hectd4 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following hectd4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hectd4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa03515 XM_004910504.3
Latest version!
Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 4 (hectd4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa31528 XM_012968793.2
Latest version!
Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 4 (hectd4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa31529 XM_012968794.2
Latest version!
Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 4 (hectd4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa31530 XM_012968799.2
Latest version!
Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 4 (hectd4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa31531 XM_012968806.2
Latest version!
Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 4 (hectd4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa49024 XM_012968793.3
Latest version!
Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 4 (hectd4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa49025 XM_012968794.3
Latest version!
Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 4 (hectd4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa49026 XM_012968799.3
Latest version!
Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 4 (hectd4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa49027 XM_004910504.4
Latest version!
Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 4 (hectd4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa03515
    Clone ID Related Accession (Same CDS sequence) XM_004910504.3
    Accession Version XM_004910504.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13212bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product probable E3 ubiquitin-protein ligase HECTD4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004910504.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    12781
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    13021
    13081
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    13201
    ATGGGGTCGG CGGCTGCCGG GGACTCGGCT CAGTGGCTGT CGGTGAAAGA AGAGACTATC 
    TTTTTACATG ATGGGCTGAT CCGGGTCACA GACCTGGCGG AGTTGCCCAG CGAAATCGGG
    GTCAGTGAGG CCGGGGACGC AGAACTGGAG ATATTGACTT TTGAGACTAA GAACCCTATT
    GAGCTAGCAG AGCATTTACG TGCAGTGTGT GGGAACCAGA GCAATGCCTA TGCACGATTG
    CTGGAATACC GCTTGAATGC GTTACGTGGG TTATGGAATG CCCAACGCCA GCTGGCGCTT
    GAGGATCAAC ATGAAAGAGA TGGGGCTGAT GACGAAACCT TGGCCCTGCT TAAACGGCAG
    GGTTTGTTGC AACAGCCGGA ACAGGCTCCA TTTACATCTC GAATGGGGCT TTTACTGGTG
    TTCCCCCTTA TCCAGTCCCA AAGTAAAACA GACCCTTCAC TTTGTAACTT GACAGCTGAG
    GTGCTTTTAA ATTGCCTTCG GGACTGCCAA CCCCTGAGCT TGACTAAAGA ACCAGCTGAC
    TGCCTGAATG GAATAGAGTC CTTACTGTGT TCTTGGTTAG AAGACACTCC AAATGTGGGC
    CAACAAGTCT CCTTTAAGCA AAAGGAAAAT TCTGCTGCTG CTTTGGTTGC CTTAGCTTGT
    GCTAGGGGAT CATTAAAAAC ATTTATTCAC ACTGTACACT TGCTGCAAAA ACAGACTGAC
    CTAGGATCCC TTCCTGTGGC AGATGTATTG TATAGGTTGC TGCTTTTAGA GGGAGGTCCA
    GGTTCTCCCT CTTGTTTACT GGGAAGCAAA CACATTGTAT CCTGGGGTTT TGAGGATATG
    CTTCCAGCTC CTGATGGGAT CAGTGGTTCA AACAATGAGA ACAAAGATGC AGATTTGGGT
    CGCTGTGTAG CATGCGATGG GCTTTATCTT TACACAACTA ACTCTGTAGG TAGAGGATTG
    AGCAAAGTAG GGTCTGGGCT GCATGGCACT TTACGAGGTT TTGTTTATTC CCGAAACGAG
    GAGCTAGAGG CTGGCTGGAT TGCTTATGGA AGTGGGAAGA TACTTCACAG GCCTGCCTCC
    TTTGATAACA AACCTCAATA TCTTTTCCAA TTAATTGACC AATACACACT TCAGGTCTCT
    TATATAGTCA GCATGCCAGC CAATCATTTC CCGGTTGGCA GCAATATGAC TACGGTGCAC
    CTCTCCTCGG ATGGCACGTA TTTTTACTGG ATTTGGTCTC CAGCTAGTTT AAATGAGAAA
    ACCCCCAAAG GACACTCTGT CTTCATGGAT ATATTTGAGT TAAATGTGCA AAATGGAGTA
    TGCATTGCAA GCCCTCTTCA TGAGCGCATT ATATTAATGA GGAAAGAGGG AGAATCTGCT
    AAAAGTATTA ATGAAATGCT TTTGAGCAGA TTATCACGAT ACAGAGCTTC TCCTTCAGCA
    ACTCTAGCAG CACTTACAGG ATCTACAATC TCTAACACTC TAAAAGAGGA CCAGACTGCT
    GCAAATACGA GCTGCGGGCT TCCTTTGAAA ATGTTAAGAA AGACTCCAAT GTATACTTGT
    GGCACATACT TAGTACTACT CGTGCCACCT CCGGGTGGGT CAGGAAGTTC AGCAACAAGA
    TCTTTGTTTG GTGGAACCAG CGGCCTCTCC TCTTTAAAAA TTCTGGCTTC CAGCTTGGTA
    TTTAACATTT CTGATGGGCA GTTCACCAAC AGAGCAGATC TTATTGATGC ATCTGGAAGT
    TCTCTTGGAA GGGGTGCTTT GGTGACAGGC CTAGGTGCCT GCTATGATAC AATGAATAAC
    ATGATCTGGA CGTGCTCCAA TGATTACATC GATCAGTGGT GTAATCCTGG AAACCAGGCT
    TTCCATGTGG TGTGTCAGAG GCTTGGTGTC ACTCACATCA TTGCTGATCC CAAAGGAGAG
    GCAGTATCAA CAAATGAAGT TATAAACCAG TTGCTTCACC ATGTGGGTGC TATGTGTATT
    CACCAACTCA ATCTCCTGGC TAATAGCAAC AGTTCTCCAA TCAGTACCTT TCTGGGCAAA
    CAACATCCTA TAGAGCCACA TCATCTTAAT AGTATATGTG ACATCATGGA GAAAGCCATG
    GTCAATGGTG ATAAATGCAT TATTCGCTGC ATCTTGGTTG TTTTCCAGGT GGTATTTAAA
    TTCTTTCTAA ACCCACAAAC TGAGAGGAAT CGAGAAACCA TAAGGAGGGC TGGTTTGCTC
    CTTTGGCAGT TGCTCATGGC GCCTAAGGAC CAAATTTGTC CTGAAATACA GAGAGAGGTC
    TGCTTGGCTA TTAGCTTTGG TTTAAACATT TTGAATCCAG GAGAGCTAGA AATTAATAAC
    TTGCTCAAGC TGATTTTAAC TGAAGGGGAC AGAAATTGTG GCCTCTCCCA ACTCCGTGAT
    GTCATTTTGA CAAACCTCAC TGAACAGCTT CAGAATAACA GGTTTGGAAG TGAAGATGAT
    GAACATTACA GGCTTAATGA TGAACTATTG CACTATATTT TGAAGATTGT TGTTCGTGAA
    TCCTGTGTTC TGATCACCAA GTGCCAAACA GTGTCTAAAG AAGAATTCCA AAGACTCTTG
    TCAACAGTGC CTGTGGCTTC TCCCTGTCTA TGCTATTTAA TGGCTGTACA GAATCATCTC
    CTGAGTAACA CTGTCTTAAT TAAACCTGAT GACAGCGATG ACAGTGACAA CTCCCTGCAG
    GGGGAGACAT TGAAGGAACT CCAGGCCAGC ATCTTGGCTT TGGCAACCCA GATACTTTCT
    GGTTGTGATG AAGTTCTGGA TATTCTGCAG CGAGTGACCA CTGCTCTTAT CAACAGCGAC
    CTGTTAGACA GAGATTTAAG ACTGAAAGGC CTTGAACAGA TCACAAAGTC CACCCTGCTT
    GGCCATCTCC TCCCAGTACT TCTCACATCC CTTATGCATC CAAACTTACA GACATTAACC
    ATGGCAGATG CGCTTATGCC TCAGCTGGTG CAGCTTGTTC TTTACACTAG CCAGACTGCA
    CTGCTGCTTA AAACCCAGTG TCCAGTATTG GCAGATACAG GGGACACCAC CAGTGGAATT
    CCAGAGCAAA AAAACAAACT GTTAACTGAT GACAGGATGC TGGAAGAAAA GGAGGAGCCT
    GGTTTCCTCA CTGGGCTAAA GATTCCTGCG CCATGGGCTG CAGGAAAGAC TGTGGAAACA
    TCTCATCCAG TCAGAGACAA TTACAAGTTT AAAGAGACTG TGCACATTCC TGGAGCTCGT
    TGTTTGTACC TCCGGTTTGA CAGCAGGTGC TCTTCACAGT ACGATTATGA TAAGCTGGTG
    ATATATGCGG GACCCAACAC AAACAGCAGA AAAGTTGCCG AATATGGTGG AAATACCCTG
    GGGTATGGCA GTAGAAGTGT TTTAGGTACA GGCTGGCCAA AAGATTTGGT TAAGGTGGAA
    GGGGATACTG TGACATTCTC CTTCGAAATG AGAAGTGGGC GTGAGCACAA CACTCCTGAT
    AAAGCAATGT GGGGTTTTGC GTGCACTGTC AGAGCTCAGG AGTCCTCAGA AGATGTGTCA
    GGGGGCTTGC CATTTCTAGT AGACCTGGCT TTAGGTCTGT CTGTCTTGGC TTGTTCCATG
    CTGCGTATAT TGTACAATGG TCCAGAAATC ACAAAAGAAG AGTTGACTTG TCACGATCTG
    TTGAGGTCAA AGCTTTTGCA GAGGTGTCAG TGGCAGGTGG AGGCTAATGG TGCTTTATCC
    CCGGCACTCA CCCCAAGCCC TTCGCCGCTG CCCCTTACTA TAGAAGAAGA CCAAGAATTT
    ACGTATCCAT CTGACATCTT GTTGCCACCA GTTGGGAACT ATTTTGAACT GCCAAGAATC
    AGACTGCCTC CTGGTATTAT GATCAAGCTC AGGGAAATAT CTGGACGCGC TCGACCACAG
    TTTAGACCAA GCATAAAAGA GGTTATCCAG CCTGATGTGA TGGAAGAAAT GGTTGTATCT
    TGCGTCATTA AACACTTGAA CCTGGTCGAT GCATTGCAGT CTTTAATAAA CTTCCAGTAT
    CGAGAAGAAC ATCTAGAAGA ATATGATTTA TTATGTAAAA TAATGGGGGA AACTTTTAAG
    AAGCTCAATG CAATGGAAAG ACAATTGCAG AGTGTTGCAG AACTGGAACA AAAGTGGCAA
    AGTGAAGTGG AAGATGCTCA GAATGGAAAG CTGGAGAACA ACGCTCCCTT TTTTTACGAC
    TATCATTTTA ATGAGAGCAA GTTAAAGGAA CTTGAACTTT TATGTTCTAT GAAAGAGGTC
    CCTTCTGATG GAAGTGATCC AGAAAACATG GTTTTCTCTC TGCGAGAGAA GTTTTTTCAA
    GAGGTGAACT CCCTGATCCA AAAACCTTCC CACCCAATGG CTAAGACCAA AACATTGGTT
    AAGAGTCTGA TGAATAGAGC AGAGCTCCTG TTACATGTGA CTATTGCAGC ACATTCTGGT
    ATCACGAGAA GCATATCTGG CACTCCAACT GAACCTCAAG CTGGCAAGTC TATCTCAGAA
    GCAAAAGTTA TATCTCAAGC CATGCGCCAG CCTGTTTTCC TCCGCAGCAT GTCTGCCCCT
    TCTGACCTGG AAATGATTGG AAGTAGTGAC TTAGAGTTCA GCAGGTCTAA TCCAAGGAGA
    AGGAACATCA CTAGCCACAG GAGCAGCTCC TTCACTCTGC TTCAGTCTTT AGCTATTGAA
    GACTGCAAGG ATAAACCAAC ATACAGTGTC CTTTTAGGGC AACTGTTTGC CTTCATTGGG
    ACCAATCCCG ACCAAGCAGT ATCCAGTAGC AGCTTTCTAC TTGCAGCCCA AACCAGATGG
    CGTCGTGGTA ATACTCGCAA ACAAGCCCTT GTACACATGA GAGAATTACT GACTGCTGCT
    GTCCGTGTTG GGGGTGTGAC ACATTTGGTT GGACCAGTAA CAATGGTGCT ACAGGGAGGA
    CCAAGAATTG AAGAGCTCAC CTGTGGAGGA ATGGTGGAGC TTGTTCAAGA GGCATTTGGA
    GAGACCATGA CCTCAGTGGT GTCACTTTGT GCACGGTATC CCATTGCCTG TGCAAATAGC
    ATTGGGCTTT TGTGTACTAT ACCTTACACA AGGAGTGAAG AAAAATGTTT AGTTCGCAGT
    GGGCTTGTTC AACTGATGGA CCGACTCTGC AGCTTAAGCA ACCAGAGAGA GTCCAGCTCA
    AATGAAAAGC AAACCAAGAA ACAGAAAGTA GCAACTATGG CATGGGCAGC CTTCCAAGTA
    CTGGCCAATA GATGTGTAGA ATGGGAGAAA GAAGAAGGTG GTTCTACTGA GGCGGTACAC
    TCTGGTTTGG CTAGACAAGT CTCCACCCTC CTCACTAACC ATCTAGCAAG GGCTACAGAG
    TGCTGTGGAA ACCAAGCTGC AGGGAATGAT GCCCTACAGG ACGTTCTGAG CCTTCTCAAT
    GATCTCTCTA GGAGTCAGAT AGGGAAGGCA ATTCTGAGCC AGCCTGCTTG TGTGTCCAAA
    CTTCTTTCGT TGCTGCTGGA CCAGCGTCCT TCTCCAAAGC TTGTGCTCAT AATTCTGCAG
    CTGTGCCGCG CTGCACTTCC TCTCATGAGT GTTGAAGACT GTGGAAATGT AGAACTTCCT
    CCATGGAGTT ACTCTTTACC AGCTCTGGAC AGTGAACAGG GGGAGGATCC AAGTGACCCA
    GCCTCCAAAA TAGCATCTTT GTTGCTAGCA AAGTTGGCTG ATTATGTTGT TCCAGGATGT
    CAGACAGTTC TTTCTCCTAC TGCTTCTGAA CCAGACAGCT CATTGTCCAA AAACAGCCCA
    AAAAATTCCC AGAAAGCAGA TAAGGATGCA GAAGAAGAGA GTGAAGCAGT AGATGGCAAG
    CTCTCCATTT TTATTCATAA GCGAGAAGAC CAGTCATCCC ATGAGGTTCT TCAGCCTTTG
    CTTAGTAGTT CAGAGGGAAG GCCTTTTCGT CTTGGTACTG GAGCGAATAT GGAAAAAGTA
    GTAAAGATGG ATCGTGACAT GACTAAGAGT GGATGTTGTG AAGTAATCAC AGAGGAGGCT
    GCAGCTGCTC TCCGCAAAGC CACTAAATGG GCCCAGTCGG GTTTAATTGT TAGCATTGGG
    CCACCTGTGG AGACAGTTAG CCCTGATTCA GCGAGTGGAC TTTCCACTGG TGACAAAAAG
    AAAACAGCCC AGACCTCGAT CTGTAGGGAA AGGAATTCTG AACTTGCCAG GACTGATCCT
    GTACGCCCTT TCATTAGTGG CCATGTAGCA AACAGCATGG CTGCAGAAGT AATTGCTCTG
    CTTCACAGCC TGCTGATGGC ACCAGAGTCA AATGCTGCCC AAATCTGGAC AACAACAGCA
    GAGAAGGTTC TTTCTCGTGC CCTGATGTAT ATCCCACAGC TTGGCAAATA TGCAGAAAGC
    ATCCTAGAGA ACGGCAGCGG TAATGGCAGG AAGCTAGCAA AGCTACAAGG CATTGCAAGG
    CAAGCAGTTG CTGCATTATG TGCGCTTGGT GGATTTAAAG AAACCATTAA AATAGGTTCT
    GAAGTCCAGG TGTTGGGTAA AGGAATATCT GGGAGCATTG GAGTGGTTGT TTCTATTAAT
    GAACAAGAAG GTATTGCAAC AGTGAAATTT CCAGACATAG ATTTTAGAAA ATCATGCAAA
    GCATCTGACA CACTGACAGT ACCATTATTT AGGCTCTGCG TTCCACGGTC AGAGGCATTG
    CCTCTCCATA AACTGTCCAT TACTGAGAAG GTAGTGCAGG CAGTCCAGTC CATGTTACTT
    CCCCAGGAGG GCTGCCTATC TATTCATACC TCGCTTCCTG CAACAGGAGA TGCCTCAACT
    CCAGTAATGG CAGTGGTTCG ACTTCTTGCA GAGATCAGGA CAAGAGCTTG CTTGGTGATG
    GCTCAGCTGT TGGAGGACAG CTCATTTTGT GAAGAATTCA TACAGCAATG TCCAGCTGCT
    GTAGAAGTTC TAAACCTAGT GGCCCAGGAA TGCAGTCCTG GGGAGCGTCT CTCAGTGGTG
    GAAGTTCAGT GTGAAAGACT ACGAATGCTT TATCGGGACT GTGCTCGCCC CCCGCCACCA
    CCTTTGCAGG CTGAGCGCAG ACAGCCCAAG GAAATCACCT GGTCTCCTTC CAGGGTATTC
    CCTCCTGTGA GAGCCTGTAT GTTTTCATCC CATCTAACAG CAGTAACATT TCTGGCTGAC
    CCAAGTGCTG GTGGGGGACT TCCTCGCGGC ACATTTATAT ATGCAACATC CCCTGTTCCA
    GTACAGGCAC CATCTTTTTA CTGGGAAATA GAAGTAGTTT CCTATGGAGA CACAGATGAT
    GACACTGGCC CTATTGTATC ATTTGGGTTT GCTACAGAAG CAGAGAAGCG GGATGGAGCA
    TGGACAAACC CAGTCGGTAC CTGTCTTTTT CATAACAATG GTCGAGCTGT ACATTACAAT
    GGATCAAGTC TCTTACAGTG GAAGAGTGTT CGATTAGATG TGACTCTTGC TCCTGGTGAT
    GTTGCTGGAA TAGGTTGGGA GAGAACTGAA GGAACACCAC CACCCCCAGG GCAGCCTGCC
    AAGGGCAGAG TCTACTTTAC TTACTGTGGA CAGAGGCTTA CTCCGTTCTT AGAGGATGTC
    TCTGGGGGCA TGTGGCCAGT TGTGCATATT CAGAAAAAGA ACACTAAAGT CCGTGCAAAT
    TTTGGATGCA GACAGTTTGC TTTTGCCGAG GGTCAAGCTC ATCGCAATGC AGCAGATTTG
    TGCATTGATT TGGCTGAAGA GATAAGTGCA AACTTTGAAG CATTGCCCTT TGCTATGGCA
    TCTGACAGTG ATAATGATGC TGGGACAAGC ATAGCATCTG ATCCTGGAAC TCATGGACCT
    CCTTGCCGTA TTGCTGCAGT TTCTACTGTG CAACAGCAAT ATGACAGTGA TGCCTCATGC
    CGTTACAAGG TAGAGCTGAG TTATCAAGAC TTTGTGACCT CTGGACCTGA TCCACACCCA
    CCTCCCATTG CAGATGATGA AAGTGATGAT GATGACGATG ATGATATTCC TCGAGAGGAT
    CATTATGCTC TTTTGGTAAA AGCATGGGAG ACCAAAGTTT TTCCAACCAT TCGAAGACGT
    TTCCGTAACG AGGCAGAGAG AAAGTCTGGC CTGGACCAGA TTAAGGGAGC TCTTCAGCTA
    GGAATGGTGG ATATAGCGAG GCAAACTGTA GAGTTCCTCT ATGAAGAAAA TGGTGGCATT
    CCAAGAGATC TCTATCTTCC TACTATTGAG GATATCAAGG ATGAGGCAAA TAAATTCACC
    ATTGATAAAG TTCGCAAAGG ACTCACAGTG GTCATTCGAT CCCCAGATAG CAACAATGTA
    GCTGGGACAA CTGTGGGAAC AGCTTTACCC AAGTTTGCCA TCCGTGGTAT GCTGAAGACA
    TTCTCTCTAC ATGGGGTGGT TCTGGATGTG GATTCAGTTA ATGAGCTGGT TCAGGTAGAG
    ACTTACCTCC GGAGTGAAGG GGTATTAGTG AGATACTGGT ATCCCATAGA TATGCTAGAA
    AGACCTCCTG CGGGATACAG GAGAATGGCA ACCAATGGAC TGGTGACTTT AGATAGCACT
    AACATCCAGA TTCATAGAGA GCTGTTGCGG TGTGAGTCTG CACTTGCCAG GCTTTACTGT
    CGTGTGGCTT TGCTTAACAT CTTTGCTCCT AAGTCTCCGC ACACTTTCAC TCGCCTGTTC
    CATATTCCTG CTATCCGAGA CATTACATTG GAGCATTTAC AGCTTCTGTC AAATCAGCTT
    CTAATGCCAC CCCTTCCAGA CGGAACCATT ACCTCCAGCT CTATCCTTCT TGCCCAGTCC
    TTGCAACATT GCATCTACTC CCAGAACTGC TCAACAACAG ATTTGTTCTA CCAGGGCAAT
    GCACAGCCAA TAAGAGAGTG GCTTGGTGTG GCCATAACCA GGGCCCTGCA TCAAGGAGAG
    GACAGTCTCT TGGATCTGAC AAAACAAATC TGTTCCTTCT TGCAAAATGC GCCGGAACAG
    TTTCCATCTG AAGAATTTCC CATCTCTGAA TCCAAGCTCA GTATGGATGT TAATTTTCCT
    GGGGCAGCTT TTGTTATTGT TTCTTGTATA GAGAGTCCGT CTGGATTTAG AAAAGACTCC
    TCCTTGTACA AGGCTCCCTG GTCTAGAGTC ATGGTATATG GCCTTGGACA TAAAGTAAAG
    AGGAATGGAC AGCTGAACCT GATAGAATCA GTTTGTTATC CACGTGATGC CTCCCCAAGT
    AACACAGGCC TCACTCCCCC ACCAACCACA AACCAGTACC CAACAGCGAT TATTCCCACT
    GACAAGGTCC ACGTCAAACT TGGTGTATCA CCTACTCCTG GTGCTGTGCT GGTCATCCAT
    TCCCTGCCTC TAGAGTTTCC GTTGGCAATG GCATTCACAG AACAGCTGTT AACCTGGAAG
    TTGGAGGACA CAGAAGAGAA GTCAGAAGAT GAGCTTGATA CTGTTCCATC CTCTGCTCTG
    TTGCAAGTGG TAGAACTTTT AGGTACTTTG ATTTGGACAA CAGACCTTGC TGCAGTAATT
    AAGGAGCTTC TGTTCCATCT GTTGGCTGAA CTATTAAGGA AAGTTCACCA TCTAGAGCAG
    AAGAAGAACC CAAGTGGATT ATCTTCCTCG ATAGCCCTGC AGTTGAATCC ATGTCTAGCA
    ATGCTTATGG CTTTACAGAC AGAACTGCGC AAGCTGTATG ATGAAGAAAC TCAAAACTGG
    GCATCTGGGA ATGCATGTGG GGGGTCTGTA CCTGGAGCCT CTGAGCAGGG AAGATTTTCA
    ACTTACTTTC ATGCCCTCAT GGAAGTGTGC CTGGCAGTGG CAGAAGTGAC GCTCCCAATC
    AACATGAGCT CAGCAATCAG TGTGATGGCA TCTACAAATG CCACTAATCT GAGTGACTCT
    TCTTCTTCCT CTTCCTCTTC CCCTGGACAG ACTCCTCAAA GTCCAAGTCT GTTATCAAAG
    AGAAAGAAAG TTAAGATCAA ACGTGAGAAG ACTGCATCTG GAAAACGTAC ACCTTCACGT
    GGTGCAGAGA GTGATGCTGC TTTGCTTAGC ATTGGAGGAA GCAAACCTGA GGACATGTTG
    TGGTTCCATA GGGCATTGAC TTTATTGATG ATCCTAAGGC ATCTAACTAA CAAGGATCCA
    CAGGGGATGG GTGTCACAAA CGATGCTATT GCTGATGCTA GCCAGGCTTT AGTTGGTCCC
    ACAGCTCACA GTCGTCTCCT TGTGATTTCT GGCATCCCTA CACACCTAGA AGAATCAGTG
    GTCAGAAGTG CAATTAGAAA GGCATGTAAT GCTCATGGTG GAGTCTTTAA AGATGAAATC
    TACATTCCAT TGCAGGAAAC TGAAACCCCT AAACCCAAAG ACAGAATAGA AGGGGCAGAA
    TGCAAGACAG AAATAGAAAA ACCCTCTACG CAGCATACAA CCGACAATAT GGATATCAGC
    AGCTCGAGCA GTGTAACTCC TGCAGCAAGT GTCACGGCAT CAGCTTCTAC AAGCCAAGCT
    TCATTGTGCA GCTCCCAAGG GGGGGTATCA CGGACCACAA GTGAAACATC TATGGAGCAG
    TTTGCCCCAG AACATGCTGT GCCATCAGGG CTTCTGGAAC CAACTATTAT CTCCAGCCAG
    GAGAGTCTGG ATCTTTCTAT GTGCAGCACA GGAAGCCTCG GCAGTCTGGG AAGTCTTGTT
    GAACAGCTTG ATAATGTTGA AAACTCTTTG TCTGACGTGG GCTCCCTATA CACAGCTACA
    TCACTAGATA GCCATATGGC AGAGTCGCGG CCAATCAAAG GATTTGCAGT TGTAGAGGTG
    CGATCACGAG CTAAAATAGA GAAAATCCGT GCCACTTTAT TTAACAGTAG TGACCTCATT
    GGGTTATCTG GTTTGGATGG TGAAGATGAG CTGATGGAAA TGTCTACTGA GGAGATCTTG
    ACTGTTTCAA CAGTGAATCA GAGCTTGTTT GATACCCAAG GAATACCCGG TCTGGCTGAT
    TATTTCATGG ATAAATCAAT GAAAGGTGAT AAATTAGTCC CGGAAGCTCG TGATGTTCTG
    ACTGAGATTT TCAAGAGCTG CATTCACGCC GAGCAGATGC TGAGTCTGAC TCCAGCCAAG
    TTTATCAAAG TGTCTGATAT CTACCTTAGC AAGGAGCAAA TCAATTCACA GACCCCAGGG
    AACTTACTGC ATACCTTTTT CACCAATGTT CGACCACCTA AAAAAGTCCT GGAAGATCAA
    CTAACGCAGA TCTTAAGAAA ATATGGAATT CCCAAGCCTA AATTTGACAA AAGCAAATAC
    AGCAAAACTG GGAAGGAGCA ACATCCTGTG AAAGTTGTAA GCACCAAAAG ACCTGTGGGC
    AAACCACCGA CCAAAGACAA GGCTGTATTC AACAGCGTCA GGACTGCTTT AAGTGAGAAA
    AAACCAACTG TGAAGCCAAA ATCTCCGGAG AAGGGCAGGC CAGATGAGAA GGACCCAGAC
    AAATCTCCAA CAAAGAAGCA AGAAATTCCC GAGGAGAGAT ATCTGACGTT GGATGGATTT
    CACCGCTTTG TAGTAGATCG GGCTAAGCAA GACATTCGAA GCATCTGGAG AGCTGTCTTG
    GCGTGTGGAT ATGATCTTCA TTTTGAAAGG TGTGCCTGCA TTGATACAAG GCATGCTCAG
    AAATCTAGCA GAAAATGGAC CTTGGAAATG GATGTTGCTC TTGTTCAGTA TATCAACCGC
    CTTTGCCGTC ACCTTGCCAT AACGCCAGCC CGACTACACC CTCATGAGGT TTATCTGGAT
    CTTGCAGATA CGGCTGACCC AAGAGTCTCC TGCCTGCTGA ATGTACCAAT AGAAAGCCTG
    CGCCTCCGAT TTGCTCTTCT TCAGTCCCTC AACACTACTT TAGAGACCTT TTTCCTGCCC
    TTAGTGGAGC TACGGCAAAC AGACGTATAC ACACACAGCA TCGCATCCCT ACTCCAGCTA
    GCTAAAGGGT TAATCTTTTA CGATACAAAA GTGAATGTGA TGAACCGGGT ATTGAACGCA
    ACAGTGCAGA GGACAGCAGA TCATGCAGCC CCTGAGATCA CGCTGGACCC ACTAGAAATT
    GTAGGAGGGG AAATTCGTAC CTCAGAAAAC TCCTACTTCT GCCAGGCAGC CCGACAGTTG
    GCATGCATCC CTTCCTCACA ACTGTGTGTC AAACTGGCAA GTGGAGGAGA CCCCACGTAT
    GCATTTAACA TCCGCTTTAC TGGAGAGGAG GTGCATGGTA CAAGTGGCTC ATTCCGTCAT
    TTCCTCTGGC AAGTGTGTAA AGAACTACAG AGCTCCTCGC TGTCTCTTCT ACTGCTCTGC
    CCCAGCTCTG CAGTTAATAA GAATAAGGGA AAATATCTTC TAACTCCCAG TCCCATAACA
    TATGCAGAAG AGCAGTTGCT CCATTTCTTT GGCCAACTAC TGGGAATAGC CATCAGAGCC
    GATGTTCCAC TCCCTCTGGA TCTACTCCCC TGCTTCTGGA AGATGCTTGT AGGAGAACCT
    TTGGACCCAG AAACTGATCT TTATGAAGCA GATATTCTCA CCCACAACTA CATCAAGAAG
    TTTGAAAATG TAAATGATGA GTCAGAGCTG GAAGCTCTTT GTGCTGAAAT TGCCTCTCAG
    CATCTTCCTA CCGAAAGTCC AGATTCTCCT CATAAACCAG CCTGCAAATT TACATACTTG
    ACAATGACTG GTGAAGAAGT GGAGCTGTGC CCTAAGGGAA GACACATTTC TGTAGTCTGG
    GAAAACAAAG ATATATATGC AATGGGAATC CGGAACCTTC GTATACGTGA GCTGAAGAAC
    CAGGAGTGTA TGAGCGCAGT AAGGGCTGGG CTGGGCTCCA TTATACCTCT GCAGCTCCTC
    ACCACTCTAA ATGCTCTAGA GATGGAACTA AGGACATGTG GCCTACCATA TATTAATTTG
    GAATTCTTAA AGGCTCACAC CATGTATCAA GTGGGTCTAA TGGAGACAGA TCAACACATT
    GAGTATTTTT GGTCTGCCTT AGAAATGTTC ACTCAGGAAG AACTTTGCAA ATTCATAAAA
    TTCGCCTGCA ATCAGGAACG CATTCCCTTT ACATGTCCAT GCAAAGATGG TGGGCCAGAC
    ACGGCTCATG TTCCACCTTA TCCTATGAAA ATAGCACCAC CCGATGGAGC AACAGGCTCC
    CCAGATTCCA GGTACATCCG AGTGGAGACA TGTATGTTCA TGATCAAGCT TCCGCAGTAC
    TCGTCTCTAG AGATGATGCT GGAGAAACTG AGATATGCCA TTCACTACAG GGAAGACCCA
    CTCAGCGGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004910561.1
    CDS323..13534
    Translation

    Target ORF information:

    RefSeq Version XM_004910504.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 4 (hectd4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004910504.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGGGGTCGG CGGCTGCCGG GGACTCGGCT CAGTGGCTGT CGGTGAAAGA AGAGACTATC 
    TTTTTACATG ATGGGCTGAT CCGGGTCACA GACCTGGCGG AGTTGCCCAG CGAAATCGGG
    GTCAGTGAGG CCGGGGACGC AGAACTGGAG ATATTGACTT TTGAGACTAA GAACCCTATT
    GAGCTAGCAG AGCATTTACG TGCAGTGTGT GGGAACCAGA GCAATGCCTA TGCACGATTG
    CTGGAATACC GCTTGAATGC GTTACGTGGG TTATGGAATG CCCAACGCCA GCTGGCGCTT
    GAGGATCAAC ATGAAAGAGA TGGGGCTGAT GACGAAACCT TGGCCCTGCT TAAACGGCAG
    GGTTTGTTGC AACAGCCGGA ACAGGCTCCA TTTACATCTC GAATGGGGCT TTTACTGGTG
    TTCCCCCTTA TCCAGTCCCA AAGTAAAACA GACCCTTCAC TTTGTAACTT GACAGCTGAG
    GTGCTTTTAA ATTGCCTTCG GGACTGCCAA CCCCTGAGCT TGACTAAAGA ACCAGCTGAC
    TGCCTGAATG GAATAGAGTC CTTACTGTGT TCTTGGTTAG AAGACACTCC AAATGTGGGC
    CAACAAGTCT CCTTTAAGCA AAAGGAAAAT TCTGCTGCTG CTTTGGTTGC CTTAGCTTGT
    GCTAGGGGAT CATTAAAAAC ATTTATTCAC ACTGTACACT TGCTGCAAAA ACAGACTGAC
    CTAGGATCCC TTCCTGTGGC AGATGTATTG TATAGGTTGC TGCTTTTAGA GGGAGGTCCA
    GGTTCTCCCT CTTGTTTACT GGGAAGCAAA CACATTGTAT CCTGGGGTTT TGAGGATATG
    CTTCCAGCTC CTGATGGGAT CAGTGGTTCA AACAATGAGA ACAAAGATGC AGATTTGGGT
    CGCTGTGTAG CATGCGATGG GCTTTATCTT TACACAACTA ACTCTGTAGG TAGAGGATTG
    AGCAAAGTAG GGTCTGGGCT GCATGGCACT TTACGAGGTT TTGTTTATTC CCGAAACGAG
    GAGCTAGAGG CTGGCTGGAT TGCTTATGGA AGTGGGAAGA TACTTCACAG GCCTGCCTCC
    TTTGATAACA AACCTCAATA TCTTTTCCAA TTAATTGACC AATACACACT TCAGGTCTCT
    TATATAGTCA GCATGCCAGC CAATCATTTC CCGGTTGGCA GCAATATGAC TACGGTGCAC
    CTCTCCTCGG ATGGCACGTA TTTTTACTGG ATTTGGTCTC CAGCTAGTTT AAATGAGAAA
    ACCCCCAAAG GACACTCTGT CTTCATGGAT ATATTTGAGT TAAATGTGCA AAATGGAGTA
    TGCATTGCAA GCCCTCTTCA TGAGCGCATT ATATTAATGA GGAAAGAGGG AGAATCTGCT
    AAAAGTATTA ATGAAATGCT TTTGAGCAGA TTATCACGAT ACAGAGCTTC TCCTTCAGCA
    ACTCTAGCAG CACTTACAGG ATCTACAATC TCTAACACTC TAAAAGAGGA CCAGACTGCT
    GCAAATACGA GCTGCGGGCT TCCTTTGAAA ATGTTAAGAA AGACTCCAAT GTATACTTGT
    GGCACATACT TAGTACTACT CGTGCCACCT CCGGGTGGGT CAGGAAGTTC AGCAACAAGA
    TCTTTGTTTG GTGGAACCAG CGGCCTCTCC TCTTTAAAAA TTCTGGCTTC CAGCTTGGTA
    TTTAACATTT CTGATGGGCA GTTCACCAAC AGAGCAGATC TTATTGATGC ATCTGGAAGT
    TCTCTTGGAA GGGGTGCTTT GGTGACAGGC CTAGGTGCCT GCTATGATAC AATGAATAAC
    ATGATCTGGA CGTGCTCCAA TGATTACATC GATCAGTGGT GTAATCCTGG AAACCAGGCT
    TTCCATGTGG TGTGTCAGAG GCTTGGTGTC ACTCACATCA TTGCTGATCC CAAAGGAGAG
    GCAGTATCAA CAAATGAAGT TATAAACCAG TTGCTTCACC ATGTGGGTGC TATGTGTATT
    CACCAACTCA ATCTCCTGGC TAATAGCAAC AGTTCTCCAA TCAGTACCTT TCTGGGCAAA
    CAACATCCTA TAGAGCCACA TCATCTTAAT AGTATATGTG ACATCATGGA GAAAGCCATG
    GTCAATGGTG ATAAATGCAT TATTCGCTGC ATCTTGGTTG TTTTCCAGGT GGTATTTAAA
    TTCTTTCTAA ACCCACAAAC TGAGAGGAAT CGAGAAACCA TAAGGAGGGC TGGTTTGCTC
    CTTTGGCAGT TGCTCATGGC GCCTAAGGAC CAAATTTGTC CTGAAATACA GAGAGAGGTC
    TGCTTGGCTA TTAGCTTTGG TTTAAACATT TTGAATCCAG GAGAGCTAGA AATTAATAAC
    TTGCTCAAGC TGATTTTAAC TGAAGGGGAC AGAAATTGTG GCCTCTCCCA ACTCCGTGAT
    GTCATTTTGA CAAACCTCAC TGAACAGCTT CAGAATAACA GGTTTGGAAG TGAAGATGAT
    GAACATTACA GGCTTAATGA TGAACTATTG CACTATATTT TGAAGATTGT TGTTCGTGAA
    TCCTGTGTTC TGATCACCAA GTGCCAAACA GTGTCTAAAG AAGAATTCCA AAGACTCTTG
    TCAACAGTGC CTGTGGCTTC TCCCTGTCTA TGCTATTTAA TGGCTGTACA GAATCATCTC
    CTGAGTAACA CTGTCTTAAT TAAACCTGAT GACAGCGATG ACAGTGACAA CTCCCTGCAG
    GGGGAGACAT TGAAGGAACT CCAGGCCAGC ATCTTGGCTT TGGCAACCCA GATACTTTCT
    GGTTGTGATG AAGTTCTGGA TATTCTGCAG CGAGTGACCA CTGCTCTTAT CAACAGCGAC
    CTGTTAGACA GAGATTTAAG ACTGAAAGGC CTTGAACAGA TCACAAAGTC CACCCTGCTT
    GGCCATCTCC TCCCAGTACT TCTCACATCC CTTATGCATC CAAACTTACA GACATTAACC
    ATGGCAGATG CGCTTATGCC TCAGCTGGTG CAGCTTGTTC TTTACACTAG CCAGACTGCA
    CTGCTGCTTA AAACCCAGTG TCCAGTATTG GCAGATACAG GGGACACCAC CAGTGGAATT
    CCAGAGCAAA AAAACAAACT GTTAACTGAT GACAGGATGC TGGAAGAAAA GGAGGAGCCT
    GGTTTCCTCA CTGGGCTAAA GATTCCTGCG CCATGGGCTG CAGGAAAGAC TGTGGAAACA
    TCTCATCCAG TCAGAGACAA TTACAAGTTT AAAGAGACTG TGCACATTCC TGGAGCTCGT
    TGTTTGTACC TCCGGTTTGA CAGCAGGTGC TCTTCACAGT ACGATTATGA TAAGCTGGTG
    ATATATGCGG GACCCAACAC AAACAGCAGA AAAGTTGCCG AATATGGTGG AAATACCCTG
    GGGTATGGCA GTAGAAGTGT TTTAGGTACA GGCTGGCCAA AAGATTTGGT TAAGGTGGAA
    GGGGATACTG TGACATTCTC CTTCGAAATG AGAAGTGGGC GTGAGCACAA CACTCCTGAT
    AAAGCAATGT GGGGTTTTGC GTGCACTGTC AGAGCTCAGG AGTCCTCAGA AGATGTGTCA
    GGGGGCTTGC CATTTCTAGT AGACCTGGCT TTAGGTCTGT CTGTCTTGGC TTGTTCCATG
    CTGCGTATAT TGTACAATGG TCCAGAAATC ACAAAAGAAG AGTTGACTTG TCACGATCTG
    TTGAGGTCAA AGCTTTTGCA GAGGTGTCAG TGGCAGGTGG AGGCTAATGG TGCTTTATCC
    CCGGCACTCA CCCCAAGCCC TTCGCCGCTG CCCCTTACTA TAGAAGAAGA CCAAGAATTT
    ACGTATCCAT CTGACATCTT GTTGCCACCA GTTGGGAACT ATTTTGAACT GCCAAGAATC
    AGACTGCCTC CTGGTATTAT GATCAAGCTC AGGGAAATAT CTGGACGCGC TCGACCACAG
    TTTAGACCAA GCATAAAAGA GGTTATCCAG CCTGATGTGA TGGAAGAAAT GGTTGTATCT
    TGCGTCATTA AACACTTGAA CCTGGTCGAT GCATTGCAGT CTTTAATAAA CTTCCAGTAT
    CGAGAAGAAC ATCTAGAAGA ATATGATTTA TTATGTAAAA TAATGGGGGA AACTTTTAAG
    AAGCTCAATG CAATGGAAAG ACAATTGCAG AGTGTTGCAG AACTGGAACA AAAGTGGCAA
    AGTGAAGTGG AAGATGCTCA GAATGGAAAG CTGGAGAACA ACGCTCCCTT TTTTTACGAC
    TATCATTTTA ATGAGAGCAA GTTAAAGGAA CTTGAACTTT TATGTTCTAT GAAAGAGGTC
    CCTTCTGATG GAAGTGATCC AGAAAACATG GTTTTCTCTC TGCGAGAGAA GTTTTTTCAA
    GAGGTGAACT CCCTGATCCA AAAACCTTCC CACCCAATGG CTAAGACCAA AACATTGGTT
    AAGAGTCTGA TGAATAGAGC AGAGCTCCTG TTACATGTGA CTATTGCAGC ACATTCTGGT
    ATCACGAGAA GCATATCTGG CACTCCAACT GAACCTCAAG CTGGCAAGTC TATCTCAGAA
    GCAAAAGTTA TATCTCAAGC CATGCGCCAG CCTGTTTTCC TCCGCAGCAT GTCTGCCCCT
    TCTGACCTGG AAATGATTGG AAGTAGTGAC TTAGAGTTCA GCAGGTCTAA TCCAAGGAGA
    AGGAACATCA CTAGCCACAG GAGCAGCTCC TTCACTCTGC TTCAGTCTTT AGCTATTGAA
    GACTGCAAGG ATAAACCAAC ATACAGTGTC CTTTTAGGGC AACTGTTTGC CTTCATTGGG
    ACCAATCCCG ACCAAGCAGT ATCCAGTAGC AGCTTTCTAC TTGCAGCCCA AACCAGATGG
    CGTCGTGGTA ATACTCGCAA ACAAGCCCTT GTACACATGA GAGAATTACT GACTGCTGCT
    GTCCGTGTTG GGGGTGTGAC ACATTTGGTT GGACCAGTAA CAATGGTGCT ACAGGGAGGA
    CCAAGAATTG AAGAGCTCAC CTGTGGAGGA ATGGTGGAGC TTGTTCAAGA GGCATTTGGA
    GAGACCATGA CCTCAGTGGT GTCACTTTGT GCACGGTATC CCATTGCCTG TGCAAATAGC
    ATTGGGCTTT TGTGTACTAT ACCTTACACA AGGAGTGAAG AAAAATGTTT AGTTCGCAGT
    GGGCTTGTTC AACTGATGGA CCGACTCTGC AGCTTAAGCA ACCAGAGAGA GTCCAGCTCA
    AATGAAAAGC AAACCAAGAA ACAGAAAGTA GCAACTATGG CATGGGCAGC CTTCCAAGTA
    CTGGCCAATA GATGTGTAGA ATGGGAGAAA GAAGAAGGTG GTTCTACTGA GGCGGTACAC
    TCTGGTTTGG CTAGACAAGT CTCCACCCTC CTCACTAACC ATCTAGCAAG GGCTACAGAG
    TGCTGTGGAA ACCAAGCTGC AGGGAATGAT GCCCTACAGG ACGTTCTGAG CCTTCTCAAT
    GATCTCTCTA GGAGTCAGAT AGGGAAGGCA ATTCTGAGCC AGCCTGCTTG TGTGTCCAAA
    CTTCTTTCGT TGCTGCTGGA CCAGCGTCCT TCTCCAAAGC TTGTGCTCAT AATTCTGCAG
    CTGTGCCGCG CTGCACTTCC TCTCATGAGT GTTGAAGACT GTGGAAATGT AGAACTTCCT
    CCATGGAGTT ACTCTTTACC AGCTCTGGAC AGTGAACAGG GGGAGGATCC AAGTGACCCA
    GCCTCCAAAA TAGCATCTTT GTTGCTAGCA AAGTTGGCTG ATTATGTTGT TCCAGGATGT
    CAGACAGTTC TTTCTCCTAC TGCTTCTGAA CCAGACAGCT CATTGTCCAA AAACAGCCCA
    AAAAATTCCC AGAAAGCAGA TAAGGATGCA GAAGAAGAGA GTGAAGCAGT AGATGGCAAG
    CTCTCCATTT TTATTCATAA GCGAGAAGAC CAGTCATCCC ATGAGGTTCT TCAGCCTTTG
    CTTAGTAGTT CAGAGGGAAG GCCTTTTCGT CTTGGTACTG GAGCGAATAT GGAAAAAGTA
    GTAAAGATGG ATCGTGACAT GACTAAGAGT GGATGTTGTG AAGTAATCAC AGAGGAGGCT
    GCAGCTGCTC TCCGCAAAGC CACTAAATGG GCCCAGTCGG GTTTAATTGT TAGCATTGGG
    CCACCTGTGG AGACAGTTAG CCCTGATTCA GCGAGTGGAC TTTCCACTGG TGACAAAAAG
    AAAACAGCCC AGACCTCGAT CTGTAGGGAA AGGAATTCTG AACTTGCCAG GACTGATCCT
    GTACGCCCTT TCATTAGTGG CCATGTAGCA AACAGCATGG CTGCAGAAGT AATTGCTCTG
    CTTCACAGCC TGCTGATGGC ACCAGAGTCA AATGCTGCCC AAATCTGGAC AACAACAGCA
    GAGAAGGTTC TTTCTCGTGC CCTGATGTAT ATCCCACAGC TTGGCAAATA TGCAGAAAGC
    ATCCTAGAGA ACGGCAGCGG TAATGGCAGG AAGCTAGCAA AGCTACAAGG CATTGCAAGG
    CAAGCAGTTG CTGCATTATG TGCGCTTGGT GGATTTAAAG AAACCATTAA AATAGGTTCT
    GAAGTCCAGG TGTTGGGTAA AGGAATATCT GGGAGCATTG GAGTGGTTGT TTCTATTAAT
    GAACAAGAAG GTATTGCAAC AGTGAAATTT CCAGACATAG ATTTTAGAAA ATCATGCAAA
    GCATCTGACA CACTGACAGT ACCATTATTT AGGCTCTGCG TTCCACGGTC AGAGGCATTG
    CCTCTCCATA AACTGTCCAT TACTGAGAAG GTAGTGCAGG CAGTCCAGTC CATGTTACTT
    CCCCAGGAGG GCTGCCTATC TATTCATACC TCGCTTCCTG CAACAGGAGA TGCCTCAACT
    CCAGTAATGG CAGTGGTTCG ACTTCTTGCA GAGATCAGGA CAAGAGCTTG CTTGGTGATG
    GCTCAGCTGT TGGAGGACAG CTCATTTTGT GAAGAATTCA TACAGCAATG TCCAGCTGCT
    GTAGAAGTTC TAAACCTAGT GGCCCAGGAA TGCAGTCCTG GGGAGCGTCT CTCAGTGGTG
    GAAGTTCAGT GTGAAAGACT ACGAATGCTT TATCGGGACT GTGCTCGCCC CCCGCCACCA
    CCTTTGCAGG CTGAGCGCAG ACAGCCCAAG GAAATCACCT GGTCTCCTTC CAGGGTATTC
    CCTCCTGTGA GAGCCTGTAT GTTTTCATCC CATCTAACAG CAGTAACATT TCTGGCTGAC
    CCAAGTGCTG GTGGGGGACT TCCTCGCGGC ACATTTATAT ATGCAACATC CCCTGTTCCA
    GTACAGGCAC CATCTTTTTA CTGGGAAATA GAAGTAGTTT CCTATGGAGA CACAGATGAT
    GACACTGGCC CTATTGTATC ATTTGGGTTT GCTACAGAAG CAGAGAAGCG GGATGGAGCA
    TGGACAAACC CAGTCGGTAC CTGTCTTTTT CATAACAATG GTCGAGCTGT ACATTACAAT
    GGATCAAGTC TCTTACAGTG GAAGAGTGTT CGATTAGATG TGACTCTTGC TCCTGGTGAT
    GTTGCTGGAA TAGGTTGGGA GAGAACTGAA GGAACACCAC CACCCCCAGG GCAGCCTGCC
    AAGGGCAGAG TCTACTTTAC TTACTGTGGA CAGAGGCTTA CTCCGTTCTT AGAGGATGTC
    TCTGGGGGCA TGTGGCCAGT TGTGCATATT CAGAAAAAGA ACACTAAAGT CCGTGCAAAT
    TTTGGATGCA GACAGTTTGC TTTTGCCGAG GGTCAAGCTC ATCGCAATGC AGCAGATTTG
    TGCATTGATT TGGCTGAAGA GATAAGTGCA AACTTTGAAG CATTGCCCTT TGCTATGGCA
    TCTGACAGTG ATAATGATGC TGGGACAAGC ATAGCATCTG ATCCTGGAAC TCATGGACCT
    CCTTGCCGTA TTGCTGCAGT TTCTACTGTG CAACAGCAAT ATGACAGTGA TGCCTCATGC
    CGTTACAAGG TAGAGCTGAG TTATCAAGAC TTTGTGACCT CTGGACCTGA TCCACACCCA
    CCTCCCATTG CAGATGATGA AAGTGATGAT GATGACGATG ATGATATTCC TCGAGAGGAT
    CATTATGCTC TTTTGGTAAA AGCATGGGAG ACCAAAGTTT TTCCAACCAT TCGAAGACGT
    TTCCGTAACG AGGCAGAGAG AAAGTCTGGC CTGGACCAGA TTAAGGGAGC TCTTCAGCTA
    GGAATGGTGG ATATAGCGAG GCAAACTGTA GAGTTCCTCT ATGAAGAAAA TGGTGGCATT
    CCAAGAGATC TCTATCTTCC TACTATTGAG GATATCAAGG ATGAGGCAAA TAAATTCACC
    ATTGATAAAG TTCGCAAAGG ACTCACAGTG GTCATTCGAT CCCCAGATAG CAACAATGTA
    GCTGGGACAA CTGTGGGAAC AGCTTTACCC AAGTTTGCCA TCCGTGGTAT GCTGAAGACA
    TTCTCTCTAC ATGGGGTGGT TCTGGATGTG GATTCAGTTA ATGAGCTGGT TCAGGTAGAG
    ACTTACCTCC GGAGTGAAGG GGTATTAGTG AGATACTGGT ATCCCATAGA TATGCTAGAA
    AGACCTCCTG CGGGATACAG GAGAATGGCA ACCAATGGAC TGGTGACTTT AGATAGCACT
    AACATCCAGA TTCATAGAGA GCTGTTGCGG TGTGAGTCTG CACTTGCCAG GCTTTACTGT
    CGTGTGGCTT TGCTTAACAT CTTTGCTCCT AAGTCTCCGC ACACTTTCAC TCGCCTGTTC
    CATATTCCTG CTATCCGAGA CATTACATTG GAGCATTTAC AGCTTCTGTC AAATCAGCTT
    CTAATGCCAC CCCTTCCAGA CGGAACCATT ACCTCCAGCT CTATCCTTCT TGCCCAGTCC
    TTGCAACATT GCATCTACTC CCAGAACTGC TCAACAACAG ATTTGTTCTA CCAGGGCAAT
    GCACAGCCAA TAAGAGAGTG GCTTGGTGTG GCCATAACCA GGGCCCTGCA TCAAGGAGAG
    GACAGTCTCT TGGATCTGAC AAAACAAATC TGTTCCTTCT TGCAAAATGC GCCGGAACAG
    TTTCCATCTG AAGAATTTCC CATCTCTGAA TCCAAGCTCA GTATGGATGT TAATTTTCCT
    GGGGCAGCTT TTGTTATTGT TTCTTGTATA GAGAGTCCGT CTGGATTTAG AAAAGACTCC
    TCCTTGTACA AGGCTCCCTG GTCTAGAGTC ATGGTATATG GCCTTGGACA TAAAGTAAAG
    AGGAATGGAC AGCTGAACCT GATAGAATCA GTTTGTTATC CACGTGATGC CTCCCCAAGT
    AACACAGGCC TCACTCCCCC ACCAACCACA AACCAGTACC CAACAGCGAT TATTCCCACT
    GACAAGGTCC ACGTCAAACT TGGTGTATCA CCTACTCCTG GTGCTGTGCT GGTCATCCAT
    TCCCTGCCTC TAGAGTTTCC GTTGGCAATG GCATTCACAG AACAGCTGTT AACCTGGAAG
    TTGGAGGACA CAGAAGAGAA GTCAGAAGAT GAGCTTGATA CTGTTCCATC CTCTGCTCTG
    TTGCAAGTGG TAGAACTTTT AGGTACTTTG ATTTGGACAA CAGACCTTGC TGCAGTAATT
    AAGGAGCTTC TGTTCCATCT GTTGGCTGAA CTATTAAGGA AAGTTCACCA TCTAGAGCAG
    AAGAAGAACC CAAGTGGATT ATCTTCCTCG ATAGCCCTGC AGTTGAATCC ATGTCTAGCA
    ATGCTTATGG CTTTACAGAC AGAACTGCGC AAGCTGTATG ATGAAGAAAC TCAAAACTGG
    GCATCTGGGA ATGCATGTGG GGGGTCTGTA CCTGGAGCCT CTGAGCAGGG AAGATTTTCA
    ACTTACTTTC ATGCCCTCAT GGAAGTGTGC CTGGCAGTGG CAGAAGTGAC GCTCCCAATC
    AACATGAGCT CAGCAATCAG TGTGATGGCA TCTACAAATG CCACTAATCT GAGTGACTCT
    TCTTCTTCCT CTTCCTCTTC CCCTGGACAG ACTCCTCAAA GTCCAAGTCT GTTATCAAAG
    AGAAAGAAAG TTAAGATCAA ACGTGAGAAG ACTGCATCTG GAAAACGTAC ACCTTCACGT
    GGTGCAGAGA GTGATGCTGC TTTGCTTAGC ATTGGAGGAA GCAAACCTGA GGACATGTTG
    TGGTTCCATA GGGCATTGAC TTTATTGATG ATCCTAAGGC ATCTAACTAA CAAGGATCCA
    CAGGGGATGG GTGTCACAAA CGATGCTATT GCTGATGCTA GCCAGGCTTT AGTTGGTCCC
    ACAGCTCACA GTCGTCTCCT TGTGATTTCT GGCATCCCTA CACACCTAGA AGAATCAGTG
    GTCAGAAGTG CAATTAGAAA GGCATGTAAT GCTCATGGTG GAGTCTTTAA AGATGAAATC
    TACATTCCAT TGCAGGAAAC TGAAACCCCT AAACCCAAAG ACAGAATAGA AGGGGCAGAA
    TGCAAGACAG AAATAGAAAA ACCCTCTACG CAGCATACAA CCGACAATAT GGATATCAGC
    AGCTCGAGCA GTGTAACTCC TGCAGCAAGT GTCACGGCAT CAGCTTCTAC AAGCCAAGCT
    TCATTGTGCA GCTCCCAAGG GGGGGTATCA CGGACCACAA GTGAAACATC TATGGAGCAG
    TTTGCCCCAG AACATGCTGT GCCATCAGGG CTTCTGGAAC CAACTATTAT CTCCAGCCAG
    GAGAGTCTGG ATCTTTCTAT GTGCAGCACA GGAAGCCTCG GCAGTCTGGG AAGTCTTGTT
    GAACAGCTTG ATAATGTTGA AAACTCTTTG TCTGACGTGG GCTCCCTATA CACAGCTACA
    TCACTAGATA GCCATATGGC AGAGTCGCGG CCAATCAAAG GATTTGCAGT TGTAGAGGTG
    CGATCACGAG CTAAAATAGA GAAAATCCGT GCCACTTTAT TTAACAGTAG TGACCTCATT
    GGGTTATCTG GTTTGGATGG TGAAGATGAG CTGATGGAAA TGTCTACTGA GGAGATCTTG
    ACTGTTTCAA CAGTGAATCA GAGCTTGTTT GATACCCAAG GAATACCCGG TCTGGCTGAT
    TATTTCATGG ATAAATCAAT GAAAGGTGAT AAATTAGTCC CGGAAGCTCG TGATGTTCTG
    ACTGAGATTT TCAAGAGCTG CATTCACGCC GAGCAGATGC TGAGTCTGAC TCCAGCCAAG
    TTTATCAAAG TGTCTGATAT CTACCTTAGC AAGGAGCAAA TCAATTCACA GACCCCAGGG
    AACTTACTGC ATACCTTTTT CACCAATGTT CGACCACCTA AAAAAGTCCT GGAAGATCAA
    CTAACGCAGA TCTTAAGAAA ATATGGAATT CCCAAGCCTA AATTTGACAA AAGCAAATAC
    AGCAAAACTG GGAAGGAGCA ACATCCTGTG AAAGTTGTAA GCACCAAAAG ACCTGTGGGC
    AAACCACCGA CCAAAGACAA GGCTGTATTC AACAGCGTCA GGACTGCTTT AAGTGAGAAA
    AAACCAACTG TGAAGCCAAA ATCTCCGGAG AAGGGCAGGC CAGATGAGAA GGACCCAGAC
    AAATCTCCAA CAAAGAAGCA AGAAATTCCC GAGGAGAGAT ATCTGACGTT GGATGGATTT
    CACCGCTTTG TAGTAGATCG GGCTAAGCAA GACATTCGAA GCATCTGGAG AGCTGTCTTG
    GCGTGTGGAT ATGATCTTCA TTTTGAAAGG TGTGCCTGCA TTGATACAAG GCATGCTCAG
    AAATCTAGCA GAAAATGGAC CTTGGAAATG GATGTTGCTC TTGTTCAGTA TATCAACCGC
    CTTTGCCGTC ACCTTGCCAT AACGCCAGCC CGACTACACC CTCATGAGGT TTATCTGGAT
    CTTGCAGATA CGGCTGACCC AAGAGTCTCC TGCCTGCTGA ATGTACCAAT AGAAAGCCTG
    CGCCTCCGAT TTGCTCTTCT TCAGTCCCTC AACACTACTT TAGAGACCTT TTTCCTGCCC
    TTAGTGGAGC TACGGCAAAC AGACGTATAC ACACACAGCA TCGCATCCCT ACTCCAGCTA
    GCTAAAGGGT TAATCTTTTA CGATACAAAA GTGAATGTGA TGAACCGGGT ATTGAACGCA
    ACAGTGCAGA GGACAGCAGA TCATGCAGCC CCTGAGATCA CGCTGGACCC ACTAGAAATT
    GTAGGAGGGG AAATTCGTAC CTCAGAAAAC TCCTACTTCT GCCAGGCAGC CCGACAGTTG
    GCATGCATCC CTTCCTCACA ACTGTGTGTC AAACTGGCAA GTGGAGGAGA CCCCACGTAT
    GCATTTAACA TCCGCTTTAC TGGAGAGGAG GTGCATGGTA CAAGTGGCTC ATTCCGTCAT
    TTCCTCTGGC AAGTGTGTAA AGAACTACAG AGCTCCTCGC TGTCTCTTCT ACTGCTCTGC
    CCCAGCTCTG CAGTTAATAA GAATAAGGGA AAATATCTTC TAACTCCCAG TCCCATAACA
    TATGCAGAAG AGCAGTTGCT CCATTTCTTT GGCCAACTAC TGGGAATAGC CATCAGAGCC
    GATGTTCCAC TCCCTCTGGA TCTACTCCCC TGCTTCTGGA AGATGCTTGT AGGAGAACCT
    TTGGACCCAG AAACTGATCT TTATGAAGCA GATATTCTCA CCCACAACTA CATCAAGAAG
    TTTGAAAATG TAAATGATGA GTCAGAGCTG GAAGCTCTTT GTGCTGAAAT TGCCTCTCAG
    CATCTTCCTA CCGAAAGTCC AGATTCTCCT CATAAACCAG CCTGCAAATT TACATACTTG
    ACAATGACTG GTGAAGAAGT GGAGCTGTGC CCTAAGGGAA GACACATTTC TGTAGTCTGG
    GAAAACAAAG ATATATATGC AATGGGAATC CGGAACCTTC GTATACGTGA GCTGAAGAAC
    CAGGAGTGTA TGAGCGCAGT AAGGGCTGGG CTGGGCTCCA TTATACCTCT GCAGCTCCTC
    ACCACTCTAA ATGCTCTAGA GATGGAACTA AGGACATGTG GCCTACCATA TATTAATTTG
    GAATTCTTAA AGGCTCACAC CATGTATCAA GTGGGTCTAA TGGAGACAGA TCAACACATT
    GAGTATTTTT GGTCTGCCTT AGAAATGTTC ACTCAGGAAG AACTTTGCAA ATTCATAAAA
    TTCGCCTGCA ATCAGGAACG CATTCCCTTT ACATGTCCAT GCAAAGATGG TGGGCCAGAC
    ACGGCTCATG TTCCACCTTA TCCTATGAAA ATAGCACCAC CCGATGGAGC AACAGGCTCC
    CCAGATTCCA GGTACATCCG AGTGGAGACA TGTATGTTCA TGATCAAGCT TCCGCAGTAC
    TCGTCTCTAG AGATGATGCT GGAGAAACTG AGATATGCCA TTCACTACAG GGAAGACCCA
    CTCAGCGGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31528
    Clone ID Related Accession (Same CDS sequence) XM_012968793.2
    Accession Version XM_012968793.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13242bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product probable E3 ubiquitin-protein ligase HECTD4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968793.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
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    2521
    2581
    2641
    2701
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    9781
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    10261
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    10501
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    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGGGGTCGG CGGCTGCCGG GGACTCGGCT CAGTGGCTGT CGGTGAAAGA AGAGACTATC 
    TTTTTACATG ATGGGCTGAT CCGGGTCACA GACCTGGCGG AGTTGCCCAG CGAAATCGGG
    GTCAGTGAGG CCGGGGACGC AGAACTGGAG ATATTGACTT TTGAGACTAA GAACCCTATT
    GAGCTAGCAG AGCATTTACG TGCAGTGTGT GGGAACCAGA GCAATGCCTA TGCACGATTG
    CTGGAATACC GCTTGAATGC GTTACGTGGG TTATGGAATG CCCAACGCCA GCTGGCGCTT
    GAGGATCAAC ATGAAAGAGA TGGGGCTGAT GACGAAACCT TGGCCCTGCT TAAACGGCAG
    GGTTTGTTGC AACAGCCGGA ACAGGCTCCA TTTACATCTC GAATGGGGCT TTTACTGGTG
    TTCCCCCTTA TCCAGTCCCA AAGTAAAACA GACCCTTCAC TTTGTAACTT GACAGCTGAG
    GTGCTTTTAA ATTGCCTTCG GGACTGCCAA CCCCTGAGCT TGACTAAAGA ACCAGCTGAC
    TGCCTGAATG GAATAGAGTC CTTACTGTGT TCTTGGTTAG AAGACACTCC AAATGTGGGC
    CAACAAGTCT CCTTTAAGCA AAAGGAAAAT TCTGCTGCTG CTTTGGTTGC CTTAGCTTGT
    GCTAGGGGAT CATTAAAAAC ATTTATTCAC ACTGTACACT TGCTGCAAAA ACAGACTGAC
    CTAGGATCCC TTCCTGTGGC AGATGTATTG TATAGGTTGC TGCTTTTAGA GGGAGGTCCA
    GGTTCTCCCT CTTGTTTACT GGGAAGCAAA CACATTGTAT CCTGGGGTTT TGAGGATATG
    CTTCCAGCTC CTGATGGGAT CAGTGGTTCA AACAATGAGA ACAAAGATGC AGATTTGGGT
    CGCTGTGTAG CATGCGATGG GCTTTATCTT TACACAACTA ACTCTGTAGG TAGAGGATTG
    AGCAAAGTAG GGTCTGGGCT GCATGGCACT TTACGAGGTT TTGTTTATTC CCGAAACGAG
    GAGCTAGAGG CTGGCTGGAT TGCTTATGGA AGTGGGAAGA TACTTCACAG GCCTGCCTCC
    TTTGATAACA AACCTCAATA TCTTTTCCAA TTAATTGACC AATACACACT TCAGGTCTCT
    TATATAGTCA GCATGCCAGC CAATCATTTC CCGGTTGGCA GCAATATGAC TACGGTGCAC
    CTCTCCTCGG ATGGCACGTA TTTTTACTGG ATTTGGTCTC CAGCTAGTTT AAATGAGAAA
    ACCCCCAAAG GACACTCTGT CTTCATGGAT ATATTTGAGT TAAATGTGCA AAATGGAGTA
    TGCATTGCAA GCCCTCTTCA TGAGCGCATT ATATTAATGA GGAAAGAGGG AGAATCTGCT
    AAAAGTATTA ATGAAATGCT TTTGAGCAGA TTATCACGAT ACAGAGCTTC TCCTTCAGCA
    ACTCTAGCAG CACTTACAGG ATCTACAATC TCTAACACTC TAAAAGAGGA CCAGACTGCT
    GCAAATACGA GCTGCGGGCT TCCTTTGAAA ATGTTAAGAA AGACTCCAAT GTATACTTGT
    GGCACATACT TAGTACTACT CGTGCCACCT CCGGGTGGGT CAGGAAGTTC AGCAACAAGA
    TCTTTGTTTG GTGGAACCAG CGGCCTCTCC TCTTTAAAAA TTCTGGCTTC CAGCTTGGTA
    TTTAACATTT CTGATGGGCA GTTCACCAAC AGAGCAGATC TTATTGATGC ATCTGGAAGT
    TCTCTTGGAA GGGGTGCTTT GGTGACAGGC CTAGGTGCCT GCTATGATAC AATGAATAAC
    ATGATCTGGA CGTGCTCCAA TGATTACATC GATCAGTGGT GTAATCCTGG AAACCAGGCT
    TTCCATGTGG TGTGTCAGAG GCTTGGTGTC ACTCACATCA TTGCTGATCC CAAAGGAGAG
    GCAGTATCAA CAAATGAAGT TATAAACCAG TTGCTTCACC ATGTGGGTGC TATGTGTATT
    CACCAACTCA ATCTCCTGGC TAATAGCAAC AGTTCTCCAA TCAGTACCTT TCTGGGCAAA
    CAACATCCTA TAGAGCCACA TCATCTTAAT AGTATATGTG ACATCATGGA GAAAGCCATG
    GTCAATGGTG ATAAATGCAT TATTCGCTGC ATCTTGGTTG TTTTCCAGGT GGTATTTAAA
    TTCTTTCTAA ACCCACAAAC TGAGAGGAAT CGAGAAACCA TAAGGAGGGC TGGTTTGCTC
    CTTTGGCAGT TGCTCATGGC GCCTAAGGAC CAAATTTGTC CTGAAATACA GAGAGAGGTC
    TGCTTGGCTA TTAGCTTTGG TTTAAACATT TTGAATCCAG GAGAGCTAGA AATTAATAAC
    TTGCTCAAGC TGATTTTAAC TGAAGGGGAC AGAAATTGTG GCCTCTCCCA ACTCCGTGAT
    GTCATTTTGA CAAACCTCAC TGAACAGCTT CAGAATAACA GGTTTGGAAG TGAAGATGAT
    GAACATTACA GGCTTAATGA TGAACTATTG CACTATATTT TGAAGATTGT TGTTCGTGAA
    TCCTGTGTTC TGATCACCAA GTGCCAAACA GTGTCTAAAG AAGAATTCCA AAGACTCTTG
    TCAACAGTGC CTGTGGCTTC TCCCTGTCTA TGCTATTTAA TGGCTGTACA GAATCATCTC
    CTGAGTAACA CTGTCTTAAT TAAACCTGAT GACAGCGATG ACAGTGACAA CTCCCTGCAG
    GGGGAGACAT TGAAGGTACA GGAACTCCAG GCCAGCATCT TGGCTTTGGC AACCCAGATA
    CTTTCTGGTT GTGATGAAGT TCTGGATATT CTGCAGCGAG TGACCACTGC TCTTATCAAC
    AGCGACCTGT TAGACAGAGA TTTAAGACTG AAAGGCCTTG AACAGATCAC AAAGTCCACC
    CTGCTTGGCC ATCTCCTCCC AGTACTTCTC ACATCCCTTA TGCATCCAAA CTTACAGACA
    TTAACCATGG CAGATGCGCT TATGCCTCAG CTGGTGCAGC TTGTTCTTTA CACTAGCCAG
    ACTGCACTGC TGCTTAAAAC CCAGTGTCCA GTATTGGCAG ATACAGGGGA CACCACCAGT
    GGAATTCCAG AGCAAAAAAA CAAACTGTTA ACTGATGACA GGATGCTGGA AGAAAAGGAG
    GAGCCTGGTT TCCTCACTGG GCTAAAGATT CCTGCGCCAT GGGCTGCAGG AAAGACTGTG
    GAAACATCTC ATCCAGTCAG AGACAATTAC AAGTTTAAAG AGACTGTGCA CATTCCTGGA
    GCTCGTTGTT TGTACCTCCG GTTTGACAGC AGGTGCTCTT CACAGTACGA TTATGATAAG
    CTGGTGATAT ATGCGGGACC CAACACAAAC AGCAGAAAAG TTGCCGAATA TGGTGGAAAT
    ACCCTGGGGT ATGGCAGTAG AAGTGTTTTA GGTACAGGCT GGCCAAAAGA TTTGGTTAAG
    GTGGAAGGGG ATACTGTGAC ATTCTCCTTC GAAATGAGAA GTGGGCGTGA GCACAACACT
    CCTGATAAAG CAATGTGGGG TTTTGCGTGC ACTGTCAGAG CTCAGGAGTC CTCAGAAGAT
    GTGTCAGGGG GCTTGCCATT TCTAGTAGAC CTGGCTTTAG GTCTGTCTGT CTTGGCTTGT
    TCCATGCTGC GTATATTGTA CAATGGTCCA GAAATCACAA AAGAAGAGTT GACTTGTCAC
    GATCTGTTGA GGTCAAAGCT TTTGCAGAGG TGTCAGTGGC AGGTGGAGGC TAATGGTGCT
    TTATCCCCGG CACTCACCCC AAGCCCTTCG CCGCTGCCCC TTACTATAGA AGAAGACCAA
    GAATTTACGT ATCCATCTGA CATCTTGTTG CCACCAGTTG GGAACTATTT TGAACTGCCA
    AGAATCAGAC TGCCTCCTGG TATTATGATC AAGCTCAGGG AAATATCTGG ACGCGCTCGA
    CCACAGTTTA GACCAAGCAT AAAAGAGGTT ATCCAGCCTG ATGTGATGGA AGAAATGGTT
    GTATCTTGCG TCATTAAACA CTTGAACCTG GTCGATGCAT TGCAGTCTTT AATAAACTTC
    CAGTATCGAG AAGAACATCT AGAAGAATAT GATTTATTAT GTAAAATAAT GGGGGAAACT
    TTTAAGAAGC TCAATGCAAT GGAAAGACAA TTGCAGAGTG TTGCAGAACT GGAACAAAAG
    TGGCAAAGTG AAGTGGAAGA TGCTCAGAAT GGAAAGCTGG AGAACAACGC TCCCTTTTTT
    TACGACTATC ATTTTAATGA GAGCAAGTTA AAGGAACTTG AACTTTTATG TTCTATGAAA
    GAGGTCCCTT CTGATGGAAG TGATCCAGAA AACATGGTTT TCTCTCTGCG AGAGAAGTTT
    TTTCAAGAGG TGAACTCCCT GATCCAAAAA CCTTCCCACC CAATGGCTAA GACCAAAACA
    TTGGTTAAGA GTCTGATGAA TAGAGCAGAG CTCCTGTTAC ATGTGACTAT TGCAGCACAT
    TCTGGTATCA CGAGAAGCAT ATCTGGCACT CCAACTGAAC CTCAAGCTGG CAAGTCTATC
    TCAGAAGCAA AAGTTATATC TCAAGCCATG CGCCAGCCTG TTTTCCTCCG CAGCATGTCT
    GCCCCTTCTG ACCTGGAAAT GATTGGAAGT AGTGACTTAG AGTTCAGCAG GTCTAATCCA
    AGGAGAAGGA ACATCACTAG CCACAGGAGC AGCTCCTTCA CTCTGCTTCA GTCTTTAGCT
    ATTGAAGACT GCAAGGATAA ACCAACATAC AGTGTCCTTT TAGGGCAACT GTTTGCCTTC
    ATTGGGACCA ATCCCGACCA AGCAGTATCC AGTAGCAGCT TTCTACTTGC AGCCCAAACC
    AGATGGCGTC GTGGTAATAC TCGCAAACAA GCCCTTGTAC ACATGAGAGA ATTACTGACT
    GCTGCTGTCC GTGTTGGGGG TGTGACACAT TTGGTTGGAC CAGTAACAAT GGTGCTACAG
    GGAGGACCAA GAATTGAAGA GCTCACCTGT GGAGGAATGG TGGAGCTTGT TCAAGAGGCA
    TTTGGAGAGA CCATGACCTC AGTGGTGTCA CTTTGTGCAC GGTATCCCAT TGCCTGTGCA
    AATAGCATTG GGCTTTTGTG TACTATACCT TACACAAGGA GTGAAGAAAA ATGTTTAGTT
    CGCAGTGGGC TTGTTCAACT GATGGACCGA CTCTGCAGCT TAAGCAACCA GAGAGAGTCC
    AGCTCAAATG AAAAGCAAAC CAAGAAACAG AAAGTAGCAA CTATGGCATG GGCAGCCTTC
    CAAGTACTGG CCAATAGATG TGTAGAATGG GAGAAAGAAG AAGGTGGTTC TACTGAGGCG
    GTACACTCTG GTTTGGCTAG ACAAGTCTCC ACCCTCCTCA CTAACCATCT AGCAAGGGCT
    ACAGAGTGCT GTGGAAACCA AGCTGCAGGG AATGATGCCC TACAGGACGT TCTGAGCCTT
    CTCAATGATC TCTCTAGGAG TCAGATAGGG AAGGCAATTC TGAGCCAGCC TGCTTGTGTG
    TCCAAACTTC TTTCGTTGCT GCTGGACCAG CGTCCTTCTC CAAAGCTTGT GCTCATAATT
    CTGCAGCTGT GCCGCGCTGC ACTTCCTCTC ATGAGTGTTG AAGACTGTGG AAATGTAGAA
    CTTCCTCCAT GGAGTTACTC TTTACCAGCT CTGGACAGTG AACAGGGGGA GGATCCAAGT
    GACCCAGCCT CCAAAATAGC ATCTTTGTTG CTAGCAAAGT TGGCTGATTA TGTTGTTCCA
    GGATGTCAGA CAGTTCTTTC TCCTACTGCT TCTGAACCAG ACAGCTCATT GTCCAAAAAC
    AGCCCAAAAA ATTCCCAGAA AGCAGATAAG GATGCAGAAG AAGAGAGTGA AGCAGTAGAT
    GGCAAGCTCT CCATTTTTAT TCATAAGCGA GAAGACCAGT CATCCCATGA GGTTCTTCAG
    CCTTTGCTTA GTAGTTCAGA GGGAAGGCCT TTTCGTCTTG GTACTGGAGC GAATATGGAA
    AAAGTAGTAA AGATGGATCG TGACATGACT AAGAGTGGAT GTTGTGAAGT AATCACAGAG
    GAGGCTGCAG CTGCTCTCCG CAAAGCCACT AAATGGGCCC AGTCGGGTTT AATTGTTAGC
    ATTGGGCCAC CTGTGGAGAC AGTTAGCCCT GATTCAGCGA GTGGACTTTC CACTGGTGAC
    AAAAAGAAAA CAGCCCAGAC CTCGATCTGT AGGGAAAGGA ATTCTGAACT TGCCAGGACT
    GATCCTGTAC GCCCTTTCAT TAGTGGCCAT GTAGCAAACA GCATGGCTGC AGAAGTAATT
    GCTCTGCTTC ACAGCCTGCT GATGGCACCA GAGTCAAATG CTGCCCAAAT CTGGACAACA
    ACAGCAGAGA AGGTTCTTTC TCGTGCCCTG ATGTATATCC CACAGCTTGG CAAATATGCA
    GAAAGCATCC TAGAGAACGG CAGCGGTAAT GGCAGGAAGC TAGCAAAGCT ACAAGGCATT
    GCAAGGCAAG CAGTTGCTGC ATTATGTGCG CTTGGTGGAT TTAAAGAAAC CATTAAAATA
    GGTTCTGAAG TCCAGGTGTT GGGTAAAGGA ATATCTGGGA GCATTGGAGT GGTTGTTTCT
    ATTAATGAAC AAGAAGGTAT TGCAACAGTG AAATTTCCAG ACATAGATTT TAGAAAATCA
    TGCAAAGCAT CTGACACACT GACAGTACCA TTATTTAGGC TCTGCGTTCC ACGGTCAGAG
    GCATTGCCTC TCCATAAACT GTCCATTACT GAGAAGGTAG TGCAGGCAGT CCAGTCCATG
    TTACTTCCCC AGGAGGGCTG CCTATCTATT CATACCTCGC TTCCTGCAAC AGGAGATGCC
    TCAACTCCAG TAATGGCAGT GGTTCGACTT CTTGCAGAGA TCAGGACAAG AGCTTGCTTG
    GTGATGGCTC AGCTGTTGGA GGACAGCTCA TTTTGTGAAG AATTCATACA GCAATGTCCA
    GCTGCTGTAG AAGTTCTAAA CCTAGTGGCC CAGGAATGCA GTCCTGGGGA GCGTCTCTCA
    GTGGTGGAAG TTCAGTGTGA AAGACTACGA ATGCTTTATC GGGACTGTGC TCGCCCCCCG
    CCACCACCTT TGCAGGCTGA GCGCAGACAG CCCAAGGAAA TCACCTGGTC TCCTTCCAGG
    GTATTCCCTC CTGTGAGAGC CTGTATGTTT TCATCCCATC TAACAGCAGT AACATTTCTG
    GCTGACCCAA GTGCTGGTGG GGGACTTCCT CGCGGCACAT TTATATATGC AACATCCCCT
    GTTCCAGTAC AGGCACCATC TTTTTACTGG GAAATAGAAG TAGTTTCCTA TGGAGACACA
    GATGATGACA CTGGCCCTAT TGTATCATTT GGGTTTGCTA CAGAAGCAGA GAAGCGGGAT
    GGAGCATGGA CAAACCCAGT CGGTACCTGT CTTTTTCATA ACAATGGTCG AGCTGTACAT
    TACAATGGAT CAAGTCTCTT ACAGTGGAAG AGTGTTCGAT TAGATGTGAC TCTTGCTCCT
    GGTGATGTTG CTGGAATAGG TTGGGAGAGA ACTGAAGGAA CACCACCACC CCCAGGGCAG
    CCTGCCAAGG GCAGAGTCTA CTTTACTTAC TGTGGACAGA GGCTTACTCC GTTCTTAGAG
    GATGTCTCTG GGGGCATGTG GCCAGTTGTG CATATTCAGA AAAAGAACAC TAAAGTCCGT
    GCAAATTTTG GATGCAGACA GTTTGCTTTT GCCGAGGGTC AAGCTCATCG CAATGCAGCA
    GATTTGTGCA TTGATTTGGC TGAAGAGATA AGTGCAAACT TTGAAGCATT GCCCTTTGCT
    ATGGCATCTG ACAGTGATAA TGATGCTGGG ACAAGCATAG CATCTGATCC TGGAACTCAT
    GGACCTCCTT GCCGTATTGC TGCAGTTTCT ACTGTGCAAC AGCAATATGA CAGTGATGCC
    TCATGCCGTT ACAAGGTAGA GCTGAGTTAT CAAGACTTTG TGACCTCTGG ACCTGATCCA
    CACCCACCTC CCATTGCAGA TGATGAAAGT GATGATGATG ACGATGATGA TATTCCTCGA
    GAGGATCATT ATGCTCTTTT GGTAAAAGCA TGGGAGACCA AAGTTTTTCC AACCATTCGA
    AGACGTTTCC GTAACGAGGC AGAGAGAAAG TCTGGCCTGG ACCAGATTAA GGGAGCTCTT
    CAGCTAGGAA TGGTGGATAT AGCGAGGCAA ACTGTAGAGT TCCTCTATGA AGAAAATGGT
    GGCATTCCAA GAGATCTCTA TCTTCCTACT ATTGAGGATA TCAAGGATGA GGCAAATAAA
    TTCACCATTG ATAAAGTTCG CAAAGGACTC ACAGTGGTCA TTCGATCCCC AGATAGCAAC
    AATGTAGCTG GGACAACTGT GGGAACAGCT TTACCCAAGT TTGCCATCCG TGGTATGCTG
    AAGACATTCT CTCTACATGG GGTGGTTCTG GATGTGGATT CAGTTAATGA GCTGGTTCAG
    GTAGAGACTT ACCTCCGGAG TGAAGGGGTA TTAGTGAGAT ACTGGTATCC CATAGATATG
    CTAGAAAGAC CTCCTGCGGG ATACAGGAGA ATGGCAACCA ATGGACTGGT GACTTTAGAT
    AGCACTAACA TCCAGATTCA TAGAGAGCTG TTGCGGTGTG AGTCTGCACT TGCCAGGCTT
    TACTGTCGTG TGGCTTTGCT TAACATCTTT GCTCCTAAGT CTCCGCACAC TTTCACTCGC
    CTGTTCCATA TTCCTGCTAT CCGAGACATT ACATTGGAGC ATTTACAGCT TCTGTCAAAT
    CAGCTTCTAA TGCCACCCCT TCCAGACGGA ACCATTACCT CCAGCTCTAT CCTTCTTGCC
    CAGTCCTTGC AACATTGCAT CTACTCCCAG AACTGCTCAA CAACAGATTT GTTCTACCAG
    GGCAATGCAC AGCCAATAAG AGAGTGGCTT GGTGTGGCCA TAACCAGGGC CCTGCATCAA
    GGAGAGGACA GTCTCTTGGA TCTGACAAAA CAAATCTGTT CCTTCTTGCA AAATGCGCCG
    GAACAGTTTC CATCTGAAGA ATTTCCCATC TCTGAATCCA AGCTCAGTAT GGATGTTAAT
    TTTCCTGGGG CAGCTTTTGT TATTGTTTCT TGTATAGAGA GTCCGTCTGG ATTTAGAAAA
    GACTCCTCCT TGTACAAGGC TCCCTGGTCT AGAGTCATGG TATATGGCCT TGGACATAAA
    GTAAAGAGGA ATGGACAGCT GAACCTGATA GAATCAGTTT GTTATCCACG TGATGCCTCC
    CCAAGTAACA CAGGCCTCAC TCCCCCACCA ACCACAAACC AGTACCCAAC AGCGATTATT
    CCCACTGACA AGGTCCACGT CAAACTTGGT GTATCACCTA CTCCTGGTGC TGTGCTGGTC
    ATCCATTCCC TGCCTCTAGA GTTTCCGTTG GCAATGGCAT TCACAGAACA GCTGTTAACC
    TGGAAGTTGG AGGACACAGA AGAGAAGTCA GAAGATGAGC TTGATACTGT TCCATCCTCT
    GCTCTGTTGC AAGTGGTAGA ACTTTTAGGT ACTTTGATTT GGACAACAGA CCTTGCTGCA
    GTAATTAAGG AGCTTCTGTT CCATCTGTTG GCTGAACTAT TAAGGAAAGT TCACCATCTA
    GAGCAGAAGA AGAACCCAAG TGGATTATCT TCCTCGATAG CCCTGCAGTT GAATCCATGT
    CTAGCAATGC TTATGGCTTT ACAGACAGAA CTGCGCAAGC TGTATGATGA AGAAACTCAA
    AACTGGGCAT CTGGGAATGC ATGTGGGGGG TCTGTACCTG GAGCCTCTGA GCAGGGAAGA
    TTTTCAACTT ACTTTCATGC CCTCATGGAA GTGTGCCTGG CAGTGGCAGA AGTGACGCTC
    CCAATCAACA TGAGCTCAGC AATCAGTGTG ATGGCATCTA CAAATGCCAC TAATCTGAGT
    GACTCTTCTT CTTCCTCTTC CTCTTCCCCT GGACAGACTC CTCAAAGTCC AAGTCTGTTA
    TCAAAGAGAA AGAAAGTTAA GATCAAACGT GAGAAGACTG CATCTGGAAA ACGTACACCT
    TCACGTGGTG CAGAGAGTGA TGCTGCTTTG CTTAGCATTG GAGGAAGCAA ACCTGAGGAC
    ATGTTGTGGT TCCATAGGGC ATTGACTTTA TTGATGATCC TAAGGCATCT AACTAACAAG
    GATCCACAGG GGATGGGTGT CACAAACGAT GCTATTGCTG ATGCTAGCCA GGCTTTAGTT
    GGTCCCACAG CTCACAGTCG TCTCCTTGTG ATTTCTGGCA TCCCTACACA CCTAGAAGAA
    TCAGTGGTCA GAAGTGCAAT TAGAAAGGCA TGTAATGCTC ATGGTGGAGT CTTTAAAGAT
    GAAATCTACA TTCCATTGCA GGAAACTGAA ACCCCTAAAC CCAAAGACAG AATAGAAGGG
    GCAGAATGCA AGACAGAAAT AGAAAAACCC TCTACGCAGC ATACAACCGA CAATATGGAT
    ATCAGCAGCT CGAGCAGTGT AACTCCTGCA GCAAGTGTCA CGGCATCAGC TTCTACAAGC
    CAAGCTTCAT TGTGCAGCTC CCAAGGGGGG GTATCACGGA CCACAAGTGA AACATCTATG
    GAGCAGTTTG CCCCAGAACA TGCTGTGCCA TCAGGGCTTC TGGAACCAAC TATTATCTCC
    AGCCAGGAGA GTCTGGATCT TTCTATGTGC AGCACAGGAA GCCTCGGCAG TCTGGGAAGT
    CTTGTTGAAC AGCTTGATAA TGTTGAAAAC TCTTTGTCTG ACGTGGGCTC CCTATACACA
    GCTACATCAC TAGATAGCCA TATGGCAGAG TCGCGGCCAA TCAAAGGATT TGCAGTTGTA
    GAGGTGCGAT CACGAGCTAA AATAGAGAAA ATCCGTGCCA CTTTATTTAA CAGTAGTGAC
    CTCATTGGGT TATCTGGTTT GGATGGTGAA GATGAGCTGA TGGAAATGTC TACTGAGGAG
    ATCTTGACTG TTTCAACAGT GAATCAGAGC TTGTTTGATA CCCAAGGAAT ACCCGGTCTG
    GCTGATTATT TCATGGATAA ATCAATGAAA GGTGATAAAT TAGTCCCGGA AGCTCGTGAT
    GTTCTGACTG AGATTTTCAA GAGCTGCATT CACGCCGAGC AGATGCTGAG TCTGACTCCA
    GCCAAGTTTA TCAAAGTGTC TGATATCTAC CTTAGCAAGG AGCAAATCAA TTCACAGACC
    CCAGGGAACT TACTGCATAC CTTTTTCACC AATGTTCGAC CACCTAAAAA AGTCCTGGAA
    GATCAACTAA CGCAGATCTT AAGAAAATAT GGAATTCCCA AGCCTAAATT TGACAAAAGC
    AAATACAGCA AAACTGGGAA GGAGCAACAT CCTGTGAAAG TTGTAAGCAC CAAAAGACCT
    GTGGGCAAAC CACCGACCAA AGACAAGGCT GTATTCAACA GCGTCAGGAC TGCTTTAAGT
    GAGAAAAAAC CAACTGTGAA GCCAAAATCT CCGGAGAAGG GCAGGCCAGA TGAGAAGGAC
    CCAGACAAAT CTCCAACAAA GAAGCAAGAA AGGTTTCACT TGTGTCCCTA TCTAGTTCCC
    GAGGAGAGAT ATCTGACGTT GGATGGATTT CACCGCTTTG TAGTAGATCG GGCTAAGCAA
    GACATTCGAA GCATCTGGAG AGCTGTCTTG GCGTGTGGAT ATGATCTTCA TTTTGAAAGG
    TGTGCCTGCA TTGATACAAG GCATGCTCAG AAATCTAGCA GAAAATGGAC CTTGGAAATG
    GATGTTGCTC TTGTTCAGTA TATCAACCGC CTTTGCCGTC ACCTTGCCAT AACGCCAGCC
    CGACTACACC CTCATGAGGT TTATCTGGAT CTTGCAGATA CGGCTGACCC AAGAGTCTCC
    TGCCTGCTGA ATGTACCAAT AGAAAGCCTG CGCCTCCGAT TTGCTCTTCT TCAGTCCCTC
    AACACTACTT TAGAGACCTT TTTCCTGCCC TTAGTGGAGC TACGGCAAAC AGACGTATAC
    ACACACAGCA TCGCATCCCT ACTCCAGCTA GCTAAAGGGT TAATCTTTTA CGATACAAAA
    GTGAATGTGA TGAACCGGGT ATTGAACGCA ACAGTGCAGA GGACAGCAGA TCATGCAGCC
    CCTGAGATCA CGCTGGACCC ACTAGAAATT GTAGGAGGGG AAATTCGTAC CTCAGAAAAC
    TCCTACTTCT GCCAGGCAGC CCGACAGTTG GCATGCATCC CTTCCTCACA ACTGTGTGTC
    AAACTGGCAA GTGGAGGAGA CCCCACGTAT GCATTTAACA TCCGCTTTAC TGGAGAGGAG
    GTGCATGGTA CAAGTGGCTC ATTCCGTCAT TTCCTCTGGC AAGTGTGTAA AGAACTACAG
    AGCTCCTCGC TGTCTCTTCT ACTGCTCTGC CCCAGCTCTG CAGTTAATAA GAATAAGGGA
    AAATATCTTC TAACTCCCAG TCCCATAACA TATGCAGAAG AGCAGTTGCT CCATTTCTTT
    GGCCAACTAC TGGGAATAGC CATCAGAGCC GATGTTCCAC TCCCTCTGGA TCTACTCCCC
    TGCTTCTGGA AGATGCTTGT AGGAGAACCT TTGGACCCAG AAACTGATCT TTATGAAGCA
    GATATTCTCA CCCACAACTA CATCAAGAAG TTTGAAAATG TAAATGATGA GTCAGAGCTG
    GAAGCTCTTT GTGCTGAAAT TGCCTCTCAG CATCTTCCTA CCGAAAGTCC AGATTCTCCT
    CATAAACCAG CCTGCAAATT TACATACTTG ACAATGACTG GTGAAGAAGT GGAGCTGTGC
    CCTAAGGGAA GACACATTTC TGTAGTCTGG GAAAACAAAG ATATATATGC AATGGGAATC
    CGGAACCTTC GTATACGTGA GCTGAAGAAC CAGGAGTGTA TGAGCGCAGT AAGGGCTGGG
    CTGGGCTCCA TTATACCTCT GCAGCTCCTC ACCACTCTAA ATGCTCTAGA GATGGAACTA
    AGGACATGTG GCCTACCATA TATTAATTTG GAATTCTTAA AGGCTCACAC CATGTATCAA
    GTGGGTCTAA TGGAGACAGA TCAACACATT GAGTATTTTT GGTCTGCCTT AGAAATGTTC
    ACTCAGGAAG AACTTTGCAA ATTCATAAAA TTCGCCTGCA ATCAGGAACG CATTCCCTTT
    ACATGTCCAT GCAAAGATGG TGGGCCAGAC ACGGCTCATG TTCCACCTTA TCCTATGAAA
    ATAGCACCAC CCGATGGAGC AACAGGCTCC CCAGATTCCA GGTACATCCG AGTGGAGACA
    TGTATGTTCA TGATCAAGCT TCCGCAGTAC TCGTCTCTAG AGATGATGCT GGAGAAACTG
    AGATATGCCA TTCACTACAG GGAAGACCCA CTCAGCGGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012824247.1
    CDS323..13564
    Translation

    Target ORF information:

    RefSeq Version XM_012968793.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 4 (hectd4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012968793.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGGGGTCGG CGGCTGCCGG GGACTCGGCT CAGTGGCTGT CGGTGAAAGA AGAGACTATC 
    TTTTTACATG ATGGGCTGAT CCGGGTCACA GACCTGGCGG AGTTGCCCAG CGAAATCGGG
    GTCAGTGAGG CCGGGGACGC AGAACTGGAG ATATTGACTT TTGAGACTAA GAACCCTATT
    GAGCTAGCAG AGCATTTACG TGCAGTGTGT GGGAACCAGA GCAATGCCTA TGCACGATTG
    CTGGAATACC GCTTGAATGC GTTACGTGGG TTATGGAATG CCCAACGCCA GCTGGCGCTT
    GAGGATCAAC ATGAAAGAGA TGGGGCTGAT GACGAAACCT TGGCCCTGCT TAAACGGCAG
    GGTTTGTTGC AACAGCCGGA ACAGGCTCCA TTTACATCTC GAATGGGGCT TTTACTGGTG
    TTCCCCCTTA TCCAGTCCCA AAGTAAAACA GACCCTTCAC TTTGTAACTT GACAGCTGAG
    GTGCTTTTAA ATTGCCTTCG GGACTGCCAA CCCCTGAGCT TGACTAAAGA ACCAGCTGAC
    TGCCTGAATG GAATAGAGTC CTTACTGTGT TCTTGGTTAG AAGACACTCC AAATGTGGGC
    CAACAAGTCT CCTTTAAGCA AAAGGAAAAT TCTGCTGCTG CTTTGGTTGC CTTAGCTTGT
    GCTAGGGGAT CATTAAAAAC ATTTATTCAC ACTGTACACT TGCTGCAAAA ACAGACTGAC
    CTAGGATCCC TTCCTGTGGC AGATGTATTG TATAGGTTGC TGCTTTTAGA GGGAGGTCCA
    GGTTCTCCCT CTTGTTTACT GGGAAGCAAA CACATTGTAT CCTGGGGTTT TGAGGATATG
    CTTCCAGCTC CTGATGGGAT CAGTGGTTCA AACAATGAGA ACAAAGATGC AGATTTGGGT
    CGCTGTGTAG CATGCGATGG GCTTTATCTT TACACAACTA ACTCTGTAGG TAGAGGATTG
    AGCAAAGTAG GGTCTGGGCT GCATGGCACT TTACGAGGTT TTGTTTATTC CCGAAACGAG
    GAGCTAGAGG CTGGCTGGAT TGCTTATGGA AGTGGGAAGA TACTTCACAG GCCTGCCTCC
    TTTGATAACA AACCTCAATA TCTTTTCCAA TTAATTGACC AATACACACT TCAGGTCTCT
    TATATAGTCA GCATGCCAGC CAATCATTTC CCGGTTGGCA GCAATATGAC TACGGTGCAC
    CTCTCCTCGG ATGGCACGTA TTTTTACTGG ATTTGGTCTC CAGCTAGTTT AAATGAGAAA
    ACCCCCAAAG GACACTCTGT CTTCATGGAT ATATTTGAGT TAAATGTGCA AAATGGAGTA
    TGCATTGCAA GCCCTCTTCA TGAGCGCATT ATATTAATGA GGAAAGAGGG AGAATCTGCT
    AAAAGTATTA ATGAAATGCT TTTGAGCAGA TTATCACGAT ACAGAGCTTC TCCTTCAGCA
    ACTCTAGCAG CACTTACAGG ATCTACAATC TCTAACACTC TAAAAGAGGA CCAGACTGCT
    GCAAATACGA GCTGCGGGCT TCCTTTGAAA ATGTTAAGAA AGACTCCAAT GTATACTTGT
    GGCACATACT TAGTACTACT CGTGCCACCT CCGGGTGGGT CAGGAAGTTC AGCAACAAGA
    TCTTTGTTTG GTGGAACCAG CGGCCTCTCC TCTTTAAAAA TTCTGGCTTC CAGCTTGGTA
    TTTAACATTT CTGATGGGCA GTTCACCAAC AGAGCAGATC TTATTGATGC ATCTGGAAGT
    TCTCTTGGAA GGGGTGCTTT GGTGACAGGC CTAGGTGCCT GCTATGATAC AATGAATAAC
    ATGATCTGGA CGTGCTCCAA TGATTACATC GATCAGTGGT GTAATCCTGG AAACCAGGCT
    TTCCATGTGG TGTGTCAGAG GCTTGGTGTC ACTCACATCA TTGCTGATCC CAAAGGAGAG
    GCAGTATCAA CAAATGAAGT TATAAACCAG TTGCTTCACC ATGTGGGTGC TATGTGTATT
    CACCAACTCA ATCTCCTGGC TAATAGCAAC AGTTCTCCAA TCAGTACCTT TCTGGGCAAA
    CAACATCCTA TAGAGCCACA TCATCTTAAT AGTATATGTG ACATCATGGA GAAAGCCATG
    GTCAATGGTG ATAAATGCAT TATTCGCTGC ATCTTGGTTG TTTTCCAGGT GGTATTTAAA
    TTCTTTCTAA ACCCACAAAC TGAGAGGAAT CGAGAAACCA TAAGGAGGGC TGGTTTGCTC
    CTTTGGCAGT TGCTCATGGC GCCTAAGGAC CAAATTTGTC CTGAAATACA GAGAGAGGTC
    TGCTTGGCTA TTAGCTTTGG TTTAAACATT TTGAATCCAG GAGAGCTAGA AATTAATAAC
    TTGCTCAAGC TGATTTTAAC TGAAGGGGAC AGAAATTGTG GCCTCTCCCA ACTCCGTGAT
    GTCATTTTGA CAAACCTCAC TGAACAGCTT CAGAATAACA GGTTTGGAAG TGAAGATGAT
    GAACATTACA GGCTTAATGA TGAACTATTG CACTATATTT TGAAGATTGT TGTTCGTGAA
    TCCTGTGTTC TGATCACCAA GTGCCAAACA GTGTCTAAAG AAGAATTCCA AAGACTCTTG
    TCAACAGTGC CTGTGGCTTC TCCCTGTCTA TGCTATTTAA TGGCTGTACA GAATCATCTC
    CTGAGTAACA CTGTCTTAAT TAAACCTGAT GACAGCGATG ACAGTGACAA CTCCCTGCAG
    GGGGAGACAT TGAAGGTACA GGAACTCCAG GCCAGCATCT TGGCTTTGGC AACCCAGATA
    CTTTCTGGTT GTGATGAAGT TCTGGATATT CTGCAGCGAG TGACCACTGC TCTTATCAAC
    AGCGACCTGT TAGACAGAGA TTTAAGACTG AAAGGCCTTG AACAGATCAC AAAGTCCACC
    CTGCTTGGCC ATCTCCTCCC AGTACTTCTC ACATCCCTTA TGCATCCAAA CTTACAGACA
    TTAACCATGG CAGATGCGCT TATGCCTCAG CTGGTGCAGC TTGTTCTTTA CACTAGCCAG
    ACTGCACTGC TGCTTAAAAC CCAGTGTCCA GTATTGGCAG ATACAGGGGA CACCACCAGT
    GGAATTCCAG AGCAAAAAAA CAAACTGTTA ACTGATGACA GGATGCTGGA AGAAAAGGAG
    GAGCCTGGTT TCCTCACTGG GCTAAAGATT CCTGCGCCAT GGGCTGCAGG AAAGACTGTG
    GAAACATCTC ATCCAGTCAG AGACAATTAC AAGTTTAAAG AGACTGTGCA CATTCCTGGA
    GCTCGTTGTT TGTACCTCCG GTTTGACAGC AGGTGCTCTT CACAGTACGA TTATGATAAG
    CTGGTGATAT ATGCGGGACC CAACACAAAC AGCAGAAAAG TTGCCGAATA TGGTGGAAAT
    ACCCTGGGGT ATGGCAGTAG AAGTGTTTTA GGTACAGGCT GGCCAAAAGA TTTGGTTAAG
    GTGGAAGGGG ATACTGTGAC ATTCTCCTTC GAAATGAGAA GTGGGCGTGA GCACAACACT
    CCTGATAAAG CAATGTGGGG TTTTGCGTGC ACTGTCAGAG CTCAGGAGTC CTCAGAAGAT
    GTGTCAGGGG GCTTGCCATT TCTAGTAGAC CTGGCTTTAG GTCTGTCTGT CTTGGCTTGT
    TCCATGCTGC GTATATTGTA CAATGGTCCA GAAATCACAA AAGAAGAGTT GACTTGTCAC
    GATCTGTTGA GGTCAAAGCT TTTGCAGAGG TGTCAGTGGC AGGTGGAGGC TAATGGTGCT
    TTATCCCCGG CACTCACCCC AAGCCCTTCG CCGCTGCCCC TTACTATAGA AGAAGACCAA
    GAATTTACGT ATCCATCTGA CATCTTGTTG CCACCAGTTG GGAACTATTT TGAACTGCCA
    AGAATCAGAC TGCCTCCTGG TATTATGATC AAGCTCAGGG AAATATCTGG ACGCGCTCGA
    CCACAGTTTA GACCAAGCAT AAAAGAGGTT ATCCAGCCTG ATGTGATGGA AGAAATGGTT
    GTATCTTGCG TCATTAAACA CTTGAACCTG GTCGATGCAT TGCAGTCTTT AATAAACTTC
    CAGTATCGAG AAGAACATCT AGAAGAATAT GATTTATTAT GTAAAATAAT GGGGGAAACT
    TTTAAGAAGC TCAATGCAAT GGAAAGACAA TTGCAGAGTG TTGCAGAACT GGAACAAAAG
    TGGCAAAGTG AAGTGGAAGA TGCTCAGAAT GGAAAGCTGG AGAACAACGC TCCCTTTTTT
    TACGACTATC ATTTTAATGA GAGCAAGTTA AAGGAACTTG AACTTTTATG TTCTATGAAA
    GAGGTCCCTT CTGATGGAAG TGATCCAGAA AACATGGTTT TCTCTCTGCG AGAGAAGTTT
    TTTCAAGAGG TGAACTCCCT GATCCAAAAA CCTTCCCACC CAATGGCTAA GACCAAAACA
    TTGGTTAAGA GTCTGATGAA TAGAGCAGAG CTCCTGTTAC ATGTGACTAT TGCAGCACAT
    TCTGGTATCA CGAGAAGCAT ATCTGGCACT CCAACTGAAC CTCAAGCTGG CAAGTCTATC
    TCAGAAGCAA AAGTTATATC TCAAGCCATG CGCCAGCCTG TTTTCCTCCG CAGCATGTCT
    GCCCCTTCTG ACCTGGAAAT GATTGGAAGT AGTGACTTAG AGTTCAGCAG GTCTAATCCA
    AGGAGAAGGA ACATCACTAG CCACAGGAGC AGCTCCTTCA CTCTGCTTCA GTCTTTAGCT
    ATTGAAGACT GCAAGGATAA ACCAACATAC AGTGTCCTTT TAGGGCAACT GTTTGCCTTC
    ATTGGGACCA ATCCCGACCA AGCAGTATCC AGTAGCAGCT TTCTACTTGC AGCCCAAACC
    AGATGGCGTC GTGGTAATAC TCGCAAACAA GCCCTTGTAC ACATGAGAGA ATTACTGACT
    GCTGCTGTCC GTGTTGGGGG TGTGACACAT TTGGTTGGAC CAGTAACAAT GGTGCTACAG
    GGAGGACCAA GAATTGAAGA GCTCACCTGT GGAGGAATGG TGGAGCTTGT TCAAGAGGCA
    TTTGGAGAGA CCATGACCTC AGTGGTGTCA CTTTGTGCAC GGTATCCCAT TGCCTGTGCA
    AATAGCATTG GGCTTTTGTG TACTATACCT TACACAAGGA GTGAAGAAAA ATGTTTAGTT
    CGCAGTGGGC TTGTTCAACT GATGGACCGA CTCTGCAGCT TAAGCAACCA GAGAGAGTCC
    AGCTCAAATG AAAAGCAAAC CAAGAAACAG AAAGTAGCAA CTATGGCATG GGCAGCCTTC
    CAAGTACTGG CCAATAGATG TGTAGAATGG GAGAAAGAAG AAGGTGGTTC TACTGAGGCG
    GTACACTCTG GTTTGGCTAG ACAAGTCTCC ACCCTCCTCA CTAACCATCT AGCAAGGGCT
    ACAGAGTGCT GTGGAAACCA AGCTGCAGGG AATGATGCCC TACAGGACGT TCTGAGCCTT
    CTCAATGATC TCTCTAGGAG TCAGATAGGG AAGGCAATTC TGAGCCAGCC TGCTTGTGTG
    TCCAAACTTC TTTCGTTGCT GCTGGACCAG CGTCCTTCTC CAAAGCTTGT GCTCATAATT
    CTGCAGCTGT GCCGCGCTGC ACTTCCTCTC ATGAGTGTTG AAGACTGTGG AAATGTAGAA
    CTTCCTCCAT GGAGTTACTC TTTACCAGCT CTGGACAGTG AACAGGGGGA GGATCCAAGT
    GACCCAGCCT CCAAAATAGC ATCTTTGTTG CTAGCAAAGT TGGCTGATTA TGTTGTTCCA
    GGATGTCAGA CAGTTCTTTC TCCTACTGCT TCTGAACCAG ACAGCTCATT GTCCAAAAAC
    AGCCCAAAAA ATTCCCAGAA AGCAGATAAG GATGCAGAAG AAGAGAGTGA AGCAGTAGAT
    GGCAAGCTCT CCATTTTTAT TCATAAGCGA GAAGACCAGT CATCCCATGA GGTTCTTCAG
    CCTTTGCTTA GTAGTTCAGA GGGAAGGCCT TTTCGTCTTG GTACTGGAGC GAATATGGAA
    AAAGTAGTAA AGATGGATCG TGACATGACT AAGAGTGGAT GTTGTGAAGT AATCACAGAG
    GAGGCTGCAG CTGCTCTCCG CAAAGCCACT AAATGGGCCC AGTCGGGTTT AATTGTTAGC
    ATTGGGCCAC CTGTGGAGAC AGTTAGCCCT GATTCAGCGA GTGGACTTTC CACTGGTGAC
    AAAAAGAAAA CAGCCCAGAC CTCGATCTGT AGGGAAAGGA ATTCTGAACT TGCCAGGACT
    GATCCTGTAC GCCCTTTCAT TAGTGGCCAT GTAGCAAACA GCATGGCTGC AGAAGTAATT
    GCTCTGCTTC ACAGCCTGCT GATGGCACCA GAGTCAAATG CTGCCCAAAT CTGGACAACA
    ACAGCAGAGA AGGTTCTTTC TCGTGCCCTG ATGTATATCC CACAGCTTGG CAAATATGCA
    GAAAGCATCC TAGAGAACGG CAGCGGTAAT GGCAGGAAGC TAGCAAAGCT ACAAGGCATT
    GCAAGGCAAG CAGTTGCTGC ATTATGTGCG CTTGGTGGAT TTAAAGAAAC CATTAAAATA
    GGTTCTGAAG TCCAGGTGTT GGGTAAAGGA ATATCTGGGA GCATTGGAGT GGTTGTTTCT
    ATTAATGAAC AAGAAGGTAT TGCAACAGTG AAATTTCCAG ACATAGATTT TAGAAAATCA
    TGCAAAGCAT CTGACACACT GACAGTACCA TTATTTAGGC TCTGCGTTCC ACGGTCAGAG
    GCATTGCCTC TCCATAAACT GTCCATTACT GAGAAGGTAG TGCAGGCAGT CCAGTCCATG
    TTACTTCCCC AGGAGGGCTG CCTATCTATT CATACCTCGC TTCCTGCAAC AGGAGATGCC
    TCAACTCCAG TAATGGCAGT GGTTCGACTT CTTGCAGAGA TCAGGACAAG AGCTTGCTTG
    GTGATGGCTC AGCTGTTGGA GGACAGCTCA TTTTGTGAAG AATTCATACA GCAATGTCCA
    GCTGCTGTAG AAGTTCTAAA CCTAGTGGCC CAGGAATGCA GTCCTGGGGA GCGTCTCTCA
    GTGGTGGAAG TTCAGTGTGA AAGACTACGA ATGCTTTATC GGGACTGTGC TCGCCCCCCG
    CCACCACCTT TGCAGGCTGA GCGCAGACAG CCCAAGGAAA TCACCTGGTC TCCTTCCAGG
    GTATTCCCTC CTGTGAGAGC CTGTATGTTT TCATCCCATC TAACAGCAGT AACATTTCTG
    GCTGACCCAA GTGCTGGTGG GGGACTTCCT CGCGGCACAT TTATATATGC AACATCCCCT
    GTTCCAGTAC AGGCACCATC TTTTTACTGG GAAATAGAAG TAGTTTCCTA TGGAGACACA
    GATGATGACA CTGGCCCTAT TGTATCATTT GGGTTTGCTA CAGAAGCAGA GAAGCGGGAT
    GGAGCATGGA CAAACCCAGT CGGTACCTGT CTTTTTCATA ACAATGGTCG AGCTGTACAT
    TACAATGGAT CAAGTCTCTT ACAGTGGAAG AGTGTTCGAT TAGATGTGAC TCTTGCTCCT
    GGTGATGTTG CTGGAATAGG TTGGGAGAGA ACTGAAGGAA CACCACCACC CCCAGGGCAG
    CCTGCCAAGG GCAGAGTCTA CTTTACTTAC TGTGGACAGA GGCTTACTCC GTTCTTAGAG
    GATGTCTCTG GGGGCATGTG GCCAGTTGTG CATATTCAGA AAAAGAACAC TAAAGTCCGT
    GCAAATTTTG GATGCAGACA GTTTGCTTTT GCCGAGGGTC AAGCTCATCG CAATGCAGCA
    GATTTGTGCA TTGATTTGGC TGAAGAGATA AGTGCAAACT TTGAAGCATT GCCCTTTGCT
    ATGGCATCTG ACAGTGATAA TGATGCTGGG ACAAGCATAG CATCTGATCC TGGAACTCAT
    GGACCTCCTT GCCGTATTGC TGCAGTTTCT ACTGTGCAAC AGCAATATGA CAGTGATGCC
    TCATGCCGTT ACAAGGTAGA GCTGAGTTAT CAAGACTTTG TGACCTCTGG ACCTGATCCA
    CACCCACCTC CCATTGCAGA TGATGAAAGT GATGATGATG ACGATGATGA TATTCCTCGA
    GAGGATCATT ATGCTCTTTT GGTAAAAGCA TGGGAGACCA AAGTTTTTCC AACCATTCGA
    AGACGTTTCC GTAACGAGGC AGAGAGAAAG TCTGGCCTGG ACCAGATTAA GGGAGCTCTT
    CAGCTAGGAA TGGTGGATAT AGCGAGGCAA ACTGTAGAGT TCCTCTATGA AGAAAATGGT
    GGCATTCCAA GAGATCTCTA TCTTCCTACT ATTGAGGATA TCAAGGATGA GGCAAATAAA
    TTCACCATTG ATAAAGTTCG CAAAGGACTC ACAGTGGTCA TTCGATCCCC AGATAGCAAC
    AATGTAGCTG GGACAACTGT GGGAACAGCT TTACCCAAGT TTGCCATCCG TGGTATGCTG
    AAGACATTCT CTCTACATGG GGTGGTTCTG GATGTGGATT CAGTTAATGA GCTGGTTCAG
    GTAGAGACTT ACCTCCGGAG TGAAGGGGTA TTAGTGAGAT ACTGGTATCC CATAGATATG
    CTAGAAAGAC CTCCTGCGGG ATACAGGAGA ATGGCAACCA ATGGACTGGT GACTTTAGAT
    AGCACTAACA TCCAGATTCA TAGAGAGCTG TTGCGGTGTG AGTCTGCACT TGCCAGGCTT
    TACTGTCGTG TGGCTTTGCT TAACATCTTT GCTCCTAAGT CTCCGCACAC TTTCACTCGC
    CTGTTCCATA TTCCTGCTAT CCGAGACATT ACATTGGAGC ATTTACAGCT TCTGTCAAAT
    CAGCTTCTAA TGCCACCCCT TCCAGACGGA ACCATTACCT CCAGCTCTAT CCTTCTTGCC
    CAGTCCTTGC AACATTGCAT CTACTCCCAG AACTGCTCAA CAACAGATTT GTTCTACCAG
    GGCAATGCAC AGCCAATAAG AGAGTGGCTT GGTGTGGCCA TAACCAGGGC CCTGCATCAA
    GGAGAGGACA GTCTCTTGGA TCTGACAAAA CAAATCTGTT CCTTCTTGCA AAATGCGCCG
    GAACAGTTTC CATCTGAAGA ATTTCCCATC TCTGAATCCA AGCTCAGTAT GGATGTTAAT
    TTTCCTGGGG CAGCTTTTGT TATTGTTTCT TGTATAGAGA GTCCGTCTGG ATTTAGAAAA
    GACTCCTCCT TGTACAAGGC TCCCTGGTCT AGAGTCATGG TATATGGCCT TGGACATAAA
    GTAAAGAGGA ATGGACAGCT GAACCTGATA GAATCAGTTT GTTATCCACG TGATGCCTCC
    CCAAGTAACA CAGGCCTCAC TCCCCCACCA ACCACAAACC AGTACCCAAC AGCGATTATT
    CCCACTGACA AGGTCCACGT CAAACTTGGT GTATCACCTA CTCCTGGTGC TGTGCTGGTC
    ATCCATTCCC TGCCTCTAGA GTTTCCGTTG GCAATGGCAT TCACAGAACA GCTGTTAACC
    TGGAAGTTGG AGGACACAGA AGAGAAGTCA GAAGATGAGC TTGATACTGT TCCATCCTCT
    GCTCTGTTGC AAGTGGTAGA ACTTTTAGGT ACTTTGATTT GGACAACAGA CCTTGCTGCA
    GTAATTAAGG AGCTTCTGTT CCATCTGTTG GCTGAACTAT TAAGGAAAGT TCACCATCTA
    GAGCAGAAGA AGAACCCAAG TGGATTATCT TCCTCGATAG CCCTGCAGTT GAATCCATGT
    CTAGCAATGC TTATGGCTTT ACAGACAGAA CTGCGCAAGC TGTATGATGA AGAAACTCAA
    AACTGGGCAT CTGGGAATGC ATGTGGGGGG TCTGTACCTG GAGCCTCTGA GCAGGGAAGA
    TTTTCAACTT ACTTTCATGC CCTCATGGAA GTGTGCCTGG CAGTGGCAGA AGTGACGCTC
    CCAATCAACA TGAGCTCAGC AATCAGTGTG ATGGCATCTA CAAATGCCAC TAATCTGAGT
    GACTCTTCTT CTTCCTCTTC CTCTTCCCCT GGACAGACTC CTCAAAGTCC AAGTCTGTTA
    TCAAAGAGAA AGAAAGTTAA GATCAAACGT GAGAAGACTG CATCTGGAAA ACGTACACCT
    TCACGTGGTG CAGAGAGTGA TGCTGCTTTG CTTAGCATTG GAGGAAGCAA ACCTGAGGAC
    ATGTTGTGGT TCCATAGGGC ATTGACTTTA TTGATGATCC TAAGGCATCT AACTAACAAG
    GATCCACAGG GGATGGGTGT CACAAACGAT GCTATTGCTG ATGCTAGCCA GGCTTTAGTT
    GGTCCCACAG CTCACAGTCG TCTCCTTGTG ATTTCTGGCA TCCCTACACA CCTAGAAGAA
    TCAGTGGTCA GAAGTGCAAT TAGAAAGGCA TGTAATGCTC ATGGTGGAGT CTTTAAAGAT
    GAAATCTACA TTCCATTGCA GGAAACTGAA ACCCCTAAAC CCAAAGACAG AATAGAAGGG
    GCAGAATGCA AGACAGAAAT AGAAAAACCC TCTACGCAGC ATACAACCGA CAATATGGAT
    ATCAGCAGCT CGAGCAGTGT AACTCCTGCA GCAAGTGTCA CGGCATCAGC TTCTACAAGC
    CAAGCTTCAT TGTGCAGCTC CCAAGGGGGG GTATCACGGA CCACAAGTGA AACATCTATG
    GAGCAGTTTG CCCCAGAACA TGCTGTGCCA TCAGGGCTTC TGGAACCAAC TATTATCTCC
    AGCCAGGAGA GTCTGGATCT TTCTATGTGC AGCACAGGAA GCCTCGGCAG TCTGGGAAGT
    CTTGTTGAAC AGCTTGATAA TGTTGAAAAC TCTTTGTCTG ACGTGGGCTC CCTATACACA
    GCTACATCAC TAGATAGCCA TATGGCAGAG TCGCGGCCAA TCAAAGGATT TGCAGTTGTA
    GAGGTGCGAT CACGAGCTAA AATAGAGAAA ATCCGTGCCA CTTTATTTAA CAGTAGTGAC
    CTCATTGGGT TATCTGGTTT GGATGGTGAA GATGAGCTGA TGGAAATGTC TACTGAGGAG
    ATCTTGACTG TTTCAACAGT GAATCAGAGC TTGTTTGATA CCCAAGGAAT ACCCGGTCTG
    GCTGATTATT TCATGGATAA ATCAATGAAA GGTGATAAAT TAGTCCCGGA AGCTCGTGAT
    GTTCTGACTG AGATTTTCAA GAGCTGCATT CACGCCGAGC AGATGCTGAG TCTGACTCCA
    GCCAAGTTTA TCAAAGTGTC TGATATCTAC CTTAGCAAGG AGCAAATCAA TTCACAGACC
    CCAGGGAACT TACTGCATAC CTTTTTCACC AATGTTCGAC CACCTAAAAA AGTCCTGGAA
    GATCAACTAA CGCAGATCTT AAGAAAATAT GGAATTCCCA AGCCTAAATT TGACAAAAGC
    AAATACAGCA AAACTGGGAA GGAGCAACAT CCTGTGAAAG TTGTAAGCAC CAAAAGACCT
    GTGGGCAAAC CACCGACCAA AGACAAGGCT GTATTCAACA GCGTCAGGAC TGCTTTAAGT
    GAGAAAAAAC CAACTGTGAA GCCAAAATCT CCGGAGAAGG GCAGGCCAGA TGAGAAGGAC
    CCAGACAAAT CTCCAACAAA GAAGCAAGAA AGGTTTCACT TGTGTCCCTA TCTAGTTCCC
    GAGGAGAGAT ATCTGACGTT GGATGGATTT CACCGCTTTG TAGTAGATCG GGCTAAGCAA
    GACATTCGAA GCATCTGGAG AGCTGTCTTG GCGTGTGGAT ATGATCTTCA TTTTGAAAGG
    TGTGCCTGCA TTGATACAAG GCATGCTCAG AAATCTAGCA GAAAATGGAC CTTGGAAATG
    GATGTTGCTC TTGTTCAGTA TATCAACCGC CTTTGCCGTC ACCTTGCCAT AACGCCAGCC
    CGACTACACC CTCATGAGGT TTATCTGGAT CTTGCAGATA CGGCTGACCC AAGAGTCTCC
    TGCCTGCTGA ATGTACCAAT AGAAAGCCTG CGCCTCCGAT TTGCTCTTCT TCAGTCCCTC
    AACACTACTT TAGAGACCTT TTTCCTGCCC TTAGTGGAGC TACGGCAAAC AGACGTATAC
    ACACACAGCA TCGCATCCCT ACTCCAGCTA GCTAAAGGGT TAATCTTTTA CGATACAAAA
    GTGAATGTGA TGAACCGGGT ATTGAACGCA ACAGTGCAGA GGACAGCAGA TCATGCAGCC
    CCTGAGATCA CGCTGGACCC ACTAGAAATT GTAGGAGGGG AAATTCGTAC CTCAGAAAAC
    TCCTACTTCT GCCAGGCAGC CCGACAGTTG GCATGCATCC CTTCCTCACA ACTGTGTGTC
    AAACTGGCAA GTGGAGGAGA CCCCACGTAT GCATTTAACA TCCGCTTTAC TGGAGAGGAG
    GTGCATGGTA CAAGTGGCTC ATTCCGTCAT TTCCTCTGGC AAGTGTGTAA AGAACTACAG
    AGCTCCTCGC TGTCTCTTCT ACTGCTCTGC CCCAGCTCTG CAGTTAATAA GAATAAGGGA
    AAATATCTTC TAACTCCCAG TCCCATAACA TATGCAGAAG AGCAGTTGCT CCATTTCTTT
    GGCCAACTAC TGGGAATAGC CATCAGAGCC GATGTTCCAC TCCCTCTGGA TCTACTCCCC
    TGCTTCTGGA AGATGCTTGT AGGAGAACCT TTGGACCCAG AAACTGATCT TTATGAAGCA
    GATATTCTCA CCCACAACTA CATCAAGAAG TTTGAAAATG TAAATGATGA GTCAGAGCTG
    GAAGCTCTTT GTGCTGAAAT TGCCTCTCAG CATCTTCCTA CCGAAAGTCC AGATTCTCCT
    CATAAACCAG CCTGCAAATT TACATACTTG ACAATGACTG GTGAAGAAGT GGAGCTGTGC
    CCTAAGGGAA GACACATTTC TGTAGTCTGG GAAAACAAAG ATATATATGC AATGGGAATC
    CGGAACCTTC GTATACGTGA GCTGAAGAAC CAGGAGTGTA TGAGCGCAGT AAGGGCTGGG
    CTGGGCTCCA TTATACCTCT GCAGCTCCTC ACCACTCTAA ATGCTCTAGA GATGGAACTA
    AGGACATGTG GCCTACCATA TATTAATTTG GAATTCTTAA AGGCTCACAC CATGTATCAA
    GTGGGTCTAA TGGAGACAGA TCAACACATT GAGTATTTTT GGTCTGCCTT AGAAATGTTC
    ACTCAGGAAG AACTTTGCAA ATTCATAAAA TTCGCCTGCA ATCAGGAACG CATTCCCTTT
    ACATGTCCAT GCAAAGATGG TGGGCCAGAC ACGGCTCATG TTCCACCTTA TCCTATGAAA
    ATAGCACCAC CCGATGGAGC AACAGGCTCC CCAGATTCCA GGTACATCCG AGTGGAGACA
    TGTATGTTCA TGATCAAGCT TCCGCAGTAC TCGTCTCTAG AGATGATGCT GGAGAAACTG
    AGATATGCCA TTCACTACAG GGAAGACCCA CTCAGCGGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31529
    Clone ID Related Accession (Same CDS sequence) XM_012968794.2
    Accession Version XM_012968794.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13236bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product probable E3 ubiquitin-protein ligase HECTD4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968794.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGGGGTCGG CGGCTGCCGG GGACTCGGCT CAGTGGCTGT CGGTGAAAGA AGAGACTATC 
    TTTTTACATG ATGGGCTGAT CCGGGTCACA GACCTGGCGG AGTTGCCCAG CGAAATCGGG
    GTCAGTGAGG CCGGGGACGC AGAACTGGAG ATATTGACTT TTGAGACTAA GAACCCTATT
    GAGCTAGCAG AGCATTTACG TGCAGTGTGT GGGAACCAGA GCAATGCCTA TGCACGATTG
    CTGGAATACC GCTTGAATGC GTTACGTGGG TTATGGAATG CCCAACGCCA GCTGGCGCTT
    GAGGATCAAC ATGAAAGAGA TGGGGCTGAT GACGAAACCT TGGCCCTGCT TAAACGGCAG
    GGTTTGTTGC AACAGCCGGA ACAGGCTCCA TTTACATCTC GAATGGGGCT TTTACTGGTG
    TTCCCCCTTA TCCAGTCCCA AAGTAAAACA GACCCTTCAC TTTGTAACTT GACAGCTGAG
    GTGCTTTTAA ATTGCCTTCG GGACTGCCAA CCCCTGAGCT TGACTAAAGA ACCAGCTGAC
    TGCCTGAATG GAATAGAGTC CTTACTGTGT TCTTGGTTAG AAGACACTCC AAATGTGGGC
    CAACAAGTCT CCTTTAAGCA AAAGGAAAAT TCTGCTGCTG CTTTGGTTGC CTTAGCTTGT
    GCTAGGGGAT CATTAAAAAC ATTTATTCAC ACTGTACACT TGCTGCAAAA ACAGACTGAC
    CTAGGATCCC TTCCTGTGGC AGATGTATTG TATAGGTTGC TGCTTTTAGA GGGAGGTCCA
    GGTTCTCCCT CTTGTTTACT GGGAAGCAAA CACATTGTAT CCTGGGGTTT TGAGGATATG
    CTTCCAGCTC CTGATGGGAT CAGTGGTTCA AACAATGAGA ACAAAGATGC AGATTTGGGT
    CGCTGTGTAG CATGCGATGG GCTTTATCTT TACACAACTA ACTCTGTAGG TAGAGGATTG
    AGCAAAGTAG GGTCTGGGCT GCATGGCACT TTACGAGGTT TTGTTTATTC CCGAAACGAG
    GAGCTAGAGG CTGGCTGGAT TGCTTATGGA AGTGGGAAGA TACTTCACAG GCCTGCCTCC
    TTTGATAACA AACCTCAATA TCTTTTCCAA TTAATTGACC AATACACACT TCAGGTCTCT
    TATATAGTCA GCATGCCAGC CAATCATTTC CCGGTTGGCA GCAATATGAC TACGGTGCAC
    CTCTCCTCGG ATGGCACGTA TTTTTACTGG ATTTGGTCTC CAGCTAGTTT AAATGAGAAA
    ACCCCCAAAG GACACTCTGT CTTCATGGAT ATATTTGAGT TAAATGTGCA AAATGGAGTA
    TGCATTGCAA GCCCTCTTCA TGAGCGCATT ATATTAATGA GGAAAGAGGG AGAATCTGCT
    AAAAGTATTA ATGAAATGCT TTTGAGCAGA TTATCACGAT ACAGAGCTTC TCCTTCAGCA
    ACTCTAGCAG CACTTACAGG ATCTACAATC TCTAACACTC TAAAAGAGGA CCAGACTGCT
    GCAAATACGA GCTGCGGGCT TCCTTTGAAA ATGTTAAGAA AGACTCCAAT GTATACTTGT
    GGCACATACT TAGTACTACT CGTGCCACCT CCGGGTGGGT CAGGAAGTTC AGCAACAAGA
    TCTTTGTTTG GTGGAACCAG CGGCCTCTCC TCTTTAAAAA TTCTGGCTTC CAGCTTGGTA
    TTTAACATTT CTGATGGGCA GTTCACCAAC AGAGCAGATC TTATTGATGC ATCTGGAAGT
    TCTCTTGGAA GGGGTGCTTT GGTGACAGGC CTAGGTGCCT GCTATGATAC AATGAATAAC
    ATGATCTGGA CGTGCTCCAA TGATTACATC GATCAGTGGT GTAATCCTGG AAACCAGGCT
    TTCCATGTGG TGTGTCAGAG GCTTGGTGTC ACTCACATCA TTGCTGATCC CAAAGGAGAG
    GCAGTATCAA CAAATGAAGT TATAAACCAG TTGCTTCACC ATGTGGGTGC TATGTGTATT
    CACCAACTCA ATCTCCTGGC TAATAGCAAC AGTTCTCCAA TCAGTACCTT TCTGGGCAAA
    CAACATCCTA TAGAGCCACA TCATCTTAAT AGTATATGTG ACATCATGGA GAAAGCCATG
    GTCAATGGTG ATAAATGCAT TATTCGCTGC ATCTTGGTTG TTTTCCAGGT GGTATTTAAA
    TTCTTTCTAA ACCCACAAAC TGAGAGGAAT CGAGAAACCA TAAGGAGGGC TGGTTTGCTC
    CTTTGGCAGT TGCTCATGGC GCCTAAGGAC CAAATTTGTC CTGAAATACA GAGAGAGGTC
    TGCTTGGCTA TTAGCTTTGG TTTAAACATT TTGAATCCAG GAGAGCTAGA AATTAATAAC
    TTGCTCAAGC TGATTTTAAC TGAAGGGGAC AGAAATTGTG GCCTCTCCCA ACTCCGTGAT
    GTCATTTTGA CAAACCTCAC TGAACAGCTT CAGAATAACA GGTTTGGAAG TGAAGATGAT
    GAACATTACA GGCTTAATGA TGAACTATTG CACTATATTT TGAAGATTGT TGTTCGTGAA
    TCCTGTGTTC TGATCACCAA GTGCCAAACA GTGTCTAAAG AAGAATTCCA AAGACTCTTG
    TCAACAGTGC CTGTGGCTTC TCCCTGTCTA TGCTATTTAA TGGCTGTACA GAATCATCTC
    CTGAGTAACA CTGTCTTAAT TAAACCTGAT GACAGCGATG ACAGTGACAA CTCCCTGCAG
    GGGGAGACAT TGAAGGAACT CCAGGCCAGC ATCTTGGCTT TGGCAACCCA GATACTTTCT
    GGTTGTGATG AAGTTCTGGA TATTCTGCAG CGAGTGACCA CTGCTCTTAT CAACAGCGAC
    CTGTTAGACA GAGATTTAAG ACTGAAAGGC CTTGAACAGA TCACAAAGTC CACCCTGCTT
    GGCCATCTCC TCCCAGTACT TCTCACATCC CTTATGCATC CAAACTTACA GACATTAACC
    ATGGCAGATG CGCTTATGCC TCAGCTGGTG CAGCTTGTTC TTTACACTAG CCAGACTGCA
    CTGCTGCTTA AAACCCAGTG TCCAGTATTG GCAGATACAG GGGACACCAC CAGTGGAATT
    CCAGAGCAAA AAAACAAACT GTTAACTGAT GACAGGATGC TGGAAGAAAA GGAGGAGCCT
    GGTTTCCTCA CTGGGCTAAA GATTCCTGCG CCATGGGCTG CAGGAAAGAC TGTGGAAACA
    TCTCATCCAG TCAGAGACAA TTACAAGTTT AAAGAGACTG TGCACATTCC TGGAGCTCGT
    TGTTTGTACC TCCGGTTTGA CAGCAGGTGC TCTTCACAGT ACGATTATGA TAAGCTGGTG
    ATATATGCGG GACCCAACAC AAACAGCAGA AAAGTTGCCG AATATGGTGG AAATACCCTG
    GGGTATGGCA GTAGAAGTGT TTTAGGTACA GGCTGGCCAA AAGATTTGGT TAAGGTGGAA
    GGGGATACTG TGACATTCTC CTTCGAAATG AGAAGTGGGC GTGAGCACAA CACTCCTGAT
    AAAGCAATGT GGGGTTTTGC GTGCACTGTC AGAGCTCAGG AGTCCTCAGA AGATGTGTCA
    GGGGGCTTGC CATTTCTAGT AGACCTGGCT TTAGGTCTGT CTGTCTTGGC TTGTTCCATG
    CTGCGTATAT TGTACAATGG TCCAGAAATC ACAAAAGAAG AGTTGACTTG TCACGATCTG
    TTGAGGTCAA AGCTTTTGCA GAGGTGTCAG TGGCAGGTGG AGGCTAATGG TGCTTTATCC
    CCGGCACTCA CCCCAAGCCC TTCGCCGCTG CCCCTTACTA TAGAAGAAGA CCAAGAATTT
    ACGTATCCAT CTGACATCTT GTTGCCACCA GTTGGGAACT ATTTTGAACT GCCAAGAATC
    AGACTGCCTC CTGGTATTAT GATCAAGCTC AGGGAAATAT CTGGACGCGC TCGACCACAG
    TTTAGACCAA GCATAAAAGA GGTTATCCAG CCTGATGTGA TGGAAGAAAT GGTTGTATCT
    TGCGTCATTA AACACTTGAA CCTGGTCGAT GCATTGCAGT CTTTAATAAA CTTCCAGTAT
    CGAGAAGAAC ATCTAGAAGA ATATGATTTA TTATGTAAAA TAATGGGGGA AACTTTTAAG
    AAGCTCAATG CAATGGAAAG ACAATTGCAG AGTGTTGCAG AACTGGAACA AAAGTGGCAA
    AGTGAAGTGG AAGATGCTCA GAATGGAAAG CTGGAGAACA ACGCTCCCTT TTTTTACGAC
    TATCATTTTA ATGAGAGCAA GTTAAAGGAA CTTGAACTTT TATGTTCTAT GAAAGAGGTC
    CCTTCTGATG GAAGTGATCC AGAAAACATG GTTTTCTCTC TGCGAGAGAA GTTTTTTCAA
    GAGGTGAACT CCCTGATCCA AAAACCTTCC CACCCAATGG CTAAGACCAA AACATTGGTT
    AAGAGTCTGA TGAATAGAGC AGAGCTCCTG TTACATGTGA CTATTGCAGC ACATTCTGGT
    ATCACGAGAA GCATATCTGG CACTCCAACT GAACCTCAAG CTGGCAAGTC TATCTCAGAA
    GCAAAAGTTA TATCTCAAGC CATGCGCCAG CCTGTTTTCC TCCGCAGCAT GTCTGCCCCT
    TCTGACCTGG AAATGATTGG AAGTAGTGAC TTAGAGTTCA GCAGGTCTAA TCCAAGGAGA
    AGGAACATCA CTAGCCACAG GAGCAGCTCC TTCACTCTGC TTCAGTCTTT AGCTATTGAA
    GACTGCAAGG ATAAACCAAC ATACAGTGTC CTTTTAGGGC AACTGTTTGC CTTCATTGGG
    ACCAATCCCG ACCAAGCAGT ATCCAGTAGC AGCTTTCTAC TTGCAGCCCA AACCAGATGG
    CGTCGTGGTA ATACTCGCAA ACAAGCCCTT GTACACATGA GAGAATTACT GACTGCTGCT
    GTCCGTGTTG GGGGTGTGAC ACATTTGGTT GGACCAGTAA CAATGGTGCT ACAGGGAGGA
    CCAAGAATTG AAGAGCTCAC CTGTGGAGGA ATGGTGGAGC TTGTTCAAGA GGCATTTGGA
    GAGACCATGA CCTCAGTGGT GTCACTTTGT GCACGGTATC CCATTGCCTG TGCAAATAGC
    ATTGGGCTTT TGTGTACTAT ACCTTACACA AGGAGTGAAG AAAAATGTTT AGTTCGCAGT
    GGGCTTGTTC AACTGATGGA CCGACTCTGC AGCTTAAGCA ACCAGAGAGA GTCCAGCTCA
    AATGAAAAGC AAACCAAGAA ACAGAAAGTA GCAACTATGG CATGGGCAGC CTTCCAAGTA
    CTGGCCAATA GATGTGTAGA ATGGGAGAAA GAAGAAGGTG GTTCTACTGA GGCGGTACAC
    TCTGGTTTGG CTAGACAAGT CTCCACCCTC CTCACTAACC ATCTAGCAAG GGCTACAGAG
    TGCTGTGGAA ACCAAGCTGC AGGGAATGAT GCCCTACAGG ACGTTCTGAG CCTTCTCAAT
    GATCTCTCTA GGAGTCAGAT AGGGAAGGCA ATTCTGAGCC AGCCTGCTTG TGTGTCCAAA
    CTTCTTTCGT TGCTGCTGGA CCAGCGTCCT TCTCCAAAGC TTGTGCTCAT AATTCTGCAG
    CTGTGCCGCG CTGCACTTCC TCTCATGAGT GTTGAAGACT GTGGAAATGT AGAACTTCCT
    CCATGGAGTT ACTCTTTACC AGCTCTGGAC AGTGAACAGG GGGAGGATCC AAGTGACCCA
    GCCTCCAAAA TAGCATCTTT GTTGCTAGCA AAGTTGGCTG ATTATGTTGT TCCAGGATGT
    CAGACAGTTC TTTCTCCTAC TGCTTCTGAA CCAGACAGCT CATTGTCCAA AAACAGCCCA
    AAAAATTCCC AGAAAGCAGA TAAGGATGCA GAAGAAGAGA GTGAAGCAGT AGATGGCAAG
    CTCTCCATTT TTATTCATAA GCGAGAAGAC CAGTCATCCC ATGAGGTTCT TCAGCCTTTG
    CTTAGTAGTT CAGAGGGAAG GCCTTTTCGT CTTGGTACTG GAGCGAATAT GGAAAAAGTA
    GTAAAGATGG ATCGTGACAT GACTAAGAGT GGATGTTGTG AAGTAATCAC AGAGGAGGCT
    GCAGCTGCTC TCCGCAAAGC CACTAAATGG GCCCAGTCGG GTTTAATTGT TAGCATTGGG
    CCACCTGTGG AGACAGTTAG CCCTGATTCA GCGAGTGGAC TTTCCACTGG TGACAAAAAG
    AAAACAGCCC AGACCTCGAT CTGTAGGGAA AGGAATTCTG AACTTGCCAG GACTGATCCT
    GTACGCCCTT TCATTAGTGG CCATGTAGCA AACAGCATGG CTGCAGAAGT AATTGCTCTG
    CTTCACAGCC TGCTGATGGC ACCAGAGTCA AATGCTGCCC AAATCTGGAC AACAACAGCA
    GAGAAGGTTC TTTCTCGTGC CCTGATGTAT ATCCCACAGC TTGGCAAATA TGCAGAAAGC
    ATCCTAGAGA ACGGCAGCGG TAATGGCAGG AAGCTAGCAA AGCTACAAGG CATTGCAAGG
    CAAGCAGTTG CTGCATTATG TGCGCTTGGT GGATTTAAAG AAACCATTAA AATAGGTTCT
    GAAGTCCAGG TGTTGGGTAA AGGAATATCT GGGAGCATTG GAGTGGTTGT TTCTATTAAT
    GAACAAGAAG GTATTGCAAC AGTGAAATTT CCAGACATAG ATTTTAGAAA ATCATGCAAA
    GCATCTGACA CACTGACAGT ACCATTATTT AGGCTCTGCG TTCCACGGTC AGAGGCATTG
    CCTCTCCATA AACTGTCCAT TACTGAGAAG GTAGTGCAGG CAGTCCAGTC CATGTTACTT
    CCCCAGGAGG GCTGCCTATC TATTCATACC TCGCTTCCTG CAACAGGAGA TGCCTCAACT
    CCAGTAATGG CAGTGGTTCG ACTTCTTGCA GAGATCAGGA CAAGAGCTTG CTTGGTGATG
    GCTCAGCTGT TGGAGGACAG CTCATTTTGT GAAGAATTCA TACAGCAATG TCCAGCTGCT
    GTAGAAGTTC TAAACCTAGT GGCCCAGGAA TGCAGTCCTG GGGAGCGTCT CTCAGTGGTG
    GAAGTTCAGT GTGAAAGACT ACGAATGCTT TATCGGGACT GTGCTCGCCC CCCGCCACCA
    CCTTTGCAGG CTGAGCGCAG ACAGCCCAAG GAAATCACCT GGTCTCCTTC CAGGGTATTC
    CCTCCTGTGA GAGCCTGTAT GTTTTCATCC CATCTAACAG CAGTAACATT TCTGGCTGAC
    CCAAGTGCTG GTGGGGGACT TCCTCGCGGC ACATTTATAT ATGCAACATC CCCTGTTCCA
    GTACAGGCAC CATCTTTTTA CTGGGAAATA GAAGTAGTTT CCTATGGAGA CACAGATGAT
    GACACTGGCC CTATTGTATC ATTTGGGTTT GCTACAGAAG CAGAGAAGCG GGATGGAGCA
    TGGACAAACC CAGTCGGTAC CTGTCTTTTT CATAACAATG GTCGAGCTGT ACATTACAAT
    GGATCAAGTC TCTTACAGTG GAAGAGTGTT CGATTAGATG TGACTCTTGC TCCTGGTGAT
    GTTGCTGGAA TAGGTTGGGA GAGAACTGAA GGAACACCAC CACCCCCAGG GCAGCCTGCC
    AAGGGCAGAG TCTACTTTAC TTACTGTGGA CAGAGGCTTA CTCCGTTCTT AGAGGATGTC
    TCTGGGGGCA TGTGGCCAGT TGTGCATATT CAGAAAAAGA ACACTAAAGT CCGTGCAAAT
    TTTGGATGCA GACAGTTTGC TTTTGCCGAG GGTCAAGCTC ATCGCAATGC AGCAGATTTG
    TGCATTGATT TGGCTGAAGA GATAAGTGCA AACTTTGAAG CATTGCCCTT TGCTATGGCA
    TCTGACAGTG ATAATGATGC TGGGACAAGC ATAGCATCTG ATCCTGGAAC TCATGGACCT
    CCTTGCCGTA TTGCTGCAGT TTCTACTGTG CAACAGCAAT ATGACAGTGA TGCCTCATGC
    CGTTACAAGG TAGAGCTGAG TTATCAAGAC TTTGTGACCT CTGGACCTGA TCCACACCCA
    CCTCCCATTG CAGATGATGA AAGTGATGAT GATGACGATG ATGATATTCC TCGAGAGGAT
    CATTATGCTC TTTTGGTAAA AGCATGGGAG ACCAAAGTTT TTCCAACCAT TCGAAGACGT
    TTCCGTAACG AGGCAGAGAG AAAGTCTGGC CTGGACCAGA TTAAGGGAGC TCTTCAGCTA
    GGAATGGTGG ATATAGCGAG GCAAACTGTA GAGTTCCTCT ATGAAGAAAA TGGTGGCATT
    CCAAGAGATC TCTATCTTCC TACTATTGAG GATATCAAGG ATGAGGCAAA TAAATTCACC
    ATTGATAAAG TTCGCAAAGG ACTCACAGTG GTCATTCGAT CCCCAGATAG CAACAATGTA
    GCTGGGACAA CTGTGGGAAC AGCTTTACCC AAGTTTGCCA TCCGTGGTAT GCTGAAGACA
    TTCTCTCTAC ATGGGGTGGT TCTGGATGTG GATTCAGTTA ATGAGCTGGT TCAGGTAGAG
    ACTTACCTCC GGAGTGAAGG GGTATTAGTG AGATACTGGT ATCCCATAGA TATGCTAGAA
    AGACCTCCTG CGGGATACAG GAGAATGGCA ACCAATGGAC TGGTGACTTT AGATAGCACT
    AACATCCAGA TTCATAGAGA GCTGTTGCGG TGTGAGTCTG CACTTGCCAG GCTTTACTGT
    CGTGTGGCTT TGCTTAACAT CTTTGCTCCT AAGTCTCCGC ACACTTTCAC TCGCCTGTTC
    CATATTCCTG CTATCCGAGA CATTACATTG GAGCATTTAC AGCTTCTGTC AAATCAGCTT
    CTAATGCCAC CCCTTCCAGA CGGAACCATT ACCTCCAGCT CTATCCTTCT TGCCCAGTCC
    TTGCAACATT GCATCTACTC CCAGAACTGC TCAACAACAG ATTTGTTCTA CCAGGGCAAT
    GCACAGCCAA TAAGAGAGTG GCTTGGTGTG GCCATAACCA GGGCCCTGCA TCAAGGAGAG
    GACAGTCTCT TGGATCTGAC AAAACAAATC TGTTCCTTCT TGCAAAATGC GCCGGAACAG
    TTTCCATCTG AAGAATTTCC CATCTCTGAA TCCAAGCTCA GTATGGATGT TAATTTTCCT
    GGGGCAGCTT TTGTTATTGT TTCTTGTATA GAGAGTCCGT CTGGATTTAG AAAAGACTCC
    TCCTTGTACA AGGCTCCCTG GTCTAGAGTC ATGGTATATG GCCTTGGACA TAAAGTAAAG
    AGGAATGGAC AGCTGAACCT GATAGAATCA GTTTGTTATC CACGTGATGC CTCCCCAAGT
    AACACAGGCC TCACTCCCCC ACCAACCACA AACCAGTACC CAACAGCGAT TATTCCCACT
    GACAAGGTCC ACGTCAAACT TGGTGTATCA CCTACTCCTG GTGCTGTGCT GGTCATCCAT
    TCCCTGCCTC TAGAGTTTCC GTTGGCAATG GCATTCACAG AACAGCTGTT AACCTGGAAG
    TTGGAGGACA CAGAAGAGAA GTCAGAAGAT GAGCTTGATA CTGTTCCATC CTCTGCTCTG
    TTGCAAGTGG TAGAACTTTT AGGTACTTTG ATTTGGACAA CAGACCTTGC TGCAGTAATT
    AAGGAGCTTC TGTTCCATCT GTTGGCTGAA CTATTAAGGA AAGTTCACCA TCTAGAGCAG
    AAGAAGAACC CAAGTGGATT ATCTTCCTCG ATAGCCCTGC AGTTGAATCC ATGTCTAGCA
    ATGCTTATGG CTTTACAGAC AGAACTGCGC AAGCTGTATG ATGAAGAAAC TCAAAACTGG
    GCATCTGGGA ATGCATGTGG GGGGTCTGTA CCTGGAGCCT CTGAGCAGGG AAGATTTTCA
    ACTTACTTTC ATGCCCTCAT GGAAGTGTGC CTGGCAGTGG CAGAAGTGAC GCTCCCAATC
    AACATGAGCT CAGCAATCAG TGTGATGGCA TCTACAAATG CCACTAATCT GAGTGACTCT
    TCTTCTTCCT CTTCCTCTTC CCCTGGACAG ACTCCTCAAA GTCCAAGTCT GTTATCAAAG
    AGAAAGAAAG TTAAGATCAA ACGTGAGAAG ACTGCATCTG GAAAACGTAC ACCTTCACGT
    GGTGCAGAGA GTGATGCTGC TTTGCTTAGC ATTGGAGGAA GCAAACCTGA GGACATGTTG
    TGGTTCCATA GGGCATTGAC TTTATTGATG ATCCTAAGGC ATCTAACTAA CAAGGATCCA
    CAGGGGATGG GTGTCACAAA CGATGCTATT GCTGATGCTA GCCAGGCTTT AGTTGGTCCC
    ACAGCTCACA GTCGTCTCCT TGTGATTTCT GGCATCCCTA CACACCTAGA AGAATCAGTG
    GTCAGAAGTG CAATTAGAAA GGCATGTAAT GCTCATGGTG GAGTCTTTAA AGATGAAATC
    TACATTCCAT TGCAGGAAAC TGAAACCCCT AAACCCAAAG ACAGAATAGA AGGGGCAGAA
    TGCAAGACAG AAATAGAAAA ACCCTCTACG CAGCATACAA CCGACAATAT GGATATCAGC
    AGCTCGAGCA GTGTAACTCC TGCAGCAAGT GTCACGGCAT CAGCTTCTAC AAGCCAAGCT
    TCATTGTGCA GCTCCCAAGG GGGGGTATCA CGGACCACAA GTGAAACATC TATGGAGCAG
    TTTGCCCCAG AACATGCTGT GCCATCAGGG CTTCTGGAAC CAACTATTAT CTCCAGCCAG
    GAGAGTCTGG ATCTTTCTAT GTGCAGCACA GGAAGCCTCG GCAGTCTGGG AAGTCTTGTT
    GAACAGCTTG ATAATGTTGA AAACTCTTTG TCTGACGTGG GCTCCCTATA CACAGCTACA
    TCACTAGATA GCCATATGGC AGAGTCGCGG CCAATCAAAG GATTTGCAGT TGTAGAGGTG
    CGATCACGAG CTAAAATAGA GAAAATCCGT GCCACTTTAT TTAACAGTAG TGACCTCATT
    GGGTTATCTG GTTTGGATGG TGAAGATGAG CTGATGGAAA TGTCTACTGA GGAGATCTTG
    ACTGTTTCAA CAGTGAATCA GAGCTTGTTT GATACCCAAG GAATACCCGG TCTGGCTGAT
    TATTTCATGG ATAAATCAAT GAAAGGTGAT AAATTAGTCC CGGAAGCTCG TGATGTTCTG
    ACTGAGATTT TCAAGAGCTG CATTCACGCC GAGCAGATGC TGAGTCTGAC TCCAGCCAAG
    TTTATCAAAG TGTCTGATAT CTACCTTAGC AAGGAGCAAA TCAATTCACA GACCCCAGGG
    AACTTACTGC ATACCTTTTT CACCAATGTT CGACCACCTA AAAAAGTCCT GGAAGATCAA
    CTAACGCAGA TCTTAAGAAA ATATGGAATT CCCAAGCCTA AATTTGACAA AAGCAAATAC
    AGCAAAACTG GGAAGGAGCA ACATCCTGTG AAAGTTGTAA GCACCAAAAG ACCTGTGGGC
    AAACCACCGA CCAAAGACAA GGCTGTATTC AACAGCGTCA GGACTGCTTT AAGTGAGAAA
    AAACCAACTG TGAAGCCAAA ATCTCCGGAG AAGGGCAGGC CAGATGAGAA GGACCCAGAC
    AAATCTCCAA CAAAGAAGCA AGAAAGGTTT CACTTGTGTC CCTATCTAGT TCCCGAGGAG
    AGATATCTGA CGTTGGATGG ATTTCACCGC TTTGTAGTAG ATCGGGCTAA GCAAGACATT
    CGAAGCATCT GGAGAGCTGT CTTGGCGTGT GGATATGATC TTCATTTTGA AAGGTGTGCC
    TGCATTGATA CAAGGCATGC TCAGAAATCT AGCAGAAAAT GGACCTTGGA AATGGATGTT
    GCTCTTGTTC AGTATATCAA CCGCCTTTGC CGTCACCTTG CCATAACGCC AGCCCGACTA
    CACCCTCATG AGGTTTATCT GGATCTTGCA GATACGGCTG ACCCAAGAGT CTCCTGCCTG
    CTGAATGTAC CAATAGAAAG CCTGCGCCTC CGATTTGCTC TTCTTCAGTC CCTCAACACT
    ACTTTAGAGA CCTTTTTCCT GCCCTTAGTG GAGCTACGGC AAACAGACGT ATACACACAC
    AGCATCGCAT CCCTACTCCA GCTAGCTAAA GGGTTAATCT TTTACGATAC AAAAGTGAAT
    GTGATGAACC GGGTATTGAA CGCAACAGTG CAGAGGACAG CAGATCATGC AGCCCCTGAG
    ATCACGCTGG ACCCACTAGA AATTGTAGGA GGGGAAATTC GTACCTCAGA AAACTCCTAC
    TTCTGCCAGG CAGCCCGACA GTTGGCATGC ATCCCTTCCT CACAACTGTG TGTCAAACTG
    GCAAGTGGAG GAGACCCCAC GTATGCATTT AACATCCGCT TTACTGGAGA GGAGGTGCAT
    GGTACAAGTG GCTCATTCCG TCATTTCCTC TGGCAAGTGT GTAAAGAACT ACAGAGCTCC
    TCGCTGTCTC TTCTACTGCT CTGCCCCAGC TCTGCAGTTA ATAAGAATAA GGGAAAATAT
    CTTCTAACTC CCAGTCCCAT AACATATGCA GAAGAGCAGT TGCTCCATTT CTTTGGCCAA
    CTACTGGGAA TAGCCATCAG AGCCGATGTT CCACTCCCTC TGGATCTACT CCCCTGCTTC
    TGGAAGATGC TTGTAGGAGA ACCTTTGGAC CCAGAAACTG ATCTTTATGA AGCAGATATT
    CTCACCCACA ACTACATCAA GAAGTTTGAA AATGTAAATG ATGAGTCAGA GCTGGAAGCT
    CTTTGTGCTG AAATTGCCTC TCAGCATCTT CCTACCGAAA GTCCAGATTC TCCTCATAAA
    CCAGCCTGCA AATTTACATA CTTGACAATG ACTGGTGAAG AAGTGGAGCT GTGCCCTAAG
    GGAAGACACA TTTCTGTAGT CTGGGAAAAC AAAGATATAT ATGCAATGGG AATCCGGAAC
    CTTCGTATAC GTGAGCTGAA GAACCAGGAG TGTATGAGCG CAGTAAGGGC TGGGCTGGGC
    TCCATTATAC CTCTGCAGCT CCTCACCACT CTAAATGCTC TAGAGATGGA ACTAAGGACA
    TGTGGCCTAC CATATATTAA TTTGGAATTC TTAAAGGCTC ACACCATGTA TCAAGTGGGT
    CTAATGGAGA CAGATCAACA CATTGAGTAT TTTTGGTCTG CCTTAGAAAT GTTCACTCAG
    GAAGAACTTT GCAAATTCAT AAAATTCGCC TGCAATCAGG AACGCATTCC CTTTACATGT
    CCATGCAAAG ATGGTGGGCC AGACACGGCT CATGTTCCAC CTTATCCTAT GAAAATAGCA
    CCACCCGATG GAGCAACAGG CTCCCCAGAT TCCAGGTACA TCCGAGTGGA GACATGTATG
    TTCATGATCA AGCTTCCGCA GTACTCGTCT CTAGAGATGA TGCTGGAGAA ACTGAGATAT
    GCCATTCACT ACAGGGAAGA CCCACTCAGC GGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012824248.1
    CDS323..13558
    Translation

    Target ORF information:

    RefSeq Version XM_012968794.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 4 (hectd4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012968794.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGGGGTCGG CGGCTGCCGG GGACTCGGCT CAGTGGCTGT CGGTGAAAGA AGAGACTATC 
    TTTTTACATG ATGGGCTGAT CCGGGTCACA GACCTGGCGG AGTTGCCCAG CGAAATCGGG
    GTCAGTGAGG CCGGGGACGC AGAACTGGAG ATATTGACTT TTGAGACTAA GAACCCTATT
    GAGCTAGCAG AGCATTTACG TGCAGTGTGT GGGAACCAGA GCAATGCCTA TGCACGATTG
    CTGGAATACC GCTTGAATGC GTTACGTGGG TTATGGAATG CCCAACGCCA GCTGGCGCTT
    GAGGATCAAC ATGAAAGAGA TGGGGCTGAT GACGAAACCT TGGCCCTGCT TAAACGGCAG
    GGTTTGTTGC AACAGCCGGA ACAGGCTCCA TTTACATCTC GAATGGGGCT TTTACTGGTG
    TTCCCCCTTA TCCAGTCCCA AAGTAAAACA GACCCTTCAC TTTGTAACTT GACAGCTGAG
    GTGCTTTTAA ATTGCCTTCG GGACTGCCAA CCCCTGAGCT TGACTAAAGA ACCAGCTGAC
    TGCCTGAATG GAATAGAGTC CTTACTGTGT TCTTGGTTAG AAGACACTCC AAATGTGGGC
    CAACAAGTCT CCTTTAAGCA AAAGGAAAAT TCTGCTGCTG CTTTGGTTGC CTTAGCTTGT
    GCTAGGGGAT CATTAAAAAC ATTTATTCAC ACTGTACACT TGCTGCAAAA ACAGACTGAC
    CTAGGATCCC TTCCTGTGGC AGATGTATTG TATAGGTTGC TGCTTTTAGA GGGAGGTCCA
    GGTTCTCCCT CTTGTTTACT GGGAAGCAAA CACATTGTAT CCTGGGGTTT TGAGGATATG
    CTTCCAGCTC CTGATGGGAT CAGTGGTTCA AACAATGAGA ACAAAGATGC AGATTTGGGT
    CGCTGTGTAG CATGCGATGG GCTTTATCTT TACACAACTA ACTCTGTAGG TAGAGGATTG
    AGCAAAGTAG GGTCTGGGCT GCATGGCACT TTACGAGGTT TTGTTTATTC CCGAAACGAG
    GAGCTAGAGG CTGGCTGGAT TGCTTATGGA AGTGGGAAGA TACTTCACAG GCCTGCCTCC
    TTTGATAACA AACCTCAATA TCTTTTCCAA TTAATTGACC AATACACACT TCAGGTCTCT
    TATATAGTCA GCATGCCAGC CAATCATTTC CCGGTTGGCA GCAATATGAC TACGGTGCAC
    CTCTCCTCGG ATGGCACGTA TTTTTACTGG ATTTGGTCTC CAGCTAGTTT AAATGAGAAA
    ACCCCCAAAG GACACTCTGT CTTCATGGAT ATATTTGAGT TAAATGTGCA AAATGGAGTA
    TGCATTGCAA GCCCTCTTCA TGAGCGCATT ATATTAATGA GGAAAGAGGG AGAATCTGCT
    AAAAGTATTA ATGAAATGCT TTTGAGCAGA TTATCACGAT ACAGAGCTTC TCCTTCAGCA
    ACTCTAGCAG CACTTACAGG ATCTACAATC TCTAACACTC TAAAAGAGGA CCAGACTGCT
    GCAAATACGA GCTGCGGGCT TCCTTTGAAA ATGTTAAGAA AGACTCCAAT GTATACTTGT
    GGCACATACT TAGTACTACT CGTGCCACCT CCGGGTGGGT CAGGAAGTTC AGCAACAAGA
    TCTTTGTTTG GTGGAACCAG CGGCCTCTCC TCTTTAAAAA TTCTGGCTTC CAGCTTGGTA
    TTTAACATTT CTGATGGGCA GTTCACCAAC AGAGCAGATC TTATTGATGC ATCTGGAAGT
    TCTCTTGGAA GGGGTGCTTT GGTGACAGGC CTAGGTGCCT GCTATGATAC AATGAATAAC
    ATGATCTGGA CGTGCTCCAA TGATTACATC GATCAGTGGT GTAATCCTGG AAACCAGGCT
    TTCCATGTGG TGTGTCAGAG GCTTGGTGTC ACTCACATCA TTGCTGATCC CAAAGGAGAG
    GCAGTATCAA CAAATGAAGT TATAAACCAG TTGCTTCACC ATGTGGGTGC TATGTGTATT
    CACCAACTCA ATCTCCTGGC TAATAGCAAC AGTTCTCCAA TCAGTACCTT TCTGGGCAAA
    CAACATCCTA TAGAGCCACA TCATCTTAAT AGTATATGTG ACATCATGGA GAAAGCCATG
    GTCAATGGTG ATAAATGCAT TATTCGCTGC ATCTTGGTTG TTTTCCAGGT GGTATTTAAA
    TTCTTTCTAA ACCCACAAAC TGAGAGGAAT CGAGAAACCA TAAGGAGGGC TGGTTTGCTC
    CTTTGGCAGT TGCTCATGGC GCCTAAGGAC CAAATTTGTC CTGAAATACA GAGAGAGGTC
    TGCTTGGCTA TTAGCTTTGG TTTAAACATT TTGAATCCAG GAGAGCTAGA AATTAATAAC
    TTGCTCAAGC TGATTTTAAC TGAAGGGGAC AGAAATTGTG GCCTCTCCCA ACTCCGTGAT
    GTCATTTTGA CAAACCTCAC TGAACAGCTT CAGAATAACA GGTTTGGAAG TGAAGATGAT
    GAACATTACA GGCTTAATGA TGAACTATTG CACTATATTT TGAAGATTGT TGTTCGTGAA
    TCCTGTGTTC TGATCACCAA GTGCCAAACA GTGTCTAAAG AAGAATTCCA AAGACTCTTG
    TCAACAGTGC CTGTGGCTTC TCCCTGTCTA TGCTATTTAA TGGCTGTACA GAATCATCTC
    CTGAGTAACA CTGTCTTAAT TAAACCTGAT GACAGCGATG ACAGTGACAA CTCCCTGCAG
    GGGGAGACAT TGAAGGAACT CCAGGCCAGC ATCTTGGCTT TGGCAACCCA GATACTTTCT
    GGTTGTGATG AAGTTCTGGA TATTCTGCAG CGAGTGACCA CTGCTCTTAT CAACAGCGAC
    CTGTTAGACA GAGATTTAAG ACTGAAAGGC CTTGAACAGA TCACAAAGTC CACCCTGCTT
    GGCCATCTCC TCCCAGTACT TCTCACATCC CTTATGCATC CAAACTTACA GACATTAACC
    ATGGCAGATG CGCTTATGCC TCAGCTGGTG CAGCTTGTTC TTTACACTAG CCAGACTGCA
    CTGCTGCTTA AAACCCAGTG TCCAGTATTG GCAGATACAG GGGACACCAC CAGTGGAATT
    CCAGAGCAAA AAAACAAACT GTTAACTGAT GACAGGATGC TGGAAGAAAA GGAGGAGCCT
    GGTTTCCTCA CTGGGCTAAA GATTCCTGCG CCATGGGCTG CAGGAAAGAC TGTGGAAACA
    TCTCATCCAG TCAGAGACAA TTACAAGTTT AAAGAGACTG TGCACATTCC TGGAGCTCGT
    TGTTTGTACC TCCGGTTTGA CAGCAGGTGC TCTTCACAGT ACGATTATGA TAAGCTGGTG
    ATATATGCGG GACCCAACAC AAACAGCAGA AAAGTTGCCG AATATGGTGG AAATACCCTG
    GGGTATGGCA GTAGAAGTGT TTTAGGTACA GGCTGGCCAA AAGATTTGGT TAAGGTGGAA
    GGGGATACTG TGACATTCTC CTTCGAAATG AGAAGTGGGC GTGAGCACAA CACTCCTGAT
    AAAGCAATGT GGGGTTTTGC GTGCACTGTC AGAGCTCAGG AGTCCTCAGA AGATGTGTCA
    GGGGGCTTGC CATTTCTAGT AGACCTGGCT TTAGGTCTGT CTGTCTTGGC TTGTTCCATG
    CTGCGTATAT TGTACAATGG TCCAGAAATC ACAAAAGAAG AGTTGACTTG TCACGATCTG
    TTGAGGTCAA AGCTTTTGCA GAGGTGTCAG TGGCAGGTGG AGGCTAATGG TGCTTTATCC
    CCGGCACTCA CCCCAAGCCC TTCGCCGCTG CCCCTTACTA TAGAAGAAGA CCAAGAATTT
    ACGTATCCAT CTGACATCTT GTTGCCACCA GTTGGGAACT ATTTTGAACT GCCAAGAATC
    AGACTGCCTC CTGGTATTAT GATCAAGCTC AGGGAAATAT CTGGACGCGC TCGACCACAG
    TTTAGACCAA GCATAAAAGA GGTTATCCAG CCTGATGTGA TGGAAGAAAT GGTTGTATCT
    TGCGTCATTA AACACTTGAA CCTGGTCGAT GCATTGCAGT CTTTAATAAA CTTCCAGTAT
    CGAGAAGAAC ATCTAGAAGA ATATGATTTA TTATGTAAAA TAATGGGGGA AACTTTTAAG
    AAGCTCAATG CAATGGAAAG ACAATTGCAG AGTGTTGCAG AACTGGAACA AAAGTGGCAA
    AGTGAAGTGG AAGATGCTCA GAATGGAAAG CTGGAGAACA ACGCTCCCTT TTTTTACGAC
    TATCATTTTA ATGAGAGCAA GTTAAAGGAA CTTGAACTTT TATGTTCTAT GAAAGAGGTC
    CCTTCTGATG GAAGTGATCC AGAAAACATG GTTTTCTCTC TGCGAGAGAA GTTTTTTCAA
    GAGGTGAACT CCCTGATCCA AAAACCTTCC CACCCAATGG CTAAGACCAA AACATTGGTT
    AAGAGTCTGA TGAATAGAGC AGAGCTCCTG TTACATGTGA CTATTGCAGC ACATTCTGGT
    ATCACGAGAA GCATATCTGG CACTCCAACT GAACCTCAAG CTGGCAAGTC TATCTCAGAA
    GCAAAAGTTA TATCTCAAGC CATGCGCCAG CCTGTTTTCC TCCGCAGCAT GTCTGCCCCT
    TCTGACCTGG AAATGATTGG AAGTAGTGAC TTAGAGTTCA GCAGGTCTAA TCCAAGGAGA
    AGGAACATCA CTAGCCACAG GAGCAGCTCC TTCACTCTGC TTCAGTCTTT AGCTATTGAA
    GACTGCAAGG ATAAACCAAC ATACAGTGTC CTTTTAGGGC AACTGTTTGC CTTCATTGGG
    ACCAATCCCG ACCAAGCAGT ATCCAGTAGC AGCTTTCTAC TTGCAGCCCA AACCAGATGG
    CGTCGTGGTA ATACTCGCAA ACAAGCCCTT GTACACATGA GAGAATTACT GACTGCTGCT
    GTCCGTGTTG GGGGTGTGAC ACATTTGGTT GGACCAGTAA CAATGGTGCT ACAGGGAGGA
    CCAAGAATTG AAGAGCTCAC CTGTGGAGGA ATGGTGGAGC TTGTTCAAGA GGCATTTGGA
    GAGACCATGA CCTCAGTGGT GTCACTTTGT GCACGGTATC CCATTGCCTG TGCAAATAGC
    ATTGGGCTTT TGTGTACTAT ACCTTACACA AGGAGTGAAG AAAAATGTTT AGTTCGCAGT
    GGGCTTGTTC AACTGATGGA CCGACTCTGC AGCTTAAGCA ACCAGAGAGA GTCCAGCTCA
    AATGAAAAGC AAACCAAGAA ACAGAAAGTA GCAACTATGG CATGGGCAGC CTTCCAAGTA
    CTGGCCAATA GATGTGTAGA ATGGGAGAAA GAAGAAGGTG GTTCTACTGA GGCGGTACAC
    TCTGGTTTGG CTAGACAAGT CTCCACCCTC CTCACTAACC ATCTAGCAAG GGCTACAGAG
    TGCTGTGGAA ACCAAGCTGC AGGGAATGAT GCCCTACAGG ACGTTCTGAG CCTTCTCAAT
    GATCTCTCTA GGAGTCAGAT AGGGAAGGCA ATTCTGAGCC AGCCTGCTTG TGTGTCCAAA
    CTTCTTTCGT TGCTGCTGGA CCAGCGTCCT TCTCCAAAGC TTGTGCTCAT AATTCTGCAG
    CTGTGCCGCG CTGCACTTCC TCTCATGAGT GTTGAAGACT GTGGAAATGT AGAACTTCCT
    CCATGGAGTT ACTCTTTACC AGCTCTGGAC AGTGAACAGG GGGAGGATCC AAGTGACCCA
    GCCTCCAAAA TAGCATCTTT GTTGCTAGCA AAGTTGGCTG ATTATGTTGT TCCAGGATGT
    CAGACAGTTC TTTCTCCTAC TGCTTCTGAA CCAGACAGCT CATTGTCCAA AAACAGCCCA
    AAAAATTCCC AGAAAGCAGA TAAGGATGCA GAAGAAGAGA GTGAAGCAGT AGATGGCAAG
    CTCTCCATTT TTATTCATAA GCGAGAAGAC CAGTCATCCC ATGAGGTTCT TCAGCCTTTG
    CTTAGTAGTT CAGAGGGAAG GCCTTTTCGT CTTGGTACTG GAGCGAATAT GGAAAAAGTA
    GTAAAGATGG ATCGTGACAT GACTAAGAGT GGATGTTGTG AAGTAATCAC AGAGGAGGCT
    GCAGCTGCTC TCCGCAAAGC CACTAAATGG GCCCAGTCGG GTTTAATTGT TAGCATTGGG
    CCACCTGTGG AGACAGTTAG CCCTGATTCA GCGAGTGGAC TTTCCACTGG TGACAAAAAG
    AAAACAGCCC AGACCTCGAT CTGTAGGGAA AGGAATTCTG AACTTGCCAG GACTGATCCT
    GTACGCCCTT TCATTAGTGG CCATGTAGCA AACAGCATGG CTGCAGAAGT AATTGCTCTG
    CTTCACAGCC TGCTGATGGC ACCAGAGTCA AATGCTGCCC AAATCTGGAC AACAACAGCA
    GAGAAGGTTC TTTCTCGTGC CCTGATGTAT ATCCCACAGC TTGGCAAATA TGCAGAAAGC
    ATCCTAGAGA ACGGCAGCGG TAATGGCAGG AAGCTAGCAA AGCTACAAGG CATTGCAAGG
    CAAGCAGTTG CTGCATTATG TGCGCTTGGT GGATTTAAAG AAACCATTAA AATAGGTTCT
    GAAGTCCAGG TGTTGGGTAA AGGAATATCT GGGAGCATTG GAGTGGTTGT TTCTATTAAT
    GAACAAGAAG GTATTGCAAC AGTGAAATTT CCAGACATAG ATTTTAGAAA ATCATGCAAA
    GCATCTGACA CACTGACAGT ACCATTATTT AGGCTCTGCG TTCCACGGTC AGAGGCATTG
    CCTCTCCATA AACTGTCCAT TACTGAGAAG GTAGTGCAGG CAGTCCAGTC CATGTTACTT
    CCCCAGGAGG GCTGCCTATC TATTCATACC TCGCTTCCTG CAACAGGAGA TGCCTCAACT
    CCAGTAATGG CAGTGGTTCG ACTTCTTGCA GAGATCAGGA CAAGAGCTTG CTTGGTGATG
    GCTCAGCTGT TGGAGGACAG CTCATTTTGT GAAGAATTCA TACAGCAATG TCCAGCTGCT
    GTAGAAGTTC TAAACCTAGT GGCCCAGGAA TGCAGTCCTG GGGAGCGTCT CTCAGTGGTG
    GAAGTTCAGT GTGAAAGACT ACGAATGCTT TATCGGGACT GTGCTCGCCC CCCGCCACCA
    CCTTTGCAGG CTGAGCGCAG ACAGCCCAAG GAAATCACCT GGTCTCCTTC CAGGGTATTC
    CCTCCTGTGA GAGCCTGTAT GTTTTCATCC CATCTAACAG CAGTAACATT TCTGGCTGAC
    CCAAGTGCTG GTGGGGGACT TCCTCGCGGC ACATTTATAT ATGCAACATC CCCTGTTCCA
    GTACAGGCAC CATCTTTTTA CTGGGAAATA GAAGTAGTTT CCTATGGAGA CACAGATGAT
    GACACTGGCC CTATTGTATC ATTTGGGTTT GCTACAGAAG CAGAGAAGCG GGATGGAGCA
    TGGACAAACC CAGTCGGTAC CTGTCTTTTT CATAACAATG GTCGAGCTGT ACATTACAAT
    GGATCAAGTC TCTTACAGTG GAAGAGTGTT CGATTAGATG TGACTCTTGC TCCTGGTGAT
    GTTGCTGGAA TAGGTTGGGA GAGAACTGAA GGAACACCAC CACCCCCAGG GCAGCCTGCC
    AAGGGCAGAG TCTACTTTAC TTACTGTGGA CAGAGGCTTA CTCCGTTCTT AGAGGATGTC
    TCTGGGGGCA TGTGGCCAGT TGTGCATATT CAGAAAAAGA ACACTAAAGT CCGTGCAAAT
    TTTGGATGCA GACAGTTTGC TTTTGCCGAG GGTCAAGCTC ATCGCAATGC AGCAGATTTG
    TGCATTGATT TGGCTGAAGA GATAAGTGCA AACTTTGAAG CATTGCCCTT TGCTATGGCA
    TCTGACAGTG ATAATGATGC TGGGACAAGC ATAGCATCTG ATCCTGGAAC TCATGGACCT
    CCTTGCCGTA TTGCTGCAGT TTCTACTGTG CAACAGCAAT ATGACAGTGA TGCCTCATGC
    CGTTACAAGG TAGAGCTGAG TTATCAAGAC TTTGTGACCT CTGGACCTGA TCCACACCCA
    CCTCCCATTG CAGATGATGA AAGTGATGAT GATGACGATG ATGATATTCC TCGAGAGGAT
    CATTATGCTC TTTTGGTAAA AGCATGGGAG ACCAAAGTTT TTCCAACCAT TCGAAGACGT
    TTCCGTAACG AGGCAGAGAG AAAGTCTGGC CTGGACCAGA TTAAGGGAGC TCTTCAGCTA
    GGAATGGTGG ATATAGCGAG GCAAACTGTA GAGTTCCTCT ATGAAGAAAA TGGTGGCATT
    CCAAGAGATC TCTATCTTCC TACTATTGAG GATATCAAGG ATGAGGCAAA TAAATTCACC
    ATTGATAAAG TTCGCAAAGG ACTCACAGTG GTCATTCGAT CCCCAGATAG CAACAATGTA
    GCTGGGACAA CTGTGGGAAC AGCTTTACCC AAGTTTGCCA TCCGTGGTAT GCTGAAGACA
    TTCTCTCTAC ATGGGGTGGT TCTGGATGTG GATTCAGTTA ATGAGCTGGT TCAGGTAGAG
    ACTTACCTCC GGAGTGAAGG GGTATTAGTG AGATACTGGT ATCCCATAGA TATGCTAGAA
    AGACCTCCTG CGGGATACAG GAGAATGGCA ACCAATGGAC TGGTGACTTT AGATAGCACT
    AACATCCAGA TTCATAGAGA GCTGTTGCGG TGTGAGTCTG CACTTGCCAG GCTTTACTGT
    CGTGTGGCTT TGCTTAACAT CTTTGCTCCT AAGTCTCCGC ACACTTTCAC TCGCCTGTTC
    CATATTCCTG CTATCCGAGA CATTACATTG GAGCATTTAC AGCTTCTGTC AAATCAGCTT
    CTAATGCCAC CCCTTCCAGA CGGAACCATT ACCTCCAGCT CTATCCTTCT TGCCCAGTCC
    TTGCAACATT GCATCTACTC CCAGAACTGC TCAACAACAG ATTTGTTCTA CCAGGGCAAT
    GCACAGCCAA TAAGAGAGTG GCTTGGTGTG GCCATAACCA GGGCCCTGCA TCAAGGAGAG
    GACAGTCTCT TGGATCTGAC AAAACAAATC TGTTCCTTCT TGCAAAATGC GCCGGAACAG
    TTTCCATCTG AAGAATTTCC CATCTCTGAA TCCAAGCTCA GTATGGATGT TAATTTTCCT
    GGGGCAGCTT TTGTTATTGT TTCTTGTATA GAGAGTCCGT CTGGATTTAG AAAAGACTCC
    TCCTTGTACA AGGCTCCCTG GTCTAGAGTC ATGGTATATG GCCTTGGACA TAAAGTAAAG
    AGGAATGGAC AGCTGAACCT GATAGAATCA GTTTGTTATC CACGTGATGC CTCCCCAAGT
    AACACAGGCC TCACTCCCCC ACCAACCACA AACCAGTACC CAACAGCGAT TATTCCCACT
    GACAAGGTCC ACGTCAAACT TGGTGTATCA CCTACTCCTG GTGCTGTGCT GGTCATCCAT
    TCCCTGCCTC TAGAGTTTCC GTTGGCAATG GCATTCACAG AACAGCTGTT AACCTGGAAG
    TTGGAGGACA CAGAAGAGAA GTCAGAAGAT GAGCTTGATA CTGTTCCATC CTCTGCTCTG
    TTGCAAGTGG TAGAACTTTT AGGTACTTTG ATTTGGACAA CAGACCTTGC TGCAGTAATT
    AAGGAGCTTC TGTTCCATCT GTTGGCTGAA CTATTAAGGA AAGTTCACCA TCTAGAGCAG
    AAGAAGAACC CAAGTGGATT ATCTTCCTCG ATAGCCCTGC AGTTGAATCC ATGTCTAGCA
    ATGCTTATGG CTTTACAGAC AGAACTGCGC AAGCTGTATG ATGAAGAAAC TCAAAACTGG
    GCATCTGGGA ATGCATGTGG GGGGTCTGTA CCTGGAGCCT CTGAGCAGGG AAGATTTTCA
    ACTTACTTTC ATGCCCTCAT GGAAGTGTGC CTGGCAGTGG CAGAAGTGAC GCTCCCAATC
    AACATGAGCT CAGCAATCAG TGTGATGGCA TCTACAAATG CCACTAATCT GAGTGACTCT
    TCTTCTTCCT CTTCCTCTTC CCCTGGACAG ACTCCTCAAA GTCCAAGTCT GTTATCAAAG
    AGAAAGAAAG TTAAGATCAA ACGTGAGAAG ACTGCATCTG GAAAACGTAC ACCTTCACGT
    GGTGCAGAGA GTGATGCTGC TTTGCTTAGC ATTGGAGGAA GCAAACCTGA GGACATGTTG
    TGGTTCCATA GGGCATTGAC TTTATTGATG ATCCTAAGGC ATCTAACTAA CAAGGATCCA
    CAGGGGATGG GTGTCACAAA CGATGCTATT GCTGATGCTA GCCAGGCTTT AGTTGGTCCC
    ACAGCTCACA GTCGTCTCCT TGTGATTTCT GGCATCCCTA CACACCTAGA AGAATCAGTG
    GTCAGAAGTG CAATTAGAAA GGCATGTAAT GCTCATGGTG GAGTCTTTAA AGATGAAATC
    TACATTCCAT TGCAGGAAAC TGAAACCCCT AAACCCAAAG ACAGAATAGA AGGGGCAGAA
    TGCAAGACAG AAATAGAAAA ACCCTCTACG CAGCATACAA CCGACAATAT GGATATCAGC
    AGCTCGAGCA GTGTAACTCC TGCAGCAAGT GTCACGGCAT CAGCTTCTAC AAGCCAAGCT
    TCATTGTGCA GCTCCCAAGG GGGGGTATCA CGGACCACAA GTGAAACATC TATGGAGCAG
    TTTGCCCCAG AACATGCTGT GCCATCAGGG CTTCTGGAAC CAACTATTAT CTCCAGCCAG
    GAGAGTCTGG ATCTTTCTAT GTGCAGCACA GGAAGCCTCG GCAGTCTGGG AAGTCTTGTT
    GAACAGCTTG ATAATGTTGA AAACTCTTTG TCTGACGTGG GCTCCCTATA CACAGCTACA
    TCACTAGATA GCCATATGGC AGAGTCGCGG CCAATCAAAG GATTTGCAGT TGTAGAGGTG
    CGATCACGAG CTAAAATAGA GAAAATCCGT GCCACTTTAT TTAACAGTAG TGACCTCATT
    GGGTTATCTG GTTTGGATGG TGAAGATGAG CTGATGGAAA TGTCTACTGA GGAGATCTTG
    ACTGTTTCAA CAGTGAATCA GAGCTTGTTT GATACCCAAG GAATACCCGG TCTGGCTGAT
    TATTTCATGG ATAAATCAAT GAAAGGTGAT AAATTAGTCC CGGAAGCTCG TGATGTTCTG
    ACTGAGATTT TCAAGAGCTG CATTCACGCC GAGCAGATGC TGAGTCTGAC TCCAGCCAAG
    TTTATCAAAG TGTCTGATAT CTACCTTAGC AAGGAGCAAA TCAATTCACA GACCCCAGGG
    AACTTACTGC ATACCTTTTT CACCAATGTT CGACCACCTA AAAAAGTCCT GGAAGATCAA
    CTAACGCAGA TCTTAAGAAA ATATGGAATT CCCAAGCCTA AATTTGACAA AAGCAAATAC
    AGCAAAACTG GGAAGGAGCA ACATCCTGTG AAAGTTGTAA GCACCAAAAG ACCTGTGGGC
    AAACCACCGA CCAAAGACAA GGCTGTATTC AACAGCGTCA GGACTGCTTT AAGTGAGAAA
    AAACCAACTG TGAAGCCAAA ATCTCCGGAG AAGGGCAGGC CAGATGAGAA GGACCCAGAC
    AAATCTCCAA CAAAGAAGCA AGAAAGGTTT CACTTGTGTC CCTATCTAGT TCCCGAGGAG
    AGATATCTGA CGTTGGATGG ATTTCACCGC TTTGTAGTAG ATCGGGCTAA GCAAGACATT
    CGAAGCATCT GGAGAGCTGT CTTGGCGTGT GGATATGATC TTCATTTTGA AAGGTGTGCC
    TGCATTGATA CAAGGCATGC TCAGAAATCT AGCAGAAAAT GGACCTTGGA AATGGATGTT
    GCTCTTGTTC AGTATATCAA CCGCCTTTGC CGTCACCTTG CCATAACGCC AGCCCGACTA
    CACCCTCATG AGGTTTATCT GGATCTTGCA GATACGGCTG ACCCAAGAGT CTCCTGCCTG
    CTGAATGTAC CAATAGAAAG CCTGCGCCTC CGATTTGCTC TTCTTCAGTC CCTCAACACT
    ACTTTAGAGA CCTTTTTCCT GCCCTTAGTG GAGCTACGGC AAACAGACGT ATACACACAC
    AGCATCGCAT CCCTACTCCA GCTAGCTAAA GGGTTAATCT TTTACGATAC AAAAGTGAAT
    GTGATGAACC GGGTATTGAA CGCAACAGTG CAGAGGACAG CAGATCATGC AGCCCCTGAG
    ATCACGCTGG ACCCACTAGA AATTGTAGGA GGGGAAATTC GTACCTCAGA AAACTCCTAC
    TTCTGCCAGG CAGCCCGACA GTTGGCATGC ATCCCTTCCT CACAACTGTG TGTCAAACTG
    GCAAGTGGAG GAGACCCCAC GTATGCATTT AACATCCGCT TTACTGGAGA GGAGGTGCAT
    GGTACAAGTG GCTCATTCCG TCATTTCCTC TGGCAAGTGT GTAAAGAACT ACAGAGCTCC
    TCGCTGTCTC TTCTACTGCT CTGCCCCAGC TCTGCAGTTA ATAAGAATAA GGGAAAATAT
    CTTCTAACTC CCAGTCCCAT AACATATGCA GAAGAGCAGT TGCTCCATTT CTTTGGCCAA
    CTACTGGGAA TAGCCATCAG AGCCGATGTT CCACTCCCTC TGGATCTACT CCCCTGCTTC
    TGGAAGATGC TTGTAGGAGA ACCTTTGGAC CCAGAAACTG ATCTTTATGA AGCAGATATT
    CTCACCCACA ACTACATCAA GAAGTTTGAA AATGTAAATG ATGAGTCAGA GCTGGAAGCT
    CTTTGTGCTG AAATTGCCTC TCAGCATCTT CCTACCGAAA GTCCAGATTC TCCTCATAAA
    CCAGCCTGCA AATTTACATA CTTGACAATG ACTGGTGAAG AAGTGGAGCT GTGCCCTAAG
    GGAAGACACA TTTCTGTAGT CTGGGAAAAC AAAGATATAT ATGCAATGGG AATCCGGAAC
    CTTCGTATAC GTGAGCTGAA GAACCAGGAG TGTATGAGCG CAGTAAGGGC TGGGCTGGGC
    TCCATTATAC CTCTGCAGCT CCTCACCACT CTAAATGCTC TAGAGATGGA ACTAAGGACA
    TGTGGCCTAC CATATATTAA TTTGGAATTC TTAAAGGCTC ACACCATGTA TCAAGTGGGT
    CTAATGGAGA CAGATCAACA CATTGAGTAT TTTTGGTCTG CCTTAGAAAT GTTCACTCAG
    GAAGAACTTT GCAAATTCAT AAAATTCGCC TGCAATCAGG AACGCATTCC CTTTACATGT
    CCATGCAAAG ATGGTGGGCC AGACACGGCT CATGTTCCAC CTTATCCTAT GAAAATAGCA
    CCACCCGATG GAGCAACAGG CTCCCCAGAT TCCAGGTACA TCCGAGTGGA GACATGTATG
    TTCATGATCA AGCTTCCGCA GTACTCGTCT CTAGAGATGA TGCTGGAGAA ACTGAGATAT
    GCCATTCACT ACAGGGAAGA CCCACTCAGC GGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31530
    Clone ID Related Accession (Same CDS sequence) XM_012968799.2
    Accession Version XM_012968799.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13218bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product probable E3 ubiquitin-protein ligase HECTD4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968799.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
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    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGGGGTCGG CGGCTGCCGG GGACTCGGCT CAGTGGCTGT CGGTGAAAGA AGAGACTATC 
    TTTTTACATG ATGGGCTGAT CCGGGTCACA GACCTGGCGG AGTTGCCCAG CGAAATCGGG
    GTCAGTGAGG CCGGGGACGC AGAACTGGAG ATATTGACTT TTGAGACTAA GAACCCTATT
    GAGCTAGCAG AGCATTTACG TGCAGTGTGT GGGAACCAGA GCAATGCCTA TGCACGATTG
    CTGGAATACC GCTTGAATGC GTTACGTGGG TTATGGAATG CCCAACGCCA GCTGGCGCTT
    GAGGATCAAC ATGAAAGAGA TGGGGCTGAT GACGAAACCT TGGCCCTGCT TAAACGGCAG
    GGTTTGTTGC AACAGCCGGA ACAGGCTCCA TTTACATCTC GAATGGGGCT TTTACTGGTG
    TTCCCCCTTA TCCAGTCCCA AAGTAAAACA GACCCTTCAC TTTGTAACTT GACAGCTGAG
    GTGCTTTTAA ATTGCCTTCG GGACTGCCAA CCCCTGAGCT TGACTAAAGA ACCAGCTGAC
    TGCCTGAATG GAATAGAGTC CTTACTGTGT TCTTGGTTAG AAGACACTCC AAATGTGGGC
    CAACAAGTCT CCTTTAAGCA AAAGGAAAAT TCTGCTGCTG CTTTGGTTGC CTTAGCTTGT
    GCTAGGGGAT CATTAAAAAC ATTTATTCAC ACTGTACACT TGCTGCAAAA ACAGACTGAC
    CTAGGATCCC TTCCTGTGGC AGATGTATTG TATAGGTTGC TGCTTTTAGA GGGAGGTCCA
    GGTTCTCCCT CTTGTTTACT GGGAAGCAAA CACATTGTAT CCTGGGGTTT TGAGGATATG
    CTTCCAGCTC CTGATGGGAT CAGTGGTTCA AACAATGAGA ACAAAGATGC AGATTTGGGT
    CGCTGTGTAG CATGCGATGG GCTTTATCTT TACACAACTA ACTCTGTAGG TAGAGGATTG
    AGCAAAGTAG GGTCTGGGCT GCATGGCACT TTACGAGGTT TTGTTTATTC CCGAAACGAG
    GAGCTAGAGG CTGGCTGGAT TGCTTATGGA AGTGGGAAGA TACTTCACAG GCCTGCCTCC
    TTTGATAACA AACCTCAATA TCTTTTCCAA TTAATTGACC AATACACACT TCAGGTCTCT
    TATATAGTCA GCATGCCAGC CAATCATTTC CCGGTTGGCA GCAATATGAC TACGGTGCAC
    CTCTCCTCGG ATGGCACGTA TTTTTACTGG ATTTGGTCTC CAGCTAGTTT AAATGAGAAA
    ACCCCCAAAG GACACTCTGT CTTCATGGAT ATATTTGAGT TAAATGTGCA AAATGGAGTA
    TGCATTGCAA GCCCTCTTCA TGAGCGCATT ATATTAATGA GGAAAGAGGG AGAATCTGCT
    AAAAGTATTA ATGAAATGCT TTTGAGCAGA TTATCACGAT ACAGAGCTTC TCCTTCAGCA
    ACTCTAGCAG CACTTACAGG ATCTACAATC TCTAACACTC TAAAAGAGGA CCAGACTGCT
    GCAAATACGA GCTGCGGGCT TCCTTTGAAA ATGTTAAGAA AGACTCCAAT GTATACTTGT
    GGCACATACT TAGTACTACT CGTGCCACCT CCGGGTGGGT CAGGAAGTTC AGCAACAAGA
    TCTTTGTTTG GTGGAACCAG CGGCCTCTCC TCTTTAAAAA TTCTGGCTTC CAGCTTGGTA
    TTTAACATTT CTGATGGGCA GTTCACCAAC AGAGCAGATC TTATTGATGC ATCTGGAAGT
    TCTCTTGGAA GGGGTGCTTT GGTGACAGGC CTAGGTGCCT GCTATGATAC AATGAATAAC
    ATGATCTGGA CGTGCTCCAA TGATTACATC GATCAGTGGT GTAATCCTGG AAACCAGGCT
    TTCCATGTGG TGTGTCAGAG GCTTGGTGTC ACTCACATCA TTGCTGATCC CAAAGGAGAG
    GCAGTATCAA CAAATGAAGT TATAAACCAG TTGCTTCACC ATGTGGGTGC TATGTGTATT
    CACCAACTCA ATCTCCTGGC TAATAGCAAC AGTTCTCCAA TCAGTACCTT TCTGGGCAAA
    CAACATCCTA TAGAGCCACA TCATCTTAAT AGTATATGTG ACATCATGGA GAAAGCCATG
    GTCAATGGTG ATAAATGCAT TATTCGCTGC ATCTTGGTTG TTTTCCAGGT GGTATTTAAA
    TTCTTTCTAA ACCCACAAAC TGAGAGGAAT CGAGAAACCA TAAGGAGGGC TGGTTTGCTC
    CTTTGGCAGT TGCTCATGGC GCCTAAGGAC CAAATTTGTC CTGAAATACA GAGAGAGGTC
    TGCTTGGCTA TTAGCTTTGG TTTAAACATT TTGAATCCAG GAGAGCTAGA AATTAATAAC
    TTGCTCAAGC TGATTTTAAC TGAAGGGGAC AGAAATTGTG GCCTCTCCCA ACTCCGTGAT
    GTCATTTTGA CAAACCTCAC TGAACAGCTT CAGAATAACA GGTTTGGAAG TGAAGATGAT
    GAACATTACA GGCTTAATGA TGAACTATTG CACTATATTT TGAAGATTGT TGTTCGTGAA
    TCCTGTGTTC TGATCACCAA GTGCCAAACA GTGTCTAAAG AAGAATTCCA AAGACTCTTG
    TCAACAGTGC CTGTGGCTTC TCCCTGTCTA TGCTATTTAA TGGCTGTACA GAATCATCTC
    CTGAGTAACA CTGTCTTAAT TAAACCTGAT GACAGCGATG ACAGTGACAA CTCCCTGCAG
    GGGGAGACAT TGAAGGTACA GGAACTCCAG GCCAGCATCT TGGCTTTGGC AACCCAGATA
    CTTTCTGGTT GTGATGAAGT TCTGGATATT CTGCAGCGAG TGACCACTGC TCTTATCAAC
    AGCGACCTGT TAGACAGAGA TTTAAGACTG AAAGGCCTTG AACAGATCAC AAAGTCCACC
    CTGCTTGGCC ATCTCCTCCC AGTACTTCTC ACATCCCTTA TGCATCCAAA CTTACAGACA
    TTAACCATGG CAGATGCGCT TATGCCTCAG CTGGTGCAGC TTGTTCTTTA CACTAGCCAG
    ACTGCACTGC TGCTTAAAAC CCAGTGTCCA GTATTGGCAG ATACAGGGGA CACCACCAGT
    GGAATTCCAG AGCAAAAAAA CAAACTGTTA ACTGATGACA GGATGCTGGA AGAAAAGGAG
    GAGCCTGGTT TCCTCACTGG GCTAAAGATT CCTGCGCCAT GGGCTGCAGG AAAGACTGTG
    GAAACATCTC ATCCAGTCAG AGACAATTAC AAGTTTAAAG AGACTGTGCA CATTCCTGGA
    GCTCGTTGTT TGTACCTCCG GTTTGACAGC AGGTGCTCTT CACAGTACGA TTATGATAAG
    CTGGTGATAT ATGCGGGACC CAACACAAAC AGCAGAAAAG TTGCCGAATA TGGTGGAAAT
    ACCCTGGGGT ATGGCAGTAG AAGTGTTTTA GGTACAGGCT GGCCAAAAGA TTTGGTTAAG
    GTGGAAGGGG ATACTGTGAC ATTCTCCTTC GAAATGAGAA GTGGGCGTGA GCACAACACT
    CCTGATAAAG CAATGTGGGG TTTTGCGTGC ACTGTCAGAG CTCAGGAGTC CTCAGAAGAT
    GTGTCAGGGG GCTTGCCATT TCTAGTAGAC CTGGCTTTAG GTCTGTCTGT CTTGGCTTGT
    TCCATGCTGC GTATATTGTA CAATGGTCCA GAAATCACAA AAGAAGAGTT GACTTGTCAC
    GATCTGTTGA GGTCAAAGCT TTTGCAGAGG TGTCAGTGGC AGGTGGAGGC TAATGGTGCT
    TTATCCCCGG CACTCACCCC AAGCCCTTCG CCGCTGCCCC TTACTATAGA AGAAGACCAA
    GAATTTACGT ATCCATCTGA CATCTTGTTG CCACCAGTTG GGAACTATTT TGAACTGCCA
    AGAATCAGAC TGCCTCCTGG TATTATGATC AAGCTCAGGG AAATATCTGG ACGCGCTCGA
    CCACAGTTTA GACCAAGCAT AAAAGAGGTT ATCCAGCCTG ATGTGATGGA AGAAATGGTT
    GTATCTTGCG TCATTAAACA CTTGAACCTG GTCGATGCAT TGCAGTCTTT AATAAACTTC
    CAGTATCGAG AAGAACATCT AGAAGAATAT GATTTATTAT GTAAAATAAT GGGGGAAACT
    TTTAAGAAGC TCAATGCAAT GGAAAGACAA TTGCAGAGTG TTGCAGAACT GGAACAAAAG
    TGGCAAAGTG AAGTGGAAGA TGCTCAGAAT GGAAAGCTGG AGAACAACGC TCCCTTTTTT
    TACGACTATC ATTTTAATGA GAGCAAGTTA AAGGAACTTG AACTTTTATG TTCTATGAAA
    GAGGTCCCTT CTGATGGAAG TGATCCAGAA AACATGGTTT TCTCTCTGCG AGAGAAGTTT
    TTTCAAGAGG TGAACTCCCT GATCCAAAAA CCTTCCCACC CAATGGCTAA GACCAAAACA
    TTGGTTAAGA GTCTGATGAA TAGAGCAGAG CTCCTGTTAC ATGTGACTAT TGCAGCACAT
    TCTGGTATCA CGAGAAGCAT ATCTGGCACT CCAACTGAAC CTCAAGCTGG CAAGTCTATC
    TCAGAAGCAA AAGTTATATC TCAAGCCATG CGCCAGCCTG TTTTCCTCCG CAGCATGTCT
    GCCCCTTCTG ACCTGGAAAT GATTGGAAGT AGTGACTTAG AGTTCAGCAG GTCTAATCCA
    AGGAGAAGGA ACATCACTAG CCACAGGAGC AGCTCCTTCA CTCTGCTTCA GTCTTTAGCT
    ATTGAAGACT GCAAGGATAA ACCAACATAC AGTGTCCTTT TAGGGCAACT GTTTGCCTTC
    ATTGGGACCA ATCCCGACCA AGCAGTATCC AGTAGCAGCT TTCTACTTGC AGCCCAAACC
    AGATGGCGTC GTGGTAATAC TCGCAAACAA GCCCTTGTAC ACATGAGAGA ATTACTGACT
    GCTGCTGTCC GTGTTGGGGG TGTGACACAT TTGGTTGGAC CAGTAACAAT GGTGCTACAG
    GGAGGACCAA GAATTGAAGA GCTCACCTGT GGAGGAATGG TGGAGCTTGT TCAAGAGGCA
    TTTGGAGAGA CCATGACCTC AGTGGTGTCA CTTTGTGCAC GGTATCCCAT TGCCTGTGCA
    AATAGCATTG GGCTTTTGTG TACTATACCT TACACAAGGA GTGAAGAAAA ATGTTTAGTT
    CGCAGTGGGC TTGTTCAACT GATGGACCGA CTCTGCAGCT TAAGCAACCA GAGAGAGTCC
    AGCTCAAATG AAAAGCAAAC CAAGAAACAG AAAGTAGCAA CTATGGCATG GGCAGCCTTC
    CAAGTACTGG CCAATAGATG TGTAGAATGG GAGAAAGAAG AAGGTGGTTC TACTGAGGCG
    GTACACTCTG GTTTGGCTAG ACAAGTCTCC ACCCTCCTCA CTAACCATCT AGCAAGGGCT
    ACAGAGTGCT GTGGAAACCA AGCTGCAGGG AATGATGCCC TACAGGACGT TCTGAGCCTT
    CTCAATGATC TCTCTAGGAG TCAGATAGGG AAGGCAATTC TGAGCCAGCC TGCTTGTGTG
    TCCAAACTTC TTTCGTTGCT GCTGGACCAG CGTCCTTCTC CAAAGCTTGT GCTCATAATT
    CTGCAGCTGT GCCGCGCTGC ACTTCCTCTC ATGAGTGTTG AAGACTGTGG AAATGTAGAA
    CTTCCTCCAT GGAGTTACTC TTTACCAGCT CTGGACAGTG AACAGGGGGA GGATCCAAGT
    GACCCAGCCT CCAAAATAGC ATCTTTGTTG CTAGCAAAGT TGGCTGATTA TGTTGTTCCA
    GGATGTCAGA CAGTTCTTTC TCCTACTGCT TCTGAACCAG ACAGCTCATT GTCCAAAAAC
    AGCCCAAAAA ATTCCCAGAA AGCAGATAAG GATGCAGAAG AAGAGAGTGA AGCAGTAGAT
    GGCAAGCTCT CCATTTTTAT TCATAAGCGA GAAGACCAGT CATCCCATGA GGTTCTTCAG
    CCTTTGCTTA GTAGTTCAGA GGGAAGGCCT TTTCGTCTTG GTACTGGAGC GAATATGGAA
    AAAGTAGTAA AGATGGATCG TGACATGACT AAGAGTGGAT GTTGTGAAGT AATCACAGAG
    GAGGCTGCAG CTGCTCTCCG CAAAGCCACT AAATGGGCCC AGTCGGGTTT AATTGTTAGC
    ATTGGGCCAC CTGTGGAGAC AGTTAGCCCT GATTCAGCGA GTGGACTTTC CACTGGTGAC
    AAAAAGAAAA CAGCCCAGAC CTCGATCTGT AGGGAAAGGA ATTCTGAACT TGCCAGGACT
    GATCCTGTAC GCCCTTTCAT TAGTGGCCAT GTAGCAAACA GCATGGCTGC AGAAGTAATT
    GCTCTGCTTC ACAGCCTGCT GATGGCACCA GAGTCAAATG CTGCCCAAAT CTGGACAACA
    ACAGCAGAGA AGGTTCTTTC TCGTGCCCTG ATGTATATCC CACAGCTTGG CAAATATGCA
    GAAAGCATCC TAGAGAACGG CAGCGGTAAT GGCAGGAAGC TAGCAAAGCT ACAAGGCATT
    GCAAGGCAAG CAGTTGCTGC ATTATGTGCG CTTGGTGGAT TTAAAGAAAC CATTAAAATA
    GGTTCTGAAG TCCAGGTGTT GGGTAAAGGA ATATCTGGGA GCATTGGAGT GGTTGTTTCT
    ATTAATGAAC AAGAAGGTAT TGCAACAGTG AAATTTCCAG ACATAGATTT TAGAAAATCA
    TGCAAAGCAT CTGACACACT GACAGTACCA TTATTTAGGC TCTGCGTTCC ACGGTCAGAG
    GCATTGCCTC TCCATAAACT GTCCATTACT GAGAAGGTAG TGCAGGCAGT CCAGTCCATG
    TTACTTCCCC AGGAGGGCTG CCTATCTATT CATACCTCGC TTCCTGCAAC AGGAGATGCC
    TCAACTCCAG TAATGGCAGT GGTTCGACTT CTTGCAGAGA TCAGGACAAG AGCTTGCTTG
    GTGATGGCTC AGCTGTTGGA GGACAGCTCA TTTTGTGAAG AATTCATACA GCAATGTCCA
    GCTGCTGTAG AAGTTCTAAA CCTAGTGGCC CAGGAATGCA GTCCTGGGGA GCGTCTCTCA
    GTGGTGGAAG TTCAGTGTGA AAGACTACGA ATGCTTTATC GGGACTGTGC TCGCCCCCCG
    CCACCACCTT TGCAGGCTGA GCGCAGACAG CCCAAGGAAA TCACCTGGTC TCCTTCCAGG
    GTATTCCCTC CTGTGAGAGC CTGTATGTTT TCATCCCATC TAACAGCAGT AACATTTCTG
    GCTGACCCAA GTGCTGGTGG GGGACTTCCT CGCGGCACAT TTATATATGC AACATCCCCT
    GTTCCAGTAC AGGCACCATC TTTTTACTGG GAAATAGAAG TAGTTTCCTA TGGAGACACA
    GATGATGACA CTGGCCCTAT TGTATCATTT GGGTTTGCTA CAGAAGCAGA GAAGCGGGAT
    GGAGCATGGA CAAACCCAGT CGGTACCTGT CTTTTTCATA ACAATGGTCG AGCTGTACAT
    TACAATGGAT CAAGTCTCTT ACAGTGGAAG AGTGTTCGAT TAGATGTGAC TCTTGCTCCT
    GGTGATGTTG CTGGAATAGG TTGGGAGAGA ACTGAAGGAA CACCACCACC CCCAGGGCAG
    CCTGCCAAGG GCAGAGTCTA CTTTACTTAC TGTGGACAGA GGCTTACTCC GTTCTTAGAG
    GATGTCTCTG GGGGCATGTG GCCAGTTGTG CATATTCAGA AAAAGAACAC TAAAGTCCGT
    GCAAATTTTG GATGCAGACA GTTTGCTTTT GCCGAGGGTC AAGCTCATCG CAATGCAGCA
    GATTTGTGCA TTGATTTGGC TGAAGAGATA AGTGCAAACT TTGAAGCATT GCCCTTTGCT
    ATGGCATCTG ACAGTGATAA TGATGCTGGG ACAAGCATAG CATCTGATCC TGGAACTCAT
    GGACCTCCTT GCCGTATTGC TGCAGTTTCT ACTGTGCAAC AGCAATATGA CAGTGATGCC
    TCATGCCGTT ACAAGGTAGA GCTGAGTTAT CAAGACTTTG TGACCTCTGG ACCTGATCCA
    CACCCACCTC CCATTGCAGA TGATGAAAGT GATGATGATG ACGATGATGA TATTCCTCGA
    GAGGATCATT ATGCTCTTTT GGTAAAAGCA TGGGAGACCA AAGTTTTTCC AACCATTCGA
    AGACGTTTCC GTAACGAGGC AGAGAGAAAG TCTGGCCTGG ACCAGATTAA GGGAGCTCTT
    CAGCTAGGAA TGGTGGATAT AGCGAGGCAA ACTGTAGAGT TCCTCTATGA AGAAAATGGT
    GGCATTCCAA GAGATCTCTA TCTTCCTACT ATTGAGGATA TCAAGGATGA GGCAAATAAA
    TTCACCATTG ATAAAGTTCG CAAAGGACTC ACAGTGGTCA TTCGATCCCC AGATAGCAAC
    AATGTAGCTG GGACAACTGT GGGAACAGCT TTACCCAAGT TTGCCATCCG TGGTATGCTG
    AAGACATTCT CTCTACATGG GGTGGTTCTG GATGTGGATT CAGTTAATGA GCTGGTTCAG
    GTAGAGACTT ACCTCCGGAG TGAAGGGGTA TTAGTGAGAT ACTGGTATCC CATAGATATG
    CTAGAAAGAC CTCCTGCGGG ATACAGGAGA ATGGCAACCA ATGGACTGGT GACTTTAGAT
    AGCACTAACA TCCAGATTCA TAGAGAGCTG TTGCGGTGTG AGTCTGCACT TGCCAGGCTT
    TACTGTCGTG TGGCTTTGCT TAACATCTTT GCTCCTAAGT CTCCGCACAC TTTCACTCGC
    CTGTTCCATA TTCCTGCTAT CCGAGACATT ACATTGGAGC ATTTACAGCT TCTGTCAAAT
    CAGCTTCTAA TGCCACCCCT TCCAGACGGA ACCATTACCT CCAGCTCTAT CCTTCTTGCC
    CAGTCCTTGC AACATTGCAT CTACTCCCAG AACTGCTCAA CAACAGATTT GTTCTACCAG
    GGCAATGCAC AGCCAATAAG AGAGTGGCTT GGTGTGGCCA TAACCAGGGC CCTGCATCAA
    GGAGAGGACA GTCTCTTGGA TCTGACAAAA CAAATCTGTT CCTTCTTGCA AAATGCGCCG
    GAACAGTTTC CATCTGAAGA ATTTCCCATC TCTGAATCCA AGCTCAGTAT GGATGTTAAT
    TTTCCTGGGG CAGCTTTTGT TATTGTTTCT TGTATAGAGA GTCCGTCTGG ATTTAGAAAA
    GACTCCTCCT TGTACAAGGC TCCCTGGTCT AGAGTCATGG TATATGGCCT TGGACATAAA
    GTAAAGAGGA ATGGACAGCT GAACCTGATA GAATCAGTTT GTTATCCACG TGATGCCTCC
    CCAAGTAACA CAGGCCTCAC TCCCCCACCA ACCACAAACC AGTACCCAAC AGCGATTATT
    CCCACTGACA AGGTCCACGT CAAACTTGGT GTATCACCTA CTCCTGGTGC TGTGCTGGTC
    ATCCATTCCC TGCCTCTAGA GTTTCCGTTG GCAATGGCAT TCACAGAACA GCTGTTAACC
    TGGAAGTTGG AGGACACAGA AGAGAAGTCA GAAGATGAGC TTGATACTGT TCCATCCTCT
    GCTCTGTTGC AAGTGGTAGA ACTTTTAGGT ACTTTGATTT GGACAACAGA CCTTGCTGCA
    GTAATTAAGG AGCTTCTGTT CCATCTGTTG GCTGAACTAT TAAGGAAAGT TCACCATCTA
    GAGCAGAAGA AGAACCCAAG TGGATTATCT TCCTCGATAG CCCTGCAGTT GAATCCATGT
    CTAGCAATGC TTATGGCTTT ACAGACAGAA CTGCGCAAGC TGTATGATGA AGAAACTCAA
    AACTGGGCAT CTGGGAATGC ATGTGGGGGG TCTGTACCTG GAGCCTCTGA GCAGGGAAGA
    TTTTCAACTT ACTTTCATGC CCTCATGGAA GTGTGCCTGG CAGTGGCAGA AGTGACGCTC
    CCAATCAACA TGAGCTCAGC AATCAGTGTG ATGGCATCTA CAAATGCCAC TAATCTGAGT
    GACTCTTCTT CTTCCTCTTC CTCTTCCCCT GGACAGACTC CTCAAAGTCC AAGTCTGTTA
    TCAAAGAGAA AGAAAGTTAA GATCAAACGT GAGAAGACTG CATCTGGAAA ACGTACACCT
    TCACGTGGTG CAGAGAGTGA TGCTGCTTTG CTTAGCATTG GAGGAAGCAA ACCTGAGGAC
    ATGTTGTGGT TCCATAGGGC ATTGACTTTA TTGATGATCC TAAGGCATCT AACTAACAAG
    GATCCACAGG GGATGGGTGT CACAAACGAT GCTATTGCTG ATGCTAGCCA GGCTTTAGTT
    GGTCCCACAG CTCACAGTCG TCTCCTTGTG ATTTCTGGCA TCCCTACACA CCTAGAAGAA
    TCAGTGGTCA GAAGTGCAAT TAGAAAGGCA TGTAATGCTC ATGGTGGAGT CTTTAAAGAT
    GAAATCTACA TTCCATTGCA GGAAACTGAA ACCCCTAAAC CCAAAGACAG AATAGAAGGG
    GCAGAATGCA AGACAGAAAT AGAAAAACCC TCTACGCAGC ATACAACCGA CAATATGGAT
    ATCAGCAGCT CGAGCAGTGT AACTCCTGCA GCAAGTGTCA CGGCATCAGC TTCTACAAGC
    CAAGCTTCAT TGTGCAGCTC CCAAGGGGGG GTATCACGGA CCACAAGTGA AACATCTATG
    GAGCAGTTTG CCCCAGAACA TGCTGTGCCA TCAGGGCTTC TGGAACCAAC TATTATCTCC
    AGCCAGGAGA GTCTGGATCT TTCTATGTGC AGCACAGGAA GCCTCGGCAG TCTGGGAAGT
    CTTGTTGAAC AGCTTGATAA TGTTGAAAAC TCTTTGTCTG ACGTGGGCTC CCTATACACA
    GCTACATCAC TAGATAGCCA TATGGCAGAG TCGCGGCCAA TCAAAGGATT TGCAGTTGTA
    GAGGTGCGAT CACGAGCTAA AATAGAGAAA ATCCGTGCCA CTTTATTTAA CAGTAGTGAC
    CTCATTGGGT TATCTGGTTT GGATGGTGAA GATGAGCTGA TGGAAATGTC TACTGAGGAG
    ATCTTGACTG TTTCAACAGT GAATCAGAGC TTGTTTGATA CCCAAGGAAT ACCCGGTCTG
    GCTGATTATT TCATGGATAA ATCAATGAAA GGTGATAAAT TAGTCCCGGA AGCTCGTGAT
    GTTCTGACTG AGATTTTCAA GAGCTGCATT CACGCCGAGC AGATGCTGAG TCTGACTCCA
    GCCAAGTTTA TCAAAGTGTC TGATATCTAC CTTAGCAAGG AGCAAATCAA TTCACAGACC
    CCAGGGAACT TACTGCATAC CTTTTTCACC AATGTTCGAC CACCTAAAAA AGTCCTGGAA
    GATCAACTAA CGCAGATCTT AAGAAAATAT GGAATTCCCA AGCCTAAATT TGACAAAAGC
    AAATACAGCA AAACTGGGAA GGAGCAACAT CCTGTGAAAG TTGTAAGCAC CAAAAGACCT
    GTGGGCAAAC CACCGACCAA AGACAAGGCT GTATTCAACA GCGTCAGGAC TGCTTTAAGT
    GAGAAAAAAC CAACTGTGAA GCCAAAATCT CCGGAGAAGG GCAGGCCAGA TGAGAAGGAC
    CCAGACAAAT CTCCAACAAA GAAGCAAGAA ATTCCCGAGG AGAGATATCT GACGTTGGAT
    GGATTTCACC GCTTTGTAGT AGATCGGGCT AAGCAAGACA TTCGAAGCAT CTGGAGAGCT
    GTCTTGGCGT GTGGATATGA TCTTCATTTT GAAAGGTGTG CCTGCATTGA TACAAGGCAT
    GCTCAGAAAT CTAGCAGAAA ATGGACCTTG GAAATGGATG TTGCTCTTGT TCAGTATATC
    AACCGCCTTT GCCGTCACCT TGCCATAACG CCAGCCCGAC TACACCCTCA TGAGGTTTAT
    CTGGATCTTG CAGATACGGC TGACCCAAGA GTCTCCTGCC TGCTGAATGT ACCAATAGAA
    AGCCTGCGCC TCCGATTTGC TCTTCTTCAG TCCCTCAACA CTACTTTAGA GACCTTTTTC
    CTGCCCTTAG TGGAGCTACG GCAAACAGAC GTATACACAC ACAGCATCGC ATCCCTACTC
    CAGCTAGCTA AAGGGTTAAT CTTTTACGAT ACAAAAGTGA ATGTGATGAA CCGGGTATTG
    AACGCAACAG TGCAGAGGAC AGCAGATCAT GCAGCCCCTG AGATCACGCT GGACCCACTA
    GAAATTGTAG GAGGGGAAAT TCGTACCTCA GAAAACTCCT ACTTCTGCCA GGCAGCCCGA
    CAGTTGGCAT GCATCCCTTC CTCACAACTG TGTGTCAAAC TGGCAAGTGG AGGAGACCCC
    ACGTATGCAT TTAACATCCG CTTTACTGGA GAGGAGGTGC ATGGTACAAG TGGCTCATTC
    CGTCATTTCC TCTGGCAAGT GTGTAAAGAA CTACAGAGCT CCTCGCTGTC TCTTCTACTG
    CTCTGCCCCA GCTCTGCAGT TAATAAGAAT AAGGGAAAAT ATCTTCTAAC TCCCAGTCCC
    ATAACATATG CAGAAGAGCA GTTGCTCCAT TTCTTTGGCC AACTACTGGG AATAGCCATC
    AGAGCCGATG TTCCACTCCC TCTGGATCTA CTCCCCTGCT TCTGGAAGAT GCTTGTAGGA
    GAACCTTTGG ACCCAGAAAC TGATCTTTAT GAAGCAGATA TTCTCACCCA CAACTACATC
    AAGAAGTTTG AAAATGTAAA TGATGAGTCA GAGCTGGAAG CTCTTTGTGC TGAAATTGCC
    TCTCAGCATC TTCCTACCGA AAGTCCAGAT TCTCCTCATA AACCAGCCTG CAAATTTACA
    TACTTGACAA TGACTGGTGA AGAAGTGGAG CTGTGCCCTA AGGGAAGACA CATTTCTGTA
    GTCTGGGAAA ACAAAGATAT ATATGCAATG GGAATCCGGA ACCTTCGTAT ACGTGAGCTG
    AAGAACCAGG AGTGTATGAG CGCAGTAAGG GCTGGGCTGG GCTCCATTAT ACCTCTGCAG
    CTCCTCACCA CTCTAAATGC TCTAGAGATG GAACTAAGGA CATGTGGCCT ACCATATATT
    AATTTGGAAT TCTTAAAGGC TCACACCATG TATCAAGTGG GTCTAATGGA GACAGATCAA
    CACATTGAGT ATTTTTGGTC TGCCTTAGAA ATGTTCACTC AGGAAGAACT TTGCAAATTC
    ATAAAATTCG CCTGCAATCA GGAACGCATT CCCTTTACAT GTCCATGCAA AGATGGTGGG
    CCAGACACGG CTCATGTTCC ACCTTATCCT ATGAAAATAG CACCACCCGA TGGAGCAACA
    GGCTCCCCAG ATTCCAGGTA CATCCGAGTG GAGACATGTA TGTTCATGAT CAAGCTTCCG
    CAGTACTCGT CTCTAGAGAT GATGCTGGAG AAACTGAGAT ATGCCATTCA CTACAGGGAA
    GACCCACTCA GCGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012824253.1
    CDS323..13540
    Translation

    Target ORF information:

    RefSeq Version XM_012968799.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 4 (hectd4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012968799.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGGGGTCGG CGGCTGCCGG GGACTCGGCT CAGTGGCTGT CGGTGAAAGA AGAGACTATC 
    TTTTTACATG ATGGGCTGAT CCGGGTCACA GACCTGGCGG AGTTGCCCAG CGAAATCGGG
    GTCAGTGAGG CCGGGGACGC AGAACTGGAG ATATTGACTT TTGAGACTAA GAACCCTATT
    GAGCTAGCAG AGCATTTACG TGCAGTGTGT GGGAACCAGA GCAATGCCTA TGCACGATTG
    CTGGAATACC GCTTGAATGC GTTACGTGGG TTATGGAATG CCCAACGCCA GCTGGCGCTT
    GAGGATCAAC ATGAAAGAGA TGGGGCTGAT GACGAAACCT TGGCCCTGCT TAAACGGCAG
    GGTTTGTTGC AACAGCCGGA ACAGGCTCCA TTTACATCTC GAATGGGGCT TTTACTGGTG
    TTCCCCCTTA TCCAGTCCCA AAGTAAAACA GACCCTTCAC TTTGTAACTT GACAGCTGAG
    GTGCTTTTAA ATTGCCTTCG GGACTGCCAA CCCCTGAGCT TGACTAAAGA ACCAGCTGAC
    TGCCTGAATG GAATAGAGTC CTTACTGTGT TCTTGGTTAG AAGACACTCC AAATGTGGGC
    CAACAAGTCT CCTTTAAGCA AAAGGAAAAT TCTGCTGCTG CTTTGGTTGC CTTAGCTTGT
    GCTAGGGGAT CATTAAAAAC ATTTATTCAC ACTGTACACT TGCTGCAAAA ACAGACTGAC
    CTAGGATCCC TTCCTGTGGC AGATGTATTG TATAGGTTGC TGCTTTTAGA GGGAGGTCCA
    GGTTCTCCCT CTTGTTTACT GGGAAGCAAA CACATTGTAT CCTGGGGTTT TGAGGATATG
    CTTCCAGCTC CTGATGGGAT CAGTGGTTCA AACAATGAGA ACAAAGATGC AGATTTGGGT
    CGCTGTGTAG CATGCGATGG GCTTTATCTT TACACAACTA ACTCTGTAGG TAGAGGATTG
    AGCAAAGTAG GGTCTGGGCT GCATGGCACT TTACGAGGTT TTGTTTATTC CCGAAACGAG
    GAGCTAGAGG CTGGCTGGAT TGCTTATGGA AGTGGGAAGA TACTTCACAG GCCTGCCTCC
    TTTGATAACA AACCTCAATA TCTTTTCCAA TTAATTGACC AATACACACT TCAGGTCTCT
    TATATAGTCA GCATGCCAGC CAATCATTTC CCGGTTGGCA GCAATATGAC TACGGTGCAC
    CTCTCCTCGG ATGGCACGTA TTTTTACTGG ATTTGGTCTC CAGCTAGTTT AAATGAGAAA
    ACCCCCAAAG GACACTCTGT CTTCATGGAT ATATTTGAGT TAAATGTGCA AAATGGAGTA
    TGCATTGCAA GCCCTCTTCA TGAGCGCATT ATATTAATGA GGAAAGAGGG AGAATCTGCT
    AAAAGTATTA ATGAAATGCT TTTGAGCAGA TTATCACGAT ACAGAGCTTC TCCTTCAGCA
    ACTCTAGCAG CACTTACAGG ATCTACAATC TCTAACACTC TAAAAGAGGA CCAGACTGCT
    GCAAATACGA GCTGCGGGCT TCCTTTGAAA ATGTTAAGAA AGACTCCAAT GTATACTTGT
    GGCACATACT TAGTACTACT CGTGCCACCT CCGGGTGGGT CAGGAAGTTC AGCAACAAGA
    TCTTTGTTTG GTGGAACCAG CGGCCTCTCC TCTTTAAAAA TTCTGGCTTC CAGCTTGGTA
    TTTAACATTT CTGATGGGCA GTTCACCAAC AGAGCAGATC TTATTGATGC ATCTGGAAGT
    TCTCTTGGAA GGGGTGCTTT GGTGACAGGC CTAGGTGCCT GCTATGATAC AATGAATAAC
    ATGATCTGGA CGTGCTCCAA TGATTACATC GATCAGTGGT GTAATCCTGG AAACCAGGCT
    TTCCATGTGG TGTGTCAGAG GCTTGGTGTC ACTCACATCA TTGCTGATCC CAAAGGAGAG
    GCAGTATCAA CAAATGAAGT TATAAACCAG TTGCTTCACC ATGTGGGTGC TATGTGTATT
    CACCAACTCA ATCTCCTGGC TAATAGCAAC AGTTCTCCAA TCAGTACCTT TCTGGGCAAA
    CAACATCCTA TAGAGCCACA TCATCTTAAT AGTATATGTG ACATCATGGA GAAAGCCATG
    GTCAATGGTG ATAAATGCAT TATTCGCTGC ATCTTGGTTG TTTTCCAGGT GGTATTTAAA
    TTCTTTCTAA ACCCACAAAC TGAGAGGAAT CGAGAAACCA TAAGGAGGGC TGGTTTGCTC
    CTTTGGCAGT TGCTCATGGC GCCTAAGGAC CAAATTTGTC CTGAAATACA GAGAGAGGTC
    TGCTTGGCTA TTAGCTTTGG TTTAAACATT TTGAATCCAG GAGAGCTAGA AATTAATAAC
    TTGCTCAAGC TGATTTTAAC TGAAGGGGAC AGAAATTGTG GCCTCTCCCA ACTCCGTGAT
    GTCATTTTGA CAAACCTCAC TGAACAGCTT CAGAATAACA GGTTTGGAAG TGAAGATGAT
    GAACATTACA GGCTTAATGA TGAACTATTG CACTATATTT TGAAGATTGT TGTTCGTGAA
    TCCTGTGTTC TGATCACCAA GTGCCAAACA GTGTCTAAAG AAGAATTCCA AAGACTCTTG
    TCAACAGTGC CTGTGGCTTC TCCCTGTCTA TGCTATTTAA TGGCTGTACA GAATCATCTC
    CTGAGTAACA CTGTCTTAAT TAAACCTGAT GACAGCGATG ACAGTGACAA CTCCCTGCAG
    GGGGAGACAT TGAAGGTACA GGAACTCCAG GCCAGCATCT TGGCTTTGGC AACCCAGATA
    CTTTCTGGTT GTGATGAAGT TCTGGATATT CTGCAGCGAG TGACCACTGC TCTTATCAAC
    AGCGACCTGT TAGACAGAGA TTTAAGACTG AAAGGCCTTG AACAGATCAC AAAGTCCACC
    CTGCTTGGCC ATCTCCTCCC AGTACTTCTC ACATCCCTTA TGCATCCAAA CTTACAGACA
    TTAACCATGG CAGATGCGCT TATGCCTCAG CTGGTGCAGC TTGTTCTTTA CACTAGCCAG
    ACTGCACTGC TGCTTAAAAC CCAGTGTCCA GTATTGGCAG ATACAGGGGA CACCACCAGT
    GGAATTCCAG AGCAAAAAAA CAAACTGTTA ACTGATGACA GGATGCTGGA AGAAAAGGAG
    GAGCCTGGTT TCCTCACTGG GCTAAAGATT CCTGCGCCAT GGGCTGCAGG AAAGACTGTG
    GAAACATCTC ATCCAGTCAG AGACAATTAC AAGTTTAAAG AGACTGTGCA CATTCCTGGA
    GCTCGTTGTT TGTACCTCCG GTTTGACAGC AGGTGCTCTT CACAGTACGA TTATGATAAG
    CTGGTGATAT ATGCGGGACC CAACACAAAC AGCAGAAAAG TTGCCGAATA TGGTGGAAAT
    ACCCTGGGGT ATGGCAGTAG AAGTGTTTTA GGTACAGGCT GGCCAAAAGA TTTGGTTAAG
    GTGGAAGGGG ATACTGTGAC ATTCTCCTTC GAAATGAGAA GTGGGCGTGA GCACAACACT
    CCTGATAAAG CAATGTGGGG TTTTGCGTGC ACTGTCAGAG CTCAGGAGTC CTCAGAAGAT
    GTGTCAGGGG GCTTGCCATT TCTAGTAGAC CTGGCTTTAG GTCTGTCTGT CTTGGCTTGT
    TCCATGCTGC GTATATTGTA CAATGGTCCA GAAATCACAA AAGAAGAGTT GACTTGTCAC
    GATCTGTTGA GGTCAAAGCT TTTGCAGAGG TGTCAGTGGC AGGTGGAGGC TAATGGTGCT
    TTATCCCCGG CACTCACCCC AAGCCCTTCG CCGCTGCCCC TTACTATAGA AGAAGACCAA
    GAATTTACGT ATCCATCTGA CATCTTGTTG CCACCAGTTG GGAACTATTT TGAACTGCCA
    AGAATCAGAC TGCCTCCTGG TATTATGATC AAGCTCAGGG AAATATCTGG ACGCGCTCGA
    CCACAGTTTA GACCAAGCAT AAAAGAGGTT ATCCAGCCTG ATGTGATGGA AGAAATGGTT
    GTATCTTGCG TCATTAAACA CTTGAACCTG GTCGATGCAT TGCAGTCTTT AATAAACTTC
    CAGTATCGAG AAGAACATCT AGAAGAATAT GATTTATTAT GTAAAATAAT GGGGGAAACT
    TTTAAGAAGC TCAATGCAAT GGAAAGACAA TTGCAGAGTG TTGCAGAACT GGAACAAAAG
    TGGCAAAGTG AAGTGGAAGA TGCTCAGAAT GGAAAGCTGG AGAACAACGC TCCCTTTTTT
    TACGACTATC ATTTTAATGA GAGCAAGTTA AAGGAACTTG AACTTTTATG TTCTATGAAA
    GAGGTCCCTT CTGATGGAAG TGATCCAGAA AACATGGTTT TCTCTCTGCG AGAGAAGTTT
    TTTCAAGAGG TGAACTCCCT GATCCAAAAA CCTTCCCACC CAATGGCTAA GACCAAAACA
    TTGGTTAAGA GTCTGATGAA TAGAGCAGAG CTCCTGTTAC ATGTGACTAT TGCAGCACAT
    TCTGGTATCA CGAGAAGCAT ATCTGGCACT CCAACTGAAC CTCAAGCTGG CAAGTCTATC
    TCAGAAGCAA AAGTTATATC TCAAGCCATG CGCCAGCCTG TTTTCCTCCG CAGCATGTCT
    GCCCCTTCTG ACCTGGAAAT GATTGGAAGT AGTGACTTAG AGTTCAGCAG GTCTAATCCA
    AGGAGAAGGA ACATCACTAG CCACAGGAGC AGCTCCTTCA CTCTGCTTCA GTCTTTAGCT
    ATTGAAGACT GCAAGGATAA ACCAACATAC AGTGTCCTTT TAGGGCAACT GTTTGCCTTC
    ATTGGGACCA ATCCCGACCA AGCAGTATCC AGTAGCAGCT TTCTACTTGC AGCCCAAACC
    AGATGGCGTC GTGGTAATAC TCGCAAACAA GCCCTTGTAC ACATGAGAGA ATTACTGACT
    GCTGCTGTCC GTGTTGGGGG TGTGACACAT TTGGTTGGAC CAGTAACAAT GGTGCTACAG
    GGAGGACCAA GAATTGAAGA GCTCACCTGT GGAGGAATGG TGGAGCTTGT TCAAGAGGCA
    TTTGGAGAGA CCATGACCTC AGTGGTGTCA CTTTGTGCAC GGTATCCCAT TGCCTGTGCA
    AATAGCATTG GGCTTTTGTG TACTATACCT TACACAAGGA GTGAAGAAAA ATGTTTAGTT
    CGCAGTGGGC TTGTTCAACT GATGGACCGA CTCTGCAGCT TAAGCAACCA GAGAGAGTCC
    AGCTCAAATG AAAAGCAAAC CAAGAAACAG AAAGTAGCAA CTATGGCATG GGCAGCCTTC
    CAAGTACTGG CCAATAGATG TGTAGAATGG GAGAAAGAAG AAGGTGGTTC TACTGAGGCG
    GTACACTCTG GTTTGGCTAG ACAAGTCTCC ACCCTCCTCA CTAACCATCT AGCAAGGGCT
    ACAGAGTGCT GTGGAAACCA AGCTGCAGGG AATGATGCCC TACAGGACGT TCTGAGCCTT
    CTCAATGATC TCTCTAGGAG TCAGATAGGG AAGGCAATTC TGAGCCAGCC TGCTTGTGTG
    TCCAAACTTC TTTCGTTGCT GCTGGACCAG CGTCCTTCTC CAAAGCTTGT GCTCATAATT
    CTGCAGCTGT GCCGCGCTGC ACTTCCTCTC ATGAGTGTTG AAGACTGTGG AAATGTAGAA
    CTTCCTCCAT GGAGTTACTC TTTACCAGCT CTGGACAGTG AACAGGGGGA GGATCCAAGT
    GACCCAGCCT CCAAAATAGC ATCTTTGTTG CTAGCAAAGT TGGCTGATTA TGTTGTTCCA
    GGATGTCAGA CAGTTCTTTC TCCTACTGCT TCTGAACCAG ACAGCTCATT GTCCAAAAAC
    AGCCCAAAAA ATTCCCAGAA AGCAGATAAG GATGCAGAAG AAGAGAGTGA AGCAGTAGAT
    GGCAAGCTCT CCATTTTTAT TCATAAGCGA GAAGACCAGT CATCCCATGA GGTTCTTCAG
    CCTTTGCTTA GTAGTTCAGA GGGAAGGCCT TTTCGTCTTG GTACTGGAGC GAATATGGAA
    AAAGTAGTAA AGATGGATCG TGACATGACT AAGAGTGGAT GTTGTGAAGT AATCACAGAG
    GAGGCTGCAG CTGCTCTCCG CAAAGCCACT AAATGGGCCC AGTCGGGTTT AATTGTTAGC
    ATTGGGCCAC CTGTGGAGAC AGTTAGCCCT GATTCAGCGA GTGGACTTTC CACTGGTGAC
    AAAAAGAAAA CAGCCCAGAC CTCGATCTGT AGGGAAAGGA ATTCTGAACT TGCCAGGACT
    GATCCTGTAC GCCCTTTCAT TAGTGGCCAT GTAGCAAACA GCATGGCTGC AGAAGTAATT
    GCTCTGCTTC ACAGCCTGCT GATGGCACCA GAGTCAAATG CTGCCCAAAT CTGGACAACA
    ACAGCAGAGA AGGTTCTTTC TCGTGCCCTG ATGTATATCC CACAGCTTGG CAAATATGCA
    GAAAGCATCC TAGAGAACGG CAGCGGTAAT GGCAGGAAGC TAGCAAAGCT ACAAGGCATT
    GCAAGGCAAG CAGTTGCTGC ATTATGTGCG CTTGGTGGAT TTAAAGAAAC CATTAAAATA
    GGTTCTGAAG TCCAGGTGTT GGGTAAAGGA ATATCTGGGA GCATTGGAGT GGTTGTTTCT
    ATTAATGAAC AAGAAGGTAT TGCAACAGTG AAATTTCCAG ACATAGATTT TAGAAAATCA
    TGCAAAGCAT CTGACACACT GACAGTACCA TTATTTAGGC TCTGCGTTCC ACGGTCAGAG
    GCATTGCCTC TCCATAAACT GTCCATTACT GAGAAGGTAG TGCAGGCAGT CCAGTCCATG
    TTACTTCCCC AGGAGGGCTG CCTATCTATT CATACCTCGC TTCCTGCAAC AGGAGATGCC
    TCAACTCCAG TAATGGCAGT GGTTCGACTT CTTGCAGAGA TCAGGACAAG AGCTTGCTTG
    GTGATGGCTC AGCTGTTGGA GGACAGCTCA TTTTGTGAAG AATTCATACA GCAATGTCCA
    GCTGCTGTAG AAGTTCTAAA CCTAGTGGCC CAGGAATGCA GTCCTGGGGA GCGTCTCTCA
    GTGGTGGAAG TTCAGTGTGA AAGACTACGA ATGCTTTATC GGGACTGTGC TCGCCCCCCG
    CCACCACCTT TGCAGGCTGA GCGCAGACAG CCCAAGGAAA TCACCTGGTC TCCTTCCAGG
    GTATTCCCTC CTGTGAGAGC CTGTATGTTT TCATCCCATC TAACAGCAGT AACATTTCTG
    GCTGACCCAA GTGCTGGTGG GGGACTTCCT CGCGGCACAT TTATATATGC AACATCCCCT
    GTTCCAGTAC AGGCACCATC TTTTTACTGG GAAATAGAAG TAGTTTCCTA TGGAGACACA
    GATGATGACA CTGGCCCTAT TGTATCATTT GGGTTTGCTA CAGAAGCAGA GAAGCGGGAT
    GGAGCATGGA CAAACCCAGT CGGTACCTGT CTTTTTCATA ACAATGGTCG AGCTGTACAT
    TACAATGGAT CAAGTCTCTT ACAGTGGAAG AGTGTTCGAT TAGATGTGAC TCTTGCTCCT
    GGTGATGTTG CTGGAATAGG TTGGGAGAGA ACTGAAGGAA CACCACCACC CCCAGGGCAG
    CCTGCCAAGG GCAGAGTCTA CTTTACTTAC TGTGGACAGA GGCTTACTCC GTTCTTAGAG
    GATGTCTCTG GGGGCATGTG GCCAGTTGTG CATATTCAGA AAAAGAACAC TAAAGTCCGT
    GCAAATTTTG GATGCAGACA GTTTGCTTTT GCCGAGGGTC AAGCTCATCG CAATGCAGCA
    GATTTGTGCA TTGATTTGGC TGAAGAGATA AGTGCAAACT TTGAAGCATT GCCCTTTGCT
    ATGGCATCTG ACAGTGATAA TGATGCTGGG ACAAGCATAG CATCTGATCC TGGAACTCAT
    GGACCTCCTT GCCGTATTGC TGCAGTTTCT ACTGTGCAAC AGCAATATGA CAGTGATGCC
    TCATGCCGTT ACAAGGTAGA GCTGAGTTAT CAAGACTTTG TGACCTCTGG ACCTGATCCA
    CACCCACCTC CCATTGCAGA TGATGAAAGT GATGATGATG ACGATGATGA TATTCCTCGA
    GAGGATCATT ATGCTCTTTT GGTAAAAGCA TGGGAGACCA AAGTTTTTCC AACCATTCGA
    AGACGTTTCC GTAACGAGGC AGAGAGAAAG TCTGGCCTGG ACCAGATTAA GGGAGCTCTT
    CAGCTAGGAA TGGTGGATAT AGCGAGGCAA ACTGTAGAGT TCCTCTATGA AGAAAATGGT
    GGCATTCCAA GAGATCTCTA TCTTCCTACT ATTGAGGATA TCAAGGATGA GGCAAATAAA
    TTCACCATTG ATAAAGTTCG CAAAGGACTC ACAGTGGTCA TTCGATCCCC AGATAGCAAC
    AATGTAGCTG GGACAACTGT GGGAACAGCT TTACCCAAGT TTGCCATCCG TGGTATGCTG
    AAGACATTCT CTCTACATGG GGTGGTTCTG GATGTGGATT CAGTTAATGA GCTGGTTCAG
    GTAGAGACTT ACCTCCGGAG TGAAGGGGTA TTAGTGAGAT ACTGGTATCC CATAGATATG
    CTAGAAAGAC CTCCTGCGGG ATACAGGAGA ATGGCAACCA ATGGACTGGT GACTTTAGAT
    AGCACTAACA TCCAGATTCA TAGAGAGCTG TTGCGGTGTG AGTCTGCACT TGCCAGGCTT
    TACTGTCGTG TGGCTTTGCT TAACATCTTT GCTCCTAAGT CTCCGCACAC TTTCACTCGC
    CTGTTCCATA TTCCTGCTAT CCGAGACATT ACATTGGAGC ATTTACAGCT TCTGTCAAAT
    CAGCTTCTAA TGCCACCCCT TCCAGACGGA ACCATTACCT CCAGCTCTAT CCTTCTTGCC
    CAGTCCTTGC AACATTGCAT CTACTCCCAG AACTGCTCAA CAACAGATTT GTTCTACCAG
    GGCAATGCAC AGCCAATAAG AGAGTGGCTT GGTGTGGCCA TAACCAGGGC CCTGCATCAA
    GGAGAGGACA GTCTCTTGGA TCTGACAAAA CAAATCTGTT CCTTCTTGCA AAATGCGCCG
    GAACAGTTTC CATCTGAAGA ATTTCCCATC TCTGAATCCA AGCTCAGTAT GGATGTTAAT
    TTTCCTGGGG CAGCTTTTGT TATTGTTTCT TGTATAGAGA GTCCGTCTGG ATTTAGAAAA
    GACTCCTCCT TGTACAAGGC TCCCTGGTCT AGAGTCATGG TATATGGCCT TGGACATAAA
    GTAAAGAGGA ATGGACAGCT GAACCTGATA GAATCAGTTT GTTATCCACG TGATGCCTCC
    CCAAGTAACA CAGGCCTCAC TCCCCCACCA ACCACAAACC AGTACCCAAC AGCGATTATT
    CCCACTGACA AGGTCCACGT CAAACTTGGT GTATCACCTA CTCCTGGTGC TGTGCTGGTC
    ATCCATTCCC TGCCTCTAGA GTTTCCGTTG GCAATGGCAT TCACAGAACA GCTGTTAACC
    TGGAAGTTGG AGGACACAGA AGAGAAGTCA GAAGATGAGC TTGATACTGT TCCATCCTCT
    GCTCTGTTGC AAGTGGTAGA ACTTTTAGGT ACTTTGATTT GGACAACAGA CCTTGCTGCA
    GTAATTAAGG AGCTTCTGTT CCATCTGTTG GCTGAACTAT TAAGGAAAGT TCACCATCTA
    GAGCAGAAGA AGAACCCAAG TGGATTATCT TCCTCGATAG CCCTGCAGTT GAATCCATGT
    CTAGCAATGC TTATGGCTTT ACAGACAGAA CTGCGCAAGC TGTATGATGA AGAAACTCAA
    AACTGGGCAT CTGGGAATGC ATGTGGGGGG TCTGTACCTG GAGCCTCTGA GCAGGGAAGA
    TTTTCAACTT ACTTTCATGC CCTCATGGAA GTGTGCCTGG CAGTGGCAGA AGTGACGCTC
    CCAATCAACA TGAGCTCAGC AATCAGTGTG ATGGCATCTA CAAATGCCAC TAATCTGAGT
    GACTCTTCTT CTTCCTCTTC CTCTTCCCCT GGACAGACTC CTCAAAGTCC AAGTCTGTTA
    TCAAAGAGAA AGAAAGTTAA GATCAAACGT GAGAAGACTG CATCTGGAAA ACGTACACCT
    TCACGTGGTG CAGAGAGTGA TGCTGCTTTG CTTAGCATTG GAGGAAGCAA ACCTGAGGAC
    ATGTTGTGGT TCCATAGGGC ATTGACTTTA TTGATGATCC TAAGGCATCT AACTAACAAG
    GATCCACAGG GGATGGGTGT CACAAACGAT GCTATTGCTG ATGCTAGCCA GGCTTTAGTT
    GGTCCCACAG CTCACAGTCG TCTCCTTGTG ATTTCTGGCA TCCCTACACA CCTAGAAGAA
    TCAGTGGTCA GAAGTGCAAT TAGAAAGGCA TGTAATGCTC ATGGTGGAGT CTTTAAAGAT
    GAAATCTACA TTCCATTGCA GGAAACTGAA ACCCCTAAAC CCAAAGACAG AATAGAAGGG
    GCAGAATGCA AGACAGAAAT AGAAAAACCC TCTACGCAGC ATACAACCGA CAATATGGAT
    ATCAGCAGCT CGAGCAGTGT AACTCCTGCA GCAAGTGTCA CGGCATCAGC TTCTACAAGC
    CAAGCTTCAT TGTGCAGCTC CCAAGGGGGG GTATCACGGA CCACAAGTGA AACATCTATG
    GAGCAGTTTG CCCCAGAACA TGCTGTGCCA TCAGGGCTTC TGGAACCAAC TATTATCTCC
    AGCCAGGAGA GTCTGGATCT TTCTATGTGC AGCACAGGAA GCCTCGGCAG TCTGGGAAGT
    CTTGTTGAAC AGCTTGATAA TGTTGAAAAC TCTTTGTCTG ACGTGGGCTC CCTATACACA
    GCTACATCAC TAGATAGCCA TATGGCAGAG TCGCGGCCAA TCAAAGGATT TGCAGTTGTA
    GAGGTGCGAT CACGAGCTAA AATAGAGAAA ATCCGTGCCA CTTTATTTAA CAGTAGTGAC
    CTCATTGGGT TATCTGGTTT GGATGGTGAA GATGAGCTGA TGGAAATGTC TACTGAGGAG
    ATCTTGACTG TTTCAACAGT GAATCAGAGC TTGTTTGATA CCCAAGGAAT ACCCGGTCTG
    GCTGATTATT TCATGGATAA ATCAATGAAA GGTGATAAAT TAGTCCCGGA AGCTCGTGAT
    GTTCTGACTG AGATTTTCAA GAGCTGCATT CACGCCGAGC AGATGCTGAG TCTGACTCCA
    GCCAAGTTTA TCAAAGTGTC TGATATCTAC CTTAGCAAGG AGCAAATCAA TTCACAGACC
    CCAGGGAACT TACTGCATAC CTTTTTCACC AATGTTCGAC CACCTAAAAA AGTCCTGGAA
    GATCAACTAA CGCAGATCTT AAGAAAATAT GGAATTCCCA AGCCTAAATT TGACAAAAGC
    AAATACAGCA AAACTGGGAA GGAGCAACAT CCTGTGAAAG TTGTAAGCAC CAAAAGACCT
    GTGGGCAAAC CACCGACCAA AGACAAGGCT GTATTCAACA GCGTCAGGAC TGCTTTAAGT
    GAGAAAAAAC CAACTGTGAA GCCAAAATCT CCGGAGAAGG GCAGGCCAGA TGAGAAGGAC
    CCAGACAAAT CTCCAACAAA GAAGCAAGAA ATTCCCGAGG AGAGATATCT GACGTTGGAT
    GGATTTCACC GCTTTGTAGT AGATCGGGCT AAGCAAGACA TTCGAAGCAT CTGGAGAGCT
    GTCTTGGCGT GTGGATATGA TCTTCATTTT GAAAGGTGTG CCTGCATTGA TACAAGGCAT
    GCTCAGAAAT CTAGCAGAAA ATGGACCTTG GAAATGGATG TTGCTCTTGT TCAGTATATC
    AACCGCCTTT GCCGTCACCT TGCCATAACG CCAGCCCGAC TACACCCTCA TGAGGTTTAT
    CTGGATCTTG CAGATACGGC TGACCCAAGA GTCTCCTGCC TGCTGAATGT ACCAATAGAA
    AGCCTGCGCC TCCGATTTGC TCTTCTTCAG TCCCTCAACA CTACTTTAGA GACCTTTTTC
    CTGCCCTTAG TGGAGCTACG GCAAACAGAC GTATACACAC ACAGCATCGC ATCCCTACTC
    CAGCTAGCTA AAGGGTTAAT CTTTTACGAT ACAAAAGTGA ATGTGATGAA CCGGGTATTG
    AACGCAACAG TGCAGAGGAC AGCAGATCAT GCAGCCCCTG AGATCACGCT GGACCCACTA
    GAAATTGTAG GAGGGGAAAT TCGTACCTCA GAAAACTCCT ACTTCTGCCA GGCAGCCCGA
    CAGTTGGCAT GCATCCCTTC CTCACAACTG TGTGTCAAAC TGGCAAGTGG AGGAGACCCC
    ACGTATGCAT TTAACATCCG CTTTACTGGA GAGGAGGTGC ATGGTACAAG TGGCTCATTC
    CGTCATTTCC TCTGGCAAGT GTGTAAAGAA CTACAGAGCT CCTCGCTGTC TCTTCTACTG
    CTCTGCCCCA GCTCTGCAGT TAATAAGAAT AAGGGAAAAT ATCTTCTAAC TCCCAGTCCC
    ATAACATATG CAGAAGAGCA GTTGCTCCAT TTCTTTGGCC AACTACTGGG AATAGCCATC
    AGAGCCGATG TTCCACTCCC TCTGGATCTA CTCCCCTGCT TCTGGAAGAT GCTTGTAGGA
    GAACCTTTGG ACCCAGAAAC TGATCTTTAT GAAGCAGATA TTCTCACCCA CAACTACATC
    AAGAAGTTTG AAAATGTAAA TGATGAGTCA GAGCTGGAAG CTCTTTGTGC TGAAATTGCC
    TCTCAGCATC TTCCTACCGA AAGTCCAGAT TCTCCTCATA AACCAGCCTG CAAATTTACA
    TACTTGACAA TGACTGGTGA AGAAGTGGAG CTGTGCCCTA AGGGAAGACA CATTTCTGTA
    GTCTGGGAAA ACAAAGATAT ATATGCAATG GGAATCCGGA ACCTTCGTAT ACGTGAGCTG
    AAGAACCAGG AGTGTATGAG CGCAGTAAGG GCTGGGCTGG GCTCCATTAT ACCTCTGCAG
    CTCCTCACCA CTCTAAATGC TCTAGAGATG GAACTAAGGA CATGTGGCCT ACCATATATT
    AATTTGGAAT TCTTAAAGGC TCACACCATG TATCAAGTGG GTCTAATGGA GACAGATCAA
    CACATTGAGT ATTTTTGGTC TGCCTTAGAA ATGTTCACTC AGGAAGAACT TTGCAAATTC
    ATAAAATTCG CCTGCAATCA GGAACGCATT CCCTTTACAT GTCCATGCAA AGATGGTGGG
    CCAGACACGG CTCATGTTCC ACCTTATCCT ATGAAAATAG CACCACCCGA TGGAGCAACA
    GGCTCCCCAG ATTCCAGGTA CATCCGAGTG GAGACATGTA TGTTCATGAT CAAGCTTCCG
    CAGTACTCGT CTCTAGAGAT GATGCTGGAG AAACTGAGAT ATGCCATTCA CTACAGGGAA
    GACCCACTCA GCGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31531
    Clone ID Related Accession (Same CDS sequence) XM_012968806.2
    Accession Version XM_012968806.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13128bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product probable E3 ubiquitin-protein ligase HECTD4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968806.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
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    1261
    1321
    1381
    1441
    1501
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    1801
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    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    ATGGGGTCGG CGGCTGCCGG GGACTCGGCT CAGTGGCTGT CGGTGAAAGA AGAGACTATC 
    TTTTTACATG ATGGGCTGAT CCGGGTCACA GACCTGGCGG AGTTGCCCAG CGAAATCGGG
    GTCAGTGAGG CCGGGGACGC AGAACTGGAG ATATTGACTT TTGAGACTAA GAACCCTATT
    GAGCTAGCAG AGCATTTACG TGCAGTGTGT GGGAACCAGA GCAATGCCTA TGCACGATTG
    CTGGAATACC GCTTGAATGC GTTACGTGGG TTATGGAATG CCCAACGCCA GCTGGCGCTT
    GAGGATCAAC ATGAAAGAGA TGGGGCTGAT GACGAAACCT TGGCCCTGCT TAAACGGCAG
    GGTTTGTTGC AACAGCCGGA ACAGGCTCCA TTTACATCTC GAATGGGGCT TTTACTGGTG
    TTCCCCCTTA TCCAGTCCCA AAGTAAAACA GACCCTTCAC TTTGTAACTT GACAGCTGAG
    GTGCTTTTAA ATTGCCTTCG GGACTGCCAA CCCCTGAGCT TGACTAAAGA ACCAGCTGAC
    TGCCTGAATG GAATAGAGTC CTTACTGTGT TCTTGGTTAG AAGACACTCC AAATGTGGGC
    CAACAAGTCT CCTTTAAGCA AAAGGAAAAT TCTGCTGCTG CTTTGGTTGC CTTAGCTTGT
    GCTAGGGGAT CATTAAAAAC ATTTATTCAC ACTGTACACT TGCTGCAAAA ACAGACTGAC
    CTAGGATCCC TTCCTGTGGC AGATGTATTG TATAGGTTGC TGCTTTTAGA GGGAGGTCCA
    GGTTCTCCCT CTTGTTTACT GGGAAGCAAA CACATTGTAT CCTGGGGTTT TGAGGATATG
    CTTCCAGCTC CTGATGGGAT CAGTGGTTCA AACAATGAGA ACAAAGATGC AGATTTGGGT
    CGCTGTGTAG CATGCGATGG GCTTTATCTT TACACAACTA ACTCTGTAGG TAGAGGATTG
    AGCAAAGTAG GGTCTGGGCT GCATGGCACT TTACGAGGTT TTGTTTATTC CCGAAACGAG
    GAGCTAGAGG CTGGCTGGAT TGCTTATGGA AGTGGGAAGA TACTTCACAG GCCTGCCTCC
    TTTGATAACA AACCTCAATA TCTTTTCCAA TTAATTGACC AATACACACT TCAGGTCTCT
    TATATAGTCA GCATGCCAGC CAATCATTTC CCGGTTGGCA GCAATATGAC TACGGTGCAC
    CTCTCCTCGG ATGGCACGTA TTTTTACTGG ATTTGGTCTC CAGCTAGTTT AAATGAGAAA
    ACCCCCAAAG GACACTCTGT CTTCATGGAT ATATTTGAGT TAAATGTGCA AAATGGAGTA
    TGCATTGCAA GCCCTCTTCA TGAGCGCATT ATATTAATGA GGAAAGAGGG AGAATCTGCT
    AAAAGTATTA ATGAAATGCT TTTGAGCAGA TTATCACGAT ACAGAGCTTC TCCTTCAGCA
    ACTCTAGCAG CACTTACAGG ATCTACAATC TCTAACACTC TAAAAGAGGA CCAGACTGCT
    GCAAATACGA GCTGCGGGCT TCCTTTGAAA ATGTTAAGAA AGACTCCAAT GTATACTTGT
    GGCACATACT TAGTACTACT CGTGCCACCT CCGGGTGGGT CAGGAAGTTC AGCAACAAGA
    TCTTTGTTTG GTGGAACCAG CGGCCTCTCC TCTTTAAAAA GTGCCTGCTA TGATACAATG
    AATAACATGA TCTGGACGTG CTCCAATGAT TACATCGATC AGTGGTGTAA TCCTGGAAAC
    CAGGCTTTCC ATGTGGTGTG TCAGAGGCTT GGTGTCACTC ACATCATTGC TGATCCCAAA
    GGAGAGGCAG TATCAACAAA TGAAGTTATA AACCAGTTGC TTCACCATGT GGGTGCTATG
    TGTATTCACC AACTCAATCT CCTGGCTAAT AGCAACAGTT CTCCAATCAG TACCTTTCTG
    GGCAAACAAC ATCCTATAGA GCCACATCAT CTTAATAGTA TATGTGACAT CATGGAGAAA
    GCCATGGTCA ATGGTGATAA ATGCATTATT CGCTGCATCT TGGTTGTTTT CCAGGTGGTA
    TTTAAATTCT TTCTAAACCC ACAAACTGAG AGGAATCGAG AAACCATAAG GAGGGCTGGT
    TTGCTCCTTT GGCAGTTGCT CATGGCGCCT AAGGACCAAA TTTGTCCTGA AATACAGAGA
    GAGGTCTGCT TGGCTATTAG CTTTGGTTTA AACATTTTGA ATCCAGGAGA GCTAGAAATT
    AATAACTTGC TCAAGCTGAT TTTAACTGAA GGGGACAGAA ATTGTGGCCT CTCCCAACTC
    CGTGATGTCA TTTTGACAAA CCTCACTGAA CAGCTTCAGA ATAACAGGTT TGGAAGTGAA
    GATGATGAAC ATTACAGGCT TAATGATGAA CTATTGCACT ATATTTTGAA GATTGTTGTT
    CGTGAATCCT GTGTTCTGAT CACCAAGTGC CAAACAGTGT CTAAAGAAGA ATTCCAAAGA
    CTCTTGTCAA CAGTGCCTGT GGCTTCTCCC TGTCTATGCT ATTTAATGGC TGTACAGAAT
    CATCTCCTGA GTAACACTGT CTTAATTAAA CCTGATGACA GCGATGACAG TGACAACTCC
    CTGCAGGGGG AGACATTGAA GGTACAGGAA CTCCAGGCCA GCATCTTGGC TTTGGCAACC
    CAGATACTTT CTGGTTGTGA TGAAGTTCTG GATATTCTGC AGCGAGTGAC CACTGCTCTT
    ATCAACAGCG ACCTGTTAGA CAGAGATTTA AGACTGAAAG GCCTTGAACA GATCACAAAG
    TCCACCCTGC TTGGCCATCT CCTCCCAGTA CTTCTCACAT CCCTTATGCA TCCAAACTTA
    CAGACATTAA CCATGGCAGA TGCGCTTATG CCTCAGCTGG TGCAGCTTGT TCTTTACACT
    AGCCAGACTG CACTGCTGCT TAAAACCCAG TGTCCAGTAT TGGCAGATAC AGGGGACACC
    ACCAGTGGAA TTCCAGAGCA AAAAAACAAA CTGTTAACTG ATGACAGGAT GCTGGAAGAA
    AAGGAGGAGC CTGGTTTCCT CACTGGGCTA AAGATTCCTG CGCCATGGGC TGCAGGAAAG
    ACTGTGGAAA CATCTCATCC AGTCAGAGAC AATTACAAGT TTAAAGAGAC TGTGCACATT
    CCTGGAGCTC GTTGTTTGTA CCTCCGGTTT GACAGCAGGT GCTCTTCACA GTACGATTAT
    GATAAGCTGG TGATATATGC GGGACCCAAC ACAAACAGCA GAAAAGTTGC CGAATATGGT
    GGAAATACCC TGGGGTATGG CAGTAGAAGT GTTTTAGGTA CAGGCTGGCC AAAAGATTTG
    GTTAAGGTGG AAGGGGATAC TGTGACATTC TCCTTCGAAA TGAGAAGTGG GCGTGAGCAC
    AACACTCCTG ATAAAGCAAT GTGGGGTTTT GCGTGCACTG TCAGAGCTCA GGAGTCCTCA
    GAAGATGTGT CAGGGGGCTT GCCATTTCTA GTAGACCTGG CTTTAGGTCT GTCTGTCTTG
    GCTTGTTCCA TGCTGCGTAT ATTGTACAAT GGTCCAGAAA TCACAAAAGA AGAGTTGACT
    TGTCACGATC TGTTGAGGTC AAAGCTTTTG CAGAGGTGTC AGTGGCAGGT GGAGGCTAAT
    GGTGCTTTAT CCCCGGCACT CACCCCAAGC CCTTCGCCGC TGCCCCTTAC TATAGAAGAA
    GACCAAGAAT TTACGTATCC ATCTGACATC TTGTTGCCAC CAGTTGGGAA CTATTTTGAA
    CTGCCAAGAA TCAGACTGCC TCCTGGTATT ATGATCAAGC TCAGGGAAAT ATCTGGACGC
    GCTCGACCAC AGTTTAGACC AAGCATAAAA GAGGTTATCC AGCCTGATGT GATGGAAGAA
    ATGGTTGTAT CTTGCGTCAT TAAACACTTG AACCTGGTCG ATGCATTGCA GTCTTTAATA
    AACTTCCAGT ATCGAGAAGA ACATCTAGAA GAATATGATT TATTATGTAA AATAATGGGG
    GAAACTTTTA AGAAGCTCAA TGCAATGGAA AGACAATTGC AGAGTGTTGC AGAACTGGAA
    CAAAAGTGGC AAAGTGAAGT GGAAGATGCT CAGAATGGAA AGCTGGAGAA CAACGCTCCC
    TTTTTTTACG ACTATCATTT TAATGAGAGC AAGTTAAAGG AACTTGAACT TTTATGTTCT
    ATGAAAGAGG TCCCTTCTGA TGGAAGTGAT CCAGAAAACA TGGTTTTCTC TCTGCGAGAG
    AAGTTTTTTC AAGAGGTGAA CTCCCTGATC CAAAAACCTT CCCACCCAAT GGCTAAGACC
    AAAACATTGG TTAAGAGTCT GATGAATAGA GCAGAGCTCC TGTTACATGT GACTATTGCA
    GCACATTCTG GTATCACGAG AAGCATATCT GGCACTCCAA CTGAACCTCA AGCTGGCAAG
    TCTATCTCAG AAGCAAAAGT TATATCTCAA GCCATGCGCC AGCCTGTTTT CCTCCGCAGC
    ATGTCTGCCC CTTCTGACCT GGAAATGATT GGAAGTAGTG ACTTAGAGTT CAGCAGGTCT
    AATCCAAGGA GAAGGAACAT CACTAGCCAC AGGAGCAGCT CCTTCACTCT GCTTCAGTCT
    TTAGCTATTG AAGACTGCAA GGATAAACCA ACATACAGTG TCCTTTTAGG GCAACTGTTT
    GCCTTCATTG GGACCAATCC CGACCAAGCA GTATCCAGTA GCAGCTTTCT ACTTGCAGCC
    CAAACCAGAT GGCGTCGTGG TAATACTCGC AAACAAGCCC TTGTACACAT GAGAGAATTA
    CTGACTGCTG CTGTCCGTGT TGGGGGTGTG ACACATTTGG TTGGACCAGT AACAATGGTG
    CTACAGGGAG GACCAAGAAT TGAAGAGCTC ACCTGTGGAG GAATGGTGGA GCTTGTTCAA
    GAGGCATTTG GAGAGACCAT GACCTCAGTG GTGTCACTTT GTGCACGGTA TCCCATTGCC
    TGTGCAAATA GCATTGGGCT TTTGTGTACT ATACCTTACA CAAGGAGTGA AGAAAAATGT
    TTAGTTCGCA GTGGGCTTGT TCAACTGATG GACCGACTCT GCAGCTTAAG CAACCAGAGA
    GAGTCCAGCT CAAATGAAAA GCAAACCAAG AAACAGAAAG TAGCAACTAT GGCATGGGCA
    GCCTTCCAAG TACTGGCCAA TAGATGTGTA GAATGGGAGA AAGAAGAAGG TGGTTCTACT
    GAGGCGGTAC ACTCTGGTTT GGCTAGACAA GTCTCCACCC TCCTCACTAA CCATCTAGCA
    AGGGCTACAG AGTGCTGTGG AAACCAAGCT GCAGGGAATG ATGCCCTACA GGACGTTCTG
    AGCCTTCTCA ATGATCTCTC TAGGAGTCAG ATAGGGAAGG CAATTCTGAG CCAGCCTGCT
    TGTGTGTCCA AACTTCTTTC GTTGCTGCTG GACCAGCGTC CTTCTCCAAA GCTTGTGCTC
    ATAATTCTGC AGCTGTGCCG CGCTGCACTT CCTCTCATGA GTGTTGAAGA CTGTGGAAAT
    GTAGAACTTC CTCCATGGAG TTACTCTTTA CCAGCTCTGG ACAGTGAACA GGGGGAGGAT
    CCAAGTGACC CAGCCTCCAA AATAGCATCT TTGTTGCTAG CAAAGTTGGC TGATTATGTT
    GTTCCAGGAT GTCAGACAGT TCTTTCTCCT ACTGCTTCTG AACCAGACAG CTCATTGTCC
    AAAAACAGCC CAAAAAATTC CCAGAAAGCA GATAAGGATG CAGAAGAAGA GAGTGAAGCA
    GTAGATGGCA AGCTCTCCAT TTTTATTCAT AAGCGAGAAG ACCAGTCATC CCATGAGGTT
    CTTCAGCCTT TGCTTAGTAG TTCAGAGGGA AGGCCTTTTC GTCTTGGTAC TGGAGCGAAT
    ATGGAAAAAG TAGTAAAGAT GGATCGTGAC ATGACTAAGA GTGGATGTTG TGAAGTAATC
    ACAGAGGAGG CTGCAGCTGC TCTCCGCAAA GCCACTAAAT GGGCCCAGTC GGGTTTAATT
    GTTAGCATTG GGCCACCTGT GGAGACAGTT AGCCCTGATT CAGCGAGTGG ACTTTCCACT
    GGTGACAAAA AGAAAACAGC CCAGACCTCG ATCTGTAGGG AAAGGAATTC TGAACTTGCC
    AGGACTGATC CTGTACGCCC TTTCATTAGT GGCCATGTAG CAAACAGCAT GGCTGCAGAA
    GTAATTGCTC TGCTTCACAG CCTGCTGATG GCACCAGAGT CAAATGCTGC CCAAATCTGG
    ACAACAACAG CAGAGAAGGT TCTTTCTCGT GCCCTGATGT ATATCCCACA GCTTGGCAAA
    TATGCAGAAA GCATCCTAGA GAACGGCAGC GGTAATGGCA GGAAGCTAGC AAAGCTACAA
    GGCATTGCAA GGCAAGCAGT TGCTGCATTA TGTGCGCTTG GTGGATTTAA AGAAACCATT
    AAAATAGGTT CTGAAGTCCA GGTGTTGGGT AAAGGAATAT CTGGGAGCAT TGGAGTGGTT
    GTTTCTATTA ATGAACAAGA AGGTATTGCA ACAGTGAAAT TTCCAGACAT AGATTTTAGA
    AAATCATGCA AAGCATCTGA CACACTGACA GTACCATTAT TTAGGCTCTG CGTTCCACGG
    TCAGAGGCAT TGCCTCTCCA TAAACTGTCC ATTACTGAGA AGGTAGTGCA GGCAGTCCAG
    TCCATGTTAC TTCCCCAGGA GGGCTGCCTA TCTATTCATA CCTCGCTTCC TGCAACAGGA
    GATGCCTCAA CTCCAGTAAT GGCAGTGGTT CGACTTCTTG CAGAGATCAG GACAAGAGCT
    TGCTTGGTGA TGGCTCAGCT GTTGGAGGAC AGCTCATTTT GTGAAGAATT CATACAGCAA
    TGTCCAGCTG CTGTAGAAGT TCTAAACCTA GTGGCCCAGG AATGCAGTCC TGGGGAGCGT
    CTCTCAGTGG TGGAAGTTCA GTGTGAAAGA CTACGAATGC TTTATCGGGA CTGTGCTCGC
    CCCCCGCCAC CACCTTTGCA GGCTGAGCGC AGACAGCCCA AGGAAATCAC CTGGTCTCCT
    TCCAGGGTAT TCCCTCCTGT GAGAGCCTGT ATGTTTTCAT CCCATCTAAC AGCAGTAACA
    TTTCTGGCTG ACCCAAGTGC TGGTGGGGGA CTTCCTCGCG GCACATTTAT ATATGCAACA
    TCCCCTGTTC CAGTACAGGC ACCATCTTTT TACTGGGAAA TAGAAGTAGT TTCCTATGGA
    GACACAGATG ATGACACTGG CCCTATTGTA TCATTTGGGT TTGCTACAGA AGCAGAGAAG
    CGGGATGGAG CATGGACAAA CCCAGTCGGT ACCTGTCTTT TTCATAACAA TGGTCGAGCT
    GTACATTACA ATGGATCAAG TCTCTTACAG TGGAAGAGTG TTCGATTAGA TGTGACTCTT
    GCTCCTGGTG ATGTTGCTGG AATAGGTTGG GAGAGAACTG AAGGAACACC ACCACCCCCA
    GGGCAGCCTG CCAAGGGCAG AGTCTACTTT ACTTACTGTG GACAGAGGCT TACTCCGTTC
    TTAGAGGATG TCTCTGGGGG CATGTGGCCA GTTGTGCATA TTCAGAAAAA GAACACTAAA
    GTCCGTGCAA ATTTTGGATG CAGACAGTTT GCTTTTGCCG AGGGTCAAGC TCATCGCAAT
    GCAGCAGATT TGTGCATTGA TTTGGCTGAA GAGATAAGTG CAAACTTTGA AGCATTGCCC
    TTTGCTATGG CATCTGACAG TGATAATGAT GCTGGGACAA GCATAGCATC TGATCCTGGA
    ACTCATGGAC CTCCTTGCCG TATTGCTGCA GTTTCTACTG TGCAACAGCA ATATGACAGT
    GATGCCTCAT GCCGTTACAA GGTAGAGCTG AGTTATCAAG ACTTTGTGAC CTCTGGACCT
    GATCCACACC CACCTCCCAT TGCAGATGAT GAAAGTGATG ATGATGACGA TGATGATATT
    CCTCGAGAGG ATCATTATGC TCTTTTGGTA AAAGCATGGG AGACCAAAGT TTTTCCAACC
    ATTCGAAGAC GTTTCCGTAA CGAGGCAGAG AGAAAGTCTG GCCTGGACCA GATTAAGGGA
    GCTCTTCAGC TAGGAATGGT GGATATAGCG AGGCAAACTG TAGAGTTCCT CTATGAAGAA
    AATGGTGGCA TTCCAAGAGA TCTCTATCTT CCTACTATTG AGGATATCAA GGATGAGGCA
    AATAAATTCA CCATTGATAA AGTTCGCAAA GGACTCACAG TGGTCATTCG ATCCCCAGAT
    AGCAACAATG TAGCTGGGAC AACTGTGGGA ACAGCTTTAC CCAAGTTTGC CATCCGTGGT
    ATGCTGAAGA CATTCTCTCT ACATGGGGTG GTTCTGGATG TGGATTCAGT TAATGAGCTG
    GTTCAGGTAG AGACTTACCT CCGGAGTGAA GGGGTATTAG TGAGATACTG GTATCCCATA
    GATATGCTAG AAAGACCTCC TGCGGGATAC AGGAGAATGG CAACCAATGG ACTGGTGACT
    TTAGATAGCA CTAACATCCA GATTCATAGA GAGCTGTTGC GGTGTGAGTC TGCACTTGCC
    AGGCTTTACT GTCGTGTGGC TTTGCTTAAC ATCTTTGCTC CTAAGTCTCC GCACACTTTC
    ACTCGCCTGT TCCATATTCC TGCTATCCGA GACATTACAT TGGAGCATTT ACAGCTTCTG
    TCAAATCAGC TTCTAATGCC ACCCCTTCCA GACGGAACCA TTACCTCCAG CTCTATCCTT
    CTTGCCCAGT CCTTGCAACA TTGCATCTAC TCCCAGAACT GCTCAACAAC AGATTTGTTC
    TACCAGGGCA ATGCACAGCC AATAAGAGAG TGGCTTGGTG TGGCCATAAC CAGGGCCCTG
    CATCAAGGAG AGGACAGTCT CTTGGATCTG ACAAAACAAA TCTGTTCCTT CTTGCAAAAT
    GCGCCGGAAC AGTTTCCATC TGAAGAATTT CCCATCTCTG AATCCAAGCT CAGTATGGAT
    GTTAATTTTC CTGGGGCAGC TTTTGTTATT GTTTCTTGTA TAGAGAGTCC GTCTGGATTT
    AGAAAAGACT CCTCCTTGTA CAAGGCTCCC TGGTCTAGAG TCATGGTATA TGGCCTTGGA
    CATAAAGTAA AGAGGAATGG ACAGCTGAAC CTGATAGAAT CAGTTTGTTA TCCACGTGAT
    GCCTCCCCAA GTAACACAGG CCTCACTCCC CCACCAACCA CAAACCAGTA CCCAACAGCG
    ATTATTCCCA CTGACAAGGT CCACGTCAAA CTTGGTGTAT CACCTACTCC TGGTGCTGTG
    CTGGTCATCC ATTCCCTGCC TCTAGAGTTT CCGTTGGCAA TGGCATTCAC AGAACAGCTG
    TTAACCTGGA AGTTGGAGGA CACAGAAGAG AAGTCAGAAG ATGAGCTTGA TACTGTTCCA
    TCCTCTGCTC TGTTGCAAGT GGTAGAACTT TTAGGTACTT TGATTTGGAC AACAGACCTT
    GCTGCAGTAA TTAAGGAGCT TCTGTTCCAT CTGTTGGCTG AACTATTAAG GAAAGTTCAC
    CATCTAGAGC AGAAGAAGAA CCCAAGTGGA TTATCTTCCT CGATAGCCCT GCAGTTGAAT
    CCATGTCTAG CAATGCTTAT GGCTTTACAG ACAGAACTGC GCAAGCTGTA TGATGAAGAA
    ACTCAAAACT GGGCATCTGG GAATGCATGT GGGGGGTCTG TACCTGGAGC CTCTGAGCAG
    GGAAGATTTT CAACTTACTT TCATGCCCTC ATGGAAGTGT GCCTGGCAGT GGCAGAAGTG
    ACGCTCCCAA TCAACATGAG CTCAGCAATC AGTGTGATGG CATCTACAAA TGCCACTAAT
    CTGAGTGACT CTTCTTCTTC CTCTTCCTCT TCCCCTGGAC AGACTCCTCA AAGTCCAAGT
    CTGTTATCAA AGAGAAAGAA AGTTAAGATC AAACGTGAGA AGACTGCATC TGGAAAACGT
    ACACCTTCAC GTGGTGCAGA GAGTGATGCT GCTTTGCTTA GCATTGGAGG AAGCAAACCT
    GAGGACATGT TGTGGTTCCA TAGGGCATTG ACTTTATTGA TGATCCTAAG GCATCTAACT
    AACAAGGATC CACAGGGGAT GGGTGTCACA AACGATGCTA TTGCTGATGC TAGCCAGGCT
    TTAGTTGGTC CCACAGCTCA CAGTCGTCTC CTTGTGATTT CTGGCATCCC TACACACCTA
    GAAGAATCAG TGGTCAGAAG TGCAATTAGA AAGGCATGTA ATGCTCATGG TGGAGTCTTT
    AAAGATGAAA TCTACATTCC ATTGCAGGAA ACTGAAACCC CTAAACCCAA AGACAGAATA
    GAAGGGGCAG AATGCAAGAC AGAAATAGAA AAACCCTCTA CGCAGCATAC AACCGACAAT
    ATGGATATCA GCAGCTCGAG CAGTGTAACT CCTGCAGCAA GTGTCACGGC ATCAGCTTCT
    ACAAGCCAAG CTTCATTGTG CAGCTCCCAA GGGGGGGTAT CACGGACCAC AAGTGAAACA
    TCTATGGAGC AGTTTGCCCC AGAACATGCT GTGCCATCAG GGCTTCTGGA ACCAACTATT
    ATCTCCAGCC AGGAGAGTCT GGATCTTTCT ATGTGCAGCA CAGGAAGCCT CGGCAGTCTG
    GGAAGTCTTG TTGAACAGCT TGATAATGTT GAAAACTCTT TGTCTGACGT GGGCTCCCTA
    TACACAGCTA CATCACTAGA TAGCCATATG GCAGAGTCGC GGCCAATCAA AGGATTTGCA
    GTTGTAGAGG TGCGATCACG AGCTAAAATA GAGAAAATCC GTGCCACTTT ATTTAACAGT
    AGTGACCTCA TTGGGTTATC TGGTTTGGAT GGTGAAGATG AGCTGATGGA AATGTCTACT
    GAGGAGATCT TGACTGTTTC AACAGTGAAT CAGAGCTTGT TTGATACCCA AGGAATACCC
    GGTCTGGCTG ATTATTTCAT GGATAAATCA ATGAAAGGTG ATAAATTAGT CCCGGAAGCT
    CGTGATGTTC TGACTGAGAT TTTCAAGAGC TGCATTCACG CCGAGCAGAT GCTGAGTCTG
    ACTCCAGCCA AGTTTATCAA AGTGTCTGAT ATCTACCTTA GCAAGGAGCA AATCAATTCA
    CAGACCCCAG GGAACTTACT GCATACCTTT TTCACCAATG TTCGACCACC TAAAAAAGTC
    CTGGAAGATC AACTAACGCA GATCTTAAGA AAATATGGAA TTCCCAAGCC TAAATTTGAC
    AAAAGCAAAT ACAGCAAAAC TGGGAAGGAG CAACATCCTG TGAAAGTTGT AAGCACCAAA
    AGACCTGTGG GCAAACCACC GACCAAAGAC AAGGCTGTAT TCAACAGCGT CAGGACTGCT
    TTAAGTGAGA AAAAACCAAC TGTGAAGCCA AAATCTCCGG AGAAGGGCAG GCCAGATGAG
    AAGGACCCAG ACAAATCTCC AACAAAGAAG CAAGAAAGGT TTCACTTGTG TCCCTATCTA
    GTTCCCGAGG AGAGATATCT GACGTTGGAT GGATTTCACC GCTTTGTAGT AGATCGGGCT
    AAGCAAGACA TTCGAAGCAT CTGGAGAGCT GTCTTGGCGT GTGGATATGA TCTTCATTTT
    GAAAGGTGTG CCTGCATTGA TACAAGGCAT GCTCAGAAAT CTAGCAGAAA ATGGACCTTG
    GAAATGGATG TTGCTCTTGT TCAGTATATC AACCGCCTTT GCCGTCACCT TGCCATAACG
    CCAGCCCGAC TACACCCTCA TGAGGTTTAT CTGGATCTTG CAGATACGGC TGACCCAAGA
    GTCTCCTGCC TGCTGAATGT ACCAATAGAA AGCCTGCGCC TCCGATTTGC TCTTCTTCAG
    TCCCTCAACA CTACTTTAGA GACCTTTTTC CTGCCCTTAG TGGAGCTACG GCAAACAGAC
    GTATACACAC ACAGCATCGC ATCCCTACTC CAGCTAGCTA AAGGGTTAAT CTTTTACGAT
    ACAAAAGTGA ATGTGATGAA CCGGGTATTG AACGCAACAG TGCAGAGGAC AGCAGATCAT
    GCAGCCCCTG AGATCACGCT GGACCCACTA GAAATTGTAG GAGGGGAAAT TCGTACCTCA
    GAAAACTCCT ACTTCTGCCA GGCAGCCCGA CAGTTGGCAT GCATCCCTTC CTCACAACTG
    TGTGTCAAAC TGGCAAGTGG AGGAGACCCC ACGTATGCAT TTAACATCCG CTTTACTGGA
    GAGGAGGTGC ATGGTACAAG TGGCTCATTC CGTCATTTCC TCTGGCAAGT GTGTAAAGAA
    CTACAGAGCT CCTCGCTGTC TCTTCTACTG CTCTGCCCCA GCTCTGCAGT TAATAAGAAT
    AAGGGAAAAT ATCTTCTAAC TCCCAGTCCC ATAACATATG CAGAAGAGCA GTTGCTCCAT
    TTCTTTGGCC AACTACTGGG AATAGCCATC AGAGCCGATG TTCCACTCCC TCTGGATCTA
    CTCCCCTGCT TCTGGAAGAT GCTTGTAGGA GAACCTTTGG ACCCAGAAAC TGATCTTTAT
    GAAGCAGATA TTCTCACCCA CAACTACATC AAGAAGTTTG AAAATGTAAA TGATGAGTCA
    GAGCTGGAAG CTCTTTGTGC TGAAATTGCC TCTCAGCATC TTCCTACCGA AAGTCCAGAT
    TCTCCTCATA AACCAGCCTG CAAATTTACA TACTTGACAA TGACTGGTGA AGAAGTGGAG
    CTGTGCCCTA AGGGAAGACA CATTTCTGTA GTCTGGGAAA ACAAAGATAT ATATGCAATG
    GGAATCCGGA ACCTTCGTAT ACGTGAGCTG AAGAACCAGG AGTGTATGAG CGCAGTAAGG
    GCTGGGCTGG GCTCCATTAT ACCTCTGCAG CTCCTCACCA CTCTAAATGC TCTAGAGATG
    GAACTAAGGA CATGTGGCCT ACCATATATT AATTTGGAAT TCTTAAAGGC TCACACCATG
    TATCAAGTGG GTCTAATGGA GACAGATCAA CACATTGAGT ATTTTTGGTC TGCCTTAGAA
    ATGTTCACTC AGGAAGAACT TTGCAAATTC ATAAAATTCG CCTGCAATCA GGAACGCATT
    CCCTTTACAT GTCCATGCAA AGATGGTGGG CCAGACACGG CTCATGTTCC ACCTTATCCT
    ATGAAAATAG CACCACCCGA TGGAGCAACA GGCTCCCCAG ATTCCAGGTA CATCCGAGTG
    GAGACATGTA TGTTCATGAT CAAGCTTCCG CAGTACTCGT CTCTAGAGAT GATGCTGGAG
    AAACTGAGAT ATGCCATTCA CTACAGGGAA GACCCACTCA GCGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012824260.1
    CDS323..13450
    Translation

    Target ORF information:

    RefSeq Version XM_012968806.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 4 (hectd4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012968806.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    ATGGGGTCGG CGGCTGCCGG GGACTCGGCT CAGTGGCTGT CGGTGAAAGA AGAGACTATC 
    TTTTTACATG ATGGGCTGAT CCGGGTCACA GACCTGGCGG AGTTGCCCAG CGAAATCGGG
    GTCAGTGAGG CCGGGGACGC AGAACTGGAG ATATTGACTT TTGAGACTAA GAACCCTATT
    GAGCTAGCAG AGCATTTACG TGCAGTGTGT GGGAACCAGA GCAATGCCTA TGCACGATTG
    CTGGAATACC GCTTGAATGC GTTACGTGGG TTATGGAATG CCCAACGCCA GCTGGCGCTT
    GAGGATCAAC ATGAAAGAGA TGGGGCTGAT GACGAAACCT TGGCCCTGCT TAAACGGCAG
    GGTTTGTTGC AACAGCCGGA ACAGGCTCCA TTTACATCTC GAATGGGGCT TTTACTGGTG
    TTCCCCCTTA TCCAGTCCCA AAGTAAAACA GACCCTTCAC TTTGTAACTT GACAGCTGAG
    GTGCTTTTAA ATTGCCTTCG GGACTGCCAA CCCCTGAGCT TGACTAAAGA ACCAGCTGAC
    TGCCTGAATG GAATAGAGTC CTTACTGTGT TCTTGGTTAG AAGACACTCC AAATGTGGGC
    CAACAAGTCT CCTTTAAGCA AAAGGAAAAT TCTGCTGCTG CTTTGGTTGC CTTAGCTTGT
    GCTAGGGGAT CATTAAAAAC ATTTATTCAC ACTGTACACT TGCTGCAAAA ACAGACTGAC
    CTAGGATCCC TTCCTGTGGC AGATGTATTG TATAGGTTGC TGCTTTTAGA GGGAGGTCCA
    GGTTCTCCCT CTTGTTTACT GGGAAGCAAA CACATTGTAT CCTGGGGTTT TGAGGATATG
    CTTCCAGCTC CTGATGGGAT CAGTGGTTCA AACAATGAGA ACAAAGATGC AGATTTGGGT
    CGCTGTGTAG CATGCGATGG GCTTTATCTT TACACAACTA ACTCTGTAGG TAGAGGATTG
    AGCAAAGTAG GGTCTGGGCT GCATGGCACT TTACGAGGTT TTGTTTATTC CCGAAACGAG
    GAGCTAGAGG CTGGCTGGAT TGCTTATGGA AGTGGGAAGA TACTTCACAG GCCTGCCTCC
    TTTGATAACA AACCTCAATA TCTTTTCCAA TTAATTGACC AATACACACT TCAGGTCTCT
    TATATAGTCA GCATGCCAGC CAATCATTTC CCGGTTGGCA GCAATATGAC TACGGTGCAC
    CTCTCCTCGG ATGGCACGTA TTTTTACTGG ATTTGGTCTC CAGCTAGTTT AAATGAGAAA
    ACCCCCAAAG GACACTCTGT CTTCATGGAT ATATTTGAGT TAAATGTGCA AAATGGAGTA
    TGCATTGCAA GCCCTCTTCA TGAGCGCATT ATATTAATGA GGAAAGAGGG AGAATCTGCT
    AAAAGTATTA ATGAAATGCT TTTGAGCAGA TTATCACGAT ACAGAGCTTC TCCTTCAGCA
    ACTCTAGCAG CACTTACAGG ATCTACAATC TCTAACACTC TAAAAGAGGA CCAGACTGCT
    GCAAATACGA GCTGCGGGCT TCCTTTGAAA ATGTTAAGAA AGACTCCAAT GTATACTTGT
    GGCACATACT TAGTACTACT CGTGCCACCT CCGGGTGGGT CAGGAAGTTC AGCAACAAGA
    TCTTTGTTTG GTGGAACCAG CGGCCTCTCC TCTTTAAAAA GTGCCTGCTA TGATACAATG
    AATAACATGA TCTGGACGTG CTCCAATGAT TACATCGATC AGTGGTGTAA TCCTGGAAAC
    CAGGCTTTCC ATGTGGTGTG TCAGAGGCTT GGTGTCACTC ACATCATTGC TGATCCCAAA
    GGAGAGGCAG TATCAACAAA TGAAGTTATA AACCAGTTGC TTCACCATGT GGGTGCTATG
    TGTATTCACC AACTCAATCT CCTGGCTAAT AGCAACAGTT CTCCAATCAG TACCTTTCTG
    GGCAAACAAC ATCCTATAGA GCCACATCAT CTTAATAGTA TATGTGACAT CATGGAGAAA
    GCCATGGTCA ATGGTGATAA ATGCATTATT CGCTGCATCT TGGTTGTTTT CCAGGTGGTA
    TTTAAATTCT TTCTAAACCC ACAAACTGAG AGGAATCGAG AAACCATAAG GAGGGCTGGT
    TTGCTCCTTT GGCAGTTGCT CATGGCGCCT AAGGACCAAA TTTGTCCTGA AATACAGAGA
    GAGGTCTGCT TGGCTATTAG CTTTGGTTTA AACATTTTGA ATCCAGGAGA GCTAGAAATT
    AATAACTTGC TCAAGCTGAT TTTAACTGAA GGGGACAGAA ATTGTGGCCT CTCCCAACTC
    CGTGATGTCA TTTTGACAAA CCTCACTGAA CAGCTTCAGA ATAACAGGTT TGGAAGTGAA
    GATGATGAAC ATTACAGGCT TAATGATGAA CTATTGCACT ATATTTTGAA GATTGTTGTT
    CGTGAATCCT GTGTTCTGAT CACCAAGTGC CAAACAGTGT CTAAAGAAGA ATTCCAAAGA
    CTCTTGTCAA CAGTGCCTGT GGCTTCTCCC TGTCTATGCT ATTTAATGGC TGTACAGAAT
    CATCTCCTGA GTAACACTGT CTTAATTAAA CCTGATGACA GCGATGACAG TGACAACTCC
    CTGCAGGGGG AGACATTGAA GGTACAGGAA CTCCAGGCCA GCATCTTGGC TTTGGCAACC
    CAGATACTTT CTGGTTGTGA TGAAGTTCTG GATATTCTGC AGCGAGTGAC CACTGCTCTT
    ATCAACAGCG ACCTGTTAGA CAGAGATTTA AGACTGAAAG GCCTTGAACA GATCACAAAG
    TCCACCCTGC TTGGCCATCT CCTCCCAGTA CTTCTCACAT CCCTTATGCA TCCAAACTTA
    CAGACATTAA CCATGGCAGA TGCGCTTATG CCTCAGCTGG TGCAGCTTGT TCTTTACACT
    AGCCAGACTG CACTGCTGCT TAAAACCCAG TGTCCAGTAT TGGCAGATAC AGGGGACACC
    ACCAGTGGAA TTCCAGAGCA AAAAAACAAA CTGTTAACTG ATGACAGGAT GCTGGAAGAA
    AAGGAGGAGC CTGGTTTCCT CACTGGGCTA AAGATTCCTG CGCCATGGGC TGCAGGAAAG
    ACTGTGGAAA CATCTCATCC AGTCAGAGAC AATTACAAGT TTAAAGAGAC TGTGCACATT
    CCTGGAGCTC GTTGTTTGTA CCTCCGGTTT GACAGCAGGT GCTCTTCACA GTACGATTAT
    GATAAGCTGG TGATATATGC GGGACCCAAC ACAAACAGCA GAAAAGTTGC CGAATATGGT
    GGAAATACCC TGGGGTATGG CAGTAGAAGT GTTTTAGGTA CAGGCTGGCC AAAAGATTTG
    GTTAAGGTGG AAGGGGATAC TGTGACATTC TCCTTCGAAA TGAGAAGTGG GCGTGAGCAC
    AACACTCCTG ATAAAGCAAT GTGGGGTTTT GCGTGCACTG TCAGAGCTCA GGAGTCCTCA
    GAAGATGTGT CAGGGGGCTT GCCATTTCTA GTAGACCTGG CTTTAGGTCT GTCTGTCTTG
    GCTTGTTCCA TGCTGCGTAT ATTGTACAAT GGTCCAGAAA TCACAAAAGA AGAGTTGACT
    TGTCACGATC TGTTGAGGTC AAAGCTTTTG CAGAGGTGTC AGTGGCAGGT GGAGGCTAAT
    GGTGCTTTAT CCCCGGCACT CACCCCAAGC CCTTCGCCGC TGCCCCTTAC TATAGAAGAA
    GACCAAGAAT TTACGTATCC ATCTGACATC TTGTTGCCAC CAGTTGGGAA CTATTTTGAA
    CTGCCAAGAA TCAGACTGCC TCCTGGTATT ATGATCAAGC TCAGGGAAAT ATCTGGACGC
    GCTCGACCAC AGTTTAGACC AAGCATAAAA GAGGTTATCC AGCCTGATGT GATGGAAGAA
    ATGGTTGTAT CTTGCGTCAT TAAACACTTG AACCTGGTCG ATGCATTGCA GTCTTTAATA
    AACTTCCAGT ATCGAGAAGA ACATCTAGAA GAATATGATT TATTATGTAA AATAATGGGG
    GAAACTTTTA AGAAGCTCAA TGCAATGGAA AGACAATTGC AGAGTGTTGC AGAACTGGAA
    CAAAAGTGGC AAAGTGAAGT GGAAGATGCT CAGAATGGAA AGCTGGAGAA CAACGCTCCC
    TTTTTTTACG ACTATCATTT TAATGAGAGC AAGTTAAAGG AACTTGAACT TTTATGTTCT
    ATGAAAGAGG TCCCTTCTGA TGGAAGTGAT CCAGAAAACA TGGTTTTCTC TCTGCGAGAG
    AAGTTTTTTC AAGAGGTGAA CTCCCTGATC CAAAAACCTT CCCACCCAAT GGCTAAGACC
    AAAACATTGG TTAAGAGTCT GATGAATAGA GCAGAGCTCC TGTTACATGT GACTATTGCA
    GCACATTCTG GTATCACGAG AAGCATATCT GGCACTCCAA CTGAACCTCA AGCTGGCAAG
    TCTATCTCAG AAGCAAAAGT TATATCTCAA GCCATGCGCC AGCCTGTTTT CCTCCGCAGC
    ATGTCTGCCC CTTCTGACCT GGAAATGATT GGAAGTAGTG ACTTAGAGTT CAGCAGGTCT
    AATCCAAGGA GAAGGAACAT CACTAGCCAC AGGAGCAGCT CCTTCACTCT GCTTCAGTCT
    TTAGCTATTG AAGACTGCAA GGATAAACCA ACATACAGTG TCCTTTTAGG GCAACTGTTT
    GCCTTCATTG GGACCAATCC CGACCAAGCA GTATCCAGTA GCAGCTTTCT ACTTGCAGCC
    CAAACCAGAT GGCGTCGTGG TAATACTCGC AAACAAGCCC TTGTACACAT GAGAGAATTA
    CTGACTGCTG CTGTCCGTGT TGGGGGTGTG ACACATTTGG TTGGACCAGT AACAATGGTG
    CTACAGGGAG GACCAAGAAT TGAAGAGCTC ACCTGTGGAG GAATGGTGGA GCTTGTTCAA
    GAGGCATTTG GAGAGACCAT GACCTCAGTG GTGTCACTTT GTGCACGGTA TCCCATTGCC
    TGTGCAAATA GCATTGGGCT TTTGTGTACT ATACCTTACA CAAGGAGTGA AGAAAAATGT
    TTAGTTCGCA GTGGGCTTGT TCAACTGATG GACCGACTCT GCAGCTTAAG CAACCAGAGA
    GAGTCCAGCT CAAATGAAAA GCAAACCAAG AAACAGAAAG TAGCAACTAT GGCATGGGCA
    GCCTTCCAAG TACTGGCCAA TAGATGTGTA GAATGGGAGA AAGAAGAAGG TGGTTCTACT
    GAGGCGGTAC ACTCTGGTTT GGCTAGACAA GTCTCCACCC TCCTCACTAA CCATCTAGCA
    AGGGCTACAG AGTGCTGTGG AAACCAAGCT GCAGGGAATG ATGCCCTACA GGACGTTCTG
    AGCCTTCTCA ATGATCTCTC TAGGAGTCAG ATAGGGAAGG CAATTCTGAG CCAGCCTGCT
    TGTGTGTCCA AACTTCTTTC GTTGCTGCTG GACCAGCGTC CTTCTCCAAA GCTTGTGCTC
    ATAATTCTGC AGCTGTGCCG CGCTGCACTT CCTCTCATGA GTGTTGAAGA CTGTGGAAAT
    GTAGAACTTC CTCCATGGAG TTACTCTTTA CCAGCTCTGG ACAGTGAACA GGGGGAGGAT
    CCAAGTGACC CAGCCTCCAA AATAGCATCT TTGTTGCTAG CAAAGTTGGC TGATTATGTT
    GTTCCAGGAT GTCAGACAGT TCTTTCTCCT ACTGCTTCTG AACCAGACAG CTCATTGTCC
    AAAAACAGCC CAAAAAATTC CCAGAAAGCA GATAAGGATG CAGAAGAAGA GAGTGAAGCA
    GTAGATGGCA AGCTCTCCAT TTTTATTCAT AAGCGAGAAG ACCAGTCATC CCATGAGGTT
    CTTCAGCCTT TGCTTAGTAG TTCAGAGGGA AGGCCTTTTC GTCTTGGTAC TGGAGCGAAT
    ATGGAAAAAG TAGTAAAGAT GGATCGTGAC ATGACTAAGA GTGGATGTTG TGAAGTAATC
    ACAGAGGAGG CTGCAGCTGC TCTCCGCAAA GCCACTAAAT GGGCCCAGTC GGGTTTAATT
    GTTAGCATTG GGCCACCTGT GGAGACAGTT AGCCCTGATT CAGCGAGTGG ACTTTCCACT
    GGTGACAAAA AGAAAACAGC CCAGACCTCG ATCTGTAGGG AAAGGAATTC TGAACTTGCC
    AGGACTGATC CTGTACGCCC TTTCATTAGT GGCCATGTAG CAAACAGCAT GGCTGCAGAA
    GTAATTGCTC TGCTTCACAG CCTGCTGATG GCACCAGAGT CAAATGCTGC CCAAATCTGG
    ACAACAACAG CAGAGAAGGT TCTTTCTCGT GCCCTGATGT ATATCCCACA GCTTGGCAAA
    TATGCAGAAA GCATCCTAGA GAACGGCAGC GGTAATGGCA GGAAGCTAGC AAAGCTACAA
    GGCATTGCAA GGCAAGCAGT TGCTGCATTA TGTGCGCTTG GTGGATTTAA AGAAACCATT
    AAAATAGGTT CTGAAGTCCA GGTGTTGGGT AAAGGAATAT CTGGGAGCAT TGGAGTGGTT
    GTTTCTATTA ATGAACAAGA AGGTATTGCA ACAGTGAAAT TTCCAGACAT AGATTTTAGA
    AAATCATGCA AAGCATCTGA CACACTGACA GTACCATTAT TTAGGCTCTG CGTTCCACGG
    TCAGAGGCAT TGCCTCTCCA TAAACTGTCC ATTACTGAGA AGGTAGTGCA GGCAGTCCAG
    TCCATGTTAC TTCCCCAGGA GGGCTGCCTA TCTATTCATA CCTCGCTTCC TGCAACAGGA
    GATGCCTCAA CTCCAGTAAT GGCAGTGGTT CGACTTCTTG CAGAGATCAG GACAAGAGCT
    TGCTTGGTGA TGGCTCAGCT GTTGGAGGAC AGCTCATTTT GTGAAGAATT CATACAGCAA
    TGTCCAGCTG CTGTAGAAGT TCTAAACCTA GTGGCCCAGG AATGCAGTCC TGGGGAGCGT
    CTCTCAGTGG TGGAAGTTCA GTGTGAAAGA CTACGAATGC TTTATCGGGA CTGTGCTCGC
    CCCCCGCCAC CACCTTTGCA GGCTGAGCGC AGACAGCCCA AGGAAATCAC CTGGTCTCCT
    TCCAGGGTAT TCCCTCCTGT GAGAGCCTGT ATGTTTTCAT CCCATCTAAC AGCAGTAACA
    TTTCTGGCTG ACCCAAGTGC TGGTGGGGGA CTTCCTCGCG GCACATTTAT ATATGCAACA
    TCCCCTGTTC CAGTACAGGC ACCATCTTTT TACTGGGAAA TAGAAGTAGT TTCCTATGGA
    GACACAGATG ATGACACTGG CCCTATTGTA TCATTTGGGT TTGCTACAGA AGCAGAGAAG
    CGGGATGGAG CATGGACAAA CCCAGTCGGT ACCTGTCTTT TTCATAACAA TGGTCGAGCT
    GTACATTACA ATGGATCAAG TCTCTTACAG TGGAAGAGTG TTCGATTAGA TGTGACTCTT
    GCTCCTGGTG ATGTTGCTGG AATAGGTTGG GAGAGAACTG AAGGAACACC ACCACCCCCA
    GGGCAGCCTG CCAAGGGCAG AGTCTACTTT ACTTACTGTG GACAGAGGCT TACTCCGTTC
    TTAGAGGATG TCTCTGGGGG CATGTGGCCA GTTGTGCATA TTCAGAAAAA GAACACTAAA
    GTCCGTGCAA ATTTTGGATG CAGACAGTTT GCTTTTGCCG AGGGTCAAGC TCATCGCAAT
    GCAGCAGATT TGTGCATTGA TTTGGCTGAA GAGATAAGTG CAAACTTTGA AGCATTGCCC
    TTTGCTATGG CATCTGACAG TGATAATGAT GCTGGGACAA GCATAGCATC TGATCCTGGA
    ACTCATGGAC CTCCTTGCCG TATTGCTGCA GTTTCTACTG TGCAACAGCA ATATGACAGT
    GATGCCTCAT GCCGTTACAA GGTAGAGCTG AGTTATCAAG ACTTTGTGAC CTCTGGACCT
    GATCCACACC CACCTCCCAT TGCAGATGAT GAAAGTGATG ATGATGACGA TGATGATATT
    CCTCGAGAGG ATCATTATGC TCTTTTGGTA AAAGCATGGG AGACCAAAGT TTTTCCAACC
    ATTCGAAGAC GTTTCCGTAA CGAGGCAGAG AGAAAGTCTG GCCTGGACCA GATTAAGGGA
    GCTCTTCAGC TAGGAATGGT GGATATAGCG AGGCAAACTG TAGAGTTCCT CTATGAAGAA
    AATGGTGGCA TTCCAAGAGA TCTCTATCTT CCTACTATTG AGGATATCAA GGATGAGGCA
    AATAAATTCA CCATTGATAA AGTTCGCAAA GGACTCACAG TGGTCATTCG ATCCCCAGAT
    AGCAACAATG TAGCTGGGAC AACTGTGGGA ACAGCTTTAC CCAAGTTTGC CATCCGTGGT
    ATGCTGAAGA CATTCTCTCT ACATGGGGTG GTTCTGGATG TGGATTCAGT TAATGAGCTG
    GTTCAGGTAG AGACTTACCT CCGGAGTGAA GGGGTATTAG TGAGATACTG GTATCCCATA
    GATATGCTAG AAAGACCTCC TGCGGGATAC AGGAGAATGG CAACCAATGG ACTGGTGACT
    TTAGATAGCA CTAACATCCA GATTCATAGA GAGCTGTTGC GGTGTGAGTC TGCACTTGCC
    AGGCTTTACT GTCGTGTGGC TTTGCTTAAC ATCTTTGCTC CTAAGTCTCC GCACACTTTC
    ACTCGCCTGT TCCATATTCC TGCTATCCGA GACATTACAT TGGAGCATTT ACAGCTTCTG
    TCAAATCAGC TTCTAATGCC ACCCCTTCCA GACGGAACCA TTACCTCCAG CTCTATCCTT
    CTTGCCCAGT CCTTGCAACA TTGCATCTAC TCCCAGAACT GCTCAACAAC AGATTTGTTC
    TACCAGGGCA ATGCACAGCC AATAAGAGAG TGGCTTGGTG TGGCCATAAC CAGGGCCCTG
    CATCAAGGAG AGGACAGTCT CTTGGATCTG ACAAAACAAA TCTGTTCCTT CTTGCAAAAT
    GCGCCGGAAC AGTTTCCATC TGAAGAATTT CCCATCTCTG AATCCAAGCT CAGTATGGAT
    GTTAATTTTC CTGGGGCAGC TTTTGTTATT GTTTCTTGTA TAGAGAGTCC GTCTGGATTT
    AGAAAAGACT CCTCCTTGTA CAAGGCTCCC TGGTCTAGAG TCATGGTATA TGGCCTTGGA
    CATAAAGTAA AGAGGAATGG ACAGCTGAAC CTGATAGAAT CAGTTTGTTA TCCACGTGAT
    GCCTCCCCAA GTAACACAGG CCTCACTCCC CCACCAACCA CAAACCAGTA CCCAACAGCG
    ATTATTCCCA CTGACAAGGT CCACGTCAAA CTTGGTGTAT CACCTACTCC TGGTGCTGTG
    CTGGTCATCC ATTCCCTGCC TCTAGAGTTT CCGTTGGCAA TGGCATTCAC AGAACAGCTG
    TTAACCTGGA AGTTGGAGGA CACAGAAGAG AAGTCAGAAG ATGAGCTTGA TACTGTTCCA
    TCCTCTGCTC TGTTGCAAGT GGTAGAACTT TTAGGTACTT TGATTTGGAC AACAGACCTT
    GCTGCAGTAA TTAAGGAGCT TCTGTTCCAT CTGTTGGCTG AACTATTAAG GAAAGTTCAC
    CATCTAGAGC AGAAGAAGAA CCCAAGTGGA TTATCTTCCT CGATAGCCCT GCAGTTGAAT
    CCATGTCTAG CAATGCTTAT GGCTTTACAG ACAGAACTGC GCAAGCTGTA TGATGAAGAA
    ACTCAAAACT GGGCATCTGG GAATGCATGT GGGGGGTCTG TACCTGGAGC CTCTGAGCAG
    GGAAGATTTT CAACTTACTT TCATGCCCTC ATGGAAGTGT GCCTGGCAGT GGCAGAAGTG
    ACGCTCCCAA TCAACATGAG CTCAGCAATC AGTGTGATGG CATCTACAAA TGCCACTAAT
    CTGAGTGACT CTTCTTCTTC CTCTTCCTCT TCCCCTGGAC AGACTCCTCA AAGTCCAAGT
    CTGTTATCAA AGAGAAAGAA AGTTAAGATC AAACGTGAGA AGACTGCATC TGGAAAACGT
    ACACCTTCAC GTGGTGCAGA GAGTGATGCT GCTTTGCTTA GCATTGGAGG AAGCAAACCT
    GAGGACATGT TGTGGTTCCA TAGGGCATTG ACTTTATTGA TGATCCTAAG GCATCTAACT
    AACAAGGATC CACAGGGGAT GGGTGTCACA AACGATGCTA TTGCTGATGC TAGCCAGGCT
    TTAGTTGGTC CCACAGCTCA CAGTCGTCTC CTTGTGATTT CTGGCATCCC TACACACCTA
    GAAGAATCAG TGGTCAGAAG TGCAATTAGA AAGGCATGTA ATGCTCATGG TGGAGTCTTT
    AAAGATGAAA TCTACATTCC ATTGCAGGAA ACTGAAACCC CTAAACCCAA AGACAGAATA
    GAAGGGGCAG AATGCAAGAC AGAAATAGAA AAACCCTCTA CGCAGCATAC AACCGACAAT
    ATGGATATCA GCAGCTCGAG CAGTGTAACT CCTGCAGCAA GTGTCACGGC ATCAGCTTCT
    ACAAGCCAAG CTTCATTGTG CAGCTCCCAA GGGGGGGTAT CACGGACCAC AAGTGAAACA
    TCTATGGAGC AGTTTGCCCC AGAACATGCT GTGCCATCAG GGCTTCTGGA ACCAACTATT
    ATCTCCAGCC AGGAGAGTCT GGATCTTTCT ATGTGCAGCA CAGGAAGCCT CGGCAGTCTG
    GGAAGTCTTG TTGAACAGCT TGATAATGTT GAAAACTCTT TGTCTGACGT GGGCTCCCTA
    TACACAGCTA CATCACTAGA TAGCCATATG GCAGAGTCGC GGCCAATCAA AGGATTTGCA
    GTTGTAGAGG TGCGATCACG AGCTAAAATA GAGAAAATCC GTGCCACTTT ATTTAACAGT
    AGTGACCTCA TTGGGTTATC TGGTTTGGAT GGTGAAGATG AGCTGATGGA AATGTCTACT
    GAGGAGATCT TGACTGTTTC AACAGTGAAT CAGAGCTTGT TTGATACCCA AGGAATACCC
    GGTCTGGCTG ATTATTTCAT GGATAAATCA ATGAAAGGTG ATAAATTAGT CCCGGAAGCT
    CGTGATGTTC TGACTGAGAT TTTCAAGAGC TGCATTCACG CCGAGCAGAT GCTGAGTCTG
    ACTCCAGCCA AGTTTATCAA AGTGTCTGAT ATCTACCTTA GCAAGGAGCA AATCAATTCA
    CAGACCCCAG GGAACTTACT GCATACCTTT TTCACCAATG TTCGACCACC TAAAAAAGTC
    CTGGAAGATC AACTAACGCA GATCTTAAGA AAATATGGAA TTCCCAAGCC TAAATTTGAC
    AAAAGCAAAT ACAGCAAAAC TGGGAAGGAG CAACATCCTG TGAAAGTTGT AAGCACCAAA
    AGACCTGTGG GCAAACCACC GACCAAAGAC AAGGCTGTAT TCAACAGCGT CAGGACTGCT
    TTAAGTGAGA AAAAACCAAC TGTGAAGCCA AAATCTCCGG AGAAGGGCAG GCCAGATGAG
    AAGGACCCAG ACAAATCTCC AACAAAGAAG CAAGAAAGGT TTCACTTGTG TCCCTATCTA
    GTTCCCGAGG AGAGATATCT GACGTTGGAT GGATTTCACC GCTTTGTAGT AGATCGGGCT
    AAGCAAGACA TTCGAAGCAT CTGGAGAGCT GTCTTGGCGT GTGGATATGA TCTTCATTTT
    GAAAGGTGTG CCTGCATTGA TACAAGGCAT GCTCAGAAAT CTAGCAGAAA ATGGACCTTG
    GAAATGGATG TTGCTCTTGT TCAGTATATC AACCGCCTTT GCCGTCACCT TGCCATAACG
    CCAGCCCGAC TACACCCTCA TGAGGTTTAT CTGGATCTTG CAGATACGGC TGACCCAAGA
    GTCTCCTGCC TGCTGAATGT ACCAATAGAA AGCCTGCGCC TCCGATTTGC TCTTCTTCAG
    TCCCTCAACA CTACTTTAGA GACCTTTTTC CTGCCCTTAG TGGAGCTACG GCAAACAGAC
    GTATACACAC ACAGCATCGC ATCCCTACTC CAGCTAGCTA AAGGGTTAAT CTTTTACGAT
    ACAAAAGTGA ATGTGATGAA CCGGGTATTG AACGCAACAG TGCAGAGGAC AGCAGATCAT
    GCAGCCCCTG AGATCACGCT GGACCCACTA GAAATTGTAG GAGGGGAAAT TCGTACCTCA
    GAAAACTCCT ACTTCTGCCA GGCAGCCCGA CAGTTGGCAT GCATCCCTTC CTCACAACTG
    TGTGTCAAAC TGGCAAGTGG AGGAGACCCC ACGTATGCAT TTAACATCCG CTTTACTGGA
    GAGGAGGTGC ATGGTACAAG TGGCTCATTC CGTCATTTCC TCTGGCAAGT GTGTAAAGAA
    CTACAGAGCT CCTCGCTGTC TCTTCTACTG CTCTGCCCCA GCTCTGCAGT TAATAAGAAT
    AAGGGAAAAT ATCTTCTAAC TCCCAGTCCC ATAACATATG CAGAAGAGCA GTTGCTCCAT
    TTCTTTGGCC AACTACTGGG AATAGCCATC AGAGCCGATG TTCCACTCCC TCTGGATCTA
    CTCCCCTGCT TCTGGAAGAT GCTTGTAGGA GAACCTTTGG ACCCAGAAAC TGATCTTTAT
    GAAGCAGATA TTCTCACCCA CAACTACATC AAGAAGTTTG AAAATGTAAA TGATGAGTCA
    GAGCTGGAAG CTCTTTGTGC TGAAATTGCC TCTCAGCATC TTCCTACCGA AAGTCCAGAT
    TCTCCTCATA AACCAGCCTG CAAATTTACA TACTTGACAA TGACTGGTGA AGAAGTGGAG
    CTGTGCCCTA AGGGAAGACA CATTTCTGTA GTCTGGGAAA ACAAAGATAT ATATGCAATG
    GGAATCCGGA ACCTTCGTAT ACGTGAGCTG AAGAACCAGG AGTGTATGAG CGCAGTAAGG
    GCTGGGCTGG GCTCCATTAT ACCTCTGCAG CTCCTCACCA CTCTAAATGC TCTAGAGATG
    GAACTAAGGA CATGTGGCCT ACCATATATT AATTTGGAAT TCTTAAAGGC TCACACCATG
    TATCAAGTGG GTCTAATGGA GACAGATCAA CACATTGAGT ATTTTTGGTC TGCCTTAGAA
    ATGTTCACTC AGGAAGAACT TTGCAAATTC ATAAAATTCG CCTGCAATCA GGAACGCATT
    CCCTTTACAT GTCCATGCAA AGATGGTGGG CCAGACACGG CTCATGTTCC ACCTTATCCT
    ATGAAAATAG CACCACCCGA TGGAGCAACA GGCTCCCCAG ATTCCAGGTA CATCCGAGTG
    GAGACATGTA TGTTCATGAT CAAGCTTCCG CAGTACTCGT CTCTAGAGAT GATGCTGGAG
    AAACTGAGAT ATGCCATTCA CTACAGGGAA GACCCACTCA GCGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa49024
    Clone ID Related Accession (Same CDS sequence) XM_012968793.3
    Accession Version XM_012968793.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13242bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product probable E3 ubiquitin-protein ligase HECTD4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012968793.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
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    301
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
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    3601
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    3721
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    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
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    4621
    4681
    4741
    4801
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    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
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    7621
    7681
    7741
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    7921
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    8401
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    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGGGGTCGG CGGCTGCCGG GGACTCGGCT CAGTGGCTGT CGGTGAAAGA AGAGACTATC 
    TTTTTACATG ATGGGCTGAT CCGGGTCACA GACCTGGCGG AGTTGCCCAG CGAAATCGGG
    GTCAGTGAGG CCGGGGACGC AGAACTGGAG ATATTGACTT TTGAGACTAA GAACCCTATT
    GAGCTAGCAG AGCATTTACG TGCAGTGTGT GGGAACCAGA GCAATGCCTA TGCACGATTG
    CTGGAATACC GCTTGAATGC GTTACGTGGG TTATGGAATG CCCAACGCCA GCTGGCGCTT
    GAGGATCAAC ATGAAAGAGA TGGGGCTGAT GACGAAACCT TGGCCCTGCT TAAACGGCAG
    GGTTTGTTGC AACAGCCGGA ACAGGCTCCA TTTACATCTC GAATGGGGCT TTTACTGGTG
    TTCCCCCTTA TCCAGTCCCA AAGTAAAACA GACCCTTCAC TTTGTAACTT GACAGCTGAG
    GTGCTTTTAA ATTGCCTTCG GGACTGCCAA CCCCTGAGCT TGACTAAAGA ACCAGCTGAC
    TGCCTGAATG GAATAGAGTC CTTACTGTGT TCTTGGTTAG AAGACACTCC AAATGTGGGC
    CAACAAGTCT CCTTTAAGCA AAAGGAAAAT TCTGCTGCTG CTTTGGTTGC CTTAGCTTGT
    GCTAGGGGAT CATTAAAAAC ATTTATTCAC ACTGTACACT TGCTGCAAAA ACAGACTGAC
    CTAGGATCCC TTCCTGTGGC AGATGTATTG TATAGGTTGC TGCTTTTAGA GGGAGGTCCA
    GGTTCTCCCT CTTGTTTACT GGGAAGCAAA CACATTGTAT CCTGGGGTTT TGAGGATATG
    CTTCCAGCTC CTGATGGGAT CAGTGGTTCA AACAATGAGA ACAAAGATGC AGATTTGGGT
    CGCTGTGTAG CATGCGATGG GCTTTATCTT TACACAACTA ACTCTGTAGG TAGAGGATTG
    AGCAAAGTAG GGTCTGGGCT GCATGGCACT TTACGAGGTT TTGTTTATTC CCGAAACGAG
    GAGCTAGAGG CTGGCTGGAT TGCTTATGGA AGTGGGAAGA TACTTCACAG GCCTGCCTCC
    TTTGATAACA AACCTCAATA TCTTTTCCAA TTAATTGACC AATACACACT TCAGGTCTCT
    TATATAGTCA GCATGCCAGC CAATCATTTC CCGGTTGGCA GCAATATGAC TACGGTGCAC
    CTCTCCTCGG ATGGCACGTA TTTTTACTGG ATTTGGTCTC CAGCTAGTTT AAATGAGAAA
    ACCCCCAAAG GACACTCTGT CTTCATGGAT ATATTTGAGT TAAATGTGCA AAATGGAGTA
    TGCATTGCAA GCCCTCTTCA TGAGCGCATT ATATTAATGA GGAAAGAGGG AGAATCTGCT
    AAAAGTATTA ATGAAATGCT TTTGAGCAGA TTATCACGAT ACAGAGCTTC TCCTTCAGCA
    ACTCTAGCAG CACTTACAGG ATCTACAATC TCTAACACTC TAAAAGAGGA CCAGACTGCT
    GCAAATACGA GCTGCGGGCT TCCTTTGAAA ATGTTAAGAA AGACTCCAAT GTATACTTGT
    GGCACATACT TAGTACTACT CGTGCCACCT CCGGGTGGGT CAGGAAGTTC AGCAACAAGA
    TCTTTGTTTG GTGGAACCAG CGGCCTCTCC TCTTTAAAAA TTCTGGCTTC CAGCTTGGTA
    TTTAACATTT CTGATGGGCA GTTCACCAAC AGAGCAGATC TTATTGATGC ATCTGGAAGT
    TCTCTTGGAA GGGGTGCTTT GGTGACAGGC CTAGGTGCCT GCTATGATAC AATGAATAAC
    ATGATCTGGA CGTGCTCCAA TGATTACATC GATCAGTGGT GTAATCCTGG AAACCAGGCT
    TTCCATGTGG TGTGTCAGAG GCTTGGTGTC ACTCACATCA TTGCTGATCC CAAAGGAGAG
    GCAGTATCAA CAAATGAAGT TATAAACCAG TTGCTTCACC ATGTGGGTGC TATGTGTATT
    CACCAACTCA ATCTCCTGGC TAATAGCAAC AGTTCTCCAA TCAGTACCTT TCTGGGCAAA
    CAACATCCTA TAGAGCCACA TCATCTTAAT AGTATATGTG ACATCATGGA GAAAGCCATG
    GTCAATGGTG ATAAATGCAT TATTCGCTGC ATCTTGGTTG TTTTCCAGGT GGTATTTAAA
    TTCTTTCTAA ACCCACAAAC TGAGAGGAAT CGAGAAACCA TAAGGAGGGC TGGTTTGCTC
    CTTTGGCAGT TGCTCATGGC GCCTAAGGAC CAAATTTGTC CTGAAATACA GAGAGAGGTC
    TGCTTGGCTA TTAGCTTTGG TTTAAACATT TTGAATCCAG GAGAGCTAGA AATTAATAAC
    TTGCTCAAGC TGATTTTAAC TGAAGGGGAC AGAAATTGTG GCCTCTCCCA ACTCCGTGAT
    GTCATTTTGA CAAACCTCAC TGAACAGCTT CAGAATAACA GGTTTGGAAG TGAAGATGAT
    GAACATTACA GGCTTAATGA TGAACTATTG CACTATATTT TGAAGATTGT TGTTCGTGAA
    TCCTGTGTTC TGATCACCAA GTGCCAAACA GTGTCTAAAG AAGAATTCCA AAGACTCTTG
    TCAACAGTGC CTGTGGCTTC TCCCTGTCTA TGCTATTTAA TGGCTGTACA GAATCATCTC
    CTGAGTAACA CTGTCTTAAT TAAACCTGAT GACAGCGATG ACAGTGACAA CTCCCTGCAG
    GGGGAGACAT TGAAGGTACA GGAACTCCAG GCCAGCATCT TGGCTTTGGC AACCCAGATA
    CTTTCTGGTT GTGATGAAGT TCTGGATATT CTGCAGCGAG TGACCACTGC TCTTATCAAC
    AGCGACCTGT TAGACAGAGA TTTAAGACTG AAAGGCCTTG AACAGATCAC AAAGTCCACC
    CTGCTTGGCC ATCTCCTCCC AGTACTTCTC ACATCCCTTA TGCATCCAAA CTTACAGACA
    TTAACCATGG CAGATGCGCT TATGCCTCAG CTGGTGCAGC TTGTTCTTTA CACTAGCCAG
    ACTGCACTGC TGCTTAAAAC CCAGTGTCCA GTATTGGCAG ATACAGGGGA CACCACCAGT
    GGAATTCCAG AGCAAAAAAA CAAACTGTTA ACTGATGACA GGATGCTGGA AGAAAAGGAG
    GAGCCTGGTT TCCTCACTGG GCTAAAGATT CCTGCGCCAT GGGCTGCAGG AAAGACTGTG
    GAAACATCTC ATCCAGTCAG AGACAATTAC AAGTTTAAAG AGACTGTGCA CATTCCTGGA
    GCTCGTTGTT TGTACCTCCG GTTTGACAGC AGGTGCTCTT CACAGTACGA TTATGATAAG
    CTGGTGATAT ATGCGGGACC CAACACAAAC AGCAGAAAAG TTGCCGAATA TGGTGGAAAT
    ACCCTGGGGT ATGGCAGTAG AAGTGTTTTA GGTACAGGCT GGCCAAAAGA TTTGGTTAAG
    GTGGAAGGTG ATACTGTGAC ATTCTCCTTC GAAATGAGAA GTGGGCGTGA GCACAACACT
    CCTGATAAAG CAATGTGGGG TTTTGCGTGC ACTGTCAGAG CTCAGGAGTC CTCAGAAGAT
    GTGTCAGGGG GCTTGCCATT TCTAGTAGAC CTGGCTTTAG GTCTGTCTGT CTTGGCTTGT
    TCCATGCTGC GTATATTGTA CAATGGTCCA GAAATCACAA AAGAAGAGTT GACTTGTCAC
    GATCTGTTGA GGTCAAAGCT TTTGCAGAGG TGTCAGTGGC AGGTGGAGGC TAATGGTGCT
    TTATCCCCGG CACTCACCCC AAGCCCTTCG CCGCTGCCCC TTACTATAGA AGAAGACCAA
    GAATTTACGT ATCCATCTGA CATCTTGTTG CCACCAGTTG GGAACTATTT TGAACTGCCA
    AGAATCAGAC TGCCTCCTGG TATTATGATC AAGCTCAGGG AAATATCTGG ACGCGCTCGA
    CCACAGTTTA GACCAAGCAT AAAAGAGGTT ATCCAGCCTG ATGTGATGGA AGAAATGGTT
    GTATCTTGCG TCATTAAACA CTTGAACCTG GTCGATGCAT TGCAGTCTTT AATAAACTTC
    CAGTATCGAG AAGAACATCT AGAAGAATAT GATTTATTAT GTAAAATAAT GGGGGAAACT
    TTTAAGAAGC TCAATGCAAT GGAAAGACAA TTGCAGAGTG TTGCAGAACT GGAACAAAAG
    TGGCAAAGTG AAGTGGAAGA TGCTCAGAAT GGAAAGCTGG AGAACAACGC TCCCTTTTTT
    TACGACTATC ATTTTAATGA GAGCAAGTTA AAGGAACTTG AACTTTTATG TTCTATGAAA
    GAGGTCCCTT CTGATGGAAG TGATCCAGAA AACATGGTTT TCTCTCTGCG AGAGAAGTTT
    TTTCAAGAGG TGAACTCCCT GATCCAAAAA CCTTCCCACC CAATGGCTAA GACCAAAACA
    TTGGTTAAGA GTCTGATGAA TAGAGCAGAG CTCCTGTTAC ATGTGACTAT TGCAGCACAT
    TCTGGTATCA CGAGAAGCAT ATCTGGCACT CCAACTGAAC CTCAAGCTGG CAAGTCTATC
    TCAGAAGCAA AAGTTATATC TCAAGCCATG CGCCAGCCTG TTTTCCTCCG CAGCATGTCT
    GCCCCTTCTG ACCTGGAAAT GATTGGAAGT AGTGACTTAG AGTTCAGCAG GTCTAATCCA
    AGGAGAAGGA ACATCACTAG CCACAGGAGC AGCTCCTTCA CTCTGCTTCA GTCTTTAGCT
    ATTGAAGACT GCAAGGATAA ACCAACATAC AGTGTCCTTT TAGGGCAACT GTTTGCCTTC
    ATTGGGACCA ATCCCGACCA AGCAGTATCC AGTAGCAGCT TTCTACTTGC AGCCCAAACC
    AGATGGCGTC GTGGTAATAC TCGCAAACAA GCCCTTGTAC ACATGAGAGA ATTACTGACT
    GCTGCTGTCC GTGTTGGGGG TGTGACACAT TTGGTTGGAC CAGTAACAAT GGTGCTACAG
    GGAGGACCAA GAATTGAAGA GCTCACCTGT GGAGGAATGG TGGAGCTTGT TCAAGAGGCA
    TTTGGAGAGA CCATGACCTC AGTGGTGTCA CTTTGTGCAC GGTATCCCAT TGCCTGTGCA
    AATAGCATTG GGCTTTTGTG TACTATACCT TACACAAGGA GTGAAGAAAA ATGTTTAGTT
    CGCAGTGGGC TTGTTCAACT GATGGACCGA CTCTGCAGCT TAAGCAACCA GAGAGAGTCC
    AGCTCAAATG AAAAGCAAAC CAAGAAACAG AAAGTAGCAA CTATGGCATG GGCAGCCTTC
    CAAGTACTGG CCAATAGATG TGTAGAATGG GAGAAAGAAG AAGGTGGTTC TACTGAGGCG
    GTACACTCTG GTTTGGCTAG ACAAGTCTCC ACCCTCCTCA CTAACCATCT AGCAAGGGCT
    ACAGAGTGCT GTGGAAACCA AGCTGCAGGG AATGATGCCC TACAGGACGT TCTGAGCCTT
    CTCAATGATC TCTCTAGGAG TCAGATAGGG AAGGCAATTC TGAGCCAGCC TGCTTGTGTG
    TCCAAACTTC TTTCGTTGCT GCTGGACCAG CGTCCTTCTC CAAAGCTTGT GCTCATAATT
    CTGCAGCTGT GCCGCGCTGC ACTTCCTCTC ATGAGTGTTG AAGACTGTGG AAATGTAGAA
    CTTCCTCCAT GGAGTTACTC TTTACCAGCT CTGGACAGTG AACAGGGGGA GGATCCAAGT
    GACCCAGCCT CCAAAATAGC ATCTTTGTTG CTAGCAAAGT TGGCTGATTA TGTTGTTCCA
    GGATGTCAGA CAGTTCTTTC TCCTACTGCT TCTGAACCAG ACAGCTCATT GTCCAAAAAC
    AGCCCAAAAA ATTCCCAGAA AGCAGATAAG GATGCAGAAG AAGAGAGTGA AGCAGTAGAT
    GGCAAGCTCT CCATTTTTAT TCATAAGCGA GAAGACCAGT CATCCCATGA GGTTCTTCAG
    CCTTTGCTTA GTAGTTCAGA GGGAAGGCCT TTTCGTCTTG GTACTGGAGC GAATATGGAA
    AAAGTAGTAA AGATGGATCG TGACATGACT AAGAGTGGAT GTTGTGAAGT AATCACAGAG
    GAGGCTGCAG CTGCTCTCCG CAAAGCCACT AAATGGGCCC AGTCGGGTTT AATTGTTAGC
    ATTGGGCCAC CTGTGGAGAC AGTTAGCCCT GATTCAGCGA GTGGACTTTC CACTGGTGAC
    AAAAAGAAAA CAGCCCAGAC CTCGATCTGT AGGGAAAGGA ATTCTGAACT TGCCAGGACT
    GATCCTGTAC GCCCTTTCAT TAGTGGCCAT GTAGCAAACA GCATGGCTGC AGAAGTAATT
    GCTCTGCTTC ACAGCCTGCT GATGGCACCA GAGTCAAATG CTGCCCAAAT CTGGACAACA
    ACAGCAGAGA AGGTTCTTTC TCGTGCCCTG ATGTATATCC CACAGCTTGG CAAATATGCA
    GAAAGCATCC TAGAGAACGG CAGCGGTAAT GGCAGGAAGC TAGCAAAGCT ACAAGGCATT
    GCAAGGCAAG CAGTTGCTGC ATTATGTGCG CTTGGTGGAT TTAAAGAAAC CATTAAAATA
    GGTTCTGAAG TCCAGGTGTT GGGTAAAGGA ATATCTGGGA GCATTGGAGT GGTTGTTTCT
    ATTAATGAAC AAGAAGGTAT TGCAACAGTG AAATTTCCAG ACATAGATTT TAGAAAATCA
    TGCAAAGCAT CTGACACACT GACAGTACCA TTATTTAGGC TCTGCGTTCC ACGGTCAGAG
    GCATTGCCTC TCCATAAACT GTCCATTACT GAGAAGGTAG TGCAGGCAGT CCAGTCCATG
    TTACTTCCCC AGGAGGGCTG CCTATCTATT CATACCTCGC TTCCTGCAAC AGGAGATGCC
    TCAACTCCAG TAATGGCAGT GGTTCGACTT CTTGCAGAGA TCAGGACAAG AGCTTGCTTG
    GTGATGGCTC AGCTGTTGGA GGACAGCTCA TTTTGTGAAG AATTCATACA GCAATGTCCA
    GCTGCTGTAG AAGTTCTAAA CCTAGTGGCC CAGGAATGCA GTCCTGGGGA GCGTCTCTCA
    GTGGTGGAAG TTCAGTGTGA AAGACTACGA ATGCTTTATC GGGACTGTGC TCGCCCCCCG
    CCACCACCTT TGCAGGCTGA GCGCAGACAG CCCAAGGAAA TCACCTGGTC TCCTTCCAGG
    GTATTCCCTC CTGTGAGAGC CTGTATGTTT TCATCCCATC TAACAGCAGT AACATTTCTG
    GCTGACCCAA GTGCTGGTGG GGGACTTCCT CGCGGCACAT TTATATATGC AACATCCCCT
    GTTCCAGTAC AGGCACCATC TTTTTACTGG GAAATAGAAG TAGTTTCCTA TGGAGACACA
    GATGATGACA CTGGCCCTAT TGTATCATTT GGGTTTGCTA CAGAAGCAGA GAAGCGGGAT
    GGAGCATGGA CAAACCCAGT CGGTACCTGT CTTTTTCATA ACAATGGTCG AGCTGTACAT
    TACAATGGAT CAAGTCTCTT ACAGTGGAAG AGTGTTCGAT TAGATGTGAC TCTTGCTCCT
    GGTGATGTTG CTGGAATAGG TTGGGAGAGA ACTGAAGGAA CACCACCACC CCCAGGGCAG
    CCTGCCAAGG GCAGAGTCTA CTTTACTTAC TGTGGACAGA GGCTTACTCC GTTCTTAGAG
    GATGTCTCTG GGGGCATGTG GCCAGTTGTG CATATTCAGA AAAAGAACAC TAAAGTCCGT
    GCAAATTTTG GATGCAGACA GTTTGCTTTT GCCGAGGGTC AAGCTCATCG CAATGCAGCA
    GATTTGTGCA TTGATTTGGC TGAAGAGATA AGTGCAAACT TTGAAGCATT GCCCTTTGCT
    ATGGCATCTG ACAGTGATAA TGATGCTGGG ACAAGCATAG CATCTGATCC TGGAACTCAT
    GGACCTCCTT GCCGTATTGC TGCAGTTTCT ACTGTGCAAC AGCAATATGA CAGTGATGCC
    TCATGCCGTT ACAAGGTAGA GCTGAGTTAT CAAGACTTTG TGACCTCTGG ACCTGATCCA
    CACCCACCTC CCATTGCAGA TGATGAAAGT GATGATGATG ACGATGATGA TATTCCTCGA
    GAGGATCATT ATGCTCTTTT GGTAAAAGCA TGGGAGACCA AAGTTTTTCC AACCATTCGA
    AGACGTTTCC GTAACGAGGC AGAGAGAAAG TCTGGCCTGG ACCAGATTAA GGGAGCTCTT
    CAGCTAGGAA TGGTGGATAT AGCGAGGCAA ACTGTAGAGT TCCTCTATGA AGAAAATGGT
    GGCATTCCAA GAGATCTCTA TCTTCCTACT ATTGAGGATA TCAAGGATGA GGCAAATAAA
    TTCACCATTG ATAAAGTTCG CAAAGGACTC ACAGTGGTCA TTCGATCCCC AGATAGCAAC
    AATGTAGCTG GGACAACTGT GGGAACAGCT TTACCCAAGT TTGCCATCCG TGGTATGCTG
    AAGACATTCT CTCTACATGG GGTGGTTCTG GATGTGGATT CAGTTAATGA GCTGGTTCAG
    GTAGAGACTT ACCTCCGGAG TGAAGGGGTA TTAGTGAGAT ACTGGTATCC CATAGATATG
    CTAGAAAGAC CTCCTGCGGG ATACAGGAGA ATGGCAACCA ATGGACTGGT GACTTTAGAT
    AGCACTAACA TCCAGATTCA TAGAGAGCTG TTGCGGTGTG AGTCTGCACT TGCCAGGCTT
    TACTGTCGTG TGGCTTTGCT TAACATCTTT GCTCCTAAGT CTCCGCACAC TTTCACTCGC
    CTGTTCCATA TTCCTGCTAT CCGAGACATT ACATTGGAGC ATTTACAGCT TCTGTCAAAT
    CAGCTTCTAA TGCCACCCCT TCCAGACGGA ACCATTACCT CCAGCTCTAT CCTTCTTGCC
    CAGTCCTTGC AACATTGCAT CTACTCCCAG AACTGCTCAA CAACAGATTT GTTCTACCAG
    GGCAATGCAC AGCCAATAAG AGAGTGGCTT GGTGTGGCCA TAACCAGGGC CCTGCATCAA
    GGAGAGGACA GTCTCTTGGA TCTGACAAAA CAAATCTGTT CCTTCTTGCA AAATGCGCCG
    GAACAGTTTC CATCTGAAGA ATTTCCCATC TCTGAATCCA AGCTCAGTAT GGATGTTAAT
    TTTCCTGGGG CAGCTTTTGT TATTGTTTCT TGTATAGAGA GTCCGTCTGG ATTTAGAAAA
    GACTCCTCCT TGTACAAGGC TCCCTGGTCT AGAGTCATGG TATATGGCCT TGGACATAAA
    GTAAAGAGGA ATGGACAGCT GAACCTGATA GAATCAGTTT GTTATCCACG TGATGCCTCC
    CCAAGTAACA CAGGCCTCAC TCCCCCACCA ACCACAAACC AGTACCCAAC AGCGATTATT
    CCCACTGACA AGGTCCACGT CAAACTTGGT GTATCACCTA CTCCTGGTGC TGTGCTGGTC
    ATCCATTCCC TGCCTCTAGA GTTTCCGTTG GCAATGGCAT TCACAGAACA GCTGTTAACC
    TGGAAGTTGG AGGACACAGA AGAGAAGTCA GAAGATGAGC TTGATACTGT TCCATCCTCT
    GCTCTGTTGC AAGTGGTAGA ACTTTTAGGT ACTTTGATTT GGACAACAGA CCTTGCTGCA
    GTAATTAAGG AGCTTCTGTT CCATCTGTTG GCTGAACTAT TAAGGAAAGT TCACCATCTA
    GAGCAGAAGA AGAACCCAAG TGGATTATCT TCCTCGATAG CCCTGCAGTT GAATCCATGT
    CTAGCAATGC TTATGGCTTT ACAGACAGAA CTGCGCAAGC TGTATGATGA AGAAACTCAA
    AACTGGGCAT CTGGGAATGC ATGTGGGGGG TCTGTACCTG GAGCCTCTGA GCAGGGAAGA
    TTTTCAACTT ACTTTCATGC CCTCATGGAA GTGTGCCTGG CAGTGGCAGA AGTGACGCTC
    CCAATCAACA TGAGCTCAGC AATCAGTGTG ATGGCATCTA CAAATGCCAC TAATCTGAGT
    GACTCTTCTT CTTCCTCTTC CTCTTCCCCT GGACAGACTC CTCAAAGTCC AAGTCTGTTA
    TCAAAGAGAA AGAAAGTTAA GATCAAACGT GAGAAGACTG CATCTGGAAA ACGTACACCT
    TCACGTGGTG CAGAGAGTGA TGCTGCTTTG CTTAGCATTG GAGGAAGCAA ACCTGAGGAC
    ATGTTGTGGT TCCATAGGGC ATTGACTTTA TTGATGATCC TAAGGCATCT AACTAACAAG
    GATCCACAGG GGATGGGTGT CACAAACGAT GCTATTGCTG ATGCTAGCCA GGCTTTAGTT
    GGTCCCACAG CTCACAGTCG TCTCCTTGTG ATTTCTGGCA TCCCTACACA CCTAGAAGAA
    TCAGTGGTCA GAAGTGCAAT TAGAAAGGCA TGTAATGCTC ATGGTGGAGT CTTTAAAGAT
    GAAATCTACA TTCCATTGCA GGAAACTGAA ACCCCTAAAC CCAAAGACAG AATAGAAGGG
    GCAGAATGCA AGACAGAAAT AGAAAAACCC TCTACGCAGC ATACAACCGA CAATATGGAT
    ATCAGCAGCT CGAGCAGTGT AACTCCTGCA GCAAGTGTCA CGGCATCAGC TTCTACAAGC
    CAAGCTTCAT TGTGCAGCTC CCAAGGGGGG GTATCACGGA CCACAAGTGA AACATCTATG
    GAGCAGTTTG CCCCAGAACA TGCTGTGCCA TCAGGGCTTC TGGAACCAAC TATTATCTCC
    AGCCAGGAGA GTCTGGATCT TTCTATGTGC AGCACAGGAA GCCTCGGCAG TCTGGGAAGT
    CTTGTTGAAC AGCTTGATAA TGTTGAAAAC TCTTTGTCTG ACGTGGGCTC CCTATACACA
    GCTACATCAC TAGATAGCCA TATGGCAGAG TCGCGGCCAA TCAAAGGATT TGCAGTTGTA
    GAGGTGCGAT CACGAGCTAA AATAGAGAAA ATCCGTGCCA CTTTATTTAA CAGTAGTGAC
    CTCATTGGGT TATCTGGTTT GGATGGTGAA GATGAGCTGA TGGAAATGTC TACTGAGGAG
    ATCTTGACTG TTTCAACAGT GAATCAGAGC TTGTTTGATA CCCAAGGAAT ACCCGGTCTG
    GCTGATTATT TCATGGATAA ATCAATGAAA GGTGATAAAT TAGTCCCGGA AGCTCGTGAT
    GTTCTGACTG AGATTTTCAA GAGCTGCATT CACGCCGAGC AGATGCTGAG TCTGACTCCA
    GCCAAGTTTA TCAAAGTGTC TGATATCTAC CTTAGCAAGG AGCAAATCAA TTCACAGACC
    CCAGGGAACT TACTGCATAC CTTTTTCACC AATGTTCGAC CACCTAAAAA AGTCCTGGAA
    GATCAACTAA CGCAGATCTT AAGAAAATAT GGAATTCCCA AGCCTAAATT TGACAAAAGC
    AAATACAGCA AAACTGGGAA GGAGCAACAT CCTGTGAAAG TTGTAAGCAC CAAAAGACCT
    GTGGGCAAAC CACCGACCAA AGACAAGGCT GTATTCAACA GCGTCAGGAC TGCTTTAAGT
    GAGAAAAAAC CAACTGTGAA GCCAAAATCT CCGGAGAAGG GCAGGCCAGA TGAGAAGGAC
    CCAGACAAAT CTCCAACAAA GAAGCAAGAA AGGTTTCACT TGTGTCCCTA TCTAGTTCCC
    GAGGAGAGAT ATCTGACGTT GGATGGATTT CACCGCTTTG TAGTAGATCG GGCTAAGCAA
    GACATTCGAA GCATCTGGAG AGCTGTCTTG GCGTGTGGAT ATGATCTTCA TTTTGAAAGG
    TGTGCCTGCA TTGATACAAG GCATGCTCAG AAATCTAGCA GAAAATGGAC CTTGGAAATG
    GATGTTGCTC TTGTTCAGTA TATCAACCGC CTTTGCCGTC ACCTTGCCAT AACGCCAGCC
    CGACTACACC CTCATGAGGT TTATCTGGAT CTTGCAGATA CGGCTGACCC AAGAGTCTCC
    TGCCTGCTGA ATGTACCAAT AGAAAGCCTG CGCCTCCGAT TTGCTCTTCT TCAGTCCCTC
    AACACTACTT TAGAGACCTT TTTCCTGCCC TTAGTGGAGC TACGGCAAAC AGACGTATAC
    ACACACAGCA TCGCATCCCT ACTCCAGCTA GCTAAAGGGT TAATCTTTTA CGATACAAAA
    GTGAATGTGA TGAACCGGGT ATTGAACGCA ACAGTGCAGA GGACAGCAGA TCATGCAGCC
    CCTGAGATCA CGCTGGACCC ACTAGAAATT GTAGGAGGGG AAATTCGTAC CTCAGAAAAC
    TCCTACTTCT GCCAGGCAGC CCGACAGTTG GCATGCATCC CTTCCTCACA ACTGTGTGTC
    AAACTGGCAA GTGGAGGAGA CCCCACGTAT GCATTTAACA TCCGCTTTAC TGGAGAGGAG
    GTGCATGGTA CAAGTGGCTC ATTCCGTCAT TTCCTCTGGC AAGTGTGTAA AGAACTACAG
    AGCTCCTCGC TGTCTCTTCT ACTGCTCTGC CCCAGCTCTG CAGTTAATAA GAATAAGGGA
    AAATATCTTC TAACTCCCAG TCCCATAACA TATGCAGAAG AGCAGTTGCT CCATTTCTTT
    GGCCAACTAC TGGGAATAGC CATCAGAGCC GATGTTCCAC TCCCTCTGGA TCTACTCCCC
    TGCTTCTGGA AGATGCTTGT AGGAGAACCT TTGGACCCAG AAACTGATCT TTATGAAGCA
    GATATTCTCA CCCACAACTA CATCAAGAAG TTTGAAAATG TAAATGATGA GTCAGAGCTG
    GAAGCTCTTT GTGCTGAAAT TGCCTCTCAG CATCTTCCTA CCGAAAGTCC AGATTCTCCT
    CATAAACCAG CCTGCAAATT TACATACTTG ACAATGACTG GTGAAGAAGT GGAGCTGTGC
    CCTAAGGGAA GACACATTTC TGTAGTCTGG GAAAACAAAG ATATATATGC AATGGGAATC
    CGGAACCTTC GTATACGTGA GCTGAAGAAC CAGGAGTGTA TGAGCGCAGT AAGGGCTGGG
    CTGGGCTCCA TTATACCTCT GCAGCTCCTC ACCACTCTAA ATGCTCTAGA GATGGAACTA
    AGGACATGTG GCCTACCATA TATTAATTTG GAATTCTTAA AGGCTCACAC CATGTATCAA
    GTGGGTCTAA TGGAGACAGA TCAACACATT GAGTATTTTT GGTCTGCCTT AGAAATGTTC
    ACTCAGGAAG AACTTTGCAA ATTCATAAAA TTCGCCTGCA ATCAGGAACG CATTCCCTTT
    ACATGTCCAT GCAAAGATGG TGGGCCAGAC ACGGCTCATG TTCCACCTTA TCCTATGAAA
    ATAGCACCAC CCGATGGAGC AACAGGCTCC CCAGATTCCA GGTACATCCG AGTGGAGACA
    TGTATGTTCA TGATCAAGCT TCCGCAGTAC TCGTCTCTAG AGATGATGCT GGAGAAACTG
    AGATATGCCA TTCACTACAG GGAAGACCCA CTCAGCGGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012824247.1
    CDS321..13562
    Translation

    Target ORF information:

    RefSeq Version XM_012968793.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 4 (hectd4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012968793.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGGGGTCGG CGGCTGCCGG GGACTCGGCT CAGTGGCTGT CGGTGAAAGA AGAGACTATC 
    TTTTTACATG ATGGGCTGAT CCGGGTCACA GACCTGGCGG AGTTGCCCAG CGAAATCGGG
    GTCAGTGAGG CCGGGGACGC AGAACTGGAG ATATTGACTT TTGAGACTAA GAACCCTATT
    GAGCTAGCAG AGCATTTACG TGCAGTGTGT GGGAACCAGA GCAATGCCTA TGCACGATTG
    CTGGAATACC GCTTGAATGC GTTACGTGGG TTATGGAATG CCCAACGCCA GCTGGCGCTT
    GAGGATCAAC ATGAAAGAGA TGGGGCTGAT GACGAAACCT TGGCCCTGCT TAAACGGCAG
    GGTTTGTTGC AACAGCCGGA ACAGGCTCCA TTTACATCTC GAATGGGGCT TTTACTGGTG
    TTCCCCCTTA TCCAGTCCCA AAGTAAAACA GACCCTTCAC TTTGTAACTT GACAGCTGAG
    GTGCTTTTAA ATTGCCTTCG GGACTGCCAA CCCCTGAGCT TGACTAAAGA ACCAGCTGAC
    TGCCTGAATG GAATAGAGTC CTTACTGTGT TCTTGGTTAG AAGACACTCC AAATGTGGGC
    CAACAAGTCT CCTTTAAGCA AAAGGAAAAT TCTGCTGCTG CTTTGGTTGC CTTAGCTTGT
    GCTAGGGGAT CATTAAAAAC ATTTATTCAC ACTGTACACT TGCTGCAAAA ACAGACTGAC
    CTAGGATCCC TTCCTGTGGC AGATGTATTG TATAGGTTGC TGCTTTTAGA GGGAGGTCCA
    GGTTCTCCCT CTTGTTTACT GGGAAGCAAA CACATTGTAT CCTGGGGTTT TGAGGATATG
    CTTCCAGCTC CTGATGGGAT CAGTGGTTCA AACAATGAGA ACAAAGATGC AGATTTGGGT
    CGCTGTGTAG CATGCGATGG GCTTTATCTT TACACAACTA ACTCTGTAGG TAGAGGATTG
    AGCAAAGTAG GGTCTGGGCT GCATGGCACT TTACGAGGTT TTGTTTATTC CCGAAACGAG
    GAGCTAGAGG CTGGCTGGAT TGCTTATGGA AGTGGGAAGA TACTTCACAG GCCTGCCTCC
    TTTGATAACA AACCTCAATA TCTTTTCCAA TTAATTGACC AATACACACT TCAGGTCTCT
    TATATAGTCA GCATGCCAGC CAATCATTTC CCGGTTGGCA GCAATATGAC TACGGTGCAC
    CTCTCCTCGG ATGGCACGTA TTTTTACTGG ATTTGGTCTC CAGCTAGTTT AAATGAGAAA
    ACCCCCAAAG GACACTCTGT CTTCATGGAT ATATTTGAGT TAAATGTGCA AAATGGAGTA
    TGCATTGCAA GCCCTCTTCA TGAGCGCATT ATATTAATGA GGAAAGAGGG AGAATCTGCT
    AAAAGTATTA ATGAAATGCT TTTGAGCAGA TTATCACGAT ACAGAGCTTC TCCTTCAGCA
    ACTCTAGCAG CACTTACAGG ATCTACAATC TCTAACACTC TAAAAGAGGA CCAGACTGCT
    GCAAATACGA GCTGCGGGCT TCCTTTGAAA ATGTTAAGAA AGACTCCAAT GTATACTTGT
    GGCACATACT TAGTACTACT CGTGCCACCT CCGGGTGGGT CAGGAAGTTC AGCAACAAGA
    TCTTTGTTTG GTGGAACCAG CGGCCTCTCC TCTTTAAAAA TTCTGGCTTC CAGCTTGGTA
    TTTAACATTT CTGATGGGCA GTTCACCAAC AGAGCAGATC TTATTGATGC ATCTGGAAGT
    TCTCTTGGAA GGGGTGCTTT GGTGACAGGC CTAGGTGCCT GCTATGATAC AATGAATAAC
    ATGATCTGGA CGTGCTCCAA TGATTACATC GATCAGTGGT GTAATCCTGG AAACCAGGCT
    TTCCATGTGG TGTGTCAGAG GCTTGGTGTC ACTCACATCA TTGCTGATCC CAAAGGAGAG
    GCAGTATCAA CAAATGAAGT TATAAACCAG TTGCTTCACC ATGTGGGTGC TATGTGTATT
    CACCAACTCA ATCTCCTGGC TAATAGCAAC AGTTCTCCAA TCAGTACCTT TCTGGGCAAA
    CAACATCCTA TAGAGCCACA TCATCTTAAT AGTATATGTG ACATCATGGA GAAAGCCATG
    GTCAATGGTG ATAAATGCAT TATTCGCTGC ATCTTGGTTG TTTTCCAGGT GGTATTTAAA
    TTCTTTCTAA ACCCACAAAC TGAGAGGAAT CGAGAAACCA TAAGGAGGGC TGGTTTGCTC
    CTTTGGCAGT TGCTCATGGC GCCTAAGGAC CAAATTTGTC CTGAAATACA GAGAGAGGTC
    TGCTTGGCTA TTAGCTTTGG TTTAAACATT TTGAATCCAG GAGAGCTAGA AATTAATAAC
    TTGCTCAAGC TGATTTTAAC TGAAGGGGAC AGAAATTGTG GCCTCTCCCA ACTCCGTGAT
    GTCATTTTGA CAAACCTCAC TGAACAGCTT CAGAATAACA GGTTTGGAAG TGAAGATGAT
    GAACATTACA GGCTTAATGA TGAACTATTG CACTATATTT TGAAGATTGT TGTTCGTGAA
    TCCTGTGTTC TGATCACCAA GTGCCAAACA GTGTCTAAAG AAGAATTCCA AAGACTCTTG
    TCAACAGTGC CTGTGGCTTC TCCCTGTCTA TGCTATTTAA TGGCTGTACA GAATCATCTC
    CTGAGTAACA CTGTCTTAAT TAAACCTGAT GACAGCGATG ACAGTGACAA CTCCCTGCAG
    GGGGAGACAT TGAAGGTACA GGAACTCCAG GCCAGCATCT TGGCTTTGGC AACCCAGATA
    CTTTCTGGTT GTGATGAAGT TCTGGATATT CTGCAGCGAG TGACCACTGC TCTTATCAAC
    AGCGACCTGT TAGACAGAGA TTTAAGACTG AAAGGCCTTG AACAGATCAC AAAGTCCACC
    CTGCTTGGCC ATCTCCTCCC AGTACTTCTC ACATCCCTTA TGCATCCAAA CTTACAGACA
    TTAACCATGG CAGATGCGCT TATGCCTCAG CTGGTGCAGC TTGTTCTTTA CACTAGCCAG
    ACTGCACTGC TGCTTAAAAC CCAGTGTCCA GTATTGGCAG ATACAGGGGA CACCACCAGT
    GGAATTCCAG AGCAAAAAAA CAAACTGTTA ACTGATGACA GGATGCTGGA AGAAAAGGAG
    GAGCCTGGTT TCCTCACTGG GCTAAAGATT CCTGCGCCAT GGGCTGCAGG AAAGACTGTG
    GAAACATCTC ATCCAGTCAG AGACAATTAC AAGTTTAAAG AGACTGTGCA CATTCCTGGA
    GCTCGTTGTT TGTACCTCCG GTTTGACAGC AGGTGCTCTT CACAGTACGA TTATGATAAG
    CTGGTGATAT ATGCGGGACC CAACACAAAC AGCAGAAAAG TTGCCGAATA TGGTGGAAAT
    ACCCTGGGGT ATGGCAGTAG AAGTGTTTTA GGTACAGGCT GGCCAAAAGA TTTGGTTAAG
    GTGGAAGGTG ATACTGTGAC ATTCTCCTTC GAAATGAGAA GTGGGCGTGA GCACAACACT
    CCTGATAAAG CAATGTGGGG TTTTGCGTGC ACTGTCAGAG CTCAGGAGTC CTCAGAAGAT
    GTGTCAGGGG GCTTGCCATT TCTAGTAGAC CTGGCTTTAG GTCTGTCTGT CTTGGCTTGT
    TCCATGCTGC GTATATTGTA CAATGGTCCA GAAATCACAA AAGAAGAGTT GACTTGTCAC
    GATCTGTTGA GGTCAAAGCT TTTGCAGAGG TGTCAGTGGC AGGTGGAGGC TAATGGTGCT
    TTATCCCCGG CACTCACCCC AAGCCCTTCG CCGCTGCCCC TTACTATAGA AGAAGACCAA
    GAATTTACGT ATCCATCTGA CATCTTGTTG CCACCAGTTG GGAACTATTT TGAACTGCCA
    AGAATCAGAC TGCCTCCTGG TATTATGATC AAGCTCAGGG AAATATCTGG ACGCGCTCGA
    CCACAGTTTA GACCAAGCAT AAAAGAGGTT ATCCAGCCTG ATGTGATGGA AGAAATGGTT
    GTATCTTGCG TCATTAAACA CTTGAACCTG GTCGATGCAT TGCAGTCTTT AATAAACTTC
    CAGTATCGAG AAGAACATCT AGAAGAATAT GATTTATTAT GTAAAATAAT GGGGGAAACT
    TTTAAGAAGC TCAATGCAAT GGAAAGACAA TTGCAGAGTG TTGCAGAACT GGAACAAAAG
    TGGCAAAGTG AAGTGGAAGA TGCTCAGAAT GGAAAGCTGG AGAACAACGC TCCCTTTTTT
    TACGACTATC ATTTTAATGA GAGCAAGTTA AAGGAACTTG AACTTTTATG TTCTATGAAA
    GAGGTCCCTT CTGATGGAAG TGATCCAGAA AACATGGTTT TCTCTCTGCG AGAGAAGTTT
    TTTCAAGAGG TGAACTCCCT GATCCAAAAA CCTTCCCACC CAATGGCTAA GACCAAAACA
    TTGGTTAAGA GTCTGATGAA TAGAGCAGAG CTCCTGTTAC ATGTGACTAT TGCAGCACAT
    TCTGGTATCA CGAGAAGCAT ATCTGGCACT CCAACTGAAC CTCAAGCTGG CAAGTCTATC
    TCAGAAGCAA AAGTTATATC TCAAGCCATG CGCCAGCCTG TTTTCCTCCG CAGCATGTCT
    GCCCCTTCTG ACCTGGAAAT GATTGGAAGT AGTGACTTAG AGTTCAGCAG GTCTAATCCA
    AGGAGAAGGA ACATCACTAG CCACAGGAGC AGCTCCTTCA CTCTGCTTCA GTCTTTAGCT
    ATTGAAGACT GCAAGGATAA ACCAACATAC AGTGTCCTTT TAGGGCAACT GTTTGCCTTC
    ATTGGGACCA ATCCCGACCA AGCAGTATCC AGTAGCAGCT TTCTACTTGC AGCCCAAACC
    AGATGGCGTC GTGGTAATAC TCGCAAACAA GCCCTTGTAC ACATGAGAGA ATTACTGACT
    GCTGCTGTCC GTGTTGGGGG TGTGACACAT TTGGTTGGAC CAGTAACAAT GGTGCTACAG
    GGAGGACCAA GAATTGAAGA GCTCACCTGT GGAGGAATGG TGGAGCTTGT TCAAGAGGCA
    TTTGGAGAGA CCATGACCTC AGTGGTGTCA CTTTGTGCAC GGTATCCCAT TGCCTGTGCA
    AATAGCATTG GGCTTTTGTG TACTATACCT TACACAAGGA GTGAAGAAAA ATGTTTAGTT
    CGCAGTGGGC TTGTTCAACT GATGGACCGA CTCTGCAGCT TAAGCAACCA GAGAGAGTCC
    AGCTCAAATG AAAAGCAAAC CAAGAAACAG AAAGTAGCAA CTATGGCATG GGCAGCCTTC
    CAAGTACTGG CCAATAGATG TGTAGAATGG GAGAAAGAAG AAGGTGGTTC TACTGAGGCG
    GTACACTCTG GTTTGGCTAG ACAAGTCTCC ACCCTCCTCA CTAACCATCT AGCAAGGGCT
    ACAGAGTGCT GTGGAAACCA AGCTGCAGGG AATGATGCCC TACAGGACGT TCTGAGCCTT
    CTCAATGATC TCTCTAGGAG TCAGATAGGG AAGGCAATTC TGAGCCAGCC TGCTTGTGTG
    TCCAAACTTC TTTCGTTGCT GCTGGACCAG CGTCCTTCTC CAAAGCTTGT GCTCATAATT
    CTGCAGCTGT GCCGCGCTGC ACTTCCTCTC ATGAGTGTTG AAGACTGTGG AAATGTAGAA
    CTTCCTCCAT GGAGTTACTC TTTACCAGCT CTGGACAGTG AACAGGGGGA GGATCCAAGT
    GACCCAGCCT CCAAAATAGC ATCTTTGTTG CTAGCAAAGT TGGCTGATTA TGTTGTTCCA
    GGATGTCAGA CAGTTCTTTC TCCTACTGCT TCTGAACCAG ACAGCTCATT GTCCAAAAAC
    AGCCCAAAAA ATTCCCAGAA AGCAGATAAG GATGCAGAAG AAGAGAGTGA AGCAGTAGAT
    GGCAAGCTCT CCATTTTTAT TCATAAGCGA GAAGACCAGT CATCCCATGA GGTTCTTCAG
    CCTTTGCTTA GTAGTTCAGA GGGAAGGCCT TTTCGTCTTG GTACTGGAGC GAATATGGAA
    AAAGTAGTAA AGATGGATCG TGACATGACT AAGAGTGGAT GTTGTGAAGT AATCACAGAG
    GAGGCTGCAG CTGCTCTCCG CAAAGCCACT AAATGGGCCC AGTCGGGTTT AATTGTTAGC
    ATTGGGCCAC CTGTGGAGAC AGTTAGCCCT GATTCAGCGA GTGGACTTTC CACTGGTGAC
    AAAAAGAAAA CAGCCCAGAC CTCGATCTGT AGGGAAAGGA ATTCTGAACT TGCCAGGACT
    GATCCTGTAC GCCCTTTCAT TAGTGGCCAT GTAGCAAACA GCATGGCTGC AGAAGTAATT
    GCTCTGCTTC ACAGCCTGCT GATGGCACCA GAGTCAAATG CTGCCCAAAT CTGGACAACA
    ACAGCAGAGA AGGTTCTTTC TCGTGCCCTG ATGTATATCC CACAGCTTGG CAAATATGCA
    GAAAGCATCC TAGAGAACGG CAGCGGTAAT GGCAGGAAGC TAGCAAAGCT ACAAGGCATT
    GCAAGGCAAG CAGTTGCTGC ATTATGTGCG CTTGGTGGAT TTAAAGAAAC CATTAAAATA
    GGTTCTGAAG TCCAGGTGTT GGGTAAAGGA ATATCTGGGA GCATTGGAGT GGTTGTTTCT
    ATTAATGAAC AAGAAGGTAT TGCAACAGTG AAATTTCCAG ACATAGATTT TAGAAAATCA
    TGCAAAGCAT CTGACACACT GACAGTACCA TTATTTAGGC TCTGCGTTCC ACGGTCAGAG
    GCATTGCCTC TCCATAAACT GTCCATTACT GAGAAGGTAG TGCAGGCAGT CCAGTCCATG
    TTACTTCCCC AGGAGGGCTG CCTATCTATT CATACCTCGC TTCCTGCAAC AGGAGATGCC
    TCAACTCCAG TAATGGCAGT GGTTCGACTT CTTGCAGAGA TCAGGACAAG AGCTTGCTTG
    GTGATGGCTC AGCTGTTGGA GGACAGCTCA TTTTGTGAAG AATTCATACA GCAATGTCCA
    GCTGCTGTAG AAGTTCTAAA CCTAGTGGCC CAGGAATGCA GTCCTGGGGA GCGTCTCTCA
    GTGGTGGAAG TTCAGTGTGA AAGACTACGA ATGCTTTATC GGGACTGTGC TCGCCCCCCG
    CCACCACCTT TGCAGGCTGA GCGCAGACAG CCCAAGGAAA TCACCTGGTC TCCTTCCAGG
    GTATTCCCTC CTGTGAGAGC CTGTATGTTT TCATCCCATC TAACAGCAGT AACATTTCTG
    GCTGACCCAA GTGCTGGTGG GGGACTTCCT CGCGGCACAT TTATATATGC AACATCCCCT
    GTTCCAGTAC AGGCACCATC TTTTTACTGG GAAATAGAAG TAGTTTCCTA TGGAGACACA
    GATGATGACA CTGGCCCTAT TGTATCATTT GGGTTTGCTA CAGAAGCAGA GAAGCGGGAT
    GGAGCATGGA CAAACCCAGT CGGTACCTGT CTTTTTCATA ACAATGGTCG AGCTGTACAT
    TACAATGGAT CAAGTCTCTT ACAGTGGAAG AGTGTTCGAT TAGATGTGAC TCTTGCTCCT
    GGTGATGTTG CTGGAATAGG TTGGGAGAGA ACTGAAGGAA CACCACCACC CCCAGGGCAG
    CCTGCCAAGG GCAGAGTCTA CTTTACTTAC TGTGGACAGA GGCTTACTCC GTTCTTAGAG
    GATGTCTCTG GGGGCATGTG GCCAGTTGTG CATATTCAGA AAAAGAACAC TAAAGTCCGT
    GCAAATTTTG GATGCAGACA GTTTGCTTTT GCCGAGGGTC AAGCTCATCG CAATGCAGCA
    GATTTGTGCA TTGATTTGGC TGAAGAGATA AGTGCAAACT TTGAAGCATT GCCCTTTGCT
    ATGGCATCTG ACAGTGATAA TGATGCTGGG ACAAGCATAG CATCTGATCC TGGAACTCAT
    GGACCTCCTT GCCGTATTGC TGCAGTTTCT ACTGTGCAAC AGCAATATGA CAGTGATGCC
    TCATGCCGTT ACAAGGTAGA GCTGAGTTAT CAAGACTTTG TGACCTCTGG ACCTGATCCA
    CACCCACCTC CCATTGCAGA TGATGAAAGT GATGATGATG ACGATGATGA TATTCCTCGA
    GAGGATCATT ATGCTCTTTT GGTAAAAGCA TGGGAGACCA AAGTTTTTCC AACCATTCGA
    AGACGTTTCC GTAACGAGGC AGAGAGAAAG TCTGGCCTGG ACCAGATTAA GGGAGCTCTT
    CAGCTAGGAA TGGTGGATAT AGCGAGGCAA ACTGTAGAGT TCCTCTATGA AGAAAATGGT
    GGCATTCCAA GAGATCTCTA TCTTCCTACT ATTGAGGATA TCAAGGATGA GGCAAATAAA
    TTCACCATTG ATAAAGTTCG CAAAGGACTC ACAGTGGTCA TTCGATCCCC AGATAGCAAC
    AATGTAGCTG GGACAACTGT GGGAACAGCT TTACCCAAGT TTGCCATCCG TGGTATGCTG
    AAGACATTCT CTCTACATGG GGTGGTTCTG GATGTGGATT CAGTTAATGA GCTGGTTCAG
    GTAGAGACTT ACCTCCGGAG TGAAGGGGTA TTAGTGAGAT ACTGGTATCC CATAGATATG
    CTAGAAAGAC CTCCTGCGGG ATACAGGAGA ATGGCAACCA ATGGACTGGT GACTTTAGAT
    AGCACTAACA TCCAGATTCA TAGAGAGCTG TTGCGGTGTG AGTCTGCACT TGCCAGGCTT
    TACTGTCGTG TGGCTTTGCT TAACATCTTT GCTCCTAAGT CTCCGCACAC TTTCACTCGC
    CTGTTCCATA TTCCTGCTAT CCGAGACATT ACATTGGAGC ATTTACAGCT TCTGTCAAAT
    CAGCTTCTAA TGCCACCCCT TCCAGACGGA ACCATTACCT CCAGCTCTAT CCTTCTTGCC
    CAGTCCTTGC AACATTGCAT CTACTCCCAG AACTGCTCAA CAACAGATTT GTTCTACCAG
    GGCAATGCAC AGCCAATAAG AGAGTGGCTT GGTGTGGCCA TAACCAGGGC CCTGCATCAA
    GGAGAGGACA GTCTCTTGGA TCTGACAAAA CAAATCTGTT CCTTCTTGCA AAATGCGCCG
    GAACAGTTTC CATCTGAAGA ATTTCCCATC TCTGAATCCA AGCTCAGTAT GGATGTTAAT
    TTTCCTGGGG CAGCTTTTGT TATTGTTTCT TGTATAGAGA GTCCGTCTGG ATTTAGAAAA
    GACTCCTCCT TGTACAAGGC TCCCTGGTCT AGAGTCATGG TATATGGCCT TGGACATAAA
    GTAAAGAGGA ATGGACAGCT GAACCTGATA GAATCAGTTT GTTATCCACG TGATGCCTCC
    CCAAGTAACA CAGGCCTCAC TCCCCCACCA ACCACAAACC AGTACCCAAC AGCGATTATT
    CCCACTGACA AGGTCCACGT CAAACTTGGT GTATCACCTA CTCCTGGTGC TGTGCTGGTC
    ATCCATTCCC TGCCTCTAGA GTTTCCGTTG GCAATGGCAT TCACAGAACA GCTGTTAACC
    TGGAAGTTGG AGGACACAGA AGAGAAGTCA GAAGATGAGC TTGATACTGT TCCATCCTCT
    GCTCTGTTGC AAGTGGTAGA ACTTTTAGGT ACTTTGATTT GGACAACAGA CCTTGCTGCA
    GTAATTAAGG AGCTTCTGTT CCATCTGTTG GCTGAACTAT TAAGGAAAGT TCACCATCTA
    GAGCAGAAGA AGAACCCAAG TGGATTATCT TCCTCGATAG CCCTGCAGTT GAATCCATGT
    CTAGCAATGC TTATGGCTTT ACAGACAGAA CTGCGCAAGC TGTATGATGA AGAAACTCAA
    AACTGGGCAT CTGGGAATGC ATGTGGGGGG TCTGTACCTG GAGCCTCTGA GCAGGGAAGA
    TTTTCAACTT ACTTTCATGC CCTCATGGAA GTGTGCCTGG CAGTGGCAGA AGTGACGCTC
    CCAATCAACA TGAGCTCAGC AATCAGTGTG ATGGCATCTA CAAATGCCAC TAATCTGAGT
    GACTCTTCTT CTTCCTCTTC CTCTTCCCCT GGACAGACTC CTCAAAGTCC AAGTCTGTTA
    TCAAAGAGAA AGAAAGTTAA GATCAAACGT GAGAAGACTG CATCTGGAAA ACGTACACCT
    TCACGTGGTG CAGAGAGTGA TGCTGCTTTG CTTAGCATTG GAGGAAGCAA ACCTGAGGAC
    ATGTTGTGGT TCCATAGGGC ATTGACTTTA TTGATGATCC TAAGGCATCT AACTAACAAG
    GATCCACAGG GGATGGGTGT CACAAACGAT GCTATTGCTG ATGCTAGCCA GGCTTTAGTT
    GGTCCCACAG CTCACAGTCG TCTCCTTGTG ATTTCTGGCA TCCCTACACA CCTAGAAGAA
    TCAGTGGTCA GAAGTGCAAT TAGAAAGGCA TGTAATGCTC ATGGTGGAGT CTTTAAAGAT
    GAAATCTACA TTCCATTGCA GGAAACTGAA ACCCCTAAAC CCAAAGACAG AATAGAAGGG
    GCAGAATGCA AGACAGAAAT AGAAAAACCC TCTACGCAGC ATACAACCGA CAATATGGAT
    ATCAGCAGCT CGAGCAGTGT AACTCCTGCA GCAAGTGTCA CGGCATCAGC TTCTACAAGC
    CAAGCTTCAT TGTGCAGCTC CCAAGGGGGG GTATCACGGA CCACAAGTGA AACATCTATG
    GAGCAGTTTG CCCCAGAACA TGCTGTGCCA TCAGGGCTTC TGGAACCAAC TATTATCTCC
    AGCCAGGAGA GTCTGGATCT TTCTATGTGC AGCACAGGAA GCCTCGGCAG TCTGGGAAGT
    CTTGTTGAAC AGCTTGATAA TGTTGAAAAC TCTTTGTCTG ACGTGGGCTC CCTATACACA
    GCTACATCAC TAGATAGCCA TATGGCAGAG TCGCGGCCAA TCAAAGGATT TGCAGTTGTA
    GAGGTGCGAT CACGAGCTAA AATAGAGAAA ATCCGTGCCA CTTTATTTAA CAGTAGTGAC
    CTCATTGGGT TATCTGGTTT GGATGGTGAA GATGAGCTGA TGGAAATGTC TACTGAGGAG
    ATCTTGACTG TTTCAACAGT GAATCAGAGC TTGTTTGATA CCCAAGGAAT ACCCGGTCTG
    GCTGATTATT TCATGGATAA ATCAATGAAA GGTGATAAAT TAGTCCCGGA AGCTCGTGAT
    GTTCTGACTG AGATTTTCAA GAGCTGCATT CACGCCGAGC AGATGCTGAG TCTGACTCCA
    GCCAAGTTTA TCAAAGTGTC TGATATCTAC CTTAGCAAGG AGCAAATCAA TTCACAGACC
    CCAGGGAACT TACTGCATAC CTTTTTCACC AATGTTCGAC CACCTAAAAA AGTCCTGGAA
    GATCAACTAA CGCAGATCTT AAGAAAATAT GGAATTCCCA AGCCTAAATT TGACAAAAGC
    AAATACAGCA AAACTGGGAA GGAGCAACAT CCTGTGAAAG TTGTAAGCAC CAAAAGACCT
    GTGGGCAAAC CACCGACCAA AGACAAGGCT GTATTCAACA GCGTCAGGAC TGCTTTAAGT
    GAGAAAAAAC CAACTGTGAA GCCAAAATCT CCGGAGAAGG GCAGGCCAGA TGAGAAGGAC
    CCAGACAAAT CTCCAACAAA GAAGCAAGAA AGGTTTCACT TGTGTCCCTA TCTAGTTCCC
    GAGGAGAGAT ATCTGACGTT GGATGGATTT CACCGCTTTG TAGTAGATCG GGCTAAGCAA
    GACATTCGAA GCATCTGGAG AGCTGTCTTG GCGTGTGGAT ATGATCTTCA TTTTGAAAGG
    TGTGCCTGCA TTGATACAAG GCATGCTCAG AAATCTAGCA GAAAATGGAC CTTGGAAATG
    GATGTTGCTC TTGTTCAGTA TATCAACCGC CTTTGCCGTC ACCTTGCCAT AACGCCAGCC
    CGACTACACC CTCATGAGGT TTATCTGGAT CTTGCAGATA CGGCTGACCC AAGAGTCTCC
    TGCCTGCTGA ATGTACCAAT AGAAAGCCTG CGCCTCCGAT TTGCTCTTCT TCAGTCCCTC
    AACACTACTT TAGAGACCTT TTTCCTGCCC TTAGTGGAGC TACGGCAAAC AGACGTATAC
    ACACACAGCA TCGCATCCCT ACTCCAGCTA GCTAAAGGGT TAATCTTTTA CGATACAAAA
    GTGAATGTGA TGAACCGGGT ATTGAACGCA ACAGTGCAGA GGACAGCAGA TCATGCAGCC
    CCTGAGATCA CGCTGGACCC ACTAGAAATT GTAGGAGGGG AAATTCGTAC CTCAGAAAAC
    TCCTACTTCT GCCAGGCAGC CCGACAGTTG GCATGCATCC CTTCCTCACA ACTGTGTGTC
    AAACTGGCAA GTGGAGGAGA CCCCACGTAT GCATTTAACA TCCGCTTTAC TGGAGAGGAG
    GTGCATGGTA CAAGTGGCTC ATTCCGTCAT TTCCTCTGGC AAGTGTGTAA AGAACTACAG
    AGCTCCTCGC TGTCTCTTCT ACTGCTCTGC CCCAGCTCTG CAGTTAATAA GAATAAGGGA
    AAATATCTTC TAACTCCCAG TCCCATAACA TATGCAGAAG AGCAGTTGCT CCATTTCTTT
    GGCCAACTAC TGGGAATAGC CATCAGAGCC GATGTTCCAC TCCCTCTGGA TCTACTCCCC
    TGCTTCTGGA AGATGCTTGT AGGAGAACCT TTGGACCCAG AAACTGATCT TTATGAAGCA
    GATATTCTCA CCCACAACTA CATCAAGAAG TTTGAAAATG TAAATGATGA GTCAGAGCTG
    GAAGCTCTTT GTGCTGAAAT TGCCTCTCAG CATCTTCCTA CCGAAAGTCC AGATTCTCCT
    CATAAACCAG CCTGCAAATT TACATACTTG ACAATGACTG GTGAAGAAGT GGAGCTGTGC
    CCTAAGGGAA GACACATTTC TGTAGTCTGG GAAAACAAAG ATATATATGC AATGGGAATC
    CGGAACCTTC GTATACGTGA GCTGAAGAAC CAGGAGTGTA TGAGCGCAGT AAGGGCTGGG
    CTGGGCTCCA TTATACCTCT GCAGCTCCTC ACCACTCTAA ATGCTCTAGA GATGGAACTA
    AGGACATGTG GCCTACCATA TATTAATTTG GAATTCTTAA AGGCTCACAC CATGTATCAA
    GTGGGTCTAA TGGAGACAGA TCAACACATT GAGTATTTTT GGTCTGCCTT AGAAATGTTC
    ACTCAGGAAG AACTTTGCAA ATTCATAAAA TTCGCCTGCA ATCAGGAACG CATTCCCTTT
    ACATGTCCAT GCAAAGATGG TGGGCCAGAC ACGGCTCATG TTCCACCTTA TCCTATGAAA
    ATAGCACCAC CCGATGGAGC AACAGGCTCC CCAGATTCCA GGTACATCCG AGTGGAGACA
    TGTATGTTCA TGATCAAGCT TCCGCAGTAC TCGTCTCTAG AGATGATGCT GGAGAAACTG
    AGATATGCCA TTCACTACAG GGAAGACCCA CTCAGCGGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa49025
    Clone ID Related Accession (Same CDS sequence) XM_012968794.3
    Accession Version XM_012968794.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13236bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product probable E3 ubiquitin-protein ligase HECTD4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012968794.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    301
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    601
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    841
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    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
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    2641
    2701
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    5881
    5941
    6001
    6061
    6121
    6181
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    6301
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    6421
    6481
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    7141
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    9061
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    9181
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    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
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    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGGGGTCGG CGGCTGCCGG GGACTCGGCT CAGTGGCTGT CGGTGAAAGA AGAGACTATC 
    TTTTTACATG ATGGGCTGAT CCGGGTCACA GACCTGGCGG AGTTGCCCAG CGAAATCGGG
    GTCAGTGAGG CCGGGGACGC AGAACTGGAG ATATTGACTT TTGAGACTAA GAACCCTATT
    GAGCTAGCAG AGCATTTACG TGCAGTGTGT GGGAACCAGA GCAATGCCTA TGCACGATTG
    CTGGAATACC GCTTGAATGC GTTACGTGGG TTATGGAATG CCCAACGCCA GCTGGCGCTT
    GAGGATCAAC ATGAAAGAGA TGGGGCTGAT GACGAAACCT TGGCCCTGCT TAAACGGCAG
    GGTTTGTTGC AACAGCCGGA ACAGGCTCCA TTTACATCTC GAATGGGGCT TTTACTGGTG
    TTCCCCCTTA TCCAGTCCCA AAGTAAAACA GACCCTTCAC TTTGTAACTT GACAGCTGAG
    GTGCTTTTAA ATTGCCTTCG GGACTGCCAA CCCCTGAGCT TGACTAAAGA ACCAGCTGAC
    TGCCTGAATG GAATAGAGTC CTTACTGTGT TCTTGGTTAG AAGACACTCC AAATGTGGGC
    CAACAAGTCT CCTTTAAGCA AAAGGAAAAT TCTGCTGCTG CTTTGGTTGC CTTAGCTTGT
    GCTAGGGGAT CATTAAAAAC ATTTATTCAC ACTGTACACT TGCTGCAAAA ACAGACTGAC
    CTAGGATCCC TTCCTGTGGC AGATGTATTG TATAGGTTGC TGCTTTTAGA GGGAGGTCCA
    GGTTCTCCCT CTTGTTTACT GGGAAGCAAA CACATTGTAT CCTGGGGTTT TGAGGATATG
    CTTCCAGCTC CTGATGGGAT CAGTGGTTCA AACAATGAGA ACAAAGATGC AGATTTGGGT
    CGCTGTGTAG CATGCGATGG GCTTTATCTT TACACAACTA ACTCTGTAGG TAGAGGATTG
    AGCAAAGTAG GGTCTGGGCT GCATGGCACT TTACGAGGTT TTGTTTATTC CCGAAACGAG
    GAGCTAGAGG CTGGCTGGAT TGCTTATGGA AGTGGGAAGA TACTTCACAG GCCTGCCTCC
    TTTGATAACA AACCTCAATA TCTTTTCCAA TTAATTGACC AATACACACT TCAGGTCTCT
    TATATAGTCA GCATGCCAGC CAATCATTTC CCGGTTGGCA GCAATATGAC TACGGTGCAC
    CTCTCCTCGG ATGGCACGTA TTTTTACTGG ATTTGGTCTC CAGCTAGTTT AAATGAGAAA
    ACCCCCAAAG GACACTCTGT CTTCATGGAT ATATTTGAGT TAAATGTGCA AAATGGAGTA
    TGCATTGCAA GCCCTCTTCA TGAGCGCATT ATATTAATGA GGAAAGAGGG AGAATCTGCT
    AAAAGTATTA ATGAAATGCT TTTGAGCAGA TTATCACGAT ACAGAGCTTC TCCTTCAGCA
    ACTCTAGCAG CACTTACAGG ATCTACAATC TCTAACACTC TAAAAGAGGA CCAGACTGCT
    GCAAATACGA GCTGCGGGCT TCCTTTGAAA ATGTTAAGAA AGACTCCAAT GTATACTTGT
    GGCACATACT TAGTACTACT CGTGCCACCT CCGGGTGGGT CAGGAAGTTC AGCAACAAGA
    TCTTTGTTTG GTGGAACCAG CGGCCTCTCC TCTTTAAAAA TTCTGGCTTC CAGCTTGGTA
    TTTAACATTT CTGATGGGCA GTTCACCAAC AGAGCAGATC TTATTGATGC ATCTGGAAGT
    TCTCTTGGAA GGGGTGCTTT GGTGACAGGC CTAGGTGCCT GCTATGATAC AATGAATAAC
    ATGATCTGGA CGTGCTCCAA TGATTACATC GATCAGTGGT GTAATCCTGG AAACCAGGCT
    TTCCATGTGG TGTGTCAGAG GCTTGGTGTC ACTCACATCA TTGCTGATCC CAAAGGAGAG
    GCAGTATCAA CAAATGAAGT TATAAACCAG TTGCTTCACC ATGTGGGTGC TATGTGTATT
    CACCAACTCA ATCTCCTGGC TAATAGCAAC AGTTCTCCAA TCAGTACCTT TCTGGGCAAA
    CAACATCCTA TAGAGCCACA TCATCTTAAT AGTATATGTG ACATCATGGA GAAAGCCATG
    GTCAATGGTG ATAAATGCAT TATTCGCTGC ATCTTGGTTG TTTTCCAGGT GGTATTTAAA
    TTCTTTCTAA ACCCACAAAC TGAGAGGAAT CGAGAAACCA TAAGGAGGGC TGGTTTGCTC
    CTTTGGCAGT TGCTCATGGC GCCTAAGGAC CAAATTTGTC CTGAAATACA GAGAGAGGTC
    TGCTTGGCTA TTAGCTTTGG TTTAAACATT TTGAATCCAG GAGAGCTAGA AATTAATAAC
    TTGCTCAAGC TGATTTTAAC TGAAGGGGAC AGAAATTGTG GCCTCTCCCA ACTCCGTGAT
    GTCATTTTGA CAAACCTCAC TGAACAGCTT CAGAATAACA GGTTTGGAAG TGAAGATGAT
    GAACATTACA GGCTTAATGA TGAACTATTG CACTATATTT TGAAGATTGT TGTTCGTGAA
    TCCTGTGTTC TGATCACCAA GTGCCAAACA GTGTCTAAAG AAGAATTCCA AAGACTCTTG
    TCAACAGTGC CTGTGGCTTC TCCCTGTCTA TGCTATTTAA TGGCTGTACA GAATCATCTC
    CTGAGTAACA CTGTCTTAAT TAAACCTGAT GACAGCGATG ACAGTGACAA CTCCCTGCAG
    GGGGAGACAT TGAAGGAACT CCAGGCCAGC ATCTTGGCTT TGGCAACCCA GATACTTTCT
    GGTTGTGATG AAGTTCTGGA TATTCTGCAG CGAGTGACCA CTGCTCTTAT CAACAGCGAC
    CTGTTAGACA GAGATTTAAG ACTGAAAGGC CTTGAACAGA TCACAAAGTC CACCCTGCTT
    GGCCATCTCC TCCCAGTACT TCTCACATCC CTTATGCATC CAAACTTACA GACATTAACC
    ATGGCAGATG CGCTTATGCC TCAGCTGGTG CAGCTTGTTC TTTACACTAG CCAGACTGCA
    CTGCTGCTTA AAACCCAGTG TCCAGTATTG GCAGATACAG GGGACACCAC CAGTGGAATT
    CCAGAGCAAA AAAACAAACT GTTAACTGAT GACAGGATGC TGGAAGAAAA GGAGGAGCCT
    GGTTTCCTCA CTGGGCTAAA GATTCCTGCG CCATGGGCTG CAGGAAAGAC TGTGGAAACA
    TCTCATCCAG TCAGAGACAA TTACAAGTTT AAAGAGACTG TGCACATTCC TGGAGCTCGT
    TGTTTGTACC TCCGGTTTGA CAGCAGGTGC TCTTCACAGT ACGATTATGA TAAGCTGGTG
    ATATATGCGG GACCCAACAC AAACAGCAGA AAAGTTGCCG AATATGGTGG AAATACCCTG
    GGGTATGGCA GTAGAAGTGT TTTAGGTACA GGCTGGCCAA AAGATTTGGT TAAGGTGGAA
    GGTGATACTG TGACATTCTC CTTCGAAATG AGAAGTGGGC GTGAGCACAA CACTCCTGAT
    AAAGCAATGT GGGGTTTTGC GTGCACTGTC AGAGCTCAGG AGTCCTCAGA AGATGTGTCA
    GGGGGCTTGC CATTTCTAGT AGACCTGGCT TTAGGTCTGT CTGTCTTGGC TTGTTCCATG
    CTGCGTATAT TGTACAATGG TCCAGAAATC ACAAAAGAAG AGTTGACTTG TCACGATCTG
    TTGAGGTCAA AGCTTTTGCA GAGGTGTCAG TGGCAGGTGG AGGCTAATGG TGCTTTATCC
    CCGGCACTCA CCCCAAGCCC TTCGCCGCTG CCCCTTACTA TAGAAGAAGA CCAAGAATTT
    ACGTATCCAT CTGACATCTT GTTGCCACCA GTTGGGAACT ATTTTGAACT GCCAAGAATC
    AGACTGCCTC CTGGTATTAT GATCAAGCTC AGGGAAATAT CTGGACGCGC TCGACCACAG
    TTTAGACCAA GCATAAAAGA GGTTATCCAG CCTGATGTGA TGGAAGAAAT GGTTGTATCT
    TGCGTCATTA AACACTTGAA CCTGGTCGAT GCATTGCAGT CTTTAATAAA CTTCCAGTAT
    CGAGAAGAAC ATCTAGAAGA ATATGATTTA TTATGTAAAA TAATGGGGGA AACTTTTAAG
    AAGCTCAATG CAATGGAAAG ACAATTGCAG AGTGTTGCAG AACTGGAACA AAAGTGGCAA
    AGTGAAGTGG AAGATGCTCA GAATGGAAAG CTGGAGAACA ACGCTCCCTT TTTTTACGAC
    TATCATTTTA ATGAGAGCAA GTTAAAGGAA CTTGAACTTT TATGTTCTAT GAAAGAGGTC
    CCTTCTGATG GAAGTGATCC AGAAAACATG GTTTTCTCTC TGCGAGAGAA GTTTTTTCAA
    GAGGTGAACT CCCTGATCCA AAAACCTTCC CACCCAATGG CTAAGACCAA AACATTGGTT
    AAGAGTCTGA TGAATAGAGC AGAGCTCCTG TTACATGTGA CTATTGCAGC ACATTCTGGT
    ATCACGAGAA GCATATCTGG CACTCCAACT GAACCTCAAG CTGGCAAGTC TATCTCAGAA
    GCAAAAGTTA TATCTCAAGC CATGCGCCAG CCTGTTTTCC TCCGCAGCAT GTCTGCCCCT
    TCTGACCTGG AAATGATTGG AAGTAGTGAC TTAGAGTTCA GCAGGTCTAA TCCAAGGAGA
    AGGAACATCA CTAGCCACAG GAGCAGCTCC TTCACTCTGC TTCAGTCTTT AGCTATTGAA
    GACTGCAAGG ATAAACCAAC ATACAGTGTC CTTTTAGGGC AACTGTTTGC CTTCATTGGG
    ACCAATCCCG ACCAAGCAGT ATCCAGTAGC AGCTTTCTAC TTGCAGCCCA AACCAGATGG
    CGTCGTGGTA ATACTCGCAA ACAAGCCCTT GTACACATGA GAGAATTACT GACTGCTGCT
    GTCCGTGTTG GGGGTGTGAC ACATTTGGTT GGACCAGTAA CAATGGTGCT ACAGGGAGGA
    CCAAGAATTG AAGAGCTCAC CTGTGGAGGA ATGGTGGAGC TTGTTCAAGA GGCATTTGGA
    GAGACCATGA CCTCAGTGGT GTCACTTTGT GCACGGTATC CCATTGCCTG TGCAAATAGC
    ATTGGGCTTT TGTGTACTAT ACCTTACACA AGGAGTGAAG AAAAATGTTT AGTTCGCAGT
    GGGCTTGTTC AACTGATGGA CCGACTCTGC AGCTTAAGCA ACCAGAGAGA GTCCAGCTCA
    AATGAAAAGC AAACCAAGAA ACAGAAAGTA GCAACTATGG CATGGGCAGC CTTCCAAGTA
    CTGGCCAATA GATGTGTAGA ATGGGAGAAA GAAGAAGGTG GTTCTACTGA GGCGGTACAC
    TCTGGTTTGG CTAGACAAGT CTCCACCCTC CTCACTAACC ATCTAGCAAG GGCTACAGAG
    TGCTGTGGAA ACCAAGCTGC AGGGAATGAT GCCCTACAGG ACGTTCTGAG CCTTCTCAAT
    GATCTCTCTA GGAGTCAGAT AGGGAAGGCA ATTCTGAGCC AGCCTGCTTG TGTGTCCAAA
    CTTCTTTCGT TGCTGCTGGA CCAGCGTCCT TCTCCAAAGC TTGTGCTCAT AATTCTGCAG
    CTGTGCCGCG CTGCACTTCC TCTCATGAGT GTTGAAGACT GTGGAAATGT AGAACTTCCT
    CCATGGAGTT ACTCTTTACC AGCTCTGGAC AGTGAACAGG GGGAGGATCC AAGTGACCCA
    GCCTCCAAAA TAGCATCTTT GTTGCTAGCA AAGTTGGCTG ATTATGTTGT TCCAGGATGT
    CAGACAGTTC TTTCTCCTAC TGCTTCTGAA CCAGACAGCT CATTGTCCAA AAACAGCCCA
    AAAAATTCCC AGAAAGCAGA TAAGGATGCA GAAGAAGAGA GTGAAGCAGT AGATGGCAAG
    CTCTCCATTT TTATTCATAA GCGAGAAGAC CAGTCATCCC ATGAGGTTCT TCAGCCTTTG
    CTTAGTAGTT CAGAGGGAAG GCCTTTTCGT CTTGGTACTG GAGCGAATAT GGAAAAAGTA
    GTAAAGATGG ATCGTGACAT GACTAAGAGT GGATGTTGTG AAGTAATCAC AGAGGAGGCT
    GCAGCTGCTC TCCGCAAAGC CACTAAATGG GCCCAGTCGG GTTTAATTGT TAGCATTGGG
    CCACCTGTGG AGACAGTTAG CCCTGATTCA GCGAGTGGAC TTTCCACTGG TGACAAAAAG
    AAAACAGCCC AGACCTCGAT CTGTAGGGAA AGGAATTCTG AACTTGCCAG GACTGATCCT
    GTACGCCCTT TCATTAGTGG CCATGTAGCA AACAGCATGG CTGCAGAAGT AATTGCTCTG
    CTTCACAGCC TGCTGATGGC ACCAGAGTCA AATGCTGCCC AAATCTGGAC AACAACAGCA
    GAGAAGGTTC TTTCTCGTGC CCTGATGTAT ATCCCACAGC TTGGCAAATA TGCAGAAAGC
    ATCCTAGAGA ACGGCAGCGG TAATGGCAGG AAGCTAGCAA AGCTACAAGG CATTGCAAGG
    CAAGCAGTTG CTGCATTATG TGCGCTTGGT GGATTTAAAG AAACCATTAA AATAGGTTCT
    GAAGTCCAGG TGTTGGGTAA AGGAATATCT GGGAGCATTG GAGTGGTTGT TTCTATTAAT
    GAACAAGAAG GTATTGCAAC AGTGAAATTT CCAGACATAG ATTTTAGAAA ATCATGCAAA
    GCATCTGACA CACTGACAGT ACCATTATTT AGGCTCTGCG TTCCACGGTC AGAGGCATTG
    CCTCTCCATA AACTGTCCAT TACTGAGAAG GTAGTGCAGG CAGTCCAGTC CATGTTACTT
    CCCCAGGAGG GCTGCCTATC TATTCATACC TCGCTTCCTG CAACAGGAGA TGCCTCAACT
    CCAGTAATGG CAGTGGTTCG ACTTCTTGCA GAGATCAGGA CAAGAGCTTG CTTGGTGATG
    GCTCAGCTGT TGGAGGACAG CTCATTTTGT GAAGAATTCA TACAGCAATG TCCAGCTGCT
    GTAGAAGTTC TAAACCTAGT GGCCCAGGAA TGCAGTCCTG GGGAGCGTCT CTCAGTGGTG
    GAAGTTCAGT GTGAAAGACT ACGAATGCTT TATCGGGACT GTGCTCGCCC CCCGCCACCA
    CCTTTGCAGG CTGAGCGCAG ACAGCCCAAG GAAATCACCT GGTCTCCTTC CAGGGTATTC
    CCTCCTGTGA GAGCCTGTAT GTTTTCATCC CATCTAACAG CAGTAACATT TCTGGCTGAC
    CCAAGTGCTG GTGGGGGACT TCCTCGCGGC ACATTTATAT ATGCAACATC CCCTGTTCCA
    GTACAGGCAC CATCTTTTTA CTGGGAAATA GAAGTAGTTT CCTATGGAGA CACAGATGAT
    GACACTGGCC CTATTGTATC ATTTGGGTTT GCTACAGAAG CAGAGAAGCG GGATGGAGCA
    TGGACAAACC CAGTCGGTAC CTGTCTTTTT CATAACAATG GTCGAGCTGT ACATTACAAT
    GGATCAAGTC TCTTACAGTG GAAGAGTGTT CGATTAGATG TGACTCTTGC TCCTGGTGAT
    GTTGCTGGAA TAGGTTGGGA GAGAACTGAA GGAACACCAC CACCCCCAGG GCAGCCTGCC
    AAGGGCAGAG TCTACTTTAC TTACTGTGGA CAGAGGCTTA CTCCGTTCTT AGAGGATGTC
    TCTGGGGGCA TGTGGCCAGT TGTGCATATT CAGAAAAAGA ACACTAAAGT CCGTGCAAAT
    TTTGGATGCA GACAGTTTGC TTTTGCCGAG GGTCAAGCTC ATCGCAATGC AGCAGATTTG
    TGCATTGATT TGGCTGAAGA GATAAGTGCA AACTTTGAAG CATTGCCCTT TGCTATGGCA
    TCTGACAGTG ATAATGATGC TGGGACAAGC ATAGCATCTG ATCCTGGAAC TCATGGACCT
    CCTTGCCGTA TTGCTGCAGT TTCTACTGTG CAACAGCAAT ATGACAGTGA TGCCTCATGC
    CGTTACAAGG TAGAGCTGAG TTATCAAGAC TTTGTGACCT CTGGACCTGA TCCACACCCA
    CCTCCCATTG CAGATGATGA AAGTGATGAT GATGACGATG ATGATATTCC TCGAGAGGAT
    CATTATGCTC TTTTGGTAAA AGCATGGGAG ACCAAAGTTT TTCCAACCAT TCGAAGACGT
    TTCCGTAACG AGGCAGAGAG AAAGTCTGGC CTGGACCAGA TTAAGGGAGC TCTTCAGCTA
    GGAATGGTGG ATATAGCGAG GCAAACTGTA GAGTTCCTCT ATGAAGAAAA TGGTGGCATT
    CCAAGAGATC TCTATCTTCC TACTATTGAG GATATCAAGG ATGAGGCAAA TAAATTCACC
    ATTGATAAAG TTCGCAAAGG ACTCACAGTG GTCATTCGAT CCCCAGATAG CAACAATGTA
    GCTGGGACAA CTGTGGGAAC AGCTTTACCC AAGTTTGCCA TCCGTGGTAT GCTGAAGACA
    TTCTCTCTAC ATGGGGTGGT TCTGGATGTG GATTCAGTTA ATGAGCTGGT TCAGGTAGAG
    ACTTACCTCC GGAGTGAAGG GGTATTAGTG AGATACTGGT ATCCCATAGA TATGCTAGAA
    AGACCTCCTG CGGGATACAG GAGAATGGCA ACCAATGGAC TGGTGACTTT AGATAGCACT
    AACATCCAGA TTCATAGAGA GCTGTTGCGG TGTGAGTCTG CACTTGCCAG GCTTTACTGT
    CGTGTGGCTT TGCTTAACAT CTTTGCTCCT AAGTCTCCGC ACACTTTCAC TCGCCTGTTC
    CATATTCCTG CTATCCGAGA CATTACATTG GAGCATTTAC AGCTTCTGTC AAATCAGCTT
    CTAATGCCAC CCCTTCCAGA CGGAACCATT ACCTCCAGCT CTATCCTTCT TGCCCAGTCC
    TTGCAACATT GCATCTACTC CCAGAACTGC TCAACAACAG ATTTGTTCTA CCAGGGCAAT
    GCACAGCCAA TAAGAGAGTG GCTTGGTGTG GCCATAACCA GGGCCCTGCA TCAAGGAGAG
    GACAGTCTCT TGGATCTGAC AAAACAAATC TGTTCCTTCT TGCAAAATGC GCCGGAACAG
    TTTCCATCTG AAGAATTTCC CATCTCTGAA TCCAAGCTCA GTATGGATGT TAATTTTCCT
    GGGGCAGCTT TTGTTATTGT TTCTTGTATA GAGAGTCCGT CTGGATTTAG AAAAGACTCC
    TCCTTGTACA AGGCTCCCTG GTCTAGAGTC ATGGTATATG GCCTTGGACA TAAAGTAAAG
    AGGAATGGAC AGCTGAACCT GATAGAATCA GTTTGTTATC CACGTGATGC CTCCCCAAGT
    AACACAGGCC TCACTCCCCC ACCAACCACA AACCAGTACC CAACAGCGAT TATTCCCACT
    GACAAGGTCC ACGTCAAACT TGGTGTATCA CCTACTCCTG GTGCTGTGCT GGTCATCCAT
    TCCCTGCCTC TAGAGTTTCC GTTGGCAATG GCATTCACAG AACAGCTGTT AACCTGGAAG
    TTGGAGGACA CAGAAGAGAA GTCAGAAGAT GAGCTTGATA CTGTTCCATC CTCTGCTCTG
    TTGCAAGTGG TAGAACTTTT AGGTACTTTG ATTTGGACAA CAGACCTTGC TGCAGTAATT
    AAGGAGCTTC TGTTCCATCT GTTGGCTGAA CTATTAAGGA AAGTTCACCA TCTAGAGCAG
    AAGAAGAACC CAAGTGGATT ATCTTCCTCG ATAGCCCTGC AGTTGAATCC ATGTCTAGCA
    ATGCTTATGG CTTTACAGAC AGAACTGCGC AAGCTGTATG ATGAAGAAAC TCAAAACTGG
    GCATCTGGGA ATGCATGTGG GGGGTCTGTA CCTGGAGCCT CTGAGCAGGG AAGATTTTCA
    ACTTACTTTC ATGCCCTCAT GGAAGTGTGC CTGGCAGTGG CAGAAGTGAC GCTCCCAATC
    AACATGAGCT CAGCAATCAG TGTGATGGCA TCTACAAATG CCACTAATCT GAGTGACTCT
    TCTTCTTCCT CTTCCTCTTC CCCTGGACAG ACTCCTCAAA GTCCAAGTCT GTTATCAAAG
    AGAAAGAAAG TTAAGATCAA ACGTGAGAAG ACTGCATCTG GAAAACGTAC ACCTTCACGT
    GGTGCAGAGA GTGATGCTGC TTTGCTTAGC ATTGGAGGAA GCAAACCTGA GGACATGTTG
    TGGTTCCATA GGGCATTGAC TTTATTGATG ATCCTAAGGC ATCTAACTAA CAAGGATCCA
    CAGGGGATGG GTGTCACAAA CGATGCTATT GCTGATGCTA GCCAGGCTTT AGTTGGTCCC
    ACAGCTCACA GTCGTCTCCT TGTGATTTCT GGCATCCCTA CACACCTAGA AGAATCAGTG
    GTCAGAAGTG CAATTAGAAA GGCATGTAAT GCTCATGGTG GAGTCTTTAA AGATGAAATC
    TACATTCCAT TGCAGGAAAC TGAAACCCCT AAACCCAAAG ACAGAATAGA AGGGGCAGAA
    TGCAAGACAG AAATAGAAAA ACCCTCTACG CAGCATACAA CCGACAATAT GGATATCAGC
    AGCTCGAGCA GTGTAACTCC TGCAGCAAGT GTCACGGCAT CAGCTTCTAC AAGCCAAGCT
    TCATTGTGCA GCTCCCAAGG GGGGGTATCA CGGACCACAA GTGAAACATC TATGGAGCAG
    TTTGCCCCAG AACATGCTGT GCCATCAGGG CTTCTGGAAC CAACTATTAT CTCCAGCCAG
    GAGAGTCTGG ATCTTTCTAT GTGCAGCACA GGAAGCCTCG GCAGTCTGGG AAGTCTTGTT
    GAACAGCTTG ATAATGTTGA AAACTCTTTG TCTGACGTGG GCTCCCTATA CACAGCTACA
    TCACTAGATA GCCATATGGC AGAGTCGCGG CCAATCAAAG GATTTGCAGT TGTAGAGGTG
    CGATCACGAG CTAAAATAGA GAAAATCCGT GCCACTTTAT TTAACAGTAG TGACCTCATT
    GGGTTATCTG GTTTGGATGG TGAAGATGAG CTGATGGAAA TGTCTACTGA GGAGATCTTG
    ACTGTTTCAA CAGTGAATCA GAGCTTGTTT GATACCCAAG GAATACCCGG TCTGGCTGAT
    TATTTCATGG ATAAATCAAT GAAAGGTGAT AAATTAGTCC CGGAAGCTCG TGATGTTCTG
    ACTGAGATTT TCAAGAGCTG CATTCACGCC GAGCAGATGC TGAGTCTGAC TCCAGCCAAG
    TTTATCAAAG TGTCTGATAT CTACCTTAGC AAGGAGCAAA TCAATTCACA GACCCCAGGG
    AACTTACTGC ATACCTTTTT CACCAATGTT CGACCACCTA AAAAAGTCCT GGAAGATCAA
    CTAACGCAGA TCTTAAGAAA ATATGGAATT CCCAAGCCTA AATTTGACAA AAGCAAATAC
    AGCAAAACTG GGAAGGAGCA ACATCCTGTG AAAGTTGTAA GCACCAAAAG ACCTGTGGGC
    AAACCACCGA CCAAAGACAA GGCTGTATTC AACAGCGTCA GGACTGCTTT AAGTGAGAAA
    AAACCAACTG TGAAGCCAAA ATCTCCGGAG AAGGGCAGGC CAGATGAGAA GGACCCAGAC
    AAATCTCCAA CAAAGAAGCA AGAAAGGTTT CACTTGTGTC CCTATCTAGT TCCCGAGGAG
    AGATATCTGA CGTTGGATGG ATTTCACCGC TTTGTAGTAG ATCGGGCTAA GCAAGACATT
    CGAAGCATCT GGAGAGCTGT CTTGGCGTGT GGATATGATC TTCATTTTGA AAGGTGTGCC
    TGCATTGATA CAAGGCATGC TCAGAAATCT AGCAGAAAAT GGACCTTGGA AATGGATGTT
    GCTCTTGTTC AGTATATCAA CCGCCTTTGC CGTCACCTTG CCATAACGCC AGCCCGACTA
    CACCCTCATG AGGTTTATCT GGATCTTGCA GATACGGCTG ACCCAAGAGT CTCCTGCCTG
    CTGAATGTAC CAATAGAAAG CCTGCGCCTC CGATTTGCTC TTCTTCAGTC CCTCAACACT
    ACTTTAGAGA CCTTTTTCCT GCCCTTAGTG GAGCTACGGC AAACAGACGT ATACACACAC
    AGCATCGCAT CCCTACTCCA GCTAGCTAAA GGGTTAATCT TTTACGATAC AAAAGTGAAT
    GTGATGAACC GGGTATTGAA CGCAACAGTG CAGAGGACAG CAGATCATGC AGCCCCTGAG
    ATCACGCTGG ACCCACTAGA AATTGTAGGA GGGGAAATTC GTACCTCAGA AAACTCCTAC
    TTCTGCCAGG CAGCCCGACA GTTGGCATGC ATCCCTTCCT CACAACTGTG TGTCAAACTG
    GCAAGTGGAG GAGACCCCAC GTATGCATTT AACATCCGCT TTACTGGAGA GGAGGTGCAT
    GGTACAAGTG GCTCATTCCG TCATTTCCTC TGGCAAGTGT GTAAAGAACT ACAGAGCTCC
    TCGCTGTCTC TTCTACTGCT CTGCCCCAGC TCTGCAGTTA ATAAGAATAA GGGAAAATAT
    CTTCTAACTC CCAGTCCCAT AACATATGCA GAAGAGCAGT TGCTCCATTT CTTTGGCCAA
    CTACTGGGAA TAGCCATCAG AGCCGATGTT CCACTCCCTC TGGATCTACT CCCCTGCTTC
    TGGAAGATGC TTGTAGGAGA ACCTTTGGAC CCAGAAACTG ATCTTTATGA AGCAGATATT
    CTCACCCACA ACTACATCAA GAAGTTTGAA AATGTAAATG ATGAGTCAGA GCTGGAAGCT
    CTTTGTGCTG AAATTGCCTC TCAGCATCTT CCTACCGAAA GTCCAGATTC TCCTCATAAA
    CCAGCCTGCA AATTTACATA CTTGACAATG ACTGGTGAAG AAGTGGAGCT GTGCCCTAAG
    GGAAGACACA TTTCTGTAGT CTGGGAAAAC AAAGATATAT ATGCAATGGG AATCCGGAAC
    CTTCGTATAC GTGAGCTGAA GAACCAGGAG TGTATGAGCG CAGTAAGGGC TGGGCTGGGC
    TCCATTATAC CTCTGCAGCT CCTCACCACT CTAAATGCTC TAGAGATGGA ACTAAGGACA
    TGTGGCCTAC CATATATTAA TTTGGAATTC TTAAAGGCTC ACACCATGTA TCAAGTGGGT
    CTAATGGAGA CAGATCAACA CATTGAGTAT TTTTGGTCTG CCTTAGAAAT GTTCACTCAG
    GAAGAACTTT GCAAATTCAT AAAATTCGCC TGCAATCAGG AACGCATTCC CTTTACATGT
    CCATGCAAAG ATGGTGGGCC AGACACGGCT CATGTTCCAC CTTATCCTAT GAAAATAGCA
    CCACCCGATG GAGCAACAGG CTCCCCAGAT TCCAGGTACA TCCGAGTGGA GACATGTATG
    TTCATGATCA AGCTTCCGCA GTACTCGTCT CTAGAGATGA TGCTGGAGAA ACTGAGATAT
    GCCATTCACT ACAGGGAAGA CCCACTCAGC GGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012824248.1
    CDS321..13556
    Translation

    Target ORF information:

    RefSeq Version XM_012968794.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 4 (hectd4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012968794.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGGGGTCGG CGGCTGCCGG GGACTCGGCT CAGTGGCTGT CGGTGAAAGA AGAGACTATC 
    TTTTTACATG ATGGGCTGAT CCGGGTCACA GACCTGGCGG AGTTGCCCAG CGAAATCGGG
    GTCAGTGAGG CCGGGGACGC AGAACTGGAG ATATTGACTT TTGAGACTAA GAACCCTATT
    GAGCTAGCAG AGCATTTACG TGCAGTGTGT GGGAACCAGA GCAATGCCTA TGCACGATTG
    CTGGAATACC GCTTGAATGC GTTACGTGGG TTATGGAATG CCCAACGCCA GCTGGCGCTT
    GAGGATCAAC ATGAAAGAGA TGGGGCTGAT GACGAAACCT TGGCCCTGCT TAAACGGCAG
    GGTTTGTTGC AACAGCCGGA ACAGGCTCCA TTTACATCTC GAATGGGGCT TTTACTGGTG
    TTCCCCCTTA TCCAGTCCCA AAGTAAAACA GACCCTTCAC TTTGTAACTT GACAGCTGAG
    GTGCTTTTAA ATTGCCTTCG GGACTGCCAA CCCCTGAGCT TGACTAAAGA ACCAGCTGAC
    TGCCTGAATG GAATAGAGTC CTTACTGTGT TCTTGGTTAG AAGACACTCC AAATGTGGGC
    CAACAAGTCT CCTTTAAGCA AAAGGAAAAT TCTGCTGCTG CTTTGGTTGC CTTAGCTTGT
    GCTAGGGGAT CATTAAAAAC ATTTATTCAC ACTGTACACT TGCTGCAAAA ACAGACTGAC
    CTAGGATCCC TTCCTGTGGC AGATGTATTG TATAGGTTGC TGCTTTTAGA GGGAGGTCCA
    GGTTCTCCCT CTTGTTTACT GGGAAGCAAA CACATTGTAT CCTGGGGTTT TGAGGATATG
    CTTCCAGCTC CTGATGGGAT CAGTGGTTCA AACAATGAGA ACAAAGATGC AGATTTGGGT
    CGCTGTGTAG CATGCGATGG GCTTTATCTT TACACAACTA ACTCTGTAGG TAGAGGATTG
    AGCAAAGTAG GGTCTGGGCT GCATGGCACT TTACGAGGTT TTGTTTATTC CCGAAACGAG
    GAGCTAGAGG CTGGCTGGAT TGCTTATGGA AGTGGGAAGA TACTTCACAG GCCTGCCTCC
    TTTGATAACA AACCTCAATA TCTTTTCCAA TTAATTGACC AATACACACT TCAGGTCTCT
    TATATAGTCA GCATGCCAGC CAATCATTTC CCGGTTGGCA GCAATATGAC TACGGTGCAC
    CTCTCCTCGG ATGGCACGTA TTTTTACTGG ATTTGGTCTC CAGCTAGTTT AAATGAGAAA
    ACCCCCAAAG GACACTCTGT CTTCATGGAT ATATTTGAGT TAAATGTGCA AAATGGAGTA
    TGCATTGCAA GCCCTCTTCA TGAGCGCATT ATATTAATGA GGAAAGAGGG AGAATCTGCT
    AAAAGTATTA ATGAAATGCT TTTGAGCAGA TTATCACGAT ACAGAGCTTC TCCTTCAGCA
    ACTCTAGCAG CACTTACAGG ATCTACAATC TCTAACACTC TAAAAGAGGA CCAGACTGCT
    GCAAATACGA GCTGCGGGCT TCCTTTGAAA ATGTTAAGAA AGACTCCAAT GTATACTTGT
    GGCACATACT TAGTACTACT CGTGCCACCT CCGGGTGGGT CAGGAAGTTC AGCAACAAGA
    TCTTTGTTTG GTGGAACCAG CGGCCTCTCC TCTTTAAAAA TTCTGGCTTC CAGCTTGGTA
    TTTAACATTT CTGATGGGCA GTTCACCAAC AGAGCAGATC TTATTGATGC ATCTGGAAGT
    TCTCTTGGAA GGGGTGCTTT GGTGACAGGC CTAGGTGCCT GCTATGATAC AATGAATAAC
    ATGATCTGGA CGTGCTCCAA TGATTACATC GATCAGTGGT GTAATCCTGG AAACCAGGCT
    TTCCATGTGG TGTGTCAGAG GCTTGGTGTC ACTCACATCA TTGCTGATCC CAAAGGAGAG
    GCAGTATCAA CAAATGAAGT TATAAACCAG TTGCTTCACC ATGTGGGTGC TATGTGTATT
    CACCAACTCA ATCTCCTGGC TAATAGCAAC AGTTCTCCAA TCAGTACCTT TCTGGGCAAA
    CAACATCCTA TAGAGCCACA TCATCTTAAT AGTATATGTG ACATCATGGA GAAAGCCATG
    GTCAATGGTG ATAAATGCAT TATTCGCTGC ATCTTGGTTG TTTTCCAGGT GGTATTTAAA
    TTCTTTCTAA ACCCACAAAC TGAGAGGAAT CGAGAAACCA TAAGGAGGGC TGGTTTGCTC
    CTTTGGCAGT TGCTCATGGC GCCTAAGGAC CAAATTTGTC CTGAAATACA GAGAGAGGTC
    TGCTTGGCTA TTAGCTTTGG TTTAAACATT TTGAATCCAG GAGAGCTAGA AATTAATAAC
    TTGCTCAAGC TGATTTTAAC TGAAGGGGAC AGAAATTGTG GCCTCTCCCA ACTCCGTGAT
    GTCATTTTGA CAAACCTCAC TGAACAGCTT CAGAATAACA GGTTTGGAAG TGAAGATGAT
    GAACATTACA GGCTTAATGA TGAACTATTG CACTATATTT TGAAGATTGT TGTTCGTGAA
    TCCTGTGTTC TGATCACCAA GTGCCAAACA GTGTCTAAAG AAGAATTCCA AAGACTCTTG
    TCAACAGTGC CTGTGGCTTC TCCCTGTCTA TGCTATTTAA TGGCTGTACA GAATCATCTC
    CTGAGTAACA CTGTCTTAAT TAAACCTGAT GACAGCGATG ACAGTGACAA CTCCCTGCAG
    GGGGAGACAT TGAAGGAACT CCAGGCCAGC ATCTTGGCTT TGGCAACCCA GATACTTTCT
    GGTTGTGATG AAGTTCTGGA TATTCTGCAG CGAGTGACCA CTGCTCTTAT CAACAGCGAC
    CTGTTAGACA GAGATTTAAG ACTGAAAGGC CTTGAACAGA TCACAAAGTC CACCCTGCTT
    GGCCATCTCC TCCCAGTACT TCTCACATCC CTTATGCATC CAAACTTACA GACATTAACC
    ATGGCAGATG CGCTTATGCC TCAGCTGGTG CAGCTTGTTC TTTACACTAG CCAGACTGCA
    CTGCTGCTTA AAACCCAGTG TCCAGTATTG GCAGATACAG GGGACACCAC CAGTGGAATT
    CCAGAGCAAA AAAACAAACT GTTAACTGAT GACAGGATGC TGGAAGAAAA GGAGGAGCCT
    GGTTTCCTCA CTGGGCTAAA GATTCCTGCG CCATGGGCTG CAGGAAAGAC TGTGGAAACA
    TCTCATCCAG TCAGAGACAA TTACAAGTTT AAAGAGACTG TGCACATTCC TGGAGCTCGT
    TGTTTGTACC TCCGGTTTGA CAGCAGGTGC TCTTCACAGT ACGATTATGA TAAGCTGGTG
    ATATATGCGG GACCCAACAC AAACAGCAGA AAAGTTGCCG AATATGGTGG AAATACCCTG
    GGGTATGGCA GTAGAAGTGT TTTAGGTACA GGCTGGCCAA AAGATTTGGT TAAGGTGGAA
    GGTGATACTG TGACATTCTC CTTCGAAATG AGAAGTGGGC GTGAGCACAA CACTCCTGAT
    AAAGCAATGT GGGGTTTTGC GTGCACTGTC AGAGCTCAGG AGTCCTCAGA AGATGTGTCA
    GGGGGCTTGC CATTTCTAGT AGACCTGGCT TTAGGTCTGT CTGTCTTGGC TTGTTCCATG
    CTGCGTATAT TGTACAATGG TCCAGAAATC ACAAAAGAAG AGTTGACTTG TCACGATCTG
    TTGAGGTCAA AGCTTTTGCA GAGGTGTCAG TGGCAGGTGG AGGCTAATGG TGCTTTATCC
    CCGGCACTCA CCCCAAGCCC TTCGCCGCTG CCCCTTACTA TAGAAGAAGA CCAAGAATTT
    ACGTATCCAT CTGACATCTT GTTGCCACCA GTTGGGAACT ATTTTGAACT GCCAAGAATC
    AGACTGCCTC CTGGTATTAT GATCAAGCTC AGGGAAATAT CTGGACGCGC TCGACCACAG
    TTTAGACCAA GCATAAAAGA GGTTATCCAG CCTGATGTGA TGGAAGAAAT GGTTGTATCT
    TGCGTCATTA AACACTTGAA CCTGGTCGAT GCATTGCAGT CTTTAATAAA CTTCCAGTAT
    CGAGAAGAAC ATCTAGAAGA ATATGATTTA TTATGTAAAA TAATGGGGGA AACTTTTAAG
    AAGCTCAATG CAATGGAAAG ACAATTGCAG AGTGTTGCAG AACTGGAACA AAAGTGGCAA
    AGTGAAGTGG AAGATGCTCA GAATGGAAAG CTGGAGAACA ACGCTCCCTT TTTTTACGAC
    TATCATTTTA ATGAGAGCAA GTTAAAGGAA CTTGAACTTT TATGTTCTAT GAAAGAGGTC
    CCTTCTGATG GAAGTGATCC AGAAAACATG GTTTTCTCTC TGCGAGAGAA GTTTTTTCAA
    GAGGTGAACT CCCTGATCCA AAAACCTTCC CACCCAATGG CTAAGACCAA AACATTGGTT
    AAGAGTCTGA TGAATAGAGC AGAGCTCCTG TTACATGTGA CTATTGCAGC ACATTCTGGT
    ATCACGAGAA GCATATCTGG CACTCCAACT GAACCTCAAG CTGGCAAGTC TATCTCAGAA
    GCAAAAGTTA TATCTCAAGC CATGCGCCAG CCTGTTTTCC TCCGCAGCAT GTCTGCCCCT
    TCTGACCTGG AAATGATTGG AAGTAGTGAC TTAGAGTTCA GCAGGTCTAA TCCAAGGAGA
    AGGAACATCA CTAGCCACAG GAGCAGCTCC TTCACTCTGC TTCAGTCTTT AGCTATTGAA
    GACTGCAAGG ATAAACCAAC ATACAGTGTC CTTTTAGGGC AACTGTTTGC CTTCATTGGG
    ACCAATCCCG ACCAAGCAGT ATCCAGTAGC AGCTTTCTAC TTGCAGCCCA AACCAGATGG
    CGTCGTGGTA ATACTCGCAA ACAAGCCCTT GTACACATGA GAGAATTACT GACTGCTGCT
    GTCCGTGTTG GGGGTGTGAC ACATTTGGTT GGACCAGTAA CAATGGTGCT ACAGGGAGGA
    CCAAGAATTG AAGAGCTCAC CTGTGGAGGA ATGGTGGAGC TTGTTCAAGA GGCATTTGGA
    GAGACCATGA CCTCAGTGGT GTCACTTTGT GCACGGTATC CCATTGCCTG TGCAAATAGC
    ATTGGGCTTT TGTGTACTAT ACCTTACACA AGGAGTGAAG AAAAATGTTT AGTTCGCAGT
    GGGCTTGTTC AACTGATGGA CCGACTCTGC AGCTTAAGCA ACCAGAGAGA GTCCAGCTCA
    AATGAAAAGC AAACCAAGAA ACAGAAAGTA GCAACTATGG CATGGGCAGC CTTCCAAGTA
    CTGGCCAATA GATGTGTAGA ATGGGAGAAA GAAGAAGGTG GTTCTACTGA GGCGGTACAC
    TCTGGTTTGG CTAGACAAGT CTCCACCCTC CTCACTAACC ATCTAGCAAG GGCTACAGAG
    TGCTGTGGAA ACCAAGCTGC AGGGAATGAT GCCCTACAGG ACGTTCTGAG CCTTCTCAAT
    GATCTCTCTA GGAGTCAGAT AGGGAAGGCA ATTCTGAGCC AGCCTGCTTG TGTGTCCAAA
    CTTCTTTCGT TGCTGCTGGA CCAGCGTCCT TCTCCAAAGC TTGTGCTCAT AATTCTGCAG
    CTGTGCCGCG CTGCACTTCC TCTCATGAGT GTTGAAGACT GTGGAAATGT AGAACTTCCT
    CCATGGAGTT ACTCTTTACC AGCTCTGGAC AGTGAACAGG GGGAGGATCC AAGTGACCCA
    GCCTCCAAAA TAGCATCTTT GTTGCTAGCA AAGTTGGCTG ATTATGTTGT TCCAGGATGT
    CAGACAGTTC TTTCTCCTAC TGCTTCTGAA CCAGACAGCT CATTGTCCAA AAACAGCCCA
    AAAAATTCCC AGAAAGCAGA TAAGGATGCA GAAGAAGAGA GTGAAGCAGT AGATGGCAAG
    CTCTCCATTT TTATTCATAA GCGAGAAGAC CAGTCATCCC ATGAGGTTCT TCAGCCTTTG
    CTTAGTAGTT CAGAGGGAAG GCCTTTTCGT CTTGGTACTG GAGCGAATAT GGAAAAAGTA
    GTAAAGATGG ATCGTGACAT GACTAAGAGT GGATGTTGTG AAGTAATCAC AGAGGAGGCT
    GCAGCTGCTC TCCGCAAAGC CACTAAATGG GCCCAGTCGG GTTTAATTGT TAGCATTGGG
    CCACCTGTGG AGACAGTTAG CCCTGATTCA GCGAGTGGAC TTTCCACTGG TGACAAAAAG
    AAAACAGCCC AGACCTCGAT CTGTAGGGAA AGGAATTCTG AACTTGCCAG GACTGATCCT
    GTACGCCCTT TCATTAGTGG CCATGTAGCA AACAGCATGG CTGCAGAAGT AATTGCTCTG
    CTTCACAGCC TGCTGATGGC ACCAGAGTCA AATGCTGCCC AAATCTGGAC AACAACAGCA
    GAGAAGGTTC TTTCTCGTGC CCTGATGTAT ATCCCACAGC TTGGCAAATA TGCAGAAAGC
    ATCCTAGAGA ACGGCAGCGG TAATGGCAGG AAGCTAGCAA AGCTACAAGG CATTGCAAGG
    CAAGCAGTTG CTGCATTATG TGCGCTTGGT GGATTTAAAG AAACCATTAA AATAGGTTCT
    GAAGTCCAGG TGTTGGGTAA AGGAATATCT GGGAGCATTG GAGTGGTTGT TTCTATTAAT
    GAACAAGAAG GTATTGCAAC AGTGAAATTT CCAGACATAG ATTTTAGAAA ATCATGCAAA
    GCATCTGACA CACTGACAGT ACCATTATTT AGGCTCTGCG TTCCACGGTC AGAGGCATTG
    CCTCTCCATA AACTGTCCAT TACTGAGAAG GTAGTGCAGG CAGTCCAGTC CATGTTACTT
    CCCCAGGAGG GCTGCCTATC TATTCATACC TCGCTTCCTG CAACAGGAGA TGCCTCAACT
    CCAGTAATGG CAGTGGTTCG ACTTCTTGCA GAGATCAGGA CAAGAGCTTG CTTGGTGATG
    GCTCAGCTGT TGGAGGACAG CTCATTTTGT GAAGAATTCA TACAGCAATG TCCAGCTGCT
    GTAGAAGTTC TAAACCTAGT GGCCCAGGAA TGCAGTCCTG GGGAGCGTCT CTCAGTGGTG
    GAAGTTCAGT GTGAAAGACT ACGAATGCTT TATCGGGACT GTGCTCGCCC CCCGCCACCA
    CCTTTGCAGG CTGAGCGCAG ACAGCCCAAG GAAATCACCT GGTCTCCTTC CAGGGTATTC
    CCTCCTGTGA GAGCCTGTAT GTTTTCATCC CATCTAACAG CAGTAACATT TCTGGCTGAC
    CCAAGTGCTG GTGGGGGACT TCCTCGCGGC ACATTTATAT ATGCAACATC CCCTGTTCCA
    GTACAGGCAC CATCTTTTTA CTGGGAAATA GAAGTAGTTT CCTATGGAGA CACAGATGAT
    GACACTGGCC CTATTGTATC ATTTGGGTTT GCTACAGAAG CAGAGAAGCG GGATGGAGCA
    TGGACAAACC CAGTCGGTAC CTGTCTTTTT CATAACAATG GTCGAGCTGT ACATTACAAT
    GGATCAAGTC TCTTACAGTG GAAGAGTGTT CGATTAGATG TGACTCTTGC TCCTGGTGAT
    GTTGCTGGAA TAGGTTGGGA GAGAACTGAA GGAACACCAC CACCCCCAGG GCAGCCTGCC
    AAGGGCAGAG TCTACTTTAC TTACTGTGGA CAGAGGCTTA CTCCGTTCTT AGAGGATGTC
    TCTGGGGGCA TGTGGCCAGT TGTGCATATT CAGAAAAAGA ACACTAAAGT CCGTGCAAAT
    TTTGGATGCA GACAGTTTGC TTTTGCCGAG GGTCAAGCTC ATCGCAATGC AGCAGATTTG
    TGCATTGATT TGGCTGAAGA GATAAGTGCA AACTTTGAAG CATTGCCCTT TGCTATGGCA
    TCTGACAGTG ATAATGATGC TGGGACAAGC ATAGCATCTG ATCCTGGAAC TCATGGACCT
    CCTTGCCGTA TTGCTGCAGT TTCTACTGTG CAACAGCAAT ATGACAGTGA TGCCTCATGC
    CGTTACAAGG TAGAGCTGAG TTATCAAGAC TTTGTGACCT CTGGACCTGA TCCACACCCA
    CCTCCCATTG CAGATGATGA AAGTGATGAT GATGACGATG ATGATATTCC TCGAGAGGAT
    CATTATGCTC TTTTGGTAAA AGCATGGGAG ACCAAAGTTT TTCCAACCAT TCGAAGACGT
    TTCCGTAACG AGGCAGAGAG AAAGTCTGGC CTGGACCAGA TTAAGGGAGC TCTTCAGCTA
    GGAATGGTGG ATATAGCGAG GCAAACTGTA GAGTTCCTCT ATGAAGAAAA TGGTGGCATT
    CCAAGAGATC TCTATCTTCC TACTATTGAG GATATCAAGG ATGAGGCAAA TAAATTCACC
    ATTGATAAAG TTCGCAAAGG ACTCACAGTG GTCATTCGAT CCCCAGATAG CAACAATGTA
    GCTGGGACAA CTGTGGGAAC AGCTTTACCC AAGTTTGCCA TCCGTGGTAT GCTGAAGACA
    TTCTCTCTAC ATGGGGTGGT TCTGGATGTG GATTCAGTTA ATGAGCTGGT TCAGGTAGAG
    ACTTACCTCC GGAGTGAAGG GGTATTAGTG AGATACTGGT ATCCCATAGA TATGCTAGAA
    AGACCTCCTG CGGGATACAG GAGAATGGCA ACCAATGGAC TGGTGACTTT AGATAGCACT
    AACATCCAGA TTCATAGAGA GCTGTTGCGG TGTGAGTCTG CACTTGCCAG GCTTTACTGT
    CGTGTGGCTT TGCTTAACAT CTTTGCTCCT AAGTCTCCGC ACACTTTCAC TCGCCTGTTC
    CATATTCCTG CTATCCGAGA CATTACATTG GAGCATTTAC AGCTTCTGTC AAATCAGCTT
    CTAATGCCAC CCCTTCCAGA CGGAACCATT ACCTCCAGCT CTATCCTTCT TGCCCAGTCC
    TTGCAACATT GCATCTACTC CCAGAACTGC TCAACAACAG ATTTGTTCTA CCAGGGCAAT
    GCACAGCCAA TAAGAGAGTG GCTTGGTGTG GCCATAACCA GGGCCCTGCA TCAAGGAGAG
    GACAGTCTCT TGGATCTGAC AAAACAAATC TGTTCCTTCT TGCAAAATGC GCCGGAACAG
    TTTCCATCTG AAGAATTTCC CATCTCTGAA TCCAAGCTCA GTATGGATGT TAATTTTCCT
    GGGGCAGCTT TTGTTATTGT TTCTTGTATA GAGAGTCCGT CTGGATTTAG AAAAGACTCC
    TCCTTGTACA AGGCTCCCTG GTCTAGAGTC ATGGTATATG GCCTTGGACA TAAAGTAAAG
    AGGAATGGAC AGCTGAACCT GATAGAATCA GTTTGTTATC CACGTGATGC CTCCCCAAGT
    AACACAGGCC TCACTCCCCC ACCAACCACA AACCAGTACC CAACAGCGAT TATTCCCACT
    GACAAGGTCC ACGTCAAACT TGGTGTATCA CCTACTCCTG GTGCTGTGCT GGTCATCCAT
    TCCCTGCCTC TAGAGTTTCC GTTGGCAATG GCATTCACAG AACAGCTGTT AACCTGGAAG
    TTGGAGGACA CAGAAGAGAA GTCAGAAGAT GAGCTTGATA CTGTTCCATC CTCTGCTCTG
    TTGCAAGTGG TAGAACTTTT AGGTACTTTG ATTTGGACAA CAGACCTTGC TGCAGTAATT
    AAGGAGCTTC TGTTCCATCT GTTGGCTGAA CTATTAAGGA AAGTTCACCA TCTAGAGCAG
    AAGAAGAACC CAAGTGGATT ATCTTCCTCG ATAGCCCTGC AGTTGAATCC ATGTCTAGCA
    ATGCTTATGG CTTTACAGAC AGAACTGCGC AAGCTGTATG ATGAAGAAAC TCAAAACTGG
    GCATCTGGGA ATGCATGTGG GGGGTCTGTA CCTGGAGCCT CTGAGCAGGG AAGATTTTCA
    ACTTACTTTC ATGCCCTCAT GGAAGTGTGC CTGGCAGTGG CAGAAGTGAC GCTCCCAATC
    AACATGAGCT CAGCAATCAG TGTGATGGCA TCTACAAATG CCACTAATCT GAGTGACTCT
    TCTTCTTCCT CTTCCTCTTC CCCTGGACAG ACTCCTCAAA GTCCAAGTCT GTTATCAAAG
    AGAAAGAAAG TTAAGATCAA ACGTGAGAAG ACTGCATCTG GAAAACGTAC ACCTTCACGT
    GGTGCAGAGA GTGATGCTGC TTTGCTTAGC ATTGGAGGAA GCAAACCTGA GGACATGTTG
    TGGTTCCATA GGGCATTGAC TTTATTGATG ATCCTAAGGC ATCTAACTAA CAAGGATCCA
    CAGGGGATGG GTGTCACAAA CGATGCTATT GCTGATGCTA GCCAGGCTTT AGTTGGTCCC
    ACAGCTCACA GTCGTCTCCT TGTGATTTCT GGCATCCCTA CACACCTAGA AGAATCAGTG
    GTCAGAAGTG CAATTAGAAA GGCATGTAAT GCTCATGGTG GAGTCTTTAA AGATGAAATC
    TACATTCCAT TGCAGGAAAC TGAAACCCCT AAACCCAAAG ACAGAATAGA AGGGGCAGAA
    TGCAAGACAG AAATAGAAAA ACCCTCTACG CAGCATACAA CCGACAATAT GGATATCAGC
    AGCTCGAGCA GTGTAACTCC TGCAGCAAGT GTCACGGCAT CAGCTTCTAC AAGCCAAGCT
    TCATTGTGCA GCTCCCAAGG GGGGGTATCA CGGACCACAA GTGAAACATC TATGGAGCAG
    TTTGCCCCAG AACATGCTGT GCCATCAGGG CTTCTGGAAC CAACTATTAT CTCCAGCCAG
    GAGAGTCTGG ATCTTTCTAT GTGCAGCACA GGAAGCCTCG GCAGTCTGGG AAGTCTTGTT
    GAACAGCTTG ATAATGTTGA AAACTCTTTG TCTGACGTGG GCTCCCTATA CACAGCTACA
    TCACTAGATA GCCATATGGC AGAGTCGCGG CCAATCAAAG GATTTGCAGT TGTAGAGGTG
    CGATCACGAG CTAAAATAGA GAAAATCCGT GCCACTTTAT TTAACAGTAG TGACCTCATT
    GGGTTATCTG GTTTGGATGG TGAAGATGAG CTGATGGAAA TGTCTACTGA GGAGATCTTG
    ACTGTTTCAA CAGTGAATCA GAGCTTGTTT GATACCCAAG GAATACCCGG TCTGGCTGAT
    TATTTCATGG ATAAATCAAT GAAAGGTGAT AAATTAGTCC CGGAAGCTCG TGATGTTCTG
    ACTGAGATTT TCAAGAGCTG CATTCACGCC GAGCAGATGC TGAGTCTGAC TCCAGCCAAG
    TTTATCAAAG TGTCTGATAT CTACCTTAGC AAGGAGCAAA TCAATTCACA GACCCCAGGG
    AACTTACTGC ATACCTTTTT CACCAATGTT CGACCACCTA AAAAAGTCCT GGAAGATCAA
    CTAACGCAGA TCTTAAGAAA ATATGGAATT CCCAAGCCTA AATTTGACAA AAGCAAATAC
    AGCAAAACTG GGAAGGAGCA ACATCCTGTG AAAGTTGTAA GCACCAAAAG ACCTGTGGGC
    AAACCACCGA CCAAAGACAA GGCTGTATTC AACAGCGTCA GGACTGCTTT AAGTGAGAAA
    AAACCAACTG TGAAGCCAAA ATCTCCGGAG AAGGGCAGGC CAGATGAGAA GGACCCAGAC
    AAATCTCCAA CAAAGAAGCA AGAAAGGTTT CACTTGTGTC CCTATCTAGT TCCCGAGGAG
    AGATATCTGA CGTTGGATGG ATTTCACCGC TTTGTAGTAG ATCGGGCTAA GCAAGACATT
    CGAAGCATCT GGAGAGCTGT CTTGGCGTGT GGATATGATC TTCATTTTGA AAGGTGTGCC
    TGCATTGATA CAAGGCATGC TCAGAAATCT AGCAGAAAAT GGACCTTGGA AATGGATGTT
    GCTCTTGTTC AGTATATCAA CCGCCTTTGC CGTCACCTTG CCATAACGCC AGCCCGACTA
    CACCCTCATG AGGTTTATCT GGATCTTGCA GATACGGCTG ACCCAAGAGT CTCCTGCCTG
    CTGAATGTAC CAATAGAAAG CCTGCGCCTC CGATTTGCTC TTCTTCAGTC CCTCAACACT
    ACTTTAGAGA CCTTTTTCCT GCCCTTAGTG GAGCTACGGC AAACAGACGT ATACACACAC
    AGCATCGCAT CCCTACTCCA GCTAGCTAAA GGGTTAATCT TTTACGATAC AAAAGTGAAT
    GTGATGAACC GGGTATTGAA CGCAACAGTG CAGAGGACAG CAGATCATGC AGCCCCTGAG
    ATCACGCTGG ACCCACTAGA AATTGTAGGA GGGGAAATTC GTACCTCAGA AAACTCCTAC
    TTCTGCCAGG CAGCCCGACA GTTGGCATGC ATCCCTTCCT CACAACTGTG TGTCAAACTG
    GCAAGTGGAG GAGACCCCAC GTATGCATTT AACATCCGCT TTACTGGAGA GGAGGTGCAT
    GGTACAAGTG GCTCATTCCG TCATTTCCTC TGGCAAGTGT GTAAAGAACT ACAGAGCTCC
    TCGCTGTCTC TTCTACTGCT CTGCCCCAGC TCTGCAGTTA ATAAGAATAA GGGAAAATAT
    CTTCTAACTC CCAGTCCCAT AACATATGCA GAAGAGCAGT TGCTCCATTT CTTTGGCCAA
    CTACTGGGAA TAGCCATCAG AGCCGATGTT CCACTCCCTC TGGATCTACT CCCCTGCTTC
    TGGAAGATGC TTGTAGGAGA ACCTTTGGAC CCAGAAACTG ATCTTTATGA AGCAGATATT
    CTCACCCACA ACTACATCAA GAAGTTTGAA AATGTAAATG ATGAGTCAGA GCTGGAAGCT
    CTTTGTGCTG AAATTGCCTC TCAGCATCTT CCTACCGAAA GTCCAGATTC TCCTCATAAA
    CCAGCCTGCA AATTTACATA CTTGACAATG ACTGGTGAAG AAGTGGAGCT GTGCCCTAAG
    GGAAGACACA TTTCTGTAGT CTGGGAAAAC AAAGATATAT ATGCAATGGG AATCCGGAAC
    CTTCGTATAC GTGAGCTGAA GAACCAGGAG TGTATGAGCG CAGTAAGGGC TGGGCTGGGC
    TCCATTATAC CTCTGCAGCT CCTCACCACT CTAAATGCTC TAGAGATGGA ACTAAGGACA
    TGTGGCCTAC CATATATTAA TTTGGAATTC TTAAAGGCTC ACACCATGTA TCAAGTGGGT
    CTAATGGAGA CAGATCAACA CATTGAGTAT TTTTGGTCTG CCTTAGAAAT GTTCACTCAG
    GAAGAACTTT GCAAATTCAT AAAATTCGCC TGCAATCAGG AACGCATTCC CTTTACATGT
    CCATGCAAAG ATGGTGGGCC AGACACGGCT CATGTTCCAC CTTATCCTAT GAAAATAGCA
    CCACCCGATG GAGCAACAGG CTCCCCAGAT TCCAGGTACA TCCGAGTGGA GACATGTATG
    TTCATGATCA AGCTTCCGCA GTACTCGTCT CTAGAGATGA TGCTGGAGAA ACTGAGATAT
    GCCATTCACT ACAGGGAAGA CCCACTCAGC GGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa49026
    Clone ID Related Accession (Same CDS sequence) XM_012968799.3
    Accession Version XM_012968799.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13218bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product probable E3 ubiquitin-protein ligase HECTD4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012968799.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGGGGTCGG CGGCTGCCGG GGACTCGGCT CAGTGGCTGT CGGTGAAAGA AGAGACTATC 
    TTTTTACATG ATGGGCTGAT CCGGGTCACA GACCTGGCGG AGTTGCCCAG CGAAATCGGG
    GTCAGTGAGG CCGGGGACGC AGAACTGGAG ATATTGACTT TTGAGACTAA GAACCCTATT
    GAGCTAGCAG AGCATTTACG TGCAGTGTGT GGGAACCAGA GCAATGCCTA TGCACGATTG
    CTGGAATACC GCTTGAATGC GTTACGTGGG TTATGGAATG CCCAACGCCA GCTGGCGCTT
    GAGGATCAAC ATGAAAGAGA TGGGGCTGAT GACGAAACCT TGGCCCTGCT TAAACGGCAG
    GGTTTGTTGC AACAGCCGGA ACAGGCTCCA TTTACATCTC GAATGGGGCT TTTACTGGTG
    TTCCCCCTTA TCCAGTCCCA AAGTAAAACA GACCCTTCAC TTTGTAACTT GACAGCTGAG
    GTGCTTTTAA ATTGCCTTCG GGACTGCCAA CCCCTGAGCT TGACTAAAGA ACCAGCTGAC
    TGCCTGAATG GAATAGAGTC CTTACTGTGT TCTTGGTTAG AAGACACTCC AAATGTGGGC
    CAACAAGTCT CCTTTAAGCA AAAGGAAAAT TCTGCTGCTG CTTTGGTTGC CTTAGCTTGT
    GCTAGGGGAT CATTAAAAAC ATTTATTCAC ACTGTACACT TGCTGCAAAA ACAGACTGAC
    CTAGGATCCC TTCCTGTGGC AGATGTATTG TATAGGTTGC TGCTTTTAGA GGGAGGTCCA
    GGTTCTCCCT CTTGTTTACT GGGAAGCAAA CACATTGTAT CCTGGGGTTT TGAGGATATG
    CTTCCAGCTC CTGATGGGAT CAGTGGTTCA AACAATGAGA ACAAAGATGC AGATTTGGGT
    CGCTGTGTAG CATGCGATGG GCTTTATCTT TACACAACTA ACTCTGTAGG TAGAGGATTG
    AGCAAAGTAG GGTCTGGGCT GCATGGCACT TTACGAGGTT TTGTTTATTC CCGAAACGAG
    GAGCTAGAGG CTGGCTGGAT TGCTTATGGA AGTGGGAAGA TACTTCACAG GCCTGCCTCC
    TTTGATAACA AACCTCAATA TCTTTTCCAA TTAATTGACC AATACACACT TCAGGTCTCT
    TATATAGTCA GCATGCCAGC CAATCATTTC CCGGTTGGCA GCAATATGAC TACGGTGCAC
    CTCTCCTCGG ATGGCACGTA TTTTTACTGG ATTTGGTCTC CAGCTAGTTT AAATGAGAAA
    ACCCCCAAAG GACACTCTGT CTTCATGGAT ATATTTGAGT TAAATGTGCA AAATGGAGTA
    TGCATTGCAA GCCCTCTTCA TGAGCGCATT ATATTAATGA GGAAAGAGGG AGAATCTGCT
    AAAAGTATTA ATGAAATGCT TTTGAGCAGA TTATCACGAT ACAGAGCTTC TCCTTCAGCA
    ACTCTAGCAG CACTTACAGG ATCTACAATC TCTAACACTC TAAAAGAGGA CCAGACTGCT
    GCAAATACGA GCTGCGGGCT TCCTTTGAAA ATGTTAAGAA AGACTCCAAT GTATACTTGT
    GGCACATACT TAGTACTACT CGTGCCACCT CCGGGTGGGT CAGGAAGTTC AGCAACAAGA
    TCTTTGTTTG GTGGAACCAG CGGCCTCTCC TCTTTAAAAA TTCTGGCTTC CAGCTTGGTA
    TTTAACATTT CTGATGGGCA GTTCACCAAC AGAGCAGATC TTATTGATGC ATCTGGAAGT
    TCTCTTGGAA GGGGTGCTTT GGTGACAGGC CTAGGTGCCT GCTATGATAC AATGAATAAC
    ATGATCTGGA CGTGCTCCAA TGATTACATC GATCAGTGGT GTAATCCTGG AAACCAGGCT
    TTCCATGTGG TGTGTCAGAG GCTTGGTGTC ACTCACATCA TTGCTGATCC CAAAGGAGAG
    GCAGTATCAA CAAATGAAGT TATAAACCAG TTGCTTCACC ATGTGGGTGC TATGTGTATT
    CACCAACTCA ATCTCCTGGC TAATAGCAAC AGTTCTCCAA TCAGTACCTT TCTGGGCAAA
    CAACATCCTA TAGAGCCACA TCATCTTAAT AGTATATGTG ACATCATGGA GAAAGCCATG
    GTCAATGGTG ATAAATGCAT TATTCGCTGC ATCTTGGTTG TTTTCCAGGT GGTATTTAAA
    TTCTTTCTAA ACCCACAAAC TGAGAGGAAT CGAGAAACCA TAAGGAGGGC TGGTTTGCTC
    CTTTGGCAGT TGCTCATGGC GCCTAAGGAC CAAATTTGTC CTGAAATACA GAGAGAGGTC
    TGCTTGGCTA TTAGCTTTGG TTTAAACATT TTGAATCCAG GAGAGCTAGA AATTAATAAC
    TTGCTCAAGC TGATTTTAAC TGAAGGGGAC AGAAATTGTG GCCTCTCCCA ACTCCGTGAT
    GTCATTTTGA CAAACCTCAC TGAACAGCTT CAGAATAACA GGTTTGGAAG TGAAGATGAT
    GAACATTACA GGCTTAATGA TGAACTATTG CACTATATTT TGAAGATTGT TGTTCGTGAA
    TCCTGTGTTC TGATCACCAA GTGCCAAACA GTGTCTAAAG AAGAATTCCA AAGACTCTTG
    TCAACAGTGC CTGTGGCTTC TCCCTGTCTA TGCTATTTAA TGGCTGTACA GAATCATCTC
    CTGAGTAACA CTGTCTTAAT TAAACCTGAT GACAGCGATG ACAGTGACAA CTCCCTGCAG
    GGGGAGACAT TGAAGGTACA GGAACTCCAG GCCAGCATCT TGGCTTTGGC AACCCAGATA
    CTTTCTGGTT GTGATGAAGT TCTGGATATT CTGCAGCGAG TGACCACTGC TCTTATCAAC
    AGCGACCTGT TAGACAGAGA TTTAAGACTG AAAGGCCTTG AACAGATCAC AAAGTCCACC
    CTGCTTGGCC ATCTCCTCCC AGTACTTCTC ACATCCCTTA TGCATCCAAA CTTACAGACA
    TTAACCATGG CAGATGCGCT TATGCCTCAG CTGGTGCAGC TTGTTCTTTA CACTAGCCAG
    ACTGCACTGC TGCTTAAAAC CCAGTGTCCA GTATTGGCAG ATACAGGGGA CACCACCAGT
    GGAATTCCAG AGCAAAAAAA CAAACTGTTA ACTGATGACA GGATGCTGGA AGAAAAGGAG
    GAGCCTGGTT TCCTCACTGG GCTAAAGATT CCTGCGCCAT GGGCTGCAGG AAAGACTGTG
    GAAACATCTC ATCCAGTCAG AGACAATTAC AAGTTTAAAG AGACTGTGCA CATTCCTGGA
    GCTCGTTGTT TGTACCTCCG GTTTGACAGC AGGTGCTCTT CACAGTACGA TTATGATAAG
    CTGGTGATAT ATGCGGGACC CAACACAAAC AGCAGAAAAG TTGCCGAATA TGGTGGAAAT
    ACCCTGGGGT ATGGCAGTAG AAGTGTTTTA GGTACAGGCT GGCCAAAAGA TTTGGTTAAG
    GTGGAAGGTG ATACTGTGAC ATTCTCCTTC GAAATGAGAA GTGGGCGTGA GCACAACACT
    CCTGATAAAG CAATGTGGGG TTTTGCGTGC ACTGTCAGAG CTCAGGAGTC CTCAGAAGAT
    GTGTCAGGGG GCTTGCCATT TCTAGTAGAC CTGGCTTTAG GTCTGTCTGT CTTGGCTTGT
    TCCATGCTGC GTATATTGTA CAATGGTCCA GAAATCACAA AAGAAGAGTT GACTTGTCAC
    GATCTGTTGA GGTCAAAGCT TTTGCAGAGG TGTCAGTGGC AGGTGGAGGC TAATGGTGCT
    TTATCCCCGG CACTCACCCC AAGCCCTTCG CCGCTGCCCC TTACTATAGA AGAAGACCAA
    GAATTTACGT ATCCATCTGA CATCTTGTTG CCACCAGTTG GGAACTATTT TGAACTGCCA
    AGAATCAGAC TGCCTCCTGG TATTATGATC AAGCTCAGGG AAATATCTGG ACGCGCTCGA
    CCACAGTTTA GACCAAGCAT AAAAGAGGTT ATCCAGCCTG ATGTGATGGA AGAAATGGTT
    GTATCTTGCG TCATTAAACA CTTGAACCTG GTCGATGCAT TGCAGTCTTT AATAAACTTC
    CAGTATCGAG AAGAACATCT AGAAGAATAT GATTTATTAT GTAAAATAAT GGGGGAAACT
    TTTAAGAAGC TCAATGCAAT GGAAAGACAA TTGCAGAGTG TTGCAGAACT GGAACAAAAG
    TGGCAAAGTG AAGTGGAAGA TGCTCAGAAT GGAAAGCTGG AGAACAACGC TCCCTTTTTT
    TACGACTATC ATTTTAATGA GAGCAAGTTA AAGGAACTTG AACTTTTATG TTCTATGAAA
    GAGGTCCCTT CTGATGGAAG TGATCCAGAA AACATGGTTT TCTCTCTGCG AGAGAAGTTT
    TTTCAAGAGG TGAACTCCCT GATCCAAAAA CCTTCCCACC CAATGGCTAA GACCAAAACA
    TTGGTTAAGA GTCTGATGAA TAGAGCAGAG CTCCTGTTAC ATGTGACTAT TGCAGCACAT
    TCTGGTATCA CGAGAAGCAT ATCTGGCACT CCAACTGAAC CTCAAGCTGG CAAGTCTATC
    TCAGAAGCAA AAGTTATATC TCAAGCCATG CGCCAGCCTG TTTTCCTCCG CAGCATGTCT
    GCCCCTTCTG ACCTGGAAAT GATTGGAAGT AGTGACTTAG AGTTCAGCAG GTCTAATCCA
    AGGAGAAGGA ACATCACTAG CCACAGGAGC AGCTCCTTCA CTCTGCTTCA GTCTTTAGCT
    ATTGAAGACT GCAAGGATAA ACCAACATAC AGTGTCCTTT TAGGGCAACT GTTTGCCTTC
    ATTGGGACCA ATCCCGACCA AGCAGTATCC AGTAGCAGCT TTCTACTTGC AGCCCAAACC
    AGATGGCGTC GTGGTAATAC TCGCAAACAA GCCCTTGTAC ACATGAGAGA ATTACTGACT
    GCTGCTGTCC GTGTTGGGGG TGTGACACAT TTGGTTGGAC CAGTAACAAT GGTGCTACAG
    GGAGGACCAA GAATTGAAGA GCTCACCTGT GGAGGAATGG TGGAGCTTGT TCAAGAGGCA
    TTTGGAGAGA CCATGACCTC AGTGGTGTCA CTTTGTGCAC GGTATCCCAT TGCCTGTGCA
    AATAGCATTG GGCTTTTGTG TACTATACCT TACACAAGGA GTGAAGAAAA ATGTTTAGTT
    CGCAGTGGGC TTGTTCAACT GATGGACCGA CTCTGCAGCT TAAGCAACCA GAGAGAGTCC
    AGCTCAAATG AAAAGCAAAC CAAGAAACAG AAAGTAGCAA CTATGGCATG GGCAGCCTTC
    CAAGTACTGG CCAATAGATG TGTAGAATGG GAGAAAGAAG AAGGTGGTTC TACTGAGGCG
    GTACACTCTG GTTTGGCTAG ACAAGTCTCC ACCCTCCTCA CTAACCATCT AGCAAGGGCT
    ACAGAGTGCT GTGGAAACCA AGCTGCAGGG AATGATGCCC TACAGGACGT TCTGAGCCTT
    CTCAATGATC TCTCTAGGAG TCAGATAGGG AAGGCAATTC TGAGCCAGCC TGCTTGTGTG
    TCCAAACTTC TTTCGTTGCT GCTGGACCAG CGTCCTTCTC CAAAGCTTGT GCTCATAATT
    CTGCAGCTGT GCCGCGCTGC ACTTCCTCTC ATGAGTGTTG AAGACTGTGG AAATGTAGAA
    CTTCCTCCAT GGAGTTACTC TTTACCAGCT CTGGACAGTG AACAGGGGGA GGATCCAAGT
    GACCCAGCCT CCAAAATAGC ATCTTTGTTG CTAGCAAAGT TGGCTGATTA TGTTGTTCCA
    GGATGTCAGA CAGTTCTTTC TCCTACTGCT TCTGAACCAG ACAGCTCATT GTCCAAAAAC
    AGCCCAAAAA ATTCCCAGAA AGCAGATAAG GATGCAGAAG AAGAGAGTGA AGCAGTAGAT
    GGCAAGCTCT CCATTTTTAT TCATAAGCGA GAAGACCAGT CATCCCATGA GGTTCTTCAG
    CCTTTGCTTA GTAGTTCAGA GGGAAGGCCT TTTCGTCTTG GTACTGGAGC GAATATGGAA
    AAAGTAGTAA AGATGGATCG TGACATGACT AAGAGTGGAT GTTGTGAAGT AATCACAGAG
    GAGGCTGCAG CTGCTCTCCG CAAAGCCACT AAATGGGCCC AGTCGGGTTT AATTGTTAGC
    ATTGGGCCAC CTGTGGAGAC AGTTAGCCCT GATTCAGCGA GTGGACTTTC CACTGGTGAC
    AAAAAGAAAA CAGCCCAGAC CTCGATCTGT AGGGAAAGGA ATTCTGAACT TGCCAGGACT
    GATCCTGTAC GCCCTTTCAT TAGTGGCCAT GTAGCAAACA GCATGGCTGC AGAAGTAATT
    GCTCTGCTTC ACAGCCTGCT GATGGCACCA GAGTCAAATG CTGCCCAAAT CTGGACAACA
    ACAGCAGAGA AGGTTCTTTC TCGTGCCCTG ATGTATATCC CACAGCTTGG CAAATATGCA
    GAAAGCATCC TAGAGAACGG CAGCGGTAAT GGCAGGAAGC TAGCAAAGCT ACAAGGCATT
    GCAAGGCAAG CAGTTGCTGC ATTATGTGCG CTTGGTGGAT TTAAAGAAAC CATTAAAATA
    GGTTCTGAAG TCCAGGTGTT GGGTAAAGGA ATATCTGGGA GCATTGGAGT GGTTGTTTCT
    ATTAATGAAC AAGAAGGTAT TGCAACAGTG AAATTTCCAG ACATAGATTT TAGAAAATCA
    TGCAAAGCAT CTGACACACT GACAGTACCA TTATTTAGGC TCTGCGTTCC ACGGTCAGAG
    GCATTGCCTC TCCATAAACT GTCCATTACT GAGAAGGTAG TGCAGGCAGT CCAGTCCATG
    TTACTTCCCC AGGAGGGCTG CCTATCTATT CATACCTCGC TTCCTGCAAC AGGAGATGCC
    TCAACTCCAG TAATGGCAGT GGTTCGACTT CTTGCAGAGA TCAGGACAAG AGCTTGCTTG
    GTGATGGCTC AGCTGTTGGA GGACAGCTCA TTTTGTGAAG AATTCATACA GCAATGTCCA
    GCTGCTGTAG AAGTTCTAAA CCTAGTGGCC CAGGAATGCA GTCCTGGGGA GCGTCTCTCA
    GTGGTGGAAG TTCAGTGTGA AAGACTACGA ATGCTTTATC GGGACTGTGC TCGCCCCCCG
    CCACCACCTT TGCAGGCTGA GCGCAGACAG CCCAAGGAAA TCACCTGGTC TCCTTCCAGG
    GTATTCCCTC CTGTGAGAGC CTGTATGTTT TCATCCCATC TAACAGCAGT AACATTTCTG
    GCTGACCCAA GTGCTGGTGG GGGACTTCCT CGCGGCACAT TTATATATGC AACATCCCCT
    GTTCCAGTAC AGGCACCATC TTTTTACTGG GAAATAGAAG TAGTTTCCTA TGGAGACACA
    GATGATGACA CTGGCCCTAT TGTATCATTT GGGTTTGCTA CAGAAGCAGA GAAGCGGGAT
    GGAGCATGGA CAAACCCAGT CGGTACCTGT CTTTTTCATA ACAATGGTCG AGCTGTACAT
    TACAATGGAT CAAGTCTCTT ACAGTGGAAG AGTGTTCGAT TAGATGTGAC TCTTGCTCCT
    GGTGATGTTG CTGGAATAGG TTGGGAGAGA ACTGAAGGAA CACCACCACC CCCAGGGCAG
    CCTGCCAAGG GCAGAGTCTA CTTTACTTAC TGTGGACAGA GGCTTACTCC GTTCTTAGAG
    GATGTCTCTG GGGGCATGTG GCCAGTTGTG CATATTCAGA AAAAGAACAC TAAAGTCCGT
    GCAAATTTTG GATGCAGACA GTTTGCTTTT GCCGAGGGTC AAGCTCATCG CAATGCAGCA
    GATTTGTGCA TTGATTTGGC TGAAGAGATA AGTGCAAACT TTGAAGCATT GCCCTTTGCT
    ATGGCATCTG ACAGTGATAA TGATGCTGGG ACAAGCATAG CATCTGATCC TGGAACTCAT
    GGACCTCCTT GCCGTATTGC TGCAGTTTCT ACTGTGCAAC AGCAATATGA CAGTGATGCC
    TCATGCCGTT ACAAGGTAGA GCTGAGTTAT CAAGACTTTG TGACCTCTGG ACCTGATCCA
    CACCCACCTC CCATTGCAGA TGATGAAAGT GATGATGATG ACGATGATGA TATTCCTCGA
    GAGGATCATT ATGCTCTTTT GGTAAAAGCA TGGGAGACCA AAGTTTTTCC AACCATTCGA
    AGACGTTTCC GTAACGAGGC AGAGAGAAAG TCTGGCCTGG ACCAGATTAA GGGAGCTCTT
    CAGCTAGGAA TGGTGGATAT AGCGAGGCAA ACTGTAGAGT TCCTCTATGA AGAAAATGGT
    GGCATTCCAA GAGATCTCTA TCTTCCTACT ATTGAGGATA TCAAGGATGA GGCAAATAAA
    TTCACCATTG ATAAAGTTCG CAAAGGACTC ACAGTGGTCA TTCGATCCCC AGATAGCAAC
    AATGTAGCTG GGACAACTGT GGGAACAGCT TTACCCAAGT TTGCCATCCG TGGTATGCTG
    AAGACATTCT CTCTACATGG GGTGGTTCTG GATGTGGATT CAGTTAATGA GCTGGTTCAG
    GTAGAGACTT ACCTCCGGAG TGAAGGGGTA TTAGTGAGAT ACTGGTATCC CATAGATATG
    CTAGAAAGAC CTCCTGCGGG ATACAGGAGA ATGGCAACCA ATGGACTGGT GACTTTAGAT
    AGCACTAACA TCCAGATTCA TAGAGAGCTG TTGCGGTGTG AGTCTGCACT TGCCAGGCTT
    TACTGTCGTG TGGCTTTGCT TAACATCTTT GCTCCTAAGT CTCCGCACAC TTTCACTCGC
    CTGTTCCATA TTCCTGCTAT CCGAGACATT ACATTGGAGC ATTTACAGCT TCTGTCAAAT
    CAGCTTCTAA TGCCACCCCT TCCAGACGGA ACCATTACCT CCAGCTCTAT CCTTCTTGCC
    CAGTCCTTGC AACATTGCAT CTACTCCCAG AACTGCTCAA CAACAGATTT GTTCTACCAG
    GGCAATGCAC AGCCAATAAG AGAGTGGCTT GGTGTGGCCA TAACCAGGGC CCTGCATCAA
    GGAGAGGACA GTCTCTTGGA TCTGACAAAA CAAATCTGTT CCTTCTTGCA AAATGCGCCG
    GAACAGTTTC CATCTGAAGA ATTTCCCATC TCTGAATCCA AGCTCAGTAT GGATGTTAAT
    TTTCCTGGGG CAGCTTTTGT TATTGTTTCT TGTATAGAGA GTCCGTCTGG ATTTAGAAAA
    GACTCCTCCT TGTACAAGGC TCCCTGGTCT AGAGTCATGG TATATGGCCT TGGACATAAA
    GTAAAGAGGA ATGGACAGCT GAACCTGATA GAATCAGTTT GTTATCCACG TGATGCCTCC
    CCAAGTAACA CAGGCCTCAC TCCCCCACCA ACCACAAACC AGTACCCAAC AGCGATTATT
    CCCACTGACA AGGTCCACGT CAAACTTGGT GTATCACCTA CTCCTGGTGC TGTGCTGGTC
    ATCCATTCCC TGCCTCTAGA GTTTCCGTTG GCAATGGCAT TCACAGAACA GCTGTTAACC
    TGGAAGTTGG AGGACACAGA AGAGAAGTCA GAAGATGAGC TTGATACTGT TCCATCCTCT
    GCTCTGTTGC AAGTGGTAGA ACTTTTAGGT ACTTTGATTT GGACAACAGA CCTTGCTGCA
    GTAATTAAGG AGCTTCTGTT CCATCTGTTG GCTGAACTAT TAAGGAAAGT TCACCATCTA
    GAGCAGAAGA AGAACCCAAG TGGATTATCT TCCTCGATAG CCCTGCAGTT GAATCCATGT
    CTAGCAATGC TTATGGCTTT ACAGACAGAA CTGCGCAAGC TGTATGATGA AGAAACTCAA
    AACTGGGCAT CTGGGAATGC ATGTGGGGGG TCTGTACCTG GAGCCTCTGA GCAGGGAAGA
    TTTTCAACTT ACTTTCATGC CCTCATGGAA GTGTGCCTGG CAGTGGCAGA AGTGACGCTC
    CCAATCAACA TGAGCTCAGC AATCAGTGTG ATGGCATCTA CAAATGCCAC TAATCTGAGT
    GACTCTTCTT CTTCCTCTTC CTCTTCCCCT GGACAGACTC CTCAAAGTCC AAGTCTGTTA
    TCAAAGAGAA AGAAAGTTAA GATCAAACGT GAGAAGACTG CATCTGGAAA ACGTACACCT
    TCACGTGGTG CAGAGAGTGA TGCTGCTTTG CTTAGCATTG GAGGAAGCAA ACCTGAGGAC
    ATGTTGTGGT TCCATAGGGC ATTGACTTTA TTGATGATCC TAAGGCATCT AACTAACAAG
    GATCCACAGG GGATGGGTGT CACAAACGAT GCTATTGCTG ATGCTAGCCA GGCTTTAGTT
    GGTCCCACAG CTCACAGTCG TCTCCTTGTG ATTTCTGGCA TCCCTACACA CCTAGAAGAA
    TCAGTGGTCA GAAGTGCAAT TAGAAAGGCA TGTAATGCTC ATGGTGGAGT CTTTAAAGAT
    GAAATCTACA TTCCATTGCA GGAAACTGAA ACCCCTAAAC CCAAAGACAG AATAGAAGGG
    GCAGAATGCA AGACAGAAAT AGAAAAACCC TCTACGCAGC ATACAACCGA CAATATGGAT
    ATCAGCAGCT CGAGCAGTGT AACTCCTGCA GCAAGTGTCA CGGCATCAGC TTCTACAAGC
    CAAGCTTCAT TGTGCAGCTC CCAAGGGGGG GTATCACGGA CCACAAGTGA AACATCTATG
    GAGCAGTTTG CCCCAGAACA TGCTGTGCCA TCAGGGCTTC TGGAACCAAC TATTATCTCC
    AGCCAGGAGA GTCTGGATCT TTCTATGTGC AGCACAGGAA GCCTCGGCAG TCTGGGAAGT
    CTTGTTGAAC AGCTTGATAA TGTTGAAAAC TCTTTGTCTG ACGTGGGCTC CCTATACACA
    GCTACATCAC TAGATAGCCA TATGGCAGAG TCGCGGCCAA TCAAAGGATT TGCAGTTGTA
    GAGGTGCGAT CACGAGCTAA AATAGAGAAA ATCCGTGCCA CTTTATTTAA CAGTAGTGAC
    CTCATTGGGT TATCTGGTTT GGATGGTGAA GATGAGCTGA TGGAAATGTC TACTGAGGAG
    ATCTTGACTG TTTCAACAGT GAATCAGAGC TTGTTTGATA CCCAAGGAAT ACCCGGTCTG
    GCTGATTATT TCATGGATAA ATCAATGAAA GGTGATAAAT TAGTCCCGGA AGCTCGTGAT
    GTTCTGACTG AGATTTTCAA GAGCTGCATT CACGCCGAGC AGATGCTGAG TCTGACTCCA
    GCCAAGTTTA TCAAAGTGTC TGATATCTAC CTTAGCAAGG AGCAAATCAA TTCACAGACC
    CCAGGGAACT TACTGCATAC CTTTTTCACC AATGTTCGAC CACCTAAAAA AGTCCTGGAA
    GATCAACTAA CGCAGATCTT AAGAAAATAT GGAATTCCCA AGCCTAAATT TGACAAAAGC
    AAATACAGCA AAACTGGGAA GGAGCAACAT CCTGTGAAAG TTGTAAGCAC CAAAAGACCT
    GTGGGCAAAC CACCGACCAA AGACAAGGCT GTATTCAACA GCGTCAGGAC TGCTTTAAGT
    GAGAAAAAAC CAACTGTGAA GCCAAAATCT CCGGAGAAGG GCAGGCCAGA TGAGAAGGAC
    CCAGACAAAT CTCCAACAAA GAAGCAAGAA ATTCCCGAGG AGAGATATCT GACGTTGGAT
    GGATTTCACC GCTTTGTAGT AGATCGGGCT AAGCAAGACA TTCGAAGCAT CTGGAGAGCT
    GTCTTGGCGT GTGGATATGA TCTTCATTTT GAAAGGTGTG CCTGCATTGA TACAAGGCAT
    GCTCAGAAAT CTAGCAGAAA ATGGACCTTG GAAATGGATG TTGCTCTTGT TCAGTATATC
    AACCGCCTTT GCCGTCACCT TGCCATAACG CCAGCCCGAC TACACCCTCA TGAGGTTTAT
    CTGGATCTTG CAGATACGGC TGACCCAAGA GTCTCCTGCC TGCTGAATGT ACCAATAGAA
    AGCCTGCGCC TCCGATTTGC TCTTCTTCAG TCCCTCAACA CTACTTTAGA GACCTTTTTC
    CTGCCCTTAG TGGAGCTACG GCAAACAGAC GTATACACAC ACAGCATCGC ATCCCTACTC
    CAGCTAGCTA AAGGGTTAAT CTTTTACGAT ACAAAAGTGA ATGTGATGAA CCGGGTATTG
    AACGCAACAG TGCAGAGGAC AGCAGATCAT GCAGCCCCTG AGATCACGCT GGACCCACTA
    GAAATTGTAG GAGGGGAAAT TCGTACCTCA GAAAACTCCT ACTTCTGCCA GGCAGCCCGA
    CAGTTGGCAT GCATCCCTTC CTCACAACTG TGTGTCAAAC TGGCAAGTGG AGGAGACCCC
    ACGTATGCAT TTAACATCCG CTTTACTGGA GAGGAGGTGC ATGGTACAAG TGGCTCATTC
    CGTCATTTCC TCTGGCAAGT GTGTAAAGAA CTACAGAGCT CCTCGCTGTC TCTTCTACTG
    CTCTGCCCCA GCTCTGCAGT TAATAAGAAT AAGGGAAAAT ATCTTCTAAC TCCCAGTCCC
    ATAACATATG CAGAAGAGCA GTTGCTCCAT TTCTTTGGCC AACTACTGGG AATAGCCATC
    AGAGCCGATG TTCCACTCCC TCTGGATCTA CTCCCCTGCT TCTGGAAGAT GCTTGTAGGA
    GAACCTTTGG ACCCAGAAAC TGATCTTTAT GAAGCAGATA TTCTCACCCA CAACTACATC
    AAGAAGTTTG AAAATGTAAA TGATGAGTCA GAGCTGGAAG CTCTTTGTGC TGAAATTGCC
    TCTCAGCATC TTCCTACCGA AAGTCCAGAT TCTCCTCATA AACCAGCCTG CAAATTTACA
    TACTTGACAA TGACTGGTGA AGAAGTGGAG CTGTGCCCTA AGGGAAGACA CATTTCTGTA
    GTCTGGGAAA ACAAAGATAT ATATGCAATG GGAATCCGGA ACCTTCGTAT ACGTGAGCTG
    AAGAACCAGG AGTGTATGAG CGCAGTAAGG GCTGGGCTGG GCTCCATTAT ACCTCTGCAG
    CTCCTCACCA CTCTAAATGC TCTAGAGATG GAACTAAGGA CATGTGGCCT ACCATATATT
    AATTTGGAAT TCTTAAAGGC TCACACCATG TATCAAGTGG GTCTAATGGA GACAGATCAA
    CACATTGAGT ATTTTTGGTC TGCCTTAGAA ATGTTCACTC AGGAAGAACT TTGCAAATTC
    ATAAAATTCG CCTGCAATCA GGAACGCATT CCCTTTACAT GTCCATGCAA AGATGGTGGG
    CCAGACACGG CTCATGTTCC ACCTTATCCT ATGAAAATAG CACCACCCGA TGGAGCAACA
    GGCTCCCCAG ATTCCAGGTA CATCCGAGTG GAGACATGTA TGTTCATGAT CAAGCTTCCG
    CAGTACTCGT CTCTAGAGAT GATGCTGGAG AAACTGAGAT ATGCCATTCA CTACAGGGAA
    GACCCACTCA GCGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012824253.1
    CDS320..13537
    Translation

    Target ORF information:

    RefSeq Version XM_012968799.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 4 (hectd4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012968799.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGGGGTCGG CGGCTGCCGG GGACTCGGCT CAGTGGCTGT CGGTGAAAGA AGAGACTATC 
    TTTTTACATG ATGGGCTGAT CCGGGTCACA GACCTGGCGG AGTTGCCCAG CGAAATCGGG
    GTCAGTGAGG CCGGGGACGC AGAACTGGAG ATATTGACTT TTGAGACTAA GAACCCTATT
    GAGCTAGCAG AGCATTTACG TGCAGTGTGT GGGAACCAGA GCAATGCCTA TGCACGATTG
    CTGGAATACC GCTTGAATGC GTTACGTGGG TTATGGAATG CCCAACGCCA GCTGGCGCTT
    GAGGATCAAC ATGAAAGAGA TGGGGCTGAT GACGAAACCT TGGCCCTGCT TAAACGGCAG
    GGTTTGTTGC AACAGCCGGA ACAGGCTCCA TTTACATCTC GAATGGGGCT TTTACTGGTG
    TTCCCCCTTA TCCAGTCCCA AAGTAAAACA GACCCTTCAC TTTGTAACTT GACAGCTGAG
    GTGCTTTTAA ATTGCCTTCG GGACTGCCAA CCCCTGAGCT TGACTAAAGA ACCAGCTGAC
    TGCCTGAATG GAATAGAGTC CTTACTGTGT TCTTGGTTAG AAGACACTCC AAATGTGGGC
    CAACAAGTCT CCTTTAAGCA AAAGGAAAAT TCTGCTGCTG CTTTGGTTGC CTTAGCTTGT
    GCTAGGGGAT CATTAAAAAC ATTTATTCAC ACTGTACACT TGCTGCAAAA ACAGACTGAC
    CTAGGATCCC TTCCTGTGGC AGATGTATTG TATAGGTTGC TGCTTTTAGA GGGAGGTCCA
    GGTTCTCCCT CTTGTTTACT GGGAAGCAAA CACATTGTAT CCTGGGGTTT TGAGGATATG
    CTTCCAGCTC CTGATGGGAT CAGTGGTTCA AACAATGAGA ACAAAGATGC AGATTTGGGT
    CGCTGTGTAG CATGCGATGG GCTTTATCTT TACACAACTA ACTCTGTAGG TAGAGGATTG
    AGCAAAGTAG GGTCTGGGCT GCATGGCACT TTACGAGGTT TTGTTTATTC CCGAAACGAG
    GAGCTAGAGG CTGGCTGGAT TGCTTATGGA AGTGGGAAGA TACTTCACAG GCCTGCCTCC
    TTTGATAACA AACCTCAATA TCTTTTCCAA TTAATTGACC AATACACACT TCAGGTCTCT
    TATATAGTCA GCATGCCAGC CAATCATTTC CCGGTTGGCA GCAATATGAC TACGGTGCAC
    CTCTCCTCGG ATGGCACGTA TTTTTACTGG ATTTGGTCTC CAGCTAGTTT AAATGAGAAA
    ACCCCCAAAG GACACTCTGT CTTCATGGAT ATATTTGAGT TAAATGTGCA AAATGGAGTA
    TGCATTGCAA GCCCTCTTCA TGAGCGCATT ATATTAATGA GGAAAGAGGG AGAATCTGCT
    AAAAGTATTA ATGAAATGCT TTTGAGCAGA TTATCACGAT ACAGAGCTTC TCCTTCAGCA
    ACTCTAGCAG CACTTACAGG ATCTACAATC TCTAACACTC TAAAAGAGGA CCAGACTGCT
    GCAAATACGA GCTGCGGGCT TCCTTTGAAA ATGTTAAGAA AGACTCCAAT GTATACTTGT
    GGCACATACT TAGTACTACT CGTGCCACCT CCGGGTGGGT CAGGAAGTTC AGCAACAAGA
    TCTTTGTTTG GTGGAACCAG CGGCCTCTCC TCTTTAAAAA TTCTGGCTTC CAGCTTGGTA
    TTTAACATTT CTGATGGGCA GTTCACCAAC AGAGCAGATC TTATTGATGC ATCTGGAAGT
    TCTCTTGGAA GGGGTGCTTT GGTGACAGGC CTAGGTGCCT GCTATGATAC AATGAATAAC
    ATGATCTGGA CGTGCTCCAA TGATTACATC GATCAGTGGT GTAATCCTGG AAACCAGGCT
    TTCCATGTGG TGTGTCAGAG GCTTGGTGTC ACTCACATCA TTGCTGATCC CAAAGGAGAG
    GCAGTATCAA CAAATGAAGT TATAAACCAG TTGCTTCACC ATGTGGGTGC TATGTGTATT
    CACCAACTCA ATCTCCTGGC TAATAGCAAC AGTTCTCCAA TCAGTACCTT TCTGGGCAAA
    CAACATCCTA TAGAGCCACA TCATCTTAAT AGTATATGTG ACATCATGGA GAAAGCCATG
    GTCAATGGTG ATAAATGCAT TATTCGCTGC ATCTTGGTTG TTTTCCAGGT GGTATTTAAA
    TTCTTTCTAA ACCCACAAAC TGAGAGGAAT CGAGAAACCA TAAGGAGGGC TGGTTTGCTC
    CTTTGGCAGT TGCTCATGGC GCCTAAGGAC CAAATTTGTC CTGAAATACA GAGAGAGGTC
    TGCTTGGCTA TTAGCTTTGG TTTAAACATT TTGAATCCAG GAGAGCTAGA AATTAATAAC
    TTGCTCAAGC TGATTTTAAC TGAAGGGGAC AGAAATTGTG GCCTCTCCCA ACTCCGTGAT
    GTCATTTTGA CAAACCTCAC TGAACAGCTT CAGAATAACA GGTTTGGAAG TGAAGATGAT
    GAACATTACA GGCTTAATGA TGAACTATTG CACTATATTT TGAAGATTGT TGTTCGTGAA
    TCCTGTGTTC TGATCACCAA GTGCCAAACA GTGTCTAAAG AAGAATTCCA AAGACTCTTG
    TCAACAGTGC CTGTGGCTTC TCCCTGTCTA TGCTATTTAA TGGCTGTACA GAATCATCTC
    CTGAGTAACA CTGTCTTAAT TAAACCTGAT GACAGCGATG ACAGTGACAA CTCCCTGCAG
    GGGGAGACAT TGAAGGTACA GGAACTCCAG GCCAGCATCT TGGCTTTGGC AACCCAGATA
    CTTTCTGGTT GTGATGAAGT TCTGGATATT CTGCAGCGAG TGACCACTGC TCTTATCAAC
    AGCGACCTGT TAGACAGAGA TTTAAGACTG AAAGGCCTTG AACAGATCAC AAAGTCCACC
    CTGCTTGGCC ATCTCCTCCC AGTACTTCTC ACATCCCTTA TGCATCCAAA CTTACAGACA
    TTAACCATGG CAGATGCGCT TATGCCTCAG CTGGTGCAGC TTGTTCTTTA CACTAGCCAG
    ACTGCACTGC TGCTTAAAAC CCAGTGTCCA GTATTGGCAG ATACAGGGGA CACCACCAGT
    GGAATTCCAG AGCAAAAAAA CAAACTGTTA ACTGATGACA GGATGCTGGA AGAAAAGGAG
    GAGCCTGGTT TCCTCACTGG GCTAAAGATT CCTGCGCCAT GGGCTGCAGG AAAGACTGTG
    GAAACATCTC ATCCAGTCAG AGACAATTAC AAGTTTAAAG AGACTGTGCA CATTCCTGGA
    GCTCGTTGTT TGTACCTCCG GTTTGACAGC AGGTGCTCTT CACAGTACGA TTATGATAAG
    CTGGTGATAT ATGCGGGACC CAACACAAAC AGCAGAAAAG TTGCCGAATA TGGTGGAAAT
    ACCCTGGGGT ATGGCAGTAG AAGTGTTTTA GGTACAGGCT GGCCAAAAGA TTTGGTTAAG
    GTGGAAGGTG ATACTGTGAC ATTCTCCTTC GAAATGAGAA GTGGGCGTGA GCACAACACT
    CCTGATAAAG CAATGTGGGG TTTTGCGTGC ACTGTCAGAG CTCAGGAGTC CTCAGAAGAT
    GTGTCAGGGG GCTTGCCATT TCTAGTAGAC CTGGCTTTAG GTCTGTCTGT CTTGGCTTGT
    TCCATGCTGC GTATATTGTA CAATGGTCCA GAAATCACAA AAGAAGAGTT GACTTGTCAC
    GATCTGTTGA GGTCAAAGCT TTTGCAGAGG TGTCAGTGGC AGGTGGAGGC TAATGGTGCT
    TTATCCCCGG CACTCACCCC AAGCCCTTCG CCGCTGCCCC TTACTATAGA AGAAGACCAA
    GAATTTACGT ATCCATCTGA CATCTTGTTG CCACCAGTTG GGAACTATTT TGAACTGCCA
    AGAATCAGAC TGCCTCCTGG TATTATGATC AAGCTCAGGG AAATATCTGG ACGCGCTCGA
    CCACAGTTTA GACCAAGCAT AAAAGAGGTT ATCCAGCCTG ATGTGATGGA AGAAATGGTT
    GTATCTTGCG TCATTAAACA CTTGAACCTG GTCGATGCAT TGCAGTCTTT AATAAACTTC
    CAGTATCGAG AAGAACATCT AGAAGAATAT GATTTATTAT GTAAAATAAT GGGGGAAACT
    TTTAAGAAGC TCAATGCAAT GGAAAGACAA TTGCAGAGTG TTGCAGAACT GGAACAAAAG
    TGGCAAAGTG AAGTGGAAGA TGCTCAGAAT GGAAAGCTGG AGAACAACGC TCCCTTTTTT
    TACGACTATC ATTTTAATGA GAGCAAGTTA AAGGAACTTG AACTTTTATG TTCTATGAAA
    GAGGTCCCTT CTGATGGAAG TGATCCAGAA AACATGGTTT TCTCTCTGCG AGAGAAGTTT
    TTTCAAGAGG TGAACTCCCT GATCCAAAAA CCTTCCCACC CAATGGCTAA GACCAAAACA
    TTGGTTAAGA GTCTGATGAA TAGAGCAGAG CTCCTGTTAC ATGTGACTAT TGCAGCACAT
    TCTGGTATCA CGAGAAGCAT ATCTGGCACT CCAACTGAAC CTCAAGCTGG CAAGTCTATC
    TCAGAAGCAA AAGTTATATC TCAAGCCATG CGCCAGCCTG TTTTCCTCCG CAGCATGTCT
    GCCCCTTCTG ACCTGGAAAT GATTGGAAGT AGTGACTTAG AGTTCAGCAG GTCTAATCCA
    AGGAGAAGGA ACATCACTAG CCACAGGAGC AGCTCCTTCA CTCTGCTTCA GTCTTTAGCT
    ATTGAAGACT GCAAGGATAA ACCAACATAC AGTGTCCTTT TAGGGCAACT GTTTGCCTTC
    ATTGGGACCA ATCCCGACCA AGCAGTATCC AGTAGCAGCT TTCTACTTGC AGCCCAAACC
    AGATGGCGTC GTGGTAATAC TCGCAAACAA GCCCTTGTAC ACATGAGAGA ATTACTGACT
    GCTGCTGTCC GTGTTGGGGG TGTGACACAT TTGGTTGGAC CAGTAACAAT GGTGCTACAG
    GGAGGACCAA GAATTGAAGA GCTCACCTGT GGAGGAATGG TGGAGCTTGT TCAAGAGGCA
    TTTGGAGAGA CCATGACCTC AGTGGTGTCA CTTTGTGCAC GGTATCCCAT TGCCTGTGCA
    AATAGCATTG GGCTTTTGTG TACTATACCT TACACAAGGA GTGAAGAAAA ATGTTTAGTT
    CGCAGTGGGC TTGTTCAACT GATGGACCGA CTCTGCAGCT TAAGCAACCA GAGAGAGTCC
    AGCTCAAATG AAAAGCAAAC CAAGAAACAG AAAGTAGCAA CTATGGCATG GGCAGCCTTC
    CAAGTACTGG CCAATAGATG TGTAGAATGG GAGAAAGAAG AAGGTGGTTC TACTGAGGCG
    GTACACTCTG GTTTGGCTAG ACAAGTCTCC ACCCTCCTCA CTAACCATCT AGCAAGGGCT
    ACAGAGTGCT GTGGAAACCA AGCTGCAGGG AATGATGCCC TACAGGACGT TCTGAGCCTT
    CTCAATGATC TCTCTAGGAG TCAGATAGGG AAGGCAATTC TGAGCCAGCC TGCTTGTGTG
    TCCAAACTTC TTTCGTTGCT GCTGGACCAG CGTCCTTCTC CAAAGCTTGT GCTCATAATT
    CTGCAGCTGT GCCGCGCTGC ACTTCCTCTC ATGAGTGTTG AAGACTGTGG AAATGTAGAA
    CTTCCTCCAT GGAGTTACTC TTTACCAGCT CTGGACAGTG AACAGGGGGA GGATCCAAGT
    GACCCAGCCT CCAAAATAGC ATCTTTGTTG CTAGCAAAGT TGGCTGATTA TGTTGTTCCA
    GGATGTCAGA CAGTTCTTTC TCCTACTGCT TCTGAACCAG ACAGCTCATT GTCCAAAAAC
    AGCCCAAAAA ATTCCCAGAA AGCAGATAAG GATGCAGAAG AAGAGAGTGA AGCAGTAGAT
    GGCAAGCTCT CCATTTTTAT TCATAAGCGA GAAGACCAGT CATCCCATGA GGTTCTTCAG
    CCTTTGCTTA GTAGTTCAGA GGGAAGGCCT TTTCGTCTTG GTACTGGAGC GAATATGGAA
    AAAGTAGTAA AGATGGATCG TGACATGACT AAGAGTGGAT GTTGTGAAGT AATCACAGAG
    GAGGCTGCAG CTGCTCTCCG CAAAGCCACT AAATGGGCCC AGTCGGGTTT AATTGTTAGC
    ATTGGGCCAC CTGTGGAGAC AGTTAGCCCT GATTCAGCGA GTGGACTTTC CACTGGTGAC
    AAAAAGAAAA CAGCCCAGAC CTCGATCTGT AGGGAAAGGA ATTCTGAACT TGCCAGGACT
    GATCCTGTAC GCCCTTTCAT TAGTGGCCAT GTAGCAAACA GCATGGCTGC AGAAGTAATT
    GCTCTGCTTC ACAGCCTGCT GATGGCACCA GAGTCAAATG CTGCCCAAAT CTGGACAACA
    ACAGCAGAGA AGGTTCTTTC TCGTGCCCTG ATGTATATCC CACAGCTTGG CAAATATGCA
    GAAAGCATCC TAGAGAACGG CAGCGGTAAT GGCAGGAAGC TAGCAAAGCT ACAAGGCATT
    GCAAGGCAAG CAGTTGCTGC ATTATGTGCG CTTGGTGGAT TTAAAGAAAC CATTAAAATA
    GGTTCTGAAG TCCAGGTGTT GGGTAAAGGA ATATCTGGGA GCATTGGAGT GGTTGTTTCT
    ATTAATGAAC AAGAAGGTAT TGCAACAGTG AAATTTCCAG ACATAGATTT TAGAAAATCA
    TGCAAAGCAT CTGACACACT GACAGTACCA TTATTTAGGC TCTGCGTTCC ACGGTCAGAG
    GCATTGCCTC TCCATAAACT GTCCATTACT GAGAAGGTAG TGCAGGCAGT CCAGTCCATG
    TTACTTCCCC AGGAGGGCTG CCTATCTATT CATACCTCGC TTCCTGCAAC AGGAGATGCC
    TCAACTCCAG TAATGGCAGT GGTTCGACTT CTTGCAGAGA TCAGGACAAG AGCTTGCTTG
    GTGATGGCTC AGCTGTTGGA GGACAGCTCA TTTTGTGAAG AATTCATACA GCAATGTCCA
    GCTGCTGTAG AAGTTCTAAA CCTAGTGGCC CAGGAATGCA GTCCTGGGGA GCGTCTCTCA
    GTGGTGGAAG TTCAGTGTGA AAGACTACGA ATGCTTTATC GGGACTGTGC TCGCCCCCCG
    CCACCACCTT TGCAGGCTGA GCGCAGACAG CCCAAGGAAA TCACCTGGTC TCCTTCCAGG
    GTATTCCCTC CTGTGAGAGC CTGTATGTTT TCATCCCATC TAACAGCAGT AACATTTCTG
    GCTGACCCAA GTGCTGGTGG GGGACTTCCT CGCGGCACAT TTATATATGC AACATCCCCT
    GTTCCAGTAC AGGCACCATC TTTTTACTGG GAAATAGAAG TAGTTTCCTA TGGAGACACA
    GATGATGACA CTGGCCCTAT TGTATCATTT GGGTTTGCTA CAGAAGCAGA GAAGCGGGAT
    GGAGCATGGA CAAACCCAGT CGGTACCTGT CTTTTTCATA ACAATGGTCG AGCTGTACAT
    TACAATGGAT CAAGTCTCTT ACAGTGGAAG AGTGTTCGAT TAGATGTGAC TCTTGCTCCT
    GGTGATGTTG CTGGAATAGG TTGGGAGAGA ACTGAAGGAA CACCACCACC CCCAGGGCAG
    CCTGCCAAGG GCAGAGTCTA CTTTACTTAC TGTGGACAGA GGCTTACTCC GTTCTTAGAG
    GATGTCTCTG GGGGCATGTG GCCAGTTGTG CATATTCAGA AAAAGAACAC TAAAGTCCGT
    GCAAATTTTG GATGCAGACA GTTTGCTTTT GCCGAGGGTC AAGCTCATCG CAATGCAGCA
    GATTTGTGCA TTGATTTGGC TGAAGAGATA AGTGCAAACT TTGAAGCATT GCCCTTTGCT
    ATGGCATCTG ACAGTGATAA TGATGCTGGG ACAAGCATAG CATCTGATCC TGGAACTCAT
    GGACCTCCTT GCCGTATTGC TGCAGTTTCT ACTGTGCAAC AGCAATATGA CAGTGATGCC
    TCATGCCGTT ACAAGGTAGA GCTGAGTTAT CAAGACTTTG TGACCTCTGG ACCTGATCCA
    CACCCACCTC CCATTGCAGA TGATGAAAGT GATGATGATG ACGATGATGA TATTCCTCGA
    GAGGATCATT ATGCTCTTTT GGTAAAAGCA TGGGAGACCA AAGTTTTTCC AACCATTCGA
    AGACGTTTCC GTAACGAGGC AGAGAGAAAG TCTGGCCTGG ACCAGATTAA GGGAGCTCTT
    CAGCTAGGAA TGGTGGATAT AGCGAGGCAA ACTGTAGAGT TCCTCTATGA AGAAAATGGT
    GGCATTCCAA GAGATCTCTA TCTTCCTACT ATTGAGGATA TCAAGGATGA GGCAAATAAA
    TTCACCATTG ATAAAGTTCG CAAAGGACTC ACAGTGGTCA TTCGATCCCC AGATAGCAAC
    AATGTAGCTG GGACAACTGT GGGAACAGCT TTACCCAAGT TTGCCATCCG TGGTATGCTG
    AAGACATTCT CTCTACATGG GGTGGTTCTG GATGTGGATT CAGTTAATGA GCTGGTTCAG
    GTAGAGACTT ACCTCCGGAG TGAAGGGGTA TTAGTGAGAT ACTGGTATCC CATAGATATG
    CTAGAAAGAC CTCCTGCGGG ATACAGGAGA ATGGCAACCA ATGGACTGGT GACTTTAGAT
    AGCACTAACA TCCAGATTCA TAGAGAGCTG TTGCGGTGTG AGTCTGCACT TGCCAGGCTT
    TACTGTCGTG TGGCTTTGCT TAACATCTTT GCTCCTAAGT CTCCGCACAC TTTCACTCGC
    CTGTTCCATA TTCCTGCTAT CCGAGACATT ACATTGGAGC ATTTACAGCT TCTGTCAAAT
    CAGCTTCTAA TGCCACCCCT TCCAGACGGA ACCATTACCT CCAGCTCTAT CCTTCTTGCC
    CAGTCCTTGC AACATTGCAT CTACTCCCAG AACTGCTCAA CAACAGATTT GTTCTACCAG
    GGCAATGCAC AGCCAATAAG AGAGTGGCTT GGTGTGGCCA TAACCAGGGC CCTGCATCAA
    GGAGAGGACA GTCTCTTGGA TCTGACAAAA CAAATCTGTT CCTTCTTGCA AAATGCGCCG
    GAACAGTTTC CATCTGAAGA ATTTCCCATC TCTGAATCCA AGCTCAGTAT GGATGTTAAT
    TTTCCTGGGG CAGCTTTTGT TATTGTTTCT TGTATAGAGA GTCCGTCTGG ATTTAGAAAA
    GACTCCTCCT TGTACAAGGC TCCCTGGTCT AGAGTCATGG TATATGGCCT TGGACATAAA
    GTAAAGAGGA ATGGACAGCT GAACCTGATA GAATCAGTTT GTTATCCACG TGATGCCTCC
    CCAAGTAACA CAGGCCTCAC TCCCCCACCA ACCACAAACC AGTACCCAAC AGCGATTATT
    CCCACTGACA AGGTCCACGT CAAACTTGGT GTATCACCTA CTCCTGGTGC TGTGCTGGTC
    ATCCATTCCC TGCCTCTAGA GTTTCCGTTG GCAATGGCAT TCACAGAACA GCTGTTAACC
    TGGAAGTTGG AGGACACAGA AGAGAAGTCA GAAGATGAGC TTGATACTGT TCCATCCTCT
    GCTCTGTTGC AAGTGGTAGA ACTTTTAGGT ACTTTGATTT GGACAACAGA CCTTGCTGCA
    GTAATTAAGG AGCTTCTGTT CCATCTGTTG GCTGAACTAT TAAGGAAAGT TCACCATCTA
    GAGCAGAAGA AGAACCCAAG TGGATTATCT TCCTCGATAG CCCTGCAGTT GAATCCATGT
    CTAGCAATGC TTATGGCTTT ACAGACAGAA CTGCGCAAGC TGTATGATGA AGAAACTCAA
    AACTGGGCAT CTGGGAATGC ATGTGGGGGG TCTGTACCTG GAGCCTCTGA GCAGGGAAGA
    TTTTCAACTT ACTTTCATGC CCTCATGGAA GTGTGCCTGG CAGTGGCAGA AGTGACGCTC
    CCAATCAACA TGAGCTCAGC AATCAGTGTG ATGGCATCTA CAAATGCCAC TAATCTGAGT
    GACTCTTCTT CTTCCTCTTC CTCTTCCCCT GGACAGACTC CTCAAAGTCC AAGTCTGTTA
    TCAAAGAGAA AGAAAGTTAA GATCAAACGT GAGAAGACTG CATCTGGAAA ACGTACACCT
    TCACGTGGTG CAGAGAGTGA TGCTGCTTTG CTTAGCATTG GAGGAAGCAA ACCTGAGGAC
    ATGTTGTGGT TCCATAGGGC ATTGACTTTA TTGATGATCC TAAGGCATCT AACTAACAAG
    GATCCACAGG GGATGGGTGT CACAAACGAT GCTATTGCTG ATGCTAGCCA GGCTTTAGTT
    GGTCCCACAG CTCACAGTCG TCTCCTTGTG ATTTCTGGCA TCCCTACACA CCTAGAAGAA
    TCAGTGGTCA GAAGTGCAAT TAGAAAGGCA TGTAATGCTC ATGGTGGAGT CTTTAAAGAT
    GAAATCTACA TTCCATTGCA GGAAACTGAA ACCCCTAAAC CCAAAGACAG AATAGAAGGG
    GCAGAATGCA AGACAGAAAT AGAAAAACCC TCTACGCAGC ATACAACCGA CAATATGGAT
    ATCAGCAGCT CGAGCAGTGT AACTCCTGCA GCAAGTGTCA CGGCATCAGC TTCTACAAGC
    CAAGCTTCAT TGTGCAGCTC CCAAGGGGGG GTATCACGGA CCACAAGTGA AACATCTATG
    GAGCAGTTTG CCCCAGAACA TGCTGTGCCA TCAGGGCTTC TGGAACCAAC TATTATCTCC
    AGCCAGGAGA GTCTGGATCT TTCTATGTGC AGCACAGGAA GCCTCGGCAG TCTGGGAAGT
    CTTGTTGAAC AGCTTGATAA TGTTGAAAAC TCTTTGTCTG ACGTGGGCTC CCTATACACA
    GCTACATCAC TAGATAGCCA TATGGCAGAG TCGCGGCCAA TCAAAGGATT TGCAGTTGTA
    GAGGTGCGAT CACGAGCTAA AATAGAGAAA ATCCGTGCCA CTTTATTTAA CAGTAGTGAC
    CTCATTGGGT TATCTGGTTT GGATGGTGAA GATGAGCTGA TGGAAATGTC TACTGAGGAG
    ATCTTGACTG TTTCAACAGT GAATCAGAGC TTGTTTGATA CCCAAGGAAT ACCCGGTCTG
    GCTGATTATT TCATGGATAA ATCAATGAAA GGTGATAAAT TAGTCCCGGA AGCTCGTGAT
    GTTCTGACTG AGATTTTCAA GAGCTGCATT CACGCCGAGC AGATGCTGAG TCTGACTCCA
    GCCAAGTTTA TCAAAGTGTC TGATATCTAC CTTAGCAAGG AGCAAATCAA TTCACAGACC
    CCAGGGAACT TACTGCATAC CTTTTTCACC AATGTTCGAC CACCTAAAAA AGTCCTGGAA
    GATCAACTAA CGCAGATCTT AAGAAAATAT GGAATTCCCA AGCCTAAATT TGACAAAAGC
    AAATACAGCA AAACTGGGAA GGAGCAACAT CCTGTGAAAG TTGTAAGCAC CAAAAGACCT
    GTGGGCAAAC CACCGACCAA AGACAAGGCT GTATTCAACA GCGTCAGGAC TGCTTTAAGT
    GAGAAAAAAC CAACTGTGAA GCCAAAATCT CCGGAGAAGG GCAGGCCAGA TGAGAAGGAC
    CCAGACAAAT CTCCAACAAA GAAGCAAGAA ATTCCCGAGG AGAGATATCT GACGTTGGAT
    GGATTTCACC GCTTTGTAGT AGATCGGGCT AAGCAAGACA TTCGAAGCAT CTGGAGAGCT
    GTCTTGGCGT GTGGATATGA TCTTCATTTT GAAAGGTGTG CCTGCATTGA TACAAGGCAT
    GCTCAGAAAT CTAGCAGAAA ATGGACCTTG GAAATGGATG TTGCTCTTGT TCAGTATATC
    AACCGCCTTT GCCGTCACCT TGCCATAACG CCAGCCCGAC TACACCCTCA TGAGGTTTAT
    CTGGATCTTG CAGATACGGC TGACCCAAGA GTCTCCTGCC TGCTGAATGT ACCAATAGAA
    AGCCTGCGCC TCCGATTTGC TCTTCTTCAG TCCCTCAACA CTACTTTAGA GACCTTTTTC
    CTGCCCTTAG TGGAGCTACG GCAAACAGAC GTATACACAC ACAGCATCGC ATCCCTACTC
    CAGCTAGCTA AAGGGTTAAT CTTTTACGAT ACAAAAGTGA ATGTGATGAA CCGGGTATTG
    AACGCAACAG TGCAGAGGAC AGCAGATCAT GCAGCCCCTG AGATCACGCT GGACCCACTA
    GAAATTGTAG GAGGGGAAAT TCGTACCTCA GAAAACTCCT ACTTCTGCCA GGCAGCCCGA
    CAGTTGGCAT GCATCCCTTC CTCACAACTG TGTGTCAAAC TGGCAAGTGG AGGAGACCCC
    ACGTATGCAT TTAACATCCG CTTTACTGGA GAGGAGGTGC ATGGTACAAG TGGCTCATTC
    CGTCATTTCC TCTGGCAAGT GTGTAAAGAA CTACAGAGCT CCTCGCTGTC TCTTCTACTG
    CTCTGCCCCA GCTCTGCAGT TAATAAGAAT AAGGGAAAAT ATCTTCTAAC TCCCAGTCCC
    ATAACATATG CAGAAGAGCA GTTGCTCCAT TTCTTTGGCC AACTACTGGG AATAGCCATC
    AGAGCCGATG TTCCACTCCC TCTGGATCTA CTCCCCTGCT TCTGGAAGAT GCTTGTAGGA
    GAACCTTTGG ACCCAGAAAC TGATCTTTAT GAAGCAGATA TTCTCACCCA CAACTACATC
    AAGAAGTTTG AAAATGTAAA TGATGAGTCA GAGCTGGAAG CTCTTTGTGC TGAAATTGCC
    TCTCAGCATC TTCCTACCGA AAGTCCAGAT TCTCCTCATA AACCAGCCTG CAAATTTACA
    TACTTGACAA TGACTGGTGA AGAAGTGGAG CTGTGCCCTA AGGGAAGACA CATTTCTGTA
    GTCTGGGAAA ACAAAGATAT ATATGCAATG GGAATCCGGA ACCTTCGTAT ACGTGAGCTG
    AAGAACCAGG AGTGTATGAG CGCAGTAAGG GCTGGGCTGG GCTCCATTAT ACCTCTGCAG
    CTCCTCACCA CTCTAAATGC TCTAGAGATG GAACTAAGGA CATGTGGCCT ACCATATATT
    AATTTGGAAT TCTTAAAGGC TCACACCATG TATCAAGTGG GTCTAATGGA GACAGATCAA
    CACATTGAGT ATTTTTGGTC TGCCTTAGAA ATGTTCACTC AGGAAGAACT TTGCAAATTC
    ATAAAATTCG CCTGCAATCA GGAACGCATT CCCTTTACAT GTCCATGCAA AGATGGTGGG
    CCAGACACGG CTCATGTTCC ACCTTATCCT ATGAAAATAG CACCACCCGA TGGAGCAACA
    GGCTCCCCAG ATTCCAGGTA CATCCGAGTG GAGACATGTA TGTTCATGAT CAAGCTTCCG
    CAGTACTCGT CTCTAGAGAT GATGCTGGAG AAACTGAGAT ATGCCATTCA CTACAGGGAA
    GACCCACTCA GCGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa49027
    Clone ID Related Accession (Same CDS sequence) XM_004910504.4
    Accession Version XM_004910504.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13212bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product probable E3 ubiquitin-protein ligase HECTD4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004910504.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGGGGTCGG CGGCTGCCGG GGACTCGGCT CAGTGGCTGT CGGTGAAAGA AGAGACTATC 
    TTTTTACATG ATGGGCTGAT CCGGGTCACA GACCTGGCGG AGTTGCCCAG CGAAATCGGG
    GTCAGTGAGG CCGGGGACGC AGAACTGGAG ATATTGACTT TTGAGACTAA GAACCCTATT
    GAGCTAGCAG AGCATTTACG TGCAGTGTGT GGGAACCAGA GCAATGCCTA TGCACGATTG
    CTGGAATACC GCTTGAATGC GTTACGTGGG TTATGGAATG CCCAACGCCA GCTGGCGCTT
    GAGGATCAAC ATGAAAGAGA TGGGGCTGAT GACGAAACCT TGGCCCTGCT TAAACGGCAG
    GGTTTGTTGC AACAGCCGGA ACAGGCTCCA TTTACATCTC GAATGGGGCT TTTACTGGTG
    TTCCCCCTTA TCCAGTCCCA AAGTAAAACA GACCCTTCAC TTTGTAACTT GACAGCTGAG
    GTGCTTTTAA ATTGCCTTCG GGACTGCCAA CCCCTGAGCT TGACTAAAGA ACCAGCTGAC
    TGCCTGAATG GAATAGAGTC CTTACTGTGT TCTTGGTTAG AAGACACTCC AAATGTGGGC
    CAACAAGTCT CCTTTAAGCA AAAGGAAAAT TCTGCTGCTG CTTTGGTTGC CTTAGCTTGT
    GCTAGGGGAT CATTAAAAAC ATTTATTCAC ACTGTACACT TGCTGCAAAA ACAGACTGAC
    CTAGGATCCC TTCCTGTGGC AGATGTATTG TATAGGTTGC TGCTTTTAGA GGGAGGTCCA
    GGTTCTCCCT CTTGTTTACT GGGAAGCAAA CACATTGTAT CCTGGGGTTT TGAGGATATG
    CTTCCAGCTC CTGATGGGAT CAGTGGTTCA AACAATGAGA ACAAAGATGC AGATTTGGGT
    CGCTGTGTAG CATGCGATGG GCTTTATCTT TACACAACTA ACTCTGTAGG TAGAGGATTG
    AGCAAAGTAG GGTCTGGGCT GCATGGCACT TTACGAGGTT TTGTTTATTC CCGAAACGAG
    GAGCTAGAGG CTGGCTGGAT TGCTTATGGA AGTGGGAAGA TACTTCACAG GCCTGCCTCC
    TTTGATAACA AACCTCAATA TCTTTTCCAA TTAATTGACC AATACACACT TCAGGTCTCT
    TATATAGTCA GCATGCCAGC CAATCATTTC CCGGTTGGCA GCAATATGAC TACGGTGCAC
    CTCTCCTCGG ATGGCACGTA TTTTTACTGG ATTTGGTCTC CAGCTAGTTT AAATGAGAAA
    ACCCCCAAAG GACACTCTGT CTTCATGGAT ATATTTGAGT TAAATGTGCA AAATGGAGTA
    TGCATTGCAA GCCCTCTTCA TGAGCGCATT ATATTAATGA GGAAAGAGGG AGAATCTGCT
    AAAAGTATTA ATGAAATGCT TTTGAGCAGA TTATCACGAT ACAGAGCTTC TCCTTCAGCA
    ACTCTAGCAG CACTTACAGG ATCTACAATC TCTAACACTC TAAAAGAGGA CCAGACTGCT
    GCAAATACGA GCTGCGGGCT TCCTTTGAAA ATGTTAAGAA AGACTCCAAT GTATACTTGT
    GGCACATACT TAGTACTACT CGTGCCACCT CCGGGTGGGT CAGGAAGTTC AGCAACAAGA
    TCTTTGTTTG GTGGAACCAG CGGCCTCTCC TCTTTAAAAA TTCTGGCTTC CAGCTTGGTA
    TTTAACATTT CTGATGGGCA GTTCACCAAC AGAGCAGATC TTATTGATGC ATCTGGAAGT
    TCTCTTGGAA GGGGTGCTTT GGTGACAGGC CTAGGTGCCT GCTATGATAC AATGAATAAC
    ATGATCTGGA CGTGCTCCAA TGATTACATC GATCAGTGGT GTAATCCTGG AAACCAGGCT
    TTCCATGTGG TGTGTCAGAG GCTTGGTGTC ACTCACATCA TTGCTGATCC CAAAGGAGAG
    GCAGTATCAA CAAATGAAGT TATAAACCAG TTGCTTCACC ATGTGGGTGC TATGTGTATT
    CACCAACTCA ATCTCCTGGC TAATAGCAAC AGTTCTCCAA TCAGTACCTT TCTGGGCAAA
    CAACATCCTA TAGAGCCACA TCATCTTAAT AGTATATGTG ACATCATGGA GAAAGCCATG
    GTCAATGGTG ATAAATGCAT TATTCGCTGC ATCTTGGTTG TTTTCCAGGT GGTATTTAAA
    TTCTTTCTAA ACCCACAAAC TGAGAGGAAT CGAGAAACCA TAAGGAGGGC TGGTTTGCTC
    CTTTGGCAGT TGCTCATGGC GCCTAAGGAC CAAATTTGTC CTGAAATACA GAGAGAGGTC
    TGCTTGGCTA TTAGCTTTGG TTTAAACATT TTGAATCCAG GAGAGCTAGA AATTAATAAC
    TTGCTCAAGC TGATTTTAAC TGAAGGGGAC AGAAATTGTG GCCTCTCCCA ACTCCGTGAT
    GTCATTTTGA CAAACCTCAC TGAACAGCTT CAGAATAACA GGTTTGGAAG TGAAGATGAT
    GAACATTACA GGCTTAATGA TGAACTATTG CACTATATTT TGAAGATTGT TGTTCGTGAA
    TCCTGTGTTC TGATCACCAA GTGCCAAACA GTGTCTAAAG AAGAATTCCA AAGACTCTTG
    TCAACAGTGC CTGTGGCTTC TCCCTGTCTA TGCTATTTAA TGGCTGTACA GAATCATCTC
    CTGAGTAACA CTGTCTTAAT TAAACCTGAT GACAGCGATG ACAGTGACAA CTCCCTGCAG
    GGGGAGACAT TGAAGGAACT CCAGGCCAGC ATCTTGGCTT TGGCAACCCA GATACTTTCT
    GGTTGTGATG AAGTTCTGGA TATTCTGCAG CGAGTGACCA CTGCTCTTAT CAACAGCGAC
    CTGTTAGACA GAGATTTAAG ACTGAAAGGC CTTGAACAGA TCACAAAGTC CACCCTGCTT
    GGCCATCTCC TCCCAGTACT TCTCACATCC CTTATGCATC CAAACTTACA GACATTAACC
    ATGGCAGATG CGCTTATGCC TCAGCTGGTG CAGCTTGTTC TTTACACTAG CCAGACTGCA
    CTGCTGCTTA AAACCCAGTG TCCAGTATTG GCAGATACAG GGGACACCAC CAGTGGAATT
    CCAGAGCAAA AAAACAAACT GTTAACTGAT GACAGGATGC TGGAAGAAAA GGAGGAGCCT
    GGTTTCCTCA CTGGGCTAAA GATTCCTGCG CCATGGGCTG CAGGAAAGAC TGTGGAAACA
    TCTCATCCAG TCAGAGACAA TTACAAGTTT AAAGAGACTG TGCACATTCC TGGAGCTCGT
    TGTTTGTACC TCCGGTTTGA CAGCAGGTGC TCTTCACAGT ACGATTATGA TAAGCTGGTG
    ATATATGCGG GACCCAACAC AAACAGCAGA AAAGTTGCCG AATATGGTGG AAATACCCTG
    GGGTATGGCA GTAGAAGTGT TTTAGGTACA GGCTGGCCAA AAGATTTGGT TAAGGTGGAA
    GGTGATACTG TGACATTCTC CTTCGAAATG AGAAGTGGGC GTGAGCACAA CACTCCTGAT
    AAAGCAATGT GGGGTTTTGC GTGCACTGTC AGAGCTCAGG AGTCCTCAGA AGATGTGTCA
    GGGGGCTTGC CATTTCTAGT AGACCTGGCT TTAGGTCTGT CTGTCTTGGC TTGTTCCATG
    CTGCGTATAT TGTACAATGG TCCAGAAATC ACAAAAGAAG AGTTGACTTG TCACGATCTG
    TTGAGGTCAA AGCTTTTGCA GAGGTGTCAG TGGCAGGTGG AGGCTAATGG TGCTTTATCC
    CCGGCACTCA CCCCAAGCCC TTCGCCGCTG CCCCTTACTA TAGAAGAAGA CCAAGAATTT
    ACGTATCCAT CTGACATCTT GTTGCCACCA GTTGGGAACT ATTTTGAACT GCCAAGAATC
    AGACTGCCTC CTGGTATTAT GATCAAGCTC AGGGAAATAT CTGGACGCGC TCGACCACAG
    TTTAGACCAA GCATAAAAGA GGTTATCCAG CCTGATGTGA TGGAAGAAAT GGTTGTATCT
    TGCGTCATTA AACACTTGAA CCTGGTCGAT GCATTGCAGT CTTTAATAAA CTTCCAGTAT
    CGAGAAGAAC ATCTAGAAGA ATATGATTTA TTATGTAAAA TAATGGGGGA AACTTTTAAG
    AAGCTCAATG CAATGGAAAG ACAATTGCAG AGTGTTGCAG AACTGGAACA AAAGTGGCAA
    AGTGAAGTGG AAGATGCTCA GAATGGAAAG CTGGAGAACA ACGCTCCCTT TTTTTACGAC
    TATCATTTTA ATGAGAGCAA GTTAAAGGAA CTTGAACTTT TATGTTCTAT GAAAGAGGTC
    CCTTCTGATG GAAGTGATCC AGAAAACATG GTTTTCTCTC TGCGAGAGAA GTTTTTTCAA
    GAGGTGAACT CCCTGATCCA AAAACCTTCC CACCCAATGG CTAAGACCAA AACATTGGTT
    AAGAGTCTGA TGAATAGAGC AGAGCTCCTG TTACATGTGA CTATTGCAGC ACATTCTGGT
    ATCACGAGAA GCATATCTGG CACTCCAACT GAACCTCAAG CTGGCAAGTC TATCTCAGAA
    GCAAAAGTTA TATCTCAAGC CATGCGCCAG CCTGTTTTCC TCCGCAGCAT GTCTGCCCCT
    TCTGACCTGG AAATGATTGG AAGTAGTGAC TTAGAGTTCA GCAGGTCTAA TCCAAGGAGA
    AGGAACATCA CTAGCCACAG GAGCAGCTCC TTCACTCTGC TTCAGTCTTT AGCTATTGAA
    GACTGCAAGG ATAAACCAAC ATACAGTGTC CTTTTAGGGC AACTGTTTGC CTTCATTGGG
    ACCAATCCCG ACCAAGCAGT ATCCAGTAGC AGCTTTCTAC TTGCAGCCCA AACCAGATGG
    CGTCGTGGTA ATACTCGCAA ACAAGCCCTT GTACACATGA GAGAATTACT GACTGCTGCT
    GTCCGTGTTG GGGGTGTGAC ACATTTGGTT GGACCAGTAA CAATGGTGCT ACAGGGAGGA
    CCAAGAATTG AAGAGCTCAC CTGTGGAGGA ATGGTGGAGC TTGTTCAAGA GGCATTTGGA
    GAGACCATGA CCTCAGTGGT GTCACTTTGT GCACGGTATC CCATTGCCTG TGCAAATAGC
    ATTGGGCTTT TGTGTACTAT ACCTTACACA AGGAGTGAAG AAAAATGTTT AGTTCGCAGT
    GGGCTTGTTC AACTGATGGA CCGACTCTGC AGCTTAAGCA ACCAGAGAGA GTCCAGCTCA
    AATGAAAAGC AAACCAAGAA ACAGAAAGTA GCAACTATGG CATGGGCAGC CTTCCAAGTA
    CTGGCCAATA GATGTGTAGA ATGGGAGAAA GAAGAAGGTG GTTCTACTGA GGCGGTACAC
    TCTGGTTTGG CTAGACAAGT CTCCACCCTC CTCACTAACC ATCTAGCAAG GGCTACAGAG
    TGCTGTGGAA ACCAAGCTGC AGGGAATGAT GCCCTACAGG ACGTTCTGAG CCTTCTCAAT
    GATCTCTCTA GGAGTCAGAT AGGGAAGGCA ATTCTGAGCC AGCCTGCTTG TGTGTCCAAA
    CTTCTTTCGT TGCTGCTGGA CCAGCGTCCT TCTCCAAAGC TTGTGCTCAT AATTCTGCAG
    CTGTGCCGCG CTGCACTTCC TCTCATGAGT GTTGAAGACT GTGGAAATGT AGAACTTCCT
    CCATGGAGTT ACTCTTTACC AGCTCTGGAC AGTGAACAGG GGGAGGATCC AAGTGACCCA
    GCCTCCAAAA TAGCATCTTT GTTGCTAGCA AAGTTGGCTG ATTATGTTGT TCCAGGATGT
    CAGACAGTTC TTTCTCCTAC TGCTTCTGAA CCAGACAGCT CATTGTCCAA AAACAGCCCA
    AAAAATTCCC AGAAAGCAGA TAAGGATGCA GAAGAAGAGA GTGAAGCAGT AGATGGCAAG
    CTCTCCATTT TTATTCATAA GCGAGAAGAC CAGTCATCCC ATGAGGTTCT TCAGCCTTTG
    CTTAGTAGTT CAGAGGGAAG GCCTTTTCGT CTTGGTACTG GAGCGAATAT GGAAAAAGTA
    GTAAAGATGG ATCGTGACAT GACTAAGAGT GGATGTTGTG AAGTAATCAC AGAGGAGGCT
    GCAGCTGCTC TCCGCAAAGC CACTAAATGG GCCCAGTCGG GTTTAATTGT TAGCATTGGG
    CCACCTGTGG AGACAGTTAG CCCTGATTCA GCGAGTGGAC TTTCCACTGG TGACAAAAAG
    AAAACAGCCC AGACCTCGAT CTGTAGGGAA AGGAATTCTG AACTTGCCAG GACTGATCCT
    GTACGCCCTT TCATTAGTGG CCATGTAGCA AACAGCATGG CTGCAGAAGT AATTGCTCTG
    CTTCACAGCC TGCTGATGGC ACCAGAGTCA AATGCTGCCC AAATCTGGAC AACAACAGCA
    GAGAAGGTTC TTTCTCGTGC CCTGATGTAT ATCCCACAGC TTGGCAAATA TGCAGAAAGC
    ATCCTAGAGA ACGGCAGCGG TAATGGCAGG AAGCTAGCAA AGCTACAAGG CATTGCAAGG
    CAAGCAGTTG CTGCATTATG TGCGCTTGGT GGATTTAAAG AAACCATTAA AATAGGTTCT
    GAAGTCCAGG TGTTGGGTAA AGGAATATCT GGGAGCATTG GAGTGGTTGT TTCTATTAAT
    GAACAAGAAG GTATTGCAAC AGTGAAATTT CCAGACATAG ATTTTAGAAA ATCATGCAAA
    GCATCTGACA CACTGACAGT ACCATTATTT AGGCTCTGCG TTCCACGGTC AGAGGCATTG
    CCTCTCCATA AACTGTCCAT TACTGAGAAG GTAGTGCAGG CAGTCCAGTC CATGTTACTT
    CCCCAGGAGG GCTGCCTATC TATTCATACC TCGCTTCCTG CAACAGGAGA TGCCTCAACT
    CCAGTAATGG CAGTGGTTCG ACTTCTTGCA GAGATCAGGA CAAGAGCTTG CTTGGTGATG
    GCTCAGCTGT TGGAGGACAG CTCATTTTGT GAAGAATTCA TACAGCAATG TCCAGCTGCT
    GTAGAAGTTC TAAACCTAGT GGCCCAGGAA TGCAGTCCTG GGGAGCGTCT CTCAGTGGTG
    GAAGTTCAGT GTGAAAGACT ACGAATGCTT TATCGGGACT GTGCTCGCCC CCCGCCACCA
    CCTTTGCAGG CTGAGCGCAG ACAGCCCAAG GAAATCACCT GGTCTCCTTC CAGGGTATTC
    CCTCCTGTGA GAGCCTGTAT GTTTTCATCC CATCTAACAG CAGTAACATT TCTGGCTGAC
    CCAAGTGCTG GTGGGGGACT TCCTCGCGGC ACATTTATAT ATGCAACATC CCCTGTTCCA
    GTACAGGCAC CATCTTTTTA CTGGGAAATA GAAGTAGTTT CCTATGGAGA CACAGATGAT
    GACACTGGCC CTATTGTATC ATTTGGGTTT GCTACAGAAG CAGAGAAGCG GGATGGAGCA
    TGGACAAACC CAGTCGGTAC CTGTCTTTTT CATAACAATG GTCGAGCTGT ACATTACAAT
    GGATCAAGTC TCTTACAGTG GAAGAGTGTT CGATTAGATG TGACTCTTGC TCCTGGTGAT
    GTTGCTGGAA TAGGTTGGGA GAGAACTGAA GGAACACCAC CACCCCCAGG GCAGCCTGCC
    AAGGGCAGAG TCTACTTTAC TTACTGTGGA CAGAGGCTTA CTCCGTTCTT AGAGGATGTC
    TCTGGGGGCA TGTGGCCAGT TGTGCATATT CAGAAAAAGA ACACTAAAGT CCGTGCAAAT
    TTTGGATGCA GACAGTTTGC TTTTGCCGAG GGTCAAGCTC ATCGCAATGC AGCAGATTTG
    TGCATTGATT TGGCTGAAGA GATAAGTGCA AACTTTGAAG CATTGCCCTT TGCTATGGCA
    TCTGACAGTG ATAATGATGC TGGGACAAGC ATAGCATCTG ATCCTGGAAC TCATGGACCT
    CCTTGCCGTA TTGCTGCAGT TTCTACTGTG CAACAGCAAT ATGACAGTGA TGCCTCATGC
    CGTTACAAGG TAGAGCTGAG TTATCAAGAC TTTGTGACCT CTGGACCTGA TCCACACCCA
    CCTCCCATTG CAGATGATGA AAGTGATGAT GATGACGATG ATGATATTCC TCGAGAGGAT
    CATTATGCTC TTTTGGTAAA AGCATGGGAG ACCAAAGTTT TTCCAACCAT TCGAAGACGT
    TTCCGTAACG AGGCAGAGAG AAAGTCTGGC CTGGACCAGA TTAAGGGAGC TCTTCAGCTA
    GGAATGGTGG ATATAGCGAG GCAAACTGTA GAGTTCCTCT ATGAAGAAAA TGGTGGCATT
    CCAAGAGATC TCTATCTTCC TACTATTGAG GATATCAAGG ATGAGGCAAA TAAATTCACC
    ATTGATAAAG TTCGCAAAGG ACTCACAGTG GTCATTCGAT CCCCAGATAG CAACAATGTA
    GCTGGGACAA CTGTGGGAAC AGCTTTACCC AAGTTTGCCA TCCGTGGTAT GCTGAAGACA
    TTCTCTCTAC ATGGGGTGGT TCTGGATGTG GATTCAGTTA ATGAGCTGGT TCAGGTAGAG
    ACTTACCTCC GGAGTGAAGG GGTATTAGTG AGATACTGGT ATCCCATAGA TATGCTAGAA
    AGACCTCCTG CGGGATACAG GAGAATGGCA ACCAATGGAC TGGTGACTTT AGATAGCACT
    AACATCCAGA TTCATAGAGA GCTGTTGCGG TGTGAGTCTG CACTTGCCAG GCTTTACTGT
    CGTGTGGCTT TGCTTAACAT CTTTGCTCCT AAGTCTCCGC ACACTTTCAC TCGCCTGTTC
    CATATTCCTG CTATCCGAGA CATTACATTG GAGCATTTAC AGCTTCTGTC AAATCAGCTT
    CTAATGCCAC CCCTTCCAGA CGGAACCATT ACCTCCAGCT CTATCCTTCT TGCCCAGTCC
    TTGCAACATT GCATCTACTC CCAGAACTGC TCAACAACAG ATTTGTTCTA CCAGGGCAAT
    GCACAGCCAA TAAGAGAGTG GCTTGGTGTG GCCATAACCA GGGCCCTGCA TCAAGGAGAG
    GACAGTCTCT TGGATCTGAC AAAACAAATC TGTTCCTTCT TGCAAAATGC GCCGGAACAG
    TTTCCATCTG AAGAATTTCC CATCTCTGAA TCCAAGCTCA GTATGGATGT TAATTTTCCT
    GGGGCAGCTT TTGTTATTGT TTCTTGTATA GAGAGTCCGT CTGGATTTAG AAAAGACTCC
    TCCTTGTACA AGGCTCCCTG GTCTAGAGTC ATGGTATATG GCCTTGGACA TAAAGTAAAG
    AGGAATGGAC AGCTGAACCT GATAGAATCA GTTTGTTATC CACGTGATGC CTCCCCAAGT
    AACACAGGCC TCACTCCCCC ACCAACCACA AACCAGTACC CAACAGCGAT TATTCCCACT
    GACAAGGTCC ACGTCAAACT TGGTGTATCA CCTACTCCTG GTGCTGTGCT GGTCATCCAT
    TCCCTGCCTC TAGAGTTTCC GTTGGCAATG GCATTCACAG AACAGCTGTT AACCTGGAAG
    TTGGAGGACA CAGAAGAGAA GTCAGAAGAT GAGCTTGATA CTGTTCCATC CTCTGCTCTG
    TTGCAAGTGG TAGAACTTTT AGGTACTTTG ATTTGGACAA CAGACCTTGC TGCAGTAATT
    AAGGAGCTTC TGTTCCATCT GTTGGCTGAA CTATTAAGGA AAGTTCACCA TCTAGAGCAG
    AAGAAGAACC CAAGTGGATT ATCTTCCTCG ATAGCCCTGC AGTTGAATCC ATGTCTAGCA
    ATGCTTATGG CTTTACAGAC AGAACTGCGC AAGCTGTATG ATGAAGAAAC TCAAAACTGG
    GCATCTGGGA ATGCATGTGG GGGGTCTGTA CCTGGAGCCT CTGAGCAGGG AAGATTTTCA
    ACTTACTTTC ATGCCCTCAT GGAAGTGTGC CTGGCAGTGG CAGAAGTGAC GCTCCCAATC
    AACATGAGCT CAGCAATCAG TGTGATGGCA TCTACAAATG CCACTAATCT GAGTGACTCT
    TCTTCTTCCT CTTCCTCTTC CCCTGGACAG ACTCCTCAAA GTCCAAGTCT GTTATCAAAG
    AGAAAGAAAG TTAAGATCAA ACGTGAGAAG ACTGCATCTG GAAAACGTAC ACCTTCACGT
    GGTGCAGAGA GTGATGCTGC TTTGCTTAGC ATTGGAGGAA GCAAACCTGA GGACATGTTG
    TGGTTCCATA GGGCATTGAC TTTATTGATG ATCCTAAGGC ATCTAACTAA CAAGGATCCA
    CAGGGGATGG GTGTCACAAA CGATGCTATT GCTGATGCTA GCCAGGCTTT AGTTGGTCCC
    ACAGCTCACA GTCGTCTCCT TGTGATTTCT GGCATCCCTA CACACCTAGA AGAATCAGTG
    GTCAGAAGTG CAATTAGAAA GGCATGTAAT GCTCATGGTG GAGTCTTTAA AGATGAAATC
    TACATTCCAT TGCAGGAAAC TGAAACCCCT AAACCCAAAG ACAGAATAGA AGGGGCAGAA
    TGCAAGACAG AAATAGAAAA ACCCTCTACG CAGCATACAA CCGACAATAT GGATATCAGC
    AGCTCGAGCA GTGTAACTCC TGCAGCAAGT GTCACGGCAT CAGCTTCTAC AAGCCAAGCT
    TCATTGTGCA GCTCCCAAGG GGGGGTATCA CGGACCACAA GTGAAACATC TATGGAGCAG
    TTTGCCCCAG AACATGCTGT GCCATCAGGG CTTCTGGAAC CAACTATTAT CTCCAGCCAG
    GAGAGTCTGG ATCTTTCTAT GTGCAGCACA GGAAGCCTCG GCAGTCTGGG AAGTCTTGTT
    GAACAGCTTG ATAATGTTGA AAACTCTTTG TCTGACGTGG GCTCCCTATA CACAGCTACA
    TCACTAGATA GCCATATGGC AGAGTCGCGG CCAATCAAAG GATTTGCAGT TGTAGAGGTG
    CGATCACGAG CTAAAATAGA GAAAATCCGT GCCACTTTAT TTAACAGTAG TGACCTCATT
    GGGTTATCTG GTTTGGATGG TGAAGATGAG CTGATGGAAA TGTCTACTGA GGAGATCTTG
    ACTGTTTCAA CAGTGAATCA GAGCTTGTTT GATACCCAAG GAATACCCGG TCTGGCTGAT
    TATTTCATGG ATAAATCAAT GAAAGGTGAT AAATTAGTCC CGGAAGCTCG TGATGTTCTG
    ACTGAGATTT TCAAGAGCTG CATTCACGCC GAGCAGATGC TGAGTCTGAC TCCAGCCAAG
    TTTATCAAAG TGTCTGATAT CTACCTTAGC AAGGAGCAAA TCAATTCACA GACCCCAGGG
    AACTTACTGC ATACCTTTTT CACCAATGTT CGACCACCTA AAAAAGTCCT GGAAGATCAA
    CTAACGCAGA TCTTAAGAAA ATATGGAATT CCCAAGCCTA AATTTGACAA AAGCAAATAC
    AGCAAAACTG GGAAGGAGCA ACATCCTGTG AAAGTTGTAA GCACCAAAAG ACCTGTGGGC
    AAACCACCGA CCAAAGACAA GGCTGTATTC AACAGCGTCA GGACTGCTTT AAGTGAGAAA
    AAACCAACTG TGAAGCCAAA ATCTCCGGAG AAGGGCAGGC CAGATGAGAA GGACCCAGAC
    AAATCTCCAA CAAAGAAGCA AGAAATTCCC GAGGAGAGAT ATCTGACGTT GGATGGATTT
    CACCGCTTTG TAGTAGATCG GGCTAAGCAA GACATTCGAA GCATCTGGAG AGCTGTCTTG
    GCGTGTGGAT ATGATCTTCA TTTTGAAAGG TGTGCCTGCA TTGATACAAG GCATGCTCAG
    AAATCTAGCA GAAAATGGAC CTTGGAAATG GATGTTGCTC TTGTTCAGTA TATCAACCGC
    CTTTGCCGTC ACCTTGCCAT AACGCCAGCC CGACTACACC CTCATGAGGT TTATCTGGAT
    CTTGCAGATA CGGCTGACCC AAGAGTCTCC TGCCTGCTGA ATGTACCAAT AGAAAGCCTG
    CGCCTCCGAT TTGCTCTTCT TCAGTCCCTC AACACTACTT TAGAGACCTT TTTCCTGCCC
    TTAGTGGAGC TACGGCAAAC AGACGTATAC ACACACAGCA TCGCATCCCT ACTCCAGCTA
    GCTAAAGGGT TAATCTTTTA CGATACAAAA GTGAATGTGA TGAACCGGGT ATTGAACGCA
    ACAGTGCAGA GGACAGCAGA TCATGCAGCC CCTGAGATCA CGCTGGACCC ACTAGAAATT
    GTAGGAGGGG AAATTCGTAC CTCAGAAAAC TCCTACTTCT GCCAGGCAGC CCGACAGTTG
    GCATGCATCC CTTCCTCACA ACTGTGTGTC AAACTGGCAA GTGGAGGAGA CCCCACGTAT
    GCATTTAACA TCCGCTTTAC TGGAGAGGAG GTGCATGGTA CAAGTGGCTC ATTCCGTCAT
    TTCCTCTGGC AAGTGTGTAA AGAACTACAG AGCTCCTCGC TGTCTCTTCT ACTGCTCTGC
    CCCAGCTCTG CAGTTAATAA GAATAAGGGA AAATATCTTC TAACTCCCAG TCCCATAACA
    TATGCAGAAG AGCAGTTGCT CCATTTCTTT GGCCAACTAC TGGGAATAGC CATCAGAGCC
    GATGTTCCAC TCCCTCTGGA TCTACTCCCC TGCTTCTGGA AGATGCTTGT AGGAGAACCT
    TTGGACCCAG AAACTGATCT TTATGAAGCA GATATTCTCA CCCACAACTA CATCAAGAAG
    TTTGAAAATG TAAATGATGA GTCAGAGCTG GAAGCTCTTT GTGCTGAAAT TGCCTCTCAG
    CATCTTCCTA CCGAAAGTCC AGATTCTCCT CATAAACCAG CCTGCAAATT TACATACTTG
    ACAATGACTG GTGAAGAAGT GGAGCTGTGC CCTAAGGGAA GACACATTTC TGTAGTCTGG
    GAAAACAAAG ATATATATGC AATGGGAATC CGGAACCTTC GTATACGTGA GCTGAAGAAC
    CAGGAGTGTA TGAGCGCAGT AAGGGCTGGG CTGGGCTCCA TTATACCTCT GCAGCTCCTC
    ACCACTCTAA ATGCTCTAGA GATGGAACTA AGGACATGTG GCCTACCATA TATTAATTTG
    GAATTCTTAA AGGCTCACAC CATGTATCAA GTGGGTCTAA TGGAGACAGA TCAACACATT
    GAGTATTTTT GGTCTGCCTT AGAAATGTTC ACTCAGGAAG AACTTTGCAA ATTCATAAAA
    TTCGCCTGCA ATCAGGAACG CATTCCCTTT ACATGTCCAT GCAAAGATGG TGGGCCAGAC
    ACGGCTCATG TTCCACCTTA TCCTATGAAA ATAGCACCAC CCGATGGAGC AACAGGCTCC
    CCAGATTCCA GGTACATCCG AGTGGAGACA TGTATGTTCA TGATCAAGCT TCCGCAGTAC
    TCGTCTCTAG AGATGATGCT GGAGAAACTG AGATATGCCA TTCACTACAG GGAAGACCCA
    CTCAGCGGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004910561.1
    CDS320..13531
    Translation

    Target ORF information:

    RefSeq Version XM_004910504.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 4 (hectd4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004910504.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGGGGTCGG CGGCTGCCGG GGACTCGGCT CAGTGGCTGT CGGTGAAAGA AGAGACTATC 
    TTTTTACATG ATGGGCTGAT CCGGGTCACA GACCTGGCGG AGTTGCCCAG CGAAATCGGG
    GTCAGTGAGG CCGGGGACGC AGAACTGGAG ATATTGACTT TTGAGACTAA GAACCCTATT
    GAGCTAGCAG AGCATTTACG TGCAGTGTGT GGGAACCAGA GCAATGCCTA TGCACGATTG
    CTGGAATACC GCTTGAATGC GTTACGTGGG TTATGGAATG CCCAACGCCA GCTGGCGCTT
    GAGGATCAAC ATGAAAGAGA TGGGGCTGAT GACGAAACCT TGGCCCTGCT TAAACGGCAG
    GGTTTGTTGC AACAGCCGGA ACAGGCTCCA TTTACATCTC GAATGGGGCT TTTACTGGTG
    TTCCCCCTTA TCCAGTCCCA AAGTAAAACA GACCCTTCAC TTTGTAACTT GACAGCTGAG
    GTGCTTTTAA ATTGCCTTCG GGACTGCCAA CCCCTGAGCT TGACTAAAGA ACCAGCTGAC
    TGCCTGAATG GAATAGAGTC CTTACTGTGT TCTTGGTTAG AAGACACTCC AAATGTGGGC
    CAACAAGTCT CCTTTAAGCA AAAGGAAAAT TCTGCTGCTG CTTTGGTTGC CTTAGCTTGT
    GCTAGGGGAT CATTAAAAAC ATTTATTCAC ACTGTACACT TGCTGCAAAA ACAGACTGAC
    CTAGGATCCC TTCCTGTGGC AGATGTATTG TATAGGTTGC TGCTTTTAGA GGGAGGTCCA
    GGTTCTCCCT CTTGTTTACT GGGAAGCAAA CACATTGTAT CCTGGGGTTT TGAGGATATG
    CTTCCAGCTC CTGATGGGAT CAGTGGTTCA AACAATGAGA ACAAAGATGC AGATTTGGGT
    CGCTGTGTAG CATGCGATGG GCTTTATCTT TACACAACTA ACTCTGTAGG TAGAGGATTG
    AGCAAAGTAG GGTCTGGGCT GCATGGCACT TTACGAGGTT TTGTTTATTC CCGAAACGAG
    GAGCTAGAGG CTGGCTGGAT TGCTTATGGA AGTGGGAAGA TACTTCACAG GCCTGCCTCC
    TTTGATAACA AACCTCAATA TCTTTTCCAA TTAATTGACC AATACACACT TCAGGTCTCT
    TATATAGTCA GCATGCCAGC CAATCATTTC CCGGTTGGCA GCAATATGAC TACGGTGCAC
    CTCTCCTCGG ATGGCACGTA TTTTTACTGG ATTTGGTCTC CAGCTAGTTT AAATGAGAAA
    ACCCCCAAAG GACACTCTGT CTTCATGGAT ATATTTGAGT TAAATGTGCA AAATGGAGTA
    TGCATTGCAA GCCCTCTTCA TGAGCGCATT ATATTAATGA GGAAAGAGGG AGAATCTGCT
    AAAAGTATTA ATGAAATGCT TTTGAGCAGA TTATCACGAT ACAGAGCTTC TCCTTCAGCA
    ACTCTAGCAG CACTTACAGG ATCTACAATC TCTAACACTC TAAAAGAGGA CCAGACTGCT
    GCAAATACGA GCTGCGGGCT TCCTTTGAAA ATGTTAAGAA AGACTCCAAT GTATACTTGT
    GGCACATACT TAGTACTACT CGTGCCACCT CCGGGTGGGT CAGGAAGTTC AGCAACAAGA
    TCTTTGTTTG GTGGAACCAG CGGCCTCTCC TCTTTAAAAA TTCTGGCTTC CAGCTTGGTA
    TTTAACATTT CTGATGGGCA GTTCACCAAC AGAGCAGATC TTATTGATGC ATCTGGAAGT
    TCTCTTGGAA GGGGTGCTTT GGTGACAGGC CTAGGTGCCT GCTATGATAC AATGAATAAC
    ATGATCTGGA CGTGCTCCAA TGATTACATC GATCAGTGGT GTAATCCTGG AAACCAGGCT
    TTCCATGTGG TGTGTCAGAG GCTTGGTGTC ACTCACATCA TTGCTGATCC CAAAGGAGAG
    GCAGTATCAA CAAATGAAGT TATAAACCAG TTGCTTCACC ATGTGGGTGC TATGTGTATT
    CACCAACTCA ATCTCCTGGC TAATAGCAAC AGTTCTCCAA TCAGTACCTT TCTGGGCAAA
    CAACATCCTA TAGAGCCACA TCATCTTAAT AGTATATGTG ACATCATGGA GAAAGCCATG
    GTCAATGGTG ATAAATGCAT TATTCGCTGC ATCTTGGTTG TTTTCCAGGT GGTATTTAAA
    TTCTTTCTAA ACCCACAAAC TGAGAGGAAT CGAGAAACCA TAAGGAGGGC TGGTTTGCTC
    CTTTGGCAGT TGCTCATGGC GCCTAAGGAC CAAATTTGTC CTGAAATACA GAGAGAGGTC
    TGCTTGGCTA TTAGCTTTGG TTTAAACATT TTGAATCCAG GAGAGCTAGA AATTAATAAC
    TTGCTCAAGC TGATTTTAAC TGAAGGGGAC AGAAATTGTG GCCTCTCCCA ACTCCGTGAT
    GTCATTTTGA CAAACCTCAC TGAACAGCTT CAGAATAACA GGTTTGGAAG TGAAGATGAT
    GAACATTACA GGCTTAATGA TGAACTATTG CACTATATTT TGAAGATTGT TGTTCGTGAA
    TCCTGTGTTC TGATCACCAA GTGCCAAACA GTGTCTAAAG AAGAATTCCA AAGACTCTTG
    TCAACAGTGC CTGTGGCTTC TCCCTGTCTA TGCTATTTAA TGGCTGTACA GAATCATCTC
    CTGAGTAACA CTGTCTTAAT TAAACCTGAT GACAGCGATG ACAGTGACAA CTCCCTGCAG
    GGGGAGACAT TGAAGGAACT CCAGGCCAGC ATCTTGGCTT TGGCAACCCA GATACTTTCT
    GGTTGTGATG AAGTTCTGGA TATTCTGCAG CGAGTGACCA CTGCTCTTAT CAACAGCGAC
    CTGTTAGACA GAGATTTAAG ACTGAAAGGC CTTGAACAGA TCACAAAGTC CACCCTGCTT
    GGCCATCTCC TCCCAGTACT TCTCACATCC CTTATGCATC CAAACTTACA GACATTAACC
    ATGGCAGATG CGCTTATGCC TCAGCTGGTG CAGCTTGTTC TTTACACTAG CCAGACTGCA
    CTGCTGCTTA AAACCCAGTG TCCAGTATTG GCAGATACAG GGGACACCAC CAGTGGAATT
    CCAGAGCAAA AAAACAAACT GTTAACTGAT GACAGGATGC TGGAAGAAAA GGAGGAGCCT
    GGTTTCCTCA CTGGGCTAAA GATTCCTGCG CCATGGGCTG CAGGAAAGAC TGTGGAAACA
    TCTCATCCAG TCAGAGACAA TTACAAGTTT AAAGAGACTG TGCACATTCC TGGAGCTCGT
    TGTTTGTACC TCCGGTTTGA CAGCAGGTGC TCTTCACAGT ACGATTATGA TAAGCTGGTG
    ATATATGCGG GACCCAACAC AAACAGCAGA AAAGTTGCCG AATATGGTGG AAATACCCTG
    GGGTATGGCA GTAGAAGTGT TTTAGGTACA GGCTGGCCAA AAGATTTGGT TAAGGTGGAA
    GGTGATACTG TGACATTCTC CTTCGAAATG AGAAGTGGGC GTGAGCACAA CACTCCTGAT
    AAAGCAATGT GGGGTTTTGC GTGCACTGTC AGAGCTCAGG AGTCCTCAGA AGATGTGTCA
    GGGGGCTTGC CATTTCTAGT AGACCTGGCT TTAGGTCTGT CTGTCTTGGC TTGTTCCATG
    CTGCGTATAT TGTACAATGG TCCAGAAATC ACAAAAGAAG AGTTGACTTG TCACGATCTG
    TTGAGGTCAA AGCTTTTGCA GAGGTGTCAG TGGCAGGTGG AGGCTAATGG TGCTTTATCC
    CCGGCACTCA CCCCAAGCCC TTCGCCGCTG CCCCTTACTA TAGAAGAAGA CCAAGAATTT
    ACGTATCCAT CTGACATCTT GTTGCCACCA GTTGGGAACT ATTTTGAACT GCCAAGAATC
    AGACTGCCTC CTGGTATTAT GATCAAGCTC AGGGAAATAT CTGGACGCGC TCGACCACAG
    TTTAGACCAA GCATAAAAGA GGTTATCCAG CCTGATGTGA TGGAAGAAAT GGTTGTATCT
    TGCGTCATTA AACACTTGAA CCTGGTCGAT GCATTGCAGT CTTTAATAAA CTTCCAGTAT
    CGAGAAGAAC ATCTAGAAGA ATATGATTTA TTATGTAAAA TAATGGGGGA AACTTTTAAG
    AAGCTCAATG CAATGGAAAG ACAATTGCAG AGTGTTGCAG AACTGGAACA AAAGTGGCAA
    AGTGAAGTGG AAGATGCTCA GAATGGAAAG CTGGAGAACA ACGCTCCCTT TTTTTACGAC
    TATCATTTTA ATGAGAGCAA GTTAAAGGAA CTTGAACTTT TATGTTCTAT GAAAGAGGTC
    CCTTCTGATG GAAGTGATCC AGAAAACATG GTTTTCTCTC TGCGAGAGAA GTTTTTTCAA
    GAGGTGAACT CCCTGATCCA AAAACCTTCC CACCCAATGG CTAAGACCAA AACATTGGTT
    AAGAGTCTGA TGAATAGAGC AGAGCTCCTG TTACATGTGA CTATTGCAGC ACATTCTGGT
    ATCACGAGAA GCATATCTGG CACTCCAACT GAACCTCAAG CTGGCAAGTC TATCTCAGAA
    GCAAAAGTTA TATCTCAAGC CATGCGCCAG CCTGTTTTCC TCCGCAGCAT GTCTGCCCCT
    TCTGACCTGG AAATGATTGG AAGTAGTGAC TTAGAGTTCA GCAGGTCTAA TCCAAGGAGA
    AGGAACATCA CTAGCCACAG GAGCAGCTCC TTCACTCTGC TTCAGTCTTT AGCTATTGAA
    GACTGCAAGG ATAAACCAAC ATACAGTGTC CTTTTAGGGC AACTGTTTGC CTTCATTGGG
    ACCAATCCCG ACCAAGCAGT ATCCAGTAGC AGCTTTCTAC TTGCAGCCCA AACCAGATGG
    CGTCGTGGTA ATACTCGCAA ACAAGCCCTT GTACACATGA GAGAATTACT GACTGCTGCT
    GTCCGTGTTG GGGGTGTGAC ACATTTGGTT GGACCAGTAA CAATGGTGCT ACAGGGAGGA
    CCAAGAATTG AAGAGCTCAC CTGTGGAGGA ATGGTGGAGC TTGTTCAAGA GGCATTTGGA
    GAGACCATGA CCTCAGTGGT GTCACTTTGT GCACGGTATC CCATTGCCTG TGCAAATAGC
    ATTGGGCTTT TGTGTACTAT ACCTTACACA AGGAGTGAAG AAAAATGTTT AGTTCGCAGT
    GGGCTTGTTC AACTGATGGA CCGACTCTGC AGCTTAAGCA ACCAGAGAGA GTCCAGCTCA
    AATGAAAAGC AAACCAAGAA ACAGAAAGTA GCAACTATGG CATGGGCAGC CTTCCAAGTA
    CTGGCCAATA GATGTGTAGA ATGGGAGAAA GAAGAAGGTG GTTCTACTGA GGCGGTACAC
    TCTGGTTTGG CTAGACAAGT CTCCACCCTC CTCACTAACC ATCTAGCAAG GGCTACAGAG
    TGCTGTGGAA ACCAAGCTGC AGGGAATGAT GCCCTACAGG ACGTTCTGAG CCTTCTCAAT
    GATCTCTCTA GGAGTCAGAT AGGGAAGGCA ATTCTGAGCC AGCCTGCTTG TGTGTCCAAA
    CTTCTTTCGT TGCTGCTGGA CCAGCGTCCT TCTCCAAAGC TTGTGCTCAT AATTCTGCAG
    CTGTGCCGCG CTGCACTTCC TCTCATGAGT GTTGAAGACT GTGGAAATGT AGAACTTCCT
    CCATGGAGTT ACTCTTTACC AGCTCTGGAC AGTGAACAGG GGGAGGATCC AAGTGACCCA
    GCCTCCAAAA TAGCATCTTT GTTGCTAGCA AAGTTGGCTG ATTATGTTGT TCCAGGATGT
    CAGACAGTTC TTTCTCCTAC TGCTTCTGAA CCAGACAGCT CATTGTCCAA AAACAGCCCA
    AAAAATTCCC AGAAAGCAGA TAAGGATGCA GAAGAAGAGA GTGAAGCAGT AGATGGCAAG
    CTCTCCATTT TTATTCATAA GCGAGAAGAC CAGTCATCCC ATGAGGTTCT TCAGCCTTTG
    CTTAGTAGTT CAGAGGGAAG GCCTTTTCGT CTTGGTACTG GAGCGAATAT GGAAAAAGTA
    GTAAAGATGG ATCGTGACAT GACTAAGAGT GGATGTTGTG AAGTAATCAC AGAGGAGGCT
    GCAGCTGCTC TCCGCAAAGC CACTAAATGG GCCCAGTCGG GTTTAATTGT TAGCATTGGG
    CCACCTGTGG AGACAGTTAG CCCTGATTCA GCGAGTGGAC TTTCCACTGG TGACAAAAAG
    AAAACAGCCC AGACCTCGAT CTGTAGGGAA AGGAATTCTG AACTTGCCAG GACTGATCCT
    GTACGCCCTT TCATTAGTGG CCATGTAGCA AACAGCATGG CTGCAGAAGT AATTGCTCTG
    CTTCACAGCC TGCTGATGGC ACCAGAGTCA AATGCTGCCC AAATCTGGAC AACAACAGCA
    GAGAAGGTTC TTTCTCGTGC CCTGATGTAT ATCCCACAGC TTGGCAAATA TGCAGAAAGC
    ATCCTAGAGA ACGGCAGCGG TAATGGCAGG AAGCTAGCAA AGCTACAAGG CATTGCAAGG
    CAAGCAGTTG CTGCATTATG TGCGCTTGGT GGATTTAAAG AAACCATTAA AATAGGTTCT
    GAAGTCCAGG TGTTGGGTAA AGGAATATCT GGGAGCATTG GAGTGGTTGT TTCTATTAAT
    GAACAAGAAG GTATTGCAAC AGTGAAATTT CCAGACATAG ATTTTAGAAA ATCATGCAAA
    GCATCTGACA CACTGACAGT ACCATTATTT AGGCTCTGCG TTCCACGGTC AGAGGCATTG
    CCTCTCCATA AACTGTCCAT TACTGAGAAG GTAGTGCAGG CAGTCCAGTC CATGTTACTT
    CCCCAGGAGG GCTGCCTATC TATTCATACC TCGCTTCCTG CAACAGGAGA TGCCTCAACT
    CCAGTAATGG CAGTGGTTCG ACTTCTTGCA GAGATCAGGA CAAGAGCTTG CTTGGTGATG
    GCTCAGCTGT TGGAGGACAG CTCATTTTGT GAAGAATTCA TACAGCAATG TCCAGCTGCT
    GTAGAAGTTC TAAACCTAGT GGCCCAGGAA TGCAGTCCTG GGGAGCGTCT CTCAGTGGTG
    GAAGTTCAGT GTGAAAGACT ACGAATGCTT TATCGGGACT GTGCTCGCCC CCCGCCACCA
    CCTTTGCAGG CTGAGCGCAG ACAGCCCAAG GAAATCACCT GGTCTCCTTC CAGGGTATTC
    CCTCCTGTGA GAGCCTGTAT GTTTTCATCC CATCTAACAG CAGTAACATT TCTGGCTGAC
    CCAAGTGCTG GTGGGGGACT TCCTCGCGGC ACATTTATAT ATGCAACATC CCCTGTTCCA
    GTACAGGCAC CATCTTTTTA CTGGGAAATA GAAGTAGTTT CCTATGGAGA CACAGATGAT
    GACACTGGCC CTATTGTATC ATTTGGGTTT GCTACAGAAG CAGAGAAGCG GGATGGAGCA
    TGGACAAACC CAGTCGGTAC CTGTCTTTTT CATAACAATG GTCGAGCTGT ACATTACAAT
    GGATCAAGTC TCTTACAGTG GAAGAGTGTT CGATTAGATG TGACTCTTGC TCCTGGTGAT
    GTTGCTGGAA TAGGTTGGGA GAGAACTGAA GGAACACCAC CACCCCCAGG GCAGCCTGCC
    AAGGGCAGAG TCTACTTTAC TTACTGTGGA CAGAGGCTTA CTCCGTTCTT AGAGGATGTC
    TCTGGGGGCA TGTGGCCAGT TGTGCATATT CAGAAAAAGA ACACTAAAGT CCGTGCAAAT
    TTTGGATGCA GACAGTTTGC TTTTGCCGAG GGTCAAGCTC ATCGCAATGC AGCAGATTTG
    TGCATTGATT TGGCTGAAGA GATAAGTGCA AACTTTGAAG CATTGCCCTT TGCTATGGCA
    TCTGACAGTG ATAATGATGC TGGGACAAGC ATAGCATCTG ATCCTGGAAC TCATGGACCT
    CCTTGCCGTA TTGCTGCAGT TTCTACTGTG CAACAGCAAT ATGACAGTGA TGCCTCATGC
    CGTTACAAGG TAGAGCTGAG TTATCAAGAC TTTGTGACCT CTGGACCTGA TCCACACCCA
    CCTCCCATTG CAGATGATGA AAGTGATGAT GATGACGATG ATGATATTCC TCGAGAGGAT
    CATTATGCTC TTTTGGTAAA AGCATGGGAG ACCAAAGTTT TTCCAACCAT TCGAAGACGT
    TTCCGTAACG AGGCAGAGAG AAAGTCTGGC CTGGACCAGA TTAAGGGAGC TCTTCAGCTA
    GGAATGGTGG ATATAGCGAG GCAAACTGTA GAGTTCCTCT ATGAAGAAAA TGGTGGCATT
    CCAAGAGATC TCTATCTTCC TACTATTGAG GATATCAAGG ATGAGGCAAA TAAATTCACC
    ATTGATAAAG TTCGCAAAGG ACTCACAGTG GTCATTCGAT CCCCAGATAG CAACAATGTA
    GCTGGGACAA CTGTGGGAAC AGCTTTACCC AAGTTTGCCA TCCGTGGTAT GCTGAAGACA
    TTCTCTCTAC ATGGGGTGGT TCTGGATGTG GATTCAGTTA ATGAGCTGGT TCAGGTAGAG
    ACTTACCTCC GGAGTGAAGG GGTATTAGTG AGATACTGGT ATCCCATAGA TATGCTAGAA
    AGACCTCCTG CGGGATACAG GAGAATGGCA ACCAATGGAC TGGTGACTTT AGATAGCACT
    AACATCCAGA TTCATAGAGA GCTGTTGCGG TGTGAGTCTG CACTTGCCAG GCTTTACTGT
    CGTGTGGCTT TGCTTAACAT CTTTGCTCCT AAGTCTCCGC ACACTTTCAC TCGCCTGTTC
    CATATTCCTG CTATCCGAGA CATTACATTG GAGCATTTAC AGCTTCTGTC AAATCAGCTT
    CTAATGCCAC CCCTTCCAGA CGGAACCATT ACCTCCAGCT CTATCCTTCT TGCCCAGTCC
    TTGCAACATT GCATCTACTC CCAGAACTGC TCAACAACAG ATTTGTTCTA CCAGGGCAAT
    GCACAGCCAA TAAGAGAGTG GCTTGGTGTG GCCATAACCA GGGCCCTGCA TCAAGGAGAG
    GACAGTCTCT TGGATCTGAC AAAACAAATC TGTTCCTTCT TGCAAAATGC GCCGGAACAG
    TTTCCATCTG AAGAATTTCC CATCTCTGAA TCCAAGCTCA GTATGGATGT TAATTTTCCT
    GGGGCAGCTT TTGTTATTGT TTCTTGTATA GAGAGTCCGT CTGGATTTAG AAAAGACTCC
    TCCTTGTACA AGGCTCCCTG GTCTAGAGTC ATGGTATATG GCCTTGGACA TAAAGTAAAG
    AGGAATGGAC AGCTGAACCT GATAGAATCA GTTTGTTATC CACGTGATGC CTCCCCAAGT
    AACACAGGCC TCACTCCCCC ACCAACCACA AACCAGTACC CAACAGCGAT TATTCCCACT
    GACAAGGTCC ACGTCAAACT TGGTGTATCA CCTACTCCTG GTGCTGTGCT GGTCATCCAT
    TCCCTGCCTC TAGAGTTTCC GTTGGCAATG GCATTCACAG AACAGCTGTT AACCTGGAAG
    TTGGAGGACA CAGAAGAGAA GTCAGAAGAT GAGCTTGATA CTGTTCCATC CTCTGCTCTG
    TTGCAAGTGG TAGAACTTTT AGGTACTTTG ATTTGGACAA CAGACCTTGC TGCAGTAATT
    AAGGAGCTTC TGTTCCATCT GTTGGCTGAA CTATTAAGGA AAGTTCACCA TCTAGAGCAG
    AAGAAGAACC CAAGTGGATT ATCTTCCTCG ATAGCCCTGC AGTTGAATCC ATGTCTAGCA
    ATGCTTATGG CTTTACAGAC AGAACTGCGC AAGCTGTATG ATGAAGAAAC TCAAAACTGG
    GCATCTGGGA ATGCATGTGG GGGGTCTGTA CCTGGAGCCT CTGAGCAGGG AAGATTTTCA
    ACTTACTTTC ATGCCCTCAT GGAAGTGTGC CTGGCAGTGG CAGAAGTGAC GCTCCCAATC
    AACATGAGCT CAGCAATCAG TGTGATGGCA TCTACAAATG CCACTAATCT GAGTGACTCT
    TCTTCTTCCT CTTCCTCTTC CCCTGGACAG ACTCCTCAAA GTCCAAGTCT GTTATCAAAG
    AGAAAGAAAG TTAAGATCAA ACGTGAGAAG ACTGCATCTG GAAAACGTAC ACCTTCACGT
    GGTGCAGAGA GTGATGCTGC TTTGCTTAGC ATTGGAGGAA GCAAACCTGA GGACATGTTG
    TGGTTCCATA GGGCATTGAC TTTATTGATG ATCCTAAGGC ATCTAACTAA CAAGGATCCA
    CAGGGGATGG GTGTCACAAA CGATGCTATT GCTGATGCTA GCCAGGCTTT AGTTGGTCCC
    ACAGCTCACA GTCGTCTCCT TGTGATTTCT GGCATCCCTA CACACCTAGA AGAATCAGTG
    GTCAGAAGTG CAATTAGAAA GGCATGTAAT GCTCATGGTG GAGTCTTTAA AGATGAAATC
    TACATTCCAT TGCAGGAAAC TGAAACCCCT AAACCCAAAG ACAGAATAGA AGGGGCAGAA
    TGCAAGACAG AAATAGAAAA ACCCTCTACG CAGCATACAA CCGACAATAT GGATATCAGC
    AGCTCGAGCA GTGTAACTCC TGCAGCAAGT GTCACGGCAT CAGCTTCTAC AAGCCAAGCT
    TCATTGTGCA GCTCCCAAGG GGGGGTATCA CGGACCACAA GTGAAACATC TATGGAGCAG
    TTTGCCCCAG AACATGCTGT GCCATCAGGG CTTCTGGAAC CAACTATTAT CTCCAGCCAG
    GAGAGTCTGG ATCTTTCTAT GTGCAGCACA GGAAGCCTCG GCAGTCTGGG AAGTCTTGTT
    GAACAGCTTG ATAATGTTGA AAACTCTTTG TCTGACGTGG GCTCCCTATA CACAGCTACA
    TCACTAGATA GCCATATGGC AGAGTCGCGG CCAATCAAAG GATTTGCAGT TGTAGAGGTG
    CGATCACGAG CTAAAATAGA GAAAATCCGT GCCACTTTAT TTAACAGTAG TGACCTCATT
    GGGTTATCTG GTTTGGATGG TGAAGATGAG CTGATGGAAA TGTCTACTGA GGAGATCTTG
    ACTGTTTCAA CAGTGAATCA GAGCTTGTTT GATACCCAAG GAATACCCGG TCTGGCTGAT
    TATTTCATGG ATAAATCAAT GAAAGGTGAT AAATTAGTCC CGGAAGCTCG TGATGTTCTG
    ACTGAGATTT TCAAGAGCTG CATTCACGCC GAGCAGATGC TGAGTCTGAC TCCAGCCAAG
    TTTATCAAAG TGTCTGATAT CTACCTTAGC AAGGAGCAAA TCAATTCACA GACCCCAGGG
    AACTTACTGC ATACCTTTTT CACCAATGTT CGACCACCTA AAAAAGTCCT GGAAGATCAA
    CTAACGCAGA TCTTAAGAAA ATATGGAATT CCCAAGCCTA AATTTGACAA AAGCAAATAC
    AGCAAAACTG GGAAGGAGCA ACATCCTGTG AAAGTTGTAA GCACCAAAAG ACCTGTGGGC
    AAACCACCGA CCAAAGACAA GGCTGTATTC AACAGCGTCA GGACTGCTTT AAGTGAGAAA
    AAACCAACTG TGAAGCCAAA ATCTCCGGAG AAGGGCAGGC CAGATGAGAA GGACCCAGAC
    AAATCTCCAA CAAAGAAGCA AGAAATTCCC GAGGAGAGAT ATCTGACGTT GGATGGATTT
    CACCGCTTTG TAGTAGATCG GGCTAAGCAA GACATTCGAA GCATCTGGAG AGCTGTCTTG
    GCGTGTGGAT ATGATCTTCA TTTTGAAAGG TGTGCCTGCA TTGATACAAG GCATGCTCAG
    AAATCTAGCA GAAAATGGAC CTTGGAAATG GATGTTGCTC TTGTTCAGTA TATCAACCGC
    CTTTGCCGTC ACCTTGCCAT AACGCCAGCC CGACTACACC CTCATGAGGT TTATCTGGAT
    CTTGCAGATA CGGCTGACCC AAGAGTCTCC TGCCTGCTGA ATGTACCAAT AGAAAGCCTG
    CGCCTCCGAT TTGCTCTTCT TCAGTCCCTC AACACTACTT TAGAGACCTT TTTCCTGCCC
    TTAGTGGAGC TACGGCAAAC AGACGTATAC ACACACAGCA TCGCATCCCT ACTCCAGCTA
    GCTAAAGGGT TAATCTTTTA CGATACAAAA GTGAATGTGA TGAACCGGGT ATTGAACGCA
    ACAGTGCAGA GGACAGCAGA TCATGCAGCC CCTGAGATCA CGCTGGACCC ACTAGAAATT
    GTAGGAGGGG AAATTCGTAC CTCAGAAAAC TCCTACTTCT GCCAGGCAGC CCGACAGTTG
    GCATGCATCC CTTCCTCACA ACTGTGTGTC AAACTGGCAA GTGGAGGAGA CCCCACGTAT
    GCATTTAACA TCCGCTTTAC TGGAGAGGAG GTGCATGGTA CAAGTGGCTC ATTCCGTCAT
    TTCCTCTGGC AAGTGTGTAA AGAACTACAG AGCTCCTCGC TGTCTCTTCT ACTGCTCTGC
    CCCAGCTCTG CAGTTAATAA GAATAAGGGA AAATATCTTC TAACTCCCAG TCCCATAACA
    TATGCAGAAG AGCAGTTGCT CCATTTCTTT GGCCAACTAC TGGGAATAGC CATCAGAGCC
    GATGTTCCAC TCCCTCTGGA TCTACTCCCC TGCTTCTGGA AGATGCTTGT AGGAGAACCT
    TTGGACCCAG AAACTGATCT TTATGAAGCA GATATTCTCA CCCACAACTA CATCAAGAAG
    TTTGAAAATG TAAATGATGA GTCAGAGCTG GAAGCTCTTT GTGCTGAAAT TGCCTCTCAG
    CATCTTCCTA CCGAAAGTCC AGATTCTCCT CATAAACCAG CCTGCAAATT TACATACTTG
    ACAATGACTG GTGAAGAAGT GGAGCTGTGC CCTAAGGGAA GACACATTTC TGTAGTCTGG
    GAAAACAAAG ATATATATGC AATGGGAATC CGGAACCTTC GTATACGTGA GCTGAAGAAC
    CAGGAGTGTA TGAGCGCAGT AAGGGCTGGG CTGGGCTCCA TTATACCTCT GCAGCTCCTC
    ACCACTCTAA ATGCTCTAGA GATGGAACTA AGGACATGTG GCCTACCATA TATTAATTTG
    GAATTCTTAA AGGCTCACAC CATGTATCAA GTGGGTCTAA TGGAGACAGA TCAACACATT
    GAGTATTTTT GGTCTGCCTT AGAAATGTTC ACTCAGGAAG AACTTTGCAA ATTCATAAAA
    TTCGCCTGCA ATCAGGAACG CATTCCCTTT ACATGTCCAT GCAAAGATGG TGGGCCAGAC
    ACGGCTCATG TTCCACCTTA TCCTATGAAA ATAGCACCAC CCGATGGAGC AACAGGCTCC
    CCAGATTCCA GGTACATCCG AGTGGAGACA TGTATGTTCA TGATCAAGCT TCCGCAGTAC
    TCGTCTCTAG AGATGATGCT GGAGAAACTG AGATATGCCA TTCACTACAG GGAAGACCCA
    CTCAGCGGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.