rasgrp1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	rasgrp1
Entrez Gene ID	100101791
Full Name	RAS guanyl releasing protein 1
Synonyms	hrasgrp1,rasgrp
General protein information	Preferred Names RAS guanyl releasing protein 1 Names RAS guanyl-releasing protein 1 RAS guanyl releasing protein 1 (calcium and DAG-regulated) caldag-gefi caldag-gefii
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018096099.1	XP_017951588.1	RAS guanyl-releasing protein 1 isoform X1
XM_012967848.2	XP_012823302.1	RAS guanyl-releasing protein 1 isoform X3
XM_031906824.1	XP_031762684.1	RAS guanyl-releasing protein 1 isoform X1
XM_012967847.2	XP_012823301.1	RAS guanyl-releasing protein 1 isoform X2
NM_001100278.2	NP_001093748.2	RAS guanyl-releasing protein 1
XM_012967847.3	XP_012823301.1	RAS guanyl-releasing protein 1 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RASGRP1	XP_510293.2
Canis lupus familiaris (dog)	RASGRP1	XP_005638732.1
Rattus norvegicus (Norway rat)	Rasgrp1	NP_062084.2
Mus musculus (house mouse)	Rasgrp1	NP_035376.1
Homo sapiens (human)	RASGRP1	NP_005730.2
	RASGRP1	XP_001092164.1
Bos taurus (cattle)	RASGRP1	NP_001137550.1
Gallus gallus (chicken)	RASGRP1	XP_004941790.1
Danio rerio (zebrafish)	rasgrp4	NP_001038327.1
Xenopus tropicalis (tropical clawed frog)	rasgrp1	NP_001093748.2

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following rasgrp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rasgrp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa01081	NM_001100278.2 Latest version!	Xenopus tropicalis RAS guanyl releasing protein 1 (rasgrp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa30281	XM_012967848.2 Latest version!	Xenopus tropicalis RAS guanyl releasing protein 1 (calcium and DAG-regulated) (rasgrp1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa30280	XM_012967847.3 Latest version!	Xenopus tropicalis RAS guanyl releasing protein 1 (rasgrp1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa30280	XM_031906824.1 Latest version!	Xenopus tropicalis RAS guanyl releasing protein 1 (rasgrp1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa45935	XM_018096099.1 Latest version!	Xenopus tropicalis RAS guanyl releasing protein 1 (calcium and DAG-regulated) (rasgrp1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
OXa30280	XM_012967847.2	Xenopus tropicalis RAS guanyl releasing protein 1 (calcium and DAG-regulated) (rasgrp1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rasgrp1 ( NM_001100278.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001093748.2 Xenopus tropicalis RAS guanyl releasing protein 1 (rasgrp1), mRNA.

Price & Availability↑

CloneID	OXa01081
Clone ID Related Accession (Same CDS sequence)	NM_001100278.2
Accession Version	NM_001100278.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2436bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-22
Organism	Xenopus tropicalis(tropical clawed frog)
Product	RAS guanyl-releasing protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAMC03032514.1 and AAMC03032515.1. On Oct 1, 2011 this sequence version replaced NM_001100278.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC136226.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00861990, SAMN00861991 [ECO:0000348] ##Evidence-Data-END##

ATGGGATACA GACTGGGGGC TGATACTAAA CATCACTCCG ACTACTGCAG CATCTTCGTC 
ATGGGGACTG TCGGCAAGAA GAAGGACCGA CCCACTCATG GCTGTTCCAC CATCCCCAAA 
CTCGCTCTGG AGCTGAAGCA GATCATCCAC TCTACCACCC ATCCCAAAGT GCCCCCTGTC 
ACTCCCTTAC GGGTGATGAT GCCTCTTGGA AAACTCTCTA AAGGAGCCAG CTTGGATGAC 
CTGATACAGA TGTGCATTCA GGCTTTCGAT TTAGATGGCA ATATGGGGCA GAACAGCGAG 
CTGCTGCAGA TTATGCTGAC TATGCATGGC TTCCTGCTTC CCTCCACAGA ATTGCTCATG 
AAGCTCAGGA CACTATATCA GGATGCCCTG CAGAACAGAT CTTTTTCATT CTGCCTCAGG 
ATCTGCTACT TTATAAGATA CTGGGTCACA GAACTCTGGG TCATGTTCAA AATGGATGCA 
AAACTCACTC AGGCAATGGA AGAGTTCCAG GAGCTGGTGA GATCGAAAGG GGAGGAGTTA 
CACTGGCGCT TGATTGACAC TGCTCAAATA AACTCAAGGG ACTGGTCCAG AAAACTGACA 
CAAAGAATCA AACCCAACTG CAGCAAGAAG AGGAAAGTGT CTCTGCTGTT TGACCACTTG 
GAGCCACAGG AACTTGCTGA ACACTTGACC TACCTGGAGT TTAAAGCCTT TCGAAGAATA 
TCATTTTCAG ATTATCAGAA CTACATTGTC AGTGGCTGTG TGAAGGAAAA TCCAACCATG 
GAACGATCCA TTGCGCTGTG CAATGGAATC TCGCAATGGG TCCAGCTTAT GGTTCTGAGC 
CGTCCAACTC CCCAGCTCCG GGCTGAGGTG CTCACAAAGT TCATCCATGT AGCTCAGAAA 
CTGCATCAGC TGCAGAATTT CAACACCTTA ATGGCTGTCA TTGGGGGCCT TTGTCATAGC 
TCCATCTCCA GGCTCAAGGA TACGAGTGCA CATGTATCCC ATGATGTCAA CAAGGTCCTG 
AATGAAATGA CAGAGCTGCT GTCTTCTTGC AGGAATTATG ACAATTACCG ACGAGTGTAC 
AATGAGTGCA CCAACTTTAA GATTCCTATC CTGGGAGTCC ACCTGAAGGA CCTGATTGCT 
CTACATGAGG CCATGCCAGA CTTTCTAGAG GACAGCAAAA TTAATGTGCC AAAGCTTCAC 
TCTTTATATA ATCACATCAA TGAGCTCATC CAGTTGCAGA ACATTGCTCC TCCATTGGAA 
GCAAACATGG ACCTTGTACA TCTACTCACG CTCTCCCTTG ATTTATATTA TACTGAAGAT 
GAAATGTATG AGCTTTCTTA CGCACGGGAG CCAAGAAATC ACCGGGCACC GCCAGTCACA 
CCCTCCAAAC CTCCTGTGGT TGCAGACTGG GCATCAGGAG TCTCTCCAAA GCCAGACCCT 
AAAACTATCA GCAAGCATGT GCAGAGGATG GTGGATTCTG TATTTAAGAA TTATGACCTG 
GATCAAGATG GATATATATC CCAAGAAGAA TTTGAGAAGA TAGCAGCCAG CTTCCCTTTC 
TCTTTCTGCG TCATGGATAA AGACAGGGAA GGGTTAATCA GCAGGCAAGA GATAACTGCT 
TACTTCATGC GAGCCAGCTC TATTTGCTCA AAGCTTGGCC TGGGATTCCT CCATAATTTC 
CAGGAGACAA CCTATCTGCG TCCTACCTTC TGTGATAACT GCGCAGGATT TTTGTGGGGA 
GTGATCAAGC AAGGATACCG ATGCAAAGAT TGTGGAATGA ATTGCCATAA ACAGTGTAAA 
GAACTGGTGG TATTTGAATG TAAGAAGAGG TCGAAACTCT CGGTTGGAGA GAACAGTTCC 
ATGTTTGACT CTGGACAGTT AGAAGTTATT CCAGCGGGAG GCAAAGGGCA AACAAATGAC 
TGCTTGGGAG CAGAGGAAGG ACCCTATTCC TATCCTAATG GTGATGGTGA CATCCACACA 
GAGGTCAGTA AGGATCGGAC CATTATGCTT ATGGGGAGCT CAGCACAGAA GATCTCAGTA 
CGACTGCAGC CAGCTGTGAA ACACAGAGCA ACGCAGACAG AGAATGAGCC ACAAAGCCTG 
TGCCTACAAG TTCCGTCTCC ACAGCGCAGC AGGACCCCAG GCCTAACCTC CCATCTCCCT 
ATAAGCCCCA TGCCTTCCCC CTGTCCCAGT CCAGTTCCCA CACGAAAGAA GGCTTATGCC 
AAATGGGAAA ACAAGGATTC CATCCGTAAG GCAAGAGCAG AATTGAGGGG AGGCAAAGCT 
GGCATACAGG AGTTGGAGAA GGAAAAGGCC TTACTAAAGG AGGAAAACAC AACTCTAAAG 
ATCCAGCTGA AGGATGCTCA GAGACGTGTT GAGACTCTGC GGGCAGAGTT AAGGAAATAT 
GTATTGGACA GCGACGTGCA TCAGACGGGA TCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001093748.2
CDS	220..2655
Translation	MGYRLGADTKHHSDYCSIFVMGTVGKKKDRPTHGCSTIPKLALELKQIIHSTTHPKVPPVTPLRVMMPLGKLSKGASLDDLIQMCIQAFDLDGNMGQNSELLQIMLTMHGFLLPSTELLMKLRTLYQDALQNRSFSFCLRICYFIRYWVTELWVMFKMDAKLTQAMEEFQELVRSKGEELHWRLIDTAQINSRDWSRKLTQRIKPNCSKKRKVSLLFDHLEPQELAEHLTYLEFKAFRRISFSDYQNYIVSGCVKENPTMERSIALCNGISQWVQLMVLSRPTPQLRAEVLTKFIHVAQKLHQLQNFNTLMAVIGGLCHSSISRLKDTSAHVSHDVNKVLNEMTELLSSCRNYDNYRRVYNECTNFKIPILGVHLKDLIALHEAMPDFLEDSKINVPKLHSLYNHINELIQLQNIAPPLEANMDLVHLLTLSLDLYYTEDEMYELSYAREPRNHRAPPVTPSKPPVVADWASGVSPKPDPKTISKHVQRMVDSVFKNYDLDQDGYISQEEFEKIAASFPFSFCVMDKDREGLISRQEITAYFMRASSICSKLGLGFLHNFQETTYLRPTFCDNCAGFLWGVIKQGYRCKDCGMNCHKQCKELVVFECKKRSKLSVGENSSMFDSGQLEVIPAGGKGQTNDCLGAEEGPYSYPNGDGDIHTEVSKDRTIMLMGSSAQKISVRLQPAVKHRATQTENEPQSLCLQVPSPQRSRTPGLTSHLPISPMPSPCPSPVPTRKKAYAKWENKDSIRKARAELRGGKAGIQELEKEKALLKEENTTLKIQLKDAQRRVETLRAELRKYVLDSDVHQTGS

Target ORF information:

RefSeq Version	NM_001100278.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis RAS guanyl releasing protein 1 (rasgrp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001100278.2

ORF Insert Sequence:

ATGGGATACA GACTGGGGGC TGATACTAAA CATCACTCCG ACTACTGCAG CATCTTCGTC 
ATGGGGACTG TCGGCAAGAA GAAGGACCGA CCCACTCATG GCTGTTCCAC CATCCCCAAA 
CTCGCTCTGG AGCTGAAGCA GATCATCCAC TCTACCACCC ATCCCAAAGT GCCCCCTGTC 
ACTCCCTTAC GGGTGATGAT GCCTCTTGGA AAACTCTCTA AAGGAGCCAG CTTGGATGAC 
CTGATACAGA TGTGCATTCA GGCTTTCGAT TTAGATGGCA ATATGGGGCA GAACAGCGAG 
CTGCTGCAGA TTATGCTGAC TATGCATGGC TTCCTGCTTC CCTCCACAGA ATTGCTCATG 
AAGCTCAGGA CACTATATCA GGATGCCCTG CAGAACAGAT CTTTTTCATT CTGCCTCAGG 
ATCTGCTACT TTATAAGATA CTGGGTCACA GAACTCTGGG TCATGTTCAA AATGGATGCA 
AAACTCACTC AGGCAATGGA AGAGTTCCAG GAGCTGGTGA GATCGAAAGG GGAGGAGTTA 
CACTGGCGCT TGATTGACAC TGCTCAAATA AACTCAAGGG ACTGGTCCAG AAAACTGACA 
CAAAGAATCA AACCCAACTG CAGCAAGAAG AGGAAAGTGT CTCTGCTGTT TGACCACTTG 
GAGCCACAGG AACTTGCTGA ACACTTGACC TACCTGGAGT TTAAAGCCTT TCGAAGAATA 
TCATTTTCAG ATTATCAGAA CTACATTGTC AGTGGCTGTG TGAAGGAAAA TCCAACCATG 
GAACGATCCA TTGCGCTGTG CAATGGAATC TCGCAATGGG TCCAGCTTAT GGTTCTGAGC 
CGTCCAACTC CCCAGCTCCG GGCTGAGGTG CTCACAAAGT TCATCCATGT AGCTCAGAAA 
CTGCATCAGC TGCAGAATTT CAACACCTTA ATGGCTGTCA TTGGGGGCCT TTGTCATAGC 
TCCATCTCCA GGCTCAAGGA TACGAGTGCA CATGTATCCC ATGATGTCAA CAAGGTCCTG 
AATGAAATGA CAGAGCTGCT GTCTTCTTGC AGGAATTATG ACAATTACCG ACGAGTGTAC 
AATGAGTGCA CCAACTTTAA GATTCCTATC CTGGGAGTCC ACCTGAAGGA CCTGATTGCT 
CTACATGAGG CCATGCCAGA CTTTCTAGAG GACAGCAAAA TTAATGTGCC AAAGCTTCAC 
TCTTTATATA ATCACATCAA TGAGCTCATC CAGTTGCAGA ACATTGCTCC TCCATTGGAA 
GCAAACATGG ACCTTGTACA TCTACTCACG CTCTCCCTTG ATTTATATTA TACTGAAGAT 
GAAATGTATG AGCTTTCTTA CGCACGGGAG CCAAGAAATC ACCGGGCACC GCCAGTCACA 
CCCTCCAAAC CTCCTGTGGT TGCAGACTGG GCATCAGGAG TCTCTCCAAA GCCAGACCCT 
AAAACTATCA GCAAGCATGT GCAGAGGATG GTGGATTCTG TATTTAAGAA TTATGACCTG 
GATCAAGATG GATATATATC CCAAGAAGAA TTTGAGAAGA TAGCAGCCAG CTTCCCTTTC 
TCTTTCTGCG TCATGGATAA AGACAGGGAA GGGTTAATCA GCAGGCAAGA GATAACTGCT 
TACTTCATGC GAGCCAGCTC TATTTGCTCA AAGCTTGGCC TGGGATTCCT CCATAATTTC 
CAGGAGACAA CCTATCTGCG TCCTACCTTC TGTGATAACT GCGCAGGATT TTTGTGGGGA 
GTGATCAAGC AAGGATACCG ATGCAAAGAT TGTGGAATGA ATTGCCATAA ACAGTGTAAA 
GAACTGGTGG TATTTGAATG TAAGAAGAGG TCGAAACTCT CGGTTGGAGA GAACAGTTCC 
ATGTTTGACT CTGGACAGTT AGAAGTTATT CCAGCGGGAG GCAAAGGGCA AACAAATGAC 
TGCTTGGGAG CAGAGGAAGG ACCCTATTCC TATCCTAATG GTGATGGTGA CATCCACACA 
GAGGTCAGTA AGGATCGGAC CATTATGCTT ATGGGGAGCT CAGCACAGAA GATCTCAGTA 
CGACTGCAGC CAGCTGTGAA ACACAGAGCA ACGCAGACAG AGAATGAGCC ACAAAGCCTG 
TGCCTACAAG TTCCGTCTCC ACAGCGCAGC AGGACCCCAG GCCTAACCTC CCATCTCCCT 
ATAAGCCCCA TGCCTTCCCC CTGTCCCAGT CCAGTTCCCA CACGAAAGAA GGCTTATGCC 
AAATGGGAAA ACAAGGATTC CATCCGTAAG GCAAGAGCAG AATTGAGGGG AGGCAAAGCT 
GGCATACAGG AGTTGGAGAA GGAAAAGGCC TTACTAAAGG AGGAAAACAC AACTCTAAAG 
ATCCAGCTGA AGGATGCTCA GAGACGTGTT GAGACTCTGC GGGCAGAGTT AAGGAAATAT 
GTATTGGACA GCGACGTGCA TCAGACGGGA TCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rasgrp1 ( XM_012967847.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823301.1 Xenopus tropicalis RAS guanyl releasing protein 1 (calcium and DAG-regulated) (rasgrp1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa30280
Clone ID Related Accession (Same CDS sequence)	XM_031906824.1 , XM_012967847.2 , XM_012967847.3
Accession Version	XM_012967847.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2241bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	RAS guanyl-releasing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012967847.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCCTC TTGGAAAACT CTCTAAAGGA GCCAGCTTGG ATGACCTGAT ACAGATGTGC 
ATTCAGGCTT TCGATTTAGA TGGCAATATG GGGCAGAACA GCGAGCTGCT GCAGATTATG 
CTGACTATGC ATGGCTTCCT GCTTCCCTCC ACAGAATTGC TCATGAAGCT CAGGACACTA 
TATCAGGATG CCCTGCAGAA CAGATCTTTT TCATTCTGCC TCAGGATCTG CTACTTTATA 
AGATACTGGG TCACAGAACT CTGGGTCATG TTCAAAATGG ATGCAAAACT CACTCAGGCA 
ATGGAAGAGT TCCAGGAGCT GGTGAGATCG AAAGGGGAGG AGTTACACTG GCGCTTGATT 
GACACTGCTC AAATAAACTC AAGGGACTGG TCCAGAAAAC TGACACAAAG AATCAAACCC 
AACTGCAGCA AGAAGAGGAA AGTGTCTCTG CTGTTTGACC ACTTGGAGCC ACAGGAACTT 
GCTGAACACT TGACCTACCT GGAGTTTAAA GCCTTTCGAA GAATATCATT TTCAGATTAT 
CAGAACTACA TTGTCAGTGG CTGTGTGAAG GAAAATCCAA CCATGGAACG ATCCATTGCG 
CTGTGCAATG GAATCTCGCA ATGGGTCCAG CTTATGGTTC TGAGCCGTCC AACTCCCCAG 
CTCCGGGCTG AGGTGCTCAC AAAGTTCATC CATGTAGCTC AGAAACTGCA TCAGCTGCAG 
AATTTCAACA CCTTAATGGC TGTCATTGGG GGCCTTTGTC ATAGCTCCAT CTCCAGGCTC 
AAGGATACGA GTGCACATGT ATCCCATGAT GTCAACAAGG TCCTGAATGA AATGACAGAG 
CTGCTGTCTT CTTGCAGGAA TTATGACAAT TACCGACGAG TGTACAATGA GTGCACCAAC 
TTTAAGATTC CTATCCTGGG AGTCCACCTG AAGGACCTGA TTGCTCTACA TGAGGCCATG 
CCAGACTTTC TAGAGGACAG CAAAATTAAT GTGCCAAAGC TTCACTCTTT ATATAATCAC 
ATCAATGAGC TCATCCAGTT GCAGAACATT GCTCCTCCAT TGGAAGCAAA CATGGACCTT 
GTACATCTAC TCACGCTCTC CCTTGATTTA TATTATACTG AAGATGAAAT GTATGAGCTT 
TCTTACGCAC GGGAGCCAAG AAATCACCGG GCACCGCCAG TCACACCCTC CAAACCTCCT 
GTGGTTGCAG ACTGGGCATC AGGAGTCTCT CCAAAGCCAG ACCCTAAAAC TATCAGCAAG 
CATGTGCAGA GGATGGTGGA TTCTGTATTT AAGAATTATG ACCTGGATCA AGATGGATAT 
ATATCCCAAG AAGAATTTGA GAAGATAGCA GCCAGCTTCC CTTTCTCTTT CTGCGTCATG 
GATAAAGACA GGGAAGGGTT AATCAGCAGG CAAGAGATAA CTGCTTACTT CATGCGAGCC 
AGCTCTATTT GCTCAAAGCT TGGCCTGGGA TTCCTCCATA ATTTCCAGGA GACAACCTAT 
CTGCGTCCTA CCTTCTGTGA TAACTGCGCA GGATTTTTGT GGGGAGTGAT CAAGCAAGGA 
TACCGATGCA AAGATTGTGG AATGAATTGC CATAAACAGT GTAAAGAACT GGTGGTATTT 
GAATGTAAGA AGAGGTCGAA ACTCTCGGTT GGAGAGAACA GTTCCATGTT TGACTCTGGA 
CAGTTAGAAG TTATTCCAGC GGGAGGCAAA GGGCAAACAA ATGACTGCTT GGGAGCAGAG 
GAAGGACCCT ATTCCTATCC TAATGGTGAT GGTGACATCC ACACAGAGGT CAGTAAGGAT 
CGGACCATTA TGCTTATGGG GAGCTCAGCA CAGAAGATCT CAGTACGACT GCAGCCAGCT 
GTGAAACACA GAGCAACGCA GACAGAGAAT GAGCCACAAA GCCTGTGCCT ACAAGTTCCG 
TCTCCACAGC GCAGCAGGAC CCCAGGCCTA ACCTCCCATC TCCCTATAAG CCCCATGCCT 
TCCCCCTGTC CCAGTCCAGT TCCCACACGA AAGAAGGCTT ATGCCAAATG GGAAAACAAG 
GATTCCATCC GTAAGGCAAG AGCAGAATTG AGGGGAGGCA AAGCTGGCAT ACAGGAGTTG 
GAGAAGGAAA AGGCCTTACT AAAGGAGGAA AACACAACTC TAAAGATCCA GCTGAAGGAT 
GCTCAGAGAC GTGTTGAGAC TCTGCGGGCA GAGTTAAGGA AATATGTATT GGACAGCGAC 
GTGCATCAGA CGGGATCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012823301.1
CDS	288..2528
Translation	MMPLGKLSKGASLDDLIQMCIQAFDLDGNMGQNSELLQIMLTMHGFLLPSTELLMKLRTLYQDALQNRSFSFCLRICYFIRYWVTELWVMFKMDAKLTQAMEEFQELVRSKGEELHWRLIDTAQINSRDWSRKLTQRIKPNCSKKRKVSLLFDHLEPQELAEHLTYLEFKAFRRISFSDYQNYIVSGCVKENPTMERSIALCNGISQWVQLMVLSRPTPQLRAEVLTKFIHVAQKLHQLQNFNTLMAVIGGLCHSSISRLKDTSAHVSHDVNKVLNEMTELLSSCRNYDNYRRVYNECTNFKIPILGVHLKDLIALHEAMPDFLEDSKINVPKLHSLYNHINELIQLQNIAPPLEANMDLVHLLTLSLDLYYTEDEMYELSYAREPRNHRAPPVTPSKPPVVADWASGVSPKPDPKTISKHVQRMVDSVFKNYDLDQDGYISQEEFEKIAASFPFSFCVMDKDREGLISRQEITAYFMRASSICSKLGLGFLHNFQETTYLRPTFCDNCAGFLWGVIKQGYRCKDCGMNCHKQCKELVVFECKKRSKLSVGENSSMFDSGQLEVIPAGGKGQTNDCLGAEEGPYSYPNGDGDIHTEVSKDRTIMLMGSSAQKISVRLQPAVKHRATQTENEPQSLCLQVPSPQRSRTPGLTSHLPISPMPSPCPSPVPTRKKAYAKWENKDSIRKARAELRGGKAGIQELEKEKALLKEENTTLKIQLKDAQRRVETLRAELRKYVLDSDVHQTGS

Target ORF information:

RefSeq Version	XM_012967847.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis RAS guanyl releasing protein 1 (calcium and DAG-regulated) (rasgrp1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967847.2

ORF Insert Sequence:

ATGATGCCTC TTGGAAAACT CTCTAAAGGA GCCAGCTTGG ATGACCTGAT ACAGATGTGC 
ATTCAGGCTT TCGATTTAGA TGGCAATATG GGGCAGAACA GCGAGCTGCT GCAGATTATG 
CTGACTATGC ATGGCTTCCT GCTTCCCTCC ACAGAATTGC TCATGAAGCT CAGGACACTA 
TATCAGGATG CCCTGCAGAA CAGATCTTTT TCATTCTGCC TCAGGATCTG CTACTTTATA 
AGATACTGGG TCACAGAACT CTGGGTCATG TTCAAAATGG ATGCAAAACT CACTCAGGCA 
ATGGAAGAGT TCCAGGAGCT GGTGAGATCG AAAGGGGAGG AGTTACACTG GCGCTTGATT 
GACACTGCTC AAATAAACTC AAGGGACTGG TCCAGAAAAC TGACACAAAG AATCAAACCC 
AACTGCAGCA AGAAGAGGAA AGTGTCTCTG CTGTTTGACC ACTTGGAGCC ACAGGAACTT 
GCTGAACACT TGACCTACCT GGAGTTTAAA GCCTTTCGAA GAATATCATT TTCAGATTAT 
CAGAACTACA TTGTCAGTGG CTGTGTGAAG GAAAATCCAA CCATGGAACG ATCCATTGCG 
CTGTGCAATG GAATCTCGCA ATGGGTCCAG CTTATGGTTC TGAGCCGTCC AACTCCCCAG 
CTCCGGGCTG AGGTGCTCAC AAAGTTCATC CATGTAGCTC AGAAACTGCA TCAGCTGCAG 
AATTTCAACA CCTTAATGGC TGTCATTGGG GGCCTTTGTC ATAGCTCCAT CTCCAGGCTC 
AAGGATACGA GTGCACATGT ATCCCATGAT GTCAACAAGG TCCTGAATGA AATGACAGAG 
CTGCTGTCTT CTTGCAGGAA TTATGACAAT TACCGACGAG TGTACAATGA GTGCACCAAC 
TTTAAGATTC CTATCCTGGG AGTCCACCTG AAGGACCTGA TTGCTCTACA TGAGGCCATG 
CCAGACTTTC TAGAGGACAG CAAAATTAAT GTGCCAAAGC TTCACTCTTT ATATAATCAC 
ATCAATGAGC TCATCCAGTT GCAGAACATT GCTCCTCCAT TGGAAGCAAA CATGGACCTT 
GTACATCTAC TCACGCTCTC CCTTGATTTA TATTATACTG AAGATGAAAT GTATGAGCTT 
TCTTACGCAC GGGAGCCAAG AAATCACCGG GCACCGCCAG TCACACCCTC CAAACCTCCT 
GTGGTTGCAG ACTGGGCATC AGGAGTCTCT CCAAAGCCAG ACCCTAAAAC TATCAGCAAG 
CATGTGCAGA GGATGGTGGA TTCTGTATTT AAGAATTATG ACCTGGATCA AGATGGATAT 
ATATCCCAAG AAGAATTTGA GAAGATAGCA GCCAGCTTCC CTTTCTCTTT CTGCGTCATG 
GATAAAGACA GGGAAGGGTT AATCAGCAGG CAAGAGATAA CTGCTTACTT CATGCGAGCC 
AGCTCTATTT GCTCAAAGCT TGGCCTGGGA TTCCTCCATA ATTTCCAGGA GACAACCTAT 
CTGCGTCCTA CCTTCTGTGA TAACTGCGCA GGATTTTTGT GGGGAGTGAT CAAGCAAGGA 
TACCGATGCA AAGATTGTGG AATGAATTGC CATAAACAGT GTAAAGAACT GGTGGTATTT 
GAATGTAAGA AGAGGTCGAA ACTCTCGGTT GGAGAGAACA GTTCCATGTT TGACTCTGGA 
CAGTTAGAAG TTATTCCAGC GGGAGGCAAA GGGCAAACAA ATGACTGCTT GGGAGCAGAG 
GAAGGACCCT ATTCCTATCC TAATGGTGAT GGTGACATCC ACACAGAGGT CAGTAAGGAT 
CGGACCATTA TGCTTATGGG GAGCTCAGCA CAGAAGATCT CAGTACGACT GCAGCCAGCT 
GTGAAACACA GAGCAACGCA GACAGAGAAT GAGCCACAAA GCCTGTGCCT ACAAGTTCCG 
TCTCCACAGC GCAGCAGGAC CCCAGGCCTA ACCTCCCATC TCCCTATAAG CCCCATGCCT 
TCCCCCTGTC CCAGTCCAGT TCCCACACGA AAGAAGGCTT ATGCCAAATG GGAAAACAAG 
GATTCCATCC GTAAGGCAAG AGCAGAATTG AGGGGAGGCA AAGCTGGCAT ACAGGAGTTG 
GAGAAGGAAA AGGCCTTACT AAAGGAGGAA AACACAACTC TAAAGATCCA GCTGAAGGAT 
GCTCAGAGAC GTGTTGAGAC TCTGCGGGCA GAGTTAAGGA AATATGTATT GGACAGCGAC 
GTGCATCAGA CGGGATCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rasgrp1 ( XM_012967848.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823302.1 Xenopus tropicalis RAS guanyl releasing protein 1 (calcium and DAG-regulated) (rasgrp1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa30281
Clone ID Related Accession (Same CDS sequence)	XM_012967848.2
Accession Version	XM_012967848.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	RAS guanyl-releasing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012967848.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAGA ACAGCGAGCT GCTGCAGATT ATGCTGACTA TGCATGGCTT CCTGCTTCCC 
TCCACAGAAT TGCTCATGAA GCTCAGGACA CTATATCAGG ATGCCCTGCA GAACAGATCT 
TTTTCATTCT GCCTCAGGAT CTGCTACTTT ATAAGATACT GGGTCACAGA ACTCTGGGTC 
ATGTTCAAAA TGGATGCAAA ACTCACTCAG GCAATGGAAG AGTTCCAGGA GCTGGTGAGA 
TCGAAAGGGG AGGAGTTACA CTGGCGCTTG ATTGACACTG CTCAAATAAA CTCAAGGGAC 
TGGTCCAGAA AACTGACACA AAGAATCAAA CCCAACTGCA GCAAGAAGAG GAAAGTGTCT 
CTGCTGTTTG ACCACTTGGA GCCACAGGAA CTTGCTGAAC ACTTGACCTA CCTGGAGTTT 
AAAGCCTTTC GAAGAATATC ATTTTCAGAT TATCAGAACT ACATTGTCAG TGGCTGTGTG 
AAGGAAAATC CAACCATGGA ACGATCCATT GCGCTGTGCA ATGGAATCTC GCAATGGGTC 
CAGCTTATGG TTCTGAGCCG TCCAACTCCC CAGCTCCGGG CTGAGGTGCT CACAAAGTTC 
ATCCATGTAG CTCAGAAACT GCATCAGCTG CAGAATTTCA ACACCTTAAT GGCTGTCATT 
GGGGGCCTTT GTCATAGCTC CATCTCCAGG CTCAAGGATA CGAGTGCACA TGTATCCCAT 
GATGTCAACA AGGTCCTGAA TGAAATGACA GAGCTGCTGT CTTCTTGCAG GAATTATGAC 
AATTACCGAC GAGTGTACAA TGAGTGCACC AACTTTAAGA TTCCTATCCT GGGAGTCCAC 
CTGAAGGACC TGATTGCTCT ACATGAGGCC ATGCCAGACT TTCTAGAGGA CAGCAAAATT 
AATGTGCCAA AGCTTCACTC TTTATATAAT CACATCAATG AGCTCATCCA GTTGCAGAAC 
ATTGCTCCTC CATTGGAAGC AAACATGGAC CTTGTACATC TACTCACGCT CTCCCTTGAT 
TTATATTATA CTGAAGATGA AATGTATGAG CTTTCTTACG CACGGGAGCC AAGAAATCAC 
CGGGCACCGC CAGTCACACC CTCCAAACCT CCTGTGGTTG CAGACTGGGC ATCAGGAGTC 
TCTCCAAAGC CAGACCCTAA AACTATCAGC AAGCATGTGC AGAGGATGGT GGATTCTGTA 
TTTAAGAATT ATGACCTGGA TCAAGATGGA TATATATCCC AAGAAGAATT TGAGAAGATA 
GCAGCCAGCT TCCCTTTCTC TTTCTGCGTC ATGGATAAAG ACAGGGAAGG GTTAATCAGC 
AGGCAAGAGA TAACTGCTTA CTTCATGCGA GCCAGCTCTA TTTGCTCAAA GCTTGGCCTG 
GGATTCCTCC ATAATTTCCA GGAGACAACC TATCTGCGTC CTACCTTCTG TGATAACTGC 
GCAGGATTTT TGTGGGGAGT GATCAAGCAA GGATACCGAT GCAAAGATTG TGGAATGAAT 
TGCCATAAAC AGTGTAAAGA ACTGGTGGTA TTTGAATGTA AGAAGAGGTC GAAACTCTCG 
GTTGGAGAGA ACAGTTCCAT GTTTGACTCT GGACAGTTAG AAGTTATTCC AGCGGGAGGC 
AAAGGGCAAA CAAATGACTG CTTGGGAGCA GAGGAAGGAC CCTATTCCTA TCCTAATGGT 
GATGGTGACA TCCACACAGA GGTCAGTAAG GATCGGACCA TTATGCTTAT GGGGAGCTCA 
GCACAGAAGA TCTCAGTACG ACTGCAGCCA GCTGTGAAAC ACAGAGCAAC GCAGACAGAG 
AATGAGCCAC AAAGCCTGTG CCTACAAGTT CCGTCTCCAC AGCGCAGCAG GACCCCAGGC 
CTAACCTCCC ATCTCCCTAT AAGCCCCATG CCTTCCCCCT GTCCCAGTCC AGTTCCCACA 
CGAAAGAAGG CTTATGCCAA ATGGGAAAAC AAGGATTCCA TCCGTAAGGC AAGAGCAGAA 
TTGAGGGGAG GCAAAGCTGG CATACAGGAG TTGGAGAAGG AAAAGGCCTT ACTAAAGGAG 
GAAAACACAA CTCTAAAGAT CCAGCTGAAG GATGCTCAGA GACGTGTTGA GACTCTGCGG 
GCAGAGTTAA GGAAATATGT ATTGGACAGC GACGTGCATC AGACGGGATC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012823302.1
CDS	209..2362
Translation	MGQNSELLQIMLTMHGFLLPSTELLMKLRTLYQDALQNRSFSFCLRICYFIRYWVTELWVMFKMDAKLTQAMEEFQELVRSKGEELHWRLIDTAQINSRDWSRKLTQRIKPNCSKKRKVSLLFDHLEPQELAEHLTYLEFKAFRRISFSDYQNYIVSGCVKENPTMERSIALCNGISQWVQLMVLSRPTPQLRAEVLTKFIHVAQKLHQLQNFNTLMAVIGGLCHSSISRLKDTSAHVSHDVNKVLNEMTELLSSCRNYDNYRRVYNECTNFKIPILGVHLKDLIALHEAMPDFLEDSKINVPKLHSLYNHINELIQLQNIAPPLEANMDLVHLLTLSLDLYYTEDEMYELSYAREPRNHRAPPVTPSKPPVVADWASGVSPKPDPKTISKHVQRMVDSVFKNYDLDQDGYISQEEFEKIAASFPFSFCVMDKDREGLISRQEITAYFMRASSICSKLGLGFLHNFQETTYLRPTFCDNCAGFLWGVIKQGYRCKDCGMNCHKQCKELVVFECKKRSKLSVGENSSMFDSGQLEVIPAGGKGQTNDCLGAEEGPYSYPNGDGDIHTEVSKDRTIMLMGSSAQKISVRLQPAVKHRATQTENEPQSLCLQVPSPQRSRTPGLTSHLPISPMPSPCPSPVPTRKKAYAKWENKDSIRKARAELRGGKAGIQELEKEKALLKEENTTLKIQLKDAQRRVETLRAELRKYVLDSDVHQTGS

Target ORF information:

RefSeq Version	XM_012967848.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis RAS guanyl releasing protein 1 (calcium and DAG-regulated) (rasgrp1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967848.2

ORF Insert Sequence:

ATGGGGCAGA ACAGCGAGCT GCTGCAGATT ATGCTGACTA TGCATGGCTT CCTGCTTCCC 
TCCACAGAAT TGCTCATGAA GCTCAGGACA CTATATCAGG ATGCCCTGCA GAACAGATCT 
TTTTCATTCT GCCTCAGGAT CTGCTACTTT ATAAGATACT GGGTCACAGA ACTCTGGGTC 
ATGTTCAAAA TGGATGCAAA ACTCACTCAG GCAATGGAAG AGTTCCAGGA GCTGGTGAGA 
TCGAAAGGGG AGGAGTTACA CTGGCGCTTG ATTGACACTG CTCAAATAAA CTCAAGGGAC 
TGGTCCAGAA AACTGACACA AAGAATCAAA CCCAACTGCA GCAAGAAGAG GAAAGTGTCT 
CTGCTGTTTG ACCACTTGGA GCCACAGGAA CTTGCTGAAC ACTTGACCTA CCTGGAGTTT 
AAAGCCTTTC GAAGAATATC ATTTTCAGAT TATCAGAACT ACATTGTCAG TGGCTGTGTG 
AAGGAAAATC CAACCATGGA ACGATCCATT GCGCTGTGCA ATGGAATCTC GCAATGGGTC 
CAGCTTATGG TTCTGAGCCG TCCAACTCCC CAGCTCCGGG CTGAGGTGCT CACAAAGTTC 
ATCCATGTAG CTCAGAAACT GCATCAGCTG CAGAATTTCA ACACCTTAAT GGCTGTCATT 
GGGGGCCTTT GTCATAGCTC CATCTCCAGG CTCAAGGATA CGAGTGCACA TGTATCCCAT 
GATGTCAACA AGGTCCTGAA TGAAATGACA GAGCTGCTGT CTTCTTGCAG GAATTATGAC 
AATTACCGAC GAGTGTACAA TGAGTGCACC AACTTTAAGA TTCCTATCCT GGGAGTCCAC 
CTGAAGGACC TGATTGCTCT ACATGAGGCC ATGCCAGACT TTCTAGAGGA CAGCAAAATT 
AATGTGCCAA AGCTTCACTC TTTATATAAT CACATCAATG AGCTCATCCA GTTGCAGAAC 
ATTGCTCCTC CATTGGAAGC AAACATGGAC CTTGTACATC TACTCACGCT CTCCCTTGAT 
TTATATTATA CTGAAGATGA AATGTATGAG CTTTCTTACG CACGGGAGCC AAGAAATCAC 
CGGGCACCGC CAGTCACACC CTCCAAACCT CCTGTGGTTG CAGACTGGGC ATCAGGAGTC 
TCTCCAAAGC CAGACCCTAA AACTATCAGC AAGCATGTGC AGAGGATGGT GGATTCTGTA 
TTTAAGAATT ATGACCTGGA TCAAGATGGA TATATATCCC AAGAAGAATT TGAGAAGATA 
GCAGCCAGCT TCCCTTTCTC TTTCTGCGTC ATGGATAAAG ACAGGGAAGG GTTAATCAGC 
AGGCAAGAGA TAACTGCTTA CTTCATGCGA GCCAGCTCTA TTTGCTCAAA GCTTGGCCTG 
GGATTCCTCC ATAATTTCCA GGAGACAACC TATCTGCGTC CTACCTTCTG TGATAACTGC 
GCAGGATTTT TGTGGGGAGT GATCAAGCAA GGATACCGAT GCAAAGATTG TGGAATGAAT 
TGCCATAAAC AGTGTAAAGA ACTGGTGGTA TTTGAATGTA AGAAGAGGTC GAAACTCTCG 
GTTGGAGAGA ACAGTTCCAT GTTTGACTCT GGACAGTTAG AAGTTATTCC AGCGGGAGGC 
AAAGGGCAAA CAAATGACTG CTTGGGAGCA GAGGAAGGAC CCTATTCCTA TCCTAATGGT 
GATGGTGACA TCCACACAGA GGTCAGTAAG GATCGGACCA TTATGCTTAT GGGGAGCTCA 
GCACAGAAGA TCTCAGTACG ACTGCAGCCA GCTGTGAAAC ACAGAGCAAC GCAGACAGAG 
AATGAGCCAC AAAGCCTGTG CCTACAAGTT CCGTCTCCAC AGCGCAGCAG GACCCCAGGC 
CTAACCTCCC ATCTCCCTAT AAGCCCCATG CCTTCCCCCT GTCCCAGTCC AGTTCCCACA 
CGAAAGAAGG CTTATGCCAA ATGGGAAAAC AAGGATTCCA TCCGTAAGGC AAGAGCAGAA 
TTGAGGGGAG GCAAAGCTGG CATACAGGAG TTGGAGAAGG AAAAGGCCTT ACTAAAGGAG 
GAAAACACAA CTCTAAAGAT CCAGCTGAAG GATGCTCAGA GACGTGTTGA GACTCTGCGG 
GCAGAGTTAA GGAAATATGT ATTGGACAGC GACGTGCATC AGACGGGATC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rasgrp1 ( XM_031906824.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031762684.1 Xenopus tropicalis RAS guanyl releasing protein 1 (rasgrp1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa30280
Clone ID Related Accession (Same CDS sequence)	XM_031906824.1 , XM_012967847.2 , XM_012967847.3
Accession Version	XM_031906824.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2241bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	RAS guanyl-releasing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCCTC TTGGAAAACT CTCTAAAGGA GCCAGCTTGG ATGACCTGAT ACAGATGTGC 
ATTCAGGCTT TCGATTTAGA TGGCAATATG GGGCAGAACA GCGAGCTGCT GCAGATTATG 
CTGACTATGC ATGGCTTCCT GCTTCCCTCC ACAGAATTGC TCATGAAGCT CAGGACACTA 
TATCAGGATG CCCTGCAGAA CAGATCTTTT TCATTCTGCC TCAGGATCTG CTACTTTATA 
AGATACTGGG TCACAGAACT CTGGGTCATG TTCAAAATGG ATGCAAAACT CACTCAGGCA 
ATGGAAGAGT TCCAGGAGCT GGTGAGATCG AAAGGGGAGG AGTTACACTG GCGCTTGATT 
GACACTGCTC AAATAAACTC AAGGGACTGG TCCAGAAAAC TGACACAAAG AATCAAACCC 
AACTGCAGCA AGAAGAGGAA AGTGTCTCTG CTGTTTGACC ACTTGGAGCC ACAGGAACTT 
GCTGAACACT TGACCTACCT GGAGTTTAAA GCCTTTCGAA GAATATCATT TTCAGATTAT 
CAGAACTACA TTGTCAGTGG CTGTGTGAAG GAAAATCCAA CCATGGAACG ATCCATTGCG 
CTGTGCAATG GAATCTCGCA ATGGGTCCAG CTTATGGTTC TGAGCCGTCC AACTCCCCAG 
CTCCGGGCTG AGGTGCTCAC AAAGTTCATC CATGTAGCTC AGAAACTGCA TCAGCTGCAG 
AATTTCAACA CCTTAATGGC TGTCATTGGG GGCCTTTGTC ATAGCTCCAT CTCCAGGCTC 
AAGGATACGA GTGCACATGT ATCCCATGAT GTCAACAAGG TCCTGAATGA AATGACAGAG 
CTGCTGTCTT CTTGCAGGAA TTATGACAAT TACCGACGAG TGTACAATGA GTGCACCAAC 
TTTAAGATTC CTATCCTGGG AGTCCACCTG AAGGACCTGA TTGCTCTACA TGAGGCCATG 
CCAGACTTTC TAGAGGACAG CAAAATTAAT GTGCCAAAGC TTCACTCTTT ATATAATCAC 
ATCAATGAGC TCATCCAGTT GCAGAACATT GCTCCTCCAT TGGAAGCAAA CATGGACCTT 
GTACATCTAC TCACGCTCTC CCTTGATTTA TATTATACTG AAGATGAAAT GTATGAGCTT 
TCTTACGCAC GGGAGCCAAG AAATCACCGG GCACCGCCAG TCACACCCTC CAAACCTCCT 
GTGGTTGCAG ACTGGGCATC AGGAGTCTCT CCAAAGCCAG ACCCTAAAAC TATCAGCAAG 
CATGTGCAGA GGATGGTGGA TTCTGTATTT AAGAATTATG ACCTGGATCA AGATGGATAT 
ATATCCCAAG AAGAATTTGA GAAGATAGCA GCCAGCTTCC CTTTCTCTTT CTGCGTCATG 
GATAAAGACA GGGAAGGGTT AATCAGCAGG CAAGAGATAA CTGCTTACTT CATGCGAGCC 
AGCTCTATTT GCTCAAAGCT TGGCCTGGGA TTCCTCCATA ATTTCCAGGA GACAACCTAT 
CTGCGTCCTA CCTTCTGTGA TAACTGCGCA GGATTTTTGT GGGGAGTGAT CAAGCAAGGA 
TACCGATGCA AAGATTGTGG AATGAATTGC CATAAACAGT GTAAAGAACT GGTGGTATTT 
GAATGTAAGA AGAGGTCGAA ACTCTCGGTT GGAGAGAACA GTTCCATGTT TGACTCTGGA 
CAGTTAGAAG TTATTCCAGC GGGAGGCAAA GGGCAAACAA ATGACTGCTT GGGAGCAGAG 
GAAGGACCCT ATTCCTATCC TAATGGTGAT GGTGACATCC ACACAGAGGT CAGTAAGGAT 
CGGACCATTA TGCTTATGGG GAGCTCAGCA CAGAAGATCT CAGTACGACT GCAGCCAGCT 
GTGAAACACA GAGCAACGCA GACAGAGAAT GAGCCACAAA GCCTGTGCCT ACAAGTTCCG 
TCTCCACAGC GCAGCAGGAC CCCAGGCCTA ACCTCCCATC TCCCTATAAG CCCCATGCCT 
TCCCCCTGTC CCAGTCCAGT TCCCACACGA AAGAAGGCTT ATGCCAAATG GGAAAACAAG 
GATTCCATCC GTAAGGCAAG AGCAGAATTG AGGGGAGGCA AAGCTGGCAT ACAGGAGTTG 
GAGAAGGAAA AGGCCTTACT AAAGGAGGAA AACACAACTC TAAAGATCCA GCTGAAGGAT 
GCTCAGAGAC GTGTTGAGAC TCTGCGGGCA GAGTTAAGGA AATATGTATT GGACAGCGAC 
GTGCATCAGA CGGGATCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031762684.1
CDS	281..2521
Translation	MMPLGKLSKGASLDDLIQMCIQAFDLDGNMGQNSELLQIMLTMHGFLLPSTELLMKLRTLYQDALQNRSFSFCLRICYFIRYWVTELWVMFKMDAKLTQAMEEFQELVRSKGEELHWRLIDTAQINSRDWSRKLTQRIKPNCSKKRKVSLLFDHLEPQELAEHLTYLEFKAFRRISFSDYQNYIVSGCVKENPTMERSIALCNGISQWVQLMVLSRPTPQLRAEVLTKFIHVAQKLHQLQNFNTLMAVIGGLCHSSISRLKDTSAHVSHDVNKVLNEMTELLSSCRNYDNYRRVYNECTNFKIPILGVHLKDLIALHEAMPDFLEDSKINVPKLHSLYNHINELIQLQNIAPPLEANMDLVHLLTLSLDLYYTEDEMYELSYAREPRNHRAPPVTPSKPPVVADWASGVSPKPDPKTISKHVQRMVDSVFKNYDLDQDGYISQEEFEKIAASFPFSFCVMDKDREGLISRQEITAYFMRASSICSKLGLGFLHNFQETTYLRPTFCDNCAGFLWGVIKQGYRCKDCGMNCHKQCKELVVFECKKRSKLSVGENSSMFDSGQLEVIPAGGKGQTNDCLGAEEGPYSYPNGDGDIHTEVSKDRTIMLMGSSAQKISVRLQPAVKHRATQTENEPQSLCLQVPSPQRSRTPGLTSHLPISPMPSPCPSPVPTRKKAYAKWENKDSIRKARAELRGGKAGIQELEKEKALLKEENTTLKIQLKDAQRRVETLRAELRKYVLDSDVHQTGS

Target ORF information:

RefSeq Version	XM_031906824.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis RAS guanyl releasing protein 1 (rasgrp1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031906824.1

ORF Insert Sequence:

ATGATGCCTC TTGGAAAACT CTCTAAAGGA GCCAGCTTGG ATGACCTGAT ACAGATGTGC 
ATTCAGGCTT TCGATTTAGA TGGCAATATG GGGCAGAACA GCGAGCTGCT GCAGATTATG 
CTGACTATGC ATGGCTTCCT GCTTCCCTCC ACAGAATTGC TCATGAAGCT CAGGACACTA 
TATCAGGATG CCCTGCAGAA CAGATCTTTT TCATTCTGCC TCAGGATCTG CTACTTTATA 
AGATACTGGG TCACAGAACT CTGGGTCATG TTCAAAATGG ATGCAAAACT CACTCAGGCA 
ATGGAAGAGT TCCAGGAGCT GGTGAGATCG AAAGGGGAGG AGTTACACTG GCGCTTGATT 
GACACTGCTC AAATAAACTC AAGGGACTGG TCCAGAAAAC TGACACAAAG AATCAAACCC 
AACTGCAGCA AGAAGAGGAA AGTGTCTCTG CTGTTTGACC ACTTGGAGCC ACAGGAACTT 
GCTGAACACT TGACCTACCT GGAGTTTAAA GCCTTTCGAA GAATATCATT TTCAGATTAT 
CAGAACTACA TTGTCAGTGG CTGTGTGAAG GAAAATCCAA CCATGGAACG ATCCATTGCG 
CTGTGCAATG GAATCTCGCA ATGGGTCCAG CTTATGGTTC TGAGCCGTCC AACTCCCCAG 
CTCCGGGCTG AGGTGCTCAC AAAGTTCATC CATGTAGCTC AGAAACTGCA TCAGCTGCAG 
AATTTCAACA CCTTAATGGC TGTCATTGGG GGCCTTTGTC ATAGCTCCAT CTCCAGGCTC 
AAGGATACGA GTGCACATGT ATCCCATGAT GTCAACAAGG TCCTGAATGA AATGACAGAG 
CTGCTGTCTT CTTGCAGGAA TTATGACAAT TACCGACGAG TGTACAATGA GTGCACCAAC 
TTTAAGATTC CTATCCTGGG AGTCCACCTG AAGGACCTGA TTGCTCTACA TGAGGCCATG 
CCAGACTTTC TAGAGGACAG CAAAATTAAT GTGCCAAAGC TTCACTCTTT ATATAATCAC 
ATCAATGAGC TCATCCAGTT GCAGAACATT GCTCCTCCAT TGGAAGCAAA CATGGACCTT 
GTACATCTAC TCACGCTCTC CCTTGATTTA TATTATACTG AAGATGAAAT GTATGAGCTT 
TCTTACGCAC GGGAGCCAAG AAATCACCGG GCACCGCCAG TCACACCCTC CAAACCTCCT 
GTGGTTGCAG ACTGGGCATC AGGAGTCTCT CCAAAGCCAG ACCCTAAAAC TATCAGCAAG 
CATGTGCAGA GGATGGTGGA TTCTGTATTT AAGAATTATG ACCTGGATCA AGATGGATAT 
ATATCCCAAG AAGAATTTGA GAAGATAGCA GCCAGCTTCC CTTTCTCTTT CTGCGTCATG 
GATAAAGACA GGGAAGGGTT AATCAGCAGG CAAGAGATAA CTGCTTACTT CATGCGAGCC 
AGCTCTATTT GCTCAAAGCT TGGCCTGGGA TTCCTCCATA ATTTCCAGGA GACAACCTAT 
CTGCGTCCTA CCTTCTGTGA TAACTGCGCA GGATTTTTGT GGGGAGTGAT CAAGCAAGGA 
TACCGATGCA AAGATTGTGG AATGAATTGC CATAAACAGT GTAAAGAACT GGTGGTATTT 
GAATGTAAGA AGAGGTCGAA ACTCTCGGTT GGAGAGAACA GTTCCATGTT TGACTCTGGA 
CAGTTAGAAG TTATTCCAGC GGGAGGCAAA GGGCAAACAA ATGACTGCTT GGGAGCAGAG 
GAAGGACCCT ATTCCTATCC TAATGGTGAT GGTGACATCC ACACAGAGGT CAGTAAGGAT 
CGGACCATTA TGCTTATGGG GAGCTCAGCA CAGAAGATCT CAGTACGACT GCAGCCAGCT 
GTGAAACACA GAGCAACGCA GACAGAGAAT GAGCCACAAA GCCTGTGCCT ACAAGTTCCG 
TCTCCACAGC GCAGCAGGAC CCCAGGCCTA ACCTCCCATC TCCCTATAAG CCCCATGCCT 
TCCCCCTGTC CCAGTCCAGT TCCCACACGA AAGAAGGCTT ATGCCAAATG GGAAAACAAG 
GATTCCATCC GTAAGGCAAG AGCAGAATTG AGGGGAGGCA AAGCTGGCAT ACAGGAGTTG 
GAGAAGGAAA AGGCCTTACT AAAGGAGGAA AACACAACTC TAAAGATCCA GCTGAAGGAT 
GCTCAGAGAC GTGTTGAGAC TCTGCGGGCA GAGTTAAGGA AATATGTATT GGACAGCGAC 
GTGCATCAGA CGGGATCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rasgrp1 ( XM_012967847.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823301.1 Xenopus tropicalis RAS guanyl releasing protein 1 (rasgrp1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa30280
Clone ID Related Accession (Same CDS sequence)	XM_031906824.1 , XM_012967847.2 , XM_012967847.3
Accession Version	XM_012967847.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2241bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	RAS guanyl-releasing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012967847.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCCTC TTGGAAAACT CTCTAAAGGA GCCAGCTTGG ATGACCTGAT ACAGATGTGC 
ATTCAGGCTT TCGATTTAGA TGGCAATATG GGGCAGAACA GCGAGCTGCT GCAGATTATG 
CTGACTATGC ATGGCTTCCT GCTTCCCTCC ACAGAATTGC TCATGAAGCT CAGGACACTA 
TATCAGGATG CCCTGCAGAA CAGATCTTTT TCATTCTGCC TCAGGATCTG CTACTTTATA 
AGATACTGGG TCACAGAACT CTGGGTCATG TTCAAAATGG ATGCAAAACT CACTCAGGCA 
ATGGAAGAGT TCCAGGAGCT GGTGAGATCG AAAGGGGAGG AGTTACACTG GCGCTTGATT 
GACACTGCTC AAATAAACTC AAGGGACTGG TCCAGAAAAC TGACACAAAG AATCAAACCC 
AACTGCAGCA AGAAGAGGAA AGTGTCTCTG CTGTTTGACC ACTTGGAGCC ACAGGAACTT 
GCTGAACACT TGACCTACCT GGAGTTTAAA GCCTTTCGAA GAATATCATT TTCAGATTAT 
CAGAACTACA TTGTCAGTGG CTGTGTGAAG GAAAATCCAA CCATGGAACG ATCCATTGCG 
CTGTGCAATG GAATCTCGCA ATGGGTCCAG CTTATGGTTC TGAGCCGTCC AACTCCCCAG 
CTCCGGGCTG AGGTGCTCAC AAAGTTCATC CATGTAGCTC AGAAACTGCA TCAGCTGCAG 
AATTTCAACA CCTTAATGGC TGTCATTGGG GGCCTTTGTC ATAGCTCCAT CTCCAGGCTC 
AAGGATACGA GTGCACATGT ATCCCATGAT GTCAACAAGG TCCTGAATGA AATGACAGAG 
CTGCTGTCTT CTTGCAGGAA TTATGACAAT TACCGACGAG TGTACAATGA GTGCACCAAC 
TTTAAGATTC CTATCCTGGG AGTCCACCTG AAGGACCTGA TTGCTCTACA TGAGGCCATG 
CCAGACTTTC TAGAGGACAG CAAAATTAAT GTGCCAAAGC TTCACTCTTT ATATAATCAC 
ATCAATGAGC TCATCCAGTT GCAGAACATT GCTCCTCCAT TGGAAGCAAA CATGGACCTT 
GTACATCTAC TCACGCTCTC CCTTGATTTA TATTATACTG AAGATGAAAT GTATGAGCTT 
TCTTACGCAC GGGAGCCAAG AAATCACCGG GCACCGCCAG TCACACCCTC CAAACCTCCT 
GTGGTTGCAG ACTGGGCATC AGGAGTCTCT CCAAAGCCAG ACCCTAAAAC TATCAGCAAG 
CATGTGCAGA GGATGGTGGA TTCTGTATTT AAGAATTATG ACCTGGATCA AGATGGATAT 
ATATCCCAAG AAGAATTTGA GAAGATAGCA GCCAGCTTCC CTTTCTCTTT CTGCGTCATG 
GATAAAGACA GGGAAGGGTT AATCAGCAGG CAAGAGATAA CTGCTTACTT CATGCGAGCC 
AGCTCTATTT GCTCAAAGCT TGGCCTGGGA TTCCTCCATA ATTTCCAGGA GACAACCTAT 
CTGCGTCCTA CCTTCTGTGA TAACTGCGCA GGATTTTTGT GGGGAGTGAT CAAGCAAGGA 
TACCGATGCA AAGATTGTGG AATGAATTGC CATAAACAGT GTAAAGAACT GGTGGTATTT 
GAATGTAAGA AGAGGTCGAA ACTCTCGGTT GGAGAGAACA GTTCCATGTT TGACTCTGGA 
CAGTTAGAAG TTATTCCAGC GGGAGGCAAA GGGCAAACAA ATGACTGCTT GGGAGCAGAG 
GAAGGACCCT ATTCCTATCC TAATGGTGAT GGTGACATCC ACACAGAGGT CAGTAAGGAT 
CGGACCATTA TGCTTATGGG GAGCTCAGCA CAGAAGATCT CAGTACGACT GCAGCCAGCT 
GTGAAACACA GAGCAACGCA GACAGAGAAT GAGCCACAAA GCCTGTGCCT ACAAGTTCCG 
TCTCCACAGC GCAGCAGGAC CCCAGGCCTA ACCTCCCATC TCCCTATAAG CCCCATGCCT 
TCCCCCTGTC CCAGTCCAGT TCCCACACGA AAGAAGGCTT ATGCCAAATG GGAAAACAAG 
GATTCCATCC GTAAGGCAAG AGCAGAATTG AGGGGAGGCA AAGCTGGCAT ACAGGAGTTG 
GAGAAGGAAA AGGCCTTACT AAAGGAGGAA AACACAACTC TAAAGATCCA GCTGAAGGAT 
GCTCAGAGAC GTGTTGAGAC TCTGCGGGCA GAGTTAAGGA AATATGTATT GGACAGCGAC 
GTGCATCAGA CGGGATCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012823301.1
CDS	246..2486
Translation	MMPLGKLSKGASLDDLIQMCIQAFDLDGNMGQNSELLQIMLTMHGFLLPSTELLMKLRTLYQDALQNRSFSFCLRICYFIRYWVTELWVMFKMDAKLTQAMEEFQELVRSKGEELHWRLIDTAQINSRDWSRKLTQRIKPNCSKKRKVSLLFDHLEPQELAEHLTYLEFKAFRRISFSDYQNYIVSGCVKENPTMERSIALCNGISQWVQLMVLSRPTPQLRAEVLTKFIHVAQKLHQLQNFNTLMAVIGGLCHSSISRLKDTSAHVSHDVNKVLNEMTELLSSCRNYDNYRRVYNECTNFKIPILGVHLKDLIALHEAMPDFLEDSKINVPKLHSLYNHINELIQLQNIAPPLEANMDLVHLLTLSLDLYYTEDEMYELSYAREPRNHRAPPVTPSKPPVVADWASGVSPKPDPKTISKHVQRMVDSVFKNYDLDQDGYISQEEFEKIAASFPFSFCVMDKDREGLISRQEITAYFMRASSICSKLGLGFLHNFQETTYLRPTFCDNCAGFLWGVIKQGYRCKDCGMNCHKQCKELVVFECKKRSKLSVGENSSMFDSGQLEVIPAGGKGQTNDCLGAEEGPYSYPNGDGDIHTEVSKDRTIMLMGSSAQKISVRLQPAVKHRATQTENEPQSLCLQVPSPQRSRTPGLTSHLPISPMPSPCPSPVPTRKKAYAKWENKDSIRKARAELRGGKAGIQELEKEKALLKEENTTLKIQLKDAQRRVETLRAELRKYVLDSDVHQTGS

Target ORF information:

RefSeq Version	XM_012967847.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis RAS guanyl releasing protein 1 (rasgrp1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967847.3

ORF Insert Sequence:

ATGATGCCTC TTGGAAAACT CTCTAAAGGA GCCAGCTTGG ATGACCTGAT ACAGATGTGC 
ATTCAGGCTT TCGATTTAGA TGGCAATATG GGGCAGAACA GCGAGCTGCT GCAGATTATG 
CTGACTATGC ATGGCTTCCT GCTTCCCTCC ACAGAATTGC TCATGAAGCT CAGGACACTA 
TATCAGGATG CCCTGCAGAA CAGATCTTTT TCATTCTGCC TCAGGATCTG CTACTTTATA 
AGATACTGGG TCACAGAACT CTGGGTCATG TTCAAAATGG ATGCAAAACT CACTCAGGCA 
ATGGAAGAGT TCCAGGAGCT GGTGAGATCG AAAGGGGAGG AGTTACACTG GCGCTTGATT 
GACACTGCTC AAATAAACTC AAGGGACTGG TCCAGAAAAC TGACACAAAG AATCAAACCC 
AACTGCAGCA AGAAGAGGAA AGTGTCTCTG CTGTTTGACC ACTTGGAGCC ACAGGAACTT 
GCTGAACACT TGACCTACCT GGAGTTTAAA GCCTTTCGAA GAATATCATT TTCAGATTAT 
CAGAACTACA TTGTCAGTGG CTGTGTGAAG GAAAATCCAA CCATGGAACG ATCCATTGCG 
CTGTGCAATG GAATCTCGCA ATGGGTCCAG CTTATGGTTC TGAGCCGTCC AACTCCCCAG 
CTCCGGGCTG AGGTGCTCAC AAAGTTCATC CATGTAGCTC AGAAACTGCA TCAGCTGCAG 
AATTTCAACA CCTTAATGGC TGTCATTGGG GGCCTTTGTC ATAGCTCCAT CTCCAGGCTC 
AAGGATACGA GTGCACATGT ATCCCATGAT GTCAACAAGG TCCTGAATGA AATGACAGAG 
CTGCTGTCTT CTTGCAGGAA TTATGACAAT TACCGACGAG TGTACAATGA GTGCACCAAC 
TTTAAGATTC CTATCCTGGG AGTCCACCTG AAGGACCTGA TTGCTCTACA TGAGGCCATG 
CCAGACTTTC TAGAGGACAG CAAAATTAAT GTGCCAAAGC TTCACTCTTT ATATAATCAC 
ATCAATGAGC TCATCCAGTT GCAGAACATT GCTCCTCCAT TGGAAGCAAA CATGGACCTT 
GTACATCTAC TCACGCTCTC CCTTGATTTA TATTATACTG AAGATGAAAT GTATGAGCTT 
TCTTACGCAC GGGAGCCAAG AAATCACCGG GCACCGCCAG TCACACCCTC CAAACCTCCT 
GTGGTTGCAG ACTGGGCATC AGGAGTCTCT CCAAAGCCAG ACCCTAAAAC TATCAGCAAG 
CATGTGCAGA GGATGGTGGA TTCTGTATTT AAGAATTATG ACCTGGATCA AGATGGATAT 
ATATCCCAAG AAGAATTTGA GAAGATAGCA GCCAGCTTCC CTTTCTCTTT CTGCGTCATG 
GATAAAGACA GGGAAGGGTT AATCAGCAGG CAAGAGATAA CTGCTTACTT CATGCGAGCC 
AGCTCTATTT GCTCAAAGCT TGGCCTGGGA TTCCTCCATA ATTTCCAGGA GACAACCTAT 
CTGCGTCCTA CCTTCTGTGA TAACTGCGCA GGATTTTTGT GGGGAGTGAT CAAGCAAGGA 
TACCGATGCA AAGATTGTGG AATGAATTGC CATAAACAGT GTAAAGAACT GGTGGTATTT 
GAATGTAAGA AGAGGTCGAA ACTCTCGGTT GGAGAGAACA GTTCCATGTT TGACTCTGGA 
CAGTTAGAAG TTATTCCAGC GGGAGGCAAA GGGCAAACAA ATGACTGCTT GGGAGCAGAG 
GAAGGACCCT ATTCCTATCC TAATGGTGAT GGTGACATCC ACACAGAGGT CAGTAAGGAT 
CGGACCATTA TGCTTATGGG GAGCTCAGCA CAGAAGATCT CAGTACGACT GCAGCCAGCT 
GTGAAACACA GAGCAACGCA GACAGAGAAT GAGCCACAAA GCCTGTGCCT ACAAGTTCCG 
TCTCCACAGC GCAGCAGGAC CCCAGGCCTA ACCTCCCATC TCCCTATAAG CCCCATGCCT 
TCCCCCTGTC CCAGTCCAGT TCCCACACGA AAGAAGGCTT ATGCCAAATG GGAAAACAAG 
GATTCCATCC GTAAGGCAAG AGCAGAATTG AGGGGAGGCA AAGCTGGCAT ACAGGAGTTG 
GAGAAGGAAA AGGCCTTACT AAAGGAGGAA AACACAACTC TAAAGATCCA GCTGAAGGAT 
GCTCAGAGAC GTGTTGAGAC TCTGCGGGCA GAGTTAAGGA AATATGTATT GGACAGCGAC 
GTGCATCAGA CGGGATCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rasgrp1 ( XM_018096099.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951588.1 Xenopus tropicalis RAS guanyl releasing protein 1 (calcium and DAG-regulated) (rasgrp1), transcript variant X1, mRNA.

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CloneID	OXa45935
Clone ID Related Accession (Same CDS sequence)	XM_018096099.1
Accession Version	XM_018096099.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	RAS guanyl-releasing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATACA GACTGGGGGC TGATACTAAA CATCACTCCG ACTACTGCAG CATCTTCGTC 
ATGGGGACTG TCGGCAAGAA GAAGGACCGA CCCACTCATG GCTGTTCCAC CATCCCCAAA 
CTCGCTCTGG AGCTGAAGCA GATCATCCAC TCTACCACCC ATCCCAAAGT GCCCCCTGTC 
ACTCCCTTAC GGGTGATGAT GCCTCTTGGA AAACTCTCTA AAGGAGCCAG CTTGGATGAC 
CTGATACAGA TGTGCATTCA GGCTTTCGAT TTAGATGGCA ATATGGGGCA GAACAGCGAG 
CTGCTGCAGA TTATGCTGAC TATGCATGGC TTCCTGCTTC CCTCCACAGA ATTGCTCATG 
AAGCTCAGGA CACTATATCA GGATGCCCTG CAGAACAGAT CTTTTTCATT CTGCCTCAGG 
ATCTGCTACT TTATAAGATA CTGGGTCACA GAACTCTGGG TCATGTTCAA AATGGATGCA 
AAACTCACTC AGGCAATGGA AGAGTTCCAG GAGCTGGTGA GATCGAAAGG GGAGGAGTTA 
CACTGGCGCT TGATTGACAC TGCTCAAATA AACTCAAGGG ACTGGTCCAG AAAACTGACA 
CAAAGAATCA AACCCAACTG CAGCAAGAAG AGGAAAGTGT CTCTGCTGTT TGACCACTTG 
GAGCCACAGG AACTTGCTGA ACACTTGACC TACCTGGAGT TTAAAGCCTT TCGAAGAATA 
TCATTTTCAG ATTATCAGAA CTACATTGTC AGTGGCTGTG TGAAGGAAAA TCCAACCATG 
GAACGATCCA TTGCGCTGTG CAATGGAATC TCGCAATGGG TCCAGCTTAT GGTTCTGAGC 
CGTCCAACTC CCCAGCTCCG GGCTGAGGTG CTCACAAAGT TCATCCATGT AGCTCAGAAA 
CTGCATCAGC TGCAGAATTT CAACACCTTA ATGGCTGTCA TTGGGGGCCT TTGTCATAGC 
TCCATCTCCA GGCTCAAGGA TACGAGTGCA CATGTATCCC ATGATGTCAA CAAGGTCCTG 
AATGAAATGA CAGAGCTGCT GTCTTCTTGC AGGAATTATG ACAATTACCG ACGAGTGTAC 
AATGAGTGCA CCAACTTTAA GATTCCTATC CTGGGAGTCC ACCTGAAGGA CCTGATTGCT 
CTACATGAGG CCATGCCAGA CTTTCTAGAG GACAGCAAAA TTAATGTGCC AAAGCTTCAC 
TCTTTATATA ATCACATCAA TGAGCTCATC CAGTTGCAGA ACATTGCTCC TCCATTGGAA 
GCAAACATGG ACCTTGTACA TCTACTCACG CCAGTCACAC CCTCCAAACC TCCTGTGGTT 
GCAGACTGGG CATCAGGAGT CTCTCCAAAG CCAGACCCTA AAACTATCAG CAAGCATGTG 
CAGAGGATGG TGGATTCTGT ATTTAAGAAT TATGACCTGG ATCAAGATGG ATATATATCC 
CAAGAAGAAT TTGAGAAGAT AGCAGCCAGC TTCCCTTTCT CTTTCTGCGT CATGGATAAA 
GACAGGGAAG GGTTAATCAG CAGGCAAGAG ATAACTGCTT ACTTCATGCG AGCCAGCTCT 
ATTTGCTCAA AGCTTGGCCT GGGATTCCTC CATAATTTCC AGGAGACAAC CTATCTGCGT 
CCTACCTTCT GTGATAACTG CGCAGGATTT TTGTGGGGAG TGATCAAGCA AGGATACCGA 
TGCAAAGATT GTGGAATGAA TTGCCATAAA CAGTGTAAAG AACTGGTGGT ATTTGAATGT 
AAGAAGAGGT CGAAACTCTC GGTTGGAGAG AACAGTTCCA TGTTTGACTC TGGACAGTTA 
GAAGTTATTC CAGCGGGAGG CAAAGGGCAA ACAAATGACT GCTTGGGAGC AGAGGAAGGA 
CCCTATTCCT ATCCTAATGG TGATGGTGAC ATCCACACAG AGGTCAGTAA GGATCGGACC 
ATTATGCTTA TGGGGAGCTC AGCACAGAAG ATCTCAGTAC GACTGCAGCC AGCTGTGAAA 
CACAGAGCAA CGCAGACAGA GAATGAGCCA CAAAGCCTGT GCCTACAAGT TCCGTCTCCA 
CAGCGCAGCA GGACCCCAGG CCTAACCTCC CATCTCCCTA TAAGCCCCAT GCCTTCCCCC 
TGTCCCAGTC CAGTTCCCAC ACGAAAGAAG GCTTATGCCA AATGGGAAAA CAAGGATTCC 
ATCCGTAAGG CAAGAGCAGA ATTGAGGGGA GGCAAAGCTG GCATACAGGA GTTGGAGAAG 
GAAAAGGCCT TACTAAAGGA GGAAAACACA ACTCTAAAGA TCCAGCTGAA GGATGCTCAG 
AGACGTGTTG AGACTCTGCG GGCAGAGTTA AGGAAATATG TATTGGACAG CGACGTGCAT 
CAGACGGGAT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951588.1
CDS	277..2631
Translation	MGYRLGADTKHHSDYCSIFVMGTVGKKKDRPTHGCSTIPKLALELKQIIHSTTHPKVPPVTPLRVMMPLGKLSKGASLDDLIQMCIQAFDLDGNMGQNSELLQIMLTMHGFLLPSTELLMKLRTLYQDALQNRSFSFCLRICYFIRYWVTELWVMFKMDAKLTQAMEEFQELVRSKGEELHWRLIDTAQINSRDWSRKLTQRIKPNCSKKRKVSLLFDHLEPQELAEHLTYLEFKAFRRISFSDYQNYIVSGCVKENPTMERSIALCNGISQWVQLMVLSRPTPQLRAEVLTKFIHVAQKLHQLQNFNTLMAVIGGLCHSSISRLKDTSAHVSHDVNKVLNEMTELLSSCRNYDNYRRVYNECTNFKIPILGVHLKDLIALHEAMPDFLEDSKINVPKLHSLYNHINELIQLQNIAPPLEANMDLVHLLTPVTPSKPPVVADWASGVSPKPDPKTISKHVQRMVDSVFKNYDLDQDGYISQEEFEKIAASFPFSFCVMDKDREGLISRQEITAYFMRASSICSKLGLGFLHNFQETTYLRPTFCDNCAGFLWGVIKQGYRCKDCGMNCHKQCKELVVFECKKRSKLSVGENSSMFDSGQLEVIPAGGKGQTNDCLGAEEGPYSYPNGDGDIHTEVSKDRTIMLMGSSAQKISVRLQPAVKHRATQTENEPQSLCLQVPSPQRSRTPGLTSHLPISPMPSPCPSPVPTRKKAYAKWENKDSIRKARAELRGGKAGIQELEKEKALLKEENTTLKIQLKDAQRRVETLRAELRKYVLDSDVHQTGS

Target ORF information:

RefSeq Version	XM_018096099.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis RAS guanyl releasing protein 1 (calcium and DAG-regulated) (rasgrp1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096099.1

ORF Insert Sequence:

ATGGGATACA GACTGGGGGC TGATACTAAA CATCACTCCG ACTACTGCAG CATCTTCGTC 
ATGGGGACTG TCGGCAAGAA GAAGGACCGA CCCACTCATG GCTGTTCCAC CATCCCCAAA 
CTCGCTCTGG AGCTGAAGCA GATCATCCAC TCTACCACCC ATCCCAAAGT GCCCCCTGTC 
ACTCCCTTAC GGGTGATGAT GCCTCTTGGA AAACTCTCTA AAGGAGCCAG CTTGGATGAC 
CTGATACAGA TGTGCATTCA GGCTTTCGAT TTAGATGGCA ATATGGGGCA GAACAGCGAG 
CTGCTGCAGA TTATGCTGAC TATGCATGGC TTCCTGCTTC CCTCCACAGA ATTGCTCATG 
AAGCTCAGGA CACTATATCA GGATGCCCTG CAGAACAGAT CTTTTTCATT CTGCCTCAGG 
ATCTGCTACT TTATAAGATA CTGGGTCACA GAACTCTGGG TCATGTTCAA AATGGATGCA 
AAACTCACTC AGGCAATGGA AGAGTTCCAG GAGCTGGTGA GATCGAAAGG GGAGGAGTTA 
CACTGGCGCT TGATTGACAC TGCTCAAATA AACTCAAGGG ACTGGTCCAG AAAACTGACA 
CAAAGAATCA AACCCAACTG CAGCAAGAAG AGGAAAGTGT CTCTGCTGTT TGACCACTTG 
GAGCCACAGG AACTTGCTGA ACACTTGACC TACCTGGAGT TTAAAGCCTT TCGAAGAATA 
TCATTTTCAG ATTATCAGAA CTACATTGTC AGTGGCTGTG TGAAGGAAAA TCCAACCATG 
GAACGATCCA TTGCGCTGTG CAATGGAATC TCGCAATGGG TCCAGCTTAT GGTTCTGAGC 
CGTCCAACTC CCCAGCTCCG GGCTGAGGTG CTCACAAAGT TCATCCATGT AGCTCAGAAA 
CTGCATCAGC TGCAGAATTT CAACACCTTA ATGGCTGTCA TTGGGGGCCT TTGTCATAGC 
TCCATCTCCA GGCTCAAGGA TACGAGTGCA CATGTATCCC ATGATGTCAA CAAGGTCCTG 
AATGAAATGA CAGAGCTGCT GTCTTCTTGC AGGAATTATG ACAATTACCG ACGAGTGTAC 
AATGAGTGCA CCAACTTTAA GATTCCTATC CTGGGAGTCC ACCTGAAGGA CCTGATTGCT 
CTACATGAGG CCATGCCAGA CTTTCTAGAG GACAGCAAAA TTAATGTGCC AAAGCTTCAC 
TCTTTATATA ATCACATCAA TGAGCTCATC CAGTTGCAGA ACATTGCTCC TCCATTGGAA 
GCAAACATGG ACCTTGTACA TCTACTCACG CCAGTCACAC CCTCCAAACC TCCTGTGGTT 
GCAGACTGGG CATCAGGAGT CTCTCCAAAG CCAGACCCTA AAACTATCAG CAAGCATGTG 
CAGAGGATGG TGGATTCTGT ATTTAAGAAT TATGACCTGG ATCAAGATGG ATATATATCC 
CAAGAAGAAT TTGAGAAGAT AGCAGCCAGC TTCCCTTTCT CTTTCTGCGT CATGGATAAA 
GACAGGGAAG GGTTAATCAG CAGGCAAGAG ATAACTGCTT ACTTCATGCG AGCCAGCTCT 
ATTTGCTCAA AGCTTGGCCT GGGATTCCTC CATAATTTCC AGGAGACAAC CTATCTGCGT 
CCTACCTTCT GTGATAACTG CGCAGGATTT TTGTGGGGAG TGATCAAGCA AGGATACCGA 
TGCAAAGATT GTGGAATGAA TTGCCATAAA CAGTGTAAAG AACTGGTGGT ATTTGAATGT 
AAGAAGAGGT CGAAACTCTC GGTTGGAGAG AACAGTTCCA TGTTTGACTC TGGACAGTTA 
GAAGTTATTC CAGCGGGAGG CAAAGGGCAA ACAAATGACT GCTTGGGAGC AGAGGAAGGA 
CCCTATTCCT ATCCTAATGG TGATGGTGAC ATCCACACAG AGGTCAGTAA GGATCGGACC 
ATTATGCTTA TGGGGAGCTC AGCACAGAAG ATCTCAGTAC GACTGCAGCC AGCTGTGAAA 
CACAGAGCAA CGCAGACAGA GAATGAGCCA CAAAGCCTGT GCCTACAAGT TCCGTCTCCA 
CAGCGCAGCA GGACCCCAGG CCTAACCTCC CATCTCCCTA TAAGCCCCAT GCCTTCCCCC 
TGTCCCAGTC CAGTTCCCAC ACGAAAGAAG GCTTATGCCA AATGGGAAAA CAAGGATTCC 
ATCCGTAAGG CAAGAGCAGA ATTGAGGGGA GGCAAAGCTG GCATACAGGA GTTGGAGAAG 
GAAAAGGCCT TACTAAAGGA GGAAAACACA ACTCTAAAGA TCCAGCTGAA GGATGCTCAG 
AGACGTGTTG AGACTCTGCG GGCAGAGTTA AGGAAATATG TATTGGACAG CGACGTGCAT 
CAGACGGGAT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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rasgrp1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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