dgka cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following dgka gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dgka cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa06017 XM_018091694.1
Latest version!
Xenopus tropicalis diacylglycerol kinase alpha (dgka), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OXa06017 XM_018091693.1
Latest version!
Xenopus tropicalis diacylglycerol kinase alpha (dgka), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OXa06017 XM_018091692.1
Latest version!
Xenopus tropicalis diacylglycerol kinase alpha (dgka), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OXa06017 XM_018091697.1
Latest version!
Xenopus tropicalis diacylglycerol kinase alpha (dgka), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OXa06017 XM_012957814.2
Latest version!
Xenopus tropicalis diacylglycerol kinase alpha (dgka), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OXa06017 XM_018091698.1
Latest version!
Xenopus tropicalis diacylglycerol kinase alpha (dgka), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OXa06017 XM_012957813.2
Latest version!
Xenopus tropicalis diacylglycerol kinase alpha (dgka), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OXa06017 XM_012957812.2
Latest version!
Xenopus tropicalis diacylglycerol kinase alpha (dgka), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OXa06017 XM_012957811.2
Latest version!
Xenopus tropicalis diacylglycerol kinase alpha (dgka), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OXa06017 XM_012957810.2
Latest version!
Xenopus tropicalis diacylglycerol kinase alpha (dgka), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OXa06017 XM_012957809.2
Latest version!
Xenopus tropicalis diacylglycerol kinase alpha (dgka), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OXa06017 XM_012957808.2
Latest version!
Xenopus tropicalis diacylglycerol kinase alpha (dgka), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OXa42842 XM_018091695.1
Latest version!
Xenopus tropicalis diacylglycerol kinase alpha (dgka), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
OXa42843 XM_018091696.1
Latest version!
Xenopus tropicalis diacylglycerol kinase alpha (dgka), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
OXa52684 XM_012957810.3
Latest version!
Xenopus tropicalis diacylglycerol kinase alpha (dgka), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa52684 XM_012957811.3
Latest version!
Xenopus tropicalis diacylglycerol kinase alpha (dgka), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa52684 XM_012957809.3
Latest version!
Xenopus tropicalis diacylglycerol kinase alpha (dgka), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa52684 XM_012957812.3
Latest version!
Xenopus tropicalis diacylglycerol kinase alpha (dgka), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa52684 XM_012957813.3
Latest version!
Xenopus tropicalis diacylglycerol kinase alpha (dgka), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa52684 XM_012957814.3
Latest version!
Xenopus tropicalis diacylglycerol kinase alpha (dgka), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa52684 XM_012957808.3
Latest version!
Xenopus tropicalis diacylglycerol kinase alpha (dgka), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa52684 XM_031897165.1
Latest version!
Xenopus tropicalis diacylglycerol kinase alpha (dgka), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa52684 XM_018091693.2
Latest version!
Xenopus tropicalis diacylglycerol kinase alpha (dgka), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa06017
    Clone ID Related Accession (Same CDS sequence) XM_012957810.2 , XM_012957812.2 , XM_012957808.2 , XM_018091693.1 , XM_012957809.2 , XM_018091697.1 , XM_012957811.2 , XM_018091692.1 , XM_012957813.2 , XM_018091694.1 , XM_012957814.2 , XM_018091698.1
    Accession Version XM_012957810.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2235bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product diacylglycerol kinase alpha isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957810.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCGACG AGAACAAGGA GTGGGCAATC CTGAGCCCGG AGGACTTCAC CCAACTGCAG 
    AAGTACATTG ATTACAGCAC CAAGAAGGTT AAAGATGTTC AGCAAGAGTT CTATGGGGAT
    GGTACATCGT GCCCTCACTT GGTTGAAGAC ACAATAGATT TTGAAGCATT CAAATCCTTT
    TTGAAAACAT ACCTGGAAGT AGATGACATT CCCAACAATC TCTGTGAGCG TCTCTTCATG
    TCTTTTCAAG CAACCCCAAT TGAGAGCAAA AAAAGTGGGG AGGATGAGAC AGAAGGCATA
    AAAGGTAGTG GCCGTATCAG TCTAAATGAT GTGTCCTGTT ATTTCTCTTT GCTGGAAAGT
    GGCACTGCTG AGGATAAACT GGCATTCACC TTTAAATTGT ATGACAAAGA CAGCAATGGT
    CTTCTGGACA GCTCTGAGGT GGACAGGATT ATAACACAAA TGATGCGTGT AGCGGAATAT
    CTGGACTGGG ACATCACGGA ACTCAAACCT ATGTTAGAGG AGATGATGAG ACAAGTGGAC
    TATGATGGAA ATGGAATAGT CTCTTATGAA GAATGGGTTA AAGGAGGACT CACAACAGTG
    CCTCTGCTGG TGCTTCTAGG AATGGAAGTT AATATAAAAG ACGATGGGCA GCACCTGTGG
    CGCCTGAAGC ATTTCAACCG CCCTGTGTAT TGCAATGTGT GTGAAGCTCT TCTTTTTGGG
    GTCAGGAAGC AGGGCCTGTG TTGCACTTTC TGTAAGTTTA CCGTTCATGA ACGCTGTGCC
    CGCAAAGCGC CTAATTCCTG CATAAGCACG TATGCCAAAT CACGCAAGGA TTTCAGCGAG
    CAGGCCCATG TTTGGATGAG AGGTGGATGT GAGGCCAGCA AGTGTGACAA ATGCCAAAAA
    AAAATAAAAA GTTTCCAGAG CCTGACTGGG CAGCGCTGTG TGTGGTGTCA TACAAAGGTT
    CATGATGAAT GTGCCTCATC CCCACTGTGT GATGGTGGAA TCTTAAAGGA CCACATATTA
    CCTCCTGCTT GCATATATCC AGTAATCCTG GAACGACAGA ATAGCCAAAA AAATCTTCTA
    GCTTCCTCAG AAGAAGCATC ACAGACGTTT AGTACCCCAG AAGGACAAGT CCTGCGGATT
    GCCCCATTAC CAGGTACACA CCCTCTACTT GTCTTTGTAA ACCCAAAGAG TGGAGGGAAG
    CAGGGGGAAA GAGTGCTGAG GAAATTCCAG TATCTGCTAA ATCCCAGACA AGTCCATAAC
    TTGATAAAGG GAGGCCCTTC CCCAGGGCTG AATTTTTTCC GGGATGTCCC AGAATATCGT
    ATCTTAGTAT GCGGCGGTGA TGGAACAGTC GGTTGGATCC TGGATGCTAT TGACAAGGCA
    AACCTTCCAT ATAGACCACC TGTAGCTGTG CTGCCCCTCG GTACAGGAAA TGACCTTGCG
    CGATGTCTCA GCTGGGGAGG TGGTTATGAT GGAGAAAATC TAATGAAGTT CTTGAAGGAC
    ATTGAAAAGA GTGTACCCAT TAAAATGGAC AGATGGAACA TTGAGGTTAT ACCTGAAAAC
    CCAGATGAGA AGGGAGACCC AGTTCCTTAC GAGATTATAA ACAACTACTT TTCCATTGGG
    GTGGATGCAT CTATAGCTCA CAGATTCCAT AATATGAGGG AAAAATATCC AGAGAAATTC
    AATAGCAGAA TGAAGAACAA ATTGTGGTAT TTTGAGTTTG CTACATCAGA GACTGTTTTC
    TCTACCTGTA AAAAACTTAA AGAGGTCATT ACAATTGAGT GTTGTGGCTC CCCGTTGAAA
    CTCAGCTCTC TGTCCCTGGA AGGCATCGCA GTTATAAATA TTCCAAGCAT GCATGGGGGT
    TCCAACCTAT GGGGAGAGAC TAAGAAACAC TGGGGAGAAC CTCGCAATGC TTCATGTCAT
    AATGCCCCTG AAGCCATTAC TGACCCTGAT GCCTTAAAGT CATGTGTACA AGACCTCAGT
    GACAAGAGGC TGGAGGTAGT AGGACTTGAG GGTGCTATTG AGATGGGGCA GATTTATACT
    GGACTGAAGA GTGCTGGCAA ACGACTTGCA ACATGTTCTG AACTAACAAT AAGGACCACA
    AAGCCCCTCC CAATGCAAAT TGATGGCGAG CCCTGGATGC AGCCACCCTG CACGATAAAA
    ATCACACATA AGAACCAGGC ACCGATGCTG CGTGCCCCTG CGCCCCGTTC TTCAAGCTTT
    TTCTCCCTGA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813264.1
    CDS489..2723
    Translation

    Target ORF information:

    RefSeq Version XM_012957810.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis diacylglycerol kinase alpha (dgka), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957810.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCGACG AGAACAAGGA GTGGGCAATC CTGAGCCCGG AGGACTTCAC CCAACTGCAG 
    AAGTACATTG ATTACAGCAC CAAGAAGGTT AAAGATGTTC AGCAAGAGTT CTATGGGGAT
    GGTACATCGT GCCCTCACTT GGTTGAAGAC ACAATAGATT TTGAAGCATT CAAATCCTTT
    TTGAAAACAT ACCTGGAAGT AGATGACATT CCCAACAATC TCTGTGAGCG TCTCTTCATG
    TCTTTTCAAG CAACCCCAAT TGAGAGCAAA AAAAGTGGGG AGGATGAGAC AGAAGGCATA
    AAAGGTAGTG GCCGTATCAG TCTAAATGAT GTGTCCTGTT ATTTCTCTTT GCTGGAAAGT
    GGCACTGCTG AGGATAAACT GGCATTCACC TTTAAATTGT ATGACAAAGA CAGCAATGGT
    CTTCTGGACA GCTCTGAGGT GGACAGGATT ATAACACAAA TGATGCGTGT AGCGGAATAT
    CTGGACTGGG ACATCACGGA ACTCAAACCT ATGTTAGAGG AGATGATGAG ACAAGTGGAC
    TATGATGGAA ATGGAATAGT CTCTTATGAA GAATGGGTTA AAGGAGGACT CACAACAGTG
    CCTCTGCTGG TGCTTCTAGG AATGGAAGTT AATATAAAAG ACGATGGGCA GCACCTGTGG
    CGCCTGAAGC ATTTCAACCG CCCTGTGTAT TGCAATGTGT GTGAAGCTCT TCTTTTTGGG
    GTCAGGAAGC AGGGCCTGTG TTGCACTTTC TGTAAGTTTA CCGTTCATGA ACGCTGTGCC
    CGCAAAGCGC CTAATTCCTG CATAAGCACG TATGCCAAAT CACGCAAGGA TTTCAGCGAG
    CAGGCCCATG TTTGGATGAG AGGTGGATGT GAGGCCAGCA AGTGTGACAA ATGCCAAAAA
    AAAATAAAAA GTTTCCAGAG CCTGACTGGG CAGCGCTGTG TGTGGTGTCA TACAAAGGTT
    CATGATGAAT GTGCCTCATC CCCACTGTGT GATGGTGGAA TCTTAAAGGA CCACATATTA
    CCTCCTGCTT GCATATATCC AGTAATCCTG GAACGACAGA ATAGCCAAAA AAATCTTCTA
    GCTTCCTCAG AAGAAGCATC ACAGACGTTT AGTACCCCAG AAGGACAAGT CCTGCGGATT
    GCCCCATTAC CAGGTACACA CCCTCTACTT GTCTTTGTAA ACCCAAAGAG TGGAGGGAAG
    CAGGGGGAAA GAGTGCTGAG GAAATTCCAG TATCTGCTAA ATCCCAGACA AGTCCATAAC
    TTGATAAAGG GAGGCCCTTC CCCAGGGCTG AATTTTTTCC GGGATGTCCC AGAATATCGT
    ATCTTAGTAT GCGGCGGTGA TGGAACAGTC GGTTGGATCC TGGATGCTAT TGACAAGGCA
    AACCTTCCAT ATAGACCACC TGTAGCTGTG CTGCCCCTCG GTACAGGAAA TGACCTTGCG
    CGATGTCTCA GCTGGGGAGG TGGTTATGAT GGAGAAAATC TAATGAAGTT CTTGAAGGAC
    ATTGAAAAGA GTGTACCCAT TAAAATGGAC AGATGGAACA TTGAGGTTAT ACCTGAAAAC
    CCAGATGAGA AGGGAGACCC AGTTCCTTAC GAGATTATAA ACAACTACTT TTCCATTGGG
    GTGGATGCAT CTATAGCTCA CAGATTCCAT AATATGAGGG AAAAATATCC AGAGAAATTC
    AATAGCAGAA TGAAGAACAA ATTGTGGTAT TTTGAGTTTG CTACATCAGA GACTGTTTTC
    TCTACCTGTA AAAAACTTAA AGAGGTCATT ACAATTGAGT GTTGTGGCTC CCCGTTGAAA
    CTCAGCTCTC TGTCCCTGGA AGGCATCGCA GTTATAAATA TTCCAAGCAT GCATGGGGGT
    TCCAACCTAT GGGGAGAGAC TAAGAAACAC TGGGGAGAAC CTCGCAATGC TTCATGTCAT
    AATGCCCCTG AAGCCATTAC TGACCCTGAT GCCTTAAAGT CATGTGTACA AGACCTCAGT
    GACAAGAGGC TGGAGGTAGT AGGACTTGAG GGTGCTATTG AGATGGGGCA GATTTATACT
    GGACTGAAGA GTGCTGGCAA ACGACTTGCA ACATGTTCTG AACTAACAAT AAGGACCACA
    AAGCCCCTCC CAATGCAAAT TGATGGCGAG CCCTGGATGC AGCCACCCTG CACGATAAAA
    ATCACACATA AGAACCAGGC ACCGATGCTG CGTGCCCCTG CGCCCCGTTC TTCAAGCTTT
    TTCTCCCTGA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa06017
    Clone ID Related Accession (Same CDS sequence) XM_012957810.2 , XM_012957812.2 , XM_012957808.2 , XM_018091693.1 , XM_012957809.2 , XM_018091697.1 , XM_012957811.2 , XM_018091692.1 , XM_012957813.2 , XM_018091694.1 , XM_012957814.2 , XM_018091698.1
    Accession Version XM_012957812.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2235bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product diacylglycerol kinase alpha isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957812.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCGACG AGAACAAGGA GTGGGCAATC CTGAGCCCGG AGGACTTCAC CCAACTGCAG 
    AAGTACATTG ATTACAGCAC CAAGAAGGTT AAAGATGTTC AGCAAGAGTT CTATGGGGAT
    GGTACATCGT GCCCTCACTT GGTTGAAGAC ACAATAGATT TTGAAGCATT CAAATCCTTT
    TTGAAAACAT ACCTGGAAGT AGATGACATT CCCAACAATC TCTGTGAGCG TCTCTTCATG
    TCTTTTCAAG CAACCCCAAT TGAGAGCAAA AAAAGTGGGG AGGATGAGAC AGAAGGCATA
    AAAGGTAGTG GCCGTATCAG TCTAAATGAT GTGTCCTGTT ATTTCTCTTT GCTGGAAAGT
    GGCACTGCTG AGGATAAACT GGCATTCACC TTTAAATTGT ATGACAAAGA CAGCAATGGT
    CTTCTGGACA GCTCTGAGGT GGACAGGATT ATAACACAAA TGATGCGTGT AGCGGAATAT
    CTGGACTGGG ACATCACGGA ACTCAAACCT ATGTTAGAGG AGATGATGAG ACAAGTGGAC
    TATGATGGAA ATGGAATAGT CTCTTATGAA GAATGGGTTA AAGGAGGACT CACAACAGTG
    CCTCTGCTGG TGCTTCTAGG AATGGAAGTT AATATAAAAG ACGATGGGCA GCACCTGTGG
    CGCCTGAAGC ATTTCAACCG CCCTGTGTAT TGCAATGTGT GTGAAGCTCT TCTTTTTGGG
    GTCAGGAAGC AGGGCCTGTG TTGCACTTTC TGTAAGTTTA CCGTTCATGA ACGCTGTGCC
    CGCAAAGCGC CTAATTCCTG CATAAGCACG TATGCCAAAT CACGCAAGGA TTTCAGCGAG
    CAGGCCCATG TTTGGATGAG AGGTGGATGT GAGGCCAGCA AGTGTGACAA ATGCCAAAAA
    AAAATAAAAA GTTTCCAGAG CCTGACTGGG CAGCGCTGTG TGTGGTGTCA TACAAAGGTT
    CATGATGAAT GTGCCTCATC CCCACTGTGT GATGGTGGAA TCTTAAAGGA CCACATATTA
    CCTCCTGCTT GCATATATCC AGTAATCCTG GAACGACAGA ATAGCCAAAA AAATCTTCTA
    GCTTCCTCAG AAGAAGCATC ACAGACGTTT AGTACCCCAG AAGGACAAGT CCTGCGGATT
    GCCCCATTAC CAGGTACACA CCCTCTACTT GTCTTTGTAA ACCCAAAGAG TGGAGGGAAG
    CAGGGGGAAA GAGTGCTGAG GAAATTCCAG TATCTGCTAA ATCCCAGACA AGTCCATAAC
    TTGATAAAGG GAGGCCCTTC CCCAGGGCTG AATTTTTTCC GGGATGTCCC AGAATATCGT
    ATCTTAGTAT GCGGCGGTGA TGGAACAGTC GGTTGGATCC TGGATGCTAT TGACAAGGCA
    AACCTTCCAT ATAGACCACC TGTAGCTGTG CTGCCCCTCG GTACAGGAAA TGACCTTGCG
    CGATGTCTCA GCTGGGGAGG TGGTTATGAT GGAGAAAATC TAATGAAGTT CTTGAAGGAC
    ATTGAAAAGA GTGTACCCAT TAAAATGGAC AGATGGAACA TTGAGGTTAT ACCTGAAAAC
    CCAGATGAGA AGGGAGACCC AGTTCCTTAC GAGATTATAA ACAACTACTT TTCCATTGGG
    GTGGATGCAT CTATAGCTCA CAGATTCCAT AATATGAGGG AAAAATATCC AGAGAAATTC
    AATAGCAGAA TGAAGAACAA ATTGTGGTAT TTTGAGTTTG CTACATCAGA GACTGTTTTC
    TCTACCTGTA AAAAACTTAA AGAGGTCATT ACAATTGAGT GTTGTGGCTC CCCGTTGAAA
    CTCAGCTCTC TGTCCCTGGA AGGCATCGCA GTTATAAATA TTCCAAGCAT GCATGGGGGT
    TCCAACCTAT GGGGAGAGAC TAAGAAACAC TGGGGAGAAC CTCGCAATGC TTCATGTCAT
    AATGCCCCTG AAGCCATTAC TGACCCTGAT GCCTTAAAGT CATGTGTACA AGACCTCAGT
    GACAAGAGGC TGGAGGTAGT AGGACTTGAG GGTGCTATTG AGATGGGGCA GATTTATACT
    GGACTGAAGA GTGCTGGCAA ACGACTTGCA ACATGTTCTG AACTAACAAT AAGGACCACA
    AAGCCCCTCC CAATGCAAAT TGATGGCGAG CCCTGGATGC AGCCACCCTG CACGATAAAA
    ATCACACATA AGAACCAGGC ACCGATGCTG CGTGCCCCTG CGCCCCGTTC TTCAAGCTTT
    TTCTCCCTGA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813266.1
    CDS664..2898
    Translation

    Target ORF information:

    RefSeq Version XM_012957812.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis diacylglycerol kinase alpha (dgka), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957812.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCGACG AGAACAAGGA GTGGGCAATC CTGAGCCCGG AGGACTTCAC CCAACTGCAG 
    AAGTACATTG ATTACAGCAC CAAGAAGGTT AAAGATGTTC AGCAAGAGTT CTATGGGGAT
    GGTACATCGT GCCCTCACTT GGTTGAAGAC ACAATAGATT TTGAAGCATT CAAATCCTTT
    TTGAAAACAT ACCTGGAAGT AGATGACATT CCCAACAATC TCTGTGAGCG TCTCTTCATG
    TCTTTTCAAG CAACCCCAAT TGAGAGCAAA AAAAGTGGGG AGGATGAGAC AGAAGGCATA
    AAAGGTAGTG GCCGTATCAG TCTAAATGAT GTGTCCTGTT ATTTCTCTTT GCTGGAAAGT
    GGCACTGCTG AGGATAAACT GGCATTCACC TTTAAATTGT ATGACAAAGA CAGCAATGGT
    CTTCTGGACA GCTCTGAGGT GGACAGGATT ATAACACAAA TGATGCGTGT AGCGGAATAT
    CTGGACTGGG ACATCACGGA ACTCAAACCT ATGTTAGAGG AGATGATGAG ACAAGTGGAC
    TATGATGGAA ATGGAATAGT CTCTTATGAA GAATGGGTTA AAGGAGGACT CACAACAGTG
    CCTCTGCTGG TGCTTCTAGG AATGGAAGTT AATATAAAAG ACGATGGGCA GCACCTGTGG
    CGCCTGAAGC ATTTCAACCG CCCTGTGTAT TGCAATGTGT GTGAAGCTCT TCTTTTTGGG
    GTCAGGAAGC AGGGCCTGTG TTGCACTTTC TGTAAGTTTA CCGTTCATGA ACGCTGTGCC
    CGCAAAGCGC CTAATTCCTG CATAAGCACG TATGCCAAAT CACGCAAGGA TTTCAGCGAG
    CAGGCCCATG TTTGGATGAG AGGTGGATGT GAGGCCAGCA AGTGTGACAA ATGCCAAAAA
    AAAATAAAAA GTTTCCAGAG CCTGACTGGG CAGCGCTGTG TGTGGTGTCA TACAAAGGTT
    CATGATGAAT GTGCCTCATC CCCACTGTGT GATGGTGGAA TCTTAAAGGA CCACATATTA
    CCTCCTGCTT GCATATATCC AGTAATCCTG GAACGACAGA ATAGCCAAAA AAATCTTCTA
    GCTTCCTCAG AAGAAGCATC ACAGACGTTT AGTACCCCAG AAGGACAAGT CCTGCGGATT
    GCCCCATTAC CAGGTACACA CCCTCTACTT GTCTTTGTAA ACCCAAAGAG TGGAGGGAAG
    CAGGGGGAAA GAGTGCTGAG GAAATTCCAG TATCTGCTAA ATCCCAGACA AGTCCATAAC
    TTGATAAAGG GAGGCCCTTC CCCAGGGCTG AATTTTTTCC GGGATGTCCC AGAATATCGT
    ATCTTAGTAT GCGGCGGTGA TGGAACAGTC GGTTGGATCC TGGATGCTAT TGACAAGGCA
    AACCTTCCAT ATAGACCACC TGTAGCTGTG CTGCCCCTCG GTACAGGAAA TGACCTTGCG
    CGATGTCTCA GCTGGGGAGG TGGTTATGAT GGAGAAAATC TAATGAAGTT CTTGAAGGAC
    ATTGAAAAGA GTGTACCCAT TAAAATGGAC AGATGGAACA TTGAGGTTAT ACCTGAAAAC
    CCAGATGAGA AGGGAGACCC AGTTCCTTAC GAGATTATAA ACAACTACTT TTCCATTGGG
    GTGGATGCAT CTATAGCTCA CAGATTCCAT AATATGAGGG AAAAATATCC AGAGAAATTC
    AATAGCAGAA TGAAGAACAA ATTGTGGTAT TTTGAGTTTG CTACATCAGA GACTGTTTTC
    TCTACCTGTA AAAAACTTAA AGAGGTCATT ACAATTGAGT GTTGTGGCTC CCCGTTGAAA
    CTCAGCTCTC TGTCCCTGGA AGGCATCGCA GTTATAAATA TTCCAAGCAT GCATGGGGGT
    TCCAACCTAT GGGGAGAGAC TAAGAAACAC TGGGGAGAAC CTCGCAATGC TTCATGTCAT
    AATGCCCCTG AAGCCATTAC TGACCCTGAT GCCTTAAAGT CATGTGTACA AGACCTCAGT
    GACAAGAGGC TGGAGGTAGT AGGACTTGAG GGTGCTATTG AGATGGGGCA GATTTATACT
    GGACTGAAGA GTGCTGGCAA ACGACTTGCA ACATGTTCTG AACTAACAAT AAGGACCACA
    AAGCCCCTCC CAATGCAAAT TGATGGCGAG CCCTGGATGC AGCCACCCTG CACGATAAAA
    ATCACACATA AGAACCAGGC ACCGATGCTG CGTGCCCCTG CGCCCCGTTC TTCAAGCTTT
    TTCTCCCTGA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa06017
    Clone ID Related Accession (Same CDS sequence) XM_012957810.2 , XM_012957812.2 , XM_012957808.2 , XM_018091693.1 , XM_012957809.2 , XM_018091697.1 , XM_012957811.2 , XM_018091692.1 , XM_012957813.2 , XM_018091694.1 , XM_012957814.2 , XM_018091698.1
    Accession Version XM_012957808.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2235bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product diacylglycerol kinase alpha isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957808.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCGACG AGAACAAGGA GTGGGCAATC CTGAGCCCGG AGGACTTCAC CCAACTGCAG 
    AAGTACATTG ATTACAGCAC CAAGAAGGTT AAAGATGTTC AGCAAGAGTT CTATGGGGAT
    GGTACATCGT GCCCTCACTT GGTTGAAGAC ACAATAGATT TTGAAGCATT CAAATCCTTT
    TTGAAAACAT ACCTGGAAGT AGATGACATT CCCAACAATC TCTGTGAGCG TCTCTTCATG
    TCTTTTCAAG CAACCCCAAT TGAGAGCAAA AAAAGTGGGG AGGATGAGAC AGAAGGCATA
    AAAGGTAGTG GCCGTATCAG TCTAAATGAT GTGTCCTGTT ATTTCTCTTT GCTGGAAAGT
    GGCACTGCTG AGGATAAACT GGCATTCACC TTTAAATTGT ATGACAAAGA CAGCAATGGT
    CTTCTGGACA GCTCTGAGGT GGACAGGATT ATAACACAAA TGATGCGTGT AGCGGAATAT
    CTGGACTGGG ACATCACGGA ACTCAAACCT ATGTTAGAGG AGATGATGAG ACAAGTGGAC
    TATGATGGAA ATGGAATAGT CTCTTATGAA GAATGGGTTA AAGGAGGACT CACAACAGTG
    CCTCTGCTGG TGCTTCTAGG AATGGAAGTT AATATAAAAG ACGATGGGCA GCACCTGTGG
    CGCCTGAAGC ATTTCAACCG CCCTGTGTAT TGCAATGTGT GTGAAGCTCT TCTTTTTGGG
    GTCAGGAAGC AGGGCCTGTG TTGCACTTTC TGTAAGTTTA CCGTTCATGA ACGCTGTGCC
    CGCAAAGCGC CTAATTCCTG CATAAGCACG TATGCCAAAT CACGCAAGGA TTTCAGCGAG
    CAGGCCCATG TTTGGATGAG AGGTGGATGT GAGGCCAGCA AGTGTGACAA ATGCCAAAAA
    AAAATAAAAA GTTTCCAGAG CCTGACTGGG CAGCGCTGTG TGTGGTGTCA TACAAAGGTT
    CATGATGAAT GTGCCTCATC CCCACTGTGT GATGGTGGAA TCTTAAAGGA CCACATATTA
    CCTCCTGCTT GCATATATCC AGTAATCCTG GAACGACAGA ATAGCCAAAA AAATCTTCTA
    GCTTCCTCAG AAGAAGCATC ACAGACGTTT AGTACCCCAG AAGGACAAGT CCTGCGGATT
    GCCCCATTAC CAGGTACACA CCCTCTACTT GTCTTTGTAA ACCCAAAGAG TGGAGGGAAG
    CAGGGGGAAA GAGTGCTGAG GAAATTCCAG TATCTGCTAA ATCCCAGACA AGTCCATAAC
    TTGATAAAGG GAGGCCCTTC CCCAGGGCTG AATTTTTTCC GGGATGTCCC AGAATATCGT
    ATCTTAGTAT GCGGCGGTGA TGGAACAGTC GGTTGGATCC TGGATGCTAT TGACAAGGCA
    AACCTTCCAT ATAGACCACC TGTAGCTGTG CTGCCCCTCG GTACAGGAAA TGACCTTGCG
    CGATGTCTCA GCTGGGGAGG TGGTTATGAT GGAGAAAATC TAATGAAGTT CTTGAAGGAC
    ATTGAAAAGA GTGTACCCAT TAAAATGGAC AGATGGAACA TTGAGGTTAT ACCTGAAAAC
    CCAGATGAGA AGGGAGACCC AGTTCCTTAC GAGATTATAA ACAACTACTT TTCCATTGGG
    GTGGATGCAT CTATAGCTCA CAGATTCCAT AATATGAGGG AAAAATATCC AGAGAAATTC
    AATAGCAGAA TGAAGAACAA ATTGTGGTAT TTTGAGTTTG CTACATCAGA GACTGTTTTC
    TCTACCTGTA AAAAACTTAA AGAGGTCATT ACAATTGAGT GTTGTGGCTC CCCGTTGAAA
    CTCAGCTCTC TGTCCCTGGA AGGCATCGCA GTTATAAATA TTCCAAGCAT GCATGGGGGT
    TCCAACCTAT GGGGAGAGAC TAAGAAACAC TGGGGAGAAC CTCGCAATGC TTCATGTCAT
    AATGCCCCTG AAGCCATTAC TGACCCTGAT GCCTTAAAGT CATGTGTACA AGACCTCAGT
    GACAAGAGGC TGGAGGTAGT AGGACTTGAG GGTGCTATTG AGATGGGGCA GATTTATACT
    GGACTGAAGA GTGCTGGCAA ACGACTTGCA ACATGTTCTG AACTAACAAT AAGGACCACA
    AAGCCCCTCC CAATGCAAAT TGATGGCGAG CCCTGGATGC AGCCACCCTG CACGATAAAA
    ATCACACATA AGAACCAGGC ACCGATGCTG CGTGCCCCTG CGCCCCGTTC TTCAAGCTTT
    TTCTCCCTGA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813262.1
    CDS302..2536
    Translation

    Target ORF information:

    RefSeq Version XM_012957808.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis diacylglycerol kinase alpha (dgka), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957808.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCGACG AGAACAAGGA GTGGGCAATC CTGAGCCCGG AGGACTTCAC CCAACTGCAG 
    AAGTACATTG ATTACAGCAC CAAGAAGGTT AAAGATGTTC AGCAAGAGTT CTATGGGGAT
    GGTACATCGT GCCCTCACTT GGTTGAAGAC ACAATAGATT TTGAAGCATT CAAATCCTTT
    TTGAAAACAT ACCTGGAAGT AGATGACATT CCCAACAATC TCTGTGAGCG TCTCTTCATG
    TCTTTTCAAG CAACCCCAAT TGAGAGCAAA AAAAGTGGGG AGGATGAGAC AGAAGGCATA
    AAAGGTAGTG GCCGTATCAG TCTAAATGAT GTGTCCTGTT ATTTCTCTTT GCTGGAAAGT
    GGCACTGCTG AGGATAAACT GGCATTCACC TTTAAATTGT ATGACAAAGA CAGCAATGGT
    CTTCTGGACA GCTCTGAGGT GGACAGGATT ATAACACAAA TGATGCGTGT AGCGGAATAT
    CTGGACTGGG ACATCACGGA ACTCAAACCT ATGTTAGAGG AGATGATGAG ACAAGTGGAC
    TATGATGGAA ATGGAATAGT CTCTTATGAA GAATGGGTTA AAGGAGGACT CACAACAGTG
    CCTCTGCTGG TGCTTCTAGG AATGGAAGTT AATATAAAAG ACGATGGGCA GCACCTGTGG
    CGCCTGAAGC ATTTCAACCG CCCTGTGTAT TGCAATGTGT GTGAAGCTCT TCTTTTTGGG
    GTCAGGAAGC AGGGCCTGTG TTGCACTTTC TGTAAGTTTA CCGTTCATGA ACGCTGTGCC
    CGCAAAGCGC CTAATTCCTG CATAAGCACG TATGCCAAAT CACGCAAGGA TTTCAGCGAG
    CAGGCCCATG TTTGGATGAG AGGTGGATGT GAGGCCAGCA AGTGTGACAA ATGCCAAAAA
    AAAATAAAAA GTTTCCAGAG CCTGACTGGG CAGCGCTGTG TGTGGTGTCA TACAAAGGTT
    CATGATGAAT GTGCCTCATC CCCACTGTGT GATGGTGGAA TCTTAAAGGA CCACATATTA
    CCTCCTGCTT GCATATATCC AGTAATCCTG GAACGACAGA ATAGCCAAAA AAATCTTCTA
    GCTTCCTCAG AAGAAGCATC ACAGACGTTT AGTACCCCAG AAGGACAAGT CCTGCGGATT
    GCCCCATTAC CAGGTACACA CCCTCTACTT GTCTTTGTAA ACCCAAAGAG TGGAGGGAAG
    CAGGGGGAAA GAGTGCTGAG GAAATTCCAG TATCTGCTAA ATCCCAGACA AGTCCATAAC
    TTGATAAAGG GAGGCCCTTC CCCAGGGCTG AATTTTTTCC GGGATGTCCC AGAATATCGT
    ATCTTAGTAT GCGGCGGTGA TGGAACAGTC GGTTGGATCC TGGATGCTAT TGACAAGGCA
    AACCTTCCAT ATAGACCACC TGTAGCTGTG CTGCCCCTCG GTACAGGAAA TGACCTTGCG
    CGATGTCTCA GCTGGGGAGG TGGTTATGAT GGAGAAAATC TAATGAAGTT CTTGAAGGAC
    ATTGAAAAGA GTGTACCCAT TAAAATGGAC AGATGGAACA TTGAGGTTAT ACCTGAAAAC
    CCAGATGAGA AGGGAGACCC AGTTCCTTAC GAGATTATAA ACAACTACTT TTCCATTGGG
    GTGGATGCAT CTATAGCTCA CAGATTCCAT AATATGAGGG AAAAATATCC AGAGAAATTC
    AATAGCAGAA TGAAGAACAA ATTGTGGTAT TTTGAGTTTG CTACATCAGA GACTGTTTTC
    TCTACCTGTA AAAAACTTAA AGAGGTCATT ACAATTGAGT GTTGTGGCTC CCCGTTGAAA
    CTCAGCTCTC TGTCCCTGGA AGGCATCGCA GTTATAAATA TTCCAAGCAT GCATGGGGGT
    TCCAACCTAT GGGGAGAGAC TAAGAAACAC TGGGGAGAAC CTCGCAATGC TTCATGTCAT
    AATGCCCCTG AAGCCATTAC TGACCCTGAT GCCTTAAAGT CATGTGTACA AGACCTCAGT
    GACAAGAGGC TGGAGGTAGT AGGACTTGAG GGTGCTATTG AGATGGGGCA GATTTATACT
    GGACTGAAGA GTGCTGGCAA ACGACTTGCA ACATGTTCTG AACTAACAAT AAGGACCACA
    AAGCCCCTCC CAATGCAAAT TGATGGCGAG CCCTGGATGC AGCCACCCTG CACGATAAAA
    ATCACACATA AGAACCAGGC ACCGATGCTG CGTGCCCCTG CGCCCCGTTC TTCAAGCTTT
    TTCTCCCTGA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa06017
    Clone ID Related Accession (Same CDS sequence) XM_012957810.2 , XM_012957812.2 , XM_012957808.2 , XM_018091693.1 , XM_012957809.2 , XM_018091697.1 , XM_012957811.2 , XM_018091692.1 , XM_012957813.2 , XM_018091694.1 , XM_012957814.2 , XM_018091698.1
    Accession Version XM_018091693.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2235bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product diacylglycerol kinase alpha isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCGACG AGAACAAGGA GTGGGCAATC CTGAGCCCGG AGGACTTCAC CCAACTGCAG 
    AAGTACATTG ATTACAGCAC CAAGAAGGTT AAAGATGTTC AGCAAGAGTT CTATGGGGAT
    GGTACATCGT GCCCTCACTT GGTTGAAGAC ACAATAGATT TTGAAGCATT CAAATCCTTT
    TTGAAAACAT ACCTGGAAGT AGATGACATT CCCAACAATC TCTGTGAGCG TCTCTTCATG
    TCTTTTCAAG CAACCCCAAT TGAGAGCAAA AAAAGTGGGG AGGATGAGAC AGAAGGCATA
    AAAGGTAGTG GCCGTATCAG TCTAAATGAT GTGTCCTGTT ATTTCTCTTT GCTGGAAAGT
    GGCACTGCTG AGGATAAACT GGCATTCACC TTTAAATTGT ATGACAAAGA CAGCAATGGT
    CTTCTGGACA GCTCTGAGGT GGACAGGATT ATAACACAAA TGATGCGTGT AGCGGAATAT
    CTGGACTGGG ACATCACGGA ACTCAAACCT ATGTTAGAGG AGATGATGAG ACAAGTGGAC
    TATGATGGAA ATGGAATAGT CTCTTATGAA GAATGGGTTA AAGGAGGACT CACAACAGTG
    CCTCTGCTGG TGCTTCTAGG AATGGAAGTT AATATAAAAG ACGATGGGCA GCACCTGTGG
    CGCCTGAAGC ATTTCAACCG CCCTGTGTAT TGCAATGTGT GTGAAGCTCT TCTTTTTGGG
    GTCAGGAAGC AGGGCCTGTG TTGCACTTTC TGTAAGTTTA CCGTTCATGA ACGCTGTGCC
    CGCAAAGCGC CTAATTCCTG CATAAGCACG TATGCCAAAT CACGCAAGGA TTTCAGCGAG
    CAGGCCCATG TTTGGATGAG AGGTGGATGT GAGGCCAGCA AGTGTGACAA ATGCCAAAAA
    AAAATAAAAA GTTTCCAGAG CCTGACTGGG CAGCGCTGTG TGTGGTGTCA TACAAAGGTT
    CATGATGAAT GTGCCTCATC CCCACTGTGT GATGGTGGAA TCTTAAAGGA CCACATATTA
    CCTCCTGCTT GCATATATCC AGTAATCCTG GAACGACAGA ATAGCCAAAA AAATCTTCTA
    GCTTCCTCAG AAGAAGCATC ACAGACGTTT AGTACCCCAG AAGGACAAGT CCTGCGGATT
    GCCCCATTAC CAGGTACACA CCCTCTACTT GTCTTTGTAA ACCCAAAGAG TGGAGGGAAG
    CAGGGGGAAA GAGTGCTGAG GAAATTCCAG TATCTGCTAA ATCCCAGACA AGTCCATAAC
    TTGATAAAGG GAGGCCCTTC CCCAGGGCTG AATTTTTTCC GGGATGTCCC AGAATATCGT
    ATCTTAGTAT GCGGCGGTGA TGGAACAGTC GGTTGGATCC TGGATGCTAT TGACAAGGCA
    AACCTTCCAT ATAGACCACC TGTAGCTGTG CTGCCCCTCG GTACAGGAAA TGACCTTGCG
    CGATGTCTCA GCTGGGGAGG TGGTTATGAT GGAGAAAATC TAATGAAGTT CTTGAAGGAC
    ATTGAAAAGA GTGTACCCAT TAAAATGGAC AGATGGAACA TTGAGGTTAT ACCTGAAAAC
    CCAGATGAGA AGGGAGACCC AGTTCCTTAC GAGATTATAA ACAACTACTT TTCCATTGGG
    GTGGATGCAT CTATAGCTCA CAGATTCCAT AATATGAGGG AAAAATATCC AGAGAAATTC
    AATAGCAGAA TGAAGAACAA ATTGTGGTAT TTTGAGTTTG CTACATCAGA GACTGTTTTC
    TCTACCTGTA AAAAACTTAA AGAGGTCATT ACAATTGAGT GTTGTGGCTC CCCGTTGAAA
    CTCAGCTCTC TGTCCCTGGA AGGCATCGCA GTTATAAATA TTCCAAGCAT GCATGGGGGT
    TCCAACCTAT GGGGAGAGAC TAAGAAACAC TGGGGAGAAC CTCGCAATGC TTCATGTCAT
    AATGCCCCTG AAGCCATTAC TGACCCTGAT GCCTTAAAGT CATGTGTACA AGACCTCAGT
    GACAAGAGGC TGGAGGTAGT AGGACTTGAG GGTGCTATTG AGATGGGGCA GATTTATACT
    GGACTGAAGA GTGCTGGCAA ACGACTTGCA ACATGTTCTG AACTAACAAT AAGGACCACA
    AAGCCCCTCC CAATGCAAAT TGATGGCGAG CCCTGGATGC AGCCACCCTG CACGATAAAA
    ATCACACATA AGAACCAGGC ACCGATGCTG CGTGCCCCTG CGCCCCGTTC TTCAAGCTTT
    TTCTCCCTGA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947182.1
    CDS477..2711
    Translation

    Target ORF information:

    RefSeq Version XM_018091693.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis diacylglycerol kinase alpha (dgka), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091693.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCGACG AGAACAAGGA GTGGGCAATC CTGAGCCCGG AGGACTTCAC CCAACTGCAG 
    AAGTACATTG ATTACAGCAC CAAGAAGGTT AAAGATGTTC AGCAAGAGTT CTATGGGGAT
    GGTACATCGT GCCCTCACTT GGTTGAAGAC ACAATAGATT TTGAAGCATT CAAATCCTTT
    TTGAAAACAT ACCTGGAAGT AGATGACATT CCCAACAATC TCTGTGAGCG TCTCTTCATG
    TCTTTTCAAG CAACCCCAAT TGAGAGCAAA AAAAGTGGGG AGGATGAGAC AGAAGGCATA
    AAAGGTAGTG GCCGTATCAG TCTAAATGAT GTGTCCTGTT ATTTCTCTTT GCTGGAAAGT
    GGCACTGCTG AGGATAAACT GGCATTCACC TTTAAATTGT ATGACAAAGA CAGCAATGGT
    CTTCTGGACA GCTCTGAGGT GGACAGGATT ATAACACAAA TGATGCGTGT AGCGGAATAT
    CTGGACTGGG ACATCACGGA ACTCAAACCT ATGTTAGAGG AGATGATGAG ACAAGTGGAC
    TATGATGGAA ATGGAATAGT CTCTTATGAA GAATGGGTTA AAGGAGGACT CACAACAGTG
    CCTCTGCTGG TGCTTCTAGG AATGGAAGTT AATATAAAAG ACGATGGGCA GCACCTGTGG
    CGCCTGAAGC ATTTCAACCG CCCTGTGTAT TGCAATGTGT GTGAAGCTCT TCTTTTTGGG
    GTCAGGAAGC AGGGCCTGTG TTGCACTTTC TGTAAGTTTA CCGTTCATGA ACGCTGTGCC
    CGCAAAGCGC CTAATTCCTG CATAAGCACG TATGCCAAAT CACGCAAGGA TTTCAGCGAG
    CAGGCCCATG TTTGGATGAG AGGTGGATGT GAGGCCAGCA AGTGTGACAA ATGCCAAAAA
    AAAATAAAAA GTTTCCAGAG CCTGACTGGG CAGCGCTGTG TGTGGTGTCA TACAAAGGTT
    CATGATGAAT GTGCCTCATC CCCACTGTGT GATGGTGGAA TCTTAAAGGA CCACATATTA
    CCTCCTGCTT GCATATATCC AGTAATCCTG GAACGACAGA ATAGCCAAAA AAATCTTCTA
    GCTTCCTCAG AAGAAGCATC ACAGACGTTT AGTACCCCAG AAGGACAAGT CCTGCGGATT
    GCCCCATTAC CAGGTACACA CCCTCTACTT GTCTTTGTAA ACCCAAAGAG TGGAGGGAAG
    CAGGGGGAAA GAGTGCTGAG GAAATTCCAG TATCTGCTAA ATCCCAGACA AGTCCATAAC
    TTGATAAAGG GAGGCCCTTC CCCAGGGCTG AATTTTTTCC GGGATGTCCC AGAATATCGT
    ATCTTAGTAT GCGGCGGTGA TGGAACAGTC GGTTGGATCC TGGATGCTAT TGACAAGGCA
    AACCTTCCAT ATAGACCACC TGTAGCTGTG CTGCCCCTCG GTACAGGAAA TGACCTTGCG
    CGATGTCTCA GCTGGGGAGG TGGTTATGAT GGAGAAAATC TAATGAAGTT CTTGAAGGAC
    ATTGAAAAGA GTGTACCCAT TAAAATGGAC AGATGGAACA TTGAGGTTAT ACCTGAAAAC
    CCAGATGAGA AGGGAGACCC AGTTCCTTAC GAGATTATAA ACAACTACTT TTCCATTGGG
    GTGGATGCAT CTATAGCTCA CAGATTCCAT AATATGAGGG AAAAATATCC AGAGAAATTC
    AATAGCAGAA TGAAGAACAA ATTGTGGTAT TTTGAGTTTG CTACATCAGA GACTGTTTTC
    TCTACCTGTA AAAAACTTAA AGAGGTCATT ACAATTGAGT GTTGTGGCTC CCCGTTGAAA
    CTCAGCTCTC TGTCCCTGGA AGGCATCGCA GTTATAAATA TTCCAAGCAT GCATGGGGGT
    TCCAACCTAT GGGGAGAGAC TAAGAAACAC TGGGGAGAAC CTCGCAATGC TTCATGTCAT
    AATGCCCCTG AAGCCATTAC TGACCCTGAT GCCTTAAAGT CATGTGTACA AGACCTCAGT
    GACAAGAGGC TGGAGGTAGT AGGACTTGAG GGTGCTATTG AGATGGGGCA GATTTATACT
    GGACTGAAGA GTGCTGGCAA ACGACTTGCA ACATGTTCTG AACTAACAAT AAGGACCACA
    AAGCCCCTCC CAATGCAAAT TGATGGCGAG CCCTGGATGC AGCCACCCTG CACGATAAAA
    ATCACACATA AGAACCAGGC ACCGATGCTG CGTGCCCCTG CGCCCCGTTC TTCAAGCTTT
    TTCTCCCTGA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa06017
    Clone ID Related Accession (Same CDS sequence) XM_012957810.2 , XM_012957812.2 , XM_012957808.2 , XM_018091693.1 , XM_012957809.2 , XM_018091697.1 , XM_012957811.2 , XM_018091692.1 , XM_012957813.2 , XM_018091694.1 , XM_012957814.2 , XM_018091698.1
    Accession Version XM_012957809.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2235bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product diacylglycerol kinase alpha isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957809.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCGACG AGAACAAGGA GTGGGCAATC CTGAGCCCGG AGGACTTCAC CCAACTGCAG 
    AAGTACATTG ATTACAGCAC CAAGAAGGTT AAAGATGTTC AGCAAGAGTT CTATGGGGAT
    GGTACATCGT GCCCTCACTT GGTTGAAGAC ACAATAGATT TTGAAGCATT CAAATCCTTT
    TTGAAAACAT ACCTGGAAGT AGATGACATT CCCAACAATC TCTGTGAGCG TCTCTTCATG
    TCTTTTCAAG CAACCCCAAT TGAGAGCAAA AAAAGTGGGG AGGATGAGAC AGAAGGCATA
    AAAGGTAGTG GCCGTATCAG TCTAAATGAT GTGTCCTGTT ATTTCTCTTT GCTGGAAAGT
    GGCACTGCTG AGGATAAACT GGCATTCACC TTTAAATTGT ATGACAAAGA CAGCAATGGT
    CTTCTGGACA GCTCTGAGGT GGACAGGATT ATAACACAAA TGATGCGTGT AGCGGAATAT
    CTGGACTGGG ACATCACGGA ACTCAAACCT ATGTTAGAGG AGATGATGAG ACAAGTGGAC
    TATGATGGAA ATGGAATAGT CTCTTATGAA GAATGGGTTA AAGGAGGACT CACAACAGTG
    CCTCTGCTGG TGCTTCTAGG AATGGAAGTT AATATAAAAG ACGATGGGCA GCACCTGTGG
    CGCCTGAAGC ATTTCAACCG CCCTGTGTAT TGCAATGTGT GTGAAGCTCT TCTTTTTGGG
    GTCAGGAAGC AGGGCCTGTG TTGCACTTTC TGTAAGTTTA CCGTTCATGA ACGCTGTGCC
    CGCAAAGCGC CTAATTCCTG CATAAGCACG TATGCCAAAT CACGCAAGGA TTTCAGCGAG
    CAGGCCCATG TTTGGATGAG AGGTGGATGT GAGGCCAGCA AGTGTGACAA ATGCCAAAAA
    AAAATAAAAA GTTTCCAGAG CCTGACTGGG CAGCGCTGTG TGTGGTGTCA TACAAAGGTT
    CATGATGAAT GTGCCTCATC CCCACTGTGT GATGGTGGAA TCTTAAAGGA CCACATATTA
    CCTCCTGCTT GCATATATCC AGTAATCCTG GAACGACAGA ATAGCCAAAA AAATCTTCTA
    GCTTCCTCAG AAGAAGCATC ACAGACGTTT AGTACCCCAG AAGGACAAGT CCTGCGGATT
    GCCCCATTAC CAGGTACACA CCCTCTACTT GTCTTTGTAA ACCCAAAGAG TGGAGGGAAG
    CAGGGGGAAA GAGTGCTGAG GAAATTCCAG TATCTGCTAA ATCCCAGACA AGTCCATAAC
    TTGATAAAGG GAGGCCCTTC CCCAGGGCTG AATTTTTTCC GGGATGTCCC AGAATATCGT
    ATCTTAGTAT GCGGCGGTGA TGGAACAGTC GGTTGGATCC TGGATGCTAT TGACAAGGCA
    AACCTTCCAT ATAGACCACC TGTAGCTGTG CTGCCCCTCG GTACAGGAAA TGACCTTGCG
    CGATGTCTCA GCTGGGGAGG TGGTTATGAT GGAGAAAATC TAATGAAGTT CTTGAAGGAC
    ATTGAAAAGA GTGTACCCAT TAAAATGGAC AGATGGAACA TTGAGGTTAT ACCTGAAAAC
    CCAGATGAGA AGGGAGACCC AGTTCCTTAC GAGATTATAA ACAACTACTT TTCCATTGGG
    GTGGATGCAT CTATAGCTCA CAGATTCCAT AATATGAGGG AAAAATATCC AGAGAAATTC
    AATAGCAGAA TGAAGAACAA ATTGTGGTAT TTTGAGTTTG CTACATCAGA GACTGTTTTC
    TCTACCTGTA AAAAACTTAA AGAGGTCATT ACAATTGAGT GTTGTGGCTC CCCGTTGAAA
    CTCAGCTCTC TGTCCCTGGA AGGCATCGCA GTTATAAATA TTCCAAGCAT GCATGGGGGT
    TCCAACCTAT GGGGAGAGAC TAAGAAACAC TGGGGAGAAC CTCGCAATGC TTCATGTCAT
    AATGCCCCTG AAGCCATTAC TGACCCTGAT GCCTTAAAGT CATGTGTACA AGACCTCAGT
    GACAAGAGGC TGGAGGTAGT AGGACTTGAG GGTGCTATTG AGATGGGGCA GATTTATACT
    GGACTGAAGA GTGCTGGCAA ACGACTTGCA ACATGTTCTG AACTAACAAT AAGGACCACA
    AAGCCCCTCC CAATGCAAAT TGATGGCGAG CCCTGGATGC AGCCACCCTG CACGATAAAA
    ATCACACATA AGAACCAGGC ACCGATGCTG CGTGCCCCTG CGCCCCGTTC TTCAAGCTTT
    TTCTCCCTGA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813263.1
    CDS328..2562
    Translation

    Target ORF information:

    RefSeq Version XM_012957809.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis diacylglycerol kinase alpha (dgka), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957809.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCGACG AGAACAAGGA GTGGGCAATC CTGAGCCCGG AGGACTTCAC CCAACTGCAG 
    AAGTACATTG ATTACAGCAC CAAGAAGGTT AAAGATGTTC AGCAAGAGTT CTATGGGGAT
    GGTACATCGT GCCCTCACTT GGTTGAAGAC ACAATAGATT TTGAAGCATT CAAATCCTTT
    TTGAAAACAT ACCTGGAAGT AGATGACATT CCCAACAATC TCTGTGAGCG TCTCTTCATG
    TCTTTTCAAG CAACCCCAAT TGAGAGCAAA AAAAGTGGGG AGGATGAGAC AGAAGGCATA
    AAAGGTAGTG GCCGTATCAG TCTAAATGAT GTGTCCTGTT ATTTCTCTTT GCTGGAAAGT
    GGCACTGCTG AGGATAAACT GGCATTCACC TTTAAATTGT ATGACAAAGA CAGCAATGGT
    CTTCTGGACA GCTCTGAGGT GGACAGGATT ATAACACAAA TGATGCGTGT AGCGGAATAT
    CTGGACTGGG ACATCACGGA ACTCAAACCT ATGTTAGAGG AGATGATGAG ACAAGTGGAC
    TATGATGGAA ATGGAATAGT CTCTTATGAA GAATGGGTTA AAGGAGGACT CACAACAGTG
    CCTCTGCTGG TGCTTCTAGG AATGGAAGTT AATATAAAAG ACGATGGGCA GCACCTGTGG
    CGCCTGAAGC ATTTCAACCG CCCTGTGTAT TGCAATGTGT GTGAAGCTCT TCTTTTTGGG
    GTCAGGAAGC AGGGCCTGTG TTGCACTTTC TGTAAGTTTA CCGTTCATGA ACGCTGTGCC
    CGCAAAGCGC CTAATTCCTG CATAAGCACG TATGCCAAAT CACGCAAGGA TTTCAGCGAG
    CAGGCCCATG TTTGGATGAG AGGTGGATGT GAGGCCAGCA AGTGTGACAA ATGCCAAAAA
    AAAATAAAAA GTTTCCAGAG CCTGACTGGG CAGCGCTGTG TGTGGTGTCA TACAAAGGTT
    CATGATGAAT GTGCCTCATC CCCACTGTGT GATGGTGGAA TCTTAAAGGA CCACATATTA
    CCTCCTGCTT GCATATATCC AGTAATCCTG GAACGACAGA ATAGCCAAAA AAATCTTCTA
    GCTTCCTCAG AAGAAGCATC ACAGACGTTT AGTACCCCAG AAGGACAAGT CCTGCGGATT
    GCCCCATTAC CAGGTACACA CCCTCTACTT GTCTTTGTAA ACCCAAAGAG TGGAGGGAAG
    CAGGGGGAAA GAGTGCTGAG GAAATTCCAG TATCTGCTAA ATCCCAGACA AGTCCATAAC
    TTGATAAAGG GAGGCCCTTC CCCAGGGCTG AATTTTTTCC GGGATGTCCC AGAATATCGT
    ATCTTAGTAT GCGGCGGTGA TGGAACAGTC GGTTGGATCC TGGATGCTAT TGACAAGGCA
    AACCTTCCAT ATAGACCACC TGTAGCTGTG CTGCCCCTCG GTACAGGAAA TGACCTTGCG
    CGATGTCTCA GCTGGGGAGG TGGTTATGAT GGAGAAAATC TAATGAAGTT CTTGAAGGAC
    ATTGAAAAGA GTGTACCCAT TAAAATGGAC AGATGGAACA TTGAGGTTAT ACCTGAAAAC
    CCAGATGAGA AGGGAGACCC AGTTCCTTAC GAGATTATAA ACAACTACTT TTCCATTGGG
    GTGGATGCAT CTATAGCTCA CAGATTCCAT AATATGAGGG AAAAATATCC AGAGAAATTC
    AATAGCAGAA TGAAGAACAA ATTGTGGTAT TTTGAGTTTG CTACATCAGA GACTGTTTTC
    TCTACCTGTA AAAAACTTAA AGAGGTCATT ACAATTGAGT GTTGTGGCTC CCCGTTGAAA
    CTCAGCTCTC TGTCCCTGGA AGGCATCGCA GTTATAAATA TTCCAAGCAT GCATGGGGGT
    TCCAACCTAT GGGGAGAGAC TAAGAAACAC TGGGGAGAAC CTCGCAATGC TTCATGTCAT
    AATGCCCCTG AAGCCATTAC TGACCCTGAT GCCTTAAAGT CATGTGTACA AGACCTCAGT
    GACAAGAGGC TGGAGGTAGT AGGACTTGAG GGTGCTATTG AGATGGGGCA GATTTATACT
    GGACTGAAGA GTGCTGGCAA ACGACTTGCA ACATGTTCTG AACTAACAAT AAGGACCACA
    AAGCCCCTCC CAATGCAAAT TGATGGCGAG CCCTGGATGC AGCCACCCTG CACGATAAAA
    ATCACACATA AGAACCAGGC ACCGATGCTG CGTGCCCCTG CGCCCCGTTC TTCAAGCTTT
    TTCTCCCTGA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa06017
    Clone ID Related Accession (Same CDS sequence) XM_012957810.2 , XM_012957812.2 , XM_012957808.2 , XM_018091693.1 , XM_012957809.2 , XM_018091697.1 , XM_012957811.2 , XM_018091692.1 , XM_012957813.2 , XM_018091694.1 , XM_012957814.2 , XM_018091698.1
    Accession Version XM_018091697.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2235bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product diacylglycerol kinase alpha isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCGACG AGAACAAGGA GTGGGCAATC CTGAGCCCGG AGGACTTCAC CCAACTGCAG 
    AAGTACATTG ATTACAGCAC CAAGAAGGTT AAAGATGTTC AGCAAGAGTT CTATGGGGAT
    GGTACATCGT GCCCTCACTT GGTTGAAGAC ACAATAGATT TTGAAGCATT CAAATCCTTT
    TTGAAAACAT ACCTGGAAGT AGATGACATT CCCAACAATC TCTGTGAGCG TCTCTTCATG
    TCTTTTCAAG CAACCCCAAT TGAGAGCAAA AAAAGTGGGG AGGATGAGAC AGAAGGCATA
    AAAGGTAGTG GCCGTATCAG TCTAAATGAT GTGTCCTGTT ATTTCTCTTT GCTGGAAAGT
    GGCACTGCTG AGGATAAACT GGCATTCACC TTTAAATTGT ATGACAAAGA CAGCAATGGT
    CTTCTGGACA GCTCTGAGGT GGACAGGATT ATAACACAAA TGATGCGTGT AGCGGAATAT
    CTGGACTGGG ACATCACGGA ACTCAAACCT ATGTTAGAGG AGATGATGAG ACAAGTGGAC
    TATGATGGAA ATGGAATAGT CTCTTATGAA GAATGGGTTA AAGGAGGACT CACAACAGTG
    CCTCTGCTGG TGCTTCTAGG AATGGAAGTT AATATAAAAG ACGATGGGCA GCACCTGTGG
    CGCCTGAAGC ATTTCAACCG CCCTGTGTAT TGCAATGTGT GTGAAGCTCT TCTTTTTGGG
    GTCAGGAAGC AGGGCCTGTG TTGCACTTTC TGTAAGTTTA CCGTTCATGA ACGCTGTGCC
    CGCAAAGCGC CTAATTCCTG CATAAGCACG TATGCCAAAT CACGCAAGGA TTTCAGCGAG
    CAGGCCCATG TTTGGATGAG AGGTGGATGT GAGGCCAGCA AGTGTGACAA ATGCCAAAAA
    AAAATAAAAA GTTTCCAGAG CCTGACTGGG CAGCGCTGTG TGTGGTGTCA TACAAAGGTT
    CATGATGAAT GTGCCTCATC CCCACTGTGT GATGGTGGAA TCTTAAAGGA CCACATATTA
    CCTCCTGCTT GCATATATCC AGTAATCCTG GAACGACAGA ATAGCCAAAA AAATCTTCTA
    GCTTCCTCAG AAGAAGCATC ACAGACGTTT AGTACCCCAG AAGGACAAGT CCTGCGGATT
    GCCCCATTAC CAGGTACACA CCCTCTACTT GTCTTTGTAA ACCCAAAGAG TGGAGGGAAG
    CAGGGGGAAA GAGTGCTGAG GAAATTCCAG TATCTGCTAA ATCCCAGACA AGTCCATAAC
    TTGATAAAGG GAGGCCCTTC CCCAGGGCTG AATTTTTTCC GGGATGTCCC AGAATATCGT
    ATCTTAGTAT GCGGCGGTGA TGGAACAGTC GGTTGGATCC TGGATGCTAT TGACAAGGCA
    AACCTTCCAT ATAGACCACC TGTAGCTGTG CTGCCCCTCG GTACAGGAAA TGACCTTGCG
    CGATGTCTCA GCTGGGGAGG TGGTTATGAT GGAGAAAATC TAATGAAGTT CTTGAAGGAC
    ATTGAAAAGA GTGTACCCAT TAAAATGGAC AGATGGAACA TTGAGGTTAT ACCTGAAAAC
    CCAGATGAGA AGGGAGACCC AGTTCCTTAC GAGATTATAA ACAACTACTT TTCCATTGGG
    GTGGATGCAT CTATAGCTCA CAGATTCCAT AATATGAGGG AAAAATATCC AGAGAAATTC
    AATAGCAGAA TGAAGAACAA ATTGTGGTAT TTTGAGTTTG CTACATCAGA GACTGTTTTC
    TCTACCTGTA AAAAACTTAA AGAGGTCATT ACAATTGAGT GTTGTGGCTC CCCGTTGAAA
    CTCAGCTCTC TGTCCCTGGA AGGCATCGCA GTTATAAATA TTCCAAGCAT GCATGGGGGT
    TCCAACCTAT GGGGAGAGAC TAAGAAACAC TGGGGAGAAC CTCGCAATGC TTCATGTCAT
    AATGCCCCTG AAGCCATTAC TGACCCTGAT GCCTTAAAGT CATGTGTACA AGACCTCAGT
    GACAAGAGGC TGGAGGTAGT AGGACTTGAG GGTGCTATTG AGATGGGGCA GATTTATACT
    GGACTGAAGA GTGCTGGCAA ACGACTTGCA ACATGTTCTG AACTAACAAT AAGGACCACA
    AAGCCCCTCC CAATGCAAAT TGATGGCGAG CCCTGGATGC AGCCACCCTG CACGATAAAA
    ATCACACATA AGAACCAGGC ACCGATGCTG CGTGCCCCTG CGCCCCGTTC TTCAAGCTTT
    TTCTCCCTGA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947186.1
    CDS186..2420
    Translation

    Target ORF information:

    RefSeq Version XM_018091697.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis diacylglycerol kinase alpha (dgka), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091697.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCGACG AGAACAAGGA GTGGGCAATC CTGAGCCCGG AGGACTTCAC CCAACTGCAG 
    AAGTACATTG ATTACAGCAC CAAGAAGGTT AAAGATGTTC AGCAAGAGTT CTATGGGGAT
    GGTACATCGT GCCCTCACTT GGTTGAAGAC ACAATAGATT TTGAAGCATT CAAATCCTTT
    TTGAAAACAT ACCTGGAAGT AGATGACATT CCCAACAATC TCTGTGAGCG TCTCTTCATG
    TCTTTTCAAG CAACCCCAAT TGAGAGCAAA AAAAGTGGGG AGGATGAGAC AGAAGGCATA
    AAAGGTAGTG GCCGTATCAG TCTAAATGAT GTGTCCTGTT ATTTCTCTTT GCTGGAAAGT
    GGCACTGCTG AGGATAAACT GGCATTCACC TTTAAATTGT ATGACAAAGA CAGCAATGGT
    CTTCTGGACA GCTCTGAGGT GGACAGGATT ATAACACAAA TGATGCGTGT AGCGGAATAT
    CTGGACTGGG ACATCACGGA ACTCAAACCT ATGTTAGAGG AGATGATGAG ACAAGTGGAC
    TATGATGGAA ATGGAATAGT CTCTTATGAA GAATGGGTTA AAGGAGGACT CACAACAGTG
    CCTCTGCTGG TGCTTCTAGG AATGGAAGTT AATATAAAAG ACGATGGGCA GCACCTGTGG
    CGCCTGAAGC ATTTCAACCG CCCTGTGTAT TGCAATGTGT GTGAAGCTCT TCTTTTTGGG
    GTCAGGAAGC AGGGCCTGTG TTGCACTTTC TGTAAGTTTA CCGTTCATGA ACGCTGTGCC
    CGCAAAGCGC CTAATTCCTG CATAAGCACG TATGCCAAAT CACGCAAGGA TTTCAGCGAG
    CAGGCCCATG TTTGGATGAG AGGTGGATGT GAGGCCAGCA AGTGTGACAA ATGCCAAAAA
    AAAATAAAAA GTTTCCAGAG CCTGACTGGG CAGCGCTGTG TGTGGTGTCA TACAAAGGTT
    CATGATGAAT GTGCCTCATC CCCACTGTGT GATGGTGGAA TCTTAAAGGA CCACATATTA
    CCTCCTGCTT GCATATATCC AGTAATCCTG GAACGACAGA ATAGCCAAAA AAATCTTCTA
    GCTTCCTCAG AAGAAGCATC ACAGACGTTT AGTACCCCAG AAGGACAAGT CCTGCGGATT
    GCCCCATTAC CAGGTACACA CCCTCTACTT GTCTTTGTAA ACCCAAAGAG TGGAGGGAAG
    CAGGGGGAAA GAGTGCTGAG GAAATTCCAG TATCTGCTAA ATCCCAGACA AGTCCATAAC
    TTGATAAAGG GAGGCCCTTC CCCAGGGCTG AATTTTTTCC GGGATGTCCC AGAATATCGT
    ATCTTAGTAT GCGGCGGTGA TGGAACAGTC GGTTGGATCC TGGATGCTAT TGACAAGGCA
    AACCTTCCAT ATAGACCACC TGTAGCTGTG CTGCCCCTCG GTACAGGAAA TGACCTTGCG
    CGATGTCTCA GCTGGGGAGG TGGTTATGAT GGAGAAAATC TAATGAAGTT CTTGAAGGAC
    ATTGAAAAGA GTGTACCCAT TAAAATGGAC AGATGGAACA TTGAGGTTAT ACCTGAAAAC
    CCAGATGAGA AGGGAGACCC AGTTCCTTAC GAGATTATAA ACAACTACTT TTCCATTGGG
    GTGGATGCAT CTATAGCTCA CAGATTCCAT AATATGAGGG AAAAATATCC AGAGAAATTC
    AATAGCAGAA TGAAGAACAA ATTGTGGTAT TTTGAGTTTG CTACATCAGA GACTGTTTTC
    TCTACCTGTA AAAAACTTAA AGAGGTCATT ACAATTGAGT GTTGTGGCTC CCCGTTGAAA
    CTCAGCTCTC TGTCCCTGGA AGGCATCGCA GTTATAAATA TTCCAAGCAT GCATGGGGGT
    TCCAACCTAT GGGGAGAGAC TAAGAAACAC TGGGGAGAAC CTCGCAATGC TTCATGTCAT
    AATGCCCCTG AAGCCATTAC TGACCCTGAT GCCTTAAAGT CATGTGTACA AGACCTCAGT
    GACAAGAGGC TGGAGGTAGT AGGACTTGAG GGTGCTATTG AGATGGGGCA GATTTATACT
    GGACTGAAGA GTGCTGGCAA ACGACTTGCA ACATGTTCTG AACTAACAAT AAGGACCACA
    AAGCCCCTCC CAATGCAAAT TGATGGCGAG CCCTGGATGC AGCCACCCTG CACGATAAAA
    ATCACACATA AGAACCAGGC ACCGATGCTG CGTGCCCCTG CGCCCCGTTC TTCAAGCTTT
    TTCTCCCTGA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa06017
    Clone ID Related Accession (Same CDS sequence) XM_012957810.2 , XM_012957812.2 , XM_012957808.2 , XM_018091693.1 , XM_012957809.2 , XM_018091697.1 , XM_012957811.2 , XM_018091692.1 , XM_012957813.2 , XM_018091694.1 , XM_012957814.2 , XM_018091698.1
    Accession Version XM_012957811.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2235bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product diacylglycerol kinase alpha isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957811.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCGACG AGAACAAGGA GTGGGCAATC CTGAGCCCGG AGGACTTCAC CCAACTGCAG 
    AAGTACATTG ATTACAGCAC CAAGAAGGTT AAAGATGTTC AGCAAGAGTT CTATGGGGAT
    GGTACATCGT GCCCTCACTT GGTTGAAGAC ACAATAGATT TTGAAGCATT CAAATCCTTT
    TTGAAAACAT ACCTGGAAGT AGATGACATT CCCAACAATC TCTGTGAGCG TCTCTTCATG
    TCTTTTCAAG CAACCCCAAT TGAGAGCAAA AAAAGTGGGG AGGATGAGAC AGAAGGCATA
    AAAGGTAGTG GCCGTATCAG TCTAAATGAT GTGTCCTGTT ATTTCTCTTT GCTGGAAAGT
    GGCACTGCTG AGGATAAACT GGCATTCACC TTTAAATTGT ATGACAAAGA CAGCAATGGT
    CTTCTGGACA GCTCTGAGGT GGACAGGATT ATAACACAAA TGATGCGTGT AGCGGAATAT
    CTGGACTGGG ACATCACGGA ACTCAAACCT ATGTTAGAGG AGATGATGAG ACAAGTGGAC
    TATGATGGAA ATGGAATAGT CTCTTATGAA GAATGGGTTA AAGGAGGACT CACAACAGTG
    CCTCTGCTGG TGCTTCTAGG AATGGAAGTT AATATAAAAG ACGATGGGCA GCACCTGTGG
    CGCCTGAAGC ATTTCAACCG CCCTGTGTAT TGCAATGTGT GTGAAGCTCT TCTTTTTGGG
    GTCAGGAAGC AGGGCCTGTG TTGCACTTTC TGTAAGTTTA CCGTTCATGA ACGCTGTGCC
    CGCAAAGCGC CTAATTCCTG CATAAGCACG TATGCCAAAT CACGCAAGGA TTTCAGCGAG
    CAGGCCCATG TTTGGATGAG AGGTGGATGT GAGGCCAGCA AGTGTGACAA ATGCCAAAAA
    AAAATAAAAA GTTTCCAGAG CCTGACTGGG CAGCGCTGTG TGTGGTGTCA TACAAAGGTT
    CATGATGAAT GTGCCTCATC CCCACTGTGT GATGGTGGAA TCTTAAAGGA CCACATATTA
    CCTCCTGCTT GCATATATCC AGTAATCCTG GAACGACAGA ATAGCCAAAA AAATCTTCTA
    GCTTCCTCAG AAGAAGCATC ACAGACGTTT AGTACCCCAG AAGGACAAGT CCTGCGGATT
    GCCCCATTAC CAGGTACACA CCCTCTACTT GTCTTTGTAA ACCCAAAGAG TGGAGGGAAG
    CAGGGGGAAA GAGTGCTGAG GAAATTCCAG TATCTGCTAA ATCCCAGACA AGTCCATAAC
    TTGATAAAGG GAGGCCCTTC CCCAGGGCTG AATTTTTTCC GGGATGTCCC AGAATATCGT
    ATCTTAGTAT GCGGCGGTGA TGGAACAGTC GGTTGGATCC TGGATGCTAT TGACAAGGCA
    AACCTTCCAT ATAGACCACC TGTAGCTGTG CTGCCCCTCG GTACAGGAAA TGACCTTGCG
    CGATGTCTCA GCTGGGGAGG TGGTTATGAT GGAGAAAATC TAATGAAGTT CTTGAAGGAC
    ATTGAAAAGA GTGTACCCAT TAAAATGGAC AGATGGAACA TTGAGGTTAT ACCTGAAAAC
    CCAGATGAGA AGGGAGACCC AGTTCCTTAC GAGATTATAA ACAACTACTT TTCCATTGGG
    GTGGATGCAT CTATAGCTCA CAGATTCCAT AATATGAGGG AAAAATATCC AGAGAAATTC
    AATAGCAGAA TGAAGAACAA ATTGTGGTAT TTTGAGTTTG CTACATCAGA GACTGTTTTC
    TCTACCTGTA AAAAACTTAA AGAGGTCATT ACAATTGAGT GTTGTGGCTC CCCGTTGAAA
    CTCAGCTCTC TGTCCCTGGA AGGCATCGCA GTTATAAATA TTCCAAGCAT GCATGGGGGT
    TCCAACCTAT GGGGAGAGAC TAAGAAACAC TGGGGAGAAC CTCGCAATGC TTCATGTCAT
    AATGCCCCTG AAGCCATTAC TGACCCTGAT GCCTTAAAGT CATGTGTACA AGACCTCAGT
    GACAAGAGGC TGGAGGTAGT AGGACTTGAG GGTGCTATTG AGATGGGGCA GATTTATACT
    GGACTGAAGA GTGCTGGCAA ACGACTTGCA ACATGTTCTG AACTAACAAT AAGGACCACA
    AAGCCCCTCC CAATGCAAAT TGATGGCGAG CCCTGGATGC AGCCACCCTG CACGATAAAA
    ATCACACATA AGAACCAGGC ACCGATGCTG CGTGCCCCTG CGCCCCGTTC TTCAAGCTTT
    TTCTCCCTGA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813265.1
    CDS431..2665
    Translation

    Target ORF information:

    RefSeq Version XM_012957811.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis diacylglycerol kinase alpha (dgka), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957811.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCGACG AGAACAAGGA GTGGGCAATC CTGAGCCCGG AGGACTTCAC CCAACTGCAG 
    AAGTACATTG ATTACAGCAC CAAGAAGGTT AAAGATGTTC AGCAAGAGTT CTATGGGGAT
    GGTACATCGT GCCCTCACTT GGTTGAAGAC ACAATAGATT TTGAAGCATT CAAATCCTTT
    TTGAAAACAT ACCTGGAAGT AGATGACATT CCCAACAATC TCTGTGAGCG TCTCTTCATG
    TCTTTTCAAG CAACCCCAAT TGAGAGCAAA AAAAGTGGGG AGGATGAGAC AGAAGGCATA
    AAAGGTAGTG GCCGTATCAG TCTAAATGAT GTGTCCTGTT ATTTCTCTTT GCTGGAAAGT
    GGCACTGCTG AGGATAAACT GGCATTCACC TTTAAATTGT ATGACAAAGA CAGCAATGGT
    CTTCTGGACA GCTCTGAGGT GGACAGGATT ATAACACAAA TGATGCGTGT AGCGGAATAT
    CTGGACTGGG ACATCACGGA ACTCAAACCT ATGTTAGAGG AGATGATGAG ACAAGTGGAC
    TATGATGGAA ATGGAATAGT CTCTTATGAA GAATGGGTTA AAGGAGGACT CACAACAGTG
    CCTCTGCTGG TGCTTCTAGG AATGGAAGTT AATATAAAAG ACGATGGGCA GCACCTGTGG
    CGCCTGAAGC ATTTCAACCG CCCTGTGTAT TGCAATGTGT GTGAAGCTCT TCTTTTTGGG
    GTCAGGAAGC AGGGCCTGTG TTGCACTTTC TGTAAGTTTA CCGTTCATGA ACGCTGTGCC
    CGCAAAGCGC CTAATTCCTG CATAAGCACG TATGCCAAAT CACGCAAGGA TTTCAGCGAG
    CAGGCCCATG TTTGGATGAG AGGTGGATGT GAGGCCAGCA AGTGTGACAA ATGCCAAAAA
    AAAATAAAAA GTTTCCAGAG CCTGACTGGG CAGCGCTGTG TGTGGTGTCA TACAAAGGTT
    CATGATGAAT GTGCCTCATC CCCACTGTGT GATGGTGGAA TCTTAAAGGA CCACATATTA
    CCTCCTGCTT GCATATATCC AGTAATCCTG GAACGACAGA ATAGCCAAAA AAATCTTCTA
    GCTTCCTCAG AAGAAGCATC ACAGACGTTT AGTACCCCAG AAGGACAAGT CCTGCGGATT
    GCCCCATTAC CAGGTACACA CCCTCTACTT GTCTTTGTAA ACCCAAAGAG TGGAGGGAAG
    CAGGGGGAAA GAGTGCTGAG GAAATTCCAG TATCTGCTAA ATCCCAGACA AGTCCATAAC
    TTGATAAAGG GAGGCCCTTC CCCAGGGCTG AATTTTTTCC GGGATGTCCC AGAATATCGT
    ATCTTAGTAT GCGGCGGTGA TGGAACAGTC GGTTGGATCC TGGATGCTAT TGACAAGGCA
    AACCTTCCAT ATAGACCACC TGTAGCTGTG CTGCCCCTCG GTACAGGAAA TGACCTTGCG
    CGATGTCTCA GCTGGGGAGG TGGTTATGAT GGAGAAAATC TAATGAAGTT CTTGAAGGAC
    ATTGAAAAGA GTGTACCCAT TAAAATGGAC AGATGGAACA TTGAGGTTAT ACCTGAAAAC
    CCAGATGAGA AGGGAGACCC AGTTCCTTAC GAGATTATAA ACAACTACTT TTCCATTGGG
    GTGGATGCAT CTATAGCTCA CAGATTCCAT AATATGAGGG AAAAATATCC AGAGAAATTC
    AATAGCAGAA TGAAGAACAA ATTGTGGTAT TTTGAGTTTG CTACATCAGA GACTGTTTTC
    TCTACCTGTA AAAAACTTAA AGAGGTCATT ACAATTGAGT GTTGTGGCTC CCCGTTGAAA
    CTCAGCTCTC TGTCCCTGGA AGGCATCGCA GTTATAAATA TTCCAAGCAT GCATGGGGGT
    TCCAACCTAT GGGGAGAGAC TAAGAAACAC TGGGGAGAAC CTCGCAATGC TTCATGTCAT
    AATGCCCCTG AAGCCATTAC TGACCCTGAT GCCTTAAAGT CATGTGTACA AGACCTCAGT
    GACAAGAGGC TGGAGGTAGT AGGACTTGAG GGTGCTATTG AGATGGGGCA GATTTATACT
    GGACTGAAGA GTGCTGGCAA ACGACTTGCA ACATGTTCTG AACTAACAAT AAGGACCACA
    AAGCCCCTCC CAATGCAAAT TGATGGCGAG CCCTGGATGC AGCCACCCTG CACGATAAAA
    ATCACACATA AGAACCAGGC ACCGATGCTG CGTGCCCCTG CGCCCCGTTC TTCAAGCTTT
    TTCTCCCTGA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa06017
    Clone ID Related Accession (Same CDS sequence) XM_012957810.2 , XM_012957812.2 , XM_012957808.2 , XM_018091693.1 , XM_012957809.2 , XM_018091697.1 , XM_012957811.2 , XM_018091692.1 , XM_012957813.2 , XM_018091694.1 , XM_012957814.2 , XM_018091698.1
    Accession Version XM_018091692.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2235bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product diacylglycerol kinase alpha isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCGACG AGAACAAGGA GTGGGCAATC CTGAGCCCGG AGGACTTCAC CCAACTGCAG 
    AAGTACATTG ATTACAGCAC CAAGAAGGTT AAAGATGTTC AGCAAGAGTT CTATGGGGAT
    GGTACATCGT GCCCTCACTT GGTTGAAGAC ACAATAGATT TTGAAGCATT CAAATCCTTT
    TTGAAAACAT ACCTGGAAGT AGATGACATT CCCAACAATC TCTGTGAGCG TCTCTTCATG
    TCTTTTCAAG CAACCCCAAT TGAGAGCAAA AAAAGTGGGG AGGATGAGAC AGAAGGCATA
    AAAGGTAGTG GCCGTATCAG TCTAAATGAT GTGTCCTGTT ATTTCTCTTT GCTGGAAAGT
    GGCACTGCTG AGGATAAACT GGCATTCACC TTTAAATTGT ATGACAAAGA CAGCAATGGT
    CTTCTGGACA GCTCTGAGGT GGACAGGATT ATAACACAAA TGATGCGTGT AGCGGAATAT
    CTGGACTGGG ACATCACGGA ACTCAAACCT ATGTTAGAGG AGATGATGAG ACAAGTGGAC
    TATGATGGAA ATGGAATAGT CTCTTATGAA GAATGGGTTA AAGGAGGACT CACAACAGTG
    CCTCTGCTGG TGCTTCTAGG AATGGAAGTT AATATAAAAG ACGATGGGCA GCACCTGTGG
    CGCCTGAAGC ATTTCAACCG CCCTGTGTAT TGCAATGTGT GTGAAGCTCT TCTTTTTGGG
    GTCAGGAAGC AGGGCCTGTG TTGCACTTTC TGTAAGTTTA CCGTTCATGA ACGCTGTGCC
    CGCAAAGCGC CTAATTCCTG CATAAGCACG TATGCCAAAT CACGCAAGGA TTTCAGCGAG
    CAGGCCCATG TTTGGATGAG AGGTGGATGT GAGGCCAGCA AGTGTGACAA ATGCCAAAAA
    AAAATAAAAA GTTTCCAGAG CCTGACTGGG CAGCGCTGTG TGTGGTGTCA TACAAAGGTT
    CATGATGAAT GTGCCTCATC CCCACTGTGT GATGGTGGAA TCTTAAAGGA CCACATATTA
    CCTCCTGCTT GCATATATCC AGTAATCCTG GAACGACAGA ATAGCCAAAA AAATCTTCTA
    GCTTCCTCAG AAGAAGCATC ACAGACGTTT AGTACCCCAG AAGGACAAGT CCTGCGGATT
    GCCCCATTAC CAGGTACACA CCCTCTACTT GTCTTTGTAA ACCCAAAGAG TGGAGGGAAG
    CAGGGGGAAA GAGTGCTGAG GAAATTCCAG TATCTGCTAA ATCCCAGACA AGTCCATAAC
    TTGATAAAGG GAGGCCCTTC CCCAGGGCTG AATTTTTTCC GGGATGTCCC AGAATATCGT
    ATCTTAGTAT GCGGCGGTGA TGGAACAGTC GGTTGGATCC TGGATGCTAT TGACAAGGCA
    AACCTTCCAT ATAGACCACC TGTAGCTGTG CTGCCCCTCG GTACAGGAAA TGACCTTGCG
    CGATGTCTCA GCTGGGGAGG TGGTTATGAT GGAGAAAATC TAATGAAGTT CTTGAAGGAC
    ATTGAAAAGA GTGTACCCAT TAAAATGGAC AGATGGAACA TTGAGGTTAT ACCTGAAAAC
    CCAGATGAGA AGGGAGACCC AGTTCCTTAC GAGATTATAA ACAACTACTT TTCCATTGGG
    GTGGATGCAT CTATAGCTCA CAGATTCCAT AATATGAGGG AAAAATATCC AGAGAAATTC
    AATAGCAGAA TGAAGAACAA ATTGTGGTAT TTTGAGTTTG CTACATCAGA GACTGTTTTC
    TCTACCTGTA AAAAACTTAA AGAGGTCATT ACAATTGAGT GTTGTGGCTC CCCGTTGAAA
    CTCAGCTCTC TGTCCCTGGA AGGCATCGCA GTTATAAATA TTCCAAGCAT GCATGGGGGT
    TCCAACCTAT GGGGAGAGAC TAAGAAACAC TGGGGAGAAC CTCGCAATGC TTCATGTCAT
    AATGCCCCTG AAGCCATTAC TGACCCTGAT GCCTTAAAGT CATGTGTACA AGACCTCAGT
    GACAAGAGGC TGGAGGTAGT AGGACTTGAG GGTGCTATTG AGATGGGGCA GATTTATACT
    GGACTGAAGA GTGCTGGCAA ACGACTTGCA ACATGTTCTG AACTAACAAT AAGGACCACA
    AAGCCCCTCC CAATGCAAAT TGATGGCGAG CCCTGGATGC AGCCACCCTG CACGATAAAA
    ATCACACATA AGAACCAGGC ACCGATGCTG CGTGCCCCTG CGCCCCGTTC TTCAAGCTTT
    TTCTCCCTGA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947181.1
    CDS503..2737
    Translation

    Target ORF information:

    RefSeq Version XM_018091692.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis diacylglycerol kinase alpha (dgka), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091692.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCGACG AGAACAAGGA GTGGGCAATC CTGAGCCCGG AGGACTTCAC CCAACTGCAG 
    AAGTACATTG ATTACAGCAC CAAGAAGGTT AAAGATGTTC AGCAAGAGTT CTATGGGGAT
    GGTACATCGT GCCCTCACTT GGTTGAAGAC ACAATAGATT TTGAAGCATT CAAATCCTTT
    TTGAAAACAT ACCTGGAAGT AGATGACATT CCCAACAATC TCTGTGAGCG TCTCTTCATG
    TCTTTTCAAG CAACCCCAAT TGAGAGCAAA AAAAGTGGGG AGGATGAGAC AGAAGGCATA
    AAAGGTAGTG GCCGTATCAG TCTAAATGAT GTGTCCTGTT ATTTCTCTTT GCTGGAAAGT
    GGCACTGCTG AGGATAAACT GGCATTCACC TTTAAATTGT ATGACAAAGA CAGCAATGGT
    CTTCTGGACA GCTCTGAGGT GGACAGGATT ATAACACAAA TGATGCGTGT AGCGGAATAT
    CTGGACTGGG ACATCACGGA ACTCAAACCT ATGTTAGAGG AGATGATGAG ACAAGTGGAC
    TATGATGGAA ATGGAATAGT CTCTTATGAA GAATGGGTTA AAGGAGGACT CACAACAGTG
    CCTCTGCTGG TGCTTCTAGG AATGGAAGTT AATATAAAAG ACGATGGGCA GCACCTGTGG
    CGCCTGAAGC ATTTCAACCG CCCTGTGTAT TGCAATGTGT GTGAAGCTCT TCTTTTTGGG
    GTCAGGAAGC AGGGCCTGTG TTGCACTTTC TGTAAGTTTA CCGTTCATGA ACGCTGTGCC
    CGCAAAGCGC CTAATTCCTG CATAAGCACG TATGCCAAAT CACGCAAGGA TTTCAGCGAG
    CAGGCCCATG TTTGGATGAG AGGTGGATGT GAGGCCAGCA AGTGTGACAA ATGCCAAAAA
    AAAATAAAAA GTTTCCAGAG CCTGACTGGG CAGCGCTGTG TGTGGTGTCA TACAAAGGTT
    CATGATGAAT GTGCCTCATC CCCACTGTGT GATGGTGGAA TCTTAAAGGA CCACATATTA
    CCTCCTGCTT GCATATATCC AGTAATCCTG GAACGACAGA ATAGCCAAAA AAATCTTCTA
    GCTTCCTCAG AAGAAGCATC ACAGACGTTT AGTACCCCAG AAGGACAAGT CCTGCGGATT
    GCCCCATTAC CAGGTACACA CCCTCTACTT GTCTTTGTAA ACCCAAAGAG TGGAGGGAAG
    CAGGGGGAAA GAGTGCTGAG GAAATTCCAG TATCTGCTAA ATCCCAGACA AGTCCATAAC
    TTGATAAAGG GAGGCCCTTC CCCAGGGCTG AATTTTTTCC GGGATGTCCC AGAATATCGT
    ATCTTAGTAT GCGGCGGTGA TGGAACAGTC GGTTGGATCC TGGATGCTAT TGACAAGGCA
    AACCTTCCAT ATAGACCACC TGTAGCTGTG CTGCCCCTCG GTACAGGAAA TGACCTTGCG
    CGATGTCTCA GCTGGGGAGG TGGTTATGAT GGAGAAAATC TAATGAAGTT CTTGAAGGAC
    ATTGAAAAGA GTGTACCCAT TAAAATGGAC AGATGGAACA TTGAGGTTAT ACCTGAAAAC
    CCAGATGAGA AGGGAGACCC AGTTCCTTAC GAGATTATAA ACAACTACTT TTCCATTGGG
    GTGGATGCAT CTATAGCTCA CAGATTCCAT AATATGAGGG AAAAATATCC AGAGAAATTC
    AATAGCAGAA TGAAGAACAA ATTGTGGTAT TTTGAGTTTG CTACATCAGA GACTGTTTTC
    TCTACCTGTA AAAAACTTAA AGAGGTCATT ACAATTGAGT GTTGTGGCTC CCCGTTGAAA
    CTCAGCTCTC TGTCCCTGGA AGGCATCGCA GTTATAAATA TTCCAAGCAT GCATGGGGGT
    TCCAACCTAT GGGGAGAGAC TAAGAAACAC TGGGGAGAAC CTCGCAATGC TTCATGTCAT
    AATGCCCCTG AAGCCATTAC TGACCCTGAT GCCTTAAAGT CATGTGTACA AGACCTCAGT
    GACAAGAGGC TGGAGGTAGT AGGACTTGAG GGTGCTATTG AGATGGGGCA GATTTATACT
    GGACTGAAGA GTGCTGGCAA ACGACTTGCA ACATGTTCTG AACTAACAAT AAGGACCACA
    AAGCCCCTCC CAATGCAAAT TGATGGCGAG CCCTGGATGC AGCCACCCTG CACGATAAAA
    ATCACACATA AGAACCAGGC ACCGATGCTG CGTGCCCCTG CGCCCCGTTC TTCAAGCTTT
    TTCTCCCTGA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa06017
    Clone ID Related Accession (Same CDS sequence) XM_012957810.2 , XM_012957812.2 , XM_012957808.2 , XM_018091693.1 , XM_012957809.2 , XM_018091697.1 , XM_012957811.2 , XM_018091692.1 , XM_012957813.2 , XM_018091694.1 , XM_012957814.2 , XM_018091698.1
    Accession Version XM_012957813.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2235bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product diacylglycerol kinase alpha isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957813.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCGACG AGAACAAGGA GTGGGCAATC CTGAGCCCGG AGGACTTCAC CCAACTGCAG 
    AAGTACATTG ATTACAGCAC CAAGAAGGTT AAAGATGTTC AGCAAGAGTT CTATGGGGAT
    GGTACATCGT GCCCTCACTT GGTTGAAGAC ACAATAGATT TTGAAGCATT CAAATCCTTT
    TTGAAAACAT ACCTGGAAGT AGATGACATT CCCAACAATC TCTGTGAGCG TCTCTTCATG
    TCTTTTCAAG CAACCCCAAT TGAGAGCAAA AAAAGTGGGG AGGATGAGAC AGAAGGCATA
    AAAGGTAGTG GCCGTATCAG TCTAAATGAT GTGTCCTGTT ATTTCTCTTT GCTGGAAAGT
    GGCACTGCTG AGGATAAACT GGCATTCACC TTTAAATTGT ATGACAAAGA CAGCAATGGT
    CTTCTGGACA GCTCTGAGGT GGACAGGATT ATAACACAAA TGATGCGTGT AGCGGAATAT
    CTGGACTGGG ACATCACGGA ACTCAAACCT ATGTTAGAGG AGATGATGAG ACAAGTGGAC
    TATGATGGAA ATGGAATAGT CTCTTATGAA GAATGGGTTA AAGGAGGACT CACAACAGTG
    CCTCTGCTGG TGCTTCTAGG AATGGAAGTT AATATAAAAG ACGATGGGCA GCACCTGTGG
    CGCCTGAAGC ATTTCAACCG CCCTGTGTAT TGCAATGTGT GTGAAGCTCT TCTTTTTGGG
    GTCAGGAAGC AGGGCCTGTG TTGCACTTTC TGTAAGTTTA CCGTTCATGA ACGCTGTGCC
    CGCAAAGCGC CTAATTCCTG CATAAGCACG TATGCCAAAT CACGCAAGGA TTTCAGCGAG
    CAGGCCCATG TTTGGATGAG AGGTGGATGT GAGGCCAGCA AGTGTGACAA ATGCCAAAAA
    AAAATAAAAA GTTTCCAGAG CCTGACTGGG CAGCGCTGTG TGTGGTGTCA TACAAAGGTT
    CATGATGAAT GTGCCTCATC CCCACTGTGT GATGGTGGAA TCTTAAAGGA CCACATATTA
    CCTCCTGCTT GCATATATCC AGTAATCCTG GAACGACAGA ATAGCCAAAA AAATCTTCTA
    GCTTCCTCAG AAGAAGCATC ACAGACGTTT AGTACCCCAG AAGGACAAGT CCTGCGGATT
    GCCCCATTAC CAGGTACACA CCCTCTACTT GTCTTTGTAA ACCCAAAGAG TGGAGGGAAG
    CAGGGGGAAA GAGTGCTGAG GAAATTCCAG TATCTGCTAA ATCCCAGACA AGTCCATAAC
    TTGATAAAGG GAGGCCCTTC CCCAGGGCTG AATTTTTTCC GGGATGTCCC AGAATATCGT
    ATCTTAGTAT GCGGCGGTGA TGGAACAGTC GGTTGGATCC TGGATGCTAT TGACAAGGCA
    AACCTTCCAT ATAGACCACC TGTAGCTGTG CTGCCCCTCG GTACAGGAAA TGACCTTGCG
    CGATGTCTCA GCTGGGGAGG TGGTTATGAT GGAGAAAATC TAATGAAGTT CTTGAAGGAC
    ATTGAAAAGA GTGTACCCAT TAAAATGGAC AGATGGAACA TTGAGGTTAT ACCTGAAAAC
    CCAGATGAGA AGGGAGACCC AGTTCCTTAC GAGATTATAA ACAACTACTT TTCCATTGGG
    GTGGATGCAT CTATAGCTCA CAGATTCCAT AATATGAGGG AAAAATATCC AGAGAAATTC
    AATAGCAGAA TGAAGAACAA ATTGTGGTAT TTTGAGTTTG CTACATCAGA GACTGTTTTC
    TCTACCTGTA AAAAACTTAA AGAGGTCATT ACAATTGAGT GTTGTGGCTC CCCGTTGAAA
    CTCAGCTCTC TGTCCCTGGA AGGCATCGCA GTTATAAATA TTCCAAGCAT GCATGGGGGT
    TCCAACCTAT GGGGAGAGAC TAAGAAACAC TGGGGAGAAC CTCGCAATGC TTCATGTCAT
    AATGCCCCTG AAGCCATTAC TGACCCTGAT GCCTTAAAGT CATGTGTACA AGACCTCAGT
    GACAAGAGGC TGGAGGTAGT AGGACTTGAG GGTGCTATTG AGATGGGGCA GATTTATACT
    GGACTGAAGA GTGCTGGCAA ACGACTTGCA ACATGTTCTG AACTAACAAT AAGGACCACA
    AAGCCCCTCC CAATGCAAAT TGATGGCGAG CCCTGGATGC AGCCACCCTG CACGATAAAA
    ATCACACATA AGAACCAGGC ACCGATGCTG CGTGCCCCTG CGCCCCGTTC TTCAAGCTTT
    TTCTCCCTGA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813267.1
    CDS606..2840
    Translation

    Target ORF information:

    RefSeq Version XM_012957813.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis diacylglycerol kinase alpha (dgka), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957813.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCGACG AGAACAAGGA GTGGGCAATC CTGAGCCCGG AGGACTTCAC CCAACTGCAG 
    AAGTACATTG ATTACAGCAC CAAGAAGGTT AAAGATGTTC AGCAAGAGTT CTATGGGGAT
    GGTACATCGT GCCCTCACTT GGTTGAAGAC ACAATAGATT TTGAAGCATT CAAATCCTTT
    TTGAAAACAT ACCTGGAAGT AGATGACATT CCCAACAATC TCTGTGAGCG TCTCTTCATG
    TCTTTTCAAG CAACCCCAAT TGAGAGCAAA AAAAGTGGGG AGGATGAGAC AGAAGGCATA
    AAAGGTAGTG GCCGTATCAG TCTAAATGAT GTGTCCTGTT ATTTCTCTTT GCTGGAAAGT
    GGCACTGCTG AGGATAAACT GGCATTCACC TTTAAATTGT ATGACAAAGA CAGCAATGGT
    CTTCTGGACA GCTCTGAGGT GGACAGGATT ATAACACAAA TGATGCGTGT AGCGGAATAT
    CTGGACTGGG ACATCACGGA ACTCAAACCT ATGTTAGAGG AGATGATGAG ACAAGTGGAC
    TATGATGGAA ATGGAATAGT CTCTTATGAA GAATGGGTTA AAGGAGGACT CACAACAGTG
    CCTCTGCTGG TGCTTCTAGG AATGGAAGTT AATATAAAAG ACGATGGGCA GCACCTGTGG
    CGCCTGAAGC ATTTCAACCG CCCTGTGTAT TGCAATGTGT GTGAAGCTCT TCTTTTTGGG
    GTCAGGAAGC AGGGCCTGTG TTGCACTTTC TGTAAGTTTA CCGTTCATGA ACGCTGTGCC
    CGCAAAGCGC CTAATTCCTG CATAAGCACG TATGCCAAAT CACGCAAGGA TTTCAGCGAG
    CAGGCCCATG TTTGGATGAG AGGTGGATGT GAGGCCAGCA AGTGTGACAA ATGCCAAAAA
    AAAATAAAAA GTTTCCAGAG CCTGACTGGG CAGCGCTGTG TGTGGTGTCA TACAAAGGTT
    CATGATGAAT GTGCCTCATC CCCACTGTGT GATGGTGGAA TCTTAAAGGA CCACATATTA
    CCTCCTGCTT GCATATATCC AGTAATCCTG GAACGACAGA ATAGCCAAAA AAATCTTCTA
    GCTTCCTCAG AAGAAGCATC ACAGACGTTT AGTACCCCAG AAGGACAAGT CCTGCGGATT
    GCCCCATTAC CAGGTACACA CCCTCTACTT GTCTTTGTAA ACCCAAAGAG TGGAGGGAAG
    CAGGGGGAAA GAGTGCTGAG GAAATTCCAG TATCTGCTAA ATCCCAGACA AGTCCATAAC
    TTGATAAAGG GAGGCCCTTC CCCAGGGCTG AATTTTTTCC GGGATGTCCC AGAATATCGT
    ATCTTAGTAT GCGGCGGTGA TGGAACAGTC GGTTGGATCC TGGATGCTAT TGACAAGGCA
    AACCTTCCAT ATAGACCACC TGTAGCTGTG CTGCCCCTCG GTACAGGAAA TGACCTTGCG
    CGATGTCTCA GCTGGGGAGG TGGTTATGAT GGAGAAAATC TAATGAAGTT CTTGAAGGAC
    ATTGAAAAGA GTGTACCCAT TAAAATGGAC AGATGGAACA TTGAGGTTAT ACCTGAAAAC
    CCAGATGAGA AGGGAGACCC AGTTCCTTAC GAGATTATAA ACAACTACTT TTCCATTGGG
    GTGGATGCAT CTATAGCTCA CAGATTCCAT AATATGAGGG AAAAATATCC AGAGAAATTC
    AATAGCAGAA TGAAGAACAA ATTGTGGTAT TTTGAGTTTG CTACATCAGA GACTGTTTTC
    TCTACCTGTA AAAAACTTAA AGAGGTCATT ACAATTGAGT GTTGTGGCTC CCCGTTGAAA
    CTCAGCTCTC TGTCCCTGGA AGGCATCGCA GTTATAAATA TTCCAAGCAT GCATGGGGGT
    TCCAACCTAT GGGGAGAGAC TAAGAAACAC TGGGGAGAAC CTCGCAATGC TTCATGTCAT
    AATGCCCCTG AAGCCATTAC TGACCCTGAT GCCTTAAAGT CATGTGTACA AGACCTCAGT
    GACAAGAGGC TGGAGGTAGT AGGACTTGAG GGTGCTATTG AGATGGGGCA GATTTATACT
    GGACTGAAGA GTGCTGGCAA ACGACTTGCA ACATGTTCTG AACTAACAAT AAGGACCACA
    AAGCCCCTCC CAATGCAAAT TGATGGCGAG CCCTGGATGC AGCCACCCTG CACGATAAAA
    ATCACACATA AGAACCAGGC ACCGATGCTG CGTGCCCCTG CGCCCCGTTC TTCAAGCTTT
    TTCTCCCTGA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa06017
    Clone ID Related Accession (Same CDS sequence) XM_012957810.2 , XM_012957812.2 , XM_012957808.2 , XM_018091693.1 , XM_012957809.2 , XM_018091697.1 , XM_012957811.2 , XM_018091692.1 , XM_012957813.2 , XM_018091694.1 , XM_012957814.2 , XM_018091698.1
    Accession Version XM_018091694.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2235bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product diacylglycerol kinase alpha isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCGACG AGAACAAGGA GTGGGCAATC CTGAGCCCGG AGGACTTCAC CCAACTGCAG 
    AAGTACATTG ATTACAGCAC CAAGAAGGTT AAAGATGTTC AGCAAGAGTT CTATGGGGAT
    GGTACATCGT GCCCTCACTT GGTTGAAGAC ACAATAGATT TTGAAGCATT CAAATCCTTT
    TTGAAAACAT ACCTGGAAGT AGATGACATT CCCAACAATC TCTGTGAGCG TCTCTTCATG
    TCTTTTCAAG CAACCCCAAT TGAGAGCAAA AAAAGTGGGG AGGATGAGAC AGAAGGCATA
    AAAGGTAGTG GCCGTATCAG TCTAAATGAT GTGTCCTGTT ATTTCTCTTT GCTGGAAAGT
    GGCACTGCTG AGGATAAACT GGCATTCACC TTTAAATTGT ATGACAAAGA CAGCAATGGT
    CTTCTGGACA GCTCTGAGGT GGACAGGATT ATAACACAAA TGATGCGTGT AGCGGAATAT
    CTGGACTGGG ACATCACGGA ACTCAAACCT ATGTTAGAGG AGATGATGAG ACAAGTGGAC
    TATGATGGAA ATGGAATAGT CTCTTATGAA GAATGGGTTA AAGGAGGACT CACAACAGTG
    CCTCTGCTGG TGCTTCTAGG AATGGAAGTT AATATAAAAG ACGATGGGCA GCACCTGTGG
    CGCCTGAAGC ATTTCAACCG CCCTGTGTAT TGCAATGTGT GTGAAGCTCT TCTTTTTGGG
    GTCAGGAAGC AGGGCCTGTG TTGCACTTTC TGTAAGTTTA CCGTTCATGA ACGCTGTGCC
    CGCAAAGCGC CTAATTCCTG CATAAGCACG TATGCCAAAT CACGCAAGGA TTTCAGCGAG
    CAGGCCCATG TTTGGATGAG AGGTGGATGT GAGGCCAGCA AGTGTGACAA ATGCCAAAAA
    AAAATAAAAA GTTTCCAGAG CCTGACTGGG CAGCGCTGTG TGTGGTGTCA TACAAAGGTT
    CATGATGAAT GTGCCTCATC CCCACTGTGT GATGGTGGAA TCTTAAAGGA CCACATATTA
    CCTCCTGCTT GCATATATCC AGTAATCCTG GAACGACAGA ATAGCCAAAA AAATCTTCTA
    GCTTCCTCAG AAGAAGCATC ACAGACGTTT AGTACCCCAG AAGGACAAGT CCTGCGGATT
    GCCCCATTAC CAGGTACACA CCCTCTACTT GTCTTTGTAA ACCCAAAGAG TGGAGGGAAG
    CAGGGGGAAA GAGTGCTGAG GAAATTCCAG TATCTGCTAA ATCCCAGACA AGTCCATAAC
    TTGATAAAGG GAGGCCCTTC CCCAGGGCTG AATTTTTTCC GGGATGTCCC AGAATATCGT
    ATCTTAGTAT GCGGCGGTGA TGGAACAGTC GGTTGGATCC TGGATGCTAT TGACAAGGCA
    AACCTTCCAT ATAGACCACC TGTAGCTGTG CTGCCCCTCG GTACAGGAAA TGACCTTGCG
    CGATGTCTCA GCTGGGGAGG TGGTTATGAT GGAGAAAATC TAATGAAGTT CTTGAAGGAC
    ATTGAAAAGA GTGTACCCAT TAAAATGGAC AGATGGAACA TTGAGGTTAT ACCTGAAAAC
    CCAGATGAGA AGGGAGACCC AGTTCCTTAC GAGATTATAA ACAACTACTT TTCCATTGGG
    GTGGATGCAT CTATAGCTCA CAGATTCCAT AATATGAGGG AAAAATATCC AGAGAAATTC
    AATAGCAGAA TGAAGAACAA ATTGTGGTAT TTTGAGTTTG CTACATCAGA GACTGTTTTC
    TCTACCTGTA AAAAACTTAA AGAGGTCATT ACAATTGAGT GTTGTGGCTC CCCGTTGAAA
    CTCAGCTCTC TGTCCCTGGA AGGCATCGCA GTTATAAATA TTCCAAGCAT GCATGGGGGT
    TCCAACCTAT GGGGAGAGAC TAAGAAACAC TGGGGAGAAC CTCGCAATGC TTCATGTCAT
    AATGCCCCTG AAGCCATTAC TGACCCTGAT GCCTTAAAGT CATGTGTACA AGACCTCAGT
    GACAAGAGGC TGGAGGTAGT AGGACTTGAG GGTGCTATTG AGATGGGGCA GATTTATACT
    GGACTGAAGA GTGCTGGCAA ACGACTTGCA ACATGTTCTG AACTAACAAT AAGGACCACA
    AAGCCCCTCC CAATGCAAAT TGATGGCGAG CCCTGGATGC AGCCACCCTG CACGATAAAA
    ATCACACATA AGAACCAGGC ACCGATGCTG CGTGCCCCTG CGCCCCGTTC TTCAAGCTTT
    TTCTCCCTGA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947183.1
    CDS360..2594
    Translation

    Target ORF information:

    RefSeq Version XM_018091694.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis diacylglycerol kinase alpha (dgka), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091694.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCGACG AGAACAAGGA GTGGGCAATC CTGAGCCCGG AGGACTTCAC CCAACTGCAG 
    AAGTACATTG ATTACAGCAC CAAGAAGGTT AAAGATGTTC AGCAAGAGTT CTATGGGGAT
    GGTACATCGT GCCCTCACTT GGTTGAAGAC ACAATAGATT TTGAAGCATT CAAATCCTTT
    TTGAAAACAT ACCTGGAAGT AGATGACATT CCCAACAATC TCTGTGAGCG TCTCTTCATG
    TCTTTTCAAG CAACCCCAAT TGAGAGCAAA AAAAGTGGGG AGGATGAGAC AGAAGGCATA
    AAAGGTAGTG GCCGTATCAG TCTAAATGAT GTGTCCTGTT ATTTCTCTTT GCTGGAAAGT
    GGCACTGCTG AGGATAAACT GGCATTCACC TTTAAATTGT ATGACAAAGA CAGCAATGGT
    CTTCTGGACA GCTCTGAGGT GGACAGGATT ATAACACAAA TGATGCGTGT AGCGGAATAT
    CTGGACTGGG ACATCACGGA ACTCAAACCT ATGTTAGAGG AGATGATGAG ACAAGTGGAC
    TATGATGGAA ATGGAATAGT CTCTTATGAA GAATGGGTTA AAGGAGGACT CACAACAGTG
    CCTCTGCTGG TGCTTCTAGG AATGGAAGTT AATATAAAAG ACGATGGGCA GCACCTGTGG
    CGCCTGAAGC ATTTCAACCG CCCTGTGTAT TGCAATGTGT GTGAAGCTCT TCTTTTTGGG
    GTCAGGAAGC AGGGCCTGTG TTGCACTTTC TGTAAGTTTA CCGTTCATGA ACGCTGTGCC
    CGCAAAGCGC CTAATTCCTG CATAAGCACG TATGCCAAAT CACGCAAGGA TTTCAGCGAG
    CAGGCCCATG TTTGGATGAG AGGTGGATGT GAGGCCAGCA AGTGTGACAA ATGCCAAAAA
    AAAATAAAAA GTTTCCAGAG CCTGACTGGG CAGCGCTGTG TGTGGTGTCA TACAAAGGTT
    CATGATGAAT GTGCCTCATC CCCACTGTGT GATGGTGGAA TCTTAAAGGA CCACATATTA
    CCTCCTGCTT GCATATATCC AGTAATCCTG GAACGACAGA ATAGCCAAAA AAATCTTCTA
    GCTTCCTCAG AAGAAGCATC ACAGACGTTT AGTACCCCAG AAGGACAAGT CCTGCGGATT
    GCCCCATTAC CAGGTACACA CCCTCTACTT GTCTTTGTAA ACCCAAAGAG TGGAGGGAAG
    CAGGGGGAAA GAGTGCTGAG GAAATTCCAG TATCTGCTAA ATCCCAGACA AGTCCATAAC
    TTGATAAAGG GAGGCCCTTC CCCAGGGCTG AATTTTTTCC GGGATGTCCC AGAATATCGT
    ATCTTAGTAT GCGGCGGTGA TGGAACAGTC GGTTGGATCC TGGATGCTAT TGACAAGGCA
    AACCTTCCAT ATAGACCACC TGTAGCTGTG CTGCCCCTCG GTACAGGAAA TGACCTTGCG
    CGATGTCTCA GCTGGGGAGG TGGTTATGAT GGAGAAAATC TAATGAAGTT CTTGAAGGAC
    ATTGAAAAGA GTGTACCCAT TAAAATGGAC AGATGGAACA TTGAGGTTAT ACCTGAAAAC
    CCAGATGAGA AGGGAGACCC AGTTCCTTAC GAGATTATAA ACAACTACTT TTCCATTGGG
    GTGGATGCAT CTATAGCTCA CAGATTCCAT AATATGAGGG AAAAATATCC AGAGAAATTC
    AATAGCAGAA TGAAGAACAA ATTGTGGTAT TTTGAGTTTG CTACATCAGA GACTGTTTTC
    TCTACCTGTA AAAAACTTAA AGAGGTCATT ACAATTGAGT GTTGTGGCTC CCCGTTGAAA
    CTCAGCTCTC TGTCCCTGGA AGGCATCGCA GTTATAAATA TTCCAAGCAT GCATGGGGGT
    TCCAACCTAT GGGGAGAGAC TAAGAAACAC TGGGGAGAAC CTCGCAATGC TTCATGTCAT
    AATGCCCCTG AAGCCATTAC TGACCCTGAT GCCTTAAAGT CATGTGTACA AGACCTCAGT
    GACAAGAGGC TGGAGGTAGT AGGACTTGAG GGTGCTATTG AGATGGGGCA GATTTATACT
    GGACTGAAGA GTGCTGGCAA ACGACTTGCA ACATGTTCTG AACTAACAAT AAGGACCACA
    AAGCCCCTCC CAATGCAAAT TGATGGCGAG CCCTGGATGC AGCCACCCTG CACGATAAAA
    ATCACACATA AGAACCAGGC ACCGATGCTG CGTGCCCCTG CGCCCCGTTC TTCAAGCTTT
    TTCTCCCTGA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa06017
    Clone ID Related Accession (Same CDS sequence) XM_012957810.2 , XM_012957812.2 , XM_012957808.2 , XM_018091693.1 , XM_012957809.2 , XM_018091697.1 , XM_012957811.2 , XM_018091692.1 , XM_012957813.2 , XM_018091694.1 , XM_012957814.2 , XM_018091698.1
    Accession Version XM_012957814.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2235bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product diacylglycerol kinase alpha isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957814.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCGACG AGAACAAGGA GTGGGCAATC CTGAGCCCGG AGGACTTCAC CCAACTGCAG 
    AAGTACATTG ATTACAGCAC CAAGAAGGTT AAAGATGTTC AGCAAGAGTT CTATGGGGAT
    GGTACATCGT GCCCTCACTT GGTTGAAGAC ACAATAGATT TTGAAGCATT CAAATCCTTT
    TTGAAAACAT ACCTGGAAGT AGATGACATT CCCAACAATC TCTGTGAGCG TCTCTTCATG
    TCTTTTCAAG CAACCCCAAT TGAGAGCAAA AAAAGTGGGG AGGATGAGAC AGAAGGCATA
    AAAGGTAGTG GCCGTATCAG TCTAAATGAT GTGTCCTGTT ATTTCTCTTT GCTGGAAAGT
    GGCACTGCTG AGGATAAACT GGCATTCACC TTTAAATTGT ATGACAAAGA CAGCAATGGT
    CTTCTGGACA GCTCTGAGGT GGACAGGATT ATAACACAAA TGATGCGTGT AGCGGAATAT
    CTGGACTGGG ACATCACGGA ACTCAAACCT ATGTTAGAGG AGATGATGAG ACAAGTGGAC
    TATGATGGAA ATGGAATAGT CTCTTATGAA GAATGGGTTA AAGGAGGACT CACAACAGTG
    CCTCTGCTGG TGCTTCTAGG AATGGAAGTT AATATAAAAG ACGATGGGCA GCACCTGTGG
    CGCCTGAAGC ATTTCAACCG CCCTGTGTAT TGCAATGTGT GTGAAGCTCT TCTTTTTGGG
    GTCAGGAAGC AGGGCCTGTG TTGCACTTTC TGTAAGTTTA CCGTTCATGA ACGCTGTGCC
    CGCAAAGCGC CTAATTCCTG CATAAGCACG TATGCCAAAT CACGCAAGGA TTTCAGCGAG
    CAGGCCCATG TTTGGATGAG AGGTGGATGT GAGGCCAGCA AGTGTGACAA ATGCCAAAAA
    AAAATAAAAA GTTTCCAGAG CCTGACTGGG CAGCGCTGTG TGTGGTGTCA TACAAAGGTT
    CATGATGAAT GTGCCTCATC CCCACTGTGT GATGGTGGAA TCTTAAAGGA CCACATATTA
    CCTCCTGCTT GCATATATCC AGTAATCCTG GAACGACAGA ATAGCCAAAA AAATCTTCTA
    GCTTCCTCAG AAGAAGCATC ACAGACGTTT AGTACCCCAG AAGGACAAGT CCTGCGGATT
    GCCCCATTAC CAGGTACACA CCCTCTACTT GTCTTTGTAA ACCCAAAGAG TGGAGGGAAG
    CAGGGGGAAA GAGTGCTGAG GAAATTCCAG TATCTGCTAA ATCCCAGACA AGTCCATAAC
    TTGATAAAGG GAGGCCCTTC CCCAGGGCTG AATTTTTTCC GGGATGTCCC AGAATATCGT
    ATCTTAGTAT GCGGCGGTGA TGGAACAGTC GGTTGGATCC TGGATGCTAT TGACAAGGCA
    AACCTTCCAT ATAGACCACC TGTAGCTGTG CTGCCCCTCG GTACAGGAAA TGACCTTGCG
    CGATGTCTCA GCTGGGGAGG TGGTTATGAT GGAGAAAATC TAATGAAGTT CTTGAAGGAC
    ATTGAAAAGA GTGTACCCAT TAAAATGGAC AGATGGAACA TTGAGGTTAT ACCTGAAAAC
    CCAGATGAGA AGGGAGACCC AGTTCCTTAC GAGATTATAA ACAACTACTT TTCCATTGGG
    GTGGATGCAT CTATAGCTCA CAGATTCCAT AATATGAGGG AAAAATATCC AGAGAAATTC
    AATAGCAGAA TGAAGAACAA ATTGTGGTAT TTTGAGTTTG CTACATCAGA GACTGTTTTC
    TCTACCTGTA AAAAACTTAA AGAGGTCATT ACAATTGAGT GTTGTGGCTC CCCGTTGAAA
    CTCAGCTCTC TGTCCCTGGA AGGCATCGCA GTTATAAATA TTCCAAGCAT GCATGGGGGT
    TCCAACCTAT GGGGAGAGAC TAAGAAACAC TGGGGAGAAC CTCGCAATGC TTCATGTCAT
    AATGCCCCTG AAGCCATTAC TGACCCTGAT GCCTTAAAGT CATGTGTACA AGACCTCAGT
    GACAAGAGGC TGGAGGTAGT AGGACTTGAG GGTGCTATTG AGATGGGGCA GATTTATACT
    GGACTGAAGA GTGCTGGCAA ACGACTTGCA ACATGTTCTG AACTAACAAT AAGGACCACA
    AAGCCCCTCC CAATGCAAAT TGATGGCGAG CCCTGGATGC AGCCACCCTG CACGATAAAA
    ATCACACATA AGAACCAGGC ACCGATGCTG CGTGCCCCTG CGCCCCGTTC TTCAAGCTTT
    TTCTCCCTGA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813268.1
    CDS334..2568
    Translation

    Target ORF information:

    RefSeq Version XM_012957814.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis diacylglycerol kinase alpha (dgka), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957814.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCGACG AGAACAAGGA GTGGGCAATC CTGAGCCCGG AGGACTTCAC CCAACTGCAG 
    AAGTACATTG ATTACAGCAC CAAGAAGGTT AAAGATGTTC AGCAAGAGTT CTATGGGGAT
    GGTACATCGT GCCCTCACTT GGTTGAAGAC ACAATAGATT TTGAAGCATT CAAATCCTTT
    TTGAAAACAT ACCTGGAAGT AGATGACATT CCCAACAATC TCTGTGAGCG TCTCTTCATG
    TCTTTTCAAG CAACCCCAAT TGAGAGCAAA AAAAGTGGGG AGGATGAGAC AGAAGGCATA
    AAAGGTAGTG GCCGTATCAG TCTAAATGAT GTGTCCTGTT ATTTCTCTTT GCTGGAAAGT
    GGCACTGCTG AGGATAAACT GGCATTCACC TTTAAATTGT ATGACAAAGA CAGCAATGGT
    CTTCTGGACA GCTCTGAGGT GGACAGGATT ATAACACAAA TGATGCGTGT AGCGGAATAT
    CTGGACTGGG ACATCACGGA ACTCAAACCT ATGTTAGAGG AGATGATGAG ACAAGTGGAC
    TATGATGGAA ATGGAATAGT CTCTTATGAA GAATGGGTTA AAGGAGGACT CACAACAGTG
    CCTCTGCTGG TGCTTCTAGG AATGGAAGTT AATATAAAAG ACGATGGGCA GCACCTGTGG
    CGCCTGAAGC ATTTCAACCG CCCTGTGTAT TGCAATGTGT GTGAAGCTCT TCTTTTTGGG
    GTCAGGAAGC AGGGCCTGTG TTGCACTTTC TGTAAGTTTA CCGTTCATGA ACGCTGTGCC
    CGCAAAGCGC CTAATTCCTG CATAAGCACG TATGCCAAAT CACGCAAGGA TTTCAGCGAG
    CAGGCCCATG TTTGGATGAG AGGTGGATGT GAGGCCAGCA AGTGTGACAA ATGCCAAAAA
    AAAATAAAAA GTTTCCAGAG CCTGACTGGG CAGCGCTGTG TGTGGTGTCA TACAAAGGTT
    CATGATGAAT GTGCCTCATC CCCACTGTGT GATGGTGGAA TCTTAAAGGA CCACATATTA
    CCTCCTGCTT GCATATATCC AGTAATCCTG GAACGACAGA ATAGCCAAAA AAATCTTCTA
    GCTTCCTCAG AAGAAGCATC ACAGACGTTT AGTACCCCAG AAGGACAAGT CCTGCGGATT
    GCCCCATTAC CAGGTACACA CCCTCTACTT GTCTTTGTAA ACCCAAAGAG TGGAGGGAAG
    CAGGGGGAAA GAGTGCTGAG GAAATTCCAG TATCTGCTAA ATCCCAGACA AGTCCATAAC
    TTGATAAAGG GAGGCCCTTC CCCAGGGCTG AATTTTTTCC GGGATGTCCC AGAATATCGT
    ATCTTAGTAT GCGGCGGTGA TGGAACAGTC GGTTGGATCC TGGATGCTAT TGACAAGGCA
    AACCTTCCAT ATAGACCACC TGTAGCTGTG CTGCCCCTCG GTACAGGAAA TGACCTTGCG
    CGATGTCTCA GCTGGGGAGG TGGTTATGAT GGAGAAAATC TAATGAAGTT CTTGAAGGAC
    ATTGAAAAGA GTGTACCCAT TAAAATGGAC AGATGGAACA TTGAGGTTAT ACCTGAAAAC
    CCAGATGAGA AGGGAGACCC AGTTCCTTAC GAGATTATAA ACAACTACTT TTCCATTGGG
    GTGGATGCAT CTATAGCTCA CAGATTCCAT AATATGAGGG AAAAATATCC AGAGAAATTC
    AATAGCAGAA TGAAGAACAA ATTGTGGTAT TTTGAGTTTG CTACATCAGA GACTGTTTTC
    TCTACCTGTA AAAAACTTAA AGAGGTCATT ACAATTGAGT GTTGTGGCTC CCCGTTGAAA
    CTCAGCTCTC TGTCCCTGGA AGGCATCGCA GTTATAAATA TTCCAAGCAT GCATGGGGGT
    TCCAACCTAT GGGGAGAGAC TAAGAAACAC TGGGGAGAAC CTCGCAATGC TTCATGTCAT
    AATGCCCCTG AAGCCATTAC TGACCCTGAT GCCTTAAAGT CATGTGTACA AGACCTCAGT
    GACAAGAGGC TGGAGGTAGT AGGACTTGAG GGTGCTATTG AGATGGGGCA GATTTATACT
    GGACTGAAGA GTGCTGGCAA ACGACTTGCA ACATGTTCTG AACTAACAAT AAGGACCACA
    AAGCCCCTCC CAATGCAAAT TGATGGCGAG CCCTGGATGC AGCCACCCTG CACGATAAAA
    ATCACACATA AGAACCAGGC ACCGATGCTG CGTGCCCCTG CGCCCCGTTC TTCAAGCTTT
    TTCTCCCTGA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa06017
    Clone ID Related Accession (Same CDS sequence) XM_012957810.2 , XM_012957812.2 , XM_012957808.2 , XM_018091693.1 , XM_012957809.2 , XM_018091697.1 , XM_012957811.2 , XM_018091692.1 , XM_012957813.2 , XM_018091694.1 , XM_012957814.2 , XM_018091698.1
    Accession Version XM_018091698.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2235bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product diacylglycerol kinase alpha isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCGACG AGAACAAGGA GTGGGCAATC CTGAGCCCGG AGGACTTCAC CCAACTGCAG 
    AAGTACATTG ATTACAGCAC CAAGAAGGTT AAAGATGTTC AGCAAGAGTT CTATGGGGAT
    GGTACATCGT GCCCTCACTT GGTTGAAGAC ACAATAGATT TTGAAGCATT CAAATCCTTT
    TTGAAAACAT ACCTGGAAGT AGATGACATT CCCAACAATC TCTGTGAGCG TCTCTTCATG
    TCTTTTCAAG CAACCCCAAT TGAGAGCAAA AAAAGTGGGG AGGATGAGAC AGAAGGCATA
    AAAGGTAGTG GCCGTATCAG TCTAAATGAT GTGTCCTGTT ATTTCTCTTT GCTGGAAAGT
    GGCACTGCTG AGGATAAACT GGCATTCACC TTTAAATTGT ATGACAAAGA CAGCAATGGT
    CTTCTGGACA GCTCTGAGGT GGACAGGATT ATAACACAAA TGATGCGTGT AGCGGAATAT
    CTGGACTGGG ACATCACGGA ACTCAAACCT ATGTTAGAGG AGATGATGAG ACAAGTGGAC
    TATGATGGAA ATGGAATAGT CTCTTATGAA GAATGGGTTA AAGGAGGACT CACAACAGTG
    CCTCTGCTGG TGCTTCTAGG AATGGAAGTT AATATAAAAG ACGATGGGCA GCACCTGTGG
    CGCCTGAAGC ATTTCAACCG CCCTGTGTAT TGCAATGTGT GTGAAGCTCT TCTTTTTGGG
    GTCAGGAAGC AGGGCCTGTG TTGCACTTTC TGTAAGTTTA CCGTTCATGA ACGCTGTGCC
    CGCAAAGCGC CTAATTCCTG CATAAGCACG TATGCCAAAT CACGCAAGGA TTTCAGCGAG
    CAGGCCCATG TTTGGATGAG AGGTGGATGT GAGGCCAGCA AGTGTGACAA ATGCCAAAAA
    AAAATAAAAA GTTTCCAGAG CCTGACTGGG CAGCGCTGTG TGTGGTGTCA TACAAAGGTT
    CATGATGAAT GTGCCTCATC CCCACTGTGT GATGGTGGAA TCTTAAAGGA CCACATATTA
    CCTCCTGCTT GCATATATCC AGTAATCCTG GAACGACAGA ATAGCCAAAA AAATCTTCTA
    GCTTCCTCAG AAGAAGCATC ACAGACGTTT AGTACCCCAG AAGGACAAGT CCTGCGGATT
    GCCCCATTAC CAGGTACACA CCCTCTACTT GTCTTTGTAA ACCCAAAGAG TGGAGGGAAG
    CAGGGGGAAA GAGTGCTGAG GAAATTCCAG TATCTGCTAA ATCCCAGACA AGTCCATAAC
    TTGATAAAGG GAGGCCCTTC CCCAGGGCTG AATTTTTTCC GGGATGTCCC AGAATATCGT
    ATCTTAGTAT GCGGCGGTGA TGGAACAGTC GGTTGGATCC TGGATGCTAT TGACAAGGCA
    AACCTTCCAT ATAGACCACC TGTAGCTGTG CTGCCCCTCG GTACAGGAAA TGACCTTGCG
    CGATGTCTCA GCTGGGGAGG TGGTTATGAT GGAGAAAATC TAATGAAGTT CTTGAAGGAC
    ATTGAAAAGA GTGTACCCAT TAAAATGGAC AGATGGAACA TTGAGGTTAT ACCTGAAAAC
    CCAGATGAGA AGGGAGACCC AGTTCCTTAC GAGATTATAA ACAACTACTT TTCCATTGGG
    GTGGATGCAT CTATAGCTCA CAGATTCCAT AATATGAGGG AAAAATATCC AGAGAAATTC
    AATAGCAGAA TGAAGAACAA ATTGTGGTAT TTTGAGTTTG CTACATCAGA GACTGTTTTC
    TCTACCTGTA AAAAACTTAA AGAGGTCATT ACAATTGAGT GTTGTGGCTC CCCGTTGAAA
    CTCAGCTCTC TGTCCCTGGA AGGCATCGCA GTTATAAATA TTCCAAGCAT GCATGGGGGT
    TCCAACCTAT GGGGAGAGAC TAAGAAACAC TGGGGAGAAC CTCGCAATGC TTCATGTCAT
    AATGCCCCTG AAGCCATTAC TGACCCTGAT GCCTTAAAGT CATGTGTACA AGACCTCAGT
    GACAAGAGGC TGGAGGTAGT AGGACTTGAG GGTGCTATTG AGATGGGGCA GATTTATACT
    GGACTGAAGA GTGCTGGCAA ACGACTTGCA ACATGTTCTG AACTAACAAT AAGGACCACA
    AAGCCCCTCC CAATGCAAAT TGATGGCGAG CCCTGGATGC AGCCACCCTG CACGATAAAA
    ATCACACATA AGAACCAGGC ACCGATGCTG CGTGCCCCTG CGCCCCGTTC TTCAAGCTTT
    TTCTCCCTGA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947187.1
    CDS163..2397
    Translation

    Target ORF information:

    RefSeq Version XM_018091698.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis diacylglycerol kinase alpha (dgka), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091698.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCGACG AGAACAAGGA GTGGGCAATC CTGAGCCCGG AGGACTTCAC CCAACTGCAG 
    AAGTACATTG ATTACAGCAC CAAGAAGGTT AAAGATGTTC AGCAAGAGTT CTATGGGGAT
    GGTACATCGT GCCCTCACTT GGTTGAAGAC ACAATAGATT TTGAAGCATT CAAATCCTTT
    TTGAAAACAT ACCTGGAAGT AGATGACATT CCCAACAATC TCTGTGAGCG TCTCTTCATG
    TCTTTTCAAG CAACCCCAAT TGAGAGCAAA AAAAGTGGGG AGGATGAGAC AGAAGGCATA
    AAAGGTAGTG GCCGTATCAG TCTAAATGAT GTGTCCTGTT ATTTCTCTTT GCTGGAAAGT
    GGCACTGCTG AGGATAAACT GGCATTCACC TTTAAATTGT ATGACAAAGA CAGCAATGGT
    CTTCTGGACA GCTCTGAGGT GGACAGGATT ATAACACAAA TGATGCGTGT AGCGGAATAT
    CTGGACTGGG ACATCACGGA ACTCAAACCT ATGTTAGAGG AGATGATGAG ACAAGTGGAC
    TATGATGGAA ATGGAATAGT CTCTTATGAA GAATGGGTTA AAGGAGGACT CACAACAGTG
    CCTCTGCTGG TGCTTCTAGG AATGGAAGTT AATATAAAAG ACGATGGGCA GCACCTGTGG
    CGCCTGAAGC ATTTCAACCG CCCTGTGTAT TGCAATGTGT GTGAAGCTCT TCTTTTTGGG
    GTCAGGAAGC AGGGCCTGTG TTGCACTTTC TGTAAGTTTA CCGTTCATGA ACGCTGTGCC
    CGCAAAGCGC CTAATTCCTG CATAAGCACG TATGCCAAAT CACGCAAGGA TTTCAGCGAG
    CAGGCCCATG TTTGGATGAG AGGTGGATGT GAGGCCAGCA AGTGTGACAA ATGCCAAAAA
    AAAATAAAAA GTTTCCAGAG CCTGACTGGG CAGCGCTGTG TGTGGTGTCA TACAAAGGTT
    CATGATGAAT GTGCCTCATC CCCACTGTGT GATGGTGGAA TCTTAAAGGA CCACATATTA
    CCTCCTGCTT GCATATATCC AGTAATCCTG GAACGACAGA ATAGCCAAAA AAATCTTCTA
    GCTTCCTCAG AAGAAGCATC ACAGACGTTT AGTACCCCAG AAGGACAAGT CCTGCGGATT
    GCCCCATTAC CAGGTACACA CCCTCTACTT GTCTTTGTAA ACCCAAAGAG TGGAGGGAAG
    CAGGGGGAAA GAGTGCTGAG GAAATTCCAG TATCTGCTAA ATCCCAGACA AGTCCATAAC
    TTGATAAAGG GAGGCCCTTC CCCAGGGCTG AATTTTTTCC GGGATGTCCC AGAATATCGT
    ATCTTAGTAT GCGGCGGTGA TGGAACAGTC GGTTGGATCC TGGATGCTAT TGACAAGGCA
    AACCTTCCAT ATAGACCACC TGTAGCTGTG CTGCCCCTCG GTACAGGAAA TGACCTTGCG
    CGATGTCTCA GCTGGGGAGG TGGTTATGAT GGAGAAAATC TAATGAAGTT CTTGAAGGAC
    ATTGAAAAGA GTGTACCCAT TAAAATGGAC AGATGGAACA TTGAGGTTAT ACCTGAAAAC
    CCAGATGAGA AGGGAGACCC AGTTCCTTAC GAGATTATAA ACAACTACTT TTCCATTGGG
    GTGGATGCAT CTATAGCTCA CAGATTCCAT AATATGAGGG AAAAATATCC AGAGAAATTC
    AATAGCAGAA TGAAGAACAA ATTGTGGTAT TTTGAGTTTG CTACATCAGA GACTGTTTTC
    TCTACCTGTA AAAAACTTAA AGAGGTCATT ACAATTGAGT GTTGTGGCTC CCCGTTGAAA
    CTCAGCTCTC TGTCCCTGGA AGGCATCGCA GTTATAAATA TTCCAAGCAT GCATGGGGGT
    TCCAACCTAT GGGGAGAGAC TAAGAAACAC TGGGGAGAAC CTCGCAATGC TTCATGTCAT
    AATGCCCCTG AAGCCATTAC TGACCCTGAT GCCTTAAAGT CATGTGTACA AGACCTCAGT
    GACAAGAGGC TGGAGGTAGT AGGACTTGAG GGTGCTATTG AGATGGGGCA GATTTATACT
    GGACTGAAGA GTGCTGGCAA ACGACTTGCA ACATGTTCTG AACTAACAAT AAGGACCACA
    AAGCCCCTCC CAATGCAAAT TGATGGCGAG CCCTGGATGC AGCCACCCTG CACGATAAAA
    ATCACACATA AGAACCAGGC ACCGATGCTG CGTGCCCCTG CGCCCCGTTC TTCAAGCTTT
    TTCTCCCTGA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa42842
    Clone ID Related Accession (Same CDS sequence) XM_018091695.1
    Accession Version XM_018091695.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1998bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product diacylglycerol kinase alpha isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGTCTTTTC AAGCAACCCC AATTGAGAGC AAAAAAAGTG GGGAGGATGA GACAGAAGGC 
    ATAAAAGGTA GTGGCCGTAT CAGTCTAAAT GATGTGTCCT GTTATTTCTC TTTGCTGGAA
    AGTGGCACTG CTGAGGATAA ACTGGCATTC ACCTTTAAAT TGTATGACAA AGACAGCAAT
    GGTCTTCTGG ACAGCTCTGA GGTGGACAGG ATTATAACAC AAATGATGCG TGTAGCGGAA
    TATCTGGACT GGGACATCAC GGAACTCAAA CCTATGTTAG AGGAGATGAT GAGACAAGTG
    GACTATGATG GAAATGGAAT AGTCTCTTAT GAAGAATGGG TTAAAGGAGG ACTCACAACA
    GTGCCTCTGC TGGTGCTTCT AGGAATGGAA GTTAATATAA AAGACGATGG GCAGCACCTG
    TGGCGCCTGA AGCATTTCAA CCGCCCTGTG TATTGCAATG TGTGTGAAGC TCTTCTTTTT
    GGGGTCAGGA AGCAGGGCCT GTGTTGCACT TTCTGTAAGT TTACCGTTCA TGAACGCTGT
    GCCCGCAAAG CGCCTAATTC CTGCATAAGC ACGTATGCCA AATCACGCAA GGATTTCAGC
    GAGCAGGCCC ATGTTTGGAT GAGAGGTGGA TGTGAGGCCA GCAAGTGTGA CAAATGCCAA
    AAAAAAATAA AAAGTTTCCA GAGCCTGACT GGGCAGCGCT GTGTGTGGTG TCATACAAAG
    GTTCATGATG AATGTGCCTC ATCCCCACTG TGTGATGGTG GAATCTTAAA GGACCACATA
    TTACCTCCTG CTTGCATATA TCCAGTAATC CTGGAACGAC AGAATAGCCA AAAAAATCTT
    CTAGCTTCCT CAGAAGAAGC ATCACAGACG TTTAGTACCC CAGAAGGACA AGTCCTGCGG
    ATTGCCCCAT TACCAGGTAC ACACCCTCTA CTTGTCTTTG TAAACCCAAA GAGTGGAGGG
    AAGCAGGGGG AAAGAGTGCT GAGGAAATTC CAGTATCTGC TAAATCCCAG ACAAGTCCAT
    AACTTGATAA AGGGAGGCCC TTCCCCAGGG CTGAATTTTT TCCGGGATGT CCCAGAATAT
    CGTATCTTAG TATGCGGCGG TGATGGAACA GTCGGTTGGA TCCTGGATGC TATTGACAAG
    GCAAACCTTC CATATAGACC ACCTGTAGCT GTGCTGCCCC TCGGTACAGG AAATGACCTT
    GCGCGATGTC TCAGCTGGGG AGGTGGTTAT GATGGAGAAA ATCTAATGAA GTTCTTGAAG
    GACATTGAAA AGAGTGTACC CATTAAAATG GACAGATGGA ACATTGAGGT TATACCTGAA
    AACCCAGATG AGAAGGGAGA CCCAGTTCCT TACGAGATTA TAAACAACTA CTTTTCCATT
    GGGGTGGATG CATCTATAGC TCACAGATTC CATAATATGA GGGAAAAATA TCCAGAGAAA
    TTCAATAGCA GAATGAAGAA CAAATTGTGG TATTTTGAGT TTGCTACATC AGAGACTGTT
    TTCTCTACCT GTAAAAAACT TAAAGAGGTC ATTACAATTG AGTGTTGTGG CTCCCCGTTG
    AAACTCAGCT CTCTGTCCCT GGAAGGCATC GCAGTTATAA ATATTCCAAG CATGCATGGG
    GGTTCCAACC TATGGGGAGA GACTAAGAAA CACTGGGGAG AACCTCGCAA TGCTTCATGT
    CATAATGCCC CTGAAGCCAT TACTGACCCT GATGCCTTAA AGTCATGTGT ACAAGACCTC
    AGTGACAAGA GGCTGGAGGT AGTAGGACTT GAGGGTGCTA TTGAGATGGG GCAGATTTAT
    ACTGGACTGA AGAGTGCTGG CAAACGACTT GCAACATGTT CTGAACTAAC AATAAGGACC
    ACAAAGCCCC TCCCAATGCA AATTGATGGC GAGCCCTGGA TGCAGCCACC CTGCACGATA
    AAAATCACAC ATAAGAACCA GGCACCGATG CTGCGTGCCC CTGCGCCCCG TTCTTCAAGC
    TTTTTCTCCC TGAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947184.1
    CDS175..2172
    Translation

    Target ORF information:

    RefSeq Version XM_018091695.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis diacylglycerol kinase alpha (dgka), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091695.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGTCTTTTC AAGCAACCCC AATTGAGAGC AAAAAAAGTG GGGAGGATGA GACAGAAGGC 
    ATAAAAGGTA GTGGCCGTAT CAGTCTAAAT GATGTGTCCT GTTATTTCTC TTTGCTGGAA
    AGTGGCACTG CTGAGGATAA ACTGGCATTC ACCTTTAAAT TGTATGACAA AGACAGCAAT
    GGTCTTCTGG ACAGCTCTGA GGTGGACAGG ATTATAACAC AAATGATGCG TGTAGCGGAA
    TATCTGGACT GGGACATCAC GGAACTCAAA CCTATGTTAG AGGAGATGAT GAGACAAGTG
    GACTATGATG GAAATGGAAT AGTCTCTTAT GAAGAATGGG TTAAAGGAGG ACTCACAACA
    GTGCCTCTGC TGGTGCTTCT AGGAATGGAA GTTAATATAA AAGACGATGG GCAGCACCTG
    TGGCGCCTGA AGCATTTCAA CCGCCCTGTG TATTGCAATG TGTGTGAAGC TCTTCTTTTT
    GGGGTCAGGA AGCAGGGCCT GTGTTGCACT TTCTGTAAGT TTACCGTTCA TGAACGCTGT
    GCCCGCAAAG CGCCTAATTC CTGCATAAGC ACGTATGCCA AATCACGCAA GGATTTCAGC
    GAGCAGGCCC ATGTTTGGAT GAGAGGTGGA TGTGAGGCCA GCAAGTGTGA CAAATGCCAA
    AAAAAAATAA AAAGTTTCCA GAGCCTGACT GGGCAGCGCT GTGTGTGGTG TCATACAAAG
    GTTCATGATG AATGTGCCTC ATCCCCACTG TGTGATGGTG GAATCTTAAA GGACCACATA
    TTACCTCCTG CTTGCATATA TCCAGTAATC CTGGAACGAC AGAATAGCCA AAAAAATCTT
    CTAGCTTCCT CAGAAGAAGC ATCACAGACG TTTAGTACCC CAGAAGGACA AGTCCTGCGG
    ATTGCCCCAT TACCAGGTAC ACACCCTCTA CTTGTCTTTG TAAACCCAAA GAGTGGAGGG
    AAGCAGGGGG AAAGAGTGCT GAGGAAATTC CAGTATCTGC TAAATCCCAG ACAAGTCCAT
    AACTTGATAA AGGGAGGCCC TTCCCCAGGG CTGAATTTTT TCCGGGATGT CCCAGAATAT
    CGTATCTTAG TATGCGGCGG TGATGGAACA GTCGGTTGGA TCCTGGATGC TATTGACAAG
    GCAAACCTTC CATATAGACC ACCTGTAGCT GTGCTGCCCC TCGGTACAGG AAATGACCTT
    GCGCGATGTC TCAGCTGGGG AGGTGGTTAT GATGGAGAAA ATCTAATGAA GTTCTTGAAG
    GACATTGAAA AGAGTGTACC CATTAAAATG GACAGATGGA ACATTGAGGT TATACCTGAA
    AACCCAGATG AGAAGGGAGA CCCAGTTCCT TACGAGATTA TAAACAACTA CTTTTCCATT
    GGGGTGGATG CATCTATAGC TCACAGATTC CATAATATGA GGGAAAAATA TCCAGAGAAA
    TTCAATAGCA GAATGAAGAA CAAATTGTGG TATTTTGAGT TTGCTACATC AGAGACTGTT
    TTCTCTACCT GTAAAAAACT TAAAGAGGTC ATTACAATTG AGTGTTGTGG CTCCCCGTTG
    AAACTCAGCT CTCTGTCCCT GGAAGGCATC GCAGTTATAA ATATTCCAAG CATGCATGGG
    GGTTCCAACC TATGGGGAGA GACTAAGAAA CACTGGGGAG AACCTCGCAA TGCTTCATGT
    CATAATGCCC CTGAAGCCAT TACTGACCCT GATGCCTTAA AGTCATGTGT ACAAGACCTC
    AGTGACAAGA GGCTGGAGGT AGTAGGACTT GAGGGTGCTA TTGAGATGGG GCAGATTTAT
    ACTGGACTGA AGAGTGCTGG CAAACGACTT GCAACATGTT CTGAACTAAC AATAAGGACC
    ACAAAGCCCC TCCCAATGCA AATTGATGGC GAGCCCTGGA TGCAGCCACC CTGCACGATA
    AAAATCACAC ATAAGAACCA GGCACCGATG CTGCGTGCCC CTGCGCCCCG TTCTTCAAGC
    TTTTTCTCCC TGAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa42843
    Clone ID Related Accession (Same CDS sequence) XM_018091696.1
    Accession Version XM_018091696.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1860bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product diacylglycerol kinase alpha isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGATGTGTC CTGTTATTTC TCTTTGCTGG AAAGTGGCAC TGCTGAGGAT AAACTGGCAT 
    GAGGTGGACA GGATTATAAC ACAAATGATG CGTGTAGCGG AATATCTGGA CTGGGACATC
    ACGGAACTCA AACCTATGTT AGAGGAGATG ATGAGACAAG TGGACTATGA TGGAAATGGA
    ATAGTCTCTT ATGAAGAATG GGTTAAAGGA GGACTCACAA CAGTGCCTCT GCTGGTGCTT
    CTAGGAATGG AAGTTAATAT AAAAGACGAT GGGCAGCACC TGTGGCGCCT GAAGCATTTC
    AACCGCCCTG TGTATTGCAA TGTGTGTGAA GCTCTTCTTT TTGGGGTCAG GAAGCAGGGC
    CTGTGTTGCA CTTTCTGTAA GTTTACCGTT CATGAACGCT GTGCCCGCAA AGCGCCTAAT
    TCCTGCATAA GCACGTATGC CAAATCACGC AAGGATTTCA GCGAGCAGGC CCATGTTTGG
    ATGAGAGGTG GATGTGAGGC CAGCAAGTGT GACAAATGCC AAAAAAAAAT AAAAAGTTTC
    CAGAGCCTGA CTGGGCAGCG CTGTGTGTGG TGTCATACAA AGGTTCATGA TGAATGTGCC
    TCATCCCCAC TGTGTGATGG TGGAATCTTA AAGGACCACA TATTACCTCC TGCTTGCATA
    TATCCAGTAA TCCTGGAACG ACAGAATAGC CAAAAAAATC TTCTAGCTTC CTCAGAAGAA
    GCATCACAGA CGTTTAGTAC CCCAGAAGGA CAAGTCCTGC GGATTGCCCC ATTACCAGGT
    ACACACCCTC TACTTGTCTT TGTAAACCCA AAGAGTGGAG GGAAGCAGGG GGAAAGAGTG
    CTGAGGAAAT TCCAGTATCT GCTAAATCCC AGACAAGTCC ATAACTTGAT AAAGGGAGGC
    CCTTCCCCAG GGCTGAATTT TTTCCGGGAT GTCCCAGAAT ATCGTATCTT AGTATGCGGC
    GGTGATGGAA CAGTCGGTTG GATCCTGGAT GCTATTGACA AGGCAAACCT TCCATATAGA
    CCACCTGTAG CTGTGCTGCC CCTCGGTACA GGAAATGACC TTGCGCGATG TCTCAGCTGG
    GGAGGTGGTT ATGATGGAGA AAATCTAATG AAGTTCTTGA AGGACATTGA AAAGAGTGTA
    CCCATTAAAA TGGACAGATG GAACATTGAG GTTATACCTG AAAACCCAGA TGAGAAGGGA
    GACCCAGTTC CTTACGAGAT TATAAACAAC TACTTTTCCA TTGGGGTGGA TGCATCTATA
    GCTCACAGAT TCCATAATAT GAGGGAAAAA TATCCAGAGA AATTCAATAG CAGAATGAAG
    AACAAATTGT GGTATTTTGA GTTTGCTACA TCAGAGACTG TTTTCTCTAC CTGTAAAAAA
    CTTAAAGAGG TCATTACAAT TGAGTGTTGT GGCTCCCCGT TGAAACTCAG CTCTCTGTCC
    CTGGAAGGCA TCGCAGTTAT AAATATTCCA AGCATGCATG GGGGTTCCAA CCTATGGGGA
    GAGACTAAGA AACACTGGGG AGAACCTCGC AATGCTTCAT GTCATAATGC CCCTGAAGCC
    ATTACTGACC CTGATGCCTT AAAGTCATGT GTACAAGACC TCAGTGACAA GAGGCTGGAG
    GTAGTAGGAC TTGAGGGTGC TATTGAGATG GGGCAGATTT ATACTGGACT GAAGAGTGCT
    GGCAAACGAC TTGCAACATG TTCTGAACTA ACAATAAGGA CCACAAAGCC CCTCCCAATG
    CAAATTGATG GCGAGCCCTG GATGCAGCCA CCCTGCACGA TAAAAATCAC ACATAAGAAC
    CAGGCACCGA TGCTGCGTGC CCCTGCGCCC CGTTCTTCAA GCTTTTTCTC CCTGAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947185.1
    CDS263..2122
    Translation

    Target ORF information:

    RefSeq Version XM_018091696.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis diacylglycerol kinase alpha (dgka), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091696.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGATGTGTC CTGTTATTTC TCTTTGCTGG AAAGTGGCAC TGCTGAGGAT AAACTGGCAT 
    GAGGTGGACA GGATTATAAC ACAAATGATG CGTGTAGCGG AATATCTGGA CTGGGACATC
    ACGGAACTCA AACCTATGTT AGAGGAGATG ATGAGACAAG TGGACTATGA TGGAAATGGA
    ATAGTCTCTT ATGAAGAATG GGTTAAAGGA GGACTCACAA CAGTGCCTCT GCTGGTGCTT
    CTAGGAATGG AAGTTAATAT AAAAGACGAT GGGCAGCACC TGTGGCGCCT GAAGCATTTC
    AACCGCCCTG TGTATTGCAA TGTGTGTGAA GCTCTTCTTT TTGGGGTCAG GAAGCAGGGC
    CTGTGTTGCA CTTTCTGTAA GTTTACCGTT CATGAACGCT GTGCCCGCAA AGCGCCTAAT
    TCCTGCATAA GCACGTATGC CAAATCACGC AAGGATTTCA GCGAGCAGGC CCATGTTTGG
    ATGAGAGGTG GATGTGAGGC CAGCAAGTGT GACAAATGCC AAAAAAAAAT AAAAAGTTTC
    CAGAGCCTGA CTGGGCAGCG CTGTGTGTGG TGTCATACAA AGGTTCATGA TGAATGTGCC
    TCATCCCCAC TGTGTGATGG TGGAATCTTA AAGGACCACA TATTACCTCC TGCTTGCATA
    TATCCAGTAA TCCTGGAACG ACAGAATAGC CAAAAAAATC TTCTAGCTTC CTCAGAAGAA
    GCATCACAGA CGTTTAGTAC CCCAGAAGGA CAAGTCCTGC GGATTGCCCC ATTACCAGGT
    ACACACCCTC TACTTGTCTT TGTAAACCCA AAGAGTGGAG GGAAGCAGGG GGAAAGAGTG
    CTGAGGAAAT TCCAGTATCT GCTAAATCCC AGACAAGTCC ATAACTTGAT AAAGGGAGGC
    CCTTCCCCAG GGCTGAATTT TTTCCGGGAT GTCCCAGAAT ATCGTATCTT AGTATGCGGC
    GGTGATGGAA CAGTCGGTTG GATCCTGGAT GCTATTGACA AGGCAAACCT TCCATATAGA
    CCACCTGTAG CTGTGCTGCC CCTCGGTACA GGAAATGACC TTGCGCGATG TCTCAGCTGG
    GGAGGTGGTT ATGATGGAGA AAATCTAATG AAGTTCTTGA AGGACATTGA AAAGAGTGTA
    CCCATTAAAA TGGACAGATG GAACATTGAG GTTATACCTG AAAACCCAGA TGAGAAGGGA
    GACCCAGTTC CTTACGAGAT TATAAACAAC TACTTTTCCA TTGGGGTGGA TGCATCTATA
    GCTCACAGAT TCCATAATAT GAGGGAAAAA TATCCAGAGA AATTCAATAG CAGAATGAAG
    AACAAATTGT GGTATTTTGA GTTTGCTACA TCAGAGACTG TTTTCTCTAC CTGTAAAAAA
    CTTAAAGAGG TCATTACAAT TGAGTGTTGT GGCTCCCCGT TGAAACTCAG CTCTCTGTCC
    CTGGAAGGCA TCGCAGTTAT AAATATTCCA AGCATGCATG GGGGTTCCAA CCTATGGGGA
    GAGACTAAGA AACACTGGGG AGAACCTCGC AATGCTTCAT GTCATAATGC CCCTGAAGCC
    ATTACTGACC CTGATGCCTT AAAGTCATGT GTACAAGACC TCAGTGACAA GAGGCTGGAG
    GTAGTAGGAC TTGAGGGTGC TATTGAGATG GGGCAGATTT ATACTGGACT GAAGAGTGCT
    GGCAAACGAC TTGCAACATG TTCTGAACTA ACAATAAGGA CCACAAAGCC CCTCCCAATG
    CAAATTGATG GCGAGCCCTG GATGCAGCCA CCCTGCACGA TAAAAATCAC ACATAAGAAC
    CAGGCACCGA TGCTGCGTGC CCCTGCGCCC CGTTCTTCAA GCTTTTTCTC CCTGAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52684
    Clone ID Related Accession (Same CDS sequence) XM_012957813.3 , XM_012957814.3 , XM_031897165.1 , XM_018091693.2 , XM_012957812.3 , XM_012957808.3 , XM_012957809.3 , XM_012957811.3 , XM_012957810.3
    Accession Version XM_012957813.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2235bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product diacylglycerol kinase alpha
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957813.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCGACG AGAACAAGGA GTGGGCAATC CTGAGCCCGG AGGACTTCAC CCAACTGCAG 
    AAGTACATTG ATTACAGCAC CAAGAAGGTT AAAGATGTTC AGCAAGAGTT CTATGGGGAT
    GGTACATCTT GCCCTCACTT GGTTGAAGAC ACAATAGATT TTGAAGCATT CAAATCCTTT
    TTGAAAACAT ACCTGGAAGT AGATGACATT CCCAACAATC TCTGTGAGCG TCTCTTCATG
    TCTTTTCAAG CAACCCCAAT TGAGAGCAAA AAAAGTGGGG AGGATGAGAC AGAAGGCATA
    AAAGGTAGTG GCCGTATCAG TCTAAATGAT GTGTCCTGTT ATTTCTCTTT GCTGGAAAGT
    GGCACTGCTG AGGATAAACT GGCATTCACC TTTAAATTGT ATGACAAAGA CAGCAATGGT
    CTTCTGGACA GCTCTGAGGT GGACAGGATT ATAACACAAA TGATGCGTGT AGCGGAATAT
    CTGGACTGGG ACATCACGGA ACTCAAACCT ATGTTAGAGG AGATGATGAG ACAAGTGGAC
    TATGATGGAA ATGGAATAGT CTCTTATGAA GAATGGGTTA AAGGAGGACT CACAACAGTG
    CCTCTGCTGG TGCTTCTAGG AATGGAAGTT AATATAAAAG ACGATGGGCA GCACCTGTGG
    CGCCTGAAGC ATTTCAACCG CCCTGTGTAT TGCAATGTGT GTGAAGCTCT TCTTTTTGGG
    GTCAGGAAGC AGGGCCTGTG TTGCACTTTC TGTAAGTTTA CCGTTCATGA ACGCTGTGCC
    CGCAAAGCGC CTAATTCCTG CATAAGCACG TATGCCAAAT CACGCAAGGA TTTCAGCGAG
    CAGGCCCATG TTTGGATGAG AGGTGGATGT GAGGCCAGCA AGTGTGACAA ATGCCAAAAA
    AAAATAAAAA GTTTCCAGAG CCTGACTGGG CAGCGCTGTG TGTGGTGTCA TACAAAGGTT
    CATGATGAAT GTGCCTCATC CCCACTGTGT GATGGTGGAA TCTTAAAGGA CCACATATTA
    CCTCCTGCTT GCATATATCC AGTAATCCTG GAACGACAGA ATAGCCAAAA AAATCTTCTA
    GCTTCCTCAG AAGAAGCATC ACAGACGTTT AGTACCCCAG AAGGACAAGT CCTGCGGATT
    GCCCCATTAC CAGGTACACA CCCTCTACTT GTCTTTGTAA ACCCAAAGAG TGGAGGGAAG
    CAGGGGGAAA GAGTGCTGAG GAAATTCCAG TATCTGCTAA ATCCCAGACA AGTCCATAAC
    TTGATAAAGG GAGGCCCTTC CCCAGGGCTG AATTTTTTCC GGGATGTCCC AGAATATCGT
    ATCTTAGTAT GCGGCGGTGA TGGAACAGTC GGTTGGATCC TGGATGCTAT TGACAAGGCA
    AACCTTCCAT ATAGACCACC TGTAGCTGTG CTGCCCCTCG GTACAGGAAA TGACCTTGCG
    CGATGTCTCA GCTGGGGAGG TGGTTATGAT GGAGAAAATC TAATGAAGTT CTTGAAGGAC
    ATTGAAAAGA GTGTACCCAT TAAAATGGAC AGATGGAACA TTGAGGTTAT ACCTGAAAAC
    CCAGATGAGA AGGGAGACCC AGTTCCTTAC GAGATTATAA ACAACTACTT TTCCATTGGG
    GTGGATGCAT CTATAGCTCA CAGATTCCAT AATATGAGGG AAAAATATCC AGAGAAATTC
    AATAGCAGAA TGAAGAACAA ATTGTGGTAT TTTGAGTTTG CTACATCAGA GACTGTTTTC
    TCTACCTGTA AAAAACTTAA AGAGGTCATT ACAATTGAGT GTTGTGGCTC CCCGTTGAAA
    CTCAGCTCTC TGTCCCTGGA AGGCATCGCA GTTATAAATA TTCCAAGCAT GCATGGGGGT
    TCCAACCTAT GGGGAGAGAC TAAGAAACAC TGGGGAGAAC CTCGCAATGC TTCATGTCAT
    AATGCCCCTG AAGCCATTAC TGACCCTGAT GCCTTAAAGT CATGTGTACA AGACCTCAGT
    GACAAGAGGC TGGAGGTAGT AGGACTTGAG GGTGCTATTG AGATGGGGCA GATTTATACT
    GGACTGAAGA GTGCTGGCAA ACGACTTGCA ACATGTTCTG AACTAACAAT AAGGACCACA
    AAGCCCCTCC CAATGCAAAT TGATGGCGAG CCCTGGATGC AGCCACCCTG CACGATAAAA
    ATCACACATA AGAACCAGGC ACCGATGCTG CGTGCCCCTG CGCCCCGTTC TTCAAGCTTT
    TTCTCCCTGA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813267.1
    CDS581..2815
    Translation

    Target ORF information:

    RefSeq Version XM_012957813.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis diacylglycerol kinase alpha (dgka), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957813.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCGACG AGAACAAGGA GTGGGCAATC CTGAGCCCGG AGGACTTCAC CCAACTGCAG 
    AAGTACATTG ATTACAGCAC CAAGAAGGTT AAAGATGTTC AGCAAGAGTT CTATGGGGAT
    GGTACATCTT GCCCTCACTT GGTTGAAGAC ACAATAGATT TTGAAGCATT CAAATCCTTT
    TTGAAAACAT ACCTGGAAGT AGATGACATT CCCAACAATC TCTGTGAGCG TCTCTTCATG
    TCTTTTCAAG CAACCCCAAT TGAGAGCAAA AAAAGTGGGG AGGATGAGAC AGAAGGCATA
    AAAGGTAGTG GCCGTATCAG TCTAAATGAT GTGTCCTGTT ATTTCTCTTT GCTGGAAAGT
    GGCACTGCTG AGGATAAACT GGCATTCACC TTTAAATTGT ATGACAAAGA CAGCAATGGT
    CTTCTGGACA GCTCTGAGGT GGACAGGATT ATAACACAAA TGATGCGTGT AGCGGAATAT
    CTGGACTGGG ACATCACGGA ACTCAAACCT ATGTTAGAGG AGATGATGAG ACAAGTGGAC
    TATGATGGAA ATGGAATAGT CTCTTATGAA GAATGGGTTA AAGGAGGACT CACAACAGTG
    CCTCTGCTGG TGCTTCTAGG AATGGAAGTT AATATAAAAG ACGATGGGCA GCACCTGTGG
    CGCCTGAAGC ATTTCAACCG CCCTGTGTAT TGCAATGTGT GTGAAGCTCT TCTTTTTGGG
    GTCAGGAAGC AGGGCCTGTG TTGCACTTTC TGTAAGTTTA CCGTTCATGA ACGCTGTGCC
    CGCAAAGCGC CTAATTCCTG CATAAGCACG TATGCCAAAT CACGCAAGGA TTTCAGCGAG
    CAGGCCCATG TTTGGATGAG AGGTGGATGT GAGGCCAGCA AGTGTGACAA ATGCCAAAAA
    AAAATAAAAA GTTTCCAGAG CCTGACTGGG CAGCGCTGTG TGTGGTGTCA TACAAAGGTT
    CATGATGAAT GTGCCTCATC CCCACTGTGT GATGGTGGAA TCTTAAAGGA CCACATATTA
    CCTCCTGCTT GCATATATCC AGTAATCCTG GAACGACAGA ATAGCCAAAA AAATCTTCTA
    GCTTCCTCAG AAGAAGCATC ACAGACGTTT AGTACCCCAG AAGGACAAGT CCTGCGGATT
    GCCCCATTAC CAGGTACACA CCCTCTACTT GTCTTTGTAA ACCCAAAGAG TGGAGGGAAG
    CAGGGGGAAA GAGTGCTGAG GAAATTCCAG TATCTGCTAA ATCCCAGACA AGTCCATAAC
    TTGATAAAGG GAGGCCCTTC CCCAGGGCTG AATTTTTTCC GGGATGTCCC AGAATATCGT
    ATCTTAGTAT GCGGCGGTGA TGGAACAGTC GGTTGGATCC TGGATGCTAT TGACAAGGCA
    AACCTTCCAT ATAGACCACC TGTAGCTGTG CTGCCCCTCG GTACAGGAAA TGACCTTGCG
    CGATGTCTCA GCTGGGGAGG TGGTTATGAT GGAGAAAATC TAATGAAGTT CTTGAAGGAC
    ATTGAAAAGA GTGTACCCAT TAAAATGGAC AGATGGAACA TTGAGGTTAT ACCTGAAAAC
    CCAGATGAGA AGGGAGACCC AGTTCCTTAC GAGATTATAA ACAACTACTT TTCCATTGGG
    GTGGATGCAT CTATAGCTCA CAGATTCCAT AATATGAGGG AAAAATATCC AGAGAAATTC
    AATAGCAGAA TGAAGAACAA ATTGTGGTAT TTTGAGTTTG CTACATCAGA GACTGTTTTC
    TCTACCTGTA AAAAACTTAA AGAGGTCATT ACAATTGAGT GTTGTGGCTC CCCGTTGAAA
    CTCAGCTCTC TGTCCCTGGA AGGCATCGCA GTTATAAATA TTCCAAGCAT GCATGGGGGT
    TCCAACCTAT GGGGAGAGAC TAAGAAACAC TGGGGAGAAC CTCGCAATGC TTCATGTCAT
    AATGCCCCTG AAGCCATTAC TGACCCTGAT GCCTTAAAGT CATGTGTACA AGACCTCAGT
    GACAAGAGGC TGGAGGTAGT AGGACTTGAG GGTGCTATTG AGATGGGGCA GATTTATACT
    GGACTGAAGA GTGCTGGCAA ACGACTTGCA ACATGTTCTG AACTAACAAT AAGGACCACA
    AAGCCCCTCC CAATGCAAAT TGATGGCGAG CCCTGGATGC AGCCACCCTG CACGATAAAA
    ATCACACATA AGAACCAGGC ACCGATGCTG CGTGCCCCTG CGCCCCGTTC TTCAAGCTTT
    TTCTCCCTGA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52684
    Clone ID Related Accession (Same CDS sequence) XM_012957813.3 , XM_012957814.3 , XM_031897165.1 , XM_018091693.2 , XM_012957812.3 , XM_012957808.3 , XM_012957809.3 , XM_012957811.3 , XM_012957810.3
    Accession Version XM_012957814.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2235bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product diacylglycerol kinase alpha
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957814.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCGACG AGAACAAGGA GTGGGCAATC CTGAGCCCGG AGGACTTCAC CCAACTGCAG 
    AAGTACATTG ATTACAGCAC CAAGAAGGTT AAAGATGTTC AGCAAGAGTT CTATGGGGAT
    GGTACATCTT GCCCTCACTT GGTTGAAGAC ACAATAGATT TTGAAGCATT CAAATCCTTT
    TTGAAAACAT ACCTGGAAGT AGATGACATT CCCAACAATC TCTGTGAGCG TCTCTTCATG
    TCTTTTCAAG CAACCCCAAT TGAGAGCAAA AAAAGTGGGG AGGATGAGAC AGAAGGCATA
    AAAGGTAGTG GCCGTATCAG TCTAAATGAT GTGTCCTGTT ATTTCTCTTT GCTGGAAAGT
    GGCACTGCTG AGGATAAACT GGCATTCACC TTTAAATTGT ATGACAAAGA CAGCAATGGT
    CTTCTGGACA GCTCTGAGGT GGACAGGATT ATAACACAAA TGATGCGTGT AGCGGAATAT
    CTGGACTGGG ACATCACGGA ACTCAAACCT ATGTTAGAGG AGATGATGAG ACAAGTGGAC
    TATGATGGAA ATGGAATAGT CTCTTATGAA GAATGGGTTA AAGGAGGACT CACAACAGTG
    CCTCTGCTGG TGCTTCTAGG AATGGAAGTT AATATAAAAG ACGATGGGCA GCACCTGTGG
    CGCCTGAAGC ATTTCAACCG CCCTGTGTAT TGCAATGTGT GTGAAGCTCT TCTTTTTGGG
    GTCAGGAAGC AGGGCCTGTG TTGCACTTTC TGTAAGTTTA CCGTTCATGA ACGCTGTGCC
    CGCAAAGCGC CTAATTCCTG CATAAGCACG TATGCCAAAT CACGCAAGGA TTTCAGCGAG
    CAGGCCCATG TTTGGATGAG AGGTGGATGT GAGGCCAGCA AGTGTGACAA ATGCCAAAAA
    AAAATAAAAA GTTTCCAGAG CCTGACTGGG CAGCGCTGTG TGTGGTGTCA TACAAAGGTT
    CATGATGAAT GTGCCTCATC CCCACTGTGT GATGGTGGAA TCTTAAAGGA CCACATATTA
    CCTCCTGCTT GCATATATCC AGTAATCCTG GAACGACAGA ATAGCCAAAA AAATCTTCTA
    GCTTCCTCAG AAGAAGCATC ACAGACGTTT AGTACCCCAG AAGGACAAGT CCTGCGGATT
    GCCCCATTAC CAGGTACACA CCCTCTACTT GTCTTTGTAA ACCCAAAGAG TGGAGGGAAG
    CAGGGGGAAA GAGTGCTGAG GAAATTCCAG TATCTGCTAA ATCCCAGACA AGTCCATAAC
    TTGATAAAGG GAGGCCCTTC CCCAGGGCTG AATTTTTTCC GGGATGTCCC AGAATATCGT
    ATCTTAGTAT GCGGCGGTGA TGGAACAGTC GGTTGGATCC TGGATGCTAT TGACAAGGCA
    AACCTTCCAT ATAGACCACC TGTAGCTGTG CTGCCCCTCG GTACAGGAAA TGACCTTGCG
    CGATGTCTCA GCTGGGGAGG TGGTTATGAT GGAGAAAATC TAATGAAGTT CTTGAAGGAC
    ATTGAAAAGA GTGTACCCAT TAAAATGGAC AGATGGAACA TTGAGGTTAT ACCTGAAAAC
    CCAGATGAGA AGGGAGACCC AGTTCCTTAC GAGATTATAA ACAACTACTT TTCCATTGGG
    GTGGATGCAT CTATAGCTCA CAGATTCCAT AATATGAGGG AAAAATATCC AGAGAAATTC
    AATAGCAGAA TGAAGAACAA ATTGTGGTAT TTTGAGTTTG CTACATCAGA GACTGTTTTC
    TCTACCTGTA AAAAACTTAA AGAGGTCATT ACAATTGAGT GTTGTGGCTC CCCGTTGAAA
    CTCAGCTCTC TGTCCCTGGA AGGCATCGCA GTTATAAATA TTCCAAGCAT GCATGGGGGT
    TCCAACCTAT GGGGAGAGAC TAAGAAACAC TGGGGAGAAC CTCGCAATGC TTCATGTCAT
    AATGCCCCTG AAGCCATTAC TGACCCTGAT GCCTTAAAGT CATGTGTACA AGACCTCAGT
    GACAAGAGGC TGGAGGTAGT AGGACTTGAG GGTGCTATTG AGATGGGGCA GATTTATACT
    GGACTGAAGA GTGCTGGCAA ACGACTTGCA ACATGTTCTG AACTAACAAT AAGGACCACA
    AAGCCCCTCC CAATGCAAAT TGATGGCGAG CCCTGGATGC AGCCACCCTG CACGATAAAA
    ATCACACATA AGAACCAGGC ACCGATGCTG CGTGCCCCTG CGCCCCGTTC TTCAAGCTTT
    TTCTCCCTGA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813268.1
    CDS99..2333
    Translation

    Target ORF information:

    RefSeq Version XM_012957814.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis diacylglycerol kinase alpha (dgka), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957814.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCGACG AGAACAAGGA GTGGGCAATC CTGAGCCCGG AGGACTTCAC CCAACTGCAG 
    AAGTACATTG ATTACAGCAC CAAGAAGGTT AAAGATGTTC AGCAAGAGTT CTATGGGGAT
    GGTACATCTT GCCCTCACTT GGTTGAAGAC ACAATAGATT TTGAAGCATT CAAATCCTTT
    TTGAAAACAT ACCTGGAAGT AGATGACATT CCCAACAATC TCTGTGAGCG TCTCTTCATG
    TCTTTTCAAG CAACCCCAAT TGAGAGCAAA AAAAGTGGGG AGGATGAGAC AGAAGGCATA
    AAAGGTAGTG GCCGTATCAG TCTAAATGAT GTGTCCTGTT ATTTCTCTTT GCTGGAAAGT
    GGCACTGCTG AGGATAAACT GGCATTCACC TTTAAATTGT ATGACAAAGA CAGCAATGGT
    CTTCTGGACA GCTCTGAGGT GGACAGGATT ATAACACAAA TGATGCGTGT AGCGGAATAT
    CTGGACTGGG ACATCACGGA ACTCAAACCT ATGTTAGAGG AGATGATGAG ACAAGTGGAC
    TATGATGGAA ATGGAATAGT CTCTTATGAA GAATGGGTTA AAGGAGGACT CACAACAGTG
    CCTCTGCTGG TGCTTCTAGG AATGGAAGTT AATATAAAAG ACGATGGGCA GCACCTGTGG
    CGCCTGAAGC ATTTCAACCG CCCTGTGTAT TGCAATGTGT GTGAAGCTCT TCTTTTTGGG
    GTCAGGAAGC AGGGCCTGTG TTGCACTTTC TGTAAGTTTA CCGTTCATGA ACGCTGTGCC
    CGCAAAGCGC CTAATTCCTG CATAAGCACG TATGCCAAAT CACGCAAGGA TTTCAGCGAG
    CAGGCCCATG TTTGGATGAG AGGTGGATGT GAGGCCAGCA AGTGTGACAA ATGCCAAAAA
    AAAATAAAAA GTTTCCAGAG CCTGACTGGG CAGCGCTGTG TGTGGTGTCA TACAAAGGTT
    CATGATGAAT GTGCCTCATC CCCACTGTGT GATGGTGGAA TCTTAAAGGA CCACATATTA
    CCTCCTGCTT GCATATATCC AGTAATCCTG GAACGACAGA ATAGCCAAAA AAATCTTCTA
    GCTTCCTCAG AAGAAGCATC ACAGACGTTT AGTACCCCAG AAGGACAAGT CCTGCGGATT
    GCCCCATTAC CAGGTACACA CCCTCTACTT GTCTTTGTAA ACCCAAAGAG TGGAGGGAAG
    CAGGGGGAAA GAGTGCTGAG GAAATTCCAG TATCTGCTAA ATCCCAGACA AGTCCATAAC
    TTGATAAAGG GAGGCCCTTC CCCAGGGCTG AATTTTTTCC GGGATGTCCC AGAATATCGT
    ATCTTAGTAT GCGGCGGTGA TGGAACAGTC GGTTGGATCC TGGATGCTAT TGACAAGGCA
    AACCTTCCAT ATAGACCACC TGTAGCTGTG CTGCCCCTCG GTACAGGAAA TGACCTTGCG
    CGATGTCTCA GCTGGGGAGG TGGTTATGAT GGAGAAAATC TAATGAAGTT CTTGAAGGAC
    ATTGAAAAGA GTGTACCCAT TAAAATGGAC AGATGGAACA TTGAGGTTAT ACCTGAAAAC
    CCAGATGAGA AGGGAGACCC AGTTCCTTAC GAGATTATAA ACAACTACTT TTCCATTGGG
    GTGGATGCAT CTATAGCTCA CAGATTCCAT AATATGAGGG AAAAATATCC AGAGAAATTC
    AATAGCAGAA TGAAGAACAA ATTGTGGTAT TTTGAGTTTG CTACATCAGA GACTGTTTTC
    TCTACCTGTA AAAAACTTAA AGAGGTCATT ACAATTGAGT GTTGTGGCTC CCCGTTGAAA
    CTCAGCTCTC TGTCCCTGGA AGGCATCGCA GTTATAAATA TTCCAAGCAT GCATGGGGGT
    TCCAACCTAT GGGGAGAGAC TAAGAAACAC TGGGGAGAAC CTCGCAATGC TTCATGTCAT
    AATGCCCCTG AAGCCATTAC TGACCCTGAT GCCTTAAAGT CATGTGTACA AGACCTCAGT
    GACAAGAGGC TGGAGGTAGT AGGACTTGAG GGTGCTATTG AGATGGGGCA GATTTATACT
    GGACTGAAGA GTGCTGGCAA ACGACTTGCA ACATGTTCTG AACTAACAAT AAGGACCACA
    AAGCCCCTCC CAATGCAAAT TGATGGCGAG CCCTGGATGC AGCCACCCTG CACGATAAAA
    ATCACACATA AGAACCAGGC ACCGATGCTG CGTGCCCCTG CGCCCCGTTC TTCAAGCTTT
    TTCTCCCTGA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52684
    Clone ID Related Accession (Same CDS sequence) XM_012957813.3 , XM_012957814.3 , XM_031897165.1 , XM_018091693.2 , XM_012957812.3 , XM_012957808.3 , XM_012957809.3 , XM_012957811.3 , XM_012957810.3
    Accession Version XM_031897165.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2235bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product diacylglycerol kinase alpha
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCGACG AGAACAAGGA GTGGGCAATC CTGAGCCCGG AGGACTTCAC CCAACTGCAG 
    AAGTACATTG ATTACAGCAC CAAGAAGGTT AAAGATGTTC AGCAAGAGTT CTATGGGGAT
    GGTACATCTT GCCCTCACTT GGTTGAAGAC ACAATAGATT TTGAAGCATT CAAATCCTTT
    TTGAAAACAT ACCTGGAAGT AGATGACATT CCCAACAATC TCTGTGAGCG TCTCTTCATG
    TCTTTTCAAG CAACCCCAAT TGAGAGCAAA AAAAGTGGGG AGGATGAGAC AGAAGGCATA
    AAAGGTAGTG GCCGTATCAG TCTAAATGAT GTGTCCTGTT ATTTCTCTTT GCTGGAAAGT
    GGCACTGCTG AGGATAAACT GGCATTCACC TTTAAATTGT ATGACAAAGA CAGCAATGGT
    CTTCTGGACA GCTCTGAGGT GGACAGGATT ATAACACAAA TGATGCGTGT AGCGGAATAT
    CTGGACTGGG ACATCACGGA ACTCAAACCT ATGTTAGAGG AGATGATGAG ACAAGTGGAC
    TATGATGGAA ATGGAATAGT CTCTTATGAA GAATGGGTTA AAGGAGGACT CACAACAGTG
    CCTCTGCTGG TGCTTCTAGG AATGGAAGTT AATATAAAAG ACGATGGGCA GCACCTGTGG
    CGCCTGAAGC ATTTCAACCG CCCTGTGTAT TGCAATGTGT GTGAAGCTCT TCTTTTTGGG
    GTCAGGAAGC AGGGCCTGTG TTGCACTTTC TGTAAGTTTA CCGTTCATGA ACGCTGTGCC
    CGCAAAGCGC CTAATTCCTG CATAAGCACG TATGCCAAAT CACGCAAGGA TTTCAGCGAG
    CAGGCCCATG TTTGGATGAG AGGTGGATGT GAGGCCAGCA AGTGTGACAA ATGCCAAAAA
    AAAATAAAAA GTTTCCAGAG CCTGACTGGG CAGCGCTGTG TGTGGTGTCA TACAAAGGTT
    CATGATGAAT GTGCCTCATC CCCACTGTGT GATGGTGGAA TCTTAAAGGA CCACATATTA
    CCTCCTGCTT GCATATATCC AGTAATCCTG GAACGACAGA ATAGCCAAAA AAATCTTCTA
    GCTTCCTCAG AAGAAGCATC ACAGACGTTT AGTACCCCAG AAGGACAAGT CCTGCGGATT
    GCCCCATTAC CAGGTACACA CCCTCTACTT GTCTTTGTAA ACCCAAAGAG TGGAGGGAAG
    CAGGGGGAAA GAGTGCTGAG GAAATTCCAG TATCTGCTAA ATCCCAGACA AGTCCATAAC
    TTGATAAAGG GAGGCCCTTC CCCAGGGCTG AATTTTTTCC GGGATGTCCC AGAATATCGT
    ATCTTAGTAT GCGGCGGTGA TGGAACAGTC GGTTGGATCC TGGATGCTAT TGACAAGGCA
    AACCTTCCAT ATAGACCACC TGTAGCTGTG CTGCCCCTCG GTACAGGAAA TGACCTTGCG
    CGATGTCTCA GCTGGGGAGG TGGTTATGAT GGAGAAAATC TAATGAAGTT CTTGAAGGAC
    ATTGAAAAGA GTGTACCCAT TAAAATGGAC AGATGGAACA TTGAGGTTAT ACCTGAAAAC
    CCAGATGAGA AGGGAGACCC AGTTCCTTAC GAGATTATAA ACAACTACTT TTCCATTGGG
    GTGGATGCAT CTATAGCTCA CAGATTCCAT AATATGAGGG AAAAATATCC AGAGAAATTC
    AATAGCAGAA TGAAGAACAA ATTGTGGTAT TTTGAGTTTG CTACATCAGA GACTGTTTTC
    TCTACCTGTA AAAAACTTAA AGAGGTCATT ACAATTGAGT GTTGTGGCTC CCCGTTGAAA
    CTCAGCTCTC TGTCCCTGGA AGGCATCGCA GTTATAAATA TTCCAAGCAT GCATGGGGGT
    TCCAACCTAT GGGGAGAGAC TAAGAAACAC TGGGGAGAAC CTCGCAATGC TTCATGTCAT
    AATGCCCCTG AAGCCATTAC TGACCCTGAT GCCTTAAAGT CATGTGTACA AGACCTCAGT
    GACAAGAGGC TGGAGGTAGT AGGACTTGAG GGTGCTATTG AGATGGGGCA GATTTATACT
    GGACTGAAGA GTGCTGGCAA ACGACTTGCA ACATGTTCTG AACTAACAAT AAGGACCACA
    AAGCCCCTCC CAATGCAAAT TGATGGCGAG CCCTGGATGC AGCCACCCTG CACGATAAAA
    ATCACACATA AGAACCAGGC ACCGATGCTG CGTGCCCCTG CGCCCCGTTC TTCAAGCTTT
    TTCTCCCTGA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753025.1
    CDS314..2548
    Translation

    Target ORF information:

    RefSeq Version XM_031897165.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis diacylglycerol kinase alpha (dgka), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897165.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCGACG AGAACAAGGA GTGGGCAATC CTGAGCCCGG AGGACTTCAC CCAACTGCAG 
    AAGTACATTG ATTACAGCAC CAAGAAGGTT AAAGATGTTC AGCAAGAGTT CTATGGGGAT
    GGTACATCTT GCCCTCACTT GGTTGAAGAC ACAATAGATT TTGAAGCATT CAAATCCTTT
    TTGAAAACAT ACCTGGAAGT AGATGACATT CCCAACAATC TCTGTGAGCG TCTCTTCATG
    TCTTTTCAAG CAACCCCAAT TGAGAGCAAA AAAAGTGGGG AGGATGAGAC AGAAGGCATA
    AAAGGTAGTG GCCGTATCAG TCTAAATGAT GTGTCCTGTT ATTTCTCTTT GCTGGAAAGT
    GGCACTGCTG AGGATAAACT GGCATTCACC TTTAAATTGT ATGACAAAGA CAGCAATGGT
    CTTCTGGACA GCTCTGAGGT GGACAGGATT ATAACACAAA TGATGCGTGT AGCGGAATAT
    CTGGACTGGG ACATCACGGA ACTCAAACCT ATGTTAGAGG AGATGATGAG ACAAGTGGAC
    TATGATGGAA ATGGAATAGT CTCTTATGAA GAATGGGTTA AAGGAGGACT CACAACAGTG
    CCTCTGCTGG TGCTTCTAGG AATGGAAGTT AATATAAAAG ACGATGGGCA GCACCTGTGG
    CGCCTGAAGC ATTTCAACCG CCCTGTGTAT TGCAATGTGT GTGAAGCTCT TCTTTTTGGG
    GTCAGGAAGC AGGGCCTGTG TTGCACTTTC TGTAAGTTTA CCGTTCATGA ACGCTGTGCC
    CGCAAAGCGC CTAATTCCTG CATAAGCACG TATGCCAAAT CACGCAAGGA TTTCAGCGAG
    CAGGCCCATG TTTGGATGAG AGGTGGATGT GAGGCCAGCA AGTGTGACAA ATGCCAAAAA
    AAAATAAAAA GTTTCCAGAG CCTGACTGGG CAGCGCTGTG TGTGGTGTCA TACAAAGGTT
    CATGATGAAT GTGCCTCATC CCCACTGTGT GATGGTGGAA TCTTAAAGGA CCACATATTA
    CCTCCTGCTT GCATATATCC AGTAATCCTG GAACGACAGA ATAGCCAAAA AAATCTTCTA
    GCTTCCTCAG AAGAAGCATC ACAGACGTTT AGTACCCCAG AAGGACAAGT CCTGCGGATT
    GCCCCATTAC CAGGTACACA CCCTCTACTT GTCTTTGTAA ACCCAAAGAG TGGAGGGAAG
    CAGGGGGAAA GAGTGCTGAG GAAATTCCAG TATCTGCTAA ATCCCAGACA AGTCCATAAC
    TTGATAAAGG GAGGCCCTTC CCCAGGGCTG AATTTTTTCC GGGATGTCCC AGAATATCGT
    ATCTTAGTAT GCGGCGGTGA TGGAACAGTC GGTTGGATCC TGGATGCTAT TGACAAGGCA
    AACCTTCCAT ATAGACCACC TGTAGCTGTG CTGCCCCTCG GTACAGGAAA TGACCTTGCG
    CGATGTCTCA GCTGGGGAGG TGGTTATGAT GGAGAAAATC TAATGAAGTT CTTGAAGGAC
    ATTGAAAAGA GTGTACCCAT TAAAATGGAC AGATGGAACA TTGAGGTTAT ACCTGAAAAC
    CCAGATGAGA AGGGAGACCC AGTTCCTTAC GAGATTATAA ACAACTACTT TTCCATTGGG
    GTGGATGCAT CTATAGCTCA CAGATTCCAT AATATGAGGG AAAAATATCC AGAGAAATTC
    AATAGCAGAA TGAAGAACAA ATTGTGGTAT TTTGAGTTTG CTACATCAGA GACTGTTTTC
    TCTACCTGTA AAAAACTTAA AGAGGTCATT ACAATTGAGT GTTGTGGCTC CCCGTTGAAA
    CTCAGCTCTC TGTCCCTGGA AGGCATCGCA GTTATAAATA TTCCAAGCAT GCATGGGGGT
    TCCAACCTAT GGGGAGAGAC TAAGAAACAC TGGGGAGAAC CTCGCAATGC TTCATGTCAT
    AATGCCCCTG AAGCCATTAC TGACCCTGAT GCCTTAAAGT CATGTGTACA AGACCTCAGT
    GACAAGAGGC TGGAGGTAGT AGGACTTGAG GGTGCTATTG AGATGGGGCA GATTTATACT
    GGACTGAAGA GTGCTGGCAA ACGACTTGCA ACATGTTCTG AACTAACAAT AAGGACCACA
    AAGCCCCTCC CAATGCAAAT TGATGGCGAG CCCTGGATGC AGCCACCCTG CACGATAAAA
    ATCACACATA AGAACCAGGC ACCGATGCTG CGTGCCCCTG CGCCCCGTTC TTCAAGCTTT
    TTCTCCCTGA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52684
    Clone ID Related Accession (Same CDS sequence) XM_012957813.3 , XM_012957814.3 , XM_031897165.1 , XM_018091693.2 , XM_012957812.3 , XM_012957808.3 , XM_012957809.3 , XM_012957811.3 , XM_012957810.3
    Accession Version XM_018091693.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2235bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product diacylglycerol kinase alpha
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018091693.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCGACG AGAACAAGGA GTGGGCAATC CTGAGCCCGG AGGACTTCAC CCAACTGCAG 
    AAGTACATTG ATTACAGCAC CAAGAAGGTT AAAGATGTTC AGCAAGAGTT CTATGGGGAT
    GGTACATCTT GCCCTCACTT GGTTGAAGAC ACAATAGATT TTGAAGCATT CAAATCCTTT
    TTGAAAACAT ACCTGGAAGT AGATGACATT CCCAACAATC TCTGTGAGCG TCTCTTCATG
    TCTTTTCAAG CAACCCCAAT TGAGAGCAAA AAAAGTGGGG AGGATGAGAC AGAAGGCATA
    AAAGGTAGTG GCCGTATCAG TCTAAATGAT GTGTCCTGTT ATTTCTCTTT GCTGGAAAGT
    GGCACTGCTG AGGATAAACT GGCATTCACC TTTAAATTGT ATGACAAAGA CAGCAATGGT
    CTTCTGGACA GCTCTGAGGT GGACAGGATT ATAACACAAA TGATGCGTGT AGCGGAATAT
    CTGGACTGGG ACATCACGGA ACTCAAACCT ATGTTAGAGG AGATGATGAG ACAAGTGGAC
    TATGATGGAA ATGGAATAGT CTCTTATGAA GAATGGGTTA AAGGAGGACT CACAACAGTG
    CCTCTGCTGG TGCTTCTAGG AATGGAAGTT AATATAAAAG ACGATGGGCA GCACCTGTGG
    CGCCTGAAGC ATTTCAACCG CCCTGTGTAT TGCAATGTGT GTGAAGCTCT TCTTTTTGGG
    GTCAGGAAGC AGGGCCTGTG TTGCACTTTC TGTAAGTTTA CCGTTCATGA ACGCTGTGCC
    CGCAAAGCGC CTAATTCCTG CATAAGCACG TATGCCAAAT CACGCAAGGA TTTCAGCGAG
    CAGGCCCATG TTTGGATGAG AGGTGGATGT GAGGCCAGCA AGTGTGACAA ATGCCAAAAA
    AAAATAAAAA GTTTCCAGAG CCTGACTGGG CAGCGCTGTG TGTGGTGTCA TACAAAGGTT
    CATGATGAAT GTGCCTCATC CCCACTGTGT GATGGTGGAA TCTTAAAGGA CCACATATTA
    CCTCCTGCTT GCATATATCC AGTAATCCTG GAACGACAGA ATAGCCAAAA AAATCTTCTA
    GCTTCCTCAG AAGAAGCATC ACAGACGTTT AGTACCCCAG AAGGACAAGT CCTGCGGATT
    GCCCCATTAC CAGGTACACA CCCTCTACTT GTCTTTGTAA ACCCAAAGAG TGGAGGGAAG
    CAGGGGGAAA GAGTGCTGAG GAAATTCCAG TATCTGCTAA ATCCCAGACA AGTCCATAAC
    TTGATAAAGG GAGGCCCTTC CCCAGGGCTG AATTTTTTCC GGGATGTCCC AGAATATCGT
    ATCTTAGTAT GCGGCGGTGA TGGAACAGTC GGTTGGATCC TGGATGCTAT TGACAAGGCA
    AACCTTCCAT ATAGACCACC TGTAGCTGTG CTGCCCCTCG GTACAGGAAA TGACCTTGCG
    CGATGTCTCA GCTGGGGAGG TGGTTATGAT GGAGAAAATC TAATGAAGTT CTTGAAGGAC
    ATTGAAAAGA GTGTACCCAT TAAAATGGAC AGATGGAACA TTGAGGTTAT ACCTGAAAAC
    CCAGATGAGA AGGGAGACCC AGTTCCTTAC GAGATTATAA ACAACTACTT TTCCATTGGG
    GTGGATGCAT CTATAGCTCA CAGATTCCAT AATATGAGGG AAAAATATCC AGAGAAATTC
    AATAGCAGAA TGAAGAACAA ATTGTGGTAT TTTGAGTTTG CTACATCAGA GACTGTTTTC
    TCTACCTGTA AAAAACTTAA AGAGGTCATT ACAATTGAGT GTTGTGGCTC CCCGTTGAAA
    CTCAGCTCTC TGTCCCTGGA AGGCATCGCA GTTATAAATA TTCCAAGCAT GCATGGGGGT
    TCCAACCTAT GGGGAGAGAC TAAGAAACAC TGGGGAGAAC CTCGCAATGC TTCATGTCAT
    AATGCCCCTG AAGCCATTAC TGACCCTGAT GCCTTAAAGT CATGTGTACA AGACCTCAGT
    GACAAGAGGC TGGAGGTAGT AGGACTTGAG GGTGCTATTG AGATGGGGCA GATTTATACT
    GGACTGAAGA GTGCTGGCAA ACGACTTGCA ACATGTTCTG AACTAACAAT AAGGACCACA
    AAGCCCCTCC CAATGCAAAT TGATGGCGAG CCCTGGATGC AGCCACCCTG CACGATAAAA
    ATCACACATA AGAACCAGGC ACCGATGCTG CGTGCCCCTG CGCCCCGTTC TTCAAGCTTT
    TTCTCCCTGA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947182.1
    CDS445..2679
    Translation

    Target ORF information:

    RefSeq Version XM_018091693.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis diacylglycerol kinase alpha (dgka), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091693.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCGACG AGAACAAGGA GTGGGCAATC CTGAGCCCGG AGGACTTCAC CCAACTGCAG 
    AAGTACATTG ATTACAGCAC CAAGAAGGTT AAAGATGTTC AGCAAGAGTT CTATGGGGAT
    GGTACATCTT GCCCTCACTT GGTTGAAGAC ACAATAGATT TTGAAGCATT CAAATCCTTT
    TTGAAAACAT ACCTGGAAGT AGATGACATT CCCAACAATC TCTGTGAGCG TCTCTTCATG
    TCTTTTCAAG CAACCCCAAT TGAGAGCAAA AAAAGTGGGG AGGATGAGAC AGAAGGCATA
    AAAGGTAGTG GCCGTATCAG TCTAAATGAT GTGTCCTGTT ATTTCTCTTT GCTGGAAAGT
    GGCACTGCTG AGGATAAACT GGCATTCACC TTTAAATTGT ATGACAAAGA CAGCAATGGT
    CTTCTGGACA GCTCTGAGGT GGACAGGATT ATAACACAAA TGATGCGTGT AGCGGAATAT
    CTGGACTGGG ACATCACGGA ACTCAAACCT ATGTTAGAGG AGATGATGAG ACAAGTGGAC
    TATGATGGAA ATGGAATAGT CTCTTATGAA GAATGGGTTA AAGGAGGACT CACAACAGTG
    CCTCTGCTGG TGCTTCTAGG AATGGAAGTT AATATAAAAG ACGATGGGCA GCACCTGTGG
    CGCCTGAAGC ATTTCAACCG CCCTGTGTAT TGCAATGTGT GTGAAGCTCT TCTTTTTGGG
    GTCAGGAAGC AGGGCCTGTG TTGCACTTTC TGTAAGTTTA CCGTTCATGA ACGCTGTGCC
    CGCAAAGCGC CTAATTCCTG CATAAGCACG TATGCCAAAT CACGCAAGGA TTTCAGCGAG
    CAGGCCCATG TTTGGATGAG AGGTGGATGT GAGGCCAGCA AGTGTGACAA ATGCCAAAAA
    AAAATAAAAA GTTTCCAGAG CCTGACTGGG CAGCGCTGTG TGTGGTGTCA TACAAAGGTT
    CATGATGAAT GTGCCTCATC CCCACTGTGT GATGGTGGAA TCTTAAAGGA CCACATATTA
    CCTCCTGCTT GCATATATCC AGTAATCCTG GAACGACAGA ATAGCCAAAA AAATCTTCTA
    GCTTCCTCAG AAGAAGCATC ACAGACGTTT AGTACCCCAG AAGGACAAGT CCTGCGGATT
    GCCCCATTAC CAGGTACACA CCCTCTACTT GTCTTTGTAA ACCCAAAGAG TGGAGGGAAG
    CAGGGGGAAA GAGTGCTGAG GAAATTCCAG TATCTGCTAA ATCCCAGACA AGTCCATAAC
    TTGATAAAGG GAGGCCCTTC CCCAGGGCTG AATTTTTTCC GGGATGTCCC AGAATATCGT
    ATCTTAGTAT GCGGCGGTGA TGGAACAGTC GGTTGGATCC TGGATGCTAT TGACAAGGCA
    AACCTTCCAT ATAGACCACC TGTAGCTGTG CTGCCCCTCG GTACAGGAAA TGACCTTGCG
    CGATGTCTCA GCTGGGGAGG TGGTTATGAT GGAGAAAATC TAATGAAGTT CTTGAAGGAC
    ATTGAAAAGA GTGTACCCAT TAAAATGGAC AGATGGAACA TTGAGGTTAT ACCTGAAAAC
    CCAGATGAGA AGGGAGACCC AGTTCCTTAC GAGATTATAA ACAACTACTT TTCCATTGGG
    GTGGATGCAT CTATAGCTCA CAGATTCCAT AATATGAGGG AAAAATATCC AGAGAAATTC
    AATAGCAGAA TGAAGAACAA ATTGTGGTAT TTTGAGTTTG CTACATCAGA GACTGTTTTC
    TCTACCTGTA AAAAACTTAA AGAGGTCATT ACAATTGAGT GTTGTGGCTC CCCGTTGAAA
    CTCAGCTCTC TGTCCCTGGA AGGCATCGCA GTTATAAATA TTCCAAGCAT GCATGGGGGT
    TCCAACCTAT GGGGAGAGAC TAAGAAACAC TGGGGAGAAC CTCGCAATGC TTCATGTCAT
    AATGCCCCTG AAGCCATTAC TGACCCTGAT GCCTTAAAGT CATGTGTACA AGACCTCAGT
    GACAAGAGGC TGGAGGTAGT AGGACTTGAG GGTGCTATTG AGATGGGGCA GATTTATACT
    GGACTGAAGA GTGCTGGCAA ACGACTTGCA ACATGTTCTG AACTAACAAT AAGGACCACA
    AAGCCCCTCC CAATGCAAAT TGATGGCGAG CCCTGGATGC AGCCACCCTG CACGATAAAA
    ATCACACATA AGAACCAGGC ACCGATGCTG CGTGCCCCTG CGCCCCGTTC TTCAAGCTTT
    TTCTCCCTGA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52684
    Clone ID Related Accession (Same CDS sequence) XM_012957813.3 , XM_012957814.3 , XM_031897165.1 , XM_018091693.2 , XM_012957812.3 , XM_012957808.3 , XM_012957809.3 , XM_012957811.3 , XM_012957810.3
    Accession Version XM_012957812.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2235bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product diacylglycerol kinase alpha
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957812.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCGACG AGAACAAGGA GTGGGCAATC CTGAGCCCGG AGGACTTCAC CCAACTGCAG 
    AAGTACATTG ATTACAGCAC CAAGAAGGTT AAAGATGTTC AGCAAGAGTT CTATGGGGAT
    GGTACATCTT GCCCTCACTT GGTTGAAGAC ACAATAGATT TTGAAGCATT CAAATCCTTT
    TTGAAAACAT ACCTGGAAGT AGATGACATT CCCAACAATC TCTGTGAGCG TCTCTTCATG
    TCTTTTCAAG CAACCCCAAT TGAGAGCAAA AAAAGTGGGG AGGATGAGAC AGAAGGCATA
    AAAGGTAGTG GCCGTATCAG TCTAAATGAT GTGTCCTGTT ATTTCTCTTT GCTGGAAAGT
    GGCACTGCTG AGGATAAACT GGCATTCACC TTTAAATTGT ATGACAAAGA CAGCAATGGT
    CTTCTGGACA GCTCTGAGGT GGACAGGATT ATAACACAAA TGATGCGTGT AGCGGAATAT
    CTGGACTGGG ACATCACGGA ACTCAAACCT ATGTTAGAGG AGATGATGAG ACAAGTGGAC
    TATGATGGAA ATGGAATAGT CTCTTATGAA GAATGGGTTA AAGGAGGACT CACAACAGTG
    CCTCTGCTGG TGCTTCTAGG AATGGAAGTT AATATAAAAG ACGATGGGCA GCACCTGTGG
    CGCCTGAAGC ATTTCAACCG CCCTGTGTAT TGCAATGTGT GTGAAGCTCT TCTTTTTGGG
    GTCAGGAAGC AGGGCCTGTG TTGCACTTTC TGTAAGTTTA CCGTTCATGA ACGCTGTGCC
    CGCAAAGCGC CTAATTCCTG CATAAGCACG TATGCCAAAT CACGCAAGGA TTTCAGCGAG
    CAGGCCCATG TTTGGATGAG AGGTGGATGT GAGGCCAGCA AGTGTGACAA ATGCCAAAAA
    AAAATAAAAA GTTTCCAGAG CCTGACTGGG CAGCGCTGTG TGTGGTGTCA TACAAAGGTT
    CATGATGAAT GTGCCTCATC CCCACTGTGT GATGGTGGAA TCTTAAAGGA CCACATATTA
    CCTCCTGCTT GCATATATCC AGTAATCCTG GAACGACAGA ATAGCCAAAA AAATCTTCTA
    GCTTCCTCAG AAGAAGCATC ACAGACGTTT AGTACCCCAG AAGGACAAGT CCTGCGGATT
    GCCCCATTAC CAGGTACACA CCCTCTACTT GTCTTTGTAA ACCCAAAGAG TGGAGGGAAG
    CAGGGGGAAA GAGTGCTGAG GAAATTCCAG TATCTGCTAA ATCCCAGACA AGTCCATAAC
    TTGATAAAGG GAGGCCCTTC CCCAGGGCTG AATTTTTTCC GGGATGTCCC AGAATATCGT
    ATCTTAGTAT GCGGCGGTGA TGGAACAGTC GGTTGGATCC TGGATGCTAT TGACAAGGCA
    AACCTTCCAT ATAGACCACC TGTAGCTGTG CTGCCCCTCG GTACAGGAAA TGACCTTGCG
    CGATGTCTCA GCTGGGGAGG TGGTTATGAT GGAGAAAATC TAATGAAGTT CTTGAAGGAC
    ATTGAAAAGA GTGTACCCAT TAAAATGGAC AGATGGAACA TTGAGGTTAT ACCTGAAAAC
    CCAGATGAGA AGGGAGACCC AGTTCCTTAC GAGATTATAA ACAACTACTT TTCCATTGGG
    GTGGATGCAT CTATAGCTCA CAGATTCCAT AATATGAGGG AAAAATATCC AGAGAAATTC
    AATAGCAGAA TGAAGAACAA ATTGTGGTAT TTTGAGTTTG CTACATCAGA GACTGTTTTC
    TCTACCTGTA AAAAACTTAA AGAGGTCATT ACAATTGAGT GTTGTGGCTC CCCGTTGAAA
    CTCAGCTCTC TGTCCCTGGA AGGCATCGCA GTTATAAATA TTCCAAGCAT GCATGGGGGT
    TCCAACCTAT GGGGAGAGAC TAAGAAACAC TGGGGAGAAC CTCGCAATGC TTCATGTCAT
    AATGCCCCTG AAGCCATTAC TGACCCTGAT GCCTTAAAGT CATGTGTACA AGACCTCAGT
    GACAAGAGGC TGGAGGTAGT AGGACTTGAG GGTGCTATTG AGATGGGGCA GATTTATACT
    GGACTGAAGA GTGCTGGCAA ACGACTTGCA ACATGTTCTG AACTAACAAT AAGGACCACA
    AAGCCCCTCC CAATGCAAAT TGATGGCGAG CCCTGGATGC AGCCACCCTG CACGATAAAA
    ATCACACATA AGAACCAGGC ACCGATGCTG CGTGCCCCTG CGCCCCGTTC TTCAAGCTTT
    TTCTCCCTGA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813266.1
    CDS363..2597
    Translation

    Target ORF information:

    RefSeq Version XM_012957812.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis diacylglycerol kinase alpha (dgka), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957812.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCGACG AGAACAAGGA GTGGGCAATC CTGAGCCCGG AGGACTTCAC CCAACTGCAG 
    AAGTACATTG ATTACAGCAC CAAGAAGGTT AAAGATGTTC AGCAAGAGTT CTATGGGGAT
    GGTACATCTT GCCCTCACTT GGTTGAAGAC ACAATAGATT TTGAAGCATT CAAATCCTTT
    TTGAAAACAT ACCTGGAAGT AGATGACATT CCCAACAATC TCTGTGAGCG TCTCTTCATG
    TCTTTTCAAG CAACCCCAAT TGAGAGCAAA AAAAGTGGGG AGGATGAGAC AGAAGGCATA
    AAAGGTAGTG GCCGTATCAG TCTAAATGAT GTGTCCTGTT ATTTCTCTTT GCTGGAAAGT
    GGCACTGCTG AGGATAAACT GGCATTCACC TTTAAATTGT ATGACAAAGA CAGCAATGGT
    CTTCTGGACA GCTCTGAGGT GGACAGGATT ATAACACAAA TGATGCGTGT AGCGGAATAT
    CTGGACTGGG ACATCACGGA ACTCAAACCT ATGTTAGAGG AGATGATGAG ACAAGTGGAC
    TATGATGGAA ATGGAATAGT CTCTTATGAA GAATGGGTTA AAGGAGGACT CACAACAGTG
    CCTCTGCTGG TGCTTCTAGG AATGGAAGTT AATATAAAAG ACGATGGGCA GCACCTGTGG
    CGCCTGAAGC ATTTCAACCG CCCTGTGTAT TGCAATGTGT GTGAAGCTCT TCTTTTTGGG
    GTCAGGAAGC AGGGCCTGTG TTGCACTTTC TGTAAGTTTA CCGTTCATGA ACGCTGTGCC
    CGCAAAGCGC CTAATTCCTG CATAAGCACG TATGCCAAAT CACGCAAGGA TTTCAGCGAG
    CAGGCCCATG TTTGGATGAG AGGTGGATGT GAGGCCAGCA AGTGTGACAA ATGCCAAAAA
    AAAATAAAAA GTTTCCAGAG CCTGACTGGG CAGCGCTGTG TGTGGTGTCA TACAAAGGTT
    CATGATGAAT GTGCCTCATC CCCACTGTGT GATGGTGGAA TCTTAAAGGA CCACATATTA
    CCTCCTGCTT GCATATATCC AGTAATCCTG GAACGACAGA ATAGCCAAAA AAATCTTCTA
    GCTTCCTCAG AAGAAGCATC ACAGACGTTT AGTACCCCAG AAGGACAAGT CCTGCGGATT
    GCCCCATTAC CAGGTACACA CCCTCTACTT GTCTTTGTAA ACCCAAAGAG TGGAGGGAAG
    CAGGGGGAAA GAGTGCTGAG GAAATTCCAG TATCTGCTAA ATCCCAGACA AGTCCATAAC
    TTGATAAAGG GAGGCCCTTC CCCAGGGCTG AATTTTTTCC GGGATGTCCC AGAATATCGT
    ATCTTAGTAT GCGGCGGTGA TGGAACAGTC GGTTGGATCC TGGATGCTAT TGACAAGGCA
    AACCTTCCAT ATAGACCACC TGTAGCTGTG CTGCCCCTCG GTACAGGAAA TGACCTTGCG
    CGATGTCTCA GCTGGGGAGG TGGTTATGAT GGAGAAAATC TAATGAAGTT CTTGAAGGAC
    ATTGAAAAGA GTGTACCCAT TAAAATGGAC AGATGGAACA TTGAGGTTAT ACCTGAAAAC
    CCAGATGAGA AGGGAGACCC AGTTCCTTAC GAGATTATAA ACAACTACTT TTCCATTGGG
    GTGGATGCAT CTATAGCTCA CAGATTCCAT AATATGAGGG AAAAATATCC AGAGAAATTC
    AATAGCAGAA TGAAGAACAA ATTGTGGTAT TTTGAGTTTG CTACATCAGA GACTGTTTTC
    TCTACCTGTA AAAAACTTAA AGAGGTCATT ACAATTGAGT GTTGTGGCTC CCCGTTGAAA
    CTCAGCTCTC TGTCCCTGGA AGGCATCGCA GTTATAAATA TTCCAAGCAT GCATGGGGGT
    TCCAACCTAT GGGGAGAGAC TAAGAAACAC TGGGGAGAAC CTCGCAATGC TTCATGTCAT
    AATGCCCCTG AAGCCATTAC TGACCCTGAT GCCTTAAAGT CATGTGTACA AGACCTCAGT
    GACAAGAGGC TGGAGGTAGT AGGACTTGAG GGTGCTATTG AGATGGGGCA GATTTATACT
    GGACTGAAGA GTGCTGGCAA ACGACTTGCA ACATGTTCTG AACTAACAAT AAGGACCACA
    AAGCCCCTCC CAATGCAAAT TGATGGCGAG CCCTGGATGC AGCCACCCTG CACGATAAAA
    ATCACACATA AGAACCAGGC ACCGATGCTG CGTGCCCCTG CGCCCCGTTC TTCAAGCTTT
    TTCTCCCTGA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52684
    Clone ID Related Accession (Same CDS sequence) XM_012957813.3 , XM_012957814.3 , XM_031897165.1 , XM_018091693.2 , XM_012957812.3 , XM_012957808.3 , XM_012957809.3 , XM_012957811.3 , XM_012957810.3
    Accession Version XM_012957808.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2235bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product diacylglycerol kinase alpha
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957808.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCGACG AGAACAAGGA GTGGGCAATC CTGAGCCCGG AGGACTTCAC CCAACTGCAG 
    AAGTACATTG ATTACAGCAC CAAGAAGGTT AAAGATGTTC AGCAAGAGTT CTATGGGGAT
    GGTACATCTT GCCCTCACTT GGTTGAAGAC ACAATAGATT TTGAAGCATT CAAATCCTTT
    TTGAAAACAT ACCTGGAAGT AGATGACATT CCCAACAATC TCTGTGAGCG TCTCTTCATG
    TCTTTTCAAG CAACCCCAAT TGAGAGCAAA AAAAGTGGGG AGGATGAGAC AGAAGGCATA
    AAAGGTAGTG GCCGTATCAG TCTAAATGAT GTGTCCTGTT ATTTCTCTTT GCTGGAAAGT
    GGCACTGCTG AGGATAAACT GGCATTCACC TTTAAATTGT ATGACAAAGA CAGCAATGGT
    CTTCTGGACA GCTCTGAGGT GGACAGGATT ATAACACAAA TGATGCGTGT AGCGGAATAT
    CTGGACTGGG ACATCACGGA ACTCAAACCT ATGTTAGAGG AGATGATGAG ACAAGTGGAC
    TATGATGGAA ATGGAATAGT CTCTTATGAA GAATGGGTTA AAGGAGGACT CACAACAGTG
    CCTCTGCTGG TGCTTCTAGG AATGGAAGTT AATATAAAAG ACGATGGGCA GCACCTGTGG
    CGCCTGAAGC ATTTCAACCG CCCTGTGTAT TGCAATGTGT GTGAAGCTCT TCTTTTTGGG
    GTCAGGAAGC AGGGCCTGTG TTGCACTTTC TGTAAGTTTA CCGTTCATGA ACGCTGTGCC
    CGCAAAGCGC CTAATTCCTG CATAAGCACG TATGCCAAAT CACGCAAGGA TTTCAGCGAG
    CAGGCCCATG TTTGGATGAG AGGTGGATGT GAGGCCAGCA AGTGTGACAA ATGCCAAAAA
    AAAATAAAAA GTTTCCAGAG CCTGACTGGG CAGCGCTGTG TGTGGTGTCA TACAAAGGTT
    CATGATGAAT GTGCCTCATC CCCACTGTGT GATGGTGGAA TCTTAAAGGA CCACATATTA
    CCTCCTGCTT GCATATATCC AGTAATCCTG GAACGACAGA ATAGCCAAAA AAATCTTCTA
    GCTTCCTCAG AAGAAGCATC ACAGACGTTT AGTACCCCAG AAGGACAAGT CCTGCGGATT
    GCCCCATTAC CAGGTACACA CCCTCTACTT GTCTTTGTAA ACCCAAAGAG TGGAGGGAAG
    CAGGGGGAAA GAGTGCTGAG GAAATTCCAG TATCTGCTAA ATCCCAGACA AGTCCATAAC
    TTGATAAAGG GAGGCCCTTC CCCAGGGCTG AATTTTTTCC GGGATGTCCC AGAATATCGT
    ATCTTAGTAT GCGGCGGTGA TGGAACAGTC GGTTGGATCC TGGATGCTAT TGACAAGGCA
    AACCTTCCAT ATAGACCACC TGTAGCTGTG CTGCCCCTCG GTACAGGAAA TGACCTTGCG
    CGATGTCTCA GCTGGGGAGG TGGTTATGAT GGAGAAAATC TAATGAAGTT CTTGAAGGAC
    ATTGAAAAGA GTGTACCCAT TAAAATGGAC AGATGGAACA TTGAGGTTAT ACCTGAAAAC
    CCAGATGAGA AGGGAGACCC AGTTCCTTAC GAGATTATAA ACAACTACTT TTCCATTGGG
    GTGGATGCAT CTATAGCTCA CAGATTCCAT AATATGAGGG AAAAATATCC AGAGAAATTC
    AATAGCAGAA TGAAGAACAA ATTGTGGTAT TTTGAGTTTG CTACATCAGA GACTGTTTTC
    TCTACCTGTA AAAAACTTAA AGAGGTCATT ACAATTGAGT GTTGTGGCTC CCCGTTGAAA
    CTCAGCTCTC TGTCCCTGGA AGGCATCGCA GTTATAAATA TTCCAAGCAT GCATGGGGGT
    TCCAACCTAT GGGGAGAGAC TAAGAAACAC TGGGGAGAAC CTCGCAATGC TTCATGTCAT
    AATGCCCCTG AAGCCATTAC TGACCCTGAT GCCTTAAAGT CATGTGTACA AGACCTCAGT
    GACAAGAGGC TGGAGGTAGT AGGACTTGAG GGTGCTATTG AGATGGGGCA GATTTATACT
    GGACTGAAGA GTGCTGGCAA ACGACTTGCA ACATGTTCTG AACTAACAAT AAGGACCACA
    AAGCCCCTCC CAATGCAAAT TGATGGCGAG CCCTGGATGC AGCCACCCTG CACGATAAAA
    ATCACACATA AGAACCAGGC ACCGATGCTG CGTGCCCCTG CGCCCCGTTC TTCAAGCTTT
    TTCTCCCTGA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813262.1
    CDS269..2503
    Translation

    Target ORF information:

    RefSeq Version XM_012957808.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis diacylglycerol kinase alpha (dgka), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957808.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCGACG AGAACAAGGA GTGGGCAATC CTGAGCCCGG AGGACTTCAC CCAACTGCAG 
    AAGTACATTG ATTACAGCAC CAAGAAGGTT AAAGATGTTC AGCAAGAGTT CTATGGGGAT
    GGTACATCTT GCCCTCACTT GGTTGAAGAC ACAATAGATT TTGAAGCATT CAAATCCTTT
    TTGAAAACAT ACCTGGAAGT AGATGACATT CCCAACAATC TCTGTGAGCG TCTCTTCATG
    TCTTTTCAAG CAACCCCAAT TGAGAGCAAA AAAAGTGGGG AGGATGAGAC AGAAGGCATA
    AAAGGTAGTG GCCGTATCAG TCTAAATGAT GTGTCCTGTT ATTTCTCTTT GCTGGAAAGT
    GGCACTGCTG AGGATAAACT GGCATTCACC TTTAAATTGT ATGACAAAGA CAGCAATGGT
    CTTCTGGACA GCTCTGAGGT GGACAGGATT ATAACACAAA TGATGCGTGT AGCGGAATAT
    CTGGACTGGG ACATCACGGA ACTCAAACCT ATGTTAGAGG AGATGATGAG ACAAGTGGAC
    TATGATGGAA ATGGAATAGT CTCTTATGAA GAATGGGTTA AAGGAGGACT CACAACAGTG
    CCTCTGCTGG TGCTTCTAGG AATGGAAGTT AATATAAAAG ACGATGGGCA GCACCTGTGG
    CGCCTGAAGC ATTTCAACCG CCCTGTGTAT TGCAATGTGT GTGAAGCTCT TCTTTTTGGG
    GTCAGGAAGC AGGGCCTGTG TTGCACTTTC TGTAAGTTTA CCGTTCATGA ACGCTGTGCC
    CGCAAAGCGC CTAATTCCTG CATAAGCACG TATGCCAAAT CACGCAAGGA TTTCAGCGAG
    CAGGCCCATG TTTGGATGAG AGGTGGATGT GAGGCCAGCA AGTGTGACAA ATGCCAAAAA
    AAAATAAAAA GTTTCCAGAG CCTGACTGGG CAGCGCTGTG TGTGGTGTCA TACAAAGGTT
    CATGATGAAT GTGCCTCATC CCCACTGTGT GATGGTGGAA TCTTAAAGGA CCACATATTA
    CCTCCTGCTT GCATATATCC AGTAATCCTG GAACGACAGA ATAGCCAAAA AAATCTTCTA
    GCTTCCTCAG AAGAAGCATC ACAGACGTTT AGTACCCCAG AAGGACAAGT CCTGCGGATT
    GCCCCATTAC CAGGTACACA CCCTCTACTT GTCTTTGTAA ACCCAAAGAG TGGAGGGAAG
    CAGGGGGAAA GAGTGCTGAG GAAATTCCAG TATCTGCTAA ATCCCAGACA AGTCCATAAC
    TTGATAAAGG GAGGCCCTTC CCCAGGGCTG AATTTTTTCC GGGATGTCCC AGAATATCGT
    ATCTTAGTAT GCGGCGGTGA TGGAACAGTC GGTTGGATCC TGGATGCTAT TGACAAGGCA
    AACCTTCCAT ATAGACCACC TGTAGCTGTG CTGCCCCTCG GTACAGGAAA TGACCTTGCG
    CGATGTCTCA GCTGGGGAGG TGGTTATGAT GGAGAAAATC TAATGAAGTT CTTGAAGGAC
    ATTGAAAAGA GTGTACCCAT TAAAATGGAC AGATGGAACA TTGAGGTTAT ACCTGAAAAC
    CCAGATGAGA AGGGAGACCC AGTTCCTTAC GAGATTATAA ACAACTACTT TTCCATTGGG
    GTGGATGCAT CTATAGCTCA CAGATTCCAT AATATGAGGG AAAAATATCC AGAGAAATTC
    AATAGCAGAA TGAAGAACAA ATTGTGGTAT TTTGAGTTTG CTACATCAGA GACTGTTTTC
    TCTACCTGTA AAAAACTTAA AGAGGTCATT ACAATTGAGT GTTGTGGCTC CCCGTTGAAA
    CTCAGCTCTC TGTCCCTGGA AGGCATCGCA GTTATAAATA TTCCAAGCAT GCATGGGGGT
    TCCAACCTAT GGGGAGAGAC TAAGAAACAC TGGGGAGAAC CTCGCAATGC TTCATGTCAT
    AATGCCCCTG AAGCCATTAC TGACCCTGAT GCCTTAAAGT CATGTGTACA AGACCTCAGT
    GACAAGAGGC TGGAGGTAGT AGGACTTGAG GGTGCTATTG AGATGGGGCA GATTTATACT
    GGACTGAAGA GTGCTGGCAA ACGACTTGCA ACATGTTCTG AACTAACAAT AAGGACCACA
    AAGCCCCTCC CAATGCAAAT TGATGGCGAG CCCTGGATGC AGCCACCCTG CACGATAAAA
    ATCACACATA AGAACCAGGC ACCGATGCTG CGTGCCCCTG CGCCCCGTTC TTCAAGCTTT
    TTCTCCCTGA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52684
    Clone ID Related Accession (Same CDS sequence) XM_012957813.3 , XM_012957814.3 , XM_031897165.1 , XM_018091693.2 , XM_012957812.3 , XM_012957808.3 , XM_012957809.3 , XM_012957811.3 , XM_012957810.3
    Accession Version XM_012957809.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2235bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product diacylglycerol kinase alpha
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957809.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCGACG AGAACAAGGA GTGGGCAATC CTGAGCCCGG AGGACTTCAC CCAACTGCAG 
    AAGTACATTG ATTACAGCAC CAAGAAGGTT AAAGATGTTC AGCAAGAGTT CTATGGGGAT
    GGTACATCTT GCCCTCACTT GGTTGAAGAC ACAATAGATT TTGAAGCATT CAAATCCTTT
    TTGAAAACAT ACCTGGAAGT AGATGACATT CCCAACAATC TCTGTGAGCG TCTCTTCATG
    TCTTTTCAAG CAACCCCAAT TGAGAGCAAA AAAAGTGGGG AGGATGAGAC AGAAGGCATA
    AAAGGTAGTG GCCGTATCAG TCTAAATGAT GTGTCCTGTT ATTTCTCTTT GCTGGAAAGT
    GGCACTGCTG AGGATAAACT GGCATTCACC TTTAAATTGT ATGACAAAGA CAGCAATGGT
    CTTCTGGACA GCTCTGAGGT GGACAGGATT ATAACACAAA TGATGCGTGT AGCGGAATAT
    CTGGACTGGG ACATCACGGA ACTCAAACCT ATGTTAGAGG AGATGATGAG ACAAGTGGAC
    TATGATGGAA ATGGAATAGT CTCTTATGAA GAATGGGTTA AAGGAGGACT CACAACAGTG
    CCTCTGCTGG TGCTTCTAGG AATGGAAGTT AATATAAAAG ACGATGGGCA GCACCTGTGG
    CGCCTGAAGC ATTTCAACCG CCCTGTGTAT TGCAATGTGT GTGAAGCTCT TCTTTTTGGG
    GTCAGGAAGC AGGGCCTGTG TTGCACTTTC TGTAAGTTTA CCGTTCATGA ACGCTGTGCC
    CGCAAAGCGC CTAATTCCTG CATAAGCACG TATGCCAAAT CACGCAAGGA TTTCAGCGAG
    CAGGCCCATG TTTGGATGAG AGGTGGATGT GAGGCCAGCA AGTGTGACAA ATGCCAAAAA
    AAAATAAAAA GTTTCCAGAG CCTGACTGGG CAGCGCTGTG TGTGGTGTCA TACAAAGGTT
    CATGATGAAT GTGCCTCATC CCCACTGTGT GATGGTGGAA TCTTAAAGGA CCACATATTA
    CCTCCTGCTT GCATATATCC AGTAATCCTG GAACGACAGA ATAGCCAAAA AAATCTTCTA
    GCTTCCTCAG AAGAAGCATC ACAGACGTTT AGTACCCCAG AAGGACAAGT CCTGCGGATT
    GCCCCATTAC CAGGTACACA CCCTCTACTT GTCTTTGTAA ACCCAAAGAG TGGAGGGAAG
    CAGGGGGAAA GAGTGCTGAG GAAATTCCAG TATCTGCTAA ATCCCAGACA AGTCCATAAC
    TTGATAAAGG GAGGCCCTTC CCCAGGGCTG AATTTTTTCC GGGATGTCCC AGAATATCGT
    ATCTTAGTAT GCGGCGGTGA TGGAACAGTC GGTTGGATCC TGGATGCTAT TGACAAGGCA
    AACCTTCCAT ATAGACCACC TGTAGCTGTG CTGCCCCTCG GTACAGGAAA TGACCTTGCG
    CGATGTCTCA GCTGGGGAGG TGGTTATGAT GGAGAAAATC TAATGAAGTT CTTGAAGGAC
    ATTGAAAAGA GTGTACCCAT TAAAATGGAC AGATGGAACA TTGAGGTTAT ACCTGAAAAC
    CCAGATGAGA AGGGAGACCC AGTTCCTTAC GAGATTATAA ACAACTACTT TTCCATTGGG
    GTGGATGCAT CTATAGCTCA CAGATTCCAT AATATGAGGG AAAAATATCC AGAGAAATTC
    AATAGCAGAA TGAAGAACAA ATTGTGGTAT TTTGAGTTTG CTACATCAGA GACTGTTTTC
    TCTACCTGTA AAAAACTTAA AGAGGTCATT ACAATTGAGT GTTGTGGCTC CCCGTTGAAA
    CTCAGCTCTC TGTCCCTGGA AGGCATCGCA GTTATAAATA TTCCAAGCAT GCATGGGGGT
    TCCAACCTAT GGGGAGAGAC TAAGAAACAC TGGGGAGAAC CTCGCAATGC TTCATGTCAT
    AATGCCCCTG AAGCCATTAC TGACCCTGAT GCCTTAAAGT CATGTGTACA AGACCTCAGT
    GACAAGAGGC TGGAGGTAGT AGGACTTGAG GGTGCTATTG AGATGGGGCA GATTTATACT
    GGACTGAAGA GTGCTGGCAA ACGACTTGCA ACATGTTCTG AACTAACAAT AAGGACCACA
    AAGCCCCTCC CAATGCAAAT TGATGGCGAG CCCTGGATGC AGCCACCCTG CACGATAAAA
    ATCACACATA AGAACCAGGC ACCGATGCTG CGTGCCCCTG CGCCCCGTTC TTCAAGCTTT
    TTCTCCCTGA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813263.1
    CDS295..2529
    Translation

    Target ORF information:

    RefSeq Version XM_012957809.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis diacylglycerol kinase alpha (dgka), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957809.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCGACG AGAACAAGGA GTGGGCAATC CTGAGCCCGG AGGACTTCAC CCAACTGCAG 
    AAGTACATTG ATTACAGCAC CAAGAAGGTT AAAGATGTTC AGCAAGAGTT CTATGGGGAT
    GGTACATCTT GCCCTCACTT GGTTGAAGAC ACAATAGATT TTGAAGCATT CAAATCCTTT
    TTGAAAACAT ACCTGGAAGT AGATGACATT CCCAACAATC TCTGTGAGCG TCTCTTCATG
    TCTTTTCAAG CAACCCCAAT TGAGAGCAAA AAAAGTGGGG AGGATGAGAC AGAAGGCATA
    AAAGGTAGTG GCCGTATCAG TCTAAATGAT GTGTCCTGTT ATTTCTCTTT GCTGGAAAGT
    GGCACTGCTG AGGATAAACT GGCATTCACC TTTAAATTGT ATGACAAAGA CAGCAATGGT
    CTTCTGGACA GCTCTGAGGT GGACAGGATT ATAACACAAA TGATGCGTGT AGCGGAATAT
    CTGGACTGGG ACATCACGGA ACTCAAACCT ATGTTAGAGG AGATGATGAG ACAAGTGGAC
    TATGATGGAA ATGGAATAGT CTCTTATGAA GAATGGGTTA AAGGAGGACT CACAACAGTG
    CCTCTGCTGG TGCTTCTAGG AATGGAAGTT AATATAAAAG ACGATGGGCA GCACCTGTGG
    CGCCTGAAGC ATTTCAACCG CCCTGTGTAT TGCAATGTGT GTGAAGCTCT TCTTTTTGGG
    GTCAGGAAGC AGGGCCTGTG TTGCACTTTC TGTAAGTTTA CCGTTCATGA ACGCTGTGCC
    CGCAAAGCGC CTAATTCCTG CATAAGCACG TATGCCAAAT CACGCAAGGA TTTCAGCGAG
    CAGGCCCATG TTTGGATGAG AGGTGGATGT GAGGCCAGCA AGTGTGACAA ATGCCAAAAA
    AAAATAAAAA GTTTCCAGAG CCTGACTGGG CAGCGCTGTG TGTGGTGTCA TACAAAGGTT
    CATGATGAAT GTGCCTCATC CCCACTGTGT GATGGTGGAA TCTTAAAGGA CCACATATTA
    CCTCCTGCTT GCATATATCC AGTAATCCTG GAACGACAGA ATAGCCAAAA AAATCTTCTA
    GCTTCCTCAG AAGAAGCATC ACAGACGTTT AGTACCCCAG AAGGACAAGT CCTGCGGATT
    GCCCCATTAC CAGGTACACA CCCTCTACTT GTCTTTGTAA ACCCAAAGAG TGGAGGGAAG
    CAGGGGGAAA GAGTGCTGAG GAAATTCCAG TATCTGCTAA ATCCCAGACA AGTCCATAAC
    TTGATAAAGG GAGGCCCTTC CCCAGGGCTG AATTTTTTCC GGGATGTCCC AGAATATCGT
    ATCTTAGTAT GCGGCGGTGA TGGAACAGTC GGTTGGATCC TGGATGCTAT TGACAAGGCA
    AACCTTCCAT ATAGACCACC TGTAGCTGTG CTGCCCCTCG GTACAGGAAA TGACCTTGCG
    CGATGTCTCA GCTGGGGAGG TGGTTATGAT GGAGAAAATC TAATGAAGTT CTTGAAGGAC
    ATTGAAAAGA GTGTACCCAT TAAAATGGAC AGATGGAACA TTGAGGTTAT ACCTGAAAAC
    CCAGATGAGA AGGGAGACCC AGTTCCTTAC GAGATTATAA ACAACTACTT TTCCATTGGG
    GTGGATGCAT CTATAGCTCA CAGATTCCAT AATATGAGGG AAAAATATCC AGAGAAATTC
    AATAGCAGAA TGAAGAACAA ATTGTGGTAT TTTGAGTTTG CTACATCAGA GACTGTTTTC
    TCTACCTGTA AAAAACTTAA AGAGGTCATT ACAATTGAGT GTTGTGGCTC CCCGTTGAAA
    CTCAGCTCTC TGTCCCTGGA AGGCATCGCA GTTATAAATA TTCCAAGCAT GCATGGGGGT
    TCCAACCTAT GGGGAGAGAC TAAGAAACAC TGGGGAGAAC CTCGCAATGC TTCATGTCAT
    AATGCCCCTG AAGCCATTAC TGACCCTGAT GCCTTAAAGT CATGTGTACA AGACCTCAGT
    GACAAGAGGC TGGAGGTAGT AGGACTTGAG GGTGCTATTG AGATGGGGCA GATTTATACT
    GGACTGAAGA GTGCTGGCAA ACGACTTGCA ACATGTTCTG AACTAACAAT AAGGACCACA
    AAGCCCCTCC CAATGCAAAT TGATGGCGAG CCCTGGATGC AGCCACCCTG CACGATAAAA
    ATCACACATA AGAACCAGGC ACCGATGCTG CGTGCCCCTG CGCCCCGTTC TTCAAGCTTT
    TTCTCCCTGA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52684
    Clone ID Related Accession (Same CDS sequence) XM_012957813.3 , XM_012957814.3 , XM_031897165.1 , XM_018091693.2 , XM_012957812.3 , XM_012957808.3 , XM_012957809.3 , XM_012957811.3 , XM_012957810.3
    Accession Version XM_012957811.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2235bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product diacylglycerol kinase alpha
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957811.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCGACG AGAACAAGGA GTGGGCAATC CTGAGCCCGG AGGACTTCAC CCAACTGCAG 
    AAGTACATTG ATTACAGCAC CAAGAAGGTT AAAGATGTTC AGCAAGAGTT CTATGGGGAT
    GGTACATCTT GCCCTCACTT GGTTGAAGAC ACAATAGATT TTGAAGCATT CAAATCCTTT
    TTGAAAACAT ACCTGGAAGT AGATGACATT CCCAACAATC TCTGTGAGCG TCTCTTCATG
    TCTTTTCAAG CAACCCCAAT TGAGAGCAAA AAAAGTGGGG AGGATGAGAC AGAAGGCATA
    AAAGGTAGTG GCCGTATCAG TCTAAATGAT GTGTCCTGTT ATTTCTCTTT GCTGGAAAGT
    GGCACTGCTG AGGATAAACT GGCATTCACC TTTAAATTGT ATGACAAAGA CAGCAATGGT
    CTTCTGGACA GCTCTGAGGT GGACAGGATT ATAACACAAA TGATGCGTGT AGCGGAATAT
    CTGGACTGGG ACATCACGGA ACTCAAACCT ATGTTAGAGG AGATGATGAG ACAAGTGGAC
    TATGATGGAA ATGGAATAGT CTCTTATGAA GAATGGGTTA AAGGAGGACT CACAACAGTG
    CCTCTGCTGG TGCTTCTAGG AATGGAAGTT AATATAAAAG ACGATGGGCA GCACCTGTGG
    CGCCTGAAGC ATTTCAACCG CCCTGTGTAT TGCAATGTGT GTGAAGCTCT TCTTTTTGGG
    GTCAGGAAGC AGGGCCTGTG TTGCACTTTC TGTAAGTTTA CCGTTCATGA ACGCTGTGCC
    CGCAAAGCGC CTAATTCCTG CATAAGCACG TATGCCAAAT CACGCAAGGA TTTCAGCGAG
    CAGGCCCATG TTTGGATGAG AGGTGGATGT GAGGCCAGCA AGTGTGACAA ATGCCAAAAA
    AAAATAAAAA GTTTCCAGAG CCTGACTGGG CAGCGCTGTG TGTGGTGTCA TACAAAGGTT
    CATGATGAAT GTGCCTCATC CCCACTGTGT GATGGTGGAA TCTTAAAGGA CCACATATTA
    CCTCCTGCTT GCATATATCC AGTAATCCTG GAACGACAGA ATAGCCAAAA AAATCTTCTA
    GCTTCCTCAG AAGAAGCATC ACAGACGTTT AGTACCCCAG AAGGACAAGT CCTGCGGATT
    GCCCCATTAC CAGGTACACA CCCTCTACTT GTCTTTGTAA ACCCAAAGAG TGGAGGGAAG
    CAGGGGGAAA GAGTGCTGAG GAAATTCCAG TATCTGCTAA ATCCCAGACA AGTCCATAAC
    TTGATAAAGG GAGGCCCTTC CCCAGGGCTG AATTTTTTCC GGGATGTCCC AGAATATCGT
    ATCTTAGTAT GCGGCGGTGA TGGAACAGTC GGTTGGATCC TGGATGCTAT TGACAAGGCA
    AACCTTCCAT ATAGACCACC TGTAGCTGTG CTGCCCCTCG GTACAGGAAA TGACCTTGCG
    CGATGTCTCA GCTGGGGAGG TGGTTATGAT GGAGAAAATC TAATGAAGTT CTTGAAGGAC
    ATTGAAAAGA GTGTACCCAT TAAAATGGAC AGATGGAACA TTGAGGTTAT ACCTGAAAAC
    CCAGATGAGA AGGGAGACCC AGTTCCTTAC GAGATTATAA ACAACTACTT TTCCATTGGG
    GTGGATGCAT CTATAGCTCA CAGATTCCAT AATATGAGGG AAAAATATCC AGAGAAATTC
    AATAGCAGAA TGAAGAACAA ATTGTGGTAT TTTGAGTTTG CTACATCAGA GACTGTTTTC
    TCTACCTGTA AAAAACTTAA AGAGGTCATT ACAATTGAGT GTTGTGGCTC CCCGTTGAAA
    CTCAGCTCTC TGTCCCTGGA AGGCATCGCA GTTATAAATA TTCCAAGCAT GCATGGGGGT
    TCCAACCTAT GGGGAGAGAC TAAGAAACAC TGGGGAGAAC CTCGCAATGC TTCATGTCAT
    AATGCCCCTG AAGCCATTAC TGACCCTGAT GCCTTAAAGT CATGTGTACA AGACCTCAGT
    GACAAGAGGC TGGAGGTAGT AGGACTTGAG GGTGCTATTG AGATGGGGCA GATTTATACT
    GGACTGAAGA GTGCTGGCAA ACGACTTGCA ACATGTTCTG AACTAACAAT AAGGACCACA
    AAGCCCCTCC CAATGCAAAT TGATGGCGAG CCCTGGATGC AGCCACCCTG CACGATAAAA
    ATCACACATA AGAACCAGGC ACCGATGCTG CGTGCCCCTG CGCCCCGTTC TTCAAGCTTT
    TTCTCCCTGA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813265.1
    CDS406..2640
    Translation

    Target ORF information:

    RefSeq Version XM_012957811.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis diacylglycerol kinase alpha (dgka), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957811.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCGACG AGAACAAGGA GTGGGCAATC CTGAGCCCGG AGGACTTCAC CCAACTGCAG 
    AAGTACATTG ATTACAGCAC CAAGAAGGTT AAAGATGTTC AGCAAGAGTT CTATGGGGAT
    GGTACATCTT GCCCTCACTT GGTTGAAGAC ACAATAGATT TTGAAGCATT CAAATCCTTT
    TTGAAAACAT ACCTGGAAGT AGATGACATT CCCAACAATC TCTGTGAGCG TCTCTTCATG
    TCTTTTCAAG CAACCCCAAT TGAGAGCAAA AAAAGTGGGG AGGATGAGAC AGAAGGCATA
    AAAGGTAGTG GCCGTATCAG TCTAAATGAT GTGTCCTGTT ATTTCTCTTT GCTGGAAAGT
    GGCACTGCTG AGGATAAACT GGCATTCACC TTTAAATTGT ATGACAAAGA CAGCAATGGT
    CTTCTGGACA GCTCTGAGGT GGACAGGATT ATAACACAAA TGATGCGTGT AGCGGAATAT
    CTGGACTGGG ACATCACGGA ACTCAAACCT ATGTTAGAGG AGATGATGAG ACAAGTGGAC
    TATGATGGAA ATGGAATAGT CTCTTATGAA GAATGGGTTA AAGGAGGACT CACAACAGTG
    CCTCTGCTGG TGCTTCTAGG AATGGAAGTT AATATAAAAG ACGATGGGCA GCACCTGTGG
    CGCCTGAAGC ATTTCAACCG CCCTGTGTAT TGCAATGTGT GTGAAGCTCT TCTTTTTGGG
    GTCAGGAAGC AGGGCCTGTG TTGCACTTTC TGTAAGTTTA CCGTTCATGA ACGCTGTGCC
    CGCAAAGCGC CTAATTCCTG CATAAGCACG TATGCCAAAT CACGCAAGGA TTTCAGCGAG
    CAGGCCCATG TTTGGATGAG AGGTGGATGT GAGGCCAGCA AGTGTGACAA ATGCCAAAAA
    AAAATAAAAA GTTTCCAGAG CCTGACTGGG CAGCGCTGTG TGTGGTGTCA TACAAAGGTT
    CATGATGAAT GTGCCTCATC CCCACTGTGT GATGGTGGAA TCTTAAAGGA CCACATATTA
    CCTCCTGCTT GCATATATCC AGTAATCCTG GAACGACAGA ATAGCCAAAA AAATCTTCTA
    GCTTCCTCAG AAGAAGCATC ACAGACGTTT AGTACCCCAG AAGGACAAGT CCTGCGGATT
    GCCCCATTAC CAGGTACACA CCCTCTACTT GTCTTTGTAA ACCCAAAGAG TGGAGGGAAG
    CAGGGGGAAA GAGTGCTGAG GAAATTCCAG TATCTGCTAA ATCCCAGACA AGTCCATAAC
    TTGATAAAGG GAGGCCCTTC CCCAGGGCTG AATTTTTTCC GGGATGTCCC AGAATATCGT
    ATCTTAGTAT GCGGCGGTGA TGGAACAGTC GGTTGGATCC TGGATGCTAT TGACAAGGCA
    AACCTTCCAT ATAGACCACC TGTAGCTGTG CTGCCCCTCG GTACAGGAAA TGACCTTGCG
    CGATGTCTCA GCTGGGGAGG TGGTTATGAT GGAGAAAATC TAATGAAGTT CTTGAAGGAC
    ATTGAAAAGA GTGTACCCAT TAAAATGGAC AGATGGAACA TTGAGGTTAT ACCTGAAAAC
    CCAGATGAGA AGGGAGACCC AGTTCCTTAC GAGATTATAA ACAACTACTT TTCCATTGGG
    GTGGATGCAT CTATAGCTCA CAGATTCCAT AATATGAGGG AAAAATATCC AGAGAAATTC
    AATAGCAGAA TGAAGAACAA ATTGTGGTAT TTTGAGTTTG CTACATCAGA GACTGTTTTC
    TCTACCTGTA AAAAACTTAA AGAGGTCATT ACAATTGAGT GTTGTGGCTC CCCGTTGAAA
    CTCAGCTCTC TGTCCCTGGA AGGCATCGCA GTTATAAATA TTCCAAGCAT GCATGGGGGT
    TCCAACCTAT GGGGAGAGAC TAAGAAACAC TGGGGAGAAC CTCGCAATGC TTCATGTCAT
    AATGCCCCTG AAGCCATTAC TGACCCTGAT GCCTTAAAGT CATGTGTACA AGACCTCAGT
    GACAAGAGGC TGGAGGTAGT AGGACTTGAG GGTGCTATTG AGATGGGGCA GATTTATACT
    GGACTGAAGA GTGCTGGCAA ACGACTTGCA ACATGTTCTG AACTAACAAT AAGGACCACA
    AAGCCCCTCC CAATGCAAAT TGATGGCGAG CCCTGGATGC AGCCACCCTG CACGATAAAA
    ATCACACATA AGAACCAGGC ACCGATGCTG CGTGCCCCTG CGCCCCGTTC TTCAAGCTTT
    TTCTCCCTGA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52684
    Clone ID Related Accession (Same CDS sequence) XM_012957813.3 , XM_012957814.3 , XM_031897165.1 , XM_018091693.2 , XM_012957812.3 , XM_012957808.3 , XM_012957809.3 , XM_012957811.3 , XM_012957810.3
    Accession Version XM_012957810.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2235bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product diacylglycerol kinase alpha
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957810.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCGACG AGAACAAGGA GTGGGCAATC CTGAGCCCGG AGGACTTCAC CCAACTGCAG 
    AAGTACATTG ATTACAGCAC CAAGAAGGTT AAAGATGTTC AGCAAGAGTT CTATGGGGAT
    GGTACATCTT GCCCTCACTT GGTTGAAGAC ACAATAGATT TTGAAGCATT CAAATCCTTT
    TTGAAAACAT ACCTGGAAGT AGATGACATT CCCAACAATC TCTGTGAGCG TCTCTTCATG
    TCTTTTCAAG CAACCCCAAT TGAGAGCAAA AAAAGTGGGG AGGATGAGAC AGAAGGCATA
    AAAGGTAGTG GCCGTATCAG TCTAAATGAT GTGTCCTGTT ATTTCTCTTT GCTGGAAAGT
    GGCACTGCTG AGGATAAACT GGCATTCACC TTTAAATTGT ATGACAAAGA CAGCAATGGT
    CTTCTGGACA GCTCTGAGGT GGACAGGATT ATAACACAAA TGATGCGTGT AGCGGAATAT
    CTGGACTGGG ACATCACGGA ACTCAAACCT ATGTTAGAGG AGATGATGAG ACAAGTGGAC
    TATGATGGAA ATGGAATAGT CTCTTATGAA GAATGGGTTA AAGGAGGACT CACAACAGTG
    CCTCTGCTGG TGCTTCTAGG AATGGAAGTT AATATAAAAG ACGATGGGCA GCACCTGTGG
    CGCCTGAAGC ATTTCAACCG CCCTGTGTAT TGCAATGTGT GTGAAGCTCT TCTTTTTGGG
    GTCAGGAAGC AGGGCCTGTG TTGCACTTTC TGTAAGTTTA CCGTTCATGA ACGCTGTGCC
    CGCAAAGCGC CTAATTCCTG CATAAGCACG TATGCCAAAT CACGCAAGGA TTTCAGCGAG
    CAGGCCCATG TTTGGATGAG AGGTGGATGT GAGGCCAGCA AGTGTGACAA ATGCCAAAAA
    AAAATAAAAA GTTTCCAGAG CCTGACTGGG CAGCGCTGTG TGTGGTGTCA TACAAAGGTT
    CATGATGAAT GTGCCTCATC CCCACTGTGT GATGGTGGAA TCTTAAAGGA CCACATATTA
    CCTCCTGCTT GCATATATCC AGTAATCCTG GAACGACAGA ATAGCCAAAA AAATCTTCTA
    GCTTCCTCAG AAGAAGCATC ACAGACGTTT AGTACCCCAG AAGGACAAGT CCTGCGGATT
    GCCCCATTAC CAGGTACACA CCCTCTACTT GTCTTTGTAA ACCCAAAGAG TGGAGGGAAG
    CAGGGGGAAA GAGTGCTGAG GAAATTCCAG TATCTGCTAA ATCCCAGACA AGTCCATAAC
    TTGATAAAGG GAGGCCCTTC CCCAGGGCTG AATTTTTTCC GGGATGTCCC AGAATATCGT
    ATCTTAGTAT GCGGCGGTGA TGGAACAGTC GGTTGGATCC TGGATGCTAT TGACAAGGCA
    AACCTTCCAT ATAGACCACC TGTAGCTGTG CTGCCCCTCG GTACAGGAAA TGACCTTGCG
    CGATGTCTCA GCTGGGGAGG TGGTTATGAT GGAGAAAATC TAATGAAGTT CTTGAAGGAC
    ATTGAAAAGA GTGTACCCAT TAAAATGGAC AGATGGAACA TTGAGGTTAT ACCTGAAAAC
    CCAGATGAGA AGGGAGACCC AGTTCCTTAC GAGATTATAA ACAACTACTT TTCCATTGGG
    GTGGATGCAT CTATAGCTCA CAGATTCCAT AATATGAGGG AAAAATATCC AGAGAAATTC
    AATAGCAGAA TGAAGAACAA ATTGTGGTAT TTTGAGTTTG CTACATCAGA GACTGTTTTC
    TCTACCTGTA AAAAACTTAA AGAGGTCATT ACAATTGAGT GTTGTGGCTC CCCGTTGAAA
    CTCAGCTCTC TGTCCCTGGA AGGCATCGCA GTTATAAATA TTCCAAGCAT GCATGGGGGT
    TCCAACCTAT GGGGAGAGAC TAAGAAACAC TGGGGAGAAC CTCGCAATGC TTCATGTCAT
    AATGCCCCTG AAGCCATTAC TGACCCTGAT GCCTTAAAGT CATGTGTACA AGACCTCAGT
    GACAAGAGGC TGGAGGTAGT AGGACTTGAG GGTGCTATTG AGATGGGGCA GATTTATACT
    GGACTGAAGA GTGCTGGCAA ACGACTTGCA ACATGTTCTG AACTAACAAT AAGGACCACA
    AAGCCCCTCC CAATGCAAAT TGATGGCGAG CCCTGGATGC AGCCACCCTG CACGATAAAA
    ATCACACATA AGAACCAGGC ACCGATGCTG CGTGCCCCTG CGCCCCGTTC TTCAAGCTTT
    TTCTCCCTGA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813264.1
    CDS188..2422
    Translation

    Target ORF information:

    RefSeq Version XM_012957810.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis diacylglycerol kinase alpha (dgka), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957810.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCGACG AGAACAAGGA GTGGGCAATC CTGAGCCCGG AGGACTTCAC CCAACTGCAG 
    AAGTACATTG ATTACAGCAC CAAGAAGGTT AAAGATGTTC AGCAAGAGTT CTATGGGGAT
    GGTACATCTT GCCCTCACTT GGTTGAAGAC ACAATAGATT TTGAAGCATT CAAATCCTTT
    TTGAAAACAT ACCTGGAAGT AGATGACATT CCCAACAATC TCTGTGAGCG TCTCTTCATG
    TCTTTTCAAG CAACCCCAAT TGAGAGCAAA AAAAGTGGGG AGGATGAGAC AGAAGGCATA
    AAAGGTAGTG GCCGTATCAG TCTAAATGAT GTGTCCTGTT ATTTCTCTTT GCTGGAAAGT
    GGCACTGCTG AGGATAAACT GGCATTCACC TTTAAATTGT ATGACAAAGA CAGCAATGGT
    CTTCTGGACA GCTCTGAGGT GGACAGGATT ATAACACAAA TGATGCGTGT AGCGGAATAT
    CTGGACTGGG ACATCACGGA ACTCAAACCT ATGTTAGAGG AGATGATGAG ACAAGTGGAC
    TATGATGGAA ATGGAATAGT CTCTTATGAA GAATGGGTTA AAGGAGGACT CACAACAGTG
    CCTCTGCTGG TGCTTCTAGG AATGGAAGTT AATATAAAAG ACGATGGGCA GCACCTGTGG
    CGCCTGAAGC ATTTCAACCG CCCTGTGTAT TGCAATGTGT GTGAAGCTCT TCTTTTTGGG
    GTCAGGAAGC AGGGCCTGTG TTGCACTTTC TGTAAGTTTA CCGTTCATGA ACGCTGTGCC
    CGCAAAGCGC CTAATTCCTG CATAAGCACG TATGCCAAAT CACGCAAGGA TTTCAGCGAG
    CAGGCCCATG TTTGGATGAG AGGTGGATGT GAGGCCAGCA AGTGTGACAA ATGCCAAAAA
    AAAATAAAAA GTTTCCAGAG CCTGACTGGG CAGCGCTGTG TGTGGTGTCA TACAAAGGTT
    CATGATGAAT GTGCCTCATC CCCACTGTGT GATGGTGGAA TCTTAAAGGA CCACATATTA
    CCTCCTGCTT GCATATATCC AGTAATCCTG GAACGACAGA ATAGCCAAAA AAATCTTCTA
    GCTTCCTCAG AAGAAGCATC ACAGACGTTT AGTACCCCAG AAGGACAAGT CCTGCGGATT
    GCCCCATTAC CAGGTACACA CCCTCTACTT GTCTTTGTAA ACCCAAAGAG TGGAGGGAAG
    CAGGGGGAAA GAGTGCTGAG GAAATTCCAG TATCTGCTAA ATCCCAGACA AGTCCATAAC
    TTGATAAAGG GAGGCCCTTC CCCAGGGCTG AATTTTTTCC GGGATGTCCC AGAATATCGT
    ATCTTAGTAT GCGGCGGTGA TGGAACAGTC GGTTGGATCC TGGATGCTAT TGACAAGGCA
    AACCTTCCAT ATAGACCACC TGTAGCTGTG CTGCCCCTCG GTACAGGAAA TGACCTTGCG
    CGATGTCTCA GCTGGGGAGG TGGTTATGAT GGAGAAAATC TAATGAAGTT CTTGAAGGAC
    ATTGAAAAGA GTGTACCCAT TAAAATGGAC AGATGGAACA TTGAGGTTAT ACCTGAAAAC
    CCAGATGAGA AGGGAGACCC AGTTCCTTAC GAGATTATAA ACAACTACTT TTCCATTGGG
    GTGGATGCAT CTATAGCTCA CAGATTCCAT AATATGAGGG AAAAATATCC AGAGAAATTC
    AATAGCAGAA TGAAGAACAA ATTGTGGTAT TTTGAGTTTG CTACATCAGA GACTGTTTTC
    TCTACCTGTA AAAAACTTAA AGAGGTCATT ACAATTGAGT GTTGTGGCTC CCCGTTGAAA
    CTCAGCTCTC TGTCCCTGGA AGGCATCGCA GTTATAAATA TTCCAAGCAT GCATGGGGGT
    TCCAACCTAT GGGGAGAGAC TAAGAAACAC TGGGGAGAAC CTCGCAATGC TTCATGTCAT
    AATGCCCCTG AAGCCATTAC TGACCCTGAT GCCTTAAAGT CATGTGTACA AGACCTCAGT
    GACAAGAGGC TGGAGGTAGT AGGACTTGAG GGTGCTATTG AGATGGGGCA GATTTATACT
    GGACTGAAGA GTGCTGGCAA ACGACTTGCA ACATGTTCTG AACTAACAAT AAGGACCACA
    AAGCCCCTCC CAATGCAAAT TGATGGCGAG CCCTGGATGC AGCCACCCTG CACGATAAAA
    ATCACACATA AGAACCAGGC ACCGATGCTG CGTGCCCCTG CGCCCCGTTC TTCAAGCTTT
    TTCTCCCTGA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.