e2f8 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	e2f8
Entrez Gene ID	100142691
Full Name	E2F transcription factor 8
General protein information	Preferred Names E2F transcription factor 8 Names transcription factor E2F8
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002942835.5	XP_002942881.1	transcription factor E2F8
XM_004913337.3	XP_004913394.1	transcription factor E2F8
XM_018090118.1	XP_017945607.1	transcription factor E2F8
XM_002942835.4	XP_002942881.1	transcription factor E2F8
XM_004913337.4	XP_004913394.1	transcription factor E2F8

Pathways from BioSystems

Homologies

Homo sapiens (human)	E2F8	NP_001243300.1
	E2F8	XP_001095088.1
Bos taurus (cattle)	E2F8	NP_001178163.1
Pan troglodytes (chimpanzee)	E2F8	XP_508325.2
Gallus gallus (chicken)	E2F8	XP_420910.4
Mus musculus (house mouse)	E2f8	NP_001013386.2
Canis lupus familiaris (dog)	E2F8	XP_534087.2
Rattus norvegicus (Norway rat)	E2f8	XP_006229325.1
Danio rerio (zebrafish)	e2f8	XP_005168886.1
Xenopus tropicalis (tropical clawed frog)	e2f8	XP_002942881.1

Gene Ontology

The following e2f8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the e2f8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa08473	XM_018090118.1 Latest version!	Xenopus tropicalis E2F transcription factor 8 (e2f8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa08473	XM_002942835.5 Latest version!	Xenopus tropicalis E2F transcription factor 8 (e2f8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa08473	XM_004913337.4 Latest version!	Xenopus tropicalis E2F transcription factor 8 (e2f8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OXa08473	XM_002942835.4	Xenopus tropicalis E2F transcription factor 8 (e2f8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa08473	XM_004913337.3	Xenopus tropicalis E2F transcription factor 8 (e2f8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

e2f8 ( XM_004913337.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913394.1 Xenopus tropicalis E2F transcription factor 8 (e2f8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa08473
Clone ID Related Accession (Same CDS sequence)	XM_002942835.5 , XM_004913337.3 , XM_018090118.1 , XM_002942835.4 , XM_004913337.4
Accession Version	XM_004913337.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transcription factor E2F8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683577.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004913337.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG GATCTAAGGA AAACTGTGGG TTCAATGGAA GTCCCATGGG CAGCAGGTCC 
CCCCCAAAGC AGCTCACATC TGCAGCCTCA GTGTTGGGGG AGATCCAGAT TGCAGCCGCC 
AATTTAAAGA CCCCTACAAA GCCTCAGGAA CGGAACAATG CCGACCCCTG GACCCCAACG 
GCCAATCTCA AAATGCTGAT CAGCGCCGCC AGCCCCGAGA TCCGGAACCG GGAGCGAGAG 
ATCTTGGAGG AGCAGTTTTC TGGAGATGAA CTGGAAAAGA CGCTGCCGAG CCGGAAGGAG 
AAGAGTTTGG GGTTACTGTG CCATAAGTTC CTGGCACGCT ACCCCAGTTA CCCCAATCCT 
GCTGTGAATA ACAGTATCTG CCTGGATGAA GTTGCTGGGG AGCTGAGCGT CGAGCGCAGG 
CGCATCTACG ACATCGTCAA CGTGCTGGAG AGTCTGCATA TGGTCAGCCG CTTGGCCAAA 
AACAAGTACA TCTGGCACGG CCGGCTCAAT CTGAGCAAAA CGTTCGACGC GCTGAAGAAG 
GTCGGCGAGG AGAACCGATA CGGGGAGCAG ATCCAGCTGC TGCGGAAGCG AGAGCAGGAA 
GAATGCGATT CCCAGAATTC CCCCAATGCG GAAACGCAAA AGCCTCTTGC TAAGCAGCCG 
GAAGTCGGGT TTGTGGAGCT GCCCGGATTG GAGTTCCGAG CTGCGTCGGT GAATAGCAGG 
AAAGAGAAGT CCCTGCGAGT AATGAGCCAG CGCTTCGTGA TGCTGTTCCT TGTGTCGGAC 
CCTCAGATCG TTAGCCTGGA AGTTGCTGCT AAAATATTAA TTGGGGAGGA CCAACTGGAG 
GATTTGGACA AAAGCAAATT TAAAACGAAG ATCCGGCGCC TGTACGATAT CGCCAATGTC 
CTGACCAGCC TCAATCTCAT AAAGAAAGTC CACGTGACGG AGGAGAAGGG GAGGAAGCCG 
GCTTTCCAGT GGACGTGCCC GGAGTTGTGT ACAGACGACC AAGAGAACAG ATCCAGCCCC 
GCTGCACTTA CCCCCGTGGC CATAGACCTG AGCTCCCCAA AGGAGAACTG TGCCAAAAAC 
CTCTTTGCCT CCGGAGGCAA AACCTTTACC CGCCACCCGT CTCTGATAAA ACTCGCCAAA 
AGCATCGAGA ACGACCGCAG GAAGATCAAC TCGGCGCCCT CCAGCCCCAT AAAGAGCGGA 
GACGGGTCCT CCAGCGCAGC CTCCAAAATG GCCCAACTTG CAGCGATTTG CAAACAGCAG 
CTCCAGCAGT CCAGGGATCA GACTAAGGTG AAGCTGAAGG TCTCCGCTTG CAAAGCCAAA 
TCCACCGTGA AACAGCCGGG GGGCTCCGAT AAGAACCAGA CCCCCACCTA CTGCCGAGCC 
ATCCCTCTGC TCCATCCTCA CCCCAGTGCT GCTCCCCCCT ACACTGTCAT TGTGCAGCCC 
CCCCAGGAAC AGACTCTCTC CAGGCAGAGC CCCCCAGCCC TGGCACATTC TGTTCCTCTA 
GCTCAGGGCT ACACGAATAG AACCCCCCCA GAGGCGCCTT TACAGGGGGG GAGACACGAG 
GGAGACGGCA CGTCGCACAG CGAGGATCAT TCGGCCCAGG AGCGGCACCC CAAACGGCTC 
CCCGAGTCCG ACCGCGGCTG CACCTCGAAA AGGATGAAAA GTTCTGCGGT AGATGATGTG 
ACAGAGACTC TCTATCCGTC TGGTTATCTA ATTCCCATAC ATTTGGCCCC AGTGGCCCCT 
GAACCCAGTA AAGAGAACAC GGGCCCCTCC TCGGAGAACA AGCTCTTTAC CTCCCCAATT 
CCAGGAGTCT TCCCTCTCAA GCTTATGTTC TCTCCCGGCC CGGTGACGGC GGTTCCGGTT 
ATGAGCCGCG GCGGGCAGCA CGTTGGAGGT GGGTCGGGTT CTGCCTCTAG GAGCCCCAGC 
CCGGGAATGT TCACTTTCGC CCTACAGAAC AGAGAGCTGA TCTCGGCAGG ACTCCCCCAA 
GGGGCTACCG TGTCACCGCG CAATGGGAGG GGCCAAGAGG AGCTGTCTGC GGCTTCAGTG 
TTAAACTGTA AACATGTGTC CCCAGTGCCT TATCACGGGC AGCCATTCAC CGTATTTGCC 
TTGCAGCAGT CGGCCGTTCC TGTGACCCCC AAAGGATATC ACTCCCTGCA GGAAACGTTC 
TTCCGTACCC CGGGGGGCAT GGGCTGCTCC CCCCCAGAAT CTGCACGGAA GCTGGATGTC 
GGCACAGACG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913394.1
CDS	170..2404
Translation	MEEGSKENCGFNGSPMGSRSPPKQLTSAASVLGEIQIAAANLKTPTKPQERNNADPWTPTANLKMLISAASPEIRNREREILEEQFSGDELEKTLPSRKEKSLGLLCHKFLARYPSYPNPAVNNSICLDEVAGELSVERRRIYDIVNVLESLHMVSRLAKNKYIWHGRLNLSKTFDALKKVGEENRYGEQIQLLRKREQEECDSQNSPNAETQKPLAKQPEVGFVELPGLEFRAASVNSRKEKSLRVMSQRFVMLFLVSDPQIVSLEVAAKILIGEDQLEDLDKSKFKTKIRRLYDIANVLTSLNLIKKVHVTEEKGRKPAFQWTCPELCTDDQENRSSPAALTPVAIDLSSPKENCAKNLFASGGKTFTRHPSLIKLAKSIENDRRKINSAPSSPIKSGDGSSSAASKMAQLAAICKQQLQQSRDQTKVKLKVSACKAKSTVKQPGGSDKNQTPTYCRAIPLLHPHPSAAPPYTVIVQPPQEQTLSRQSPPALAHSVPLAQGYTNRTPPEAPLQGGRHEGDGTSHSEDHSAQERHPKRLPESDRGCTSKRMKSSAVDDVTETLYPSGYLIPIHLAPVAPEPSKENTGPSSENKLFTSPIPGVFPLKLMFSPGPVTAVPVMSRGGQHVGGGSGSASRSPSPGMFTFALQNRELISAGLPQGATVSPRNGRGQEELSAASVLNCKHVSPVPYHGQPFTVFALQQSAVPVTPKGYHSLQETFFRTPGGMGCSPPESARKLDVGTDD

Target ORF information:

RefSeq Version	XM_004913337.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis E2F transcription factor 8 (e2f8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913337.3

ORF Insert Sequence:

ATGGAGGAGG GATCTAAGGA AAACTGTGGG TTCAATGGAA GTCCCATGGG CAGCAGGTCC 
CCCCCAAAGC AGCTCACATC TGCAGCCTCA GTGTTGGGGG AGATCCAGAT TGCAGCCGCC 
AATTTAAAGA CCCCTACAAA GCCTCAGGAA CGGAACAATG CCGACCCCTG GACCCCAACG 
GCCAATCTCA AAATGCTGAT CAGCGCCGCC AGCCCCGAGA TCCGGAACCG GGAGCGAGAG 
ATCTTGGAGG AGCAGTTTTC TGGAGATGAA CTGGAAAAGA CGCTGCCGAG CCGGAAGGAG 
AAGAGTTTGG GGTTACTGTG CCATAAGTTC CTGGCACGCT ACCCCAGTTA CCCCAATCCT 
GCTGTGAATA ACAGTATCTG CCTGGATGAA GTTGCTGGGG AGCTGAGCGT CGAGCGCAGG 
CGCATCTACG ACATCGTCAA CGTGCTGGAG AGTCTGCATA TGGTCAGCCG CTTGGCCAAA 
AACAAGTACA TCTGGCACGG CCGGCTCAAT CTGAGCAAAA CGTTCGACGC GCTGAAGAAG 
GTCGGCGAGG AGAACCGATA CGGGGAGCAG ATCCAGCTGC TGCGGAAGCG AGAGCAGGAA 
GAATGCGATT CCCAGAATTC CCCCAATGCG GAAACGCAAA AGCCTCTTGC TAAGCAGCCG 
GAAGTCGGGT TTGTGGAGCT GCCCGGATTG GAGTTCCGAG CTGCGTCGGT GAATAGCAGG 
AAAGAGAAGT CCCTGCGAGT AATGAGCCAG CGCTTCGTGA TGCTGTTCCT TGTGTCGGAC 
CCTCAGATCG TTAGCCTGGA AGTTGCTGCT AAAATATTAA TTGGGGAGGA CCAACTGGAG 
GATTTGGACA AAAGCAAATT TAAAACGAAG ATCCGGCGCC TGTACGATAT CGCCAATGTC 
CTGACCAGCC TCAATCTCAT AAAGAAAGTC CACGTGACGG AGGAGAAGGG GAGGAAGCCG 
GCTTTCCAGT GGACGTGCCC GGAGTTGTGT ACAGACGACC AAGAGAACAG ATCCAGCCCC 
GCTGCACTTA CCCCCGTGGC CATAGACCTG AGCTCCCCAA AGGAGAACTG TGCCAAAAAC 
CTCTTTGCCT CCGGAGGCAA AACCTTTACC CGCCACCCGT CTCTGATAAA ACTCGCCAAA 
AGCATCGAGA ACGACCGCAG GAAGATCAAC TCGGCGCCCT CCAGCCCCAT AAAGAGCGGA 
GACGGGTCCT CCAGCGCAGC CTCCAAAATG GCCCAACTTG CAGCGATTTG CAAACAGCAG 
CTCCAGCAGT CCAGGGATCA GACTAAGGTG AAGCTGAAGG TCTCCGCTTG CAAAGCCAAA 
TCCACCGTGA AACAGCCGGG GGGCTCCGAT AAGAACCAGA CCCCCACCTA CTGCCGAGCC 
ATCCCTCTGC TCCATCCTCA CCCCAGTGCT GCTCCCCCCT ACACTGTCAT TGTGCAGCCC 
CCCCAGGAAC AGACTCTCTC CAGGCAGAGC CCCCCAGCCC TGGCACATTC TGTTCCTCTA 
GCTCAGGGCT ACACGAATAG AACCCCCCCA GAGGCGCCTT TACAGGGGGG GAGACACGAG 
GGAGACGGCA CGTCGCACAG CGAGGATCAT TCGGCCCAGG AGCGGCACCC CAAACGGCTC 
CCCGAGTCCG ACCGCGGCTG CACCTCGAAA AGGATGAAAA GTTCTGCGGT AGATGATGTG 
ACAGAGACTC TCTATCCGTC TGGTTATCTA ATTCCCATAC ATTTGGCCCC AGTGGCCCCT 
GAACCCAGTA AAGAGAACAC GGGCCCCTCC TCGGAGAACA AGCTCTTTAC CTCCCCAATT 
CCAGGAGTCT TCCCTCTCAA GCTTATGTTC TCTCCCGGCC CGGTGACGGC GGTTCCGGTT 
ATGAGCCGCG GCGGGCAGCA CGTTGGAGGT GGGTCGGGTT CTGCCTCTAG GAGCCCCAGC 
CCGGGAATGT TCACTTTCGC CCTACAGAAC AGAGAGCTGA TCTCGGCAGG ACTCCCCCAA 
GGGGCTACCG TGTCACCGCG CAATGGGAGG GGCCAAGAGG AGCTGTCTGC GGCTTCAGTG 
TTAAACTGTA AACATGTGTC CCCAGTGCCT TATCACGGGC AGCCATTCAC CGTATTTGCC 
TTGCAGCAGT CGGCCGTTCC TGTGACCCCC AAAGGATATC ACTCCCTGCA GGAAACGTTC 
TTCCGTACCC CGGGGGGCAT GGGCTGCTCC CCCCCAGAAT CTGCACGGAA GCTGGATGTC 
GGCACAGACG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

e2f8 ( XM_018090118.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017945607.1 Xenopus tropicalis E2F transcription factor 8 (e2f8), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa08473
Clone ID Related Accession (Same CDS sequence)	XM_002942835.5 , XM_004913337.3 , XM_018090118.1 , XM_002942835.4 , XM_004913337.4
Accession Version	XM_018090118.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transcription factor E2F8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683577.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG GATCTAAGGA AAACTGTGGG TTCAATGGAA GTCCCATGGG CAGCAGGTCC 
CCCCCAAAGC AGCTCACATC TGCAGCCTCA GTGTTGGGGG AGATCCAGAT TGCAGCCGCC 
AATTTAAAGA CCCCTACAAA GCCTCAGGAA CGGAACAATG CCGACCCCTG GACCCCAACG 
GCCAATCTCA AAATGCTGAT CAGCGCCGCC AGCCCCGAGA TCCGGAACCG GGAGCGAGAG 
ATCTTGGAGG AGCAGTTTTC TGGAGATGAA CTGGAAAAGA CGCTGCCGAG CCGGAAGGAG 
AAGAGTTTGG GGTTACTGTG CCATAAGTTC CTGGCACGCT ACCCCAGTTA CCCCAATCCT 
GCTGTGAATA ACAGTATCTG CCTGGATGAA GTTGCTGGGG AGCTGAGCGT CGAGCGCAGG 
CGCATCTACG ACATCGTCAA CGTGCTGGAG AGTCTGCATA TGGTCAGCCG CTTGGCCAAA 
AACAAGTACA TCTGGCACGG CCGGCTCAAT CTGAGCAAAA CGTTCGACGC GCTGAAGAAG 
GTCGGCGAGG AGAACCGATA CGGGGAGCAG ATCCAGCTGC TGCGGAAGCG AGAGCAGGAA 
GAATGCGATT CCCAGAATTC CCCCAATGCG GAAACGCAAA AGCCTCTTGC TAAGCAGCCG 
GAAGTCGGGT TTGTGGAGCT GCCCGGATTG GAGTTCCGAG CTGCGTCGGT GAATAGCAGG 
AAAGAGAAGT CCCTGCGAGT AATGAGCCAG CGCTTCGTGA TGCTGTTCCT TGTGTCGGAC 
CCTCAGATCG TTAGCCTGGA AGTTGCTGCT AAAATATTAA TTGGGGAGGA CCAACTGGAG 
GATTTGGACA AAAGCAAATT TAAAACGAAG ATCCGGCGCC TGTACGATAT CGCCAATGTC 
CTGACCAGCC TCAATCTCAT AAAGAAAGTC CACGTGACGG AGGAGAAGGG GAGGAAGCCG 
GCTTTCCAGT GGACGTGCCC GGAGTTGTGT ACAGACGACC AAGAGAACAG ATCCAGCCCC 
GCTGCACTTA CCCCCGTGGC CATAGACCTG AGCTCCCCAA AGGAGAACTG TGCCAAAAAC 
CTCTTTGCCT CCGGAGGCAA AACCTTTACC CGCCACCCGT CTCTGATAAA ACTCGCCAAA 
AGCATCGAGA ACGACCGCAG GAAGATCAAC TCGGCGCCCT CCAGCCCCAT AAAGAGCGGA 
GACGGGTCCT CCAGCGCAGC CTCCAAAATG GCCCAACTTG CAGCGATTTG CAAACAGCAG 
CTCCAGCAGT CCAGGGATCA GACTAAGGTG AAGCTGAAGG TCTCCGCTTG CAAAGCCAAA 
TCCACCGTGA AACAGCCGGG GGGCTCCGAT AAGAACCAGA CCCCCACCTA CTGCCGAGCC 
ATCCCTCTGC TCCATCCTCA CCCCAGTGCT GCTCCCCCCT ACACTGTCAT TGTGCAGCCC 
CCCCAGGAAC AGACTCTCTC CAGGCAGAGC CCCCCAGCCC TGGCACATTC TGTTCCTCTA 
GCTCAGGGCT ACACGAATAG AACCCCCCCA GAGGCGCCTT TACAGGGGGG GAGACACGAG 
GGAGACGGCA CGTCGCACAG CGAGGATCAT TCGGCCCAGG AGCGGCACCC CAAACGGCTC 
CCCGAGTCCG ACCGCGGCTG CACCTCGAAA AGGATGAAAA GTTCTGCGGT AGATGATGTG 
ACAGAGACTC TCTATCCGTC TGGTTATCTA ATTCCCATAC ATTTGGCCCC AGTGGCCCCT 
GAACCCAGTA AAGAGAACAC GGGCCCCTCC TCGGAGAACA AGCTCTTTAC CTCCCCAATT 
CCAGGAGTCT TCCCTCTCAA GCTTATGTTC TCTCCCGGCC CGGTGACGGC GGTTCCGGTT 
ATGAGCCGCG GCGGGCAGCA CGTTGGAGGT GGGTCGGGTT CTGCCTCTAG GAGCCCCAGC 
CCGGGAATGT TCACTTTCGC CCTACAGAAC AGAGAGCTGA TCTCGGCAGG ACTCCCCCAA 
GGGGCTACCG TGTCACCGCG CAATGGGAGG GGCCAAGAGG AGCTGTCTGC GGCTTCAGTG 
TTAAACTGTA AACATGTGTC CCCAGTGCCT TATCACGGGC AGCCATTCAC CGTATTTGCC 
TTGCAGCAGT CGGCCGTTCC TGTGACCCCC AAAGGATATC ACTCCCTGCA GGAAACGTTC 
TTCCGTACCC CGGGGGGCAT GGGCTGCTCC CCCCCAGAAT CTGCACGGAA GCTGGATGTC 
GGCACAGACG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017945607.1
CDS	87..2321
Translation	MEEGSKENCGFNGSPMGSRSPPKQLTSAASVLGEIQIAAANLKTPTKPQERNNADPWTPTANLKMLISAASPEIRNREREILEEQFSGDELEKTLPSRKEKSLGLLCHKFLARYPSYPNPAVNNSICLDEVAGELSVERRRIYDIVNVLESLHMVSRLAKNKYIWHGRLNLSKTFDALKKVGEENRYGEQIQLLRKREQEECDSQNSPNAETQKPLAKQPEVGFVELPGLEFRAASVNSRKEKSLRVMSQRFVMLFLVSDPQIVSLEVAAKILIGEDQLEDLDKSKFKTKIRRLYDIANVLTSLNLIKKVHVTEEKGRKPAFQWTCPELCTDDQENRSSPAALTPVAIDLSSPKENCAKNLFASGGKTFTRHPSLIKLAKSIENDRRKINSAPSSPIKSGDGSSSAASKMAQLAAICKQQLQQSRDQTKVKLKVSACKAKSTVKQPGGSDKNQTPTYCRAIPLLHPHPSAAPPYTVIVQPPQEQTLSRQSPPALAHSVPLAQGYTNRTPPEAPLQGGRHEGDGTSHSEDHSAQERHPKRLPESDRGCTSKRMKSSAVDDVTETLYPSGYLIPIHLAPVAPEPSKENTGPSSENKLFTSPIPGVFPLKLMFSPGPVTAVPVMSRGGQHVGGGSGSASRSPSPGMFTFALQNRELISAGLPQGATVSPRNGRGQEELSAASVLNCKHVSPVPYHGQPFTVFALQQSAVPVTPKGYHSLQETFFRTPGGMGCSPPESARKLDVGTDD

Target ORF information:

RefSeq Version	XM_018090118.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis E2F transcription factor 8 (e2f8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018090118.1

ORF Insert Sequence:

ATGGAGGAGG GATCTAAGGA AAACTGTGGG TTCAATGGAA GTCCCATGGG CAGCAGGTCC 
CCCCCAAAGC AGCTCACATC TGCAGCCTCA GTGTTGGGGG AGATCCAGAT TGCAGCCGCC 
AATTTAAAGA CCCCTACAAA GCCTCAGGAA CGGAACAATG CCGACCCCTG GACCCCAACG 
GCCAATCTCA AAATGCTGAT CAGCGCCGCC AGCCCCGAGA TCCGGAACCG GGAGCGAGAG 
ATCTTGGAGG AGCAGTTTTC TGGAGATGAA CTGGAAAAGA CGCTGCCGAG CCGGAAGGAG 
AAGAGTTTGG GGTTACTGTG CCATAAGTTC CTGGCACGCT ACCCCAGTTA CCCCAATCCT 
GCTGTGAATA ACAGTATCTG CCTGGATGAA GTTGCTGGGG AGCTGAGCGT CGAGCGCAGG 
CGCATCTACG ACATCGTCAA CGTGCTGGAG AGTCTGCATA TGGTCAGCCG CTTGGCCAAA 
AACAAGTACA TCTGGCACGG CCGGCTCAAT CTGAGCAAAA CGTTCGACGC GCTGAAGAAG 
GTCGGCGAGG AGAACCGATA CGGGGAGCAG ATCCAGCTGC TGCGGAAGCG AGAGCAGGAA 
GAATGCGATT CCCAGAATTC CCCCAATGCG GAAACGCAAA AGCCTCTTGC TAAGCAGCCG 
GAAGTCGGGT TTGTGGAGCT GCCCGGATTG GAGTTCCGAG CTGCGTCGGT GAATAGCAGG 
AAAGAGAAGT CCCTGCGAGT AATGAGCCAG CGCTTCGTGA TGCTGTTCCT TGTGTCGGAC 
CCTCAGATCG TTAGCCTGGA AGTTGCTGCT AAAATATTAA TTGGGGAGGA CCAACTGGAG 
GATTTGGACA AAAGCAAATT TAAAACGAAG ATCCGGCGCC TGTACGATAT CGCCAATGTC 
CTGACCAGCC TCAATCTCAT AAAGAAAGTC CACGTGACGG AGGAGAAGGG GAGGAAGCCG 
GCTTTCCAGT GGACGTGCCC GGAGTTGTGT ACAGACGACC AAGAGAACAG ATCCAGCCCC 
GCTGCACTTA CCCCCGTGGC CATAGACCTG AGCTCCCCAA AGGAGAACTG TGCCAAAAAC 
CTCTTTGCCT CCGGAGGCAA AACCTTTACC CGCCACCCGT CTCTGATAAA ACTCGCCAAA 
AGCATCGAGA ACGACCGCAG GAAGATCAAC TCGGCGCCCT CCAGCCCCAT AAAGAGCGGA 
GACGGGTCCT CCAGCGCAGC CTCCAAAATG GCCCAACTTG CAGCGATTTG CAAACAGCAG 
CTCCAGCAGT CCAGGGATCA GACTAAGGTG AAGCTGAAGG TCTCCGCTTG CAAAGCCAAA 
TCCACCGTGA AACAGCCGGG GGGCTCCGAT AAGAACCAGA CCCCCACCTA CTGCCGAGCC 
ATCCCTCTGC TCCATCCTCA CCCCAGTGCT GCTCCCCCCT ACACTGTCAT TGTGCAGCCC 
CCCCAGGAAC AGACTCTCTC CAGGCAGAGC CCCCCAGCCC TGGCACATTC TGTTCCTCTA 
GCTCAGGGCT ACACGAATAG AACCCCCCCA GAGGCGCCTT TACAGGGGGG GAGACACGAG 
GGAGACGGCA CGTCGCACAG CGAGGATCAT TCGGCCCAGG AGCGGCACCC CAAACGGCTC 
CCCGAGTCCG ACCGCGGCTG CACCTCGAAA AGGATGAAAA GTTCTGCGGT AGATGATGTG 
ACAGAGACTC TCTATCCGTC TGGTTATCTA ATTCCCATAC ATTTGGCCCC AGTGGCCCCT 
GAACCCAGTA AAGAGAACAC GGGCCCCTCC TCGGAGAACA AGCTCTTTAC CTCCCCAATT 
CCAGGAGTCT TCCCTCTCAA GCTTATGTTC TCTCCCGGCC CGGTGACGGC GGTTCCGGTT 
ATGAGCCGCG GCGGGCAGCA CGTTGGAGGT GGGTCGGGTT CTGCCTCTAG GAGCCCCAGC 
CCGGGAATGT TCACTTTCGC CCTACAGAAC AGAGAGCTGA TCTCGGCAGG ACTCCCCCAA 
GGGGCTACCG TGTCACCGCG CAATGGGAGG GGCCAAGAGG AGCTGTCTGC GGCTTCAGTG 
TTAAACTGTA AACATGTGTC CCCAGTGCCT TATCACGGGC AGCCATTCAC CGTATTTGCC 
TTGCAGCAGT CGGCCGTTCC TGTGACCCCC AAAGGATATC ACTCCCTGCA GGAAACGTTC 
TTCCGTACCC CGGGGGGCAT GGGCTGCTCC CCCCCAGAAT CTGCACGGAA GCTGGATGTC 
GGCACAGACG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

e2f8 ( XM_002942835.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002942881.1 Xenopus tropicalis E2F transcription factor 8 (e2f8), transcript variant X3, mRNA.

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CloneID	OXa08473
Clone ID Related Accession (Same CDS sequence)	XM_002942835.5 , XM_004913337.3 , XM_018090118.1 , XM_002942835.4 , XM_004913337.4
Accession Version	XM_002942835.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transcription factor E2F8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683577.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002942835.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG GATCTAAGGA AAACTGTGGG TTCAATGGAA GTCCCATGGG CAGCAGGTCC 
CCCCCAAAGC AGCTCACATC TGCAGCCTCA GTGTTGGGGG AGATCCAGAT TGCAGCCGCC 
AATTTAAAGA CCCCTACAAA GCCTCAGGAA CGGAACAATG CCGACCCCTG GACCCCAACG 
GCCAATCTCA AAATGCTGAT CAGCGCCGCC AGCCCCGAGA TCCGGAACCG GGAGCGAGAG 
ATCTTGGAGG AGCAGTTTTC TGGAGATGAA CTGGAAAAGA CGCTGCCGAG CCGGAAGGAG 
AAGAGTTTGG GGTTACTGTG CCATAAGTTC CTGGCACGCT ACCCCAGTTA CCCCAATCCT 
GCTGTGAATA ACAGTATCTG CCTGGATGAA GTTGCTGGGG AGCTGAGCGT CGAGCGCAGG 
CGCATCTACG ACATCGTCAA CGTGCTGGAG AGTCTGCATA TGGTCAGCCG CTTGGCCAAA 
AACAAGTACA TCTGGCACGG CCGGCTCAAT CTGAGCAAAA CGTTCGACGC GCTGAAGAAG 
GTCGGCGAGG AGAACCGATA CGGGGAGCAG ATCCAGCTGC TGCGGAAGCG AGAGCAGGAA 
GAATGCGATT CCCAGAATTC CCCCAATGCG GAAACGCAAA AGCCTCTTGC TAAGCAGCCG 
GAAGTCGGGT TTGTGGAGCT GCCCGGATTG GAGTTCCGAG CTGCGTCGGT GAATAGCAGG 
AAAGAGAAGT CCCTGCGAGT AATGAGCCAG CGCTTCGTGA TGCTGTTCCT TGTGTCGGAC 
CCTCAGATCG TTAGCCTGGA AGTTGCTGCT AAAATATTAA TTGGGGAGGA CCAACTGGAG 
GATTTGGACA AAAGCAAATT TAAAACGAAG ATCCGGCGCC TGTACGATAT CGCCAATGTC 
CTGACCAGCC TCAATCTCAT AAAGAAAGTC CACGTGACGG AGGAGAAGGG GAGGAAGCCG 
GCTTTCCAGT GGACGTGCCC GGAGTTGTGT ACAGACGACC AAGAGAACAG ATCCAGCCCC 
GCTGCACTTA CCCCCGTGGC CATAGACCTG AGCTCCCCAA AGGAGAACTG TGCCAAAAAC 
CTCTTTGCCT CCGGAGGCAA AACCTTTACC CGCCACCCGT CTCTGATAAA ACTCGCCAAA 
AGCATCGAGA ACGACCGCAG GAAGATCAAC TCGGCGCCCT CCAGCCCCAT AAAGAGCGGA 
GACGGGTCCT CCAGCGCAGC CTCCAAAATG GCCCAACTTG CAGCGATTTG CAAACAGCAG 
CTCCAGCAGT CCAGGGATCA GACTAAGGTG AAGCTGAAGG TCTCCGCTTG CAAAGCCAAA 
TCCACCGTGA AACAGCCGGG GGGCTCCGAT AAGAACCAGA CCCCCACCTA CTGCCGAGCC 
ATCCCTCTGC TCCATCCTCA CCCCAGTGCT GCTCCCCCCT ACACTGTCAT TGTGCAGCCC 
CCCCAGGAAC AGACTCTCTC CAGGCAGAGC CCCCCAGCCC TGGCACATTC TGTTCCTCTA 
GCTCAGGGCT ACACGAATAG AACCCCCCCA GAGGCGCCTT TACAGGGGGG GAGACACGAG 
GGAGACGGCA CGTCGCACAG CGAGGATCAT TCGGCCCAGG AGCGGCACCC CAAACGGCTC 
CCCGAGTCCG ACCGCGGCTG CACCTCGAAA AGGATGAAAA GTTCTGCGGT AGATGATGTG 
ACAGAGACTC TCTATCCGTC TGGTTATCTA ATTCCCATAC ATTTGGCCCC AGTGGCCCCT 
GAACCCAGTA AAGAGAACAC GGGCCCCTCC TCGGAGAACA AGCTCTTTAC CTCCCCAATT 
CCAGGAGTCT TCCCTCTCAA GCTTATGTTC TCTCCCGGCC CGGTGACGGC GGTTCCGGTT 
ATGAGCCGCG GCGGGCAGCA CGTTGGAGGT GGGTCGGGTT CTGCCTCTAG GAGCCCCAGC 
CCGGGAATGT TCACTTTCGC CCTACAGAAC AGAGAGCTGA TCTCGGCAGG ACTCCCCCAA 
GGGGCTACCG TGTCACCGCG CAATGGGAGG GGCCAAGAGG AGCTGTCTGC GGCTTCAGTG 
TTAAACTGTA AACATGTGTC CCCAGTGCCT TATCACGGGC AGCCATTCAC CGTATTTGCC 
TTGCAGCAGT CGGCCGTTCC TGTGACCCCC AAAGGATATC ACTCCCTGCA GGAAACGTTC 
TTCCGTACCC CGGGGGGCAT GGGCTGCTCC CCCCCAGAAT CTGCACGGAA GCTGGATGTC 
GGCACAGACG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002942881.1
CDS	230..2464
Translation	MEEGSKENCGFNGSPMGSRSPPKQLTSAASVLGEIQIAAANLKTPTKPQERNNADPWTPTANLKMLISAASPEIRNREREILEEQFSGDELEKTLPSRKEKSLGLLCHKFLARYPSYPNPAVNNSICLDEVAGELSVERRRIYDIVNVLESLHMVSRLAKNKYIWHGRLNLSKTFDALKKVGEENRYGEQIQLLRKREQEECDSQNSPNAETQKPLAKQPEVGFVELPGLEFRAASVNSRKEKSLRVMSQRFVMLFLVSDPQIVSLEVAAKILIGEDQLEDLDKSKFKTKIRRLYDIANVLTSLNLIKKVHVTEEKGRKPAFQWTCPELCTDDQENRSSPAALTPVAIDLSSPKENCAKNLFASGGKTFTRHPSLIKLAKSIENDRRKINSAPSSPIKSGDGSSSAASKMAQLAAICKQQLQQSRDQTKVKLKVSACKAKSTVKQPGGSDKNQTPTYCRAIPLLHPHPSAAPPYTVIVQPPQEQTLSRQSPPALAHSVPLAQGYTNRTPPEAPLQGGRHEGDGTSHSEDHSAQERHPKRLPESDRGCTSKRMKSSAVDDVTETLYPSGYLIPIHLAPVAPEPSKENTGPSSENKLFTSPIPGVFPLKLMFSPGPVTAVPVMSRGGQHVGGGSGSASRSPSPGMFTFALQNRELISAGLPQGATVSPRNGRGQEELSAASVLNCKHVSPVPYHGQPFTVFALQQSAVPVTPKGYHSLQETFFRTPGGMGCSPPESARKLDVGTDD

Target ORF information:

RefSeq Version	XM_002942835.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis E2F transcription factor 8 (e2f8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002942835.4

ORF Insert Sequence:

ATGGAGGAGG GATCTAAGGA AAACTGTGGG TTCAATGGAA GTCCCATGGG CAGCAGGTCC 
CCCCCAAAGC AGCTCACATC TGCAGCCTCA GTGTTGGGGG AGATCCAGAT TGCAGCCGCC 
AATTTAAAGA CCCCTACAAA GCCTCAGGAA CGGAACAATG CCGACCCCTG GACCCCAACG 
GCCAATCTCA AAATGCTGAT CAGCGCCGCC AGCCCCGAGA TCCGGAACCG GGAGCGAGAG 
ATCTTGGAGG AGCAGTTTTC TGGAGATGAA CTGGAAAAGA CGCTGCCGAG CCGGAAGGAG 
AAGAGTTTGG GGTTACTGTG CCATAAGTTC CTGGCACGCT ACCCCAGTTA CCCCAATCCT 
GCTGTGAATA ACAGTATCTG CCTGGATGAA GTTGCTGGGG AGCTGAGCGT CGAGCGCAGG 
CGCATCTACG ACATCGTCAA CGTGCTGGAG AGTCTGCATA TGGTCAGCCG CTTGGCCAAA 
AACAAGTACA TCTGGCACGG CCGGCTCAAT CTGAGCAAAA CGTTCGACGC GCTGAAGAAG 
GTCGGCGAGG AGAACCGATA CGGGGAGCAG ATCCAGCTGC TGCGGAAGCG AGAGCAGGAA 
GAATGCGATT CCCAGAATTC CCCCAATGCG GAAACGCAAA AGCCTCTTGC TAAGCAGCCG 
GAAGTCGGGT TTGTGGAGCT GCCCGGATTG GAGTTCCGAG CTGCGTCGGT GAATAGCAGG 
AAAGAGAAGT CCCTGCGAGT AATGAGCCAG CGCTTCGTGA TGCTGTTCCT TGTGTCGGAC 
CCTCAGATCG TTAGCCTGGA AGTTGCTGCT AAAATATTAA TTGGGGAGGA CCAACTGGAG 
GATTTGGACA AAAGCAAATT TAAAACGAAG ATCCGGCGCC TGTACGATAT CGCCAATGTC 
CTGACCAGCC TCAATCTCAT AAAGAAAGTC CACGTGACGG AGGAGAAGGG GAGGAAGCCG 
GCTTTCCAGT GGACGTGCCC GGAGTTGTGT ACAGACGACC AAGAGAACAG ATCCAGCCCC 
GCTGCACTTA CCCCCGTGGC CATAGACCTG AGCTCCCCAA AGGAGAACTG TGCCAAAAAC 
CTCTTTGCCT CCGGAGGCAA AACCTTTACC CGCCACCCGT CTCTGATAAA ACTCGCCAAA 
AGCATCGAGA ACGACCGCAG GAAGATCAAC TCGGCGCCCT CCAGCCCCAT AAAGAGCGGA 
GACGGGTCCT CCAGCGCAGC CTCCAAAATG GCCCAACTTG CAGCGATTTG CAAACAGCAG 
CTCCAGCAGT CCAGGGATCA GACTAAGGTG AAGCTGAAGG TCTCCGCTTG CAAAGCCAAA 
TCCACCGTGA AACAGCCGGG GGGCTCCGAT AAGAACCAGA CCCCCACCTA CTGCCGAGCC 
ATCCCTCTGC TCCATCCTCA CCCCAGTGCT GCTCCCCCCT ACACTGTCAT TGTGCAGCCC 
CCCCAGGAAC AGACTCTCTC CAGGCAGAGC CCCCCAGCCC TGGCACATTC TGTTCCTCTA 
GCTCAGGGCT ACACGAATAG AACCCCCCCA GAGGCGCCTT TACAGGGGGG GAGACACGAG 
GGAGACGGCA CGTCGCACAG CGAGGATCAT TCGGCCCAGG AGCGGCACCC CAAACGGCTC 
CCCGAGTCCG ACCGCGGCTG CACCTCGAAA AGGATGAAAA GTTCTGCGGT AGATGATGTG 
ACAGAGACTC TCTATCCGTC TGGTTATCTA ATTCCCATAC ATTTGGCCCC AGTGGCCCCT 
GAACCCAGTA AAGAGAACAC GGGCCCCTCC TCGGAGAACA AGCTCTTTAC CTCCCCAATT 
CCAGGAGTCT TCCCTCTCAA GCTTATGTTC TCTCCCGGCC CGGTGACGGC GGTTCCGGTT 
ATGAGCCGCG GCGGGCAGCA CGTTGGAGGT GGGTCGGGTT CTGCCTCTAG GAGCCCCAGC 
CCGGGAATGT TCACTTTCGC CCTACAGAAC AGAGAGCTGA TCTCGGCAGG ACTCCCCCAA 
GGGGCTACCG TGTCACCGCG CAATGGGAGG GGCCAAGAGG AGCTGTCTGC GGCTTCAGTG 
TTAAACTGTA AACATGTGTC CCCAGTGCCT TATCACGGGC AGCCATTCAC CGTATTTGCC 
TTGCAGCAGT CGGCCGTTCC TGTGACCCCC AAAGGATATC ACTCCCTGCA GGAAACGTTC 
TTCCGTACCC CGGGGGGCAT GGGCTGCTCC CCCCCAGAAT CTGCACGGAA GCTGGATGTC 
GGCACAGACG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

e2f8 ( XM_002942835.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002942881.1 Xenopus tropicalis E2F transcription factor 8 (e2f8), transcript variant X2, mRNA.

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CloneID	OXa08473
Clone ID Related Accession (Same CDS sequence)	XM_002942835.5 , XM_004913337.3 , XM_018090118.1 , XM_002942835.4 , XM_004913337.4
Accession Version	XM_002942835.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transcription factor E2F8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002942835.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG GATCTAAGGA AAACTGTGGG TTCAATGGAA GTCCCATGGG CAGCAGGTCC 
CCCCCAAAGC AGCTCACATC TGCAGCCTCA GTGTTGGGGG AGATCCAGAT TGCAGCCGCC 
AATTTAAAGA CCCCTACAAA GCCTCAGGAA CGGAACAATG CCGACCCCTG GACCCCAACG 
GCCAATCTCA AAATGCTGAT CAGCGCCGCC AGCCCCGAGA TCCGGAACCG GGAGCGAGAG 
ATCTTGGAGG AGCAGTTTTC TGGAGATGAA CTGGAAAAGA CGCTGCCGAG CCGGAAGGAG 
AAGAGTTTGG GGTTACTGTG CCATAAGTTC CTGGCACGCT ACCCCAGTTA CCCCAATCCT 
GCTGTGAATA ACAGTATCTG CCTGGATGAA GTTGCTGGGG AGCTGAGCGT CGAGCGCAGG 
CGCATCTACG ACATCGTCAA CGTGCTGGAG AGTCTGCATA TGGTCAGCCG CTTGGCCAAA 
AACAAGTACA TCTGGCACGG CCGGCTCAAT CTGAGCAAAA CGTTCGACGC GCTGAAGAAG 
GTCGGCGAGG AGAACCGATA CGGGGAGCAG ATCCAGCTGC TGCGGAAGCG AGAGCAGGAA 
GAATGCGATT CCCAGAATTC CCCCAATGCG GAAACGCAAA AGCCTCTTGC TAAGCAGCCG 
GAAGTCGGGT TTGTGGAGCT GCCCGGATTG GAGTTCCGAG CTGCGTCGGT GAATAGCAGG 
AAAGAGAAGT CCCTGCGAGT AATGAGCCAG CGCTTCGTGA TGCTGTTCCT TGTGTCGGAC 
CCTCAGATCG TTAGCCTGGA AGTTGCTGCT AAAATATTAA TTGGGGAGGA CCAACTGGAG 
GATTTGGACA AAAGCAAATT TAAAACGAAG ATCCGGCGCC TGTACGATAT CGCCAATGTC 
CTGACCAGCC TCAATCTCAT AAAGAAAGTC CACGTGACGG AGGAGAAGGG GAGGAAGCCG 
GCTTTCCAGT GGACGTGCCC GGAGTTGTGT ACAGACGACC AAGAGAACAG ATCCAGCCCC 
GCTGCACTTA CCCCCGTGGC CATAGACCTG AGCTCCCCAA AGGAGAACTG TGCCAAAAAC 
CTCTTTGCCT CCGGAGGCAA AACCTTTACC CGCCACCCGT CTCTGATAAA ACTCGCCAAA 
AGCATCGAGA ACGACCGCAG GAAGATCAAC TCGGCGCCCT CCAGCCCCAT AAAGAGCGGA 
GACGGGTCCT CCAGCGCAGC CTCCAAAATG GCCCAACTTG CAGCGATTTG CAAACAGCAG 
CTCCAGCAGT CCAGGGATCA GACTAAGGTG AAGCTGAAGG TCTCCGCTTG CAAAGCCAAA 
TCCACCGTGA AACAGCCGGG GGGCTCCGAT AAGAACCAGA CCCCCACCTA CTGCCGAGCC 
ATCCCTCTGC TCCATCCTCA CCCCAGTGCT GCTCCCCCCT ACACTGTCAT TGTGCAGCCC 
CCCCAGGAAC AGACTCTCTC CAGGCAGAGC CCCCCAGCCC TGGCACATTC TGTTCCTCTA 
GCTCAGGGCT ACACGAATAG AACCCCCCCA GAGGCGCCTT TACAGGGGGG GAGACACGAG 
GGAGACGGCA CGTCGCACAG CGAGGATCAT TCGGCCCAGG AGCGGCACCC CAAACGGCTC 
CCCGAGTCCG ACCGCGGCTG CACCTCGAAA AGGATGAAAA GTTCTGCGGT AGATGATGTG 
ACAGAGACTC TCTATCCGTC TGGTTATCTA ATTCCCATAC ATTTGGCCCC AGTGGCCCCT 
GAACCCAGTA AAGAGAACAC GGGCCCCTCC TCGGAGAACA AGCTCTTTAC CTCCCCAATT 
CCAGGAGTCT TCCCTCTCAA GCTTATGTTC TCTCCCGGCC CGGTGACGGC GGTTCCGGTT 
ATGAGCCGCG GCGGGCAGCA CGTTGGAGGT GGGTCGGGTT CTGCCTCTAG GAGCCCCAGC 
CCGGGAATGT TCACTTTCGC CCTACAGAAC AGAGAGCTGA TCTCGGCAGG ACTCCCCCAA 
GGGGCTACCG TGTCACCGCG CAATGGGAGG GGCCAAGAGG AGCTGTCTGC GGCTTCAGTG 
TTAAACTGTA AACATGTGTC CCCAGTGCCT TATCACGGGC AGCCATTCAC CGTATTTGCC 
TTGCAGCAGT CGGCCGTTCC TGTGACCCCC AAAGGATATC ACTCCCTGCA GGAAACGTTC 
TTCCGTACCC CGGGGGGCAT GGGCTGCTCC CCCCCAGAAT CTGCACGGAA GCTGGATGTC 
GGCACAGACG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002942881.1
CDS	242..2476
Translation	MEEGSKENCGFNGSPMGSRSPPKQLTSAASVLGEIQIAAANLKTPTKPQERNNADPWTPTANLKMLISAASPEIRNREREILEEQFSGDELEKTLPSRKEKSLGLLCHKFLARYPSYPNPAVNNSICLDEVAGELSVERRRIYDIVNVLESLHMVSRLAKNKYIWHGRLNLSKTFDALKKVGEENRYGEQIQLLRKREQEECDSQNSPNAETQKPLAKQPEVGFVELPGLEFRAASVNSRKEKSLRVMSQRFVMLFLVSDPQIVSLEVAAKILIGEDQLEDLDKSKFKTKIRRLYDIANVLTSLNLIKKVHVTEEKGRKPAFQWTCPELCTDDQENRSSPAALTPVAIDLSSPKENCAKNLFASGGKTFTRHPSLIKLAKSIENDRRKINSAPSSPIKSGDGSSSAASKMAQLAAICKQQLQQSRDQTKVKLKVSACKAKSTVKQPGGSDKNQTPTYCRAIPLLHPHPSAAPPYTVIVQPPQEQTLSRQSPPALAHSVPLAQGYTNRTPPEAPLQGGRHEGDGTSHSEDHSAQERHPKRLPESDRGCTSKRMKSSAVDDVTETLYPSGYLIPIHLAPVAPEPSKENTGPSSENKLFTSPIPGVFPLKLMFSPGPVTAVPVMSRGGQHVGGGSGSASRSPSPGMFTFALQNRELISAGLPQGATVSPRNGRGQEELSAASVLNCKHVSPVPYHGQPFTVFALQQSAVPVTPKGYHSLQETFFRTPGGMGCSPPESARKLDVGTDD

Target ORF information:

RefSeq Version	XM_002942835.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis E2F transcription factor 8 (e2f8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002942835.5

ORF Insert Sequence:

ATGGAGGAGG GATCTAAGGA AAACTGTGGG TTCAATGGAA GTCCCATGGG CAGCAGGTCC 
CCCCCAAAGC AGCTCACATC TGCAGCCTCA GTGTTGGGGG AGATCCAGAT TGCAGCCGCC 
AATTTAAAGA CCCCTACAAA GCCTCAGGAA CGGAACAATG CCGACCCCTG GACCCCAACG 
GCCAATCTCA AAATGCTGAT CAGCGCCGCC AGCCCCGAGA TCCGGAACCG GGAGCGAGAG 
ATCTTGGAGG AGCAGTTTTC TGGAGATGAA CTGGAAAAGA CGCTGCCGAG CCGGAAGGAG 
AAGAGTTTGG GGTTACTGTG CCATAAGTTC CTGGCACGCT ACCCCAGTTA CCCCAATCCT 
GCTGTGAATA ACAGTATCTG CCTGGATGAA GTTGCTGGGG AGCTGAGCGT CGAGCGCAGG 
CGCATCTACG ACATCGTCAA CGTGCTGGAG AGTCTGCATA TGGTCAGCCG CTTGGCCAAA 
AACAAGTACA TCTGGCACGG CCGGCTCAAT CTGAGCAAAA CGTTCGACGC GCTGAAGAAG 
GTCGGCGAGG AGAACCGATA CGGGGAGCAG ATCCAGCTGC TGCGGAAGCG AGAGCAGGAA 
GAATGCGATT CCCAGAATTC CCCCAATGCG GAAACGCAAA AGCCTCTTGC TAAGCAGCCG 
GAAGTCGGGT TTGTGGAGCT GCCCGGATTG GAGTTCCGAG CTGCGTCGGT GAATAGCAGG 
AAAGAGAAGT CCCTGCGAGT AATGAGCCAG CGCTTCGTGA TGCTGTTCCT TGTGTCGGAC 
CCTCAGATCG TTAGCCTGGA AGTTGCTGCT AAAATATTAA TTGGGGAGGA CCAACTGGAG 
GATTTGGACA AAAGCAAATT TAAAACGAAG ATCCGGCGCC TGTACGATAT CGCCAATGTC 
CTGACCAGCC TCAATCTCAT AAAGAAAGTC CACGTGACGG AGGAGAAGGG GAGGAAGCCG 
GCTTTCCAGT GGACGTGCCC GGAGTTGTGT ACAGACGACC AAGAGAACAG ATCCAGCCCC 
GCTGCACTTA CCCCCGTGGC CATAGACCTG AGCTCCCCAA AGGAGAACTG TGCCAAAAAC 
CTCTTTGCCT CCGGAGGCAA AACCTTTACC CGCCACCCGT CTCTGATAAA ACTCGCCAAA 
AGCATCGAGA ACGACCGCAG GAAGATCAAC TCGGCGCCCT CCAGCCCCAT AAAGAGCGGA 
GACGGGTCCT CCAGCGCAGC CTCCAAAATG GCCCAACTTG CAGCGATTTG CAAACAGCAG 
CTCCAGCAGT CCAGGGATCA GACTAAGGTG AAGCTGAAGG TCTCCGCTTG CAAAGCCAAA 
TCCACCGTGA AACAGCCGGG GGGCTCCGAT AAGAACCAGA CCCCCACCTA CTGCCGAGCC 
ATCCCTCTGC TCCATCCTCA CCCCAGTGCT GCTCCCCCCT ACACTGTCAT TGTGCAGCCC 
CCCCAGGAAC AGACTCTCTC CAGGCAGAGC CCCCCAGCCC TGGCACATTC TGTTCCTCTA 
GCTCAGGGCT ACACGAATAG AACCCCCCCA GAGGCGCCTT TACAGGGGGG GAGACACGAG 
GGAGACGGCA CGTCGCACAG CGAGGATCAT TCGGCCCAGG AGCGGCACCC CAAACGGCTC 
CCCGAGTCCG ACCGCGGCTG CACCTCGAAA AGGATGAAAA GTTCTGCGGT AGATGATGTG 
ACAGAGACTC TCTATCCGTC TGGTTATCTA ATTCCCATAC ATTTGGCCCC AGTGGCCCCT 
GAACCCAGTA AAGAGAACAC GGGCCCCTCC TCGGAGAACA AGCTCTTTAC CTCCCCAATT 
CCAGGAGTCT TCCCTCTCAA GCTTATGTTC TCTCCCGGCC CGGTGACGGC GGTTCCGGTT 
ATGAGCCGCG GCGGGCAGCA CGTTGGAGGT GGGTCGGGTT CTGCCTCTAG GAGCCCCAGC 
CCGGGAATGT TCACTTTCGC CCTACAGAAC AGAGAGCTGA TCTCGGCAGG ACTCCCCCAA 
GGGGCTACCG TGTCACCGCG CAATGGGAGG GGCCAAGAGG AGCTGTCTGC GGCTTCAGTG 
TTAAACTGTA AACATGTGTC CCCAGTGCCT TATCACGGGC AGCCATTCAC CGTATTTGCC 
TTGCAGCAGT CGGCCGTTCC TGTGACCCCC AAAGGATATC ACTCCCTGCA GGAAACGTTC 
TTCCGTACCC CGGGGGGCAT GGGCTGCTCC CCCCCAGAAT CTGCACGGAA GCTGGATGTC 
GGCACAGACG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

e2f8 ( XM_004913337.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913394.1 Xenopus tropicalis E2F transcription factor 8 (e2f8), transcript variant X1, mRNA.

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CloneID	OXa08473
Clone ID Related Accession (Same CDS sequence)	XM_002942835.5 , XM_004913337.3 , XM_018090118.1 , XM_002942835.4 , XM_004913337.4
Accession Version	XM_004913337.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transcription factor E2F8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004913337.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG GATCTAAGGA AAACTGTGGG TTCAATGGAA GTCCCATGGG CAGCAGGTCC 
CCCCCAAAGC AGCTCACATC TGCAGCCTCA GTGTTGGGGG AGATCCAGAT TGCAGCCGCC 
AATTTAAAGA CCCCTACAAA GCCTCAGGAA CGGAACAATG CCGACCCCTG GACCCCAACG 
GCCAATCTCA AAATGCTGAT CAGCGCCGCC AGCCCCGAGA TCCGGAACCG GGAGCGAGAG 
ATCTTGGAGG AGCAGTTTTC TGGAGATGAA CTGGAAAAGA CGCTGCCGAG CCGGAAGGAG 
AAGAGTTTGG GGTTACTGTG CCATAAGTTC CTGGCACGCT ACCCCAGTTA CCCCAATCCT 
GCTGTGAATA ACAGTATCTG CCTGGATGAA GTTGCTGGGG AGCTGAGCGT CGAGCGCAGG 
CGCATCTACG ACATCGTCAA CGTGCTGGAG AGTCTGCATA TGGTCAGCCG CTTGGCCAAA 
AACAAGTACA TCTGGCACGG CCGGCTCAAT CTGAGCAAAA CGTTCGACGC GCTGAAGAAG 
GTCGGCGAGG AGAACCGATA CGGGGAGCAG ATCCAGCTGC TGCGGAAGCG AGAGCAGGAA 
GAATGCGATT CCCAGAATTC CCCCAATGCG GAAACGCAAA AGCCTCTTGC TAAGCAGCCG 
GAAGTCGGGT TTGTGGAGCT GCCCGGATTG GAGTTCCGAG CTGCGTCGGT GAATAGCAGG 
AAAGAGAAGT CCCTGCGAGT AATGAGCCAG CGCTTCGTGA TGCTGTTCCT TGTGTCGGAC 
CCTCAGATCG TTAGCCTGGA AGTTGCTGCT AAAATATTAA TTGGGGAGGA CCAACTGGAG 
GATTTGGACA AAAGCAAATT TAAAACGAAG ATCCGGCGCC TGTACGATAT CGCCAATGTC 
CTGACCAGCC TCAATCTCAT AAAGAAAGTC CACGTGACGG AGGAGAAGGG GAGGAAGCCG 
GCTTTCCAGT GGACGTGCCC GGAGTTGTGT ACAGACGACC AAGAGAACAG ATCCAGCCCC 
GCTGCACTTA CCCCCGTGGC CATAGACCTG AGCTCCCCAA AGGAGAACTG TGCCAAAAAC 
CTCTTTGCCT CCGGAGGCAA AACCTTTACC CGCCACCCGT CTCTGATAAA ACTCGCCAAA 
AGCATCGAGA ACGACCGCAG GAAGATCAAC TCGGCGCCCT CCAGCCCCAT AAAGAGCGGA 
GACGGGTCCT CCAGCGCAGC CTCCAAAATG GCCCAACTTG CAGCGATTTG CAAACAGCAG 
CTCCAGCAGT CCAGGGATCA GACTAAGGTG AAGCTGAAGG TCTCCGCTTG CAAAGCCAAA 
TCCACCGTGA AACAGCCGGG GGGCTCCGAT AAGAACCAGA CCCCCACCTA CTGCCGAGCC 
ATCCCTCTGC TCCATCCTCA CCCCAGTGCT GCTCCCCCCT ACACTGTCAT TGTGCAGCCC 
CCCCAGGAAC AGACTCTCTC CAGGCAGAGC CCCCCAGCCC TGGCACATTC TGTTCCTCTA 
GCTCAGGGCT ACACGAATAG AACCCCCCCA GAGGCGCCTT TACAGGGGGG GAGACACGAG 
GGAGACGGCA CGTCGCACAG CGAGGATCAT TCGGCCCAGG AGCGGCACCC CAAACGGCTC 
CCCGAGTCCG ACCGCGGCTG CACCTCGAAA AGGATGAAAA GTTCTGCGGT AGATGATGTG 
ACAGAGACTC TCTATCCGTC TGGTTATCTA ATTCCCATAC ATTTGGCCCC AGTGGCCCCT 
GAACCCAGTA AAGAGAACAC GGGCCCCTCC TCGGAGAACA AGCTCTTTAC CTCCCCAATT 
CCAGGAGTCT TCCCTCTCAA GCTTATGTTC TCTCCCGGCC CGGTGACGGC GGTTCCGGTT 
ATGAGCCGCG GCGGGCAGCA CGTTGGAGGT GGGTCGGGTT CTGCCTCTAG GAGCCCCAGC 
CCGGGAATGT TCACTTTCGC CCTACAGAAC AGAGAGCTGA TCTCGGCAGG ACTCCCCCAA 
GGGGCTACCG TGTCACCGCG CAATGGGAGG GGCCAAGAGG AGCTGTCTGC GGCTTCAGTG 
TTAAACTGTA AACATGTGTC CCCAGTGCCT TATCACGGGC AGCCATTCAC CGTATTTGCC 
TTGCAGCAGT CGGCCGTTCC TGTGACCCCC AAAGGATATC ACTCCCTGCA GGAAACGTTC 
TTCCGTACCC CGGGGGGCAT GGGCTGCTCC CCCCCAGAAT CTGCACGGAA GCTGGATGTC 
GGCACAGACG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913394.1
CDS	178..2412
Translation	MEEGSKENCGFNGSPMGSRSPPKQLTSAASVLGEIQIAAANLKTPTKPQERNNADPWTPTANLKMLISAASPEIRNREREILEEQFSGDELEKTLPSRKEKSLGLLCHKFLARYPSYPNPAVNNSICLDEVAGELSVERRRIYDIVNVLESLHMVSRLAKNKYIWHGRLNLSKTFDALKKVGEENRYGEQIQLLRKREQEECDSQNSPNAETQKPLAKQPEVGFVELPGLEFRAASVNSRKEKSLRVMSQRFVMLFLVSDPQIVSLEVAAKILIGEDQLEDLDKSKFKTKIRRLYDIANVLTSLNLIKKVHVTEEKGRKPAFQWTCPELCTDDQENRSSPAALTPVAIDLSSPKENCAKNLFASGGKTFTRHPSLIKLAKSIENDRRKINSAPSSPIKSGDGSSSAASKMAQLAAICKQQLQQSRDQTKVKLKVSACKAKSTVKQPGGSDKNQTPTYCRAIPLLHPHPSAAPPYTVIVQPPQEQTLSRQSPPALAHSVPLAQGYTNRTPPEAPLQGGRHEGDGTSHSEDHSAQERHPKRLPESDRGCTSKRMKSSAVDDVTETLYPSGYLIPIHLAPVAPEPSKENTGPSSENKLFTSPIPGVFPLKLMFSPGPVTAVPVMSRGGQHVGGGSGSASRSPSPGMFTFALQNRELISAGLPQGATVSPRNGRGQEELSAASVLNCKHVSPVPYHGQPFTVFALQQSAVPVTPKGYHSLQETFFRTPGGMGCSPPESARKLDVGTDD

Target ORF information:

RefSeq Version	XM_004913337.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis E2F transcription factor 8 (e2f8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913337.4

ORF Insert Sequence:

ATGGAGGAGG GATCTAAGGA AAACTGTGGG TTCAATGGAA GTCCCATGGG CAGCAGGTCC 
CCCCCAAAGC AGCTCACATC TGCAGCCTCA GTGTTGGGGG AGATCCAGAT TGCAGCCGCC 
AATTTAAAGA CCCCTACAAA GCCTCAGGAA CGGAACAATG CCGACCCCTG GACCCCAACG 
GCCAATCTCA AAATGCTGAT CAGCGCCGCC AGCCCCGAGA TCCGGAACCG GGAGCGAGAG 
ATCTTGGAGG AGCAGTTTTC TGGAGATGAA CTGGAAAAGA CGCTGCCGAG CCGGAAGGAG 
AAGAGTTTGG GGTTACTGTG CCATAAGTTC CTGGCACGCT ACCCCAGTTA CCCCAATCCT 
GCTGTGAATA ACAGTATCTG CCTGGATGAA GTTGCTGGGG AGCTGAGCGT CGAGCGCAGG 
CGCATCTACG ACATCGTCAA CGTGCTGGAG AGTCTGCATA TGGTCAGCCG CTTGGCCAAA 
AACAAGTACA TCTGGCACGG CCGGCTCAAT CTGAGCAAAA CGTTCGACGC GCTGAAGAAG 
GTCGGCGAGG AGAACCGATA CGGGGAGCAG ATCCAGCTGC TGCGGAAGCG AGAGCAGGAA 
GAATGCGATT CCCAGAATTC CCCCAATGCG GAAACGCAAA AGCCTCTTGC TAAGCAGCCG 
GAAGTCGGGT TTGTGGAGCT GCCCGGATTG GAGTTCCGAG CTGCGTCGGT GAATAGCAGG 
AAAGAGAAGT CCCTGCGAGT AATGAGCCAG CGCTTCGTGA TGCTGTTCCT TGTGTCGGAC 
CCTCAGATCG TTAGCCTGGA AGTTGCTGCT AAAATATTAA TTGGGGAGGA CCAACTGGAG 
GATTTGGACA AAAGCAAATT TAAAACGAAG ATCCGGCGCC TGTACGATAT CGCCAATGTC 
CTGACCAGCC TCAATCTCAT AAAGAAAGTC CACGTGACGG AGGAGAAGGG GAGGAAGCCG 
GCTTTCCAGT GGACGTGCCC GGAGTTGTGT ACAGACGACC AAGAGAACAG ATCCAGCCCC 
GCTGCACTTA CCCCCGTGGC CATAGACCTG AGCTCCCCAA AGGAGAACTG TGCCAAAAAC 
CTCTTTGCCT CCGGAGGCAA AACCTTTACC CGCCACCCGT CTCTGATAAA ACTCGCCAAA 
AGCATCGAGA ACGACCGCAG GAAGATCAAC TCGGCGCCCT CCAGCCCCAT AAAGAGCGGA 
GACGGGTCCT CCAGCGCAGC CTCCAAAATG GCCCAACTTG CAGCGATTTG CAAACAGCAG 
CTCCAGCAGT CCAGGGATCA GACTAAGGTG AAGCTGAAGG TCTCCGCTTG CAAAGCCAAA 
TCCACCGTGA AACAGCCGGG GGGCTCCGAT AAGAACCAGA CCCCCACCTA CTGCCGAGCC 
ATCCCTCTGC TCCATCCTCA CCCCAGTGCT GCTCCCCCCT ACACTGTCAT TGTGCAGCCC 
CCCCAGGAAC AGACTCTCTC CAGGCAGAGC CCCCCAGCCC TGGCACATTC TGTTCCTCTA 
GCTCAGGGCT ACACGAATAG AACCCCCCCA GAGGCGCCTT TACAGGGGGG GAGACACGAG 
GGAGACGGCA CGTCGCACAG CGAGGATCAT TCGGCCCAGG AGCGGCACCC CAAACGGCTC 
CCCGAGTCCG ACCGCGGCTG CACCTCGAAA AGGATGAAAA GTTCTGCGGT AGATGATGTG 
ACAGAGACTC TCTATCCGTC TGGTTATCTA ATTCCCATAC ATTTGGCCCC AGTGGCCCCT 
GAACCCAGTA AAGAGAACAC GGGCCCCTCC TCGGAGAACA AGCTCTTTAC CTCCCCAATT 
CCAGGAGTCT TCCCTCTCAA GCTTATGTTC TCTCCCGGCC CGGTGACGGC GGTTCCGGTT 
ATGAGCCGCG GCGGGCAGCA CGTTGGAGGT GGGTCGGGTT CTGCCTCTAG GAGCCCCAGC 
CCGGGAATGT TCACTTTCGC CCTACAGAAC AGAGAGCTGA TCTCGGCAGG ACTCCCCCAA 
GGGGCTACCG TGTCACCGCG CAATGGGAGG GGCCAAGAGG AGCTGTCTGC GGCTTCAGTG 
TTAAACTGTA AACATGTGTC CCCAGTGCCT TATCACGGGC AGCCATTCAC CGTATTTGCC 
TTGCAGCAGT CGGCCGTTCC TGTGACCCCC AAAGGATATC ACTCCCTGCA GGAAACGTTC 
TTCCGTACCC CGGGGGGCAT GGGCTGCTCC CCCCCAGAAT CTGCACGGAA GCTGGATGTC 
GGCACAGACG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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e2f8 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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