piwil2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following piwil2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the piwil2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa01958 NM_001112999.1
Latest version!
Xenopus tropicalis piwi-like RNA-mediated gene silencing 2 (piwil2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa30962 XM_012958498.3
Latest version!
Xenopus tropicalis piwi-like RNA-mediated gene silencing 2 (piwil2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa43020 XM_018091922.1
Latest version!
Xenopus tropicalis piwi-like RNA-mediated gene silencing 2 (piwil2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
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OXa30962 XM_012958498.2 Xenopus tropicalis piwi-like RNA-mediated gene silencing 2 (piwil2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa01958
    Clone ID Related Accession (Same CDS sequence) NM_001112999.1
    Accession Version NM_001112999.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2850bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-24
    Organism Xenopus tropicalis(tropical clawed frog)
    Product piwi-like protein 2
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC154090.1. ##Evidence-Data-START## Transcript exon combination :: BC154090.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028290, SAMD00028291 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGATCCTA CCAGACCTCC ATTCAGGGGT TCCCCCTTTC ATACACCATT GGGAGTGCGT 
    CCTCCAGTTC TAGAAACTAA GGAAGAAGGG CCTCATGGAA GAGCAGTCCT ATTACCACGG
    GGCCGTGCAT TATTGGGTGC ATCAGCACCT TCTTCTGATA CTACCCAAAG AGATCCATCT
    GACTCTAGGA ATGTTCTCCC TGCTCTATTT CGTGGTATGG GAATTGAAAC AAAGCCCAGT
    GGGATTCCTG GCCGTGGTGG GCCAGTCTTT GGCAGGGGGT TTCTTTCTAC TATGTCTTCA
    GGAGATGGCC CTGATCAGCC ACTTTCAGAA CCCTCTATAA GACCCTCCCT CTCAACCCGT
    GTTCAGCAAG CAAGTGATTT TAGCACAGAG AGGGTTGCAC TTGGGAGAGC CAGGTTTCCA
    ATACCTCCGG TGAGTATGGA AAAGCCACAT GTACCCACTG GAAGGGGTTT GTTATTTCCC
    TCTGTTCTAC CCACTTTGAC ATCTGACCCA GTACCTAAAG AAAGCCCTAT CGCTACACTT
    CAAATTGAGA AGGAAGAGAA ATGGGAGCCT TTACCAAAGA AAGGCAGCAA AGGTAGCCCC
    TGTCAGCTTG GACTGAACCT CATTAAAATT AACTTCCAGA ATGAAGCCGT ATATCAGTAC
    CATGTCACAT TTACTCCCAT AGTGGAGTGC CGTAGTATGC GTTTTGGTAT GATGAAGGAC
    CACCGCTCTG TGACAGGACC AGTAACTGCA TTCGACGGTT CTATACTTTA CCTTCCTGTC
    AAACTTGCAC AGACAGTTGA GCTGGAGAGT GAACGCCGGA CAGATGGACA AAAAATAAAG
    ATAACCATAC AGATGACAAA GATTCTGGAT CCCAGTTCAG ACTTGTGCCT TCCTTTTTAT
    AATGTAGTCA TGAGGAGGGT GTTCAAAATT CTGGACCTGA AACTTGTTGG GAGAAATTTT
    TATGACCCAG CAAGTTCAAC AGTGCTGCAG CAGTACAGAC TGCAGGTGTG GCCAGGCTAT
    GCTGCAAATA TACGCAAAAC TGACGGTGGG CTATTTCTTT TGGTTGACAT CACCCACAAG
    ATTATCCGCA GTGATTCTGT GCTGGACATC ATGAACATTC TATATCAGCA GAGCCCTGAG
    AACTTTCAGG ATGAGGTTAC CAAGCAGCTG GTTGGCAGCA TTGTGATCAC CAGATACAAC
    AACCGTACCT ACCGAATAGA TGACATTGAA TGGAACATGA GCCCCAAGGA CTCCTTCTCT
    ATGTCAGATG GAAGCAAAAT CAGTTTCATT GATTATTACA GCAAGAACTA TGGCATAACT
    GTTAAGGAGC TGGACCAACC CTTGCTGCTT CACAGACCTA GTGAAAGGAA GGCACCAAAG
    GGCAAGGCAC TTGATATTGT CCTCCTCTTA CCAGAACTTG CTTTTATGAC TGGCATCCCT
    GAAAAAATGA GAAAAGACTT TCGGGCCATG AAGGATCTGA CACAGCAGAT TCATTTAAGC
    CCAAAACAGC ATCATATCTC TTTGGGGAAA TTGCTAAAAC GTATTGAAAG CAGTGCGGAT
    GCAAAGAATG AACTGCAGCG TTGGGGCTTA TTCCTAGACA CTGATATTCA CATGACCACT
    GGTAGGATTT TACCAATAGA GAAAATAAAT CTGCGTAATA ACTCATTTCC TGCAGGAGAA
    GATCTCAATT GGAACAGGGA AGTGACACGT GAGGCATGCC GTTCCTCAGT GCATCTTCTT
    TATTGGGCAA TGATATATCC TAAACGGGCT TCAGCACAGG CACAAGAATT GTCTAGTATG
    CTGGAGAGGA TTGGAGGACC TATTGGAATA CGAGTAAACC ATCCTAACTG TGTGGAGCTC
    CGGGATGACC GTGTCGAAAC GTACGCGCGC AGCATCAAAT CACTGTTAGA GGGTGAGGGC
    AAGGTTCAGC TACTAGTGTG TCTTATATCT GGGACCAGAG ATGACCTGTA CGGTGCTATT
    AAGAAATTGT GTTGTGTGCA GAACCCAGTT CCATCACAGG TGATTAATAC CAGAACCATC
    TCCCAGCCCC AGAAGCTCAG GAGCATTGCA CAGAAAATTC TGCTTCAGAT TAACTGCAAG
    CTGGGAGGGG AACTGTGGGG AGTAGACATC CCTCTGAAAA GTGTGATGGT GATTGGGATG
    GATGTTTATC ACGACCCCAG CAGAGGCATG CGCTCTGTAT TAGGCTTTGT TGCTAGCATC
    AACAGCTGCC TGACTGCTTG GTATTCTCGT GTGGTCTTTC AGCTTCCTAA CCAGGAGATC
    ATGGATAGCC TAAAGCTTTG CCTTGTGGCA GCCCTGCAGA AATTCTTTGA GGTGAATCAC
    AGTCTTCCAG AGAAGATTGT GGTGTATCGA GATGGCGTAT CTGATGGGCA ACTTAACACG
    GTGGAAAACT ATGAGATCCC ACAGCTTCAA ACTTGCTTCC AGACATTTGA TAACTATAAT
    CCACGTATGG TGGTTATTGT AGTACAGAAA AGGGTTAGCA CTAATCTGTA CTCTACCGCC
    ACTGGGCAGT TTCTTACCCC TCAACCTGGG ACTGTTATAG ACCATACAGT CACCAACCGC
    AAGTGGATAG ATTTCTTCCT GATGTCTCAT CATGTGAGAC AAGGCTGCGG GATCCCCACC
    CACTACATTT GTGTCATGAA TACAGCAAAT TTGGGTCCTG ACCATTTACA GAGGTTGACC
    TTCAAGCTTT GCCACATGTA CTGGAACTGG CCTGGGACCA TCCGAGTGCC AGCTCCATGT
    AAATACGCAC ACAAGCTGGC CTTCCTTTCC GGTCAGTTTC TGCACCACGA GCCTTCCATA
    AAGCTGTGTG ACAAGCTTTT CTTCCTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001106470.1
    CDS77..2926
    Translation

    Target ORF information:

    RefSeq Version NM_001112999.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis piwi-like RNA-mediated gene silencing 2 (piwil2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001112999.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGATCCTA CCAGACCTCC ATTCAGGGGT TCCCCCTTTC ATACACCATT GGGAGTGCGT 
    CCTCCAGTTC TAGAAACTAA GGAAGAAGGG CCTCATGGAA GAGCAGTCCT ATTACCACGG
    GGCCGTGCAT TATTGGGTGC ATCAGCACCT TCTTCTGATA CTACCCAAAG AGATCCATCT
    GACTCTAGGA ATGTTCTCCC TGCTCTATTT CGTGGTATGG GAATTGAAAC AAAGCCCAGT
    GGGATTCCTG GCCGTGGTGG GCCAGTCTTT GGCAGGGGGT TTCTTTCTAC TATGTCTTCA
    GGAGATGGCC CTGATCAGCC ACTTTCAGAA CCCTCTATAA GACCCTCCCT CTCAACCCGT
    GTTCAGCAAG CAAGTGATTT TAGCACAGAG AGGGTTGCAC TTGGGAGAGC CAGGTTTCCA
    ATACCTCCGG TGAGTATGGA AAAGCCACAT GTACCCACTG GAAGGGGTTT GTTATTTCCC
    TCTGTTCTAC CCACTTTGAC ATCTGACCCA GTACCTAAAG AAAGCCCTAT CGCTACACTT
    CAAATTGAGA AGGAAGAGAA ATGGGAGCCT TTACCAAAGA AAGGCAGCAA AGGTAGCCCC
    TGTCAGCTTG GACTGAACCT CATTAAAATT AACTTCCAGA ATGAAGCCGT ATATCAGTAC
    CATGTCACAT TTACTCCCAT AGTGGAGTGC CGTAGTATGC GTTTTGGTAT GATGAAGGAC
    CACCGCTCTG TGACAGGACC AGTAACTGCA TTCGACGGTT CTATACTTTA CCTTCCTGTC
    AAACTTGCAC AGACAGTTGA GCTGGAGAGT GAACGCCGGA CAGATGGACA AAAAATAAAG
    ATAACCATAC AGATGACAAA GATTCTGGAT CCCAGTTCAG ACTTGTGCCT TCCTTTTTAT
    AATGTAGTCA TGAGGAGGGT GTTCAAAATT CTGGACCTGA AACTTGTTGG GAGAAATTTT
    TATGACCCAG CAAGTTCAAC AGTGCTGCAG CAGTACAGAC TGCAGGTGTG GCCAGGCTAT
    GCTGCAAATA TACGCAAAAC TGACGGTGGG CTATTTCTTT TGGTTGACAT CACCCACAAG
    ATTATCCGCA GTGATTCTGT GCTGGACATC ATGAACATTC TATATCAGCA GAGCCCTGAG
    AACTTTCAGG ATGAGGTTAC CAAGCAGCTG GTTGGCAGCA TTGTGATCAC CAGATACAAC
    AACCGTACCT ACCGAATAGA TGACATTGAA TGGAACATGA GCCCCAAGGA CTCCTTCTCT
    ATGTCAGATG GAAGCAAAAT CAGTTTCATT GATTATTACA GCAAGAACTA TGGCATAACT
    GTTAAGGAGC TGGACCAACC CTTGCTGCTT CACAGACCTA GTGAAAGGAA GGCACCAAAG
    GGCAAGGCAC TTGATATTGT CCTCCTCTTA CCAGAACTTG CTTTTATGAC TGGCATCCCT
    GAAAAAATGA GAAAAGACTT TCGGGCCATG AAGGATCTGA CACAGCAGAT TCATTTAAGC
    CCAAAACAGC ATCATATCTC TTTGGGGAAA TTGCTAAAAC GTATTGAAAG CAGTGCGGAT
    GCAAAGAATG AACTGCAGCG TTGGGGCTTA TTCCTAGACA CTGATATTCA CATGACCACT
    GGTAGGATTT TACCAATAGA GAAAATAAAT CTGCGTAATA ACTCATTTCC TGCAGGAGAA
    GATCTCAATT GGAACAGGGA AGTGACACGT GAGGCATGCC GTTCCTCAGT GCATCTTCTT
    TATTGGGCAA TGATATATCC TAAACGGGCT TCAGCACAGG CACAAGAATT GTCTAGTATG
    CTGGAGAGGA TTGGAGGACC TATTGGAATA CGAGTAAACC ATCCTAACTG TGTGGAGCTC
    CGGGATGACC GTGTCGAAAC GTACGCGCGC AGCATCAAAT CACTGTTAGA GGGTGAGGGC
    AAGGTTCAGC TACTAGTGTG TCTTATATCT GGGACCAGAG ATGACCTGTA CGGTGCTATT
    AAGAAATTGT GTTGTGTGCA GAACCCAGTT CCATCACAGG TGATTAATAC CAGAACCATC
    TCCCAGCCCC AGAAGCTCAG GAGCATTGCA CAGAAAATTC TGCTTCAGAT TAACTGCAAG
    CTGGGAGGGG AACTGTGGGG AGTAGACATC CCTCTGAAAA GTGTGATGGT GATTGGGATG
    GATGTTTATC ACGACCCCAG CAGAGGCATG CGCTCTGTAT TAGGCTTTGT TGCTAGCATC
    AACAGCTGCC TGACTGCTTG GTATTCTCGT GTGGTCTTTC AGCTTCCTAA CCAGGAGATC
    ATGGATAGCC TAAAGCTTTG CCTTGTGGCA GCCCTGCAGA AATTCTTTGA GGTGAATCAC
    AGTCTTCCAG AGAAGATTGT GGTGTATCGA GATGGCGTAT CTGATGGGCA ACTTAACACG
    GTGGAAAACT ATGAGATCCC ACAGCTTCAA ACTTGCTTCC AGACATTTGA TAACTATAAT
    CCACGTATGG TGGTTATTGT AGTACAGAAA AGGGTTAGCA CTAATCTGTA CTCTACCGCC
    ACTGGGCAGT TTCTTACCCC TCAACCTGGG ACTGTTATAG ACCATACAGT CACCAACCGC
    AAGTGGATAG ATTTCTTCCT GATGTCTCAT CATGTGAGAC AAGGCTGCGG GATCCCCACC
    CACTACATTT GTGTCATGAA TACAGCAAAT TTGGGTCCTG ACCATTTACA GAGGTTGACC
    TTCAAGCTTT GCCACATGTA CTGGAACTGG CCTGGGACCA TCCGAGTGCC AGCTCCATGT
    AAATACGCAC ACAAGCTGGC CTTCCTTTCC GGTCAGTTTC TGCACCACGA GCCTTCCATA
    AAGCTGTGTG ACAAGCTTTT CTTCCTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30962
    Clone ID Related Accession (Same CDS sequence) XM_012958498.3 , XM_012958498.2
    Accession Version XM_012958498.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2883bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product piwi-like protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012958498.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGGAAGTC AGCAGCGGGT TACCGCCCAA AAAATGGATC CTACCAGACC TCCATTCAGG 
    GGTTCCCCCT TTCATACACC ATTGGGAGTG CGTCCTCCAG TTCTAGAAAC TAAAGAAGAA
    GGGCCACATG GAAGAGCAGT CCTATTACCA CGGGGCCGTG CATTATTGGG TGCATCAGCA
    CCTTCTTCTG ATACTACCCA AAGAGATCCA TCTGACTCTA GGAATGTTCT CCCTGCTCTA
    TTTCGTGGTA TGGGAATTGA AACAAAGCCC AGTGGGATTC CTGGCCGTGG TGGGCCAGTC
    TTTGGCAGGG GGTTTCTTTC TACTATGTCT TCAGGAGATG GCCCTGATCA GCCACTTTCA
    GAACCCTCTA TAAGACCCTC CCTCTCAACC CGTGTTCAGC AAGCAAGTGA TTTTAGCACA
    GAGAGGGTTG CACTTGGGAG AGCCAGGTTT CCAATACCTC CGGTGAGTAT GGAAAAGCCA
    CATGTACCCA CTGGAAGGGG TTTGTTATTT CCCTCTGTTC TACCCACTTT GACATCTGAC
    CCAGTACCTA AAGAAAGCCC TATCGCTACA CTTCAAATTG AGAAGGAAGA GAAATGGGAG
    CCTTTACCAA AGAAAGGCAG CAAAGGTAGC CCCTGTCAGC TTGGACTGAA CCTCATTAAA
    ATTAACTTCC AGAATGAAGC CGTATATCAG TACCATGTCA CATTTACTCC CATAGTGGAG
    TGCCGTAGTA TGCGTTTTGG TATGATGAAG GACCACCGCT CTGTGACAGG ACCAGTAACT
    GCATTCGACG GTTCTATACT TTACCTTCCT GTCAAACTTG CACAGACAGT TGAGCTGGAG
    AGTGAACGCC GGACAGATGG ACAAAAAATA AAGATAACCA TACAGATGAC AAAGATTCTG
    GATCCCAGTT CAGACTTGTG CCTTCCTTTT TATAATGTAG TCATGAGGAG GGTGTTCAAA
    ATTCTGGACC TGAAACTTGT TGGGAGAAAT TTTTATGACC CAGCAAGTTC AACAGTGCTG
    CAGCAGTACA GACTGCAGGT GTGGCCAGGC TATGCTGCAA ATATACGCAA AACTGACGGT
    GGGCTATTTC TTTTGGTTGA CATCACCCAC AAGATTATCC GCAGTGATTC TGTGCTGGAC
    ATCATGAACA TTCTATATCA GCAGAGCCCT GAGAACTTTC AGGATGAGGT TACCAAGCAG
    CTGGTTGGCA GCATTGTGAT CACCAGATAC AACAACCGTA CCTACCGAAT AGATGACATT
    GAATGGAACA TGAGCCCCAA GGACTCCTTC TCTATGTCAG ATGGAAGCAA AGTCAGTTTC
    ATTGATTATT ACAGCAAGAA CTATGGCATA ACTGTTAAGG AGCTGGACCA ACCCTTGCTG
    CTTCACAGAC CTAGTGAAAG GAAGGCACCA AAGGGCAAGG CACTTGATAT TGTCCTCCTC
    TTACCAGAAC TTGCTTTTAT GACTGGCATC CCTGAAAAAA TGAGAAAAGA CTTTCGGGCC
    ATGAAGGATC TGACACAGCA GATTCATTTA AGCCCAAAAC AGCATCATAT CTCTTTGGGG
    AAATTGCTAA AACGTATTGA AAGCAGTGCG GATGCAAAGA ATGAACTGCA GCGTTGGGGC
    TTATTCCTAG ACACTGATAT TCACATGACC ACTGGTAGGA TTTTACCAAT AGAGAAAATA
    AATCTGCGTA ATAACTCATT TCCTGCAGGA GAAGATCTCA ATTGGAACAG GGAAGTGACA
    CGTGAGGCAT GCCGTTCCTC AGTGCATCTT CTTTATTGGG CAATGATATA TCCTAAACGG
    GCTTCAGCAC AGGCACAAGA ATTGTCTAGT ATGCTGGAGA GGATTGGAGG ACCTATTGGA
    ATACGAGTAA ACCATCCTAA CTGTGTGGAG CTCCGGGATG ACCGTGTCGA AACGTACGCG
    CGCAGCATCA AATCACTGTT AGAGGGTGAG GGCAAAGTTC AGCTACTAGT GTGTCTTATA
    TCTGGGACCA GAGATGACCT GTACGGTGCT ATTAAGAAAT TGTGTTGTGT ACAGAACCCA
    GTTCCATCAC AGGTGATTAA TACCAGAACC ATCTCCCAGC CCCAGAAGCT CAGGAGCATT
    GCACAGAAAA TTCTGCTTCA GATTAACTGC AAGCTGGGAG GGGAACTGTG GGGAGTAGAC
    ATCCCTCTGA AAAGTGTGAT GGTGATTGGG ATGGATGTTT ATCACGACCC CAGCAGAGGC
    ATGCGCTCTG TATTAGGCTT TGTTGCTAGC ATCAACAGCT GCCTGACTGC TTGGTATTCT
    CGTGTGGTCT TTCAGCTTCC TAACCAGGAG ATCATGGATA GCCTAAAGCT TTGCCTTGTG
    GCAGCCCTGC AGAAATTCTT TGAGGTGAAT CACAGTCTTC CAGAGAAGAT TGTGGTGTAT
    CGAGATGGCG TATCTGATGG GCAACTTAAC ACGGTGGAAA ACTATGAGAT CCCACAGCTT
    CAAACTTGCT TCCAGACATT TGATAACTAT AATCCACGTA TGGTGGTTAT TGTAGTACAG
    AAAAGGGTTA GCACTAATCT GTACTCTACC GCCACTGGGC AGTTTCTTAC CCCTCAACCT
    GGGACTGTTA TAGACCATAC AGTCACCAAC CGCAAGTGGA TAGATTTCTT CCTGATGTCT
    CATCATGTGA GACAAGGCTG CGGGATCCCC ACCCACTACA TTTGTGTCAT GAATACAGCA
    AATTTGGGTC CTGACCATTT ACAGAGGTTG ACCTTCAAGC TTTGCCACAT GTACTGGAAC
    TGGCCTGGGA CCATCCGAGT GCCAGCTCCA TGTAAATACG CACACAAGCT GGCCTTCCTT
    TCCGGTCAGT TTCTGCACCA CGAGCCTTCC ATAAAGCTGT GTGACAAGCT TTTCTTCCTT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813952.1
    CDS70..2952
    Translation

    Target ORF information:

    RefSeq Version XM_012958498.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis piwi-like RNA-mediated gene silencing 2 (piwil2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012958498.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGGAAGTC AGCAGCGGGT TACCGCCCAA AAAATGGATC CTACCAGACC TCCATTCAGG 
    GGTTCCCCCT TTCATACACC ATTGGGAGTG CGTCCTCCAG TTCTAGAAAC TAAAGAAGAA
    GGGCCACATG GAAGAGCAGT CCTATTACCA CGGGGCCGTG CATTATTGGG TGCATCAGCA
    CCTTCTTCTG ATACTACCCA AAGAGATCCA TCTGACTCTA GGAATGTTCT CCCTGCTCTA
    TTTCGTGGTA TGGGAATTGA AACAAAGCCC AGTGGGATTC CTGGCCGTGG TGGGCCAGTC
    TTTGGCAGGG GGTTTCTTTC TACTATGTCT TCAGGAGATG GCCCTGATCA GCCACTTTCA
    GAACCCTCTA TAAGACCCTC CCTCTCAACC CGTGTTCAGC AAGCAAGTGA TTTTAGCACA
    GAGAGGGTTG CACTTGGGAG AGCCAGGTTT CCAATACCTC CGGTGAGTAT GGAAAAGCCA
    CATGTACCCA CTGGAAGGGG TTTGTTATTT CCCTCTGTTC TACCCACTTT GACATCTGAC
    CCAGTACCTA AAGAAAGCCC TATCGCTACA CTTCAAATTG AGAAGGAAGA GAAATGGGAG
    CCTTTACCAA AGAAAGGCAG CAAAGGTAGC CCCTGTCAGC TTGGACTGAA CCTCATTAAA
    ATTAACTTCC AGAATGAAGC CGTATATCAG TACCATGTCA CATTTACTCC CATAGTGGAG
    TGCCGTAGTA TGCGTTTTGG TATGATGAAG GACCACCGCT CTGTGACAGG ACCAGTAACT
    GCATTCGACG GTTCTATACT TTACCTTCCT GTCAAACTTG CACAGACAGT TGAGCTGGAG
    AGTGAACGCC GGACAGATGG ACAAAAAATA AAGATAACCA TACAGATGAC AAAGATTCTG
    GATCCCAGTT CAGACTTGTG CCTTCCTTTT TATAATGTAG TCATGAGGAG GGTGTTCAAA
    ATTCTGGACC TGAAACTTGT TGGGAGAAAT TTTTATGACC CAGCAAGTTC AACAGTGCTG
    CAGCAGTACA GACTGCAGGT GTGGCCAGGC TATGCTGCAA ATATACGCAA AACTGACGGT
    GGGCTATTTC TTTTGGTTGA CATCACCCAC AAGATTATCC GCAGTGATTC TGTGCTGGAC
    ATCATGAACA TTCTATATCA GCAGAGCCCT GAGAACTTTC AGGATGAGGT TACCAAGCAG
    CTGGTTGGCA GCATTGTGAT CACCAGATAC AACAACCGTA CCTACCGAAT AGATGACATT
    GAATGGAACA TGAGCCCCAA GGACTCCTTC TCTATGTCAG ATGGAAGCAA AGTCAGTTTC
    ATTGATTATT ACAGCAAGAA CTATGGCATA ACTGTTAAGG AGCTGGACCA ACCCTTGCTG
    CTTCACAGAC CTAGTGAAAG GAAGGCACCA AAGGGCAAGG CACTTGATAT TGTCCTCCTC
    TTACCAGAAC TTGCTTTTAT GACTGGCATC CCTGAAAAAA TGAGAAAAGA CTTTCGGGCC
    ATGAAGGATC TGACACAGCA GATTCATTTA AGCCCAAAAC AGCATCATAT CTCTTTGGGG
    AAATTGCTAA AACGTATTGA AAGCAGTGCG GATGCAAAGA ATGAACTGCA GCGTTGGGGC
    TTATTCCTAG ACACTGATAT TCACATGACC ACTGGTAGGA TTTTACCAAT AGAGAAAATA
    AATCTGCGTA ATAACTCATT TCCTGCAGGA GAAGATCTCA ATTGGAACAG GGAAGTGACA
    CGTGAGGCAT GCCGTTCCTC AGTGCATCTT CTTTATTGGG CAATGATATA TCCTAAACGG
    GCTTCAGCAC AGGCACAAGA ATTGTCTAGT ATGCTGGAGA GGATTGGAGG ACCTATTGGA
    ATACGAGTAA ACCATCCTAA CTGTGTGGAG CTCCGGGATG ACCGTGTCGA AACGTACGCG
    CGCAGCATCA AATCACTGTT AGAGGGTGAG GGCAAAGTTC AGCTACTAGT GTGTCTTATA
    TCTGGGACCA GAGATGACCT GTACGGTGCT ATTAAGAAAT TGTGTTGTGT ACAGAACCCA
    GTTCCATCAC AGGTGATTAA TACCAGAACC ATCTCCCAGC CCCAGAAGCT CAGGAGCATT
    GCACAGAAAA TTCTGCTTCA GATTAACTGC AAGCTGGGAG GGGAACTGTG GGGAGTAGAC
    ATCCCTCTGA AAAGTGTGAT GGTGATTGGG ATGGATGTTT ATCACGACCC CAGCAGAGGC
    ATGCGCTCTG TATTAGGCTT TGTTGCTAGC ATCAACAGCT GCCTGACTGC TTGGTATTCT
    CGTGTGGTCT TTCAGCTTCC TAACCAGGAG ATCATGGATA GCCTAAAGCT TTGCCTTGTG
    GCAGCCCTGC AGAAATTCTT TGAGGTGAAT CACAGTCTTC CAGAGAAGAT TGTGGTGTAT
    CGAGATGGCG TATCTGATGG GCAACTTAAC ACGGTGGAAA ACTATGAGAT CCCACAGCTT
    CAAACTTGCT TCCAGACATT TGATAACTAT AATCCACGTA TGGTGGTTAT TGTAGTACAG
    AAAAGGGTTA GCACTAATCT GTACTCTACC GCCACTGGGC AGTTTCTTAC CCCTCAACCT
    GGGACTGTTA TAGACCATAC AGTCACCAAC CGCAAGTGGA TAGATTTCTT CCTGATGTCT
    CATCATGTGA GACAAGGCTG CGGGATCCCC ACCCACTACA TTTGTGTCAT GAATACAGCA
    AATTTGGGTC CTGACCATTT ACAGAGGTTG ACCTTCAAGC TTTGCCACAT GTACTGGAAC
    TGGCCTGGGA CCATCCGAGT GCCAGCTCCA TGTAAATACG CACACAAGCT GGCCTTCCTT
    TCCGGTCAGT TTCTGCACCA CGAGCCTTCC ATAAAGCTGT GTGACAAGCT TTTCTTCCTT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30962
    Clone ID Related Accession (Same CDS sequence) XM_012958498.3 , XM_012958498.2
    Accession Version XM_012958498.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2883bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product piwi-like protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012958498.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGGAAGTC AGCAGCGGGT TACCGCCCAA AAAATGGATC CTACCAGACC TCCATTCAGG 
    GGTTCCCCCT TTCATACACC ATTGGGAGTG CGTCCTCCAG TTCTAGAAAC TAAAGAAGAA
    GGGCCACATG GAAGAGCAGT CCTATTACCA CGGGGCCGTG CATTATTGGG TGCATCAGCA
    CCTTCTTCTG ATACTACCCA AAGAGATCCA TCTGACTCTA GGAATGTTCT CCCTGCTCTA
    TTTCGTGGTA TGGGAATTGA AACAAAGCCC AGTGGGATTC CTGGCCGTGG TGGGCCAGTC
    TTTGGCAGGG GGTTTCTTTC TACTATGTCT TCAGGAGATG GCCCTGATCA GCCACTTTCA
    GAACCCTCTA TAAGACCCTC CCTCTCAACC CGTGTTCAGC AAGCAAGTGA TTTTAGCACA
    GAGAGGGTTG CACTTGGGAG AGCCAGGTTT CCAATACCTC CGGTGAGTAT GGAAAAGCCA
    CATGTACCCA CTGGAAGGGG TTTGTTATTT CCCTCTGTTC TACCCACTTT GACATCTGAC
    CCAGTACCTA AAGAAAGCCC TATCGCTACA CTTCAAATTG AGAAGGAAGA GAAATGGGAG
    CCTTTACCAA AGAAAGGCAG CAAAGGTAGC CCCTGTCAGC TTGGACTGAA CCTCATTAAA
    ATTAACTTCC AGAATGAAGC CGTATATCAG TACCATGTCA CATTTACTCC CATAGTGGAG
    TGCCGTAGTA TGCGTTTTGG TATGATGAAG GACCACCGCT CTGTGACAGG ACCAGTAACT
    GCATTCGACG GTTCTATACT TTACCTTCCT GTCAAACTTG CACAGACAGT TGAGCTGGAG
    AGTGAACGCC GGACAGATGG ACAAAAAATA AAGATAACCA TACAGATGAC AAAGATTCTG
    GATCCCAGTT CAGACTTGTG CCTTCCTTTT TATAATGTAG TCATGAGGAG GGTGTTCAAA
    ATTCTGGACC TGAAACTTGT TGGGAGAAAT TTTTATGACC CAGCAAGTTC AACAGTGCTG
    CAGCAGTACA GACTGCAGGT GTGGCCAGGC TATGCTGCAA ATATACGCAA AACTGACGGT
    GGGCTATTTC TTTTGGTTGA CATCACCCAC AAGATTATCC GCAGTGATTC TGTGCTGGAC
    ATCATGAACA TTCTATATCA GCAGAGCCCT GAGAACTTTC AGGATGAGGT TACCAAGCAG
    CTGGTTGGCA GCATTGTGAT CACCAGATAC AACAACCGTA CCTACCGAAT AGATGACATT
    GAATGGAACA TGAGCCCCAA GGACTCCTTC TCTATGTCAG ATGGAAGCAA AGTCAGTTTC
    ATTGATTATT ACAGCAAGAA CTATGGCATA ACTGTTAAGG AGCTGGACCA ACCCTTGCTG
    CTTCACAGAC CTAGTGAAAG GAAGGCACCA AAGGGCAAGG CACTTGATAT TGTCCTCCTC
    TTACCAGAAC TTGCTTTTAT GACTGGCATC CCTGAAAAAA TGAGAAAAGA CTTTCGGGCC
    ATGAAGGATC TGACACAGCA GATTCATTTA AGCCCAAAAC AGCATCATAT CTCTTTGGGG
    AAATTGCTAA AACGTATTGA AAGCAGTGCG GATGCAAAGA ATGAACTGCA GCGTTGGGGC
    TTATTCCTAG ACACTGATAT TCACATGACC ACTGGTAGGA TTTTACCAAT AGAGAAAATA
    AATCTGCGTA ATAACTCATT TCCTGCAGGA GAAGATCTCA ATTGGAACAG GGAAGTGACA
    CGTGAGGCAT GCCGTTCCTC AGTGCATCTT CTTTATTGGG CAATGATATA TCCTAAACGG
    GCTTCAGCAC AGGCACAAGA ATTGTCTAGT ATGCTGGAGA GGATTGGAGG ACCTATTGGA
    ATACGAGTAA ACCATCCTAA CTGTGTGGAG CTCCGGGATG ACCGTGTCGA AACGTACGCG
    CGCAGCATCA AATCACTGTT AGAGGGTGAG GGCAAAGTTC AGCTACTAGT GTGTCTTATA
    TCTGGGACCA GAGATGACCT GTACGGTGCT ATTAAGAAAT TGTGTTGTGT ACAGAACCCA
    GTTCCATCAC AGGTGATTAA TACCAGAACC ATCTCCCAGC CCCAGAAGCT CAGGAGCATT
    GCACAGAAAA TTCTGCTTCA GATTAACTGC AAGCTGGGAG GGGAACTGTG GGGAGTAGAC
    ATCCCTCTGA AAAGTGTGAT GGTGATTGGG ATGGATGTTT ATCACGACCC CAGCAGAGGC
    ATGCGCTCTG TATTAGGCTT TGTTGCTAGC ATCAACAGCT GCCTGACTGC TTGGTATTCT
    CGTGTGGTCT TTCAGCTTCC TAACCAGGAG ATCATGGATA GCCTAAAGCT TTGCCTTGTG
    GCAGCCCTGC AGAAATTCTT TGAGGTGAAT CACAGTCTTC CAGAGAAGAT TGTGGTGTAT
    CGAGATGGCG TATCTGATGG GCAACTTAAC ACGGTGGAAA ACTATGAGAT CCCACAGCTT
    CAAACTTGCT TCCAGACATT TGATAACTAT AATCCACGTA TGGTGGTTAT TGTAGTACAG
    AAAAGGGTTA GCACTAATCT GTACTCTACC GCCACTGGGC AGTTTCTTAC CCCTCAACCT
    GGGACTGTTA TAGACCATAC AGTCACCAAC CGCAAGTGGA TAGATTTCTT CCTGATGTCT
    CATCATGTGA GACAAGGCTG CGGGATCCCC ACCCACTACA TTTGTGTCAT GAATACAGCA
    AATTTGGGTC CTGACCATTT ACAGAGGTTG ACCTTCAAGC TTTGCCACAT GTACTGGAAC
    TGGCCTGGGA CCATCCGAGT GCCAGCTCCA TGTAAATACG CACACAAGCT GGCCTTCCTT
    TCCGGTCAGT TTCTGCACCA CGAGCCTTCC ATAAAGCTGT GTGACAAGCT TTTCTTCCTT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813952.1
    CDS58..2940
    Translation

    Target ORF information:

    RefSeq Version XM_012958498.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis piwi-like RNA-mediated gene silencing 2 (piwil2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012958498.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGGAAGTC AGCAGCGGGT TACCGCCCAA AAAATGGATC CTACCAGACC TCCATTCAGG 
    GGTTCCCCCT TTCATACACC ATTGGGAGTG CGTCCTCCAG TTCTAGAAAC TAAAGAAGAA
    GGGCCACATG GAAGAGCAGT CCTATTACCA CGGGGCCGTG CATTATTGGG TGCATCAGCA
    CCTTCTTCTG ATACTACCCA AAGAGATCCA TCTGACTCTA GGAATGTTCT CCCTGCTCTA
    TTTCGTGGTA TGGGAATTGA AACAAAGCCC AGTGGGATTC CTGGCCGTGG TGGGCCAGTC
    TTTGGCAGGG GGTTTCTTTC TACTATGTCT TCAGGAGATG GCCCTGATCA GCCACTTTCA
    GAACCCTCTA TAAGACCCTC CCTCTCAACC CGTGTTCAGC AAGCAAGTGA TTTTAGCACA
    GAGAGGGTTG CACTTGGGAG AGCCAGGTTT CCAATACCTC CGGTGAGTAT GGAAAAGCCA
    CATGTACCCA CTGGAAGGGG TTTGTTATTT CCCTCTGTTC TACCCACTTT GACATCTGAC
    CCAGTACCTA AAGAAAGCCC TATCGCTACA CTTCAAATTG AGAAGGAAGA GAAATGGGAG
    CCTTTACCAA AGAAAGGCAG CAAAGGTAGC CCCTGTCAGC TTGGACTGAA CCTCATTAAA
    ATTAACTTCC AGAATGAAGC CGTATATCAG TACCATGTCA CATTTACTCC CATAGTGGAG
    TGCCGTAGTA TGCGTTTTGG TATGATGAAG GACCACCGCT CTGTGACAGG ACCAGTAACT
    GCATTCGACG GTTCTATACT TTACCTTCCT GTCAAACTTG CACAGACAGT TGAGCTGGAG
    AGTGAACGCC GGACAGATGG ACAAAAAATA AAGATAACCA TACAGATGAC AAAGATTCTG
    GATCCCAGTT CAGACTTGTG CCTTCCTTTT TATAATGTAG TCATGAGGAG GGTGTTCAAA
    ATTCTGGACC TGAAACTTGT TGGGAGAAAT TTTTATGACC CAGCAAGTTC AACAGTGCTG
    CAGCAGTACA GACTGCAGGT GTGGCCAGGC TATGCTGCAA ATATACGCAA AACTGACGGT
    GGGCTATTTC TTTTGGTTGA CATCACCCAC AAGATTATCC GCAGTGATTC TGTGCTGGAC
    ATCATGAACA TTCTATATCA GCAGAGCCCT GAGAACTTTC AGGATGAGGT TACCAAGCAG
    CTGGTTGGCA GCATTGTGAT CACCAGATAC AACAACCGTA CCTACCGAAT AGATGACATT
    GAATGGAACA TGAGCCCCAA GGACTCCTTC TCTATGTCAG ATGGAAGCAA AGTCAGTTTC
    ATTGATTATT ACAGCAAGAA CTATGGCATA ACTGTTAAGG AGCTGGACCA ACCCTTGCTG
    CTTCACAGAC CTAGTGAAAG GAAGGCACCA AAGGGCAAGG CACTTGATAT TGTCCTCCTC
    TTACCAGAAC TTGCTTTTAT GACTGGCATC CCTGAAAAAA TGAGAAAAGA CTTTCGGGCC
    ATGAAGGATC TGACACAGCA GATTCATTTA AGCCCAAAAC AGCATCATAT CTCTTTGGGG
    AAATTGCTAA AACGTATTGA AAGCAGTGCG GATGCAAAGA ATGAACTGCA GCGTTGGGGC
    TTATTCCTAG ACACTGATAT TCACATGACC ACTGGTAGGA TTTTACCAAT AGAGAAAATA
    AATCTGCGTA ATAACTCATT TCCTGCAGGA GAAGATCTCA ATTGGAACAG GGAAGTGACA
    CGTGAGGCAT GCCGTTCCTC AGTGCATCTT CTTTATTGGG CAATGATATA TCCTAAACGG
    GCTTCAGCAC AGGCACAAGA ATTGTCTAGT ATGCTGGAGA GGATTGGAGG ACCTATTGGA
    ATACGAGTAA ACCATCCTAA CTGTGTGGAG CTCCGGGATG ACCGTGTCGA AACGTACGCG
    CGCAGCATCA AATCACTGTT AGAGGGTGAG GGCAAAGTTC AGCTACTAGT GTGTCTTATA
    TCTGGGACCA GAGATGACCT GTACGGTGCT ATTAAGAAAT TGTGTTGTGT ACAGAACCCA
    GTTCCATCAC AGGTGATTAA TACCAGAACC ATCTCCCAGC CCCAGAAGCT CAGGAGCATT
    GCACAGAAAA TTCTGCTTCA GATTAACTGC AAGCTGGGAG GGGAACTGTG GGGAGTAGAC
    ATCCCTCTGA AAAGTGTGAT GGTGATTGGG ATGGATGTTT ATCACGACCC CAGCAGAGGC
    ATGCGCTCTG TATTAGGCTT TGTTGCTAGC ATCAACAGCT GCCTGACTGC TTGGTATTCT
    CGTGTGGTCT TTCAGCTTCC TAACCAGGAG ATCATGGATA GCCTAAAGCT TTGCCTTGTG
    GCAGCCCTGC AGAAATTCTT TGAGGTGAAT CACAGTCTTC CAGAGAAGAT TGTGGTGTAT
    CGAGATGGCG TATCTGATGG GCAACTTAAC ACGGTGGAAA ACTATGAGAT CCCACAGCTT
    CAAACTTGCT TCCAGACATT TGATAACTAT AATCCACGTA TGGTGGTTAT TGTAGTACAG
    AAAAGGGTTA GCACTAATCT GTACTCTACC GCCACTGGGC AGTTTCTTAC CCCTCAACCT
    GGGACTGTTA TAGACCATAC AGTCACCAAC CGCAAGTGGA TAGATTTCTT CCTGATGTCT
    CATCATGTGA GACAAGGCTG CGGGATCCCC ACCCACTACA TTTGTGTCAT GAATACAGCA
    AATTTGGGTC CTGACCATTT ACAGAGGTTG ACCTTCAAGC TTTGCCACAT GTACTGGAAC
    TGGCCTGGGA CCATCCGAGT GCCAGCTCCA TGTAAATACG CACACAAGCT GGCCTTCCTT
    TCCGGTCAGT TTCTGCACCA CGAGCCTTCC ATAAAGCTGT GTGACAAGCT TTTCTTCCTT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa43020
    Clone ID Related Accession (Same CDS sequence) XM_018091922.1
    Accession Version XM_018091922.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2766bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product piwi-like protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGGAAGTC AGCAGCGGGT TACCGCCCAA AAAATGGATC CTACCAGACC TCCATTCAGG 
    GGTTCCCCCT TTCATACACC ATTGGGAGTG CGTCCTCCAG TTCTAGAAAC TAAAGAAGAA
    GGGCCACATG GAAGAGCAGT CCTATTACCA CGGGGCCGTG CATTATTGGG TGCATCAGCA
    CCTTCTTCTG ATACTACCCA AAGAGATCCA TCTGACTCTA GGAATGTTCT CCCTGCTCTA
    TTTCGTGGTA TGGGAATTGA AACAAAGCCC AGTGGGATTC CTGGCCGTGG TGGGCCAGTC
    TTTGGCAGGG GGTTTCTTTC TACTATGTCT TCAGGAGATG GCCCTGATCA GCCACTTTCA
    GAACCCTCTA TAAGACCCTC CCTCTCAACC CGTGTTCAGC AAGCAAGTGA TTTTAGCACA
    GAGAGGGTTG CACTTGGGAG AGCCAGGTTT CCAATACCTC CGGTGAGTAT GGAAAAGCCA
    CATGTACCCA CTGGAAGGGG TTTGTTATTT CCCTCTGTTC TACCCACTTT GACATCTGAC
    CCAGTACCTA AAGAAAGCCC TATCGCTACA CTTCAAATTG AGAAGGAAGA GAAATGGGAG
    CCTTTACCAA AGAAAGGCAG CAAAGGTAGC CCCTGTCAGC TTGGACTGAA CCTCATTAAA
    ATTAACTTCC AGAATGAAGC CGTATATCAG TACCATGTCA CATTTACTCC CATAGTGGAG
    TGCCGTAGTA TGCGTTTTGG TATGATGAAG GACCACCGCT CTGTGACAGG ACCAGTAACT
    GCATTCGACG GTTCTATACT TTACCTTCCT GTCAAACTTG CACAGACAGT TGAGCTGGAG
    AGTGAACGCC GGACAGATGG ACAAAAAATA AAGATAACCA TACAGATGAC AAAGATTCTG
    GATCCCAGTT CAGACTTGTG CCTTCCTTTT TATAATGTAG TCATGAGGAG GGTGTTCAAA
    ATTCTGGACC TGAAACTTGT TGGGAGAAAT TTTTATGACC CAGCAAGTTC AACAGTGCTG
    CAGCAGTACA GACTGCAGGT GTGGCCAGGC TATGCTGCAA ATATACGCAA AACTGACGGT
    GGGCTATTTC TTTTGGTTGA CATCACCCAC AAGATTATCC GCAGTGATTC TGTGCTGGAC
    ATCATGAACA TTCTATATCA GCAGAGCCCT GAGAACTTTC AGGATGAGGT TACCAAGCAG
    CTGGTTGGCA GCATTGTGAT CACCAGATAC AACAACCGTA CCTACCGAAT AGATGACATT
    GAATGGAACA TGAGCCCCAA GGACTCCTTC TCTATGTCAG ATGGAAGCAA AGTCAGTTTC
    ATTGATTATT ACAGCAAGAA CTATGGCATA ACTGTTAAGG AGCTGGACCA ACCCTTGCTG
    CTTCACAGAC CTAGTGAAAG GAAGGCACCA AAGGGCAAGG CACTTGATAT TGTCCTCCTC
    TTACCAGAAC TTGCTTTTAT GACTGGCATC CCTGAAAAAA TGAGAAAAGA CTTTCGGGCC
    ATGAAGGATC TGACACAGCA GATTCATTTA AGCCCAAAAC AGCATCATAT CTCTTTGGGG
    AAATTGCTAA AACGTATTGA AAGCAGTGCG GATGCAAAGA ATGAACTGCA GCGTTGGGGC
    TTATTCCTAG ACACTGATAT TCACATGACC ACTGGTAGGA TTTTACCAAT AGAGAAAATA
    AATCTGCGTA ATAACTCATT TCCTGCAGGA GAAGATCTCA ATTGGAACAG GGAAGTGACA
    CGTGAGGCAT GCCGTTCCTC AGTGCATCTT CTTTATTGGG CAATGATATA TCCTAAACGG
    GCTTCAGCAC AGGCACAAGA ATTGTCTAGT ATGCTGGAGA GGATTGGAGG ACCTATTGGA
    ATACGAGTAA ACCATCCTAA CTGTGTGGAG CTCCGGGATG ACCGTGTCGA AACGTACGCG
    CGCAGCATCA AATCACTGTT AGAGGGTGAG GGCAAAGTTC AGCTACTAGT GTGTCTTATA
    TCTGGGACCA GAGATGACCT GTACGGTGCT ATTAAGAAAT TGTGTTGTGT ACAGAACCCA
    GTTCCATCAC AGAAAAGTGT GATGGTGATT GGGATGGATG TTTATCACGA CCCCAGCAGA
    GGCATGCGCT CTGTATTAGG CTTTGTTGCT AGCATCAACA GCTGCCTGAC TGCTTGGTAT
    TCTCGTGTGG TCTTTCAGCT TCCTAACCAG GAGATCATGG ATAGCCTAAA GCTTTGCCTT
    GTGGCAGCCC TGCAGAAATT CTTTGAGGTG AATCACAGTC TTCCAGAGAA GATTGTGGTG
    TATCGAGATG GCGTATCTGA TGGGCAACTT AACACGGTGG AAAACTATGA GATCCCACAG
    CTTCAAACTT GCTTCCAGAC ATTTGATAAC TATAATCCAC GTATGGTGGT TATTGTAGTA
    CAGAAAAGGG TTAGCACTAA TCTGTACTCT ACCGCCACTG GGCAGTTTCT TACCCCTCAA
    CCTGGGACTG TTATAGACCA TACAGTCACC AACCGCAAGT GGATAGATTT CTTCCTGATG
    TCTCATCATG TGAGACAAGG CTGCGGGATC CCCACCCACT ACATTTGTGT CATGAATACA
    GCAAATTTGG GTCCTGACCA TTTACAGAGG TTGACCTTCA AGCTTTGCCA CATGTACTGG
    AACTGGCCTG GGACCATCCG AGTGCCAGCT CCATGTAAAT ACGCACACAA GCTGGCCTTC
    CTTTCCGGTC AGTTTCTGCA CCACGAGCCT TCCATAAAGC TGTGTGACAA GCTTTTCTTC
    CTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947411.1
    CDS71..2836
    Translation

    Target ORF information:

    RefSeq Version XM_018091922.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis piwi-like RNA-mediated gene silencing 2 (piwil2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091922.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGGAAGTC AGCAGCGGGT TACCGCCCAA AAAATGGATC CTACCAGACC TCCATTCAGG 
    GGTTCCCCCT TTCATACACC ATTGGGAGTG CGTCCTCCAG TTCTAGAAAC TAAAGAAGAA
    GGGCCACATG GAAGAGCAGT CCTATTACCA CGGGGCCGTG CATTATTGGG TGCATCAGCA
    CCTTCTTCTG ATACTACCCA AAGAGATCCA TCTGACTCTA GGAATGTTCT CCCTGCTCTA
    TTTCGTGGTA TGGGAATTGA AACAAAGCCC AGTGGGATTC CTGGCCGTGG TGGGCCAGTC
    TTTGGCAGGG GGTTTCTTTC TACTATGTCT TCAGGAGATG GCCCTGATCA GCCACTTTCA
    GAACCCTCTA TAAGACCCTC CCTCTCAACC CGTGTTCAGC AAGCAAGTGA TTTTAGCACA
    GAGAGGGTTG CACTTGGGAG AGCCAGGTTT CCAATACCTC CGGTGAGTAT GGAAAAGCCA
    CATGTACCCA CTGGAAGGGG TTTGTTATTT CCCTCTGTTC TACCCACTTT GACATCTGAC
    CCAGTACCTA AAGAAAGCCC TATCGCTACA CTTCAAATTG AGAAGGAAGA GAAATGGGAG
    CCTTTACCAA AGAAAGGCAG CAAAGGTAGC CCCTGTCAGC TTGGACTGAA CCTCATTAAA
    ATTAACTTCC AGAATGAAGC CGTATATCAG TACCATGTCA CATTTACTCC CATAGTGGAG
    TGCCGTAGTA TGCGTTTTGG TATGATGAAG GACCACCGCT CTGTGACAGG ACCAGTAACT
    GCATTCGACG GTTCTATACT TTACCTTCCT GTCAAACTTG CACAGACAGT TGAGCTGGAG
    AGTGAACGCC GGACAGATGG ACAAAAAATA AAGATAACCA TACAGATGAC AAAGATTCTG
    GATCCCAGTT CAGACTTGTG CCTTCCTTTT TATAATGTAG TCATGAGGAG GGTGTTCAAA
    ATTCTGGACC TGAAACTTGT TGGGAGAAAT TTTTATGACC CAGCAAGTTC AACAGTGCTG
    CAGCAGTACA GACTGCAGGT GTGGCCAGGC TATGCTGCAA ATATACGCAA AACTGACGGT
    GGGCTATTTC TTTTGGTTGA CATCACCCAC AAGATTATCC GCAGTGATTC TGTGCTGGAC
    ATCATGAACA TTCTATATCA GCAGAGCCCT GAGAACTTTC AGGATGAGGT TACCAAGCAG
    CTGGTTGGCA GCATTGTGAT CACCAGATAC AACAACCGTA CCTACCGAAT AGATGACATT
    GAATGGAACA TGAGCCCCAA GGACTCCTTC TCTATGTCAG ATGGAAGCAA AGTCAGTTTC
    ATTGATTATT ACAGCAAGAA CTATGGCATA ACTGTTAAGG AGCTGGACCA ACCCTTGCTG
    CTTCACAGAC CTAGTGAAAG GAAGGCACCA AAGGGCAAGG CACTTGATAT TGTCCTCCTC
    TTACCAGAAC TTGCTTTTAT GACTGGCATC CCTGAAAAAA TGAGAAAAGA CTTTCGGGCC
    ATGAAGGATC TGACACAGCA GATTCATTTA AGCCCAAAAC AGCATCATAT CTCTTTGGGG
    AAATTGCTAA AACGTATTGA AAGCAGTGCG GATGCAAAGA ATGAACTGCA GCGTTGGGGC
    TTATTCCTAG ACACTGATAT TCACATGACC ACTGGTAGGA TTTTACCAAT AGAGAAAATA
    AATCTGCGTA ATAACTCATT TCCTGCAGGA GAAGATCTCA ATTGGAACAG GGAAGTGACA
    CGTGAGGCAT GCCGTTCCTC AGTGCATCTT CTTTATTGGG CAATGATATA TCCTAAACGG
    GCTTCAGCAC AGGCACAAGA ATTGTCTAGT ATGCTGGAGA GGATTGGAGG ACCTATTGGA
    ATACGAGTAA ACCATCCTAA CTGTGTGGAG CTCCGGGATG ACCGTGTCGA AACGTACGCG
    CGCAGCATCA AATCACTGTT AGAGGGTGAG GGCAAAGTTC AGCTACTAGT GTGTCTTATA
    TCTGGGACCA GAGATGACCT GTACGGTGCT ATTAAGAAAT TGTGTTGTGT ACAGAACCCA
    GTTCCATCAC AGAAAAGTGT GATGGTGATT GGGATGGATG TTTATCACGA CCCCAGCAGA
    GGCATGCGCT CTGTATTAGG CTTTGTTGCT AGCATCAACA GCTGCCTGAC TGCTTGGTAT
    TCTCGTGTGG TCTTTCAGCT TCCTAACCAG GAGATCATGG ATAGCCTAAA GCTTTGCCTT
    GTGGCAGCCC TGCAGAAATT CTTTGAGGTG AATCACAGTC TTCCAGAGAA GATTGTGGTG
    TATCGAGATG GCGTATCTGA TGGGCAACTT AACACGGTGG AAAACTATGA GATCCCACAG
    CTTCAAACTT GCTTCCAGAC ATTTGATAAC TATAATCCAC GTATGGTGGT TATTGTAGTA
    CAGAAAAGGG TTAGCACTAA TCTGTACTCT ACCGCCACTG GGCAGTTTCT TACCCCTCAA
    CCTGGGACTG TTATAGACCA TACAGTCACC AACCGCAAGT GGATAGATTT CTTCCTGATG
    TCTCATCATG TGAGACAAGG CTGCGGGATC CCCACCCACT ACATTTGTGT CATGAATACA
    GCAAATTTGG GTCCTGACCA TTTACAGAGG TTGACCTTCA AGCTTTGCCA CATGTACTGG
    AACTGGCCTG GGACCATCCG AGTGCCAGCT CCATGTAAAT ACGCACACAA GCTGGCCTTC
    CTTTCCGGTC AGTTTCTGCA CCACGAGCCT TCCATAAAGC TGTGTGACAA GCTTTTCTTC
    CTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.