trmt2a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	trmt2a
Entrez Gene ID	100145545
Full Name	tRNA methyltransferase 2 homolog A
Synonyms	htf9c
General protein information	Preferred Names tRNA methyltransferase 2 homolog A Names tRNA (uracil-5-)-methyltransferase homolog A TRM2 tRNA methyltransferase 2 homolog A
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031895705.1	XP_031751565.1	tRNA (uracil-5-)-methyltransferase homolog A isoform X3
NM_001126979.2	NP_001120451.2	tRNA (uracil-5-)-methyltransferase homolog A
XM_012956249.2	XP_012811703.1	tRNA (uracil-5-)-methyltransferase homolog A isoform X3
XM_012956249.3	XP_012811703.1	tRNA (uracil-5-)-methyltransferase homolog A isoform X2
XM_012956240.1	XP_012811694.1	tRNA (uracil-5-)-methyltransferase homolog A isoform X1
XM_012956246.2	XP_012811700.1	tRNA (uracil-5-)-methyltransferase homolog A isoform X2
XM_012956240.2	XP_012811694.2	tRNA (uracil-5-)-methyltransferase homolog A isoform X1

Pathways from BioSystems

Homologies

	TRMT2A	XP_002798309.1
Canis lupus familiaris (dog)	TRMT2A	XP_005636628.1
Danio rerio (zebrafish)	trmt2a	NP_956223.1
Caenorhabditis elegans (roundworm)	H24K24.4	NP_503353.2
Homo sapiens (human)	TRMT2A	NP_073564.3
Mus musculus (house mouse)	Trmt2a	NP_001074469.1
Rattus norvegicus (Norway rat)	Trmt2a	NP_001011895.1
Gallus gallus (chicken)	TRMT2A	XP_415080.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012408	XP_320147.4
Xenopus tropicalis (tropical clawed frog)	trmt2a	NP_001120451.2
Pan troglodytes (chimpanzee)	TRMT2A	XP_001167055.1
Bos taurus (cattle)	TRMT2A	XP_002694704.3
Drosophila melanogaster (fruit fly)	CG3808	NP_649083.2

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following trmt2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the trmt2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa21727	NM_001126979.2 Latest version!	Xenopus tropicalis tRNA methyltransferase 2 homolog A (trmt2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OXa31888	XM_012956240.1 Latest version!	Xenopus tropicalis tRNA methyltransferase 2 homolog A (trmt2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OXa31889	XM_012956246.2 Latest version!	Xenopus tropicalis tRNA methyltransferase 2 homolog A (trmt2a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OXa31890	XM_012956249.2 Latest version!	Xenopus tropicalis tRNA methyltransferase 2 homolog A (trmt2a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OXa47583	XM_012956240.2 Latest version!	Xenopus tropicalis tRNA methyltransferase 2 homolog A (trmt2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OXa47584	XM_012956249.3 Latest version!	Xenopus tropicalis tRNA methyltransferase 2 homolog A (trmt2a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OXa47585	XM_031895705.1 Latest version!	Xenopus tropicalis tRNA methyltransferase 2 homolog A (trmt2a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

trmt2a ( NM_001126979.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001120451.2 Xenopus tropicalis tRNA methyltransferase 2 homolog A (trmt2a), mRNA.

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CloneID	OXa21727
Clone ID Related Accession (Same CDS sequence)	NM_001126979.2
Accession Version	NM_001126979.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tRNA (uracil-5-)-methyltransferase homolog A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC161232.1, CR434699.1, CX482640.2 and CR434700.1. On Oct 6, 2011 this sequence version replaced NM_001126979.1. ##Evidence-Data-START## Transcript exon combination :: BC161232.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGCTC CTGTTTCTGA TTTGAAGATG GAAACCTGCG CGAATGAAAA CATCTGCATC 
TCTGAAGAAA TAAGTCAAGA AGGTTCCCCA GCAGAGGAAG ATGGAAGCAA TGAAATGGCA 
AAATCTTCTG CTGAGTCCTG TCCAGACAGC AAGAATAAAG CAGGGATTTA TAGATACATC 
AAAGACGATC TCTTCACTTC AGAGATTTTC AAAGTAGAAA TACAAAACTT GCCAAAATAC 
ATATGCTTTA ATGACTTAAA AAAATTCCTT GGCAAGTATG GGCTAAATCC TCATAAGATT 
AAACTCATGA ATAAACAAAC TTTTGCATTT GTGACTTTTA AAAGTGAAGA AGAGCGTGAC 
AAAGCTATGA AAGTCATACA TGGGGTGAAG TGGAAGAACC GTACACTTAG TGTTCGACTT 
GCAAAGCCCA GGGCTGACCC CATAATGCAA AAAAGAAAGC AGGAAGAAGG AGATGGTGAA 
CAGCCAGAAA GCAAGCGTCA GGCTGCACAC AATGATGCAG AGGAGGAGCC CCTCAGTAAA 
ACGATCTCAG ATGTAGTGAC TCCTTTGTGG CAAGTTCCAT ATGAAGAACA GCTGAAAATT 
AAGGAGGAAG AATGTTGCAA AGTATTGAAG CAGTTAACAA AGGAAATAGG AGTGAATAAC 
AAGCCACTAG CCCCTTGGCT GTTTGAGCAG AAACAGAAGT ACAATAGACT CTGCTGTCCC 
CTGGAACGTG TCATACCATC TCCAATACAG ACAGAGTATC GTAACAAATC TGAATTCCTT 
ATTGGATATG GTGTTAACCA GGAAGACAAA ACAGTGGGCT GCAGGCTTAG CAAATATAAA 
GGAGGCACAT GCGCTGTGGT TGAGCCATTT GATACAATAA ACATTCCCCC TGTTACCAAG 
AATGTTGTGA GAGCTTTCCA GGAATACATA AGGAGCACTC CATATGATGT CTATAGCCCT 
GAAACATATG CCGGTCACTG GAAGCAGCTT ACTGTCCGTA CCAGCAGAAA GGGGCAGGTT 
ATGGCAATAG TGTATTTTAA CCCCCAGAAA CTGCCTAAGG AGGATCTCTC AGAGCTAAAA 
ACGTCACTAT CAATGTATTT CACTGAAGGG TCGGGGAAGG AAAGTGGTGT CACCTCCCTT 
TATTTTGTTG AAGAGGGTCA AAGGAAGTCT CCCCACCTGG AGGATTTGCC TGTGGAACAT 
ATAGCTGGAG ACCAGTATAT CTATGAAGAT CTACTTGGTC TAACCTTCAG AATTTCACCT 
CATGCCTTCT TCCAGGTTAA TACACCTGCA GCTGAGGTTC TGTATTCAGC CATTGCAGAC 
TGGGCTCAGC TGGATCAGAA CACCACTGTT TTGGATGTTT GCTGTGGGAC TGGAACCATT 
GGCATTTCTT TGGCTAAGAA AGTCAAAAAA GTTGTTGGAA TTGAACTGTG TCAGGAGGCT 
ATTGCAGATG CCAAGGCAAA TGCTCAACTA AATAATTTGG ACAATGTAGA GTTCCGATGT 
GGCAAAGCTG AGGACATCTT TCCCACCCTT ATTTACTCTT TCACGTTCCC AAGCCCACTG 
GCCATTGTTG ATCCACCAAG AGCCGGCCTC CACTCCAAGG TGATTATTGC AATTAGGAAA 
GCAGAGCATT TGAAGCAACT GATATATGTT TCCTGCAATC CCAAGGCAGC AATGAACAAC 
TTTATTGACC TGTGCCGTGC TCCTTCCAAT CGTGTCAAGG GGAGACCATT TCGGCCAATC 
AAAGCCTTAG CTGTAGACCT TTTCCCGCAG ACTCCACATT TTGAATTGGT TATTCTGTTT 
GAGAGAACGG AACCTGCAGA AATCGTAACT GATCCGTCTA AAAGCACCGA GCCGTCACTT 
GTGCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001120451.2
CDS	11..1879
Translation	MAAPVSDLKMETCANENICISEEISQEGSPAEEDGSNEMAKSSAESCPDSKNKAGIYRYIKDDLFTSEIFKVEIQNLPKYICFNDLKKFLGKYGLNPHKIKLMNKQTFAFVTFKSEEERDKAMKVIHGVKWKNRTLSVRLAKPRADPIMQKRKQEEGDGEQPESKRQAAHNDAEEEPLSKTISDVVTPLWQVPYEEQLKIKEEECCKVLKQLTKEIGVNNKPLAPWLFEQKQKYNRLCCPLERVIPSPIQTEYRNKSEFLIGYGVNQEDKTVGCRLSKYKGGTCAVVEPFDTINIPPVTKNVVRAFQEYIRSTPYDVYSPETYAGHWKQLTVRTSRKGQVMAIVYFNPQKLPKEDLSELKTSLSMYFTEGSGKESGVTSLYFVEEGQRKSPHLEDLPVEHIAGDQYIYEDLLGLTFRISPHAFFQVNTPAAEVLYSAIADWAQLDQNTTVLDVCCGTGTIGISLAKKVKKVVGIELCQEAIADAKANAQLNNLDNVEFRCGKAEDIFPTLIYSFTFPSPLAIVDPPRAGLHSKVIIAIRKAEHLKQLIYVSCNPKAAMNNFIDLCRAPSNRVKGRPFRPIKALAVDLFPQTPHFELVILFERTEPAEIVTDPSKSTEPSLVP

Target ORF information:

RefSeq Version	NM_001126979.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tRNA methyltransferase 2 homolog A (trmt2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001126979.2

ORF Insert Sequence:

ATGGCAGCTC CTGTTTCTGA TTTGAAGATG GAAACCTGCG CGAATGAAAA CATCTGCATC 
TCTGAAGAAA TAAGTCAAGA AGGTTCCCCA GCAGAGGAAG ATGGAAGCAA TGAAATGGCA 
AAATCTTCTG CTGAGTCCTG TCCAGACAGC AAGAATAAAG CAGGGATTTA TAGATACATC 
AAAGACGATC TCTTCACTTC AGAGATTTTC AAAGTAGAAA TACAAAACTT GCCAAAATAC 
ATATGCTTTA ATGACTTAAA AAAATTCCTT GGCAAGTATG GGCTAAATCC TCATAAGATT 
AAACTCATGA ATAAACAAAC TTTTGCATTT GTGACTTTTA AAAGTGAAGA AGAGCGTGAC 
AAAGCTATGA AAGTCATACA TGGGGTGAAG TGGAAGAACC GTACACTTAG TGTTCGACTT 
GCAAAGCCCA GGGCTGACCC CATAATGCAA AAAAGAAAGC AGGAAGAAGG AGATGGTGAA 
CAGCCAGAAA GCAAGCGTCA GGCTGCACAC AATGATGCAG AGGAGGAGCC CCTCAGTAAA 
ACGATCTCAG ATGTAGTGAC TCCTTTGTGG CAAGTTCCAT ATGAAGAACA GCTGAAAATT 
AAGGAGGAAG AATGTTGCAA AGTATTGAAG CAGTTAACAA AGGAAATAGG AGTGAATAAC 
AAGCCACTAG CCCCTTGGCT GTTTGAGCAG AAACAGAAGT ACAATAGACT CTGCTGTCCC 
CTGGAACGTG TCATACCATC TCCAATACAG ACAGAGTATC GTAACAAATC TGAATTCCTT 
ATTGGATATG GTGTTAACCA GGAAGACAAA ACAGTGGGCT GCAGGCTTAG CAAATATAAA 
GGAGGCACAT GCGCTGTGGT TGAGCCATTT GATACAATAA ACATTCCCCC TGTTACCAAG 
AATGTTGTGA GAGCTTTCCA GGAATACATA AGGAGCACTC CATATGATGT CTATAGCCCT 
GAAACATATG CCGGTCACTG GAAGCAGCTT ACTGTCCGTA CCAGCAGAAA GGGGCAGGTT 
ATGGCAATAG TGTATTTTAA CCCCCAGAAA CTGCCTAAGG AGGATCTCTC AGAGCTAAAA 
ACGTCACTAT CAATGTATTT CACTGAAGGG TCGGGGAAGG AAAGTGGTGT CACCTCCCTT 
TATTTTGTTG AAGAGGGTCA AAGGAAGTCT CCCCACCTGG AGGATTTGCC TGTGGAACAT 
ATAGCTGGAG ACCAGTATAT CTATGAAGAT CTACTTGGTC TAACCTTCAG AATTTCACCT 
CATGCCTTCT TCCAGGTTAA TACACCTGCA GCTGAGGTTC TGTATTCAGC CATTGCAGAC 
TGGGCTCAGC TGGATCAGAA CACCACTGTT TTGGATGTTT GCTGTGGGAC TGGAACCATT 
GGCATTTCTT TGGCTAAGAA AGTCAAAAAA GTTGTTGGAA TTGAACTGTG TCAGGAGGCT 
ATTGCAGATG CCAAGGCAAA TGCTCAACTA AATAATTTGG ACAATGTAGA GTTCCGATGT 
GGCAAAGCTG AGGACATCTT TCCCACCCTT ATTTACTCTT TCACGTTCCC AAGCCCACTG 
GCCATTGTTG ATCCACCAAG AGCCGGCCTC CACTCCAAGG TGATTATTGC AATTAGGAAA 
GCAGAGCATT TGAAGCAACT GATATATGTT TCCTGCAATC CCAAGGCAGC AATGAACAAC 
TTTATTGACC TGTGCCGTGC TCCTTCCAAT CGTGTCAAGG GGAGACCATT TCGGCCAATC 
AAAGCCTTAG CTGTAGACCT TTTCCCGCAG ACTCCACATT TTGAATTGGT TATTCTGTTT 
GAGAGAACGG AACCTGCAGA AATCGTAACT GATCCGTCTA AAAGCACCGA GCCGTCACTT 
GTGCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trmt2a ( XM_012956240.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811694.1 Xenopus tropicalis tRNA methyltransferase 2 homolog A (trmt2a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa31888
Clone ID Related Accession (Same CDS sequence)	XM_012956240.1
Accession Version	XM_012956240.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tRNA (uracil-5-)-methyltransferase homolog A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGTTT CCAGTTTCCG TTTACGTCAC CAGCATGGCA GCTCCTGTAT GGAAACCTGC 
GCGAATGAAA ACATCTGCAT CTCTGAAGAA ATAAGTCAAG AAGGTTCCCC AGCAGAGGAA 
GATGGAAGCA ATGAAATGGC AAAATCTTCT GCTGAGTCCT GTCCAGACAG CAAGAATAAA 
GCAGGGATTT ATAGATACAT CAAAGACGAT CTCTTCACTT CAGAGATTTT CAAAGTAGAA 
ATACAAAACT TGCCAAAATA CATATGCTTT AATGACTTAA AAAAATTCCT TGGCAAGTAT 
GGGCTAAATC CTCATAAGAT TAAACTCATG AATAAACAAA CTTTTGCATT TGTGACTTTT 
AAAAGTGAAG AAGAGCGTGA CAAAGCTATG AAAGTCATAC ATGGGGTGAA GTGGAAGAAC 
CGTACACTTA GTGTTCGACT TGCAAAGCCC AGGGCTGACC CCATAATGCA AAAAAGAAAG 
CAGGAAGAAG GAGATGGTGA ACAGCCAGAA AGCAAGCGTC AGGCTGCACA CAATGATGCA 
GAGGAGGAGC CCCTCAGTAA AACGATCTCA GATGTAGTGA CTCCTTTGTG GCAAGTTCCA 
TATGAAGAAC AGCTGAAAAT TAAGGAGGAA GAATGTTGCA AAGTATTGAA GCAGTTAACA 
AAGGAAATAG GAGTGAATAA CAAGCCACTA GCCCCTTGGC TGTTTGAGCA GAAACAGAAG 
TACAATAGAC TCTGCTGTCC CCTGGAACGT GTCATACCAT CTCCAATACA GACAGAGTAT 
CGTAACAAAT CTGAATTCCT TATTGGATAT GGTGTTAACC AGGAAGACAA AACAGTGGGC 
TGCAGGCTTA GCAAATATAA AGGAGGCACA TGCGCTGTGG TTGAGCCATT TGATACAATA 
AACATTCCCC CTGTTACCAA GAATGTTGTG AGAGCTTTCC AGGAATACAT AAGGAGCACT 
CCATATGATG TCTATAGCCC TGAAACATAT GCCGGTCACT GGAAGCAGCT TACTGTCCGT 
ACCAGCAGAA AGGGGCAGGT TATGGCAATA GTGTATTTTA ACCCCCAGAA ACTGCCTAAG 
GAGGATCTCT CAGAGCTAAA AACGTCACTA TCAATGTATT TCACTGAAGG GTCGGGGAAG 
GAAAGTGGTG TCACCTCCCT TTATTTTGTT GAAGAGGGTC AAAGGAAGTC TCCCCACCTG 
GAGGATTTGC CTGTGGAACA TATAGCTGGA GACCAGTATA TCTATGAAGA TCTACTTGGT 
CTAACCTTCA GAATTTCACC TCATGCCTTC TTCCAGGTTA ATACACCTGC AGCTGAGGTT 
CTGTATTCAG CCATTGCAGA CTGGGCTCAG CTGGATCAGA ACACCACTGT TTTGGATGTT 
TGCTGTGGGA CTGGAACCAT TGGCATTTCT TTGGCTAAGA AAGTCAAAAA AGTTGTTGGA 
ATTGAACTGT GTCAGGAGGC TATTGCAGAT GCCAAGGCAA ATGCTCAACT AAATAATTTG 
GACAATGTAG AGTTCCGATG TGGCAAAGCT GAGGACATCT TTCCCACCCT TATTTACTCT 
TTCACGTTCC CAAGCCCACT GGCCATTGTT GATCCACCAA GAGCCGGCCT CCACTCCAAG 
GTGATTATTG CAATTAGGAA AGCAGAGCAT TTGAAGCAAC TGATATATGT TTCCTGCAAT 
CCCAAGGCAG CAATGAACAA CTTTATTGAC CTGTGCCGTG CTCCTTCCAA TCGTGTCAAG 
GGGAGACCAT TTCGGCCAAT CAAAGCCTTA GCTGTAGACC TTTTCCCGCA GACTCCACAT 
TTTGAATTGG TTATTCTGTT TGAGAGAACG GAACCTGCAG AAATCGTAAC TGATCCGTCT 
AAAAGCACCG AGCCGTCACT TGTGCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811694.1
CDS	36..1925
Translation	MPVSSFRLRHQHGSSCMETCANENICISEEISQEGSPAEEDGSNEMAKSSAESCPDSKNKAGIYRYIKDDLFTSEIFKVEIQNLPKYICFNDLKKFLGKYGLNPHKIKLMNKQTFAFVTFKSEEERDKAMKVIHGVKWKNRTLSVRLAKPRADPIMQKRKQEEGDGEQPESKRQAAHNDAEEEPLSKTISDVVTPLWQVPYEEQLKIKEEECCKVLKQLTKEIGVNNKPLAPWLFEQKQKYNRLCCPLERVIPSPIQTEYRNKSEFLIGYGVNQEDKTVGCRLSKYKGGTCAVVEPFDTINIPPVTKNVVRAFQEYIRSTPYDVYSPETYAGHWKQLTVRTSRKGQVMAIVYFNPQKLPKEDLSELKTSLSMYFTEGSGKESGVTSLYFVEEGQRKSPHLEDLPVEHIAGDQYIYEDLLGLTFRISPHAFFQVNTPAAEVLYSAIADWAQLDQNTTVLDVCCGTGTIGISLAKKVKKVVGIELCQEAIADAKANAQLNNLDNVEFRCGKAEDIFPTLIYSFTFPSPLAIVDPPRAGLHSKVIIAIRKAEHLKQLIYVSCNPKAAMNNFIDLCRAPSNRVKGRPFRPIKALAVDLFPQTPHFELVILFERTEPAEIVTDPSKSTEPSLVP

Target ORF information:

RefSeq Version	XM_012956240.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tRNA methyltransferase 2 homolog A (trmt2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956240.1

ORF Insert Sequence:

ATGCCAGTTT CCAGTTTCCG TTTACGTCAC CAGCATGGCA GCTCCTGTAT GGAAACCTGC 
GCGAATGAAA ACATCTGCAT CTCTGAAGAA ATAAGTCAAG AAGGTTCCCC AGCAGAGGAA 
GATGGAAGCA ATGAAATGGC AAAATCTTCT GCTGAGTCCT GTCCAGACAG CAAGAATAAA 
GCAGGGATTT ATAGATACAT CAAAGACGAT CTCTTCACTT CAGAGATTTT CAAAGTAGAA 
ATACAAAACT TGCCAAAATA CATATGCTTT AATGACTTAA AAAAATTCCT TGGCAAGTAT 
GGGCTAAATC CTCATAAGAT TAAACTCATG AATAAACAAA CTTTTGCATT TGTGACTTTT 
AAAAGTGAAG AAGAGCGTGA CAAAGCTATG AAAGTCATAC ATGGGGTGAA GTGGAAGAAC 
CGTACACTTA GTGTTCGACT TGCAAAGCCC AGGGCTGACC CCATAATGCA AAAAAGAAAG 
CAGGAAGAAG GAGATGGTGA ACAGCCAGAA AGCAAGCGTC AGGCTGCACA CAATGATGCA 
GAGGAGGAGC CCCTCAGTAA AACGATCTCA GATGTAGTGA CTCCTTTGTG GCAAGTTCCA 
TATGAAGAAC AGCTGAAAAT TAAGGAGGAA GAATGTTGCA AAGTATTGAA GCAGTTAACA 
AAGGAAATAG GAGTGAATAA CAAGCCACTA GCCCCTTGGC TGTTTGAGCA GAAACAGAAG 
TACAATAGAC TCTGCTGTCC CCTGGAACGT GTCATACCAT CTCCAATACA GACAGAGTAT 
CGTAACAAAT CTGAATTCCT TATTGGATAT GGTGTTAACC AGGAAGACAA AACAGTGGGC 
TGCAGGCTTA GCAAATATAA AGGAGGCACA TGCGCTGTGG TTGAGCCATT TGATACAATA 
AACATTCCCC CTGTTACCAA GAATGTTGTG AGAGCTTTCC AGGAATACAT AAGGAGCACT 
CCATATGATG TCTATAGCCC TGAAACATAT GCCGGTCACT GGAAGCAGCT TACTGTCCGT 
ACCAGCAGAA AGGGGCAGGT TATGGCAATA GTGTATTTTA ACCCCCAGAA ACTGCCTAAG 
GAGGATCTCT CAGAGCTAAA AACGTCACTA TCAATGTATT TCACTGAAGG GTCGGGGAAG 
GAAAGTGGTG TCACCTCCCT TTATTTTGTT GAAGAGGGTC AAAGGAAGTC TCCCCACCTG 
GAGGATTTGC CTGTGGAACA TATAGCTGGA GACCAGTATA TCTATGAAGA TCTACTTGGT 
CTAACCTTCA GAATTTCACC TCATGCCTTC TTCCAGGTTA ATACACCTGC AGCTGAGGTT 
CTGTATTCAG CCATTGCAGA CTGGGCTCAG CTGGATCAGA ACACCACTGT TTTGGATGTT 
TGCTGTGGGA CTGGAACCAT TGGCATTTCT TTGGCTAAGA AAGTCAAAAA AGTTGTTGGA 
ATTGAACTGT GTCAGGAGGC TATTGCAGAT GCCAAGGCAA ATGCTCAACT AAATAATTTG 
GACAATGTAG AGTTCCGATG TGGCAAAGCT GAGGACATCT TTCCCACCCT TATTTACTCT 
TTCACGTTCC CAAGCCCACT GGCCATTGTT GATCCACCAA GAGCCGGCCT CCACTCCAAG 
GTGATTATTG CAATTAGGAA AGCAGAGCAT TTGAAGCAAC TGATATATGT TTCCTGCAAT 
CCCAAGGCAG CAATGAACAA CTTTATTGAC CTGTGCCGTG CTCCTTCCAA TCGTGTCAAG 
GGGAGACCAT TTCGGCCAAT CAAAGCCTTA GCTGTAGACC TTTTCCCGCA GACTCCACAT 
TTTGAATTGG TTATTCTGTT TGAGAGAACG GAACCTGCAG AAATCGTAAC TGATCCGTCT 
AAAAGCACCG AGCCGTCACT TGTGCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trmt2a ( XM_012956246.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811700.1 Xenopus tropicalis tRNA methyltransferase 2 homolog A (trmt2a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa31889
Clone ID Related Accession (Same CDS sequence)	XM_012956246.2
Accession Version	XM_012956246.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tRNA (uracil-5-)-methyltransferase homolog A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012956246.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGATGT ATTCTGATTT GAAGATGGAA ACCTGCGCGA ATGAAAACAT CTGCATCTCT 
GAAGAAATAA GTCAAGAAGG TTCCCCAGCA GAGGAAGATG GAAGCAATGA AATGGCAAAA 
TCTTCTGCTG AGTCCTGTCC AGACAGCAAG AATAAAGCAG GGATTTATAG ATACATCAAA 
GACGATCTCT TCACTTCAGA GATTTTCAAA GTAGAAATAC AAAACTTGCC AAAATACATA 
TGCTTTAATG ACTTAAAAAA ATTCCTTGGC AAGTATGGGC TAAATCCTCA TAAGATTAAA 
CTCATGAATA AACAAACTTT TGCATTTGTG ACTTTTAAAA GTGAAGAAGA GCGTGACAAA 
GCTATGAAAG TCATACATGG GGTGAAGTGG AAGAACCGTA CACTTAGTGT TCGACTTGCA 
AAGCCCAGGG CTGACCCCAT AATGCAAAAA AGAAAGCAGG AAGAAGGAGA TGGTGAACAG 
CCAGAAAGCA AGCGTCAGGC TGCACACAAT GATGCAGAGG AGGAGCCCCT CAGTAAAACG 
ATCTCAGATG TAGTGACTCC TTTGTGGCAA GTTCCATATG AAGAACAGCT GAAAATTAAG 
GAGGAAGAAT GTTGCAAAGT ATTGAAGCAG TTAACAAAGG AAATAGGAGT GAATAACAAG 
CCACTAGCCC CTTGGCTGTT TGAGCAGAAA CAGAAGTACA ATAGACTCTG CTGTCCCCTG 
GAACGTGTCA TACCATCTCC AATACAGACA GAGTATCGTA ACAAATCTGA ATTCCTTATT 
GGATATGGTG TTAACCAGGA AGACAAAACA GTGGGCTGCA GGCTTAGCAA ATATAAAGGA 
GGCACATGCG CTGTGGTTGA GCCATTTGAT ACAATAAACA TTCCCCCTGT TACCAAGAAT 
GTTGTGAGAG CTTTCCAGGA ATACATAAGG AGCACTCCAT ATGATGTCTA TAGCCCTGAA 
ACATATGCCG GTCACTGGAA GCAGCTTACT GTCCGTACCA GCAGAAAGGG GCAGGTTATG 
GCAATAGTGT ATTTTAACCC CCAGAAACTG CCTAAGGAGG ATCTCTCAGA GCTAAAAACG 
TCACTATCAA TGTATTTCAC TGAAGGGTCG GGGAAGGAAA GTGGTGTCAC CTCCCTTTAT 
TTTGTTGAAG AGGGTCAAAG GAAGTCTCCC CACCTGGAGG ATTTGCCTGT GGAACATATA 
GCTGGAGACC AGTATATCTA TGAAGATCTA CTTGGTCTAA CCTTCAGAAT TTCACCTCAT 
GCCTTCTTCC AGGTTAATAC ACCTGCAGCT GAGGTTCTGT ATTCAGCCAT TGCAGACTGG 
GCTCAGCTGG ATCAGAACAC CACTGTTTTG GATGTTTGCT GTGGGACTGG AACCATTGGC 
ATTTCTTTGG CTAAGAAAGT CAAAAAAGTT GTTGGAATTG AACTGTGTCA GGAGGCTATT 
GCAGATGCCA AGGCAAATGC TCAACTAAAT AATTTGGACA ATGTAGAGTT CCGATGTGGC 
AAAGCTGAGG ACATCTTTCC CACCCTTATT TACTCTTTCA CGTTCCCAAG CCCACTGGCC 
ATTGTTGATC CACCAAGAGC CGGCCTCCAC TCCAAGGTGA TTATTGCAAT TAGGAAAGCA 
GAGCATTTGA AGCAACTGAT ATATGTTTCC TGCAATCCCA AGGCAGCAAT GAACAACTTT 
ATTGACCTGT GCCGTGCTCC TTCCAATCGT GTCAAGGGGA GACCATTTCG GCCAATCAAA 
GCCTTAGCTG TAGACCTTTT CCCGCAGACT CCACATTTTG AATTGGTTAT TCTGTTTGAG 
AGAACGGAAC CTGCAGAAAT CGTAACTGAT CCGTCTAAAA GCACCGAGCC GTCACTTGTG 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811700.1
CDS	46..1911
Translation	MLMYSDLKMETCANENICISEEISQEGSPAEEDGSNEMAKSSAESCPDSKNKAGIYRYIKDDLFTSEIFKVEIQNLPKYICFNDLKKFLGKYGLNPHKIKLMNKQTFAFVTFKSEEERDKAMKVIHGVKWKNRTLSVRLAKPRADPIMQKRKQEEGDGEQPESKRQAAHNDAEEEPLSKTISDVVTPLWQVPYEEQLKIKEEECCKVLKQLTKEIGVNNKPLAPWLFEQKQKYNRLCCPLERVIPSPIQTEYRNKSEFLIGYGVNQEDKTVGCRLSKYKGGTCAVVEPFDTINIPPVTKNVVRAFQEYIRSTPYDVYSPETYAGHWKQLTVRTSRKGQVMAIVYFNPQKLPKEDLSELKTSLSMYFTEGSGKESGVTSLYFVEEGQRKSPHLEDLPVEHIAGDQYIYEDLLGLTFRISPHAFFQVNTPAAEVLYSAIADWAQLDQNTTVLDVCCGTGTIGISLAKKVKKVVGIELCQEAIADAKANAQLNNLDNVEFRCGKAEDIFPTLIYSFTFPSPLAIVDPPRAGLHSKVIIAIRKAEHLKQLIYVSCNPKAAMNNFIDLCRAPSNRVKGRPFRPIKALAVDLFPQTPHFELVILFERTEPAEIVTDPSKSTEPSLVP

Target ORF information:

RefSeq Version	XM_012956246.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tRNA methyltransferase 2 homolog A (trmt2a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956246.2

ORF Insert Sequence:

ATGCTGATGT ATTCTGATTT GAAGATGGAA ACCTGCGCGA ATGAAAACAT CTGCATCTCT 
GAAGAAATAA GTCAAGAAGG TTCCCCAGCA GAGGAAGATG GAAGCAATGA AATGGCAAAA 
TCTTCTGCTG AGTCCTGTCC AGACAGCAAG AATAAAGCAG GGATTTATAG ATACATCAAA 
GACGATCTCT TCACTTCAGA GATTTTCAAA GTAGAAATAC AAAACTTGCC AAAATACATA 
TGCTTTAATG ACTTAAAAAA ATTCCTTGGC AAGTATGGGC TAAATCCTCA TAAGATTAAA 
CTCATGAATA AACAAACTTT TGCATTTGTG ACTTTTAAAA GTGAAGAAGA GCGTGACAAA 
GCTATGAAAG TCATACATGG GGTGAAGTGG AAGAACCGTA CACTTAGTGT TCGACTTGCA 
AAGCCCAGGG CTGACCCCAT AATGCAAAAA AGAAAGCAGG AAGAAGGAGA TGGTGAACAG 
CCAGAAAGCA AGCGTCAGGC TGCACACAAT GATGCAGAGG AGGAGCCCCT CAGTAAAACG 
ATCTCAGATG TAGTGACTCC TTTGTGGCAA GTTCCATATG AAGAACAGCT GAAAATTAAG 
GAGGAAGAAT GTTGCAAAGT ATTGAAGCAG TTAACAAAGG AAATAGGAGT GAATAACAAG 
CCACTAGCCC CTTGGCTGTT TGAGCAGAAA CAGAAGTACA ATAGACTCTG CTGTCCCCTG 
GAACGTGTCA TACCATCTCC AATACAGACA GAGTATCGTA ACAAATCTGA ATTCCTTATT 
GGATATGGTG TTAACCAGGA AGACAAAACA GTGGGCTGCA GGCTTAGCAA ATATAAAGGA 
GGCACATGCG CTGTGGTTGA GCCATTTGAT ACAATAAACA TTCCCCCTGT TACCAAGAAT 
GTTGTGAGAG CTTTCCAGGA ATACATAAGG AGCACTCCAT ATGATGTCTA TAGCCCTGAA 
ACATATGCCG GTCACTGGAA GCAGCTTACT GTCCGTACCA GCAGAAAGGG GCAGGTTATG 
GCAATAGTGT ATTTTAACCC CCAGAAACTG CCTAAGGAGG ATCTCTCAGA GCTAAAAACG 
TCACTATCAA TGTATTTCAC TGAAGGGTCG GGGAAGGAAA GTGGTGTCAC CTCCCTTTAT 
TTTGTTGAAG AGGGTCAAAG GAAGTCTCCC CACCTGGAGG ATTTGCCTGT GGAACATATA 
GCTGGAGACC AGTATATCTA TGAAGATCTA CTTGGTCTAA CCTTCAGAAT TTCACCTCAT 
GCCTTCTTCC AGGTTAATAC ACCTGCAGCT GAGGTTCTGT ATTCAGCCAT TGCAGACTGG 
GCTCAGCTGG ATCAGAACAC CACTGTTTTG GATGTTTGCT GTGGGACTGG AACCATTGGC 
ATTTCTTTGG CTAAGAAAGT CAAAAAAGTT GTTGGAATTG AACTGTGTCA GGAGGCTATT 
GCAGATGCCA AGGCAAATGC TCAACTAAAT AATTTGGACA ATGTAGAGTT CCGATGTGGC 
AAAGCTGAGG ACATCTTTCC CACCCTTATT TACTCTTTCA CGTTCCCAAG CCCACTGGCC 
ATTGTTGATC CACCAAGAGC CGGCCTCCAC TCCAAGGTGA TTATTGCAAT TAGGAAAGCA 
GAGCATTTGA AGCAACTGAT ATATGTTTCC TGCAATCCCA AGGCAGCAAT GAACAACTTT 
ATTGACCTGT GCCGTGCTCC TTCCAATCGT GTCAAGGGGA GACCATTTCG GCCAATCAAA 
GCCTTAGCTG TAGACCTTTT CCCGCAGACT CCACATTTTG AATTGGTTAT TCTGTTTGAG 
AGAACGGAAC CTGCAGAAAT CGTAACTGAT CCGTCTAAAA GCACCGAGCC GTCACTTGTG 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trmt2a ( XM_012956249.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811703.1 Xenopus tropicalis tRNA methyltransferase 2 homolog A (trmt2a), transcript variant X3, mRNA.

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CloneID	OXa31890
Clone ID Related Accession (Same CDS sequence)	XM_012956249.2
Accession Version	XM_012956249.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1863bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tRNA (uracil-5-)-methyltransferase homolog A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012956249.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGATTT CTGATTTGAA GATGGAAACC TGCGCGAATG AAAACATCTG CATCTCTGAA 
GAAATAAGTC AAGAAGGTTC CCCAGCAGAG GAAGATGGAA GCAATGAAAT GGCAAAATCT 
TCTGCTGAGT CCTGTCCAGA CAGCAAGAAT AAAGCAGGGA TTTATAGATA CATCAAAGAC 
GATCTCTTCA CTTCAGAGAT TTTCAAAGTA GAAATACAAA ACTTGCCAAA ATACATATGC 
TTTAATGACT TAAAAAAATT CCTTGGCAAG TATGGGCTAA ATCCTCATAA GATTAAACTC 
ATGAATAAAC AAACTTTTGC ATTTGTGACT TTTAAAAGTG AAGAAGAGCG TGACAAAGCT 
ATGAAAGTCA TACATGGGGT GAAGTGGAAG AACCGTACAC TTAGTGTTCG ACTTGCAAAG 
CCCAGGGCTG ACCCCATAAT GCAAAAAAGA AAGCAGGAAG AAGGAGATGG TGAACAGCCA 
GAAAGCAAGC GTCAGGCTGC ACACAATGAT GCAGAGGAGG AGCCCCTCAG TAAAACGATC 
TCAGATGTAG TGACTCCTTT GTGGCAAGTT CCATATGAAG AACAGCTGAA AATTAAGGAG 
GAAGAATGTT GCAAAGTATT GAAGCAGTTA ACAAAGGAAA TAGGAGTGAA TAACAAGCCA 
CTAGCCCCTT GGCTGTTTGA GCAGAAACAG AAGTACAATA GACTCTGCTG TCCCCTGGAA 
CGTGTCATAC CATCTCCAAT ACAGACAGAG TATCGTAACA AATCTGAATT CCTTATTGGA 
TATGGTGTTA ACCAGGAAGA CAAAACAGTG GGCTGCAGGC TTAGCAAATA TAAAGGAGGC 
ACATGCGCTG TGGTTGAGCC ATTTGATACA ATAAACATTC CCCCTGTTAC CAAGAATGTT 
GTGAGAGCTT TCCAGGAATA CATAAGGAGC ACTCCATATG ATGTCTATAG CCCTGAAACA 
TATGCCGGTC ACTGGAAGCA GCTTACTGTC CGTACCAGCA GAAAGGGGCA GGTTATGGCA 
ATAGTGTATT TTAACCCCCA GAAACTGCCT AAGGAGGATC TCTCAGAGCT AAAAACGTCA 
CTATCAATGT ATTTCACTGA AGGGTCGGGG AAGGAAAGTG GTGTCACCTC CCTTTATTTT 
GTTGAAGAGG GTCAAAGGAA GTCTCCCCAC CTGGAGGATT TGCCTGTGGA ACATATAGCT 
GGAGACCAGT ATATCTATGA AGATCTACTT GGTCTAACCT TCAGAATTTC ACCTCATGCC 
TTCTTCCAGG TTAATACACC TGCAGCTGAG GTTCTGTATT CAGCCATTGC AGACTGGGCT 
CAGCTGGATC AGAACACCAC TGTTTTGGAT GTTTGCTGTG GGACTGGAAC CATTGGCATT 
TCTTTGGCTA AGAAAGTCAA AAAAGTTGTT GGAATTGAAC TGTGTCAGGA GGCTATTGCA 
GATGCCAAGG CAAATGCTCA ACTAAATAAT TTGGACAATG TAGAGTTCCG ATGTGGCAAA 
GCTGAGGACA TCTTTCCCAC CCTTATTTAC TCTTTCACGT TCCCAAGCCC ACTGGCCATT 
GTTGATCCAC CAAGAGCCGG CCTCCACTCC AAGGTGATTA TTGCAATTAG GAAAGCAGAG 
CATTTGAAGC AACTGATATA TGTTTCCTGC AATCCCAAGG CAGCAATGAA CAACTTTATT 
GACCTGTGCC GTGCTCCTTC CAATCGTGTC AAGGGGAGAC CATTTCGGCC AATCAAAGCC 
TTAGCTGTAG ACCTTTTCCC GCAGACTCCA CATTTTGAAT TGGTTATTCT GTTTGAGAGA 
ACGGAACCTG CAGAAATCGT AACTGATCCG TCTAAAAGCA CCGAGCCGTC ACTTGTGCCG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811703.1
CDS	71..1933
Translation	MLISDLKMETCANENICISEEISQEGSPAEEDGSNEMAKSSAESCPDSKNKAGIYRYIKDDLFTSEIFKVEIQNLPKYICFNDLKKFLGKYGLNPHKIKLMNKQTFAFVTFKSEEERDKAMKVIHGVKWKNRTLSVRLAKPRADPIMQKRKQEEGDGEQPESKRQAAHNDAEEEPLSKTISDVVTPLWQVPYEEQLKIKEEECCKVLKQLTKEIGVNNKPLAPWLFEQKQKYNRLCCPLERVIPSPIQTEYRNKSEFLIGYGVNQEDKTVGCRLSKYKGGTCAVVEPFDTINIPPVTKNVVRAFQEYIRSTPYDVYSPETYAGHWKQLTVRTSRKGQVMAIVYFNPQKLPKEDLSELKTSLSMYFTEGSGKESGVTSLYFVEEGQRKSPHLEDLPVEHIAGDQYIYEDLLGLTFRISPHAFFQVNTPAAEVLYSAIADWAQLDQNTTVLDVCCGTGTIGISLAKKVKKVVGIELCQEAIADAKANAQLNNLDNVEFRCGKAEDIFPTLIYSFTFPSPLAIVDPPRAGLHSKVIIAIRKAEHLKQLIYVSCNPKAAMNNFIDLCRAPSNRVKGRPFRPIKALAVDLFPQTPHFELVILFERTEPAEIVTDPSKSTEPSLVP

Target ORF information:

RefSeq Version	XM_012956249.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tRNA methyltransferase 2 homolog A (trmt2a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956249.2

ORF Insert Sequence:

ATGCTGATTT CTGATTTGAA GATGGAAACC TGCGCGAATG AAAACATCTG CATCTCTGAA 
GAAATAAGTC AAGAAGGTTC CCCAGCAGAG GAAGATGGAA GCAATGAAAT GGCAAAATCT 
TCTGCTGAGT CCTGTCCAGA CAGCAAGAAT AAAGCAGGGA TTTATAGATA CATCAAAGAC 
GATCTCTTCA CTTCAGAGAT TTTCAAAGTA GAAATACAAA ACTTGCCAAA ATACATATGC 
TTTAATGACT TAAAAAAATT CCTTGGCAAG TATGGGCTAA ATCCTCATAA GATTAAACTC 
ATGAATAAAC AAACTTTTGC ATTTGTGACT TTTAAAAGTG AAGAAGAGCG TGACAAAGCT 
ATGAAAGTCA TACATGGGGT GAAGTGGAAG AACCGTACAC TTAGTGTTCG ACTTGCAAAG 
CCCAGGGCTG ACCCCATAAT GCAAAAAAGA AAGCAGGAAG AAGGAGATGG TGAACAGCCA 
GAAAGCAAGC GTCAGGCTGC ACACAATGAT GCAGAGGAGG AGCCCCTCAG TAAAACGATC 
TCAGATGTAG TGACTCCTTT GTGGCAAGTT CCATATGAAG AACAGCTGAA AATTAAGGAG 
GAAGAATGTT GCAAAGTATT GAAGCAGTTA ACAAAGGAAA TAGGAGTGAA TAACAAGCCA 
CTAGCCCCTT GGCTGTTTGA GCAGAAACAG AAGTACAATA GACTCTGCTG TCCCCTGGAA 
CGTGTCATAC CATCTCCAAT ACAGACAGAG TATCGTAACA AATCTGAATT CCTTATTGGA 
TATGGTGTTA ACCAGGAAGA CAAAACAGTG GGCTGCAGGC TTAGCAAATA TAAAGGAGGC 
ACATGCGCTG TGGTTGAGCC ATTTGATACA ATAAACATTC CCCCTGTTAC CAAGAATGTT 
GTGAGAGCTT TCCAGGAATA CATAAGGAGC ACTCCATATG ATGTCTATAG CCCTGAAACA 
TATGCCGGTC ACTGGAAGCA GCTTACTGTC CGTACCAGCA GAAAGGGGCA GGTTATGGCA 
ATAGTGTATT TTAACCCCCA GAAACTGCCT AAGGAGGATC TCTCAGAGCT AAAAACGTCA 
CTATCAATGT ATTTCACTGA AGGGTCGGGG AAGGAAAGTG GTGTCACCTC CCTTTATTTT 
GTTGAAGAGG GTCAAAGGAA GTCTCCCCAC CTGGAGGATT TGCCTGTGGA ACATATAGCT 
GGAGACCAGT ATATCTATGA AGATCTACTT GGTCTAACCT TCAGAATTTC ACCTCATGCC 
TTCTTCCAGG TTAATACACC TGCAGCTGAG GTTCTGTATT CAGCCATTGC AGACTGGGCT 
CAGCTGGATC AGAACACCAC TGTTTTGGAT GTTTGCTGTG GGACTGGAAC CATTGGCATT 
TCTTTGGCTA AGAAAGTCAA AAAAGTTGTT GGAATTGAAC TGTGTCAGGA GGCTATTGCA 
GATGCCAAGG CAAATGCTCA ACTAAATAAT TTGGACAATG TAGAGTTCCG ATGTGGCAAA 
GCTGAGGACA TCTTTCCCAC CCTTATTTAC TCTTTCACGT TCCCAAGCCC ACTGGCCATT 
GTTGATCCAC CAAGAGCCGG CCTCCACTCC AAGGTGATTA TTGCAATTAG GAAAGCAGAG 
CATTTGAAGC AACTGATATA TGTTTCCTGC AATCCCAAGG CAGCAATGAA CAACTTTATT 
GACCTGTGCC GTGCTCCTTC CAATCGTGTC AAGGGGAGAC CATTTCGGCC AATCAAAGCC 
TTAGCTGTAG ACCTTTTCCC GCAGACTCCA CATTTTGAAT TGGTTATTCT GTTTGAGAGA 
ACGGAACCTG CAGAAATCGT AACTGATCCG TCTAAAAGCA CCGAGCCGTC ACTTGTGCCG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trmt2a ( XM_012956240.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811694.2 Xenopus tropicalis tRNA methyltransferase 2 homolog A (trmt2a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa47583
Clone ID Related Accession (Same CDS sequence)	XM_012956240.2
Accession Version	XM_012956240.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tRNA (uracil-5-)-methyltransferase homolog A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012956240.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATG CCAGTTTCCG TTTACGTCAC CAGCATGGCA GCTCCTGTAT GGAAACCTGC 
GCGAATGAAA ACATCTGCAT CTCTGAAGAA ATAAGTCAAG AAGGTTCCCC AGCAGAGGAA 
GATGGAAGCA ATGAAATGGC AAAATCTTCT GCTGAGTCCT GTCCAGACAG CAAGAATAAA 
GCAGGGATTT ATAGATACAT CAAAGACGAT CTCTTCACTT CAGAGATTTT CAAAGTAGAA 
ATACAAAACT TGCCAAAATA CATATGCTTT AATGACTTAA AAAAATTCCT TGGCAAGTAT 
GGGCTAAATC CTCATAAGAT TAAACTCATG AATAAACAAA CTTTTGCATT TGTGACTTTT 
AAAAGTGAAG AAGAGCGTGA CAAAGCTATG AAAGTCATAC ATGGGGTGAA GTGGAAGAAC 
CGTACACTTA GTGTTCGACT TGCAAAGCCC AGGGCTGACC CCATAATGCA AAAAAGAAAG 
CAGGAAGAAG GAGATGGTGA ACAGCCAGAA AGCAAGCGTC AGGCTGCACA CAATGATGCA 
GAGGAGGAGC CCCTCAGTAA AACGATCTCG GATGTAGTGA CTCCTTTGTG GCAAGTTCCA 
TATGAAGAAC AGCTGAAAAT TAAGGAGGAA GAATGTTGCA AAGTATTGAA GCAGTTAACA 
AAGGAAATAG GAGTGAATAA CAAGCCACTA GCCCCTTGGC TGTTTGAGCA GAAACAGAAG 
TACAATAGAC TCTGCTGTCC CCTGGAACGT GTCATACCAT CTCCAATACA GACAGAGTAT 
CGTAACAAAT CTGAATTCCT TATTGGATAT GGTGTTAACC AGGAAGACAA AACAGTGGGC 
TGCAGGCTTA GCAAATATAA AGGAGGCACA TGCGCTGTGG TTGAGCCATT TGATACAATA 
AACATTCCCC CTGTTACCAA GAATGTTGTG AGAGCTTTCC AGGAATACAT AAGGAGCACT 
CCATATGATG TCTATAGCCC TGAAACATAT GCTGGTCACT GGAAGCAGCT TACTGTCCGT 
ACCAGCAGAA AGGGGCAGGT TATGGCAATA GTGTATTTTA ACCCCCAGAA ACTGCCTAAG 
GAGGATCTCT CAGAGCTAAA AACGTCACTA TCAATGTATT TCACTGAAGG GTCGGGGAAG 
GAAAGTGGTG TCACCTCCCT TTATTTTGTT GAAGAGGGTC AAAGGAAGTC TCCCCACCTG 
GAGGATTTGC CTGTGGAACA TATAGCTGGA GACCAGTATA TCTATGAAGA TCTACTTGGT 
CTAACCTTCA GAATTTCACC TCATGCCTTC TTCCAGGTTA ATACACCTGC AGCTGAGGTT 
CTGTATTCAG CCATTGCAGA CTGGGCTCAG CTGGATCAGA ACACCACTGT TTTGGATGTT 
TGCTGTGGGA CTGGAACCAT TGGCATTTCT TTGGCTAAGA AAGTCAAAAA AGTTGTTGGA 
ATTGAACTGT GTCAGGAGGC TATTGCAGAT GCCAAGGCAA ATGCTCAACT AAATAATTTG 
GACAATGTAG AGTTCCGATG TGGCAAAGCT GAGGACATCT TTCCCACCCT TATTTACTCT 
TTCACGTTCC CAAGCCCACT GGCCATTGTT GATCCACCAA GAGCCGGCCT CCACTCCAAG 
GTGATTATTG CAATTAGGAA AGCAGAGCAT TTGAAGCAAC TGATATATGT TTCCTGCAAT 
CCCAAGGCAG CAATGAACAA CTTTATTGAC CTGTGCCGTG CTCCTTCCAA TCGTGTCAAG 
GGGAGACCAT TTCGGCCAAT CAAAGCCTTA GCTGTAGACC TTTTCCCGCA GACTCCACAT 
TTTGAATTGG TTATTCTGTT TGAGAGAACG GAACCTGCAG AAATCGTAAC TGATCCGTCT 
AAAAGCACCG AGCCGTCACT TGTGCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811694.2
CDS	16..1905
Translation	MADASFRLRHQHGSSCMETCANENICISEEISQEGSPAEEDGSNEMAKSSAESCPDSKNKAGIYRYIKDDLFTSEIFKVEIQNLPKYICFNDLKKFLGKYGLNPHKIKLMNKQTFAFVTFKSEEERDKAMKVIHGVKWKNRTLSVRLAKPRADPIMQKRKQEEGDGEQPESKRQAAHNDAEEEPLSKTISDVVTPLWQVPYEEQLKIKEEECCKVLKQLTKEIGVNNKPLAPWLFEQKQKYNRLCCPLERVIPSPIQTEYRNKSEFLIGYGVNQEDKTVGCRLSKYKGGTCAVVEPFDTINIPPVTKNVVRAFQEYIRSTPYDVYSPETYAGHWKQLTVRTSRKGQVMAIVYFNPQKLPKEDLSELKTSLSMYFTEGSGKESGVTSLYFVEEGQRKSPHLEDLPVEHIAGDQYIYEDLLGLTFRISPHAFFQVNTPAAEVLYSAIADWAQLDQNTTVLDVCCGTGTIGISLAKKVKKVVGIELCQEAIADAKANAQLNNLDNVEFRCGKAEDIFPTLIYSFTFPSPLAIVDPPRAGLHSKVIIAIRKAEHLKQLIYVSCNPKAAMNNFIDLCRAPSNRVKGRPFRPIKALAVDLFPQTPHFELVILFERTEPAEIVTDPSKSTEPSLVP

Target ORF information:

RefSeq Version	XM_012956240.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tRNA methyltransferase 2 homolog A (trmt2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956240.2

ORF Insert Sequence:

ATGGCGGATG CCAGTTTCCG TTTACGTCAC CAGCATGGCA GCTCCTGTAT GGAAACCTGC 
GCGAATGAAA ACATCTGCAT CTCTGAAGAA ATAAGTCAAG AAGGTTCCCC AGCAGAGGAA 
GATGGAAGCA ATGAAATGGC AAAATCTTCT GCTGAGTCCT GTCCAGACAG CAAGAATAAA 
GCAGGGATTT ATAGATACAT CAAAGACGAT CTCTTCACTT CAGAGATTTT CAAAGTAGAA 
ATACAAAACT TGCCAAAATA CATATGCTTT AATGACTTAA AAAAATTCCT TGGCAAGTAT 
GGGCTAAATC CTCATAAGAT TAAACTCATG AATAAACAAA CTTTTGCATT TGTGACTTTT 
AAAAGTGAAG AAGAGCGTGA CAAAGCTATG AAAGTCATAC ATGGGGTGAA GTGGAAGAAC 
CGTACACTTA GTGTTCGACT TGCAAAGCCC AGGGCTGACC CCATAATGCA AAAAAGAAAG 
CAGGAAGAAG GAGATGGTGA ACAGCCAGAA AGCAAGCGTC AGGCTGCACA CAATGATGCA 
GAGGAGGAGC CCCTCAGTAA AACGATCTCG GATGTAGTGA CTCCTTTGTG GCAAGTTCCA 
TATGAAGAAC AGCTGAAAAT TAAGGAGGAA GAATGTTGCA AAGTATTGAA GCAGTTAACA 
AAGGAAATAG GAGTGAATAA CAAGCCACTA GCCCCTTGGC TGTTTGAGCA GAAACAGAAG 
TACAATAGAC TCTGCTGTCC CCTGGAACGT GTCATACCAT CTCCAATACA GACAGAGTAT 
CGTAACAAAT CTGAATTCCT TATTGGATAT GGTGTTAACC AGGAAGACAA AACAGTGGGC 
TGCAGGCTTA GCAAATATAA AGGAGGCACA TGCGCTGTGG TTGAGCCATT TGATACAATA 
AACATTCCCC CTGTTACCAA GAATGTTGTG AGAGCTTTCC AGGAATACAT AAGGAGCACT 
CCATATGATG TCTATAGCCC TGAAACATAT GCTGGTCACT GGAAGCAGCT TACTGTCCGT 
ACCAGCAGAA AGGGGCAGGT TATGGCAATA GTGTATTTTA ACCCCCAGAA ACTGCCTAAG 
GAGGATCTCT CAGAGCTAAA AACGTCACTA TCAATGTATT TCACTGAAGG GTCGGGGAAG 
GAAAGTGGTG TCACCTCCCT TTATTTTGTT GAAGAGGGTC AAAGGAAGTC TCCCCACCTG 
GAGGATTTGC CTGTGGAACA TATAGCTGGA GACCAGTATA TCTATGAAGA TCTACTTGGT 
CTAACCTTCA GAATTTCACC TCATGCCTTC TTCCAGGTTA ATACACCTGC AGCTGAGGTT 
CTGTATTCAG CCATTGCAGA CTGGGCTCAG CTGGATCAGA ACACCACTGT TTTGGATGTT 
TGCTGTGGGA CTGGAACCAT TGGCATTTCT TTGGCTAAGA AAGTCAAAAA AGTTGTTGGA 
ATTGAACTGT GTCAGGAGGC TATTGCAGAT GCCAAGGCAA ATGCTCAACT AAATAATTTG 
GACAATGTAG AGTTCCGATG TGGCAAAGCT GAGGACATCT TTCCCACCCT TATTTACTCT 
TTCACGTTCC CAAGCCCACT GGCCATTGTT GATCCACCAA GAGCCGGCCT CCACTCCAAG 
GTGATTATTG CAATTAGGAA AGCAGAGCAT TTGAAGCAAC TGATATATGT TTCCTGCAAT 
CCCAAGGCAG CAATGAACAA CTTTATTGAC CTGTGCCGTG CTCCTTCCAA TCGTGTCAAG 
GGGAGACCAT TTCGGCCAAT CAAAGCCTTA GCTGTAGACC TTTTCCCGCA GACTCCACAT 
TTTGAATTGG TTATTCTGTT TGAGAGAACG GAACCTGCAG AAATCGTAAC TGATCCGTCT 
AAAAGCACCG AGCCGTCACT TGTGCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trmt2a ( XM_012956249.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811703.1 Xenopus tropicalis tRNA methyltransferase 2 homolog A (trmt2a), transcript variant X2, mRNA.

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CloneID	OXa47584
Clone ID Related Accession (Same CDS sequence)	XM_012956249.3
Accession Version	XM_012956249.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1863bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tRNA (uracil-5-)-methyltransferase homolog A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012956249.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGATTT CTGATTTGAA GATGGAAACC TGCGCGAATG AAAACATCTG CATCTCTGAA 
GAAATAAGTC AAGAAGGTTC CCCAGCAGAG GAAGATGGAA GCAATGAAAT GGCAAAATCT 
TCTGCTGAGT CCTGTCCAGA CAGCAAGAAT AAAGCAGGGA TTTATAGATA CATCAAAGAC 
GATCTCTTCA CTTCAGAGAT TTTCAAAGTA GAAATACAAA ACTTGCCAAA ATACATATGC 
TTTAATGACT TAAAAAAATT CCTTGGCAAG TATGGGCTAA ATCCTCATAA GATTAAACTC 
ATGAATAAAC AAACTTTTGC ATTTGTGACT TTTAAAAGTG AAGAAGAGCG TGACAAAGCT 
ATGAAAGTCA TACATGGGGT GAAGTGGAAG AACCGTACAC TTAGTGTTCG ACTTGCAAAG 
CCCAGGGCTG ACCCCATAAT GCAAAAAAGA AAGCAGGAAG AAGGAGATGG TGAACAGCCA 
GAAAGCAAGC GTCAGGCTGC ACACAATGAT GCAGAGGAGG AGCCCCTCAG TAAAACGATC 
TCGGATGTAG TGACTCCTTT GTGGCAAGTT CCATATGAAG AACAGCTGAA AATTAAGGAG 
GAAGAATGTT GCAAAGTATT GAAGCAGTTA ACAAAGGAAA TAGGAGTGAA TAACAAGCCA 
CTAGCCCCTT GGCTGTTTGA GCAGAAACAG AAGTACAATA GACTCTGCTG TCCCCTGGAA 
CGTGTCATAC CATCTCCAAT ACAGACAGAG TATCGTAACA AATCTGAATT CCTTATTGGA 
TATGGTGTTA ACCAGGAAGA CAAAACAGTG GGCTGCAGGC TTAGCAAATA TAAAGGAGGC 
ACATGCGCTG TGGTTGAGCC ATTTGATACA ATAAACATTC CCCCTGTTAC CAAGAATGTT 
GTGAGAGCTT TCCAGGAATA CATAAGGAGC ACTCCATATG ATGTCTATAG CCCTGAAACA 
TATGCTGGTC ACTGGAAGCA GCTTACTGTC CGTACCAGCA GAAAGGGGCA GGTTATGGCA 
ATAGTGTATT TTAACCCCCA GAAACTGCCT AAGGAGGATC TCTCAGAGCT AAAAACGTCA 
CTATCAATGT ATTTCACTGA AGGGTCGGGG AAGGAAAGTG GTGTCACCTC CCTTTATTTT 
GTTGAAGAGG GTCAAAGGAA GTCTCCCCAC CTGGAGGATT TGCCTGTGGA ACATATAGCT 
GGAGACCAGT ATATCTATGA AGATCTACTT GGTCTAACCT TCAGAATTTC ACCTCATGCC 
TTCTTCCAGG TTAATACACC TGCAGCTGAG GTTCTGTATT CAGCCATTGC AGACTGGGCT 
CAGCTGGATC AGAACACCAC TGTTTTGGAT GTTTGCTGTG GGACTGGAAC CATTGGCATT 
TCTTTGGCTA AGAAAGTCAA AAAAGTTGTT GGAATTGAAC TGTGTCAGGA GGCTATTGCA 
GATGCCAAGG CAAATGCTCA ACTAAATAAT TTGGACAATG TAGAGTTCCG ATGTGGCAAA 
GCTGAGGACA TCTTTCCCAC CCTTATTTAC TCTTTCACGT TCCCAAGCCC ACTGGCCATT 
GTTGATCCAC CAAGAGCCGG CCTCCACTCC AAGGTGATTA TTGCAATTAG GAAAGCAGAG 
CATTTGAAGC AACTGATATA TGTTTCCTGC AATCCCAAGG CAGCAATGAA CAACTTTATT 
GACCTGTGCC GTGCTCCTTC CAATCGTGTC AAGGGGAGAC CATTTCGGCC AATCAAAGCC 
TTAGCTGTAG ACCTTTTCCC GCAGACTCCA CATTTTGAAT TGGTTATTCT GTTTGAGAGA 
ACGGAACCTG CAGAAATCGT AACTGATCCG TCTAAAAGCA CCGAGCCGTC ACTTGTGCCG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811703.1
CDS	16..1878
Translation	MLISDLKMETCANENICISEEISQEGSPAEEDGSNEMAKSSAESCPDSKNKAGIYRYIKDDLFTSEIFKVEIQNLPKYICFNDLKKFLGKYGLNPHKIKLMNKQTFAFVTFKSEEERDKAMKVIHGVKWKNRTLSVRLAKPRADPIMQKRKQEEGDGEQPESKRQAAHNDAEEEPLSKTISDVVTPLWQVPYEEQLKIKEEECCKVLKQLTKEIGVNNKPLAPWLFEQKQKYNRLCCPLERVIPSPIQTEYRNKSEFLIGYGVNQEDKTVGCRLSKYKGGTCAVVEPFDTINIPPVTKNVVRAFQEYIRSTPYDVYSPETYAGHWKQLTVRTSRKGQVMAIVYFNPQKLPKEDLSELKTSLSMYFTEGSGKESGVTSLYFVEEGQRKSPHLEDLPVEHIAGDQYIYEDLLGLTFRISPHAFFQVNTPAAEVLYSAIADWAQLDQNTTVLDVCCGTGTIGISLAKKVKKVVGIELCQEAIADAKANAQLNNLDNVEFRCGKAEDIFPTLIYSFTFPSPLAIVDPPRAGLHSKVIIAIRKAEHLKQLIYVSCNPKAAMNNFIDLCRAPSNRVKGRPFRPIKALAVDLFPQTPHFELVILFERTEPAEIVTDPSKSTEPSLVP

Target ORF information:

RefSeq Version	XM_012956249.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tRNA methyltransferase 2 homolog A (trmt2a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956249.3

ORF Insert Sequence:

ATGCTGATTT CTGATTTGAA GATGGAAACC TGCGCGAATG AAAACATCTG CATCTCTGAA 
GAAATAAGTC AAGAAGGTTC CCCAGCAGAG GAAGATGGAA GCAATGAAAT GGCAAAATCT 
TCTGCTGAGT CCTGTCCAGA CAGCAAGAAT AAAGCAGGGA TTTATAGATA CATCAAAGAC 
GATCTCTTCA CTTCAGAGAT TTTCAAAGTA GAAATACAAA ACTTGCCAAA ATACATATGC 
TTTAATGACT TAAAAAAATT CCTTGGCAAG TATGGGCTAA ATCCTCATAA GATTAAACTC 
ATGAATAAAC AAACTTTTGC ATTTGTGACT TTTAAAAGTG AAGAAGAGCG TGACAAAGCT 
ATGAAAGTCA TACATGGGGT GAAGTGGAAG AACCGTACAC TTAGTGTTCG ACTTGCAAAG 
CCCAGGGCTG ACCCCATAAT GCAAAAAAGA AAGCAGGAAG AAGGAGATGG TGAACAGCCA 
GAAAGCAAGC GTCAGGCTGC ACACAATGAT GCAGAGGAGG AGCCCCTCAG TAAAACGATC 
TCGGATGTAG TGACTCCTTT GTGGCAAGTT CCATATGAAG AACAGCTGAA AATTAAGGAG 
GAAGAATGTT GCAAAGTATT GAAGCAGTTA ACAAAGGAAA TAGGAGTGAA TAACAAGCCA 
CTAGCCCCTT GGCTGTTTGA GCAGAAACAG AAGTACAATA GACTCTGCTG TCCCCTGGAA 
CGTGTCATAC CATCTCCAAT ACAGACAGAG TATCGTAACA AATCTGAATT CCTTATTGGA 
TATGGTGTTA ACCAGGAAGA CAAAACAGTG GGCTGCAGGC TTAGCAAATA TAAAGGAGGC 
ACATGCGCTG TGGTTGAGCC ATTTGATACA ATAAACATTC CCCCTGTTAC CAAGAATGTT 
GTGAGAGCTT TCCAGGAATA CATAAGGAGC ACTCCATATG ATGTCTATAG CCCTGAAACA 
TATGCTGGTC ACTGGAAGCA GCTTACTGTC CGTACCAGCA GAAAGGGGCA GGTTATGGCA 
ATAGTGTATT TTAACCCCCA GAAACTGCCT AAGGAGGATC TCTCAGAGCT AAAAACGTCA 
CTATCAATGT ATTTCACTGA AGGGTCGGGG AAGGAAAGTG GTGTCACCTC CCTTTATTTT 
GTTGAAGAGG GTCAAAGGAA GTCTCCCCAC CTGGAGGATT TGCCTGTGGA ACATATAGCT 
GGAGACCAGT ATATCTATGA AGATCTACTT GGTCTAACCT TCAGAATTTC ACCTCATGCC 
TTCTTCCAGG TTAATACACC TGCAGCTGAG GTTCTGTATT CAGCCATTGC AGACTGGGCT 
CAGCTGGATC AGAACACCAC TGTTTTGGAT GTTTGCTGTG GGACTGGAAC CATTGGCATT 
TCTTTGGCTA AGAAAGTCAA AAAAGTTGTT GGAATTGAAC TGTGTCAGGA GGCTATTGCA 
GATGCCAAGG CAAATGCTCA ACTAAATAAT TTGGACAATG TAGAGTTCCG ATGTGGCAAA 
GCTGAGGACA TCTTTCCCAC CCTTATTTAC TCTTTCACGT TCCCAAGCCC ACTGGCCATT 
GTTGATCCAC CAAGAGCCGG CCTCCACTCC AAGGTGATTA TTGCAATTAG GAAAGCAGAG 
CATTTGAAGC AACTGATATA TGTTTCCTGC AATCCCAAGG CAGCAATGAA CAACTTTATT 
GACCTGTGCC GTGCTCCTTC CAATCGTGTC AAGGGGAGAC CATTTCGGCC AATCAAAGCC 
TTAGCTGTAG ACCTTTTCCC GCAGACTCCA CATTTTGAAT TGGTTATTCT GTTTGAGAGA 
ACGGAACCTG CAGAAATCGT AACTGATCCG TCTAAAAGCA CCGAGCCGTC ACTTGTGCCG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trmt2a ( XM_031895705.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751565.1 Xenopus tropicalis tRNA methyltransferase 2 homolog A (trmt2a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa47585
Clone ID Related Accession (Same CDS sequence)	XM_031895705.1
Accession Version	XM_031895705.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tRNA (uracil-5-)-methyltransferase homolog A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACCT GCGCGAATGA AAACATCTGC ATCTCTGAAG AAATAAGTCA AGAAGGTTCC 
CCAGCAGAGG AAGATGGAAG CAATGAAATG GCAAAATCTT CTGCTGAGTC CTGTCCAGAC 
AGCAAGAATA AAGCAGGGAT TTATAGATAC ATCAAAGACG ATCTCTTCAC TTCAGAGATT 
TTCAAAGTAG AAATACAAAA CTTGCCAAAA TACATATGCT TTAATGACTT AAAAAAATTC 
CTTGGCAAGT ATGGGCTAAA TCCTCATAAG ATTAAACTCA TGAATAAACA AACTTTTGCA 
TTTGTGACTT TTAAAAGTGA AGAAGAGCGT GACAAAGCTA TGAAAGTCAT ACATGGGGTG 
AAGTGGAAGA ACCGTACACT TAGTGTTCGA CTTGCAAAGC CCAGGGCTGA CCCCATAATG 
CAAAAAAGAA AGCAGGAAGA AGGAGATGGT GAACAGCCAG AAAGCAAGCG TCAGGCTGCA 
CACAATGATG CAGAGGAGGA GCCCCTCAGT AAAACGATCT CGGATGTAGT GACTCCTTTG 
TGGCAAGTTC CATATGAAGA ACAGCTGAAA ATTAAGGAGG AAGAATGTTG CAAAGTATTG 
AAGCAGTTAA CAAAGGAAAT AGGAGTGAAT AACAAGCCAC TAGCCCCTTG GCTGTTTGAG 
CAGAAACAGA AGTACAATAG ACTCTGCTGT CCCCTGGAAC GTGTCATACC ATCTCCAATA 
CAGACAGAGT ATCGTAACAA ATCTGAATTC CTTATTGGAT ATGGTGTTAA CCAGGAAGAC 
AAAACAGTGG GCTGCAGGCT TAGCAAATAT AAAGGAGGCA CATGCGCTGT GGTTGAGCCA 
TTTGATACAA TAAACATTCC CCCTGTTACC AAGAATGTTG TGAGAGCTTT CCAGGAATAC 
ATAAGGAGCA CTCCATATGA TGTCTATAGC CCTGAAACAT ATGCTGGTCA CTGGAAGCAG 
CTTACTGTCC GTACCAGCAG AAAGGGGCAG GTTATGGCAA TAGTGTATTT TAACCCCCAG 
AAACTGCCTA AGGAGGATCT CTCAGAGCTA AAAACGTCAC TATCAATGTA TTTCACTGAA 
GGGTCGGGGA AGGAAAGTGG TGTCACCTCC CTTTATTTTG TTGAAGAGGG TCAAAGGAAG 
TCTCCCCACC TGGAGGATTT GCCTGTGGAA CATATAGCTG GAGACCAGTA TATCTATGAA 
GATCTACTTG GTCTAACCTT CAGAATTTCA CCTCATGCCT TCTTCCAGGT TAATACACCT 
GCAGCTGAGG TTCTGTATTC AGCCATTGCA GACTGGGCTC AGCTGGATCA GAACACCACT 
GTTTTGGATG TTTGCTGTGG GACTGGAACC ATTGGCATTT CTTTGGCTAA GAAAGTCAAA 
AAAGTTGTTG GAATTGAACT GTGTCAGGAG GCTATTGCAG ATGCCAAGGC AAATGCTCAA 
CTAAATAATT TGGACAATGT AGAGTTCCGA TGTGGCAAAG CTGAGGACAT CTTTCCCACC 
CTTATTTACT CTTTCACGTT CCCAAGCCCA CTGGCCATTG TTGATCCACC AAGAGCCGGC 
CTCCACTCCA AGGTGATTAT TGCAATTAGG AAAGCAGAGC ATTTGAAGCA ACTGATATAT 
GTTTCCTGCA ATCCCAAGGC AGCAATGAAC AACTTTATTG ACCTGTGCCG TGCTCCTTCC 
AATCGTGTCA AGGGGAGACC ATTTCGGCCA ATCAAAGCCT TAGCTGTAGA CCTTTTCCCG 
CAGACTCCAC ATTTTGAATT GGTTATTCTG TTTGAGAGAA CGGAACCTGC AGAAATCGTA 
ACTGATCCGT CTAAAAGCAC CGAGCCGTCA CTTGTGCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751565.1
CDS	216..2057
Translation	METCANENICISEEISQEGSPAEEDGSNEMAKSSAESCPDSKNKAGIYRYIKDDLFTSEIFKVEIQNLPKYICFNDLKKFLGKYGLNPHKIKLMNKQTFAFVTFKSEEERDKAMKVIHGVKWKNRTLSVRLAKPRADPIMQKRKQEEGDGEQPESKRQAAHNDAEEEPLSKTISDVVTPLWQVPYEEQLKIKEEECCKVLKQLTKEIGVNNKPLAPWLFEQKQKYNRLCCPLERVIPSPIQTEYRNKSEFLIGYGVNQEDKTVGCRLSKYKGGTCAVVEPFDTINIPPVTKNVVRAFQEYIRSTPYDVYSPETYAGHWKQLTVRTSRKGQVMAIVYFNPQKLPKEDLSELKTSLSMYFTEGSGKESGVTSLYFVEEGQRKSPHLEDLPVEHIAGDQYIYEDLLGLTFRISPHAFFQVNTPAAEVLYSAIADWAQLDQNTTVLDVCCGTGTIGISLAKKVKKVVGIELCQEAIADAKANAQLNNLDNVEFRCGKAEDIFPTLIYSFTFPSPLAIVDPPRAGLHSKVIIAIRKAEHLKQLIYVSCNPKAAMNNFIDLCRAPSNRVKGRPFRPIKALAVDLFPQTPHFELVILFERTEPAEIVTDPSKSTEPSLVP

Target ORF information:

RefSeq Version	XM_031895705.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tRNA methyltransferase 2 homolog A (trmt2a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895705.1

ORF Insert Sequence:

ATGGAAACCT GCGCGAATGA AAACATCTGC ATCTCTGAAG AAATAAGTCA AGAAGGTTCC 
CCAGCAGAGG AAGATGGAAG CAATGAAATG GCAAAATCTT CTGCTGAGTC CTGTCCAGAC 
AGCAAGAATA AAGCAGGGAT TTATAGATAC ATCAAAGACG ATCTCTTCAC TTCAGAGATT 
TTCAAAGTAG AAATACAAAA CTTGCCAAAA TACATATGCT TTAATGACTT AAAAAAATTC 
CTTGGCAAGT ATGGGCTAAA TCCTCATAAG ATTAAACTCA TGAATAAACA AACTTTTGCA 
TTTGTGACTT TTAAAAGTGA AGAAGAGCGT GACAAAGCTA TGAAAGTCAT ACATGGGGTG 
AAGTGGAAGA ACCGTACACT TAGTGTTCGA CTTGCAAAGC CCAGGGCTGA CCCCATAATG 
CAAAAAAGAA AGCAGGAAGA AGGAGATGGT GAACAGCCAG AAAGCAAGCG TCAGGCTGCA 
CACAATGATG CAGAGGAGGA GCCCCTCAGT AAAACGATCT CGGATGTAGT GACTCCTTTG 
TGGCAAGTTC CATATGAAGA ACAGCTGAAA ATTAAGGAGG AAGAATGTTG CAAAGTATTG 
AAGCAGTTAA CAAAGGAAAT AGGAGTGAAT AACAAGCCAC TAGCCCCTTG GCTGTTTGAG 
CAGAAACAGA AGTACAATAG ACTCTGCTGT CCCCTGGAAC GTGTCATACC ATCTCCAATA 
CAGACAGAGT ATCGTAACAA ATCTGAATTC CTTATTGGAT ATGGTGTTAA CCAGGAAGAC 
AAAACAGTGG GCTGCAGGCT TAGCAAATAT AAAGGAGGCA CATGCGCTGT GGTTGAGCCA 
TTTGATACAA TAAACATTCC CCCTGTTACC AAGAATGTTG TGAGAGCTTT CCAGGAATAC 
ATAAGGAGCA CTCCATATGA TGTCTATAGC CCTGAAACAT ATGCTGGTCA CTGGAAGCAG 
CTTACTGTCC GTACCAGCAG AAAGGGGCAG GTTATGGCAA TAGTGTATTT TAACCCCCAG 
AAACTGCCTA AGGAGGATCT CTCAGAGCTA AAAACGTCAC TATCAATGTA TTTCACTGAA 
GGGTCGGGGA AGGAAAGTGG TGTCACCTCC CTTTATTTTG TTGAAGAGGG TCAAAGGAAG 
TCTCCCCACC TGGAGGATTT GCCTGTGGAA CATATAGCTG GAGACCAGTA TATCTATGAA 
GATCTACTTG GTCTAACCTT CAGAATTTCA CCTCATGCCT TCTTCCAGGT TAATACACCT 
GCAGCTGAGG TTCTGTATTC AGCCATTGCA GACTGGGCTC AGCTGGATCA GAACACCACT 
GTTTTGGATG TTTGCTGTGG GACTGGAACC ATTGGCATTT CTTTGGCTAA GAAAGTCAAA 
AAAGTTGTTG GAATTGAACT GTGTCAGGAG GCTATTGCAG ATGCCAAGGC AAATGCTCAA 
CTAAATAATT TGGACAATGT AGAGTTCCGA TGTGGCAAAG CTGAGGACAT CTTTCCCACC 
CTTATTTACT CTTTCACGTT CCCAAGCCCA CTGGCCATTG TTGATCCACC AAGAGCCGGC 
CTCCACTCCA AGGTGATTAT TGCAATTAGG AAAGCAGAGC ATTTGAAGCA ACTGATATAT 
GTTTCCTGCA ATCCCAAGGC AGCAATGAAC AACTTTATTG ACCTGTGCCG TGCTCCTTCC 
AATCGTGTCA AGGGGAGACC ATTTCGGCCA ATCAAAGCCT TAGCTGTAGA CCTTTTCCCG 
CAGACTCCAC ATTTTGAATT GGTTATTCTG TTTGAGAGAA CGGAACCTGC AGAAATCGTA 
ACTGATCCGT CTAAAAGCAC CGAGCCGTCA CTTGTGCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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trmt2a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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