edem3 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following edem3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the edem3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa09193 XM_002932144.4
Latest version!
Xenopus tropicalis ER degradation enhancer, mannosidase alpha-like 3 (edem3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa44212 XM_018093640.1
Latest version!
Xenopus tropicalis ER degradation enhancer, mannosidase alpha-like 3 (edem3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OXa57355 XM_018093640.2
Latest version!
Xenopus tropicalis ER degradation enhancer, mannosidase alpha-like 3 (edem3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa57356 XM_002932144.5
Latest version!
Xenopus tropicalis ER degradation enhancer, mannosidase alpha-like 3 (edem3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa09193
    Clone ID Related Accession (Same CDS sequence) XM_002932144.4
    Accession Version XM_002932144.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2742bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002932144.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGGCTGCC CAGTAATAGA GCCCCGCCGC TGGGGTGACA TGTGGCTAGT GCTGGCCTTC 
    TGTCTGCTGG GACAGGGACG CGCTGCTGTG ACCAAGGAGG AGAAGGCTCA GTTAAGGAGT
    CAAGTTCTGG AGATGTTTGA TCATGCATAT GGAAGTTATA TGCAACATGC TTATCCAGCT
    GATGAACTCA TGCCCTTAAC CTGCCGTGGA AGAATACGTG GCCAAGAACC AAGCAGAGGG
    GATGTTGATG ATGCTTTGGG AAAATTTTCA CTAACATTAA TCGACACACT GGACACACTT
    GTGGTGTTAA ACAAAACCAA TGAGTTTGAA GATGCAGTCC GAAAAGTGAT TACTGATGTC
    AATTTAGACA ATGATATAGT GGTGTCTGTC TTTGAGACAA ATATCAGAGT CTTGGGTGGC
    CTCCTAGGCG GACACTCACT TGCCATCATG CTGAAAGAAA ACAGCGATCG AATGCAGTGG
    TACAATGATG AACTCTTGCA CATGGCTAAG GAGCTTGGCT ATAAGCTCCT TCCTGCGTTT
    AACACCACCA GTGGCCTTCC CTACCCCAGA ATTAATTTGA AATTTGGCAT CAGAAGTCCA
    GAGGCTCGGA CGGGGACAGA GACCGATACA TGCACAGCTT GTGCAGGGAC AATGATCTTG
    GAATTCGCTG CACTGAGCCG CTTTACTGGA ATAGCCGTAT TTGAGGAACA TGCCAGGAAA
    GCCCTCGATT TTCTCTGGGA GAAAAGGCAG CGCAGTAGCA ACTTGGTCGG TGTGACCATT
    AATATCCATA CAGGGGACTG GGTACGCAAA GACAGTGGTG TGGGAGCTGG AATAGATTCT
    TACTATGAAT ATTTACTGAA GGCCTACGTT CTCCTGGGTG ATGACAGCTA TCTTGAAAGA
    TTTAATACAC ACTATGATGC CATTATGCGA TATATAAGCC AGCCTCCGCT TCTGCTTGAT
    GTACATATCC ACAAACCAAT GCTGACAGCA CGAACATGGA TGGATTCACT GCTTGCATTT
    TTTCCTGGTC TCCAGGTTTT GAAGGGGGAC ATCAGACCTG CAATTGAGAC TCATGAAATG
    CTTTACCAGG TGATAAAAAA GCACAATTTC CTGCCAGAGG CCTTTACAAC GGATTTCAGG
    GTTCACTGGG CACAGCACCC TTTAAGACCA GAATTTGCTG AAAGTACTTA TTTCCTTTAT
    AAAGCCACTG GTGATCCTTA CTACCTCGAA GTGGGAAGAA CTCTAATAGA TAATCTGAAT
    AAATATGCAC GGGTGCCATG TGGATTTGCT GCTGTTAAAG ATGTGAGAAC TGGTAGCCAT
    GAAGACCGAA TGGACTCCTT TTTCCTGGCA GAAATGTTTA AGTACCTCTA TTTGCTCTTC
    TCAGAAAGGG AAGACCTTAT ATTTGATATA GAAAGTTATA TATTTACTAC AGAAGCCCAT
    CTCCTACCTC TCTCTCTTTC CACTGCAAGT CAGACCAGCA CAAAAAAAAA CACAACTTCT
    CAATACACCG AACTAGATGA TAGTAACTTT GATTGGTCTT GCCCAAACAC CCAGATCCTC
    TTCCGAAATG ACCCAATGTA TGCACAGAAT ATTCGGGAAC CTGTAAAAAA TGTTGTTGAT
    AAGAATTGCC CCCGGAGTCC TTCTCGTCTA GATGAGGTTT CTGGCAGTGG AAATAAGCCA
    CCGCTGCGGG CTCGAGATTT CATGGCCACT AATTCAGAAC ATTTAGAGAT TCTGAAGAAG
    ATGGGCGTGA GTCTGATTCA CCTCAAGGAT GGCCGTGTTC AGTTGGTCCA GCATGCCAAC
    CAAGCTGCAA GCTCAATTGA TGCTGAGGAT GGGCTTCGAT TTATGCAAGA AATGATTGAG
    CTTTCTAGCC AGCAGCAGAA AGAGCAGCAG CTGCCTCCCC GTGCCGTACA GATTGTGTCG
    CACCCATTTT ATGGGAGAGT TGTACTAACT GCGGGACCAG CTCAGTTTGG AATGGACCTT
    TCTAAACATT TAGCAGGGGC ACGAGGCTTG GTAGCAAGAG CTGAGCCATA CAGCGGGTGC
    TCTGATATCA CCAATGGAGC AGCAATACAA GGCAAGATTG CACTGATGCA GAGAGGACAA
    TGTATGTTTG CTGAGAAGGC TCGCAATGTT CAGAAAGCTG GGGCCATTGG AGGTATTGTT
    ATAGATGACA ACGAAGGTAG TAGCAGTGAC ACTGCACCTC TCTTCCAAAT GGCTGGAGAT
    GGGAAGAATA CGGATGACAT TACCATCCCC ATGTTATTTC TCTTCAGCAA GGAAGGAAAT
    ATCATTCTTG ATGCAATTCG TGAATACCAG CAGGTGGAGG TTCTACTCTC CGATAAAGCC
    AAAGACCGAG ATTTAGAAAA CGAAGGTGGA GAACAGAAGC CAGTAGAAAA TGATTCCCAG
    AAACAGGCTT TAGACGATAT GTTTATGACC CCAGAAGAAA TTGAAGAGCT TTCTCTAGTG
    CCTCTGGAAG AATTGCCTGT TTCTCAGCTA GAGGTGCCAA GTTCTGACTC TCCATCTGGT
    GGGGGCAGCA CTTTAGAAAG TGACATTACA GCAGAAAGTA AAGACCATAA GACTGAAGAA
    ACTGCACACA GCTCTAAAGA CAATTTCCAG ACCCCACCAG AAACCTCTGA AGACTCCACA
    GAGGAAAAAT GGGACAACAA AGTTCGGCCA ATGGAGTCAA TATTGGCAGA CTGGAAAGAG
    GACATTGAGG CCTTTGAAAT GATGGAGAAG GATGAACTAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002932190.2
    CDS276..3017
    Translation

    Target ORF information:

    RefSeq Version XM_002932144.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ER degradation enhancer, mannosidase alpha-like 3 (edem3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002932144.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGGCTGCC CAGTAATAGA GCCCCGCCGC TGGGGTGACA TGTGGCTAGT GCTGGCCTTC 
    TGTCTGCTGG GACAGGGACG CGCTGCTGTG ACCAAGGAGG AGAAGGCTCA GTTAAGGAGT
    CAAGTTCTGG AGATGTTTGA TCATGCATAT GGAAGTTATA TGCAACATGC TTATCCAGCT
    GATGAACTCA TGCCCTTAAC CTGCCGTGGA AGAATACGTG GCCAAGAACC AAGCAGAGGG
    GATGTTGATG ATGCTTTGGG AAAATTTTCA CTAACATTAA TCGACACACT GGACACACTT
    GTGGTGTTAA ACAAAACCAA TGAGTTTGAA GATGCAGTCC GAAAAGTGAT TACTGATGTC
    AATTTAGACA ATGATATAGT GGTGTCTGTC TTTGAGACAA ATATCAGAGT CTTGGGTGGC
    CTCCTAGGCG GACACTCACT TGCCATCATG CTGAAAGAAA ACAGCGATCG AATGCAGTGG
    TACAATGATG AACTCTTGCA CATGGCTAAG GAGCTTGGCT ATAAGCTCCT TCCTGCGTTT
    AACACCACCA GTGGCCTTCC CTACCCCAGA ATTAATTTGA AATTTGGCAT CAGAAGTCCA
    GAGGCTCGGA CGGGGACAGA GACCGATACA TGCACAGCTT GTGCAGGGAC AATGATCTTG
    GAATTCGCTG CACTGAGCCG CTTTACTGGA ATAGCCGTAT TTGAGGAACA TGCCAGGAAA
    GCCCTCGATT TTCTCTGGGA GAAAAGGCAG CGCAGTAGCA ACTTGGTCGG TGTGACCATT
    AATATCCATA CAGGGGACTG GGTACGCAAA GACAGTGGTG TGGGAGCTGG AATAGATTCT
    TACTATGAAT ATTTACTGAA GGCCTACGTT CTCCTGGGTG ATGACAGCTA TCTTGAAAGA
    TTTAATACAC ACTATGATGC CATTATGCGA TATATAAGCC AGCCTCCGCT TCTGCTTGAT
    GTACATATCC ACAAACCAAT GCTGACAGCA CGAACATGGA TGGATTCACT GCTTGCATTT
    TTTCCTGGTC TCCAGGTTTT GAAGGGGGAC ATCAGACCTG CAATTGAGAC TCATGAAATG
    CTTTACCAGG TGATAAAAAA GCACAATTTC CTGCCAGAGG CCTTTACAAC GGATTTCAGG
    GTTCACTGGG CACAGCACCC TTTAAGACCA GAATTTGCTG AAAGTACTTA TTTCCTTTAT
    AAAGCCACTG GTGATCCTTA CTACCTCGAA GTGGGAAGAA CTCTAATAGA TAATCTGAAT
    AAATATGCAC GGGTGCCATG TGGATTTGCT GCTGTTAAAG ATGTGAGAAC TGGTAGCCAT
    GAAGACCGAA TGGACTCCTT TTTCCTGGCA GAAATGTTTA AGTACCTCTA TTTGCTCTTC
    TCAGAAAGGG AAGACCTTAT ATTTGATATA GAAAGTTATA TATTTACTAC AGAAGCCCAT
    CTCCTACCTC TCTCTCTTTC CACTGCAAGT CAGACCAGCA CAAAAAAAAA CACAACTTCT
    CAATACACCG AACTAGATGA TAGTAACTTT GATTGGTCTT GCCCAAACAC CCAGATCCTC
    TTCCGAAATG ACCCAATGTA TGCACAGAAT ATTCGGGAAC CTGTAAAAAA TGTTGTTGAT
    AAGAATTGCC CCCGGAGTCC TTCTCGTCTA GATGAGGTTT CTGGCAGTGG AAATAAGCCA
    CCGCTGCGGG CTCGAGATTT CATGGCCACT AATTCAGAAC ATTTAGAGAT TCTGAAGAAG
    ATGGGCGTGA GTCTGATTCA CCTCAAGGAT GGCCGTGTTC AGTTGGTCCA GCATGCCAAC
    CAAGCTGCAA GCTCAATTGA TGCTGAGGAT GGGCTTCGAT TTATGCAAGA AATGATTGAG
    CTTTCTAGCC AGCAGCAGAA AGAGCAGCAG CTGCCTCCCC GTGCCGTACA GATTGTGTCG
    CACCCATTTT ATGGGAGAGT TGTACTAACT GCGGGACCAG CTCAGTTTGG AATGGACCTT
    TCTAAACATT TAGCAGGGGC ACGAGGCTTG GTAGCAAGAG CTGAGCCATA CAGCGGGTGC
    TCTGATATCA CCAATGGAGC AGCAATACAA GGCAAGATTG CACTGATGCA GAGAGGACAA
    TGTATGTTTG CTGAGAAGGC TCGCAATGTT CAGAAAGCTG GGGCCATTGG AGGTATTGTT
    ATAGATGACA ACGAAGGTAG TAGCAGTGAC ACTGCACCTC TCTTCCAAAT GGCTGGAGAT
    GGGAAGAATA CGGATGACAT TACCATCCCC ATGTTATTTC TCTTCAGCAA GGAAGGAAAT
    ATCATTCTTG ATGCAATTCG TGAATACCAG CAGGTGGAGG TTCTACTCTC CGATAAAGCC
    AAAGACCGAG ATTTAGAAAA CGAAGGTGGA GAACAGAAGC CAGTAGAAAA TGATTCCCAG
    AAACAGGCTT TAGACGATAT GTTTATGACC CCAGAAGAAA TTGAAGAGCT TTCTCTAGTG
    CCTCTGGAAG AATTGCCTGT TTCTCAGCTA GAGGTGCCAA GTTCTGACTC TCCATCTGGT
    GGGGGCAGCA CTTTAGAAAG TGACATTACA GCAGAAAGTA AAGACCATAA GACTGAAGAA
    ACTGCACACA GCTCTAAAGA CAATTTCCAG ACCCCACCAG AAACCTCTGA AGACTCCACA
    GAGGAAAAAT GGGACAACAA AGTTCGGCCA ATGGAGTCAA TATTGGCAGA CTGGAAAGAG
    GACATTGAGG CCTTTGAAAT GATGGAGAAG GATGAACTAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa44212
    Clone ID Related Accession (Same CDS sequence) XM_018093640.1
    Accession Version XM_018093640.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2757bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGGCTGCC CAGTAATAGA GCCCCGCCGC TGGGGTGACA TGTGGCTAGT GCTGGCCTTC 
    TGTCTGCTGG GACAGGGACG CGCTGCTGTG ACCAAGGAGG AGAAGGCTCA GTTAAGGAGT
    CAAGTTCTGG AGATGTTTGA TCATGCATAT GGAAGTTATA TGCAACATGC TTATCCAGCT
    GATGAACTCA TGCCCTTAAC CTGCCGTGGA AGAATACGTG GCCAAGAACC AAGCAGAGGG
    GATGTTGATG ATGCTTTGGG AAAATTTTCA CTAACATTAA TCGACACACT GGACACACTT
    GTGGTGTTAA ACAAAACCAA TGAGTTTGAA GATGCAGTCC GAAAAGTGAT TACTGATGTC
    AATTTAGACA ATGATATAGT GGTGTCTGTC TTTGAGACAA ATATCAGAGT CTTGGGTGGC
    CTCCTAGGCG GACACTCACT TGCCATCATG CTGAAAGAAA ACAGCGATCG AATGCAGTGG
    TACAATGATG AACTCTTGCA CATGGCTAAG GAGCTTGGCT ATAAGCTCCT TCCTGCGTTT
    AACACCACCA GTGGCCTTCC CTACCCCAGA ATTAATTTGA AATTTGGCAT CAGAAGTCCA
    GAGGCTCGGA CGGGGACAGA GACCGATACA TGCACAGCTT GTGCAGGGAC AATGATCTTG
    GAATTCGCTG CACTGAGCCG CTTTACTGGA ATAGCCGTAT TTGAGGAACA TGCCAGGAAA
    GCCCTCGATT TTCTCTGGGA GAAAAGGCAG CGCAGTAGCA ACTTGGTCGG TGTGACCATT
    AATATCCATA CAGGGGACTG GGTACGCAAA GACAGTGGTG TGGGAGCTGG AATAGATTCT
    TACTATGAAT ATTTACTGAA GGCCTACGTT CTCCTGGGTG ATGACAGCTA TCTTGAAAGA
    TTTAATACAC ACTATGATGC CATTATGCGA TATATAAGCC AGCCTCCGCT TCTGCTTGAT
    GTACATATCC ACAAACCAAT GCTGACAGCA CGAACATGGA TGGATTCACT GCTTGCATTT
    TTTCCTGGTC TCCAGGTTTT GAAGGGGGAC ATCAGACCTG CAATTGAGAC TCATGAAATG
    CTTTACCAGG TGATAAAAAA GCACAATTTC CTGCCAGAGG CCTTTACAAC GGATTTCAGG
    GTTCACTGGG CACAGCACCC TTTAAGACCA GAATTTGCTG AAAGTACTTA TTTCCTTTAT
    AAAGCCACTG GTGATCCTTA CTACCTCGAA GTGGGAAGAA CTCTAATAGA TAATCTGAAT
    AAATATGCAC GGGTGCCATG TGGATTTGCT GCTGTTAAAG ATGTGAGAAC TGGTAGCCAT
    GAAGACCGAA TGGACTCCTT TTTCCTGGCA GAAATGTTTA AGTACCTCTA TTTGCTCTTC
    TCAGAAAGGG AAGACCTTAT ATTTGATATA GAAAGTTATA TATTTACTAC AGAAGCCCAT
    CTCCTACCTC TCTCTCTTTC CACTGCAAGT CAGACCAGCA CAAAAAAAAA CACAACTTCT
    CAATACACCG AACTAGATGA TAGTAACTTT GATTGGTCTT GCCCAAACAC CCAGATCCTC
    TTCCGAAATG ACCCAATGTA TGCACAGAAT ATTCGGGAAC CTGTAAAAAA TGTTGTTGAT
    AAGAATTGCC CCCGGAGTCC TTCTCGTCTA GATGAGGTTT CTGGCAGTGG AAATAAGCCA
    CCGCTGCGGG CTCGAGATTT CATGGCCACT AATTCAGAAC ATTTAGAGAT TCTGAAGAAG
    ATGGGCGTGA GTCTGATTCA CCTCAAGGAT GGCCGTGTTC AGTTGGTCCA GCATGCCAAC
    CAAGCTGCAA GCTCAATTGA TGCTGAGGAT GGGCTTCGAT TTATGCAAGA AATGATTGAG
    CTTTCTAGCC AGCAGCAGAA AGAGCAGCAG CTGCCTCCCC GTGCCGTACA GATTGTGTCG
    CACCCATTTT ATGGGAGAGT TGTACTAACT GCGGGACCAG CTCAGTTTGG AATGGACCTT
    TCTAAACATT TAGCAGGGGC ACGAGGCTTG GTAGCAAGAG CTGAGCCATA CAGCGGGTGC
    TCTGATATCA CCAATGGAGC AGCAATACAA GGCAAGATTG CACTGATGCA GAGAGGACAA
    TGTATGTTTG CTGAGAAGGC TCGCAATGTT CAGAAAGCTG GGGCCATTGG AGGTATTGTT
    ATAGATGACA ACGAAGGTAG TAGCAGTGAC ACTGCACCTC TCTTCCAAAT GGCTGGAGAT
    GGGAAGAATA CGGATGACAT TACCATCCCC ATGTTATTTC TCTTCAGCAA GGAAGGAAAT
    ATCATTCTTG ATGCAATTCG TGAATACCAG CAGGTGGAGG TTCTACTCTC CGATAAAGCC
    AAAGACCGAG CAACCATTTT TAAAGATTTA GAAAACGAAG GTGGAGAACA GAAGCCAGTA
    GAAAATGATT CCCAGAAACA GGCTTTAGAC GATATGTTTA TGACCCCAGA AGAAATTGAA
    GAGCTTTCTC TAGTGCCTCT GGAAGAATTG CCTGTTTCTC AGCTAGAGGT GCCAAGTTCT
    GACTCTCCAT CTGGTGGGGG CAGCACTTTA GAAAGTGACA TTACAGCAGA AAGTAAAGAC
    CATAAGACTG AAGAAACTGC ACACAGCTCT AAAGACAATT TCCAGACCCC ACCAGAAACC
    TCTGAAGACT CCACAGAGGA AAAATGGGAC AACAAAGTTC GGCCAATGGA GTCAATATTG
    GCAGACTGGA AAGAGGACAT TGAGGCCTTT GAAATGATGG AGAAGGATGA ACTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017949129.1
    CDS277..3033
    Translation

    Target ORF information:

    RefSeq Version XM_018093640.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ER degradation enhancer, mannosidase alpha-like 3 (edem3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018093640.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGGCTGCC CAGTAATAGA GCCCCGCCGC TGGGGTGACA TGTGGCTAGT GCTGGCCTTC 
    TGTCTGCTGG GACAGGGACG CGCTGCTGTG ACCAAGGAGG AGAAGGCTCA GTTAAGGAGT
    CAAGTTCTGG AGATGTTTGA TCATGCATAT GGAAGTTATA TGCAACATGC TTATCCAGCT
    GATGAACTCA TGCCCTTAAC CTGCCGTGGA AGAATACGTG GCCAAGAACC AAGCAGAGGG
    GATGTTGATG ATGCTTTGGG AAAATTTTCA CTAACATTAA TCGACACACT GGACACACTT
    GTGGTGTTAA ACAAAACCAA TGAGTTTGAA GATGCAGTCC GAAAAGTGAT TACTGATGTC
    AATTTAGACA ATGATATAGT GGTGTCTGTC TTTGAGACAA ATATCAGAGT CTTGGGTGGC
    CTCCTAGGCG GACACTCACT TGCCATCATG CTGAAAGAAA ACAGCGATCG AATGCAGTGG
    TACAATGATG AACTCTTGCA CATGGCTAAG GAGCTTGGCT ATAAGCTCCT TCCTGCGTTT
    AACACCACCA GTGGCCTTCC CTACCCCAGA ATTAATTTGA AATTTGGCAT CAGAAGTCCA
    GAGGCTCGGA CGGGGACAGA GACCGATACA TGCACAGCTT GTGCAGGGAC AATGATCTTG
    GAATTCGCTG CACTGAGCCG CTTTACTGGA ATAGCCGTAT TTGAGGAACA TGCCAGGAAA
    GCCCTCGATT TTCTCTGGGA GAAAAGGCAG CGCAGTAGCA ACTTGGTCGG TGTGACCATT
    AATATCCATA CAGGGGACTG GGTACGCAAA GACAGTGGTG TGGGAGCTGG AATAGATTCT
    TACTATGAAT ATTTACTGAA GGCCTACGTT CTCCTGGGTG ATGACAGCTA TCTTGAAAGA
    TTTAATACAC ACTATGATGC CATTATGCGA TATATAAGCC AGCCTCCGCT TCTGCTTGAT
    GTACATATCC ACAAACCAAT GCTGACAGCA CGAACATGGA TGGATTCACT GCTTGCATTT
    TTTCCTGGTC TCCAGGTTTT GAAGGGGGAC ATCAGACCTG CAATTGAGAC TCATGAAATG
    CTTTACCAGG TGATAAAAAA GCACAATTTC CTGCCAGAGG CCTTTACAAC GGATTTCAGG
    GTTCACTGGG CACAGCACCC TTTAAGACCA GAATTTGCTG AAAGTACTTA TTTCCTTTAT
    AAAGCCACTG GTGATCCTTA CTACCTCGAA GTGGGAAGAA CTCTAATAGA TAATCTGAAT
    AAATATGCAC GGGTGCCATG TGGATTTGCT GCTGTTAAAG ATGTGAGAAC TGGTAGCCAT
    GAAGACCGAA TGGACTCCTT TTTCCTGGCA GAAATGTTTA AGTACCTCTA TTTGCTCTTC
    TCAGAAAGGG AAGACCTTAT ATTTGATATA GAAAGTTATA TATTTACTAC AGAAGCCCAT
    CTCCTACCTC TCTCTCTTTC CACTGCAAGT CAGACCAGCA CAAAAAAAAA CACAACTTCT
    CAATACACCG AACTAGATGA TAGTAACTTT GATTGGTCTT GCCCAAACAC CCAGATCCTC
    TTCCGAAATG ACCCAATGTA TGCACAGAAT ATTCGGGAAC CTGTAAAAAA TGTTGTTGAT
    AAGAATTGCC CCCGGAGTCC TTCTCGTCTA GATGAGGTTT CTGGCAGTGG AAATAAGCCA
    CCGCTGCGGG CTCGAGATTT CATGGCCACT AATTCAGAAC ATTTAGAGAT TCTGAAGAAG
    ATGGGCGTGA GTCTGATTCA CCTCAAGGAT GGCCGTGTTC AGTTGGTCCA GCATGCCAAC
    CAAGCTGCAA GCTCAATTGA TGCTGAGGAT GGGCTTCGAT TTATGCAAGA AATGATTGAG
    CTTTCTAGCC AGCAGCAGAA AGAGCAGCAG CTGCCTCCCC GTGCCGTACA GATTGTGTCG
    CACCCATTTT ATGGGAGAGT TGTACTAACT GCGGGACCAG CTCAGTTTGG AATGGACCTT
    TCTAAACATT TAGCAGGGGC ACGAGGCTTG GTAGCAAGAG CTGAGCCATA CAGCGGGTGC
    TCTGATATCA CCAATGGAGC AGCAATACAA GGCAAGATTG CACTGATGCA GAGAGGACAA
    TGTATGTTTG CTGAGAAGGC TCGCAATGTT CAGAAAGCTG GGGCCATTGG AGGTATTGTT
    ATAGATGACA ACGAAGGTAG TAGCAGTGAC ACTGCACCTC TCTTCCAAAT GGCTGGAGAT
    GGGAAGAATA CGGATGACAT TACCATCCCC ATGTTATTTC TCTTCAGCAA GGAAGGAAAT
    ATCATTCTTG ATGCAATTCG TGAATACCAG CAGGTGGAGG TTCTACTCTC CGATAAAGCC
    AAAGACCGAG CAACCATTTT TAAAGATTTA GAAAACGAAG GTGGAGAACA GAAGCCAGTA
    GAAAATGATT CCCAGAAACA GGCTTTAGAC GATATGTTTA TGACCCCAGA AGAAATTGAA
    GAGCTTTCTC TAGTGCCTCT GGAAGAATTG CCTGTTTCTC AGCTAGAGGT GCCAAGTTCT
    GACTCTCCAT CTGGTGGGGG CAGCACTTTA GAAAGTGACA TTACAGCAGA AAGTAAAGAC
    CATAAGACTG AAGAAACTGC ACACAGCTCT AAAGACAATT TCCAGACCCC ACCAGAAACC
    TCTGAAGACT CCACAGAGGA AAAATGGGAC AACAAAGTTC GGCCAATGGA GTCAATATTG
    GCAGACTGGA AAGAGGACAT TGAGGCCTTT GAAATGATGG AGAAGGATGA ACTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa57355
    Clone ID Related Accession (Same CDS sequence) XM_018093640.2
    Accession Version XM_018093640.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2757bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018093640.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGGCTGCC CAGTAATAGA GCCCCGCCGC TGGGGTGACA TGTGGCTAGT GCTGGCCTTC 
    TGTCTGCTGG GACAGGGACG CGCTGCTGTG ACCAAGGAGG AGAAGGCTCA GTTAAGGAGT
    CAAGTTCTGG AGATGTTTGA TCATGCATAT GGAAGTTATA TGCAACATGC TTATCCAGCT
    GATGAACTCA TGCCCTTAAC CTGCCGTGGA AGAATACGTG GCCAAGAACC AAGCAGAGGG
    GATGTTGATG ATGCTTTGGG AAAATTTTCA CTAACATTAA TCGACACACT GGACACACTT
    GTGGTGTTAA ACAAAACCAA TGAGTTTGAA GATGCAGTCC GAAAAGTGAT TACTGATGTC
    AATTTAGACA ATGATATAGT GGTGTCTGTC TTTGAGACAA ATATCAGAGT CTTGGGTGGC
    CTCCTAGGCG GACACTCACT TGCCATCATG CTGAAAGAAA ACAGCGATCG AATGCAGTGG
    TACAATGATG AACTCTTGCA CATGGCTAAG GAGCTTGGCT ATAAGCTCCT TCCTGCGTTT
    AACACCACCA GTGGCCTTCC CTACCCCAGA ATTAATTTGA AATTTGGCAT CAGAAGTCCA
    GAGGCTCGGA CGGGGACAGA GACCGATACA TGCACAGCTT GTGCAGGGAC AATGATCTTG
    GAATTCGCTG CACTGAGCCG CTTTACTGGA ATAGCCGTAT TTGAGGAACA TGCCAGGAAA
    GCCCTCGATT TTCTCTGGGA GAAAAGGCAG CGCAGTAGCA ACTTGGTCGG TGTGACCATT
    AATATCCATA CAGGGGACTG GGTACGCAAA GACAGTGGTG TGGGAGCTGG AATAGATTCT
    TACTATGAAT ATTTACTGAA GGCCTACGTT CTCCTGGGTG ATGACAGCTA TCTTGAAAGA
    TTTAATACAC ACTATGATGC CATTATGCGA TATATAAGCC AGCCTCCGCT TCTGCTTGAT
    GTACATATCC ACAAACCAAT GCTGACAGCA CGAACATGGA TGGATTCACT GCTTGCATTT
    TTTCCTGGTC TCCAGGTTTT GAAGGGGGAC ATCAGACCTG CAATTGAGAC TCATGAAATG
    CTTTACCAGG TGATAAAAAA GCACAATTTC CTGCCAGAGG CCTTTACAAC GGATTTCAGG
    GTTCACTGGG CACAGCACCC TTTAAGACCA GAATTTGCTG AAAGTACTTA TTTCCTTTAT
    AAAGCCACTG GTGATCCTTA CTACCTCGAA GTGGGAAGAA CTCTAATAGA TAATCTGAAT
    AAATATGCAC GGGTGCCATG TGGATTTGCT GCTGTTAAAG ATGTGAGAAC TGGTAGCCAT
    GAAGACCGAA TGGACTCCTT TTTCCTGGCA GAAATGTTTA AGTACCTCTA TTTGCTCTTC
    TCAGAAAGGG AAGACCTTAT ATTTGATATA GAAAGTTATA TATTTACTAC AGAAGCCCAT
    CTCCTACCTC TCTCTCTTTC CACTGCAAGT CAGACCAGCA CAAAAAAAAA CACAACTTCT
    CAATACACCG AACTAGATGA TAGTAACTTT GATTGGTCTT GCCCAAACAC CCAGATCCTC
    TTCCGAAATG ACCCAATGTA TGCACAGAAT ATTCGGGAAC CTGTAAAAAA TGTTGTTGAT
    AAGAATTGCC CCCGGAGTCC TTCTCGTCTA GATGAGGTTT CTGGCAGTGG AAATAAGCCA
    CCGCTGCGGG CTCGAGATTT CATGGCCACT AATTCAGAAC ATTTAGAGAT TCTGAAGAAG
    ATGGGCGTGA GTCTGATTCA CCTCAAGGAT GGCCGTGTTC AGTTGGTCCA GCATGCCAAC
    CAAGCTGCAA GCTCAATTGA TGCTGAGGAT GGGCTTCGAT TTATGCAAGA AATGATTGAG
    CTTTCTAGCC AGCAGCAGAA AGAGCAGCAG CTGCCTCCCC GTGCCGTACA GATTGTGTCG
    CACCCATTTT ATGGGAGAGT TGTACTAACT GCGGGACCAG CTCAGTTTGG AATGGACCTT
    TCTAAACATT TAGCAGGGGC ACGAGGCTTG GTAGCAAGAG CTGAGCCATA CAGCGGGTGC
    TCTGATATCA CCAATGGAGC AGCAATACAA GGCAAGATTG CACTGATGCA GAGAGGACAA
    TGTATGTTTG CTGAGAAGGC TCGCAATGTT CAGAAAGCTG GGGCCATTGG AGGTATTGTT
    ATAGATGACA ACGAAGGTAG TAGCAGTGAC ACTGCACCTC TCTTCCAAAT GGCTGGAGAT
    GGGAAGAATA CGGATGACAT TACCATCCCC ATGTTATTTC TCTTCAGCAA GGAAGGAAAT
    ATCATTCTTG ATGCAATTCG TGAATACCAG CAGGTGGAGG TTCTACTCTC CGATAAAGCC
    AAAGACCGAG CAACCATTTT TAAAGATTTA GAAAACGAAG GTGGAGAACA GAAGCCAGTA
    GAAAATGATT CCCAGAAACA GGCTTTAGAC GATATGTTTA TGACCCCAGA AGAAATTGAA
    GAGCTTTCTC TAGTGCCTCT GGAAGAATCG CCTGTTTCTC AGCTAGAGGT GCCAAGTTCT
    GACTCTCCAT CTGGTGGGGG CAGCACTTTA GAAAGTGACA TTACAGCAGA AAGTAAAGAC
    CATAAGACTG AAGAAACTGC ACACAGCTCT AAAGACAATT TCCAGACCCC ACCAGAAACC
    TCTGAAGACT CCACAGAGGA AAAATGGGAC AACAAAGTTC GGCCAATGGA GTCAATATTG
    GCAGACTGGA AAGAGGACAT TGAGGCCTTT GAAATGATGG AGAAGGATGA ACTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017949129.2
    CDS256..3012
    Translation

    Target ORF information:

    RefSeq Version XM_018093640.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ER degradation enhancer, mannosidase alpha-like 3 (edem3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018093640.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGGCTGCC CAGTAATAGA GCCCCGCCGC TGGGGTGACA TGTGGCTAGT GCTGGCCTTC 
    TGTCTGCTGG GACAGGGACG CGCTGCTGTG ACCAAGGAGG AGAAGGCTCA GTTAAGGAGT
    CAAGTTCTGG AGATGTTTGA TCATGCATAT GGAAGTTATA TGCAACATGC TTATCCAGCT
    GATGAACTCA TGCCCTTAAC CTGCCGTGGA AGAATACGTG GCCAAGAACC AAGCAGAGGG
    GATGTTGATG ATGCTTTGGG AAAATTTTCA CTAACATTAA TCGACACACT GGACACACTT
    GTGGTGTTAA ACAAAACCAA TGAGTTTGAA GATGCAGTCC GAAAAGTGAT TACTGATGTC
    AATTTAGACA ATGATATAGT GGTGTCTGTC TTTGAGACAA ATATCAGAGT CTTGGGTGGC
    CTCCTAGGCG GACACTCACT TGCCATCATG CTGAAAGAAA ACAGCGATCG AATGCAGTGG
    TACAATGATG AACTCTTGCA CATGGCTAAG GAGCTTGGCT ATAAGCTCCT TCCTGCGTTT
    AACACCACCA GTGGCCTTCC CTACCCCAGA ATTAATTTGA AATTTGGCAT CAGAAGTCCA
    GAGGCTCGGA CGGGGACAGA GACCGATACA TGCACAGCTT GTGCAGGGAC AATGATCTTG
    GAATTCGCTG CACTGAGCCG CTTTACTGGA ATAGCCGTAT TTGAGGAACA TGCCAGGAAA
    GCCCTCGATT TTCTCTGGGA GAAAAGGCAG CGCAGTAGCA ACTTGGTCGG TGTGACCATT
    AATATCCATA CAGGGGACTG GGTACGCAAA GACAGTGGTG TGGGAGCTGG AATAGATTCT
    TACTATGAAT ATTTACTGAA GGCCTACGTT CTCCTGGGTG ATGACAGCTA TCTTGAAAGA
    TTTAATACAC ACTATGATGC CATTATGCGA TATATAAGCC AGCCTCCGCT TCTGCTTGAT
    GTACATATCC ACAAACCAAT GCTGACAGCA CGAACATGGA TGGATTCACT GCTTGCATTT
    TTTCCTGGTC TCCAGGTTTT GAAGGGGGAC ATCAGACCTG CAATTGAGAC TCATGAAATG
    CTTTACCAGG TGATAAAAAA GCACAATTTC CTGCCAGAGG CCTTTACAAC GGATTTCAGG
    GTTCACTGGG CACAGCACCC TTTAAGACCA GAATTTGCTG AAAGTACTTA TTTCCTTTAT
    AAAGCCACTG GTGATCCTTA CTACCTCGAA GTGGGAAGAA CTCTAATAGA TAATCTGAAT
    AAATATGCAC GGGTGCCATG TGGATTTGCT GCTGTTAAAG ATGTGAGAAC TGGTAGCCAT
    GAAGACCGAA TGGACTCCTT TTTCCTGGCA GAAATGTTTA AGTACCTCTA TTTGCTCTTC
    TCAGAAAGGG AAGACCTTAT ATTTGATATA GAAAGTTATA TATTTACTAC AGAAGCCCAT
    CTCCTACCTC TCTCTCTTTC CACTGCAAGT CAGACCAGCA CAAAAAAAAA CACAACTTCT
    CAATACACCG AACTAGATGA TAGTAACTTT GATTGGTCTT GCCCAAACAC CCAGATCCTC
    TTCCGAAATG ACCCAATGTA TGCACAGAAT ATTCGGGAAC CTGTAAAAAA TGTTGTTGAT
    AAGAATTGCC CCCGGAGTCC TTCTCGTCTA GATGAGGTTT CTGGCAGTGG AAATAAGCCA
    CCGCTGCGGG CTCGAGATTT CATGGCCACT AATTCAGAAC ATTTAGAGAT TCTGAAGAAG
    ATGGGCGTGA GTCTGATTCA CCTCAAGGAT GGCCGTGTTC AGTTGGTCCA GCATGCCAAC
    CAAGCTGCAA GCTCAATTGA TGCTGAGGAT GGGCTTCGAT TTATGCAAGA AATGATTGAG
    CTTTCTAGCC AGCAGCAGAA AGAGCAGCAG CTGCCTCCCC GTGCCGTACA GATTGTGTCG
    CACCCATTTT ATGGGAGAGT TGTACTAACT GCGGGACCAG CTCAGTTTGG AATGGACCTT
    TCTAAACATT TAGCAGGGGC ACGAGGCTTG GTAGCAAGAG CTGAGCCATA CAGCGGGTGC
    TCTGATATCA CCAATGGAGC AGCAATACAA GGCAAGATTG CACTGATGCA GAGAGGACAA
    TGTATGTTTG CTGAGAAGGC TCGCAATGTT CAGAAAGCTG GGGCCATTGG AGGTATTGTT
    ATAGATGACA ACGAAGGTAG TAGCAGTGAC ACTGCACCTC TCTTCCAAAT GGCTGGAGAT
    GGGAAGAATA CGGATGACAT TACCATCCCC ATGTTATTTC TCTTCAGCAA GGAAGGAAAT
    ATCATTCTTG ATGCAATTCG TGAATACCAG CAGGTGGAGG TTCTACTCTC CGATAAAGCC
    AAAGACCGAG CAACCATTTT TAAAGATTTA GAAAACGAAG GTGGAGAACA GAAGCCAGTA
    GAAAATGATT CCCAGAAACA GGCTTTAGAC GATATGTTTA TGACCCCAGA AGAAATTGAA
    GAGCTTTCTC TAGTGCCTCT GGAAGAATCG CCTGTTTCTC AGCTAGAGGT GCCAAGTTCT
    GACTCTCCAT CTGGTGGGGG CAGCACTTTA GAAAGTGACA TTACAGCAGA AAGTAAAGAC
    CATAAGACTG AAGAAACTGC ACACAGCTCT AAAGACAATT TCCAGACCCC ACCAGAAACC
    TCTGAAGACT CCACAGAGGA AAAATGGGAC AACAAAGTTC GGCCAATGGA GTCAATATTG
    GCAGACTGGA AAGAGGACAT TGAGGCCTTT GAAATGATGG AGAAGGATGA ACTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa57356
    Clone ID Related Accession (Same CDS sequence) XM_002932144.5
    Accession Version XM_002932144.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2742bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002932144.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGGCTGCC CAGTAATAGA GCCCCGCCGC TGGGGTGACA TGTGGCTAGT GCTGGCCTTC 
    TGTCTGCTGG GACAGGGACG CGCTGCTGTG ACCAAGGAGG AGAAGGCTCA GTTAAGGAGT
    CAAGTTCTGG AGATGTTTGA TCATGCATAT GGAAGTTATA TGCAACATGC TTATCCAGCT
    GATGAACTCA TGCCCTTAAC CTGCCGTGGA AGAATACGTG GCCAAGAACC AAGCAGAGGG
    GATGTTGATG ATGCTTTGGG AAAATTTTCA CTAACATTAA TCGACACACT GGACACACTT
    GTGGTGTTAA ACAAAACCAA TGAGTTTGAA GATGCAGTCC GAAAAGTGAT TACTGATGTC
    AATTTAGACA ATGATATAGT GGTGTCTGTC TTTGAGACAA ATATCAGAGT CTTGGGTGGC
    CTCCTAGGCG GACACTCACT TGCCATCATG CTGAAAGAAA ACAGCGATCG AATGCAGTGG
    TACAATGATG AACTCTTGCA CATGGCTAAG GAGCTTGGCT ATAAGCTCCT TCCTGCGTTT
    AACACCACCA GTGGCCTTCC CTACCCCAGA ATTAATTTGA AATTTGGCAT CAGAAGTCCA
    GAGGCTCGGA CGGGGACAGA GACCGATACA TGCACAGCTT GTGCAGGGAC AATGATCTTG
    GAATTCGCTG CACTGAGCCG CTTTACTGGA ATAGCCGTAT TTGAGGAACA TGCCAGGAAA
    GCCCTCGATT TTCTCTGGGA GAAAAGGCAG CGCAGTAGCA ACTTGGTCGG TGTGACCATT
    AATATCCATA CAGGGGACTG GGTACGCAAA GACAGTGGTG TGGGAGCTGG AATAGATTCT
    TACTATGAAT ATTTACTGAA GGCCTACGTT CTCCTGGGTG ATGACAGCTA TCTTGAAAGA
    TTTAATACAC ACTATGATGC CATTATGCGA TATATAAGCC AGCCTCCGCT TCTGCTTGAT
    GTACATATCC ACAAACCAAT GCTGACAGCA CGAACATGGA TGGATTCACT GCTTGCATTT
    TTTCCTGGTC TCCAGGTTTT GAAGGGGGAC ATCAGACCTG CAATTGAGAC TCATGAAATG
    CTTTACCAGG TGATAAAAAA GCACAATTTC CTGCCAGAGG CCTTTACAAC GGATTTCAGG
    GTTCACTGGG CACAGCACCC TTTAAGACCA GAATTTGCTG AAAGTACTTA TTTCCTTTAT
    AAAGCCACTG GTGATCCTTA CTACCTCGAA GTGGGAAGAA CTCTAATAGA TAATCTGAAT
    AAATATGCAC GGGTGCCATG TGGATTTGCT GCTGTTAAAG ATGTGAGAAC TGGTAGCCAT
    GAAGACCGAA TGGACTCCTT TTTCCTGGCA GAAATGTTTA AGTACCTCTA TTTGCTCTTC
    TCAGAAAGGG AAGACCTTAT ATTTGATATA GAAAGTTATA TATTTACTAC AGAAGCCCAT
    CTCCTACCTC TCTCTCTTTC CACTGCAAGT CAGACCAGCA CAAAAAAAAA CACAACTTCT
    CAATACACCG AACTAGATGA TAGTAACTTT GATTGGTCTT GCCCAAACAC CCAGATCCTC
    TTCCGAAATG ACCCAATGTA TGCACAGAAT ATTCGGGAAC CTGTAAAAAA TGTTGTTGAT
    AAGAATTGCC CCCGGAGTCC TTCTCGTCTA GATGAGGTTT CTGGCAGTGG AAATAAGCCA
    CCGCTGCGGG CTCGAGATTT CATGGCCACT AATTCAGAAC ATTTAGAGAT TCTGAAGAAG
    ATGGGCGTGA GTCTGATTCA CCTCAAGGAT GGCCGTGTTC AGTTGGTCCA GCATGCCAAC
    CAAGCTGCAA GCTCAATTGA TGCTGAGGAT GGGCTTCGAT TTATGCAAGA AATGATTGAG
    CTTTCTAGCC AGCAGCAGAA AGAGCAGCAG CTGCCTCCCC GTGCCGTACA GATTGTGTCG
    CACCCATTTT ATGGGAGAGT TGTACTAACT GCGGGACCAG CTCAGTTTGG AATGGACCTT
    TCTAAACATT TAGCAGGGGC ACGAGGCTTG GTAGCAAGAG CTGAGCCATA CAGCGGGTGC
    TCTGATATCA CCAATGGAGC AGCAATACAA GGCAAGATTG CACTGATGCA GAGAGGACAA
    TGTATGTTTG CTGAGAAGGC TCGCAATGTT CAGAAAGCTG GGGCCATTGG AGGTATTGTT
    ATAGATGACA ACGAAGGTAG TAGCAGTGAC ACTGCACCTC TCTTCCAAAT GGCTGGAGAT
    GGGAAGAATA CGGATGACAT TACCATCCCC ATGTTATTTC TCTTCAGCAA GGAAGGAAAT
    ATCATTCTTG ATGCAATTCG TGAATACCAG CAGGTGGAGG TTCTACTCTC CGATAAAGCC
    AAAGACCGAG ATTTAGAAAA CGAAGGTGGA GAACAGAAGC CAGTAGAAAA TGATTCCCAG
    AAACAGGCTT TAGACGATAT GTTTATGACC CCAGAAGAAA TTGAAGAGCT TTCTCTAGTG
    CCTCTGGAAG AATCGCCTGT TTCTCAGCTA GAGGTGCCAA GTTCTGACTC TCCATCTGGT
    GGGGGCAGCA CTTTAGAAAG TGACATTACA GCAGAAAGTA AAGACCATAA GACTGAAGAA
    ACTGCACACA GCTCTAAAGA CAATTTCCAG ACCCCACCAG AAACCTCTGA AGACTCCACA
    GAGGAAAAAT GGGACAACAA AGTTCGGCCA ATGGAGTCAA TATTGGCAGA CTGGAAAGAG
    GACATTGAGG CCTTTGAAAT GATGGAGAAG GATGAACTAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002932190.3
    CDS255..2996
    Translation

    Target ORF information:

    RefSeq Version XM_002932144.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ER degradation enhancer, mannosidase alpha-like 3 (edem3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002932144.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGGCTGCC CAGTAATAGA GCCCCGCCGC TGGGGTGACA TGTGGCTAGT GCTGGCCTTC 
    TGTCTGCTGG GACAGGGACG CGCTGCTGTG ACCAAGGAGG AGAAGGCTCA GTTAAGGAGT
    CAAGTTCTGG AGATGTTTGA TCATGCATAT GGAAGTTATA TGCAACATGC TTATCCAGCT
    GATGAACTCA TGCCCTTAAC CTGCCGTGGA AGAATACGTG GCCAAGAACC AAGCAGAGGG
    GATGTTGATG ATGCTTTGGG AAAATTTTCA CTAACATTAA TCGACACACT GGACACACTT
    GTGGTGTTAA ACAAAACCAA TGAGTTTGAA GATGCAGTCC GAAAAGTGAT TACTGATGTC
    AATTTAGACA ATGATATAGT GGTGTCTGTC TTTGAGACAA ATATCAGAGT CTTGGGTGGC
    CTCCTAGGCG GACACTCACT TGCCATCATG CTGAAAGAAA ACAGCGATCG AATGCAGTGG
    TACAATGATG AACTCTTGCA CATGGCTAAG GAGCTTGGCT ATAAGCTCCT TCCTGCGTTT
    AACACCACCA GTGGCCTTCC CTACCCCAGA ATTAATTTGA AATTTGGCAT CAGAAGTCCA
    GAGGCTCGGA CGGGGACAGA GACCGATACA TGCACAGCTT GTGCAGGGAC AATGATCTTG
    GAATTCGCTG CACTGAGCCG CTTTACTGGA ATAGCCGTAT TTGAGGAACA TGCCAGGAAA
    GCCCTCGATT TTCTCTGGGA GAAAAGGCAG CGCAGTAGCA ACTTGGTCGG TGTGACCATT
    AATATCCATA CAGGGGACTG GGTACGCAAA GACAGTGGTG TGGGAGCTGG AATAGATTCT
    TACTATGAAT ATTTACTGAA GGCCTACGTT CTCCTGGGTG ATGACAGCTA TCTTGAAAGA
    TTTAATACAC ACTATGATGC CATTATGCGA TATATAAGCC AGCCTCCGCT TCTGCTTGAT
    GTACATATCC ACAAACCAAT GCTGACAGCA CGAACATGGA TGGATTCACT GCTTGCATTT
    TTTCCTGGTC TCCAGGTTTT GAAGGGGGAC ATCAGACCTG CAATTGAGAC TCATGAAATG
    CTTTACCAGG TGATAAAAAA GCACAATTTC CTGCCAGAGG CCTTTACAAC GGATTTCAGG
    GTTCACTGGG CACAGCACCC TTTAAGACCA GAATTTGCTG AAAGTACTTA TTTCCTTTAT
    AAAGCCACTG GTGATCCTTA CTACCTCGAA GTGGGAAGAA CTCTAATAGA TAATCTGAAT
    AAATATGCAC GGGTGCCATG TGGATTTGCT GCTGTTAAAG ATGTGAGAAC TGGTAGCCAT
    GAAGACCGAA TGGACTCCTT TTTCCTGGCA GAAATGTTTA AGTACCTCTA TTTGCTCTTC
    TCAGAAAGGG AAGACCTTAT ATTTGATATA GAAAGTTATA TATTTACTAC AGAAGCCCAT
    CTCCTACCTC TCTCTCTTTC CACTGCAAGT CAGACCAGCA CAAAAAAAAA CACAACTTCT
    CAATACACCG AACTAGATGA TAGTAACTTT GATTGGTCTT GCCCAAACAC CCAGATCCTC
    TTCCGAAATG ACCCAATGTA TGCACAGAAT ATTCGGGAAC CTGTAAAAAA TGTTGTTGAT
    AAGAATTGCC CCCGGAGTCC TTCTCGTCTA GATGAGGTTT CTGGCAGTGG AAATAAGCCA
    CCGCTGCGGG CTCGAGATTT CATGGCCACT AATTCAGAAC ATTTAGAGAT TCTGAAGAAG
    ATGGGCGTGA GTCTGATTCA CCTCAAGGAT GGCCGTGTTC AGTTGGTCCA GCATGCCAAC
    CAAGCTGCAA GCTCAATTGA TGCTGAGGAT GGGCTTCGAT TTATGCAAGA AATGATTGAG
    CTTTCTAGCC AGCAGCAGAA AGAGCAGCAG CTGCCTCCCC GTGCCGTACA GATTGTGTCG
    CACCCATTTT ATGGGAGAGT TGTACTAACT GCGGGACCAG CTCAGTTTGG AATGGACCTT
    TCTAAACATT TAGCAGGGGC ACGAGGCTTG GTAGCAAGAG CTGAGCCATA CAGCGGGTGC
    TCTGATATCA CCAATGGAGC AGCAATACAA GGCAAGATTG CACTGATGCA GAGAGGACAA
    TGTATGTTTG CTGAGAAGGC TCGCAATGTT CAGAAAGCTG GGGCCATTGG AGGTATTGTT
    ATAGATGACA ACGAAGGTAG TAGCAGTGAC ACTGCACCTC TCTTCCAAAT GGCTGGAGAT
    GGGAAGAATA CGGATGACAT TACCATCCCC ATGTTATTTC TCTTCAGCAA GGAAGGAAAT
    ATCATTCTTG ATGCAATTCG TGAATACCAG CAGGTGGAGG TTCTACTCTC CGATAAAGCC
    AAAGACCGAG ATTTAGAAAA CGAAGGTGGA GAACAGAAGC CAGTAGAAAA TGATTCCCAG
    AAACAGGCTT TAGACGATAT GTTTATGACC CCAGAAGAAA TTGAAGAGCT TTCTCTAGTG
    CCTCTGGAAG AATCGCCTGT TTCTCAGCTA GAGGTGCCAA GTTCTGACTC TCCATCTGGT
    GGGGGCAGCA CTTTAGAAAG TGACATTACA GCAGAAAGTA AAGACCATAA GACTGAAGAA
    ACTGCACACA GCTCTAAAGA CAATTTCCAG ACCCCACCAG AAACCTCTGA AGACTCCACA
    GAGGAAAAAT GGGACAACAA AGTTCGGCCA ATGGAGTCAA TATTGGCAGA CTGGAAAGAG
    GACATTGAGG CCTTTGAAAT GATGGAGAAG GATGAACTAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.