efcab5 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following efcab5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the efcab5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa32213 XM_012957531.2
Latest version!
Xenopus tropicalis EF-hand calcium binding domain 5 (efcab5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa52053 XM_031896666.1
Latest version!
Xenopus tropicalis EF-hand calcium binding domain 5 (efcab5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa32213
    Clone ID Related Accession (Same CDS sequence) XM_012957531.2
    Accession Version XM_012957531.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3564bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957531.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGATGCAG AAAGTGATTA CAGTGCTCAC ACCTCCTCCC TGGACTGTAG TAGTGAAAAA 
    CTAGAAAGCA ACCCAAAAAA CAATGCCATT CAGACTCAAG ACAGAGGTGG TGACTGGAGA
    ACAATATTTT TTGACAAAAT GCAAGAAAGA GGATTAAAGC TACAACAGAA GACCTTGGAA
    AGTCTAAAAC TCCAGCAAGC ACAAGAAAAG AAAGAAAAAA AGGCAAATCC TCCAGATGAA
    CAGGCCAAAG ATTGGTTTAG CCAGGATAAA ACAACTGTGC ATACCAGAGC CTACTTACTT
    GACAAGCTGA TGCCTTCCAT TGTACCGGGG CTGGAAGCTC TACTTAAAGA ATTAGAGAAA
    AGAAAGGTTC TAGAAGACAG TTCCATTGCA TTTGATCCAA TTATATATCT TGGAGAGCAG
    CTCATGAGGC ATAATCCAGA GTATGTTAGG GATCTTCAAA GCAATTCATA TGTACAAGGG
    CTAAAAGAGG TTCTTTCTGA TCTCAAAGAA CAGATTTTTG ATCTAGAATT TAACAGACTT
    GGTCAACTAA AGGAAGAAAT GAGGGAAGTC CAGCACCAGA GAGCACAAAT AGAAAGCATC
    CAATCCCAGG TGAAGCATGA GAGGCGAGAA GTTCTGGCTA TGCAGTTCCA AGAATGGACT
    TTGGATGTCA GTGGAAGGAT CCCTCTTGTG CTGGTACAAA GTGCATTAAA ATCTTTTTCG
    GAAGTAATAG CTAACCTTCC ATTGGATGAA AAAGCAGCTT ATGAAGAGGA GCTGAAGACG
    GTGAACACAA TGGAATGTAT GCTAACTCAG GAAGAATTTG TGGAGTATGT GCATCCATTT
    ATCTGTCACT TCAGTACAGA CTTATTTCAG GAGTTCCTGC ATCATCTCTC ACAGTGTGCA
    GATACATTTC GTGAAACCAT TCGACATGAC ATCTGGAGAC AGATGTTTTC TGATCTCTTC
    TTTGCATGTG ACCCAGGAAA GGTTGGCTTT CTTGACAGAC AGAGAGTCCT GGCTTTACTG
    GAAACTTTCT ATGAAAAAAC TATTTCAGAA ACTGATGAAT GGCAGCCACG AAACCCTAGG
    CAATGGCCAG TGGTTGAGCT GGATGAAATG GATCCAACAG ACTTTTGGGC TGACTTCCAA
    GAAGGAGTAA AGTCTTTAGA GGAACAAGAA GGTTCCGTTA TGGCAATCTC AGCAACGACT
    CAAATATCAA CTCCAACAGA AGGCGATTAT CATTTAATAG GCCCTATGCA AGAACAAGAT
    AAGCTAGATG GAATTCCTTC AACTGAAGGC AGTAGTGAAT CACATCTTAT TGGTTTGCCA
    TTCCCAACAA AGTCAGAGGC ACCTACTGCT GAAGACATTA GGAGTAGTGA CTCATATCTC
    ATTGATGGAA AACCTTGGTC AGGAAAACTT CTGACTGCTG ATCTGGCCAT TAAGTACAGC
    TCTTACGGTG ACAAAATCCA AGATGAGTGC AATGCAGCAG CTTCTAAATT CACAGATTTG
    CACCCTATCA TGTCAGAGAT TAACTCCCGA GGCCCATCAT GCATTGCAAG TGCGTTCAAT
    GACAGTGCAT TAAATTTACC CCAGTTTGTA CAATTGATGG AGACTTTCCT GGGGGAAGGG
    GCACCACACT CATTTGTGGA GAAGCTTGTA TCCTTTATAC AGAAGGAATA TGCTGAGACA
    GAAGAGGAGA AGGTGGCATT GTTGTCAAAA GTTCGACAAG ATGCCTTACT GGCACGACAA
    AAGTTGGTGC TGCAAGCTCT CTTTGTGAAG TGGGACAATG ATGGATCTGG GTTTCTGGGA
    CTAGATGAGG TTGAGGCTCT GCTGTTCAAG TACAAGGAAG GCATGGAAAC TGAGGTGATG
    GCAAGAGGAA GGGAAGCACT GAGTTCCTCC CAATCTCAAA GGAGCATTCA GAGGCTCTCG
    GCTCCTGAAT TCTGCAAATA CATTCAGGGT GTGGTGAGGC AGCTGCCTGG TCCTGATGAG
    GAGGTGTTTG AAAGCTTGGT GGAATACCTG ACATCCAGTG TGGAACGTTC TCAGGCTGAG
    AAGCTGCGAG GGGCTTCCAG AAGAAAGTGG TTGCAGCAAA TCCAAGTGGC AGCTGAGACA
    AGTGGAGGAA ATCTGCAGCC TGTGTACAAA GCTCTGTTTC AGGCACTTTT TAAGGATGCA
    GAGGCCCATG GAAATAACAA AAGGATTAGT GCTAACATTG GCCTTCTGGA GGAGAGCTCA
    GTTGGTGGAG GATGGACACA GATCATCCGT TATGTAGCTT GTACTGCAGA TGATACTCCG
    TATGTACTAA ACAAGACCCT ACAAAGAGAT ATGAAGGGTG TGAGCTTTGC AGCAGTGGAG
    AGCGGAATAC CACAGCATGT GCCTCGGGTT CAGTACCACG GACACATCCA TTTCTGGAAT
    ACAGACCGAC CAGAAGAAGA GCGCAAAGGG TCTCTTATTG TCCTGCCTCT CATTGATATG
    CAACAACGGG CATTTGGTAC CCTTTCCATT GATACAATGA GAGATCCCCG GGAAAGGAAT
    ATATTTCTCA CTCATGAGAT CAGCTTTTAT CAGGGGGTCA CCAATGCATT CAGTGCATCA
    TATAATCACG TGCAACAAAT CAAGGATATT CTTCAACTAA TAAGCAGTGC CCTTGCCTGG
    ATCTACAGCC GTGCTCCCAA CATCCGCAGC ATCACCACAT ACCTAATGGA GCCCAGTGTA
    GATAAGTTAC AGGGTTATGT GTTTCGCAAA ATGATGAGCA CAGATAATAA CACAGGGCTG
    TCTGAAATCT ACAGCTCCCC AGCCATGCTG CGCAGGCAGG ATAATCTTTT CAGGGATTAC
    CTCTTTAAGT GTGCCGATAG CTCAGAAGTT ATTACAACAG ATGCCTATGG AGAGCGTCAC
    ATTGCTGTAC CTATTCGCAA CTCAGCGGGA CGGGCACTTG GAGTGCTGGA TCTAAGCACT
    GGGCAGTGCA AGGAGCTACC TGCACACGAG TATCAAGATC TGCAAAAAAT GCTGCAAATG
    CTGCAGGAAG CTTGCTATGA AATCCTAGAG GAGAACCAAT TCAGCGACAC CCAGAAAAAG
    TCTATCCTAG AGGCAGAGCG AGTGTCTGGG CACAGACGGG CAGCGGTTCT GTTCCATCGC
    TTCATGCTGC AGGATCTGCG TCAGTGTGTG AGCAAACTCG ACCACCAGTC TTTTGCTGAG
    CTCAAGAGTT ACAAGGAACC CCCTACCATG GTGCATAGTA TCCTCAAAGC TGTGCTGCTC
    CTCTTCTGCC CTGACTGGGC CGAATCAGAT GAAGTTGAAA GCTGGAATCA GTGTAAACTG
    AAAGTTAACA GCGATTTGAT CAGGAAAATT TTATTCTTTG ACCCAACTGC CCAATCTGTG
    CAGATTAAAC CAGAGATGCT GGCCAAGTAT ATCAAAGGTA TTCCCAGGGG TGCAGTTTGG
    AAACATGGCT CCATCCCTGC TGAACATCTA TATAACTGGG CCTTCACCTG TCTCTCCCTA
    ATGGAACTTA CAGAGAAAAT CCCAAATACA CAGAGTCCAT CACTGATCAC TGTGCCAGTA
    CCAAACAGAA GTGAAAACGC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012812985.1
    CDS190..3753
    Translation

    Target ORF information:

    RefSeq Version XM_012957531.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis EF-hand calcium binding domain 5 (efcab5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957531.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGATGCAG AAAGTGATTA CAGTGCTCAC ACCTCCTCCC TGGACTGTAG TAGTGAAAAA 
    CTAGAAAGCA ACCCAAAAAA CAATGCCATT CAGACTCAAG ACAGAGGTGG TGACTGGAGA
    ACAATATTTT TTGACAAAAT GCAAGAAAGA GGATTAAAGC TACAACAGAA GACCTTGGAA
    AGTCTAAAAC TCCAGCAAGC ACAAGAAAAG AAAGAAAAAA AGGCAAATCC TCCAGATGAA
    CAGGCCAAAG ATTGGTTTAG CCAGGATAAA ACAACTGTGC ATACCAGAGC CTACTTACTT
    GACAAGCTGA TGCCTTCCAT TGTACCGGGG CTGGAAGCTC TACTTAAAGA ATTAGAGAAA
    AGAAAGGTTC TAGAAGACAG TTCCATTGCA TTTGATCCAA TTATATATCT TGGAGAGCAG
    CTCATGAGGC ATAATCCAGA GTATGTTAGG GATCTTCAAA GCAATTCATA TGTACAAGGG
    CTAAAAGAGG TTCTTTCTGA TCTCAAAGAA CAGATTTTTG ATCTAGAATT TAACAGACTT
    GGTCAACTAA AGGAAGAAAT GAGGGAAGTC CAGCACCAGA GAGCACAAAT AGAAAGCATC
    CAATCCCAGG TGAAGCATGA GAGGCGAGAA GTTCTGGCTA TGCAGTTCCA AGAATGGACT
    TTGGATGTCA GTGGAAGGAT CCCTCTTGTG CTGGTACAAA GTGCATTAAA ATCTTTTTCG
    GAAGTAATAG CTAACCTTCC ATTGGATGAA AAAGCAGCTT ATGAAGAGGA GCTGAAGACG
    GTGAACACAA TGGAATGTAT GCTAACTCAG GAAGAATTTG TGGAGTATGT GCATCCATTT
    ATCTGTCACT TCAGTACAGA CTTATTTCAG GAGTTCCTGC ATCATCTCTC ACAGTGTGCA
    GATACATTTC GTGAAACCAT TCGACATGAC ATCTGGAGAC AGATGTTTTC TGATCTCTTC
    TTTGCATGTG ACCCAGGAAA GGTTGGCTTT CTTGACAGAC AGAGAGTCCT GGCTTTACTG
    GAAACTTTCT ATGAAAAAAC TATTTCAGAA ACTGATGAAT GGCAGCCACG AAACCCTAGG
    CAATGGCCAG TGGTTGAGCT GGATGAAATG GATCCAACAG ACTTTTGGGC TGACTTCCAA
    GAAGGAGTAA AGTCTTTAGA GGAACAAGAA GGTTCCGTTA TGGCAATCTC AGCAACGACT
    CAAATATCAA CTCCAACAGA AGGCGATTAT CATTTAATAG GCCCTATGCA AGAACAAGAT
    AAGCTAGATG GAATTCCTTC AACTGAAGGC AGTAGTGAAT CACATCTTAT TGGTTTGCCA
    TTCCCAACAA AGTCAGAGGC ACCTACTGCT GAAGACATTA GGAGTAGTGA CTCATATCTC
    ATTGATGGAA AACCTTGGTC AGGAAAACTT CTGACTGCTG ATCTGGCCAT TAAGTACAGC
    TCTTACGGTG ACAAAATCCA AGATGAGTGC AATGCAGCAG CTTCTAAATT CACAGATTTG
    CACCCTATCA TGTCAGAGAT TAACTCCCGA GGCCCATCAT GCATTGCAAG TGCGTTCAAT
    GACAGTGCAT TAAATTTACC CCAGTTTGTA CAATTGATGG AGACTTTCCT GGGGGAAGGG
    GCACCACACT CATTTGTGGA GAAGCTTGTA TCCTTTATAC AGAAGGAATA TGCTGAGACA
    GAAGAGGAGA AGGTGGCATT GTTGTCAAAA GTTCGACAAG ATGCCTTACT GGCACGACAA
    AAGTTGGTGC TGCAAGCTCT CTTTGTGAAG TGGGACAATG ATGGATCTGG GTTTCTGGGA
    CTAGATGAGG TTGAGGCTCT GCTGTTCAAG TACAAGGAAG GCATGGAAAC TGAGGTGATG
    GCAAGAGGAA GGGAAGCACT GAGTTCCTCC CAATCTCAAA GGAGCATTCA GAGGCTCTCG
    GCTCCTGAAT TCTGCAAATA CATTCAGGGT GTGGTGAGGC AGCTGCCTGG TCCTGATGAG
    GAGGTGTTTG AAAGCTTGGT GGAATACCTG ACATCCAGTG TGGAACGTTC TCAGGCTGAG
    AAGCTGCGAG GGGCTTCCAG AAGAAAGTGG TTGCAGCAAA TCCAAGTGGC AGCTGAGACA
    AGTGGAGGAA ATCTGCAGCC TGTGTACAAA GCTCTGTTTC AGGCACTTTT TAAGGATGCA
    GAGGCCCATG GAAATAACAA AAGGATTAGT GCTAACATTG GCCTTCTGGA GGAGAGCTCA
    GTTGGTGGAG GATGGACACA GATCATCCGT TATGTAGCTT GTACTGCAGA TGATACTCCG
    TATGTACTAA ACAAGACCCT ACAAAGAGAT ATGAAGGGTG TGAGCTTTGC AGCAGTGGAG
    AGCGGAATAC CACAGCATGT GCCTCGGGTT CAGTACCACG GACACATCCA TTTCTGGAAT
    ACAGACCGAC CAGAAGAAGA GCGCAAAGGG TCTCTTATTG TCCTGCCTCT CATTGATATG
    CAACAACGGG CATTTGGTAC CCTTTCCATT GATACAATGA GAGATCCCCG GGAAAGGAAT
    ATATTTCTCA CTCATGAGAT CAGCTTTTAT CAGGGGGTCA CCAATGCATT CAGTGCATCA
    TATAATCACG TGCAACAAAT CAAGGATATT CTTCAACTAA TAAGCAGTGC CCTTGCCTGG
    ATCTACAGCC GTGCTCCCAA CATCCGCAGC ATCACCACAT ACCTAATGGA GCCCAGTGTA
    GATAAGTTAC AGGGTTATGT GTTTCGCAAA ATGATGAGCA CAGATAATAA CACAGGGCTG
    TCTGAAATCT ACAGCTCCCC AGCCATGCTG CGCAGGCAGG ATAATCTTTT CAGGGATTAC
    CTCTTTAAGT GTGCCGATAG CTCAGAAGTT ATTACAACAG ATGCCTATGG AGAGCGTCAC
    ATTGCTGTAC CTATTCGCAA CTCAGCGGGA CGGGCACTTG GAGTGCTGGA TCTAAGCACT
    GGGCAGTGCA AGGAGCTACC TGCACACGAG TATCAAGATC TGCAAAAAAT GCTGCAAATG
    CTGCAGGAAG CTTGCTATGA AATCCTAGAG GAGAACCAAT TCAGCGACAC CCAGAAAAAG
    TCTATCCTAG AGGCAGAGCG AGTGTCTGGG CACAGACGGG CAGCGGTTCT GTTCCATCGC
    TTCATGCTGC AGGATCTGCG TCAGTGTGTG AGCAAACTCG ACCACCAGTC TTTTGCTGAG
    CTCAAGAGTT ACAAGGAACC CCCTACCATG GTGCATAGTA TCCTCAAAGC TGTGCTGCTC
    CTCTTCTGCC CTGACTGGGC CGAATCAGAT GAAGTTGAAA GCTGGAATCA GTGTAAACTG
    AAAGTTAACA GCGATTTGAT CAGGAAAATT TTATTCTTTG ACCCAACTGC CCAATCTGTG
    CAGATTAAAC CAGAGATGCT GGCCAAGTAT ATCAAAGGTA TTCCCAGGGG TGCAGTTTGG
    AAACATGGCT CCATCCCTGC TGAACATCTA TATAACTGGG CCTTCACCTG TCTCTCCCTA
    ATGGAACTTA CAGAGAAAAT CCCAAATACA CAGAGTCCAT CACTGATCAC TGTGCCAGTA
    CCAAACAGAA GTGAAAACGC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52053
    Clone ID Related Accession (Same CDS sequence) XM_031896666.1
    Accession Version XM_031896666.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3558bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGATGCAG AAAGTGATTA CAGTGCTCAC ACCTCCTCCC TGGACTGTAG TAGTGAAAAA 
    CTAGAAAGCA ACCCAAAAAA CAATGCCATT CAGACTCAAG ACAGAGGTGG TGACTGGAGA
    ACAATATTTT TTGACAAAAT GCAAGAAAGA GGATTAAAGC TACAACAGAA GACCTTGGAA
    AGTCTAAAAC TCCAGCAAGC ACAAGAAAAG AAAGAAAAAA AGGCAAATCC TCCAGATGAA
    CAGGCCAAAG ATTGGTTTAG CCAGGATAAA ACAACTGTGC ATACCAGAGC CTACTTACTT
    GACAAGCTGA TGCCTTCCAT TGTACCGGGG CTGGAAGCTC TACTTAAAGA ATTAGAGAAA
    AGAAAGGTTC TAGAAGACAG TTCCATTGCA TTTGATCCAA TTATATATCT TGGAGAGCAG
    CTCATGAGGC ATAATCCAGA GTATGTTAGG GATCTTCAAA GCAATTCATA TGTACAAGGG
    CTAAAAGAGG TTCTTTCTGA TCTCAAAGAA CAGATTTTTG ATCTAGAATT TAACAGACTT
    GGTCAACTAA AGGAAGAAAT GAGGGAAGTC CAGCACCAGA GAGCACAAAT AGAAAGCATC
    CAATCCCAGG TGAAGCATGA GAGGCGAGAA GTTCTGGCTA TGCAGTTCCA AGAATGGACT
    TTGGATGTCA GTGGAAGGAT CCCTCTTGTG CTGGTACAAA GTGCATTAAA ATCTTTTTCG
    GAAGTAATAG CTAACCTTCC ATTGGATGAA AAAGCAGCTT ATGAAGAGGA GCTGAAGACG
    GTGAACACAA TGGAATGTAT GCTAACTCAG GAAGAATTTG TGGAGTATGT GCATCCATTT
    ATCTGTCACT TCAGTACAGA CTTATTTCAG GAGTTCCTGC ATCATCTCTC ACAGTGTGCA
    GATACATTTC GTGAAACCAT TCGACATGAC ATCTGGAGAC AGATGTTTTC TGATCTCTTC
    TTTGCATGTG ACCCAGGAAA GGTTGGCTTT CTTGACAGAC AGAGAGTCCT GGCTTTACTG
    GAAACTTTCT ATGAAAAAAC TATTTCAGAA ACTGATGAAT GGCAGCCACG AAACCCTAGG
    CAATGGCCAG TGGTTGAGCT GGATGAAATG GATCCAACAG ACTTTTGGGC TGACTTCCAA
    GAAGGAGTAA AGTCTTTAGA GGAACAAGAA GGTTCCGTTA TGGCAATCTC AGCAACGACT
    CAAATATCAA CTCCAACAGA AGGCGATTAT CATTTAATAG GCCCTATGCA AGAACAAGAT
    AAGCTAGATG GAATTCCTTC AACTGAAGGC AGTAGTGAAT CACATCTTAT TGGTTTGCCA
    TTCCCAACAA AGTCAGAGGC ACCTACTGCT GAAGACATTA GGAGTAGTGA CTCATATCTC
    ATTGATGGAA AACCTTGGTC AGGAAAACTT CTGACTGCTG ATCTGGCCAT TAAGTACAGC
    TCTTACGGTG ACAAAATCCA AGATGAGTGC AATGCAGCAG CTTCTAAATT CACAGATTTG
    CACCCTATCA TGTCAGAGAT TAACTCCCGA GGCCCATCAT GCATTGCAAG TGCGTTCAAT
    GACAGTGCAT TAAATTTACC CCAGTTTGTA CAATTGATGG AGACTTTCCT GGGGGAAGGG
    GCACCACACT CATTTGTGGA GAAGCTTGTA TCCTTTATAC AGAAGGAATA TGCTGAGACA
    GAAGAGGAGA AGGTGGCATT GTTGTCAAAA GTTCGACAAG ATGCCTTACT GGCACGACAA
    AAGTTGGTGC TGCAAGCTCT CTTTGTGAAG TGGGACAATG ATGGATCTGG GTTTCTGGGA
    CTAGATGAGG TTGAGGCTCT GCTGTTCAAG TACAAGGAAG GCATGGAAAC TGAGGTGATG
    GCAAGAGGAA GGGAAGCACT GAGTTCCTCC CAATCTCAAA GGAGCATTCA GAGGCTCTCG
    GCTCCTGAAT TCTGCAAATA CATTCAGGGT GTGGTGAGGC AGCTGCCTGG TCCTGATGAG
    GAGGTGTTTG AAAGCTTGGT GGAATACCTG ACATCCAGTG TGGAACGTTC TCAGGCTGAG
    AAGCTGCGAG GGGCTTCCAG AAGAAAGTGG TTGCAGCAAA TCCAAGTGGC AGCTGAGACA
    AGTGGAGGAA ATCTGCAGCC TGTGTACAAA GCTCTGTTTC AGGCACTTTT TAAGGATGCA
    GAGGCCCATG GAAATAACAA AAGGATTAGT GCTAACATTG GCCTTCTGGA GGAGAGCTCA
    GTTGGTGGAG GATGGACACA GATCATCCGT TATGTAGCTT GTACTGCAGA TGATACTCCG
    TATGTACTAA ACAAGACCCT ACAAAGAGAT ATGAAGGGTG TGAGCTTTGC AGCAGTGGAG
    AGCGGAATAC CACAGCATGT GCCTCGGGTT CAGTACCACG GACACATCCA TTTCTGGAAT
    ACAGACCGAC CAGAAGAAGA GCGCAAAGGG TCTCTTATTG TCCTGCCTCT CATTGATATG
    CAACAACGGG CATTTGGTAC CCTTTCCATT GATACAATGA GAGATCCCCG GGAAAGGAAT
    ATATTTCTCA CTCATGAGAT CAGCTTTTAT CAGGGGGTCA CCAATGCATT CAGTGCATCA
    TATAATCACG TGCAACAAAT CAAGGATATT CTTCAACTAA TAAGCAGTGC CCTTGCCTGG
    ATCTACAGCC GTGCTCCCAA CATCCGCAGC ATCACCACAT ACCTAATGGA GCCCAGTGTA
    GATAAGGGTT ATGTGTTTCG CAAAATGATG AGCACAGATA ATAACACAGG GCTGTCTGAA
    ATCTACAGCT CCCCAGCCAT GCTGCGCAGG CAGGATAATC TTTTCAGGGA TTACCTCTTT
    AAGTGTGCCG ATAGCTCAGA AGTTATTACA ACAGATGCCT ATGGAGAGCG TCACATTGCT
    GTACCTATTC GCAACTCAGC GGGACGGGCA CTTGGAGTGC TGGATCTAAG CACTGGGCAG
    TGCAAGGAGC TACCTGCACA CGAGTATCAA GATCTGCAAA AAATGCTGCA AATGCTGCAG
    GAAGCTTGCT ATGAAATCCT AGAGGAGAAC CAATTCAGCG ACACCCAGAA AAAGTCTATC
    CTAGAGGCAG AGCGAGTGTC TGGGCACAGA CGGGCAGCGG TTCTGTTCCA TCGCTTCATG
    CTGCAGGATC TGCGTCAGTG TGTGAGCAAA CTCGACCACC AGTCTTTTGC TGAGCTCAAG
    AGTTACAAGG AACCCCCTAC CATGGTGCAT AGTATCCTCA AAGCTGTGCT GCTCCTCTTC
    TGCCCTGACT GGGCCGAATC AGATGAAGTT GAAAGCTGGA ATCAGTGTAA ACTGAAAGTT
    AACAGCGATT TGATCAGGAA AATTTTATTC TTTGACCCAA CTGCCCAATC TGTGCAGATT
    AAACCAGAGA TGCTGGCCAA GTATATCAAA GGTATTCCCA GGGGTGCAGT TTGGAAACAT
    GGCTCCATCC CTGCTGAACA TCTATATAAC TGGGCCTTCA CCTGTCTCTC CCTAATGGAA
    CTTACAGAGA AAATCCCAAA TACACAGAGT CCATCACTGA TCACTGTGCC AGTACCAAAC
    AGAAGTGAAA ACGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031752526.1
    CDS190..3747
    Translation

    Target ORF information:

    RefSeq Version XM_031896666.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis EF-hand calcium binding domain 5 (efcab5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896666.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGATGCAG AAAGTGATTA CAGTGCTCAC ACCTCCTCCC TGGACTGTAG TAGTGAAAAA 
    CTAGAAAGCA ACCCAAAAAA CAATGCCATT CAGACTCAAG ACAGAGGTGG TGACTGGAGA
    ACAATATTTT TTGACAAAAT GCAAGAAAGA GGATTAAAGC TACAACAGAA GACCTTGGAA
    AGTCTAAAAC TCCAGCAAGC ACAAGAAAAG AAAGAAAAAA AGGCAAATCC TCCAGATGAA
    CAGGCCAAAG ATTGGTTTAG CCAGGATAAA ACAACTGTGC ATACCAGAGC CTACTTACTT
    GACAAGCTGA TGCCTTCCAT TGTACCGGGG CTGGAAGCTC TACTTAAAGA ATTAGAGAAA
    AGAAAGGTTC TAGAAGACAG TTCCATTGCA TTTGATCCAA TTATATATCT TGGAGAGCAG
    CTCATGAGGC ATAATCCAGA GTATGTTAGG GATCTTCAAA GCAATTCATA TGTACAAGGG
    CTAAAAGAGG TTCTTTCTGA TCTCAAAGAA CAGATTTTTG ATCTAGAATT TAACAGACTT
    GGTCAACTAA AGGAAGAAAT GAGGGAAGTC CAGCACCAGA GAGCACAAAT AGAAAGCATC
    CAATCCCAGG TGAAGCATGA GAGGCGAGAA GTTCTGGCTA TGCAGTTCCA AGAATGGACT
    TTGGATGTCA GTGGAAGGAT CCCTCTTGTG CTGGTACAAA GTGCATTAAA ATCTTTTTCG
    GAAGTAATAG CTAACCTTCC ATTGGATGAA AAAGCAGCTT ATGAAGAGGA GCTGAAGACG
    GTGAACACAA TGGAATGTAT GCTAACTCAG GAAGAATTTG TGGAGTATGT GCATCCATTT
    ATCTGTCACT TCAGTACAGA CTTATTTCAG GAGTTCCTGC ATCATCTCTC ACAGTGTGCA
    GATACATTTC GTGAAACCAT TCGACATGAC ATCTGGAGAC AGATGTTTTC TGATCTCTTC
    TTTGCATGTG ACCCAGGAAA GGTTGGCTTT CTTGACAGAC AGAGAGTCCT GGCTTTACTG
    GAAACTTTCT ATGAAAAAAC TATTTCAGAA ACTGATGAAT GGCAGCCACG AAACCCTAGG
    CAATGGCCAG TGGTTGAGCT GGATGAAATG GATCCAACAG ACTTTTGGGC TGACTTCCAA
    GAAGGAGTAA AGTCTTTAGA GGAACAAGAA GGTTCCGTTA TGGCAATCTC AGCAACGACT
    CAAATATCAA CTCCAACAGA AGGCGATTAT CATTTAATAG GCCCTATGCA AGAACAAGAT
    AAGCTAGATG GAATTCCTTC AACTGAAGGC AGTAGTGAAT CACATCTTAT TGGTTTGCCA
    TTCCCAACAA AGTCAGAGGC ACCTACTGCT GAAGACATTA GGAGTAGTGA CTCATATCTC
    ATTGATGGAA AACCTTGGTC AGGAAAACTT CTGACTGCTG ATCTGGCCAT TAAGTACAGC
    TCTTACGGTG ACAAAATCCA AGATGAGTGC AATGCAGCAG CTTCTAAATT CACAGATTTG
    CACCCTATCA TGTCAGAGAT TAACTCCCGA GGCCCATCAT GCATTGCAAG TGCGTTCAAT
    GACAGTGCAT TAAATTTACC CCAGTTTGTA CAATTGATGG AGACTTTCCT GGGGGAAGGG
    GCACCACACT CATTTGTGGA GAAGCTTGTA TCCTTTATAC AGAAGGAATA TGCTGAGACA
    GAAGAGGAGA AGGTGGCATT GTTGTCAAAA GTTCGACAAG ATGCCTTACT GGCACGACAA
    AAGTTGGTGC TGCAAGCTCT CTTTGTGAAG TGGGACAATG ATGGATCTGG GTTTCTGGGA
    CTAGATGAGG TTGAGGCTCT GCTGTTCAAG TACAAGGAAG GCATGGAAAC TGAGGTGATG
    GCAAGAGGAA GGGAAGCACT GAGTTCCTCC CAATCTCAAA GGAGCATTCA GAGGCTCTCG
    GCTCCTGAAT TCTGCAAATA CATTCAGGGT GTGGTGAGGC AGCTGCCTGG TCCTGATGAG
    GAGGTGTTTG AAAGCTTGGT GGAATACCTG ACATCCAGTG TGGAACGTTC TCAGGCTGAG
    AAGCTGCGAG GGGCTTCCAG AAGAAAGTGG TTGCAGCAAA TCCAAGTGGC AGCTGAGACA
    AGTGGAGGAA ATCTGCAGCC TGTGTACAAA GCTCTGTTTC AGGCACTTTT TAAGGATGCA
    GAGGCCCATG GAAATAACAA AAGGATTAGT GCTAACATTG GCCTTCTGGA GGAGAGCTCA
    GTTGGTGGAG GATGGACACA GATCATCCGT TATGTAGCTT GTACTGCAGA TGATACTCCG
    TATGTACTAA ACAAGACCCT ACAAAGAGAT ATGAAGGGTG TGAGCTTTGC AGCAGTGGAG
    AGCGGAATAC CACAGCATGT GCCTCGGGTT CAGTACCACG GACACATCCA TTTCTGGAAT
    ACAGACCGAC CAGAAGAAGA GCGCAAAGGG TCTCTTATTG TCCTGCCTCT CATTGATATG
    CAACAACGGG CATTTGGTAC CCTTTCCATT GATACAATGA GAGATCCCCG GGAAAGGAAT
    ATATTTCTCA CTCATGAGAT CAGCTTTTAT CAGGGGGTCA CCAATGCATT CAGTGCATCA
    TATAATCACG TGCAACAAAT CAAGGATATT CTTCAACTAA TAAGCAGTGC CCTTGCCTGG
    ATCTACAGCC GTGCTCCCAA CATCCGCAGC ATCACCACAT ACCTAATGGA GCCCAGTGTA
    GATAAGGGTT ATGTGTTTCG CAAAATGATG AGCACAGATA ATAACACAGG GCTGTCTGAA
    ATCTACAGCT CCCCAGCCAT GCTGCGCAGG CAGGATAATC TTTTCAGGGA TTACCTCTTT
    AAGTGTGCCG ATAGCTCAGA AGTTATTACA ACAGATGCCT ATGGAGAGCG TCACATTGCT
    GTACCTATTC GCAACTCAGC GGGACGGGCA CTTGGAGTGC TGGATCTAAG CACTGGGCAG
    TGCAAGGAGC TACCTGCACA CGAGTATCAA GATCTGCAAA AAATGCTGCA AATGCTGCAG
    GAAGCTTGCT ATGAAATCCT AGAGGAGAAC CAATTCAGCG ACACCCAGAA AAAGTCTATC
    CTAGAGGCAG AGCGAGTGTC TGGGCACAGA CGGGCAGCGG TTCTGTTCCA TCGCTTCATG
    CTGCAGGATC TGCGTCAGTG TGTGAGCAAA CTCGACCACC AGTCTTTTGC TGAGCTCAAG
    AGTTACAAGG AACCCCCTAC CATGGTGCAT AGTATCCTCA AAGCTGTGCT GCTCCTCTTC
    TGCCCTGACT GGGCCGAATC AGATGAAGTT GAAAGCTGGA ATCAGTGTAA ACTGAAAGTT
    AACAGCGATT TGATCAGGAA AATTTTATTC TTTGACCCAA CTGCCCAATC TGTGCAGATT
    AAACCAGAGA TGCTGGCCAA GTATATCAAA GGTATTCCCA GGGGTGCAGT TTGGAAACAT
    GGCTCCATCC CTGCTGAACA TCTATATAAC TGGGCCTTCA CCTGTCTCTC CCTAATGGAA
    CTTACAGAGA AAATCCCAAA TACACAGAGT CCATCACTGA TCACTGTGCC AGTACCAAAC
    AGAAGTGAAA ACGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.