apaf1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following apaf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the apaf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa54587 XM_031898854.1
Latest version!
Xenopus tropicalis apoptotic peptidase activating factor 1 (apaf1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa54587 XM_012959843.3
Latest version!
Xenopus tropicalis apoptotic peptidase activating factor 1 (apaf1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa31035 XM_012959843.2
Latest version!
Xenopus tropicalis apoptotic peptidase activating factor 1 (apaf1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa54587
    Clone ID Related Accession (Same CDS sequence) XM_031898854.1 , XM_012959843.3
    Accession Version XM_031898854.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3747bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product apoptotic protease-activating factor 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATGAGA AGGCTCGGAG TCTACTGCTT CAGAATCGAA CAGCACTTGT GAGGGACATT 
    CGCACAGCTT ACATTATGGA TCATATGATA AGTGATCAGG TTATTACCCC AGAGGAGGAG
    GCCCAAGTAA AATCTCAGCG TACTCAGATG GACAAAGCAA ATTTCCTTAT CAATTTAATT
    CTTGGCAAAA GCAATCAGGC ATATGTTTCT TTCTATAATG CTTTGCTTCA TGAGGGGTAC
    AAAGACCTTG CTGTTCTTCT TAAAGAGGCC GCTAATGCTG AGCTTTCTGA CAGTACCAAG
    AGTTCAAGCA ATGGAATTAC TTCATATGTT AAAACAGTAC TATGTGAGGG TGCTGTACCT
    CAGAGACCAG TTGTTTTTGT CACAAGGCTG AAGCTTGTCA GTAAGATACA ACAGAGCCTG
    TATAAGTTGA GTGTGGAATC CGGATGGGTA ACCGTGTATG GAATGGCAGG CTGCGGAAAA
    ACTGTCCTGG CTGCAGAAGC ACTTCGAGAC CACAAAATAC TGAATGAATG TTTTCCTGGA
    GGAGTCCACT GGGTCTCTGT TGGAAGGCAA GACAAAGCCG GGCTCCTGAT GAAACTACAA
    AATTTGTGCA CCCGCCTAGA TCAAGAGGCC AAGTATTCAC AGAGACCTCC ATTAAATGCG
    GAAGAAGCCA GAGAGAGACT GAGGCTGATG GTGAACCGTA TGTACCCTAG GTGCCTCATT
    GTTCTGGATG ATGTTTGGGA TTCTTGGGTG CTCAAAGCTT TTGATATTCA GTGTCGGGTG
    CTCATTACCA GCAGAGACAA GAGTGTGACT GATTCACTGC CTGGCTTTAA GGAGGCTGTT
    CATGTAGACA GTGAGCTTGA GCATTCAAAA GGCCTTGAAA TACTCTCACG CTTCCTGAAT
    ATAAAAGCAG AAGCACTGCC ATCCCAGGCC CATAACATAA TTAAAGAGTC CAAAGGTTCT
    CCTCTTGTAG TATCTCTGAT TGGAGCTCTG CTACGGGAAT TTCCCACCCG CTGGGATTTC
    TACCTCACTC AGCTAAAGAG AAAGCAATTT AAAAGAATAA GGAAGTCTTC CTCCTATGAT
    TACGAGGCTC TCGACGAAGC AATGTCAATA AGTGTGGATA ATCTAAAAGA AGACTTTAAA
    GAGTACTACA ATGATTTCTC CATTATAGAA AAGGATGTGA AAGTTCCCAC ACAGGTTCTG
    TGTATCTTAT GGGACATGGA ACGTGAAGAT GTGGAAGACA TGATGCAGGA GTTTGTCAAC
    AAGTCCTTAG TATACTGTGA TCGTAATGGA AAGTCCTATT CATACTATTT ACATGATCTC
    CAACTGGATT ATCTTACAGA GAGGAATCGG GACCGACTGA CTGTCCTTCA CTCCAAACTT
    GTTGACCAAT ATAAAAAACA CTATTCAAGT AAATCACCTG CTGTGGATCA AGAGGATTCT
    GTGTATTGGT ATCATTACCT TGCTTACCAC ATGGCCAAAG CCAATATGCA CCAAGATCTT
    TGCTCACTCT TGTTTTCCCT GAATTGGTTA AAAGCTAAAA CAGAATTATT TGGGACAGCC
    CACCTCATTC ATGAGTTTGT ACAGTATCGG AATATTCTTG ATAATCAGAA TGCAAAAGCC
    CGAGAACATT TTCAAGAATT TATTGCTGTG AATGGTCATC TTCTTGGGTC GAATCCGGAC
    GTTAGTATTG TGCAGCTGGG CCTTGGTCAG GCTGAAGACT CAGAAGTCTA CAAGCAAGCT
    ATTCTGCAAG CCAAAGAAGA ACTCCAATCT GGAGCGCTCT ATGTCAACTG GATAAACAAG
    CAAAGTATGA AGAACCTGCC AACTTTAGTT GTTCGTCCAC ATAAAGATGC TGTTTTCCAT
    GCATGCTTTT CTTCTGATGG ACAAAGAATT GCTTCTTGTG GAGCTGATAA AACTTTACAG
    GTGTTCAAGA GTGAAACAGG GGAAAAACTC CTTGAAATAG AAGCACATGA TGATGAAGTG
    CTGTGTTGTG CATTTTCTGC AGATGAAAAG CTTCTTGCCA CCTGTTCGGC AGACAGGAAA
    GTAAAGATTT GGAATGCCAA GACAGGAAAA CCCTTAAGAG TGTATGAAGA GCATATAGAA
    CAAGTCAACT GCTGCCAGTT CACCAACGGC CTCTCTGCAC CACTGCTTGC CACATGCTCC
    AATGACTGCT TAATCAAGCT GTGGAATTCT GAGAAGAACT ATTGCCATAA CACTCTTATT
    GGGCACATAG AATCTGTCTA CCATTGCAGA TATTCACCAG ATGATCGGTA TTTAGCCAGT
    TCCTCTATGG ATGGCTCATT AAAGATTTGG GATGTAGAGT CTGCAAATGA GGAGAAGTCA
    ATTGAAGTTG CAAAGCTTTT TGAAAATGAA GATGAAGCGC AGCCTGAAGT ACTACTTAAA
    TGTTGCGCTT GGTCAAATGA TGGTTCAAGG ATTATGGTAA CAGCTCGCAA TTTCCTGTGT
    ATTTATGATG CTGAGAGATG CAACCTTTTG TCTCAACTCA AGGCCTGTCA TCAGATCCTT
    TACTGTGACT TCTGCACCAC TAATCAGATT GCAGCCCTTG CTTTGTCACA CTACGTAGTT
    CAGCTGTGGG ACATAGATTC CTCTACAAAG ATAGCTGACT TCAATGCACA CCTGAGCTGG
    GTTCACTGTG TGAAATTTTC TCCAAAGAGT TCTTCATTCC TGACATCTTC TGATGACCAG
    ACTGTGAAGC TTTGGGAAAC TAGTAATGTC AGCAAACCAG CAGCCACTAA CTTGAAGCGT
    GAGTTTGATG TGACCTTCAG TGGTGAGAGA ACACTGGTGC TTGCAACAAG CAAAGATGAT
    TGTATACTAC TAATTAATGG AATGACTGGA GAGACCTTAT CACAGATCAG AGCACAGGAC
    AATTGTGTTA CGTGCTGCTG TCTCAGTACT GACTATCAGC TAGCAGCTAT TGGGCATAGA
    GATGGAAAAG TAAAGATTTT AGATTTATCC GGCGGTGAAA TCCTTCATAA ACTGGATGGT
    CACAGAGACA CAGTTCAGCA TTGCCAGTTC ACTGCAGATG GAAAACTCCT TGTTTCAAGC
    TCTGATGATT GCACTGTTCG AGTCTGGAAC TTGGTATCTG GAAAGAACTT AGAATTATGT
    GGACACAAAG AACCTGTAAA ATTCTTCAAG ATTTTACAAG AGTCAAAAGT CTTTTCTTGG
    TCTTTTGATG GAACAGTCAA GGTATGGAAT CTCCTCACTG GGCAGCTCAT AAAAGAGTTT
    ATTTGCCATT CTGCAACAGT TCTGGCTTGT GATATCTCAC CTGACTCCAC CAAATTTTCA
    TCTGCCTCTA CTGATAAAAG TGCCAAGATA TGGAGCTTAG ATAACTGTGC CCTCCTCCAT
    GAATTGAATA AACACCAAAG CTGTGTACGA TGCTGTCGGT TTTCATGGGA TAACCAGTAT
    TTGGCAACCG GTGATGACAG TGGTAAAATT ATGATTTGGA GTGTGCAGAA TGGAGAGCTG
    CTAAGAGAGT GTTGTGACGT ATCAGTGAAT AATGAAAACA GTCTGCATGA TGGTTGGGTG
    ACAGATCTAC ACTTCTCTCC TGACAACAAG CTAATTGTAT CCTCTGGGGC AAATATCAAG
    TGGTGGGAAG TGGACACTGG AGCCTGCAAA CAGACATTCT ACACAATTGG CACAAATCTC
    AAATCCATCC ATGTATGTTC TATATTTAGA ACCTTTGTAA CAATTGACAA TCTGGGAATT
    CTGTATGTGC TGCAGCTAAT AGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754714.1
    CDS429..4175
    Translation

    Target ORF information:

    RefSeq Version XM_031898854.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis apoptotic peptidase activating factor 1 (apaf1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898854.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATGAGA AGGCTCGGAG TCTACTGCTT CAGAATCGAA CAGCACTTGT GAGGGACATT 
    CGCACAGCTT ACATTATGGA TCATATGATA AGTGATCAGG TTATTACCCC AGAGGAGGAG
    GCCCAAGTAA AATCTCAGCG TACTCAGATG GACAAAGCAA ATTTCCTTAT CAATTTAATT
    CTTGGCAAAA GCAATCAGGC ATATGTTTCT TTCTATAATG CTTTGCTTCA TGAGGGGTAC
    AAAGACCTTG CTGTTCTTCT TAAAGAGGCC GCTAATGCTG AGCTTTCTGA CAGTACCAAG
    AGTTCAAGCA ATGGAATTAC TTCATATGTT AAAACAGTAC TATGTGAGGG TGCTGTACCT
    CAGAGACCAG TTGTTTTTGT CACAAGGCTG AAGCTTGTCA GTAAGATACA ACAGAGCCTG
    TATAAGTTGA GTGTGGAATC CGGATGGGTA ACCGTGTATG GAATGGCAGG CTGCGGAAAA
    ACTGTCCTGG CTGCAGAAGC ACTTCGAGAC CACAAAATAC TGAATGAATG TTTTCCTGGA
    GGAGTCCACT GGGTCTCTGT TGGAAGGCAA GACAAAGCCG GGCTCCTGAT GAAACTACAA
    AATTTGTGCA CCCGCCTAGA TCAAGAGGCC AAGTATTCAC AGAGACCTCC ATTAAATGCG
    GAAGAAGCCA GAGAGAGACT GAGGCTGATG GTGAACCGTA TGTACCCTAG GTGCCTCATT
    GTTCTGGATG ATGTTTGGGA TTCTTGGGTG CTCAAAGCTT TTGATATTCA GTGTCGGGTG
    CTCATTACCA GCAGAGACAA GAGTGTGACT GATTCACTGC CTGGCTTTAA GGAGGCTGTT
    CATGTAGACA GTGAGCTTGA GCATTCAAAA GGCCTTGAAA TACTCTCACG CTTCCTGAAT
    ATAAAAGCAG AAGCACTGCC ATCCCAGGCC CATAACATAA TTAAAGAGTC CAAAGGTTCT
    CCTCTTGTAG TATCTCTGAT TGGAGCTCTG CTACGGGAAT TTCCCACCCG CTGGGATTTC
    TACCTCACTC AGCTAAAGAG AAAGCAATTT AAAAGAATAA GGAAGTCTTC CTCCTATGAT
    TACGAGGCTC TCGACGAAGC AATGTCAATA AGTGTGGATA ATCTAAAAGA AGACTTTAAA
    GAGTACTACA ATGATTTCTC CATTATAGAA AAGGATGTGA AAGTTCCCAC ACAGGTTCTG
    TGTATCTTAT GGGACATGGA ACGTGAAGAT GTGGAAGACA TGATGCAGGA GTTTGTCAAC
    AAGTCCTTAG TATACTGTGA TCGTAATGGA AAGTCCTATT CATACTATTT ACATGATCTC
    CAACTGGATT ATCTTACAGA GAGGAATCGG GACCGACTGA CTGTCCTTCA CTCCAAACTT
    GTTGACCAAT ATAAAAAACA CTATTCAAGT AAATCACCTG CTGTGGATCA AGAGGATTCT
    GTGTATTGGT ATCATTACCT TGCTTACCAC ATGGCCAAAG CCAATATGCA CCAAGATCTT
    TGCTCACTCT TGTTTTCCCT GAATTGGTTA AAAGCTAAAA CAGAATTATT TGGGACAGCC
    CACCTCATTC ATGAGTTTGT ACAGTATCGG AATATTCTTG ATAATCAGAA TGCAAAAGCC
    CGAGAACATT TTCAAGAATT TATTGCTGTG AATGGTCATC TTCTTGGGTC GAATCCGGAC
    GTTAGTATTG TGCAGCTGGG CCTTGGTCAG GCTGAAGACT CAGAAGTCTA CAAGCAAGCT
    ATTCTGCAAG CCAAAGAAGA ACTCCAATCT GGAGCGCTCT ATGTCAACTG GATAAACAAG
    CAAAGTATGA AGAACCTGCC AACTTTAGTT GTTCGTCCAC ATAAAGATGC TGTTTTCCAT
    GCATGCTTTT CTTCTGATGG ACAAAGAATT GCTTCTTGTG GAGCTGATAA AACTTTACAG
    GTGTTCAAGA GTGAAACAGG GGAAAAACTC CTTGAAATAG AAGCACATGA TGATGAAGTG
    CTGTGTTGTG CATTTTCTGC AGATGAAAAG CTTCTTGCCA CCTGTTCGGC AGACAGGAAA
    GTAAAGATTT GGAATGCCAA GACAGGAAAA CCCTTAAGAG TGTATGAAGA GCATATAGAA
    CAAGTCAACT GCTGCCAGTT CACCAACGGC CTCTCTGCAC CACTGCTTGC CACATGCTCC
    AATGACTGCT TAATCAAGCT GTGGAATTCT GAGAAGAACT ATTGCCATAA CACTCTTATT
    GGGCACATAG AATCTGTCTA CCATTGCAGA TATTCACCAG ATGATCGGTA TTTAGCCAGT
    TCCTCTATGG ATGGCTCATT AAAGATTTGG GATGTAGAGT CTGCAAATGA GGAGAAGTCA
    ATTGAAGTTG CAAAGCTTTT TGAAAATGAA GATGAAGCGC AGCCTGAAGT ACTACTTAAA
    TGTTGCGCTT GGTCAAATGA TGGTTCAAGG ATTATGGTAA CAGCTCGCAA TTTCCTGTGT
    ATTTATGATG CTGAGAGATG CAACCTTTTG TCTCAACTCA AGGCCTGTCA TCAGATCCTT
    TACTGTGACT TCTGCACCAC TAATCAGATT GCAGCCCTTG CTTTGTCACA CTACGTAGTT
    CAGCTGTGGG ACATAGATTC CTCTACAAAG ATAGCTGACT TCAATGCACA CCTGAGCTGG
    GTTCACTGTG TGAAATTTTC TCCAAAGAGT TCTTCATTCC TGACATCTTC TGATGACCAG
    ACTGTGAAGC TTTGGGAAAC TAGTAATGTC AGCAAACCAG CAGCCACTAA CTTGAAGCGT
    GAGTTTGATG TGACCTTCAG TGGTGAGAGA ACACTGGTGC TTGCAACAAG CAAAGATGAT
    TGTATACTAC TAATTAATGG AATGACTGGA GAGACCTTAT CACAGATCAG AGCACAGGAC
    AATTGTGTTA CGTGCTGCTG TCTCAGTACT GACTATCAGC TAGCAGCTAT TGGGCATAGA
    GATGGAAAAG TAAAGATTTT AGATTTATCC GGCGGTGAAA TCCTTCATAA ACTGGATGGT
    CACAGAGACA CAGTTCAGCA TTGCCAGTTC ACTGCAGATG GAAAACTCCT TGTTTCAAGC
    TCTGATGATT GCACTGTTCG AGTCTGGAAC TTGGTATCTG GAAAGAACTT AGAATTATGT
    GGACACAAAG AACCTGTAAA ATTCTTCAAG ATTTTACAAG AGTCAAAAGT CTTTTCTTGG
    TCTTTTGATG GAACAGTCAA GGTATGGAAT CTCCTCACTG GGCAGCTCAT AAAAGAGTTT
    ATTTGCCATT CTGCAACAGT TCTGGCTTGT GATATCTCAC CTGACTCCAC CAAATTTTCA
    TCTGCCTCTA CTGATAAAAG TGCCAAGATA TGGAGCTTAG ATAACTGTGC CCTCCTCCAT
    GAATTGAATA AACACCAAAG CTGTGTACGA TGCTGTCGGT TTTCATGGGA TAACCAGTAT
    TTGGCAACCG GTGATGACAG TGGTAAAATT ATGATTTGGA GTGTGCAGAA TGGAGAGCTG
    CTAAGAGAGT GTTGTGACGT ATCAGTGAAT AATGAAAACA GTCTGCATGA TGGTTGGGTG
    ACAGATCTAC ACTTCTCTCC TGACAACAAG CTAATTGTAT CCTCTGGGGC AAATATCAAG
    TGGTGGGAAG TGGACACTGG AGCCTGCAAA CAGACATTCT ACACAATTGG CACAAATCTC
    AAATCCATCC ATGTATGTTC TATATTTAGA ACCTTTGTAA CAATTGACAA TCTGGGAATT
    CTGTATGTGC TGCAGCTAAT AGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54587
    Clone ID Related Accession (Same CDS sequence) XM_031898854.1 , XM_012959843.3
    Accession Version XM_012959843.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3747bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product apoptotic protease-activating factor 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959843.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATGAGA AGGCTCGGAG TCTACTGCTT CAGAATCGAA CAGCACTTGT GAGGGACATT 
    CGCACAGCTT ACATTATGGA TCATATGATA AGTGATCAGG TTATTACCCC AGAGGAGGAG
    GCCCAAGTAA AATCTCAGCG TACTCAGATG GACAAAGCAA ATTTCCTTAT CAATTTAATT
    CTTGGCAAAA GCAATCAGGC ATATGTTTCT TTCTATAATG CTTTGCTTCA TGAGGGGTAC
    AAAGACCTTG CTGTTCTTCT TAAAGAGGCC GCTAATGCTG AGCTTTCTGA CAGTACCAAG
    AGTTCAAGCA ATGGAATTAC TTCATATGTT AAAACAGTAC TATGTGAGGG TGCTGTACCT
    CAGAGACCAG TTGTTTTTGT CACAAGGCTG AAGCTTGTCA GTAAGATACA ACAGAGCCTG
    TATAAGTTGA GTGTGGAATC CGGATGGGTA ACCGTGTATG GAATGGCAGG CTGCGGAAAA
    ACTGTCCTGG CTGCAGAAGC ACTTCGAGAC CACAAAATAC TGAATGAATG TTTTCCTGGA
    GGAGTCCACT GGGTCTCTGT TGGAAGGCAA GACAAAGCCG GGCTCCTGAT GAAACTACAA
    AATTTGTGCA CCCGCCTAGA TCAAGAGGCC AAGTATTCAC AGAGACCTCC ATTAAATGCG
    GAAGAAGCCA GAGAGAGACT GAGGCTGATG GTGAACCGTA TGTACCCTAG GTGCCTCATT
    GTTCTGGATG ATGTTTGGGA TTCTTGGGTG CTCAAAGCTT TTGATATTCA GTGTCGGGTG
    CTCATTACCA GCAGAGACAA GAGTGTGACT GATTCACTGC CTGGCTTTAA GGAGGCTGTT
    CATGTAGACA GTGAGCTTGA GCATTCAAAA GGCCTTGAAA TACTCTCACG CTTCCTGAAT
    ATAAAAGCAG AAGCACTGCC ATCCCAGGCC CATAACATAA TTAAAGAGTC CAAAGGTTCT
    CCTCTTGTAG TATCTCTGAT TGGAGCTCTG CTACGGGAAT TTCCCACCCG CTGGGATTTC
    TACCTCACTC AGCTAAAGAG AAAGCAATTT AAAAGAATAA GGAAGTCTTC CTCCTATGAT
    TACGAGGCTC TCGACGAAGC AATGTCAATA AGTGTGGATA ATCTAAAAGA AGACTTTAAA
    GAGTACTACA ATGATTTCTC CATTATAGAA AAGGATGTGA AAGTTCCCAC ACAGGTTCTG
    TGTATCTTAT GGGACATGGA ACGTGAAGAT GTGGAAGACA TGATGCAGGA GTTTGTCAAC
    AAGTCCTTAG TATACTGTGA TCGTAATGGA AAGTCCTATT CATACTATTT ACATGATCTC
    CAACTGGATT ATCTTACAGA GAGGAATCGG GACCGACTGA CTGTCCTTCA CTCCAAACTT
    GTTGACCAAT ATAAAAAACA CTATTCAAGT AAATCACCTG CTGTGGATCA AGAGGATTCT
    GTGTATTGGT ATCATTACCT TGCTTACCAC ATGGCCAAAG CCAATATGCA CCAAGATCTT
    TGCTCACTCT TGTTTTCCCT GAATTGGTTA AAAGCTAAAA CAGAATTATT TGGGACAGCC
    CACCTCATTC ATGAGTTTGT ACAGTATCGG AATATTCTTG ATAATCAGAA TGCAAAAGCC
    CGAGAACATT TTCAAGAATT TATTGCTGTG AATGGTCATC TTCTTGGGTC GAATCCGGAC
    GTTAGTATTG TGCAGCTGGG CCTTGGTCAG GCTGAAGACT CAGAAGTCTA CAAGCAAGCT
    ATTCTGCAAG CCAAAGAAGA ACTCCAATCT GGAGCGCTCT ATGTCAACTG GATAAACAAG
    CAAAGTATGA AGAACCTGCC AACTTTAGTT GTTCGTCCAC ATAAAGATGC TGTTTTCCAT
    GCATGCTTTT CTTCTGATGG ACAAAGAATT GCTTCTTGTG GAGCTGATAA AACTTTACAG
    GTGTTCAAGA GTGAAACAGG GGAAAAACTC CTTGAAATAG AAGCACATGA TGATGAAGTG
    CTGTGTTGTG CATTTTCTGC AGATGAAAAG CTTCTTGCCA CCTGTTCGGC AGACAGGAAA
    GTAAAGATTT GGAATGCCAA GACAGGAAAA CCCTTAAGAG TGTATGAAGA GCATATAGAA
    CAAGTCAACT GCTGCCAGTT CACCAACGGC CTCTCTGCAC CACTGCTTGC CACATGCTCC
    AATGACTGCT TAATCAAGCT GTGGAATTCT GAGAAGAACT ATTGCCATAA CACTCTTATT
    GGGCACATAG AATCTGTCTA CCATTGCAGA TATTCACCAG ATGATCGGTA TTTAGCCAGT
    TCCTCTATGG ATGGCTCATT AAAGATTTGG GATGTAGAGT CTGCAAATGA GGAGAAGTCA
    ATTGAAGTTG CAAAGCTTTT TGAAAATGAA GATGAAGCGC AGCCTGAAGT ACTACTTAAA
    TGTTGCGCTT GGTCAAATGA TGGTTCAAGG ATTATGGTAA CAGCTCGCAA TTTCCTGTGT
    ATTTATGATG CTGAGAGATG CAACCTTTTG TCTCAACTCA AGGCCTGTCA TCAGATCCTT
    TACTGTGACT TCTGCACCAC TAATCAGATT GCAGCCCTTG CTTTGTCACA CTACGTAGTT
    CAGCTGTGGG ACATAGATTC CTCTACAAAG ATAGCTGACT TCAATGCACA CCTGAGCTGG
    GTTCACTGTG TGAAATTTTC TCCAAAGAGT TCTTCATTCC TGACATCTTC TGATGACCAG
    ACTGTGAAGC TTTGGGAAAC TAGTAATGTC AGCAAACCAG CAGCCACTAA CTTGAAGCGT
    GAGTTTGATG TGACCTTCAG TGGTGAGAGA ACACTGGTGC TTGCAACAAG CAAAGATGAT
    TGTATACTAC TAATTAATGG AATGACTGGA GAGACCTTAT CACAGATCAG AGCACAGGAC
    AATTGTGTTA CGTGCTGCTG TCTCAGTACT GACTATCAGC TAGCAGCTAT TGGGCATAGA
    GATGGAAAAG TAAAGATTTT AGATTTATCC GGCGGTGAAA TCCTTCATAA ACTGGATGGT
    CACAGAGACA CAGTTCAGCA TTGCCAGTTC ACTGCAGATG GAAAACTCCT TGTTTCAAGC
    TCTGATGATT GCACTGTTCG AGTCTGGAAC TTGGTATCTG GAAAGAACTT AGAATTATGT
    GGACACAAAG AACCTGTAAA ATTCTTCAAG ATTTTACAAG AGTCAAAAGT CTTTTCTTGG
    TCTTTTGATG GAACAGTCAA GGTATGGAAT CTCCTCACTG GGCAGCTCAT AAAAGAGTTT
    ATTTGCCATT CTGCAACAGT TCTGGCTTGT GATATCTCAC CTGACTCCAC CAAATTTTCA
    TCTGCCTCTA CTGATAAAAG TGCCAAGATA TGGAGCTTAG ATAACTGTGC CCTCCTCCAT
    GAATTGAATA AACACCAAAG CTGTGTACGA TGCTGTCGGT TTTCATGGGA TAACCAGTAT
    TTGGCAACCG GTGATGACAG TGGTAAAATT ATGATTTGGA GTGTGCAGAA TGGAGAGCTG
    CTAAGAGAGT GTTGTGACGT ATCAGTGAAT AATGAAAACA GTCTGCATGA TGGTTGGGTG
    ACAGATCTAC ACTTCTCTCC TGACAACAAG CTAATTGTAT CCTCTGGGGC AAATATCAAG
    TGGTGGGAAG TGGACACTGG AGCCTGCAAA CAGACATTCT ACACAATTGG CACAAATCTC
    AAATCCATCC ATGTATGTTC TATATTTAGA ACCTTTGTAA CAATTGACAA TCTGGGAATT
    CTGTATGTGC TGCAGCTAAT AGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815297.2
    CDS432..4178
    Translation

    Target ORF information:

    RefSeq Version XM_012959843.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis apoptotic peptidase activating factor 1 (apaf1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959843.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATGAGA AGGCTCGGAG TCTACTGCTT CAGAATCGAA CAGCACTTGT GAGGGACATT 
    CGCACAGCTT ACATTATGGA TCATATGATA AGTGATCAGG TTATTACCCC AGAGGAGGAG
    GCCCAAGTAA AATCTCAGCG TACTCAGATG GACAAAGCAA ATTTCCTTAT CAATTTAATT
    CTTGGCAAAA GCAATCAGGC ATATGTTTCT TTCTATAATG CTTTGCTTCA TGAGGGGTAC
    AAAGACCTTG CTGTTCTTCT TAAAGAGGCC GCTAATGCTG AGCTTTCTGA CAGTACCAAG
    AGTTCAAGCA ATGGAATTAC TTCATATGTT AAAACAGTAC TATGTGAGGG TGCTGTACCT
    CAGAGACCAG TTGTTTTTGT CACAAGGCTG AAGCTTGTCA GTAAGATACA ACAGAGCCTG
    TATAAGTTGA GTGTGGAATC CGGATGGGTA ACCGTGTATG GAATGGCAGG CTGCGGAAAA
    ACTGTCCTGG CTGCAGAAGC ACTTCGAGAC CACAAAATAC TGAATGAATG TTTTCCTGGA
    GGAGTCCACT GGGTCTCTGT TGGAAGGCAA GACAAAGCCG GGCTCCTGAT GAAACTACAA
    AATTTGTGCA CCCGCCTAGA TCAAGAGGCC AAGTATTCAC AGAGACCTCC ATTAAATGCG
    GAAGAAGCCA GAGAGAGACT GAGGCTGATG GTGAACCGTA TGTACCCTAG GTGCCTCATT
    GTTCTGGATG ATGTTTGGGA TTCTTGGGTG CTCAAAGCTT TTGATATTCA GTGTCGGGTG
    CTCATTACCA GCAGAGACAA GAGTGTGACT GATTCACTGC CTGGCTTTAA GGAGGCTGTT
    CATGTAGACA GTGAGCTTGA GCATTCAAAA GGCCTTGAAA TACTCTCACG CTTCCTGAAT
    ATAAAAGCAG AAGCACTGCC ATCCCAGGCC CATAACATAA TTAAAGAGTC CAAAGGTTCT
    CCTCTTGTAG TATCTCTGAT TGGAGCTCTG CTACGGGAAT TTCCCACCCG CTGGGATTTC
    TACCTCACTC AGCTAAAGAG AAAGCAATTT AAAAGAATAA GGAAGTCTTC CTCCTATGAT
    TACGAGGCTC TCGACGAAGC AATGTCAATA AGTGTGGATA ATCTAAAAGA AGACTTTAAA
    GAGTACTACA ATGATTTCTC CATTATAGAA AAGGATGTGA AAGTTCCCAC ACAGGTTCTG
    TGTATCTTAT GGGACATGGA ACGTGAAGAT GTGGAAGACA TGATGCAGGA GTTTGTCAAC
    AAGTCCTTAG TATACTGTGA TCGTAATGGA AAGTCCTATT CATACTATTT ACATGATCTC
    CAACTGGATT ATCTTACAGA GAGGAATCGG GACCGACTGA CTGTCCTTCA CTCCAAACTT
    GTTGACCAAT ATAAAAAACA CTATTCAAGT AAATCACCTG CTGTGGATCA AGAGGATTCT
    GTGTATTGGT ATCATTACCT TGCTTACCAC ATGGCCAAAG CCAATATGCA CCAAGATCTT
    TGCTCACTCT TGTTTTCCCT GAATTGGTTA AAAGCTAAAA CAGAATTATT TGGGACAGCC
    CACCTCATTC ATGAGTTTGT ACAGTATCGG AATATTCTTG ATAATCAGAA TGCAAAAGCC
    CGAGAACATT TTCAAGAATT TATTGCTGTG AATGGTCATC TTCTTGGGTC GAATCCGGAC
    GTTAGTATTG TGCAGCTGGG CCTTGGTCAG GCTGAAGACT CAGAAGTCTA CAAGCAAGCT
    ATTCTGCAAG CCAAAGAAGA ACTCCAATCT GGAGCGCTCT ATGTCAACTG GATAAACAAG
    CAAAGTATGA AGAACCTGCC AACTTTAGTT GTTCGTCCAC ATAAAGATGC TGTTTTCCAT
    GCATGCTTTT CTTCTGATGG ACAAAGAATT GCTTCTTGTG GAGCTGATAA AACTTTACAG
    GTGTTCAAGA GTGAAACAGG GGAAAAACTC CTTGAAATAG AAGCACATGA TGATGAAGTG
    CTGTGTTGTG CATTTTCTGC AGATGAAAAG CTTCTTGCCA CCTGTTCGGC AGACAGGAAA
    GTAAAGATTT GGAATGCCAA GACAGGAAAA CCCTTAAGAG TGTATGAAGA GCATATAGAA
    CAAGTCAACT GCTGCCAGTT CACCAACGGC CTCTCTGCAC CACTGCTTGC CACATGCTCC
    AATGACTGCT TAATCAAGCT GTGGAATTCT GAGAAGAACT ATTGCCATAA CACTCTTATT
    GGGCACATAG AATCTGTCTA CCATTGCAGA TATTCACCAG ATGATCGGTA TTTAGCCAGT
    TCCTCTATGG ATGGCTCATT AAAGATTTGG GATGTAGAGT CTGCAAATGA GGAGAAGTCA
    ATTGAAGTTG CAAAGCTTTT TGAAAATGAA GATGAAGCGC AGCCTGAAGT ACTACTTAAA
    TGTTGCGCTT GGTCAAATGA TGGTTCAAGG ATTATGGTAA CAGCTCGCAA TTTCCTGTGT
    ATTTATGATG CTGAGAGATG CAACCTTTTG TCTCAACTCA AGGCCTGTCA TCAGATCCTT
    TACTGTGACT TCTGCACCAC TAATCAGATT GCAGCCCTTG CTTTGTCACA CTACGTAGTT
    CAGCTGTGGG ACATAGATTC CTCTACAAAG ATAGCTGACT TCAATGCACA CCTGAGCTGG
    GTTCACTGTG TGAAATTTTC TCCAAAGAGT TCTTCATTCC TGACATCTTC TGATGACCAG
    ACTGTGAAGC TTTGGGAAAC TAGTAATGTC AGCAAACCAG CAGCCACTAA CTTGAAGCGT
    GAGTTTGATG TGACCTTCAG TGGTGAGAGA ACACTGGTGC TTGCAACAAG CAAAGATGAT
    TGTATACTAC TAATTAATGG AATGACTGGA GAGACCTTAT CACAGATCAG AGCACAGGAC
    AATTGTGTTA CGTGCTGCTG TCTCAGTACT GACTATCAGC TAGCAGCTAT TGGGCATAGA
    GATGGAAAAG TAAAGATTTT AGATTTATCC GGCGGTGAAA TCCTTCATAA ACTGGATGGT
    CACAGAGACA CAGTTCAGCA TTGCCAGTTC ACTGCAGATG GAAAACTCCT TGTTTCAAGC
    TCTGATGATT GCACTGTTCG AGTCTGGAAC TTGGTATCTG GAAAGAACTT AGAATTATGT
    GGACACAAAG AACCTGTAAA ATTCTTCAAG ATTTTACAAG AGTCAAAAGT CTTTTCTTGG
    TCTTTTGATG GAACAGTCAA GGTATGGAAT CTCCTCACTG GGCAGCTCAT AAAAGAGTTT
    ATTTGCCATT CTGCAACAGT TCTGGCTTGT GATATCTCAC CTGACTCCAC CAAATTTTCA
    TCTGCCTCTA CTGATAAAAG TGCCAAGATA TGGAGCTTAG ATAACTGTGC CCTCCTCCAT
    GAATTGAATA AACACCAAAG CTGTGTACGA TGCTGTCGGT TTTCATGGGA TAACCAGTAT
    TTGGCAACCG GTGATGACAG TGGTAAAATT ATGATTTGGA GTGTGCAGAA TGGAGAGCTG
    CTAAGAGAGT GTTGTGACGT ATCAGTGAAT AATGAAAACA GTCTGCATGA TGGTTGGGTG
    ACAGATCTAC ACTTCTCTCC TGACAACAAG CTAATTGTAT CCTCTGGGGC AAATATCAAG
    TGGTGGGAAG TGGACACTGG AGCCTGCAAA CAGACATTCT ACACAATTGG CACAAATCTC
    AAATCCATCC ATGTATGTTC TATATTTAGA ACCTTTGTAA CAATTGACAA TCTGGGAATT
    CTGTATGTGC TGCAGCTAAT AGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31035
    Clone ID Related Accession (Same CDS sequence) XM_012959843.2
    Accession Version XM_012959843.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3747bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product apoptotic protease-activating factor 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959843.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATGAGA AGGCTCGGAG TCTGCTGCTT CAGAATCGAA CAGCACTGGT GAGGGACATT 
    CGCACAGCTT ACATTATGGA TCATATGATA AGTGATCAGG TTATTACCCC AGAGGAGGAG
    GCCCAAGTAA AATCTCAGCG TACTCAGATG GACAAAGCAA ATTTCCTTAT CAATTTAATT
    CTTGGCAAAA GCAATCAGGC ATATGTTTCT TTCTATAATG CTTTGCTTCA TGAGGGGTAC
    AAAGACCTTG CTGTTCTTCT TAAAGAGGCC GCTAATGCTG AGCTTTCTGA CAGTACCAAG
    AGTTCAAGCA ATGGAATTAC TTCATATGTT AAAACAGTAC TATGTGAGGG TGCTGTACCT
    CAGAGACCAG TTGTTTTTGT CACAAGGCTG AAGCTTGTCA GTAAGATACA ACAGAGCCTG
    TATAAGTTGA GTGTGGAATC CGGATGGGTA ACCGTGTATG GAATGGCAGG CTGCGGAAAA
    ACTGTCCTGG CTGCAGAAGC ACTTCGAGAC CACAAAATAC TGAATGAATG TTTTCCTGGA
    GGAGTCCACT GGGTCTCTGT TGGAAGGCAA GACAAAGCCG GGCTCCTGAT GAAACTACAA
    AATTTGTGCA CCCGCCTAGA TCAAGAGGCC AAGTATTCAC AGAGACCTCC ATTAAATGCG
    GAAGAAGCCA GAGAGAGACT GAGGCTGATG GTGAACCGTA TGTACCCTAG GTGCCTCATT
    GTTCTGGATG ATGTTTGGGA TTCTTGGGTG CTCAAAGCTT TTGATATTCA GTGTCGGGTG
    CTCATTACCA GCAGAGACAA GAGTGTGACT GATTCACTGC CTGGCTTTAA GGAGGCTGTT
    CATGTAGACA GTGAGCTTGA GCATTCAAAA GGCCTTGAAA TACTCTCACG CTTCCTGAAT
    ATAAAAGCAG AAGCACTGCC ATCCCAGGCC CATAACATAA TTAAAGTGTC CAAAGGTTCT
    CCTCTTGTAG TATCTCTGAT TGGAGCTCTG CTACGGGAAT TTCCCACCCG CTGGGATTTC
    TACCTCACTC AGCTAAAGAG AAAGCAATTT AAAAGAATAA GGAAGTCTTC CTCCTATGAT
    TACGAGGCTC TCGACGAAGC AATGTCAATA AGTGTGGATA ATCTAAAAGA AGACTTTAAA
    GAGTACTACA ATGATTTCTC CATTATAGAA AAGGATGTGA AAGTTCCCAC ACAGGTTCTG
    TGTATCTTAT GGGACATGGA ACGTGAAGAT GTGGAAGACA TGATGCAGGA GTTTGTCAAC
    AAGTCCTTAG TATACTGTGA TCGTAATGGA AAGTCCTATT CATACTATTT ACATGATCTC
    CAACTGGATT ATCTTACAGA GAGGAATCGG GACCGACTGA CTGTCCTTCA CTCCAAACTT
    GTTGACCAAT ATAAAAAACA CTATTCAAGT AAATCACCTG CTGTGGATCA AGAGGATTCT
    GTGTATTGGT ATCATTACCT TGCTTACCAC ATGGCCAAAG CCAATATGCA CCAAGATCTT
    TGCTCACTCT TGTTTTCCCT GAATTGGTTA AAAGCTAAAA CAGAATTATT TGGGACAGCC
    CACCTCATTC ATGAGTTTGT ACAGTATCGG AATATTCTTG ATAATCAGAA TGCAAAAGCC
    CGAGAACATT TTCAAGAATT TATTGCTGTG AATGGTCATC TTCTTGGGTC GAATCCGGAC
    GTTAGTATTG TGCAGCTGGG CCTTGGTCAG GCTGAAGACT CAGAAGTCTA CAAGCAAGCT
    ATTCTGCAAG CCAAAGAAGA ACTCCAATCT GGAGCGCTCT ATGTCAACTG GATAAACAAG
    CAAAGTATAA AGAACCTGCC AACTTTAGTT GTTCGTCCAC ATAAAGATGC TGTTTTCCAT
    GCATGCTTTT CTTCTGATGG ACAAAGAATT GCTTCTTGTG GAGCTGATAA AACTTTACAG
    GTGTTCAAGA GTGAAACAGG GGAAAAACTC CTTGAAATAG AAGCACATGA TGATGAAGTG
    CTGTGTTGTG CATTTTCTGC AGATGAAAAG CTTCTTGCCA CCTGTTCGGC AGACAGGAAA
    GTAAAGATTT GGAATGCCAA GACAGGAAAA CCCTTAAGAG TGTATGAAGA GCATATAGAA
    CAAGTCAACT GCTGCCAGTT CACCAACAGC CTCTCTGCAC CACTGCTTGC CACATGCTCC
    AATGACTGCT TAATCAAGCT GTGGAATTCT GAGAAGAACT ATTGCCATAA CACTCTTATT
    GGGCACATAG AATCTGTCTA CCATTGCAGA TATTCACCAG ATGATCGGTA TTTAGCCAGT
    TCCTCTATGG ATGGCTCATT AAAGATTTGG GATGTAGAGT CTGCAAATGA GGAGAAGTCA
    ATTGAAGTTG CAAAGCTTTT TGAAAATGAA GATGAAGCGC AGCCTGAAGT ACTACTTAAA
    TGTTGCGCTT GGTCAAATGA TGGTTCAAGG ATTATGGTAA CAGCTCGCAA TTTCCTGTGT
    ATTTATGATG CTGAGAGATG CAACCTTTTG TCTCAACTCA AGGCCTGTCA TCAGATCCTT
    TACTGTGACT TCTGCACCAC TAATCAGATT GCAGCCCTTG CTTTGTCACA CTACGTAGTT
    CAGCTGTGGG ACATAGATTC CTCTACAAAG ATAGCTGACT TCAATGCACA CCTGAGCTGG
    GTTCACTGTG TGAAATTTTC TCCAAAGAGT TCTTCATTCC TGACATCTTC TGATGACCAG
    ACTGTGAAGC TTTGGGAAAC TAGTAATGTC AGCAAACCAG CAGCCACTAA CTTGAAGCGT
    GAGTTTGATG TGACCTTCAG TGGTGAGAGA ACACTGGTGC TTGCAACAAG CAAAGATGAT
    TGTATACTAC TAATTAATGG AATGACTGGA GAGACCTTAT CACAGATCAG AGCACAGGAC
    AATTGTGTTA CGTGCTGCTG TCTCAGTACT GACTATCAGC TAGCAGCTAT TGGGCATAGA
    GATGGAAAAG TAAAGATTTT AGATTTATCC GGCGGTGAAA TCCTTCATAA ACTGGATGGT
    CACAGAGACA CAGTTCAGCA TTGCCAGTTC ACTGCAGATG GAAAACTCCT TGTTTCAAGC
    TCTGATGATT GCACTGTTCG AGTCTGGAAC TTGGTATCTG GAAAGAACTT AGAATTATGT
    GGACACAAAG AACCTGTAAA ATTCTTCAAG ATTTTACAAG AGTCAAAAGT CTTTTCTTGG
    TCTTTTGATG GAACAGTCAA GGTATGGAAT CTCCTCACTG GGCAGCTCAT AAAAGAGTTT
    ATTTGCCATT CTGCAACAGT TCTGGCTTGT GATATCTCAC CTGACTCCAC CAAATTTTCA
    TCTGCCTCTA CTGATAAAAG TGCCAAGATA TGGAACTTAG ATAACTGTGC CCTCCTCCAT
    GAATTGAATA AACACCAAAG CTGTGTACGA TGCTGTCGGT TTTCATGGGA TAACCAGTAT
    TTGGCAACCG GTGATGACAG TGGTAAAATT ATGATTTGGA GTGTGCAGAA TGGAGAGCTG
    CTAAGAGAGT GTTGTGACGT ATCAGTGAAT AATGAAAACA GTCTGCATGA TGGTTGGGTG
    ACAGATCTAC ACTTCTCTCC TGACAACAAG CTAATTGTAT CCTCTGGGGC AAATATCAAG
    TGGTGGGAAG TGGACACTGG AGCCTGCAAA CAGACATTCT ACACAATTGG CACAAATCTC
    AAATCCATCC ATGTATGTTC TATATTTAGA ACCTTTGTAA CAATTGACAA TCTGGGAATT
    CTGTATGTGC TCCAGCTAAT AGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815297.1
    CDS769..4515
    Translation

    Target ORF information:

    RefSeq Version XM_012959843.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis apoptotic peptidase activating factor 1 (apaf1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959843.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATGAGA AGGCTCGGAG TCTGCTGCTT CAGAATCGAA CAGCACTGGT GAGGGACATT 
    CGCACAGCTT ACATTATGGA TCATATGATA AGTGATCAGG TTATTACCCC AGAGGAGGAG
    GCCCAAGTAA AATCTCAGCG TACTCAGATG GACAAAGCAA ATTTCCTTAT CAATTTAATT
    CTTGGCAAAA GCAATCAGGC ATATGTTTCT TTCTATAATG CTTTGCTTCA TGAGGGGTAC
    AAAGACCTTG CTGTTCTTCT TAAAGAGGCC GCTAATGCTG AGCTTTCTGA CAGTACCAAG
    AGTTCAAGCA ATGGAATTAC TTCATATGTT AAAACAGTAC TATGTGAGGG TGCTGTACCT
    CAGAGACCAG TTGTTTTTGT CACAAGGCTG AAGCTTGTCA GTAAGATACA ACAGAGCCTG
    TATAAGTTGA GTGTGGAATC CGGATGGGTA ACCGTGTATG GAATGGCAGG CTGCGGAAAA
    ACTGTCCTGG CTGCAGAAGC ACTTCGAGAC CACAAAATAC TGAATGAATG TTTTCCTGGA
    GGAGTCCACT GGGTCTCTGT TGGAAGGCAA GACAAAGCCG GGCTCCTGAT GAAACTACAA
    AATTTGTGCA CCCGCCTAGA TCAAGAGGCC AAGTATTCAC AGAGACCTCC ATTAAATGCG
    GAAGAAGCCA GAGAGAGACT GAGGCTGATG GTGAACCGTA TGTACCCTAG GTGCCTCATT
    GTTCTGGATG ATGTTTGGGA TTCTTGGGTG CTCAAAGCTT TTGATATTCA GTGTCGGGTG
    CTCATTACCA GCAGAGACAA GAGTGTGACT GATTCACTGC CTGGCTTTAA GGAGGCTGTT
    CATGTAGACA GTGAGCTTGA GCATTCAAAA GGCCTTGAAA TACTCTCACG CTTCCTGAAT
    ATAAAAGCAG AAGCACTGCC ATCCCAGGCC CATAACATAA TTAAAGTGTC CAAAGGTTCT
    CCTCTTGTAG TATCTCTGAT TGGAGCTCTG CTACGGGAAT TTCCCACCCG CTGGGATTTC
    TACCTCACTC AGCTAAAGAG AAAGCAATTT AAAAGAATAA GGAAGTCTTC CTCCTATGAT
    TACGAGGCTC TCGACGAAGC AATGTCAATA AGTGTGGATA ATCTAAAAGA AGACTTTAAA
    GAGTACTACA ATGATTTCTC CATTATAGAA AAGGATGTGA AAGTTCCCAC ACAGGTTCTG
    TGTATCTTAT GGGACATGGA ACGTGAAGAT GTGGAAGACA TGATGCAGGA GTTTGTCAAC
    AAGTCCTTAG TATACTGTGA TCGTAATGGA AAGTCCTATT CATACTATTT ACATGATCTC
    CAACTGGATT ATCTTACAGA GAGGAATCGG GACCGACTGA CTGTCCTTCA CTCCAAACTT
    GTTGACCAAT ATAAAAAACA CTATTCAAGT AAATCACCTG CTGTGGATCA AGAGGATTCT
    GTGTATTGGT ATCATTACCT TGCTTACCAC ATGGCCAAAG CCAATATGCA CCAAGATCTT
    TGCTCACTCT TGTTTTCCCT GAATTGGTTA AAAGCTAAAA CAGAATTATT TGGGACAGCC
    CACCTCATTC ATGAGTTTGT ACAGTATCGG AATATTCTTG ATAATCAGAA TGCAAAAGCC
    CGAGAACATT TTCAAGAATT TATTGCTGTG AATGGTCATC TTCTTGGGTC GAATCCGGAC
    GTTAGTATTG TGCAGCTGGG CCTTGGTCAG GCTGAAGACT CAGAAGTCTA CAAGCAAGCT
    ATTCTGCAAG CCAAAGAAGA ACTCCAATCT GGAGCGCTCT ATGTCAACTG GATAAACAAG
    CAAAGTATAA AGAACCTGCC AACTTTAGTT GTTCGTCCAC ATAAAGATGC TGTTTTCCAT
    GCATGCTTTT CTTCTGATGG ACAAAGAATT GCTTCTTGTG GAGCTGATAA AACTTTACAG
    GTGTTCAAGA GTGAAACAGG GGAAAAACTC CTTGAAATAG AAGCACATGA TGATGAAGTG
    CTGTGTTGTG CATTTTCTGC AGATGAAAAG CTTCTTGCCA CCTGTTCGGC AGACAGGAAA
    GTAAAGATTT GGAATGCCAA GACAGGAAAA CCCTTAAGAG TGTATGAAGA GCATATAGAA
    CAAGTCAACT GCTGCCAGTT CACCAACAGC CTCTCTGCAC CACTGCTTGC CACATGCTCC
    AATGACTGCT TAATCAAGCT GTGGAATTCT GAGAAGAACT ATTGCCATAA CACTCTTATT
    GGGCACATAG AATCTGTCTA CCATTGCAGA TATTCACCAG ATGATCGGTA TTTAGCCAGT
    TCCTCTATGG ATGGCTCATT AAAGATTTGG GATGTAGAGT CTGCAAATGA GGAGAAGTCA
    ATTGAAGTTG CAAAGCTTTT TGAAAATGAA GATGAAGCGC AGCCTGAAGT ACTACTTAAA
    TGTTGCGCTT GGTCAAATGA TGGTTCAAGG ATTATGGTAA CAGCTCGCAA TTTCCTGTGT
    ATTTATGATG CTGAGAGATG CAACCTTTTG TCTCAACTCA AGGCCTGTCA TCAGATCCTT
    TACTGTGACT TCTGCACCAC TAATCAGATT GCAGCCCTTG CTTTGTCACA CTACGTAGTT
    CAGCTGTGGG ACATAGATTC CTCTACAAAG ATAGCTGACT TCAATGCACA CCTGAGCTGG
    GTTCACTGTG TGAAATTTTC TCCAAAGAGT TCTTCATTCC TGACATCTTC TGATGACCAG
    ACTGTGAAGC TTTGGGAAAC TAGTAATGTC AGCAAACCAG CAGCCACTAA CTTGAAGCGT
    GAGTTTGATG TGACCTTCAG TGGTGAGAGA ACACTGGTGC TTGCAACAAG CAAAGATGAT
    TGTATACTAC TAATTAATGG AATGACTGGA GAGACCTTAT CACAGATCAG AGCACAGGAC
    AATTGTGTTA CGTGCTGCTG TCTCAGTACT GACTATCAGC TAGCAGCTAT TGGGCATAGA
    GATGGAAAAG TAAAGATTTT AGATTTATCC GGCGGTGAAA TCCTTCATAA ACTGGATGGT
    CACAGAGACA CAGTTCAGCA TTGCCAGTTC ACTGCAGATG GAAAACTCCT TGTTTCAAGC
    TCTGATGATT GCACTGTTCG AGTCTGGAAC TTGGTATCTG GAAAGAACTT AGAATTATGT
    GGACACAAAG AACCTGTAAA ATTCTTCAAG ATTTTACAAG AGTCAAAAGT CTTTTCTTGG
    TCTTTTGATG GAACAGTCAA GGTATGGAAT CTCCTCACTG GGCAGCTCAT AAAAGAGTTT
    ATTTGCCATT CTGCAACAGT TCTGGCTTGT GATATCTCAC CTGACTCCAC CAAATTTTCA
    TCTGCCTCTA CTGATAAAAG TGCCAAGATA TGGAACTTAG ATAACTGTGC CCTCCTCCAT
    GAATTGAATA AACACCAAAG CTGTGTACGA TGCTGTCGGT TTTCATGGGA TAACCAGTAT
    TTGGCAACCG GTGATGACAG TGGTAAAATT ATGATTTGGA GTGTGCAGAA TGGAGAGCTG
    CTAAGAGAGT GTTGTGACGT ATCAGTGAAT AATGAAAACA GTCTGCATGA TGGTTGGGTG
    ACAGATCTAC ACTTCTCTCC TGACAACAAG CTAATTGTAT CCTCTGGGGC AAATATCAAG
    TGGTGGGAAG TGGACACTGG AGCCTGCAAA CAGACATTCT ACACAATTGG CACAAATCTC
    AAATCCATCC ATGTATGTTC TATATTTAGA ACCTTTGTAA CAATTGACAA TCTGGGAATT
    CTGTATGTGC TCCAGCTAAT AGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.