aspg cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following aspg gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the aspg cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa02157 NM_001127027.1
Latest version!
Xenopus tropicalis asparaginase (aspg), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OXa31920 XM_012968032.3
Latest version!
Xenopus tropicalis asparaginase (aspg), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
View Old Accession Versions >>
OXa31920 XM_012968032.2 Xenopus tropicalis asparaginase (aspg), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa02157
    Clone ID Related Accession (Same CDS sequence) NM_001127027.1
    Accession Version NM_001127027.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1851bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Xenopus tropicalis(tropical clawed frog)
    Product 60 kDa lysophospholipase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC161380.1. ##Evidence-Data-START## Transcript exon combination :: BC161380.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028290, SAMD00028291 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGCCCCCAC CACCCCCCAG CCAAGCTCCG GCTCCCACAG CCCGTGTATC TGCATCGCAG 
    ACCCCACGGA GGAGAAAGCT GTCCAGCAGC CAGAGCCTGT CCGGCTCCTT GGAAAGCCTG
    AGCGGTATTG GGAGCGAGGC TCGGGTGCTA GTTATTAACA CGGGGGGCAC AATAGGGATG
    ATGTACCAGA ATGAAGTGCT GGCTCCTCAG GCCAATGCTT TTGCAAAAAC CCTAAAAACC
    CTGCCTATCC TTCATGATGA GGTATATGCC CAGCAAACCA AGCTCTATGA TTTTCATGAC
    TTTCCAGAAA ACACATTGGT CCTCCCTCTC TCTAAACAGA ACAAAAGAAT TGTGTACACT
    GTCTTGGAGT ATTCGCCTCT TCTCGACTCC TGCAATATGA CTACAGATGA CTGGGCCAAA
    ATTGCTAAGG ATATCAAGAA ATACTACGAG CAATATGATG GGTTTGTGGT GCTACATGGT
    ACTGATACTA TGGCCTATAC AGCATCTGCA CTTTCATTTA TGTGTGAAAA TCTGGGGAAA
    ACAATAGTCT TGACTGGATC TCAGGTACCT ATTTATGAGA TGAGAAATGA TGGCCGTGAC
    AATCTGCTTG GAGCTCTGCT TATTGCAGGA CAGTTTGTTA TTCCCGAGGT GTGCCTGTAT
    TTTCATCACA AGCTCTACAG GGGAAACAGG GTCACTAAGG TGGATGCTGG GAGTTTTAAT
    GCTTTCACAT CTCCGAACTT GCCTCCACTT GCAAATGCTG AAGTTGACAT TACAATTAAT
    TGGGAGACCG TATGGCGAGC TAATACCACC AAGAAGTTTC ATGTCCATAC TGATATGAAT
    AGGAATGTGG GCCTACTGCG AATATTTCCT GGGATAACTG CTGCAACTGT GAAGGCCTTT
    CTGCAGCCCC CTATGGAAGG CATTGTGCTT GAAACATATG GTTCTGGCAA TGCCCCAGAT
    TGTCGCTTGG ATTTGCTTGA AGAGCTTAAG AAGGCTACAG AGCGTGGCGT TGCTATTATA
    AATTGTACCC AGTGCTTGCG AGGATCCGTC ACTGAATCAT ATGCAACTGG AAAGGCTCTG
    ACTGTGGTAG GAGTCATTCC TGGATGTGAT TTGACACCAG AAGCAGCTCT CGCAAAACTT
    TCCTATGTTT TAGGGAAAGA CAACCTCAAT CCAGAGGAAA AAAAAAGGAT GCTGTTTGAG
    AATCTCCGGG GAGAGATGAC CGTAAGCGCC ACAGGAGCCA AGATCTCTCT GCGAGACAGC
    AAGTTCATCC AAGTAGTTGC TAAATCTCTT GGCGTGAGCT GCAAAGAGGA GCTAGAAGCT
    GTCAGAGATG CCCTCACACC ATCTTTAGCC TGTGCTGCCG CTGCCACTGG TGATACCTAT
    GCTTTGGAAG CAATAAGAGA CATGGGTGGG AACTTGAGCA GTGAAGATTA TGATGGCCGG
    ACTCCATTGC ATGTGGCATC CTGTGATGGT CATTATGCTG CTGTCCAGTA CTTGCTGAAT
    AACGGAGCCA CTGTTTATGC TAAGGATCGT TTTGGTGACA CACCTCTGAT GAATGCTGTG
    AAATTCAGAC AATTGGAAGT TATCAAGTTG CTCCGACAGA CTGGTGCACA TCTTTCCTGG
    GAGGAGCTTG AACACACTGG CACAGAGTTG TGCAGTTTAG CTTCTCTTGG TGATGTGGAT
    GGCCTTGCAG CCTGGGAATT AGCAGGAGCA GATATGACTC AAGCTGGATA TGATGGACAA
    ACTCCTTACC AAGTGGCAGA AGCTGCAGGC AATGAAGAAG TCTTAGAATT CTTTAGCCTA
    AAAAGGCCAC AGGTCTTTCT AAATGGCATT GCAAATGAAA ATGGAAACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001120499.1
    CDS67..1917
    Translation

    Target ORF information:

    RefSeq Version NM_001127027.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis asparaginase (aspg), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001127027.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGCCCCCAC CACCCCCCAG CCAAGCTCCG GCTCCCACAG CCCGTGTATC TGCATCGCAG 
    ACCCCACGGA GGAGAAAGCT GTCCAGCAGC CAGAGCCTGT CCGGCTCCTT GGAAAGCCTG
    AGCGGTATTG GGAGCGAGGC TCGGGTGCTA GTTATTAACA CGGGGGGCAC AATAGGGATG
    ATGTACCAGA ATGAAGTGCT GGCTCCTCAG GCCAATGCTT TTGCAAAAAC CCTAAAAACC
    CTGCCTATCC TTCATGATGA GGTATATGCC CAGCAAACCA AGCTCTATGA TTTTCATGAC
    TTTCCAGAAA ACACATTGGT CCTCCCTCTC TCTAAACAGA ACAAAAGAAT TGTGTACACT
    GTCTTGGAGT ATTCGCCTCT TCTCGACTCC TGCAATATGA CTACAGATGA CTGGGCCAAA
    ATTGCTAAGG ATATCAAGAA ATACTACGAG CAATATGATG GGTTTGTGGT GCTACATGGT
    ACTGATACTA TGGCCTATAC AGCATCTGCA CTTTCATTTA TGTGTGAAAA TCTGGGGAAA
    ACAATAGTCT TGACTGGATC TCAGGTACCT ATTTATGAGA TGAGAAATGA TGGCCGTGAC
    AATCTGCTTG GAGCTCTGCT TATTGCAGGA CAGTTTGTTA TTCCCGAGGT GTGCCTGTAT
    TTTCATCACA AGCTCTACAG GGGAAACAGG GTCACTAAGG TGGATGCTGG GAGTTTTAAT
    GCTTTCACAT CTCCGAACTT GCCTCCACTT GCAAATGCTG AAGTTGACAT TACAATTAAT
    TGGGAGACCG TATGGCGAGC TAATACCACC AAGAAGTTTC ATGTCCATAC TGATATGAAT
    AGGAATGTGG GCCTACTGCG AATATTTCCT GGGATAACTG CTGCAACTGT GAAGGCCTTT
    CTGCAGCCCC CTATGGAAGG CATTGTGCTT GAAACATATG GTTCTGGCAA TGCCCCAGAT
    TGTCGCTTGG ATTTGCTTGA AGAGCTTAAG AAGGCTACAG AGCGTGGCGT TGCTATTATA
    AATTGTACCC AGTGCTTGCG AGGATCCGTC ACTGAATCAT ATGCAACTGG AAAGGCTCTG
    ACTGTGGTAG GAGTCATTCC TGGATGTGAT TTGACACCAG AAGCAGCTCT CGCAAAACTT
    TCCTATGTTT TAGGGAAAGA CAACCTCAAT CCAGAGGAAA AAAAAAGGAT GCTGTTTGAG
    AATCTCCGGG GAGAGATGAC CGTAAGCGCC ACAGGAGCCA AGATCTCTCT GCGAGACAGC
    AAGTTCATCC AAGTAGTTGC TAAATCTCTT GGCGTGAGCT GCAAAGAGGA GCTAGAAGCT
    GTCAGAGATG CCCTCACACC ATCTTTAGCC TGTGCTGCCG CTGCCACTGG TGATACCTAT
    GCTTTGGAAG CAATAAGAGA CATGGGTGGG AACTTGAGCA GTGAAGATTA TGATGGCCGG
    ACTCCATTGC ATGTGGCATC CTGTGATGGT CATTATGCTG CTGTCCAGTA CTTGCTGAAT
    AACGGAGCCA CTGTTTATGC TAAGGATCGT TTTGGTGACA CACCTCTGAT GAATGCTGTG
    AAATTCAGAC AATTGGAAGT TATCAAGTTG CTCCGACAGA CTGGTGCACA TCTTTCCTGG
    GAGGAGCTTG AACACACTGG CACAGAGTTG TGCAGTTTAG CTTCTCTTGG TGATGTGGAT
    GGCCTTGCAG CCTGGGAATT AGCAGGAGCA GATATGACTC AAGCTGGATA TGATGGACAA
    ACTCCTTACC AAGTGGCAGA AGCTGCAGGC AATGAAGAAG TCTTAGAATT CTTTAGCCTA
    AAAAGGCCAC AGGTCTTTCT AAATGGCATT GCAAATGAAA ATGGAAACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31920
    Clone ID Related Accession (Same CDS sequence) XM_012968032.2 , XM_012968032.3
    Accession Version XM_012968032.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1947bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product 60 kDa lysophospholipase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968032.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGCGGAGG AAGAGAAGAG TCTGTTGTCC CGGTTATCCA AGGCGCTATC TCGTACCCGA 
    CTGGAGCAGC TGGAGCAAGA AGCCGCTATG CCCCCACCAC CCCCCAGCCA AGCTCCGGCT
    CCCACAGCCC GTGTATCTGC ATCGCAGACC CCACGGAGGA GAAAGCTGTC CAGCAGCCAG
    AGCCTGTCCG GCTCCTTGGA AAGCCTGAGC GGTATTGGGA GCGAGGCTCG GGTGCTAGTT
    ATTAACACGG GGGGCACAAT AGGGATGATG TACCAGAATG AAGTGCTGGC TCCTCAGGCC
    AATGCTTTTG CAAAAACCCT AAAAACCCTG CCTATCCTTC ATGATGAGGT ATATGCCCAG
    CAAACCAAGC TCTATGATTT TCATGACTTT CCAGAAAACA CATTGGTCCT CCCTCTCTCT
    AAACAGAACA AAAGAATTGT GTACACTGTC TTGGAGTATT CGCCTCTTCT CGACTCCTGC
    AATATGACTA CAGATGACTG GGCCAAAATT GCTAAGGATA TCAAGAAATA CTACGAGCAA
    TATGATGGGT TTGTGGTGCT ACATGGTACT GATACTATGG CCTATACAGC ATCTGCACTT
    TCATTTATGT GTGAAAATCT GGGGAAAACA ATAGTCTTGA CTGGATCTCA GGTACCTATT
    TATGAGATGA GAAATGATGG CCGTGACAAT CTGCTTGGAG CTCTGCTTAT TGCAGGACAG
    TTTGTTATTC CCGAGGTGTG CCTGTATTTT CATCACAAGC TCTACAGGGG AAACAGGGTC
    ACTAAGGTGG ATGCTGGGAG TTTTAATGCT TTCACATCTC CGAACTTGCC TCCACTTGCA
    AATGCTGAAG TTGACATTAC AATTAATTGG GAGACCGTAT GGCGAGCTAA TACCACCAAG
    AAGTTTCATG TCCATACTGA TATGAATAGG AATGTGGGCC TACTGCGAAT ATTTCCTGGG
    ATAACTGCTG CAACTGTGAA GGCCTTTCTG CAGCCCCCTA TGGAAGGCAT TGTGCTTGAA
    ACATATGGTT CTGGCAATGC CCCAGATTGT CGCTTGGATT TGCTTGAAGA GCTTAAGAAG
    GCTACAGAGC GTGGCGTTGC TATTATAAAT TGTACCCAGT GCTTGCGAGG ATCCGTCACT
    GAATCATATG CAACTGGAAA GGCTCTGACT GTGGTAGGAG TCATTCCTGG ATGTGATTTG
    ACACCAGAAG CAGCTCTCGC AAAACTTTCC TATGTTTTAG GGAAAGACAA CCTCAATCCA
    GAGGAAAAAA AAAGGATGCT GTTTGAGAAT CTCCGGGGAG AGATGACCGT AAGCGCCACA
    GGAGCCAAGA TCTCTCTGCG AGACAGCAAG TTCATCCAAG TAGTTGCTAA ATCTCTTGGC
    GTGAGCTGCA AAGAGGAGCT AGAAGCTGTC AGAGATGCCC TCACACCATC TTTAGCCTGT
    GCTGCCGCTG CCACTGGTGA TACCTATGCT TTGGAAGCAA TAAGAGACAT GGGTGGGAAC
    TTGAGCAGTG AAGATTATGA TGGCCGGACT CCATTGCATG TGGCATCCTG TGATGGTCAT
    TATGCTGCTG TCCAGTACTT GCTGAATAAC GGAGCCACTG TTTATGCTAA GGATCGTTTT
    GGTGACACAC CTCTGATGAA TGCTGTGAAA TTCAGACAAT TGGAAGTTAT CAAGTTGCTC
    CGACAGACTG GTGCACATCT TTCCTGGGAG GAGCTTGAAC ACACTGGCAC AGAGTTGTGC
    AGTTTAGCTT CTCTTGGTGA TGTGGATGGC CTTGCAGCCT GGGAATTAGC AGGAGCAGAT
    ATGACTCAAG CTGGATATGA TGGACAAACT CCTTACCAAG TGGCAGAAGC TGCAGGCAAT
    GAAGAAGTCT TAGAATTCTT TAGCCTAAAA AGGCCACAGA ATCTCTCACC AGTGAGGTTT
    TTCCTCTACT CTGGAACTGA AACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012823486.1
    CDS1457..3403
    Translation

    Target ORF information:

    RefSeq Version XM_012968032.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis asparaginase (aspg), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012968032.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGCGGAGG AAGAGAAGAG TCTGTTGTCC CGGTTATCCA AGGCGCTATC TCGTACCCGA 
    CTGGAGCAGC TGGAGCAAGA AGCCGCTATG CCCCCACCAC CCCCCAGCCA AGCTCCGGCT
    CCCACAGCCC GTGTATCTGC ATCGCAGACC CCACGGAGGA GAAAGCTGTC CAGCAGCCAG
    AGCCTGTCCG GCTCCTTGGA AAGCCTGAGC GGTATTGGGA GCGAGGCTCG GGTGCTAGTT
    ATTAACACGG GGGGCACAAT AGGGATGATG TACCAGAATG AAGTGCTGGC TCCTCAGGCC
    AATGCTTTTG CAAAAACCCT AAAAACCCTG CCTATCCTTC ATGATGAGGT ATATGCCCAG
    CAAACCAAGC TCTATGATTT TCATGACTTT CCAGAAAACA CATTGGTCCT CCCTCTCTCT
    AAACAGAACA AAAGAATTGT GTACACTGTC TTGGAGTATT CGCCTCTTCT CGACTCCTGC
    AATATGACTA CAGATGACTG GGCCAAAATT GCTAAGGATA TCAAGAAATA CTACGAGCAA
    TATGATGGGT TTGTGGTGCT ACATGGTACT GATACTATGG CCTATACAGC ATCTGCACTT
    TCATTTATGT GTGAAAATCT GGGGAAAACA ATAGTCTTGA CTGGATCTCA GGTACCTATT
    TATGAGATGA GAAATGATGG CCGTGACAAT CTGCTTGGAG CTCTGCTTAT TGCAGGACAG
    TTTGTTATTC CCGAGGTGTG CCTGTATTTT CATCACAAGC TCTACAGGGG AAACAGGGTC
    ACTAAGGTGG ATGCTGGGAG TTTTAATGCT TTCACATCTC CGAACTTGCC TCCACTTGCA
    AATGCTGAAG TTGACATTAC AATTAATTGG GAGACCGTAT GGCGAGCTAA TACCACCAAG
    AAGTTTCATG TCCATACTGA TATGAATAGG AATGTGGGCC TACTGCGAAT ATTTCCTGGG
    ATAACTGCTG CAACTGTGAA GGCCTTTCTG CAGCCCCCTA TGGAAGGCAT TGTGCTTGAA
    ACATATGGTT CTGGCAATGC CCCAGATTGT CGCTTGGATT TGCTTGAAGA GCTTAAGAAG
    GCTACAGAGC GTGGCGTTGC TATTATAAAT TGTACCCAGT GCTTGCGAGG ATCCGTCACT
    GAATCATATG CAACTGGAAA GGCTCTGACT GTGGTAGGAG TCATTCCTGG ATGTGATTTG
    ACACCAGAAG CAGCTCTCGC AAAACTTTCC TATGTTTTAG GGAAAGACAA CCTCAATCCA
    GAGGAAAAAA AAAGGATGCT GTTTGAGAAT CTCCGGGGAG AGATGACCGT AAGCGCCACA
    GGAGCCAAGA TCTCTCTGCG AGACAGCAAG TTCATCCAAG TAGTTGCTAA ATCTCTTGGC
    GTGAGCTGCA AAGAGGAGCT AGAAGCTGTC AGAGATGCCC TCACACCATC TTTAGCCTGT
    GCTGCCGCTG CCACTGGTGA TACCTATGCT TTGGAAGCAA TAAGAGACAT GGGTGGGAAC
    TTGAGCAGTG AAGATTATGA TGGCCGGACT CCATTGCATG TGGCATCCTG TGATGGTCAT
    TATGCTGCTG TCCAGTACTT GCTGAATAAC GGAGCCACTG TTTATGCTAA GGATCGTTTT
    GGTGACACAC CTCTGATGAA TGCTGTGAAA TTCAGACAAT TGGAAGTTAT CAAGTTGCTC
    CGACAGACTG GTGCACATCT TTCCTGGGAG GAGCTTGAAC ACACTGGCAC AGAGTTGTGC
    AGTTTAGCTT CTCTTGGTGA TGTGGATGGC CTTGCAGCCT GGGAATTAGC AGGAGCAGAT
    ATGACTCAAG CTGGATATGA TGGACAAACT CCTTACCAAG TGGCAGAAGC TGCAGGCAAT
    GAAGAAGTCT TAGAATTCTT TAGCCTAAAA AGGCCACAGA ATCTCTCACC AGTGAGGTTT
    TTCCTCTACT CTGGAACTGA AACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31920
    Clone ID Related Accession (Same CDS sequence) XM_012968032.2 , XM_012968032.3
    Accession Version XM_012968032.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1947bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product 60 kDa lysophospholipase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012968032.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGCGGAGG AAGAGAAGAG TCTGTTGTCC CGGTTATCCA AGGCGCTATC TCGTACCCGA 
    CTGGAGCAGC TGGAGCAAGA AGCCGCTATG CCCCCACCAC CCCCCAGCCA AGCTCCGGCT
    CCCACAGCCC GTGTATCTGC ATCGCAGACC CCACGGAGGA GAAAGCTGTC CAGCAGCCAG
    AGCCTGTCCG GCTCCTTGGA AAGCCTGAGC GGTATTGGGA GCGAGGCTCG GGTGCTAGTT
    ATTAACACGG GGGGCACAAT AGGGATGATG TACCAGAATG AAGTGCTGGC TCCTCAGGCC
    AATGCTTTTG CAAAAACCCT AAAAACCCTG CCTATCCTTC ATGATGAGGT ATATGCCCAG
    CAAACCAAGC TCTATGATTT TCATGACTTT CCAGAAAACA CATTGGTCCT CCCTCTCTCT
    AAACAGAACA AAAGAATTGT GTACACTGTC TTGGAGTATT CGCCTCTTCT CGACTCCTGC
    AATATGACTA CAGATGACTG GGCCAAAATT GCTAAGGATA TCAAGAAATA CTACGAGCAA
    TATGATGGGT TTGTGGTGCT ACATGGTACT GATACTATGG CCTATACAGC ATCTGCACTT
    TCATTTATGT GTGAAAATCT GGGGAAAACA ATAGTCTTGA CTGGATCTCA GGTACCTATT
    TATGAGATGA GAAATGATGG CCGTGACAAT CTGCTTGGAG CTCTGCTTAT TGCAGGACAG
    TTTGTTATTC CCGAGGTGTG CCTGTATTTT CATCACAAGC TCTACAGGGG AAACAGGGTC
    ACTAAGGTGG ATGCTGGGAG TTTTAATGCT TTCACATCTC CGAACTTGCC TCCACTTGCA
    AATGCTGAAG TTGACATTAC AATTAATTGG GAGACCGTAT GGCGAGCTAA TACCACCAAG
    AAGTTTCATG TCCATACTGA TATGAATAGG AATGTGGGCC TACTGCGAAT ATTTCCTGGG
    ATAACTGCTG CAACTGTGAA GGCCTTTCTG CAGCCCCCTA TGGAAGGCAT TGTGCTTGAA
    ACATATGGTT CTGGCAATGC CCCAGATTGT CGCTTGGATT TGCTTGAAGA GCTTAAGAAG
    GCTACAGAGC GTGGCGTTGC TATTATAAAT TGTACCCAGT GCTTGCGAGG ATCCGTCACT
    GAATCATATG CAACTGGAAA GGCTCTGACT GTGGTAGGAG TCATTCCTGG ATGTGATTTG
    ACACCAGAAG CAGCTCTCGC AAAACTTTCC TATGTTTTAG GGAAAGACAA CCTCAATCCA
    GAGGAAAAAA AAAGGATGCT GTTTGAGAAT CTCCGGGGAG AGATGACCGT AAGCGCCACA
    GGAGCCAAGA TCTCTCTGCG AGACAGCAAG TTCATCCAAG TAGTTGCTAA ATCTCTTGGC
    GTGAGCTGCA AAGAGGAGCT AGAAGCTGTC AGAGATGCCC TCACACCATC TTTAGCCTGT
    GCTGCCGCTG CCACTGGTGA TACCTATGCT TTGGAAGCAA TAAGAGACAT GGGTGGGAAC
    TTGAGCAGTG AAGATTATGA TGGCCGGACT CCATTGCATG TGGCATCCTG TGATGGTCAT
    TATGCTGCTG TCCAGTACTT GCTGAATAAC GGAGCCACTG TTTATGCTAA GGATCGTTTT
    GGTGACACAC CTCTGATGAA TGCTGTGAAA TTCAGACAAT TGGAAGTTAT CAAGTTGCTC
    CGACAGACTG GTGCACATCT TTCCTGGGAG GAGCTTGAAC ACACTGGCAC AGAGTTGTGC
    AGTTTAGCTT CTCTTGGTGA TGTGGATGGC CTTGCAGCCT GGGAATTAGC AGGAGCAGAT
    ATGACTCAAG CTGGATATGA TGGACAAACT CCTTACCAAG TGGCAGAAGC TGCAGGCAAT
    GAAGAAGTCT TAGAATTCTT TAGCCTAAAA AGGCCACAGA ATCTCTCACC AGTGAGGTTT
    TTCCTCTACT CTGGAACTGA AACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012823486.1
    CDS1199..3145
    Translation

    Target ORF information:

    RefSeq Version XM_012968032.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis asparaginase (aspg), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012968032.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGCGGAGG AAGAGAAGAG TCTGTTGTCC CGGTTATCCA AGGCGCTATC TCGTACCCGA 
    CTGGAGCAGC TGGAGCAAGA AGCCGCTATG CCCCCACCAC CCCCCAGCCA AGCTCCGGCT
    CCCACAGCCC GTGTATCTGC ATCGCAGACC CCACGGAGGA GAAAGCTGTC CAGCAGCCAG
    AGCCTGTCCG GCTCCTTGGA AAGCCTGAGC GGTATTGGGA GCGAGGCTCG GGTGCTAGTT
    ATTAACACGG GGGGCACAAT AGGGATGATG TACCAGAATG AAGTGCTGGC TCCTCAGGCC
    AATGCTTTTG CAAAAACCCT AAAAACCCTG CCTATCCTTC ATGATGAGGT ATATGCCCAG
    CAAACCAAGC TCTATGATTT TCATGACTTT CCAGAAAACA CATTGGTCCT CCCTCTCTCT
    AAACAGAACA AAAGAATTGT GTACACTGTC TTGGAGTATT CGCCTCTTCT CGACTCCTGC
    AATATGACTA CAGATGACTG GGCCAAAATT GCTAAGGATA TCAAGAAATA CTACGAGCAA
    TATGATGGGT TTGTGGTGCT ACATGGTACT GATACTATGG CCTATACAGC ATCTGCACTT
    TCATTTATGT GTGAAAATCT GGGGAAAACA ATAGTCTTGA CTGGATCTCA GGTACCTATT
    TATGAGATGA GAAATGATGG CCGTGACAAT CTGCTTGGAG CTCTGCTTAT TGCAGGACAG
    TTTGTTATTC CCGAGGTGTG CCTGTATTTT CATCACAAGC TCTACAGGGG AAACAGGGTC
    ACTAAGGTGG ATGCTGGGAG TTTTAATGCT TTCACATCTC CGAACTTGCC TCCACTTGCA
    AATGCTGAAG TTGACATTAC AATTAATTGG GAGACCGTAT GGCGAGCTAA TACCACCAAG
    AAGTTTCATG TCCATACTGA TATGAATAGG AATGTGGGCC TACTGCGAAT ATTTCCTGGG
    ATAACTGCTG CAACTGTGAA GGCCTTTCTG CAGCCCCCTA TGGAAGGCAT TGTGCTTGAA
    ACATATGGTT CTGGCAATGC CCCAGATTGT CGCTTGGATT TGCTTGAAGA GCTTAAGAAG
    GCTACAGAGC GTGGCGTTGC TATTATAAAT TGTACCCAGT GCTTGCGAGG ATCCGTCACT
    GAATCATATG CAACTGGAAA GGCTCTGACT GTGGTAGGAG TCATTCCTGG ATGTGATTTG
    ACACCAGAAG CAGCTCTCGC AAAACTTTCC TATGTTTTAG GGAAAGACAA CCTCAATCCA
    GAGGAAAAAA AAAGGATGCT GTTTGAGAAT CTCCGGGGAG AGATGACCGT AAGCGCCACA
    GGAGCCAAGA TCTCTCTGCG AGACAGCAAG TTCATCCAAG TAGTTGCTAA ATCTCTTGGC
    GTGAGCTGCA AAGAGGAGCT AGAAGCTGTC AGAGATGCCC TCACACCATC TTTAGCCTGT
    GCTGCCGCTG CCACTGGTGA TACCTATGCT TTGGAAGCAA TAAGAGACAT GGGTGGGAAC
    TTGAGCAGTG AAGATTATGA TGGCCGGACT CCATTGCATG TGGCATCCTG TGATGGTCAT
    TATGCTGCTG TCCAGTACTT GCTGAATAAC GGAGCCACTG TTTATGCTAA GGATCGTTTT
    GGTGACACAC CTCTGATGAA TGCTGTGAAA TTCAGACAAT TGGAAGTTAT CAAGTTGCTC
    CGACAGACTG GTGCACATCT TTCCTGGGAG GAGCTTGAAC ACACTGGCAC AGAGTTGTGC
    AGTTTAGCTT CTCTTGGTGA TGTGGATGGC CTTGCAGCCT GGGAATTAGC AGGAGCAGAT
    ATGACTCAAG CTGGATATGA TGGACAAACT CCTTACCAAG TGGCAGAAGC TGCAGGCAAT
    GAAGAAGTCT TAGAATTCTT TAGCCTAAAA AGGCCACAGA ATCTCTCACC AGTGAGGTTT
    TTCCTCTACT CTGGAACTGA AACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.