cadps cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following cadps gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cadps cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa43698 XM_018092881.1
Latest version!
Xenopus tropicalis Ca++-dependent secretion activator (cadps), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OXa43699 XM_018092882.1
Latest version!
Xenopus tropicalis Ca++-dependent secretion activator (cadps), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OXa43700 XM_018092883.1
Latest version!
Xenopus tropicalis Ca++-dependent secretion activator (cadps), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OXa43701 XM_018092884.1
Latest version!
Xenopus tropicalis Ca++-dependent secretion activator (cadps), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OXa43702 XM_018092885.1
Latest version!
Xenopus tropicalis Ca++-dependent secretion activator (cadps), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OXa43703 XM_018092886.1
Latest version!
Xenopus tropicalis Ca++-dependent secretion activator (cadps), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OXa43704 XM_018092887.1
Latest version!
Xenopus tropicalis Ca++-dependent secretion activator (cadps), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OXa55636 XM_012960425.3
Latest version!
Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa55637 XM_018092881.2
Latest version!
Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa55638 XM_018092882.2
Latest version!
Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa55639 XM_012960426.3
Latest version!
Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa55640 XM_018092883.2
Latest version!
Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa55641 XM_018092884.2
Latest version!
Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa55642 XM_018092885.2
Latest version!
Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa55643 XM_012960428.3
Latest version!
Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa55644 XM_018092886.2
Latest version!
Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa55645 XM_012960429.3
Latest version!
Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa55646 XM_012960430.3
Latest version!
Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa55647 XM_012960431.3
Latest version!
Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa55648 XM_012960432.3
Latest version!
Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa55649 XM_012960440.3
Latest version!
Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa55650 XM_018092887.2
Latest version!
Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa55651 XM_012960433.3
Latest version!
Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa55652 XM_012960434.3
Latest version!
Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa55653 XM_031899728.1
Latest version!
Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa55654 XM_031899729.1
Latest version!
Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa55655 XM_012960435.3
Latest version!
Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa55656 XM_012960436.3
Latest version!
Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa55657 XM_012960437.3
Latest version!
Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa55658 XM_031899730.1
Latest version!
Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa55659 XM_012960442.3
Latest version!
Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa55660 XM_012960438.3
Latest version!
Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X25, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa55661 XM_012960441.3
Latest version!
Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X26, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa23892 NM_001102712.1
Latest version!
Xenopus tropicalis Ca2+ dependent secretion activator (cadps), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa30366 XM_012960429.2
Latest version!
Xenopus tropicalis Ca++-dependent secretion activator (cadps), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa30367 XM_012960430.2
Latest version!
Xenopus tropicalis Ca++-dependent secretion activator (cadps), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa30368 XM_012960431.2
Latest version!
Xenopus tropicalis Ca++-dependent secretion activator (cadps), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa30369 XM_012960432.2
Latest version!
Xenopus tropicalis Ca++-dependent secretion activator (cadps), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa30370 XM_012960433.2
Latest version!
Xenopus tropicalis Ca++-dependent secretion activator (cadps), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa30371 XM_012960434.2
Latest version!
Xenopus tropicalis Ca++-dependent secretion activator (cadps), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa30372 XM_012960435.2
Latest version!
Xenopus tropicalis Ca++-dependent secretion activator (cadps), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa30373 XM_012960436.2
Latest version!
Xenopus tropicalis Ca++-dependent secretion activator (cadps), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa30374 XM_012960437.2
Latest version!
Xenopus tropicalis Ca++-dependent secretion activator (cadps), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa30375 XM_012960438.2
Latest version!
Xenopus tropicalis Ca++-dependent secretion activator (cadps), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa30376 XM_012960440.2
Latest version!
Xenopus tropicalis Ca++-dependent secretion activator (cadps), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa30377 XM_012960441.2
Latest version!
Xenopus tropicalis Ca++-dependent secretion activator (cadps), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa30378 XM_012960442.2
Latest version!
Xenopus tropicalis Ca++-dependent secretion activator (cadps), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa30362 XM_012960425.2
Latest version!
Xenopus tropicalis Ca++-dependent secretion activator (cadps), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa30363 XM_012960426.2
Latest version!
Xenopus tropicalis Ca++-dependent secretion activator (cadps), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa30365 XM_012960428.2
Latest version!
Xenopus tropicalis Ca++-dependent secretion activator (cadps), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa43698
    Clone ID Related Accession (Same CDS sequence) XM_018092881.1
    Accession Version XM_018092881.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4062bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGGGAAAA AGAGAGAAAT GTATGAACAT
    CCTGTCTTCT GCTTGGCCTC CCAAGTGATG GATTTAACCA TTCAGAATCA AAAGGACGCA
    GAAAATGTAG GCCGATTAGT AACGCCTGCC AAAAAGCTCG AAGATACAAT ACGGCTCGCA
    GAACTTGTCA TTGAAGTTCT ACAGCAGAAT GAGGAGCACC ATGCGGAGGC GTTTGCTTGG
    TGGTCGGACT TGATGGTGGA ACACGCAGAG ACCTTCCTTT CGCTTTTCGC GGTGGATATG
    GATGCTGCTT TAGAGGTGCA ACCGCCAGAC AGCTGGGACA GCTTCCCTCT TTTTCAGCTA
    ATAAATGACT TCCTTCGAAG TGACTATAAT TTATGCAATG GGAAATTCCA CAAACACCTT
    CAAGATCTCT TTGCTCCACT CGTTGTGAGA TACGTCGACC TCATGGAGTC CTCAATTGCA
    CAGTCAATTC ACAGGGGCTT TGAGCGTGAA TCATGGGAAC CAGTAAAGAG TTTAACAAGT
    AATCTACCCA ATGTGAATCT ACCAAATGTG AATCTCCCTA AAGTACCAGT TACCTTACCA
    GTTAACCTCC CACAAATGCC TAGTTTTTCT GCACCGTCAT GGATGGCAGC TATATATGAT
    TCGGACAATG GATCAGCAAC TTCAGAAGAT CTATTCTGGA AGCTTGACGC TCTGCAAACG
    TTCATTCGAG ATCTTCATTG GCCCGAGGAG GAATTCGGGA AACACCTAGA GCAACGCCTT
    AAACTTATGG CTAGCGATAT GATCGAATCG TGTGTCAAAC GAACTAGAAT TGCATTCGAA
    GTCAAACTGC AGAAAACCAG TCGATCAACA GACTTCCGAG TACCGCAATC AATATGCACC
    ATGTTTAATG TTATGGTTGA TGCCAAAGCT CAGTCAACGA AACTTTGTAG CATGGAAATG
    GGCCAAGAGC ACCAGTACCA CTCCCAAATT GACGAGCTAA TTGAAGAAAC AGTTAAAGAG
    ATGATCACAC TCTTAGTGGC AAAGTTTGTC ACAATTCTAG AAGGAGTTCT GTCCAAACTA
    TCCAGATACG ATGAAGGGAC ATTATTTTCC TCCTTTCTGT CATTTACTGT GAAAGCAGCT
    TCCAAGTATG TGGATGTACC AAAACCCGGG ATGGACCTTG CCGACGCCTA CGTGACATTT
    GTGCGCCACT CTCAGGATGT CCTGCGTGAT AAGGTCAATG AGGAGATATA TATAGAAAGG
    TTATTTGATA AAAGGCTGGA TGGTAATAGT TCTGTCATGT ACCTGAGAAT ATTGGAGCAA
    TGGTATACCA GCTCAATGAA TGTTGTTTGT ACTTGGCTGA CCGACCGTAT GGATCTGCAA
    CTTCACATTT ATCAGCTGAA AACCCTAATT AGGGTCGTCA AGAAAACCTA CCGCGATTTC
    CGATTGCAAG GCGTCTTGGA CTCCACCTTG AACAGCAAGA CCTACGACAC CATTAGAAAC
    CGCCTCACCG TGGAAGAAGC CACAGCGTCC GTGAGCGAGG GGGGAGGCCT CCAGGGAATC
    ACCATGAAGG ACAGCGACGA GGAGGATGAG GAGGATGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017948370.1
    CDS748..4809
    Translation

    Target ORF information:

    RefSeq Version XM_018092881.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca++-dependent secretion activator (cadps), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092881.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGGGAAAA AGAGAGAAAT GTATGAACAT
    CCTGTCTTCT GCTTGGCCTC CCAAGTGATG GATTTAACCA TTCAGAATCA AAAGGACGCA
    GAAAATGTAG GCCGATTAGT AACGCCTGCC AAAAAGCTCG AAGATACAAT ACGGCTCGCA
    GAACTTGTCA TTGAAGTTCT ACAGCAGAAT GAGGAGCACC ATGCGGAGGC GTTTGCTTGG
    TGGTCGGACT TGATGGTGGA ACACGCAGAG ACCTTCCTTT CGCTTTTCGC GGTGGATATG
    GATGCTGCTT TAGAGGTGCA ACCGCCAGAC AGCTGGGACA GCTTCCCTCT TTTTCAGCTA
    ATAAATGACT TCCTTCGAAG TGACTATAAT TTATGCAATG GGAAATTCCA CAAACACCTT
    CAAGATCTCT TTGCTCCACT CGTTGTGAGA TACGTCGACC TCATGGAGTC CTCAATTGCA
    CAGTCAATTC ACAGGGGCTT TGAGCGTGAA TCATGGGAAC CAGTAAAGAG TTTAACAAGT
    AATCTACCCA ATGTGAATCT ACCAAATGTG AATCTCCCTA AAGTACCAGT TACCTTACCA
    GTTAACCTCC CACAAATGCC TAGTTTTTCT GCACCGTCAT GGATGGCAGC TATATATGAT
    TCGGACAATG GATCAGCAAC TTCAGAAGAT CTATTCTGGA AGCTTGACGC TCTGCAAACG
    TTCATTCGAG ATCTTCATTG GCCCGAGGAG GAATTCGGGA AACACCTAGA GCAACGCCTT
    AAACTTATGG CTAGCGATAT GATCGAATCG TGTGTCAAAC GAACTAGAAT TGCATTCGAA
    GTCAAACTGC AGAAAACCAG TCGATCAACA GACTTCCGAG TACCGCAATC AATATGCACC
    ATGTTTAATG TTATGGTTGA TGCCAAAGCT CAGTCAACGA AACTTTGTAG CATGGAAATG
    GGCCAAGAGC ACCAGTACCA CTCCCAAATT GACGAGCTAA TTGAAGAAAC AGTTAAAGAG
    ATGATCACAC TCTTAGTGGC AAAGTTTGTC ACAATTCTAG AAGGAGTTCT GTCCAAACTA
    TCCAGATACG ATGAAGGGAC ATTATTTTCC TCCTTTCTGT CATTTACTGT GAAAGCAGCT
    TCCAAGTATG TGGATGTACC AAAACCCGGG ATGGACCTTG CCGACGCCTA CGTGACATTT
    GTGCGCCACT CTCAGGATGT CCTGCGTGAT AAGGTCAATG AGGAGATATA TATAGAAAGG
    TTATTTGATA AAAGGCTGGA TGGTAATAGT TCTGTCATGT ACCTGAGAAT ATTGGAGCAA
    TGGTATACCA GCTCAATGAA TGTTGTTTGT ACTTGGCTGA CCGACCGTAT GGATCTGCAA
    CTTCACATTT ATCAGCTGAA AACCCTAATT AGGGTCGTCA AGAAAACCTA CCGCGATTTC
    CGATTGCAAG GCGTCTTGGA CTCCACCTTG AACAGCAAGA CCTACGACAC CATTAGAAAC
    CGCCTCACCG TGGAAGAAGC CACAGCGTCC GTGAGCGAGG GGGGAGGCCT CCAGGGAATC
    ACCATGAAGG ACAGCGACGA GGAGGATGAG GAGGATGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa43699
    Clone ID Related Accession (Same CDS sequence) XM_018092882.1
    Accession Version XM_018092882.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4059bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTATG CTGATAGGGC ACAAAAACAT GGCATGGATG AATTTATCTC GTCCAATCCC
    TGTAACTTTG ACCACGCCAC CCTCTTTGAG ATGGTTCAGC GACTCACACT GGATCACAGA
    CTCAACGACT CGTACTCATG TCTGGGTTGG TTCAGTCCAG GTCAGGTCTT TGTACTCGAT
    GAATATTGTG CAAGATATGG AGTCCGAGGC TGCCATAGGC ATTTGTGTTA CCTTGGTGAT
    TTACTAGAAC GAGCAGAGAA TGGGTCAATG GTCGACCCAA CTTTACTTCA CTATAGTTTT
    GCCTTCTGCG CATCCCACGT ACATGGCAAC AGGCCTGATG GCATCGGAAC TGTAACCGTG
    GAAGAAAAAG AGCGCTTTGA AGAGATCAAA GAACGTCTCC GCTTGCTTTT GGAGAATCAG
    ATCACGCATT TTAGGTACTG CTTTCCATTT GGCCGACCAG AAGGTGCTTT GAAAGCCACT
    CTGTCATTGC TGGAAAGGGT TTTAATGAAA GACATAGTAA CTCCCGTCCC TCAAGAAGAA
    GTGAAAAACG TTATCCGTAA ATGCTTAGAG CAAGCAGCCT TAGTTAACTA CACGAGATTA
    TCCGAATATG CCAAAATCGA AGGGAAAAAG AGAGAAATGT ATGAACATCC TGTCTTCTGC
    TTGGCCTCCC AAGTGATGGA TTTAACCATT CAGAATCAAA AGGACGCAGA AAATGTAGGC
    CGATTAGTAA CGCCTGCCAA AAAGCTCGAA GATACAATAC GGCTCGCAGA ACTTGTCATT
    GAAGTTCTAC AGCAGAATGA GGAGCACCAT GCGGAGGGGA AAGAGGCGTT TGCTTGGTGG
    TCGGACTTGA TGGTGGAACA CGCAGAGACC TTCCTTTCGC TTTTCGCGGT GGATATGGAT
    GCTGCTTTAG AGGTGCAACC GCCAGACAGC TGGGACAGCT TCCCTCTTTT TCAGCTAATA
    AATGACTTCC TTCGAAGTGA CTATAATTTA TGCAATGGGA AATTCCACAA ACACCTTCAA
    GATCTCTTTG CTCCACTCGT TGTGAGATAC GTCGACCTCA TGGAGTCCTC AATTGCACAG
    TCAATTCACA GGGGCTTTGA GCGTGAATCA TGGGAACCAG TAAAGAGTTT AACAAGTAAT
    CTACCCAATG TGAATCTACC AAATGTGAAT CTCCCTAAAG TACCAGTTAC CTTACCAGTT
    AACCTCCCAC AAATGCCTAG TTTTTCTGCA CCGTCATGGA TGGCAGCTAT ATATGATTCG
    GACAATGGAT CAGCAACTTC AGAAGATCTA TTCTGGAAGC TTGACGCTCT GCAAACGTTC
    ATTCGAGATC TTCATTGGCC CGAGGAGGAA TTCGGGAAAC ACCTAGAGCA ACGCCTTAAA
    CTTATGGCTA GCGATATGAT CGAATCGTGT GTCAAACGAA CTAGAATTGC ATTCGAAGTC
    AAACTGCAGA AAACCAGTCG ATCAACAGAC TTCCGAGTAC CGCAATCAAT ATGCACCATG
    TTTAATGTTA TGGTTGATGC CAAAGCTCAG TCAACGAAAC TTTGTAGCAT GGAAATGGGC
    CAAGAGCACC AGTACCACTC CCAAATTGAC GAGCTAATTG AAGAAACAGT TAAAGAGATG
    ATCACACTCT TAGTGGCAAA GTTTGTCACA ATTCTAGAAG GAGTTCTGTC CAAACTATCC
    AGATACGATG AAGGGACATT ATTTTCCTCC TTTCTGTCAT TTACTGTGAA AGCAGCTTCC
    AAGTATGTGG ATGTACCAAA ACCCGGGATG GACCTTGCCG ACGCCTACGT GACATTTGTG
    CGCCACTCTC AGGATGTCCT GCGTGATAAG GTCAATGAGG AGATATATAT AGAAAGGTTA
    TTTGATAAAA GGCTGGATGG TAATAGTTCT GTCATGTACC TGAGAATATT GGAGCAATGG
    TATACCAGCT CAATGAATGT TGTTTGTACT TGGCTGACCG ACCGTATGGA TCTGCAACTT
    CACATTTATC AGCTGAAAAC CCTAATTAGG GTCGTCAAGA AAACCTACCG CGATTTCCGA
    TTGCAAGGCG TCTTGGACTC CACCTTGAAC AGCAAGACCT ACGACACCAT TAGAAACCGC
    CTCACCGTGG AAGAAGCCAC AGCGTCCGTG AGCGAGGGGG GAGGCCTCCA GGGAATCACC
    ATGAAGGACA GCGACGAGGA GGATGAGGAG GATGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017948371.1
    CDS746..4804
    Translation

    Target ORF information:

    RefSeq Version XM_018092882.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca++-dependent secretion activator (cadps), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092882.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTATG CTGATAGGGC ACAAAAACAT GGCATGGATG AATTTATCTC GTCCAATCCC
    TGTAACTTTG ACCACGCCAC CCTCTTTGAG ATGGTTCAGC GACTCACACT GGATCACAGA
    CTCAACGACT CGTACTCATG TCTGGGTTGG TTCAGTCCAG GTCAGGTCTT TGTACTCGAT
    GAATATTGTG CAAGATATGG AGTCCGAGGC TGCCATAGGC ATTTGTGTTA CCTTGGTGAT
    TTACTAGAAC GAGCAGAGAA TGGGTCAATG GTCGACCCAA CTTTACTTCA CTATAGTTTT
    GCCTTCTGCG CATCCCACGT ACATGGCAAC AGGCCTGATG GCATCGGAAC TGTAACCGTG
    GAAGAAAAAG AGCGCTTTGA AGAGATCAAA GAACGTCTCC GCTTGCTTTT GGAGAATCAG
    ATCACGCATT TTAGGTACTG CTTTCCATTT GGCCGACCAG AAGGTGCTTT GAAAGCCACT
    CTGTCATTGC TGGAAAGGGT TTTAATGAAA GACATAGTAA CTCCCGTCCC TCAAGAAGAA
    GTGAAAAACG TTATCCGTAA ATGCTTAGAG CAAGCAGCCT TAGTTAACTA CACGAGATTA
    TCCGAATATG CCAAAATCGA AGGGAAAAAG AGAGAAATGT ATGAACATCC TGTCTTCTGC
    TTGGCCTCCC AAGTGATGGA TTTAACCATT CAGAATCAAA AGGACGCAGA AAATGTAGGC
    CGATTAGTAA CGCCTGCCAA AAAGCTCGAA GATACAATAC GGCTCGCAGA ACTTGTCATT
    GAAGTTCTAC AGCAGAATGA GGAGCACCAT GCGGAGGGGA AAGAGGCGTT TGCTTGGTGG
    TCGGACTTGA TGGTGGAACA CGCAGAGACC TTCCTTTCGC TTTTCGCGGT GGATATGGAT
    GCTGCTTTAG AGGTGCAACC GCCAGACAGC TGGGACAGCT TCCCTCTTTT TCAGCTAATA
    AATGACTTCC TTCGAAGTGA CTATAATTTA TGCAATGGGA AATTCCACAA ACACCTTCAA
    GATCTCTTTG CTCCACTCGT TGTGAGATAC GTCGACCTCA TGGAGTCCTC AATTGCACAG
    TCAATTCACA GGGGCTTTGA GCGTGAATCA TGGGAACCAG TAAAGAGTTT AACAAGTAAT
    CTACCCAATG TGAATCTACC AAATGTGAAT CTCCCTAAAG TACCAGTTAC CTTACCAGTT
    AACCTCCCAC AAATGCCTAG TTTTTCTGCA CCGTCATGGA TGGCAGCTAT ATATGATTCG
    GACAATGGAT CAGCAACTTC AGAAGATCTA TTCTGGAAGC TTGACGCTCT GCAAACGTTC
    ATTCGAGATC TTCATTGGCC CGAGGAGGAA TTCGGGAAAC ACCTAGAGCA ACGCCTTAAA
    CTTATGGCTA GCGATATGAT CGAATCGTGT GTCAAACGAA CTAGAATTGC ATTCGAAGTC
    AAACTGCAGA AAACCAGTCG ATCAACAGAC TTCCGAGTAC CGCAATCAAT ATGCACCATG
    TTTAATGTTA TGGTTGATGC CAAAGCTCAG TCAACGAAAC TTTGTAGCAT GGAAATGGGC
    CAAGAGCACC AGTACCACTC CCAAATTGAC GAGCTAATTG AAGAAACAGT TAAAGAGATG
    ATCACACTCT TAGTGGCAAA GTTTGTCACA ATTCTAGAAG GAGTTCTGTC CAAACTATCC
    AGATACGATG AAGGGACATT ATTTTCCTCC TTTCTGTCAT TTACTGTGAA AGCAGCTTCC
    AAGTATGTGG ATGTACCAAA ACCCGGGATG GACCTTGCCG ACGCCTACGT GACATTTGTG
    CGCCACTCTC AGGATGTCCT GCGTGATAAG GTCAATGAGG AGATATATAT AGAAAGGTTA
    TTTGATAAAA GGCTGGATGG TAATAGTTCT GTCATGTACC TGAGAATATT GGAGCAATGG
    TATACCAGCT CAATGAATGT TGTTTGTACT TGGCTGACCG ACCGTATGGA TCTGCAACTT
    CACATTTATC AGCTGAAAAC CCTAATTAGG GTCGTCAAGA AAACCTACCG CGATTTCCGA
    TTGCAAGGCG TCTTGGACTC CACCTTGAAC AGCAAGACCT ACGACACCAT TAGAAACCGC
    CTCACCGTGG AAGAAGCCAC AGCGTCCGTG AGCGAGGGGG GAGGCCTCCA GGGAATCACC
    ATGAAGGACA GCGACGAGGA GGATGAGGAG GATGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa43700
    Clone ID Related Accession (Same CDS sequence) XM_018092883.1
    Accession Version XM_018092883.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4038bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGGGAAAA AGAGAGAAAT GTATGAACAT
    CCTGTCTTCT GCTTGGCCTC CCAAGTGATG GATTTAACCA TTCAGAATCA AAAGGACGCA
    GAAAATGTAG GCCGATTAGT AACGCCTGCC AAAAAGCTCG AAGATACAAT ACGGCTCGCA
    GAACTTGTCA TTGAAGTTCT ACAGCAGAAT GAGGAGCACC ATGCGGAGGG GAAAGAGGCG
    TTTGCTTGGT GGTCGGACTT GATGGTGGAA CACGCAGAGA CCTTCCTTTC GCTTTTCGCG
    GTGGATATGG ATGCTGCTTT AGAGGTGCAA CCGCCAGACA GCTGGGACAG CTTCCCTCTT
    TTTCAGCTAA TAAATGACTT CCTTCGAAGT GACTATAATT TATGCAATGG GAAATTCCAC
    AAACACCTTC AAGATCTCTT TGCTCCACTC GTTGTGAGAT ACGTCGACCT CATGGAGTCC
    TCAATTGCAC AGTCAATTCA CAGGGGCTTT GAGCGTGAAT CATGGGAACC AGTAAAGAGT
    TTAACAAGTA ATCTACCCAA TGTGAATCTA CCAAATGTGA ATCTCCCTAA AGTACCAGTT
    ACCTTACCAG TTAACCTCCC ACAAATGCCT AGTTTTTCTG CACCGTCATG GATGGCAGCT
    ATATATGATT CGGACAATGG ATCAGCAACT TCAGAAGATC TATTCTGGAA GCTTGACGCT
    CTGCAAACGT TCATTCGAGA TCTTCATTGG CCCGAGGAGG AATTCGGGAA ACACCTAGAG
    CAACGCCTTA AACTTATGGC TAGCGATATG ATCGAATCGT GTGTCAAACG AACTAGAATT
    GCATTCGAAG TCAAACTGCA GAAAACCAGT CGATCAACAG ACTTCCGAGT ACCGCAATCA
    ATATGCACCA TGTTTAATGT TATGGTTGAT GCCAAAGCTC AGTCAACGAA ACTTTGTAGC
    ATGGAAATGG GCCAAGAGCA CCAGTACCAC TCCCAAATTG ACGAGCTAAT TGAAGAAACA
    GTTAAAGAGA TGATCACACT CTTAGTGGCA AAGTTTGTCA CAATTCTAGA AGGAGTTCTG
    TCCAAACTAT CCAGATACGA TGAAGGGACA TTATTTTCCT CCTTTCTGTC ATTTACTAAA
    CCCGGGATGG ACCTTGCCGA CGCCTACGTG ACATTTGTGC GCCACTCTCA GGATGTCCTG
    CGTGATAAGG TCAATGAGGA GATATATATA GAAAGGTTAT TTGATAAAAG GCTGGATGGT
    AATAGTTCTG TCATGTACCT GAGAATATTG GAGCAATGGT ATACCAGCTC AATGAATGTT
    GTTTGTACTT GGCTGACCGA CCGTATGGAT CTGCAACTTC ACATTTATCA GCTGAAAACC
    CTAATTAGGG TCGTCAAGAA AACCTACCGC GATTTCCGAT TGCAAGGCGT CTTGGACTCC
    ACCTTGAACA GCAAGACCTA CGACACCATT AGAAACCGCC TCACCGTGGA AGAAGCCACA
    GCGTCCGTGA GCGAGGGGGG AGGCCTCCAG GGAATCACCA TGAAGGACAG CGACGAGGAG
    GATGAGGAGG ATGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017948372.1
    CDS755..4792
    Translation

    Target ORF information:

    RefSeq Version XM_018092883.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca++-dependent secretion activator (cadps), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092883.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGGGAAAA AGAGAGAAAT GTATGAACAT
    CCTGTCTTCT GCTTGGCCTC CCAAGTGATG GATTTAACCA TTCAGAATCA AAAGGACGCA
    GAAAATGTAG GCCGATTAGT AACGCCTGCC AAAAAGCTCG AAGATACAAT ACGGCTCGCA
    GAACTTGTCA TTGAAGTTCT ACAGCAGAAT GAGGAGCACC ATGCGGAGGG GAAAGAGGCG
    TTTGCTTGGT GGTCGGACTT GATGGTGGAA CACGCAGAGA CCTTCCTTTC GCTTTTCGCG
    GTGGATATGG ATGCTGCTTT AGAGGTGCAA CCGCCAGACA GCTGGGACAG CTTCCCTCTT
    TTTCAGCTAA TAAATGACTT CCTTCGAAGT GACTATAATT TATGCAATGG GAAATTCCAC
    AAACACCTTC AAGATCTCTT TGCTCCACTC GTTGTGAGAT ACGTCGACCT CATGGAGTCC
    TCAATTGCAC AGTCAATTCA CAGGGGCTTT GAGCGTGAAT CATGGGAACC AGTAAAGAGT
    TTAACAAGTA ATCTACCCAA TGTGAATCTA CCAAATGTGA ATCTCCCTAA AGTACCAGTT
    ACCTTACCAG TTAACCTCCC ACAAATGCCT AGTTTTTCTG CACCGTCATG GATGGCAGCT
    ATATATGATT CGGACAATGG ATCAGCAACT TCAGAAGATC TATTCTGGAA GCTTGACGCT
    CTGCAAACGT TCATTCGAGA TCTTCATTGG CCCGAGGAGG AATTCGGGAA ACACCTAGAG
    CAACGCCTTA AACTTATGGC TAGCGATATG ATCGAATCGT GTGTCAAACG AACTAGAATT
    GCATTCGAAG TCAAACTGCA GAAAACCAGT CGATCAACAG ACTTCCGAGT ACCGCAATCA
    ATATGCACCA TGTTTAATGT TATGGTTGAT GCCAAAGCTC AGTCAACGAA ACTTTGTAGC
    ATGGAAATGG GCCAAGAGCA CCAGTACCAC TCCCAAATTG ACGAGCTAAT TGAAGAAACA
    GTTAAAGAGA TGATCACACT CTTAGTGGCA AAGTTTGTCA CAATTCTAGA AGGAGTTCTG
    TCCAAACTAT CCAGATACGA TGAAGGGACA TTATTTTCCT CCTTTCTGTC ATTTACTAAA
    CCCGGGATGG ACCTTGCCGA CGCCTACGTG ACATTTGTGC GCCACTCTCA GGATGTCCTG
    CGTGATAAGG TCAATGAGGA GATATATATA GAAAGGTTAT TTGATAAAAG GCTGGATGGT
    AATAGTTCTG TCATGTACCT GAGAATATTG GAGCAATGGT ATACCAGCTC AATGAATGTT
    GTTTGTACTT GGCTGACCGA CCGTATGGAT CTGCAACTTC ACATTTATCA GCTGAAAACC
    CTAATTAGGG TCGTCAAGAA AACCTACCGC GATTTCCGAT TGCAAGGCGT CTTGGACTCC
    ACCTTGAACA GCAAGACCTA CGACACCATT AGAAACCGCC TCACCGTGGA AGAAGCCACA
    GCGTCCGTGA GCGAGGGGGG AGGCCTCCAG GGAATCACCA TGAAGGACAG CGACGAGGAG
    GATGAGGAGG ATGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa43701
    Clone ID Related Accession (Same CDS sequence) XM_018092884.1
    Accession Version XM_018092884.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4035bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGGGAAAA AGAGAGAAAT GTATGAACAT
    CCTGTCTTCT GCTTGGCCTC CCAAGTGATG GATTTAACCA TTCAGAATCA AAAGGACGCA
    GAAAATGTAG GCCGATTAGT AACGCCTGCC AAAAAGCTCG AAGATACAAT ACGGCTCGCA
    GAACTTGTCA TTGAAGTTCT ACAGCAGAAT GAGGAGCACC ATGCGGAGGG GAAAGAGGCG
    TTTGCTTGGT GGTCGGACTT GATGGTGGAA CACGCAGAGA CCTTCCTTTC GCTTTTCGCG
    GTGGATATGG ATGCTGCTTT AGAGGTGCAA CCGCCAGACA GCTGGGACAG CTTCCCTCTT
    TTTCAGCTAA TAAATGACTT CCTTCGAAGT GACTATAATT TATGCAATGG GAAATTCCAC
    AAACACCTTC AAGATCTCTT TGCTCCACTC GTTGTGAGAT ACGTCGACCT CATGGAGTCC
    TCAATTGCAC AGTCAATTCA CAGGGGCTTT GAGCGTGAAT CATGGGAACC AGTAAAGAGT
    TTAACAAGTA ATCTACCCAA TGTGAATCTA CCAAATGTGA ATCTCCCTAA AGTACCAGTT
    ACCTTACCAG TTAACCTCCC ACAAATGCCT AGTTTTTCTG CACCGTCATG GATGGCAGCT
    ATATATGATT CGGACAATGG ATCAGCAACT TCAGAAGATC TATTCTGGAA GCTTGACGCT
    CTGCAAACGT TCATTCGAGA TCTTCATTGG CCCGAGGAGG AATTCGGGAA ACACCTAGAG
    CAACGCCTTA AACTTATGGC TAGCGATATG ATCGAATCGT GTGTCAAACG AACTAGAATT
    GCATTCGAAG TCAAACTGCA GAAAACCAGT CGATCAACAG ACTTCCGAGT ACCGCAATCA
    ATATGCACCA TGTTTAATGT TATGGTTGAT GCCAAAGCTC AGTCAACGAA ACTTTGTAGC
    ATGGAAATGG GCCAAGAGCA CCAGTACCAC TCCCAAATTG ACGAGCTAAT TGAAGAAACA
    GTTAAAGAGA TGATCACACT CTTAGTGGCA AAGTTTGTCA CAATTCTAGA AGGAGTTCTG
    TCCAAACTAT CCAGATACGA TGAAGGGACA TTATTTTCCT CCTTTCTGTC ATTTACTGTG
    AAAGCAGCTT CCAAGTATGT GGATGTACCA AAACCCGGGA TGGACCTTGC CGACGCCTAC
    GTGACATTTG TGCGCCACTC TCAGGATGTC CTGCGTGATA AGAAAAGGCT GGATGGTAAT
    AGTTCTGTCA TGTACCTGAG AATATTGGAG CAATGGTATA CCAGCTCAAT GAATGTTGTT
    TGTACTTGGC TGACCGACCG TATGGATCTG CAACTTCACA TTTATCAGCT GAAAACCCTA
    ATTAGGGTCG TCAAGAAAAC CTACCGCGAT TTCCGATTGC AAGGCGTCTT GGACTCCACC
    TTGAACAGCA AGACCTACGA CACCATTAGA AACCGCCTCA CCGTGGAAGA AGCCACAGCG
    TCCGTGAGCG AGGGGGGAGG CCTCCAGGGA ATCACCATGA AGGACAGCGA CGAGGAGGAT
    GAGGAGGATG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017948373.1
    CDS752..4786
    Translation

    Target ORF information:

    RefSeq Version XM_018092884.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca++-dependent secretion activator (cadps), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092884.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGGGAAAA AGAGAGAAAT GTATGAACAT
    CCTGTCTTCT GCTTGGCCTC CCAAGTGATG GATTTAACCA TTCAGAATCA AAAGGACGCA
    GAAAATGTAG GCCGATTAGT AACGCCTGCC AAAAAGCTCG AAGATACAAT ACGGCTCGCA
    GAACTTGTCA TTGAAGTTCT ACAGCAGAAT GAGGAGCACC ATGCGGAGGG GAAAGAGGCG
    TTTGCTTGGT GGTCGGACTT GATGGTGGAA CACGCAGAGA CCTTCCTTTC GCTTTTCGCG
    GTGGATATGG ATGCTGCTTT AGAGGTGCAA CCGCCAGACA GCTGGGACAG CTTCCCTCTT
    TTTCAGCTAA TAAATGACTT CCTTCGAAGT GACTATAATT TATGCAATGG GAAATTCCAC
    AAACACCTTC AAGATCTCTT TGCTCCACTC GTTGTGAGAT ACGTCGACCT CATGGAGTCC
    TCAATTGCAC AGTCAATTCA CAGGGGCTTT GAGCGTGAAT CATGGGAACC AGTAAAGAGT
    TTAACAAGTA ATCTACCCAA TGTGAATCTA CCAAATGTGA ATCTCCCTAA AGTACCAGTT
    ACCTTACCAG TTAACCTCCC ACAAATGCCT AGTTTTTCTG CACCGTCATG GATGGCAGCT
    ATATATGATT CGGACAATGG ATCAGCAACT TCAGAAGATC TATTCTGGAA GCTTGACGCT
    CTGCAAACGT TCATTCGAGA TCTTCATTGG CCCGAGGAGG AATTCGGGAA ACACCTAGAG
    CAACGCCTTA AACTTATGGC TAGCGATATG ATCGAATCGT GTGTCAAACG AACTAGAATT
    GCATTCGAAG TCAAACTGCA GAAAACCAGT CGATCAACAG ACTTCCGAGT ACCGCAATCA
    ATATGCACCA TGTTTAATGT TATGGTTGAT GCCAAAGCTC AGTCAACGAA ACTTTGTAGC
    ATGGAAATGG GCCAAGAGCA CCAGTACCAC TCCCAAATTG ACGAGCTAAT TGAAGAAACA
    GTTAAAGAGA TGATCACACT CTTAGTGGCA AAGTTTGTCA CAATTCTAGA AGGAGTTCTG
    TCCAAACTAT CCAGATACGA TGAAGGGACA TTATTTTCCT CCTTTCTGTC ATTTACTGTG
    AAAGCAGCTT CCAAGTATGT GGATGTACCA AAACCCGGGA TGGACCTTGC CGACGCCTAC
    GTGACATTTG TGCGCCACTC TCAGGATGTC CTGCGTGATA AGAAAAGGCT GGATGGTAAT
    AGTTCTGTCA TGTACCTGAG AATATTGGAG CAATGGTATA CCAGCTCAAT GAATGTTGTT
    TGTACTTGGC TGACCGACCG TATGGATCTG CAACTTCACA TTTATCAGCT GAAAACCCTA
    ATTAGGGTCG TCAAGAAAAC CTACCGCGAT TTCCGATTGC AAGGCGTCTT GGACTCCACC
    TTGAACAGCA AGACCTACGA CACCATTAGA AACCGCCTCA CCGTGGAAGA AGCCACAGCG
    TCCGTGAGCG AGGGGGGAGG CCTCCAGGGA ATCACCATGA AGGACAGCGA CGAGGAGGAT
    GAGGAGGATG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa43702
    Clone ID Related Accession (Same CDS sequence) XM_018092885.1
    Accession Version XM_018092885.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4023bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGGGAAAA AGAGAGAAAT GTATGAACAT
    CCTGTCTTCT GCTTGGCCTC CCAAGTGATG GATTTAACCA TTCAGAATCA AAAGGACGCA
    GAAAATGTAG GCCGATTAGT AACGCCTGCC AAAAAGCTCG AAGATACAAT ACGGCTCGCA
    GAACTTGTCA TTGAAGTTCT ACAGCAGAAT GAGGAGCACC ATGCGGAGGG GAAAGAGGCG
    TTTGCTTGGT GGTCGGACTT GATGGTGGAA CACGCAGAGA CCTTCCTTTC GCTTTTCGCG
    GTGGATATGG ATGCTGCTTT AGAGGTGCAA CCGCCAGACA GCTGGGACAG CTTCCCTCTT
    TTTCAGCTAA TAAATGACTT CCTTCGAAGT GACTATAATT TATGCAATGG GAAATTCCAC
    AAACACCTTC AAGATCTCTT TGCTCCACTC GTTGTGAGAT ACGTCGACCT CATGGAGTCC
    TCAATTGCAC AGTCAATTCA CAGGGGCTTT GAGCGTGAAT CATGGGAACC AGTAAAGAGT
    TTAACAAGTA ATCTACCCAA TGTGAATCTA CCAAATGTGA ATCTCCCTAA AGTACCAGTT
    ACCTTACCAG TTAACCTCCC ACAAATGCCT AGTTTTTCTG CACCGTCATG GATGGCAGCT
    ATATATGATT CGGACAATGG ATCAGCAACT TCAGAAGATC TATTCTGGAA GCTTGACGCT
    CTGCAAACGT TCATTCGAGA TCTTCATTGG CCCGAGGAGG AATTCGGGAA ACACCTAGAG
    CAACGCCTTA AACTTATGGC TAGCGATATG ATCGAATCGT GTGTCAAACG AACTAGAATT
    GCATTCGAAG TCAAACTGCA GAAAACCAGT CGATCAACAG ACTTCCGAGT ACCGCAATCA
    ATATGCACCA TGTTTAATGT TATGGTTGAT GCCAAAGCTC AGTCAACGAA ACTTTGTAGC
    ATGGAAATGG GCCAAGAGCA CCAGTACCAC TCCCAAATTG ACGAGCTAAT TGAAGAAACA
    GTTAAAGAGA TGATCACACT CTTAGTGGCA AAGTTTGTCA CAATTCTAGA AGGAGTTCTG
    TCCAAACTAT CCAGATACGA TGAAGGGACA TTATTTTCCT CCTTTCTGTC ATTTACTGTG
    AAAGCAGCTT CCAAGTATGT GGATGTACCA AAACCCGGGA TGGACCTTGC CGACGCCTAC
    GTGACATTTG TGCGCCACTC TCAGGATGTC CTGCGTGATA AGGTCAATGA GGAGATATAT
    ATAGAAAGGT TATTTGATCA ATGGTATACC AGCTCAATGA ATGTTGTTTG TACTTGGCTG
    ACCGACCGTA TGGATCTGCA ACTTCACATT TATCAGCTGA AAACCCTAAT TAGGGTCGTC
    AAGAAAACCT ACCGCGATTT CCGATTGCAA GGCGTCTTGG ACTCCACCTT GAACAGCAAG
    ACCTACGACA CCATTAGAAA CCGCCTCACC GTGGAAGAAG CCACAGCGTC CGTGAGCGAG
    GGGGGAGGCC TCCAGGGAAT CACCATGAAG GACAGCGACG AGGAGGATGA GGAGGATGAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017948374.1
    CDS748..4770
    Translation

    Target ORF information:

    RefSeq Version XM_018092885.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca++-dependent secretion activator (cadps), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092885.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGGGAAAA AGAGAGAAAT GTATGAACAT
    CCTGTCTTCT GCTTGGCCTC CCAAGTGATG GATTTAACCA TTCAGAATCA AAAGGACGCA
    GAAAATGTAG GCCGATTAGT AACGCCTGCC AAAAAGCTCG AAGATACAAT ACGGCTCGCA
    GAACTTGTCA TTGAAGTTCT ACAGCAGAAT GAGGAGCACC ATGCGGAGGG GAAAGAGGCG
    TTTGCTTGGT GGTCGGACTT GATGGTGGAA CACGCAGAGA CCTTCCTTTC GCTTTTCGCG
    GTGGATATGG ATGCTGCTTT AGAGGTGCAA CCGCCAGACA GCTGGGACAG CTTCCCTCTT
    TTTCAGCTAA TAAATGACTT CCTTCGAAGT GACTATAATT TATGCAATGG GAAATTCCAC
    AAACACCTTC AAGATCTCTT TGCTCCACTC GTTGTGAGAT ACGTCGACCT CATGGAGTCC
    TCAATTGCAC AGTCAATTCA CAGGGGCTTT GAGCGTGAAT CATGGGAACC AGTAAAGAGT
    TTAACAAGTA ATCTACCCAA TGTGAATCTA CCAAATGTGA ATCTCCCTAA AGTACCAGTT
    ACCTTACCAG TTAACCTCCC ACAAATGCCT AGTTTTTCTG CACCGTCATG GATGGCAGCT
    ATATATGATT CGGACAATGG ATCAGCAACT TCAGAAGATC TATTCTGGAA GCTTGACGCT
    CTGCAAACGT TCATTCGAGA TCTTCATTGG CCCGAGGAGG AATTCGGGAA ACACCTAGAG
    CAACGCCTTA AACTTATGGC TAGCGATATG ATCGAATCGT GTGTCAAACG AACTAGAATT
    GCATTCGAAG TCAAACTGCA GAAAACCAGT CGATCAACAG ACTTCCGAGT ACCGCAATCA
    ATATGCACCA TGTTTAATGT TATGGTTGAT GCCAAAGCTC AGTCAACGAA ACTTTGTAGC
    ATGGAAATGG GCCAAGAGCA CCAGTACCAC TCCCAAATTG ACGAGCTAAT TGAAGAAACA
    GTTAAAGAGA TGATCACACT CTTAGTGGCA AAGTTTGTCA CAATTCTAGA AGGAGTTCTG
    TCCAAACTAT CCAGATACGA TGAAGGGACA TTATTTTCCT CCTTTCTGTC ATTTACTGTG
    AAAGCAGCTT CCAAGTATGT GGATGTACCA AAACCCGGGA TGGACCTTGC CGACGCCTAC
    GTGACATTTG TGCGCCACTC TCAGGATGTC CTGCGTGATA AGGTCAATGA GGAGATATAT
    ATAGAAAGGT TATTTGATCA ATGGTATACC AGCTCAATGA ATGTTGTTTG TACTTGGCTG
    ACCGACCGTA TGGATCTGCA ACTTCACATT TATCAGCTGA AAACCCTAAT TAGGGTCGTC
    AAGAAAACCT ACCGCGATTT CCGATTGCAA GGCGTCTTGG ACTCCACCTT GAACAGCAAG
    ACCTACGACA CCATTAGAAA CCGCCTCACC GTGGAAGAAG CCACAGCGTC CGTGAGCGAG
    GGGGGAGGCC TCCAGGGAAT CACCATGAAG GACAGCGACG AGGAGGATGA GGAGGATGAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa43703
    Clone ID Related Accession (Same CDS sequence) XM_018092886.1
    Accession Version XM_018092886.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4002bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGGGAAAA AGAGAGAAAT GTATGAACAT
    CCTGTCTTCT GCTTGGCCTC CCAAGTGATG GATTTAACCA TTCAGAATCA AAAGGACGCA
    GAAAATGTAG GCCGATTAGT AACGCCTGCC AAAAAGCTCG AAGATACAAT ACGGCTCGCA
    GAACTTGTCA TTGAAGTTCT ACAGCAGAAT GAGGAGCACC ATGCGGAGGG GAAAGAGGCG
    TTTGCTTGGT GGTCGGACTT GATGGTGGAA CACGCAGAGA CCTTCCTTTC GCTTTTCGCG
    GTGGATATGG ATGCTGCTTT AGAGGTGCAA CCGCCAGACA GCTGGGACAG CTTCCCTCTT
    TTTCAGCTAA TAAATGACTT CCTTCGAAGT GACTATAATT TATGCAATGG GAAATTCCAC
    AAACACCTTC AAGATCTCTT TGCTCCACTC GTTGTGAGAT ACGTCGACCT CATGGAGTCC
    TCAATTGCAC AGTCAATTCA CAGGGGCTTT GAGCGTGAAT CATGGGAACC AGTAAAGAGT
    TTAACAAGTA ATCTACCCAA TGTGAATCTA CCAAATGTGA ATCTCCCTAA AGTACCAGTT
    ACCTTACCAG TTAACCTCCC ACAAATGCCT AGTTTTTCTG CACCGTCATG GATGGCAGCT
    ATATATGATT CGGACAATGG ATCAGCAACT TCAGAAGATC TATTCTGGAA GCTTGACGCT
    CTGCAAACGT TCATTCGAGA TCTTCATTGG CCCGAGGAGG AATTCGGGAA ACACCTAGAG
    CAACGCCTTA AACTTATGGC TAGCGATATG ATCGAATCGT GTGTCAAACG AACTAGAATT
    GCATTCGAAG TCAAACTGCA GAAAACCAGT CGATCAACAG ACTTCCGAGT ACCGCAATCA
    ATATGCACCA TGTTTAATGT TATGGTTGAT GCCAAAGCTC AGTCAACGAA ACTTTGTAGC
    ATGGAAATGG GCCAAGAGCA CCAGTACCAC TCCCAAATTG ACGAGCTAAT TGAAGAAACA
    GTTAAAGAGA TGATCACACT CTTAGTGGCA AAGTTTGTCA CAATTCTAGA AGGAGTTCTG
    TCCAAACTAT CCAGATACGA TGAAGGGACA TTATTTTCCT CCTTTCTGTC ATTTACTAAA
    CCCGGGATGG ACCTTGCCGA CGCCTACGTG ACATTTGTGC GCCACTCTCA GGATGTCCTG
    CGTGATAAGA AAAGGCTGGA TGGTAATAGT TCTGTCATGT ACCTGAGAAT ATTGGAGCAA
    TGGTATACCA GCTCAATGAA TGTTGTTTGT ACTTGGCTGA CCGACCGTAT GGATCTGCAA
    CTTCACATTT ATCAGCTGAA AACCCTAATT AGGGTCGTCA AGAAAACCTA CCGCGATTTC
    CGATTGCAAG GCGTCTTGGA CTCCACCTTG AACAGCAAGA CCTACGACAC CATTAGAAAC
    CGCCTCACCG TGGAAGAAGC CACAGCGTCC GTGAGCGAGG GGGGAGGCCT CCAGGGAATC
    ACCATGAAGG ACAGCGACGA GGAGGATGAG GAGGATGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017948375.1
    CDS757..4758
    Translation

    Target ORF information:

    RefSeq Version XM_018092886.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca++-dependent secretion activator (cadps), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092886.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGGGAAAA AGAGAGAAAT GTATGAACAT
    CCTGTCTTCT GCTTGGCCTC CCAAGTGATG GATTTAACCA TTCAGAATCA AAAGGACGCA
    GAAAATGTAG GCCGATTAGT AACGCCTGCC AAAAAGCTCG AAGATACAAT ACGGCTCGCA
    GAACTTGTCA TTGAAGTTCT ACAGCAGAAT GAGGAGCACC ATGCGGAGGG GAAAGAGGCG
    TTTGCTTGGT GGTCGGACTT GATGGTGGAA CACGCAGAGA CCTTCCTTTC GCTTTTCGCG
    GTGGATATGG ATGCTGCTTT AGAGGTGCAA CCGCCAGACA GCTGGGACAG CTTCCCTCTT
    TTTCAGCTAA TAAATGACTT CCTTCGAAGT GACTATAATT TATGCAATGG GAAATTCCAC
    AAACACCTTC AAGATCTCTT TGCTCCACTC GTTGTGAGAT ACGTCGACCT CATGGAGTCC
    TCAATTGCAC AGTCAATTCA CAGGGGCTTT GAGCGTGAAT CATGGGAACC AGTAAAGAGT
    TTAACAAGTA ATCTACCCAA TGTGAATCTA CCAAATGTGA ATCTCCCTAA AGTACCAGTT
    ACCTTACCAG TTAACCTCCC ACAAATGCCT AGTTTTTCTG CACCGTCATG GATGGCAGCT
    ATATATGATT CGGACAATGG ATCAGCAACT TCAGAAGATC TATTCTGGAA GCTTGACGCT
    CTGCAAACGT TCATTCGAGA TCTTCATTGG CCCGAGGAGG AATTCGGGAA ACACCTAGAG
    CAACGCCTTA AACTTATGGC TAGCGATATG ATCGAATCGT GTGTCAAACG AACTAGAATT
    GCATTCGAAG TCAAACTGCA GAAAACCAGT CGATCAACAG ACTTCCGAGT ACCGCAATCA
    ATATGCACCA TGTTTAATGT TATGGTTGAT GCCAAAGCTC AGTCAACGAA ACTTTGTAGC
    ATGGAAATGG GCCAAGAGCA CCAGTACCAC TCCCAAATTG ACGAGCTAAT TGAAGAAACA
    GTTAAAGAGA TGATCACACT CTTAGTGGCA AAGTTTGTCA CAATTCTAGA AGGAGTTCTG
    TCCAAACTAT CCAGATACGA TGAAGGGACA TTATTTTCCT CCTTTCTGTC ATTTACTAAA
    CCCGGGATGG ACCTTGCCGA CGCCTACGTG ACATTTGTGC GCCACTCTCA GGATGTCCTG
    CGTGATAAGA AAAGGCTGGA TGGTAATAGT TCTGTCATGT ACCTGAGAAT ATTGGAGCAA
    TGGTATACCA GCTCAATGAA TGTTGTTTGT ACTTGGCTGA CCGACCGTAT GGATCTGCAA
    CTTCACATTT ATCAGCTGAA AACCCTAATT AGGGTCGTCA AGAAAACCTA CCGCGATTTC
    CGATTGCAAG GCGTCTTGGA CTCCACCTTG AACAGCAAGA CCTACGACAC CATTAGAAAC
    CGCCTCACCG TGGAAGAAGC CACAGCGTCC GTGAGCGAGG GGGGAGGCCT CCAGGGAATC
    ACCATGAAGG ACAGCGACGA GGAGGATGAG GAGGATGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa43704
    Clone ID Related Accession (Same CDS sequence) XM_018092887.1
    Accession Version XM_018092887.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3933bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGGGAAAA AGAGAGAAAT GTATGAACAT
    CCTGTCTTCT GCTTGGCCTC CCAAGTGATG GATTTAACCA TTCAGAATCA AAAGGACGCA
    GAAAATGTAG GCCGATTAGT AACGCCTGCC AAAAAGCTCG AAGATACAAT ACGGCTCGCA
    GAACTTGTCA TTGAAGTTCT ACAGCAGAAT GAGGAGCACC ATGCGGAGGG GAAAGAGGCG
    TTTGCTTGGT GGTCGGACTT GATGGTGGAA CACGCAGAGA CCTTCCTTTC GCTTTTCGCG
    GTGGATATGG ATGCTGCTTT AGAGGTGCAA CCGCCAGACA GCTGGGACAG CTTCCCTCTT
    TTTCAGCTAA TAAATGACTT CCTTCGAAGT GACTATAATT TATGCAATGG GAAATTCCAC
    AAACACCTTC AAGATCTCTT TGCTCCACTC GTTGTGAGAT ACGTCGACCT CATGGAGTCC
    TCAATTGCAC AGTCAATTCA CAGGGGCTTT GAGCGTGAAT CATGGGAACC AGTAAACAAT
    GGATCAGCAA CTTCAGAAGA TCTATTCTGG AAGCTTGACG CTCTGCAAAC GTTCATTCGA
    GATCTTCATT GGCCCGAGGA GGAATTCGGG AAACACCTAG AGCAACGCCT TAAACTTATG
    GCTAGCGATA TGATCGAATC GTGTGTCAAA CGAACTAGAA TTGCATTCGA AGTCAAACTG
    CAGAAAACCA GTCGATCAAC AGACTTCCGA GTACCGCAAT CAATATGCAC CATGTTTAAT
    GTTATGGTTG ATGCCAAAGC TCAGTCAACG AAACTTTGTA GCATGGAAAT GGGCCAAGAG
    CACCAGTACC ACTCCCAAAT TGACGAGCTA ATTGAAGAAA CAGTTAAAGA GATGATCACA
    CTCTTAGTGG CAAAGTTTGT CACAATTCTA GAAGGAGTTC TGTCCAAACT ATCCAGATAC
    GATGAAGGGA CATTATTTTC CTCCTTTCTG TCATTTACTG TGAAAGCAGC TTCCAAGTAT
    GTGGATGTAC CAAAACCCGG GATGGACCTT GCCGACGCCT ACGTGACATT TGTGCGCCAC
    TCTCAGGATG TCCTGCGTGA TAAGGTCAAT GAGGAGATAT ATATAGAAAG GTTATTTGAT
    AAAAGGCTGG ATGGTAATAG TTCTGTCATG TACCTGAGAA TATTGGAGCA ATGGTATACC
    AGCTCAATGA ATGTTGTTTG TACTTGGCTG ACCGACCGTA TGGATCTGCA ACTTCACATT
    TATCAGCTGA AAACCCTAAT TAGGGTCGTC AAGAAAACCT ACCGCGATTT CCGATTGCAA
    GGCGTCTTGG ACTCCACCTT GAACAGCAAG ACCTACGACA CCATTAGAAA CCGCCTCACC
    GTGGAAGAAG CCACAGCGTC CGTGAGCGAG GGGGGAGGCC TCCAGGGAAT CACCATGAAG
    GACAGCGACG AGGAGGATGA GGAGGATGAT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017948376.1
    CDS746..4678
    Translation

    Target ORF information:

    RefSeq Version XM_018092887.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca++-dependent secretion activator (cadps), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092887.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGGGAAAA AGAGAGAAAT GTATGAACAT
    CCTGTCTTCT GCTTGGCCTC CCAAGTGATG GATTTAACCA TTCAGAATCA AAAGGACGCA
    GAAAATGTAG GCCGATTAGT AACGCCTGCC AAAAAGCTCG AAGATACAAT ACGGCTCGCA
    GAACTTGTCA TTGAAGTTCT ACAGCAGAAT GAGGAGCACC ATGCGGAGGG GAAAGAGGCG
    TTTGCTTGGT GGTCGGACTT GATGGTGGAA CACGCAGAGA CCTTCCTTTC GCTTTTCGCG
    GTGGATATGG ATGCTGCTTT AGAGGTGCAA CCGCCAGACA GCTGGGACAG CTTCCCTCTT
    TTTCAGCTAA TAAATGACTT CCTTCGAAGT GACTATAATT TATGCAATGG GAAATTCCAC
    AAACACCTTC AAGATCTCTT TGCTCCACTC GTTGTGAGAT ACGTCGACCT CATGGAGTCC
    TCAATTGCAC AGTCAATTCA CAGGGGCTTT GAGCGTGAAT CATGGGAACC AGTAAACAAT
    GGATCAGCAA CTTCAGAAGA TCTATTCTGG AAGCTTGACG CTCTGCAAAC GTTCATTCGA
    GATCTTCATT GGCCCGAGGA GGAATTCGGG AAACACCTAG AGCAACGCCT TAAACTTATG
    GCTAGCGATA TGATCGAATC GTGTGTCAAA CGAACTAGAA TTGCATTCGA AGTCAAACTG
    CAGAAAACCA GTCGATCAAC AGACTTCCGA GTACCGCAAT CAATATGCAC CATGTTTAAT
    GTTATGGTTG ATGCCAAAGC TCAGTCAACG AAACTTTGTA GCATGGAAAT GGGCCAAGAG
    CACCAGTACC ACTCCCAAAT TGACGAGCTA ATTGAAGAAA CAGTTAAAGA GATGATCACA
    CTCTTAGTGG CAAAGTTTGT CACAATTCTA GAAGGAGTTC TGTCCAAACT ATCCAGATAC
    GATGAAGGGA CATTATTTTC CTCCTTTCTG TCATTTACTG TGAAAGCAGC TTCCAAGTAT
    GTGGATGTAC CAAAACCCGG GATGGACCTT GCCGACGCCT ACGTGACATT TGTGCGCCAC
    TCTCAGGATG TCCTGCGTGA TAAGGTCAAT GAGGAGATAT ATATAGAAAG GTTATTTGAT
    AAAAGGCTGG ATGGTAATAG TTCTGTCATG TACCTGAGAA TATTGGAGCA ATGGTATACC
    AGCTCAATGA ATGTTGTTTG TACTTGGCTG ACCGACCGTA TGGATCTGCA ACTTCACATT
    TATCAGCTGA AAACCCTAAT TAGGGTCGTC AAGAAAACCT ACCGCGATTT CCGATTGCAA
    GGCGTCTTGG ACTCCACCTT GAACAGCAAG ACCTACGACA CCATTAGAAA CCGCCTCACC
    GTGGAAGAAG CCACAGCGTC CGTGAGCGAG GGGGGAGGCC TCCAGGGAAT CACCATGAAG
    GACAGCGACG AGGAGGATGA GGAGGATGAT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55636
    Clone ID Related Accession (Same CDS sequence) XM_012960425.3
    Accession Version XM_012960425.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4071bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012960425.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGGGAAAA AGAGAGAAAT GTATGAACAT
    CCTGTCTTCT GCTTGGCCTC CCAAGTGATG GATTTAACCA TTCAGAATCA AAAGGACGCA
    GAAAATGTAG GCCGATTAGT AACGCCTGCC AAAAAGCTCG AAGATACAAT ACGGCTCGCA
    GAACTTGTCA TTGAAGTTCT ACAGCAGAAT GAGGAGCACC ATGCGGAGGG GAAAGAGGCG
    TTTGCTTGGT GGTCGGACTT GATGGTGGAA CACGCAGAGA CCTTCCTTTC GCTTTTCGCG
    GTGGATATGG ATGCTGCTTT AGAGGTGCAA CCGCCAGACA GCTGGGACAG CTTCCCTCTT
    TTTCAGCTAA TAAATGACTT CCTTCGAAGT GACTATAATT TATGCAATGG GAAATTCCAC
    AAACACCTTC AAGATCTCTT TGCTCCACTC GTTGTGAGAT ACGTCGACCT CATGGAGTCC
    TCAATTGCAC AGTCAATTCA CAGGGGCTTT GAGCGTGAAT CATGGGAACC AGTAAAGAGT
    TTAACAAGTA ATCTACCCAA TGTGAATCTA CCAAATGTGA ATCTCCCTAA AGTACCAGTT
    ACCTTACCAG TTAACCTCCC ACAAATGCCT AGTTTTTCTG CACCGTCATG GATGGCAGCT
    ATATATGATT CGGACAATGG ATCAGCAACT TCGGAAGATC TATTCTGGAA GCTTGACGCT
    CTGCAAACGT TCATTCGAGA TCTTCATTGG CCCGAGGAGG AATTCGGGAA ACACCTAGAG
    CAACGCCTTA AACTTATGGC TAGCGATATG ATCGAATCGT GTGTCAAACG AACTAGAATT
    GCATTCGAAG TCAAACTGCA GAAAACCAGT CGATCAACAG ACTTCCGAGT ACCGCAATCA
    ATATGCACCA TGTTTAATGT TATGGTTGAT GCCAAAGCTC AGTCAACGAA ACTTTGTAGC
    ATGGAAATGG GCCAAGAGCA CCAGTACCAC TCCCAAATTG ACGAGCTAAT TGAAGAAACA
    GTTAAAGAGA TGATCACACT CTTAGTGGCA AAGTTTGTCA CAATTCTAGA AGGAGTTCTG
    TCCAAACTAT CCAGATACGA TGAAGGGACA TTATTTTCCT CCTTTCTGTC ATTTACTGTG
    AAAGCAGCTT CCAAGTATGT GGATGTACCA AAACCCGGGA TGGACCTTGC CGACGCCTAC
    GTGACATTTG TGCGCCACTC TCAGGATGTC CTGCGTGATA AGGTCAATGA GGAGATATAT
    ATAGAAAGGT TATTTGATAA AAGGCTGGAT GGTAATAGTT CTGTCATGTA CCTGAGAATA
    TTGGAGCAAT GGTATACCAG CTCAATGAAT GTTGTTTGTA CTTGGCTGAC CGACCGTATG
    GATCTGCAAC TTCACATTTA TCAGCTGAAA ACCCTAATTA GGGTCGTCAA GAAAACCTAC
    CGCGATTTCC GATTGCAAGG CGTCTTGGAC TCCACCTTGA ACAGCAAGAC CTACGACACC
    ATTAGAAACC GCCTCACCGT GGAAGAAGCC ACAGCGTCCG TGAGCGAGGG GGGAGGCCTC
    CAGGGAATCA CCATGAAGGA CAGCGACGAG GAGGATGAGG AGGATGATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815879.1
    CDS601..4671
    Translation

    Target ORF information:

    RefSeq Version XM_012960425.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012960425.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGGGAAAA AGAGAGAAAT GTATGAACAT
    CCTGTCTTCT GCTTGGCCTC CCAAGTGATG GATTTAACCA TTCAGAATCA AAAGGACGCA
    GAAAATGTAG GCCGATTAGT AACGCCTGCC AAAAAGCTCG AAGATACAAT ACGGCTCGCA
    GAACTTGTCA TTGAAGTTCT ACAGCAGAAT GAGGAGCACC ATGCGGAGGG GAAAGAGGCG
    TTTGCTTGGT GGTCGGACTT GATGGTGGAA CACGCAGAGA CCTTCCTTTC GCTTTTCGCG
    GTGGATATGG ATGCTGCTTT AGAGGTGCAA CCGCCAGACA GCTGGGACAG CTTCCCTCTT
    TTTCAGCTAA TAAATGACTT CCTTCGAAGT GACTATAATT TATGCAATGG GAAATTCCAC
    AAACACCTTC AAGATCTCTT TGCTCCACTC GTTGTGAGAT ACGTCGACCT CATGGAGTCC
    TCAATTGCAC AGTCAATTCA CAGGGGCTTT GAGCGTGAAT CATGGGAACC AGTAAAGAGT
    TTAACAAGTA ATCTACCCAA TGTGAATCTA CCAAATGTGA ATCTCCCTAA AGTACCAGTT
    ACCTTACCAG TTAACCTCCC ACAAATGCCT AGTTTTTCTG CACCGTCATG GATGGCAGCT
    ATATATGATT CGGACAATGG ATCAGCAACT TCGGAAGATC TATTCTGGAA GCTTGACGCT
    CTGCAAACGT TCATTCGAGA TCTTCATTGG CCCGAGGAGG AATTCGGGAA ACACCTAGAG
    CAACGCCTTA AACTTATGGC TAGCGATATG ATCGAATCGT GTGTCAAACG AACTAGAATT
    GCATTCGAAG TCAAACTGCA GAAAACCAGT CGATCAACAG ACTTCCGAGT ACCGCAATCA
    ATATGCACCA TGTTTAATGT TATGGTTGAT GCCAAAGCTC AGTCAACGAA ACTTTGTAGC
    ATGGAAATGG GCCAAGAGCA CCAGTACCAC TCCCAAATTG ACGAGCTAAT TGAAGAAACA
    GTTAAAGAGA TGATCACACT CTTAGTGGCA AAGTTTGTCA CAATTCTAGA AGGAGTTCTG
    TCCAAACTAT CCAGATACGA TGAAGGGACA TTATTTTCCT CCTTTCTGTC ATTTACTGTG
    AAAGCAGCTT CCAAGTATGT GGATGTACCA AAACCCGGGA TGGACCTTGC CGACGCCTAC
    GTGACATTTG TGCGCCACTC TCAGGATGTC CTGCGTGATA AGGTCAATGA GGAGATATAT
    ATAGAAAGGT TATTTGATAA AAGGCTGGAT GGTAATAGTT CTGTCATGTA CCTGAGAATA
    TTGGAGCAAT GGTATACCAG CTCAATGAAT GTTGTTTGTA CTTGGCTGAC CGACCGTATG
    GATCTGCAAC TTCACATTTA TCAGCTGAAA ACCCTAATTA GGGTCGTCAA GAAAACCTAC
    CGCGATTTCC GATTGCAAGG CGTCTTGGAC TCCACCTTGA ACAGCAAGAC CTACGACACC
    ATTAGAAACC GCCTCACCGT GGAAGAAGCC ACAGCGTCCG TGAGCGAGGG GGGAGGCCTC
    CAGGGAATCA CCATGAAGGA CAGCGACGAG GAGGATGAGG AGGATGATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55637
    Clone ID Related Accession (Same CDS sequence) XM_018092881.2
    Accession Version XM_018092881.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4062bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018092881.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGGGAAAA AGAGAGAAAT GTATGAACAT
    CCTGTCTTCT GCTTGGCCTC CCAAGTGATG GATTTAACCA TTCAGAATCA AAAGGACGCA
    GAAAATGTAG GCCGATTAGT AACGCCTGCC AAAAAGCTCG AAGATACAAT ACGGCTCGCA
    GAACTTGTCA TTGAAGTTCT ACAGCAGAAT GAGGAGCACC ATGCGGAGGC GTTTGCTTGG
    TGGTCGGACT TGATGGTGGA ACACGCAGAG ACCTTCCTTT CGCTTTTCGC GGTGGATATG
    GATGCTGCTT TAGAGGTGCA ACCGCCAGAC AGCTGGGACA GCTTCCCTCT TTTTCAGCTA
    ATAAATGACT TCCTTCGAAG TGACTATAAT TTATGCAATG GGAAATTCCA CAAACACCTT
    CAAGATCTCT TTGCTCCACT CGTTGTGAGA TACGTCGACC TCATGGAGTC CTCAATTGCA
    CAGTCAATTC ACAGGGGCTT TGAGCGTGAA TCATGGGAAC CAGTAAAGAG TTTAACAAGT
    AATCTACCCA ATGTGAATCT ACCAAATGTG AATCTCCCTA AAGTACCAGT TACCTTACCA
    GTTAACCTCC CACAAATGCC TAGTTTTTCT GCACCGTCAT GGATGGCAGC TATATATGAT
    TCGGACAATG GATCAGCAAC TTCGGAAGAT CTATTCTGGA AGCTTGACGC TCTGCAAACG
    TTCATTCGAG ATCTTCATTG GCCCGAGGAG GAATTCGGGA AACACCTAGA GCAACGCCTT
    AAACTTATGG CTAGCGATAT GATCGAATCG TGTGTCAAAC GAACTAGAAT TGCATTCGAA
    GTCAAACTGC AGAAAACCAG TCGATCAACA GACTTCCGAG TACCGCAATC AATATGCACC
    ATGTTTAATG TTATGGTTGA TGCCAAAGCT CAGTCAACGA AACTTTGTAG CATGGAAATG
    GGCCAAGAGC ACCAGTACCA CTCCCAAATT GACGAGCTAA TTGAAGAAAC AGTTAAAGAG
    ATGATCACAC TCTTAGTGGC AAAGTTTGTC ACAATTCTAG AAGGAGTTCT GTCCAAACTA
    TCCAGATACG ATGAAGGGAC ATTATTTTCC TCCTTTCTGT CATTTACTGT GAAAGCAGCT
    TCCAAGTATG TGGATGTACC AAAACCCGGG ATGGACCTTG CCGACGCCTA CGTGACATTT
    GTGCGCCACT CTCAGGATGT CCTGCGTGAT AAGGTCAATG AGGAGATATA TATAGAAAGG
    TTATTTGATA AAAGGCTGGA TGGTAATAGT TCTGTCATGT ACCTGAGAAT ATTGGAGCAA
    TGGTATACCA GCTCAATGAA TGTTGTTTGT ACTTGGCTGA CCGACCGTAT GGATCTGCAA
    CTTCACATTT ATCAGCTGAA AACCCTAATT AGGGTCGTCA AGAAAACCTA CCGCGATTTC
    CGATTGCAAG GCGTCTTGGA CTCCACCTTG AACAGCAAGA CCTACGACAC CATTAGAAAC
    CGCCTCACCG TGGAAGAAGC CACAGCGTCC GTGAGCGAGG GGGGAGGCCT CCAGGGAATC
    ACCATGAAGG ACAGCGACGA GGAGGATGAG GAGGATGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017948370.1
    CDS600..4661
    Translation

    Target ORF information:

    RefSeq Version XM_018092881.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092881.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGGGAAAA AGAGAGAAAT GTATGAACAT
    CCTGTCTTCT GCTTGGCCTC CCAAGTGATG GATTTAACCA TTCAGAATCA AAAGGACGCA
    GAAAATGTAG GCCGATTAGT AACGCCTGCC AAAAAGCTCG AAGATACAAT ACGGCTCGCA
    GAACTTGTCA TTGAAGTTCT ACAGCAGAAT GAGGAGCACC ATGCGGAGGC GTTTGCTTGG
    TGGTCGGACT TGATGGTGGA ACACGCAGAG ACCTTCCTTT CGCTTTTCGC GGTGGATATG
    GATGCTGCTT TAGAGGTGCA ACCGCCAGAC AGCTGGGACA GCTTCCCTCT TTTTCAGCTA
    ATAAATGACT TCCTTCGAAG TGACTATAAT TTATGCAATG GGAAATTCCA CAAACACCTT
    CAAGATCTCT TTGCTCCACT CGTTGTGAGA TACGTCGACC TCATGGAGTC CTCAATTGCA
    CAGTCAATTC ACAGGGGCTT TGAGCGTGAA TCATGGGAAC CAGTAAAGAG TTTAACAAGT
    AATCTACCCA ATGTGAATCT ACCAAATGTG AATCTCCCTA AAGTACCAGT TACCTTACCA
    GTTAACCTCC CACAAATGCC TAGTTTTTCT GCACCGTCAT GGATGGCAGC TATATATGAT
    TCGGACAATG GATCAGCAAC TTCGGAAGAT CTATTCTGGA AGCTTGACGC TCTGCAAACG
    TTCATTCGAG ATCTTCATTG GCCCGAGGAG GAATTCGGGA AACACCTAGA GCAACGCCTT
    AAACTTATGG CTAGCGATAT GATCGAATCG TGTGTCAAAC GAACTAGAAT TGCATTCGAA
    GTCAAACTGC AGAAAACCAG TCGATCAACA GACTTCCGAG TACCGCAATC AATATGCACC
    ATGTTTAATG TTATGGTTGA TGCCAAAGCT CAGTCAACGA AACTTTGTAG CATGGAAATG
    GGCCAAGAGC ACCAGTACCA CTCCCAAATT GACGAGCTAA TTGAAGAAAC AGTTAAAGAG
    ATGATCACAC TCTTAGTGGC AAAGTTTGTC ACAATTCTAG AAGGAGTTCT GTCCAAACTA
    TCCAGATACG ATGAAGGGAC ATTATTTTCC TCCTTTCTGT CATTTACTGT GAAAGCAGCT
    TCCAAGTATG TGGATGTACC AAAACCCGGG ATGGACCTTG CCGACGCCTA CGTGACATTT
    GTGCGCCACT CTCAGGATGT CCTGCGTGAT AAGGTCAATG AGGAGATATA TATAGAAAGG
    TTATTTGATA AAAGGCTGGA TGGTAATAGT TCTGTCATGT ACCTGAGAAT ATTGGAGCAA
    TGGTATACCA GCTCAATGAA TGTTGTTTGT ACTTGGCTGA CCGACCGTAT GGATCTGCAA
    CTTCACATTT ATCAGCTGAA AACCCTAATT AGGGTCGTCA AGAAAACCTA CCGCGATTTC
    CGATTGCAAG GCGTCTTGGA CTCCACCTTG AACAGCAAGA CCTACGACAC CATTAGAAAC
    CGCCTCACCG TGGAAGAAGC CACAGCGTCC GTGAGCGAGG GGGGAGGCCT CCAGGGAATC
    ACCATGAAGG ACAGCGACGA GGAGGATGAG GAGGATGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55638
    Clone ID Related Accession (Same CDS sequence) XM_018092882.2
    Accession Version XM_018092882.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4059bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018092882.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTATG CTGATAGGGC ACAAAAACAT GGCATGGATG AATTTATCTC GTCCAATCCC
    TGTAACTTTG ACCACGCCAC CCTCTTTGAG ATGGTTCAGC GACTCACACT GGATCACAGA
    CTCAACGACT CGTACTCATG TCTGGGTTGG TTCAGTCCAG GTCAGGTCTT TGTACTCGAT
    GAATATTGTG CAAGATATGG AGTCCGAGGC TGCCATAGGC ATTTGTGTTA CCTTGGTGAT
    TTACTAGAAC GAGCAGAGAA TGGGTCAATG GTCGACCCAA CTTTACTTCA CTATAGTTTT
    GCCTTCTGCG CATCCCACGT ACATGGCAAC AGGCCTGATG GCATCGGAAC TGTAACCGTG
    GAAGAAAAAG AGCGCTTTGA AGAGATCAAA GAACGTCTCC GCTTGCTTTT GGAGAATCAG
    ATCACGCATT TTAGGTACTG CTTTCCATTT GGCCGACCAG AAGGTGCTTT GAAAGCCACT
    CTGTCATTGC TGGAAAGGGT TTTAATGAAA GACATAGTAA CTCCCGTCCC TCAAGAAGAA
    GTGAAAAACG TTATCCGTAA ATGCTTAGAG CAAGCAGCCT TAGTTAACTA CACGAGATTA
    TCCGAATATG CCAAAATCGA AGGGAAAAAG AGAGAAATGT ATGAACATCC TGTCTTCTGC
    TTGGCCTCCC AAGTGATGGA TTTAACCATT CAGAATCAAA AGGACGCAGA AAATGTAGGC
    CGATTAGTAA CGCCTGCCAA AAAGCTCGAA GATACAATAC GGCTCGCAGA ACTTGTCATT
    GAAGTTCTAC AGCAGAATGA GGAGCACCAT GCGGAGGGGA AAGAGGCGTT TGCTTGGTGG
    TCGGACTTGA TGGTGGAACA CGCAGAGACC TTCCTTTCGC TTTTCGCGGT GGATATGGAT
    GCTGCTTTAG AGGTGCAACC GCCAGACAGC TGGGACAGCT TCCCTCTTTT TCAGCTAATA
    AATGACTTCC TTCGAAGTGA CTATAATTTA TGCAATGGGA AATTCCACAA ACACCTTCAA
    GATCTCTTTG CTCCACTCGT TGTGAGATAC GTCGACCTCA TGGAGTCCTC AATTGCACAG
    TCAATTCACA GGGGCTTTGA GCGTGAATCA TGGGAACCAG TAAAGAGTTT AACAAGTAAT
    CTACCCAATG TGAATCTACC AAATGTGAAT CTCCCTAAAG TACCAGTTAC CTTACCAGTT
    AACCTCCCAC AAATGCCTAG TTTTTCTGCA CCGTCATGGA TGGCAGCTAT ATATGATTCG
    GACAATGGAT CAGCAACTTC GGAAGATCTA TTCTGGAAGC TTGACGCTCT GCAAACGTTC
    ATTCGAGATC TTCATTGGCC CGAGGAGGAA TTCGGGAAAC ACCTAGAGCA ACGCCTTAAA
    CTTATGGCTA GCGATATGAT CGAATCGTGT GTCAAACGAA CTAGAATTGC ATTCGAAGTC
    AAACTGCAGA AAACCAGTCG ATCAACAGAC TTCCGAGTAC CGCAATCAAT ATGCACCATG
    TTTAATGTTA TGGTTGATGC CAAAGCTCAG TCAACGAAAC TTTGTAGCAT GGAAATGGGC
    CAAGAGCACC AGTACCACTC CCAAATTGAC GAGCTAATTG AAGAAACAGT TAAAGAGATG
    ATCACACTCT TAGTGGCAAA GTTTGTCACA ATTCTAGAAG GAGTTCTGTC CAAACTATCC
    AGATACGATG AAGGGACATT ATTTTCCTCC TTTCTGTCAT TTACTGTGAA AGCAGCTTCC
    AAGTATGTGG ATGTACCAAA ACCCGGGATG GACCTTGCCG ACGCCTACGT GACATTTGTG
    CGCCACTCTC AGGATGTCCT GCGTGATAAG GTCAATGAGG AGATATATAT AGAAAGGTTA
    TTTGATAAAA GGCTGGATGG TAATAGTTCT GTCATGTACC TGAGAATATT GGAGCAATGG
    TATACCAGCT CAATGAATGT TGTTTGTACT TGGCTGACCG ACCGTATGGA TCTGCAACTT
    CACATTTATC AGCTGAAAAC CCTAATTAGG GTCGTCAAGA AAACCTACCG CGATTTCCGA
    TTGCAAGGCG TCTTGGACTC CACCTTGAAC AGCAAGACCT ACGACACCAT TAGAAACCGC
    CTCACCGTGG AAGAAGCCAC AGCGTCCGTG AGCGAGGGGG GAGGCCTCCA GGGAATCACC
    ATGAAGGACA GCGACGAGGA GGATGAGGAG GATGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017948371.1
    CDS599..4657
    Translation

    Target ORF information:

    RefSeq Version XM_018092882.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092882.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTATG CTGATAGGGC ACAAAAACAT GGCATGGATG AATTTATCTC GTCCAATCCC
    TGTAACTTTG ACCACGCCAC CCTCTTTGAG ATGGTTCAGC GACTCACACT GGATCACAGA
    CTCAACGACT CGTACTCATG TCTGGGTTGG TTCAGTCCAG GTCAGGTCTT TGTACTCGAT
    GAATATTGTG CAAGATATGG AGTCCGAGGC TGCCATAGGC ATTTGTGTTA CCTTGGTGAT
    TTACTAGAAC GAGCAGAGAA TGGGTCAATG GTCGACCCAA CTTTACTTCA CTATAGTTTT
    GCCTTCTGCG CATCCCACGT ACATGGCAAC AGGCCTGATG GCATCGGAAC TGTAACCGTG
    GAAGAAAAAG AGCGCTTTGA AGAGATCAAA GAACGTCTCC GCTTGCTTTT GGAGAATCAG
    ATCACGCATT TTAGGTACTG CTTTCCATTT GGCCGACCAG AAGGTGCTTT GAAAGCCACT
    CTGTCATTGC TGGAAAGGGT TTTAATGAAA GACATAGTAA CTCCCGTCCC TCAAGAAGAA
    GTGAAAAACG TTATCCGTAA ATGCTTAGAG CAAGCAGCCT TAGTTAACTA CACGAGATTA
    TCCGAATATG CCAAAATCGA AGGGAAAAAG AGAGAAATGT ATGAACATCC TGTCTTCTGC
    TTGGCCTCCC AAGTGATGGA TTTAACCATT CAGAATCAAA AGGACGCAGA AAATGTAGGC
    CGATTAGTAA CGCCTGCCAA AAAGCTCGAA GATACAATAC GGCTCGCAGA ACTTGTCATT
    GAAGTTCTAC AGCAGAATGA GGAGCACCAT GCGGAGGGGA AAGAGGCGTT TGCTTGGTGG
    TCGGACTTGA TGGTGGAACA CGCAGAGACC TTCCTTTCGC TTTTCGCGGT GGATATGGAT
    GCTGCTTTAG AGGTGCAACC GCCAGACAGC TGGGACAGCT TCCCTCTTTT TCAGCTAATA
    AATGACTTCC TTCGAAGTGA CTATAATTTA TGCAATGGGA AATTCCACAA ACACCTTCAA
    GATCTCTTTG CTCCACTCGT TGTGAGATAC GTCGACCTCA TGGAGTCCTC AATTGCACAG
    TCAATTCACA GGGGCTTTGA GCGTGAATCA TGGGAACCAG TAAAGAGTTT AACAAGTAAT
    CTACCCAATG TGAATCTACC AAATGTGAAT CTCCCTAAAG TACCAGTTAC CTTACCAGTT
    AACCTCCCAC AAATGCCTAG TTTTTCTGCA CCGTCATGGA TGGCAGCTAT ATATGATTCG
    GACAATGGAT CAGCAACTTC GGAAGATCTA TTCTGGAAGC TTGACGCTCT GCAAACGTTC
    ATTCGAGATC TTCATTGGCC CGAGGAGGAA TTCGGGAAAC ACCTAGAGCA ACGCCTTAAA
    CTTATGGCTA GCGATATGAT CGAATCGTGT GTCAAACGAA CTAGAATTGC ATTCGAAGTC
    AAACTGCAGA AAACCAGTCG ATCAACAGAC TTCCGAGTAC CGCAATCAAT ATGCACCATG
    TTTAATGTTA TGGTTGATGC CAAAGCTCAG TCAACGAAAC TTTGTAGCAT GGAAATGGGC
    CAAGAGCACC AGTACCACTC CCAAATTGAC GAGCTAATTG AAGAAACAGT TAAAGAGATG
    ATCACACTCT TAGTGGCAAA GTTTGTCACA ATTCTAGAAG GAGTTCTGTC CAAACTATCC
    AGATACGATG AAGGGACATT ATTTTCCTCC TTTCTGTCAT TTACTGTGAA AGCAGCTTCC
    AAGTATGTGG ATGTACCAAA ACCCGGGATG GACCTTGCCG ACGCCTACGT GACATTTGTG
    CGCCACTCTC AGGATGTCCT GCGTGATAAG GTCAATGAGG AGATATATAT AGAAAGGTTA
    TTTGATAAAA GGCTGGATGG TAATAGTTCT GTCATGTACC TGAGAATATT GGAGCAATGG
    TATACCAGCT CAATGAATGT TGTTTGTACT TGGCTGACCG ACCGTATGGA TCTGCAACTT
    CACATTTATC AGCTGAAAAC CCTAATTAGG GTCGTCAAGA AAACCTACCG CGATTTCCGA
    TTGCAAGGCG TCTTGGACTC CACCTTGAAC AGCAAGACCT ACGACACCAT TAGAAACCGC
    CTCACCGTGG AAGAAGCCAC AGCGTCCGTG AGCGAGGGGG GAGGCCTCCA GGGAATCACC
    ATGAAGGACA GCGACGAGGA GGATGAGGAG GATGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55639
    Clone ID Related Accession (Same CDS sequence) XM_012960426.3
    Accession Version XM_012960426.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4053bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012960426.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGGGAAAA AGAGAGAAAT GTATGAACAT
    CCTGTCTTCT GCTTGGCCTC CCAAGTGATG GATTTAACCA TTCAAAATGT AGGCCGATTA
    GTAACGCCTG CCAAAAAGCT CGAAGATACA ATACGGCTCG CAGAACTTGT CATTGAAGTT
    CTACAGCAGA ATGAGGAGCA CCATGCGGAG GGGAAAGAGG CGTTTGCTTG GTGGTCGGAC
    TTGATGGTGG AACACGCAGA GACCTTCCTT TCGCTTTTCG CGGTGGATAT GGATGCTGCT
    TTAGAGGTGC AACCGCCAGA CAGCTGGGAC AGCTTCCCTC TTTTTCAGCT AATAAATGAC
    TTCCTTCGAA GTGACTATAA TTTATGCAAT GGGAAATTCC ACAAACACCT TCAAGATCTC
    TTTGCTCCAC TCGTTGTGAG ATACGTCGAC CTCATGGAGT CCTCAATTGC ACAGTCAATT
    CACAGGGGCT TTGAGCGTGA ATCATGGGAA CCAGTAAAGA GTTTAACAAG TAATCTACCC
    AATGTGAATC TACCAAATGT GAATCTCCCT AAAGTACCAG TTACCTTACC AGTTAACCTC
    CCACAAATGC CTAGTTTTTC TGCACCGTCA TGGATGGCAG CTATATATGA TTCGGACAAT
    GGATCAGCAA CTTCGGAAGA TCTATTCTGG AAGCTTGACG CTCTGCAAAC GTTCATTCGA
    GATCTTCATT GGCCCGAGGA GGAATTCGGG AAACACCTAG AGCAACGCCT TAAACTTATG
    GCTAGCGATA TGATCGAATC GTGTGTCAAA CGAACTAGAA TTGCATTCGA AGTCAAACTG
    CAGAAAACCA GTCGATCAAC AGACTTCCGA GTACCGCAAT CAATATGCAC CATGTTTAAT
    GTTATGGTTG ATGCCAAAGC TCAGTCAACG AAACTTTGTA GCATGGAAAT GGGCCAAGAG
    CACCAGTACC ACTCCCAAAT TGACGAGCTA ATTGAAGAAA CAGTTAAAGA GATGATCACA
    CTCTTAGTGG CAAAGTTTGT CACAATTCTA GAAGGAGTTC TGTCCAAACT ATCCAGATAC
    GATGAAGGGA CATTATTTTC CTCCTTTCTG TCATTTACTG TGAAAGCAGC TTCCAAGTAT
    GTGGATGTAC CAAAACCCGG GATGGACCTT GCCGACGCCT ACGTGACATT TGTGCGCCAC
    TCTCAGGATG TCCTGCGTGA TAAGGTCAAT GAGGAGATAT ATATAGAAAG GTTATTTGAT
    AAAAGGCTGG ATGGTAATAG TTCTGTCATG TACCTGAGAA TATTGGAGCA ATGGTATACC
    AGCTCAATGA ATGTTGTTTG TACTTGGCTG ACCGACCGTA TGGATCTGCA ACTTCACATT
    TATCAGCTGA AAACCCTAAT TAGGGTCGTC AAGAAAACCT ACCGCGATTT CCGATTGCAA
    GGCGTCTTGG ACTCCACCTT GAACAGCAAG ACCTACGACA CCATTAGAAA CCGCCTCACC
    GTGGAAGAAG CCACAGCGTC CGTGAGCGAG GGGGGAGGCC TCCAGGGAAT CACCATGAAG
    GACAGCGACG AGGAGGATGA GGAGGATGAT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815880.1
    CDS601..4653
    Translation

    Target ORF information:

    RefSeq Version XM_012960426.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012960426.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGGGAAAA AGAGAGAAAT GTATGAACAT
    CCTGTCTTCT GCTTGGCCTC CCAAGTGATG GATTTAACCA TTCAAAATGT AGGCCGATTA
    GTAACGCCTG CCAAAAAGCT CGAAGATACA ATACGGCTCG CAGAACTTGT CATTGAAGTT
    CTACAGCAGA ATGAGGAGCA CCATGCGGAG GGGAAAGAGG CGTTTGCTTG GTGGTCGGAC
    TTGATGGTGG AACACGCAGA GACCTTCCTT TCGCTTTTCG CGGTGGATAT GGATGCTGCT
    TTAGAGGTGC AACCGCCAGA CAGCTGGGAC AGCTTCCCTC TTTTTCAGCT AATAAATGAC
    TTCCTTCGAA GTGACTATAA TTTATGCAAT GGGAAATTCC ACAAACACCT TCAAGATCTC
    TTTGCTCCAC TCGTTGTGAG ATACGTCGAC CTCATGGAGT CCTCAATTGC ACAGTCAATT
    CACAGGGGCT TTGAGCGTGA ATCATGGGAA CCAGTAAAGA GTTTAACAAG TAATCTACCC
    AATGTGAATC TACCAAATGT GAATCTCCCT AAAGTACCAG TTACCTTACC AGTTAACCTC
    CCACAAATGC CTAGTTTTTC TGCACCGTCA TGGATGGCAG CTATATATGA TTCGGACAAT
    GGATCAGCAA CTTCGGAAGA TCTATTCTGG AAGCTTGACG CTCTGCAAAC GTTCATTCGA
    GATCTTCATT GGCCCGAGGA GGAATTCGGG AAACACCTAG AGCAACGCCT TAAACTTATG
    GCTAGCGATA TGATCGAATC GTGTGTCAAA CGAACTAGAA TTGCATTCGA AGTCAAACTG
    CAGAAAACCA GTCGATCAAC AGACTTCCGA GTACCGCAAT CAATATGCAC CATGTTTAAT
    GTTATGGTTG ATGCCAAAGC TCAGTCAACG AAACTTTGTA GCATGGAAAT GGGCCAAGAG
    CACCAGTACC ACTCCCAAAT TGACGAGCTA ATTGAAGAAA CAGTTAAAGA GATGATCACA
    CTCTTAGTGG CAAAGTTTGT CACAATTCTA GAAGGAGTTC TGTCCAAACT ATCCAGATAC
    GATGAAGGGA CATTATTTTC CTCCTTTCTG TCATTTACTG TGAAAGCAGC TTCCAAGTAT
    GTGGATGTAC CAAAACCCGG GATGGACCTT GCCGACGCCT ACGTGACATT TGTGCGCCAC
    TCTCAGGATG TCCTGCGTGA TAAGGTCAAT GAGGAGATAT ATATAGAAAG GTTATTTGAT
    AAAAGGCTGG ATGGTAATAG TTCTGTCATG TACCTGAGAA TATTGGAGCA ATGGTATACC
    AGCTCAATGA ATGTTGTTTG TACTTGGCTG ACCGACCGTA TGGATCTGCA ACTTCACATT
    TATCAGCTGA AAACCCTAAT TAGGGTCGTC AAGAAAACCT ACCGCGATTT CCGATTGCAA
    GGCGTCTTGG ACTCCACCTT GAACAGCAAG ACCTACGACA CCATTAGAAA CCGCCTCACC
    GTGGAAGAAG CCACAGCGTC CGTGAGCGAG GGGGGAGGCC TCCAGGGAAT CACCATGAAG
    GACAGCGACG AGGAGGATGA GGAGGATGAT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55640
    Clone ID Related Accession (Same CDS sequence) XM_018092883.2
    Accession Version XM_018092883.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4038bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018092883.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGGGAAAA AGAGAGAAAT GTATGAACAT
    CCTGTCTTCT GCTTGGCCTC CCAAGTGATG GATTTAACCA TTCAGAATCA AAAGGACGCA
    GAAAATGTAG GCCGATTAGT AACGCCTGCC AAAAAGCTCG AAGATACAAT ACGGCTCGCA
    GAACTTGTCA TTGAAGTTCT ACAGCAGAAT GAGGAGCACC ATGCGGAGGG GAAAGAGGCG
    TTTGCTTGGT GGTCGGACTT GATGGTGGAA CACGCAGAGA CCTTCCTTTC GCTTTTCGCG
    GTGGATATGG ATGCTGCTTT AGAGGTGCAA CCGCCAGACA GCTGGGACAG CTTCCCTCTT
    TTTCAGCTAA TAAATGACTT CCTTCGAAGT GACTATAATT TATGCAATGG GAAATTCCAC
    AAACACCTTC AAGATCTCTT TGCTCCACTC GTTGTGAGAT ACGTCGACCT CATGGAGTCC
    TCAATTGCAC AGTCAATTCA CAGGGGCTTT GAGCGTGAAT CATGGGAACC AGTAAAGAGT
    TTAACAAGTA ATCTACCCAA TGTGAATCTA CCAAATGTGA ATCTCCCTAA AGTACCAGTT
    ACCTTACCAG TTAACCTCCC ACAAATGCCT AGTTTTTCTG CACCGTCATG GATGGCAGCT
    ATATATGATT CGGACAATGG ATCAGCAACT TCGGAAGATC TATTCTGGAA GCTTGACGCT
    CTGCAAACGT TCATTCGAGA TCTTCATTGG CCCGAGGAGG AATTCGGGAA ACACCTAGAG
    CAACGCCTTA AACTTATGGC TAGCGATATG ATCGAATCGT GTGTCAAACG AACTAGAATT
    GCATTCGAAG TCAAACTGCA GAAAACCAGT CGATCAACAG ACTTCCGAGT ACCGCAATCA
    ATATGCACCA TGTTTAATGT TATGGTTGAT GCCAAAGCTC AGTCAACGAA ACTTTGTAGC
    ATGGAAATGG GCCAAGAGCA CCAGTACCAC TCCCAAATTG ACGAGCTAAT TGAAGAAACA
    GTTAAAGAGA TGATCACACT CTTAGTGGCA AAGTTTGTCA CAATTCTAGA AGGAGTTCTG
    TCCAAACTAT CCAGATACGA TGAAGGGACA TTATTTTCCT CCTTTCTGTC ATTTACTAAA
    CCCGGGATGG ACCTTGCCGA CGCCTACGTG ACATTTGTGC GCCACTCTCA GGATGTCCTG
    CGTGATAAGG TCAATGAGGA GATATATATA GAAAGGTTAT TTGATAAAAG GCTGGATGGT
    AATAGTTCTG TCATGTACCT GAGAATATTG GAGCAATGGT ATACCAGCTC AATGAATGTT
    GTTTGTACTT GGCTGACCGA CCGTATGGAT CTGCAACTTC ACATTTATCA GCTGAAAACC
    CTAATTAGGG TCGTCAAGAA AACCTACCGC GATTTCCGAT TGCAAGGCGT CTTGGACTCC
    ACCTTGAACA GCAAGACCTA CGACACCATT AGAAACCGCC TCACCGTGGA AGAAGCCACA
    GCGTCCGTGA GCGAGGGGGG AGGCCTCCAG GGAATCACCA TGAAGGACAG CGACGAGGAG
    GATGAGGAGG ATGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017948372.1
    CDS602..4639
    Translation

    Target ORF information:

    RefSeq Version XM_018092883.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092883.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGGGAAAA AGAGAGAAAT GTATGAACAT
    CCTGTCTTCT GCTTGGCCTC CCAAGTGATG GATTTAACCA TTCAGAATCA AAAGGACGCA
    GAAAATGTAG GCCGATTAGT AACGCCTGCC AAAAAGCTCG AAGATACAAT ACGGCTCGCA
    GAACTTGTCA TTGAAGTTCT ACAGCAGAAT GAGGAGCACC ATGCGGAGGG GAAAGAGGCG
    TTTGCTTGGT GGTCGGACTT GATGGTGGAA CACGCAGAGA CCTTCCTTTC GCTTTTCGCG
    GTGGATATGG ATGCTGCTTT AGAGGTGCAA CCGCCAGACA GCTGGGACAG CTTCCCTCTT
    TTTCAGCTAA TAAATGACTT CCTTCGAAGT GACTATAATT TATGCAATGG GAAATTCCAC
    AAACACCTTC AAGATCTCTT TGCTCCACTC GTTGTGAGAT ACGTCGACCT CATGGAGTCC
    TCAATTGCAC AGTCAATTCA CAGGGGCTTT GAGCGTGAAT CATGGGAACC AGTAAAGAGT
    TTAACAAGTA ATCTACCCAA TGTGAATCTA CCAAATGTGA ATCTCCCTAA AGTACCAGTT
    ACCTTACCAG TTAACCTCCC ACAAATGCCT AGTTTTTCTG CACCGTCATG GATGGCAGCT
    ATATATGATT CGGACAATGG ATCAGCAACT TCGGAAGATC TATTCTGGAA GCTTGACGCT
    CTGCAAACGT TCATTCGAGA TCTTCATTGG CCCGAGGAGG AATTCGGGAA ACACCTAGAG
    CAACGCCTTA AACTTATGGC TAGCGATATG ATCGAATCGT GTGTCAAACG AACTAGAATT
    GCATTCGAAG TCAAACTGCA GAAAACCAGT CGATCAACAG ACTTCCGAGT ACCGCAATCA
    ATATGCACCA TGTTTAATGT TATGGTTGAT GCCAAAGCTC AGTCAACGAA ACTTTGTAGC
    ATGGAAATGG GCCAAGAGCA CCAGTACCAC TCCCAAATTG ACGAGCTAAT TGAAGAAACA
    GTTAAAGAGA TGATCACACT CTTAGTGGCA AAGTTTGTCA CAATTCTAGA AGGAGTTCTG
    TCCAAACTAT CCAGATACGA TGAAGGGACA TTATTTTCCT CCTTTCTGTC ATTTACTAAA
    CCCGGGATGG ACCTTGCCGA CGCCTACGTG ACATTTGTGC GCCACTCTCA GGATGTCCTG
    CGTGATAAGG TCAATGAGGA GATATATATA GAAAGGTTAT TTGATAAAAG GCTGGATGGT
    AATAGTTCTG TCATGTACCT GAGAATATTG GAGCAATGGT ATACCAGCTC AATGAATGTT
    GTTTGTACTT GGCTGACCGA CCGTATGGAT CTGCAACTTC ACATTTATCA GCTGAAAACC
    CTAATTAGGG TCGTCAAGAA AACCTACCGC GATTTCCGAT TGCAAGGCGT CTTGGACTCC
    ACCTTGAACA GCAAGACCTA CGACACCATT AGAAACCGCC TCACCGTGGA AGAAGCCACA
    GCGTCCGTGA GCGAGGGGGG AGGCCTCCAG GGAATCACCA TGAAGGACAG CGACGAGGAG
    GATGAGGAGG ATGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55641
    Clone ID Related Accession (Same CDS sequence) XM_018092884.2
    Accession Version XM_018092884.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4035bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018092884.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGGGAAAA AGAGAGAAAT GTATGAACAT
    CCTGTCTTCT GCTTGGCCTC CCAAGTGATG GATTTAACCA TTCAGAATCA AAAGGACGCA
    GAAAATGTAG GCCGATTAGT AACGCCTGCC AAAAAGCTCG AAGATACAAT ACGGCTCGCA
    GAACTTGTCA TTGAAGTTCT ACAGCAGAAT GAGGAGCACC ATGCGGAGGG GAAAGAGGCG
    TTTGCTTGGT GGTCGGACTT GATGGTGGAA CACGCAGAGA CCTTCCTTTC GCTTTTCGCG
    GTGGATATGG ATGCTGCTTT AGAGGTGCAA CCGCCAGACA GCTGGGACAG CTTCCCTCTT
    TTTCAGCTAA TAAATGACTT CCTTCGAAGT GACTATAATT TATGCAATGG GAAATTCCAC
    AAACACCTTC AAGATCTCTT TGCTCCACTC GTTGTGAGAT ACGTCGACCT CATGGAGTCC
    TCAATTGCAC AGTCAATTCA CAGGGGCTTT GAGCGTGAAT CATGGGAACC AGTAAAGAGT
    TTAACAAGTA ATCTACCCAA TGTGAATCTA CCAAATGTGA ATCTCCCTAA AGTACCAGTT
    ACCTTACCAG TTAACCTCCC ACAAATGCCT AGTTTTTCTG CACCGTCATG GATGGCAGCT
    ATATATGATT CGGACAATGG ATCAGCAACT TCGGAAGATC TATTCTGGAA GCTTGACGCT
    CTGCAAACGT TCATTCGAGA TCTTCATTGG CCCGAGGAGG AATTCGGGAA ACACCTAGAG
    CAACGCCTTA AACTTATGGC TAGCGATATG ATCGAATCGT GTGTCAAACG AACTAGAATT
    GCATTCGAAG TCAAACTGCA GAAAACCAGT CGATCAACAG ACTTCCGAGT ACCGCAATCA
    ATATGCACCA TGTTTAATGT TATGGTTGAT GCCAAAGCTC AGTCAACGAA ACTTTGTAGC
    ATGGAAATGG GCCAAGAGCA CCAGTACCAC TCCCAAATTG ACGAGCTAAT TGAAGAAACA
    GTTAAAGAGA TGATCACACT CTTAGTGGCA AAGTTTGTCA CAATTCTAGA AGGAGTTCTG
    TCCAAACTAT CCAGATACGA TGAAGGGACA TTATTTTCCT CCTTTCTGTC ATTTACTGTG
    AAAGCAGCTT CCAAGTATGT GGATGTACCA AAACCCGGGA TGGACCTTGC CGACGCCTAC
    GTGACATTTG TGCGCCACTC TCAGGATGTC CTGCGTGATA AGAAAAGGCT GGATGGTAAT
    AGTTCTGTCA TGTACCTGAG AATATTGGAG CAATGGTATA CCAGCTCAAT GAATGTTGTT
    TGTACTTGGC TGACCGACCG TATGGATCTG CAACTTCACA TTTATCAGCT GAAAACCCTA
    ATTAGGGTCG TCAAGAAAAC CTACCGCGAT TTCCGATTGC AAGGCGTCTT GGACTCCACC
    TTGAACAGCA AGACCTACGA CACCATTAGA AACCGCCTCA CCGTGGAAGA AGCCACAGCG
    TCCGTGAGCG AGGGGGGAGG CCTCCAGGGA ATCACCATGA AGGACAGCGA CGAGGAGGAT
    GAGGAGGATG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017948373.1
    CDS601..4635
    Translation

    Target ORF information:

    RefSeq Version XM_018092884.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092884.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGGGAAAA AGAGAGAAAT GTATGAACAT
    CCTGTCTTCT GCTTGGCCTC CCAAGTGATG GATTTAACCA TTCAGAATCA AAAGGACGCA
    GAAAATGTAG GCCGATTAGT AACGCCTGCC AAAAAGCTCG AAGATACAAT ACGGCTCGCA
    GAACTTGTCA TTGAAGTTCT ACAGCAGAAT GAGGAGCACC ATGCGGAGGG GAAAGAGGCG
    TTTGCTTGGT GGTCGGACTT GATGGTGGAA CACGCAGAGA CCTTCCTTTC GCTTTTCGCG
    GTGGATATGG ATGCTGCTTT AGAGGTGCAA CCGCCAGACA GCTGGGACAG CTTCCCTCTT
    TTTCAGCTAA TAAATGACTT CCTTCGAAGT GACTATAATT TATGCAATGG GAAATTCCAC
    AAACACCTTC AAGATCTCTT TGCTCCACTC GTTGTGAGAT ACGTCGACCT CATGGAGTCC
    TCAATTGCAC AGTCAATTCA CAGGGGCTTT GAGCGTGAAT CATGGGAACC AGTAAAGAGT
    TTAACAAGTA ATCTACCCAA TGTGAATCTA CCAAATGTGA ATCTCCCTAA AGTACCAGTT
    ACCTTACCAG TTAACCTCCC ACAAATGCCT AGTTTTTCTG CACCGTCATG GATGGCAGCT
    ATATATGATT CGGACAATGG ATCAGCAACT TCGGAAGATC TATTCTGGAA GCTTGACGCT
    CTGCAAACGT TCATTCGAGA TCTTCATTGG CCCGAGGAGG AATTCGGGAA ACACCTAGAG
    CAACGCCTTA AACTTATGGC TAGCGATATG ATCGAATCGT GTGTCAAACG AACTAGAATT
    GCATTCGAAG TCAAACTGCA GAAAACCAGT CGATCAACAG ACTTCCGAGT ACCGCAATCA
    ATATGCACCA TGTTTAATGT TATGGTTGAT GCCAAAGCTC AGTCAACGAA ACTTTGTAGC
    ATGGAAATGG GCCAAGAGCA CCAGTACCAC TCCCAAATTG ACGAGCTAAT TGAAGAAACA
    GTTAAAGAGA TGATCACACT CTTAGTGGCA AAGTTTGTCA CAATTCTAGA AGGAGTTCTG
    TCCAAACTAT CCAGATACGA TGAAGGGACA TTATTTTCCT CCTTTCTGTC ATTTACTGTG
    AAAGCAGCTT CCAAGTATGT GGATGTACCA AAACCCGGGA TGGACCTTGC CGACGCCTAC
    GTGACATTTG TGCGCCACTC TCAGGATGTC CTGCGTGATA AGAAAAGGCT GGATGGTAAT
    AGTTCTGTCA TGTACCTGAG AATATTGGAG CAATGGTATA CCAGCTCAAT GAATGTTGTT
    TGTACTTGGC TGACCGACCG TATGGATCTG CAACTTCACA TTTATCAGCT GAAAACCCTA
    ATTAGGGTCG TCAAGAAAAC CTACCGCGAT TTCCGATTGC AAGGCGTCTT GGACTCCACC
    TTGAACAGCA AGACCTACGA CACCATTAGA AACCGCCTCA CCGTGGAAGA AGCCACAGCG
    TCCGTGAGCG AGGGGGGAGG CCTCCAGGGA ATCACCATGA AGGACAGCGA CGAGGAGGAT
    GAGGAGGATG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55642
    Clone ID Related Accession (Same CDS sequence) XM_018092885.2
    Accession Version XM_018092885.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4023bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018092885.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGGGAAAA AGAGAGAAAT GTATGAACAT
    CCTGTCTTCT GCTTGGCCTC CCAAGTGATG GATTTAACCA TTCAGAATCA AAAGGACGCA
    GAAAATGTAG GCCGATTAGT AACGCCTGCC AAAAAGCTCG AAGATACAAT ACGGCTCGCA
    GAACTTGTCA TTGAAGTTCT ACAGCAGAAT GAGGAGCACC ATGCGGAGGG GAAAGAGGCG
    TTTGCTTGGT GGTCGGACTT GATGGTGGAA CACGCAGAGA CCTTCCTTTC GCTTTTCGCG
    GTGGATATGG ATGCTGCTTT AGAGGTGCAA CCGCCAGACA GCTGGGACAG CTTCCCTCTT
    TTTCAGCTAA TAAATGACTT CCTTCGAAGT GACTATAATT TATGCAATGG GAAATTCCAC
    AAACACCTTC AAGATCTCTT TGCTCCACTC GTTGTGAGAT ACGTCGACCT CATGGAGTCC
    TCAATTGCAC AGTCAATTCA CAGGGGCTTT GAGCGTGAAT CATGGGAACC AGTAAAGAGT
    TTAACAAGTA ATCTACCCAA TGTGAATCTA CCAAATGTGA ATCTCCCTAA AGTACCAGTT
    ACCTTACCAG TTAACCTCCC ACAAATGCCT AGTTTTTCTG CACCGTCATG GATGGCAGCT
    ATATATGATT CGGACAATGG ATCAGCAACT TCGGAAGATC TATTCTGGAA GCTTGACGCT
    CTGCAAACGT TCATTCGAGA TCTTCATTGG CCCGAGGAGG AATTCGGGAA ACACCTAGAG
    CAACGCCTTA AACTTATGGC TAGCGATATG ATCGAATCGT GTGTCAAACG AACTAGAATT
    GCATTCGAAG TCAAACTGCA GAAAACCAGT CGATCAACAG ACTTCCGAGT ACCGCAATCA
    ATATGCACCA TGTTTAATGT TATGGTTGAT GCCAAAGCTC AGTCAACGAA ACTTTGTAGC
    ATGGAAATGG GCCAAGAGCA CCAGTACCAC TCCCAAATTG ACGAGCTAAT TGAAGAAACA
    GTTAAAGAGA TGATCACACT CTTAGTGGCA AAGTTTGTCA CAATTCTAGA AGGAGTTCTG
    TCCAAACTAT CCAGATACGA TGAAGGGACA TTATTTTCCT CCTTTCTGTC ATTTACTGTG
    AAAGCAGCTT CCAAGTATGT GGATGTACCA AAACCCGGGA TGGACCTTGC CGACGCCTAC
    GTGACATTTG TGCGCCACTC TCAGGATGTC CTGCGTGATA AGGTCAATGA GGAGATATAT
    ATAGAAAGGT TATTTGATCA ATGGTATACC AGCTCAATGA ATGTTGTTTG TACTTGGCTG
    ACCGACCGTA TGGATCTGCA ACTTCACATT TATCAGCTGA AAACCCTAAT TAGGGTCGTC
    AAGAAAACCT ACCGCGATTT CCGATTGCAA GGCGTCTTGG ACTCCACCTT GAACAGCAAG
    ACCTACGACA CCATTAGAAA CCGCCTCACC GTGGAAGAAG CCACAGCGTC CGTGAGCGAG
    GGGGGAGGCC TCCAGGGAAT CACCATGAAG GACAGCGACG AGGAGGATGA GGAGGATGAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017948374.1
    CDS599..4621
    Translation

    Target ORF information:

    RefSeq Version XM_018092885.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092885.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGGGAAAA AGAGAGAAAT GTATGAACAT
    CCTGTCTTCT GCTTGGCCTC CCAAGTGATG GATTTAACCA TTCAGAATCA AAAGGACGCA
    GAAAATGTAG GCCGATTAGT AACGCCTGCC AAAAAGCTCG AAGATACAAT ACGGCTCGCA
    GAACTTGTCA TTGAAGTTCT ACAGCAGAAT GAGGAGCACC ATGCGGAGGG GAAAGAGGCG
    TTTGCTTGGT GGTCGGACTT GATGGTGGAA CACGCAGAGA CCTTCCTTTC GCTTTTCGCG
    GTGGATATGG ATGCTGCTTT AGAGGTGCAA CCGCCAGACA GCTGGGACAG CTTCCCTCTT
    TTTCAGCTAA TAAATGACTT CCTTCGAAGT GACTATAATT TATGCAATGG GAAATTCCAC
    AAACACCTTC AAGATCTCTT TGCTCCACTC GTTGTGAGAT ACGTCGACCT CATGGAGTCC
    TCAATTGCAC AGTCAATTCA CAGGGGCTTT GAGCGTGAAT CATGGGAACC AGTAAAGAGT
    TTAACAAGTA ATCTACCCAA TGTGAATCTA CCAAATGTGA ATCTCCCTAA AGTACCAGTT
    ACCTTACCAG TTAACCTCCC ACAAATGCCT AGTTTTTCTG CACCGTCATG GATGGCAGCT
    ATATATGATT CGGACAATGG ATCAGCAACT TCGGAAGATC TATTCTGGAA GCTTGACGCT
    CTGCAAACGT TCATTCGAGA TCTTCATTGG CCCGAGGAGG AATTCGGGAA ACACCTAGAG
    CAACGCCTTA AACTTATGGC TAGCGATATG ATCGAATCGT GTGTCAAACG AACTAGAATT
    GCATTCGAAG TCAAACTGCA GAAAACCAGT CGATCAACAG ACTTCCGAGT ACCGCAATCA
    ATATGCACCA TGTTTAATGT TATGGTTGAT GCCAAAGCTC AGTCAACGAA ACTTTGTAGC
    ATGGAAATGG GCCAAGAGCA CCAGTACCAC TCCCAAATTG ACGAGCTAAT TGAAGAAACA
    GTTAAAGAGA TGATCACACT CTTAGTGGCA AAGTTTGTCA CAATTCTAGA AGGAGTTCTG
    TCCAAACTAT CCAGATACGA TGAAGGGACA TTATTTTCCT CCTTTCTGTC ATTTACTGTG
    AAAGCAGCTT CCAAGTATGT GGATGTACCA AAACCCGGGA TGGACCTTGC CGACGCCTAC
    GTGACATTTG TGCGCCACTC TCAGGATGTC CTGCGTGATA AGGTCAATGA GGAGATATAT
    ATAGAAAGGT TATTTGATCA ATGGTATACC AGCTCAATGA ATGTTGTTTG TACTTGGCTG
    ACCGACCGTA TGGATCTGCA ACTTCACATT TATCAGCTGA AAACCCTAAT TAGGGTCGTC
    AAGAAAACCT ACCGCGATTT CCGATTGCAA GGCGTCTTGG ACTCCACCTT GAACAGCAAG
    ACCTACGACA CCATTAGAAA CCGCCTCACC GTGGAAGAAG CCACAGCGTC CGTGAGCGAG
    GGGGGAGGCC TCCAGGGAAT CACCATGAAG GACAGCGACG AGGAGGATGA GGAGGATGAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55643
    Clone ID Related Accession (Same CDS sequence) XM_012960428.3
    Accession Version XM_012960428.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4002bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012960428.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGAGAATC AAAAGGACGC AGAAAATGTA
    GGCCGATTAG TAACGCCTGC CAAAAAGCTC GAAGATACAA TACGGCTCGC AGAACTTGTC
    ATTGAAGTTC TACAGCAGAA TGAGGAGCAC CATGCGGAGG GGAAAGAGGC GTTTGCTTGG
    TGGTCGGACT TGATGGTGGA ACACGCAGAG ACCTTCCTTT CGCTTTTCGC GGTGGATATG
    GATGCTGCTT TAGAGGTGCA ACCGCCAGAC AGCTGGGACA GCTTCCCTCT TTTTCAGCTA
    ATAAATGACT TCCTTCGAAG TGACTATAAT TTATGCAATG GGAAATTCCA CAAACACCTT
    CAAGATCTCT TTGCTCCACT CGTTGTGAGA TACGTCGACC TCATGGAGTC CTCAATTGCA
    CAGTCAATTC ACAGGGGCTT TGAGCGTGAA TCATGGGAAC CAGTAAAGAG TTTAACAAGT
    AATCTACCCA ATGTGAATCT ACCAAATGTG AATCTCCCTA AAGTACCAGT TACCTTACCA
    GTTAACCTCC CACAAATGCC TAGTTTTTCT GCACCGTCAT GGATGGCAGC TATATATGAT
    TCGGACAATG GATCAGCAAC TTCGGAAGAT CTATTCTGGA AGCTTGACGC TCTGCAAACG
    TTCATTCGAG ATCTTCATTG GCCCGAGGAG GAATTCGGGA AACACCTAGA GCAACGCCTT
    AAACTTATGG CTAGCGATAT GATCGAATCG TGTGTCAAAC GAACTAGAAT TGCATTCGAA
    GTCAAACTGC AGAAAACCAG TCGATCAACA GACTTCCGAG TACCGCAATC AATATGCACC
    ATGTTTAATG TTATGGTTGA TGCCAAAGCT CAGTCAACGA AACTTTGTAG CATGGAAATG
    GGCCAAGAGC ACCAGTACCA CTCCCAAATT GACGAGCTAA TTGAAGAAAC AGTTAAAGAG
    ATGATCACAC TCTTAGTGGC AAAGTTTGTC ACAATTCTAG AAGGAGTTCT GTCCAAACTA
    TCCAGATACG ATGAAGGGAC ATTATTTTCC TCCTTTCTGT CATTTACTGT GAAAGCAGCT
    TCCAAGTATG TGGATGTACC AAAACCCGGG ATGGACCTTG CCGACGCCTA CGTGACATTT
    GTGCGCCACT CTCAGGATGT CCTGCGTGAT AAGGTCAATG AGGAGATATA TATAGAAAGG
    TTATTTGATA AAAGGCTGGA TGGTAATAGT TCTGTCATGT ACCTGAGAAT ATTGGAGCAA
    TGGTATACCA GCTCAATGAA TGTTGTTTGT ACTTGGCTGA CCGACCGTAT GGATCTGCAA
    CTTCACATTT ATCAGCTGAA AACCCTAATT AGGGTCGTCA AGAAAACCTA CCGCGATTTC
    CGATTGCAAG GCGTCTTGGA CTCCACCTTG AACAGCAAGA CCTACGACAC CATTAGAAAC
    CGCCTCACCG TGGAAGAAGC CACAGCGTCC GTGAGCGAGG GGGGAGGCCT CCAGGGAATC
    ACCATGAAGG ACAGCGACGA GGAGGATGAG GAGGATGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815882.1
    CDS599..4600
    Translation

    Target ORF information:

    RefSeq Version XM_012960428.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012960428.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGAGAATC AAAAGGACGC AGAAAATGTA
    GGCCGATTAG TAACGCCTGC CAAAAAGCTC GAAGATACAA TACGGCTCGC AGAACTTGTC
    ATTGAAGTTC TACAGCAGAA TGAGGAGCAC CATGCGGAGG GGAAAGAGGC GTTTGCTTGG
    TGGTCGGACT TGATGGTGGA ACACGCAGAG ACCTTCCTTT CGCTTTTCGC GGTGGATATG
    GATGCTGCTT TAGAGGTGCA ACCGCCAGAC AGCTGGGACA GCTTCCCTCT TTTTCAGCTA
    ATAAATGACT TCCTTCGAAG TGACTATAAT TTATGCAATG GGAAATTCCA CAAACACCTT
    CAAGATCTCT TTGCTCCACT CGTTGTGAGA TACGTCGACC TCATGGAGTC CTCAATTGCA
    CAGTCAATTC ACAGGGGCTT TGAGCGTGAA TCATGGGAAC CAGTAAAGAG TTTAACAAGT
    AATCTACCCA ATGTGAATCT ACCAAATGTG AATCTCCCTA AAGTACCAGT TACCTTACCA
    GTTAACCTCC CACAAATGCC TAGTTTTTCT GCACCGTCAT GGATGGCAGC TATATATGAT
    TCGGACAATG GATCAGCAAC TTCGGAAGAT CTATTCTGGA AGCTTGACGC TCTGCAAACG
    TTCATTCGAG ATCTTCATTG GCCCGAGGAG GAATTCGGGA AACACCTAGA GCAACGCCTT
    AAACTTATGG CTAGCGATAT GATCGAATCG TGTGTCAAAC GAACTAGAAT TGCATTCGAA
    GTCAAACTGC AGAAAACCAG TCGATCAACA GACTTCCGAG TACCGCAATC AATATGCACC
    ATGTTTAATG TTATGGTTGA TGCCAAAGCT CAGTCAACGA AACTTTGTAG CATGGAAATG
    GGCCAAGAGC ACCAGTACCA CTCCCAAATT GACGAGCTAA TTGAAGAAAC AGTTAAAGAG
    ATGATCACAC TCTTAGTGGC AAAGTTTGTC ACAATTCTAG AAGGAGTTCT GTCCAAACTA
    TCCAGATACG ATGAAGGGAC ATTATTTTCC TCCTTTCTGT CATTTACTGT GAAAGCAGCT
    TCCAAGTATG TGGATGTACC AAAACCCGGG ATGGACCTTG CCGACGCCTA CGTGACATTT
    GTGCGCCACT CTCAGGATGT CCTGCGTGAT AAGGTCAATG AGGAGATATA TATAGAAAGG
    TTATTTGATA AAAGGCTGGA TGGTAATAGT TCTGTCATGT ACCTGAGAAT ATTGGAGCAA
    TGGTATACCA GCTCAATGAA TGTTGTTTGT ACTTGGCTGA CCGACCGTAT GGATCTGCAA
    CTTCACATTT ATCAGCTGAA AACCCTAATT AGGGTCGTCA AGAAAACCTA CCGCGATTTC
    CGATTGCAAG GCGTCTTGGA CTCCACCTTG AACAGCAAGA CCTACGACAC CATTAGAAAC
    CGCCTCACCG TGGAAGAAGC CACAGCGTCC GTGAGCGAGG GGGGAGGCCT CCAGGGAATC
    ACCATGAAGG ACAGCGACGA GGAGGATGAG GAGGATGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55644
    Clone ID Related Accession (Same CDS sequence) XM_018092886.2
    Accession Version XM_018092886.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4002bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018092886.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGGGAAAA AGAGAGAAAT GTATGAACAT
    CCTGTCTTCT GCTTGGCCTC CCAAGTGATG GATTTAACCA TTCAGAATCA AAAGGACGCA
    GAAAATGTAG GCCGATTAGT AACGCCTGCC AAAAAGCTCG AAGATACAAT ACGGCTCGCA
    GAACTTGTCA TTGAAGTTCT ACAGCAGAAT GAGGAGCACC ATGCGGAGGG GAAAGAGGCG
    TTTGCTTGGT GGTCGGACTT GATGGTGGAA CACGCAGAGA CCTTCCTTTC GCTTTTCGCG
    GTGGATATGG ATGCTGCTTT AGAGGTGCAA CCGCCAGACA GCTGGGACAG CTTCCCTCTT
    TTTCAGCTAA TAAATGACTT CCTTCGAAGT GACTATAATT TATGCAATGG GAAATTCCAC
    AAACACCTTC AAGATCTCTT TGCTCCACTC GTTGTGAGAT ACGTCGACCT CATGGAGTCC
    TCAATTGCAC AGTCAATTCA CAGGGGCTTT GAGCGTGAAT CATGGGAACC AGTAAAGAGT
    TTAACAAGTA ATCTACCCAA TGTGAATCTA CCAAATGTGA ATCTCCCTAA AGTACCAGTT
    ACCTTACCAG TTAACCTCCC ACAAATGCCT AGTTTTTCTG CACCGTCATG GATGGCAGCT
    ATATATGATT CGGACAATGG ATCAGCAACT TCGGAAGATC TATTCTGGAA GCTTGACGCT
    CTGCAAACGT TCATTCGAGA TCTTCATTGG CCCGAGGAGG AATTCGGGAA ACACCTAGAG
    CAACGCCTTA AACTTATGGC TAGCGATATG ATCGAATCGT GTGTCAAACG AACTAGAATT
    GCATTCGAAG TCAAACTGCA GAAAACCAGT CGATCAACAG ACTTCCGAGT ACCGCAATCA
    ATATGCACCA TGTTTAATGT TATGGTTGAT GCCAAAGCTC AGTCAACGAA ACTTTGTAGC
    ATGGAAATGG GCCAAGAGCA CCAGTACCAC TCCCAAATTG ACGAGCTAAT TGAAGAAACA
    GTTAAAGAGA TGATCACACT CTTAGTGGCA AAGTTTGTCA CAATTCTAGA AGGAGTTCTG
    TCCAAACTAT CCAGATACGA TGAAGGGACA TTATTTTCCT CCTTTCTGTC ATTTACTAAA
    CCCGGGATGG ACCTTGCCGA CGCCTACGTG ACATTTGTGC GCCACTCTCA GGATGTCCTG
    CGTGATAAGA AAAGGCTGGA TGGTAATAGT TCTGTCATGT ACCTGAGAAT ATTGGAGCAA
    TGGTATACCA GCTCAATGAA TGTTGTTTGT ACTTGGCTGA CCGACCGTAT GGATCTGCAA
    CTTCACATTT ATCAGCTGAA AACCCTAATT AGGGTCGTCA AGAAAACCTA CCGCGATTTC
    CGATTGCAAG GCGTCTTGGA CTCCACCTTG AACAGCAAGA CCTACGACAC CATTAGAAAC
    CGCCTCACCG TGGAAGAAGC CACAGCGTCC GTGAGCGAGG GGGGAGGCCT CCAGGGAATC
    ACCATGAAGG ACAGCGACGA GGAGGATGAG GAGGATGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017948375.1
    CDS602..4603
    Translation

    Target ORF information:

    RefSeq Version XM_018092886.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092886.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGGGAAAA AGAGAGAAAT GTATGAACAT
    CCTGTCTTCT GCTTGGCCTC CCAAGTGATG GATTTAACCA TTCAGAATCA AAAGGACGCA
    GAAAATGTAG GCCGATTAGT AACGCCTGCC AAAAAGCTCG AAGATACAAT ACGGCTCGCA
    GAACTTGTCA TTGAAGTTCT ACAGCAGAAT GAGGAGCACC ATGCGGAGGG GAAAGAGGCG
    TTTGCTTGGT GGTCGGACTT GATGGTGGAA CACGCAGAGA CCTTCCTTTC GCTTTTCGCG
    GTGGATATGG ATGCTGCTTT AGAGGTGCAA CCGCCAGACA GCTGGGACAG CTTCCCTCTT
    TTTCAGCTAA TAAATGACTT CCTTCGAAGT GACTATAATT TATGCAATGG GAAATTCCAC
    AAACACCTTC AAGATCTCTT TGCTCCACTC GTTGTGAGAT ACGTCGACCT CATGGAGTCC
    TCAATTGCAC AGTCAATTCA CAGGGGCTTT GAGCGTGAAT CATGGGAACC AGTAAAGAGT
    TTAACAAGTA ATCTACCCAA TGTGAATCTA CCAAATGTGA ATCTCCCTAA AGTACCAGTT
    ACCTTACCAG TTAACCTCCC ACAAATGCCT AGTTTTTCTG CACCGTCATG GATGGCAGCT
    ATATATGATT CGGACAATGG ATCAGCAACT TCGGAAGATC TATTCTGGAA GCTTGACGCT
    CTGCAAACGT TCATTCGAGA TCTTCATTGG CCCGAGGAGG AATTCGGGAA ACACCTAGAG
    CAACGCCTTA AACTTATGGC TAGCGATATG ATCGAATCGT GTGTCAAACG AACTAGAATT
    GCATTCGAAG TCAAACTGCA GAAAACCAGT CGATCAACAG ACTTCCGAGT ACCGCAATCA
    ATATGCACCA TGTTTAATGT TATGGTTGAT GCCAAAGCTC AGTCAACGAA ACTTTGTAGC
    ATGGAAATGG GCCAAGAGCA CCAGTACCAC TCCCAAATTG ACGAGCTAAT TGAAGAAACA
    GTTAAAGAGA TGATCACACT CTTAGTGGCA AAGTTTGTCA CAATTCTAGA AGGAGTTCTG
    TCCAAACTAT CCAGATACGA TGAAGGGACA TTATTTTCCT CCTTTCTGTC ATTTACTAAA
    CCCGGGATGG ACCTTGCCGA CGCCTACGTG ACATTTGTGC GCCACTCTCA GGATGTCCTG
    CGTGATAAGA AAAGGCTGGA TGGTAATAGT TCTGTCATGT ACCTGAGAAT ATTGGAGCAA
    TGGTATACCA GCTCAATGAA TGTTGTTTGT ACTTGGCTGA CCGACCGTAT GGATCTGCAA
    CTTCACATTT ATCAGCTGAA AACCCTAATT AGGGTCGTCA AGAAAACCTA CCGCGATTTC
    CGATTGCAAG GCGTCTTGGA CTCCACCTTG AACAGCAAGA CCTACGACAC CATTAGAAAC
    CGCCTCACCG TGGAAGAAGC CACAGCGTCC GTGAGCGAGG GGGGAGGCCT CCAGGGAATC
    ACCATGAAGG ACAGCGACGA GGAGGATGAG GAGGATGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55645
    Clone ID Related Accession (Same CDS sequence) XM_012960429.3
    Accession Version XM_012960429.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3990bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012960429.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTATG CTGATAGGGC ACAAAAACAT GGCATGGATG AATTTATCTC GTCCAATCCC
    TGTAACTTTG ACCACGCCAC CCTCTTTGAG ATGGTTCAGC GACTCACACT GGATCACAGA
    CTCAACGACT CGTACTCATG TCTGGGTTGG TTCAGTCCAG GTCAGGTCTT TGTACTCGAT
    GAATATTGTG CAAGATATGG AGTCCGAGGC TGCCATAGGC ATTTGTGTTA CCTTGGTGAT
    TTACTAGAAC GAGCAGAGAA TGGGTCAATG GTCGACCCAA CTTTACTTCA CTATAGTTTT
    GCCTTCTGCG CATCCCACGT ACATGGCAAC AGGCCTGATG GCATCGGAAC TGTAACCGTG
    GAAGAAAAAG AGCGCTTTGA AGAGATCAAA GAACGTCTCC GCTTGCTTTT GGAGAATCAG
    ATCACGCATT TTAGGTACTG CTTTCCATTT GGCCGACCAG AAGGTGCTTT GAAAGCCACT
    CTGTCATTGC TGGAAAGGGT TTTAATGAAA GACATAGTAA CTCCCGTCCC TCAAGAAGAA
    GTGAAAAACG TTATCCGTAA ATGCTTAGAG CAAGCAGCCT TAGTTAACTA CACGAGATTA
    TCCGAATATG CCAAAATCGA AGAGAATCAA AAGGACGCAG AAAATGTAGG CCGATTAGTA
    ACGCCTGCCA AAAAGCTCGA AGATACAATA CGGCTCGCAG AACTTGTCAT TGAAGTTCTA
    CAGCAGAATG AGGAGCACCA TGCGGAGGGG AAAGAGGCGT TTGCTTGGTG GTCGGACTTG
    ATGGTGGAAC ACGCAGAGAC CTTCCTTTCG CTTTTCGCGG TGGATATGGA TGCTGCTTTA
    GAGGTGCAAC CGCCAGACAG CTGGGACAGC TTCCCTCTTT TTCAGCTAAT AAATGACTTC
    CTTCGAAGTG ACTATAATTT ATGCAATGGG AAATTCCACA AACACCTTCA AGATCTCTTT
    GCTCCACTCG TTGTGAGATA CGTCGACCTC ATGGAGTCCT CAATTGCACA GTCAATTCAC
    AGGGGCTTTG AGCGTGAATC ATGGGAACCA GTAAAGAGTT TAACAAGTAA TCTACCCAAT
    GTGAATCTAC CAAATGTGAA TCTCCCTAAA GTACCAGTTA CCTTACCAGT TAACCTCCCA
    CAAATGCCTA GTTTTTCTGC ACCGTCATGG ATGGCAGCTA TATATGATTC GGACAATGGA
    TCAGCAACTT CGGAAGATCT ATTCTGGAAG CTTGACGCTC TGCAAACGTT CATTCGAGAT
    CTTCATTGGC CCGAGGAGGA ATTCGGGAAA CACCTAGAGC AACGCCTTAA ACTTATGGCT
    AGCGATATGA TCGAATCGTG TGTCAAACGA ACTAGAATTG CATTCGAAGT CAAACTGCAG
    AAAACCAGTC GATCAACAGA CTTCCGAGTA CCGCAATCAA TATGCACCAT GTTTAATGTT
    ATGGTTGATG CCAAAGCTCA GTCAACGAAA CTTTGTAGCA TGGAAATGGG CCAAGAGCAC
    CAGTACCACT CCCAAATTGA CGAGCTAATT GAAGAAACAG TTAAAGAGAT GATCACACTC
    TTAGTGGCAA AGTTTGTCAC AATTCTAGAA GGAGTTCTGT CCAAACTATC CAGATACGAT
    GAAGGGACAT TATTTTCCTC CTTTCTGTCA TTTACTGTGA AAGCAGCTTC CAAGTATGTG
    GATGTACCAA AACCCGGGAT GGACCTTGCC GACGCCTACG TGACATTTGT GCGCCACTCT
    CAGGATGTCC TGCGTGATAA GGTCAATGAG GAGATATATA TAGAAAGGTT ATTTGATAAA
    AGGCTGGATG GTAATAGTTC TGTCATGTAC CTGAGAATAT TGGAGCAATG GTATACCAGC
    TCAATGAATG TTGTTTGTAC TTGGCTGACC GACCGTATGG ATCTGCAACT TCACATTTAT
    CAGCTGAAAA CCCTAATTAG GGTCGTCAAG AAAACCTACC GCGATTTCCG ATTGCAAGGC
    GTCTTGGACT CCACCTTGAA CAGCAAGACC TACGACACCA TTAGAAACCG CCTCACCGTG
    GAAGAAGCCA CAGCGTCCGT GAGCGAGGGG GGAGGCCTCC AGGGAATCAC CATGAAGGAC
    AGCGACGAGG AGGATGAGGA GGATGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815883.1
    CDS597..4586
    Translation

    Target ORF information:

    RefSeq Version XM_012960429.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012960429.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTATG CTGATAGGGC ACAAAAACAT GGCATGGATG AATTTATCTC GTCCAATCCC
    TGTAACTTTG ACCACGCCAC CCTCTTTGAG ATGGTTCAGC GACTCACACT GGATCACAGA
    CTCAACGACT CGTACTCATG TCTGGGTTGG TTCAGTCCAG GTCAGGTCTT TGTACTCGAT
    GAATATTGTG CAAGATATGG AGTCCGAGGC TGCCATAGGC ATTTGTGTTA CCTTGGTGAT
    TTACTAGAAC GAGCAGAGAA TGGGTCAATG GTCGACCCAA CTTTACTTCA CTATAGTTTT
    GCCTTCTGCG CATCCCACGT ACATGGCAAC AGGCCTGATG GCATCGGAAC TGTAACCGTG
    GAAGAAAAAG AGCGCTTTGA AGAGATCAAA GAACGTCTCC GCTTGCTTTT GGAGAATCAG
    ATCACGCATT TTAGGTACTG CTTTCCATTT GGCCGACCAG AAGGTGCTTT GAAAGCCACT
    CTGTCATTGC TGGAAAGGGT TTTAATGAAA GACATAGTAA CTCCCGTCCC TCAAGAAGAA
    GTGAAAAACG TTATCCGTAA ATGCTTAGAG CAAGCAGCCT TAGTTAACTA CACGAGATTA
    TCCGAATATG CCAAAATCGA AGAGAATCAA AAGGACGCAG AAAATGTAGG CCGATTAGTA
    ACGCCTGCCA AAAAGCTCGA AGATACAATA CGGCTCGCAG AACTTGTCAT TGAAGTTCTA
    CAGCAGAATG AGGAGCACCA TGCGGAGGGG AAAGAGGCGT TTGCTTGGTG GTCGGACTTG
    ATGGTGGAAC ACGCAGAGAC CTTCCTTTCG CTTTTCGCGG TGGATATGGA TGCTGCTTTA
    GAGGTGCAAC CGCCAGACAG CTGGGACAGC TTCCCTCTTT TTCAGCTAAT AAATGACTTC
    CTTCGAAGTG ACTATAATTT ATGCAATGGG AAATTCCACA AACACCTTCA AGATCTCTTT
    GCTCCACTCG TTGTGAGATA CGTCGACCTC ATGGAGTCCT CAATTGCACA GTCAATTCAC
    AGGGGCTTTG AGCGTGAATC ATGGGAACCA GTAAAGAGTT TAACAAGTAA TCTACCCAAT
    GTGAATCTAC CAAATGTGAA TCTCCCTAAA GTACCAGTTA CCTTACCAGT TAACCTCCCA
    CAAATGCCTA GTTTTTCTGC ACCGTCATGG ATGGCAGCTA TATATGATTC GGACAATGGA
    TCAGCAACTT CGGAAGATCT ATTCTGGAAG CTTGACGCTC TGCAAACGTT CATTCGAGAT
    CTTCATTGGC CCGAGGAGGA ATTCGGGAAA CACCTAGAGC AACGCCTTAA ACTTATGGCT
    AGCGATATGA TCGAATCGTG TGTCAAACGA ACTAGAATTG CATTCGAAGT CAAACTGCAG
    AAAACCAGTC GATCAACAGA CTTCCGAGTA CCGCAATCAA TATGCACCAT GTTTAATGTT
    ATGGTTGATG CCAAAGCTCA GTCAACGAAA CTTTGTAGCA TGGAAATGGG CCAAGAGCAC
    CAGTACCACT CCCAAATTGA CGAGCTAATT GAAGAAACAG TTAAAGAGAT GATCACACTC
    TTAGTGGCAA AGTTTGTCAC AATTCTAGAA GGAGTTCTGT CCAAACTATC CAGATACGAT
    GAAGGGACAT TATTTTCCTC CTTTCTGTCA TTTACTGTGA AAGCAGCTTC CAAGTATGTG
    GATGTACCAA AACCCGGGAT GGACCTTGCC GACGCCTACG TGACATTTGT GCGCCACTCT
    CAGGATGTCC TGCGTGATAA GGTCAATGAG GAGATATATA TAGAAAGGTT ATTTGATAAA
    AGGCTGGATG GTAATAGTTC TGTCATGTAC CTGAGAATAT TGGAGCAATG GTATACCAGC
    TCAATGAATG TTGTTTGTAC TTGGCTGACC GACCGTATGG ATCTGCAACT TCACATTTAT
    CAGCTGAAAA CCCTAATTAG GGTCGTCAAG AAAACCTACC GCGATTTCCG ATTGCAAGGC
    GTCTTGGACT CCACCTTGAA CAGCAAGACC TACGACACCA TTAGAAACCG CCTCACCGTG
    GAAGAAGCCA CAGCGTCCGT GAGCGAGGGG GGAGGCCTCC AGGGAATCAC CATGAAGGAC
    AGCGACGAGG AGGATGAGGA GGATGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55646
    Clone ID Related Accession (Same CDS sequence) XM_012960430.3
    Accession Version XM_012960430.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3984bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012960430.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTATG CTGATAGGGC ACAAAAACAT GGCATGGATG AATTTATCTC GTCCAATCCC
    TGTAACTTTG ACCACGCCAC CCTCTTTGAG ATGGTTCAGC GACTCACACT GGATCACAGA
    CTCAACGACT CGTACTCATG TCTGGGTTGG TTCAGTCCAG GTCAGGTCTT TGTACTCGAT
    GAATATTGTG CAAGATATGG AGTCCGAGGC TGCCATAGGC ATTTGTGTTA CCTTGGTGAT
    TTACTAGAAC GAGCAGAGAA TGGGTCAATG GTCGACCCAA CTTTACTTCA CTATAGTTTT
    GCCTTCTGCG CATCCCACGT ACATGGCAAC AGGCCTGATG GCATCGGAAC TGTAACCGTG
    GAAGAAAAAG AGCGCTTTGA AGAGATCAAA GAACGTCTCC GCTTGCTTTT GGAGAATCAG
    ATCACGCATT TTAGGTACTG CTTTCCATTT GGCCGACCAG AAGGTGCTTT GAAAGCCACT
    CTGTCATTGC TGGAAAGGGT TTTAATGAAA GACATAGTAA CTCCCGTCCC TCAAGAAGAA
    GTGAAAAACG TTATCCGTAA ATGCTTAGAG CAAGCAGCCT TAGTTAACTA CACGAGATTA
    TCCGAATATG CCAAAATCGA AGGGAAAAAG AGAGAAATGT ATGAACATCC TGTCTTCTGC
    TTGGCCTCCC AAGTGATGGA TTTAACCATT CAAAATGTAG GCCGATTAGT AACGCCTGCC
    AAAAAGCTCG AAGATACAAT ACGGCTCGCA GAACTTGTCA TTGAAGTTCT ACAGCAGAAT
    GAGGAGCACC ATGCGGAGGC GTTTGCTTGG TGGTCGGACT TGATGGTGGA ACACGCAGAG
    ACCTTCCTTT CGCTTTTCGC GGTGGATATG GATGCTGCTT TAGAGGTGCA ACCGCCAGAC
    AGCTGGGACA GCTTCCCTCT TTTTCAGCTA ATAAATGACT TCCTTCGAAG TGACTATAAT
    TTATGCAATG GGAAATTCCA CAAACACCTT CAAGATCTCT TTGCTCCACT CGTTGTGAGA
    TACGTCGACC TCATGGAGTC CTCAATTGCA CAGTCAATTC ACAGGGGCTT TGAGCGTGAA
    TCATGGGAAC CAGTAAAGAG TTTAACAAGT AATCTACCCA ATGTGAATCT ACCAAATGTG
    AATCTCCCTA AAGTACCAGT TACCTTACCA GTTAACCTCC CACAAATGCC TAGTTTTTCT
    GCACCGTCAT GGATGGCAGC TATATATGAT TCGGACAATG GATCAGCAAC TTCGGAAGAT
    CTATTCTGGA AGCTTGACGC TCTGCAAACG TTCATTCGAG ATCTTCATTG GCCCGAGGAG
    GAATTCGGGA AACACCTAGA GCAACGCCTT AAACTTATGG CTAGCGATAT GATCGAATCG
    TGTGTCAAAC GAACTAGAAT TGCATTCGAA GTCAAACTGC AGAAAACCAG TCGATCAACA
    GACTTCCGAG TACCGCAATC AATATGCACC ATGTTTAATG TTATGGTTGA TGCCAAAGCT
    CAGTCAACGA AACTTTGTAG CATGGAAATG GGCCAAGAGC ACCAGTACCA CTCCCAAATT
    GACGAGCTAA TTGAAGAAAC AGTTAAAGAG ATGATCACAC TCTTAGTGGC AAAGTTTGTC
    ACAATTCTAG AAGGAGTTCT GTCCAAACTA TCCAGATACG ATGAAGGGAC ATTATTTTCC
    TCCTTTCTGT CATTTACTGT GAAAGCAGCT TCCAAGTATG TGGATGTACC AAAACCCGGG
    ATGGACCTTG CCGACGCCTA CGTGACATTT GTGCGCCACT CTCAGGATGT CCTGCGTGAT
    AAGGTCAATG AGGAGATATA TATAGAAAGG TTATTTGATC AATGGTATAC CAGCTCAATG
    AATGTTGTTT GTACTTGGCT GACCGACCGT ATGGATCTGC AACTTCACAT TTATCAGCTG
    AAAACCCTAA TTAGGGTCGT CAAGAAAACC TACCGCGATT TCCGATTGCA AGGCGTCTTG
    GACTCCACCT TGAACAGCAA GACCTACGAC ACCATTAGAA ACCGCCTCAC CGTGGAAGAA
    GCCACAGCGT CCGTGAGCGA GGGGGGAGGC CTCCAGGGAA TCACCATGAA GGACAGCGAC
    GAGGAGGATG AGGAGGATGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815884.1
    CDS595..4578
    Translation

    Target ORF information:

    RefSeq Version XM_012960430.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012960430.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTATG CTGATAGGGC ACAAAAACAT GGCATGGATG AATTTATCTC GTCCAATCCC
    TGTAACTTTG ACCACGCCAC CCTCTTTGAG ATGGTTCAGC GACTCACACT GGATCACAGA
    CTCAACGACT CGTACTCATG TCTGGGTTGG TTCAGTCCAG GTCAGGTCTT TGTACTCGAT
    GAATATTGTG CAAGATATGG AGTCCGAGGC TGCCATAGGC ATTTGTGTTA CCTTGGTGAT
    TTACTAGAAC GAGCAGAGAA TGGGTCAATG GTCGACCCAA CTTTACTTCA CTATAGTTTT
    GCCTTCTGCG CATCCCACGT ACATGGCAAC AGGCCTGATG GCATCGGAAC TGTAACCGTG
    GAAGAAAAAG AGCGCTTTGA AGAGATCAAA GAACGTCTCC GCTTGCTTTT GGAGAATCAG
    ATCACGCATT TTAGGTACTG CTTTCCATTT GGCCGACCAG AAGGTGCTTT GAAAGCCACT
    CTGTCATTGC TGGAAAGGGT TTTAATGAAA GACATAGTAA CTCCCGTCCC TCAAGAAGAA
    GTGAAAAACG TTATCCGTAA ATGCTTAGAG CAAGCAGCCT TAGTTAACTA CACGAGATTA
    TCCGAATATG CCAAAATCGA AGGGAAAAAG AGAGAAATGT ATGAACATCC TGTCTTCTGC
    TTGGCCTCCC AAGTGATGGA TTTAACCATT CAAAATGTAG GCCGATTAGT AACGCCTGCC
    AAAAAGCTCG AAGATACAAT ACGGCTCGCA GAACTTGTCA TTGAAGTTCT ACAGCAGAAT
    GAGGAGCACC ATGCGGAGGC GTTTGCTTGG TGGTCGGACT TGATGGTGGA ACACGCAGAG
    ACCTTCCTTT CGCTTTTCGC GGTGGATATG GATGCTGCTT TAGAGGTGCA ACCGCCAGAC
    AGCTGGGACA GCTTCCCTCT TTTTCAGCTA ATAAATGACT TCCTTCGAAG TGACTATAAT
    TTATGCAATG GGAAATTCCA CAAACACCTT CAAGATCTCT TTGCTCCACT CGTTGTGAGA
    TACGTCGACC TCATGGAGTC CTCAATTGCA CAGTCAATTC ACAGGGGCTT TGAGCGTGAA
    TCATGGGAAC CAGTAAAGAG TTTAACAAGT AATCTACCCA ATGTGAATCT ACCAAATGTG
    AATCTCCCTA AAGTACCAGT TACCTTACCA GTTAACCTCC CACAAATGCC TAGTTTTTCT
    GCACCGTCAT GGATGGCAGC TATATATGAT TCGGACAATG GATCAGCAAC TTCGGAAGAT
    CTATTCTGGA AGCTTGACGC TCTGCAAACG TTCATTCGAG ATCTTCATTG GCCCGAGGAG
    GAATTCGGGA AACACCTAGA GCAACGCCTT AAACTTATGG CTAGCGATAT GATCGAATCG
    TGTGTCAAAC GAACTAGAAT TGCATTCGAA GTCAAACTGC AGAAAACCAG TCGATCAACA
    GACTTCCGAG TACCGCAATC AATATGCACC ATGTTTAATG TTATGGTTGA TGCCAAAGCT
    CAGTCAACGA AACTTTGTAG CATGGAAATG GGCCAAGAGC ACCAGTACCA CTCCCAAATT
    GACGAGCTAA TTGAAGAAAC AGTTAAAGAG ATGATCACAC TCTTAGTGGC AAAGTTTGTC
    ACAATTCTAG AAGGAGTTCT GTCCAAACTA TCCAGATACG ATGAAGGGAC ATTATTTTCC
    TCCTTTCTGT CATTTACTGT GAAAGCAGCT TCCAAGTATG TGGATGTACC AAAACCCGGG
    ATGGACCTTG CCGACGCCTA CGTGACATTT GTGCGCCACT CTCAGGATGT CCTGCGTGAT
    AAGGTCAATG AGGAGATATA TATAGAAAGG TTATTTGATC AATGGTATAC CAGCTCAATG
    AATGTTGTTT GTACTTGGCT GACCGACCGT ATGGATCTGC AACTTCACAT TTATCAGCTG
    AAAACCCTAA TTAGGGTCGT CAAGAAAACC TACCGCGATT TCCGATTGCA AGGCGTCTTG
    GACTCCACCT TGAACAGCAA GACCTACGAC ACCATTAGAA ACCGCCTCAC CGTGGAAGAA
    GCCACAGCGT CCGTGAGCGA GGGGGGAGGC CTCCAGGGAA TCACCATGAA GGACAGCGAC
    GAGGAGGATG AGGAGGATGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55647
    Clone ID Related Accession (Same CDS sequence) XM_012960431.3
    Accession Version XM_012960431.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3984bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012960431.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGAAAATG TAGGCCGATT AGTAACGCCT
    GCCAAAAAGC TCGAAGATAC AATACGGCTC GCAGAACTTG TCATTGAAGT TCTACAGCAG
    AATGAGGAGC ACCATGCGGA GGGGAAAGAG GCGTTTGCTT GGTGGTCGGA CTTGATGGTG
    GAACACGCAG AGACCTTCCT TTCGCTTTTC GCGGTGGATA TGGATGCTGC TTTAGAGGTG
    CAACCGCCAG ACAGCTGGGA CAGCTTCCCT CTTTTTCAGC TAATAAATGA CTTCCTTCGA
    AGTGACTATA ATTTATGCAA TGGGAAATTC CACAAACACC TTCAAGATCT CTTTGCTCCA
    CTCGTTGTGA GATACGTCGA CCTCATGGAG TCCTCAATTG CACAGTCAAT TCACAGGGGC
    TTTGAGCGTG AATCATGGGA ACCAGTAAAG AGTTTAACAA GTAATCTACC CAATGTGAAT
    CTACCAAATG TGAATCTCCC TAAAGTACCA GTTACCTTAC CAGTTAACCT CCCACAAATG
    CCTAGTTTTT CTGCACCGTC ATGGATGGCA GCTATATATG ATTCGGACAA TGGATCAGCA
    ACTTCGGAAG ATCTATTCTG GAAGCTTGAC GCTCTGCAAA CGTTCATTCG AGATCTTCAT
    TGGCCCGAGG AGGAATTCGG GAAACACCTA GAGCAACGCC TTAAACTTAT GGCTAGCGAT
    ATGATCGAAT CGTGTGTCAA ACGAACTAGA ATTGCATTCG AAGTCAAACT GCAGAAAACC
    AGTCGATCAA CAGACTTCCG AGTACCGCAA TCAATATGCA CCATGTTTAA TGTTATGGTT
    GATGCCAAAG CTCAGTCAAC GAAACTTTGT AGCATGGAAA TGGGCCAAGA GCACCAGTAC
    CACTCCCAAA TTGACGAGCT AATTGAAGAA ACAGTTAAAG AGATGATCAC ACTCTTAGTG
    GCAAAGTTTG TCACAATTCT AGAAGGAGTT CTGTCCAAAC TATCCAGATA CGATGAAGGG
    ACATTATTTT CCTCCTTTCT GTCATTTACT GTGAAAGCAG CTTCCAAGTA TGTGGATGTA
    CCAAAACCCG GGATGGACCT TGCCGACGCC TACGTGACAT TTGTGCGCCA CTCTCAGGAT
    GTCCTGCGTG ATAAGGTCAA TGAGGAGATA TATATAGAAA GGTTATTTGA TAAAAGGCTG
    GATGGTAATA GTTCTGTCAT GTACCTGAGA ATATTGGAGC AATGGTATAC CAGCTCAATG
    AATGTTGTTT GTACTTGGCT GACCGACCGT ATGGATCTGC AACTTCACAT TTATCAGCTG
    AAAACCCTAA TTAGGGTCGT CAAGAAAACC TACCGCGATT TCCGATTGCA AGGCGTCTTG
    GACTCCACCT TGAACAGCAA GACCTACGAC ACCATTAGAA ACCGCCTCAC CGTGGAAGAA
    GCCACAGCGT CCGTGAGCGA GGGGGGAGGC CTCCAGGGAA TCACCATGAA GGACAGCGAC
    GAGGAGGATG AGGAGGATGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815885.1
    CDS599..4582
    Translation

    Target ORF information:

    RefSeq Version XM_012960431.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012960431.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGAAAATG TAGGCCGATT AGTAACGCCT
    GCCAAAAAGC TCGAAGATAC AATACGGCTC GCAGAACTTG TCATTGAAGT TCTACAGCAG
    AATGAGGAGC ACCATGCGGA GGGGAAAGAG GCGTTTGCTT GGTGGTCGGA CTTGATGGTG
    GAACACGCAG AGACCTTCCT TTCGCTTTTC GCGGTGGATA TGGATGCTGC TTTAGAGGTG
    CAACCGCCAG ACAGCTGGGA CAGCTTCCCT CTTTTTCAGC TAATAAATGA CTTCCTTCGA
    AGTGACTATA ATTTATGCAA TGGGAAATTC CACAAACACC TTCAAGATCT CTTTGCTCCA
    CTCGTTGTGA GATACGTCGA CCTCATGGAG TCCTCAATTG CACAGTCAAT TCACAGGGGC
    TTTGAGCGTG AATCATGGGA ACCAGTAAAG AGTTTAACAA GTAATCTACC CAATGTGAAT
    CTACCAAATG TGAATCTCCC TAAAGTACCA GTTACCTTAC CAGTTAACCT CCCACAAATG
    CCTAGTTTTT CTGCACCGTC ATGGATGGCA GCTATATATG ATTCGGACAA TGGATCAGCA
    ACTTCGGAAG ATCTATTCTG GAAGCTTGAC GCTCTGCAAA CGTTCATTCG AGATCTTCAT
    TGGCCCGAGG AGGAATTCGG GAAACACCTA GAGCAACGCC TTAAACTTAT GGCTAGCGAT
    ATGATCGAAT CGTGTGTCAA ACGAACTAGA ATTGCATTCG AAGTCAAACT GCAGAAAACC
    AGTCGATCAA CAGACTTCCG AGTACCGCAA TCAATATGCA CCATGTTTAA TGTTATGGTT
    GATGCCAAAG CTCAGTCAAC GAAACTTTGT AGCATGGAAA TGGGCCAAGA GCACCAGTAC
    CACTCCCAAA TTGACGAGCT AATTGAAGAA ACAGTTAAAG AGATGATCAC ACTCTTAGTG
    GCAAAGTTTG TCACAATTCT AGAAGGAGTT CTGTCCAAAC TATCCAGATA CGATGAAGGG
    ACATTATTTT CCTCCTTTCT GTCATTTACT GTGAAAGCAG CTTCCAAGTA TGTGGATGTA
    CCAAAACCCG GGATGGACCT TGCCGACGCC TACGTGACAT TTGTGCGCCA CTCTCAGGAT
    GTCCTGCGTG ATAAGGTCAA TGAGGAGATA TATATAGAAA GGTTATTTGA TAAAAGGCTG
    GATGGTAATA GTTCTGTCAT GTACCTGAGA ATATTGGAGC AATGGTATAC CAGCTCAATG
    AATGTTGTTT GTACTTGGCT GACCGACCGT ATGGATCTGC AACTTCACAT TTATCAGCTG
    AAAACCCTAA TTAGGGTCGT CAAGAAAACC TACCGCGATT TCCGATTGCA AGGCGTCTTG
    GACTCCACCT TGAACAGCAA GACCTACGAC ACCATTAGAA ACCGCCTCAC CGTGGAAGAA
    GCCACAGCGT CCGTGAGCGA GGGGGGAGGC CTCCAGGGAA TCACCATGAA GGACAGCGAC
    GAGGAGGATG AGGAGGATGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55648
    Clone ID Related Accession (Same CDS sequence) XM_012960432.3
    Accession Version XM_012960432.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3972bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012960432.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTATG CTGATAGGGC ACAAAAACAT GGCATGGATG AATTTATCTC GTCCAATCCC
    TGTAACTTTG ACCACGCCAC CCTCTTTGAG ATGGTTCAGC GACTCACACT GGATCACAGA
    CTCAACGACT CGTACTCATG TCTGGGTTGG TTCAGTCCAG GTCAGGTCTT TGTACTCGAT
    GAATATTGTG CAAGATATGG AGTCCGAGGC TGCCATAGGC ATTTGTGTTA CCTTGGTGAT
    TTACTAGAAC GAGCAGAGAA TGGGTCAATG GTCGACCCAA CTTTACTTCA CTATAGTTTT
    GCCTTCTGCG CATCCCACGT ACATGGCAAC AGGCCTGATG GCATCGGAAC TGTAACCGTG
    GAAGAAAAAG AGCGCTTTGA AGAGATCAAA GAACGTCTCC GCTTGCTTTT GGAGAATCAG
    ATCACGCATT TTAGGTACTG CTTTCCATTT GGCCGACCAG AAGGTGCTTT GAAAGCCACT
    CTGTCATTGC TGGAAAGGGT TTTAATGAAA GACATAGTAA CTCCCGTCCC TCAAGAAGAA
    GTGAAAAACG TTATCCGTAA ATGCTTAGAG CAAGCAGCCT TAGTTAACTA CACGAGATTA
    TCCGAATATG CCAAAATCGA AGAAAATGTA GGCCGATTAG TAACGCCTGC CAAAAAGCTC
    GAAGATACAA TACGGCTCGC AGAACTTGTC ATTGAAGTTC TACAGCAGAA TGAGGAGCAC
    CATGCGGAGG GGAAAGAGGC GTTTGCTTGG TGGTCGGACT TGATGGTGGA ACACGCAGAG
    ACCTTCCTTT CGCTTTTCGC GGTGGATATG GATGCTGCTT TAGAGGTGCA ACCGCCAGAC
    AGCTGGGACA GCTTCCCTCT TTTTCAGCTA ATAAATGACT TCCTTCGAAG TGACTATAAT
    TTATGCAATG GGAAATTCCA CAAACACCTT CAAGATCTCT TTGCTCCACT CGTTGTGAGA
    TACGTCGACC TCATGGAGTC CTCAATTGCA CAGTCAATTC ACAGGGGCTT TGAGCGTGAA
    TCATGGGAAC CAGTAAAGAG TTTAACAAGT AATCTACCCA ATGTGAATCT ACCAAATGTG
    AATCTCCCTA AAGTACCAGT TACCTTACCA GTTAACCTCC CACAAATGCC TAGTTTTTCT
    GCACCGTCAT GGATGGCAGC TATATATGAT TCGGACAATG GATCAGCAAC TTCGGAAGAT
    CTATTCTGGA AGCTTGACGC TCTGCAAACG TTCATTCGAG ATCTTCATTG GCCCGAGGAG
    GAATTCGGGA AACACCTAGA GCAACGCCTT AAACTTATGG CTAGCGATAT GATCGAATCG
    TGTGTCAAAC GAACTAGAAT TGCATTCGAA GTCAAACTGC AGAAAACCAG TCGATCAACA
    GACTTCCGAG TACCGCAATC AATATGCACC ATGTTTAATG TTATGGTTGA TGCCAAAGCT
    CAGTCAACGA AACTTTGTAG CATGGAAATG GGCCAAGAGC ACCAGTACCA CTCCCAAATT
    GACGAGCTAA TTGAAGAAAC AGTTAAAGAG ATGATCACAC TCTTAGTGGC AAAGTTTGTC
    ACAATTCTAG AAGGAGTTCT GTCCAAACTA TCCAGATACG ATGAAGGGAC ATTATTTTCC
    TCCTTTCTGT CATTTACTGT GAAAGCAGCT TCCAAGTATG TGGATGTACC AAAACCCGGG
    ATGGACCTTG CCGACGCCTA CGTGACATTT GTGCGCCACT CTCAGGATGT CCTGCGTGAT
    AAGGTCAATG AGGAGATATA TATAGAAAGG TTATTTGATA AAAGGCTGGA TGGTAATAGT
    TCTGTCATGT ACCTGAGAAT ATTGGAGCAA TGGTATACCA GCTCAATGAA TGTTGTTTGT
    ACTTGGCTGA CCGACCGTAT GGATCTGCAA CTTCACATTT ATCAGCTGAA AACCCTAATT
    AGGGTCGTCA AGAAAACCTA CCGCGATTTC CGATTGCAAG GCGTCTTGGA CTCCACCTTG
    AACAGCAAGA CCTACGACAC CATTAGAAAC CGCCTCACCG TGGAAGAAGC CACAGCGTCC
    GTGAGCGAGG GGGGAGGCCT CCAGGGAATC ACCATGAAGG ACAGCGACGA GGAGGATGAG
    GAGGATGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815886.1
    CDS597..4568
    Translation

    Target ORF information:

    RefSeq Version XM_012960432.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012960432.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTATG CTGATAGGGC ACAAAAACAT GGCATGGATG AATTTATCTC GTCCAATCCC
    TGTAACTTTG ACCACGCCAC CCTCTTTGAG ATGGTTCAGC GACTCACACT GGATCACAGA
    CTCAACGACT CGTACTCATG TCTGGGTTGG TTCAGTCCAG GTCAGGTCTT TGTACTCGAT
    GAATATTGTG CAAGATATGG AGTCCGAGGC TGCCATAGGC ATTTGTGTTA CCTTGGTGAT
    TTACTAGAAC GAGCAGAGAA TGGGTCAATG GTCGACCCAA CTTTACTTCA CTATAGTTTT
    GCCTTCTGCG CATCCCACGT ACATGGCAAC AGGCCTGATG GCATCGGAAC TGTAACCGTG
    GAAGAAAAAG AGCGCTTTGA AGAGATCAAA GAACGTCTCC GCTTGCTTTT GGAGAATCAG
    ATCACGCATT TTAGGTACTG CTTTCCATTT GGCCGACCAG AAGGTGCTTT GAAAGCCACT
    CTGTCATTGC TGGAAAGGGT TTTAATGAAA GACATAGTAA CTCCCGTCCC TCAAGAAGAA
    GTGAAAAACG TTATCCGTAA ATGCTTAGAG CAAGCAGCCT TAGTTAACTA CACGAGATTA
    TCCGAATATG CCAAAATCGA AGAAAATGTA GGCCGATTAG TAACGCCTGC CAAAAAGCTC
    GAAGATACAA TACGGCTCGC AGAACTTGTC ATTGAAGTTC TACAGCAGAA TGAGGAGCAC
    CATGCGGAGG GGAAAGAGGC GTTTGCTTGG TGGTCGGACT TGATGGTGGA ACACGCAGAG
    ACCTTCCTTT CGCTTTTCGC GGTGGATATG GATGCTGCTT TAGAGGTGCA ACCGCCAGAC
    AGCTGGGACA GCTTCCCTCT TTTTCAGCTA ATAAATGACT TCCTTCGAAG TGACTATAAT
    TTATGCAATG GGAAATTCCA CAAACACCTT CAAGATCTCT TTGCTCCACT CGTTGTGAGA
    TACGTCGACC TCATGGAGTC CTCAATTGCA CAGTCAATTC ACAGGGGCTT TGAGCGTGAA
    TCATGGGAAC CAGTAAAGAG TTTAACAAGT AATCTACCCA ATGTGAATCT ACCAAATGTG
    AATCTCCCTA AAGTACCAGT TACCTTACCA GTTAACCTCC CACAAATGCC TAGTTTTTCT
    GCACCGTCAT GGATGGCAGC TATATATGAT TCGGACAATG GATCAGCAAC TTCGGAAGAT
    CTATTCTGGA AGCTTGACGC TCTGCAAACG TTCATTCGAG ATCTTCATTG GCCCGAGGAG
    GAATTCGGGA AACACCTAGA GCAACGCCTT AAACTTATGG CTAGCGATAT GATCGAATCG
    TGTGTCAAAC GAACTAGAAT TGCATTCGAA GTCAAACTGC AGAAAACCAG TCGATCAACA
    GACTTCCGAG TACCGCAATC AATATGCACC ATGTTTAATG TTATGGTTGA TGCCAAAGCT
    CAGTCAACGA AACTTTGTAG CATGGAAATG GGCCAAGAGC ACCAGTACCA CTCCCAAATT
    GACGAGCTAA TTGAAGAAAC AGTTAAAGAG ATGATCACAC TCTTAGTGGC AAAGTTTGTC
    ACAATTCTAG AAGGAGTTCT GTCCAAACTA TCCAGATACG ATGAAGGGAC ATTATTTTCC
    TCCTTTCTGT CATTTACTGT GAAAGCAGCT TCCAAGTATG TGGATGTACC AAAACCCGGG
    ATGGACCTTG CCGACGCCTA CGTGACATTT GTGCGCCACT CTCAGGATGT CCTGCGTGAT
    AAGGTCAATG AGGAGATATA TATAGAAAGG TTATTTGATA AAAGGCTGGA TGGTAATAGT
    TCTGTCATGT ACCTGAGAAT ATTGGAGCAA TGGTATACCA GCTCAATGAA TGTTGTTTGT
    ACTTGGCTGA CCGACCGTAT GGATCTGCAA CTTCACATTT ATCAGCTGAA AACCCTAATT
    AGGGTCGTCA AGAAAACCTA CCGCGATTTC CGATTGCAAG GCGTCTTGGA CTCCACCTTG
    AACAGCAAGA CCTACGACAC CATTAGAAAC CGCCTCACCG TGGAAGAAGC CACAGCGTCC
    GTGAGCGAGG GGGGAGGCCT CCAGGGAATC ACCATGAAGG ACAGCGACGA GGAGGATGAG
    GAGGATGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55649
    Clone ID Related Accession (Same CDS sequence) XM_012960440.3
    Accession Version XM_012960440.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3942bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012960440.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTATG CTGATAGGGC ACAAAAACAT GGCATGGATG AATTTATCTC GTCCAATCCC
    TGTAACTTTG ACCACGCCAC CCTCTTTGAG ATGGTTCAGC GACTCACACT GGATCACAGA
    CTCAACGACT CGTACTCATG TCTGGGTTGG TTCAGTCCAG GTCAGGTCTT TGTACTCGAT
    GAATATTGTG CAAGATATGG AGTCCGAGGC TGCCATAGGC ATTTGTGTTA CCTTGGTGAT
    TTACTAGAAC GAGCAGAGAA TGGGTCAATG GTCGACCCAA CTTTACTTCA CTATAGTTTT
    GCCTTCTGCG CATCCCACGT ACATGGCAAC AGGCCTGATG GCATCGGAAC TGTAACCGTG
    GAAGAAAAAG AGCGCTTTGA AGAGATCAAA GAACGTCTCC GCTTGCTTTT GGAGAATCAG
    ATCACGCATT TTAGGTACTG CTTTCCATTT GGCCGACCAG AAGGTGCTTT GAAAGCCACT
    CTGTCATTGC TGGAAAGGGT TTTAATGAAA GACATAGTAA CTCCCGTCCC TCAAGAAGAA
    GTGAAAAACG TTATCCGTAA ATGCTTAGAG CAAGCAGCCT TAGTTAACTA CACGAGATTA
    TCCGAATATG CCAAAATCGA AGAGAATCAA AAGGACGCAG AAAATGTAGG CCGATTAGTA
    ACGCCTGCCA AAAAGCTCGA AGATACAATA CGGCTCGCAG AACTTGTCAT TGAAGTTCTA
    CAGCAGAATG AGGAGCACCA TGCGGAGGGG AAAGAGGCGT TTGCTTGGTG GTCGGACTTG
    ATGGTGGAAC ACGCAGAGAC CTTCCTTTCG CTTTTCGCGG TGGATATGGA TGCTGCTTTA
    GAGGTGCAAC CGCCAGACAG CTGGGACAGC TTCCCTCTTT TTCAGCTAAT AAATGACTTC
    CTTCGAAGTG ACTATAATTT ATGCAATGGG AAATTCCACA AACACCTTCA AGATCTCTTT
    GCTCCACTCG TTGTGAGATA CGTCGACCTC ATGGAGTCCT CAATTGCACA GTCAATTCAC
    AGGGGCTTTG AGCGTGAATC ATGGGAACCA GTAAAGAGTT TAACAAGTAA TCTACCCAAT
    GTGAATCTAC CAAATGTGAA TCTCCCTAAA GTACCAGTTA CCTTACCAGT TAACCTCCCA
    CAAATGCCTA GTTTTTCTGC ACCGTCATGG ATGGCAGCTA TATATGATTC GGACAATGGA
    TCAGCAACTT CGGAAGATCT ATTCTGGAAG CTTGACGCTC TGCAAACGTT CATTCGAGAT
    CTTCATTGGC CCGAGGAGGA ATTCGGGAAA CACCTAGAGC AACGCCTTAA ACTTATGGCT
    AGCGATATGA TCGAATCGTG TGTCAAACGA ACTAGAATTG CATTCGAAGT CAAACTGCAG
    AAAACCAGTC GATCAACAGA CTTCCGAGTA CCGCAATCAA TATGCACCAT GTTTAATGTT
    ATGGTTGATG CCAAAGCTCA GTCAACGAAA CTTTGTAGCA TGGAAATGGG CCAAGAGCAC
    CAGTACCACT CCCAAATTGA CGAGCTAATT GAAGAAACAG TTAAAGAGAT GATCACACTC
    TTAGTGGCAA AGTTTGTCAC AATTCTAGAA GGAGTTCTGT CCAAACTATC CAGATACGAT
    GAAGGGACAT TATTTTCCTC CTTTCTGTCA TTTACTGTGA AAGCAGCTTC CAAGTATGTG
    GATGTACCAA AACCCGGGAT GGACCTTGCC GACGCCTACG TGACATTTGT GCGCCACTCT
    CAGGATGTCC TGCGTGATAA GGTCAATGAG GAGATATATA TAGAAAGGTT ATTTGATCAA
    TGGTATACCA GCTCAATGAA TGTTGTTTGT ACTTGGCTGA CCGACCGTAT GGATCTGCAA
    CTTCACATTT ATCAGCTGAA AACCCTAATT AGGGTCGTCA AGAAAACCTA CCGCGATTTC
    CGATTGCAAG GCGTCTTGGA CTCCACCTTG AACAGCAAGA CCTACGACAC CATTAGAAAC
    CGCCTCACCG TGGAAGAAGC CACAGCGTCC GTGAGCGAGG GGGGAGGCCT CCAGGGAATC
    ACCATGAAGG ACAGCGACGA GGAGGATGAG GAGGATGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815894.1
    CDS595..4536
    Translation

    Target ORF information:

    RefSeq Version XM_012960440.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012960440.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTATG CTGATAGGGC ACAAAAACAT GGCATGGATG AATTTATCTC GTCCAATCCC
    TGTAACTTTG ACCACGCCAC CCTCTTTGAG ATGGTTCAGC GACTCACACT GGATCACAGA
    CTCAACGACT CGTACTCATG TCTGGGTTGG TTCAGTCCAG GTCAGGTCTT TGTACTCGAT
    GAATATTGTG CAAGATATGG AGTCCGAGGC TGCCATAGGC ATTTGTGTTA CCTTGGTGAT
    TTACTAGAAC GAGCAGAGAA TGGGTCAATG GTCGACCCAA CTTTACTTCA CTATAGTTTT
    GCCTTCTGCG CATCCCACGT ACATGGCAAC AGGCCTGATG GCATCGGAAC TGTAACCGTG
    GAAGAAAAAG AGCGCTTTGA AGAGATCAAA GAACGTCTCC GCTTGCTTTT GGAGAATCAG
    ATCACGCATT TTAGGTACTG CTTTCCATTT GGCCGACCAG AAGGTGCTTT GAAAGCCACT
    CTGTCATTGC TGGAAAGGGT TTTAATGAAA GACATAGTAA CTCCCGTCCC TCAAGAAGAA
    GTGAAAAACG TTATCCGTAA ATGCTTAGAG CAAGCAGCCT TAGTTAACTA CACGAGATTA
    TCCGAATATG CCAAAATCGA AGAGAATCAA AAGGACGCAG AAAATGTAGG CCGATTAGTA
    ACGCCTGCCA AAAAGCTCGA AGATACAATA CGGCTCGCAG AACTTGTCAT TGAAGTTCTA
    CAGCAGAATG AGGAGCACCA TGCGGAGGGG AAAGAGGCGT TTGCTTGGTG GTCGGACTTG
    ATGGTGGAAC ACGCAGAGAC CTTCCTTTCG CTTTTCGCGG TGGATATGGA TGCTGCTTTA
    GAGGTGCAAC CGCCAGACAG CTGGGACAGC TTCCCTCTTT TTCAGCTAAT AAATGACTTC
    CTTCGAAGTG ACTATAATTT ATGCAATGGG AAATTCCACA AACACCTTCA AGATCTCTTT
    GCTCCACTCG TTGTGAGATA CGTCGACCTC ATGGAGTCCT CAATTGCACA GTCAATTCAC
    AGGGGCTTTG AGCGTGAATC ATGGGAACCA GTAAAGAGTT TAACAAGTAA TCTACCCAAT
    GTGAATCTAC CAAATGTGAA TCTCCCTAAA GTACCAGTTA CCTTACCAGT TAACCTCCCA
    CAAATGCCTA GTTTTTCTGC ACCGTCATGG ATGGCAGCTA TATATGATTC GGACAATGGA
    TCAGCAACTT CGGAAGATCT ATTCTGGAAG CTTGACGCTC TGCAAACGTT CATTCGAGAT
    CTTCATTGGC CCGAGGAGGA ATTCGGGAAA CACCTAGAGC AACGCCTTAA ACTTATGGCT
    AGCGATATGA TCGAATCGTG TGTCAAACGA ACTAGAATTG CATTCGAAGT CAAACTGCAG
    AAAACCAGTC GATCAACAGA CTTCCGAGTA CCGCAATCAA TATGCACCAT GTTTAATGTT
    ATGGTTGATG CCAAAGCTCA GTCAACGAAA CTTTGTAGCA TGGAAATGGG CCAAGAGCAC
    CAGTACCACT CCCAAATTGA CGAGCTAATT GAAGAAACAG TTAAAGAGAT GATCACACTC
    TTAGTGGCAA AGTTTGTCAC AATTCTAGAA GGAGTTCTGT CCAAACTATC CAGATACGAT
    GAAGGGACAT TATTTTCCTC CTTTCTGTCA TTTACTGTGA AAGCAGCTTC CAAGTATGTG
    GATGTACCAA AACCCGGGAT GGACCTTGCC GACGCCTACG TGACATTTGT GCGCCACTCT
    CAGGATGTCC TGCGTGATAA GGTCAATGAG GAGATATATA TAGAAAGGTT ATTTGATCAA
    TGGTATACCA GCTCAATGAA TGTTGTTTGT ACTTGGCTGA CCGACCGTAT GGATCTGCAA
    CTTCACATTT ATCAGCTGAA AACCCTAATT AGGGTCGTCA AGAAAACCTA CCGCGATTTC
    CGATTGCAAG GCGTCTTGGA CTCCACCTTG AACAGCAAGA CCTACGACAC CATTAGAAAC
    CGCCTCACCG TGGAAGAAGC CACAGCGTCC GTGAGCGAGG GGGGAGGCCT CCAGGGAATC
    ACCATGAAGG ACAGCGACGA GGAGGATGAG GAGGATGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55650
    Clone ID Related Accession (Same CDS sequence) XM_018092887.2
    Accession Version XM_018092887.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3933bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018092887.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGGGAAAA AGAGAGAAAT GTATGAACAT
    CCTGTCTTCT GCTTGGCCTC CCAAGTGATG GATTTAACCA TTCAGAATCA AAAGGACGCA
    GAAAATGTAG GCCGATTAGT AACGCCTGCC AAAAAGCTCG AAGATACAAT ACGGCTCGCA
    GAACTTGTCA TTGAAGTTCT ACAGCAGAAT GAGGAGCACC ATGCGGAGGG GAAAGAGGCG
    TTTGCTTGGT GGTCGGACTT GATGGTGGAA CACGCAGAGA CCTTCCTTTC GCTTTTCGCG
    GTGGATATGG ATGCTGCTTT AGAGGTGCAA CCGCCAGACA GCTGGGACAG CTTCCCTCTT
    TTTCAGCTAA TAAATGACTT CCTTCGAAGT GACTATAATT TATGCAATGG GAAATTCCAC
    AAACACCTTC AAGATCTCTT TGCTCCACTC GTTGTGAGAT ACGTCGACCT CATGGAGTCC
    TCAATTGCAC AGTCAATTCA CAGGGGCTTT GAGCGTGAAT CATGGGAACC AGTAAACAAT
    GGATCAGCAA CTTCGGAAGA TCTATTCTGG AAGCTTGACG CTCTGCAAAC GTTCATTCGA
    GATCTTCATT GGCCCGAGGA GGAATTCGGG AAACACCTAG AGCAACGCCT TAAACTTATG
    GCTAGCGATA TGATCGAATC GTGTGTCAAA CGAACTAGAA TTGCATTCGA AGTCAAACTG
    CAGAAAACCA GTCGATCAAC AGACTTCCGA GTACCGCAAT CAATATGCAC CATGTTTAAT
    GTTATGGTTG ATGCCAAAGC TCAGTCAACG AAACTTTGTA GCATGGAAAT GGGCCAAGAG
    CACCAGTACC ACTCCCAAAT TGACGAGCTA ATTGAAGAAA CAGTTAAAGA GATGATCACA
    CTCTTAGTGG CAAAGTTTGT CACAATTCTA GAAGGAGTTC TGTCCAAACT ATCCAGATAC
    GATGAAGGGA CATTATTTTC CTCCTTTCTG TCATTTACTG TGAAAGCAGC TTCCAAGTAT
    GTGGATGTAC CAAAACCCGG GATGGACCTT GCCGACGCCT ACGTGACATT TGTGCGCCAC
    TCTCAGGATG TCCTGCGTGA TAAGGTCAAT GAGGAGATAT ATATAGAAAG GTTATTTGAT
    AAAAGGCTGG ATGGTAATAG TTCTGTCATG TACCTGAGAA TATTGGAGCA ATGGTATACC
    AGCTCAATGA ATGTTGTTTG TACTTGGCTG ACCGACCGTA TGGATCTGCA ACTTCACATT
    TATCAGCTGA AAACCCTAAT TAGGGTCGTC AAGAAAACCT ACCGCGATTT CCGATTGCAA
    GGCGTCTTGG ACTCCACCTT GAACAGCAAG ACCTACGACA CCATTAGAAA CCGCCTCACC
    GTGGAAGAAG CCACAGCGTC CGTGAGCGAG GGGGGAGGCC TCCAGGGAAT CACCATGAAG
    GACAGCGACG AGGAGGATGA GGAGGATGAT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017948376.1
    CDS600..4532
    Translation

    Target ORF information:

    RefSeq Version XM_018092887.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092887.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGGGAAAA AGAGAGAAAT GTATGAACAT
    CCTGTCTTCT GCTTGGCCTC CCAAGTGATG GATTTAACCA TTCAGAATCA AAAGGACGCA
    GAAAATGTAG GCCGATTAGT AACGCCTGCC AAAAAGCTCG AAGATACAAT ACGGCTCGCA
    GAACTTGTCA TTGAAGTTCT ACAGCAGAAT GAGGAGCACC ATGCGGAGGG GAAAGAGGCG
    TTTGCTTGGT GGTCGGACTT GATGGTGGAA CACGCAGAGA CCTTCCTTTC GCTTTTCGCG
    GTGGATATGG ATGCTGCTTT AGAGGTGCAA CCGCCAGACA GCTGGGACAG CTTCCCTCTT
    TTTCAGCTAA TAAATGACTT CCTTCGAAGT GACTATAATT TATGCAATGG GAAATTCCAC
    AAACACCTTC AAGATCTCTT TGCTCCACTC GTTGTGAGAT ACGTCGACCT CATGGAGTCC
    TCAATTGCAC AGTCAATTCA CAGGGGCTTT GAGCGTGAAT CATGGGAACC AGTAAACAAT
    GGATCAGCAA CTTCGGAAGA TCTATTCTGG AAGCTTGACG CTCTGCAAAC GTTCATTCGA
    GATCTTCATT GGCCCGAGGA GGAATTCGGG AAACACCTAG AGCAACGCCT TAAACTTATG
    GCTAGCGATA TGATCGAATC GTGTGTCAAA CGAACTAGAA TTGCATTCGA AGTCAAACTG
    CAGAAAACCA GTCGATCAAC AGACTTCCGA GTACCGCAAT CAATATGCAC CATGTTTAAT
    GTTATGGTTG ATGCCAAAGC TCAGTCAACG AAACTTTGTA GCATGGAAAT GGGCCAAGAG
    CACCAGTACC ACTCCCAAAT TGACGAGCTA ATTGAAGAAA CAGTTAAAGA GATGATCACA
    CTCTTAGTGG CAAAGTTTGT CACAATTCTA GAAGGAGTTC TGTCCAAACT ATCCAGATAC
    GATGAAGGGA CATTATTTTC CTCCTTTCTG TCATTTACTG TGAAAGCAGC TTCCAAGTAT
    GTGGATGTAC CAAAACCCGG GATGGACCTT GCCGACGCCT ACGTGACATT TGTGCGCCAC
    TCTCAGGATG TCCTGCGTGA TAAGGTCAAT GAGGAGATAT ATATAGAAAG GTTATTTGAT
    AAAAGGCTGG ATGGTAATAG TTCTGTCATG TACCTGAGAA TATTGGAGCA ATGGTATACC
    AGCTCAATGA ATGTTGTTTG TACTTGGCTG ACCGACCGTA TGGATCTGCA ACTTCACATT
    TATCAGCTGA AAACCCTAAT TAGGGTCGTC AAGAAAACCT ACCGCGATTT CCGATTGCAA
    GGCGTCTTGG ACTCCACCTT GAACAGCAAG ACCTACGACA CCATTAGAAA CCGCCTCACC
    GTGGAAGAAG CCACAGCGTC CGTGAGCGAG GGGGGAGGCC TCCAGGGAAT CACCATGAAG
    GACAGCGACG AGGAGGATGA GGAGGATGAT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55651
    Clone ID Related Accession (Same CDS sequence) XM_012960433.3
    Accession Version XM_012960433.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3906bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012960433.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGGGAAAA AGAGAGAAAT GTATGAACAT
    CCTGTCTTCT GCTTGGCCTC CCAAGTGATG GATTTAACCA TTCAAAATGT AGGCCGATTA
    GTAACGCCTG CCAAAAAGCT CGAAGATACA ATACGGCTCG CAGAACTTGT CATTGAAGTT
    CTACAGCAGA ATGAGGAGCA CCATGCGGAG GCGTTTGCTT GGTGGTCGGA CTTGATGGTG
    GAACACGCAG AGACCTTCCT TTCGCTTTTC GCGGTGGATA TGGATGCTGC TTTAGAGGTG
    CAACCGCCAG ACAGCTGGGA CAGCTTCCCT CTTTTTCAGC TAATAAATGA CTTCCTTCGA
    AGTGACTATA ATTTATGCAA TGGGAAATTC CACAAACACC TTCAAGATCT CTTTGCTCCA
    CTCGTTGTGA GATACGTCGA CCTCATGGAG TCCTCAATTG CACAGTCAAT TCACAGGGGC
    TTTGAGCGTG AATCATGGGA ACCAGTAAAC AATGGATCAG CAACTTCGGA AGATCTATTC
    TGGAAGCTTG ACGCTCTGCA AACGTTCATT CGAGATCTTC ATTGGCCCGA GGAGGAATTC
    GGGAAACACC TAGAGCAACG CCTTAAACTT ATGGCTAGCG ATATGATCGA ATCGTGTGTC
    AAACGAACTA GAATTGCATT CGAAGTCAAA CTGCAGAAAA CCAGTCGATC AACAGACTTC
    CGAGTACCGC AATCAATATG CACCATGTTT AATGTTATGG TTGATGCCAA AGCTCAGTCA
    ACGAAACTTT GTAGCATGGA AATGGGCCAA GAGCACCAGT ACCACTCCCA AATTGACGAG
    CTAATTGAAG AAACAGTTAA AGAGATGATC ACACTCTTAG TGGCAAAGTT TGTCACAATT
    CTAGAAGGAG TTCTGTCCAA ACTATCCAGA TACGATGAAG GGACATTATT TTCCTCCTTT
    CTGTCATTTA CTGTGAAAGC AGCTTCCAAG TATGTGGATG TACCAAAACC CGGGATGGAC
    CTTGCCGACG CCTACGTGAC ATTTGTGCGC CACTCTCAGG ATGTCCTGCG TGATAAGGTC
    AATGAGGAGA TATATATAGA AAGGTTATTT GATAAAAGGC TGGATGGTAA TAGTTCTGTC
    ATGTACCTGA GAATATTGGA GCAATGGTAT ACCAGCTCAA TGAATGTTGT TTGTACTTGG
    CTGACCGACC GTATGGATCT GCAACTTCAC ATTTATCAGC TGAAAACCCT AATTAGGGTC
    GTCAAGAAAA CCTACCGCGA TTTCCGATTG CAAGGCGTCT TGGACTCCAC CTTGAACAGC
    AAGACCTACG ACACCATTAG AAACCGCCTC ACCGTGGAAG AAGCCACAGC GTCCGTGAGC
    GAGGGGGGAG GCCTCCAGGG AATCACCATG AAGGACAGCG ACGAGGAGGA TGAGGAGGAT
    GATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815887.1
    CDS599..4504
    Translation

    Target ORF information:

    RefSeq Version XM_012960433.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012960433.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGGGAAAA AGAGAGAAAT GTATGAACAT
    CCTGTCTTCT GCTTGGCCTC CCAAGTGATG GATTTAACCA TTCAAAATGT AGGCCGATTA
    GTAACGCCTG CCAAAAAGCT CGAAGATACA ATACGGCTCG CAGAACTTGT CATTGAAGTT
    CTACAGCAGA ATGAGGAGCA CCATGCGGAG GCGTTTGCTT GGTGGTCGGA CTTGATGGTG
    GAACACGCAG AGACCTTCCT TTCGCTTTTC GCGGTGGATA TGGATGCTGC TTTAGAGGTG
    CAACCGCCAG ACAGCTGGGA CAGCTTCCCT CTTTTTCAGC TAATAAATGA CTTCCTTCGA
    AGTGACTATA ATTTATGCAA TGGGAAATTC CACAAACACC TTCAAGATCT CTTTGCTCCA
    CTCGTTGTGA GATACGTCGA CCTCATGGAG TCCTCAATTG CACAGTCAAT TCACAGGGGC
    TTTGAGCGTG AATCATGGGA ACCAGTAAAC AATGGATCAG CAACTTCGGA AGATCTATTC
    TGGAAGCTTG ACGCTCTGCA AACGTTCATT CGAGATCTTC ATTGGCCCGA GGAGGAATTC
    GGGAAACACC TAGAGCAACG CCTTAAACTT ATGGCTAGCG ATATGATCGA ATCGTGTGTC
    AAACGAACTA GAATTGCATT CGAAGTCAAA CTGCAGAAAA CCAGTCGATC AACAGACTTC
    CGAGTACCGC AATCAATATG CACCATGTTT AATGTTATGG TTGATGCCAA AGCTCAGTCA
    ACGAAACTTT GTAGCATGGA AATGGGCCAA GAGCACCAGT ACCACTCCCA AATTGACGAG
    CTAATTGAAG AAACAGTTAA AGAGATGATC ACACTCTTAG TGGCAAAGTT TGTCACAATT
    CTAGAAGGAG TTCTGTCCAA ACTATCCAGA TACGATGAAG GGACATTATT TTCCTCCTTT
    CTGTCATTTA CTGTGAAAGC AGCTTCCAAG TATGTGGATG TACCAAAACC CGGGATGGAC
    CTTGCCGACG CCTACGTGAC ATTTGTGCGC CACTCTCAGG ATGTCCTGCG TGATAAGGTC
    AATGAGGAGA TATATATAGA AAGGTTATTT GATAAAAGGC TGGATGGTAA TAGTTCTGTC
    ATGTACCTGA GAATATTGGA GCAATGGTAT ACCAGCTCAA TGAATGTTGT TTGTACTTGG
    CTGACCGACC GTATGGATCT GCAACTTCAC ATTTATCAGC TGAAAACCCT AATTAGGGTC
    GTCAAGAAAA CCTACCGCGA TTTCCGATTG CAAGGCGTCT TGGACTCCAC CTTGAACAGC
    AAGACCTACG ACACCATTAG AAACCGCCTC ACCGTGGAAG AAGCCACAGC GTCCGTGAGC
    GAGGGGGGAG GCCTCCAGGG AATCACCATG AAGGACAGCG ACGAGGAGGA TGAGGAGGAT
    GATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55652
    Clone ID Related Accession (Same CDS sequence) XM_012960434.3
    Accession Version XM_012960434.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3900bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012960434.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTATG CTGATAGGGC ACAAAAACAT GGCATGGATG AATTTATCTC GTCCAATCCC
    TGTAACTTTG ACCACGCCAC CCTCTTTGAG ATGGTTCAGC GACTCACACT GGATCACAGA
    CTCAACGACT CGTACTCATG TCTGGGTTGG TTCAGTCCAG GTCAGGTCTT TGTACTCGAT
    GAATATTGTG CAAGATATGG AGTCCGAGGC TGCCATAGGC ATTTGTGTTA CCTTGGTGAT
    TTACTAGAAC GAGCAGAGAA TGGGTCAATG GTCGACCCAA CTTTACTTCA CTATAGTTTT
    GCCTTCTGCG CATCCCACGT ACATGGCAAC AGGCCTGATG GCATCGGAAC TGTAACCGTG
    GAAGAAAAAG AGCGCTTTGA AGAGATCAAA GAACGTCTCC GCTTGCTTTT GGAGAATCAG
    ATCACGCATT TTAGGTACTG CTTTCCATTT GGCCGACCAG AAGGTGCTTT GAAAGCCACT
    CTGTCATTGC TGGAAAGGGT TTTAATGAAA GACATAGTAA CTCCCGTCCC TCAAGAAGAA
    GTGAAAAACG TTATCCGTAA ATGCTTAGAG CAAGCAGCCT TAGTTAACTA CACGAGATTA
    TCCGAATATG CCAAAATCGA AGAGAATCAA AAGGACGCAG AAAATGTAGG CCGATTAGTA
    ACGCCTGCCA AAAAGCTCGA AGATACAATA CGGCTCGCAG AACTTGTCAT TGAAGTTCTA
    CAGCAGAATG AGGAGCACCA TGCGGAGGCG TTTGCTTGGT GGTCGGACTT GATGGTGGAA
    CACGCAGAGA CCTTCCTTTC GCTTTTCGCG GTGGATATGG ATGCTGCTTT AGAGGTGCAA
    CCGCCAGACA GCTGGGACAG CTTCCCTCTT TTTCAGCTAA TAAATGACTT CCTTCGAAGT
    GACTATAATT TATGCAATGG GAAATTCCAC AAACACCTTC AAGATCTCTT TGCTCCACTC
    GTTGTGAGAT ACGTCGACCT CATGGAGTCC TCAATTGCAC AGTCAATTCA CAGGGGCTTT
    GAGCGTGAAT CATGGGAACC AGTAAAGAGT TTAACAAGTA ATCTACCCAA TGTGAATCTA
    CCAAATGTGA ATCTCCCTAA AGTACCAGTT ACCTTACCAG TTAACCTCCC ACAAATGCCT
    AGTTTTTCTG CACCGTCATG GATGGCAGCT ATATATGATT CGGACAATGG ATCAGCAACT
    TCGGAAGATC TATTCTGGAA GCTTGACGCT CTGCAAACGT TCATTCGAGA TCTTCATTGG
    CCCGAGGAGG AATTCGGGAA ACACCTAGAG CAACGCCTTA AACTTATGGC TAGCGATATG
    ATCGAATCGT GTGTCAAACG AACTAGAATT GCATTCGAAG TCAAACTGCA GAAAACCAGT
    CGATCAACAG ACTTCCGAGT ACCGCAATCA ATATGCACCA TGTTTAATGT TATGGTTGAT
    GCCAAAGCTC AGTCAACGAA ACTTTGTAGC ATGGAAATGG GCCAAGAGCA CCAGTACCAC
    TCCCAAATTG ACGAGCTAAT TGAAGAAACA GTTAAAGAGA TGATCACACT CTTAGTGGCA
    AAGTTTGTCA CAATTCTAGA AGGAGTTCTG TCCAAACTAT CCAGATACGA TGAAGGGACA
    TTATTTTCCT CCTTTCTGTC ATTTACTAAA CCCGGGATGG ACCTTGCCGA CGCCTACGTG
    ACATTTGTGC GCCACTCTCA GGATGTCCTG CGTGATAAGG TCAATGAGGA GATATATATA
    GAAAGGTTAT TTGATCAATG GTATACCAGC TCAATGAATG TTGTTTGTAC TTGGCTGACC
    GACCGTATGG ATCTGCAACT TCACATTTAT CAGCTGAAAA CCCTAATTAG GGTCGTCAAG
    AAAACCTACC GCGATTTCCG ATTGCAAGGC GTCTTGGACT CCACCTTGAA CAGCAAGACC
    TACGACACCA TTAGAAACCG CCTCACCGTG GAAGAAGCCA CAGCGTCCGT GAGCGAGGGG
    GGAGGCCTCC AGGGAATCAC CATGAAGGAC AGCGACGAGG AGGATGAGGA GGATGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815888.1
    CDS595..4494
    Translation

    Target ORF information:

    RefSeq Version XM_012960434.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012960434.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTATG CTGATAGGGC ACAAAAACAT GGCATGGATG AATTTATCTC GTCCAATCCC
    TGTAACTTTG ACCACGCCAC CCTCTTTGAG ATGGTTCAGC GACTCACACT GGATCACAGA
    CTCAACGACT CGTACTCATG TCTGGGTTGG TTCAGTCCAG GTCAGGTCTT TGTACTCGAT
    GAATATTGTG CAAGATATGG AGTCCGAGGC TGCCATAGGC ATTTGTGTTA CCTTGGTGAT
    TTACTAGAAC GAGCAGAGAA TGGGTCAATG GTCGACCCAA CTTTACTTCA CTATAGTTTT
    GCCTTCTGCG CATCCCACGT ACATGGCAAC AGGCCTGATG GCATCGGAAC TGTAACCGTG
    GAAGAAAAAG AGCGCTTTGA AGAGATCAAA GAACGTCTCC GCTTGCTTTT GGAGAATCAG
    ATCACGCATT TTAGGTACTG CTTTCCATTT GGCCGACCAG AAGGTGCTTT GAAAGCCACT
    CTGTCATTGC TGGAAAGGGT TTTAATGAAA GACATAGTAA CTCCCGTCCC TCAAGAAGAA
    GTGAAAAACG TTATCCGTAA ATGCTTAGAG CAAGCAGCCT TAGTTAACTA CACGAGATTA
    TCCGAATATG CCAAAATCGA AGAGAATCAA AAGGACGCAG AAAATGTAGG CCGATTAGTA
    ACGCCTGCCA AAAAGCTCGA AGATACAATA CGGCTCGCAG AACTTGTCAT TGAAGTTCTA
    CAGCAGAATG AGGAGCACCA TGCGGAGGCG TTTGCTTGGT GGTCGGACTT GATGGTGGAA
    CACGCAGAGA CCTTCCTTTC GCTTTTCGCG GTGGATATGG ATGCTGCTTT AGAGGTGCAA
    CCGCCAGACA GCTGGGACAG CTTCCCTCTT TTTCAGCTAA TAAATGACTT CCTTCGAAGT
    GACTATAATT TATGCAATGG GAAATTCCAC AAACACCTTC AAGATCTCTT TGCTCCACTC
    GTTGTGAGAT ACGTCGACCT CATGGAGTCC TCAATTGCAC AGTCAATTCA CAGGGGCTTT
    GAGCGTGAAT CATGGGAACC AGTAAAGAGT TTAACAAGTA ATCTACCCAA TGTGAATCTA
    CCAAATGTGA ATCTCCCTAA AGTACCAGTT ACCTTACCAG TTAACCTCCC ACAAATGCCT
    AGTTTTTCTG CACCGTCATG GATGGCAGCT ATATATGATT CGGACAATGG ATCAGCAACT
    TCGGAAGATC TATTCTGGAA GCTTGACGCT CTGCAAACGT TCATTCGAGA TCTTCATTGG
    CCCGAGGAGG AATTCGGGAA ACACCTAGAG CAACGCCTTA AACTTATGGC TAGCGATATG
    ATCGAATCGT GTGTCAAACG AACTAGAATT GCATTCGAAG TCAAACTGCA GAAAACCAGT
    CGATCAACAG ACTTCCGAGT ACCGCAATCA ATATGCACCA TGTTTAATGT TATGGTTGAT
    GCCAAAGCTC AGTCAACGAA ACTTTGTAGC ATGGAAATGG GCCAAGAGCA CCAGTACCAC
    TCCCAAATTG ACGAGCTAAT TGAAGAAACA GTTAAAGAGA TGATCACACT CTTAGTGGCA
    AAGTTTGTCA CAATTCTAGA AGGAGTTCTG TCCAAACTAT CCAGATACGA TGAAGGGACA
    TTATTTTCCT CCTTTCTGTC ATTTACTAAA CCCGGGATGG ACCTTGCCGA CGCCTACGTG
    ACATTTGTGC GCCACTCTCA GGATGTCCTG CGTGATAAGG TCAATGAGGA GATATATATA
    GAAAGGTTAT TTGATCAATG GTATACCAGC TCAATGAATG TTGTTTGTAC TTGGCTGACC
    GACCGTATGG ATCTGCAACT TCACATTTAT CAGCTGAAAA CCCTAATTAG GGTCGTCAAG
    AAAACCTACC GCGATTTCCG ATTGCAAGGC GTCTTGGACT CCACCTTGAA CAGCAAGACC
    TACGACACCA TTAGAAACCG CCTCACCGTG GAAGAAGCCA CAGCGTCCGT GAGCGAGGGG
    GGAGGCCTCC AGGGAATCAC CATGAAGGAC AGCGACGAGG AGGATGAGGA GGATGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55653
    Clone ID Related Accession (Same CDS sequence) XM_031899728.1
    Accession Version XM_031899728.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3885bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGGGAAAA AGAGAGAAAT GTATGAACAT
    CCTGTCTTCT GCTTGGCCTC CCAAGTGATG GATTTAACCA TTCAGAATCA AAAGGACGCA
    GAAAATGTAG GCCGATTAGT AACGCCTGCC AAAAAGCTCG AAGATACAAT ACGGCTCGCA
    GAACTTGTCA TTGAAGTTCT ACAGCAGAAT GAGGAGCACC ATGCGGAGGG GAAAGAGGCG
    TTTGCTTGGT GGTCGGACTT GATGGTGGAA CACGCAGAGA CCTTCCTTTC GCTTTTCGCG
    GTGGATATGG ATGCTGCTTT AGAGGTGCAA CCGCCAGACA GCTGGGACAG CTTCCCTCTT
    TTTCAGCTAA TAAATGACTT CCTTCGAAGT GACTATAATT TATGCAATGG GAAATTCCAC
    AAACACCTTC AAGATCTCTT TGCTCCACTC GTTGTGAGAT ACGTCGACCT CATGGAGTCC
    TCAATTGCAC AGTCAATTCA CAGGGGCTTT GAGCGTGAAT CATGGGAACC AGTAAACAAT
    GGATCAGCAA CTTCGGAAGA TCTATTCTGG AAGCTTGACG CTCTGCAAAC GTTCATTCGA
    GATCTTCATT GGCCCGAGGA GGAATTCGGG AAACACCTAG AGCAACGCCT TAAACTTATG
    GCTAGCGATA TGATCGAATC GTGTGTCAAA CGAACTAGAA TTGCATTCGA AGTCAAACTG
    CAGAAAACCA GTCGATCAAC AGACTTCCGA GTACCGCAAT CAATATGCAC CATGTTTAAT
    GTTATGGTTG ATGCCAAAGC TCAGTCAACG AAACTTTGTA GCATGGAAAT GGGCCAAGAG
    CACCAGTACC ACTCCCAAAT TGACGAGCTA ATTGAAGAAA CAGTTAAAGA GATGATCACA
    CTCTTAGTGG CAAAGTTTGT CACAATTCTA GAAGGAGTTC TGTCCAAACT ATCCAGATAC
    GATGAAGGGA CATTATTTTC CTCCTTTCTG TCATTTACTG TGAAAGCAGC TTCCAAGTAT
    GTGGATGTAC CAAAACCCGG GATGGACCTT GCCGACGCCT ACGTGACATT TGTGCGCCAC
    TCTCAGGATG TCCTGCGTGA TAAGGTCAAT GAGGAGATAT ATATAGAAAG GTTATTTGAT
    CAATGGTATA CCAGCTCAAT GAATGTTGTT TGTACTTGGC TGACCGACCG TATGGATCTG
    CAACTTCACA TTTATCAGCT GAAAACCCTA ATTAGGGTCG TCAAGAAAAC CTACCGCGAT
    TTCCGATTGC AAGGCGTCTT GGACTCCACC TTGAACAGCA AGACCTACGA CACCATTAGA
    AACCGCCTCA CCGTGGAAGA AGCCACAGCG TCCGTGAGCG AGGGGGGAGG CCTCCAGGGA
    ATCACCATGA AGGACAGCGA CGAGGAGGAT GAGGAGGATG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031755588.1
    CDS598..4482
    Translation

    Target ORF information:

    RefSeq Version XM_031899728.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899728.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGGGAAAA AGAGAGAAAT GTATGAACAT
    CCTGTCTTCT GCTTGGCCTC CCAAGTGATG GATTTAACCA TTCAGAATCA AAAGGACGCA
    GAAAATGTAG GCCGATTAGT AACGCCTGCC AAAAAGCTCG AAGATACAAT ACGGCTCGCA
    GAACTTGTCA TTGAAGTTCT ACAGCAGAAT GAGGAGCACC ATGCGGAGGG GAAAGAGGCG
    TTTGCTTGGT GGTCGGACTT GATGGTGGAA CACGCAGAGA CCTTCCTTTC GCTTTTCGCG
    GTGGATATGG ATGCTGCTTT AGAGGTGCAA CCGCCAGACA GCTGGGACAG CTTCCCTCTT
    TTTCAGCTAA TAAATGACTT CCTTCGAAGT GACTATAATT TATGCAATGG GAAATTCCAC
    AAACACCTTC AAGATCTCTT TGCTCCACTC GTTGTGAGAT ACGTCGACCT CATGGAGTCC
    TCAATTGCAC AGTCAATTCA CAGGGGCTTT GAGCGTGAAT CATGGGAACC AGTAAACAAT
    GGATCAGCAA CTTCGGAAGA TCTATTCTGG AAGCTTGACG CTCTGCAAAC GTTCATTCGA
    GATCTTCATT GGCCCGAGGA GGAATTCGGG AAACACCTAG AGCAACGCCT TAAACTTATG
    GCTAGCGATA TGATCGAATC GTGTGTCAAA CGAACTAGAA TTGCATTCGA AGTCAAACTG
    CAGAAAACCA GTCGATCAAC AGACTTCCGA GTACCGCAAT CAATATGCAC CATGTTTAAT
    GTTATGGTTG ATGCCAAAGC TCAGTCAACG AAACTTTGTA GCATGGAAAT GGGCCAAGAG
    CACCAGTACC ACTCCCAAAT TGACGAGCTA ATTGAAGAAA CAGTTAAAGA GATGATCACA
    CTCTTAGTGG CAAAGTTTGT CACAATTCTA GAAGGAGTTC TGTCCAAACT ATCCAGATAC
    GATGAAGGGA CATTATTTTC CTCCTTTCTG TCATTTACTG TGAAAGCAGC TTCCAAGTAT
    GTGGATGTAC CAAAACCCGG GATGGACCTT GCCGACGCCT ACGTGACATT TGTGCGCCAC
    TCTCAGGATG TCCTGCGTGA TAAGGTCAAT GAGGAGATAT ATATAGAAAG GTTATTTGAT
    CAATGGTATA CCAGCTCAAT GAATGTTGTT TGTACTTGGC TGACCGACCG TATGGATCTG
    CAACTTCACA TTTATCAGCT GAAAACCCTA ATTAGGGTCG TCAAGAAAAC CTACCGCGAT
    TTCCGATTGC AAGGCGTCTT GGACTCCACC TTGAACAGCA AGACCTACGA CACCATTAGA
    AACCGCCTCA CCGTGGAAGA AGCCACAGCG TCCGTGAGCG AGGGGGGAGG CCTCCAGGGA
    ATCACCATGA AGGACAGCGA CGAGGAGGAT GAGGAGGATG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55654
    Clone ID Related Accession (Same CDS sequence) XM_031899729.1
    Accession Version XM_031899729.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3876bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGGGAAAA AGAGAGAAAT GTATGAACAT
    CCTGTCTTCT GCTTGGCCTC CCAAGTGATG GATTTAACCA TTCAGAATCA AAAGGACGCA
    GAAAATGTAG GCCGATTAGT AACGCCTGCC AAAAAGCTCG AAGATACAAT ACGGCTCGCA
    GAACTTGTCA TTGAAGTTCT ACAGCAGAAT GAGGAGCACC ATGCGGAGGC GTTTGCTTGG
    TGGTCGGACT TGATGGTGGA ACACGCAGAG ACCTTCCTTT CGCTTTTCGC GGTGGATATG
    GATGCTGCTT TAGAGGTGCA ACCGCCAGAC AGCTGGGACA GCTTCCCTCT TTTTCAGCTA
    ATAAATGACT TCCTTCGAAG TGACTATAAT TTATGCAATG GGAAATTCCA CAAACACCTT
    CAAGATCTCT TTGCTCCACT CGTTGTGAGA TACGTCGACC TCATGGAGTC CTCAATTGCA
    CAGTCAATTC ACAGGGGCTT TGAGCGTGAA TCATGGGAAC CAGTAAACAA TGGATCAGCA
    ACTTCGGAAG ATCTATTCTG GAAGCTTGAC GCTCTGCAAA CGTTCATTCG AGATCTTCAT
    TGGCCCGAGG AGGAATTCGG GAAACACCTA GAGCAACGCC TTAAACTTAT GGCTAGCGAT
    ATGATCGAAT CGTGTGTCAA ACGAACTAGA ATTGCATTCG AAGTCAAACT GCAGAAAACC
    AGTCGATCAA CAGACTTCCG AGTACCGCAA TCAATATGCA CCATGTTTAA TGTTATGGTT
    GATGCCAAAG CTCAGTCAAC GAAACTTTGT AGCATGGAAA TGGGCCAAGA GCACCAGTAC
    CACTCCCAAA TTGACGAGCT AATTGAAGAA ACAGTTAAAG AGATGATCAC ACTCTTAGTG
    GCAAAGTTTG TCACAATTCT AGAAGGAGTT CTGTCCAAAC TATCCAGATA CGATGAAGGG
    ACATTATTTT CCTCCTTTCT GTCATTTACT GTGAAAGCAG CTTCCAAGTA TGTGGATGTA
    CCAAAACCCG GGATGGACCT TGCCGACGCC TACGTGACAT TTGTGCGCCA CTCTCAGGAT
    GTCCTGCGTG ATAAGGTCAA TGAGGAGATA TATATAGAAA GGTTATTTGA TCAATGGTAT
    ACCAGCTCAA TGAATGTTGT TTGTACTTGG CTGACCGACC GTATGGATCT GCAACTTCAC
    ATTTATCAGC TGAAAACCCT AATTAGGGTC GTCAAGAAAA CCTACCGCGA TTTCCGATTG
    CAAGGCGTCT TGGACTCCAC CTTGAACAGC AAGACCTACG ACACCATTAG AAACCGCCTC
    ACCGTGGAAG AAGCCACAGC GTCCGTGAGC GAGGGGGGAG GCCTCCAGGG AATCACCATG
    AAGGACAGCG ACGAGGAGGA TGAGGAGGAT GATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031755589.1
    CDS597..4472
    Translation

    Target ORF information:

    RefSeq Version XM_031899729.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899729.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGGGAAAA AGAGAGAAAT GTATGAACAT
    CCTGTCTTCT GCTTGGCCTC CCAAGTGATG GATTTAACCA TTCAGAATCA AAAGGACGCA
    GAAAATGTAG GCCGATTAGT AACGCCTGCC AAAAAGCTCG AAGATACAAT ACGGCTCGCA
    GAACTTGTCA TTGAAGTTCT ACAGCAGAAT GAGGAGCACC ATGCGGAGGC GTTTGCTTGG
    TGGTCGGACT TGATGGTGGA ACACGCAGAG ACCTTCCTTT CGCTTTTCGC GGTGGATATG
    GATGCTGCTT TAGAGGTGCA ACCGCCAGAC AGCTGGGACA GCTTCCCTCT TTTTCAGCTA
    ATAAATGACT TCCTTCGAAG TGACTATAAT TTATGCAATG GGAAATTCCA CAAACACCTT
    CAAGATCTCT TTGCTCCACT CGTTGTGAGA TACGTCGACC TCATGGAGTC CTCAATTGCA
    CAGTCAATTC ACAGGGGCTT TGAGCGTGAA TCATGGGAAC CAGTAAACAA TGGATCAGCA
    ACTTCGGAAG ATCTATTCTG GAAGCTTGAC GCTCTGCAAA CGTTCATTCG AGATCTTCAT
    TGGCCCGAGG AGGAATTCGG GAAACACCTA GAGCAACGCC TTAAACTTAT GGCTAGCGAT
    ATGATCGAAT CGTGTGTCAA ACGAACTAGA ATTGCATTCG AAGTCAAACT GCAGAAAACC
    AGTCGATCAA CAGACTTCCG AGTACCGCAA TCAATATGCA CCATGTTTAA TGTTATGGTT
    GATGCCAAAG CTCAGTCAAC GAAACTTTGT AGCATGGAAA TGGGCCAAGA GCACCAGTAC
    CACTCCCAAA TTGACGAGCT AATTGAAGAA ACAGTTAAAG AGATGATCAC ACTCTTAGTG
    GCAAAGTTTG TCACAATTCT AGAAGGAGTT CTGTCCAAAC TATCCAGATA CGATGAAGGG
    ACATTATTTT CCTCCTTTCT GTCATTTACT GTGAAAGCAG CTTCCAAGTA TGTGGATGTA
    CCAAAACCCG GGATGGACCT TGCCGACGCC TACGTGACAT TTGTGCGCCA CTCTCAGGAT
    GTCCTGCGTG ATAAGGTCAA TGAGGAGATA TATATAGAAA GGTTATTTGA TCAATGGTAT
    ACCAGCTCAA TGAATGTTGT TTGTACTTGG CTGACCGACC GTATGGATCT GCAACTTCAC
    ATTTATCAGC TGAAAACCCT AATTAGGGTC GTCAAGAAAA CCTACCGCGA TTTCCGATTG
    CAAGGCGTCT TGGACTCCAC CTTGAACAGC AAGACCTACG ACACCATTAG AAACCGCCTC
    ACCGTGGAAG AAGCCACAGC GTCCGTGAGC GAGGGGGGAG GCCTCCAGGG AATCACCATG
    AAGGACAGCG ACGAGGAGGA TGAGGAGGAT GATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55655
    Clone ID Related Accession (Same CDS sequence) XM_012960435.3
    Accession Version XM_012960435.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3846bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012960435.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTATG CTGATAGGGC ACAAAAACAT GGCATGGATG AATTTATCTC GTCCAATCCC
    TGTAACTTTG ACCACGCCAC CCTCTTTGAG ATGGTTCAGC GACTCACACT GGATCACAGA
    CTCAACGACT CGTACTCATG TCTGGGTTGG TTCAGTCCAG GTCAGGTCTT TGTACTCGAT
    GAATATTGTG CAAGATATGG AGTCCGAGGC TGCCATAGGC ATTTGTGTTA CCTTGGTGAT
    TTACTAGAAC GAGCAGAGAA TGGGTCAATG GTCGACCCAA CTTTACTTCA CTATAGTTTT
    GCCTTCTGCG CATCCCACGT ACATGGCAAC AGGCCTGATG GCATCGGAAC TGTAACCGTG
    GAAGAAAAAG AGCGCTTTGA AGAGATCAAA GAACGTCTCC GCTTGCTTTT GGAGAATCAG
    ATCACGCATT TTAGGTACTG CTTTCCATTT GGCCGACCAG AAGGTGCTTT GAAAGCCACT
    CTGTCATTGC TGGAAAGGGT TTTAATGAAA GACATAGTAA CTCCCGTCCC TCAAGAAGAA
    GTGAAAAACG TTATCCGTAA ATGCTTAGAG CAAGCAGCCT TAGTTAACTA CACGAGATTA
    TCCGAATATG CCAAAATCGA AGGGAAAAAG AGAGAAATGT ATGAACATCC TGTCTTCTGC
    TTGGCCTCCC AAGTGATGGA TTTAACCATT CAAAATGTAG GCCGATTAGT AACGCCTGCC
    AAAAAGCTCG AAGATACAAT ACGGCTCGCA GAACTTGTCA TTGAAGTTCT ACAGCAGAAT
    GAGGAGCACC ATGCGGAGGC GTTTGCTTGG TGGTCGGACT TGATGGTGGA ACACGCAGAG
    ACCTTCCTTT CGCTTTTCGC GGTGGATATG GATGCTGCTT TAGAGGTGCA ACCGCCAGAC
    AGCTGGGACA GCTTCCCTCT TTTTCAGCTA ATAAATGACT TCCTTCGAAG TGACTATAAT
    TTATGCAATG GGAAATTCCA CAAACACCTT CAAGATCTCT TTGCTCCACT CGTTGTGAGA
    TACGTCGACC TCATGGAGTC CTCAATTGCA CAGTCAATTC ACAGGGGCTT TGAGCGTGAA
    TCATGGGAAC CAGTAAACAA TGGATCAGCA ACTTCGGAAG ATCTATTCTG GAAGCTTGAC
    GCTCTGCAAA CGTTCATTCG AGATCTTCAT TGGCCCGAGG AGGAATTCGG GAAACACCTA
    GAGCAACGCC TTAAACTTAT GGCTAGCGAT ATGATCGAAT CGTGTGTCAA ACGAACTAGA
    ATTGCATTCG AAGTCAAACT GCAGAAAACC AGTCGATCAA CAGACTTCCG AGTACCGCAA
    TCAATATGCA CCATGTTTAA TGTTATGGTT GATGCCAAAG CTCAGTCAAC GAAACTTTGT
    AGCATGGAAA TGGGCCAAGA GCACCAGTAC CACTCCCAAA TTGACGAGCT AATTGAAGAA
    ACAGTTAAAG AGATGATCAC ACTCTTAGTG GCAAAGTTTG TCACAATTCT AGAAGGAGTT
    CTGTCCAAAC TATCCAGATA CGATGAAGGG ACATTATTTT CCTCCTTTCT GTCATTTACT
    GTGAAAGCAG CTTCCAAGTA TGTGGATGTA CCAAAACCCG GGATGGACCT TGCCGACGCC
    TACGTGACAT TTGTGCGCCA CTCTCAGGAT GTCCTGCGTG ATAAGGTCAA TGAGGAGATA
    TATATAGAAA GGTTATTTGA TCAATGGTAT ACCAGCTCAA TGAATGTTGT TTGTACTTGG
    CTGACCGACC GTATGGATCT GCAACTTCAC ATTTATCAGC TGAAAACCCT AATTAGGGTC
    GTCAAGAAAA CCTACCGCGA TTTCCGATTG CAAGGCGTCT TGGACTCCAC CTTGAACAGC
    AAGACCTACG ACACCATTAG AAACCGCCTC ACCGTGGAAG AAGCCACAGC GTCCGTGAGC
    GAGGGGGGAG GCCTCCAGGG AATCACCATG AAGGACAGCG ACGAGGAGGA TGAGGAGGAT
    GATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815889.1
    CDS595..4440
    Translation

    Target ORF information:

    RefSeq Version XM_012960435.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012960435.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTATG CTGATAGGGC ACAAAAACAT GGCATGGATG AATTTATCTC GTCCAATCCC
    TGTAACTTTG ACCACGCCAC CCTCTTTGAG ATGGTTCAGC GACTCACACT GGATCACAGA
    CTCAACGACT CGTACTCATG TCTGGGTTGG TTCAGTCCAG GTCAGGTCTT TGTACTCGAT
    GAATATTGTG CAAGATATGG AGTCCGAGGC TGCCATAGGC ATTTGTGTTA CCTTGGTGAT
    TTACTAGAAC GAGCAGAGAA TGGGTCAATG GTCGACCCAA CTTTACTTCA CTATAGTTTT
    GCCTTCTGCG CATCCCACGT ACATGGCAAC AGGCCTGATG GCATCGGAAC TGTAACCGTG
    GAAGAAAAAG AGCGCTTTGA AGAGATCAAA GAACGTCTCC GCTTGCTTTT GGAGAATCAG
    ATCACGCATT TTAGGTACTG CTTTCCATTT GGCCGACCAG AAGGTGCTTT GAAAGCCACT
    CTGTCATTGC TGGAAAGGGT TTTAATGAAA GACATAGTAA CTCCCGTCCC TCAAGAAGAA
    GTGAAAAACG TTATCCGTAA ATGCTTAGAG CAAGCAGCCT TAGTTAACTA CACGAGATTA
    TCCGAATATG CCAAAATCGA AGGGAAAAAG AGAGAAATGT ATGAACATCC TGTCTTCTGC
    TTGGCCTCCC AAGTGATGGA TTTAACCATT CAAAATGTAG GCCGATTAGT AACGCCTGCC
    AAAAAGCTCG AAGATACAAT ACGGCTCGCA GAACTTGTCA TTGAAGTTCT ACAGCAGAAT
    GAGGAGCACC ATGCGGAGGC GTTTGCTTGG TGGTCGGACT TGATGGTGGA ACACGCAGAG
    ACCTTCCTTT CGCTTTTCGC GGTGGATATG GATGCTGCTT TAGAGGTGCA ACCGCCAGAC
    AGCTGGGACA GCTTCCCTCT TTTTCAGCTA ATAAATGACT TCCTTCGAAG TGACTATAAT
    TTATGCAATG GGAAATTCCA CAAACACCTT CAAGATCTCT TTGCTCCACT CGTTGTGAGA
    TACGTCGACC TCATGGAGTC CTCAATTGCA CAGTCAATTC ACAGGGGCTT TGAGCGTGAA
    TCATGGGAAC CAGTAAACAA TGGATCAGCA ACTTCGGAAG ATCTATTCTG GAAGCTTGAC
    GCTCTGCAAA CGTTCATTCG AGATCTTCAT TGGCCCGAGG AGGAATTCGG GAAACACCTA
    GAGCAACGCC TTAAACTTAT GGCTAGCGAT ATGATCGAAT CGTGTGTCAA ACGAACTAGA
    ATTGCATTCG AAGTCAAACT GCAGAAAACC AGTCGATCAA CAGACTTCCG AGTACCGCAA
    TCAATATGCA CCATGTTTAA TGTTATGGTT GATGCCAAAG CTCAGTCAAC GAAACTTTGT
    AGCATGGAAA TGGGCCAAGA GCACCAGTAC CACTCCCAAA TTGACGAGCT AATTGAAGAA
    ACAGTTAAAG AGATGATCAC ACTCTTAGTG GCAAAGTTTG TCACAATTCT AGAAGGAGTT
    CTGTCCAAAC TATCCAGATA CGATGAAGGG ACATTATTTT CCTCCTTTCT GTCATTTACT
    GTGAAAGCAG CTTCCAAGTA TGTGGATGTA CCAAAACCCG GGATGGACCT TGCCGACGCC
    TACGTGACAT TTGTGCGCCA CTCTCAGGAT GTCCTGCGTG ATAAGGTCAA TGAGGAGATA
    TATATAGAAA GGTTATTTGA TCAATGGTAT ACCAGCTCAA TGAATGTTGT TTGTACTTGG
    CTGACCGACC GTATGGATCT GCAACTTCAC ATTTATCAGC TGAAAACCCT AATTAGGGTC
    GTCAAGAAAA CCTACCGCGA TTTCCGATTG CAAGGCGTCT TGGACTCCAC CTTGAACAGC
    AAGACCTACG ACACCATTAG AAACCGCCTC ACCGTGGAAG AAGCCACAGC GTCCGTGAGC
    GAGGGGGGAG GCCTCCAGGG AATCACCATG AAGGACAGCG ACGAGGAGGA TGAGGAGGAT
    GATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55656
    Clone ID Related Accession (Same CDS sequence) XM_012960436.3
    Accession Version XM_012960436.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3825bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012960436.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTATG CTGATAGGGC ACAAAAACAT GGCATGGATG AATTTATCTC GTCCAATCCC
    TGTAACTTTG ACCACGCCAC CCTCTTTGAG ATGGTTCAGC GACTCACACT GGATCACAGA
    CTCAACGACT CGTACTCATG TCTGGGTTGG TTCAGTCCAG GTCAGGTCTT TGTACTCGAT
    GAATATTGTG CAAGATATGG AGTCCGAGGC TGCCATAGGC ATTTGTGTTA CCTTGGTGAT
    TTACTAGAAC GAGCAGAGAA TGGGTCAATG GTCGACCCAA CTTTACTTCA CTATAGTTTT
    GCCTTCTGCG CATCCCACGT ACATGGCAAC AGGCCTGATG GCATCGGAAC TGTAACCGTG
    GAAGAAAAAG AGCGCTTTGA AGAGATCAAA GAACGTCTCC GCTTGCTTTT GGAGAATCAG
    ATCACGCATT TTAGGTACTG CTTTCCATTT GGCCGACCAG AAGGTGCTTT GAAAGCCACT
    CTGTCATTGC TGGAAAGGGT TTTAATGAAA GACATAGTAA CTCCCGTCCC TCAAGAAGAA
    GTGAAAAACG TTATCCGTAA ATGCTTAGAG CAAGCAGCCT TAGTTAACTA CACGAGATTA
    TCCGAATATG CCAAAATCGA AGAAAATGTA GGCCGATTAG TAACGCCTGC CAAAAAGCTC
    GAAGATACAA TACGGCTCGC AGAACTTGTC ATTGAAGTTC TACAGCAGAA TGAGGAGCAC
    CATGCGGAGG CGTTTGCTTG GTGGTCGGAC TTGATGGTGG AACACGCAGA GACCTTCCTT
    TCGCTTTTCG CGGTGGATAT GGATGCTGCT TTAGAGGTGC AACCGCCAGA CAGCTGGGAC
    AGCTTCCCTC TTTTTCAGCT AATAAATGAC TTCCTTCGAA GTGACTATAA TTTATGCAAT
    GGGAAATTCC ACAAACACCT TCAAGATCTC TTTGCTCCAC TCGTTGTGAG ATACGTCGAC
    CTCATGGAGT CCTCAATTGC ACAGTCAATT CACAGGGGCT TTGAGCGTGA ATCATGGGAA
    CCAGTAAACA ATGGATCAGC AACTTCGGAA GATCTATTCT GGAAGCTTGA CGCTCTGCAA
    ACGTTCATTC GAGATCTTCA TTGGCCCGAG GAGGAATTCG GGAAACACCT AGAGCAACGC
    CTTAAACTTA TGGCTAGCGA TATGATCGAA TCGTGTGTCA AACGAACTAG AATTGCATTC
    GAAGTCAAAC TGCAGAAAAC CAGTCGATCA ACAGACTTCC GAGTACCGCA ATCAATATGC
    ACCATGTTTA ATGTTATGGT TGATGCCAAA GCTCAGTCAA CGAAACTTTG TAGCATGGAA
    ATGGGCCAAG AGCACCAGTA CCACTCCCAA ATTGACGAGC TAATTGAAGA AACAGTTAAA
    GAGATGATCA CACTCTTAGT GGCAAAGTTT GTCACAATTC TAGAAGGAGT TCTGTCCAAA
    CTATCCAGAT ACGATGAAGG GACATTATTT TCCTCCTTTC TGTCATTTAC TGTGAAAGCA
    GCTTCCAAGT ATGTGGATGT ACCAAAACCC GGGATGGACC TTGCCGACGC CTACGTGACA
    TTTGTGCGCC ACTCTCAGGA TGTCCTGCGT GATAAGGTCA ATGAGGAGAT ATATATAGAA
    AGGTTATTTG ATAAAAGGCT GGATGGTAAT AGTTCTGTCA TGTACCTGAG AATATTGGAG
    CAATGGTATA CCAGCTCAAT GAATGTTGTT TGTACTTGGC TGACCGACCG TATGGATCTG
    CAACTTCACA TTTATCAGCT GAAAACCCTA ATTAGGGTCG TCAAGAAAAC CTACCGCGAT
    TTCCGATTGC AAGGCGTCTT GGACTCCACC TTGAACAGCA AGACCTACGA CACCATTAGA
    AACCGCCTCA CCGTGGAAGA AGCCACAGCG TCCGTGAGCG AGGGGGGAGG CCTCCAGGGA
    ATCACCATGA AGGACAGCGA CGAGGAGGAT GAGGAGGATG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815890.1
    CDS595..4419
    Translation

    Target ORF information:

    RefSeq Version XM_012960436.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012960436.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTATG CTGATAGGGC ACAAAAACAT GGCATGGATG AATTTATCTC GTCCAATCCC
    TGTAACTTTG ACCACGCCAC CCTCTTTGAG ATGGTTCAGC GACTCACACT GGATCACAGA
    CTCAACGACT CGTACTCATG TCTGGGTTGG TTCAGTCCAG GTCAGGTCTT TGTACTCGAT
    GAATATTGTG CAAGATATGG AGTCCGAGGC TGCCATAGGC ATTTGTGTTA CCTTGGTGAT
    TTACTAGAAC GAGCAGAGAA TGGGTCAATG GTCGACCCAA CTTTACTTCA CTATAGTTTT
    GCCTTCTGCG CATCCCACGT ACATGGCAAC AGGCCTGATG GCATCGGAAC TGTAACCGTG
    GAAGAAAAAG AGCGCTTTGA AGAGATCAAA GAACGTCTCC GCTTGCTTTT GGAGAATCAG
    ATCACGCATT TTAGGTACTG CTTTCCATTT GGCCGACCAG AAGGTGCTTT GAAAGCCACT
    CTGTCATTGC TGGAAAGGGT TTTAATGAAA GACATAGTAA CTCCCGTCCC TCAAGAAGAA
    GTGAAAAACG TTATCCGTAA ATGCTTAGAG CAAGCAGCCT TAGTTAACTA CACGAGATTA
    TCCGAATATG CCAAAATCGA AGAAAATGTA GGCCGATTAG TAACGCCTGC CAAAAAGCTC
    GAAGATACAA TACGGCTCGC AGAACTTGTC ATTGAAGTTC TACAGCAGAA TGAGGAGCAC
    CATGCGGAGG CGTTTGCTTG GTGGTCGGAC TTGATGGTGG AACACGCAGA GACCTTCCTT
    TCGCTTTTCG CGGTGGATAT GGATGCTGCT TTAGAGGTGC AACCGCCAGA CAGCTGGGAC
    AGCTTCCCTC TTTTTCAGCT AATAAATGAC TTCCTTCGAA GTGACTATAA TTTATGCAAT
    GGGAAATTCC ACAAACACCT TCAAGATCTC TTTGCTCCAC TCGTTGTGAG ATACGTCGAC
    CTCATGGAGT CCTCAATTGC ACAGTCAATT CACAGGGGCT TTGAGCGTGA ATCATGGGAA
    CCAGTAAACA ATGGATCAGC AACTTCGGAA GATCTATTCT GGAAGCTTGA CGCTCTGCAA
    ACGTTCATTC GAGATCTTCA TTGGCCCGAG GAGGAATTCG GGAAACACCT AGAGCAACGC
    CTTAAACTTA TGGCTAGCGA TATGATCGAA TCGTGTGTCA AACGAACTAG AATTGCATTC
    GAAGTCAAAC TGCAGAAAAC CAGTCGATCA ACAGACTTCC GAGTACCGCA ATCAATATGC
    ACCATGTTTA ATGTTATGGT TGATGCCAAA GCTCAGTCAA CGAAACTTTG TAGCATGGAA
    ATGGGCCAAG AGCACCAGTA CCACTCCCAA ATTGACGAGC TAATTGAAGA AACAGTTAAA
    GAGATGATCA CACTCTTAGT GGCAAAGTTT GTCACAATTC TAGAAGGAGT TCTGTCCAAA
    CTATCCAGAT ACGATGAAGG GACATTATTT TCCTCCTTTC TGTCATTTAC TGTGAAAGCA
    GCTTCCAAGT ATGTGGATGT ACCAAAACCC GGGATGGACC TTGCCGACGC CTACGTGACA
    TTTGTGCGCC ACTCTCAGGA TGTCCTGCGT GATAAGGTCA ATGAGGAGAT ATATATAGAA
    AGGTTATTTG ATAAAAGGCT GGATGGTAAT AGTTCTGTCA TGTACCTGAG AATATTGGAG
    CAATGGTATA CCAGCTCAAT GAATGTTGTT TGTACTTGGC TGACCGACCG TATGGATCTG
    CAACTTCACA TTTATCAGCT GAAAACCCTA ATTAGGGTCG TCAAGAAAAC CTACCGCGAT
    TTCCGATTGC AAGGCGTCTT GGACTCCACC TTGAACAGCA AGACCTACGA CACCATTAGA
    AACCGCCTCA CCGTGGAAGA AGCCACAGCG TCCGTGAGCG AGGGGGGAGG CCTCCAGGGA
    ATCACCATGA AGGACAGCGA CGAGGAGGAT GAGGAGGATG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55657
    Clone ID Related Accession (Same CDS sequence) XM_012960437.3
    Accession Version XM_012960437.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012960437.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGAAAATG TAGGCCGATT AGTAACGCCT
    GCCAAAAAGC TCGAAGATAC AATACGGCTC GCAGAACTTG TCATTGAAGT TCTACAGCAG
    AATGAGGAGC ACCATGCGGA GGCGTTTGCT TGGTGGTCGG ACTTGATGGT GGAACACGCA
    GAGACCTTCC TTTCGCTTTT CGCGGTGGAT ATGGATGCTG CTTTAGAGGT GCAACCGCCA
    GACAGCTGGG ACAGCTTCCC TCTTTTTCAG CTAATAAATG ACTTCCTTCG AAGTGACTAT
    AATTTATGCA ATGGGAAATT CCACAAACAC CTTCAAGATC TCTTTGCTCC ACTCGTTGTG
    AGATACGTCG ACCTCATGGA GTCCTCAATT GCACAGTCAA TTCACAGGGG CTTTGAGCGT
    GAATCATGGG AACCAGTAAA CAATGGATCA GCAACTTCGG AAGATCTATT CTGGAAGCTT
    GACGCTCTGC AAACGTTCAT TCGAGATCTT CATTGGCCCG AGGAGGAATT CGGGAAACAC
    CTAGAGCAAC GCCTTAAACT TATGGCTAGC GATATGATCG AATCGTGTGT CAAACGAACT
    AGAATTGCAT TCGAAGTCAA ACTGCAGAAA ACCAGTCGAT CAACAGACTT CCGAGTACCG
    CAATCAATAT GCACCATGTT TAATGTTATG GTTGATGCCA AAGCTCAGTC AACGAAACTT
    TGTAGCATGG AAATGGGCCA AGAGCACCAG TACCACTCCC AAATTGACGA GCTAATTGAA
    GAAACAGTTA AAGAGATGAT CACACTCTTA GTGGCAAAGT TTGTCACAAT TCTAGAAGGA
    GTTCTGTCCA AACTATCCAG ATACGATGAA GGGACATTAT TTTCCTCCTT TCTGTCATTT
    ACTGTGAAAG CAGCTTCCAA GTATGTGGAT GTACCAAAAC CCGGGATGGA CCTTGCCGAC
    GCCTACGTGA CATTTGTGCG CCACTCTCAG GATGTCCTGC GTGATAAGGT CAATGAGGAG
    ATATATATAG AAAGGTTATT TGATCAATGG TATACCAGCT CAATGAATGT TGTTTGTACT
    TGGCTGACCG ACCGTATGGA TCTGCAACTT CACATTTATC AGCTGAAAAC CCTAATTAGG
    GTCGTCAAGA AAACCTACCG CGATTTCCGA TTGCAAGGCG TCTTGGACTC CACCTTGAAC
    AGCAAGACCT ACGACACCAT TAGAAACCGC CTCACCGTGG AAGAAGCCAC AGCGTCCGTG
    AGCGAGGGGG GAGGCCTCCA GGGAATCACC ATGAAGGACA GCGACGAGGA GGATGAGGAG
    GATGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815891.1
    CDS595..4383
    Translation

    Target ORF information:

    RefSeq Version XM_012960437.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012960437.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGAAAATG TAGGCCGATT AGTAACGCCT
    GCCAAAAAGC TCGAAGATAC AATACGGCTC GCAGAACTTG TCATTGAAGT TCTACAGCAG
    AATGAGGAGC ACCATGCGGA GGCGTTTGCT TGGTGGTCGG ACTTGATGGT GGAACACGCA
    GAGACCTTCC TTTCGCTTTT CGCGGTGGAT ATGGATGCTG CTTTAGAGGT GCAACCGCCA
    GACAGCTGGG ACAGCTTCCC TCTTTTTCAG CTAATAAATG ACTTCCTTCG AAGTGACTAT
    AATTTATGCA ATGGGAAATT CCACAAACAC CTTCAAGATC TCTTTGCTCC ACTCGTTGTG
    AGATACGTCG ACCTCATGGA GTCCTCAATT GCACAGTCAA TTCACAGGGG CTTTGAGCGT
    GAATCATGGG AACCAGTAAA CAATGGATCA GCAACTTCGG AAGATCTATT CTGGAAGCTT
    GACGCTCTGC AAACGTTCAT TCGAGATCTT CATTGGCCCG AGGAGGAATT CGGGAAACAC
    CTAGAGCAAC GCCTTAAACT TATGGCTAGC GATATGATCG AATCGTGTGT CAAACGAACT
    AGAATTGCAT TCGAAGTCAA ACTGCAGAAA ACCAGTCGAT CAACAGACTT CCGAGTACCG
    CAATCAATAT GCACCATGTT TAATGTTATG GTTGATGCCA AAGCTCAGTC AACGAAACTT
    TGTAGCATGG AAATGGGCCA AGAGCACCAG TACCACTCCC AAATTGACGA GCTAATTGAA
    GAAACAGTTA AAGAGATGAT CACACTCTTA GTGGCAAAGT TTGTCACAAT TCTAGAAGGA
    GTTCTGTCCA AACTATCCAG ATACGATGAA GGGACATTAT TTTCCTCCTT TCTGTCATTT
    ACTGTGAAAG CAGCTTCCAA GTATGTGGAT GTACCAAAAC CCGGGATGGA CCTTGCCGAC
    GCCTACGTGA CATTTGTGCG CCACTCTCAG GATGTCCTGC GTGATAAGGT CAATGAGGAG
    ATATATATAG AAAGGTTATT TGATCAATGG TATACCAGCT CAATGAATGT TGTTTGTACT
    TGGCTGACCG ACCGTATGGA TCTGCAACTT CACATTTATC AGCTGAAAAC CCTAATTAGG
    GTCGTCAAGA AAACCTACCG CGATTTCCGA TTGCAAGGCG TCTTGGACTC CACCTTGAAC
    AGCAAGACCT ACGACACCAT TAGAAACCGC CTCACCGTGG AAGAAGCCAC AGCGTCCGTG
    AGCGAGGGGG GAGGCCTCCA GGGAATCACC ATGAAGGACA GCGACGAGGA GGATGAGGAG
    GATGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55658
    Clone ID Related Accession (Same CDS sequence) XM_031899730.1
    Accession Version XM_031899730.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3786bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X23
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTATG CTGATAGGGC ACAAAAACAT GGCATGGATG AATTTATCTC GTCCAATCCC
    TGTAACTTTG ACCACGCCAC CCTCTTTGAG ATGGTTCAGC GACTCACACT GGATCACAGA
    CTCAACGACT CGTACTCATG TCTGGGTTGG TTCAGTCCAG GTCAGGTCTT TGTACTCGAT
    GAATATTGTG CAAGATATGG AGTCCGAGGC TGCCATAGGC ATTTGTGTTA CCTTGGTGAT
    TTACTAGAAC GAGCAGAGAA TGGGTCAATG GTCGACCCAA CTTTACTTCA CTATAGTTTT
    GCCTTCTGCG CATCCCACGT ACATGGCAAC AGGCCTGATG GCATCGGAAC TGTAACCGTG
    GAAGAAAAAG AGCGCTTTGA AGAGATCAAA GAACGTCTCC GCTTGCTTTT GGAGAATCAG
    ATCACGCATT TTAGGTACTG CTTTCCATTT GGCCGACCAG AAGGTGCTTT GAAAGCCACT
    CTGTCATTGC TGGAAAGGGT TTTAATGAAA GACATAGTAA CTCCCGTCCC TCAAGAAGAA
    GTGAAAAACG TTATCCGTAA ATGCTTAGAG CAAGCAGCCT TAGTTAACTA CACGAGATTA
    TCCGAATATG CCAAAATCGA AGAAAATGTA GGCCGATTAG TAACGCCTGC CAAAAAGCTC
    GAAGATACAA TACGGCTCGC AGAACTTGTC ATTGAAGTTC TACAGCAGAA TGAGGAGCAC
    CATGCGGAGG GGAAAGAGGC GTTTGCTTGG TGGTCGGACT TGATGGTGGA ACACGCAGAG
    ACCTTCCTTT CGCTTTTCGC GGTGGATATG GATGCTGCTT TAGAGGTGCA ACCGCCAGAC
    AGCTGGGACA GCTTCCCTCT TTTTCAGCTA ATAAATGACT TCCTTCGAAG TGACTATAAT
    TTATGCAATG GGAAATTCCA CAAACACCTT CAAGATCTCT TTGCTCCACT CGTTGTGAGA
    TACGTCGACC TCATGGAGTC CTCAATTGCA CAGTCAATTC ACAGGGGCTT TGAGCGTGAA
    TCATGGGAAC CAGTAAACAA TGGATCAGCA ACTTCGGAAG ATCTATTCTG GAAGCTTGAC
    GCTCTGCAAA CGTTCATTCG AGATCTTCAT TGGCCCGAGG AGGAATTCGG GAAACACCTA
    GAGCAACGCC TTAAACTTAT GGCTAGCGAT ATGATCGAAT CGTGTGTCAA ACGAACTAGA
    ATTGCATTCG AAGTCAAACT GCAGAAAACC AGTCGATCAA CAGACTTCCG AGTACCGCAA
    TCAATATGCA CCATGTTTAA TGTTATGGTT GATGCCAAAG CTCAGTCAAC GAAACTTTGT
    AGCATGGAAA TGGGCCAAGA GCACCAGTAC CACTCCCAAA TTGACGAGCT AATTGAAGAA
    ACAGTTAAAG AGATGATCAC ACTCTTAGTG GCAAAGTTTG TCACAATTCT AGAAGGAGTT
    CTGTCCAAAC TATCCAGATA CGATGAAGGG ACATTATTTT CCTCCTTTCT GTCATTTACT
    GTGAAAGCAG CTTCCAAGTA TGTGGATGTA CCAAAACCCG GGATGGACCT TGCCGACGCC
    TACGTGACAT TTGTGCGCCA CTCTCAGGAT GTCCTGCGTG ATAAGGTCAA TGAGGAGATA
    TATATAGAAA GGTTATTTGA TCAATGGTAT ACCAGCTCAA TGAATGTTGT TTGTACTTGG
    CTGACCGACC GTATGGATCT GCAACTTCAC ATTTATCAGC TGAAAACCCT AATTAGGGTC
    GTCAAGAAAA CCTACCGCGA TTTCCGATTG CAAGGCGTCT TGGACTCCAC CTTGAACAGC
    AAGACCTACG ACACCATTAG AAACCGCCTC ACCGTGGAAG AAGCCACAGC GTCCGTGAGC
    GAGGGGGGAG GCCTCCAGGG AATCACCATG AAGGACAGCG ACGAGGAGGA TGAGGAGGAT
    GATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031755590.1
    CDS593..4378
    Translation

    Target ORF information:

    RefSeq Version XM_031899730.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899730.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTATG CTGATAGGGC ACAAAAACAT GGCATGGATG AATTTATCTC GTCCAATCCC
    TGTAACTTTG ACCACGCCAC CCTCTTTGAG ATGGTTCAGC GACTCACACT GGATCACAGA
    CTCAACGACT CGTACTCATG TCTGGGTTGG TTCAGTCCAG GTCAGGTCTT TGTACTCGAT
    GAATATTGTG CAAGATATGG AGTCCGAGGC TGCCATAGGC ATTTGTGTTA CCTTGGTGAT
    TTACTAGAAC GAGCAGAGAA TGGGTCAATG GTCGACCCAA CTTTACTTCA CTATAGTTTT
    GCCTTCTGCG CATCCCACGT ACATGGCAAC AGGCCTGATG GCATCGGAAC TGTAACCGTG
    GAAGAAAAAG AGCGCTTTGA AGAGATCAAA GAACGTCTCC GCTTGCTTTT GGAGAATCAG
    ATCACGCATT TTAGGTACTG CTTTCCATTT GGCCGACCAG AAGGTGCTTT GAAAGCCACT
    CTGTCATTGC TGGAAAGGGT TTTAATGAAA GACATAGTAA CTCCCGTCCC TCAAGAAGAA
    GTGAAAAACG TTATCCGTAA ATGCTTAGAG CAAGCAGCCT TAGTTAACTA CACGAGATTA
    TCCGAATATG CCAAAATCGA AGAAAATGTA GGCCGATTAG TAACGCCTGC CAAAAAGCTC
    GAAGATACAA TACGGCTCGC AGAACTTGTC ATTGAAGTTC TACAGCAGAA TGAGGAGCAC
    CATGCGGAGG GGAAAGAGGC GTTTGCTTGG TGGTCGGACT TGATGGTGGA ACACGCAGAG
    ACCTTCCTTT CGCTTTTCGC GGTGGATATG GATGCTGCTT TAGAGGTGCA ACCGCCAGAC
    AGCTGGGACA GCTTCCCTCT TTTTCAGCTA ATAAATGACT TCCTTCGAAG TGACTATAAT
    TTATGCAATG GGAAATTCCA CAAACACCTT CAAGATCTCT TTGCTCCACT CGTTGTGAGA
    TACGTCGACC TCATGGAGTC CTCAATTGCA CAGTCAATTC ACAGGGGCTT TGAGCGTGAA
    TCATGGGAAC CAGTAAACAA TGGATCAGCA ACTTCGGAAG ATCTATTCTG GAAGCTTGAC
    GCTCTGCAAA CGTTCATTCG AGATCTTCAT TGGCCCGAGG AGGAATTCGG GAAACACCTA
    GAGCAACGCC TTAAACTTAT GGCTAGCGAT ATGATCGAAT CGTGTGTCAA ACGAACTAGA
    ATTGCATTCG AAGTCAAACT GCAGAAAACC AGTCGATCAA CAGACTTCCG AGTACCGCAA
    TCAATATGCA CCATGTTTAA TGTTATGGTT GATGCCAAAG CTCAGTCAAC GAAACTTTGT
    AGCATGGAAA TGGGCCAAGA GCACCAGTAC CACTCCCAAA TTGACGAGCT AATTGAAGAA
    ACAGTTAAAG AGATGATCAC ACTCTTAGTG GCAAAGTTTG TCACAATTCT AGAAGGAGTT
    CTGTCCAAAC TATCCAGATA CGATGAAGGG ACATTATTTT CCTCCTTTCT GTCATTTACT
    GTGAAAGCAG CTTCCAAGTA TGTGGATGTA CCAAAACCCG GGATGGACCT TGCCGACGCC
    TACGTGACAT TTGTGCGCCA CTCTCAGGAT GTCCTGCGTG ATAAGGTCAA TGAGGAGATA
    TATATAGAAA GGTTATTTGA TCAATGGTAT ACCAGCTCAA TGAATGTTGT TTGTACTTGG
    CTGACCGACC GTATGGATCT GCAACTTCAC ATTTATCAGC TGAAAACCCT AATTAGGGTC
    GTCAAGAAAA CCTACCGCGA TTTCCGATTG CAAGGCGTCT TGGACTCCAC CTTGAACAGC
    AAGACCTACG ACACCATTAG AAACCGCCTC ACCGTGGAAG AAGCCACAGC GTCCGTGAGC
    GAGGGGGGAG GCCTCCAGGG AATCACCATG AAGGACAGCG ACGAGGAGGA TGAGGAGGAT
    GATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55659
    Clone ID Related Accession (Same CDS sequence) XM_012960442.3
    Accession Version XM_012960442.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3777bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X24
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012960442.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTATG CTGATAGGGC ACAAAAACAT GGCATGGATG AATTTATCTC GTCCAATCCC
    TGTAACTTTG ACCACGCCAC CCTCTTTGAG ATGGTTCAGC GACTCACACT GGATCACAGA
    CTCAACGACT CGTACTCATG TCTGGGTTGG TTCAGTCCAG GTCAGGTCTT TGTACTCGAT
    GAATATTGTG CAAGATATGG AGTCCGAGGC TGCCATAGGC ATTTGTGTTA CCTTGGTGAT
    TTACTAGAAC GAGCAGAGAA TGGGTCAATG GTCGACCCAA CTTTACTTCA CTATAGTTTT
    GCCTTCTGCG CATCCCACGT ACATGGCAAC AGGCCTGATG GCATCGGAAC TGTAACCGTG
    GAAGAAAAAG AGCGCTTTGA AGAGATCAAA GAACGTCTCC GCTTGCTTTT GGAGAATCAG
    ATCACGCATT TTAGGTACTG CTTTCCATTT GGCCGACCAG AAGGTGCTTT GAAAGCCACT
    CTGTCATTGC TGGAAAGGGT TTTAATGAAA GACATAGTAA CTCCCGTCCC TCAAGAAGAA
    GTGAAAAACG TTATCCGTAA ATGCTTAGAG CAAGCAGCCT TAGTTAACTA CACGAGATTA
    TCCGAATATG CCAAAATCGA AGAAAATGTA GGCCGATTAG TAACGCCTGC CAAAAAGCTC
    GAAGATACAA TACGGCTCGC AGAACTTGTC ATTGAAGTTC TACAGCAGAA TGAGGAGCAC
    CATGCGGAGG CGTTTGCTTG GTGGTCGGAC TTGATGGTGG AACACGCAGA GACCTTCCTT
    TCGCTTTTCG CGGTGGATAT GGATGCTGCT TTAGAGGTGC AACCGCCAGA CAGCTGGGAC
    AGCTTCCCTC TTTTTCAGCT AATAAATGAC TTCCTTCGAA GTGACTATAA TTTATGCAAT
    GGGAAATTCC ACAAACACCT TCAAGATCTC TTTGCTCCAC TCGTTGTGAG ATACGTCGAC
    CTCATGGAGT CCTCAATTGC ACAGTCAATT CACAGGGGCT TTGAGCGTGA ATCATGGGAA
    CCAGTAAACA ATGGATCAGC AACTTCGGAA GATCTATTCT GGAAGCTTGA CGCTCTGCAA
    ACGTTCATTC GAGATCTTCA TTGGCCCGAG GAGGAATTCG GGAAACACCT AGAGCAACGC
    CTTAAACTTA TGGCTAGCGA TATGATCGAA TCGTGTGTCA AACGAACTAG AATTGCATTC
    GAAGTCAAAC TGCAGAAAAC CAGTCGATCA ACAGACTTCC GAGTACCGCA ATCAATATGC
    ACCATGTTTA ATGTTATGGT TGATGCCAAA GCTCAGTCAA CGAAACTTTG TAGCATGGAA
    ATGGGCCAAG AGCACCAGTA CCACTCCCAA ATTGACGAGC TAATTGAAGA AACAGTTAAA
    GAGATGATCA CACTCTTAGT GGCAAAGTTT GTCACAATTC TAGAAGGAGT TCTGTCCAAA
    CTATCCAGAT ACGATGAAGG GACATTATTT TCCTCCTTTC TGTCATTTAC TGTGAAAGCA
    GCTTCCAAGT ATGTGGATGT ACCAAAACCC GGGATGGACC TTGCCGACGC CTACGTGACA
    TTTGTGCGCC ACTCTCAGGA TGTCCTGCGT GATAAGGTCA ATGAGGAGAT ATATATAGAA
    AGGTTATTTG ATCAATGGTA TACCAGCTCA ATGAATGTTG TTTGTACTTG GCTGACCGAC
    CGTATGGATC TGCAACTTCA CATTTATCAG CTGAAAACCC TAATTAGGGT CGTCAAGAAA
    ACCTACCGCG ATTTCCGATT GCAAGGCGTC TTGGACTCCA CCTTGAACAG CAAGACCTAC
    GACACCATTA GAAACCGCCT CACCGTGGAA GAAGCCACAG CGTCCGTGAG CGAGGGGGGA
    GGCCTCCAGG GAATCACCAT GAAGGACAGC GACGAGGAGG ATGAGGAGGA TGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815896.1
    CDS591..4367
    Translation

    Target ORF information:

    RefSeq Version XM_012960442.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012960442.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTATG CTGATAGGGC ACAAAAACAT GGCATGGATG AATTTATCTC GTCCAATCCC
    TGTAACTTTG ACCACGCCAC CCTCTTTGAG ATGGTTCAGC GACTCACACT GGATCACAGA
    CTCAACGACT CGTACTCATG TCTGGGTTGG TTCAGTCCAG GTCAGGTCTT TGTACTCGAT
    GAATATTGTG CAAGATATGG AGTCCGAGGC TGCCATAGGC ATTTGTGTTA CCTTGGTGAT
    TTACTAGAAC GAGCAGAGAA TGGGTCAATG GTCGACCCAA CTTTACTTCA CTATAGTTTT
    GCCTTCTGCG CATCCCACGT ACATGGCAAC AGGCCTGATG GCATCGGAAC TGTAACCGTG
    GAAGAAAAAG AGCGCTTTGA AGAGATCAAA GAACGTCTCC GCTTGCTTTT GGAGAATCAG
    ATCACGCATT TTAGGTACTG CTTTCCATTT GGCCGACCAG AAGGTGCTTT GAAAGCCACT
    CTGTCATTGC TGGAAAGGGT TTTAATGAAA GACATAGTAA CTCCCGTCCC TCAAGAAGAA
    GTGAAAAACG TTATCCGTAA ATGCTTAGAG CAAGCAGCCT TAGTTAACTA CACGAGATTA
    TCCGAATATG CCAAAATCGA AGAAAATGTA GGCCGATTAG TAACGCCTGC CAAAAAGCTC
    GAAGATACAA TACGGCTCGC AGAACTTGTC ATTGAAGTTC TACAGCAGAA TGAGGAGCAC
    CATGCGGAGG CGTTTGCTTG GTGGTCGGAC TTGATGGTGG AACACGCAGA GACCTTCCTT
    TCGCTTTTCG CGGTGGATAT GGATGCTGCT TTAGAGGTGC AACCGCCAGA CAGCTGGGAC
    AGCTTCCCTC TTTTTCAGCT AATAAATGAC TTCCTTCGAA GTGACTATAA TTTATGCAAT
    GGGAAATTCC ACAAACACCT TCAAGATCTC TTTGCTCCAC TCGTTGTGAG ATACGTCGAC
    CTCATGGAGT CCTCAATTGC ACAGTCAATT CACAGGGGCT TTGAGCGTGA ATCATGGGAA
    CCAGTAAACA ATGGATCAGC AACTTCGGAA GATCTATTCT GGAAGCTTGA CGCTCTGCAA
    ACGTTCATTC GAGATCTTCA TTGGCCCGAG GAGGAATTCG GGAAACACCT AGAGCAACGC
    CTTAAACTTA TGGCTAGCGA TATGATCGAA TCGTGTGTCA AACGAACTAG AATTGCATTC
    GAAGTCAAAC TGCAGAAAAC CAGTCGATCA ACAGACTTCC GAGTACCGCA ATCAATATGC
    ACCATGTTTA ATGTTATGGT TGATGCCAAA GCTCAGTCAA CGAAACTTTG TAGCATGGAA
    ATGGGCCAAG AGCACCAGTA CCACTCCCAA ATTGACGAGC TAATTGAAGA AACAGTTAAA
    GAGATGATCA CACTCTTAGT GGCAAAGTTT GTCACAATTC TAGAAGGAGT TCTGTCCAAA
    CTATCCAGAT ACGATGAAGG GACATTATTT TCCTCCTTTC TGTCATTTAC TGTGAAAGCA
    GCTTCCAAGT ATGTGGATGT ACCAAAACCC GGGATGGACC TTGCCGACGC CTACGTGACA
    TTTGTGCGCC ACTCTCAGGA TGTCCTGCGT GATAAGGTCA ATGAGGAGAT ATATATAGAA
    AGGTTATTTG ATCAATGGTA TACCAGCTCA ATGAATGTTG TTTGTACTTG GCTGACCGAC
    CGTATGGATC TGCAACTTCA CATTTATCAG CTGAAAACCC TAATTAGGGT CGTCAAGAAA
    ACCTACCGCG ATTTCCGATT GCAAGGCGTC TTGGACTCCA CCTTGAACAG CAAGACCTAC
    GACACCATTA GAAACCGCCT CACCGTGGAA GAAGCCACAG CGTCCGTGAG CGAGGGGGGA
    GGCCTCCAGG GAATCACCAT GAAGGACAGC GACGAGGAGG ATGAGGAGGA TGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55660
    Clone ID Related Accession (Same CDS sequence) XM_012960438.3
    Accession Version XM_012960438.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3954bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X25
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012960438.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGAGAATC AAAAGGACGC AGAAAATGTA
    GGCCGATTAG TAACGCCTGC CAAAAAGCTC GAAGATACAA TACGGCTCGC AGAACTTGTC
    ATTGAAGTTC TACAGCAGAA TGAGGAGCAC CATGCGGAGG GGAAAGAGGC GTTTGCTTGG
    TGGTCGGACT TGATGGTGGA ACACGCAGAG ACCTTCCTTT CGCTTTTCGC GGTGGATATG
    GATGCTGCTT TAGAGGTGCA ACCGCCAGAC AGCTGGGACA GCTTCCCTCT TTTTCAGCTA
    ATAAATGACT TCCTTCGAAG TGACTATAAT TTATGCAATG GGAAATTCCA CAAACACCTT
    CAAGATCTCT TTGCTCCACT CGTTGTGAGA TACGTCGACC TCATGGAGTC CTCAATTGCA
    CAGTCAATTC ACAGGGGCTT TGAGCGTGAA TCATGGGAAC CAGTAAAGAG TTTAACAAGT
    AATCTACCCA ATGTGAATCT ACCAAATGTG AATCTCCCTA AAGTACCAGT TACCTTACCA
    GTTAACCTCC CACAAATGCC TAGTTTTTCT GCACCGTCAT GGATGGCAGC TATATATGAT
    TCGGACAATG GATCAGCAAC TTCGGAAGAT CTATTCTGGA AGCTTGACGC TCTGCAAACG
    TTCATTCGAG ATCTTCATTG GCCCGAGGAG GAATTCGGGA AACACCTAGA GCAACGCCTT
    AAACTTATGG CTAGCGATAT GATCGAATCG TGTGTCAAAC GAACTAGAAT TGCATTCGAA
    GTCAAACTGC AGAAAACCAG TCGATCAACA GACTTCCGAG TACCGCAATC AATATGCACC
    ATGTTTAATG TTATGGTTGA TGCCAAAGCT CAGTCAACGA AACTTTGTAG CATGGAAATG
    GGCCAAGAGC ACCAGTACCA CTCCCAAATT GACGAGCTAA TTGAAGAAAC AGTTAAAGAG
    ATGATCACAC TCTTAGTGGC AAAGTTTGTC ACAATTCTAG AAGGAGTTCT GTCCAAACTA
    TCCAGATACG ATGAAGGGAC ATTATTTTCC TCCTTTCTGT CATTTACTGT GAAAGCAGCT
    TCCAAGTATG TGGATGTACC AAAACCCGGG ATGGACCTTG CCGACGCCTA CGTGACATTT
    GTGCGCCACT CTCAGGATGT CCTGCGTGAT AAGGTCAATG AGGAGATATA TATAGAAAGG
    TTATTTGATC AATGGTATAC CAGCTCAATG AATGTTGTTT GTACTTGGCT GACCGACCGT
    ATGGATCTGC AACTTCACAT TTATCAGCTG AAAACCCTAA TTAGGGTCGT CAAGAAAACC
    TACCGCGATT TCCGATTGCA AGGCGTCTTG GACTCCACCT TGAACAGCAA GACCTACGAC
    ACCATTAGAA ACCGCCTCAC CGTGGAAGAA GCCACAGCGT CCGTGAGCGA GGGGGGAGGC
    CTCCAGGGAA TCACCATGAA GGACAGCGAC GAGGAGGATG AGGAGGATGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815892.1
    CDS597..4550
    Translation

    Target ORF information:

    RefSeq Version XM_012960438.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X25, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012960438.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGAGAATC AAAAGGACGC AGAAAATGTA
    GGCCGATTAG TAACGCCTGC CAAAAAGCTC GAAGATACAA TACGGCTCGC AGAACTTGTC
    ATTGAAGTTC TACAGCAGAA TGAGGAGCAC CATGCGGAGG GGAAAGAGGC GTTTGCTTGG
    TGGTCGGACT TGATGGTGGA ACACGCAGAG ACCTTCCTTT CGCTTTTCGC GGTGGATATG
    GATGCTGCTT TAGAGGTGCA ACCGCCAGAC AGCTGGGACA GCTTCCCTCT TTTTCAGCTA
    ATAAATGACT TCCTTCGAAG TGACTATAAT TTATGCAATG GGAAATTCCA CAAACACCTT
    CAAGATCTCT TTGCTCCACT CGTTGTGAGA TACGTCGACC TCATGGAGTC CTCAATTGCA
    CAGTCAATTC ACAGGGGCTT TGAGCGTGAA TCATGGGAAC CAGTAAAGAG TTTAACAAGT
    AATCTACCCA ATGTGAATCT ACCAAATGTG AATCTCCCTA AAGTACCAGT TACCTTACCA
    GTTAACCTCC CACAAATGCC TAGTTTTTCT GCACCGTCAT GGATGGCAGC TATATATGAT
    TCGGACAATG GATCAGCAAC TTCGGAAGAT CTATTCTGGA AGCTTGACGC TCTGCAAACG
    TTCATTCGAG ATCTTCATTG GCCCGAGGAG GAATTCGGGA AACACCTAGA GCAACGCCTT
    AAACTTATGG CTAGCGATAT GATCGAATCG TGTGTCAAAC GAACTAGAAT TGCATTCGAA
    GTCAAACTGC AGAAAACCAG TCGATCAACA GACTTCCGAG TACCGCAATC AATATGCACC
    ATGTTTAATG TTATGGTTGA TGCCAAAGCT CAGTCAACGA AACTTTGTAG CATGGAAATG
    GGCCAAGAGC ACCAGTACCA CTCCCAAATT GACGAGCTAA TTGAAGAAAC AGTTAAAGAG
    ATGATCACAC TCTTAGTGGC AAAGTTTGTC ACAATTCTAG AAGGAGTTCT GTCCAAACTA
    TCCAGATACG ATGAAGGGAC ATTATTTTCC TCCTTTCTGT CATTTACTGT GAAAGCAGCT
    TCCAAGTATG TGGATGTACC AAAACCCGGG ATGGACCTTG CCGACGCCTA CGTGACATTT
    GTGCGCCACT CTCAGGATGT CCTGCGTGAT AAGGTCAATG AGGAGATATA TATAGAAAGG
    TTATTTGATC AATGGTATAC CAGCTCAATG AATGTTGTTT GTACTTGGCT GACCGACCGT
    ATGGATCTGC AACTTCACAT TTATCAGCTG AAAACCCTAA TTAGGGTCGT CAAGAAAACC
    TACCGCGATT TCCGATTGCA AGGCGTCTTG GACTCCACCT TGAACAGCAA GACCTACGAC
    ACCATTAGAA ACCGCCTCAC CGTGGAAGAA GCCACAGCGT CCGTGAGCGA GGGGGGAGGC
    CTCCAGGGAA TCACCATGAA GGACAGCGAC GAGGAGGATG AGGAGGATGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55661
    Clone ID Related Accession (Same CDS sequence) XM_012960441.3
    Accession Version XM_012960441.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3924bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X26
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012960441.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTATG CTGATAGGGC ACAAAAACAT GGCATGGATG AATTTATCTC GTCCAATCCC
    TGTAACTTTG ACCACGCCAC CCTCTTTGAG ATGGTTCAGC GACTCACACT GGATCACAGA
    CTCAACGACT CGTACTCATG TCTGGGTTGG TTCAGTCCAG GTCAGGTCTT TGTACTCGAT
    GAATATTGTG CAAGATATGG AGTCCGAGGC TGCCATAGGC ATTTGTGTTA CCTTGGTGAT
    TTACTAGAAC GAGCAGAGAA TGGGTCAATG GTCGACCCAA CTTTACTTCA CTATAGTTTT
    GCCTTCTGCG CATCCCACGT ACATGGCAAC AGGCCTGATG GCATCGGAAC TGTAACCGTG
    GAAGAAAAAG AGCGCTTTGA AGAGATCAAA GAACGTCTCC GCTTGCTTTT GGAGAATCAG
    ATCACGCATT TTAGGTACTG CTTTCCATTT GGCCGACCAG AAGGTGCTTT GAAAGCCACT
    CTGTCATTGC TGGAAAGGGT TTTAATGAAA GACATAGTAA CTCCCGTCCC TCAAGAAGAA
    GTGAAAAACG TTATCCGTAA ATGCTTAGAG CAAGCAGCCT TAGTTAACTA CACGAGATTA
    TCCGAATATG CCAAAATCGA AGAAAATGTA GGCCGATTAG TAACGCCTGC CAAAAAGCTC
    GAAGATACAA TACGGCTCGC AGAACTTGTC ATTGAAGTTC TACAGCAGAA TGAGGAGCAC
    CATGCGGAGG GGAAAGAGGC GTTTGCTTGG TGGTCGGACT TGATGGTGGA ACACGCAGAG
    ACCTTCCTTT CGCTTTTCGC GGTGGATATG GATGCTGCTT TAGAGGTGCA ACCGCCAGAC
    AGCTGGGACA GCTTCCCTCT TTTTCAGCTA ATAAATGACT TCCTTCGAAG TGACTATAAT
    TTATGCAATG GGAAATTCCA CAAACACCTT CAAGATCTCT TTGCTCCACT CGTTGTGAGA
    TACGTCGACC TCATGGAGTC CTCAATTGCA CAGTCAATTC ACAGGGGCTT TGAGCGTGAA
    TCATGGGAAC CAGTAAAGAG TTTAACAAGT AATCTACCCA ATGTGAATCT ACCAAATGTG
    AATCTCCCTA AAGTACCAGT TACCTTACCA GTTAACCTCC CACAAATGCC TAGTTTTTCT
    GCACCGTCAT GGATGGCAGC TATATATGAT TCGGACAATG GATCAGCAAC TTCGGAAGAT
    CTATTCTGGA AGCTTGACGC TCTGCAAACG TTCATTCGAG ATCTTCATTG GCCCGAGGAG
    GAATTCGGGA AACACCTAGA GCAACGCCTT AAACTTATGG CTAGCGATAT GATCGAATCG
    TGTGTCAAAC GAACTAGAAT TGCATTCGAA GTCAAACTGC AGAAAACCAG TCGATCAACA
    GACTTCCGAG TACCGCAATC AATATGCACC ATGTTTAATG TTATGGTTGA TGCCAAAGCT
    CAGTCAACGA AACTTTGTAG CATGGAAATG GGCCAAGAGC ACCAGTACCA CTCCCAAATT
    GACGAGCTAA TTGAAGAAAC AGTTAAAGAG ATGATCACAC TCTTAGTGGC AAAGTTTGTC
    ACAATTCTAG AAGGAGTTCT GTCCAAACTA TCCAGATACG ATGAAGGGAC ATTATTTTCC
    TCCTTTCTGT CATTTACTGT GAAAGCAGCT TCCAAGTATG TGGATGTACC AAAACCCGGG
    ATGGACCTTG CCGACGCCTA CGTGACATTT GTGCGCCACT CTCAGGATGT CCTGCGTGAT
    AAGGTCAATG AGGAGATATA TATAGAAAGG TTATTTGATC AATGGTATAC CAGCTCAATG
    AATGTTGTTT GTACTTGGCT GACCGACCGT ATGGATCTGC AACTTCACAT TTATCAGCTG
    AAAACCCTAA TTAGGGTCGT CAAGAAAACC TACCGCGATT TCCGATTGCA AGGCGTCTTG
    GACTCCACCT TGAACAGCAA GACCTACGAC ACCATTAGAA ACCGCCTCAC CGTGGAAGAA
    GCCACAGCGT CCGTGAGCGA GGGGGGAGGC CTCCAGGGAA TCACCATGAA GGACAGCGAC
    GAGGAGGATG AGGAGGATGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815895.1
    CDS595..4518
    Translation

    Target ORF information:

    RefSeq Version XM_012960441.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca2+ dependent secretion activator (cadps), transcript variant X26, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012960441.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATTGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTATG CTGATAGGGC ACAAAAACAT GGCATGGATG AATTTATCTC GTCCAATCCC
    TGTAACTTTG ACCACGCCAC CCTCTTTGAG ATGGTTCAGC GACTCACACT GGATCACAGA
    CTCAACGACT CGTACTCATG TCTGGGTTGG TTCAGTCCAG GTCAGGTCTT TGTACTCGAT
    GAATATTGTG CAAGATATGG AGTCCGAGGC TGCCATAGGC ATTTGTGTTA CCTTGGTGAT
    TTACTAGAAC GAGCAGAGAA TGGGTCAATG GTCGACCCAA CTTTACTTCA CTATAGTTTT
    GCCTTCTGCG CATCCCACGT ACATGGCAAC AGGCCTGATG GCATCGGAAC TGTAACCGTG
    GAAGAAAAAG AGCGCTTTGA AGAGATCAAA GAACGTCTCC GCTTGCTTTT GGAGAATCAG
    ATCACGCATT TTAGGTACTG CTTTCCATTT GGCCGACCAG AAGGTGCTTT GAAAGCCACT
    CTGTCATTGC TGGAAAGGGT TTTAATGAAA GACATAGTAA CTCCCGTCCC TCAAGAAGAA
    GTGAAAAACG TTATCCGTAA ATGCTTAGAG CAAGCAGCCT TAGTTAACTA CACGAGATTA
    TCCGAATATG CCAAAATCGA AGAAAATGTA GGCCGATTAG TAACGCCTGC CAAAAAGCTC
    GAAGATACAA TACGGCTCGC AGAACTTGTC ATTGAAGTTC TACAGCAGAA TGAGGAGCAC
    CATGCGGAGG GGAAAGAGGC GTTTGCTTGG TGGTCGGACT TGATGGTGGA ACACGCAGAG
    ACCTTCCTTT CGCTTTTCGC GGTGGATATG GATGCTGCTT TAGAGGTGCA ACCGCCAGAC
    AGCTGGGACA GCTTCCCTCT TTTTCAGCTA ATAAATGACT TCCTTCGAAG TGACTATAAT
    TTATGCAATG GGAAATTCCA CAAACACCTT CAAGATCTCT TTGCTCCACT CGTTGTGAGA
    TACGTCGACC TCATGGAGTC CTCAATTGCA CAGTCAATTC ACAGGGGCTT TGAGCGTGAA
    TCATGGGAAC CAGTAAAGAG TTTAACAAGT AATCTACCCA ATGTGAATCT ACCAAATGTG
    AATCTCCCTA AAGTACCAGT TACCTTACCA GTTAACCTCC CACAAATGCC TAGTTTTTCT
    GCACCGTCAT GGATGGCAGC TATATATGAT TCGGACAATG GATCAGCAAC TTCGGAAGAT
    CTATTCTGGA AGCTTGACGC TCTGCAAACG TTCATTCGAG ATCTTCATTG GCCCGAGGAG
    GAATTCGGGA AACACCTAGA GCAACGCCTT AAACTTATGG CTAGCGATAT GATCGAATCG
    TGTGTCAAAC GAACTAGAAT TGCATTCGAA GTCAAACTGC AGAAAACCAG TCGATCAACA
    GACTTCCGAG TACCGCAATC AATATGCACC ATGTTTAATG TTATGGTTGA TGCCAAAGCT
    CAGTCAACGA AACTTTGTAG CATGGAAATG GGCCAAGAGC ACCAGTACCA CTCCCAAATT
    GACGAGCTAA TTGAAGAAAC AGTTAAAGAG ATGATCACAC TCTTAGTGGC AAAGTTTGTC
    ACAATTCTAG AAGGAGTTCT GTCCAAACTA TCCAGATACG ATGAAGGGAC ATTATTTTCC
    TCCTTTCTGT CATTTACTGT GAAAGCAGCT TCCAAGTATG TGGATGTACC AAAACCCGGG
    ATGGACCTTG CCGACGCCTA CGTGACATTT GTGCGCCACT CTCAGGATGT CCTGCGTGAT
    AAGGTCAATG AGGAGATATA TATAGAAAGG TTATTTGATC AATGGTATAC CAGCTCAATG
    AATGTTGTTT GTACTTGGCT GACCGACCGT ATGGATCTGC AACTTCACAT TTATCAGCTG
    AAAACCCTAA TTAGGGTCGT CAAGAAAACC TACCGCGATT TCCGATTGCA AGGCGTCTTG
    GACTCCACCT TGAACAGCAA GACCTACGAC ACCATTAGAA ACCGCCTCAC CGTGGAAGAA
    GCCACAGCGT CCGTGAGCGA GGGGGGAGGC CTCCAGGGAA TCACCATGAA GGACAGCGAC
    GAGGAGGATG AGGAGGATGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa23892
    Clone ID Related Accession (Same CDS sequence) NM_001102712.1
    Accession Version NM_001102712.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3921bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC136018.1. ##Evidence-Data-START## Transcript exon combination :: BC136018.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028300, SAMD00028303 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACTATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGAGAATC AAAAGGACGC AGAAAATGTA
    GGCCGATTAG TAACGCCTGC CAAAAAGCTG GAAGATACAA TACGGCTCGC AGAACTTGTC
    ATTGAAGTTC TACAGCAGAA TGAGGAGCAC CATGCGGAGG GGAAAGAGGC GTTTGCTTGG
    TGGTCGGACT TGATGGTGGA ACACGCAGAG ACCTTCCTTT CGCTTTTCGC GGTGGATATG
    GATGCTGCTT TAGAGGTGCA ACCGCCAGAC AGCTGGGACA GCTTCCCTCT TTTTCAGCTA
    ATAAATGACT TCCTTCGAAG TGACTATAAT TTATGCAATG GGAAATTCCA CAAACACCTT
    CAAGATCTTT TTGCTCCACT CGTTGTGAGA TACGTCGACC TCATGGAGTC CTCAATTGCA
    CAGTCAATTC ACAGGGGCTT TGAGCGTGAA TCATGGGAAC CAGTAAAGAG TTTAACAAGT
    AATCTACCCA ATGTGAATCT ACCAAATGTG AATCTCCCTA AAGTACCAGT TACCTTACCA
    GTTAACCTCC CACAAATGCC TAGTTTTTCT GCACCGTCAT GGATGGCAGC TATATATGAT
    TCGGACAATG GATCAGCAAC TTCGGAAGAT CTATTCTGGA AGCTTGACGC TCTGCAAACG
    TTCATTCGAG ATCTTCATTG GCCCGAGGAG GAATTCGGGA AACACCTAGA GCAACGCCTT
    AAACTTATGG CTAGCGATAT GATCGAATCG TGTGTCAAAC GAACTAGAAT TGCATTCGAA
    GTCAAACTGC AGAAAACCAG TCGATCAACA GACTTCCGAG TACCGCAATC AATATGCACC
    ATGTTTAATG TTATGGTTGA TGCCAAAGCT CAGTCAACGA AACTTTGTAG CATGGAAATG
    GGCCAAGAGC ACCAGTACCA CTCCCAAATT GACGAGCTAA TTGAAGAAAC AGTTAAAGAG
    ATGATCACAC TCTTAGTGGC AAAGTTTGTC ACAATTCTAG AAGGAGTTCT GTCCAAACTA
    TCCAGATACG ATGAAGGGAC ATTATTTTCC TCCTTTCTGT CATTTACTAA ACCCGGGATG
    GACCTTGCCG ACGCCTACGT GACATTTGTG CGCCACTCTC AGGATGTCCT GCGTGATAAG
    GTCAATGAGG AGATATATAT AGAAAGGTTA TTTGATCAAT GGTATACCAG CTCAATGAAT
    GTTGTTTGTA CTTGGCTGAC CGACCGTATG GATCTGCAAC TTCACATTTA TCAGCTGAAA
    ACCCTAATTA GGGTCGTCAA GAAAACCTAC CGCGATTTCC GATTGCAAGG CGTCTTGGAC
    TCCACCTTGA ACAGCAAGAC CTACGACACC ATTAGAAACC GCCTCACCGT GGAAGAAGCC
    ACAGCGTCCG TGAGCGAGGG GGGAGGCCTC CAGGGAATCA CCATGAAGGA CAGCGACGAG
    GAGGATGAGG AGGATGATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001096182.1
    CDS238..4158
    Translation

    Target ORF information:

    RefSeq Version NM_001102712.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca2+ dependent secretion activator (cadps), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001102712.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACTATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGAGAATC AAAAGGACGC AGAAAATGTA
    GGCCGATTAG TAACGCCTGC CAAAAAGCTG GAAGATACAA TACGGCTCGC AGAACTTGTC
    ATTGAAGTTC TACAGCAGAA TGAGGAGCAC CATGCGGAGG GGAAAGAGGC GTTTGCTTGG
    TGGTCGGACT TGATGGTGGA ACACGCAGAG ACCTTCCTTT CGCTTTTCGC GGTGGATATG
    GATGCTGCTT TAGAGGTGCA ACCGCCAGAC AGCTGGGACA GCTTCCCTCT TTTTCAGCTA
    ATAAATGACT TCCTTCGAAG TGACTATAAT TTATGCAATG GGAAATTCCA CAAACACCTT
    CAAGATCTTT TTGCTCCACT CGTTGTGAGA TACGTCGACC TCATGGAGTC CTCAATTGCA
    CAGTCAATTC ACAGGGGCTT TGAGCGTGAA TCATGGGAAC CAGTAAAGAG TTTAACAAGT
    AATCTACCCA ATGTGAATCT ACCAAATGTG AATCTCCCTA AAGTACCAGT TACCTTACCA
    GTTAACCTCC CACAAATGCC TAGTTTTTCT GCACCGTCAT GGATGGCAGC TATATATGAT
    TCGGACAATG GATCAGCAAC TTCGGAAGAT CTATTCTGGA AGCTTGACGC TCTGCAAACG
    TTCATTCGAG ATCTTCATTG GCCCGAGGAG GAATTCGGGA AACACCTAGA GCAACGCCTT
    AAACTTATGG CTAGCGATAT GATCGAATCG TGTGTCAAAC GAACTAGAAT TGCATTCGAA
    GTCAAACTGC AGAAAACCAG TCGATCAACA GACTTCCGAG TACCGCAATC AATATGCACC
    ATGTTTAATG TTATGGTTGA TGCCAAAGCT CAGTCAACGA AACTTTGTAG CATGGAAATG
    GGCCAAGAGC ACCAGTACCA CTCCCAAATT GACGAGCTAA TTGAAGAAAC AGTTAAAGAG
    ATGATCACAC TCTTAGTGGC AAAGTTTGTC ACAATTCTAG AAGGAGTTCT GTCCAAACTA
    TCCAGATACG ATGAAGGGAC ATTATTTTCC TCCTTTCTGT CATTTACTAA ACCCGGGATG
    GACCTTGCCG ACGCCTACGT GACATTTGTG CGCCACTCTC AGGATGTCCT GCGTGATAAG
    GTCAATGAGG AGATATATAT AGAAAGGTTA TTTGATCAAT GGTATACCAG CTCAATGAAT
    GTTGTTTGTA CTTGGCTGAC CGACCGTATG GATCTGCAAC TTCACATTTA TCAGCTGAAA
    ACCCTAATTA GGGTCGTCAA GAAAACCTAC CGCGATTTCC GATTGCAAGG CGTCTTGGAC
    TCCACCTTGA ACAGCAAGAC CTACGACACC ATTAGAAACC GCCTCACCGT GGAAGAAGCC
    ACAGCGTCCG TGAGCGAGGG GGGAGGCCTC CAGGGAATCA CCATGAAGGA CAGCGACGAG
    GAGGATGAGG AGGATGATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30366
    Clone ID Related Accession (Same CDS sequence) XM_012960429.2
    Accession Version XM_012960429.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3990bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012960429.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTATG CTGATAGGGC ACAAAAACAT GGCATGGATG AATTTATCTC GTCCAATCCC
    TGTAACTTTG ACCACGCCAC CCTCTTTGAG ATGGTTCAGC GACTCACACT GGATCACAGA
    CTCAACGACT CGTACTCATG TCTGGGTTGG TTCAGTCCAG GTCAGGTCTT TGTACTCGAT
    GAATATTGTG CAAGATATGG AGTCCGAGGC TGCCATAGGC ATTTGTGTTA CCTTGGTGAT
    TTACTAGAAC GAGCAGAGAA TGGGTCAATG GTCGACCCAA CTTTACTTCA CTATAGTTTT
    GCCTTCTGCG CATCCCACGT ACATGGCAAC AGGCCTGATG GCATCGGAAC TGTAACCGTG
    GAAGAAAAAG AGCGCTTTGA AGAGATCAAA GAACGTCTCC GCTTGCTTTT GGAGAATCAG
    ATCACGCATT TTAGGTACTG CTTTCCATTT GGCCGACCAG AAGGTGCTTT GAAAGCCACT
    CTGTCATTGC TGGAAAGGGT TTTAATGAAA GACATAGTAA CTCCCGTCCC TCAAGAAGAA
    GTGAAAAACG TTATCCGTAA ATGCTTAGAG CAAGCAGCCT TAGTTAACTA CACGAGATTA
    TCCGAATATG CCAAAATCGA AGAGAATCAA AAGGACGCAG AAAATGTAGG CCGATTAGTA
    ACGCCTGCCA AAAAGCTCGA AGATACAATA CGGCTCGCAG AACTTGTCAT TGAAGTTCTA
    CAGCAGAATG AGGAGCACCA TGCGGAGGGG AAAGAGGCGT TTGCTTGGTG GTCGGACTTG
    ATGGTGGAAC ACGCAGAGAC CTTCCTTTCG CTTTTCGCGG TGGATATGGA TGCTGCTTTA
    GAGGTGCAAC CGCCAGACAG CTGGGACAGC TTCCCTCTTT TTCAGCTAAT AAATGACTTC
    CTTCGAAGTG ACTATAATTT ATGCAATGGG AAATTCCACA AACACCTTCA AGATCTCTTT
    GCTCCACTCG TTGTGAGATA CGTCGACCTC ATGGAGTCCT CAATTGCACA GTCAATTCAC
    AGGGGCTTTG AGCGTGAATC ATGGGAACCA GTAAAGAGTT TAACAAGTAA TCTACCCAAT
    GTGAATCTAC CAAATGTGAA TCTCCCTAAA GTACCAGTTA CCTTACCAGT TAACCTCCCA
    CAAATGCCTA GTTTTTCTGC ACCGTCATGG ATGGCAGCTA TATATGATTC GGACAATGGA
    TCAGCAACTT CAGAAGATCT ATTCTGGAAG CTTGACGCTC TGCAAACGTT CATTCGAGAT
    CTTCATTGGC CCGAGGAGGA ATTCGGGAAA CACCTAGAGC AACGCCTTAA ACTTATGGCT
    AGCGATATGA TCGAATCGTG TGTCAAACGA ACTAGAATTG CATTCGAAGT CAAACTGCAG
    AAAACCAGTC GATCAACAGA CTTCCGAGTA CCGCAATCAA TATGCACCAT GTTTAATGTT
    ATGGTTGATG CCAAAGCTCA GTCAACGAAA CTTTGTAGCA TGGAAATGGG CCAAGAGCAC
    CAGTACCACT CCCAAATTGA CGAGCTAATT GAAGAAACAG TTAAAGAGAT GATCACACTC
    TTAGTGGCAA AGTTTGTCAC AATTCTAGAA GGAGTTCTGT CCAAACTATC CAGATACGAT
    GAAGGGACAT TATTTTCCTC CTTTCTGTCA TTTACTGTGA AAGCAGCTTC CAAGTATGTG
    GATGTACCAA AACCCGGGAT GGACCTTGCC GACGCCTACG TGACATTTGT GCGCCACTCT
    CAGGATGTCC TGCGTGATAA GGTCAATGAG GAGATATATA TAGAAAGGTT ATTTGATAAA
    AGGCTGGATG GTAATAGTTC TGTCATGTAC CTGAGAATAT TGGAGCAATG GTATACCAGC
    TCAATGAATG TTGTTTGTAC TTGGCTGACC GACCGTATGG ATCTGCAACT TCACATTTAT
    CAGCTGAAAA CCCTAATTAG GGTCGTCAAG AAAACCTACC GCGATTTCCG ATTGCAAGGC
    GTCTTGGACT CCACCTTGAA CAGCAAGACC TACGACACCA TTAGAAACCG CCTCACCGTG
    GAAGAAGCCA CAGCGTCCGT GAGCGAGGGG GGAGGCCTCC AGGGAATCAC CATGAAGGAC
    AGCGACGAGG AGGATGAGGA GGATGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815883.1
    CDS744..4733
    Translation

    Target ORF information:

    RefSeq Version XM_012960429.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca++-dependent secretion activator (cadps), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012960429.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTATG CTGATAGGGC ACAAAAACAT GGCATGGATG AATTTATCTC GTCCAATCCC
    TGTAACTTTG ACCACGCCAC CCTCTTTGAG ATGGTTCAGC GACTCACACT GGATCACAGA
    CTCAACGACT CGTACTCATG TCTGGGTTGG TTCAGTCCAG GTCAGGTCTT TGTACTCGAT
    GAATATTGTG CAAGATATGG AGTCCGAGGC TGCCATAGGC ATTTGTGTTA CCTTGGTGAT
    TTACTAGAAC GAGCAGAGAA TGGGTCAATG GTCGACCCAA CTTTACTTCA CTATAGTTTT
    GCCTTCTGCG CATCCCACGT ACATGGCAAC AGGCCTGATG GCATCGGAAC TGTAACCGTG
    GAAGAAAAAG AGCGCTTTGA AGAGATCAAA GAACGTCTCC GCTTGCTTTT GGAGAATCAG
    ATCACGCATT TTAGGTACTG CTTTCCATTT GGCCGACCAG AAGGTGCTTT GAAAGCCACT
    CTGTCATTGC TGGAAAGGGT TTTAATGAAA GACATAGTAA CTCCCGTCCC TCAAGAAGAA
    GTGAAAAACG TTATCCGTAA ATGCTTAGAG CAAGCAGCCT TAGTTAACTA CACGAGATTA
    TCCGAATATG CCAAAATCGA AGAGAATCAA AAGGACGCAG AAAATGTAGG CCGATTAGTA
    ACGCCTGCCA AAAAGCTCGA AGATACAATA CGGCTCGCAG AACTTGTCAT TGAAGTTCTA
    CAGCAGAATG AGGAGCACCA TGCGGAGGGG AAAGAGGCGT TTGCTTGGTG GTCGGACTTG
    ATGGTGGAAC ACGCAGAGAC CTTCCTTTCG CTTTTCGCGG TGGATATGGA TGCTGCTTTA
    GAGGTGCAAC CGCCAGACAG CTGGGACAGC TTCCCTCTTT TTCAGCTAAT AAATGACTTC
    CTTCGAAGTG ACTATAATTT ATGCAATGGG AAATTCCACA AACACCTTCA AGATCTCTTT
    GCTCCACTCG TTGTGAGATA CGTCGACCTC ATGGAGTCCT CAATTGCACA GTCAATTCAC
    AGGGGCTTTG AGCGTGAATC ATGGGAACCA GTAAAGAGTT TAACAAGTAA TCTACCCAAT
    GTGAATCTAC CAAATGTGAA TCTCCCTAAA GTACCAGTTA CCTTACCAGT TAACCTCCCA
    CAAATGCCTA GTTTTTCTGC ACCGTCATGG ATGGCAGCTA TATATGATTC GGACAATGGA
    TCAGCAACTT CAGAAGATCT ATTCTGGAAG CTTGACGCTC TGCAAACGTT CATTCGAGAT
    CTTCATTGGC CCGAGGAGGA ATTCGGGAAA CACCTAGAGC AACGCCTTAA ACTTATGGCT
    AGCGATATGA TCGAATCGTG TGTCAAACGA ACTAGAATTG CATTCGAAGT CAAACTGCAG
    AAAACCAGTC GATCAACAGA CTTCCGAGTA CCGCAATCAA TATGCACCAT GTTTAATGTT
    ATGGTTGATG CCAAAGCTCA GTCAACGAAA CTTTGTAGCA TGGAAATGGG CCAAGAGCAC
    CAGTACCACT CCCAAATTGA CGAGCTAATT GAAGAAACAG TTAAAGAGAT GATCACACTC
    TTAGTGGCAA AGTTTGTCAC AATTCTAGAA GGAGTTCTGT CCAAACTATC CAGATACGAT
    GAAGGGACAT TATTTTCCTC CTTTCTGTCA TTTACTGTGA AAGCAGCTTC CAAGTATGTG
    GATGTACCAA AACCCGGGAT GGACCTTGCC GACGCCTACG TGACATTTGT GCGCCACTCT
    CAGGATGTCC TGCGTGATAA GGTCAATGAG GAGATATATA TAGAAAGGTT ATTTGATAAA
    AGGCTGGATG GTAATAGTTC TGTCATGTAC CTGAGAATAT TGGAGCAATG GTATACCAGC
    TCAATGAATG TTGTTTGTAC TTGGCTGACC GACCGTATGG ATCTGCAACT TCACATTTAT
    CAGCTGAAAA CCCTAATTAG GGTCGTCAAG AAAACCTACC GCGATTTCCG ATTGCAAGGC
    GTCTTGGACT CCACCTTGAA CAGCAAGACC TACGACACCA TTAGAAACCG CCTCACCGTG
    GAAGAAGCCA CAGCGTCCGT GAGCGAGGGG GGAGGCCTCC AGGGAATCAC CATGAAGGAC
    AGCGACGAGG AGGATGAGGA GGATGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30367
    Clone ID Related Accession (Same CDS sequence) XM_012960430.2
    Accession Version XM_012960430.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3984bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012960430.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTATG CTGATAGGGC ACAAAAACAT GGCATGGATG AATTTATCTC GTCCAATCCC
    TGTAACTTTG ACCACGCCAC CCTCTTTGAG ATGGTTCAGC GACTCACACT GGATCACAGA
    CTCAACGACT CGTACTCATG TCTGGGTTGG TTCAGTCCAG GTCAGGTCTT TGTACTCGAT
    GAATATTGTG CAAGATATGG AGTCCGAGGC TGCCATAGGC ATTTGTGTTA CCTTGGTGAT
    TTACTAGAAC GAGCAGAGAA TGGGTCAATG GTCGACCCAA CTTTACTTCA CTATAGTTTT
    GCCTTCTGCG CATCCCACGT ACATGGCAAC AGGCCTGATG GCATCGGAAC TGTAACCGTG
    GAAGAAAAAG AGCGCTTTGA AGAGATCAAA GAACGTCTCC GCTTGCTTTT GGAGAATCAG
    ATCACGCATT TTAGGTACTG CTTTCCATTT GGCCGACCAG AAGGTGCTTT GAAAGCCACT
    CTGTCATTGC TGGAAAGGGT TTTAATGAAA GACATAGTAA CTCCCGTCCC TCAAGAAGAA
    GTGAAAAACG TTATCCGTAA ATGCTTAGAG CAAGCAGCCT TAGTTAACTA CACGAGATTA
    TCCGAATATG CCAAAATCGA AGGGAAAAAG AGAGAAATGT ATGAACATCC TGTCTTCTGC
    TTGGCCTCCC AAGTGATGGA TTTAACCATT CAAAATGTAG GCCGATTAGT AACGCCTGCC
    AAAAAGCTCG AAGATACAAT ACGGCTCGCA GAACTTGTCA TTGAAGTTCT ACAGCAGAAT
    GAGGAGCACC ATGCGGAGGC GTTTGCTTGG TGGTCGGACT TGATGGTGGA ACACGCAGAG
    ACCTTCCTTT CGCTTTTCGC GGTGGATATG GATGCTGCTT TAGAGGTGCA ACCGCCAGAC
    AGCTGGGACA GCTTCCCTCT TTTTCAGCTA ATAAATGACT TCCTTCGAAG TGACTATAAT
    TTATGCAATG GGAAATTCCA CAAACACCTT CAAGATCTCT TTGCTCCACT CGTTGTGAGA
    TACGTCGACC TCATGGAGTC CTCAATTGCA CAGTCAATTC ACAGGGGCTT TGAGCGTGAA
    TCATGGGAAC CAGTAAAGAG TTTAACAAGT AATCTACCCA ATGTGAATCT ACCAAATGTG
    AATCTCCCTA AAGTACCAGT TACCTTACCA GTTAACCTCC CACAAATGCC TAGTTTTTCT
    GCACCGTCAT GGATGGCAGC TATATATGAT TCGGACAATG GATCAGCAAC TTCAGAAGAT
    CTATTCTGGA AGCTTGACGC TCTGCAAACG TTCATTCGAG ATCTTCATTG GCCCGAGGAG
    GAATTCGGGA AACACCTAGA GCAACGCCTT AAACTTATGG CTAGCGATAT GATCGAATCG
    TGTGTCAAAC GAACTAGAAT TGCATTCGAA GTCAAACTGC AGAAAACCAG TCGATCAACA
    GACTTCCGAG TACCGCAATC AATATGCACC ATGTTTAATG TTATGGTTGA TGCCAAAGCT
    CAGTCAACGA AACTTTGTAG CATGGAAATG GGCCAAGAGC ACCAGTACCA CTCCCAAATT
    GACGAGCTAA TTGAAGAAAC AGTTAAAGAG ATGATCACAC TCTTAGTGGC AAAGTTTGTC
    ACAATTCTAG AAGGAGTTCT GTCCAAACTA TCCAGATACG ATGAAGGGAC ATTATTTTCC
    TCCTTTCTGT CATTTACTGT GAAAGCAGCT TCCAAGTATG TGGATGTACC AAAACCCGGG
    ATGGACCTTG CCGACGCCTA CGTGACATTT GTGCGCCACT CTCAGGATGT CCTGCGTGAT
    AAGGTCAATG AGGAGATATA TATAGAAAGG TTATTTGATC AATGGTATAC CAGCTCAATG
    AATGTTGTTT GTACTTGGCT GACCGACCGT ATGGATCTGC AACTTCACAT TTATCAGCTG
    AAAACCCTAA TTAGGGTCGT CAAGAAAACC TACCGCGATT TCCGATTGCA AGGCGTCTTG
    GACTCCACCT TGAACAGCAA GACCTACGAC ACCATTAGAA ACCGCCTCAC CGTGGAAGAA
    GCCACAGCGT CCGTGAGCGA GGGGGGAGGC CTCCAGGGAA TCACCATGAA GGACAGCGAC
    GAGGAGGATG AGGAGGATGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815884.1
    CDS744..4727
    Translation

    Target ORF information:

    RefSeq Version XM_012960430.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca++-dependent secretion activator (cadps), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012960430.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTATG CTGATAGGGC ACAAAAACAT GGCATGGATG AATTTATCTC GTCCAATCCC
    TGTAACTTTG ACCACGCCAC CCTCTTTGAG ATGGTTCAGC GACTCACACT GGATCACAGA
    CTCAACGACT CGTACTCATG TCTGGGTTGG TTCAGTCCAG GTCAGGTCTT TGTACTCGAT
    GAATATTGTG CAAGATATGG AGTCCGAGGC TGCCATAGGC ATTTGTGTTA CCTTGGTGAT
    TTACTAGAAC GAGCAGAGAA TGGGTCAATG GTCGACCCAA CTTTACTTCA CTATAGTTTT
    GCCTTCTGCG CATCCCACGT ACATGGCAAC AGGCCTGATG GCATCGGAAC TGTAACCGTG
    GAAGAAAAAG AGCGCTTTGA AGAGATCAAA GAACGTCTCC GCTTGCTTTT GGAGAATCAG
    ATCACGCATT TTAGGTACTG CTTTCCATTT GGCCGACCAG AAGGTGCTTT GAAAGCCACT
    CTGTCATTGC TGGAAAGGGT TTTAATGAAA GACATAGTAA CTCCCGTCCC TCAAGAAGAA
    GTGAAAAACG TTATCCGTAA ATGCTTAGAG CAAGCAGCCT TAGTTAACTA CACGAGATTA
    TCCGAATATG CCAAAATCGA AGGGAAAAAG AGAGAAATGT ATGAACATCC TGTCTTCTGC
    TTGGCCTCCC AAGTGATGGA TTTAACCATT CAAAATGTAG GCCGATTAGT AACGCCTGCC
    AAAAAGCTCG AAGATACAAT ACGGCTCGCA GAACTTGTCA TTGAAGTTCT ACAGCAGAAT
    GAGGAGCACC ATGCGGAGGC GTTTGCTTGG TGGTCGGACT TGATGGTGGA ACACGCAGAG
    ACCTTCCTTT CGCTTTTCGC GGTGGATATG GATGCTGCTT TAGAGGTGCA ACCGCCAGAC
    AGCTGGGACA GCTTCCCTCT TTTTCAGCTA ATAAATGACT TCCTTCGAAG TGACTATAAT
    TTATGCAATG GGAAATTCCA CAAACACCTT CAAGATCTCT TTGCTCCACT CGTTGTGAGA
    TACGTCGACC TCATGGAGTC CTCAATTGCA CAGTCAATTC ACAGGGGCTT TGAGCGTGAA
    TCATGGGAAC CAGTAAAGAG TTTAACAAGT AATCTACCCA ATGTGAATCT ACCAAATGTG
    AATCTCCCTA AAGTACCAGT TACCTTACCA GTTAACCTCC CACAAATGCC TAGTTTTTCT
    GCACCGTCAT GGATGGCAGC TATATATGAT TCGGACAATG GATCAGCAAC TTCAGAAGAT
    CTATTCTGGA AGCTTGACGC TCTGCAAACG TTCATTCGAG ATCTTCATTG GCCCGAGGAG
    GAATTCGGGA AACACCTAGA GCAACGCCTT AAACTTATGG CTAGCGATAT GATCGAATCG
    TGTGTCAAAC GAACTAGAAT TGCATTCGAA GTCAAACTGC AGAAAACCAG TCGATCAACA
    GACTTCCGAG TACCGCAATC AATATGCACC ATGTTTAATG TTATGGTTGA TGCCAAAGCT
    CAGTCAACGA AACTTTGTAG CATGGAAATG GGCCAAGAGC ACCAGTACCA CTCCCAAATT
    GACGAGCTAA TTGAAGAAAC AGTTAAAGAG ATGATCACAC TCTTAGTGGC AAAGTTTGTC
    ACAATTCTAG AAGGAGTTCT GTCCAAACTA TCCAGATACG ATGAAGGGAC ATTATTTTCC
    TCCTTTCTGT CATTTACTGT GAAAGCAGCT TCCAAGTATG TGGATGTACC AAAACCCGGG
    ATGGACCTTG CCGACGCCTA CGTGACATTT GTGCGCCACT CTCAGGATGT CCTGCGTGAT
    AAGGTCAATG AGGAGATATA TATAGAAAGG TTATTTGATC AATGGTATAC CAGCTCAATG
    AATGTTGTTT GTACTTGGCT GACCGACCGT ATGGATCTGC AACTTCACAT TTATCAGCTG
    AAAACCCTAA TTAGGGTCGT CAAGAAAACC TACCGCGATT TCCGATTGCA AGGCGTCTTG
    GACTCCACCT TGAACAGCAA GACCTACGAC ACCATTAGAA ACCGCCTCAC CGTGGAAGAA
    GCCACAGCGT CCGTGAGCGA GGGGGGAGGC CTCCAGGGAA TCACCATGAA GGACAGCGAC
    GAGGAGGATG AGGAGGATGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30368
    Clone ID Related Accession (Same CDS sequence) XM_012960431.2
    Accession Version XM_012960431.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3984bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012960431.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGAAAATG TAGGCCGATT AGTAACGCCT
    GCCAAAAAGC TCGAAGATAC AATACGGCTC GCAGAACTTG TCATTGAAGT TCTACAGCAG
    AATGAGGAGC ACCATGCGGA GGGGAAAGAG GCGTTTGCTT GGTGGTCGGA CTTGATGGTG
    GAACACGCAG AGACCTTCCT TTCGCTTTTC GCGGTGGATA TGGATGCTGC TTTAGAGGTG
    CAACCGCCAG ACAGCTGGGA CAGCTTCCCT CTTTTTCAGC TAATAAATGA CTTCCTTCGA
    AGTGACTATA ATTTATGCAA TGGGAAATTC CACAAACACC TTCAAGATCT CTTTGCTCCA
    CTCGTTGTGA GATACGTCGA CCTCATGGAG TCCTCAATTG CACAGTCAAT TCACAGGGGC
    TTTGAGCGTG AATCATGGGA ACCAGTAAAG AGTTTAACAA GTAATCTACC CAATGTGAAT
    CTACCAAATG TGAATCTCCC TAAAGTACCA GTTACCTTAC CAGTTAACCT CCCACAAATG
    CCTAGTTTTT CTGCACCGTC ATGGATGGCA GCTATATATG ATTCGGACAA TGGATCAGCA
    ACTTCAGAAG ATCTATTCTG GAAGCTTGAC GCTCTGCAAA CGTTCATTCG AGATCTTCAT
    TGGCCCGAGG AGGAATTCGG GAAACACCTA GAGCAACGCC TTAAACTTAT GGCTAGCGAT
    ATGATCGAAT CGTGTGTCAA ACGAACTAGA ATTGCATTCG AAGTCAAACT GCAGAAAACC
    AGTCGATCAA CAGACTTCCG AGTACCGCAA TCAATATGCA CCATGTTTAA TGTTATGGTT
    GATGCCAAAG CTCAGTCAAC GAAACTTTGT AGCATGGAAA TGGGCCAAGA GCACCAGTAC
    CACTCCCAAA TTGACGAGCT AATTGAAGAA ACAGTTAAAG AGATGATCAC ACTCTTAGTG
    GCAAAGTTTG TCACAATTCT AGAAGGAGTT CTGTCCAAAC TATCCAGATA CGATGAAGGG
    ACATTATTTT CCTCCTTTCT GTCATTTACT GTGAAAGCAG CTTCCAAGTA TGTGGATGTA
    CCAAAACCCG GGATGGACCT TGCCGACGCC TACGTGACAT TTGTGCGCCA CTCTCAGGAT
    GTCCTGCGTG ATAAGGTCAA TGAGGAGATA TATATAGAAA GGTTATTTGA TAAAAGGCTG
    GATGGTAATA GTTCTGTCAT GTACCTGAGA ATATTGGAGC AATGGTATAC CAGCTCAATG
    AATGTTGTTT GTACTTGGCT GACCGACCGT ATGGATCTGC AACTTCACAT TTATCAGCTG
    AAAACCCTAA TTAGGGTCGT CAAGAAAACC TACCGCGATT TCCGATTGCA AGGCGTCTTG
    GACTCCACCT TGAACAGCAA GACCTACGAC ACCATTAGAA ACCGCCTCAC CGTGGAAGAA
    GCCACAGCGT CCGTGAGCGA GGGGGGAGGC CTCCAGGGAA TCACCATGAA GGACAGCGAC
    GAGGAGGATG AGGAGGATGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815885.1
    CDS745..4728
    Translation

    Target ORF information:

    RefSeq Version XM_012960431.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca++-dependent secretion activator (cadps), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012960431.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGAAAATG TAGGCCGATT AGTAACGCCT
    GCCAAAAAGC TCGAAGATAC AATACGGCTC GCAGAACTTG TCATTGAAGT TCTACAGCAG
    AATGAGGAGC ACCATGCGGA GGGGAAAGAG GCGTTTGCTT GGTGGTCGGA CTTGATGGTG
    GAACACGCAG AGACCTTCCT TTCGCTTTTC GCGGTGGATA TGGATGCTGC TTTAGAGGTG
    CAACCGCCAG ACAGCTGGGA CAGCTTCCCT CTTTTTCAGC TAATAAATGA CTTCCTTCGA
    AGTGACTATA ATTTATGCAA TGGGAAATTC CACAAACACC TTCAAGATCT CTTTGCTCCA
    CTCGTTGTGA GATACGTCGA CCTCATGGAG TCCTCAATTG CACAGTCAAT TCACAGGGGC
    TTTGAGCGTG AATCATGGGA ACCAGTAAAG AGTTTAACAA GTAATCTACC CAATGTGAAT
    CTACCAAATG TGAATCTCCC TAAAGTACCA GTTACCTTAC CAGTTAACCT CCCACAAATG
    CCTAGTTTTT CTGCACCGTC ATGGATGGCA GCTATATATG ATTCGGACAA TGGATCAGCA
    ACTTCAGAAG ATCTATTCTG GAAGCTTGAC GCTCTGCAAA CGTTCATTCG AGATCTTCAT
    TGGCCCGAGG AGGAATTCGG GAAACACCTA GAGCAACGCC TTAAACTTAT GGCTAGCGAT
    ATGATCGAAT CGTGTGTCAA ACGAACTAGA ATTGCATTCG AAGTCAAACT GCAGAAAACC
    AGTCGATCAA CAGACTTCCG AGTACCGCAA TCAATATGCA CCATGTTTAA TGTTATGGTT
    GATGCCAAAG CTCAGTCAAC GAAACTTTGT AGCATGGAAA TGGGCCAAGA GCACCAGTAC
    CACTCCCAAA TTGACGAGCT AATTGAAGAA ACAGTTAAAG AGATGATCAC ACTCTTAGTG
    GCAAAGTTTG TCACAATTCT AGAAGGAGTT CTGTCCAAAC TATCCAGATA CGATGAAGGG
    ACATTATTTT CCTCCTTTCT GTCATTTACT GTGAAAGCAG CTTCCAAGTA TGTGGATGTA
    CCAAAACCCG GGATGGACCT TGCCGACGCC TACGTGACAT TTGTGCGCCA CTCTCAGGAT
    GTCCTGCGTG ATAAGGTCAA TGAGGAGATA TATATAGAAA GGTTATTTGA TAAAAGGCTG
    GATGGTAATA GTTCTGTCAT GTACCTGAGA ATATTGGAGC AATGGTATAC CAGCTCAATG
    AATGTTGTTT GTACTTGGCT GACCGACCGT ATGGATCTGC AACTTCACAT TTATCAGCTG
    AAAACCCTAA TTAGGGTCGT CAAGAAAACC TACCGCGATT TCCGATTGCA AGGCGTCTTG
    GACTCCACCT TGAACAGCAA GACCTACGAC ACCATTAGAA ACCGCCTCAC CGTGGAAGAA
    GCCACAGCGT CCGTGAGCGA GGGGGGAGGC CTCCAGGGAA TCACCATGAA GGACAGCGAC
    GAGGAGGATG AGGAGGATGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30369
    Clone ID Related Accession (Same CDS sequence) XM_012960432.2
    Accession Version XM_012960432.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3972bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012960432.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTATG CTGATAGGGC ACAAAAACAT GGCATGGATG AATTTATCTC GTCCAATCCC
    TGTAACTTTG ACCACGCCAC CCTCTTTGAG ATGGTTCAGC GACTCACACT GGATCACAGA
    CTCAACGACT CGTACTCATG TCTGGGTTGG TTCAGTCCAG GTCAGGTCTT TGTACTCGAT
    GAATATTGTG CAAGATATGG AGTCCGAGGC TGCCATAGGC ATTTGTGTTA CCTTGGTGAT
    TTACTAGAAC GAGCAGAGAA TGGGTCAATG GTCGACCCAA CTTTACTTCA CTATAGTTTT
    GCCTTCTGCG CATCCCACGT ACATGGCAAC AGGCCTGATG GCATCGGAAC TGTAACCGTG
    GAAGAAAAAG AGCGCTTTGA AGAGATCAAA GAACGTCTCC GCTTGCTTTT GGAGAATCAG
    ATCACGCATT TTAGGTACTG CTTTCCATTT GGCCGACCAG AAGGTGCTTT GAAAGCCACT
    CTGTCATTGC TGGAAAGGGT TTTAATGAAA GACATAGTAA CTCCCGTCCC TCAAGAAGAA
    GTGAAAAACG TTATCCGTAA ATGCTTAGAG CAAGCAGCCT TAGTTAACTA CACGAGATTA
    TCCGAATATG CCAAAATCGA AGAAAATGTA GGCCGATTAG TAACGCCTGC CAAAAAGCTC
    GAAGATACAA TACGGCTCGC AGAACTTGTC ATTGAAGTTC TACAGCAGAA TGAGGAGCAC
    CATGCGGAGG GGAAAGAGGC GTTTGCTTGG TGGTCGGACT TGATGGTGGA ACACGCAGAG
    ACCTTCCTTT CGCTTTTCGC GGTGGATATG GATGCTGCTT TAGAGGTGCA ACCGCCAGAC
    AGCTGGGACA GCTTCCCTCT TTTTCAGCTA ATAAATGACT TCCTTCGAAG TGACTATAAT
    TTATGCAATG GGAAATTCCA CAAACACCTT CAAGATCTCT TTGCTCCACT CGTTGTGAGA
    TACGTCGACC TCATGGAGTC CTCAATTGCA CAGTCAATTC ACAGGGGCTT TGAGCGTGAA
    TCATGGGAAC CAGTAAAGAG TTTAACAAGT AATCTACCCA ATGTGAATCT ACCAAATGTG
    AATCTCCCTA AAGTACCAGT TACCTTACCA GTTAACCTCC CACAAATGCC TAGTTTTTCT
    GCACCGTCAT GGATGGCAGC TATATATGAT TCGGACAATG GATCAGCAAC TTCAGAAGAT
    CTATTCTGGA AGCTTGACGC TCTGCAAACG TTCATTCGAG ATCTTCATTG GCCCGAGGAG
    GAATTCGGGA AACACCTAGA GCAACGCCTT AAACTTATGG CTAGCGATAT GATCGAATCG
    TGTGTCAAAC GAACTAGAAT TGCATTCGAA GTCAAACTGC AGAAAACCAG TCGATCAACA
    GACTTCCGAG TACCGCAATC AATATGCACC ATGTTTAATG TTATGGTTGA TGCCAAAGCT
    CAGTCAACGA AACTTTGTAG CATGGAAATG GGCCAAGAGC ACCAGTACCA CTCCCAAATT
    GACGAGCTAA TTGAAGAAAC AGTTAAAGAG ATGATCACAC TCTTAGTGGC AAAGTTTGTC
    ACAATTCTAG AAGGAGTTCT GTCCAAACTA TCCAGATACG ATGAAGGGAC ATTATTTTCC
    TCCTTTCTGT CATTTACTGT GAAAGCAGCT TCCAAGTATG TGGATGTACC AAAACCCGGG
    ATGGACCTTG CCGACGCCTA CGTGACATTT GTGCGCCACT CTCAGGATGT CCTGCGTGAT
    AAGGTCAATG AGGAGATATA TATAGAAAGG TTATTTGATA AAAGGCTGGA TGGTAATAGT
    TCTGTCATGT ACCTGAGAAT ATTGGAGCAA TGGTATACCA GCTCAATGAA TGTTGTTTGT
    ACTTGGCTGA CCGACCGTAT GGATCTGCAA CTTCACATTT ATCAGCTGAA AACCCTAATT
    AGGGTCGTCA AGAAAACCTA CCGCGATTTC CGATTGCAAG GCGTCTTGGA CTCCACCTTG
    AACAGCAAGA CCTACGACAC CATTAGAAAC CGCCTCACCG TGGAAGAAGC CACAGCGTCC
    GTGAGCGAGG GGGGAGGCCT CCAGGGAATC ACCATGAAGG ACAGCGACGA GGAGGATGAG
    GAGGATGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815886.1
    CDS743..4714
    Translation

    Target ORF information:

    RefSeq Version XM_012960432.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca++-dependent secretion activator (cadps), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012960432.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTATG CTGATAGGGC ACAAAAACAT GGCATGGATG AATTTATCTC GTCCAATCCC
    TGTAACTTTG ACCACGCCAC CCTCTTTGAG ATGGTTCAGC GACTCACACT GGATCACAGA
    CTCAACGACT CGTACTCATG TCTGGGTTGG TTCAGTCCAG GTCAGGTCTT TGTACTCGAT
    GAATATTGTG CAAGATATGG AGTCCGAGGC TGCCATAGGC ATTTGTGTTA CCTTGGTGAT
    TTACTAGAAC GAGCAGAGAA TGGGTCAATG GTCGACCCAA CTTTACTTCA CTATAGTTTT
    GCCTTCTGCG CATCCCACGT ACATGGCAAC AGGCCTGATG GCATCGGAAC TGTAACCGTG
    GAAGAAAAAG AGCGCTTTGA AGAGATCAAA GAACGTCTCC GCTTGCTTTT GGAGAATCAG
    ATCACGCATT TTAGGTACTG CTTTCCATTT GGCCGACCAG AAGGTGCTTT GAAAGCCACT
    CTGTCATTGC TGGAAAGGGT TTTAATGAAA GACATAGTAA CTCCCGTCCC TCAAGAAGAA
    GTGAAAAACG TTATCCGTAA ATGCTTAGAG CAAGCAGCCT TAGTTAACTA CACGAGATTA
    TCCGAATATG CCAAAATCGA AGAAAATGTA GGCCGATTAG TAACGCCTGC CAAAAAGCTC
    GAAGATACAA TACGGCTCGC AGAACTTGTC ATTGAAGTTC TACAGCAGAA TGAGGAGCAC
    CATGCGGAGG GGAAAGAGGC GTTTGCTTGG TGGTCGGACT TGATGGTGGA ACACGCAGAG
    ACCTTCCTTT CGCTTTTCGC GGTGGATATG GATGCTGCTT TAGAGGTGCA ACCGCCAGAC
    AGCTGGGACA GCTTCCCTCT TTTTCAGCTA ATAAATGACT TCCTTCGAAG TGACTATAAT
    TTATGCAATG GGAAATTCCA CAAACACCTT CAAGATCTCT TTGCTCCACT CGTTGTGAGA
    TACGTCGACC TCATGGAGTC CTCAATTGCA CAGTCAATTC ACAGGGGCTT TGAGCGTGAA
    TCATGGGAAC CAGTAAAGAG TTTAACAAGT AATCTACCCA ATGTGAATCT ACCAAATGTG
    AATCTCCCTA AAGTACCAGT TACCTTACCA GTTAACCTCC CACAAATGCC TAGTTTTTCT
    GCACCGTCAT GGATGGCAGC TATATATGAT TCGGACAATG GATCAGCAAC TTCAGAAGAT
    CTATTCTGGA AGCTTGACGC TCTGCAAACG TTCATTCGAG ATCTTCATTG GCCCGAGGAG
    GAATTCGGGA AACACCTAGA GCAACGCCTT AAACTTATGG CTAGCGATAT GATCGAATCG
    TGTGTCAAAC GAACTAGAAT TGCATTCGAA GTCAAACTGC AGAAAACCAG TCGATCAACA
    GACTTCCGAG TACCGCAATC AATATGCACC ATGTTTAATG TTATGGTTGA TGCCAAAGCT
    CAGTCAACGA AACTTTGTAG CATGGAAATG GGCCAAGAGC ACCAGTACCA CTCCCAAATT
    GACGAGCTAA TTGAAGAAAC AGTTAAAGAG ATGATCACAC TCTTAGTGGC AAAGTTTGTC
    ACAATTCTAG AAGGAGTTCT GTCCAAACTA TCCAGATACG ATGAAGGGAC ATTATTTTCC
    TCCTTTCTGT CATTTACTGT GAAAGCAGCT TCCAAGTATG TGGATGTACC AAAACCCGGG
    ATGGACCTTG CCGACGCCTA CGTGACATTT GTGCGCCACT CTCAGGATGT CCTGCGTGAT
    AAGGTCAATG AGGAGATATA TATAGAAAGG TTATTTGATA AAAGGCTGGA TGGTAATAGT
    TCTGTCATGT ACCTGAGAAT ATTGGAGCAA TGGTATACCA GCTCAATGAA TGTTGTTTGT
    ACTTGGCTGA CCGACCGTAT GGATCTGCAA CTTCACATTT ATCAGCTGAA AACCCTAATT
    AGGGTCGTCA AGAAAACCTA CCGCGATTTC CGATTGCAAG GCGTCTTGGA CTCCACCTTG
    AACAGCAAGA CCTACGACAC CATTAGAAAC CGCCTCACCG TGGAAGAAGC CACAGCGTCC
    GTGAGCGAGG GGGGAGGCCT CCAGGGAATC ACCATGAAGG ACAGCGACGA GGAGGATGAG
    GAGGATGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30370
    Clone ID Related Accession (Same CDS sequence) XM_012960433.2
    Accession Version XM_012960433.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3906bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012960433.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGGGAAAA AGAGAGAAAT GTATGAACAT
    CCTGTCTTCT GCTTGGCCTC CCAAGTGATG GATTTAACCA TTCAAAATGT AGGCCGATTA
    GTAACGCCTG CCAAAAAGCT CGAAGATACA ATACGGCTCG CAGAACTTGT CATTGAAGTT
    CTACAGCAGA ATGAGGAGCA CCATGCGGAG GCGTTTGCTT GGTGGTCGGA CTTGATGGTG
    GAACACGCAG AGACCTTCCT TTCGCTTTTC GCGGTGGATA TGGATGCTGC TTTAGAGGTG
    CAACCGCCAG ACAGCTGGGA CAGCTTCCCT CTTTTTCAGC TAATAAATGA CTTCCTTCGA
    AGTGACTATA ATTTATGCAA TGGGAAATTC CACAAACACC TTCAAGATCT CTTTGCTCCA
    CTCGTTGTGA GATACGTCGA CCTCATGGAG TCCTCAATTG CACAGTCAAT TCACAGGGGC
    TTTGAGCGTG AATCATGGGA ACCAGTAAAC AATGGATCAG CAACTTCAGA AGATCTATTC
    TGGAAGCTTG ACGCTCTGCA AACGTTCATT CGAGATCTTC ATTGGCCCGA GGAGGAATTC
    GGGAAACACC TAGAGCAACG CCTTAAACTT ATGGCTAGCG ATATGATCGA ATCGTGTGTC
    AAACGAACTA GAATTGCATT CGAAGTCAAA CTGCAGAAAA CCAGTCGATC AACAGACTTC
    CGAGTACCGC AATCAATATG CACCATGTTT AATGTTATGG TTGATGCCAA AGCTCAGTCA
    ACGAAACTTT GTAGCATGGA AATGGGCCAA GAGCACCAGT ACCACTCCCA AATTGACGAG
    CTAATTGAAG AAACAGTTAA AGAGATGATC ACACTCTTAG TGGCAAAGTT TGTCACAATT
    CTAGAAGGAG TTCTGTCCAA ACTATCCAGA TACGATGAAG GGACATTATT TTCCTCCTTT
    CTGTCATTTA CTGTGAAAGC AGCTTCCAAG TATGTGGATG TACCAAAACC CGGGATGGAC
    CTTGCCGACG CCTACGTGAC ATTTGTGCGC CACTCTCAGG ATGTCCTGCG TGATAAGGTC
    AATGAGGAGA TATATATAGA AAGGTTATTT GATAAAAGGC TGGATGGTAA TAGTTCTGTC
    ATGTACCTGA GAATATTGGA GCAATGGTAT ACCAGCTCAA TGAATGTTGT TTGTACTTGG
    CTGACCGACC GTATGGATCT GCAACTTCAC ATTTATCAGC TGAAAACCCT AATTAGGGTC
    GTCAAGAAAA CCTACCGCGA TTTCCGATTG CAAGGCGTCT TGGACTCCAC CTTGAACAGC
    AAGACCTACG ACACCATTAG AAACCGCCTC ACCGTGGAAG AAGCCACAGC GTCCGTGAGC
    GAGGGGGGAG GCCTCCAGGG AATCACCATG AAGGACAGCG ACGAGGAGGA TGAGGAGGAT
    GATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815887.1
    CDS744..4649
    Translation

    Target ORF information:

    RefSeq Version XM_012960433.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca++-dependent secretion activator (cadps), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012960433.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGGGAAAA AGAGAGAAAT GTATGAACAT
    CCTGTCTTCT GCTTGGCCTC CCAAGTGATG GATTTAACCA TTCAAAATGT AGGCCGATTA
    GTAACGCCTG CCAAAAAGCT CGAAGATACA ATACGGCTCG CAGAACTTGT CATTGAAGTT
    CTACAGCAGA ATGAGGAGCA CCATGCGGAG GCGTTTGCTT GGTGGTCGGA CTTGATGGTG
    GAACACGCAG AGACCTTCCT TTCGCTTTTC GCGGTGGATA TGGATGCTGC TTTAGAGGTG
    CAACCGCCAG ACAGCTGGGA CAGCTTCCCT CTTTTTCAGC TAATAAATGA CTTCCTTCGA
    AGTGACTATA ATTTATGCAA TGGGAAATTC CACAAACACC TTCAAGATCT CTTTGCTCCA
    CTCGTTGTGA GATACGTCGA CCTCATGGAG TCCTCAATTG CACAGTCAAT TCACAGGGGC
    TTTGAGCGTG AATCATGGGA ACCAGTAAAC AATGGATCAG CAACTTCAGA AGATCTATTC
    TGGAAGCTTG ACGCTCTGCA AACGTTCATT CGAGATCTTC ATTGGCCCGA GGAGGAATTC
    GGGAAACACC TAGAGCAACG CCTTAAACTT ATGGCTAGCG ATATGATCGA ATCGTGTGTC
    AAACGAACTA GAATTGCATT CGAAGTCAAA CTGCAGAAAA CCAGTCGATC AACAGACTTC
    CGAGTACCGC AATCAATATG CACCATGTTT AATGTTATGG TTGATGCCAA AGCTCAGTCA
    ACGAAACTTT GTAGCATGGA AATGGGCCAA GAGCACCAGT ACCACTCCCA AATTGACGAG
    CTAATTGAAG AAACAGTTAA AGAGATGATC ACACTCTTAG TGGCAAAGTT TGTCACAATT
    CTAGAAGGAG TTCTGTCCAA ACTATCCAGA TACGATGAAG GGACATTATT TTCCTCCTTT
    CTGTCATTTA CTGTGAAAGC AGCTTCCAAG TATGTGGATG TACCAAAACC CGGGATGGAC
    CTTGCCGACG CCTACGTGAC ATTTGTGCGC CACTCTCAGG ATGTCCTGCG TGATAAGGTC
    AATGAGGAGA TATATATAGA AAGGTTATTT GATAAAAGGC TGGATGGTAA TAGTTCTGTC
    ATGTACCTGA GAATATTGGA GCAATGGTAT ACCAGCTCAA TGAATGTTGT TTGTACTTGG
    CTGACCGACC GTATGGATCT GCAACTTCAC ATTTATCAGC TGAAAACCCT AATTAGGGTC
    GTCAAGAAAA CCTACCGCGA TTTCCGATTG CAAGGCGTCT TGGACTCCAC CTTGAACAGC
    AAGACCTACG ACACCATTAG AAACCGCCTC ACCGTGGAAG AAGCCACAGC GTCCGTGAGC
    GAGGGGGGAG GCCTCCAGGG AATCACCATG AAGGACAGCG ACGAGGAGGA TGAGGAGGAT
    GATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30371
    Clone ID Related Accession (Same CDS sequence) XM_012960434.2
    Accession Version XM_012960434.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3900bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012960434.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTATG CTGATAGGGC ACAAAAACAT GGCATGGATG AATTTATCTC GTCCAATCCC
    TGTAACTTTG ACCACGCCAC CCTCTTTGAG ATGGTTCAGC GACTCACACT GGATCACAGA
    CTCAACGACT CGTACTCATG TCTGGGTTGG TTCAGTCCAG GTCAGGTCTT TGTACTCGAT
    GAATATTGTG CAAGATATGG AGTCCGAGGC TGCCATAGGC ATTTGTGTTA CCTTGGTGAT
    TTACTAGAAC GAGCAGAGAA TGGGTCAATG GTCGACCCAA CTTTACTTCA CTATAGTTTT
    GCCTTCTGCG CATCCCACGT ACATGGCAAC AGGCCTGATG GCATCGGAAC TGTAACCGTG
    GAAGAAAAAG AGCGCTTTGA AGAGATCAAA GAACGTCTCC GCTTGCTTTT GGAGAATCAG
    ATCACGCATT TTAGGTACTG CTTTCCATTT GGCCGACCAG AAGGTGCTTT GAAAGCCACT
    CTGTCATTGC TGGAAAGGGT TTTAATGAAA GACATAGTAA CTCCCGTCCC TCAAGAAGAA
    GTGAAAAACG TTATCCGTAA ATGCTTAGAG CAAGCAGCCT TAGTTAACTA CACGAGATTA
    TCCGAATATG CCAAAATCGA AGAGAATCAA AAGGACGCAG AAAATGTAGG CCGATTAGTA
    ACGCCTGCCA AAAAGCTCGA AGATACAATA CGGCTCGCAG AACTTGTCAT TGAAGTTCTA
    CAGCAGAATG AGGAGCACCA TGCGGAGGCG TTTGCTTGGT GGTCGGACTT GATGGTGGAA
    CACGCAGAGA CCTTCCTTTC GCTTTTCGCG GTGGATATGG ATGCTGCTTT AGAGGTGCAA
    CCGCCAGACA GCTGGGACAG CTTCCCTCTT TTTCAGCTAA TAAATGACTT CCTTCGAAGT
    GACTATAATT TATGCAATGG GAAATTCCAC AAACACCTTC AAGATCTCTT TGCTCCACTC
    GTTGTGAGAT ACGTCGACCT CATGGAGTCC TCAATTGCAC AGTCAATTCA CAGGGGCTTT
    GAGCGTGAAT CATGGGAACC AGTAAAGAGT TTAACAAGTA ATCTACCCAA TGTGAATCTA
    CCAAATGTGA ATCTCCCTAA AGTACCAGTT ACCTTACCAG TTAACCTCCC ACAAATGCCT
    AGTTTTTCTG CACCGTCATG GATGGCAGCT ATATATGATT CGGACAATGG ATCAGCAACT
    TCAGAAGATC TATTCTGGAA GCTTGACGCT CTGCAAACGT TCATTCGAGA TCTTCATTGG
    CCCGAGGAGG AATTCGGGAA ACACCTAGAG CAACGCCTTA AACTTATGGC TAGCGATATG
    ATCGAATCGT GTGTCAAACG AACTAGAATT GCATTCGAAG TCAAACTGCA GAAAACCAGT
    CGATCAACAG ACTTCCGAGT ACCGCAATCA ATATGCACCA TGTTTAATGT TATGGTTGAT
    GCCAAAGCTC AGTCAACGAA ACTTTGTAGC ATGGAAATGG GCCAAGAGCA CCAGTACCAC
    TCCCAAATTG ACGAGCTAAT TGAAGAAACA GTTAAAGAGA TGATCACACT CTTAGTGGCA
    AAGTTTGTCA CAATTCTAGA AGGAGTTCTG TCCAAACTAT CCAGATACGA TGAAGGGACA
    TTATTTTCCT CCTTTCTGTC ATTTACTAAA CCCGGGATGG ACCTTGCCGA CGCCTACGTG
    ACATTTGTGC GCCACTCTCA GGATGTCCTG CGTGATAAGG TCAATGAGGA GATATATATA
    GAAAGGTTAT TTGATCAATG GTATACCAGC TCAATGAATG TTGTTTGTAC TTGGCTGACC
    GACCGTATGG ATCTGCAACT TCACATTTAT CAGCTGAAAA CCCTAATTAG GGTCGTCAAG
    AAAACCTACC GCGATTTCCG ATTGCAAGGC GTCTTGGACT CCACCTTGAA CAGCAAGACC
    TACGACACCA TTAGAAACCG CCTCACCGTG GAAGAAGCCA CAGCGTCCGT GAGCGAGGGG
    GGAGGCCTCC AGGGAATCAC CATGAAGGAC AGCGACGAGG AGGATGAGGA GGATGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815888.1
    CDS743..4642
    Translation

    Target ORF information:

    RefSeq Version XM_012960434.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca++-dependent secretion activator (cadps), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012960434.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTATG CTGATAGGGC ACAAAAACAT GGCATGGATG AATTTATCTC GTCCAATCCC
    TGTAACTTTG ACCACGCCAC CCTCTTTGAG ATGGTTCAGC GACTCACACT GGATCACAGA
    CTCAACGACT CGTACTCATG TCTGGGTTGG TTCAGTCCAG GTCAGGTCTT TGTACTCGAT
    GAATATTGTG CAAGATATGG AGTCCGAGGC TGCCATAGGC ATTTGTGTTA CCTTGGTGAT
    TTACTAGAAC GAGCAGAGAA TGGGTCAATG GTCGACCCAA CTTTACTTCA CTATAGTTTT
    GCCTTCTGCG CATCCCACGT ACATGGCAAC AGGCCTGATG GCATCGGAAC TGTAACCGTG
    GAAGAAAAAG AGCGCTTTGA AGAGATCAAA GAACGTCTCC GCTTGCTTTT GGAGAATCAG
    ATCACGCATT TTAGGTACTG CTTTCCATTT GGCCGACCAG AAGGTGCTTT GAAAGCCACT
    CTGTCATTGC TGGAAAGGGT TTTAATGAAA GACATAGTAA CTCCCGTCCC TCAAGAAGAA
    GTGAAAAACG TTATCCGTAA ATGCTTAGAG CAAGCAGCCT TAGTTAACTA CACGAGATTA
    TCCGAATATG CCAAAATCGA AGAGAATCAA AAGGACGCAG AAAATGTAGG CCGATTAGTA
    ACGCCTGCCA AAAAGCTCGA AGATACAATA CGGCTCGCAG AACTTGTCAT TGAAGTTCTA
    CAGCAGAATG AGGAGCACCA TGCGGAGGCG TTTGCTTGGT GGTCGGACTT GATGGTGGAA
    CACGCAGAGA CCTTCCTTTC GCTTTTCGCG GTGGATATGG ATGCTGCTTT AGAGGTGCAA
    CCGCCAGACA GCTGGGACAG CTTCCCTCTT TTTCAGCTAA TAAATGACTT CCTTCGAAGT
    GACTATAATT TATGCAATGG GAAATTCCAC AAACACCTTC AAGATCTCTT TGCTCCACTC
    GTTGTGAGAT ACGTCGACCT CATGGAGTCC TCAATTGCAC AGTCAATTCA CAGGGGCTTT
    GAGCGTGAAT CATGGGAACC AGTAAAGAGT TTAACAAGTA ATCTACCCAA TGTGAATCTA
    CCAAATGTGA ATCTCCCTAA AGTACCAGTT ACCTTACCAG TTAACCTCCC ACAAATGCCT
    AGTTTTTCTG CACCGTCATG GATGGCAGCT ATATATGATT CGGACAATGG ATCAGCAACT
    TCAGAAGATC TATTCTGGAA GCTTGACGCT CTGCAAACGT TCATTCGAGA TCTTCATTGG
    CCCGAGGAGG AATTCGGGAA ACACCTAGAG CAACGCCTTA AACTTATGGC TAGCGATATG
    ATCGAATCGT GTGTCAAACG AACTAGAATT GCATTCGAAG TCAAACTGCA GAAAACCAGT
    CGATCAACAG ACTTCCGAGT ACCGCAATCA ATATGCACCA TGTTTAATGT TATGGTTGAT
    GCCAAAGCTC AGTCAACGAA ACTTTGTAGC ATGGAAATGG GCCAAGAGCA CCAGTACCAC
    TCCCAAATTG ACGAGCTAAT TGAAGAAACA GTTAAAGAGA TGATCACACT CTTAGTGGCA
    AAGTTTGTCA CAATTCTAGA AGGAGTTCTG TCCAAACTAT CCAGATACGA TGAAGGGACA
    TTATTTTCCT CCTTTCTGTC ATTTACTAAA CCCGGGATGG ACCTTGCCGA CGCCTACGTG
    ACATTTGTGC GCCACTCTCA GGATGTCCTG CGTGATAAGG TCAATGAGGA GATATATATA
    GAAAGGTTAT TTGATCAATG GTATACCAGC TCAATGAATG TTGTTTGTAC TTGGCTGACC
    GACCGTATGG ATCTGCAACT TCACATTTAT CAGCTGAAAA CCCTAATTAG GGTCGTCAAG
    AAAACCTACC GCGATTTCCG ATTGCAAGGC GTCTTGGACT CCACCTTGAA CAGCAAGACC
    TACGACACCA TTAGAAACCG CCTCACCGTG GAAGAAGCCA CAGCGTCCGT GAGCGAGGGG
    GGAGGCCTCC AGGGAATCAC CATGAAGGAC AGCGACGAGG AGGATGAGGA GGATGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30372
    Clone ID Related Accession (Same CDS sequence) XM_012960435.2
    Accession Version XM_012960435.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3846bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012960435.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTATG CTGATAGGGC ACAAAAACAT GGCATGGATG AATTTATCTC GTCCAATCCC
    TGTAACTTTG ACCACGCCAC CCTCTTTGAG ATGGTTCAGC GACTCACACT GGATCACAGA
    CTCAACGACT CGTACTCATG TCTGGGTTGG TTCAGTCCAG GTCAGGTCTT TGTACTCGAT
    GAATATTGTG CAAGATATGG AGTCCGAGGC TGCCATAGGC ATTTGTGTTA CCTTGGTGAT
    TTACTAGAAC GAGCAGAGAA TGGGTCAATG GTCGACCCAA CTTTACTTCA CTATAGTTTT
    GCCTTCTGCG CATCCCACGT ACATGGCAAC AGGCCTGATG GCATCGGAAC TGTAACCGTG
    GAAGAAAAAG AGCGCTTTGA AGAGATCAAA GAACGTCTCC GCTTGCTTTT GGAGAATCAG
    ATCACGCATT TTAGGTACTG CTTTCCATTT GGCCGACCAG AAGGTGCTTT GAAAGCCACT
    CTGTCATTGC TGGAAAGGGT TTTAATGAAA GACATAGTAA CTCCCGTCCC TCAAGAAGAA
    GTGAAAAACG TTATCCGTAA ATGCTTAGAG CAAGCAGCCT TAGTTAACTA CACGAGATTA
    TCCGAATATG CCAAAATCGA AGGGAAAAAG AGAGAAATGT ATGAACATCC TGTCTTCTGC
    TTGGCCTCCC AAGTGATGGA TTTAACCATT CAAAATGTAG GCCGATTAGT AACGCCTGCC
    AAAAAGCTCG AAGATACAAT ACGGCTCGCA GAACTTGTCA TTGAAGTTCT ACAGCAGAAT
    GAGGAGCACC ATGCGGAGGC GTTTGCTTGG TGGTCGGACT TGATGGTGGA ACACGCAGAG
    ACCTTCCTTT CGCTTTTCGC GGTGGATATG GATGCTGCTT TAGAGGTGCA ACCGCCAGAC
    AGCTGGGACA GCTTCCCTCT TTTTCAGCTA ATAAATGACT TCCTTCGAAG TGACTATAAT
    TTATGCAATG GGAAATTCCA CAAACACCTT CAAGATCTCT TTGCTCCACT CGTTGTGAGA
    TACGTCGACC TCATGGAGTC CTCAATTGCA CAGTCAATTC ACAGGGGCTT TGAGCGTGAA
    TCATGGGAAC CAGTAAACAA TGGATCAGCA ACTTCAGAAG ATCTATTCTG GAAGCTTGAC
    GCTCTGCAAA CGTTCATTCG AGATCTTCAT TGGCCCGAGG AGGAATTCGG GAAACACCTA
    GAGCAACGCC TTAAACTTAT GGCTAGCGAT ATGATCGAAT CGTGTGTCAA ACGAACTAGA
    ATTGCATTCG AAGTCAAACT GCAGAAAACC AGTCGATCAA CAGACTTCCG AGTACCGCAA
    TCAATATGCA CCATGTTTAA TGTTATGGTT GATGCCAAAG CTCAGTCAAC GAAACTTTGT
    AGCATGGAAA TGGGCCAAGA GCACCAGTAC CACTCCCAAA TTGACGAGCT AATTGAAGAA
    ACAGTTAAAG AGATGATCAC ACTCTTAGTG GCAAAGTTTG TCACAATTCT AGAAGGAGTT
    CTGTCCAAAC TATCCAGATA CGATGAAGGG ACATTATTTT CCTCCTTTCT GTCATTTACT
    GTGAAAGCAG CTTCCAAGTA TGTGGATGTA CCAAAACCCG GGATGGACCT TGCCGACGCC
    TACGTGACAT TTGTGCGCCA CTCTCAGGAT GTCCTGCGTG ATAAGGTCAA TGAGGAGATA
    TATATAGAAA GGTTATTTGA TCAATGGTAT ACCAGCTCAA TGAATGTTGT TTGTACTTGG
    CTGACCGACC GTATGGATCT GCAACTTCAC ATTTATCAGC TGAAAACCCT AATTAGGGTC
    GTCAAGAAAA CCTACCGCGA TTTCCGATTG CAAGGCGTCT TGGACTCCAC CTTGAACAGC
    AAGACCTACG ACACCATTAG AAACCGCCTC ACCGTGGAAG AAGCCACAGC GTCCGTGAGC
    GAGGGGGGAG GCCTCCAGGG AATCACCATG AAGGACAGCG ACGAGGAGGA TGAGGAGGAT
    GATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815889.1
    CDS742..4587
    Translation

    Target ORF information:

    RefSeq Version XM_012960435.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca++-dependent secretion activator (cadps), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012960435.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTATG CTGATAGGGC ACAAAAACAT GGCATGGATG AATTTATCTC GTCCAATCCC
    TGTAACTTTG ACCACGCCAC CCTCTTTGAG ATGGTTCAGC GACTCACACT GGATCACAGA
    CTCAACGACT CGTACTCATG TCTGGGTTGG TTCAGTCCAG GTCAGGTCTT TGTACTCGAT
    GAATATTGTG CAAGATATGG AGTCCGAGGC TGCCATAGGC ATTTGTGTTA CCTTGGTGAT
    TTACTAGAAC GAGCAGAGAA TGGGTCAATG GTCGACCCAA CTTTACTTCA CTATAGTTTT
    GCCTTCTGCG CATCCCACGT ACATGGCAAC AGGCCTGATG GCATCGGAAC TGTAACCGTG
    GAAGAAAAAG AGCGCTTTGA AGAGATCAAA GAACGTCTCC GCTTGCTTTT GGAGAATCAG
    ATCACGCATT TTAGGTACTG CTTTCCATTT GGCCGACCAG AAGGTGCTTT GAAAGCCACT
    CTGTCATTGC TGGAAAGGGT TTTAATGAAA GACATAGTAA CTCCCGTCCC TCAAGAAGAA
    GTGAAAAACG TTATCCGTAA ATGCTTAGAG CAAGCAGCCT TAGTTAACTA CACGAGATTA
    TCCGAATATG CCAAAATCGA AGGGAAAAAG AGAGAAATGT ATGAACATCC TGTCTTCTGC
    TTGGCCTCCC AAGTGATGGA TTTAACCATT CAAAATGTAG GCCGATTAGT AACGCCTGCC
    AAAAAGCTCG AAGATACAAT ACGGCTCGCA GAACTTGTCA TTGAAGTTCT ACAGCAGAAT
    GAGGAGCACC ATGCGGAGGC GTTTGCTTGG TGGTCGGACT TGATGGTGGA ACACGCAGAG
    ACCTTCCTTT CGCTTTTCGC GGTGGATATG GATGCTGCTT TAGAGGTGCA ACCGCCAGAC
    AGCTGGGACA GCTTCCCTCT TTTTCAGCTA ATAAATGACT TCCTTCGAAG TGACTATAAT
    TTATGCAATG GGAAATTCCA CAAACACCTT CAAGATCTCT TTGCTCCACT CGTTGTGAGA
    TACGTCGACC TCATGGAGTC CTCAATTGCA CAGTCAATTC ACAGGGGCTT TGAGCGTGAA
    TCATGGGAAC CAGTAAACAA TGGATCAGCA ACTTCAGAAG ATCTATTCTG GAAGCTTGAC
    GCTCTGCAAA CGTTCATTCG AGATCTTCAT TGGCCCGAGG AGGAATTCGG GAAACACCTA
    GAGCAACGCC TTAAACTTAT GGCTAGCGAT ATGATCGAAT CGTGTGTCAA ACGAACTAGA
    ATTGCATTCG AAGTCAAACT GCAGAAAACC AGTCGATCAA CAGACTTCCG AGTACCGCAA
    TCAATATGCA CCATGTTTAA TGTTATGGTT GATGCCAAAG CTCAGTCAAC GAAACTTTGT
    AGCATGGAAA TGGGCCAAGA GCACCAGTAC CACTCCCAAA TTGACGAGCT AATTGAAGAA
    ACAGTTAAAG AGATGATCAC ACTCTTAGTG GCAAAGTTTG TCACAATTCT AGAAGGAGTT
    CTGTCCAAAC TATCCAGATA CGATGAAGGG ACATTATTTT CCTCCTTTCT GTCATTTACT
    GTGAAAGCAG CTTCCAAGTA TGTGGATGTA CCAAAACCCG GGATGGACCT TGCCGACGCC
    TACGTGACAT TTGTGCGCCA CTCTCAGGAT GTCCTGCGTG ATAAGGTCAA TGAGGAGATA
    TATATAGAAA GGTTATTTGA TCAATGGTAT ACCAGCTCAA TGAATGTTGT TTGTACTTGG
    CTGACCGACC GTATGGATCT GCAACTTCAC ATTTATCAGC TGAAAACCCT AATTAGGGTC
    GTCAAGAAAA CCTACCGCGA TTTCCGATTG CAAGGCGTCT TGGACTCCAC CTTGAACAGC
    AAGACCTACG ACACCATTAG AAACCGCCTC ACCGTGGAAG AAGCCACAGC GTCCGTGAGC
    GAGGGGGGAG GCCTCCAGGG AATCACCATG AAGGACAGCG ACGAGGAGGA TGAGGAGGAT
    GATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30373
    Clone ID Related Accession (Same CDS sequence) XM_012960436.2
    Accession Version XM_012960436.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3825bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012960436.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTATG CTGATAGGGC ACAAAAACAT GGCATGGATG AATTTATCTC GTCCAATCCC
    TGTAACTTTG ACCACGCCAC CCTCTTTGAG ATGGTTCAGC GACTCACACT GGATCACAGA
    CTCAACGACT CGTACTCATG TCTGGGTTGG TTCAGTCCAG GTCAGGTCTT TGTACTCGAT
    GAATATTGTG CAAGATATGG AGTCCGAGGC TGCCATAGGC ATTTGTGTTA CCTTGGTGAT
    TTACTAGAAC GAGCAGAGAA TGGGTCAATG GTCGACCCAA CTTTACTTCA CTATAGTTTT
    GCCTTCTGCG CATCCCACGT ACATGGCAAC AGGCCTGATG GCATCGGAAC TGTAACCGTG
    GAAGAAAAAG AGCGCTTTGA AGAGATCAAA GAACGTCTCC GCTTGCTTTT GGAGAATCAG
    ATCACGCATT TTAGGTACTG CTTTCCATTT GGCCGACCAG AAGGTGCTTT GAAAGCCACT
    CTGTCATTGC TGGAAAGGGT TTTAATGAAA GACATAGTAA CTCCCGTCCC TCAAGAAGAA
    GTGAAAAACG TTATCCGTAA ATGCTTAGAG CAAGCAGCCT TAGTTAACTA CACGAGATTA
    TCCGAATATG CCAAAATCGA AGAAAATGTA GGCCGATTAG TAACGCCTGC CAAAAAGCTC
    GAAGATACAA TACGGCTCGC AGAACTTGTC ATTGAAGTTC TACAGCAGAA TGAGGAGCAC
    CATGCGGAGG CGTTTGCTTG GTGGTCGGAC TTGATGGTGG AACACGCAGA GACCTTCCTT
    TCGCTTTTCG CGGTGGATAT GGATGCTGCT TTAGAGGTGC AACCGCCAGA CAGCTGGGAC
    AGCTTCCCTC TTTTTCAGCT AATAAATGAC TTCCTTCGAA GTGACTATAA TTTATGCAAT
    GGGAAATTCC ACAAACACCT TCAAGATCTC TTTGCTCCAC TCGTTGTGAG ATACGTCGAC
    CTCATGGAGT CCTCAATTGC ACAGTCAATT CACAGGGGCT TTGAGCGTGA ATCATGGGAA
    CCAGTAAACA ATGGATCAGC AACTTCAGAA GATCTATTCT GGAAGCTTGA CGCTCTGCAA
    ACGTTCATTC GAGATCTTCA TTGGCCCGAG GAGGAATTCG GGAAACACCT AGAGCAACGC
    CTTAAACTTA TGGCTAGCGA TATGATCGAA TCGTGTGTCA AACGAACTAG AATTGCATTC
    GAAGTCAAAC TGCAGAAAAC CAGTCGATCA ACAGACTTCC GAGTACCGCA ATCAATATGC
    ACCATGTTTA ATGTTATGGT TGATGCCAAA GCTCAGTCAA CGAAACTTTG TAGCATGGAA
    ATGGGCCAAG AGCACCAGTA CCACTCCCAA ATTGACGAGC TAATTGAAGA AACAGTTAAA
    GAGATGATCA CACTCTTAGT GGCAAAGTTT GTCACAATTC TAGAAGGAGT TCTGTCCAAA
    CTATCCAGAT ACGATGAAGG GACATTATTT TCCTCCTTTC TGTCATTTAC TGTGAAAGCA
    GCTTCCAAGT ATGTGGATGT ACCAAAACCC GGGATGGACC TTGCCGACGC CTACGTGACA
    TTTGTGCGCC ACTCTCAGGA TGTCCTGCGT GATAAGGTCA ATGAGGAGAT ATATATAGAA
    AGGTTATTTG ATAAAAGGCT GGATGGTAAT AGTTCTGTCA TGTACCTGAG AATATTGGAG
    CAATGGTATA CCAGCTCAAT GAATGTTGTT TGTACTTGGC TGACCGACCG TATGGATCTG
    CAACTTCACA TTTATCAGCT GAAAACCCTA ATTAGGGTCG TCAAGAAAAC CTACCGCGAT
    TTCCGATTGC AAGGCGTCTT GGACTCCACC TTGAACAGCA AGACCTACGA CACCATTAGA
    AACCGCCTCA CCGTGGAAGA AGCCACAGCG TCCGTGAGCG AGGGGGGAGG CCTCCAGGGA
    ATCACCATGA AGGACAGCGA CGAGGAGGAT GAGGAGGATG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815890.1
    CDS740..4564
    Translation

    Target ORF information:

    RefSeq Version XM_012960436.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca++-dependent secretion activator (cadps), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012960436.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTATG CTGATAGGGC ACAAAAACAT GGCATGGATG AATTTATCTC GTCCAATCCC
    TGTAACTTTG ACCACGCCAC CCTCTTTGAG ATGGTTCAGC GACTCACACT GGATCACAGA
    CTCAACGACT CGTACTCATG TCTGGGTTGG TTCAGTCCAG GTCAGGTCTT TGTACTCGAT
    GAATATTGTG CAAGATATGG AGTCCGAGGC TGCCATAGGC ATTTGTGTTA CCTTGGTGAT
    TTACTAGAAC GAGCAGAGAA TGGGTCAATG GTCGACCCAA CTTTACTTCA CTATAGTTTT
    GCCTTCTGCG CATCCCACGT ACATGGCAAC AGGCCTGATG GCATCGGAAC TGTAACCGTG
    GAAGAAAAAG AGCGCTTTGA AGAGATCAAA GAACGTCTCC GCTTGCTTTT GGAGAATCAG
    ATCACGCATT TTAGGTACTG CTTTCCATTT GGCCGACCAG AAGGTGCTTT GAAAGCCACT
    CTGTCATTGC TGGAAAGGGT TTTAATGAAA GACATAGTAA CTCCCGTCCC TCAAGAAGAA
    GTGAAAAACG TTATCCGTAA ATGCTTAGAG CAAGCAGCCT TAGTTAACTA CACGAGATTA
    TCCGAATATG CCAAAATCGA AGAAAATGTA GGCCGATTAG TAACGCCTGC CAAAAAGCTC
    GAAGATACAA TACGGCTCGC AGAACTTGTC ATTGAAGTTC TACAGCAGAA TGAGGAGCAC
    CATGCGGAGG CGTTTGCTTG GTGGTCGGAC TTGATGGTGG AACACGCAGA GACCTTCCTT
    TCGCTTTTCG CGGTGGATAT GGATGCTGCT TTAGAGGTGC AACCGCCAGA CAGCTGGGAC
    AGCTTCCCTC TTTTTCAGCT AATAAATGAC TTCCTTCGAA GTGACTATAA TTTATGCAAT
    GGGAAATTCC ACAAACACCT TCAAGATCTC TTTGCTCCAC TCGTTGTGAG ATACGTCGAC
    CTCATGGAGT CCTCAATTGC ACAGTCAATT CACAGGGGCT TTGAGCGTGA ATCATGGGAA
    CCAGTAAACA ATGGATCAGC AACTTCAGAA GATCTATTCT GGAAGCTTGA CGCTCTGCAA
    ACGTTCATTC GAGATCTTCA TTGGCCCGAG GAGGAATTCG GGAAACACCT AGAGCAACGC
    CTTAAACTTA TGGCTAGCGA TATGATCGAA TCGTGTGTCA AACGAACTAG AATTGCATTC
    GAAGTCAAAC TGCAGAAAAC CAGTCGATCA ACAGACTTCC GAGTACCGCA ATCAATATGC
    ACCATGTTTA ATGTTATGGT TGATGCCAAA GCTCAGTCAA CGAAACTTTG TAGCATGGAA
    ATGGGCCAAG AGCACCAGTA CCACTCCCAA ATTGACGAGC TAATTGAAGA AACAGTTAAA
    GAGATGATCA CACTCTTAGT GGCAAAGTTT GTCACAATTC TAGAAGGAGT TCTGTCCAAA
    CTATCCAGAT ACGATGAAGG GACATTATTT TCCTCCTTTC TGTCATTTAC TGTGAAAGCA
    GCTTCCAAGT ATGTGGATGT ACCAAAACCC GGGATGGACC TTGCCGACGC CTACGTGACA
    TTTGTGCGCC ACTCTCAGGA TGTCCTGCGT GATAAGGTCA ATGAGGAGAT ATATATAGAA
    AGGTTATTTG ATAAAAGGCT GGATGGTAAT AGTTCTGTCA TGTACCTGAG AATATTGGAG
    CAATGGTATA CCAGCTCAAT GAATGTTGTT TGTACTTGGC TGACCGACCG TATGGATCTG
    CAACTTCACA TTTATCAGCT GAAAACCCTA ATTAGGGTCG TCAAGAAAAC CTACCGCGAT
    TTCCGATTGC AAGGCGTCTT GGACTCCACC TTGAACAGCA AGACCTACGA CACCATTAGA
    AACCGCCTCA CCGTGGAAGA AGCCACAGCG TCCGTGAGCG AGGGGGGAGG CCTCCAGGGA
    ATCACCATGA AGGACAGCGA CGAGGAGGAT GAGGAGGATG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30374
    Clone ID Related Accession (Same CDS sequence) XM_012960437.2
    Accession Version XM_012960437.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012960437.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGAAAATG TAGGCCGATT AGTAACGCCT
    GCCAAAAAGC TCGAAGATAC AATACGGCTC GCAGAACTTG TCATTGAAGT TCTACAGCAG
    AATGAGGAGC ACCATGCGGA GGCGTTTGCT TGGTGGTCGG ACTTGATGGT GGAACACGCA
    GAGACCTTCC TTTCGCTTTT CGCGGTGGAT ATGGATGCTG CTTTAGAGGT GCAACCGCCA
    GACAGCTGGG ACAGCTTCCC TCTTTTTCAG CTAATAAATG ACTTCCTTCG AAGTGACTAT
    AATTTATGCA ATGGGAAATT CCACAAACAC CTTCAAGATC TCTTTGCTCC ACTCGTTGTG
    AGATACGTCG ACCTCATGGA GTCCTCAATT GCACAGTCAA TTCACAGGGG CTTTGAGCGT
    GAATCATGGG AACCAGTAAA CAATGGATCA GCAACTTCAG AAGATCTATT CTGGAAGCTT
    GACGCTCTGC AAACGTTCAT TCGAGATCTT CATTGGCCCG AGGAGGAATT CGGGAAACAC
    CTAGAGCAAC GCCTTAAACT TATGGCTAGC GATATGATCG AATCGTGTGT CAAACGAACT
    AGAATTGCAT TCGAAGTCAA ACTGCAGAAA ACCAGTCGAT CAACAGACTT CCGAGTACCG
    CAATCAATAT GCACCATGTT TAATGTTATG GTTGATGCCA AAGCTCAGTC AACGAAACTT
    TGTAGCATGG AAATGGGCCA AGAGCACCAG TACCACTCCC AAATTGACGA GCTAATTGAA
    GAAACAGTTA AAGAGATGAT CACACTCTTA GTGGCAAAGT TTGTCACAAT TCTAGAAGGA
    GTTCTGTCCA AACTATCCAG ATACGATGAA GGGACATTAT TTTCCTCCTT TCTGTCATTT
    ACTGTGAAAG CAGCTTCCAA GTATGTGGAT GTACCAAAAC CCGGGATGGA CCTTGCCGAC
    GCCTACGTGA CATTTGTGCG CCACTCTCAG GATGTCCTGC GTGATAAGGT CAATGAGGAG
    ATATATATAG AAAGGTTATT TGATCAATGG TATACCAGCT CAATGAATGT TGTTTGTACT
    TGGCTGACCG ACCGTATGGA TCTGCAACTT CACATTTATC AGCTGAAAAC CCTAATTAGG
    GTCGTCAAGA AAACCTACCG CGATTTCCGA TTGCAAGGCG TCTTGGACTC CACCTTGAAC
    AGCAAGACCT ACGACACCAT TAGAAACCGC CTCACCGTGG AAGAAGCCAC AGCGTCCGTG
    AGCGAGGGGG GAGGCCTCCA GGGAATCACC ATGAAGGACA GCGACGAGGA GGATGAGGAG
    GATGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815891.1
    CDS741..4529
    Translation

    Target ORF information:

    RefSeq Version XM_012960437.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca++-dependent secretion activator (cadps), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012960437.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGAAAATG TAGGCCGATT AGTAACGCCT
    GCCAAAAAGC TCGAAGATAC AATACGGCTC GCAGAACTTG TCATTGAAGT TCTACAGCAG
    AATGAGGAGC ACCATGCGGA GGCGTTTGCT TGGTGGTCGG ACTTGATGGT GGAACACGCA
    GAGACCTTCC TTTCGCTTTT CGCGGTGGAT ATGGATGCTG CTTTAGAGGT GCAACCGCCA
    GACAGCTGGG ACAGCTTCCC TCTTTTTCAG CTAATAAATG ACTTCCTTCG AAGTGACTAT
    AATTTATGCA ATGGGAAATT CCACAAACAC CTTCAAGATC TCTTTGCTCC ACTCGTTGTG
    AGATACGTCG ACCTCATGGA GTCCTCAATT GCACAGTCAA TTCACAGGGG CTTTGAGCGT
    GAATCATGGG AACCAGTAAA CAATGGATCA GCAACTTCAG AAGATCTATT CTGGAAGCTT
    GACGCTCTGC AAACGTTCAT TCGAGATCTT CATTGGCCCG AGGAGGAATT CGGGAAACAC
    CTAGAGCAAC GCCTTAAACT TATGGCTAGC GATATGATCG AATCGTGTGT CAAACGAACT
    AGAATTGCAT TCGAAGTCAA ACTGCAGAAA ACCAGTCGAT CAACAGACTT CCGAGTACCG
    CAATCAATAT GCACCATGTT TAATGTTATG GTTGATGCCA AAGCTCAGTC AACGAAACTT
    TGTAGCATGG AAATGGGCCA AGAGCACCAG TACCACTCCC AAATTGACGA GCTAATTGAA
    GAAACAGTTA AAGAGATGAT CACACTCTTA GTGGCAAAGT TTGTCACAAT TCTAGAAGGA
    GTTCTGTCCA AACTATCCAG ATACGATGAA GGGACATTAT TTTCCTCCTT TCTGTCATTT
    ACTGTGAAAG CAGCTTCCAA GTATGTGGAT GTACCAAAAC CCGGGATGGA CCTTGCCGAC
    GCCTACGTGA CATTTGTGCG CCACTCTCAG GATGTCCTGC GTGATAAGGT CAATGAGGAG
    ATATATATAG AAAGGTTATT TGATCAATGG TATACCAGCT CAATGAATGT TGTTTGTACT
    TGGCTGACCG ACCGTATGGA TCTGCAACTT CACATTTATC AGCTGAAAAC CCTAATTAGG
    GTCGTCAAGA AAACCTACCG CGATTTCCGA TTGCAAGGCG TCTTGGACTC CACCTTGAAC
    AGCAAGACCT ACGACACCAT TAGAAACCGC CTCACCGTGG AAGAAGCCAC AGCGTCCGTG
    AGCGAGGGGG GAGGCCTCCA GGGAATCACC ATGAAGGACA GCGACGAGGA GGATGAGGAG
    GATGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30375
    Clone ID Related Accession (Same CDS sequence) XM_012960438.2
    Accession Version XM_012960438.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3954bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012960438.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGAGAATC AAAAGGACGC AGAAAATGTA
    GGCCGATTAG TAACGCCTGC CAAAAAGCTC GAAGATACAA TACGGCTCGC AGAACTTGTC
    ATTGAAGTTC TACAGCAGAA TGAGGAGCAC CATGCGGAGG GGAAAGAGGC GTTTGCTTGG
    TGGTCGGACT TGATGGTGGA ACACGCAGAG ACCTTCCTTT CGCTTTTCGC GGTGGATATG
    GATGCTGCTT TAGAGGTGCA ACCGCCAGAC AGCTGGGACA GCTTCCCTCT TTTTCAGCTA
    ATAAATGACT TCCTTCGAAG TGACTATAAT TTATGCAATG GGAAATTCCA CAAACACCTT
    CAAGATCTCT TTGCTCCACT CGTTGTGAGA TACGTCGACC TCATGGAGTC CTCAATTGCA
    CAGTCAATTC ACAGGGGCTT TGAGCGTGAA TCATGGGAAC CAGTAAAGAG TTTAACAAGT
    AATCTACCCA ATGTGAATCT ACCAAATGTG AATCTCCCTA AAGTACCAGT TACCTTACCA
    GTTAACCTCC CACAAATGCC TAGTTTTTCT GCACCGTCAT GGATGGCAGC TATATATGAT
    TCGGACAATG GATCAGCAAC TTCAGAAGAT CTATTCTGGA AGCTTGACGC TCTGCAAACG
    TTCATTCGAG ATCTTCATTG GCCCGAGGAG GAATTCGGGA AACACCTAGA GCAACGCCTT
    AAACTTATGG CTAGCGATAT GATCGAATCG TGTGTCAAAC GAACTAGAAT TGCATTCGAA
    GTCAAACTGC AGAAAACCAG TCGATCAACA GACTTCCGAG TACCGCAATC AATATGCACC
    ATGTTTAATG TTATGGTTGA TGCCAAAGCT CAGTCAACGA AACTTTGTAG CATGGAAATG
    GGCCAAGAGC ACCAGTACCA CTCCCAAATT GACGAGCTAA TTGAAGAAAC AGTTAAAGAG
    ATGATCACAC TCTTAGTGGC AAAGTTTGTC ACAATTCTAG AAGGAGTTCT GTCCAAACTA
    TCCAGATACG ATGAAGGGAC ATTATTTTCC TCCTTTCTGT CATTTACTGT GAAAGCAGCT
    TCCAAGTATG TGGATGTACC AAAACCCGGG ATGGACCTTG CCGACGCCTA CGTGACATTT
    GTGCGCCACT CTCAGGATGT CCTGCGTGAT AAGGTCAATG AGGAGATATA TATAGAAAGG
    TTATTTGATC AATGGTATAC CAGCTCAATG AATGTTGTTT GTACTTGGCT GACCGACCGT
    ATGGATCTGC AACTTCACAT TTATCAGCTG AAAACCCTAA TTAGGGTCGT CAAGAAAACC
    TACCGCGATT TCCGATTGCA AGGCGTCTTG GACTCCACCT TGAACAGCAA GACCTACGAC
    ACCATTAGAA ACCGCCTCAC CGTGGAAGAA GCCACAGCGT CCGTGAGCGA GGGGGGAGGC
    CTCCAGGGAA TCACCATGAA GGACAGCGAC GAGGAGGATG AGGAGGATGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815892.1
    CDS746..4699
    Translation

    Target ORF information:

    RefSeq Version XM_012960438.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca++-dependent secretion activator (cadps), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012960438.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGAGAATC AAAAGGACGC AGAAAATGTA
    GGCCGATTAG TAACGCCTGC CAAAAAGCTC GAAGATACAA TACGGCTCGC AGAACTTGTC
    ATTGAAGTTC TACAGCAGAA TGAGGAGCAC CATGCGGAGG GGAAAGAGGC GTTTGCTTGG
    TGGTCGGACT TGATGGTGGA ACACGCAGAG ACCTTCCTTT CGCTTTTCGC GGTGGATATG
    GATGCTGCTT TAGAGGTGCA ACCGCCAGAC AGCTGGGACA GCTTCCCTCT TTTTCAGCTA
    ATAAATGACT TCCTTCGAAG TGACTATAAT TTATGCAATG GGAAATTCCA CAAACACCTT
    CAAGATCTCT TTGCTCCACT CGTTGTGAGA TACGTCGACC TCATGGAGTC CTCAATTGCA
    CAGTCAATTC ACAGGGGCTT TGAGCGTGAA TCATGGGAAC CAGTAAAGAG TTTAACAAGT
    AATCTACCCA ATGTGAATCT ACCAAATGTG AATCTCCCTA AAGTACCAGT TACCTTACCA
    GTTAACCTCC CACAAATGCC TAGTTTTTCT GCACCGTCAT GGATGGCAGC TATATATGAT
    TCGGACAATG GATCAGCAAC TTCAGAAGAT CTATTCTGGA AGCTTGACGC TCTGCAAACG
    TTCATTCGAG ATCTTCATTG GCCCGAGGAG GAATTCGGGA AACACCTAGA GCAACGCCTT
    AAACTTATGG CTAGCGATAT GATCGAATCG TGTGTCAAAC GAACTAGAAT TGCATTCGAA
    GTCAAACTGC AGAAAACCAG TCGATCAACA GACTTCCGAG TACCGCAATC AATATGCACC
    ATGTTTAATG TTATGGTTGA TGCCAAAGCT CAGTCAACGA AACTTTGTAG CATGGAAATG
    GGCCAAGAGC ACCAGTACCA CTCCCAAATT GACGAGCTAA TTGAAGAAAC AGTTAAAGAG
    ATGATCACAC TCTTAGTGGC AAAGTTTGTC ACAATTCTAG AAGGAGTTCT GTCCAAACTA
    TCCAGATACG ATGAAGGGAC ATTATTTTCC TCCTTTCTGT CATTTACTGT GAAAGCAGCT
    TCCAAGTATG TGGATGTACC AAAACCCGGG ATGGACCTTG CCGACGCCTA CGTGACATTT
    GTGCGCCACT CTCAGGATGT CCTGCGTGAT AAGGTCAATG AGGAGATATA TATAGAAAGG
    TTATTTGATC AATGGTATAC CAGCTCAATG AATGTTGTTT GTACTTGGCT GACCGACCGT
    ATGGATCTGC AACTTCACAT TTATCAGCTG AAAACCCTAA TTAGGGTCGT CAAGAAAACC
    TACCGCGATT TCCGATTGCA AGGCGTCTTG GACTCCACCT TGAACAGCAA GACCTACGAC
    ACCATTAGAA ACCGCCTCAC CGTGGAAGAA GCCACAGCGT CCGTGAGCGA GGGGGGAGGC
    CTCCAGGGAA TCACCATGAA GGACAGCGAC GAGGAGGATG AGGAGGATGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30376
    Clone ID Related Accession (Same CDS sequence) XM_012960440.2
    Accession Version XM_012960440.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3942bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012960440.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTATG CTGATAGGGC ACAAAAACAT GGCATGGATG AATTTATCTC GTCCAATCCC
    TGTAACTTTG ACCACGCCAC CCTCTTTGAG ATGGTTCAGC GACTCACACT GGATCACAGA
    CTCAACGACT CGTACTCATG TCTGGGTTGG TTCAGTCCAG GTCAGGTCTT TGTACTCGAT
    GAATATTGTG CAAGATATGG AGTCCGAGGC TGCCATAGGC ATTTGTGTTA CCTTGGTGAT
    TTACTAGAAC GAGCAGAGAA TGGGTCAATG GTCGACCCAA CTTTACTTCA CTATAGTTTT
    GCCTTCTGCG CATCCCACGT ACATGGCAAC AGGCCTGATG GCATCGGAAC TGTAACCGTG
    GAAGAAAAAG AGCGCTTTGA AGAGATCAAA GAACGTCTCC GCTTGCTTTT GGAGAATCAG
    ATCACGCATT TTAGGTACTG CTTTCCATTT GGCCGACCAG AAGGTGCTTT GAAAGCCACT
    CTGTCATTGC TGGAAAGGGT TTTAATGAAA GACATAGTAA CTCCCGTCCC TCAAGAAGAA
    GTGAAAAACG TTATCCGTAA ATGCTTAGAG CAAGCAGCCT TAGTTAACTA CACGAGATTA
    TCCGAATATG CCAAAATCGA AGAGAATCAA AAGGACGCAG AAAATGTAGG CCGATTAGTA
    ACGCCTGCCA AAAAGCTCGA AGATACAATA CGGCTCGCAG AACTTGTCAT TGAAGTTCTA
    CAGCAGAATG AGGAGCACCA TGCGGAGGGG AAAGAGGCGT TTGCTTGGTG GTCGGACTTG
    ATGGTGGAAC ACGCAGAGAC CTTCCTTTCG CTTTTCGCGG TGGATATGGA TGCTGCTTTA
    GAGGTGCAAC CGCCAGACAG CTGGGACAGC TTCCCTCTTT TTCAGCTAAT AAATGACTTC
    CTTCGAAGTG ACTATAATTT ATGCAATGGG AAATTCCACA AACACCTTCA AGATCTCTTT
    GCTCCACTCG TTGTGAGATA CGTCGACCTC ATGGAGTCCT CAATTGCACA GTCAATTCAC
    AGGGGCTTTG AGCGTGAATC ATGGGAACCA GTAAAGAGTT TAACAAGTAA TCTACCCAAT
    GTGAATCTAC CAAATGTGAA TCTCCCTAAA GTACCAGTTA CCTTACCAGT TAACCTCCCA
    CAAATGCCTA GTTTTTCTGC ACCGTCATGG ATGGCAGCTA TATATGATTC GGACAATGGA
    TCAGCAACTT CAGAAGATCT ATTCTGGAAG CTTGACGCTC TGCAAACGTT CATTCGAGAT
    CTTCATTGGC CCGAGGAGGA ATTCGGGAAA CACCTAGAGC AACGCCTTAA ACTTATGGCT
    AGCGATATGA TCGAATCGTG TGTCAAACGA ACTAGAATTG CATTCGAAGT CAAACTGCAG
    AAAACCAGTC GATCAACAGA CTTCCGAGTA CCGCAATCAA TATGCACCAT GTTTAATGTT
    ATGGTTGATG CCAAAGCTCA GTCAACGAAA CTTTGTAGCA TGGAAATGGG CCAAGAGCAC
    CAGTACCACT CCCAAATTGA CGAGCTAATT GAAGAAACAG TTAAAGAGAT GATCACACTC
    TTAGTGGCAA AGTTTGTCAC AATTCTAGAA GGAGTTCTGT CCAAACTATC CAGATACGAT
    GAAGGGACAT TATTTTCCTC CTTTCTGTCA TTTACTGTGA AAGCAGCTTC CAAGTATGTG
    GATGTACCAA AACCCGGGAT GGACCTTGCC GACGCCTACG TGACATTTGT GCGCCACTCT
    CAGGATGTCC TGCGTGATAA GGTCAATGAG GAGATATATA TAGAAAGGTT ATTTGATCAA
    TGGTATACCA GCTCAATGAA TGTTGTTTGT ACTTGGCTGA CCGACCGTAT GGATCTGCAA
    CTTCACATTT ATCAGCTGAA AACCCTAATT AGGGTCGTCA AGAAAACCTA CCGCGATTTC
    CGATTGCAAG GCGTCTTGGA CTCCACCTTG AACAGCAAGA CCTACGACAC CATTAGAAAC
    CGCCTCACCG TGGAAGAAGC CACAGCGTCC GTGAGCGAGG GGGGAGGCCT CCAGGGAATC
    ACCATGAAGG ACAGCGACGA GGAGGATGAG GAGGATGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815894.1
    CDS743..4684
    Translation

    Target ORF information:

    RefSeq Version XM_012960440.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca++-dependent secretion activator (cadps), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012960440.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTATG CTGATAGGGC ACAAAAACAT GGCATGGATG AATTTATCTC GTCCAATCCC
    TGTAACTTTG ACCACGCCAC CCTCTTTGAG ATGGTTCAGC GACTCACACT GGATCACAGA
    CTCAACGACT CGTACTCATG TCTGGGTTGG TTCAGTCCAG GTCAGGTCTT TGTACTCGAT
    GAATATTGTG CAAGATATGG AGTCCGAGGC TGCCATAGGC ATTTGTGTTA CCTTGGTGAT
    TTACTAGAAC GAGCAGAGAA TGGGTCAATG GTCGACCCAA CTTTACTTCA CTATAGTTTT
    GCCTTCTGCG CATCCCACGT ACATGGCAAC AGGCCTGATG GCATCGGAAC TGTAACCGTG
    GAAGAAAAAG AGCGCTTTGA AGAGATCAAA GAACGTCTCC GCTTGCTTTT GGAGAATCAG
    ATCACGCATT TTAGGTACTG CTTTCCATTT GGCCGACCAG AAGGTGCTTT GAAAGCCACT
    CTGTCATTGC TGGAAAGGGT TTTAATGAAA GACATAGTAA CTCCCGTCCC TCAAGAAGAA
    GTGAAAAACG TTATCCGTAA ATGCTTAGAG CAAGCAGCCT TAGTTAACTA CACGAGATTA
    TCCGAATATG CCAAAATCGA AGAGAATCAA AAGGACGCAG AAAATGTAGG CCGATTAGTA
    ACGCCTGCCA AAAAGCTCGA AGATACAATA CGGCTCGCAG AACTTGTCAT TGAAGTTCTA
    CAGCAGAATG AGGAGCACCA TGCGGAGGGG AAAGAGGCGT TTGCTTGGTG GTCGGACTTG
    ATGGTGGAAC ACGCAGAGAC CTTCCTTTCG CTTTTCGCGG TGGATATGGA TGCTGCTTTA
    GAGGTGCAAC CGCCAGACAG CTGGGACAGC TTCCCTCTTT TTCAGCTAAT AAATGACTTC
    CTTCGAAGTG ACTATAATTT ATGCAATGGG AAATTCCACA AACACCTTCA AGATCTCTTT
    GCTCCACTCG TTGTGAGATA CGTCGACCTC ATGGAGTCCT CAATTGCACA GTCAATTCAC
    AGGGGCTTTG AGCGTGAATC ATGGGAACCA GTAAAGAGTT TAACAAGTAA TCTACCCAAT
    GTGAATCTAC CAAATGTGAA TCTCCCTAAA GTACCAGTTA CCTTACCAGT TAACCTCCCA
    CAAATGCCTA GTTTTTCTGC ACCGTCATGG ATGGCAGCTA TATATGATTC GGACAATGGA
    TCAGCAACTT CAGAAGATCT ATTCTGGAAG CTTGACGCTC TGCAAACGTT CATTCGAGAT
    CTTCATTGGC CCGAGGAGGA ATTCGGGAAA CACCTAGAGC AACGCCTTAA ACTTATGGCT
    AGCGATATGA TCGAATCGTG TGTCAAACGA ACTAGAATTG CATTCGAAGT CAAACTGCAG
    AAAACCAGTC GATCAACAGA CTTCCGAGTA CCGCAATCAA TATGCACCAT GTTTAATGTT
    ATGGTTGATG CCAAAGCTCA GTCAACGAAA CTTTGTAGCA TGGAAATGGG CCAAGAGCAC
    CAGTACCACT CCCAAATTGA CGAGCTAATT GAAGAAACAG TTAAAGAGAT GATCACACTC
    TTAGTGGCAA AGTTTGTCAC AATTCTAGAA GGAGTTCTGT CCAAACTATC CAGATACGAT
    GAAGGGACAT TATTTTCCTC CTTTCTGTCA TTTACTGTGA AAGCAGCTTC CAAGTATGTG
    GATGTACCAA AACCCGGGAT GGACCTTGCC GACGCCTACG TGACATTTGT GCGCCACTCT
    CAGGATGTCC TGCGTGATAA GGTCAATGAG GAGATATATA TAGAAAGGTT ATTTGATCAA
    TGGTATACCA GCTCAATGAA TGTTGTTTGT ACTTGGCTGA CCGACCGTAT GGATCTGCAA
    CTTCACATTT ATCAGCTGAA AACCCTAATT AGGGTCGTCA AGAAAACCTA CCGCGATTTC
    CGATTGCAAG GCGTCTTGGA CTCCACCTTG AACAGCAAGA CCTACGACAC CATTAGAAAC
    CGCCTCACCG TGGAAGAAGC CACAGCGTCC GTGAGCGAGG GGGGAGGCCT CCAGGGAATC
    ACCATGAAGG ACAGCGACGA GGAGGATGAG GAGGATGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30377
    Clone ID Related Accession (Same CDS sequence) XM_012960441.2
    Accession Version XM_012960441.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3924bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012960441.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTATG CTGATAGGGC ACAAAAACAT GGCATGGATG AATTTATCTC GTCCAATCCC
    TGTAACTTTG ACCACGCCAC CCTCTTTGAG ATGGTTCAGC GACTCACACT GGATCACAGA
    CTCAACGACT CGTACTCATG TCTGGGTTGG TTCAGTCCAG GTCAGGTCTT TGTACTCGAT
    GAATATTGTG CAAGATATGG AGTCCGAGGC TGCCATAGGC ATTTGTGTTA CCTTGGTGAT
    TTACTAGAAC GAGCAGAGAA TGGGTCAATG GTCGACCCAA CTTTACTTCA CTATAGTTTT
    GCCTTCTGCG CATCCCACGT ACATGGCAAC AGGCCTGATG GCATCGGAAC TGTAACCGTG
    GAAGAAAAAG AGCGCTTTGA AGAGATCAAA GAACGTCTCC GCTTGCTTTT GGAGAATCAG
    ATCACGCATT TTAGGTACTG CTTTCCATTT GGCCGACCAG AAGGTGCTTT GAAAGCCACT
    CTGTCATTGC TGGAAAGGGT TTTAATGAAA GACATAGTAA CTCCCGTCCC TCAAGAAGAA
    GTGAAAAACG TTATCCGTAA ATGCTTAGAG CAAGCAGCCT TAGTTAACTA CACGAGATTA
    TCCGAATATG CCAAAATCGA AGAAAATGTA GGCCGATTAG TAACGCCTGC CAAAAAGCTC
    GAAGATACAA TACGGCTCGC AGAACTTGTC ATTGAAGTTC TACAGCAGAA TGAGGAGCAC
    CATGCGGAGG GGAAAGAGGC GTTTGCTTGG TGGTCGGACT TGATGGTGGA ACACGCAGAG
    ACCTTCCTTT CGCTTTTCGC GGTGGATATG GATGCTGCTT TAGAGGTGCA ACCGCCAGAC
    AGCTGGGACA GCTTCCCTCT TTTTCAGCTA ATAAATGACT TCCTTCGAAG TGACTATAAT
    TTATGCAATG GGAAATTCCA CAAACACCTT CAAGATCTCT TTGCTCCACT CGTTGTGAGA
    TACGTCGACC TCATGGAGTC CTCAATTGCA CAGTCAATTC ACAGGGGCTT TGAGCGTGAA
    TCATGGGAAC CAGTAAAGAG TTTAACAAGT AATCTACCCA ATGTGAATCT ACCAAATGTG
    AATCTCCCTA AAGTACCAGT TACCTTACCA GTTAACCTCC CACAAATGCC TAGTTTTTCT
    GCACCGTCAT GGATGGCAGC TATATATGAT TCGGACAATG GATCAGCAAC TTCAGAAGAT
    CTATTCTGGA AGCTTGACGC TCTGCAAACG TTCATTCGAG ATCTTCATTG GCCCGAGGAG
    GAATTCGGGA AACACCTAGA GCAACGCCTT AAACTTATGG CTAGCGATAT GATCGAATCG
    TGTGTCAAAC GAACTAGAAT TGCATTCGAA GTCAAACTGC AGAAAACCAG TCGATCAACA
    GACTTCCGAG TACCGCAATC AATATGCACC ATGTTTAATG TTATGGTTGA TGCCAAAGCT
    CAGTCAACGA AACTTTGTAG CATGGAAATG GGCCAAGAGC ACCAGTACCA CTCCCAAATT
    GACGAGCTAA TTGAAGAAAC AGTTAAAGAG ATGATCACAC TCTTAGTGGC AAAGTTTGTC
    ACAATTCTAG AAGGAGTTCT GTCCAAACTA TCCAGATACG ATGAAGGGAC ATTATTTTCC
    TCCTTTCTGT CATTTACTGT GAAAGCAGCT TCCAAGTATG TGGATGTACC AAAACCCGGG
    ATGGACCTTG CCGACGCCTA CGTGACATTT GTGCGCCACT CTCAGGATGT CCTGCGTGAT
    AAGGTCAATG AGGAGATATA TATAGAAAGG TTATTTGATC AATGGTATAC CAGCTCAATG
    AATGTTGTTT GTACTTGGCT GACCGACCGT ATGGATCTGC AACTTCACAT TTATCAGCTG
    AAAACCCTAA TTAGGGTCGT CAAGAAAACC TACCGCGATT TCCGATTGCA AGGCGTCTTG
    GACTCCACCT TGAACAGCAA GACCTACGAC ACCATTAGAA ACCGCCTCAC CGTGGAAGAA
    GCCACAGCGT CCGTGAGCGA GGGGGGAGGC CTCCAGGGAA TCACCATGAA GGACAGCGAC
    GAGGAGGATG AGGAGGATGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815895.1
    CDS742..4665
    Translation

    Target ORF information:

    RefSeq Version XM_012960441.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca++-dependent secretion activator (cadps), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012960441.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTATG CTGATAGGGC ACAAAAACAT GGCATGGATG AATTTATCTC GTCCAATCCC
    TGTAACTTTG ACCACGCCAC CCTCTTTGAG ATGGTTCAGC GACTCACACT GGATCACAGA
    CTCAACGACT CGTACTCATG TCTGGGTTGG TTCAGTCCAG GTCAGGTCTT TGTACTCGAT
    GAATATTGTG CAAGATATGG AGTCCGAGGC TGCCATAGGC ATTTGTGTTA CCTTGGTGAT
    TTACTAGAAC GAGCAGAGAA TGGGTCAATG GTCGACCCAA CTTTACTTCA CTATAGTTTT
    GCCTTCTGCG CATCCCACGT ACATGGCAAC AGGCCTGATG GCATCGGAAC TGTAACCGTG
    GAAGAAAAAG AGCGCTTTGA AGAGATCAAA GAACGTCTCC GCTTGCTTTT GGAGAATCAG
    ATCACGCATT TTAGGTACTG CTTTCCATTT GGCCGACCAG AAGGTGCTTT GAAAGCCACT
    CTGTCATTGC TGGAAAGGGT TTTAATGAAA GACATAGTAA CTCCCGTCCC TCAAGAAGAA
    GTGAAAAACG TTATCCGTAA ATGCTTAGAG CAAGCAGCCT TAGTTAACTA CACGAGATTA
    TCCGAATATG CCAAAATCGA AGAAAATGTA GGCCGATTAG TAACGCCTGC CAAAAAGCTC
    GAAGATACAA TACGGCTCGC AGAACTTGTC ATTGAAGTTC TACAGCAGAA TGAGGAGCAC
    CATGCGGAGG GGAAAGAGGC GTTTGCTTGG TGGTCGGACT TGATGGTGGA ACACGCAGAG
    ACCTTCCTTT CGCTTTTCGC GGTGGATATG GATGCTGCTT TAGAGGTGCA ACCGCCAGAC
    AGCTGGGACA GCTTCCCTCT TTTTCAGCTA ATAAATGACT TCCTTCGAAG TGACTATAAT
    TTATGCAATG GGAAATTCCA CAAACACCTT CAAGATCTCT TTGCTCCACT CGTTGTGAGA
    TACGTCGACC TCATGGAGTC CTCAATTGCA CAGTCAATTC ACAGGGGCTT TGAGCGTGAA
    TCATGGGAAC CAGTAAAGAG TTTAACAAGT AATCTACCCA ATGTGAATCT ACCAAATGTG
    AATCTCCCTA AAGTACCAGT TACCTTACCA GTTAACCTCC CACAAATGCC TAGTTTTTCT
    GCACCGTCAT GGATGGCAGC TATATATGAT TCGGACAATG GATCAGCAAC TTCAGAAGAT
    CTATTCTGGA AGCTTGACGC TCTGCAAACG TTCATTCGAG ATCTTCATTG GCCCGAGGAG
    GAATTCGGGA AACACCTAGA GCAACGCCTT AAACTTATGG CTAGCGATAT GATCGAATCG
    TGTGTCAAAC GAACTAGAAT TGCATTCGAA GTCAAACTGC AGAAAACCAG TCGATCAACA
    GACTTCCGAG TACCGCAATC AATATGCACC ATGTTTAATG TTATGGTTGA TGCCAAAGCT
    CAGTCAACGA AACTTTGTAG CATGGAAATG GGCCAAGAGC ACCAGTACCA CTCCCAAATT
    GACGAGCTAA TTGAAGAAAC AGTTAAAGAG ATGATCACAC TCTTAGTGGC AAAGTTTGTC
    ACAATTCTAG AAGGAGTTCT GTCCAAACTA TCCAGATACG ATGAAGGGAC ATTATTTTCC
    TCCTTTCTGT CATTTACTGT GAAAGCAGCT TCCAAGTATG TGGATGTACC AAAACCCGGG
    ATGGACCTTG CCGACGCCTA CGTGACATTT GTGCGCCACT CTCAGGATGT CCTGCGTGAT
    AAGGTCAATG AGGAGATATA TATAGAAAGG TTATTTGATC AATGGTATAC CAGCTCAATG
    AATGTTGTTT GTACTTGGCT GACCGACCGT ATGGATCTGC AACTTCACAT TTATCAGCTG
    AAAACCCTAA TTAGGGTCGT CAAGAAAACC TACCGCGATT TCCGATTGCA AGGCGTCTTG
    GACTCCACCT TGAACAGCAA GACCTACGAC ACCATTAGAA ACCGCCTCAC CGTGGAAGAA
    GCCACAGCGT CCGTGAGCGA GGGGGGAGGC CTCCAGGGAA TCACCATGAA GGACAGCGAC
    GAGGAGGATG AGGAGGATGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30378
    Clone ID Related Accession (Same CDS sequence) XM_012960442.2
    Accession Version XM_012960442.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3777bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X23
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012960442.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTATG CTGATAGGGC ACAAAAACAT GGCATGGATG AATTTATCTC GTCCAATCCC
    TGTAACTTTG ACCACGCCAC CCTCTTTGAG ATGGTTCAGC GACTCACACT GGATCACAGA
    CTCAACGACT CGTACTCATG TCTGGGTTGG TTCAGTCCAG GTCAGGTCTT TGTACTCGAT
    GAATATTGTG CAAGATATGG AGTCCGAGGC TGCCATAGGC ATTTGTGTTA CCTTGGTGAT
    TTACTAGAAC GAGCAGAGAA TGGGTCAATG GTCGACCCAA CTTTACTTCA CTATAGTTTT
    GCCTTCTGCG CATCCCACGT ACATGGCAAC AGGCCTGATG GCATCGGAAC TGTAACCGTG
    GAAGAAAAAG AGCGCTTTGA AGAGATCAAA GAACGTCTCC GCTTGCTTTT GGAGAATCAG
    ATCACGCATT TTAGGTACTG CTTTCCATTT GGCCGACCAG AAGGTGCTTT GAAAGCCACT
    CTGTCATTGC TGGAAAGGGT TTTAATGAAA GACATAGTAA CTCCCGTCCC TCAAGAAGAA
    GTGAAAAACG TTATCCGTAA ATGCTTAGAG CAAGCAGCCT TAGTTAACTA CACGAGATTA
    TCCGAATATG CCAAAATCGA AGAAAATGTA GGCCGATTAG TAACGCCTGC CAAAAAGCTC
    GAAGATACAA TACGGCTCGC AGAACTTGTC ATTGAAGTTC TACAGCAGAA TGAGGAGCAC
    CATGCGGAGG CGTTTGCTTG GTGGTCGGAC TTGATGGTGG AACACGCAGA GACCTTCCTT
    TCGCTTTTCG CGGTGGATAT GGATGCTGCT TTAGAGGTGC AACCGCCAGA CAGCTGGGAC
    AGCTTCCCTC TTTTTCAGCT AATAAATGAC TTCCTTCGAA GTGACTATAA TTTATGCAAT
    GGGAAATTCC ACAAACACCT TCAAGATCTC TTTGCTCCAC TCGTTGTGAG ATACGTCGAC
    CTCATGGAGT CCTCAATTGC ACAGTCAATT CACAGGGGCT TTGAGCGTGA ATCATGGGAA
    CCAGTAAACA ATGGATCAGC AACTTCAGAA GATCTATTCT GGAAGCTTGA CGCTCTGCAA
    ACGTTCATTC GAGATCTTCA TTGGCCCGAG GAGGAATTCG GGAAACACCT AGAGCAACGC
    CTTAAACTTA TGGCTAGCGA TATGATCGAA TCGTGTGTCA AACGAACTAG AATTGCATTC
    GAAGTCAAAC TGCAGAAAAC CAGTCGATCA ACAGACTTCC GAGTACCGCA ATCAATATGC
    ACCATGTTTA ATGTTATGGT TGATGCCAAA GCTCAGTCAA CGAAACTTTG TAGCATGGAA
    ATGGGCCAAG AGCACCAGTA CCACTCCCAA ATTGACGAGC TAATTGAAGA AACAGTTAAA
    GAGATGATCA CACTCTTAGT GGCAAAGTTT GTCACAATTC TAGAAGGAGT TCTGTCCAAA
    CTATCCAGAT ACGATGAAGG GACATTATTT TCCTCCTTTC TGTCATTTAC TGTGAAAGCA
    GCTTCCAAGT ATGTGGATGT ACCAAAACCC GGGATGGACC TTGCCGACGC CTACGTGACA
    TTTGTGCGCC ACTCTCAGGA TGTCCTGCGT GATAAGGTCA ATGAGGAGAT ATATATAGAA
    AGGTTATTTG ATCAATGGTA TACCAGCTCA ATGAATGTTG TTTGTACTTG GCTGACCGAC
    CGTATGGATC TGCAACTTCA CATTTATCAG CTGAAAACCC TAATTAGGGT CGTCAAGAAA
    ACCTACCGCG ATTTCCGATT GCAAGGCGTC TTGGACTCCA CCTTGAACAG CAAGACCTAC
    GACACCATTA GAAACCGCCT CACCGTGGAA GAAGCCACAG CGTCCGTGAG CGAGGGGGGA
    GGCCTCCAGG GAATCACCAT GAAGGACAGC GACGAGGAGG ATGAGGAGGA TGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815896.1
    CDS739..4515
    Translation

    Target ORF information:

    RefSeq Version XM_012960442.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca++-dependent secretion activator (cadps), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012960442.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTATG CTGATAGGGC ACAAAAACAT GGCATGGATG AATTTATCTC GTCCAATCCC
    TGTAACTTTG ACCACGCCAC CCTCTTTGAG ATGGTTCAGC GACTCACACT GGATCACAGA
    CTCAACGACT CGTACTCATG TCTGGGTTGG TTCAGTCCAG GTCAGGTCTT TGTACTCGAT
    GAATATTGTG CAAGATATGG AGTCCGAGGC TGCCATAGGC ATTTGTGTTA CCTTGGTGAT
    TTACTAGAAC GAGCAGAGAA TGGGTCAATG GTCGACCCAA CTTTACTTCA CTATAGTTTT
    GCCTTCTGCG CATCCCACGT ACATGGCAAC AGGCCTGATG GCATCGGAAC TGTAACCGTG
    GAAGAAAAAG AGCGCTTTGA AGAGATCAAA GAACGTCTCC GCTTGCTTTT GGAGAATCAG
    ATCACGCATT TTAGGTACTG CTTTCCATTT GGCCGACCAG AAGGTGCTTT GAAAGCCACT
    CTGTCATTGC TGGAAAGGGT TTTAATGAAA GACATAGTAA CTCCCGTCCC TCAAGAAGAA
    GTGAAAAACG TTATCCGTAA ATGCTTAGAG CAAGCAGCCT TAGTTAACTA CACGAGATTA
    TCCGAATATG CCAAAATCGA AGAAAATGTA GGCCGATTAG TAACGCCTGC CAAAAAGCTC
    GAAGATACAA TACGGCTCGC AGAACTTGTC ATTGAAGTTC TACAGCAGAA TGAGGAGCAC
    CATGCGGAGG CGTTTGCTTG GTGGTCGGAC TTGATGGTGG AACACGCAGA GACCTTCCTT
    TCGCTTTTCG CGGTGGATAT GGATGCTGCT TTAGAGGTGC AACCGCCAGA CAGCTGGGAC
    AGCTTCCCTC TTTTTCAGCT AATAAATGAC TTCCTTCGAA GTGACTATAA TTTATGCAAT
    GGGAAATTCC ACAAACACCT TCAAGATCTC TTTGCTCCAC TCGTTGTGAG ATACGTCGAC
    CTCATGGAGT CCTCAATTGC ACAGTCAATT CACAGGGGCT TTGAGCGTGA ATCATGGGAA
    CCAGTAAACA ATGGATCAGC AACTTCAGAA GATCTATTCT GGAAGCTTGA CGCTCTGCAA
    ACGTTCATTC GAGATCTTCA TTGGCCCGAG GAGGAATTCG GGAAACACCT AGAGCAACGC
    CTTAAACTTA TGGCTAGCGA TATGATCGAA TCGTGTGTCA AACGAACTAG AATTGCATTC
    GAAGTCAAAC TGCAGAAAAC CAGTCGATCA ACAGACTTCC GAGTACCGCA ATCAATATGC
    ACCATGTTTA ATGTTATGGT TGATGCCAAA GCTCAGTCAA CGAAACTTTG TAGCATGGAA
    ATGGGCCAAG AGCACCAGTA CCACTCCCAA ATTGACGAGC TAATTGAAGA AACAGTTAAA
    GAGATGATCA CACTCTTAGT GGCAAAGTTT GTCACAATTC TAGAAGGAGT TCTGTCCAAA
    CTATCCAGAT ACGATGAAGG GACATTATTT TCCTCCTTTC TGTCATTTAC TGTGAAAGCA
    GCTTCCAAGT ATGTGGATGT ACCAAAACCC GGGATGGACC TTGCCGACGC CTACGTGACA
    TTTGTGCGCC ACTCTCAGGA TGTCCTGCGT GATAAGGTCA ATGAGGAGAT ATATATAGAA
    AGGTTATTTG ATCAATGGTA TACCAGCTCA ATGAATGTTG TTTGTACTTG GCTGACCGAC
    CGTATGGATC TGCAACTTCA CATTTATCAG CTGAAAACCC TAATTAGGGT CGTCAAGAAA
    ACCTACCGCG ATTTCCGATT GCAAGGCGTC TTGGACTCCA CCTTGAACAG CAAGACCTAC
    GACACCATTA GAAACCGCCT CACCGTGGAA GAAGCCACAG CGTCCGTGAG CGAGGGGGGA
    GGCCTCCAGG GAATCACCAT GAAGGACAGC GACGAGGAGG ATGAGGAGGA TGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30362
    Clone ID Related Accession (Same CDS sequence) XM_012960425.2
    Accession Version XM_012960425.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4071bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012960425.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGGGAAAA AGAGAGAAAT GTATGAACAT
    CCTGTCTTCT GCTTGGCCTC CCAAGTGATG GATTTAACCA TTCAGAATCA AAAGGACGCA
    GAAAATGTAG GCCGATTAGT AACGCCTGCC AAAAAGCTCG AAGATACAAT ACGGCTCGCA
    GAACTTGTCA TTGAAGTTCT ACAGCAGAAT GAGGAGCACC ATGCGGAGGG GAAAGAGGCG
    TTTGCTTGGT GGTCGGACTT GATGGTGGAA CACGCAGAGA CCTTCCTTTC GCTTTTCGCG
    GTGGATATGG ATGCTGCTTT AGAGGTGCAA CCGCCAGACA GCTGGGACAG CTTCCCTCTT
    TTTCAGCTAA TAAATGACTT CCTTCGAAGT GACTATAATT TATGCAATGG GAAATTCCAC
    AAACACCTTC AAGATCTCTT TGCTCCACTC GTTGTGAGAT ACGTCGACCT CATGGAGTCC
    TCAATTGCAC AGTCAATTCA CAGGGGCTTT GAGCGTGAAT CATGGGAACC AGTAAAGAGT
    TTAACAAGTA ATCTACCCAA TGTGAATCTA CCAAATGTGA ATCTCCCTAA AGTACCAGTT
    ACCTTACCAG TTAACCTCCC ACAAATGCCT AGTTTTTCTG CACCGTCATG GATGGCAGCT
    ATATATGATT CGGACAATGG ATCAGCAACT TCAGAAGATC TATTCTGGAA GCTTGACGCT
    CTGCAAACGT TCATTCGAGA TCTTCATTGG CCCGAGGAGG AATTCGGGAA ACACCTAGAG
    CAACGCCTTA AACTTATGGC TAGCGATATG ATCGAATCGT GTGTCAAACG AACTAGAATT
    GCATTCGAAG TCAAACTGCA GAAAACCAGT CGATCAACAG ACTTCCGAGT ACCGCAATCA
    ATATGCACCA TGTTTAATGT TATGGTTGAT GCCAAAGCTC AGTCAACGAA ACTTTGTAGC
    ATGGAAATGG GCCAAGAGCA CCAGTACCAC TCCCAAATTG ACGAGCTAAT TGAAGAAACA
    GTTAAAGAGA TGATCACACT CTTAGTGGCA AAGTTTGTCA CAATTCTAGA AGGAGTTCTG
    TCCAAACTAT CCAGATACGA TGAAGGGACA TTATTTTCCT CCTTTCTGTC ATTTACTGTG
    AAAGCAGCTT CCAAGTATGT GGATGTACCA AAACCCGGGA TGGACCTTGC CGACGCCTAC
    GTGACATTTG TGCGCCACTC TCAGGATGTC CTGCGTGATA AGGTCAATGA GGAGATATAT
    ATAGAAAGGT TATTTGATAA AAGGCTGGAT GGTAATAGTT CTGTCATGTA CCTGAGAATA
    TTGGAGCAAT GGTATACCAG CTCAATGAAT GTTGTTTGTA CTTGGCTGAC CGACCGTATG
    GATCTGCAAC TTCACATTTA TCAGCTGAAA ACCCTAATTA GGGTCGTCAA GAAAACCTAC
    CGCGATTTCC GATTGCAAGG CGTCTTGGAC TCCACCTTGA ACAGCAAGAC CTACGACACC
    ATTAGAAACC GCCTCACCGT GGAAGAAGCC ACAGCGTCCG TGAGCGAGGG GGGAGGCCTC
    CAGGGAATCA CCATGAAGGA CAGCGACGAG GAGGATGAGG AGGATGATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815879.1
    CDS749..4819
    Translation

    Target ORF information:

    RefSeq Version XM_012960425.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca++-dependent secretion activator (cadps), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012960425.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGGGAAAA AGAGAGAAAT GTATGAACAT
    CCTGTCTTCT GCTTGGCCTC CCAAGTGATG GATTTAACCA TTCAGAATCA AAAGGACGCA
    GAAAATGTAG GCCGATTAGT AACGCCTGCC AAAAAGCTCG AAGATACAAT ACGGCTCGCA
    GAACTTGTCA TTGAAGTTCT ACAGCAGAAT GAGGAGCACC ATGCGGAGGG GAAAGAGGCG
    TTTGCTTGGT GGTCGGACTT GATGGTGGAA CACGCAGAGA CCTTCCTTTC GCTTTTCGCG
    GTGGATATGG ATGCTGCTTT AGAGGTGCAA CCGCCAGACA GCTGGGACAG CTTCCCTCTT
    TTTCAGCTAA TAAATGACTT CCTTCGAAGT GACTATAATT TATGCAATGG GAAATTCCAC
    AAACACCTTC AAGATCTCTT TGCTCCACTC GTTGTGAGAT ACGTCGACCT CATGGAGTCC
    TCAATTGCAC AGTCAATTCA CAGGGGCTTT GAGCGTGAAT CATGGGAACC AGTAAAGAGT
    TTAACAAGTA ATCTACCCAA TGTGAATCTA CCAAATGTGA ATCTCCCTAA AGTACCAGTT
    ACCTTACCAG TTAACCTCCC ACAAATGCCT AGTTTTTCTG CACCGTCATG GATGGCAGCT
    ATATATGATT CGGACAATGG ATCAGCAACT TCAGAAGATC TATTCTGGAA GCTTGACGCT
    CTGCAAACGT TCATTCGAGA TCTTCATTGG CCCGAGGAGG AATTCGGGAA ACACCTAGAG
    CAACGCCTTA AACTTATGGC TAGCGATATG ATCGAATCGT GTGTCAAACG AACTAGAATT
    GCATTCGAAG TCAAACTGCA GAAAACCAGT CGATCAACAG ACTTCCGAGT ACCGCAATCA
    ATATGCACCA TGTTTAATGT TATGGTTGAT GCCAAAGCTC AGTCAACGAA ACTTTGTAGC
    ATGGAAATGG GCCAAGAGCA CCAGTACCAC TCCCAAATTG ACGAGCTAAT TGAAGAAACA
    GTTAAAGAGA TGATCACACT CTTAGTGGCA AAGTTTGTCA CAATTCTAGA AGGAGTTCTG
    TCCAAACTAT CCAGATACGA TGAAGGGACA TTATTTTCCT CCTTTCTGTC ATTTACTGTG
    AAAGCAGCTT CCAAGTATGT GGATGTACCA AAACCCGGGA TGGACCTTGC CGACGCCTAC
    GTGACATTTG TGCGCCACTC TCAGGATGTC CTGCGTGATA AGGTCAATGA GGAGATATAT
    ATAGAAAGGT TATTTGATAA AAGGCTGGAT GGTAATAGTT CTGTCATGTA CCTGAGAATA
    TTGGAGCAAT GGTATACCAG CTCAATGAAT GTTGTTTGTA CTTGGCTGAC CGACCGTATG
    GATCTGCAAC TTCACATTTA TCAGCTGAAA ACCCTAATTA GGGTCGTCAA GAAAACCTAC
    CGCGATTTCC GATTGCAAGG CGTCTTGGAC TCCACCTTGA ACAGCAAGAC CTACGACACC
    ATTAGAAACC GCCTCACCGT GGAAGAAGCC ACAGCGTCCG TGAGCGAGGG GGGAGGCCTC
    CAGGGAATCA CCATGAAGGA CAGCGACGAG GAGGATGAGG AGGATGATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30363
    Clone ID Related Accession (Same CDS sequence) XM_012960426.2
    Accession Version XM_012960426.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4053bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012960426.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGGGAAAA AGAGAGAAAT GTATGAACAT
    CCTGTCTTCT GCTTGGCCTC CCAAGTGATG GATTTAACCA TTCAAAATGT AGGCCGATTA
    GTAACGCCTG CCAAAAAGCT CGAAGATACA ATACGGCTCG CAGAACTTGT CATTGAAGTT
    CTACAGCAGA ATGAGGAGCA CCATGCGGAG GGGAAAGAGG CGTTTGCTTG GTGGTCGGAC
    TTGATGGTGG AACACGCAGA GACCTTCCTT TCGCTTTTCG CGGTGGATAT GGATGCTGCT
    TTAGAGGTGC AACCGCCAGA CAGCTGGGAC AGCTTCCCTC TTTTTCAGCT AATAAATGAC
    TTCCTTCGAA GTGACTATAA TTTATGCAAT GGGAAATTCC ACAAACACCT TCAAGATCTC
    TTTGCTCCAC TCGTTGTGAG ATACGTCGAC CTCATGGAGT CCTCAATTGC ACAGTCAATT
    CACAGGGGCT TTGAGCGTGA ATCATGGGAA CCAGTAAAGA GTTTAACAAG TAATCTACCC
    AATGTGAATC TACCAAATGT GAATCTCCCT AAAGTACCAG TTACCTTACC AGTTAACCTC
    CCACAAATGC CTAGTTTTTC TGCACCGTCA TGGATGGCAG CTATATATGA TTCGGACAAT
    GGATCAGCAA CTTCAGAAGA TCTATTCTGG AAGCTTGACG CTCTGCAAAC GTTCATTCGA
    GATCTTCATT GGCCCGAGGA GGAATTCGGG AAACACCTAG AGCAACGCCT TAAACTTATG
    GCTAGCGATA TGATCGAATC GTGTGTCAAA CGAACTAGAA TTGCATTCGA AGTCAAACTG
    CAGAAAACCA GTCGATCAAC AGACTTCCGA GTACCGCAAT CAATATGCAC CATGTTTAAT
    GTTATGGTTG ATGCCAAAGC TCAGTCAACG AAACTTTGTA GCATGGAAAT GGGCCAAGAG
    CACCAGTACC ACTCCCAAAT TGACGAGCTA ATTGAAGAAA CAGTTAAAGA GATGATCACA
    CTCTTAGTGG CAAAGTTTGT CACAATTCTA GAAGGAGTTC TGTCCAAACT ATCCAGATAC
    GATGAAGGGA CATTATTTTC CTCCTTTCTG TCATTTACTG TGAAAGCAGC TTCCAAGTAT
    GTGGATGTAC CAAAACCCGG GATGGACCTT GCCGACGCCT ACGTGACATT TGTGCGCCAC
    TCTCAGGATG TCCTGCGTGA TAAGGTCAAT GAGGAGATAT ATATAGAAAG GTTATTTGAT
    AAAAGGCTGG ATGGTAATAG TTCTGTCATG TACCTGAGAA TATTGGAGCA ATGGTATACC
    AGCTCAATGA ATGTTGTTTG TACTTGGCTG ACCGACCGTA TGGATCTGCA ACTTCACATT
    TATCAGCTGA AAACCCTAAT TAGGGTCGTC AAGAAAACCT ACCGCGATTT CCGATTGCAA
    GGCGTCTTGG ACTCCACCTT GAACAGCAAG ACCTACGACA CCATTAGAAA CCGCCTCACC
    GTGGAAGAAG CCACAGCGTC CGTGAGCGAG GGGGGAGGCC TCCAGGGAAT CACCATGAAG
    GACAGCGACG AGGAGGATGA GGAGGATGAT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815880.1
    CDS749..4801
    Translation

    Target ORF information:

    RefSeq Version XM_012960426.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca++-dependent secretion activator (cadps), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012960426.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGGGAAAA AGAGAGAAAT GTATGAACAT
    CCTGTCTTCT GCTTGGCCTC CCAAGTGATG GATTTAACCA TTCAAAATGT AGGCCGATTA
    GTAACGCCTG CCAAAAAGCT CGAAGATACA ATACGGCTCG CAGAACTTGT CATTGAAGTT
    CTACAGCAGA ATGAGGAGCA CCATGCGGAG GGGAAAGAGG CGTTTGCTTG GTGGTCGGAC
    TTGATGGTGG AACACGCAGA GACCTTCCTT TCGCTTTTCG CGGTGGATAT GGATGCTGCT
    TTAGAGGTGC AACCGCCAGA CAGCTGGGAC AGCTTCCCTC TTTTTCAGCT AATAAATGAC
    TTCCTTCGAA GTGACTATAA TTTATGCAAT GGGAAATTCC ACAAACACCT TCAAGATCTC
    TTTGCTCCAC TCGTTGTGAG ATACGTCGAC CTCATGGAGT CCTCAATTGC ACAGTCAATT
    CACAGGGGCT TTGAGCGTGA ATCATGGGAA CCAGTAAAGA GTTTAACAAG TAATCTACCC
    AATGTGAATC TACCAAATGT GAATCTCCCT AAAGTACCAG TTACCTTACC AGTTAACCTC
    CCACAAATGC CTAGTTTTTC TGCACCGTCA TGGATGGCAG CTATATATGA TTCGGACAAT
    GGATCAGCAA CTTCAGAAGA TCTATTCTGG AAGCTTGACG CTCTGCAAAC GTTCATTCGA
    GATCTTCATT GGCCCGAGGA GGAATTCGGG AAACACCTAG AGCAACGCCT TAAACTTATG
    GCTAGCGATA TGATCGAATC GTGTGTCAAA CGAACTAGAA TTGCATTCGA AGTCAAACTG
    CAGAAAACCA GTCGATCAAC AGACTTCCGA GTACCGCAAT CAATATGCAC CATGTTTAAT
    GTTATGGTTG ATGCCAAAGC TCAGTCAACG AAACTTTGTA GCATGGAAAT GGGCCAAGAG
    CACCAGTACC ACTCCCAAAT TGACGAGCTA ATTGAAGAAA CAGTTAAAGA GATGATCACA
    CTCTTAGTGG CAAAGTTTGT CACAATTCTA GAAGGAGTTC TGTCCAAACT ATCCAGATAC
    GATGAAGGGA CATTATTTTC CTCCTTTCTG TCATTTACTG TGAAAGCAGC TTCCAAGTAT
    GTGGATGTAC CAAAACCCGG GATGGACCTT GCCGACGCCT ACGTGACATT TGTGCGCCAC
    TCTCAGGATG TCCTGCGTGA TAAGGTCAAT GAGGAGATAT ATATAGAAAG GTTATTTGAT
    AAAAGGCTGG ATGGTAATAG TTCTGTCATG TACCTGAGAA TATTGGAGCA ATGGTATACC
    AGCTCAATGA ATGTTGTTTG TACTTGGCTG ACCGACCGTA TGGATCTGCA ACTTCACATT
    TATCAGCTGA AAACCCTAAT TAGGGTCGTC AAGAAAACCT ACCGCGATTT CCGATTGCAA
    GGCGTCTTGG ACTCCACCTT GAACAGCAAG ACCTACGACA CCATTAGAAA CCGCCTCACC
    GTGGAAGAAG CCACAGCGTC CGTGAGCGAG GGGGGAGGCC TCCAGGGAAT CACCATGAAG
    GACAGCGACG AGGAGGATGA GGAGGATGAT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30365
    Clone ID Related Accession (Same CDS sequence) XM_012960428.2
    Accession Version XM_012960428.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4002bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-dependent secretion activator 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012960428.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGAGAATC AAAAGGACGC AGAAAATGTA
    GGCCGATTAG TAACGCCTGC CAAAAAGCTC GAAGATACAA TACGGCTCGC AGAACTTGTC
    ATTGAAGTTC TACAGCAGAA TGAGGAGCAC CATGCGGAGG GGAAAGAGGC GTTTGCTTGG
    TGGTCGGACT TGATGGTGGA ACACGCAGAG ACCTTCCTTT CGCTTTTCGC GGTGGATATG
    GATGCTGCTT TAGAGGTGCA ACCGCCAGAC AGCTGGGACA GCTTCCCTCT TTTTCAGCTA
    ATAAATGACT TCCTTCGAAG TGACTATAAT TTATGCAATG GGAAATTCCA CAAACACCTT
    CAAGATCTCT TTGCTCCACT CGTTGTGAGA TACGTCGACC TCATGGAGTC CTCAATTGCA
    CAGTCAATTC ACAGGGGCTT TGAGCGTGAA TCATGGGAAC CAGTAAAGAG TTTAACAAGT
    AATCTACCCA ATGTGAATCT ACCAAATGTG AATCTCCCTA AAGTACCAGT TACCTTACCA
    GTTAACCTCC CACAAATGCC TAGTTTTTCT GCACCGTCAT GGATGGCAGC TATATATGAT
    TCGGACAATG GATCAGCAAC TTCAGAAGAT CTATTCTGGA AGCTTGACGC TCTGCAAACG
    TTCATTCGAG ATCTTCATTG GCCCGAGGAG GAATTCGGGA AACACCTAGA GCAACGCCTT
    AAACTTATGG CTAGCGATAT GATCGAATCG TGTGTCAAAC GAACTAGAAT TGCATTCGAA
    GTCAAACTGC AGAAAACCAG TCGATCAACA GACTTCCGAG TACCGCAATC AATATGCACC
    ATGTTTAATG TTATGGTTGA TGCCAAAGCT CAGTCAACGA AACTTTGTAG CATGGAAATG
    GGCCAAGAGC ACCAGTACCA CTCCCAAATT GACGAGCTAA TTGAAGAAAC AGTTAAAGAG
    ATGATCACAC TCTTAGTGGC AAAGTTTGTC ACAATTCTAG AAGGAGTTCT GTCCAAACTA
    TCCAGATACG ATGAAGGGAC ATTATTTTCC TCCTTTCTGT CATTTACTGT GAAAGCAGCT
    TCCAAGTATG TGGATGTACC AAAACCCGGG ATGGACCTTG CCGACGCCTA CGTGACATTT
    GTGCGCCACT CTCAGGATGT CCTGCGTGAT AAGGTCAATG AGGAGATATA TATAGAAAGG
    TTATTTGATA AAAGGCTGGA TGGTAATAGT TCTGTCATGT ACCTGAGAAT ATTGGAGCAA
    TGGTATACCA GCTCAATGAA TGTTGTTTGT ACTTGGCTGA CCGACCGTAT GGATCTGCAA
    CTTCACATTT ATCAGCTGAA AACCCTAATT AGGGTCGTCA AGAAAACCTA CCGCGATTTC
    CGATTGCAAG GCGTCTTGGA CTCCACCTTG AACAGCAAGA CCTACGACAC CATTAGAAAC
    CGCCTCACCG TGGAAGAAGC CACAGCGTCC GTGAGCGAGG GGGGAGGCCT CCAGGGAATC
    ACCATGAAGG ACAGCGACGA GGAGGATGAG GAGGATGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815882.1
    CDS747..4748
    Translation

    Target ORF information:

    RefSeq Version XM_012960428.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ca++-dependent secretion activator (cadps), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012960428.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTTGGACC CGTCTTCCAG CGAGGAGGAA TCGGACGAGG TTGTGGAGGA GCCTGAAATC 
    AAGGAGGGTC AGGCCCCGAC CACCGGGACT CGCCTTTCCC CTAGTCGGAC CAGCGACAGC
    TCTGGGGGGC TCCAACCCAG CAGTCGGAGC AGCAGCGTCC GGCCCTCCAG CCCCAGCCCG
    TCGGTGGTGA GTGAGAAGGA GAAGGAAGAG CTGGAGAGGC TGCAGAAGGA GGAAGAGGAG
    AGGAAGAGGA AGCTTCAGCT CTATGTGTTT GTGATGAGGT GCATCGCCTA CCCCTTCAAT
    GCCAAGCAGC CCACCGACAT GGCCAGGAGG CAGCAGAAGA TAAGTAAACA GCAACTTCAG
    ACCGTCAAGG ATCGCTTTCA GGCCTTCTTC AATGGAGAAA CTCAAATTGT GGCCGATGAG
    GCGTTCATGA ATGCCGTTCA GAGCTACTAC GAGGTCTTTC TGAAGAGTGA CCGCGTGGCC
    AGAATGGTGC AAAGTGGAGG CTGCTCCGCC AATGACTCTC GGGAAGTGTT CAAGAAGCAT
    ATAGAAAAGA GGGTGCGCAG CCTCCCCGAG ATCGACGGCC TCAGCAAAGA GACAGTCCTG
    AGCTCCTGGA TGGCTAAGTT TGATGCTATT TACCGCGGGG AGGAAGATCC GCGCAAGCAG
    CAGGCCAGGA TGACGGCGAG TGCCGCGTCG GAGCTCATAC TCAGCAAAGA GCAGCTTTAC
    GAAATGTTCC AACAGATCTT GGGAATTAAA AAATTTGAGC ATCAGCTTCT GTACAACGCG
    TGCCAGCTGG ATAACCCTGA TGAGCAAGCC GCCCAGATAA GACGTGAATT AGATGGCCGC
    CTTCAGATGG CAGATCAGAT TGCAAGGGAA CGCAAATTTC CAAAATTTGT CTCCAAAGAA
    ATGGAGAACA TGTACATCGA GGAGCTGAAG TCTTCTGTGA ATCTCCTCAT GGCAAATCTG
    GAGAGCATGC CCGTGTCTAA AGGGGGCTCG GAATTTAAAC TGCAGAAGCT GAAGCGCAGC
    CACAACACGT CCATCATTGA CATGGGGGAA GAGAATGAGA ACCAACTGTC CAAGTCGGAC
    GTTGTCCTGG CTTTTAGCTT AGAGGTTGTA ATAATGGAAG TCCAAGGCTT AAAATCATTA
    GCCCCAAATC GCATCGTTTA TTGTACCATG GAAGTAGAAG GAGGGGAAAA ACTTCAAACC
    GACCAAGCGG AAGCTTCCAA ACCCATGTGG GGGACTCAAG GTGACTTCTC AACCACCCAT
    GCATTACCTG CAGTCAAAGT GAAGCTTTTC ACAGAAAGCA CCGGAGTTTT GGCTTTGGAA
    GATAAAGAAC TTGGACGGGT TGTTCTTCAC CCTACACCGA ATAGCCCCAA ACAATCAGAA
    TGGCACAAAA TGACAATATC CAAAAATTGT CCAGATCACG ATTTGAAAAT CAAATTAGCA
    GTTCGAATGG ATAAACCCCA GAACATGAAG CACTGTGGGT ATCTGTGGGC GATTGGTAAA
    AATGTCTGGA AGAGATGGAA GAAACGATTC TTTGTTCTTG TACAGGTTAG CCAATACACA
    TTTGCTATGT GCAGTTACCG AGAGAAAAAA GCAGAACCTC AGGAGCTTCT TCAGCTAGAT
    GGATACACAG TGGATTACAC CGACCCACAG CCAGGTTTGG AAGGTGGCAG GTCATTTTTC
    AACGCAGTCA AGGAAGGAGA CACTGTGATC TTTGCAAGTG ACGATGAACA AGACCGGATC
    TTATGGGTTC AAGCGATGTA CAGAGCAACG GGGCAATCTC ACAAGCCTGT GCCTCCAACC
    CAAGTTCAAA AGCTAAATGC AAAAGGAGGC AATGTACCCC AGCTGGATGC CCCAATATCC
    CAATTTTCTG GACTGAAGGA TGCTGATAGG GCACAAAAAC ATGGCATGGA TGAATTTATC
    TCGTCCAATC CCTGTAACTT TGACCACGCC ACCCTCTTTG AGATGGTTCA GCGACTCACA
    CTGGATCACA GACTCAACGA CTCGTACTCA TGTCTGGGTT GGTTCAGTCC AGGTCAGGTC
    TTTGTACTCG ATGAATATTG TGCAAGATAT GGAGTCCGAG GCTGCCATAG GCATTTGTGT
    TACCTTGGTG ATTTACTAGA ACGAGCAGAG AATGGGTCAA TGGTCGACCC AACTTTACTT
    CACTATAGTT TTGCCTTCTG CGCATCCCAC GTACATGGCA ACAGGCCTGA TGGCATCGGA
    ACTGTAACCG TGGAAGAAAA AGAGCGCTTT GAAGAGATCA AAGAACGTCT CCGCTTGCTT
    TTGGAGAATC AGATCACGCA TTTTAGGTAC TGCTTTCCAT TTGGCCGACC AGAAGGTGCT
    TTGAAAGCCA CTCTGTCATT GCTGGAAAGG GTTTTAATGA AAGACATAGT AACTCCCGTC
    CCTCAAGAAG AAGTGAAAAA CGTTATCCGT AAATGCTTAG AGCAAGCAGC CTTAGTTAAC
    TACACGAGAT TATCCGAATA TGCCAAAATC GAAGAGAATC AAAAGGACGC AGAAAATGTA
    GGCCGATTAG TAACGCCTGC CAAAAAGCTC GAAGATACAA TACGGCTCGC AGAACTTGTC
    ATTGAAGTTC TACAGCAGAA TGAGGAGCAC CATGCGGAGG GGAAAGAGGC GTTTGCTTGG
    TGGTCGGACT TGATGGTGGA ACACGCAGAG ACCTTCCTTT CGCTTTTCGC GGTGGATATG
    GATGCTGCTT TAGAGGTGCA ACCGCCAGAC AGCTGGGACA GCTTCCCTCT TTTTCAGCTA
    ATAAATGACT TCCTTCGAAG TGACTATAAT TTATGCAATG GGAAATTCCA CAAACACCTT
    CAAGATCTCT TTGCTCCACT CGTTGTGAGA TACGTCGACC TCATGGAGTC CTCAATTGCA
    CAGTCAATTC ACAGGGGCTT TGAGCGTGAA TCATGGGAAC CAGTAAAGAG TTTAACAAGT
    AATCTACCCA ATGTGAATCT ACCAAATGTG AATCTCCCTA AAGTACCAGT TACCTTACCA
    GTTAACCTCC CACAAATGCC TAGTTTTTCT GCACCGTCAT GGATGGCAGC TATATATGAT
    TCGGACAATG GATCAGCAAC TTCAGAAGAT CTATTCTGGA AGCTTGACGC TCTGCAAACG
    TTCATTCGAG ATCTTCATTG GCCCGAGGAG GAATTCGGGA AACACCTAGA GCAACGCCTT
    AAACTTATGG CTAGCGATAT GATCGAATCG TGTGTCAAAC GAACTAGAAT TGCATTCGAA
    GTCAAACTGC AGAAAACCAG TCGATCAACA GACTTCCGAG TACCGCAATC AATATGCACC
    ATGTTTAATG TTATGGTTGA TGCCAAAGCT CAGTCAACGA AACTTTGTAG CATGGAAATG
    GGCCAAGAGC ACCAGTACCA CTCCCAAATT GACGAGCTAA TTGAAGAAAC AGTTAAAGAG
    ATGATCACAC TCTTAGTGGC AAAGTTTGTC ACAATTCTAG AAGGAGTTCT GTCCAAACTA
    TCCAGATACG ATGAAGGGAC ATTATTTTCC TCCTTTCTGT CATTTACTGT GAAAGCAGCT
    TCCAAGTATG TGGATGTACC AAAACCCGGG ATGGACCTTG CCGACGCCTA CGTGACATTT
    GTGCGCCACT CTCAGGATGT CCTGCGTGAT AAGGTCAATG AGGAGATATA TATAGAAAGG
    TTATTTGATA AAAGGCTGGA TGGTAATAGT TCTGTCATGT ACCTGAGAAT ATTGGAGCAA
    TGGTATACCA GCTCAATGAA TGTTGTTTGT ACTTGGCTGA CCGACCGTAT GGATCTGCAA
    CTTCACATTT ATCAGCTGAA AACCCTAATT AGGGTCGTCA AGAAAACCTA CCGCGATTTC
    CGATTGCAAG GCGTCTTGGA CTCCACCTTG AACAGCAAGA CCTACGACAC CATTAGAAAC
    CGCCTCACCG TGGAAGAAGC CACAGCGTCC GTGAGCGAGG GGGGAGGCCT CCAGGGAATC
    ACCATGAAGG ACAGCGACGA GGAGGATGAG GAGGATGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.