT AGTCATCCAT
    GACTTTACTG CCTGTAATAG CAATGAGCTG ACAATTAAAC GAGGGCAGAC TGTGGAGGTT
    CTAGAGCGGC ATCACGATCG ACCTGACTGG TGTTTAGTTC GGACTACTGA CCGGTCACCT
    GCAGCAGAGG GCTTGGTGCC TAGTAGTTCA CTGTGTATTG CACATTCTAG AAGTAGCATG
    GAAATGGAAG GCATATTTAA CCACAAAGAT TCCCTCTCTG TCTCCAGTAA TGATGCCAGT
    CCTCCTGCTT CAGTAATTTC CCTTCAGCCT CACATGATTG GTGCTCAAAG CTCTCCTGGC
    CCAAAGCGAC CTGGCAATAC TTTGAGAAAA TGGCTCACCA GCCCTGTCAG GAGACTTAGC
    AGTGGCAAAG CTGATGGACA TGTGAAAAAA TTGGCTCATA AACACAAAAA AAGCAGGGAG
    GTTCGTAAAA ACACAGACTC AGGTTCCCAA AAGGACTCTG ATGACAGTGC AGCAACACCT
    CAAGATGAAA CTATCGAGGA GAGAGTGCGC AATGAAGGAC TTAGCAGTGG TACGCTTTCT
    AAATCCTCCT CGTCTGGCAT GCAAAGCTGT GGAGAAGAAG AGGGCGAGGA AGGACCTGAT
    GCAGTTCCAT TGCCTCCACC CATGGCTATT CAGCAGCACA GCCTTCTTCA GCCTGACTCA
    CAGGATGACA AGACATCATC TCGGTTGCTG GTGCGACCTA CTAGCTCAGA GACACCTAGT
    GCTGCAGAGC TTGTTAGTGC TATTGAAGAA CTTGTAAAGA GTAAAATGGC TTTGGAAGAC
    CGGCCAAGTT CACTTCAAGT AGACCAGGGA GACAGTAGTA GCCCATCATT CAACCCTTCA
    GATACATCTC TGCTTTCCTC CTCTTCTCCA ATTGATGAAA TGGAAGAAAA AAAATCAAGC
    TCTTTAAAAA GAAGGCACTA TGTATTACAA GAGCTAGTAG AAACTGAACG CGATTATGTT
    CGGGACCTTG GCTATGTGGT TGAGGGTTAT ATGGCACTTA TGAAGGAGGA TGGAGTTCCA
    GATGATATGA AAGGAAAAGA CAAGATTGTT TTTGGCAACT TACACCAGAT CTACGAGTGG
    CACAGAGACT TTTTTTTGGG TGAACTGGAA AGGTGCCTGG ATGAGCCTGA AAGATTGGCA
    TCCCTATTTG TCAAGCATGA AAGAAGATTG CATATGTACA TAGTTTACTG CCAAAATAAA
    CCAAAGTCTG AACATATTGT TTCAGAATAC ATTGATACCT ATTTTGAGGA CCTGAAACAG
    CGTCTGGGCC ATCGACTTCA GCTTACAGAT CTGTTAATAA AACCCATACA AAGAATAATG
    AAATATCAGC TGCTATTAAA GGATTTTCTC AAGTACTCTA AGAAGGCTTG CATAGATGTA
    GCAGAACTTG AGAGAGCTGT GGAAGTCATG TGTATCGTAC CGAAGCGGTG TAATGACATG
    ATGAATGTTG GTCGTTTGCA AGGATTTGAT GGCAAAATAG TTGCCCAAGG AAAGCTGCTA
    CTGCAAGACA CATTCCTTGT GACTGAGCAG GACGCTGGTC TTCTGCCCCG ATGCAAAGAA
    AGGCGGGTCT TCCTTTTCGA ACAAATTGTC ATCTTTAGTG AGCCCTTAGA TAAAAAGAAA
    GGATACTCTA CACCTGGATT TCTTTTTAAA AATAATATCA AAGTGAGCTG CCTTAGTTTA
    GAGGAGTGTG TTGATAGTGA TCCATGCAAG TTTGCACTAT TGTCAAGGTC TGCTGACTCT
    ACAGAGATGT TTGTTTTGCA TTCTTCATCG CCTGGAGTGA GACAGTTATG GATTCATGAA
    ATCAACCAAA TTTTGGAAAA CCAGCGCAAT TTTTTAAATG CCTTGACATC ACCAATTGAA
    TACCACAGAA ATCACACTGG TGGAAGCAGT AGTGGCAACG TCACCTTTAC CAGCAGTCCC
    AGCAATTGTA GTGGGCTGGG TGGCTCCAGC CGAAGCAGGC CCTCTCGGAT TCCCCAGCCT
    GTTAGACACC ACTCTCCAGT CCTGGTCTCC TCTGCAGCTT CGGCACAGTC AGAGGCAGAC
    AAGATGTCAG GTATGTCAGC TCGCCGTGCA CCAGTTGTAC CTCTCAACCT CTCCTTAGAC
    AATAACCCCA GCCAGAAAGG TAGAGTAACT GCTGCTCCAG AGACAGGCGG GCGAGATAAA
    GAATCCAATT CAGTGCCAAA GATGAAAATG CTTGATGGCC CCAGGAAATC AGCTAGTGGC
    ATTCCAACCT TAGCTGATGG TGCACAGAAA GAATTGCGTG GGAGCACAGA AGACAGTCGA
    ATTAGAAGCA CTATGGGCAC TCTAACCTTA GGAAAGCCAA GAAATGGTTC TGCCTCACCC
    CTCAACTCTC CTGTCACAAT TAATTTTCCA TCGTCTTTTG GTAAGGAATC AGGGCCACCA
    AGCAGTCCCC TTCAGAAAGG CTCTTTTTGG AGCTCAATTC CAGGATCTCC AGCCAGCCGA
    CCTGGCTCCT TCACTTTCCC TGGAGACAGT GACTCTCTCC AGCGACAAGT TCATCGGCAC
    TCCACACACA GCAAGGATAC AGACCGCATG AGCACCTGCT CCTCGACTAG TGAGCAGTCC
    GTTCACTCCA CTCAGAGTAA TGGGGTAAGG ACAGAGAGTG AAAGTAGCAG CAGTAGCAAT
    ATCTCCACCA TGCTGGTTAC GCACGATTAT ACAGCGGTGA AGGAGGATGA GATCAATGTC
    TACCAGGGAG AGGTTGTACA AATTCTGGCA AGTAACCAAC AGAACATGTT TCTGGTGTTC
    AGAGCTGCCA CTGATGAGTG CCCTGCAGCT GAGGGCTGGA TTCCAAGCTA TGTTCTGGGT
    CACACAAGTG CAATCATCAC TGATATGACT GATGCAACTA TGAAGAAGTC CTCTTCTTGG
    CATACAGCCC TTAGAATAAG AAAGAAATCT GAGAAGAGGG AAAAAGATGG CAGCAGAGAC
    ACAAAACTAG AGAATGGTTA TCGCAAATCA AGAGAAGGAC TAGCTAATAA GGTATCAGTT
    AAGCTCCTGA ATCCAAACTA CATTTATGAT GCTCCTCCAG AGTTTATTGT ACCACTGAAC
    GAGGTTACAT GTGACACAGG AGAGACTGTG ATTATTCGAT GCAAAGTTTG TGGTCGCCCC
    AAGGCATCCA TTACATGGAA AGGTCCCGAC CATAATACTT TGAGCAACGA TGGACACTAC
    AGTATATCCT ACAGTGATCT TGGAGAAGCA ACTCTAAAAA TAGTTGGTGT GACCACTGAG
    GATGATGGTA TATACACCTG CATTGCAACA AATGACTTGG GTTCAGTATC CGCTTCCTCC
    AATCTTAGAG TGTTAGGTTC ATGGAGTGAT GGACTGAGAG TAATCTGGAA AGAAAACTTC
    GACATTGCTT ATAGTGAAGT GGCTGAACTT GGCAGGGGCA GGTTTTCTGT CGTGAAAAAA
    TGTGAACAAA GAGTTACAAG ACGCATAGTA GCCACCAAAT TTGTTAACAA GAAGTTGATG
    AAAAGAGACC AGGTTACCCA TGAACTTGGA ATTCTGCAGA GCCTTCAGCA CCCACAACTG
    ATCAGCCTTC TTGACACTTT TGAGACACCA GCAAGCTATG TCTTAGTACT TGAGATCCTG
    AGAACATTCT TGTGGATCAG AGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa59535
    Clone ID Related Accession (Same CDS sequence) XM_031903241.1
    Accession Version XM_031903241.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9159bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product triple functional domain protein isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    ATGCAGACTC ATCGCTGGAA AGATGACGGA GAAACAACAT TAGGTTTTCG TAGAAATGAT 
    GAATTGAAAG CAATGGAGGT GTTGCCAATT TTAAAAGAAA AAGTGGCATA TCTATCAGGT
    GGCAGAGATA AACGAGGTGG TCCTGTATTA ACATTTCCAG CACGCAGCAA CCATGATCGA
    ATACGACAAG AGGATCTCCG AAGACTGATT TCATATCTTG CTTGTATTCC TAGTGAGGAG
    GTGTGCAAGC GAGGCTTCAC AGTGATTGTA GATATGCGTG GTTCCAAGTG GGATTCTATT
    AAACCCTTGC TGAAAATTTT ACAAGAATCC TTTCCATGCT GTATTCATGT TGCACTAATT
    ATCAAACCAG ATAACTTCTG GCAGAAGCAA AGAACTAATT TTGGAAGTTC TAAGTTTGAA
    TTTGAGACAA GTATGGTGTC TTTAGAAGGC CTTACCAAAG TAGTTGACCC TTCCCAGCTA
    ACTCCAGAAT TTGAGGGTTG CTTGGAATAC AACCATGAAG AGTGGATAGA GATCAGAGTA
    GCATTTGAGG ATTTTATTAA TAATGCAACT CATATGCTGT CTCGATTAGA GGAATTACAG
    GAGATGCTTG CAATTAAAGA GTTGCCTCAG GATTTAGAGG GGGCACGCAA TATGATTGAA
    GAACATTCTC AAATGAAAAA AAGGGTTATA AAGGCTCCTA TTGAAGATCT TGATTTAGAA
    GGGCAAAAGC TTTTACAAAC AATACAAAGC AGTGATAGCT TTCCTAAGAA GAACTCTAGT
    TCTGGAAATG CAGACCTTCA AAATCTTCTT CCCAAAGTTT CCACAATGTT AGACAGGCTT
    CATTCAACAC GACAACATCT TCATCAGATG TGGCACATGC GAAAAGTAAA ACTGGACCAG
    TGCTTTCAGC TAAGGTTGTT TGAGCAGGAT GCTGAGAAGA TGTTTGACTG GATTACACAC
    AACAAAGGTC TGTTTCTGAC AAGTTATACT GACATTGGCA CCCGTAATAA TCATGCTATG
    GAACTTCAGA CACAGCACAA TCATTTTGCT ATGAACTGCA TGAATGTCTA TGTAAACATT
    AGCCGAATAA TGTCTGTGGC AAATCGACTA GTAGAATCTG GACACTATGC CTCACAGCAA
    ATTAAGCAGA TAGCTAACCA GTTGGAGCAA GAGTGGAAGG CTTTTGCCGC AGCCCTTGAT
    GAACGTAGTA CGTTGCTTGA CATGTCTGCA ATGTTCCATC AAAAAGCGGA GCAGTACATG
    GGCAATGTGG ATTCATGGTG CAAAGCTTGT GGGGAAGTGG ATCTTCCATC AGAGCTGCAG
    GATCTAGAAG ATGCCATTCA TCACCATCAG GGAATATATG AGCATATCAC TTTAGCATAT
    TCAGAGGTTA GCCAGGATGG TAAATCTTTA TTAGACAAAC TCCAGAGACC ATTGACTCCT
    GGTAGTTCTG ACTCACTGAC AGCTTCTGCC AACTATTCAA AGGCTGTGCA TCACGTCTTA
    GATGCAATAC ATGAAGTCTT GCACCACCAG CGTCAGTTGG AAAACATCTG GCAACATCGC
    AAAGTTCGTC TGCATCAACG CCTACAGCTC TGTGTTTTTC AGCAAGATGT TCAGCAGGTC
    TTGGATTGGA TAGAGAACCA TGGTGAAGCC TTTCTCAGCA AACACACTGG GGTAGGCAAG
    TCTCTGCACA GGGCCAGAGC CTTGCAAAAG CGACATGAAG ACTTTGAAGA AGTCGCTCAG
    AATACATACA CCAATGCTGA TAAGCTGCTG GAAGCTGCAG AACAGTTGGC CCAGACAGGA
    GAGTGCGATC CAGAGGAAAT ATACCAGGCT GCTCATCAGC TTGAGGATCG GATTCAGGAT
    TTTGTAAGAC GTGTAGAACA AAGAAAAGTT CTCTTGGACA TGTCTGTATC ATTCCACTCG
    CATGTTAAAG AGCTATGGAC ATGGCTGGAG GACCTACAAA AGGAACTGCT GGATGATGTC
    TATGCGGAGT CAGTAGAAGC TGTACAGGAC TTGATCAAAC GGTTTGGACA ACAGCAGCAG
    ACAACCCTCC AGTTTACAGT AAATGTCATC AAAGAAGGGG AGGATCTAAT TCAGCAACTA
    AGAGATTCTG CTATATCCAG CAACAAAACT CCTCACAACA GCTCAATCAA TCACATTGAG
    ACTGTCCTTC AACAACTTGA TGAGGCCCAA TCACAGATGG AGGAGCTTTT CCAAGAGAGA
    AAAATCAAAC TTGAGCTATT CTTGCAGCTT CGCATCTTTG AGAGAGATGC CATTGATATC
    ATCTCAGACT TGGAATCATG GAATGATGAA CTTTCTCAGC AAATGAATGA TTTTGACACA
    GAGGACCTCA CACTAGCCGA GCAAAGGCTA CAGCATCACG CAGACAAAGC TCTGACAATG
    AACAACCTGA CATTTGATGT CATCCACCAA GGACAAGAGT TACTACAATA TGTGAATGAA
    GTTCAGAACT CAGGGGTTGA GCTACTCTGT GATAGAGATG TAGATATGGC TACTAGAGTT
    CAGGACCTGT TGGAATTCCT CCATGAGAAA CAACAGGAGT TGGATCTAGC AGCTGAGCAG
    CACCGAAAAC ATCTAGAACA ATGTGTGCAA CTACGACATT TGCAAGCTGA AGTCAAACAG
    GTACTCGGCT GGATACGGAA TGGAGAGTCT ATGTTAAATG CCGGGCTTAT CACTGCCAGT
    TCATTGCAAG AAGCAGAGCA ACTTCAAAGG GAACATGAGC AGTTCCAGCA TGCAATAGAA
    AAAACCCACC AGAGTGCTCT GCAGGTGCAG CAGAAGGCAG AGGCCATGCT TCAAGCAAAC
    CATTACGACA TGGATATGAT CCGGGACTGT GCTGAAAAAG TGGCCTCTCA TTGGCAACAG
    TTGATGCTTA AAATGGAAGA CCGTCTTAAG CTTGTCAATG CCTCTGTGGC CTTTTATAAA
    ACCTCAGAGC AGGTATGCAG TGTTCTAGAG AGCCTAGAGC AGGAGTACAA GCGGGAGGAA
    GATTGGTGTG GCGGAGCTGA TAAACTTGGG CCTAACTCAG AGACTGACCA TGTTACTCCT
    ATGATCAGCA AACACCTTGA ACAAAAGGAA GCCTTTCTTA AGGCCTGCAC TTTGGCTCGC
    AGAAATGCTG ATGTCTTTCT GAAATACTTG CACAGGAATA GTGTCAATAT GCCTGGAATG
    GTGACACATG TAAAGGCTCC TGAACAGCAA GTAAAAAATA TTTTAAATGA GCTGTTTCAG
    AGGGAAAATA GAGTTCTTCA TTATTGGACC TTGCGGAAAA GGAGACTAGA CCATTGCCAG
    CAGTATGTAG TATTTGAAAG AAGTGCCAAA CAGGCTCTGG AGTGGATCCA AGATAATGGA
    GAATTTTACA TTTCCACACA CACCTCCACT GGCTCCACAA TTCAGCATAC ACAAGAACTC
    TTAAAAGAGC ATGAGGAGTT TCAGATCACA GCAAAGCAAA CCAAAGAGAG GGTAAAGCTG
    TTGATACAGC TGGCTGATGG CTTTTGTGAA AAAGGGCATG CTCATGCTGC AGAAATTAAA
    AAATGTGTTA CAGCGGTGGA CAAGAGGTAC AGAGACTTCT CGCTGCGTAT GGAAAAGTAC
    AGAACCAGCT TAGAGAAGGC TTTGGGCATT TCTTCTGATT CCAAGTCGAG TAAAAGCCTT
    CAGTTGGATA TTATTCCAGC CACTGCCCCT GGTTCCGAAG TCAAGCTTCG TGATGCAACA
    CATGAACTCA ATGAGGAGAA AAGAAAATCT GCTCGTCGAA AAGAATTCAT CATGGCAGAA
    CTTATACAAA CTGAAAAAGC ATATGTTCGT GATCTTCGGG AATGCATGGA TACATATCTG
    TGGGAAATGA CAAGCGGAGT TGAAGAGATC CCACCAGGCA TTGTAAATAA AGAACACATT
    ATCTTTGGAA ACATGCAGGA AATATATGAA TTTCATAACA ACATATTCTT AAAGGAGCTT
    GAGAAGTATG AGCAGCTACC TGAAGATGTT GGACATTGTT TTGTCACATG GGCTGACAAA
    TTTCAGATGT ATGTGTCCTA CTGTAAGAAT AAGCCTGATT CAACACAACT TATCTTGGAA
    CAAGCAGGAA CATATTTTGA TGAAATTCAG CAGCGCCATG AGCTGGTCAA CTCCATCTCT
    TCTTATCTTA TCAAACCTGT GCAAAGGATA ACAAAATATC AGCTTCTTTT AAAAGAGCTG
    CTGACATGCT GCGAAGAAGG TAAAGGAGAG ATTAAAGATG GGCTGGAAGT GATGCTAAGT
    GTACCAAAAA GAGCCAACGA TGCAATGCAT CTCAGCATGC TTGAAGGGTT TGATGAGAAC
    ATTGAATCTC AGGGAGAACT TATTCTGCAA GAGTCTTTTC AAGTGTGGGA TCCTAAAACC
    TTAATAAGAA AGGGGCGTGA GAGACATCTC TTCTTGTTTG AAATGTCTTT GGTCTTCAGC
    AAAGAAGTGA AAGATTCAAG TGGAAGGAGC AAATATATTT ACAAATCAAA ATTATTTACC
    TCAGAGCTTG GAGTCACAGA ACATGTAGAA GGTGATCCTT GCAAATTTGC ACTTTGGGTA
    GGCAGAACGC CAACATCTGA TAACAAAATA GTCCTTAAGG GCTCAAGTAT TGAGAACAAG
    CAAGATTGGA TCAAGCATAT AAGAGAAGTG ATCCAAGACC GAACCATTCA CCTGAGAGGC
    GCATTAAAAG AGCCTATTCA TATACCTAAA ACAGCTGCTG CTACAAAACA GAAACCTGCC
    AAAAGAGATG GAGAAGACCT GGACAGTCAA GGAGATGGCA GCAGCCAACC TGATACAATA
    TCTATTGCTT CACGAACTTC ACAGAACACA TTAGACAGTG ATAAGCTTTC TGGTGGATGT
    GAGCTGACAG TAGTCATCCA TGACTTTACT GCCTGTAATA GCAATGAGCT GACAATTAAA
    CGAGGGCAGA CTGTGGAGGT TCTAGAGCGG CATCACGATC GACCTGACTG GTGTTTAGTT
    CGGACTACTG ACCGGTCACC TGCAGCAGAG GGCTTGGTGC CTAGTAGTTC ACTGTGTATT
    GCACATTCTA GAAGTAGCAT GGAAATGGAA GGCATATTTA ACCACAAAGA TTCCCTCTCT
    GTCTCCAGTA ATGATGCCAG TCCTCCTGCT TCAGTAATTT CCCTTCAGCC TCACATGATT
    GGTGCTCAAA GCTCTCCTGG CCCAAAGCGA CCTGGCAATA CTTTGAGAAA ATGGCTCACC
    AGCCCTGTCA GGAGACTTAG CAGTGGCAAA GCTGATGGAC ATGTGAAAAA ATTGGCTCAT
    AAACACAAAA AAAGCAGGGA GGTTCGTAAA AACACAGACT CAGGTTCCCA AAAGGACTCT
    GATGACAGTG CAGCAACACC TCAAGATGAA ACTATCGAGG AGAGAGTGCG CAATGAAGGA
    CTTAGCAGTG GTACGCTTTC TAAATCCTCC TCGTCTGGCA TGCAAAGCTG TGGAGAAGAA
    GAGGGCGAGG AAGGACCTGA TGCAGTTCCA TTGCCTCCAC CCATGGCTAT TCAGCAGCAC
    AGCCTTCTTC AGCCTGACTC ACAGGATGAC AAGACATCAT CTCGGTTGCT GGTGCGACCT
    ACTAGCTCAG AGACACCTAG TGCTGCAGAG CTTGTTAGTG CTATTGAAGA ACTTGTAAAG
    AGTAAAATGG CTTTGGAAGA CCGGCCAAGT TCACTTCAAG TAGACCAGGG AGACAGTAGT
    AGCCCATCAT TCAACCCTTC AGATACATCT CTGCTTTCCT CCTCTTCTCC AATTGATGAA
    ATGGAAGAAA AAAAATCAAG CTCTTTAAAA AGAAGGCACT ATGTATTACA AGAGCTAGTA
    GAAACTGAAC GCGATTATGT TCGGGACCTT GGCTATGTGG TTGAGGGTTA TATGGCACTT
    ATGAAGGAGG ATGGAGTTCC AGATGATATG AAAGGAAAAG ACAAGATTGT TTTTGGCAAC
    TTACACCAGA TCTACGAGTG GCACAGAGAC TTTTTTTTGG GTGAACTGGA AAGGTGCCTG
    GATGAGCCTG AAAGATTGGC ATCCCTATTT GTCAAGCATG AAAGAAGATT GCATATGTAC
    ATAGTTTACT GCCAAAATAA ACCAAAGTCT GAACATATTG TTTCAGAATA CATTGATACC
    TATTTTGAGG ACCTGAAACA GCGTCTGGGC CATCGACTTC AGCTTACAGA TCTGTTAATA
    AAACCCATAC AAAGAATAAT GAAATATCAG CTGCTATTAA AGGATTTTCT CAAGTACTCT
    AAGAAGGCTT GCATAGATGT AGCAGAACTT GAGAGAGCTG TGGAAGTCAT GTGTATCGTA
    CCGAAGCGGT GTAATGACAT GATGAATGTT GGTCGTTTGC AAGGATTTGA TGGCAAAATA
    GTTGCCCAAG GAAAGCTGCT ACTGCAAGAC ACATTCCTTG TGACTGAGCA GGACGCTGGT
    CTTCTGCCCC GATGCAAAGA AAGGCGGGTC TTCCTTTTCG AACAAATTGT CATCTTTAGT
    GAGCCCTTAG ATAAAAAGAA AGGATACTCT ACACCTGGAT TTCTTTTTAA AAATAATATC
    AAAGTGAGCT GCCTTAGTTT AGAGGAGTGT GTTGATAGTG ATCCATGCAA GTTTGCACTA
    TTGTCAAGGT CTGCTGACTC TACAGAGATG TTTGTTTTGC ATTCTTCATC GCCTGGAGTG
    AGACAGTTAT GGATTCATGA AATCAACCAA ATTTTGGAAA ACCAGCGCAA TTTTTTAAAT
    GCCTTGACAT CACCAATTGA ATACCACAGA AATCACACTG GTGGAAGCAG TAGTGGCAAC
    GTCACCTTTA CCAGCAGTCC CAGCAATTGT AGTGGGCTGG GTGGCTCCAG CCGAAGCAGG
    CCCTCTCGGA TTCCCCAGCC TGTTAGACAC CACTCTCCAG TCCTGGTCTC CTCTGCAGCT
    TCGGCACAGT CAGAGGCAGA CAAGATGTCA GGTATGTCAG CTCGCCGTGC ACCAGTTGTA
    CCTCTCAACC TCTCCTTAGA CAATAACCCC AGCCAGAAAG GTAGAGTAAC TGCTGCTCCA
    GAGACAGGCG GGCGAGATAA AGAATCCAAT TCAGTGCCAA AGATGAAAAT GCTTGATGGC
    CCCAGGAAAT CAGCTAGTGG CATTCCAACC TTAGCTGATG GTGCACAGAA AGAATTGCGT
    GGGAGCACAG AAGACAGTCG AATTAGAAGC ACTATGGGCA CTCTAACCTT AGGAAAGCCA
    AGAAATGGTT CTGCCTCACC CCTCAACTCT CCTGTCACAA TTAATTTTCC ATCGTCTTTT
    GGTAAGGAAT CAGGGCCACC AAGCAGTCCC CTTCAGAAAG GCTCTTTTTG GAGCTCAATT
    CCAGGATCTC CAGCCAGCCG ACCTGGCTCC TTCACTTTCC CTGGAGACAG TGACTCTCTC
    CAGCGACAAG TTCATCGGCA CTCCACACAC AGCAAGGATA CAGACCGCAT GAGCACCTGC
    TCCTCGACTA GTGAGCAGTC CGTTCACTCC ACTCAGAGTA ATGGGGTAAG GACAGAGAGT
    GAAAGTAGCA GCAGTAGCAA TATCTCCACC ATGCTGGTTA CGCACGATTA TACAGCGGTG
    AAGGAGGATG AGATCAATGT CTACCAGGGA GAGGTTGTAC AAATTCTGGC AAGTAACCAA
    CAGAACATGT TTCTGGTGTT CAGAGCTGCC ACTGATGAGT GCCCTGCAGC TGAGGGCTGG
    ATTCCAAGCT ATGTTCTGGG TCACACAAGT GCAATCATCA CTGATATGAC TGATGCAACT
    ATGAAGAAGT CCTCTTCTTG GCATACAGCC CTTAGAATAA GAAAGAAATC TGAGAAGAGG
    GAAAAAGATG GCAGCAGAGA CACAAAACTA GAGAATGGTT ATCGCAAATC AAGAGAAGGA
    CTAGCTAATA AGGTATCAGT TAAGCTCCTG AATCCAAACT ACATTTATGA TGCTCCTCCA
    GAGTTTATTG TACCACTGAA CGAGGTTACA TGTGACACAG GAGAGACTGT GATTATTCGA
    TGCAAAGTTT GTGGTCGCCC CAAGGCATCC ATTACATGGA AAGGTCCCGA CCATAATACT
    TTGAGCAACG ATGGACACTA CAGTATATCC TACAGTGATC TTGGAGAAGC AACTCTAAAA
    ATAGTTGGTG TGACCACTGA GGATGATGGT ATATACACCT GCATTGCAAC AAATGACTTG
    GGTTCAGTAT CCGCTTCCTC CAATCTTAGA GTGTTAGGTT CATGGAGTGA TGGACTGAGA
    GTAATCTGGA AAGAAAACTT CGACATTGCT TATAGTGAAG TGGCTGAACT TGGCAGGGGC
    AGGTTTTCTG TCGTGAAAAA ATGTGAACAA AGAGTTACAA GACGCATAGT AGCCACCAAA
    TTTGTTAACA AGAAGTTGAT GAAAAGAGAC CAGGTTACCC ATGAACTTGG AATTCTGCAG
    AGCCTTCAGC ACCCACAACT GATCAGCCTT CTTGACACTT TTGAGACACC AGCAAGCTAT
    GTCTTAGTAC TTGAGATAGC TGATCAGGGA TGTTTGTTAG AATATGTGGT ACAGTGGGGA
    AACCTTACAG AAGAAAAGAT CACCTCATAT ATGAGAGAGG TGCTGGAGGC CCTGCAGTAT
    CTTCATAATT GCAGAATAGC ACATCTGGAC CTAAAGCCTG AGAACATTCT TGTGGATCAG
    AGCCTAACCA AACCAACTAT AAAACTGTCA GACTTTGGCG ATGCTGTTCA GCTTAATACC
    ACTTACTACA TTCACCAATT GTTGGGCAGT GCAGAGTTTG CCGCTCCAGA GATCATCCTT
    GGGAATCCTG TCTCCCTTAC CTCTGATATA TGGAGTATTG GAGTTCTTAC TTATGTCCTT
    CTCAGTGGCG TCTCTCCTTT CCTTGATGAA AGTGTTGAAG AGACATGCCT GAACATTTGC
    CGACTAGACT TTAGTTTTCC CGATGACTAC TTTAAGGGAG TAAGTCAGAA GGCCAAAGAA
    TTTGTATGTT TCTTACTGCA GGGTGACCAG ACAAAGCGCC CTTCTGCTGC TGTATGTCTA
    AAGGAACAGT GGTTGCAGCC AGGCCAGCAC AAACGTAGTG GCACATTAGA CATGTCAAGA
    CTGACCTCGT TTATTGAACG CCGTAAACAC CAGAATGATG TTCGACCCAT TGGTAGCATT
    AAAAACTTCC TTCAGAGTCG CCTTTTACCC AGAATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031759101.1
    CDS170..9328
    Translation

    Target ORF information:

    RefSeq Version XM_031903241.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031903241.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGCAGACTC ATCGCTGGAA AGATGACGGA GAAACAACAT TAGGTTTTCG TAGAAATGAT 
    GAATTGAAAG CAATGGAGGT GTTGCCAATT TTAAAAGAAA AAGTGGCATA TCTATCAGGT
    GGCAGAGATA AACGAGGTGG TCCTGTATTA ACATTTCCAG CACGCAGCAA CCATGATCGA
    ATACGACAAG AGGATCTCCG AAGACTGATT TCATATCTTG CTTGTATTCC TAGTGAGGAG
    GTGTGCAAGC GAGGCTTCAC AGTGATTGTA GATATGCGTG GTTCCAAGTG GGATTCTATT
    AAACCCTTGC TGAAAATTTT ACAAGAATCC TTTCCATGCT GTATTCATGT TGCACTAATT
    ATCAAACCAG ATAACTTCTG GCAGAAGCAA AGAACTAATT TTGGAAGTTC TAAGTTTGAA
    TTTGAGACAA GTATGGTGTC TTTAGAAGGC CTTACCAAAG TAGTTGACCC TTCCCAGCTA
    ACTCCAGAAT TTGAGGGTTG CTTGGAATAC AACCATGAAG AGTGGATAGA GATCAGAGTA
    GCATTTGAGG ATTTTATTAA TAATGCAACT CATATGCTGT CTCGATTAGA GGAATTACAG
    GAGATGCTTG CAATTAAAGA GTTGCCTCAG GATTTAGAGG GGGCACGCAA TATGATTGAA
    GAACATTCTC AAATGAAAAA AAGGGTTATA AAGGCTCCTA TTGAAGATCT TGATTTAGAA
    GGGCAAAAGC TTTTACAAAC AATACAAAGC AGTGATAGCT TTCCTAAGAA GAACTCTAGT
    TCTGGAAATG CAGACCTTCA AAATCTTCTT CCCAAAGTTT CCACAATGTT AGACAGGCTT
    CATTCAACAC GACAACATCT TCATCAGATG TGGCACATGC GAAAAGTAAA ACTGGACCAG
    TGCTTTCAGC TAAGGTTGTT TGAGCAGGAT GCTGAGAAGA TGTTTGACTG GATTACACAC
    AACAAAGGTC TGTTTCTGAC AAGTTATACT GACATTGGCA CCCGTAATAA TCATGCTATG
    GAACTTCAGA CACAGCACAA TCATTTTGCT ATGAACTGCA TGAATGTCTA TGTAAACATT
    AGCCGAATAA TGTCTGTGGC AAATCGACTA GTAGAATCTG GACACTATGC CTCACAGCAA
    ATTAAGCAGA TAGCTAACCA GTTGGAGCAA GAGTGGAAGG CTTTTGCCGC AGCCCTTGAT
    GAACGTAGTA CGTTGCTTGA CATGTCTGCA ATGTTCCATC AAAAAGCGGA GCAGTACATG
    GGCAATGTGG ATTCATGGTG CAAAGCTTGT GGGGAAGTGG ATCTTCCATC AGAGCTGCAG
    GATCTAGAAG ATGCCATTCA TCACCATCAG GGAATATATG AGCATATCAC TTTAGCATAT
    TCAGAGGTTA GCCAGGATGG TAAATCTTTA TTAGACAAAC TCCAGAGACC ATTGACTCCT
    GGTAGTTCTG ACTCACTGAC AGCTTCTGCC AACTATTCAA AGGCTGTGCA TCACGTCTTA
    GATGCAATAC ATGAAGTCTT GCACCACCAG CGTCAGTTGG AAAACATCTG GCAACATCGC
    AAAGTTCGTC TGCATCAACG CCTACAGCTC TGTGTTTTTC AGCAAGATGT TCAGCAGGTC
    TTGGATTGGA TAGAGAACCA TGGTGAAGCC TTTCTCAGCA AACACACTGG GGTAGGCAAG
    TCTCTGCACA GGGCCAGAGC CTTGCAAAAG CGACATGAAG ACTTTGAAGA AGTCGCTCAG
    AATACATACA CCAATGCTGA TAAGCTGCTG GAAGCTGCAG AACAGTTGGC CCAGACAGGA
    GAGTGCGATC CAGAGGAAAT ATACCAGGCT GCTCATCAGC TTGAGGATCG GATTCAGGAT
    TTTGTAAGAC GTGTAGAACA AAGAAAAGTT CTCTTGGACA TGTCTGTATC ATTCCACTCG
    CATGTTAAAG AGCTATGGAC ATGGCTGGAG GACCTACAAA AGGAACTGCT GGATGATGTC
    TATGCGGAGT CAGTAGAAGC TGTACAGGAC TTGATCAAAC GGTTTGGACA ACAGCAGCAG
    ACAACCCTCC AGTTTACAGT AAATGTCATC AAAGAAGGGG AGGATCTAAT TCAGCAACTA
    AGAGATTCTG CTATATCCAG CAACAAAACT CCTCACAACA GCTCAATCAA TCACATTGAG
    ACTGTCCTTC AACAACTTGA TGAGGCCCAA TCACAGATGG AGGAGCTTTT CCAAGAGAGA
    AAAATCAAAC TTGAGCTATT CTTGCAGCTT CGCATCTTTG AGAGAGATGC CATTGATATC
    ATCTCAGACT TGGAATCATG GAATGATGAA CTTTCTCAGC AAATGAATGA TTTTGACACA
    GAGGACCTCA CACTAGCCGA GCAAAGGCTA CAGCATCACG CAGACAAAGC TCTGACAATG
    AACAACCTGA CATTTGATGT CATCCACCAA GGACAAGAGT TACTACAATA TGTGAATGAA
    GTTCAGAACT CAGGGGTTGA GCTACTCTGT GATAGAGATG TAGATATGGC TACTAGAGTT
    CAGGACCTGT TGGAATTCCT CCATGAGAAA CAACAGGAGT TGGATCTAGC AGCTGAGCAG
    CACCGAAAAC ATCTAGAACA ATGTGTGCAA CTACGACATT TGCAAGCTGA AGTCAAACAG
    GTACTCGGCT GGATACGGAA TGGAGAGTCT ATGTTAAATG CCGGGCTTAT CACTGCCAGT
    TCATTGCAAG AAGCAGAGCA ACTTCAAAGG GAACATGAGC AGTTCCAGCA TGCAATAGAA
    AAAACCCACC AGAGTGCTCT GCAGGTGCAG CAGAAGGCAG AGGCCATGCT TCAAGCAAAC
    CATTACGACA TGGATATGAT CCGGGACTGT GCTGAAAAAG TGGCCTCTCA TTGGCAACAG
    TTGATGCTTA AAATGGAAGA CCGTCTTAAG CTTGTCAATG CCTCTGTGGC CTTTTATAAA
    ACCTCAGAGC AGGTATGCAG TGTTCTAGAG AGCCTAGAGC AGGAGTACAA GCGGGAGGAA
    GATTGGTGTG GCGGAGCTGA TAAACTTGGG CCTAACTCAG AGACTGACCA TGTTACTCCT
    ATGATCAGCA AACACCTTGA ACAAAAGGAA GCCTTTCTTA AGGCCTGCAC TTTGGCTCGC
    AGAAATGCTG ATGTCTTTCT GAAATACTTG CACAGGAATA GTGTCAATAT GCCTGGAATG
    GTGACACATG TAAAGGCTCC TGAACAGCAA GTAAAAAATA TTTTAAATGA GCTGTTTCAG
    AGGGAAAATA GAGTTCTTCA TTATTGGACC TTGCGGAAAA GGAGACTAGA CCATTGCCAG
    CAGTATGTAG TATTTGAAAG AAGTGCCAAA CAGGCTCTGG AGTGGATCCA AGATAATGGA
    GAATTTTACA TTTCCACACA CACCTCCACT GGCTCCACAA TTCAGCATAC ACAAGAACTC
    TTAAAAGAGC ATGAGGAGTT TCAGATCACA GCAAAGCAAA CCAAAGAGAG GGTAAAGCTG
    TTGATACAGC TGGCTGATGG CTTTTGTGAA AAAGGGCATG CTCATGCTGC AGAAATTAAA
    AAATGTGTTA CAGCGGTGGA CAAGAGGTAC AGAGACTTCT CGCTGCGTAT GGAAAAGTAC
    AGAACCAGCT TAGAGAAGGC TTTGGGCATT TCTTCTGATT CCAAGTCGAG TAAAAGCCTT
    CAGTTGGATA TTATTCCAGC CACTGCCCCT GGTTCCGAAG TCAAGCTTCG TGATGCAACA
    CATGAACTCA ATGAGGAGAA AAGAAAATCT GCTCGTCGAA AAGAATTCAT CATGGCAGAA
    CTTATACAAA CTGAAAAAGC ATATGTTCGT GATCTTCGGG AATGCATGGA TACATATCTG
    TGGGAAATGA CAAGCGGAGT TGAAGAGATC CCACCAGGCA TTGTAAATAA AGAACACATT
    ATCTTTGGAA ACATGCAGGA AATATATGAA TTTCATAACA ACATATTCTT AAAGGAGCTT
    GAGAAGTATG AGCAGCTACC TGAAGATGTT GGACATTGTT TTGTCACATG GGCTGACAAA
    TTTCAGATGT ATGTGTCCTA CTGTAAGAAT AAGCCTGATT CAACACAACT TATCTTGGAA
    CAAGCAGGAA CATATTTTGA TGAAATTCAG CAGCGCCATG AGCTGGTCAA CTCCATCTCT
    TCTTATCTTA TCAAACCTGT GCAAAGGATA ACAAAATATC AGCTTCTTTT AAAAGAGCTG
    CTGACATGCT GCGAAGAAGG TAAAGGAGAG ATTAAAGATG GGCTGGAAGT GATGCTAAGT
    GTACCAAAAA GAGCCAACGA TGCAATGCAT CTCAGCATGC TTGAAGGGTT TGATGAGAAC
    ATTGAATCTC AGGGAGAACT TATTCTGCAA GAGTCTTTTC AAGTGTGGGA TCCTAAAACC
    TTAATAAGAA AGGGGCGTGA GAGACATCTC TTCTTGTTTG AAATGTCTTT GGTCTTCAGC
    AAAGAAGTGA AAGATTCAAG TGGAAGGAGC AAATATATTT ACAAATCAAA ATTATTTACC
    TCAGAGCTTG GAGTCACAGA ACATGTAGAA GGTGATCCTT GCAAATTTGC ACTTTGGGTA
    GGCAGAACGC CAACATCTGA TAACAAAATA GTCCTTAAGG GCTCAAGTAT TGAGAACAAG
    CAAGATTGGA TCAAGCATAT AAGAGAAGTG ATCCAAGACC GAACCATTCA CCTGAGAGGC
    GCATTAAAAG AGCCTATTCA TATACCTAAA ACAGCTGCTG CTACAAAACA GAAACCTGCC
    AAAAGAGATG GAGAAGACCT GGACAGTCAA GGAGATGGCA GCAGCCAACC TGATACAATA
    TCTATTGCTT CACGAACTTC ACAGAACACA TTAGACAGTG ATAAGCTTTC TGGTGGATGT
    GAGCTGACAG TAGTCATCCA TGACTTTACT GCCTGTAATA GCAATGAGCT GACAATTAAA
    CGAGGGCAGA CTGTGGAGGT TCTAGAGCGG CATCACGATC GACCTGACTG GTGTTTAGTT
    CGGACTACTG ACCGGTCACC TGCAGCAGAG GGCTTGGTGC CTAGTAGTTC ACTGTGTATT
    GCACATTCTA GAAGTAGCAT GGAAATGGAA GGCATATTTA ACCACAAAGA TTCCCTCTCT
    GTCTCCAGTA ATGATGCCAG TCCTCCTGCT TCAGTAATTT CCCTTCAGCC TCACATGATT
    GGTGCTCAAA GCTCTCCTGG CCCAAAGCGA CCTGGCAATA CTTTGAGAAA ATGGCTCACC
    AGCCCTGTCA GGAGACTTAG CAGTGGCAAA GCTGATGGAC ATGTGAAAAA ATTGGCTCAT
    AAACACAAAA AAAGCAGGGA GGTTCGTAAA AACACAGACT CAGGTTCCCA AAAGGACTCT
    GATGACAGTG CAGCAACACC TCAAGATGAA ACTATCGAGG AGAGAGTGCG CAATGAAGGA
    CTTAGCAGTG GTACGCTTTC TAAATCCTCC TCGTCTGGCA TGCAAAGCTG TGGAGAAGAA
    GAGGGCGAGG AAGGACCTGA TGCAGTTCCA TTGCCTCCAC CCATGGCTAT TCAGCAGCAC
    AGCCTTCTTC AGCCTGACTC ACAGGATGAC AAGACATCAT CTCGGTTGCT GGTGCGACCT
    ACTAGCTCAG AGACACCTAG TGCTGCAGAG CTTGTTAGTG CTATTGAAGA ACTTGTAAAG
    AGTAAAATGG CTTTGGAAGA CCGGCCAAGT TCACTTCAAG TAGACCAGGG AGACAGTAGT
    AGCCCATCAT TCAACCCTTC AGATACATCT CTGCTTTCCT CCTCTTCTCC AATTGATGAA
    ATGGAAGAAA AAAAATCAAG CTCTTTAAAA AGAAGGCACT ATGTATTACA AGAGCTAGTA
    GAAACTGAAC GCGATTATGT TCGGGACCTT GGCTATGTGG TTGAGGGTTA TATGGCACTT
    ATGAAGGAGG ATGGAGTTCC AGATGATATG AAAGGAAAAG ACAAGATTGT TTTTGGCAAC
    TTACACCAGA TCTACGAGTG GCACAGAGAC TTTTTTTTGG GTGAACTGGA AAGGTGCCTG
    GATGAGCCTG AAAGATTGGC ATCCCTATTT GTCAAGCATG AAAGAAGATT GCATATGTAC
    ATAGTTTACT GCCAAAATAA ACCAAAGTCT GAACATATTG TTTCAGAATA CATTGATACC
    TATTTTGAGG ACCTGAAACA GCGTCTGGGC CATCGACTTC AGCTTACAGA TCTGTTAATA
    AAACCCATAC AAAGAATAAT GAAATATCAG CTGCTATTAA AGGATTTTCT CAAGTACTCT
    AAGAAGGCTT GCATAGATGT AGCAGAACTT GAGAGAGCTG TGGAAGTCAT GTGTATCGTA
    CCGAAGCGGT GTAATGACAT GATGAATGTT GGTCGTTTGC AAGGATTTGA TGGCAAAATA
    GTTGCCCAAG GAAAGCTGCT ACTGCAAGAC ACATTCCTTG TGACTGAGCA GGACGCTGGT
    CTTCTGCCCC GATGCAAAGA AAGGCGGGTC TTCCTTTTCG AACAAATTGT CATCTTTAGT
    GAGCCCTTAG ATAAAAAGAA AGGATACTCT ACACCTGGAT TTCTTTTTAA AAATAATATC
    AAAGTGAGCT GCCTTAGTTT AGAGGAGTGT GTTGATAGTG ATCCATGCAA GTTTGCACTA
    TTGTCAAGGT CTGCTGACTC TACAGAGATG TTTGTTTTGC ATTCTTCATC GCCTGGAGTG
    AGACAGTTAT GGATTCATGA AATCAACCAA ATTTTGGAAA ACCAGCGCAA TTTTTTAAAT
    GCCTTGACAT CACCAATTGA ATACCACAGA AATCACACTG GTGGAAGCAG TAGTGGCAAC
    GTCACCTTTA CCAGCAGTCC CAGCAATTGT AGTGGGCTGG GTGGCTCCAG CCGAAGCAGG
    CCCTCTCGGA TTCCCCAGCC TGTTAGACAC CACTCTCCAG TCCTGGTCTC CTCTGCAGCT
    TCGGCACAGT CAGAGGCAGA CAAGATGTCA GGTATGTCAG CTCGCCGTGC ACCAGTTGTA
    CCTCTCAACC TCTCCTTAGA CAATAACCCC AGCCAGAAAG GTAGAGTAAC TGCTGCTCCA
    GAGACAGGCG GGCGAGATAA AGAATCCAAT TCAGTGCCAA AGATGAAAAT GCTTGATGGC
    CCCAGGAAAT CAGCTAGTGG CATTCCAACC TTAGCTGATG GTGCACAGAA AGAATTGCGT
    GGGAGCACAG AAGACAGTCG AATTAGAAGC ACTATGGGCA CTCTAACCTT AGGAAAGCCA
    AGAAATGGTT CTGCCTCACC CCTCAACTCT CCTGTCACAA TTAATTTTCC ATCGTCTTTT
    GGTAAGGAAT CAGGGCCACC AAGCAGTCCC CTTCAGAAAG GCTCTTTTTG GAGCTCAATT
    CCAGGATCTC CAGCCAGCCG ACCTGGCTCC TTCACTTTCC CTGGAGACAG TGACTCTCTC
    CAGCGACAAG TTCATCGGCA CTCCACACAC AGCAAGGATA CAGACCGCAT GAGCACCTGC
    TCCTCGACTA GTGAGCAGTC CGTTCACTCC ACTCAGAGTA ATGGGGTAAG GACAGAGAGT
    GAAAGTAGCA GCAGTAGCAA TATCTCCACC ATGCTGGTTA CGCACGATTA TACAGCGGTG
    AAGGAGGATG AGATCAATGT CTACCAGGGA GAGGTTGTAC AAATTCTGGC AAGTAACCAA
    CAGAACATGT TTCTGGTGTT CAGAGCTGCC ACTGATGAGT GCCCTGCAGC TGAGGGCTGG
    ATTCCAAGCT ATGTTCTGGG TCACACAAGT GCAATCATCA CTGATATGAC TGATGCAACT
    ATGAAGAAGT CCTCTTCTTG GCATACAGCC CTTAGAATAA GAAAGAAATC TGAGAAGAGG
    GAAAAAGATG GCAGCAGAGA CACAAAACTA GAGAATGGTT ATCGCAAATC AAGAGAAGGA
    CTAGCTAATA AGGTATCAGT TAAGCTCCTG AATCCAAACT ACATTTATGA TGCTCCTCCA
    GAGTTTATTG TACCACTGAA CGAGGTTACA TGTGACACAG GAGAGACTGT GATTATTCGA
    TGCAAAGTTT GTGGTCGCCC CAAGGCATCC ATTACATGGA AAGGTCCCGA CCATAATACT
    TTGAGCAACG ATGGACACTA CAGTATATCC TACAGTGATC TTGGAGAAGC AACTCTAAAA
    ATAGTTGGTG TGACCACTGA GGATGATGGT ATATACACCT GCATTGCAAC AAATGACTTG
    GGTTCAGTAT CCGCTTCCTC CAATCTTAGA GTGTTAGGTT CATGGAGTGA TGGACTGAGA
    GTAATCTGGA AAGAAAACTT CGACATTGCT TATAGTGAAG TGGCTGAACT TGGCAGGGGC
    AGGTTTTCTG TCGTGAAAAA ATGTGAACAA AGAGTTACAA GACGCATAGT AGCCACCAAA
    TTTGTTAACA AGAAGTTGAT GAAAAGAGAC CAGGTTACCC ATGAACTTGG AATTCTGCAG
    AGCCTTCAGC ACCCACAACT GATCAGCCTT CTTGACACTT TTGAGACACC AGCAAGCTAT
    GTCTTAGTAC TTGAGATAGC TGATCAGGGA TGTTTGTTAG AATATGTGGT ACAGTGGGGA
    AACCTTACAG AAGAAAAGAT CACCTCATAT ATGAGAGAGG TGCTGGAGGC CCTGCAGTAT
    CTTCATAATT GCAGAATAGC ACATCTGGAC CTAAAGCCTG AGAACATTCT TGTGGATCAG
    AGCCTAACCA AACCAACTAT AAAACTGTCA GACTTTGGCG ATGCTGTTCA GCTTAATACC
    ACTTACTACA TTCACCAATT GTTGGGCAGT GCAGAGTTTG CCGCTCCAGA GATCATCCTT
    GGGAATCCTG TCTCCCTTAC CTCTGATATA TGGAGTATTG GAGTTCTTAC TTATGTCCTT
    CTCAGTGGCG TCTCTCCTTT CCTTGATGAA AGTGTTGAAG AGACATGCCT GAACATTTGC
    CGACTAGACT TTAGTTTTCC CGATGACTAC TTTAAGGGAG TAAGTCAGAA GGCCAAAGAA
    TTTGTATGTT TCTTACTGCA GGGTGACCAG ACAAAGCGCC CTTCTGCTGC TGTATGTCTA
    AAGGAACAGT GGTTGCAGCC AGGCCAGCAC AAACGTAGTG GCACATTAGA CATGTCAAGA
    CTGACCTCGT TTATTGAACG CCGTAAACAC CAGAATGATG TTCGACCCAT TGGTAGCATT
    AAAAACTTCC TTCAGAGTCG CCTTTTACCC AGAATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.