shroom1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	shroom1
Entrez Gene ID	100145627
Full Name	shroom family member 1
Synonyms	Xshroom1,apx
General protein information	Preferred Names shroom family member 1 Names protein Shroom1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004912831.3	XP_004912888.1	protein Shroom1
XM_004912832.3	XP_004912889.1	protein Shroom1
XM_004912831.4	XP_004912888.2	protein Shroom1
XM_002935260.4	XP_002935306.2	protein Shroom1
XM_004912832.4	XP_004912889.2	protein Shroom1
XM_002935260.5	XP_002935306.3	protein Shroom1

Pathways from BioSystems
Gene Ontology

The following shroom1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the shroom1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa54485	XM_004912832.4 Latest version!	Xenopus tropicalis shroom family member 1 (shroom1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa54485	XM_004912831.4 Latest version!	Xenopus tropicalis shroom family member 1 (shroom1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa54485	XM_002935260.5 Latest version!	Xenopus tropicalis shroom family member 1 (shroom1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa07579	XM_004912832.3 Latest version!	Xenopus tropicalis shroom family member 1 (shroom1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa07579	XM_004912831.3 Latest version!	Xenopus tropicalis shroom family member 1 (shroom1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa07579	XM_002935260.4 Latest version!	Xenopus tropicalis shroom family member 1 (shroom1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

shroom1 ( XM_004912831.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912888.2 Xenopus tropicalis shroom family member 1 (shroom1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa54485
Clone ID Related Accession (Same CDS sequence)	XM_004912831.4 , XM_004912832.4 , XM_002935260.5
Accession Version	XM_004912831.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein Shroom1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004912831.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCAT TTGGTAATGC AATTGAGAGA TGGAACATAA AAAGTACAGG AGTCGTTGCA 
GGTCTGGGAC ATTCTGAAAG AATATCCCCC ATAAGATCTA TGACCACATT GGTGGATTCG 
GCCTATAGCT CATTTTCTGG CAGTTCGTAT GTTCCAGAAT ACCAAAACTC ATTTCAGCAT 
GACAGCTGTC ACTTTAATGA TGAACAGCTT TCTTACATGG ACTCTGAATA TGTAAGAGCA 
ATCTATAATC CTAGTGTAGT AGACATGGAT GGTGTATATC ATGATATATT GTCAGGACAT 
AGATGCTCGA ATGTAGCACT ATCTGGAAGA ACTAGCAGTG CCAGCTGTGA CCACCCATCA 
CAAGTGCATC AAACTTCACC AGCAAAACTG GATAACTATC TTACTAACCT TGGTTCTGAC 
CAGTTCAGTG GGACTTATGG GGAATCCATA AATATGAAGC ATAAGCAGAA TCACTCTAAT 
AGTATATATA TGCCTAACCA CAAAGCATAT GGCCTCCAAC GTAACAGTCC TACAGGAGTA 
AGCAGTTTGC AGGAAAAAGA AAACCAAATG TATAATCAAT CAAATTATAT GGAAATAAAA 
CATTATTTTA GTAGATCCTT AGATGTTCTT CAAACAGAAG GAGAGCTCAA GACACAAACC 
TCATGTACTC AGAATCTGCC GGATGAGGTC AGCTGCAAGT TCAGAAACAC ACATTCTACA 
AATAGAACGA GCAAAGACCT TTCCAAGGGA AACGATCTAC AAAAAAGCAA TGAAGAAAAC 
ACAGAGAGAG ACAGCCCATA TCTGACAAAA GAGGGTCCAG TTTCTCAAGG TCAATATGCA 
TCAGATGTAA GGACAAGTTT TAAAAACATA AGACGCTCCC TTAAAAAGTC TGTGTCTGAA 
AAAATTGGTG CTCATGACAA TAAAGGGGGT TGCTGGATTA TGAAGCCAGG TCAAGACCCT 
CATTGTTATA ATTCTGAGGG AACAGTTAAG GACGCAGAGT ATGATGACGT CGAGCAGTGG 
GATATCAGGA AATCAAGACT TAATACAAGG GCAAGCCAGA CTTTATATTG TGAGTCTAAT 
AAAGATGCCT CTTGCCCTCC CTTGAATGCT TTGAATTCTA AGAATGAAGT TCTCGAAGCT 
GATAATACAC TCTGTTTTCA AAAGGATGCA ACTGTAAAGT CTATTCCCCT ATTATCTCAG 
CATATTCAAC AGGAAAAATC TAAAAGCCAT CCCCTGTCTG ATTTAAACTG TGAGAAGATT 
ACCAAGGCAA CAACACCTAT GCTGTATCAT CTTGCTGGAG GAAGGCATAG TGCCTTTATA 
GCCCCTGTAC ACAATACAAA TCCAGCTAAA CAGGAAAAAC TCAAGCTTGA GTCAAAGACA 
TTTGAAAGAA CGAATAACTT CTCTATACTA CAACAAACCG AGCCAAGACC AGATAACCAT 
AAATTCCCCA AGAATAAAAG CCTAACACAA CTTGCTGATC TCCATGACTC TGCAGAGGGT 
GGGAATTCTG GGAATTTAAA TTCTTCAGCA GAAGAGTGCC TAATGAATGA CTACATTGAG 
AAACTGAAGG TTGCCCAGAA GAAAGTATTG AGGGAAACCT CTTTTAAAAG AAAGGACTTG 
CAGATGAGTT TGCCTTGCAG ATTCAAGTTG AATCCACCCA AAAGGCCTAC CATTGATCAT 
TTTCGATCCT ATTCTTCATC CAGTGCAAAT GAGGAGACGG CATTTCTCCA AACAAAAAAC 
TCTGTTGACA GCACCTACAA CAAAGATGAT GCTGAAAAGG TCGCAGTTAC TCGAATAGGA 
GGTCGGAAAC GGATTACAAA GGAACAAAAG AAGCTGTGTT ATTCAGAACC AGAGAAATTA 
GATCACCTTG GGATTCAAAA ATCTAACTTT GCATGGAAGG AGGAACCTGC TATCGCAAAT 
AAAAGGGAGA TGAGTGATTC TGATGTTGCT GCAAATAAGA TAAAGTATCT GGAGAGCAAA 
GAACGAACTG CCTCATCTTC AAACCTTTCT AAGACAGAAC TCAAACAGAT CCAGCATAAT 
GCGCTTGTTG AATATATGGA AAGGAAAACA AACCAAAGGC CCAGCAGCAA TCCACAATTC 
CAGATGGAAA GGACATCGGG ATTGCCAAAT TATAGTGAGT GGAATATCTA CTCATCTGAA 
ACCAGTTCTG GTGATGGTTC ACAAAAGTAC CTACGGAGGA GATCAGCTGG AGCTTCATCT 
TCCTATGATG CCACAGTAAC ATGGAATGAT AGATTTGGAA AGACTTCACC TCTTGGGCGG 
CGCTCTGCTG TGGAACCAAC TGCTGGTGTG CAACGCAGGA GTTTTTTTGA TCAACGCACT 
TTAGATGGCA GTCAAGAACG TCTTGAAGGT TCTTCCCCAT CTCTCAGTCA AAAAACATCA 
AAGTCAAGTC ATCGTGAACA GGTCTCGTAT GTAAATATGG AATTTCTTCC CTCCGCACTT 
TCCAAGAATC ACATTTATAA TGACCGGCTG ACTGTACCCG GGGACAACAC CAGCGCCGAC 
ACTGAGAGAA TTCGTGCTTC CAAAAGCAGA GGGAAGTCTA TGGAAGAAAT AGGGACTGCA 
GACATTGTAA CGCTTGCAGA GCTGAGCCAT AGCTCAGATC AACTTTACCA CATTAAAGGG 
CCTGTGATAT CGCCAAGGCT GGAAAACACA AGGACTGCAG CTGTGTCCCA TCAAGACAAC 
GTCTTGGCTA GCACTCAGAA CGAAACAGGA AATCTGCCCA GACAAACACA TCAAGAAAGT 
GTTCTGGGGC CCCGCAGATC AGACTTAGCA AATTTGGGAC AAGAGGCACA TTCCTGGCCC 
TTGAGAGCTA GTGCTGCTTC CTCAGGCATA GATAATCCCT GCTCTTCTCC TCCTTCGGCA 
AAGGTTCACC CCTGGGCTCC TGAGCCATTG CATTGCTTGG ACACAGAAGA TGAGGTCTTT 
ACACAAGCCA GCACTGCCAG AAATGAAGAT CCCAGCTCCA CTGCCCTTAG CCATCTGCAC 
GATATTGGAA ACCCTGTGTG TGAAGAGGAA GATACTGCAT TATGCTTTTC TTTCTTACCT 
GAGCAGGGTC ATCTAGAACG GCTGATTGTC AATGAGGCTG CACCAAGCTC CGAGACTGAT 
GAAGGTGTTT TGGACACTGC TGCTGTGAAT GCGACGACAA CGCAACCTCC TGAGACTTCA 
GAAGACAACA AGCCACATGG CTTTAGTGCT GAGAATCAAG AAGTTGAAGA AGGCAGAGAA 
CCTATGCTAG CAGAGTTTGT AGACAGCTCA CAGCAGCTAG AGTTGAGGTC CCTTCCTTCT 
AGCCAGGTTA ACGTAATGCA GACTACAGAG CCATATGTCG GTGATGACAA GAGCAGTGGA 
AATGAAGATT CCATGGAGCA AAAATCACAG GACATTGAGC AGAAAAGTAA GGAACCAGAA 
GAGGAAGATT TACCCAAGGT GAAGTTAAAA TCTCCAGAAG ATGAAAGACG AGAGGAACTG 
GTTAAAGACA TAGTTGCCAA GGATAAATCT CTGATGAATT GTCTCCAGCC GATTTCTGTC 
AGAGAATCTG CTATGGATCT CATGAAAAGC CTCTTCCCTA TGGACTTCAC TGAAGCGGAA 
AAATCTAGAA CTAGAGGACT TCTTAGAAAA GACAACAGTG AAACATTAAG GAAAAACAAT 
TCAGACCTTG AAAGTTCTTC TAAACTTCCT TCAAAAATAA CCGTGATGCT TCAGAAGAGT 
TCTGCGCGGA GGCCAGATGG GGAAAGCCTA GATGACATCA CTTCAAAGAA GATGGAACTT 
CTTTCTAACA TCGGTTCCAA GCTAGAGGAC CTTTGTGAGC AAAAAGAAAT CCTTTTGGCT 
GATAATAGTA AGAACACGAC GCACGGCAAT AATATTCAGG CACTAGTGAA AGAGTTGTGT 
AAGCCCAATG AGTTTGAGCG CTACATGATG TTCATTGGGG ATCTGGAGAA AGTGGTGAGC 
CTGCTATTTT CCCTGTCGAC AAGGCTGACT CGGGTTGAAA ATGCTTTAAG CAAGGTTGAT 
GAAAATACAG ATGCAGAAGA GATGCAATCC CTAAAGGAGC GCCACAATCT CCTGTCCAGT 
CAAAGAGAAG ATGCCAAAGA CTTAAAGGCA AACTTAGACC GCAGAGAACA AGTTGTCACG 
GGAATCCTTG TAAAATACCT GAATGAGGAG CAGCTGCAGG ACTACAAGCA CTTTGTCAGA 
CTGAAGACCT CCCTCCTAAT TGAACAAAAG AACCTGGAGG AGAAGATAAA ACTGTACGAG 
GAGCAGTTAG AGAGCATCCA CGATAGCATC CCACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912888.2
CDS	394..4692
Translation	MSSFGNAIERWNIKSTGVVAGLGHSERISPIRSMTTLVDSAYSSFSGSSYVPEYQNSFQHDSCHFNDEQLSYMDSEYVRAIYNPSVVDMDGVYHDILSGHRCSNVALSGRTSSASCDHPSQVHQTSPAKLDNYLTNLGSDQFSGTYGESINMKHKQNHSNSIYMPNHKAYGLQRNSPTGVSSLQEKENQMYNQSNYMEIKHYFSRSLDVLQTEGELKTQTSCTQNLPDEVSCKFRNTHSTNRTSKDLSKGNDLQKSNEENTERDSPYLTKEGPVSQGQYASDVRTSFKNIRRSLKKSVSEKIGAHDNKGGCWIMKPGQDPHCYNSEGTVKDAEYDDVEQWDIRKSRLNTRASQTLYCESNKDASCPPLNALNSKNEVLEADNTLCFQKDATVKSIPLLSQHIQQEKSKSHPLSDLNCEKITKATTPMLYHLAGGRHSAFIAPVHNTNPAKQEKLKLESKTFERTNNFSILQQTEPRPDNHKFPKNKSLTQLADLHDSAEGGNSGNLNSSAEECLMNDYIEKLKVAQKKVLRETSFKRKDLQMSLPCRFKLNPPKRPTIDHFRSYSSSSANEETAFLQTKNSVDSTYNKDDAEKVAVTRIGGRKRITKEQKKLCYSEPEKLDHLGIQKSNFAWKEEPAIANKREMSDSDVAANKIKYLESKERTASSSNLSKTELKQIQHNALVEYMERKTNQRPSSNPQFQMERTSGLPNYSEWNIYSSETSSGDGSQKYLRRRSAGASSSYDATVTWNDRFGKTSPLGRRSAVEPTAGVQRRSFFDQRTLDGSQERLEGSSPSLSQKTSKSSHREQVSYVNMEFLPSALSKNHIYNDRLTVPGDNTSADTERIRASKSRGKSMEEIGTADIVTLAELSHSSDQLYHIKGPVISPRLENTRTAAVSHQDNVLASTQNETGNLPRQTHQESVLGPRRSDLANLGQEAHSWPLRASAASSGIDNPCSSPPSAKVHPWAPEPLHCLDTEDEVFTQASTARNEDPSSTALSHLHDIGNPVCEEEDTALCFSFLPEQGHLERLIVNEAAPSSETDEGVLDTAAVNATTTQPPETSEDNKPHGFSAENQEVEEGREPMLAEFVDSSQQLELRSLPSSQVNVMQTTEPYVGDDKSSGNEDSMEQKSQDIEQKSKEPEEEDLPKVKLKSPEDERREELVKDIVAKDKSLMNCLQPISVRESAMDLMKSLFPMDFTEAEKSRTRGLLRKDNSETLRKNNSDLESSSKLPSKITVMLQKSSARRPDGESLDDITSKKMELLSNIGSKLEDLCEQKEILLADNSKNTTHGNNIQALVKELCKPNEFERYMMFIGDLEKVVSLLFSLSTRLTRVENALSKVDENTDAEEMQSLKERHNLLSSQREDAKDLKANLDRREQVVTGILVKYLNEEQLQDYKHFVRLKTSLLIEQKNLEEKIKLYEEQLESIHDSIPP

Target ORF information:

RefSeq Version	XM_004912831.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis shroom family member 1 (shroom1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912831.4

ORF Insert Sequence:

ATGTCATCAT TTGGTAATGC AATTGAGAGA TGGAACATAA AAAGTACAGG AGTCGTTGCA 
GGTCTGGGAC ATTCTGAAAG AATATCCCCC ATAAGATCTA TGACCACATT GGTGGATTCG 
GCCTATAGCT CATTTTCTGG CAGTTCGTAT GTTCCAGAAT ACCAAAACTC ATTTCAGCAT 
GACAGCTGTC ACTTTAATGA TGAACAGCTT TCTTACATGG ACTCTGAATA TGTAAGAGCA 
ATCTATAATC CTAGTGTAGT AGACATGGAT GGTGTATATC ATGATATATT GTCAGGACAT 
AGATGCTCGA ATGTAGCACT ATCTGGAAGA ACTAGCAGTG CCAGCTGTGA CCACCCATCA 
CAAGTGCATC AAACTTCACC AGCAAAACTG GATAACTATC TTACTAACCT TGGTTCTGAC 
CAGTTCAGTG GGACTTATGG GGAATCCATA AATATGAAGC ATAAGCAGAA TCACTCTAAT 
AGTATATATA TGCCTAACCA CAAAGCATAT GGCCTCCAAC GTAACAGTCC TACAGGAGTA 
AGCAGTTTGC AGGAAAAAGA AAACCAAATG TATAATCAAT CAAATTATAT GGAAATAAAA 
CATTATTTTA GTAGATCCTT AGATGTTCTT CAAACAGAAG GAGAGCTCAA GACACAAACC 
TCATGTACTC AGAATCTGCC GGATGAGGTC AGCTGCAAGT TCAGAAACAC ACATTCTACA 
AATAGAACGA GCAAAGACCT TTCCAAGGGA AACGATCTAC AAAAAAGCAA TGAAGAAAAC 
ACAGAGAGAG ACAGCCCATA TCTGACAAAA GAGGGTCCAG TTTCTCAAGG TCAATATGCA 
TCAGATGTAA GGACAAGTTT TAAAAACATA AGACGCTCCC TTAAAAAGTC TGTGTCTGAA 
AAAATTGGTG CTCATGACAA TAAAGGGGGT TGCTGGATTA TGAAGCCAGG TCAAGACCCT 
CATTGTTATA ATTCTGAGGG AACAGTTAAG GACGCAGAGT ATGATGACGT CGAGCAGTGG 
GATATCAGGA AATCAAGACT TAATACAAGG GCAAGCCAGA CTTTATATTG TGAGTCTAAT 
AAAGATGCCT CTTGCCCTCC CTTGAATGCT TTGAATTCTA AGAATGAAGT TCTCGAAGCT 
GATAATACAC TCTGTTTTCA AAAGGATGCA ACTGTAAAGT CTATTCCCCT ATTATCTCAG 
CATATTCAAC AGGAAAAATC TAAAAGCCAT CCCCTGTCTG ATTTAAACTG TGAGAAGATT 
ACCAAGGCAA CAACACCTAT GCTGTATCAT CTTGCTGGAG GAAGGCATAG TGCCTTTATA 
GCCCCTGTAC ACAATACAAA TCCAGCTAAA CAGGAAAAAC TCAAGCTTGA GTCAAAGACA 
TTTGAAAGAA CGAATAACTT CTCTATACTA CAACAAACCG AGCCAAGACC AGATAACCAT 
AAATTCCCCA AGAATAAAAG CCTAACACAA CTTGCTGATC TCCATGACTC TGCAGAGGGT 
GGGAATTCTG GGAATTTAAA TTCTTCAGCA GAAGAGTGCC TAATGAATGA CTACATTGAG 
AAACTGAAGG TTGCCCAGAA GAAAGTATTG AGGGAAACCT CTTTTAAAAG AAAGGACTTG 
CAGATGAGTT TGCCTTGCAG ATTCAAGTTG AATCCACCCA AAAGGCCTAC CATTGATCAT 
TTTCGATCCT ATTCTTCATC CAGTGCAAAT GAGGAGACGG CATTTCTCCA AACAAAAAAC 
TCTGTTGACA GCACCTACAA CAAAGATGAT GCTGAAAAGG TCGCAGTTAC TCGAATAGGA 
GGTCGGAAAC GGATTACAAA GGAACAAAAG AAGCTGTGTT ATTCAGAACC AGAGAAATTA 
GATCACCTTG GGATTCAAAA ATCTAACTTT GCATGGAAGG AGGAACCTGC TATCGCAAAT 
AAAAGGGAGA TGAGTGATTC TGATGTTGCT GCAAATAAGA TAAAGTATCT GGAGAGCAAA 
GAACGAACTG CCTCATCTTC AAACCTTTCT AAGACAGAAC TCAAACAGAT CCAGCATAAT 
GCGCTTGTTG AATATATGGA AAGGAAAACA AACCAAAGGC CCAGCAGCAA TCCACAATTC 
CAGATGGAAA GGACATCGGG ATTGCCAAAT TATAGTGAGT GGAATATCTA CTCATCTGAA 
ACCAGTTCTG GTGATGGTTC ACAAAAGTAC CTACGGAGGA GATCAGCTGG AGCTTCATCT 
TCCTATGATG CCACAGTAAC ATGGAATGAT AGATTTGGAA AGACTTCACC TCTTGGGCGG 
CGCTCTGCTG TGGAACCAAC TGCTGGTGTG CAACGCAGGA GTTTTTTTGA TCAACGCACT 
TTAGATGGCA GTCAAGAACG TCTTGAAGGT TCTTCCCCAT CTCTCAGTCA AAAAACATCA 
AAGTCAAGTC ATCGTGAACA GGTCTCGTAT GTAAATATGG AATTTCTTCC CTCCGCACTT 
TCCAAGAATC ACATTTATAA TGACCGGCTG ACTGTACCCG GGGACAACAC CAGCGCCGAC 
ACTGAGAGAA TTCGTGCTTC CAAAAGCAGA GGGAAGTCTA TGGAAGAAAT AGGGACTGCA 
GACATTGTAA CGCTTGCAGA GCTGAGCCAT AGCTCAGATC AACTTTACCA CATTAAAGGG 
CCTGTGATAT CGCCAAGGCT GGAAAACACA AGGACTGCAG CTGTGTCCCA TCAAGACAAC 
GTCTTGGCTA GCACTCAGAA CGAAACAGGA AATCTGCCCA GACAAACACA TCAAGAAAGT 
GTTCTGGGGC CCCGCAGATC AGACTTAGCA AATTTGGGAC AAGAGGCACA TTCCTGGCCC 
TTGAGAGCTA GTGCTGCTTC CTCAGGCATA GATAATCCCT GCTCTTCTCC TCCTTCGGCA 
AAGGTTCACC CCTGGGCTCC TGAGCCATTG CATTGCTTGG ACACAGAAGA TGAGGTCTTT 
ACACAAGCCA GCACTGCCAG AAATGAAGAT CCCAGCTCCA CTGCCCTTAG CCATCTGCAC 
GATATTGGAA ACCCTGTGTG TGAAGAGGAA GATACTGCAT TATGCTTTTC TTTCTTACCT 
GAGCAGGGTC ATCTAGAACG GCTGATTGTC AATGAGGCTG CACCAAGCTC CGAGACTGAT 
GAAGGTGTTT TGGACACTGC TGCTGTGAAT GCGACGACAA CGCAACCTCC TGAGACTTCA 
GAAGACAACA AGCCACATGG CTTTAGTGCT GAGAATCAAG AAGTTGAAGA AGGCAGAGAA 
CCTATGCTAG CAGAGTTTGT AGACAGCTCA CAGCAGCTAG AGTTGAGGTC CCTTCCTTCT 
AGCCAGGTTA ACGTAATGCA GACTACAGAG CCATATGTCG GTGATGACAA GAGCAGTGGA 
AATGAAGATT CCATGGAGCA AAAATCACAG GACATTGAGC AGAAAAGTAA GGAACCAGAA 
GAGGAAGATT TACCCAAGGT GAAGTTAAAA TCTCCAGAAG ATGAAAGACG AGAGGAACTG 
GTTAAAGACA TAGTTGCCAA GGATAAATCT CTGATGAATT GTCTCCAGCC GATTTCTGTC 
AGAGAATCTG CTATGGATCT CATGAAAAGC CTCTTCCCTA TGGACTTCAC TGAAGCGGAA 
AAATCTAGAA CTAGAGGACT TCTTAGAAAA GACAACAGTG AAACATTAAG GAAAAACAAT 
TCAGACCTTG AAAGTTCTTC TAAACTTCCT TCAAAAATAA CCGTGATGCT TCAGAAGAGT 
TCTGCGCGGA GGCCAGATGG GGAAAGCCTA GATGACATCA CTTCAAAGAA GATGGAACTT 
CTTTCTAACA TCGGTTCCAA GCTAGAGGAC CTTTGTGAGC AAAAAGAAAT CCTTTTGGCT 
GATAATAGTA AGAACACGAC GCACGGCAAT AATATTCAGG CACTAGTGAA AGAGTTGTGT 
AAGCCCAATG AGTTTGAGCG CTACATGATG TTCATTGGGG ATCTGGAGAA AGTGGTGAGC 
CTGCTATTTT CCCTGTCGAC AAGGCTGACT CGGGTTGAAA ATGCTTTAAG CAAGGTTGAT 
GAAAATACAG ATGCAGAAGA GATGCAATCC CTAAAGGAGC GCCACAATCT CCTGTCCAGT 
CAAAGAGAAG ATGCCAAAGA CTTAAAGGCA AACTTAGACC GCAGAGAACA AGTTGTCACG 
GGAATCCTTG TAAAATACCT GAATGAGGAG CAGCTGCAGG ACTACAAGCA CTTTGTCAGA 
CTGAAGACCT CCCTCCTAAT TGAACAAAAG AACCTGGAGG AGAAGATAAA ACTGTACGAG 
GAGCAGTTAG AGAGCATCCA CGATAGCATC CCACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shroom1 ( XM_004912832.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912889.2 Xenopus tropicalis shroom family member 1 (shroom1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa54485
Clone ID Related Accession (Same CDS sequence)	XM_004912831.4 , XM_004912832.4 , XM_002935260.5
Accession Version	XM_004912832.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein Shroom1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004912832.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCAT TTGGTAATGC AATTGAGAGA TGGAACATAA AAAGTACAGG AGTCGTTGCA 
GGTCTGGGAC ATTCTGAAAG AATATCCCCC ATAAGATCTA TGACCACATT GGTGGATTCG 
GCCTATAGCT CATTTTCTGG CAGTTCGTAT GTTCCAGAAT ACCAAAACTC ATTTCAGCAT 
GACAGCTGTC ACTTTAATGA TGAACAGCTT TCTTACATGG ACTCTGAATA TGTAAGAGCA 
ATCTATAATC CTAGTGTAGT AGACATGGAT GGTGTATATC ATGATATATT GTCAGGACAT 
AGATGCTCGA ATGTAGCACT ATCTGGAAGA ACTAGCAGTG CCAGCTGTGA CCACCCATCA 
CAAGTGCATC AAACTTCACC AGCAAAACTG GATAACTATC TTACTAACCT TGGTTCTGAC 
CAGTTCAGTG GGACTTATGG GGAATCCATA AATATGAAGC ATAAGCAGAA TCACTCTAAT 
AGTATATATA TGCCTAACCA CAAAGCATAT GGCCTCCAAC GTAACAGTCC TACAGGAGTA 
AGCAGTTTGC AGGAAAAAGA AAACCAAATG TATAATCAAT CAAATTATAT GGAAATAAAA 
CATTATTTTA GTAGATCCTT AGATGTTCTT CAAACAGAAG GAGAGCTCAA GACACAAACC 
TCATGTACTC AGAATCTGCC GGATGAGGTC AGCTGCAAGT TCAGAAACAC ACATTCTACA 
AATAGAACGA GCAAAGACCT TTCCAAGGGA AACGATCTAC AAAAAAGCAA TGAAGAAAAC 
ACAGAGAGAG ACAGCCCATA TCTGACAAAA GAGGGTCCAG TTTCTCAAGG TCAATATGCA 
TCAGATGTAA GGACAAGTTT TAAAAACATA AGACGCTCCC TTAAAAAGTC TGTGTCTGAA 
AAAATTGGTG CTCATGACAA TAAAGGGGGT TGCTGGATTA TGAAGCCAGG TCAAGACCCT 
CATTGTTATA ATTCTGAGGG AACAGTTAAG GACGCAGAGT ATGATGACGT CGAGCAGTGG 
GATATCAGGA AATCAAGACT TAATACAAGG GCAAGCCAGA CTTTATATTG TGAGTCTAAT 
AAAGATGCCT CTTGCCCTCC CTTGAATGCT TTGAATTCTA AGAATGAAGT TCTCGAAGCT 
GATAATACAC TCTGTTTTCA AAAGGATGCA ACTGTAAAGT CTATTCCCCT ATTATCTCAG 
CATATTCAAC AGGAAAAATC TAAAAGCCAT CCCCTGTCTG ATTTAAACTG TGAGAAGATT 
ACCAAGGCAA CAACACCTAT GCTGTATCAT CTTGCTGGAG GAAGGCATAG TGCCTTTATA 
GCCCCTGTAC ACAATACAAA TCCAGCTAAA CAGGAAAAAC TCAAGCTTGA GTCAAAGACA 
TTTGAAAGAA CGAATAACTT CTCTATACTA CAACAAACCG AGCCAAGACC AGATAACCAT 
AAATTCCCCA AGAATAAAAG CCTAACACAA CTTGCTGATC TCCATGACTC TGCAGAGGGT 
GGGAATTCTG GGAATTTAAA TTCTTCAGCA GAAGAGTGCC TAATGAATGA CTACATTGAG 
AAACTGAAGG TTGCCCAGAA GAAAGTATTG AGGGAAACCT CTTTTAAAAG AAAGGACTTG 
CAGATGAGTT TGCCTTGCAG ATTCAAGTTG AATCCACCCA AAAGGCCTAC CATTGATCAT 
TTTCGATCCT ATTCTTCATC CAGTGCAAAT GAGGAGACGG CATTTCTCCA AACAAAAAAC 
TCTGTTGACA GCACCTACAA CAAAGATGAT GCTGAAAAGG TCGCAGTTAC TCGAATAGGA 
GGTCGGAAAC GGATTACAAA GGAACAAAAG AAGCTGTGTT ATTCAGAACC AGAGAAATTA 
GATCACCTTG GGATTCAAAA ATCTAACTTT GCATGGAAGG AGGAACCTGC TATCGCAAAT 
AAAAGGGAGA TGAGTGATTC TGATGTTGCT GCAAATAAGA TAAAGTATCT GGAGAGCAAA 
GAACGAACTG CCTCATCTTC AAACCTTTCT AAGACAGAAC TCAAACAGAT CCAGCATAAT 
GCGCTTGTTG AATATATGGA AAGGAAAACA AACCAAAGGC CCAGCAGCAA TCCACAATTC 
CAGATGGAAA GGACATCGGG ATTGCCAAAT TATAGTGAGT GGAATATCTA CTCATCTGAA 
ACCAGTTCTG GTGATGGTTC ACAAAAGTAC CTACGGAGGA GATCAGCTGG AGCTTCATCT 
TCCTATGATG CCACAGTAAC ATGGAATGAT AGATTTGGAA AGACTTCACC TCTTGGGCGG 
CGCTCTGCTG TGGAACCAAC TGCTGGTGTG CAACGCAGGA GTTTTTTTGA TCAACGCACT 
TTAGATGGCA GTCAAGAACG TCTTGAAGGT TCTTCCCCAT CTCTCAGTCA AAAAACATCA 
AAGTCAAGTC ATCGTGAACA GGTCTCGTAT GTAAATATGG AATTTCTTCC CTCCGCACTT 
TCCAAGAATC ACATTTATAA TGACCGGCTG ACTGTACCCG GGGACAACAC CAGCGCCGAC 
ACTGAGAGAA TTCGTGCTTC CAAAAGCAGA GGGAAGTCTA TGGAAGAAAT AGGGACTGCA 
GACATTGTAA CGCTTGCAGA GCTGAGCCAT AGCTCAGATC AACTTTACCA CATTAAAGGG 
CCTGTGATAT CGCCAAGGCT GGAAAACACA AGGACTGCAG CTGTGTCCCA TCAAGACAAC 
GTCTTGGCTA GCACTCAGAA CGAAACAGGA AATCTGCCCA GACAAACACA TCAAGAAAGT 
GTTCTGGGGC CCCGCAGATC AGACTTAGCA AATTTGGGAC AAGAGGCACA TTCCTGGCCC 
TTGAGAGCTA GTGCTGCTTC CTCAGGCATA GATAATCCCT GCTCTTCTCC TCCTTCGGCA 
AAGGTTCACC CCTGGGCTCC TGAGCCATTG CATTGCTTGG ACACAGAAGA TGAGGTCTTT 
ACACAAGCCA GCACTGCCAG AAATGAAGAT CCCAGCTCCA CTGCCCTTAG CCATCTGCAC 
GATATTGGAA ACCCTGTGTG TGAAGAGGAA GATACTGCAT TATGCTTTTC TTTCTTACCT 
GAGCAGGGTC ATCTAGAACG GCTGATTGTC AATGAGGCTG CACCAAGCTC CGAGACTGAT 
GAAGGTGTTT TGGACACTGC TGCTGTGAAT GCGACGACAA CGCAACCTCC TGAGACTTCA 
GAAGACAACA AGCCACATGG CTTTAGTGCT GAGAATCAAG AAGTTGAAGA AGGCAGAGAA 
CCTATGCTAG CAGAGTTTGT AGACAGCTCA CAGCAGCTAG AGTTGAGGTC CCTTCCTTCT 
AGCCAGGTTA ACGTAATGCA GACTACAGAG CCATATGTCG GTGATGACAA GAGCAGTGGA 
AATGAAGATT CCATGGAGCA AAAATCACAG GACATTGAGC AGAAAAGTAA GGAACCAGAA 
GAGGAAGATT TACCCAAGGT GAAGTTAAAA TCTCCAGAAG ATGAAAGACG AGAGGAACTG 
GTTAAAGACA TAGTTGCCAA GGATAAATCT CTGATGAATT GTCTCCAGCC GATTTCTGTC 
AGAGAATCTG CTATGGATCT CATGAAAAGC CTCTTCCCTA TGGACTTCAC TGAAGCGGAA 
AAATCTAGAA CTAGAGGACT TCTTAGAAAA GACAACAGTG AAACATTAAG GAAAAACAAT 
TCAGACCTTG AAAGTTCTTC TAAACTTCCT TCAAAAATAA CCGTGATGCT TCAGAAGAGT 
TCTGCGCGGA GGCCAGATGG GGAAAGCCTA GATGACATCA CTTCAAAGAA GATGGAACTT 
CTTTCTAACA TCGGTTCCAA GCTAGAGGAC CTTTGTGAGC AAAAAGAAAT CCTTTTGGCT 
GATAATAGTA AGAACACGAC GCACGGCAAT AATATTCAGG CACTAGTGAA AGAGTTGTGT 
AAGCCCAATG AGTTTGAGCG CTACATGATG TTCATTGGGG ATCTGGAGAA AGTGGTGAGC 
CTGCTATTTT CCCTGTCGAC AAGGCTGACT CGGGTTGAAA ATGCTTTAAG CAAGGTTGAT 
GAAAATACAG ATGCAGAAGA GATGCAATCC CTAAAGGAGC GCCACAATCT CCTGTCCAGT 
CAAAGAGAAG ATGCCAAAGA CTTAAAGGCA AACTTAGACC GCAGAGAACA AGTTGTCACG 
GGAATCCTTG TAAAATACCT GAATGAGGAG CAGCTGCAGG ACTACAAGCA CTTTGTCAGA 
CTGAAGACCT CCCTCCTAAT TGAACAAAAG AACCTGGAGG AGAAGATAAA ACTGTACGAG 
GAGCAGTTAG AGAGCATCCA CGATAGCATC CCACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912889.2
CDS	224..4522
Translation	MSSFGNAIERWNIKSTGVVAGLGHSERISPIRSMTTLVDSAYSSFSGSSYVPEYQNSFQHDSCHFNDEQLSYMDSEYVRAIYNPSVVDMDGVYHDILSGHRCSNVALSGRTSSASCDHPSQVHQTSPAKLDNYLTNLGSDQFSGTYGESINMKHKQNHSNSIYMPNHKAYGLQRNSPTGVSSLQEKENQMYNQSNYMEIKHYFSRSLDVLQTEGELKTQTSCTQNLPDEVSCKFRNTHSTNRTSKDLSKGNDLQKSNEENTERDSPYLTKEGPVSQGQYASDVRTSFKNIRRSLKKSVSEKIGAHDNKGGCWIMKPGQDPHCYNSEGTVKDAEYDDVEQWDIRKSRLNTRASQTLYCESNKDASCPPLNALNSKNEVLEADNTLCFQKDATVKSIPLLSQHIQQEKSKSHPLSDLNCEKITKATTPMLYHLAGGRHSAFIAPVHNTNPAKQEKLKLESKTFERTNNFSILQQTEPRPDNHKFPKNKSLTQLADLHDSAEGGNSGNLNSSAEECLMNDYIEKLKVAQKKVLRETSFKRKDLQMSLPCRFKLNPPKRPTIDHFRSYSSSSANEETAFLQTKNSVDSTYNKDDAEKVAVTRIGGRKRITKEQKKLCYSEPEKLDHLGIQKSNFAWKEEPAIANKREMSDSDVAANKIKYLESKERTASSSNLSKTELKQIQHNALVEYMERKTNQRPSSNPQFQMERTSGLPNYSEWNIYSSETSSGDGSQKYLRRRSAGASSSYDATVTWNDRFGKTSPLGRRSAVEPTAGVQRRSFFDQRTLDGSQERLEGSSPSLSQKTSKSSHREQVSYVNMEFLPSALSKNHIYNDRLTVPGDNTSADTERIRASKSRGKSMEEIGTADIVTLAELSHSSDQLYHIKGPVISPRLENTRTAAVSHQDNVLASTQNETGNLPRQTHQESVLGPRRSDLANLGQEAHSWPLRASAASSGIDNPCSSPPSAKVHPWAPEPLHCLDTEDEVFTQASTARNEDPSSTALSHLHDIGNPVCEEEDTALCFSFLPEQGHLERLIVNEAAPSSETDEGVLDTAAVNATTTQPPETSEDNKPHGFSAENQEVEEGREPMLAEFVDSSQQLELRSLPSSQVNVMQTTEPYVGDDKSSGNEDSMEQKSQDIEQKSKEPEEEDLPKVKLKSPEDERREELVKDIVAKDKSLMNCLQPISVRESAMDLMKSLFPMDFTEAEKSRTRGLLRKDNSETLRKNNSDLESSSKLPSKITVMLQKSSARRPDGESLDDITSKKMELLSNIGSKLEDLCEQKEILLADNSKNTTHGNNIQALVKELCKPNEFERYMMFIGDLEKVVSLLFSLSTRLTRVENALSKVDENTDAEEMQSLKERHNLLSSQREDAKDLKANLDRREQVVTGILVKYLNEEQLQDYKHFVRLKTSLLIEQKNLEEKIKLYEEQLESIHDSIPP

Target ORF information:

RefSeq Version	XM_004912832.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis shroom family member 1 (shroom1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912832.4

ORF Insert Sequence:

ATGTCATCAT TTGGTAATGC AATTGAGAGA TGGAACATAA AAAGTACAGG AGTCGTTGCA 
GGTCTGGGAC ATTCTGAAAG AATATCCCCC ATAAGATCTA TGACCACATT GGTGGATTCG 
GCCTATAGCT CATTTTCTGG CAGTTCGTAT GTTCCAGAAT ACCAAAACTC ATTTCAGCAT 
GACAGCTGTC ACTTTAATGA TGAACAGCTT TCTTACATGG ACTCTGAATA TGTAAGAGCA 
ATCTATAATC CTAGTGTAGT AGACATGGAT GGTGTATATC ATGATATATT GTCAGGACAT 
AGATGCTCGA ATGTAGCACT ATCTGGAAGA ACTAGCAGTG CCAGCTGTGA CCACCCATCA 
CAAGTGCATC AAACTTCACC AGCAAAACTG GATAACTATC TTACTAACCT TGGTTCTGAC 
CAGTTCAGTG GGACTTATGG GGAATCCATA AATATGAAGC ATAAGCAGAA TCACTCTAAT 
AGTATATATA TGCCTAACCA CAAAGCATAT GGCCTCCAAC GTAACAGTCC TACAGGAGTA 
AGCAGTTTGC AGGAAAAAGA AAACCAAATG TATAATCAAT CAAATTATAT GGAAATAAAA 
CATTATTTTA GTAGATCCTT AGATGTTCTT CAAACAGAAG GAGAGCTCAA GACACAAACC 
TCATGTACTC AGAATCTGCC GGATGAGGTC AGCTGCAAGT TCAGAAACAC ACATTCTACA 
AATAGAACGA GCAAAGACCT TTCCAAGGGA AACGATCTAC AAAAAAGCAA TGAAGAAAAC 
ACAGAGAGAG ACAGCCCATA TCTGACAAAA GAGGGTCCAG TTTCTCAAGG TCAATATGCA 
TCAGATGTAA GGACAAGTTT TAAAAACATA AGACGCTCCC TTAAAAAGTC TGTGTCTGAA 
AAAATTGGTG CTCATGACAA TAAAGGGGGT TGCTGGATTA TGAAGCCAGG TCAAGACCCT 
CATTGTTATA ATTCTGAGGG AACAGTTAAG GACGCAGAGT ATGATGACGT CGAGCAGTGG 
GATATCAGGA AATCAAGACT TAATACAAGG GCAAGCCAGA CTTTATATTG TGAGTCTAAT 
AAAGATGCCT CTTGCCCTCC CTTGAATGCT TTGAATTCTA AGAATGAAGT TCTCGAAGCT 
GATAATACAC TCTGTTTTCA AAAGGATGCA ACTGTAAAGT CTATTCCCCT ATTATCTCAG 
CATATTCAAC AGGAAAAATC TAAAAGCCAT CCCCTGTCTG ATTTAAACTG TGAGAAGATT 
ACCAAGGCAA CAACACCTAT GCTGTATCAT CTTGCTGGAG GAAGGCATAG TGCCTTTATA 
GCCCCTGTAC ACAATACAAA TCCAGCTAAA CAGGAAAAAC TCAAGCTTGA GTCAAAGACA 
TTTGAAAGAA CGAATAACTT CTCTATACTA CAACAAACCG AGCCAAGACC AGATAACCAT 
AAATTCCCCA AGAATAAAAG CCTAACACAA CTTGCTGATC TCCATGACTC TGCAGAGGGT 
GGGAATTCTG GGAATTTAAA TTCTTCAGCA GAAGAGTGCC TAATGAATGA CTACATTGAG 
AAACTGAAGG TTGCCCAGAA GAAAGTATTG AGGGAAACCT CTTTTAAAAG AAAGGACTTG 
CAGATGAGTT TGCCTTGCAG ATTCAAGTTG AATCCACCCA AAAGGCCTAC CATTGATCAT 
TTTCGATCCT ATTCTTCATC CAGTGCAAAT GAGGAGACGG CATTTCTCCA AACAAAAAAC 
TCTGTTGACA GCACCTACAA CAAAGATGAT GCTGAAAAGG TCGCAGTTAC TCGAATAGGA 
GGTCGGAAAC GGATTACAAA GGAACAAAAG AAGCTGTGTT ATTCAGAACC AGAGAAATTA 
GATCACCTTG GGATTCAAAA ATCTAACTTT GCATGGAAGG AGGAACCTGC TATCGCAAAT 
AAAAGGGAGA TGAGTGATTC TGATGTTGCT GCAAATAAGA TAAAGTATCT GGAGAGCAAA 
GAACGAACTG CCTCATCTTC AAACCTTTCT AAGACAGAAC TCAAACAGAT CCAGCATAAT 
GCGCTTGTTG AATATATGGA AAGGAAAACA AACCAAAGGC CCAGCAGCAA TCCACAATTC 
CAGATGGAAA GGACATCGGG ATTGCCAAAT TATAGTGAGT GGAATATCTA CTCATCTGAA 
ACCAGTTCTG GTGATGGTTC ACAAAAGTAC CTACGGAGGA GATCAGCTGG AGCTTCATCT 
TCCTATGATG CCACAGTAAC ATGGAATGAT AGATTTGGAA AGACTTCACC TCTTGGGCGG 
CGCTCTGCTG TGGAACCAAC TGCTGGTGTG CAACGCAGGA GTTTTTTTGA TCAACGCACT 
TTAGATGGCA GTCAAGAACG TCTTGAAGGT TCTTCCCCAT CTCTCAGTCA AAAAACATCA 
AAGTCAAGTC ATCGTGAACA GGTCTCGTAT GTAAATATGG AATTTCTTCC CTCCGCACTT 
TCCAAGAATC ACATTTATAA TGACCGGCTG ACTGTACCCG GGGACAACAC CAGCGCCGAC 
ACTGAGAGAA TTCGTGCTTC CAAAAGCAGA GGGAAGTCTA TGGAAGAAAT AGGGACTGCA 
GACATTGTAA CGCTTGCAGA GCTGAGCCAT AGCTCAGATC AACTTTACCA CATTAAAGGG 
CCTGTGATAT CGCCAAGGCT GGAAAACACA AGGACTGCAG CTGTGTCCCA TCAAGACAAC 
GTCTTGGCTA GCACTCAGAA CGAAACAGGA AATCTGCCCA GACAAACACA TCAAGAAAGT 
GTTCTGGGGC CCCGCAGATC AGACTTAGCA AATTTGGGAC AAGAGGCACA TTCCTGGCCC 
TTGAGAGCTA GTGCTGCTTC CTCAGGCATA GATAATCCCT GCTCTTCTCC TCCTTCGGCA 
AAGGTTCACC CCTGGGCTCC TGAGCCATTG CATTGCTTGG ACACAGAAGA TGAGGTCTTT 
ACACAAGCCA GCACTGCCAG AAATGAAGAT CCCAGCTCCA CTGCCCTTAG CCATCTGCAC 
GATATTGGAA ACCCTGTGTG TGAAGAGGAA GATACTGCAT TATGCTTTTC TTTCTTACCT 
GAGCAGGGTC ATCTAGAACG GCTGATTGTC AATGAGGCTG CACCAAGCTC CGAGACTGAT 
GAAGGTGTTT TGGACACTGC TGCTGTGAAT GCGACGACAA CGCAACCTCC TGAGACTTCA 
GAAGACAACA AGCCACATGG CTTTAGTGCT GAGAATCAAG AAGTTGAAGA AGGCAGAGAA 
CCTATGCTAG CAGAGTTTGT AGACAGCTCA CAGCAGCTAG AGTTGAGGTC CCTTCCTTCT 
AGCCAGGTTA ACGTAATGCA GACTACAGAG CCATATGTCG GTGATGACAA GAGCAGTGGA 
AATGAAGATT CCATGGAGCA AAAATCACAG GACATTGAGC AGAAAAGTAA GGAACCAGAA 
GAGGAAGATT TACCCAAGGT GAAGTTAAAA TCTCCAGAAG ATGAAAGACG AGAGGAACTG 
GTTAAAGACA TAGTTGCCAA GGATAAATCT CTGATGAATT GTCTCCAGCC GATTTCTGTC 
AGAGAATCTG CTATGGATCT CATGAAAAGC CTCTTCCCTA TGGACTTCAC TGAAGCGGAA 
AAATCTAGAA CTAGAGGACT TCTTAGAAAA GACAACAGTG AAACATTAAG GAAAAACAAT 
TCAGACCTTG AAAGTTCTTC TAAACTTCCT TCAAAAATAA CCGTGATGCT TCAGAAGAGT 
TCTGCGCGGA GGCCAGATGG GGAAAGCCTA GATGACATCA CTTCAAAGAA GATGGAACTT 
CTTTCTAACA TCGGTTCCAA GCTAGAGGAC CTTTGTGAGC AAAAAGAAAT CCTTTTGGCT 
GATAATAGTA AGAACACGAC GCACGGCAAT AATATTCAGG CACTAGTGAA AGAGTTGTGT 
AAGCCCAATG AGTTTGAGCG CTACATGATG TTCATTGGGG ATCTGGAGAA AGTGGTGAGC 
CTGCTATTTT CCCTGTCGAC AAGGCTGACT CGGGTTGAAA ATGCTTTAAG CAAGGTTGAT 
GAAAATACAG ATGCAGAAGA GATGCAATCC CTAAAGGAGC GCCACAATCT CCTGTCCAGT 
CAAAGAGAAG ATGCCAAAGA CTTAAAGGCA AACTTAGACC GCAGAGAACA AGTTGTCACG 
GGAATCCTTG TAAAATACCT GAATGAGGAG CAGCTGCAGG ACTACAAGCA CTTTGTCAGA 
CTGAAGACCT CCCTCCTAAT TGAACAAAAG AACCTGGAGG AGAAGATAAA ACTGTACGAG 
GAGCAGTTAG AGAGCATCCA CGATAGCATC CCACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shroom1 ( XM_002935260.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935306.3 Xenopus tropicalis shroom family member 1 (shroom1), transcript variant X1, mRNA.

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CloneID	OXa54485
Clone ID Related Accession (Same CDS sequence)	XM_004912831.4 , XM_004912832.4 , XM_002935260.5
Accession Version	XM_002935260.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein Shroom1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002935260.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCAT TTGGTAATGC AATTGAGAGA TGGAACATAA AAAGTACAGG AGTCGTTGCA 
GGTCTGGGAC ATTCTGAAAG AATATCCCCC ATAAGATCTA TGACCACATT GGTGGATTCG 
GCCTATAGCT CATTTTCTGG CAGTTCGTAT GTTCCAGAAT ACCAAAACTC ATTTCAGCAT 
GACAGCTGTC ACTTTAATGA TGAACAGCTT TCTTACATGG ACTCTGAATA TGTAAGAGCA 
ATCTATAATC CTAGTGTAGT AGACATGGAT GGTGTATATC ATGATATATT GTCAGGACAT 
AGATGCTCGA ATGTAGCACT ATCTGGAAGA ACTAGCAGTG CCAGCTGTGA CCACCCATCA 
CAAGTGCATC AAACTTCACC AGCAAAACTG GATAACTATC TTACTAACCT TGGTTCTGAC 
CAGTTCAGTG GGACTTATGG GGAATCCATA AATATGAAGC ATAAGCAGAA TCACTCTAAT 
AGTATATATA TGCCTAACCA CAAAGCATAT GGCCTCCAAC GTAACAGTCC TACAGGAGTA 
AGCAGTTTGC AGGAAAAAGA AAACCAAATG TATAATCAAT CAAATTATAT GGAAATAAAA 
CATTATTTTA GTAGATCCTT AGATGTTCTT CAAACAGAAG GAGAGCTCAA GACACAAACC 
TCATGTACTC AGAATCTGCC GGATGAGGTC AGCTGCAAGT TCAGAAACAC ACATTCTACA 
AATAGAACGA GCAAAGACCT TTCCAAGGGA AACGATCTAC AAAAAAGCAA TGAAGAAAAC 
ACAGAGAGAG ACAGCCCATA TCTGACAAAA GAGGGTCCAG TTTCTCAAGG TCAATATGCA 
TCAGATGTAA GGACAAGTTT TAAAAACATA AGACGCTCCC TTAAAAAGTC TGTGTCTGAA 
AAAATTGGTG CTCATGACAA TAAAGGGGGT TGCTGGATTA TGAAGCCAGG TCAAGACCCT 
CATTGTTATA ATTCTGAGGG AACAGTTAAG GACGCAGAGT ATGATGACGT CGAGCAGTGG 
GATATCAGGA AATCAAGACT TAATACAAGG GCAAGCCAGA CTTTATATTG TGAGTCTAAT 
AAAGATGCCT CTTGCCCTCC CTTGAATGCT TTGAATTCTA AGAATGAAGT TCTCGAAGCT 
GATAATACAC TCTGTTTTCA AAAGGATGCA ACTGTAAAGT CTATTCCCCT ATTATCTCAG 
CATATTCAAC AGGAAAAATC TAAAAGCCAT CCCCTGTCTG ATTTAAACTG TGAGAAGATT 
ACCAAGGCAA CAACACCTAT GCTGTATCAT CTTGCTGGAG GAAGGCATAG TGCCTTTATA 
GCCCCTGTAC ACAATACAAA TCCAGCTAAA CAGGAAAAAC TCAAGCTTGA GTCAAAGACA 
TTTGAAAGAA CGAATAACTT CTCTATACTA CAACAAACCG AGCCAAGACC AGATAACCAT 
AAATTCCCCA AGAATAAAAG CCTAACACAA CTTGCTGATC TCCATGACTC TGCAGAGGGT 
GGGAATTCTG GGAATTTAAA TTCTTCAGCA GAAGAGTGCC TAATGAATGA CTACATTGAG 
AAACTGAAGG TTGCCCAGAA GAAAGTATTG AGGGAAACCT CTTTTAAAAG AAAGGACTTG 
CAGATGAGTT TGCCTTGCAG ATTCAAGTTG AATCCACCCA AAAGGCCTAC CATTGATCAT 
TTTCGATCCT ATTCTTCATC CAGTGCAAAT GAGGAGACGG CATTTCTCCA AACAAAAAAC 
TCTGTTGACA GCACCTACAA CAAAGATGAT GCTGAAAAGG TCGCAGTTAC TCGAATAGGA 
GGTCGGAAAC GGATTACAAA GGAACAAAAG AAGCTGTGTT ATTCAGAACC AGAGAAATTA 
GATCACCTTG GGATTCAAAA ATCTAACTTT GCATGGAAGG AGGAACCTGC TATCGCAAAT 
AAAAGGGAGA TGAGTGATTC TGATGTTGCT GCAAATAAGA TAAAGTATCT GGAGAGCAAA 
GAACGAACTG CCTCATCTTC AAACCTTTCT AAGACAGAAC TCAAACAGAT CCAGCATAAT 
GCGCTTGTTG AATATATGGA AAGGAAAACA AACCAAAGGC CCAGCAGCAA TCCACAATTC 
CAGATGGAAA GGACATCGGG ATTGCCAAAT TATAGTGAGT GGAATATCTA CTCATCTGAA 
ACCAGTTCTG GTGATGGTTC ACAAAAGTAC CTACGGAGGA GATCAGCTGG AGCTTCATCT 
TCCTATGATG CCACAGTAAC ATGGAATGAT AGATTTGGAA AGACTTCACC TCTTGGGCGG 
CGCTCTGCTG TGGAACCAAC TGCTGGTGTG CAACGCAGGA GTTTTTTTGA TCAACGCACT 
TTAGATGGCA GTCAAGAACG TCTTGAAGGT TCTTCCCCAT CTCTCAGTCA AAAAACATCA 
AAGTCAAGTC ATCGTGAACA GGTCTCGTAT GTAAATATGG AATTTCTTCC CTCCGCACTT 
TCCAAGAATC ACATTTATAA TGACCGGCTG ACTGTACCCG GGGACAACAC CAGCGCCGAC 
ACTGAGAGAA TTCGTGCTTC CAAAAGCAGA GGGAAGTCTA TGGAAGAAAT AGGGACTGCA 
GACATTGTAA CGCTTGCAGA GCTGAGCCAT AGCTCAGATC AACTTTACCA CATTAAAGGG 
CCTGTGATAT CGCCAAGGCT GGAAAACACA AGGACTGCAG CTGTGTCCCA TCAAGACAAC 
GTCTTGGCTA GCACTCAGAA CGAAACAGGA AATCTGCCCA GACAAACACA TCAAGAAAGT 
GTTCTGGGGC CCCGCAGATC AGACTTAGCA AATTTGGGAC AAGAGGCACA TTCCTGGCCC 
TTGAGAGCTA GTGCTGCTTC CTCAGGCATA GATAATCCCT GCTCTTCTCC TCCTTCGGCA 
AAGGTTCACC CCTGGGCTCC TGAGCCATTG CATTGCTTGG ACACAGAAGA TGAGGTCTTT 
ACACAAGCCA GCACTGCCAG AAATGAAGAT CCCAGCTCCA CTGCCCTTAG CCATCTGCAC 
GATATTGGAA ACCCTGTGTG TGAAGAGGAA GATACTGCAT TATGCTTTTC TTTCTTACCT 
GAGCAGGGTC ATCTAGAACG GCTGATTGTC AATGAGGCTG CACCAAGCTC CGAGACTGAT 
GAAGGTGTTT TGGACACTGC TGCTGTGAAT GCGACGACAA CGCAACCTCC TGAGACTTCA 
GAAGACAACA AGCCACATGG CTTTAGTGCT GAGAATCAAG AAGTTGAAGA AGGCAGAGAA 
CCTATGCTAG CAGAGTTTGT AGACAGCTCA CAGCAGCTAG AGTTGAGGTC CCTTCCTTCT 
AGCCAGGTTA ACGTAATGCA GACTACAGAG CCATATGTCG GTGATGACAA GAGCAGTGGA 
AATGAAGATT CCATGGAGCA AAAATCACAG GACATTGAGC AGAAAAGTAA GGAACCAGAA 
GAGGAAGATT TACCCAAGGT GAAGTTAAAA TCTCCAGAAG ATGAAAGACG AGAGGAACTG 
GTTAAAGACA TAGTTGCCAA GGATAAATCT CTGATGAATT GTCTCCAGCC GATTTCTGTC 
AGAGAATCTG CTATGGATCT CATGAAAAGC CTCTTCCCTA TGGACTTCAC TGAAGCGGAA 
AAATCTAGAA CTAGAGGACT TCTTAGAAAA GACAACAGTG AAACATTAAG GAAAAACAAT 
TCAGACCTTG AAAGTTCTTC TAAACTTCCT TCAAAAATAA CCGTGATGCT TCAGAAGAGT 
TCTGCGCGGA GGCCAGATGG GGAAAGCCTA GATGACATCA CTTCAAAGAA GATGGAACTT 
CTTTCTAACA TCGGTTCCAA GCTAGAGGAC CTTTGTGAGC AAAAAGAAAT CCTTTTGGCT 
GATAATAGTA AGAACACGAC GCACGGCAAT AATATTCAGG CACTAGTGAA AGAGTTGTGT 
AAGCCCAATG AGTTTGAGCG CTACATGATG TTCATTGGGG ATCTGGAGAA AGTGGTGAGC 
CTGCTATTTT CCCTGTCGAC AAGGCTGACT CGGGTTGAAA ATGCTTTAAG CAAGGTTGAT 
GAAAATACAG ATGCAGAAGA GATGCAATCC CTAAAGGAGC GCCACAATCT CCTGTCCAGT 
CAAAGAGAAG ATGCCAAAGA CTTAAAGGCA AACTTAGACC GCAGAGAACA AGTTGTCACG 
GGAATCCTTG TAAAATACCT GAATGAGGAG CAGCTGCAGG ACTACAAGCA CTTTGTCAGA 
CTGAAGACCT CCCTCCTAAT TGAACAAAAG AACCTGGAGG AGAAGATAAA ACTGTACGAG 
GAGCAGTTAG AGAGCATCCA CGATAGCATC CCACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935306.3
CDS	325..4623
Translation	MSSFGNAIERWNIKSTGVVAGLGHSERISPIRSMTTLVDSAYSSFSGSSYVPEYQNSFQHDSCHFNDEQLSYMDSEYVRAIYNPSVVDMDGVYHDILSGHRCSNVALSGRTSSASCDHPSQVHQTSPAKLDNYLTNLGSDQFSGTYGESINMKHKQNHSNSIYMPNHKAYGLQRNSPTGVSSLQEKENQMYNQSNYMEIKHYFSRSLDVLQTEGELKTQTSCTQNLPDEVSCKFRNTHSTNRTSKDLSKGNDLQKSNEENTERDSPYLTKEGPVSQGQYASDVRTSFKNIRRSLKKSVSEKIGAHDNKGGCWIMKPGQDPHCYNSEGTVKDAEYDDVEQWDIRKSRLNTRASQTLYCESNKDASCPPLNALNSKNEVLEADNTLCFQKDATVKSIPLLSQHIQQEKSKSHPLSDLNCEKITKATTPMLYHLAGGRHSAFIAPVHNTNPAKQEKLKLESKTFERTNNFSILQQTEPRPDNHKFPKNKSLTQLADLHDSAEGGNSGNLNSSAEECLMNDYIEKLKVAQKKVLRETSFKRKDLQMSLPCRFKLNPPKRPTIDHFRSYSSSSANEETAFLQTKNSVDSTYNKDDAEKVAVTRIGGRKRITKEQKKLCYSEPEKLDHLGIQKSNFAWKEEPAIANKREMSDSDVAANKIKYLESKERTASSSNLSKTELKQIQHNALVEYMERKTNQRPSSNPQFQMERTSGLPNYSEWNIYSSETSSGDGSQKYLRRRSAGASSSYDATVTWNDRFGKTSPLGRRSAVEPTAGVQRRSFFDQRTLDGSQERLEGSSPSLSQKTSKSSHREQVSYVNMEFLPSALSKNHIYNDRLTVPGDNTSADTERIRASKSRGKSMEEIGTADIVTLAELSHSSDQLYHIKGPVISPRLENTRTAAVSHQDNVLASTQNETGNLPRQTHQESVLGPRRSDLANLGQEAHSWPLRASAASSGIDNPCSSPPSAKVHPWAPEPLHCLDTEDEVFTQASTARNEDPSSTALSHLHDIGNPVCEEEDTALCFSFLPEQGHLERLIVNEAAPSSETDEGVLDTAAVNATTTQPPETSEDNKPHGFSAENQEVEEGREPMLAEFVDSSQQLELRSLPSSQVNVMQTTEPYVGDDKSSGNEDSMEQKSQDIEQKSKEPEEEDLPKVKLKSPEDERREELVKDIVAKDKSLMNCLQPISVRESAMDLMKSLFPMDFTEAEKSRTRGLLRKDNSETLRKNNSDLESSSKLPSKITVMLQKSSARRPDGESLDDITSKKMELLSNIGSKLEDLCEQKEILLADNSKNTTHGNNIQALVKELCKPNEFERYMMFIGDLEKVVSLLFSLSTRLTRVENALSKVDENTDAEEMQSLKERHNLLSSQREDAKDLKANLDRREQVVTGILVKYLNEEQLQDYKHFVRLKTSLLIEQKNLEEKIKLYEEQLESIHDSIPP

Target ORF information:

RefSeq Version	XM_002935260.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis shroom family member 1 (shroom1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935260.5

ORF Insert Sequence:

ATGTCATCAT TTGGTAATGC AATTGAGAGA TGGAACATAA AAAGTACAGG AGTCGTTGCA 
GGTCTGGGAC ATTCTGAAAG AATATCCCCC ATAAGATCTA TGACCACATT GGTGGATTCG 
GCCTATAGCT CATTTTCTGG CAGTTCGTAT GTTCCAGAAT ACCAAAACTC ATTTCAGCAT 
GACAGCTGTC ACTTTAATGA TGAACAGCTT TCTTACATGG ACTCTGAATA TGTAAGAGCA 
ATCTATAATC CTAGTGTAGT AGACATGGAT GGTGTATATC ATGATATATT GTCAGGACAT 
AGATGCTCGA ATGTAGCACT ATCTGGAAGA ACTAGCAGTG CCAGCTGTGA CCACCCATCA 
CAAGTGCATC AAACTTCACC AGCAAAACTG GATAACTATC TTACTAACCT TGGTTCTGAC 
CAGTTCAGTG GGACTTATGG GGAATCCATA AATATGAAGC ATAAGCAGAA TCACTCTAAT 
AGTATATATA TGCCTAACCA CAAAGCATAT GGCCTCCAAC GTAACAGTCC TACAGGAGTA 
AGCAGTTTGC AGGAAAAAGA AAACCAAATG TATAATCAAT CAAATTATAT GGAAATAAAA 
CATTATTTTA GTAGATCCTT AGATGTTCTT CAAACAGAAG GAGAGCTCAA GACACAAACC 
TCATGTACTC AGAATCTGCC GGATGAGGTC AGCTGCAAGT TCAGAAACAC ACATTCTACA 
AATAGAACGA GCAAAGACCT TTCCAAGGGA AACGATCTAC AAAAAAGCAA TGAAGAAAAC 
ACAGAGAGAG ACAGCCCATA TCTGACAAAA GAGGGTCCAG TTTCTCAAGG TCAATATGCA 
TCAGATGTAA GGACAAGTTT TAAAAACATA AGACGCTCCC TTAAAAAGTC TGTGTCTGAA 
AAAATTGGTG CTCATGACAA TAAAGGGGGT TGCTGGATTA TGAAGCCAGG TCAAGACCCT 
CATTGTTATA ATTCTGAGGG AACAGTTAAG GACGCAGAGT ATGATGACGT CGAGCAGTGG 
GATATCAGGA AATCAAGACT TAATACAAGG GCAAGCCAGA CTTTATATTG TGAGTCTAAT 
AAAGATGCCT CTTGCCCTCC CTTGAATGCT TTGAATTCTA AGAATGAAGT TCTCGAAGCT 
GATAATACAC TCTGTTTTCA AAAGGATGCA ACTGTAAAGT CTATTCCCCT ATTATCTCAG 
CATATTCAAC AGGAAAAATC TAAAAGCCAT CCCCTGTCTG ATTTAAACTG TGAGAAGATT 
ACCAAGGCAA CAACACCTAT GCTGTATCAT CTTGCTGGAG GAAGGCATAG TGCCTTTATA 
GCCCCTGTAC ACAATACAAA TCCAGCTAAA CAGGAAAAAC TCAAGCTTGA GTCAAAGACA 
TTTGAAAGAA CGAATAACTT CTCTATACTA CAACAAACCG AGCCAAGACC AGATAACCAT 
AAATTCCCCA AGAATAAAAG CCTAACACAA CTTGCTGATC TCCATGACTC TGCAGAGGGT 
GGGAATTCTG GGAATTTAAA TTCTTCAGCA GAAGAGTGCC TAATGAATGA CTACATTGAG 
AAACTGAAGG TTGCCCAGAA GAAAGTATTG AGGGAAACCT CTTTTAAAAG AAAGGACTTG 
CAGATGAGTT TGCCTTGCAG ATTCAAGTTG AATCCACCCA AAAGGCCTAC CATTGATCAT 
TTTCGATCCT ATTCTTCATC CAGTGCAAAT GAGGAGACGG CATTTCTCCA AACAAAAAAC 
TCTGTTGACA GCACCTACAA CAAAGATGAT GCTGAAAAGG TCGCAGTTAC TCGAATAGGA 
GGTCGGAAAC GGATTACAAA GGAACAAAAG AAGCTGTGTT ATTCAGAACC AGAGAAATTA 
GATCACCTTG GGATTCAAAA ATCTAACTTT GCATGGAAGG AGGAACCTGC TATCGCAAAT 
AAAAGGGAGA TGAGTGATTC TGATGTTGCT GCAAATAAGA TAAAGTATCT GGAGAGCAAA 
GAACGAACTG CCTCATCTTC AAACCTTTCT AAGACAGAAC TCAAACAGAT CCAGCATAAT 
GCGCTTGTTG AATATATGGA AAGGAAAACA AACCAAAGGC CCAGCAGCAA TCCACAATTC 
CAGATGGAAA GGACATCGGG ATTGCCAAAT TATAGTGAGT GGAATATCTA CTCATCTGAA 
ACCAGTTCTG GTGATGGTTC ACAAAAGTAC CTACGGAGGA GATCAGCTGG AGCTTCATCT 
TCCTATGATG CCACAGTAAC ATGGAATGAT AGATTTGGAA AGACTTCACC TCTTGGGCGG 
CGCTCTGCTG TGGAACCAAC TGCTGGTGTG CAACGCAGGA GTTTTTTTGA TCAACGCACT 
TTAGATGGCA GTCAAGAACG TCTTGAAGGT TCTTCCCCAT CTCTCAGTCA AAAAACATCA 
AAGTCAAGTC ATCGTGAACA GGTCTCGTAT GTAAATATGG AATTTCTTCC CTCCGCACTT 
TCCAAGAATC ACATTTATAA TGACCGGCTG ACTGTACCCG GGGACAACAC CAGCGCCGAC 
ACTGAGAGAA TTCGTGCTTC CAAAAGCAGA GGGAAGTCTA TGGAAGAAAT AGGGACTGCA 
GACATTGTAA CGCTTGCAGA GCTGAGCCAT AGCTCAGATC AACTTTACCA CATTAAAGGG 
CCTGTGATAT CGCCAAGGCT GGAAAACACA AGGACTGCAG CTGTGTCCCA TCAAGACAAC 
GTCTTGGCTA GCACTCAGAA CGAAACAGGA AATCTGCCCA GACAAACACA TCAAGAAAGT 
GTTCTGGGGC CCCGCAGATC AGACTTAGCA AATTTGGGAC AAGAGGCACA TTCCTGGCCC 
TTGAGAGCTA GTGCTGCTTC CTCAGGCATA GATAATCCCT GCTCTTCTCC TCCTTCGGCA 
AAGGTTCACC CCTGGGCTCC TGAGCCATTG CATTGCTTGG ACACAGAAGA TGAGGTCTTT 
ACACAAGCCA GCACTGCCAG AAATGAAGAT CCCAGCTCCA CTGCCCTTAG CCATCTGCAC 
GATATTGGAA ACCCTGTGTG TGAAGAGGAA GATACTGCAT TATGCTTTTC TTTCTTACCT 
GAGCAGGGTC ATCTAGAACG GCTGATTGTC AATGAGGCTG CACCAAGCTC CGAGACTGAT 
GAAGGTGTTT TGGACACTGC TGCTGTGAAT GCGACGACAA CGCAACCTCC TGAGACTTCA 
GAAGACAACA AGCCACATGG CTTTAGTGCT GAGAATCAAG AAGTTGAAGA AGGCAGAGAA 
CCTATGCTAG CAGAGTTTGT AGACAGCTCA CAGCAGCTAG AGTTGAGGTC CCTTCCTTCT 
AGCCAGGTTA ACGTAATGCA GACTACAGAG CCATATGTCG GTGATGACAA GAGCAGTGGA 
AATGAAGATT CCATGGAGCA AAAATCACAG GACATTGAGC AGAAAAGTAA GGAACCAGAA 
GAGGAAGATT TACCCAAGGT GAAGTTAAAA TCTCCAGAAG ATGAAAGACG AGAGGAACTG 
GTTAAAGACA TAGTTGCCAA GGATAAATCT CTGATGAATT GTCTCCAGCC GATTTCTGTC 
AGAGAATCTG CTATGGATCT CATGAAAAGC CTCTTCCCTA TGGACTTCAC TGAAGCGGAA 
AAATCTAGAA CTAGAGGACT TCTTAGAAAA GACAACAGTG AAACATTAAG GAAAAACAAT 
TCAGACCTTG AAAGTTCTTC TAAACTTCCT TCAAAAATAA CCGTGATGCT TCAGAAGAGT 
TCTGCGCGGA GGCCAGATGG GGAAAGCCTA GATGACATCA CTTCAAAGAA GATGGAACTT 
CTTTCTAACA TCGGTTCCAA GCTAGAGGAC CTTTGTGAGC AAAAAGAAAT CCTTTTGGCT 
GATAATAGTA AGAACACGAC GCACGGCAAT AATATTCAGG CACTAGTGAA AGAGTTGTGT 
AAGCCCAATG AGTTTGAGCG CTACATGATG TTCATTGGGG ATCTGGAGAA AGTGGTGAGC 
CTGCTATTTT CCCTGTCGAC AAGGCTGACT CGGGTTGAAA ATGCTTTAAG CAAGGTTGAT 
GAAAATACAG ATGCAGAAGA GATGCAATCC CTAAAGGAGC GCCACAATCT CCTGTCCAGT 
CAAAGAGAAG ATGCCAAAGA CTTAAAGGCA AACTTAGACC GCAGAGAACA AGTTGTCACG 
GGAATCCTTG TAAAATACCT GAATGAGGAG CAGCTGCAGG ACTACAAGCA CTTTGTCAGA 
CTGAAGACCT CCCTCCTAAT TGAACAAAAG AACCTGGAGG AGAAGATAAA ACTGTACGAG 
GAGCAGTTAG AGAGCATCCA CGATAGCATC CCACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shroom1 ( XM_004912831.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912888.1 Xenopus tropicalis shroom family member 1 (shroom1), transcript variant X2, mRNA.

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CloneID	OXa07579
Clone ID Related Accession (Same CDS sequence)	XM_004912831.3 , XM_004912832.3 , XM_002935260.4
Accession Version	XM_004912831.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein Shroom1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004912831.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCAT TTGGTAATGC AATTGAGAGA TGGAACATAA AAAGTACAGG AGTCGTTGCA 
GGTCTGGGAC ATTCTGAAAG AATATCCCCC ATAAGATCTA TGACCACATT GGTGGATTCG 
GCCTATAGCT CATTTTCTGG CAGTTCGTAT GTTCCAGAAT ACCAAAACTC ATTTCAGCAT 
GACAGCTGTC ACTTTAATGA TGAACAGCTT TCTTACATGG ACTCTGAATA TGTAAGAGCA 
ATCTATAATC CTAGTGTAGT AGACATGGAT GGTGTATATC ATGATATATT GTCAGGACAT 
AGATGCTCGA ATGTAGCACT ATCTGGAAGA ACTAGCAGTG CCAGCTGTGA CCACCCATCA 
CAAGTGCATC AAACTTCACC AGCAAAACTG GATAACTATC TTACTAACCT TGGTTCTGAC 
CAGTTCAGTG GGACTTATGG GGAATCCATA AATATGAAGC ATAAGCAGAA TCACTCTAAT 
AGTATATATA TGCCTAACCA CAAAGCATAT GGCCTCCAAC GTAACAGTCC TACAGGAGTA 
AGCAGTTTGC AGGAAAAAGA AAACCAAATG TATAATCAAT CAAATTATAT GGAAATAAAA 
GATTATTTTA GTAGATCCTT AGATGTTCTT CAAACAGAAG GAGAGCTCAA GACACAAACC 
TCATGTACTC AGAATCTGCC GGATGAGGTC AGCTGCAAGT TCAGAAACAC ACATTCTACA 
AATAGAACGA GCAAAGACCT TTCCAAGGGA AACGATCTAC AAAAAAGCAA TGAAGAAAAC 
ACAGAGAGAG ACAGCCCATA TCTGACAAAA GAGGGTCCAG TTTCTCAAGG TCAATATGCA 
TCAGATGTAA GGACAAGTTT TAAAAACATA AGACGCTCCC TTAAAAAGTC TGTGTCTGAA 
AAAATTGGTG CTCATGACAA TAAAGGGGGT TGCTGGATTA TGAAGCCAGG TCAAGACCCT 
CATTGTTATA ATTCTGAGGG AACAGTTAAG GACGCAGAGT ATGATGACGT CGAGCAGTGG 
GATATCAGGA AATCAAGACT TAATACAAGG GCAAGCCAGA CTTTATATTG TGAGTCTAAT 
AAAGATGCCT CTTGCCCTCC CTTGAATGCT TTGAATTCTA AGAATGAAGT TCTCGAAGCT 
GATAATACAC TCTGTTTTCA AAAGGATGCA ACTGTAAAGT CTATTCCCCT ATTATCTCAG 
CATATTCAAC AGGAAAAATC TAAAAGCCAT CCCCTGTCTG ATTTAAACTG TGAGAAGATT 
ACCAAGGCAA CAACACCTAT GCTGTATCAT CTTGCTGGAG GAAGGCATAG TGCCTTTATA 
GCCCCTGTAC ACAATACAAA TCCAGCTAAA CAGGAAAAAC TCAAGCTTGA GTCAAAGACA 
TTTGAAAGAA CGAATAACTT CTCTATACTA CAACAAACCG AGCCAAGACC AGATAACCAT 
AAATTCCCCA AGAATAAAAG CCTAACACAA CTTGCTGATC TCCATGACTC TGCAGAGGGT 
GGGAATTCTG GGAATTTAAA TTCTTCAGCA GAAGAGTGCC TAATGAATGA CTACATTGAG 
AAACTGAAGG TTGCCCAGAA GAAAGTATTG AGGGAAACCT CTTTTAAAAG AAAGGACTTG 
CAGATGAGTT TGCCTTGCAG ATTCAAGTTG AATCCACCCA AAAGGCCTAC CATTGATCAT 
TTTCGATCCT ATTCTTCATC CAGTGCAAAT GAGGAGACGG CATTTCTCCA AACAAAAAAC 
TCTGTTGACA GCACCTACAA CAAAGATGAT GCTGAAAAGG TCGCAGTTAC TCGAATAGGA 
GGTCGGAAAC GGATTACAAA GGAACAAAAG AAGCTGTGTT ATTCAGAACC AGAGAAATTA 
GATCACCTTG GGATTCAAAA ATCTAACTTT GCATGGAAGG AGGAACCTGC TATCGCAAAT 
AAAAGGGAGA TGAGTGATTC TGATGTTGCT GCAAATAAGA TAAAGTATCT GGAGAGCAAA 
GAACGAACTG CCTCATCTTC AAACCTTTCT AAGACAGAAC TCAAACAGAT CCAGCATAAT 
GCGCTTGTTG AATATATGGA AAGGAAAACA AACCAAAGGC CCAGCAGCAA TCCACAATTC 
CAGATGGAAA GGACATCGGG ATTGCCAAAT TATAGTGAGT GGAATATCTA CTCATCTGAA 
ACCAGTTCTG GTGATGGTTC ACAAAAGTAC CTACGGAGGA GATCAGCTGG AGCTTCATCT 
TCCTATGATG CCACAGTAAC ATGGAATGAT AGATTTGGAA AGACTTCACC TCTTGGGCGG 
CGCTCTGCTG TGGAACCAAC TGCTGGTGTG CAACGCAGGA GTTTTTTTGA TCAACGCACT 
TTAGATGGCA GTCAAGAACG TCTTGAAGGT TCTTCCCCAT CTCTCAGTCA AAAAACATCA 
AAGTCAAGTC ATCGTGAACA GGTCTCGTAT GTAAATATGG AATTTCTTCC CTCAGCACTT 
TCCAAGAATC ACATTTATAA TGACCGGCTG ACTGTACCCG GGGACAACAC CAGCGCCGAC 
ACTGAGAGAA TTCGTGCTTC CAAAAGCAGA GGGAAGTCTA TGGAAGAAAT AGGGACTGCA 
GACATTGTAA CGCTTGCAGA GCTGAGCCAT AGCTCAGATC AACTTTACCA CATTAAAGGG 
CCTGTGATAT CGCCAAGGCT GGAAAACACA AGGACTGCAG CTGTGTCCCA TCAAGACAAC 
GTCTTGGCTA GCACTCAGAA CGAAACAGGA AATCTGCCCA GACAAACACA TCAAGAAAGT 
GTTCTGGGGC CCCGCAGATC AGATTTAGCA AATTTGGGAC AAGAGGCACA TTCCTGGCCC 
TTGAGAGCTA GTGCTGCTTC CTCAGGCATA GATAATTCCT GCTCTTCTCC TCCTTCGGCA 
AAGGTTCACC CCTGGGCTCC TGAGCCATTG CATTGCTTGG ACACAGAAGA TGAGGTCTTT 
ACACAAGCCA GCACTGCCAG AAATGAAGAT CCCAGCTCCA CTGCCCTTAG CCATCTGCAC 
GATATTGGAA ACCCTGTGTG TGAAGAGGAA GATACTGCAT TATGCTTTTC TTTCTTACCT 
GAGCAGGGTC ATCTAGAACG GCTGATTGTC AATGAGGCTG CACCAAGCTC CAAGACTGAT 
GAAGGTGTTT TGGACACTGC TGCTGAGAAT GCGACGACAA CGCAACCTCC TGAGACTTCA 
GAAGACAACA AGCCACATGG CTTTAGTGCT GAGAATCAAG AAGTTGAAGA AGGCAGAGAA 
CCTATGCTAG CAGAGTTTGT AGACAGCTCA CAGCAGCTAG AGTTGAGGTC CCTTCCTTCT 
AGCCAGGTTA ACGTAATGCA GACTACAGAG CCATATGTCG GTGATGACAA GAGCAGTGGA 
AATGAAGATT CCATGGAGCA AAAATCACAG GACATTGAGC AGAAAAGTAA GGAACCAGAA 
GAGGAAGATT TACCCAAGGT GAAGTTAAAA TCTCCAGAAG ATGAAAGACG AGAGGAACTG 
GTTAAAGACA TAGTTGCCAA GGATAAATCT CTGATGAATT GTCTCCAGCC GATTTCTGTC 
AGAGAATCTG CTATGGATCT CATGAAAAGC CTCTTCCCTA TGGACTTCAC TGAAGCGGAA 
AAATCTAGAA CTAGAGGACT TCTTAGAAAA GACAACAGTG AAACATTAAG GAAAAACAAT 
TCAGACCTTG AAAGTTCTTC TAAACTTCCT TCAAAAATAA CCGTGATGCT TCAGAAGAGT 
TCTGCGCGTA GGCCAGATGG GGAAAGCCTA GATGACATCA CTTCAAAGAA GATGGAACTT 
CTTTCTAACA TCGGTTCCAA GCTAGAGGAC CTTTGTGAGC AGAAAGAAAT CCTTTTGGCT 
GATAATAGTA AGAACACGAC GCACGGCAAT AATATTCAGG CGCTAGTGAA AGAGTTGTGT 
AAGCCCAATG AGTTTGAGCG CTACATGATG TTCATTGGGG ATCTGGAGAA AGTGGTGAGC 
CTGCTATTTT CCCTGTCGAC AAGGCTGACT CGGGTTGAAA ATGCTTTAAG CAAGGTTGAT 
GAAAATACAG ATGCAGAAGA GATGCAATCC CTAAAGGAGC GCCACAATCT CCTGTCCAGT 
CAAAGAGAAG ATGCCAAAGA CTTAAAGGCA AACTTAGACC GCAGAGAACA AGTTGTCACG 
GGAATCCTTG TAAAATACCT GAATGAGGAG CAGCTGCAGG ACTACAAGCA CTTTGTCAGA 
CTGAAGACCT CCCTCCTAAT TGAACAAAAG AACCTGGAGG AGAAGATAAA ACTGTACGAG 
GAGCAGTTAG AGAGCATCCA CGATAGCATC CCACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912888.1
CDS	400..4698
Translation	MSSFGNAIERWNIKSTGVVAGLGHSERISPIRSMTTLVDSAYSSFSGSSYVPEYQNSFQHDSCHFNDEQLSYMDSEYVRAIYNPSVVDMDGVYHDILSGHRCSNVALSGRTSSASCDHPSQVHQTSPAKLDNYLTNLGSDQFSGTYGESINMKHKQNHSNSIYMPNHKAYGLQRNSPTGVSSLQEKENQMYNQSNYMEIKDYFSRSLDVLQTEGELKTQTSCTQNLPDEVSCKFRNTHSTNRTSKDLSKGNDLQKSNEENTERDSPYLTKEGPVSQGQYASDVRTSFKNIRRSLKKSVSEKIGAHDNKGGCWIMKPGQDPHCYNSEGTVKDAEYDDVEQWDIRKSRLNTRASQTLYCESNKDASCPPLNALNSKNEVLEADNTLCFQKDATVKSIPLLSQHIQQEKSKSHPLSDLNCEKITKATTPMLYHLAGGRHSAFIAPVHNTNPAKQEKLKLESKTFERTNNFSILQQTEPRPDNHKFPKNKSLTQLADLHDSAEGGNSGNLNSSAEECLMNDYIEKLKVAQKKVLRETSFKRKDLQMSLPCRFKLNPPKRPTIDHFRSYSSSSANEETAFLQTKNSVDSTYNKDDAEKVAVTRIGGRKRITKEQKKLCYSEPEKLDHLGIQKSNFAWKEEPAIANKREMSDSDVAANKIKYLESKERTASSSNLSKTELKQIQHNALVEYMERKTNQRPSSNPQFQMERTSGLPNYSEWNIYSSETSSGDGSQKYLRRRSAGASSSYDATVTWNDRFGKTSPLGRRSAVEPTAGVQRRSFFDQRTLDGSQERLEGSSPSLSQKTSKSSHREQVSYVNMEFLPSALSKNHIYNDRLTVPGDNTSADTERIRASKSRGKSMEEIGTADIVTLAELSHSSDQLYHIKGPVISPRLENTRTAAVSHQDNVLASTQNETGNLPRQTHQESVLGPRRSDLANLGQEAHSWPLRASAASSGIDNSCSSPPSAKVHPWAPEPLHCLDTEDEVFTQASTARNEDPSSTALSHLHDIGNPVCEEEDTALCFSFLPEQGHLERLIVNEAAPSSKTDEGVLDTAAENATTTQPPETSEDNKPHGFSAENQEVEEGREPMLAEFVDSSQQLELRSLPSSQVNVMQTTEPYVGDDKSSGNEDSMEQKSQDIEQKSKEPEEEDLPKVKLKSPEDERREELVKDIVAKDKSLMNCLQPISVRESAMDLMKSLFPMDFTEAEKSRTRGLLRKDNSETLRKNNSDLESSSKLPSKITVMLQKSSARRPDGESLDDITSKKMELLSNIGSKLEDLCEQKEILLADNSKNTTHGNNIQALVKELCKPNEFERYMMFIGDLEKVVSLLFSLSTRLTRVENALSKVDENTDAEEMQSLKERHNLLSSQREDAKDLKANLDRREQVVTGILVKYLNEEQLQDYKHFVRLKTSLLIEQKNLEEKIKLYEEQLESIHDSIPP

Target ORF information:

RefSeq Version	XM_004912831.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis shroom family member 1 (shroom1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912831.3

ORF Insert Sequence:

ATGTCATCAT TTGGTAATGC AATTGAGAGA TGGAACATAA AAAGTACAGG AGTCGTTGCA 
GGTCTGGGAC ATTCTGAAAG AATATCCCCC ATAAGATCTA TGACCACATT GGTGGATTCG 
GCCTATAGCT CATTTTCTGG CAGTTCGTAT GTTCCAGAAT ACCAAAACTC ATTTCAGCAT 
GACAGCTGTC ACTTTAATGA TGAACAGCTT TCTTACATGG ACTCTGAATA TGTAAGAGCA 
ATCTATAATC CTAGTGTAGT AGACATGGAT GGTGTATATC ATGATATATT GTCAGGACAT 
AGATGCTCGA ATGTAGCACT ATCTGGAAGA ACTAGCAGTG CCAGCTGTGA CCACCCATCA 
CAAGTGCATC AAACTTCACC AGCAAAACTG GATAACTATC TTACTAACCT TGGTTCTGAC 
CAGTTCAGTG GGACTTATGG GGAATCCATA AATATGAAGC ATAAGCAGAA TCACTCTAAT 
AGTATATATA TGCCTAACCA CAAAGCATAT GGCCTCCAAC GTAACAGTCC TACAGGAGTA 
AGCAGTTTGC AGGAAAAAGA AAACCAAATG TATAATCAAT CAAATTATAT GGAAATAAAA 
GATTATTTTA GTAGATCCTT AGATGTTCTT CAAACAGAAG GAGAGCTCAA GACACAAACC 
TCATGTACTC AGAATCTGCC GGATGAGGTC AGCTGCAAGT TCAGAAACAC ACATTCTACA 
AATAGAACGA GCAAAGACCT TTCCAAGGGA AACGATCTAC AAAAAAGCAA TGAAGAAAAC 
ACAGAGAGAG ACAGCCCATA TCTGACAAAA GAGGGTCCAG TTTCTCAAGG TCAATATGCA 
TCAGATGTAA GGACAAGTTT TAAAAACATA AGACGCTCCC TTAAAAAGTC TGTGTCTGAA 
AAAATTGGTG CTCATGACAA TAAAGGGGGT TGCTGGATTA TGAAGCCAGG TCAAGACCCT 
CATTGTTATA ATTCTGAGGG AACAGTTAAG GACGCAGAGT ATGATGACGT CGAGCAGTGG 
GATATCAGGA AATCAAGACT TAATACAAGG GCAAGCCAGA CTTTATATTG TGAGTCTAAT 
AAAGATGCCT CTTGCCCTCC CTTGAATGCT TTGAATTCTA AGAATGAAGT TCTCGAAGCT 
GATAATACAC TCTGTTTTCA AAAGGATGCA ACTGTAAAGT CTATTCCCCT ATTATCTCAG 
CATATTCAAC AGGAAAAATC TAAAAGCCAT CCCCTGTCTG ATTTAAACTG TGAGAAGATT 
ACCAAGGCAA CAACACCTAT GCTGTATCAT CTTGCTGGAG GAAGGCATAG TGCCTTTATA 
GCCCCTGTAC ACAATACAAA TCCAGCTAAA CAGGAAAAAC TCAAGCTTGA GTCAAAGACA 
TTTGAAAGAA CGAATAACTT CTCTATACTA CAACAAACCG AGCCAAGACC AGATAACCAT 
AAATTCCCCA AGAATAAAAG CCTAACACAA CTTGCTGATC TCCATGACTC TGCAGAGGGT 
GGGAATTCTG GGAATTTAAA TTCTTCAGCA GAAGAGTGCC TAATGAATGA CTACATTGAG 
AAACTGAAGG TTGCCCAGAA GAAAGTATTG AGGGAAACCT CTTTTAAAAG AAAGGACTTG 
CAGATGAGTT TGCCTTGCAG ATTCAAGTTG AATCCACCCA AAAGGCCTAC CATTGATCAT 
TTTCGATCCT ATTCTTCATC CAGTGCAAAT GAGGAGACGG CATTTCTCCA AACAAAAAAC 
TCTGTTGACA GCACCTACAA CAAAGATGAT GCTGAAAAGG TCGCAGTTAC TCGAATAGGA 
GGTCGGAAAC GGATTACAAA GGAACAAAAG AAGCTGTGTT ATTCAGAACC AGAGAAATTA 
GATCACCTTG GGATTCAAAA ATCTAACTTT GCATGGAAGG AGGAACCTGC TATCGCAAAT 
AAAAGGGAGA TGAGTGATTC TGATGTTGCT GCAAATAAGA TAAAGTATCT GGAGAGCAAA 
GAACGAACTG CCTCATCTTC AAACCTTTCT AAGACAGAAC TCAAACAGAT CCAGCATAAT 
GCGCTTGTTG AATATATGGA AAGGAAAACA AACCAAAGGC CCAGCAGCAA TCCACAATTC 
CAGATGGAAA GGACATCGGG ATTGCCAAAT TATAGTGAGT GGAATATCTA CTCATCTGAA 
ACCAGTTCTG GTGATGGTTC ACAAAAGTAC CTACGGAGGA GATCAGCTGG AGCTTCATCT 
TCCTATGATG CCACAGTAAC ATGGAATGAT AGATTTGGAA AGACTTCACC TCTTGGGCGG 
CGCTCTGCTG TGGAACCAAC TGCTGGTGTG CAACGCAGGA GTTTTTTTGA TCAACGCACT 
TTAGATGGCA GTCAAGAACG TCTTGAAGGT TCTTCCCCAT CTCTCAGTCA AAAAACATCA 
AAGTCAAGTC ATCGTGAACA GGTCTCGTAT GTAAATATGG AATTTCTTCC CTCAGCACTT 
TCCAAGAATC ACATTTATAA TGACCGGCTG ACTGTACCCG GGGACAACAC CAGCGCCGAC 
ACTGAGAGAA TTCGTGCTTC CAAAAGCAGA GGGAAGTCTA TGGAAGAAAT AGGGACTGCA 
GACATTGTAA CGCTTGCAGA GCTGAGCCAT AGCTCAGATC AACTTTACCA CATTAAAGGG 
CCTGTGATAT CGCCAAGGCT GGAAAACACA AGGACTGCAG CTGTGTCCCA TCAAGACAAC 
GTCTTGGCTA GCACTCAGAA CGAAACAGGA AATCTGCCCA GACAAACACA TCAAGAAAGT 
GTTCTGGGGC CCCGCAGATC AGATTTAGCA AATTTGGGAC AAGAGGCACA TTCCTGGCCC 
TTGAGAGCTA GTGCTGCTTC CTCAGGCATA GATAATTCCT GCTCTTCTCC TCCTTCGGCA 
AAGGTTCACC CCTGGGCTCC TGAGCCATTG CATTGCTTGG ACACAGAAGA TGAGGTCTTT 
ACACAAGCCA GCACTGCCAG AAATGAAGAT CCCAGCTCCA CTGCCCTTAG CCATCTGCAC 
GATATTGGAA ACCCTGTGTG TGAAGAGGAA GATACTGCAT TATGCTTTTC TTTCTTACCT 
GAGCAGGGTC ATCTAGAACG GCTGATTGTC AATGAGGCTG CACCAAGCTC CAAGACTGAT 
GAAGGTGTTT TGGACACTGC TGCTGAGAAT GCGACGACAA CGCAACCTCC TGAGACTTCA 
GAAGACAACA AGCCACATGG CTTTAGTGCT GAGAATCAAG AAGTTGAAGA AGGCAGAGAA 
CCTATGCTAG CAGAGTTTGT AGACAGCTCA CAGCAGCTAG AGTTGAGGTC CCTTCCTTCT 
AGCCAGGTTA ACGTAATGCA GACTACAGAG CCATATGTCG GTGATGACAA GAGCAGTGGA 
AATGAAGATT CCATGGAGCA AAAATCACAG GACATTGAGC AGAAAAGTAA GGAACCAGAA 
GAGGAAGATT TACCCAAGGT GAAGTTAAAA TCTCCAGAAG ATGAAAGACG AGAGGAACTG 
GTTAAAGACA TAGTTGCCAA GGATAAATCT CTGATGAATT GTCTCCAGCC GATTTCTGTC 
AGAGAATCTG CTATGGATCT CATGAAAAGC CTCTTCCCTA TGGACTTCAC TGAAGCGGAA 
AAATCTAGAA CTAGAGGACT TCTTAGAAAA GACAACAGTG AAACATTAAG GAAAAACAAT 
TCAGACCTTG AAAGTTCTTC TAAACTTCCT TCAAAAATAA CCGTGATGCT TCAGAAGAGT 
TCTGCGCGTA GGCCAGATGG GGAAAGCCTA GATGACATCA CTTCAAAGAA GATGGAACTT 
CTTTCTAACA TCGGTTCCAA GCTAGAGGAC CTTTGTGAGC AGAAAGAAAT CCTTTTGGCT 
GATAATAGTA AGAACACGAC GCACGGCAAT AATATTCAGG CGCTAGTGAA AGAGTTGTGT 
AAGCCCAATG AGTTTGAGCG CTACATGATG TTCATTGGGG ATCTGGAGAA AGTGGTGAGC 
CTGCTATTTT CCCTGTCGAC AAGGCTGACT CGGGTTGAAA ATGCTTTAAG CAAGGTTGAT 
GAAAATACAG ATGCAGAAGA GATGCAATCC CTAAAGGAGC GCCACAATCT CCTGTCCAGT 
CAAAGAGAAG ATGCCAAAGA CTTAAAGGCA AACTTAGACC GCAGAGAACA AGTTGTCACG 
GGAATCCTTG TAAAATACCT GAATGAGGAG CAGCTGCAGG ACTACAAGCA CTTTGTCAGA 
CTGAAGACCT CCCTCCTAAT TGAACAAAAG AACCTGGAGG AGAAGATAAA ACTGTACGAG 
GAGCAGTTAG AGAGCATCCA CGATAGCATC CCACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shroom1 ( XM_004912832.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912889.1 Xenopus tropicalis shroom family member 1 (shroom1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa07579
Clone ID Related Accession (Same CDS sequence)	XM_004912831.3 , XM_004912832.3 , XM_002935260.4
Accession Version	XM_004912832.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein Shroom1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004912832.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCAT TTGGTAATGC AATTGAGAGA TGGAACATAA AAAGTACAGG AGTCGTTGCA 
GGTCTGGGAC ATTCTGAAAG AATATCCCCC ATAAGATCTA TGACCACATT GGTGGATTCG 
GCCTATAGCT CATTTTCTGG CAGTTCGTAT GTTCCAGAAT ACCAAAACTC ATTTCAGCAT 
GACAGCTGTC ACTTTAATGA TGAACAGCTT TCTTACATGG ACTCTGAATA TGTAAGAGCA 
ATCTATAATC CTAGTGTAGT AGACATGGAT GGTGTATATC ATGATATATT GTCAGGACAT 
AGATGCTCGA ATGTAGCACT ATCTGGAAGA ACTAGCAGTG CCAGCTGTGA CCACCCATCA 
CAAGTGCATC AAACTTCACC AGCAAAACTG GATAACTATC TTACTAACCT TGGTTCTGAC 
CAGTTCAGTG GGACTTATGG GGAATCCATA AATATGAAGC ATAAGCAGAA TCACTCTAAT 
AGTATATATA TGCCTAACCA CAAAGCATAT GGCCTCCAAC GTAACAGTCC TACAGGAGTA 
AGCAGTTTGC AGGAAAAAGA AAACCAAATG TATAATCAAT CAAATTATAT GGAAATAAAA 
GATTATTTTA GTAGATCCTT AGATGTTCTT CAAACAGAAG GAGAGCTCAA GACACAAACC 
TCATGTACTC AGAATCTGCC GGATGAGGTC AGCTGCAAGT TCAGAAACAC ACATTCTACA 
AATAGAACGA GCAAAGACCT TTCCAAGGGA AACGATCTAC AAAAAAGCAA TGAAGAAAAC 
ACAGAGAGAG ACAGCCCATA TCTGACAAAA GAGGGTCCAG TTTCTCAAGG TCAATATGCA 
TCAGATGTAA GGACAAGTTT TAAAAACATA AGACGCTCCC TTAAAAAGTC TGTGTCTGAA 
AAAATTGGTG CTCATGACAA TAAAGGGGGT TGCTGGATTA TGAAGCCAGG TCAAGACCCT 
CATTGTTATA ATTCTGAGGG AACAGTTAAG GACGCAGAGT ATGATGACGT CGAGCAGTGG 
GATATCAGGA AATCAAGACT TAATACAAGG GCAAGCCAGA CTTTATATTG TGAGTCTAAT 
AAAGATGCCT CTTGCCCTCC CTTGAATGCT TTGAATTCTA AGAATGAAGT TCTCGAAGCT 
GATAATACAC TCTGTTTTCA AAAGGATGCA ACTGTAAAGT CTATTCCCCT ATTATCTCAG 
CATATTCAAC AGGAAAAATC TAAAAGCCAT CCCCTGTCTG ATTTAAACTG TGAGAAGATT 
ACCAAGGCAA CAACACCTAT GCTGTATCAT CTTGCTGGAG GAAGGCATAG TGCCTTTATA 
GCCCCTGTAC ACAATACAAA TCCAGCTAAA CAGGAAAAAC TCAAGCTTGA GTCAAAGACA 
TTTGAAAGAA CGAATAACTT CTCTATACTA CAACAAACCG AGCCAAGACC AGATAACCAT 
AAATTCCCCA AGAATAAAAG CCTAACACAA CTTGCTGATC TCCATGACTC TGCAGAGGGT 
GGGAATTCTG GGAATTTAAA TTCTTCAGCA GAAGAGTGCC TAATGAATGA CTACATTGAG 
AAACTGAAGG TTGCCCAGAA GAAAGTATTG AGGGAAACCT CTTTTAAAAG AAAGGACTTG 
CAGATGAGTT TGCCTTGCAG ATTCAAGTTG AATCCACCCA AAAGGCCTAC CATTGATCAT 
TTTCGATCCT ATTCTTCATC CAGTGCAAAT GAGGAGACGG CATTTCTCCA AACAAAAAAC 
TCTGTTGACA GCACCTACAA CAAAGATGAT GCTGAAAAGG TCGCAGTTAC TCGAATAGGA 
GGTCGGAAAC GGATTACAAA GGAACAAAAG AAGCTGTGTT ATTCAGAACC AGAGAAATTA 
GATCACCTTG GGATTCAAAA ATCTAACTTT GCATGGAAGG AGGAACCTGC TATCGCAAAT 
AAAAGGGAGA TGAGTGATTC TGATGTTGCT GCAAATAAGA TAAAGTATCT GGAGAGCAAA 
GAACGAACTG CCTCATCTTC AAACCTTTCT AAGACAGAAC TCAAACAGAT CCAGCATAAT 
GCGCTTGTTG AATATATGGA AAGGAAAACA AACCAAAGGC CCAGCAGCAA TCCACAATTC 
CAGATGGAAA GGACATCGGG ATTGCCAAAT TATAGTGAGT GGAATATCTA CTCATCTGAA 
ACCAGTTCTG GTGATGGTTC ACAAAAGTAC CTACGGAGGA GATCAGCTGG AGCTTCATCT 
TCCTATGATG CCACAGTAAC ATGGAATGAT AGATTTGGAA AGACTTCACC TCTTGGGCGG 
CGCTCTGCTG TGGAACCAAC TGCTGGTGTG CAACGCAGGA GTTTTTTTGA TCAACGCACT 
TTAGATGGCA GTCAAGAACG TCTTGAAGGT TCTTCCCCAT CTCTCAGTCA AAAAACATCA 
AAGTCAAGTC ATCGTGAACA GGTCTCGTAT GTAAATATGG AATTTCTTCC CTCAGCACTT 
TCCAAGAATC ACATTTATAA TGACCGGCTG ACTGTACCCG GGGACAACAC CAGCGCCGAC 
ACTGAGAGAA TTCGTGCTTC CAAAAGCAGA GGGAAGTCTA TGGAAGAAAT AGGGACTGCA 
GACATTGTAA CGCTTGCAGA GCTGAGCCAT AGCTCAGATC AACTTTACCA CATTAAAGGG 
CCTGTGATAT CGCCAAGGCT GGAAAACACA AGGACTGCAG CTGTGTCCCA TCAAGACAAC 
GTCTTGGCTA GCACTCAGAA CGAAACAGGA AATCTGCCCA GACAAACACA TCAAGAAAGT 
GTTCTGGGGC CCCGCAGATC AGATTTAGCA AATTTGGGAC AAGAGGCACA TTCCTGGCCC 
TTGAGAGCTA GTGCTGCTTC CTCAGGCATA GATAATTCCT GCTCTTCTCC TCCTTCGGCA 
AAGGTTCACC CCTGGGCTCC TGAGCCATTG CATTGCTTGG ACACAGAAGA TGAGGTCTTT 
ACACAAGCCA GCACTGCCAG AAATGAAGAT CCCAGCTCCA CTGCCCTTAG CCATCTGCAC 
GATATTGGAA ACCCTGTGTG TGAAGAGGAA GATACTGCAT TATGCTTTTC TTTCTTACCT 
GAGCAGGGTC ATCTAGAACG GCTGATTGTC AATGAGGCTG CACCAAGCTC CAAGACTGAT 
GAAGGTGTTT TGGACACTGC TGCTGAGAAT GCGACGACAA CGCAACCTCC TGAGACTTCA 
GAAGACAACA AGCCACATGG CTTTAGTGCT GAGAATCAAG AAGTTGAAGA AGGCAGAGAA 
CCTATGCTAG CAGAGTTTGT AGACAGCTCA CAGCAGCTAG AGTTGAGGTC CCTTCCTTCT 
AGCCAGGTTA ACGTAATGCA GACTACAGAG CCATATGTCG GTGATGACAA GAGCAGTGGA 
AATGAAGATT CCATGGAGCA AAAATCACAG GACATTGAGC AGAAAAGTAA GGAACCAGAA 
GAGGAAGATT TACCCAAGGT GAAGTTAAAA TCTCCAGAAG ATGAAAGACG AGAGGAACTG 
GTTAAAGACA TAGTTGCCAA GGATAAATCT CTGATGAATT GTCTCCAGCC GATTTCTGTC 
AGAGAATCTG CTATGGATCT CATGAAAAGC CTCTTCCCTA TGGACTTCAC TGAAGCGGAA 
AAATCTAGAA CTAGAGGACT TCTTAGAAAA GACAACAGTG AAACATTAAG GAAAAACAAT 
TCAGACCTTG AAAGTTCTTC TAAACTTCCT TCAAAAATAA CCGTGATGCT TCAGAAGAGT 
TCTGCGCGTA GGCCAGATGG GGAAAGCCTA GATGACATCA CTTCAAAGAA GATGGAACTT 
CTTTCTAACA TCGGTTCCAA GCTAGAGGAC CTTTGTGAGC AGAAAGAAAT CCTTTTGGCT 
GATAATAGTA AGAACACGAC GCACGGCAAT AATATTCAGG CGCTAGTGAA AGAGTTGTGT 
AAGCCCAATG AGTTTGAGCG CTACATGATG TTCATTGGGG ATCTGGAGAA AGTGGTGAGC 
CTGCTATTTT CCCTGTCGAC AAGGCTGACT CGGGTTGAAA ATGCTTTAAG CAAGGTTGAT 
GAAAATACAG ATGCAGAAGA GATGCAATCC CTAAAGGAGC GCCACAATCT CCTGTCCAGT 
CAAAGAGAAG ATGCCAAAGA CTTAAAGGCA AACTTAGACC GCAGAGAACA AGTTGTCACG 
GGAATCCTTG TAAAATACCT GAATGAGGAG CAGCTGCAGG ACTACAAGCA CTTTGTCAGA 
CTGAAGACCT CCCTCCTAAT TGAACAAAAG AACCTGGAGG AGAAGATAAA ACTGTACGAG 
GAGCAGTTAG AGAGCATCCA CGATAGCATC CCACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912889.1
CDS	228..4526
Translation	MSSFGNAIERWNIKSTGVVAGLGHSERISPIRSMTTLVDSAYSSFSGSSYVPEYQNSFQHDSCHFNDEQLSYMDSEYVRAIYNPSVVDMDGVYHDILSGHRCSNVALSGRTSSASCDHPSQVHQTSPAKLDNYLTNLGSDQFSGTYGESINMKHKQNHSNSIYMPNHKAYGLQRNSPTGVSSLQEKENQMYNQSNYMEIKDYFSRSLDVLQTEGELKTQTSCTQNLPDEVSCKFRNTHSTNRTSKDLSKGNDLQKSNEENTERDSPYLTKEGPVSQGQYASDVRTSFKNIRRSLKKSVSEKIGAHDNKGGCWIMKPGQDPHCYNSEGTVKDAEYDDVEQWDIRKSRLNTRASQTLYCESNKDASCPPLNALNSKNEVLEADNTLCFQKDATVKSIPLLSQHIQQEKSKSHPLSDLNCEKITKATTPMLYHLAGGRHSAFIAPVHNTNPAKQEKLKLESKTFERTNNFSILQQTEPRPDNHKFPKNKSLTQLADLHDSAEGGNSGNLNSSAEECLMNDYIEKLKVAQKKVLRETSFKRKDLQMSLPCRFKLNPPKRPTIDHFRSYSSSSANEETAFLQTKNSVDSTYNKDDAEKVAVTRIGGRKRITKEQKKLCYSEPEKLDHLGIQKSNFAWKEEPAIANKREMSDSDVAANKIKYLESKERTASSSNLSKTELKQIQHNALVEYMERKTNQRPSSNPQFQMERTSGLPNYSEWNIYSSETSSGDGSQKYLRRRSAGASSSYDATVTWNDRFGKTSPLGRRSAVEPTAGVQRRSFFDQRTLDGSQERLEGSSPSLSQKTSKSSHREQVSYVNMEFLPSALSKNHIYNDRLTVPGDNTSADTERIRASKSRGKSMEEIGTADIVTLAELSHSSDQLYHIKGPVISPRLENTRTAAVSHQDNVLASTQNETGNLPRQTHQESVLGPRRSDLANLGQEAHSWPLRASAASSGIDNSCSSPPSAKVHPWAPEPLHCLDTEDEVFTQASTARNEDPSSTALSHLHDIGNPVCEEEDTALCFSFLPEQGHLERLIVNEAAPSSKTDEGVLDTAAENATTTQPPETSEDNKPHGFSAENQEVEEGREPMLAEFVDSSQQLELRSLPSSQVNVMQTTEPYVGDDKSSGNEDSMEQKSQDIEQKSKEPEEEDLPKVKLKSPEDERREELVKDIVAKDKSLMNCLQPISVRESAMDLMKSLFPMDFTEAEKSRTRGLLRKDNSETLRKNNSDLESSSKLPSKITVMLQKSSARRPDGESLDDITSKKMELLSNIGSKLEDLCEQKEILLADNSKNTTHGNNIQALVKELCKPNEFERYMMFIGDLEKVVSLLFSLSTRLTRVENALSKVDENTDAEEMQSLKERHNLLSSQREDAKDLKANLDRREQVVTGILVKYLNEEQLQDYKHFVRLKTSLLIEQKNLEEKIKLYEEQLESIHDSIPP

Target ORF information:

RefSeq Version	XM_004912832.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis shroom family member 1 (shroom1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912832.3

ORF Insert Sequence:

ATGTCATCAT TTGGTAATGC AATTGAGAGA TGGAACATAA AAAGTACAGG AGTCGTTGCA 
GGTCTGGGAC ATTCTGAAAG AATATCCCCC ATAAGATCTA TGACCACATT GGTGGATTCG 
GCCTATAGCT CATTTTCTGG CAGTTCGTAT GTTCCAGAAT ACCAAAACTC ATTTCAGCAT 
GACAGCTGTC ACTTTAATGA TGAACAGCTT TCTTACATGG ACTCTGAATA TGTAAGAGCA 
ATCTATAATC CTAGTGTAGT AGACATGGAT GGTGTATATC ATGATATATT GTCAGGACAT 
AGATGCTCGA ATGTAGCACT ATCTGGAAGA ACTAGCAGTG CCAGCTGTGA CCACCCATCA 
CAAGTGCATC AAACTTCACC AGCAAAACTG GATAACTATC TTACTAACCT TGGTTCTGAC 
CAGTTCAGTG GGACTTATGG GGAATCCATA AATATGAAGC ATAAGCAGAA TCACTCTAAT 
AGTATATATA TGCCTAACCA CAAAGCATAT GGCCTCCAAC GTAACAGTCC TACAGGAGTA 
AGCAGTTTGC AGGAAAAAGA AAACCAAATG TATAATCAAT CAAATTATAT GGAAATAAAA 
GATTATTTTA GTAGATCCTT AGATGTTCTT CAAACAGAAG GAGAGCTCAA GACACAAACC 
TCATGTACTC AGAATCTGCC GGATGAGGTC AGCTGCAAGT TCAGAAACAC ACATTCTACA 
AATAGAACGA GCAAAGACCT TTCCAAGGGA AACGATCTAC AAAAAAGCAA TGAAGAAAAC 
ACAGAGAGAG ACAGCCCATA TCTGACAAAA GAGGGTCCAG TTTCTCAAGG TCAATATGCA 
TCAGATGTAA GGACAAGTTT TAAAAACATA AGACGCTCCC TTAAAAAGTC TGTGTCTGAA 
AAAATTGGTG CTCATGACAA TAAAGGGGGT TGCTGGATTA TGAAGCCAGG TCAAGACCCT 
CATTGTTATA ATTCTGAGGG AACAGTTAAG GACGCAGAGT ATGATGACGT CGAGCAGTGG 
GATATCAGGA AATCAAGACT TAATACAAGG GCAAGCCAGA CTTTATATTG TGAGTCTAAT 
AAAGATGCCT CTTGCCCTCC CTTGAATGCT TTGAATTCTA AGAATGAAGT TCTCGAAGCT 
GATAATACAC TCTGTTTTCA AAAGGATGCA ACTGTAAAGT CTATTCCCCT ATTATCTCAG 
CATATTCAAC AGGAAAAATC TAAAAGCCAT CCCCTGTCTG ATTTAAACTG TGAGAAGATT 
ACCAAGGCAA CAACACCTAT GCTGTATCAT CTTGCTGGAG GAAGGCATAG TGCCTTTATA 
GCCCCTGTAC ACAATACAAA TCCAGCTAAA CAGGAAAAAC TCAAGCTTGA GTCAAAGACA 
TTTGAAAGAA CGAATAACTT CTCTATACTA CAACAAACCG AGCCAAGACC AGATAACCAT 
AAATTCCCCA AGAATAAAAG CCTAACACAA CTTGCTGATC TCCATGACTC TGCAGAGGGT 
GGGAATTCTG GGAATTTAAA TTCTTCAGCA GAAGAGTGCC TAATGAATGA CTACATTGAG 
AAACTGAAGG TTGCCCAGAA GAAAGTATTG AGGGAAACCT CTTTTAAAAG AAAGGACTTG 
CAGATGAGTT TGCCTTGCAG ATTCAAGTTG AATCCACCCA AAAGGCCTAC CATTGATCAT 
TTTCGATCCT ATTCTTCATC CAGTGCAAAT GAGGAGACGG CATTTCTCCA AACAAAAAAC 
TCTGTTGACA GCACCTACAA CAAAGATGAT GCTGAAAAGG TCGCAGTTAC TCGAATAGGA 
GGTCGGAAAC GGATTACAAA GGAACAAAAG AAGCTGTGTT ATTCAGAACC AGAGAAATTA 
GATCACCTTG GGATTCAAAA ATCTAACTTT GCATGGAAGG AGGAACCTGC TATCGCAAAT 
AAAAGGGAGA TGAGTGATTC TGATGTTGCT GCAAATAAGA TAAAGTATCT GGAGAGCAAA 
GAACGAACTG CCTCATCTTC AAACCTTTCT AAGACAGAAC TCAAACAGAT CCAGCATAAT 
GCGCTTGTTG AATATATGGA AAGGAAAACA AACCAAAGGC CCAGCAGCAA TCCACAATTC 
CAGATGGAAA GGACATCGGG ATTGCCAAAT TATAGTGAGT GGAATATCTA CTCATCTGAA 
ACCAGTTCTG GTGATGGTTC ACAAAAGTAC CTACGGAGGA GATCAGCTGG AGCTTCATCT 
TCCTATGATG CCACAGTAAC ATGGAATGAT AGATTTGGAA AGACTTCACC TCTTGGGCGG 
CGCTCTGCTG TGGAACCAAC TGCTGGTGTG CAACGCAGGA GTTTTTTTGA TCAACGCACT 
TTAGATGGCA GTCAAGAACG TCTTGAAGGT TCTTCCCCAT CTCTCAGTCA AAAAACATCA 
AAGTCAAGTC ATCGTGAACA GGTCTCGTAT GTAAATATGG AATTTCTTCC CTCAGCACTT 
TCCAAGAATC ACATTTATAA TGACCGGCTG ACTGTACCCG GGGACAACAC CAGCGCCGAC 
ACTGAGAGAA TTCGTGCTTC CAAAAGCAGA GGGAAGTCTA TGGAAGAAAT AGGGACTGCA 
GACATTGTAA CGCTTGCAGA GCTGAGCCAT AGCTCAGATC AACTTTACCA CATTAAAGGG 
CCTGTGATAT CGCCAAGGCT GGAAAACACA AGGACTGCAG CTGTGTCCCA TCAAGACAAC 
GTCTTGGCTA GCACTCAGAA CGAAACAGGA AATCTGCCCA GACAAACACA TCAAGAAAGT 
GTTCTGGGGC CCCGCAGATC AGATTTAGCA AATTTGGGAC AAGAGGCACA TTCCTGGCCC 
TTGAGAGCTA GTGCTGCTTC CTCAGGCATA GATAATTCCT GCTCTTCTCC TCCTTCGGCA 
AAGGTTCACC CCTGGGCTCC TGAGCCATTG CATTGCTTGG ACACAGAAGA TGAGGTCTTT 
ACACAAGCCA GCACTGCCAG AAATGAAGAT CCCAGCTCCA CTGCCCTTAG CCATCTGCAC 
GATATTGGAA ACCCTGTGTG TGAAGAGGAA GATACTGCAT TATGCTTTTC TTTCTTACCT 
GAGCAGGGTC ATCTAGAACG GCTGATTGTC AATGAGGCTG CACCAAGCTC CAAGACTGAT 
GAAGGTGTTT TGGACACTGC TGCTGAGAAT GCGACGACAA CGCAACCTCC TGAGACTTCA 
GAAGACAACA AGCCACATGG CTTTAGTGCT GAGAATCAAG AAGTTGAAGA AGGCAGAGAA 
CCTATGCTAG CAGAGTTTGT AGACAGCTCA CAGCAGCTAG AGTTGAGGTC CCTTCCTTCT 
AGCCAGGTTA ACGTAATGCA GACTACAGAG CCATATGTCG GTGATGACAA GAGCAGTGGA 
AATGAAGATT CCATGGAGCA AAAATCACAG GACATTGAGC AGAAAAGTAA GGAACCAGAA 
GAGGAAGATT TACCCAAGGT GAAGTTAAAA TCTCCAGAAG ATGAAAGACG AGAGGAACTG 
GTTAAAGACA TAGTTGCCAA GGATAAATCT CTGATGAATT GTCTCCAGCC GATTTCTGTC 
AGAGAATCTG CTATGGATCT CATGAAAAGC CTCTTCCCTA TGGACTTCAC TGAAGCGGAA 
AAATCTAGAA CTAGAGGACT TCTTAGAAAA GACAACAGTG AAACATTAAG GAAAAACAAT 
TCAGACCTTG AAAGTTCTTC TAAACTTCCT TCAAAAATAA CCGTGATGCT TCAGAAGAGT 
TCTGCGCGTA GGCCAGATGG GGAAAGCCTA GATGACATCA CTTCAAAGAA GATGGAACTT 
CTTTCTAACA TCGGTTCCAA GCTAGAGGAC CTTTGTGAGC AGAAAGAAAT CCTTTTGGCT 
GATAATAGTA AGAACACGAC GCACGGCAAT AATATTCAGG CGCTAGTGAA AGAGTTGTGT 
AAGCCCAATG AGTTTGAGCG CTACATGATG TTCATTGGGG ATCTGGAGAA AGTGGTGAGC 
CTGCTATTTT CCCTGTCGAC AAGGCTGACT CGGGTTGAAA ATGCTTTAAG CAAGGTTGAT 
GAAAATACAG ATGCAGAAGA GATGCAATCC CTAAAGGAGC GCCACAATCT CCTGTCCAGT 
CAAAGAGAAG ATGCCAAAGA CTTAAAGGCA AACTTAGACC GCAGAGAACA AGTTGTCACG 
GGAATCCTTG TAAAATACCT GAATGAGGAG CAGCTGCAGG ACTACAAGCA CTTTGTCAGA 
CTGAAGACCT CCCTCCTAAT TGAACAAAAG AACCTGGAGG AGAAGATAAA ACTGTACGAG 
GAGCAGTTAG AGAGCATCCA CGATAGCATC CCACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shroom1 ( XM_002935260.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935306.2 Xenopus tropicalis shroom family member 1 (shroom1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa07579
Clone ID Related Accession (Same CDS sequence)	XM_004912831.3 , XM_004912832.3 , XM_002935260.4
Accession Version	XM_002935260.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein Shroom1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002935260.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCAT TTGGTAATGC AATTGAGAGA TGGAACATAA AAAGTACAGG AGTCGTTGCA 
GGTCTGGGAC ATTCTGAAAG AATATCCCCC ATAAGATCTA TGACCACATT GGTGGATTCG 
GCCTATAGCT CATTTTCTGG CAGTTCGTAT GTTCCAGAAT ACCAAAACTC ATTTCAGCAT 
GACAGCTGTC ACTTTAATGA TGAACAGCTT TCTTACATGG ACTCTGAATA TGTAAGAGCA 
ATCTATAATC CTAGTGTAGT AGACATGGAT GGTGTATATC ATGATATATT GTCAGGACAT 
AGATGCTCGA ATGTAGCACT ATCTGGAAGA ACTAGCAGTG CCAGCTGTGA CCACCCATCA 
CAAGTGCATC AAACTTCACC AGCAAAACTG GATAACTATC TTACTAACCT TGGTTCTGAC 
CAGTTCAGTG GGACTTATGG GGAATCCATA AATATGAAGC ATAAGCAGAA TCACTCTAAT 
AGTATATATA TGCCTAACCA CAAAGCATAT GGCCTCCAAC GTAACAGTCC TACAGGAGTA 
AGCAGTTTGC AGGAAAAAGA AAACCAAATG TATAATCAAT CAAATTATAT GGAAATAAAA 
GATTATTTTA GTAGATCCTT AGATGTTCTT CAAACAGAAG GAGAGCTCAA GACACAAACC 
TCATGTACTC AGAATCTGCC GGATGAGGTC AGCTGCAAGT TCAGAAACAC ACATTCTACA 
AATAGAACGA GCAAAGACCT TTCCAAGGGA AACGATCTAC AAAAAAGCAA TGAAGAAAAC 
ACAGAGAGAG ACAGCCCATA TCTGACAAAA GAGGGTCCAG TTTCTCAAGG TCAATATGCA 
TCAGATGTAA GGACAAGTTT TAAAAACATA AGACGCTCCC TTAAAAAGTC TGTGTCTGAA 
AAAATTGGTG CTCATGACAA TAAAGGGGGT TGCTGGATTA TGAAGCCAGG TCAAGACCCT 
CATTGTTATA ATTCTGAGGG AACAGTTAAG GACGCAGAGT ATGATGACGT CGAGCAGTGG 
GATATCAGGA AATCAAGACT TAATACAAGG GCAAGCCAGA CTTTATATTG TGAGTCTAAT 
AAAGATGCCT CTTGCCCTCC CTTGAATGCT TTGAATTCTA AGAATGAAGT TCTCGAAGCT 
GATAATACAC TCTGTTTTCA AAAGGATGCA ACTGTAAAGT CTATTCCCCT ATTATCTCAG 
CATATTCAAC AGGAAAAATC TAAAAGCCAT CCCCTGTCTG ATTTAAACTG TGAGAAGATT 
ACCAAGGCAA CAACACCTAT GCTGTATCAT CTTGCTGGAG GAAGGCATAG TGCCTTTATA 
GCCCCTGTAC ACAATACAAA TCCAGCTAAA CAGGAAAAAC TCAAGCTTGA GTCAAAGACA 
TTTGAAAGAA CGAATAACTT CTCTATACTA CAACAAACCG AGCCAAGACC AGATAACCAT 
AAATTCCCCA AGAATAAAAG CCTAACACAA CTTGCTGATC TCCATGACTC TGCAGAGGGT 
GGGAATTCTG GGAATTTAAA TTCTTCAGCA GAAGAGTGCC TAATGAATGA CTACATTGAG 
AAACTGAAGG TTGCCCAGAA GAAAGTATTG AGGGAAACCT CTTTTAAAAG AAAGGACTTG 
CAGATGAGTT TGCCTTGCAG ATTCAAGTTG AATCCACCCA AAAGGCCTAC CATTGATCAT 
TTTCGATCCT ATTCTTCATC CAGTGCAAAT GAGGAGACGG CATTTCTCCA AACAAAAAAC 
TCTGTTGACA GCACCTACAA CAAAGATGAT GCTGAAAAGG TCGCAGTTAC TCGAATAGGA 
GGTCGGAAAC GGATTACAAA GGAACAAAAG AAGCTGTGTT ATTCAGAACC AGAGAAATTA 
GATCACCTTG GGATTCAAAA ATCTAACTTT GCATGGAAGG AGGAACCTGC TATCGCAAAT 
AAAAGGGAGA TGAGTGATTC TGATGTTGCT GCAAATAAGA TAAAGTATCT GGAGAGCAAA 
GAACGAACTG CCTCATCTTC AAACCTTTCT AAGACAGAAC TCAAACAGAT CCAGCATAAT 
GCGCTTGTTG AATATATGGA AAGGAAAACA AACCAAAGGC CCAGCAGCAA TCCACAATTC 
CAGATGGAAA GGACATCGGG ATTGCCAAAT TATAGTGAGT GGAATATCTA CTCATCTGAA 
ACCAGTTCTG GTGATGGTTC ACAAAAGTAC CTACGGAGGA GATCAGCTGG AGCTTCATCT 
TCCTATGATG CCACAGTAAC ATGGAATGAT AGATTTGGAA AGACTTCACC TCTTGGGCGG 
CGCTCTGCTG TGGAACCAAC TGCTGGTGTG CAACGCAGGA GTTTTTTTGA TCAACGCACT 
TTAGATGGCA GTCAAGAACG TCTTGAAGGT TCTTCCCCAT CTCTCAGTCA AAAAACATCA 
AAGTCAAGTC ATCGTGAACA GGTCTCGTAT GTAAATATGG AATTTCTTCC CTCAGCACTT 
TCCAAGAATC ACATTTATAA TGACCGGCTG ACTGTACCCG GGGACAACAC CAGCGCCGAC 
ACTGAGAGAA TTCGTGCTTC CAAAAGCAGA GGGAAGTCTA TGGAAGAAAT AGGGACTGCA 
GACATTGTAA CGCTTGCAGA GCTGAGCCAT AGCTCAGATC AACTTTACCA CATTAAAGGG 
CCTGTGATAT CGCCAAGGCT GGAAAACACA AGGACTGCAG CTGTGTCCCA TCAAGACAAC 
GTCTTGGCTA GCACTCAGAA CGAAACAGGA AATCTGCCCA GACAAACACA TCAAGAAAGT 
GTTCTGGGGC CCCGCAGATC AGATTTAGCA AATTTGGGAC AAGAGGCACA TTCCTGGCCC 
TTGAGAGCTA GTGCTGCTTC CTCAGGCATA GATAATTCCT GCTCTTCTCC TCCTTCGGCA 
AAGGTTCACC CCTGGGCTCC TGAGCCATTG CATTGCTTGG ACACAGAAGA TGAGGTCTTT 
ACACAAGCCA GCACTGCCAG AAATGAAGAT CCCAGCTCCA CTGCCCTTAG CCATCTGCAC 
GATATTGGAA ACCCTGTGTG TGAAGAGGAA GATACTGCAT TATGCTTTTC TTTCTTACCT 
GAGCAGGGTC ATCTAGAACG GCTGATTGTC AATGAGGCTG CACCAAGCTC CAAGACTGAT 
GAAGGTGTTT TGGACACTGC TGCTGAGAAT GCGACGACAA CGCAACCTCC TGAGACTTCA 
GAAGACAACA AGCCACATGG CTTTAGTGCT GAGAATCAAG AAGTTGAAGA AGGCAGAGAA 
CCTATGCTAG CAGAGTTTGT AGACAGCTCA CAGCAGCTAG AGTTGAGGTC CCTTCCTTCT 
AGCCAGGTTA ACGTAATGCA GACTACAGAG CCATATGTCG GTGATGACAA GAGCAGTGGA 
AATGAAGATT CCATGGAGCA AAAATCACAG GACATTGAGC AGAAAAGTAA GGAACCAGAA 
GAGGAAGATT TACCCAAGGT GAAGTTAAAA TCTCCAGAAG ATGAAAGACG AGAGGAACTG 
GTTAAAGACA TAGTTGCCAA GGATAAATCT CTGATGAATT GTCTCCAGCC GATTTCTGTC 
AGAGAATCTG CTATGGATCT CATGAAAAGC CTCTTCCCTA TGGACTTCAC TGAAGCGGAA 
AAATCTAGAA CTAGAGGACT TCTTAGAAAA GACAACAGTG AAACATTAAG GAAAAACAAT 
TCAGACCTTG AAAGTTCTTC TAAACTTCCT TCAAAAATAA CCGTGATGCT TCAGAAGAGT 
TCTGCGCGTA GGCCAGATGG GGAAAGCCTA GATGACATCA CTTCAAAGAA GATGGAACTT 
CTTTCTAACA TCGGTTCCAA GCTAGAGGAC CTTTGTGAGC AGAAAGAAAT CCTTTTGGCT 
GATAATAGTA AGAACACGAC GCACGGCAAT AATATTCAGG CGCTAGTGAA AGAGTTGTGT 
AAGCCCAATG AGTTTGAGCG CTACATGATG TTCATTGGGG ATCTGGAGAA AGTGGTGAGC 
CTGCTATTTT CCCTGTCGAC AAGGCTGACT CGGGTTGAAA ATGCTTTAAG CAAGGTTGAT 
GAAAATACAG ATGCAGAAGA GATGCAATCC CTAAAGGAGC GCCACAATCT CCTGTCCAGT 
CAAAGAGAAG ATGCCAAAGA CTTAAAGGCA AACTTAGACC GCAGAGAACA AGTTGTCACG 
GGAATCCTTG TAAAATACCT GAATGAGGAG CAGCTGCAGG ACTACAAGCA CTTTGTCAGA 
CTGAAGACCT CCCTCCTAAT TGAACAAAAG AACCTGGAGG AGAAGATAAA ACTGTACGAG 
GAGCAGTTAG AGAGCATCCA CGATAGCATC CCACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935306.2
CDS	331..4629
Translation	MSSFGNAIERWNIKSTGVVAGLGHSERISPIRSMTTLVDSAYSSFSGSSYVPEYQNSFQHDSCHFNDEQLSYMDSEYVRAIYNPSVVDMDGVYHDILSGHRCSNVALSGRTSSASCDHPSQVHQTSPAKLDNYLTNLGSDQFSGTYGESINMKHKQNHSNSIYMPNHKAYGLQRNSPTGVSSLQEKENQMYNQSNYMEIKDYFSRSLDVLQTEGELKTQTSCTQNLPDEVSCKFRNTHSTNRTSKDLSKGNDLQKSNEENTERDSPYLTKEGPVSQGQYASDVRTSFKNIRRSLKKSVSEKIGAHDNKGGCWIMKPGQDPHCYNSEGTVKDAEYDDVEQWDIRKSRLNTRASQTLYCESNKDASCPPLNALNSKNEVLEADNTLCFQKDATVKSIPLLSQHIQQEKSKSHPLSDLNCEKITKATTPMLYHLAGGRHSAFIAPVHNTNPAKQEKLKLESKTFERTNNFSILQQTEPRPDNHKFPKNKSLTQLADLHDSAEGGNSGNLNSSAEECLMNDYIEKLKVAQKKVLRETSFKRKDLQMSLPCRFKLNPPKRPTIDHFRSYSSSSANEETAFLQTKNSVDSTYNKDDAEKVAVTRIGGRKRITKEQKKLCYSEPEKLDHLGIQKSNFAWKEEPAIANKREMSDSDVAANKIKYLESKERTASSSNLSKTELKQIQHNALVEYMERKTNQRPSSNPQFQMERTSGLPNYSEWNIYSSETSSGDGSQKYLRRRSAGASSSYDATVTWNDRFGKTSPLGRRSAVEPTAGVQRRSFFDQRTLDGSQERLEGSSPSLSQKTSKSSHREQVSYVNMEFLPSALSKNHIYNDRLTVPGDNTSADTERIRASKSRGKSMEEIGTADIVTLAELSHSSDQLYHIKGPVISPRLENTRTAAVSHQDNVLASTQNETGNLPRQTHQESVLGPRRSDLANLGQEAHSWPLRASAASSGIDNSCSSPPSAKVHPWAPEPLHCLDTEDEVFTQASTARNEDPSSTALSHLHDIGNPVCEEEDTALCFSFLPEQGHLERLIVNEAAPSSKTDEGVLDTAAENATTTQPPETSEDNKPHGFSAENQEVEEGREPMLAEFVDSSQQLELRSLPSSQVNVMQTTEPYVGDDKSSGNEDSMEQKSQDIEQKSKEPEEEDLPKVKLKSPEDERREELVKDIVAKDKSLMNCLQPISVRESAMDLMKSLFPMDFTEAEKSRTRGLLRKDNSETLRKNNSDLESSSKLPSKITVMLQKSSARRPDGESLDDITSKKMELLSNIGSKLEDLCEQKEILLADNSKNTTHGNNIQALVKELCKPNEFERYMMFIGDLEKVVSLLFSLSTRLTRVENALSKVDENTDAEEMQSLKERHNLLSSQREDAKDLKANLDRREQVVTGILVKYLNEEQLQDYKHFVRLKTSLLIEQKNLEEKIKLYEEQLESIHDSIPP

Target ORF information:

RefSeq Version	XM_002935260.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis shroom family member 1 (shroom1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935260.4

ORF Insert Sequence:

ATGTCATCAT TTGGTAATGC AATTGAGAGA TGGAACATAA AAAGTACAGG AGTCGTTGCA 
GGTCTGGGAC ATTCTGAAAG AATATCCCCC ATAAGATCTA TGACCACATT GGTGGATTCG 
GCCTATAGCT CATTTTCTGG CAGTTCGTAT GTTCCAGAAT ACCAAAACTC ATTTCAGCAT 
GACAGCTGTC ACTTTAATGA TGAACAGCTT TCTTACATGG ACTCTGAATA TGTAAGAGCA 
ATCTATAATC CTAGTGTAGT AGACATGGAT GGTGTATATC ATGATATATT GTCAGGACAT 
AGATGCTCGA ATGTAGCACT ATCTGGAAGA ACTAGCAGTG CCAGCTGTGA CCACCCATCA 
CAAGTGCATC AAACTTCACC AGCAAAACTG GATAACTATC TTACTAACCT TGGTTCTGAC 
CAGTTCAGTG GGACTTATGG GGAATCCATA AATATGAAGC ATAAGCAGAA TCACTCTAAT 
AGTATATATA TGCCTAACCA CAAAGCATAT GGCCTCCAAC GTAACAGTCC TACAGGAGTA 
AGCAGTTTGC AGGAAAAAGA AAACCAAATG TATAATCAAT CAAATTATAT GGAAATAAAA 
GATTATTTTA GTAGATCCTT AGATGTTCTT CAAACAGAAG GAGAGCTCAA GACACAAACC 
TCATGTACTC AGAATCTGCC GGATGAGGTC AGCTGCAAGT TCAGAAACAC ACATTCTACA 
AATAGAACGA GCAAAGACCT TTCCAAGGGA AACGATCTAC AAAAAAGCAA TGAAGAAAAC 
ACAGAGAGAG ACAGCCCATA TCTGACAAAA GAGGGTCCAG TTTCTCAAGG TCAATATGCA 
TCAGATGTAA GGACAAGTTT TAAAAACATA AGACGCTCCC TTAAAAAGTC TGTGTCTGAA 
AAAATTGGTG CTCATGACAA TAAAGGGGGT TGCTGGATTA TGAAGCCAGG TCAAGACCCT 
CATTGTTATA ATTCTGAGGG AACAGTTAAG GACGCAGAGT ATGATGACGT CGAGCAGTGG 
GATATCAGGA AATCAAGACT TAATACAAGG GCAAGCCAGA CTTTATATTG TGAGTCTAAT 
AAAGATGCCT CTTGCCCTCC CTTGAATGCT TTGAATTCTA AGAATGAAGT TCTCGAAGCT 
GATAATACAC TCTGTTTTCA AAAGGATGCA ACTGTAAAGT CTATTCCCCT ATTATCTCAG 
CATATTCAAC AGGAAAAATC TAAAAGCCAT CCCCTGTCTG ATTTAAACTG TGAGAAGATT 
ACCAAGGCAA CAACACCTAT GCTGTATCAT CTTGCTGGAG GAAGGCATAG TGCCTTTATA 
GCCCCTGTAC ACAATACAAA TCCAGCTAAA CAGGAAAAAC TCAAGCTTGA GTCAAAGACA 
TTTGAAAGAA CGAATAACTT CTCTATACTA CAACAAACCG AGCCAAGACC AGATAACCAT 
AAATTCCCCA AGAATAAAAG CCTAACACAA CTTGCTGATC TCCATGACTC TGCAGAGGGT 
GGGAATTCTG GGAATTTAAA TTCTTCAGCA GAAGAGTGCC TAATGAATGA CTACATTGAG 
AAACTGAAGG TTGCCCAGAA GAAAGTATTG AGGGAAACCT CTTTTAAAAG AAAGGACTTG 
CAGATGAGTT TGCCTTGCAG ATTCAAGTTG AATCCACCCA AAAGGCCTAC CATTGATCAT 
TTTCGATCCT ATTCTTCATC CAGTGCAAAT GAGGAGACGG CATTTCTCCA AACAAAAAAC 
TCTGTTGACA GCACCTACAA CAAAGATGAT GCTGAAAAGG TCGCAGTTAC TCGAATAGGA 
GGTCGGAAAC GGATTACAAA GGAACAAAAG AAGCTGTGTT ATTCAGAACC AGAGAAATTA 
GATCACCTTG GGATTCAAAA ATCTAACTTT GCATGGAAGG AGGAACCTGC TATCGCAAAT 
AAAAGGGAGA TGAGTGATTC TGATGTTGCT GCAAATAAGA TAAAGTATCT GGAGAGCAAA 
GAACGAACTG CCTCATCTTC AAACCTTTCT AAGACAGAAC TCAAACAGAT CCAGCATAAT 
GCGCTTGTTG AATATATGGA AAGGAAAACA AACCAAAGGC CCAGCAGCAA TCCACAATTC 
CAGATGGAAA GGACATCGGG ATTGCCAAAT TATAGTGAGT GGAATATCTA CTCATCTGAA 
ACCAGTTCTG GTGATGGTTC ACAAAAGTAC CTACGGAGGA GATCAGCTGG AGCTTCATCT 
TCCTATGATG CCACAGTAAC ATGGAATGAT AGATTTGGAA AGACTTCACC TCTTGGGCGG 
CGCTCTGCTG TGGAACCAAC TGCTGGTGTG CAACGCAGGA GTTTTTTTGA TCAACGCACT 
TTAGATGGCA GTCAAGAACG TCTTGAAGGT TCTTCCCCAT CTCTCAGTCA AAAAACATCA 
AAGTCAAGTC ATCGTGAACA GGTCTCGTAT GTAAATATGG AATTTCTTCC CTCAGCACTT 
TCCAAGAATC ACATTTATAA TGACCGGCTG ACTGTACCCG GGGACAACAC CAGCGCCGAC 
ACTGAGAGAA TTCGTGCTTC CAAAAGCAGA GGGAAGTCTA TGGAAGAAAT AGGGACTGCA 
GACATTGTAA CGCTTGCAGA GCTGAGCCAT AGCTCAGATC AACTTTACCA CATTAAAGGG 
CCTGTGATAT CGCCAAGGCT GGAAAACACA AGGACTGCAG CTGTGTCCCA TCAAGACAAC 
GTCTTGGCTA GCACTCAGAA CGAAACAGGA AATCTGCCCA GACAAACACA TCAAGAAAGT 
GTTCTGGGGC CCCGCAGATC AGATTTAGCA AATTTGGGAC AAGAGGCACA TTCCTGGCCC 
TTGAGAGCTA GTGCTGCTTC CTCAGGCATA GATAATTCCT GCTCTTCTCC TCCTTCGGCA 
AAGGTTCACC CCTGGGCTCC TGAGCCATTG CATTGCTTGG ACACAGAAGA TGAGGTCTTT 
ACACAAGCCA GCACTGCCAG AAATGAAGAT CCCAGCTCCA CTGCCCTTAG CCATCTGCAC 
GATATTGGAA ACCCTGTGTG TGAAGAGGAA GATACTGCAT TATGCTTTTC TTTCTTACCT 
GAGCAGGGTC ATCTAGAACG GCTGATTGTC AATGAGGCTG CACCAAGCTC CAAGACTGAT 
GAAGGTGTTT TGGACACTGC TGCTGAGAAT GCGACGACAA CGCAACCTCC TGAGACTTCA 
GAAGACAACA AGCCACATGG CTTTAGTGCT GAGAATCAAG AAGTTGAAGA AGGCAGAGAA 
CCTATGCTAG CAGAGTTTGT AGACAGCTCA CAGCAGCTAG AGTTGAGGTC CCTTCCTTCT 
AGCCAGGTTA ACGTAATGCA GACTACAGAG CCATATGTCG GTGATGACAA GAGCAGTGGA 
AATGAAGATT CCATGGAGCA AAAATCACAG GACATTGAGC AGAAAAGTAA GGAACCAGAA 
GAGGAAGATT TACCCAAGGT GAAGTTAAAA TCTCCAGAAG ATGAAAGACG AGAGGAACTG 
GTTAAAGACA TAGTTGCCAA GGATAAATCT CTGATGAATT GTCTCCAGCC GATTTCTGTC 
AGAGAATCTG CTATGGATCT CATGAAAAGC CTCTTCCCTA TGGACTTCAC TGAAGCGGAA 
AAATCTAGAA CTAGAGGACT TCTTAGAAAA GACAACAGTG AAACATTAAG GAAAAACAAT 
TCAGACCTTG AAAGTTCTTC TAAACTTCCT TCAAAAATAA CCGTGATGCT TCAGAAGAGT 
TCTGCGCGTA GGCCAGATGG GGAAAGCCTA GATGACATCA CTTCAAAGAA GATGGAACTT 
CTTTCTAACA TCGGTTCCAA GCTAGAGGAC CTTTGTGAGC AGAAAGAAAT CCTTTTGGCT 
GATAATAGTA AGAACACGAC GCACGGCAAT AATATTCAGG CGCTAGTGAA AGAGTTGTGT 
AAGCCCAATG AGTTTGAGCG CTACATGATG TTCATTGGGG ATCTGGAGAA AGTGGTGAGC 
CTGCTATTTT CCCTGTCGAC AAGGCTGACT CGGGTTGAAA ATGCTTTAAG CAAGGTTGAT 
GAAAATACAG ATGCAGAAGA GATGCAATCC CTAAAGGAGC GCCACAATCT CCTGTCCAGT 
CAAAGAGAAG ATGCCAAAGA CTTAAAGGCA AACTTAGACC GCAGAGAACA AGTTGTCACG 
GGAATCCTTG TAAAATACCT GAATGAGGAG CAGCTGCAGG ACTACAAGCA CTTTGTCAGA 
CTGAAGACCT CCCTCCTAAT TGAACAAAAG AACCTGGAGG AGAAGATAAA ACTGTACGAG 
GAGCAGTTAG AGAGCATCCA CGATAGCATC CCACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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shroom1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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