gli3 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	gli3
Entrez Gene ID	100101789
Full Name	GLI family zinc finger 3
Synonyms	XGli-3,Xgli3
General protein information	Preferred Names GLI family zinc finger 3 Names GLI family zinc finger 3 transcriptional activator GLI3 GLI-Kruppel family member GLI3 (Greig cephalopolysyndactyly syndrome)
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004915350.3	XP_004915407.1	transcriptional activator GLI3 isoform X1
XM_004915350.4	XP_004915407.1	transcriptional activator GLI3 isoform X1
XM_012965492.2	XP_012820946.1	transcriptional activator GLI3 isoform X2
XM_012965492.3	XP_012820946.1	transcriptional activator GLI3 isoform X2
XM_004915351.4	XP_004915408.1	transcriptional activator GLI3 isoform X1
XM_004915351.3	XP_004915408.1	transcriptional activator GLI3 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	GLI3	NP_000159.3
Rattus norvegicus (Norway rat)	Gli3	NP_536330.1
Danio rerio (zebrafish)	gli3	NP_991291.1
Pan troglodytes (chimpanzee)	GLI3	NP_001029362.1
Canis lupus familiaris (dog)	GLI3	XP_005630984.1
Bos taurus (cattle)	LOC785371	XP_005205717.1
Gallus gallus (chicken)	GLI3	NP_001258832.1
	GLI3	XP_001098108.1
Mus musculus (house mouse)	Gli3	NP_032156.2
Xenopus tropicalis (tropical clawed frog)	gli3	XP_004915408.1

Gene Ontology

The following gli3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gli3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa11836	XM_004915350.4 Latest version!	Xenopus tropicalis GLI family zinc finger 3 (gli3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa11836	XM_004915351.4 Latest version!	Xenopus tropicalis GLI family zinc finger 3 (gli3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa30279	XM_012965492.3 Latest version!	Xenopus tropicalis GLI family zinc finger 3 (gli3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OXa11836	XM_004915350.3	Xenopus tropicalis GLI family zinc finger 3 (gli3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa30279	XM_012965492.2	Xenopus tropicalis GLI family zinc finger 3 (gli3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa11836	XM_004915351.3	Xenopus tropicalis GLI family zinc finger 3 (gli3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gli3 ( XM_004915350.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915407.1 Xenopus tropicalis GLI family zinc finger 3 (gli3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa11836
Clone ID Related Accession (Same CDS sequence)	XM_004915350.3 , XM_004915350.4 , XM_004915351.4 , XM_004915351.3
Accession Version	XM_004915350.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transcriptional activator GLI3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004915350.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCCC AGTCCCGTAG CACTACAGCA AGTGAAAAGA AAAAGGTTGA GAACTCCATA 
ATCAAAGGTC ACACTCGAAC AGAAGTCAGC GAGAAAGCTG TTGCCTCCAG CACAACTTCC 
AATGAGGATG AAAGTCCTGG ACAGACATAT CACAGAGAAA GAAGAAACGC CATCACCATG 
CAGCCTCATG GAGGCCAAGG TCTTGGTAAA ATCAATGAGG AACCCTCTAC GTCAAGTGAG 
GAAAGGGCCT CATTAATAAA AAAAGAACTT CATGGATCTA TAACACATCT GCCTGAACCC 
TCAGTTCCCT ACCGTGGCAC TGTCTTTGCC ATGGACCCTA GGAATGGTTA TATTGATCCA 
CATTACCATC CGCCTCATCT CTTCCCATCT TTTCATCCTC CTGTGCCAAT TGATGCAAGA 
CATCATGAGG GAAGATACCA TTATGAGCCA ACTCCCATTC CGCCACTGCA TGTGCCTTCT 
GCATTAGCAA GCAGCCCGAC ATACCCAGAT CTTCCATTTA TCCGGATATC TCCACACAGA 
AACCCAGCCA GTGCATCTGA TTCCCCATTC AGCCCTCCTC ATCCTTACAT CAGTCCTTAC 
ATGGACTATA TCAGATCTCT CCACAGCAGC CCTTCACTGT CTATGATATC AGCTGCCCGT 
GGACTCAGCC CTACTGATGT CCCACATGCT GGTGTTAGCC CTGCTGAATA TTACCATCAA 
ATGGCACTGC TAACTGGACA GAGAAGCCCA TATGCTGATA TTATTCCATC CCCTGCAACT 
GCTGGTGCTG GAGCTAATGC CCTCCATATG GAATATCTTC ATGCCATGGA GAGCTCACGG 
TTTTCAAGTC CTAGAGTGCC ATTACGACCA AGTCGCAAGA GAACACTGTC CATATCACCT 
CTTTCTGATC ATAGCTTTGA TCTCCAGACA ATGATTCGCA ACTCTCCAAA TTCTCTTGTT 
ACTATACTTA ACAACTCCCG CAGCAGCTCA TCTGCTAGTG GCTCTTATGG GCATCTAGCA 
GCCAGTGCCA TCAGTCCAGC ATTGAGTTTT ACATACCAGC CTACACCAGT GTCTCTTCAG 
CAAATGCATC AACAGCTTAT GAGTCGACAG CACAGCATAG GCTCAGCTTT TGGACACAGC 
CCTCCACTTC TTCACCCAGC ACCAACATTT CCTTCTCAAA GGACCATTCC CGGCATCCCC 
AGTGTTTTGA ATCCAGTGCA GGTCAGCATT GGGCCATCAG AGGCTGCACA GCAAAACAAG 
CCCACCAGTG AGTCAGCAGT CAGCAGCACA GGTGACCCCC TGCACAACAA GCGTTCTAAG 
GTAAAACCTG AAGAAGATCT TCCAAGCCCT GGAGCAGTGT GTATTCAGGA TCAGCCAGAT 
GGAATGACTC TGGTGAAAGA GGAGGGGGAC AAAGATGAAA GTAAGCAAGA GCCTGAAGTG 
GTCTACGAGA CAAACTGCCA TTGGGAAGGC TGCTCGCGAG AGTTTGACAC ACAGGAACAG 
CTAGTGCATC ATATCAACAA TGACCACATA CATGGAGAGA AAAAGGAGTT TGTGTGCCGA 
TGGCTAGATT GTTCAAGAGA ACAAAAGCCG TTTAAGGCTC AGTACATGCT GGTGGTCCAC 
ATGAGAAGAC ATACAGGAGA AAAGCCACAC AAATGCACTT TTGAAGGTTG TTCAAAAGCC 
TACTCCAGAC TAGAAAACTT GAAAACTCAC CTGAGATCTC ACACTGGAGA GAAACCATAT 
GTCTGTGAGC ATGAGGGCTG CAACAAGGCC TTTTCAAATG CATCTGATAG AGCCAAGCAC 
CAAAACCGCA CTCATTCAAA TGAGAAACCT TATGTTTGCA AGATCCCAGG GTGTACAAAA 
CGTTACACTG ACCCTAGCTC ACTTAGAAAG CATGTAAAGA CAGTGCATGG TCCAGAGGCT 
CATGTAACTA AGAAGCAGAG AGGGGACATA CACCCAAGGC CTCCACCACC ACGAGAACCA 
GGAAGCCATT CGCAGTCAAG ATCACCAGGC CAACATATCC AGGGTACACA TGGAGAGCAC 
AAGGACCTCA GTAACACTAC CTCAAAGCAT GAAGAATGCC TGCAAGTAAA ATCAGTCAAG 
ACTGAAAAGC CAATGACATC TCAGCCAAGC CCTGGTGGTA AGTCTTCATG CAGCAGCGAG 
CAGTCCCCCG TCAGCAACTA TACCAACAGT GGGATCGAGC TTACTCTAAA CAGTGGAGGC 
AGCATTGGAG ACTTCAGTGC CCTAGATGAA ACCCCAATCA TGGACTCTAC CATTTCCACC 
GCAACCACAG GACTTGGTTT ACACGGAAGG AGGATTATGA CAGGAACCAA ATGGATGGAG 
CAAATTAAAG TTGAAAAAAT TAAACAAGTT AATGGTATTC TTCCAAGACT GAACCCAGTT 
CCACCTCATA GAGCACCAAA TTTACCACCA ATCACTGGAA ATGGTGTGCA GCTGAACAGC 
AACATCAGTT TGGGGGGATC AGCACCTGTT CTTCCAAACA AAAATGAGCT TTCTAGCATG 
GACATTACAG TCTTGAATAT GCTTAACAGA AGAGATAGCA ATACTAGCAC AATAAGTTCA 
GCATATATGA GCAGCCGCAG GTCCTCTGGA ATTTCACCTT GTTTTTCCAG TCGAAGATCC 
AGTGAGGCAT CTCAGTTTGA GGGACGGGTT CAGAATATAA GTGTTGCAGA CTCCTATGAT 
CCAATTTCTA CTGATGCCTC AAGACGATCA AGTGAAACAA GCCAGTGTGA TGGGCTGCCA 
AGCCTCCTAA GCCTTACTCC AGCACAGCAA TACAGGCTGA AAGCTAAATA TGCTGCTGCT 
ACAGGTGGGC CCCCACCAAC ACCACTGCCG AACATGGAAA GAATGAGTCT AAAAACCAGA 
ATAGCTCTCC TGGGTGACTC AAGAGATTGT AGATTGTCAC CTCTTCCTTC AGTCAATGTC 
CCACGAAGAT GTAGTGATGG TGGAGTAGGC TCTTATGGAA GAAGGCATTT GTTGTCAAAT 
GAACTTCAAG GTAATGGAAT GCGTAGAGCA AGTGATCCTG TAAAAATGGT CTCTGATAAC 
TTTTCTAATG CTAGAGTTCC AAGGTTCAAC AGTCTTAACA ACATCACCCC TCCTGATGCC 
CCGCCACTTA TGGAAAGGCG CAATTTAAGT CTTCAGAACT ATACCCGTAA TGTGTATTCA 
CCATGCCCTC CTAGTATAAG TGAAAATGTT GCCCTGGAAG CAATGATAAT GGAAGCAGAA 
GGCAATTTTG ATGATGAGGA TTTACTGCCA GATGACATGG TTCAGTATCT TAACTCTCGG 
AACCAAAGAC TTTATGACAT TTTTGAAACA GATAAATTAC AGAATAATGC TGGAGAAAGA 
AATGGAAACA ACTTTGAACC ATCTCATGTA ACCAGTAATA TAATAAATGA ACAGTTCCTC 
AATTCCGAAC AAACAGATAC CATAGAAAAT AAAAATGACC TCCCAATTCA GTGGAATGAA 
GTAAGCTCTG GCAGTGCAGA ATATTCTCCA TCGAGATTAA AGTATGGGCA GCGTTTAACC 
ACACAGCAGA ATCGACCTTT TGGATTGTAT AATAACATGA TGGTCCAGCA GCAAAATGTA 
CCAAAGAACG GGTTGCCCCA GCAAAGGAGC TATCAACATC ATACACAAAA CAACCCACAG 
GTGCCCCAGC AAAATCTGAA CCTTTACAAT AACAGCAGTG TGTGGAGTGG TCAGTCAGTC 
AGGGGCAATC AGTGCATTGA CATAGCAAGA TCATCACTGG GAACGTCTGC AACAGGAAGT 
CTTTGCAATA CAACTCGAGG ACAAAAATTT ACAGCCAATG GTTTGCAAAT GAATATTGGT 
CAACAAAACT ATGGACCATC CACACATTAT AGTGTGCAAT CAGCTAGCAG AACTGACCCA 
GTCCAGAATG AAAACATCAT GAATCAGGAA TTTATGCACC AGATGACAGC CGAACACCTA 
ATTTATGGTA TGCATTTCCT GGGGGTAACT CGATCTAATC AGACAACATC AGGGCAAAAT 
GGAAACACAA TTGATGCAAC ACAAAGTTTC CTGTCAACTA CTCAAAATGC TGGAGAGCAG 
CAGCCATCCC TGGCAAAGAA TATCCAGTCT TTTGTAAACT CTAGTGGAAA TAGCAGGCAG 
AATATACATA GAAACAACCT GTCATATCTG CAAGAGCAAA TATGTGACAC CAACCAACAA 
GTTTGCAGAG TGAATGGTAT TAAAATGGAA ATGCAGAATC ACCCACAACA GCACTGTGCG 
AATGCACAAA ACTTCTCCGG TCAGTTGTAT GACCAAACAG CAGCATATCC TCAGCAAAGT 
ATCAAGTCAG GTTCTGAGCC TGGTATGGAA GCAAACTGTC TTTTACAAGA AAGTAATAAT 
ACAAACTCAT CCCAGCTTCT TTCGCCTGGG GCAAACCAAG TGACTAGCAC AGTCAATAAC 
ATTGACAGCA GTTTGGATGA GGTGCAAATC GATTTTGATG CTATAATTGA TGATGGGGAT 
CATGCCAGCC TCATTTCTGG AGCTCTAAGT CCAAGTATCA TCCAGAATCT CTCCCGTAAT 
TCTTCACGCC TTACCACTCC TCGAACATCT CTGACATTCC CAGCCATACC TGTTAGTACC 
AGTAACATGG CTATTGGGGA CATGAGCTCT TTGCTGACCT CACTTGCAGA AGAAAGCAAG 
TTTCTTGCTA TTATGCAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915407.1
CDS	404..5104
Translation	MEAQSRSTTASEKKKVENSIIKGHTRTEVSEKAVASSTTSNEDESPGQTYHRERRNAITMQPHGGQGLGKINEEPSTSSEERASLIKKELHGSITHLPEPSVPYRGTVFAMDPRNGYIDPHYHPPHLFPSFHPPVPIDARHHEGRYHYEPTPIPPLHVPSALASSPTYPDLPFIRISPHRNPASASDSPFSPPHPYISPYMDYIRSLHSSPSLSMISAARGLSPTDVPHAGVSPAEYYHQMALLTGQRSPYADIIPSPATAGAGANALHMEYLHAMESSRFSSPRVPLRPSRKRTLSISPLSDHSFDLQTMIRNSPNSLVTILNNSRSSSSASGSYGHLAASAISPALSFTYQPTPVSLQQMHQQLMSRQHSIGSAFGHSPPLLHPAPTFPSQRTIPGIPSVLNPVQVSIGPSEAAQQNKPTSESAVSSTGDPLHNKRSKVKPEEDLPSPGAVCIQDQPDGMTLVKEEGDKDESKQEPEVVYETNCHWEGCSREFDTQEQLVHHINNDHIHGEKKEFVCRWLDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCSKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDIHPRPPPPREPGSHSQSRSPGQHIQGTHGEHKDLSNTTSKHEECLQVKSVKTEKPMTSQPSPGGKSSCSSEQSPVSNYTNSGIELTLNSGGSIGDFSALDETPIMDSTISTATTGLGLHGRRIMTGTKWMEQIKVEKIKQVNGILPRLNPVPPHRAPNLPPITGNGVQLNSNISLGGSAPVLPNKNELSSMDITVLNMLNRRDSNTSTISSAYMSSRRSSGISPCFSSRRSSEASQFEGRVQNISVADSYDPISTDASRRSSETSQCDGLPSLLSLTPAQQYRLKAKYAAATGGPPPTPLPNMERMSLKTRIALLGDSRDCRLSPLPSVNVPRRCSDGGVGSYGRRHLLSNELQGNGMRRASDPVKMVSDNFSNARVPRFNSLNNITPPDAPPLMERRNLSLQNYTRNVYSPCPPSISENVALEAMIMEAEGNFDDEDLLPDDMVQYLNSRNQRLYDIFETDKLQNNAGERNGNNFEPSHVTSNIINEQFLNSEQTDTIENKNDLPIQWNEVSSGSAEYSPSRLKYGQRLTTQQNRPFGLYNNMMVQQQNVPKNGLPQQRSYQHHTQNNPQVPQQNLNLYNNSSVWSGQSVRGNQCIDIARSSLGTSATGSLCNTTRGQKFTANGLQMNIGQQNYGPSTHYSVQSASRTDPVQNENIMNQEFMHQMTAEHLIYGMHFLGVTRSNQTTSGQNGNTIDATQSFLSTTQNAGEQQPSLAKNIQSFVNSSGNSRQNIHRNNLSYLQEQICDTNQQVCRVNGIKMEMQNHPQQHCANAQNFSGQLYDQTAAYPQQSIKSGSEPGMEANCLLQESNNTNSSQLLSPGANQVTSTVNNIDSSLDEVQIDFDAIIDDGDHASLISGALSPSIIQNLSRNSSRLTTPRTSLTFPAIPVSTSNMAIGDMSSLLTSLAEESKFLAIMQ

Target ORF information:

RefSeq Version	XM_004915350.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis GLI family zinc finger 3 (gli3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915350.4

ORF Insert Sequence:

ATGGAAGCCC AGTCCCGTAG CACTACAGCA AGTGAAAAGA AAAAGGTTGA GAACTCCATA 
ATCAAAGGTC ACACTCGAAC AGAAGTCAGC GAGAAAGCTG TTGCCTCCAG CACAACTTCC 
AATGAGGATG AAAGTCCTGG ACAGACATAT CACAGAGAAA GAAGAAACGC CATCACCATG 
CAGCCTCATG GAGGCCAAGG TCTTGGTAAA ATCAATGAGG AACCCTCTAC GTCAAGTGAG 
GAAAGGGCCT CATTAATAAA AAAAGAACTT CATGGATCTA TAACACATCT GCCTGAACCC 
TCAGTTCCCT ACCGTGGCAC TGTCTTTGCC ATGGACCCTA GGAATGGTTA TATTGATCCA 
CATTACCATC CGCCTCATCT CTTCCCATCT TTTCATCCTC CTGTGCCAAT TGATGCAAGA 
CATCATGAGG GAAGATACCA TTATGAGCCA ACTCCCATTC CGCCACTGCA TGTGCCTTCT 
GCATTAGCAA GCAGCCCGAC ATACCCAGAT CTTCCATTTA TCCGGATATC TCCACACAGA 
AACCCAGCCA GTGCATCTGA TTCCCCATTC AGCCCTCCTC ATCCTTACAT CAGTCCTTAC 
ATGGACTATA TCAGATCTCT CCACAGCAGC CCTTCACTGT CTATGATATC AGCTGCCCGT 
GGACTCAGCC CTACTGATGT CCCACATGCT GGTGTTAGCC CTGCTGAATA TTACCATCAA 
ATGGCACTGC TAACTGGACA GAGAAGCCCA TATGCTGATA TTATTCCATC CCCTGCAACT 
GCTGGTGCTG GAGCTAATGC CCTCCATATG GAATATCTTC ATGCCATGGA GAGCTCACGG 
TTTTCAAGTC CTAGAGTGCC ATTACGACCA AGTCGCAAGA GAACACTGTC CATATCACCT 
CTTTCTGATC ATAGCTTTGA TCTCCAGACA ATGATTCGCA ACTCTCCAAA TTCTCTTGTT 
ACTATACTTA ACAACTCCCG CAGCAGCTCA TCTGCTAGTG GCTCTTATGG GCATCTAGCA 
GCCAGTGCCA TCAGTCCAGC ATTGAGTTTT ACATACCAGC CTACACCAGT GTCTCTTCAG 
CAAATGCATC AACAGCTTAT GAGTCGACAG CACAGCATAG GCTCAGCTTT TGGACACAGC 
CCTCCACTTC TTCACCCAGC ACCAACATTT CCTTCTCAAA GGACCATTCC CGGCATCCCC 
AGTGTTTTGA ATCCAGTGCA GGTCAGCATT GGGCCATCAG AGGCTGCACA GCAAAACAAG 
CCCACCAGTG AGTCAGCAGT CAGCAGCACA GGTGACCCCC TGCACAACAA GCGTTCTAAG 
GTAAAACCTG AAGAAGATCT TCCAAGCCCT GGAGCAGTGT GTATTCAGGA TCAGCCAGAT 
GGAATGACTC TGGTGAAAGA GGAGGGGGAC AAAGATGAAA GTAAGCAAGA GCCTGAAGTG 
GTCTACGAGA CAAACTGCCA TTGGGAAGGC TGCTCGCGAG AGTTTGACAC ACAGGAACAG 
CTAGTGCATC ATATCAACAA TGACCACATA CATGGAGAGA AAAAGGAGTT TGTGTGCCGA 
TGGCTAGATT GTTCAAGAGA ACAAAAGCCG TTTAAGGCTC AGTACATGCT GGTGGTCCAC 
ATGAGAAGAC ATACAGGAGA AAAGCCACAC AAATGCACTT TTGAAGGTTG TTCAAAAGCC 
TACTCCAGAC TAGAAAACTT GAAAACTCAC CTGAGATCTC ACACTGGAGA GAAACCATAT 
GTCTGTGAGC ATGAGGGCTG CAACAAGGCC TTTTCAAATG CATCTGATAG AGCCAAGCAC 
CAAAACCGCA CTCATTCAAA TGAGAAACCT TATGTTTGCA AGATCCCAGG GTGTACAAAA 
CGTTACACTG ACCCTAGCTC ACTTAGAAAG CATGTAAAGA CAGTGCATGG TCCAGAGGCT 
CATGTAACTA AGAAGCAGAG AGGGGACATA CACCCAAGGC CTCCACCACC ACGAGAACCA 
GGAAGCCATT CGCAGTCAAG ATCACCAGGC CAACATATCC AGGGTACACA TGGAGAGCAC 
AAGGACCTCA GTAACACTAC CTCAAAGCAT GAAGAATGCC TGCAAGTAAA ATCAGTCAAG 
ACTGAAAAGC CAATGACATC TCAGCCAAGC CCTGGTGGTA AGTCTTCATG CAGCAGCGAG 
CAGTCCCCCG TCAGCAACTA TACCAACAGT GGGATCGAGC TTACTCTAAA CAGTGGAGGC 
AGCATTGGAG ACTTCAGTGC CCTAGATGAA ACCCCAATCA TGGACTCTAC CATTTCCACC 
GCAACCACAG GACTTGGTTT ACACGGAAGG AGGATTATGA CAGGAACCAA ATGGATGGAG 
CAAATTAAAG TTGAAAAAAT TAAACAAGTT AATGGTATTC TTCCAAGACT GAACCCAGTT 
CCACCTCATA GAGCACCAAA TTTACCACCA ATCACTGGAA ATGGTGTGCA GCTGAACAGC 
AACATCAGTT TGGGGGGATC AGCACCTGTT CTTCCAAACA AAAATGAGCT TTCTAGCATG 
GACATTACAG TCTTGAATAT GCTTAACAGA AGAGATAGCA ATACTAGCAC AATAAGTTCA 
GCATATATGA GCAGCCGCAG GTCCTCTGGA ATTTCACCTT GTTTTTCCAG TCGAAGATCC 
AGTGAGGCAT CTCAGTTTGA GGGACGGGTT CAGAATATAA GTGTTGCAGA CTCCTATGAT 
CCAATTTCTA CTGATGCCTC AAGACGATCA AGTGAAACAA GCCAGTGTGA TGGGCTGCCA 
AGCCTCCTAA GCCTTACTCC AGCACAGCAA TACAGGCTGA AAGCTAAATA TGCTGCTGCT 
ACAGGTGGGC CCCCACCAAC ACCACTGCCG AACATGGAAA GAATGAGTCT AAAAACCAGA 
ATAGCTCTCC TGGGTGACTC AAGAGATTGT AGATTGTCAC CTCTTCCTTC AGTCAATGTC 
CCACGAAGAT GTAGTGATGG TGGAGTAGGC TCTTATGGAA GAAGGCATTT GTTGTCAAAT 
GAACTTCAAG GTAATGGAAT GCGTAGAGCA AGTGATCCTG TAAAAATGGT CTCTGATAAC 
TTTTCTAATG CTAGAGTTCC AAGGTTCAAC AGTCTTAACA ACATCACCCC TCCTGATGCC 
CCGCCACTTA TGGAAAGGCG CAATTTAAGT CTTCAGAACT ATACCCGTAA TGTGTATTCA 
CCATGCCCTC CTAGTATAAG TGAAAATGTT GCCCTGGAAG CAATGATAAT GGAAGCAGAA 
GGCAATTTTG ATGATGAGGA TTTACTGCCA GATGACATGG TTCAGTATCT TAACTCTCGG 
AACCAAAGAC TTTATGACAT TTTTGAAACA GATAAATTAC AGAATAATGC TGGAGAAAGA 
AATGGAAACA ACTTTGAACC ATCTCATGTA ACCAGTAATA TAATAAATGA ACAGTTCCTC 
AATTCCGAAC AAACAGATAC CATAGAAAAT AAAAATGACC TCCCAATTCA GTGGAATGAA 
GTAAGCTCTG GCAGTGCAGA ATATTCTCCA TCGAGATTAA AGTATGGGCA GCGTTTAACC 
ACACAGCAGA ATCGACCTTT TGGATTGTAT AATAACATGA TGGTCCAGCA GCAAAATGTA 
CCAAAGAACG GGTTGCCCCA GCAAAGGAGC TATCAACATC ATACACAAAA CAACCCACAG 
GTGCCCCAGC AAAATCTGAA CCTTTACAAT AACAGCAGTG TGTGGAGTGG TCAGTCAGTC 
AGGGGCAATC AGTGCATTGA CATAGCAAGA TCATCACTGG GAACGTCTGC AACAGGAAGT 
CTTTGCAATA CAACTCGAGG ACAAAAATTT ACAGCCAATG GTTTGCAAAT GAATATTGGT 
CAACAAAACT ATGGACCATC CACACATTAT AGTGTGCAAT CAGCTAGCAG AACTGACCCA 
GTCCAGAATG AAAACATCAT GAATCAGGAA TTTATGCACC AGATGACAGC CGAACACCTA 
ATTTATGGTA TGCATTTCCT GGGGGTAACT CGATCTAATC AGACAACATC AGGGCAAAAT 
GGAAACACAA TTGATGCAAC ACAAAGTTTC CTGTCAACTA CTCAAAATGC TGGAGAGCAG 
CAGCCATCCC TGGCAAAGAA TATCCAGTCT TTTGTAAACT CTAGTGGAAA TAGCAGGCAG 
AATATACATA GAAACAACCT GTCATATCTG CAAGAGCAAA TATGTGACAC CAACCAACAA 
GTTTGCAGAG TGAATGGTAT TAAAATGGAA ATGCAGAATC ACCCACAACA GCACTGTGCG 
AATGCACAAA ACTTCTCCGG TCAGTTGTAT GACCAAACAG CAGCATATCC TCAGCAAAGT 
ATCAAGTCAG GTTCTGAGCC TGGTATGGAA GCAAACTGTC TTTTACAAGA AAGTAATAAT 
ACAAACTCAT CCCAGCTTCT TTCGCCTGGG GCAAACCAAG TGACTAGCAC AGTCAATAAC 
ATTGACAGCA GTTTGGATGA GGTGCAAATC GATTTTGATG CTATAATTGA TGATGGGGAT 
CATGCCAGCC TCATTTCTGG AGCTCTAAGT CCAAGTATCA TCCAGAATCT CTCCCGTAAT 
TCTTCACGCC TTACCACTCC TCGAACATCT CTGACATTCC CAGCCATACC TGTTAGTACC 
AGTAACATGG CTATTGGGGA CATGAGCTCT TTGCTGACCT CACTTGCAGA AGAAAGCAAG 
TTTCTTGCTA TTATGCAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gli3 ( XM_004915351.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915408.1 Xenopus tropicalis GLI family zinc finger 3 (gli3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa11836
Clone ID Related Accession (Same CDS sequence)	XM_004915350.3 , XM_004915350.4 , XM_004915351.4 , XM_004915351.3
Accession Version	XM_004915351.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transcriptional activator GLI3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004915351.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCCC AGTCCCGTAG CACTACAGCA AGTGAAAAGA AAAAGGTTGA GAACTCCATA 
ATCAAAGGTC ACACTCGAAC AGAAGTCAGC GAGAAAGCTG TTGCCTCCAG CACAACTTCC 
AATGAGGATG AAAGTCCTGG ACAGACATAT CACAGAGAAA GAAGAAACGC CATCACCATG 
CAGCCTCATG GAGGCCAAGG TCTTGGTAAA ATCAATGAGG AACCCTCTAC GTCAAGTGAG 
GAAAGGGCCT CATTAATAAA AAAAGAACTT CATGGATCTA TAACACATCT GCCTGAACCC 
TCAGTTCCCT ACCGTGGCAC TGTCTTTGCC ATGGACCCTA GGAATGGTTA TATTGATCCA 
CATTACCATC CGCCTCATCT CTTCCCATCT TTTCATCCTC CTGTGCCAAT TGATGCAAGA 
CATCATGAGG GAAGATACCA TTATGAGCCA ACTCCCATTC CGCCACTGCA TGTGCCTTCT 
GCATTAGCAA GCAGCCCGAC ATACCCAGAT CTTCCATTTA TCCGGATATC TCCACACAGA 
AACCCAGCCA GTGCATCTGA TTCCCCATTC AGCCCTCCTC ATCCTTACAT CAGTCCTTAC 
ATGGACTATA TCAGATCTCT CCACAGCAGC CCTTCACTGT CTATGATATC AGCTGCCCGT 
GGACTCAGCC CTACTGATGT CCCACATGCT GGTGTTAGCC CTGCTGAATA TTACCATCAA 
ATGGCACTGC TAACTGGACA GAGAAGCCCA TATGCTGATA TTATTCCATC CCCTGCAACT 
GCTGGTGCTG GAGCTAATGC CCTCCATATG GAATATCTTC ATGCCATGGA GAGCTCACGG 
TTTTCAAGTC CTAGAGTGCC ATTACGACCA AGTCGCAAGA GAACACTGTC CATATCACCT 
CTTTCTGATC ATAGCTTTGA TCTCCAGACA ATGATTCGCA ACTCTCCAAA TTCTCTTGTT 
ACTATACTTA ACAACTCCCG CAGCAGCTCA TCTGCTAGTG GCTCTTATGG GCATCTAGCA 
GCCAGTGCCA TCAGTCCAGC ATTGAGTTTT ACATACCAGC CTACACCAGT GTCTCTTCAG 
CAAATGCATC AACAGCTTAT GAGTCGACAG CACAGCATAG GCTCAGCTTT TGGACACAGC 
CCTCCACTTC TTCACCCAGC ACCAACATTT CCTTCTCAAA GGACCATTCC CGGCATCCCC 
AGTGTTTTGA ATCCAGTGCA GGTCAGCATT GGGCCATCAG AGGCTGCACA GCAAAACAAG 
CCCACCAGTG AGTCAGCAGT CAGCAGCACA GGTGACCCCC TGCACAACAA GCGTTCTAAG 
GTAAAACCTG AAGAAGATCT TCCAAGCCCT GGAGCAGTGT GTATTCAGGA TCAGCCAGAT 
GGAATGACTC TGGTGAAAGA GGAGGGGGAC AAAGATGAAA GTAAGCAAGA GCCTGAAGTG 
GTCTACGAGA CAAACTGCCA TTGGGAAGGC TGCTCGCGAG AGTTTGACAC ACAGGAACAG 
CTAGTGCATC ATATCAACAA TGACCACATA CATGGAGAGA AAAAGGAGTT TGTGTGCCGA 
TGGCTAGATT GTTCAAGAGA ACAAAAGCCG TTTAAGGCTC AGTACATGCT GGTGGTCCAC 
ATGAGAAGAC ATACAGGAGA AAAGCCACAC AAATGCACTT TTGAAGGTTG TTCAAAAGCC 
TACTCCAGAC TAGAAAACTT GAAAACTCAC CTGAGATCTC ACACTGGAGA GAAACCATAT 
GTCTGTGAGC ATGAGGGCTG CAACAAGGCC TTTTCAAATG CATCTGATAG AGCCAAGCAC 
CAAAACCGCA CTCATTCAAA TGAGAAACCT TATGTTTGCA AGATCCCAGG GTGTACAAAA 
CGTTACACTG ACCCTAGCTC ACTTAGAAAG CATGTAAAGA CAGTGCATGG TCCAGAGGCT 
CATGTAACTA AGAAGCAGAG AGGGGACATA CACCCAAGGC CTCCACCACC ACGAGAACCA 
GGAAGCCATT CGCAGTCAAG ATCACCAGGC CAACATATCC AGGGTACACA TGGAGAGCAC 
AAGGACCTCA GTAACACTAC CTCAAAGCAT GAAGAATGCC TGCAAGTAAA ATCAGTCAAG 
ACTGAAAAGC CAATGACATC TCAGCCAAGC CCTGGTGGTA AGTCTTCATG CAGCAGCGAG 
CAGTCCCCCG TCAGCAACTA TACCAACAGT GGGATCGAGC TTACTCTAAA CAGTGGAGGC 
AGCATTGGAG ACTTCAGTGC CCTAGATGAA ACCCCAATCA TGGACTCTAC CATTTCCACC 
GCAACCACAG GACTTGGTTT ACACGGAAGG AGGATTATGA CAGGAACCAA ATGGATGGAG 
CAAATTAAAG TTGAAAAAAT TAAACAAGTT AATGGTATTC TTCCAAGACT GAACCCAGTT 
CCACCTCATA GAGCACCAAA TTTACCACCA ATCACTGGAA ATGGTGTGCA GCTGAACAGC 
AACATCAGTT TGGGGGGATC AGCACCTGTT CTTCCAAACA AAAATGAGCT TTCTAGCATG 
GACATTACAG TCTTGAATAT GCTTAACAGA AGAGATAGCA ATACTAGCAC AATAAGTTCA 
GCATATATGA GCAGCCGCAG GTCCTCTGGA ATTTCACCTT GTTTTTCCAG TCGAAGATCC 
AGTGAGGCAT CTCAGTTTGA GGGACGGGTT CAGAATATAA GTGTTGCAGA CTCCTATGAT 
CCAATTTCTA CTGATGCCTC AAGACGATCA AGTGAAACAA GCCAGTGTGA TGGGCTGCCA 
AGCCTCCTAA GCCTTACTCC AGCACAGCAA TACAGGCTGA AAGCTAAATA TGCTGCTGCT 
ACAGGTGGGC CCCCACCAAC ACCACTGCCG AACATGGAAA GAATGAGTCT AAAAACCAGA 
ATAGCTCTCC TGGGTGACTC AAGAGATTGT AGATTGTCAC CTCTTCCTTC AGTCAATGTC 
CCACGAAGAT GTAGTGATGG TGGAGTAGGC TCTTATGGAA GAAGGCATTT GTTGTCAAAT 
GAACTTCAAG GTAATGGAAT GCGTAGAGCA AGTGATCCTG TAAAAATGGT CTCTGATAAC 
TTTTCTAATG CTAGAGTTCC AAGGTTCAAC AGTCTTAACA ACATCACCCC TCCTGATGCC 
CCGCCACTTA TGGAAAGGCG CAATTTAAGT CTTCAGAACT ATACCCGTAA TGTGTATTCA 
CCATGCCCTC CTAGTATAAG TGAAAATGTT GCCCTGGAAG CAATGATAAT GGAAGCAGAA 
GGCAATTTTG ATGATGAGGA TTTACTGCCA GATGACATGG TTCAGTATCT TAACTCTCGG 
AACCAAAGAC TTTATGACAT TTTTGAAACA GATAAATTAC AGAATAATGC TGGAGAAAGA 
AATGGAAACA ACTTTGAACC ATCTCATGTA ACCAGTAATA TAATAAATGA ACAGTTCCTC 
AATTCCGAAC AAACAGATAC CATAGAAAAT AAAAATGACC TCCCAATTCA GTGGAATGAA 
GTAAGCTCTG GCAGTGCAGA ATATTCTCCA TCGAGATTAA AGTATGGGCA GCGTTTAACC 
ACACAGCAGA ATCGACCTTT TGGATTGTAT AATAACATGA TGGTCCAGCA GCAAAATGTA 
CCAAAGAACG GGTTGCCCCA GCAAAGGAGC TATCAACATC ATACACAAAA CAACCCACAG 
GTGCCCCAGC AAAATCTGAA CCTTTACAAT AACAGCAGTG TGTGGAGTGG TCAGTCAGTC 
AGGGGCAATC AGTGCATTGA CATAGCAAGA TCATCACTGG GAACGTCTGC AACAGGAAGT 
CTTTGCAATA CAACTCGAGG ACAAAAATTT ACAGCCAATG GTTTGCAAAT GAATATTGGT 
CAACAAAACT ATGGACCATC CACACATTAT AGTGTGCAAT CAGCTAGCAG AACTGACCCA 
GTCCAGAATG AAAACATCAT GAATCAGGAA TTTATGCACC AGATGACAGC CGAACACCTA 
ATTTATGGTA TGCATTTCCT GGGGGTAACT CGATCTAATC AGACAACATC AGGGCAAAAT 
GGAAACACAA TTGATGCAAC ACAAAGTTTC CTGTCAACTA CTCAAAATGC TGGAGAGCAG 
CAGCCATCCC TGGCAAAGAA TATCCAGTCT TTTGTAAACT CTAGTGGAAA TAGCAGGCAG 
AATATACATA GAAACAACCT GTCATATCTG CAAGAGCAAA TATGTGACAC CAACCAACAA 
GTTTGCAGAG TGAATGGTAT TAAAATGGAA ATGCAGAATC ACCCACAACA GCACTGTGCG 
AATGCACAAA ACTTCTCCGG TCAGTTGTAT GACCAAACAG CAGCATATCC TCAGCAAAGT 
ATCAAGTCAG GTTCTGAGCC TGGTATGGAA GCAAACTGTC TTTTACAAGA AAGTAATAAT 
ACAAACTCAT CCCAGCTTCT TTCGCCTGGG GCAAACCAAG TGACTAGCAC AGTCAATAAC 
ATTGACAGCA GTTTGGATGA GGTGCAAATC GATTTTGATG CTATAATTGA TGATGGGGAT 
CATGCCAGCC TCATTTCTGG AGCTCTAAGT CCAAGTATCA TCCAGAATCT CTCCCGTAAT 
TCTTCACGCC TTACCACTCC TCGAACATCT CTGACATTCC CAGCCATACC TGTTAGTACC 
AGTAACATGG CTATTGGGGA CATGAGCTCT TTGCTGACCT CACTTGCAGA AGAAAGCAAG 
TTTCTTGCTA TTATGCAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915408.1
CDS	591..5291
Translation	MEAQSRSTTASEKKKVENSIIKGHTRTEVSEKAVASSTTSNEDESPGQTYHRERRNAITMQPHGGQGLGKINEEPSTSSEERASLIKKELHGSITHLPEPSVPYRGTVFAMDPRNGYIDPHYHPPHLFPSFHPPVPIDARHHEGRYHYEPTPIPPLHVPSALASSPTYPDLPFIRISPHRNPASASDSPFSPPHPYISPYMDYIRSLHSSPSLSMISAARGLSPTDVPHAGVSPAEYYHQMALLTGQRSPYADIIPSPATAGAGANALHMEYLHAMESSRFSSPRVPLRPSRKRTLSISPLSDHSFDLQTMIRNSPNSLVTILNNSRSSSSASGSYGHLAASAISPALSFTYQPTPVSLQQMHQQLMSRQHSIGSAFGHSPPLLHPAPTFPSQRTIPGIPSVLNPVQVSIGPSEAAQQNKPTSESAVSSTGDPLHNKRSKVKPEEDLPSPGAVCIQDQPDGMTLVKEEGDKDESKQEPEVVYETNCHWEGCSREFDTQEQLVHHINNDHIHGEKKEFVCRWLDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCSKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDIHPRPPPPREPGSHSQSRSPGQHIQGTHGEHKDLSNTTSKHEECLQVKSVKTEKPMTSQPSPGGKSSCSSEQSPVSNYTNSGIELTLNSGGSIGDFSALDETPIMDSTISTATTGLGLHGRRIMTGTKWMEQIKVEKIKQVNGILPRLNPVPPHRAPNLPPITGNGVQLNSNISLGGSAPVLPNKNELSSMDITVLNMLNRRDSNTSTISSAYMSSRRSSGISPCFSSRRSSEASQFEGRVQNISVADSYDPISTDASRRSSETSQCDGLPSLLSLTPAQQYRLKAKYAAATGGPPPTPLPNMERMSLKTRIALLGDSRDCRLSPLPSVNVPRRCSDGGVGSYGRRHLLSNELQGNGMRRASDPVKMVSDNFSNARVPRFNSLNNITPPDAPPLMERRNLSLQNYTRNVYSPCPPSISENVALEAMIMEAEGNFDDEDLLPDDMVQYLNSRNQRLYDIFETDKLQNNAGERNGNNFEPSHVTSNIINEQFLNSEQTDTIENKNDLPIQWNEVSSGSAEYSPSRLKYGQRLTTQQNRPFGLYNNMMVQQQNVPKNGLPQQRSYQHHTQNNPQVPQQNLNLYNNSSVWSGQSVRGNQCIDIARSSLGTSATGSLCNTTRGQKFTANGLQMNIGQQNYGPSTHYSVQSASRTDPVQNENIMNQEFMHQMTAEHLIYGMHFLGVTRSNQTTSGQNGNTIDATQSFLSTTQNAGEQQPSLAKNIQSFVNSSGNSRQNIHRNNLSYLQEQICDTNQQVCRVNGIKMEMQNHPQQHCANAQNFSGQLYDQTAAYPQQSIKSGSEPGMEANCLLQESNNTNSSQLLSPGANQVTSTVNNIDSSLDEVQIDFDAIIDDGDHASLISGALSPSIIQNLSRNSSRLTTPRTSLTFPAIPVSTSNMAIGDMSSLLTSLAEESKFLAIMQ

Target ORF information:

RefSeq Version	XM_004915351.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis GLI family zinc finger 3 (gli3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915351.4

ORF Insert Sequence:

ATGGAAGCCC AGTCCCGTAG CACTACAGCA AGTGAAAAGA AAAAGGTTGA GAACTCCATA 
ATCAAAGGTC ACACTCGAAC AGAAGTCAGC GAGAAAGCTG TTGCCTCCAG CACAACTTCC 
AATGAGGATG AAAGTCCTGG ACAGACATAT CACAGAGAAA GAAGAAACGC CATCACCATG 
CAGCCTCATG GAGGCCAAGG TCTTGGTAAA ATCAATGAGG AACCCTCTAC GTCAAGTGAG 
GAAAGGGCCT CATTAATAAA AAAAGAACTT CATGGATCTA TAACACATCT GCCTGAACCC 
TCAGTTCCCT ACCGTGGCAC TGTCTTTGCC ATGGACCCTA GGAATGGTTA TATTGATCCA 
CATTACCATC CGCCTCATCT CTTCCCATCT TTTCATCCTC CTGTGCCAAT TGATGCAAGA 
CATCATGAGG GAAGATACCA TTATGAGCCA ACTCCCATTC CGCCACTGCA TGTGCCTTCT 
GCATTAGCAA GCAGCCCGAC ATACCCAGAT CTTCCATTTA TCCGGATATC TCCACACAGA 
AACCCAGCCA GTGCATCTGA TTCCCCATTC AGCCCTCCTC ATCCTTACAT CAGTCCTTAC 
ATGGACTATA TCAGATCTCT CCACAGCAGC CCTTCACTGT CTATGATATC AGCTGCCCGT 
GGACTCAGCC CTACTGATGT CCCACATGCT GGTGTTAGCC CTGCTGAATA TTACCATCAA 
ATGGCACTGC TAACTGGACA GAGAAGCCCA TATGCTGATA TTATTCCATC CCCTGCAACT 
GCTGGTGCTG GAGCTAATGC CCTCCATATG GAATATCTTC ATGCCATGGA GAGCTCACGG 
TTTTCAAGTC CTAGAGTGCC ATTACGACCA AGTCGCAAGA GAACACTGTC CATATCACCT 
CTTTCTGATC ATAGCTTTGA TCTCCAGACA ATGATTCGCA ACTCTCCAAA TTCTCTTGTT 
ACTATACTTA ACAACTCCCG CAGCAGCTCA TCTGCTAGTG GCTCTTATGG GCATCTAGCA 
GCCAGTGCCA TCAGTCCAGC ATTGAGTTTT ACATACCAGC CTACACCAGT GTCTCTTCAG 
CAAATGCATC AACAGCTTAT GAGTCGACAG CACAGCATAG GCTCAGCTTT TGGACACAGC 
CCTCCACTTC TTCACCCAGC ACCAACATTT CCTTCTCAAA GGACCATTCC CGGCATCCCC 
AGTGTTTTGA ATCCAGTGCA GGTCAGCATT GGGCCATCAG AGGCTGCACA GCAAAACAAG 
CCCACCAGTG AGTCAGCAGT CAGCAGCACA GGTGACCCCC TGCACAACAA GCGTTCTAAG 
GTAAAACCTG AAGAAGATCT TCCAAGCCCT GGAGCAGTGT GTATTCAGGA TCAGCCAGAT 
GGAATGACTC TGGTGAAAGA GGAGGGGGAC AAAGATGAAA GTAAGCAAGA GCCTGAAGTG 
GTCTACGAGA CAAACTGCCA TTGGGAAGGC TGCTCGCGAG AGTTTGACAC ACAGGAACAG 
CTAGTGCATC ATATCAACAA TGACCACATA CATGGAGAGA AAAAGGAGTT TGTGTGCCGA 
TGGCTAGATT GTTCAAGAGA ACAAAAGCCG TTTAAGGCTC AGTACATGCT GGTGGTCCAC 
ATGAGAAGAC ATACAGGAGA AAAGCCACAC AAATGCACTT TTGAAGGTTG TTCAAAAGCC 
TACTCCAGAC TAGAAAACTT GAAAACTCAC CTGAGATCTC ACACTGGAGA GAAACCATAT 
GTCTGTGAGC ATGAGGGCTG CAACAAGGCC TTTTCAAATG CATCTGATAG AGCCAAGCAC 
CAAAACCGCA CTCATTCAAA TGAGAAACCT TATGTTTGCA AGATCCCAGG GTGTACAAAA 
CGTTACACTG ACCCTAGCTC ACTTAGAAAG CATGTAAAGA CAGTGCATGG TCCAGAGGCT 
CATGTAACTA AGAAGCAGAG AGGGGACATA CACCCAAGGC CTCCACCACC ACGAGAACCA 
GGAAGCCATT CGCAGTCAAG ATCACCAGGC CAACATATCC AGGGTACACA TGGAGAGCAC 
AAGGACCTCA GTAACACTAC CTCAAAGCAT GAAGAATGCC TGCAAGTAAA ATCAGTCAAG 
ACTGAAAAGC CAATGACATC TCAGCCAAGC CCTGGTGGTA AGTCTTCATG CAGCAGCGAG 
CAGTCCCCCG TCAGCAACTA TACCAACAGT GGGATCGAGC TTACTCTAAA CAGTGGAGGC 
AGCATTGGAG ACTTCAGTGC CCTAGATGAA ACCCCAATCA TGGACTCTAC CATTTCCACC 
GCAACCACAG GACTTGGTTT ACACGGAAGG AGGATTATGA CAGGAACCAA ATGGATGGAG 
CAAATTAAAG TTGAAAAAAT TAAACAAGTT AATGGTATTC TTCCAAGACT GAACCCAGTT 
CCACCTCATA GAGCACCAAA TTTACCACCA ATCACTGGAA ATGGTGTGCA GCTGAACAGC 
AACATCAGTT TGGGGGGATC AGCACCTGTT CTTCCAAACA AAAATGAGCT TTCTAGCATG 
GACATTACAG TCTTGAATAT GCTTAACAGA AGAGATAGCA ATACTAGCAC AATAAGTTCA 
GCATATATGA GCAGCCGCAG GTCCTCTGGA ATTTCACCTT GTTTTTCCAG TCGAAGATCC 
AGTGAGGCAT CTCAGTTTGA GGGACGGGTT CAGAATATAA GTGTTGCAGA CTCCTATGAT 
CCAATTTCTA CTGATGCCTC AAGACGATCA AGTGAAACAA GCCAGTGTGA TGGGCTGCCA 
AGCCTCCTAA GCCTTACTCC AGCACAGCAA TACAGGCTGA AAGCTAAATA TGCTGCTGCT 
ACAGGTGGGC CCCCACCAAC ACCACTGCCG AACATGGAAA GAATGAGTCT AAAAACCAGA 
ATAGCTCTCC TGGGTGACTC AAGAGATTGT AGATTGTCAC CTCTTCCTTC AGTCAATGTC 
CCACGAAGAT GTAGTGATGG TGGAGTAGGC TCTTATGGAA GAAGGCATTT GTTGTCAAAT 
GAACTTCAAG GTAATGGAAT GCGTAGAGCA AGTGATCCTG TAAAAATGGT CTCTGATAAC 
TTTTCTAATG CTAGAGTTCC AAGGTTCAAC AGTCTTAACA ACATCACCCC TCCTGATGCC 
CCGCCACTTA TGGAAAGGCG CAATTTAAGT CTTCAGAACT ATACCCGTAA TGTGTATTCA 
CCATGCCCTC CTAGTATAAG TGAAAATGTT GCCCTGGAAG CAATGATAAT GGAAGCAGAA 
GGCAATTTTG ATGATGAGGA TTTACTGCCA GATGACATGG TTCAGTATCT TAACTCTCGG 
AACCAAAGAC TTTATGACAT TTTTGAAACA GATAAATTAC AGAATAATGC TGGAGAAAGA 
AATGGAAACA ACTTTGAACC ATCTCATGTA ACCAGTAATA TAATAAATGA ACAGTTCCTC 
AATTCCGAAC AAACAGATAC CATAGAAAAT AAAAATGACC TCCCAATTCA GTGGAATGAA 
GTAAGCTCTG GCAGTGCAGA ATATTCTCCA TCGAGATTAA AGTATGGGCA GCGTTTAACC 
ACACAGCAGA ATCGACCTTT TGGATTGTAT AATAACATGA TGGTCCAGCA GCAAAATGTA 
CCAAAGAACG GGTTGCCCCA GCAAAGGAGC TATCAACATC ATACACAAAA CAACCCACAG 
GTGCCCCAGC AAAATCTGAA CCTTTACAAT AACAGCAGTG TGTGGAGTGG TCAGTCAGTC 
AGGGGCAATC AGTGCATTGA CATAGCAAGA TCATCACTGG GAACGTCTGC AACAGGAAGT 
CTTTGCAATA CAACTCGAGG ACAAAAATTT ACAGCCAATG GTTTGCAAAT GAATATTGGT 
CAACAAAACT ATGGACCATC CACACATTAT AGTGTGCAAT CAGCTAGCAG AACTGACCCA 
GTCCAGAATG AAAACATCAT GAATCAGGAA TTTATGCACC AGATGACAGC CGAACACCTA 
ATTTATGGTA TGCATTTCCT GGGGGTAACT CGATCTAATC AGACAACATC AGGGCAAAAT 
GGAAACACAA TTGATGCAAC ACAAAGTTTC CTGTCAACTA CTCAAAATGC TGGAGAGCAG 
CAGCCATCCC TGGCAAAGAA TATCCAGTCT TTTGTAAACT CTAGTGGAAA TAGCAGGCAG 
AATATACATA GAAACAACCT GTCATATCTG CAAGAGCAAA TATGTGACAC CAACCAACAA 
GTTTGCAGAG TGAATGGTAT TAAAATGGAA ATGCAGAATC ACCCACAACA GCACTGTGCG 
AATGCACAAA ACTTCTCCGG TCAGTTGTAT GACCAAACAG CAGCATATCC TCAGCAAAGT 
ATCAAGTCAG GTTCTGAGCC TGGTATGGAA GCAAACTGTC TTTTACAAGA AAGTAATAAT 
ACAAACTCAT CCCAGCTTCT TTCGCCTGGG GCAAACCAAG TGACTAGCAC AGTCAATAAC 
ATTGACAGCA GTTTGGATGA GGTGCAAATC GATTTTGATG CTATAATTGA TGATGGGGAT 
CATGCCAGCC TCATTTCTGG AGCTCTAAGT CCAAGTATCA TCCAGAATCT CTCCCGTAAT 
TCTTCACGCC TTACCACTCC TCGAACATCT CTGACATTCC CAGCCATACC TGTTAGTACC 
AGTAACATGG CTATTGGGGA CATGAGCTCT TTGCTGACCT CACTTGCAGA AGAAAGCAAG 
TTTCTTGCTA TTATGCAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gli3 ( XM_012965492.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012820946.1 Xenopus tropicalis GLI family zinc finger 3 (gli3), transcript variant X3, mRNA.

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CloneID	OXa30279
Clone ID Related Accession (Same CDS sequence)	XM_012965492.2 , XM_012965492.3
Accession Version	XM_012965492.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transcriptional activator GLI3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965492.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCTC ATGGAGGCCA AGGTCTTGGT AAAATCAATG AGGAACCCTC TACGTCAAGT 
GAGGAAAGGG CCTCATTAAT AAAAAAAGAA CTTCATGGAT CTATAACACA TCTGCCTGAA 
CCCTCAGTTC CCTACCGTGG CACTGTCTTT GCCATGGACC CTAGGAATGG TTATATTGAT 
CCACATTACC ATCCGCCTCA TCTCTTCCCA TCTTTTCATC CTCCTGTGCC AATTGATGCA 
AGACATCATG AGGGAAGATA CCATTATGAG CCAACTCCCA TTCCGCCACT GCATGTGCCT 
TCTGCATTAG CAAGCAGCCC GACATACCCA GATCTTCCAT TTATCCGGAT ATCTCCACAC 
AGAAACCCAG CCAGTGCATC TGATTCCCCA TTCAGCCCTC CTCATCCTTA CATCAGTCCT 
TACATGGACT ATATCAGATC TCTCCACAGC AGCCCTTCAC TGTCTATGAT ATCAGCTGCC 
CGTGGACTCA GCCCTACTGA TGTCCCACAT GCTGGTGTTA GCCCTGCTGA ATATTACCAT 
CAAATGGCAC TGCTAACTGG ACAGAGAAGC CCATATGCTG ATATTATTCC ATCCCCTGCA 
ACTGCTGGTG CTGGAGCTAA TGCCCTCCAT ATGGAATATC TTCATGCCAT GGAGAGCTCA 
CGGTTTTCAA GTCCTAGAGT GCCATTACGA CCAAGTCGCA AGAGAACACT GTCCATATCA 
CCTCTTTCTG ATCATAGCTT TGATCTCCAG ACAATGATTC GCAACTCTCC AAATTCTCTT 
GTTACTATAC TTAACAACTC CCGCAGCAGC TCATCTGCTA GTGGCTCTTA TGGGCATCTA 
GCAGCCAGTG CCATCAGTCC AGCATTGAGT TTTACATACC AGCCTACACC AGTGTCTCTT 
CAGCAAATGC ATCAACAGCT TATGAGTCGA CAGCACAGCA TAGGCTCAGC TTTTGGACAC 
AGCCCTCCAC TTCTTCACCC AGCACCAACA TTTCCTTCTC AAAGGACCAT TCCCGGCATC 
CCCAGTGTTT TGAATCCAGT GCAGGTCAGC ATTGGGCCAT CAGAGGCTGC ACAGCAAAAC 
AAGCCCACCA GTGAGTCAGC AGTCAGCAGC ACAGGTGACC CCCTGCACAA CAAGCGTTCT 
AAGGTAAAAC CTGAAGAAGA TCTTCCAAGC CCTGGAGCAG TGTGTATTCA GGATCAGCCA 
GATGGAATGA CTCTGGTGAA AGAGGAGGGG GACAAAGATG AAAGTAAGCA AGAGCCTGAA 
GTGGTCTACG AGACAAACTG CCATTGGGAA GGCTGCTCGC GAGAGTTTGA CACACAGGAA 
CAGCTAGTGC ATCATATCAA CAATGACCAC ATACATGGAG AGAAAAAGGA GTTTGTGTGC 
CGATGGCTAG ATTGTTCAAG AGAACAAAAG CCGTTTAAGG CTCAGTACAT GCTGGTGGTC 
CACATGAGAA GACATACAGG AGAAAAGCCA CACAAATGCA CTTTTGAAGG TTGTTCAAAA 
GCCTACTCCA GACTAGAAAA CTTGAAAACT CACCTGAGAT CTCACACTGG AGAGAAACCA 
TATGTCTGTG AGCATGAGGG CTGCAACAAG GCCTTTTCAA ATGCATCTGA TAGAGCCAAG 
CACCAAAACC GCACTCATTC AAATGAGAAA CCTTATGTTT GCAAGATCCC AGGGTGTACA 
AAACGTTACA CTGACCCTAG CTCACTTAGA AAGCATGTAA AGACAGTGCA TGGTCCAGAG 
GCTCATGTAA CTAAGAAGCA GAGAGGGGAC ATACACCCAA GGCCTCCACC ACCACGAGAA 
CCAGGAAGCC ATTCGCAGTC AAGATCACCA GGCCAACATA TCCAGGGTAC ACATGGAGAG 
CACAAGGACC TCAGTAACAC TACCTCAAAG CATGAAGAAT GCCTGCAAGT AAAATCAGTC 
AAGACTGAAA AGCCAATGAC ATCTCAGCCA AGCCCTGGTG GTAAGTCTTC ATGCAGCAGC 
GAGCAGTCCC CCGTCAGCAA CTATACCAAC AGTGGGATCG AGCTTACTCT AAACAGTGGA 
GGCAGCATTG GAGACTTCAG TGCCCTAGAT GAAACCCCAA TCATGGACTC TACCATTTCC 
ACCGCAACCA CAGGACTTGG TTTACACGGA AGGAGGATTA TGACAGGAAC CAAATGGATG 
GAGCAAATTA AAGTTGAAAA AATTAAACAA GTTAATGGTA TTCTTCCAAG ACTGAACCCA 
GTTCCACCTC ATAGAGCACC AAATTTACCA CCAATCACTG GAAATGGTGT GCAGCTGAAC 
AGCAACATCA GTTTGGGGGG ATCAGCACCT GTTCTTCCAA ACAAAAATGA GCTTTCTAGC 
ATGGACATTA CAGTCTTGAA TATGCTTAAC AGAAGAGATA GCAATACTAG CACAATAAGT 
TCAGCATATA TGAGCAGCCG CAGGTCCTCT GGAATTTCAC CTTGTTTTTC CAGTCGAAGA 
TCCAGTGAGG CATCTCAGTT TGAGGGACGG GTTCAGAATA TAAGTGTTGC AGACTCCTAT 
GATCCAATTT CTACTGATGC CTCAAGACGA TCAAGTGAAA CAAGCCAGTG TGATGGGCTG 
CCAAGCCTCC TAAGCCTTAC TCCAGCACAG CAATACAGGC TGAAAGCTAA ATATGCTGCT 
GCTACAGGTG GGCCCCCACC AACACCACTG CCGAACATGG AAAGAATGAG TCTAAAAACC 
AGAATAGCTC TCCTGGGTGA CTCAAGAGAT TGTAGATTGT CACCTCTTCC TTCAGTCAAT 
GTCCCACGAA GATGTAGTGA TGGTGGAGTA GGCTCTTATG GAAGAAGGCA TTTGTTGTCA 
AATGAACTTC AAGGTAATGG AATGCGTAGA GCAAGTGATC CTGTAAAAAT GGTCTCTGAT 
AACTTTTCTA ATGCTAGAGT TCCAAGGTTC AACAGTCTTA ACAACATCAC CCCTCCTGAT 
GCCCCGCCAC TTATGGAAAG GCGCAATTTA AGTCTTCAGA ACTATACCCG TAATGTGTAT 
TCACCATGCC CTCCTAGTAT AAGTGAAAAT GTTGCCCTGG AAGCAATGAT AATGGAAGCA 
GAAGGCAATT TTGATGATGA GGATTTACTG CCAGATGACA TGGTTCAGTA TCTTAACTCT 
CGGAACCAAA GACTTTATGA CATTTTTGAA ACAGATAAAT TACAGAATAA TGCTGGAGAA 
AGAAATGGAA ACAACTTTGA ACCATCTCAT GTAACCAGTA ATATAATAAA TGAACAGTTC 
CTCAATTCCG AACAAACAGA TACCATAGAA AATAAAAATG ACCTCCCAAT TCAGTGGAAT 
GAAGTAAGCT CTGGCAGTGC AGAATATTCT CCATCGAGAT TAAAGTATGG GCAGCGTTTA 
ACCACACAGC AGAATCGACC TTTTGGATTG TATAATAACA TGATGGTCCA GCAGCAAAAT 
GTACCAAAGA ACGGGTTGCC CCAGCAAAGG AGCTATCAAC ATCATACACA AAACAACCCA 
CAGGTGCCCC AGCAAAATCT GAACCTTTAC AATAACAGCA GTGTGTGGAG TGGTCAGTCA 
GTCAGGGGCA ATCAGTGCAT TGACATAGCA AGATCATCAC TGGGAACGTC TGCAACAGGA 
AGTCTTTGCA ATACAACTCG AGGACAAAAA TTTACAGCCA ATGGTTTGCA AATGAATATT 
GGTCAACAAA ACTATGGACC ATCCACACAT TATAGTGTGC AATCAGCTAG CAGAACTGAC 
CCAGTCCAGA ATGAAAACAT CATGAATCAG GAATTTATGC ACCAGATGAC AGCCGAACAC 
CTAATTTATG GTATGCATTT CCTGGGGGTA ACTCGATCTA ATCAGACAAC ATCAGGGCAA 
AATGGAAACA CAATTGATGC AACACAAAGT TTCCTGTCAA CTACTCAAAA TGCTGGAGAG 
CAGCAGCCAT CCCTGGCAAA GAATATCCAG TCTTTTGTAA ACTCTAGTGG AAATAGCAGG 
CAGAATATAC ATAGAAACAA CCTGTCATAT CTGCAAGAGC AAATATGTGA CACCAACCAA 
CAAGTTTGCA GAGTGAATGG TATTAAAATG GAAATGCAGA ATCACCCACA ACAGCACTGT 
GCGAATGCAC AAAACTTCTC CGGTCAGTTG TATGACCAAA CAGCAGCATA TCCTCAGCAA 
AGTATCAAGT CAGGTTCTGA GCCTGGTATG GAAGCAAACT GTCTTTTACA AGAAAGTAAT 
AATACAAACT CATCCCAGCT TCTTTCGCCT GGGGCAAACC AAGTGACTAG CACAGTCAAT 
AACATTGACA GCAGTTTGGA TGAGGTGCAA ATCGATTTTG ATGCTATAAT TGATGATGGG 
GATCATGCCA GCCTCATTTC TGGAGCTCTA AGTCCAAGTA TCATCCAGAA TCTCTCCCGT 
AATTCTTCAC GCCTTACCAC TCCTCGAACA TCTCTGACAT TCCCAGCCAT ACCTGTTAGT 
ACCAGTAACA TGGCTATTGG GGACATGAGC TCTTTGCTGA CCTCACTTGC AGAAGAAAGC 
AAGTTTCTTG CTATTATGCA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012820946.1
CDS	167..4690
Translation	MQPHGGQGLGKINEEPSTSSEERASLIKKELHGSITHLPEPSVPYRGTVFAMDPRNGYIDPHYHPPHLFPSFHPPVPIDARHHEGRYHYEPTPIPPLHVPSALASSPTYPDLPFIRISPHRNPASASDSPFSPPHPYISPYMDYIRSLHSSPSLSMISAARGLSPTDVPHAGVSPAEYYHQMALLTGQRSPYADIIPSPATAGAGANALHMEYLHAMESSRFSSPRVPLRPSRKRTLSISPLSDHSFDLQTMIRNSPNSLVTILNNSRSSSSASGSYGHLAASAISPALSFTYQPTPVSLQQMHQQLMSRQHSIGSAFGHSPPLLHPAPTFPSQRTIPGIPSVLNPVQVSIGPSEAAQQNKPTSESAVSSTGDPLHNKRSKVKPEEDLPSPGAVCIQDQPDGMTLVKEEGDKDESKQEPEVVYETNCHWEGCSREFDTQEQLVHHINNDHIHGEKKEFVCRWLDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCSKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDIHPRPPPPREPGSHSQSRSPGQHIQGTHGEHKDLSNTTSKHEECLQVKSVKTEKPMTSQPSPGGKSSCSSEQSPVSNYTNSGIELTLNSGGSIGDFSALDETPIMDSTISTATTGLGLHGRRIMTGTKWMEQIKVEKIKQVNGILPRLNPVPPHRAPNLPPITGNGVQLNSNISLGGSAPVLPNKNELSSMDITVLNMLNRRDSNTSTISSAYMSSRRSSGISPCFSSRRSSEASQFEGRVQNISVADSYDPISTDASRRSSETSQCDGLPSLLSLTPAQQYRLKAKYAAATGGPPPTPLPNMERMSLKTRIALLGDSRDCRLSPLPSVNVPRRCSDGGVGSYGRRHLLSNELQGNGMRRASDPVKMVSDNFSNARVPRFNSLNNITPPDAPPLMERRNLSLQNYTRNVYSPCPPSISENVALEAMIMEAEGNFDDEDLLPDDMVQYLNSRNQRLYDIFETDKLQNNAGERNGNNFEPSHVTSNIINEQFLNSEQTDTIENKNDLPIQWNEVSSGSAEYSPSRLKYGQRLTTQQNRPFGLYNNMMVQQQNVPKNGLPQQRSYQHHTQNNPQVPQQNLNLYNNSSVWSGQSVRGNQCIDIARSSLGTSATGSLCNTTRGQKFTANGLQMNIGQQNYGPSTHYSVQSASRTDPVQNENIMNQEFMHQMTAEHLIYGMHFLGVTRSNQTTSGQNGNTIDATQSFLSTTQNAGEQQPSLAKNIQSFVNSSGNSRQNIHRNNLSYLQEQICDTNQQVCRVNGIKMEMQNHPQQHCANAQNFSGQLYDQTAAYPQQSIKSGSEPGMEANCLLQESNNTNSSQLLSPGANQVTSTVNNIDSSLDEVQIDFDAIIDDGDHASLISGALSPSIIQNLSRNSSRLTTPRTSLTFPAIPVSTSNMAIGDMSSLLTSLAEESKFLAIMQ

Target ORF information:

RefSeq Version	XM_012965492.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis GLI family zinc finger 3 (gli3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965492.3

ORF Insert Sequence:

ATGCAGCCTC ATGGAGGCCA AGGTCTTGGT AAAATCAATG AGGAACCCTC TACGTCAAGT 
GAGGAAAGGG CCTCATTAAT AAAAAAAGAA CTTCATGGAT CTATAACACA TCTGCCTGAA 
CCCTCAGTTC CCTACCGTGG CACTGTCTTT GCCATGGACC CTAGGAATGG TTATATTGAT 
CCACATTACC ATCCGCCTCA TCTCTTCCCA TCTTTTCATC CTCCTGTGCC AATTGATGCA 
AGACATCATG AGGGAAGATA CCATTATGAG CCAACTCCCA TTCCGCCACT GCATGTGCCT 
TCTGCATTAG CAAGCAGCCC GACATACCCA GATCTTCCAT TTATCCGGAT ATCTCCACAC 
AGAAACCCAG CCAGTGCATC TGATTCCCCA TTCAGCCCTC CTCATCCTTA CATCAGTCCT 
TACATGGACT ATATCAGATC TCTCCACAGC AGCCCTTCAC TGTCTATGAT ATCAGCTGCC 
CGTGGACTCA GCCCTACTGA TGTCCCACAT GCTGGTGTTA GCCCTGCTGA ATATTACCAT 
CAAATGGCAC TGCTAACTGG ACAGAGAAGC CCATATGCTG ATATTATTCC ATCCCCTGCA 
ACTGCTGGTG CTGGAGCTAA TGCCCTCCAT ATGGAATATC TTCATGCCAT GGAGAGCTCA 
CGGTTTTCAA GTCCTAGAGT GCCATTACGA CCAAGTCGCA AGAGAACACT GTCCATATCA 
CCTCTTTCTG ATCATAGCTT TGATCTCCAG ACAATGATTC GCAACTCTCC AAATTCTCTT 
GTTACTATAC TTAACAACTC CCGCAGCAGC TCATCTGCTA GTGGCTCTTA TGGGCATCTA 
GCAGCCAGTG CCATCAGTCC AGCATTGAGT TTTACATACC AGCCTACACC AGTGTCTCTT 
CAGCAAATGC ATCAACAGCT TATGAGTCGA CAGCACAGCA TAGGCTCAGC TTTTGGACAC 
AGCCCTCCAC TTCTTCACCC AGCACCAACA TTTCCTTCTC AAAGGACCAT TCCCGGCATC 
CCCAGTGTTT TGAATCCAGT GCAGGTCAGC ATTGGGCCAT CAGAGGCTGC ACAGCAAAAC 
AAGCCCACCA GTGAGTCAGC AGTCAGCAGC ACAGGTGACC CCCTGCACAA CAAGCGTTCT 
AAGGTAAAAC CTGAAGAAGA TCTTCCAAGC CCTGGAGCAG TGTGTATTCA GGATCAGCCA 
GATGGAATGA CTCTGGTGAA AGAGGAGGGG GACAAAGATG AAAGTAAGCA AGAGCCTGAA 
GTGGTCTACG AGACAAACTG CCATTGGGAA GGCTGCTCGC GAGAGTTTGA CACACAGGAA 
CAGCTAGTGC ATCATATCAA CAATGACCAC ATACATGGAG AGAAAAAGGA GTTTGTGTGC 
CGATGGCTAG ATTGTTCAAG AGAACAAAAG CCGTTTAAGG CTCAGTACAT GCTGGTGGTC 
CACATGAGAA GACATACAGG AGAAAAGCCA CACAAATGCA CTTTTGAAGG TTGTTCAAAA 
GCCTACTCCA GACTAGAAAA CTTGAAAACT CACCTGAGAT CTCACACTGG AGAGAAACCA 
TATGTCTGTG AGCATGAGGG CTGCAACAAG GCCTTTTCAA ATGCATCTGA TAGAGCCAAG 
CACCAAAACC GCACTCATTC AAATGAGAAA CCTTATGTTT GCAAGATCCC AGGGTGTACA 
AAACGTTACA CTGACCCTAG CTCACTTAGA AAGCATGTAA AGACAGTGCA TGGTCCAGAG 
GCTCATGTAA CTAAGAAGCA GAGAGGGGAC ATACACCCAA GGCCTCCACC ACCACGAGAA 
CCAGGAAGCC ATTCGCAGTC AAGATCACCA GGCCAACATA TCCAGGGTAC ACATGGAGAG 
CACAAGGACC TCAGTAACAC TACCTCAAAG CATGAAGAAT GCCTGCAAGT AAAATCAGTC 
AAGACTGAAA AGCCAATGAC ATCTCAGCCA AGCCCTGGTG GTAAGTCTTC ATGCAGCAGC 
GAGCAGTCCC CCGTCAGCAA CTATACCAAC AGTGGGATCG AGCTTACTCT AAACAGTGGA 
GGCAGCATTG GAGACTTCAG TGCCCTAGAT GAAACCCCAA TCATGGACTC TACCATTTCC 
ACCGCAACCA CAGGACTTGG TTTACACGGA AGGAGGATTA TGACAGGAAC CAAATGGATG 
GAGCAAATTA AAGTTGAAAA AATTAAACAA GTTAATGGTA TTCTTCCAAG ACTGAACCCA 
GTTCCACCTC ATAGAGCACC AAATTTACCA CCAATCACTG GAAATGGTGT GCAGCTGAAC 
AGCAACATCA GTTTGGGGGG ATCAGCACCT GTTCTTCCAA ACAAAAATGA GCTTTCTAGC 
ATGGACATTA CAGTCTTGAA TATGCTTAAC AGAAGAGATA GCAATACTAG CACAATAAGT 
TCAGCATATA TGAGCAGCCG CAGGTCCTCT GGAATTTCAC CTTGTTTTTC CAGTCGAAGA 
TCCAGTGAGG CATCTCAGTT TGAGGGACGG GTTCAGAATA TAAGTGTTGC AGACTCCTAT 
GATCCAATTT CTACTGATGC CTCAAGACGA TCAAGTGAAA CAAGCCAGTG TGATGGGCTG 
CCAAGCCTCC TAAGCCTTAC TCCAGCACAG CAATACAGGC TGAAAGCTAA ATATGCTGCT 
GCTACAGGTG GGCCCCCACC AACACCACTG CCGAACATGG AAAGAATGAG TCTAAAAACC 
AGAATAGCTC TCCTGGGTGA CTCAAGAGAT TGTAGATTGT CACCTCTTCC TTCAGTCAAT 
GTCCCACGAA GATGTAGTGA TGGTGGAGTA GGCTCTTATG GAAGAAGGCA TTTGTTGTCA 
AATGAACTTC AAGGTAATGG AATGCGTAGA GCAAGTGATC CTGTAAAAAT GGTCTCTGAT 
AACTTTTCTA ATGCTAGAGT TCCAAGGTTC AACAGTCTTA ACAACATCAC CCCTCCTGAT 
GCCCCGCCAC TTATGGAAAG GCGCAATTTA AGTCTTCAGA ACTATACCCG TAATGTGTAT 
TCACCATGCC CTCCTAGTAT AAGTGAAAAT GTTGCCCTGG AAGCAATGAT AATGGAAGCA 
GAAGGCAATT TTGATGATGA GGATTTACTG CCAGATGACA TGGTTCAGTA TCTTAACTCT 
CGGAACCAAA GACTTTATGA CATTTTTGAA ACAGATAAAT TACAGAATAA TGCTGGAGAA 
AGAAATGGAA ACAACTTTGA ACCATCTCAT GTAACCAGTA ATATAATAAA TGAACAGTTC 
CTCAATTCCG AACAAACAGA TACCATAGAA AATAAAAATG ACCTCCCAAT TCAGTGGAAT 
GAAGTAAGCT CTGGCAGTGC AGAATATTCT CCATCGAGAT TAAAGTATGG GCAGCGTTTA 
ACCACACAGC AGAATCGACC TTTTGGATTG TATAATAACA TGATGGTCCA GCAGCAAAAT 
GTACCAAAGA ACGGGTTGCC CCAGCAAAGG AGCTATCAAC ATCATACACA AAACAACCCA 
CAGGTGCCCC AGCAAAATCT GAACCTTTAC AATAACAGCA GTGTGTGGAG TGGTCAGTCA 
GTCAGGGGCA ATCAGTGCAT TGACATAGCA AGATCATCAC TGGGAACGTC TGCAACAGGA 
AGTCTTTGCA ATACAACTCG AGGACAAAAA TTTACAGCCA ATGGTTTGCA AATGAATATT 
GGTCAACAAA ACTATGGACC ATCCACACAT TATAGTGTGC AATCAGCTAG CAGAACTGAC 
CCAGTCCAGA ATGAAAACAT CATGAATCAG GAATTTATGC ACCAGATGAC AGCCGAACAC 
CTAATTTATG GTATGCATTT CCTGGGGGTA ACTCGATCTA ATCAGACAAC ATCAGGGCAA 
AATGGAAACA CAATTGATGC AACACAAAGT TTCCTGTCAA CTACTCAAAA TGCTGGAGAG 
CAGCAGCCAT CCCTGGCAAA GAATATCCAG TCTTTTGTAA ACTCTAGTGG AAATAGCAGG 
CAGAATATAC ATAGAAACAA CCTGTCATAT CTGCAAGAGC AAATATGTGA CACCAACCAA 
CAAGTTTGCA GAGTGAATGG TATTAAAATG GAAATGCAGA ATCACCCACA ACAGCACTGT 
GCGAATGCAC AAAACTTCTC CGGTCAGTTG TATGACCAAA CAGCAGCATA TCCTCAGCAA 
AGTATCAAGT CAGGTTCTGA GCCTGGTATG GAAGCAAACT GTCTTTTACA AGAAAGTAAT 
AATACAAACT CATCCCAGCT TCTTTCGCCT GGGGCAAACC AAGTGACTAG CACAGTCAAT 
AACATTGACA GCAGTTTGGA TGAGGTGCAA ATCGATTTTG ATGCTATAAT TGATGATGGG 
GATCATGCCA GCCTCATTTC TGGAGCTCTA AGTCCAAGTA TCATCCAGAA TCTCTCCCGT 
AATTCTTCAC GCCTTACCAC TCCTCGAACA TCTCTGACAT TCCCAGCCAT ACCTGTTAGT 
ACCAGTAACA TGGCTATTGG GGACATGAGC TCTTTGCTGA CCTCACTTGC AGAAGAAAGC 
AAGTTTCTTG CTATTATGCA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gli3 ( XM_004915350.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915407.1 Xenopus tropicalis GLI family zinc finger 3 (gli3), transcript variant X2, mRNA.

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CloneID	OXa11836
Clone ID Related Accession (Same CDS sequence)	XM_004915350.3 , XM_004915350.4 , XM_004915351.4 , XM_004915351.3
Accession Version	XM_004915350.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transcriptional activator GLI3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004915350.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCCC AGTCCCGTAG CACTACAGCA AGTGAAAAGA AAAAGGTTGA GAACTCCATA 
ATCAAAGGTC ACACTCGAAC AGAAGTCAGC GAGAAAGCTG TTGCCTCCAG CACAACTTCC 
AATGAGGATG AAAGTCCTGG ACAGACATAT CACAGAGAAA GAAGAAACGC CATCACCATG 
CAGCCTCATG GAGGCCAAGG TCTTGGTAAA ATCAATGAGG AACCCTCTAC GTCAAGTGAG 
GAAAGGGCCT CATTAATAAA AAAAGAACTT CATGGATCTA TAACACATCT GCCTGAACCC 
TCAGTTCCCT ACCGTGGCAC TGTCTTTGCC ATGGACCCTA GGAATGGTTA TATTGATCCA 
CATTACCATC CGCCTCATCT CTTCCCATCT TTTCATCCTC CTGTGCCAAT TGATGCAAGA 
CATCATGAGG GAAGATACCA TTATGAGCCA ACTCCCATTC CGCCACTGCA TGTGCCTTCT 
GCATTAGCAA GCAGCCCGAC ATACCCAGAT CTTCCATTTA TCCGGATATC TCCACACAGA 
AACCCAGCCA GTGCATCTGA TTCCCCATTC AGCCCTCCTC ATCCTTACAT CAGTCCTTAC 
ATGGACTATA TCAGATCTCT CCACAGCAGC CCTTCACTGT CTATGATATC AGCTGCCCGT 
GGACTCAGCC CTACTGATGT CCCACATGCT GGTGTTAGCC CTGCTGAATA TTACCATCAA 
ATGGCACTGC TAACTGGACA GAGAAGCCCA TATGCTGATA TTATTCCATC CCCTGCAACT 
GCTGGTGCTG GAGCTAATGC CCTCCATATG GAATATCTTC ATGCCATGGA GAGCTCACGG 
TTTTCAAGTC CTAGAGTGCC ATTACGACCA AGTCGCAAGA GAACACTGTC CATATCACCT 
CTTTCTGATC ATAGCTTTGA TCTCCAGACA ATGATTCGCA ACTCTCCAAA TTCTCTTGTT 
ACTATACTTA ACAACTCCCG CAGCAGCTCA TCTGCTAGTG GCTCTTATGG GCATCTAGCA 
GCCAGTGCCA TCAGTCCAGC ATTGAGTTTT ACATACCAGC CTACACCAGT GTCTCTTCAG 
CAAATGCATC AACAGCTTAT GAGTCGACAG CACAGCATAG GCTCAGCTTT TGGACACAGC 
CCTCCACTTC TTCACCCAGC ACCAACATTT CCTTCTCAAA GGACCATTCC CGGCATCCCC 
AGTGTTTTGA ATCCAGTGCA GGTCAGCATT GGGCCATCAG AGGCTGCACA GCAAAACAAG 
CCCACCAGTG AGTCAGCAGT CAGCAGCACA GGTGACCCCC TGCACAACAA GCGTTCTAAG 
GTAAAACCTG AAGAAGATCT TCCAAGCCCT GGAGCAGTGT GTATTCAGGA TCAGCCAGAT 
GGAATGACTC TGGTGAAAGA GGAGGGGGAC AAAGATGAAA GTAAGCAAGA GCCTGAAGTG 
GTCTACGAGA CAAACTGCCA TTGGGAAGGC TGCTCGCGAG AGTTTGACAC ACAGGAACAG 
CTAGTGCATC ATATCAACAA TGACCACATA CATGGAGAGA AAAAGGAGTT TGTGTGCCGA 
TGGCTAGATT GTTCAAGAGA ACAAAAGCCG TTTAAGGCTC AGTACATGCT GGTGGTCCAC 
ATGAGAAGAC ATACAGGAGA AAAGCCACAC AAATGCACTT TTGAAGGTTG TTCAAAAGCC 
TACTCCAGAC TAGAAAACTT GAAAACTCAC CTGAGATCTC ACACTGGAGA GAAACCATAT 
GTCTGTGAGC ATGAGGGCTG CAACAAGGCC TTTTCAAATG CATCTGATAG AGCCAAGCAC 
CAAAACCGCA CTCATTCAAA TGAGAAACCT TATGTTTGCA AGATCCCAGG GTGTACAAAA 
CGTTACACTG ACCCTAGCTC ACTTAGAAAG CATGTAAAGA CAGTGCATGG TCCAGAGGCT 
CATGTAACTA AGAAGCAGAG AGGGGACATA CACCCAAGGC CTCCACCACC ACGAGAACCA 
GGAAGCCATT CGCAGTCAAG ATCACCAGGC CAACATATCC AGGGTACACA TGGAGAGCAC 
AAGGACCTCA GTAACACTAC CTCAAAGCAT GAAGAATGCC TGCAAGTAAA ATCAGTCAAG 
ACTGAAAAGC CAATGACATC TCAGCCAAGC CCTGGTGGTA AGTCTTCATG CAGCAGCGAG 
CAGTCCCCCG TCAGCAACTA TACCAACAGT GGGATCGAGC TTACTCTAAA CAGTGGAGGC 
AGCATTGGAG ACTTCAGTGC CCTAGATGAA ACCCCAATCA TGGACTCTAC CATTTCCACC 
GCAACCACAG GACTTGGTTT ACACGGAAGG AGGATTATGA CAGGAACCAA ATGGATGGAG 
CAAATTAAAG TTGAAAAAAT TAAACAAGTT AATGGTATTC TTCCAAGACT GAACCCAGTT 
CCACCTCATA GAGCACCAAA TTTACCACCA ATCACTGGAA ATGGTGTGCA GCTGAACAGC 
AACATCAGTT TGGGGGGATC AGCACCTGTT CTTCCAAACA AAAATGAGCT TTCTAGCATG 
GACATTACAG TCTTGAATAT GCTTAACAGA AGAGATAGCA ATACTAGCAC AATAAGTTCA 
GCATATATGA GCAGCCGCAG GTCCTCTGGA ATTTCACCTT GTTTTTCCAG TCGAAGATCC 
AGTGAGGCAT CTCAGTTTGA GGGACGGGTT CAGAATATAA GTGTTGCAGA CTCCTATGAT 
CCAATTTCTA CTGATGCCTC AAGACGATCA AGTGAAACAA GCCAGTGTGA TGGGCTGCCA 
AGCCTCCTAA GCCTTACTCC AGCACAGCAA TACAGGCTGA AAGCTAAATA TGCTGCTGCT 
ACAGGTGGGC CCCCACCAAC ACCACTGCCG AACATGGAAA GAATGAGTCT AAAAACCAGA 
ATAGCTCTCC TGGGTGACTC AAGAGATTGT AGATTGTCAC CTCTTCCTTC AGTCAATGTC 
CCACGAAGAT GTAGTGATGG TGGAGTAGGC TCTTATGGAA GAAGGCATTT GTTGTCAAAT 
GAACTTCAAG GTAATGGAAT GCGTAGAGCA AGTGATCCTG TAAAAATGGT CTCTGATAAC 
TTTTCTAATG CTAGAGTTCC AAGGTTCAAC AGTCTTAACA ACATCACCCC TCCTGATGCC 
CCGCCACTTA TGGAAAGGCG CAATTTAAGT CTTCAGAACT ATACCCGTAA TGTGTATTCA 
CCATGCCCTC CTAGTATAAG TGAAAATGTT GCCCTGGAAG CAATGATAAT GGAAGCAGAA 
GGCAATTTTG ATGATGAGGA TTTACTGCCA GATGACATGG TTCAGTATCT TAACTCTCGG 
AACCAAAGAC TTTATGACAT TTTTGAAACA GATAAATTAC AGAATAATGC TGGAGAAAGA 
AATGGAAACA ACTTTGAACC ATCTCATGTA ACCAGTAATA TAATAAATGA ACAGTTCCTC 
AATTCCGAAC AAACAGATAC CATAGAAAAT AAAAATGACC TCCCAATTCA GTGGAATGAA 
GTAAGCTCTG GCAGTGCAGA ATATTCTCCA TCGAGATTAA AGTATGGGCA GCGTTTAACC 
ACACAGCAGA ATCGACCTTT TGGATTGTAT AATAACATGA TGGTCCAGCA GCAAAATGTA 
CCAAAGAACG GGTTGCCCCA GCAAAGGAGC TATCAACATC ATACACAAAA CAACCCACAG 
GTGCCCCAGC AAAATCTGAA CCTTTACAAT AACAGCAGTG TGTGGAGTGG TCAGTCAGTC 
AGGGGCAATC AGTGCATTGA CATAGCAAGA TCATCACTGG GAACGTCTGC AACAGGAAGT 
CTTTGCAATA CAACTCGAGG ACAAAAATTT ACAGCCAATG GTTTGCAAAT GAATATTGGT 
CAACAAAACT ATGGACCATC CACACATTAT AGTGTGCAAT CAGCTAGCAG AACTGACCCA 
GTCCAGAATG AAAACATCAT GAATCAGGAA TTTATGCACC AGATGACAGC CGAACACCTA 
ATTTATGGTA TGCATTTCCT GGGGGTAACT CGATCTAATC AGACAACATC AGGGCAAAAT 
GGAAACACAA TTGATGCAAC ACAAAGTTTC CTGTCAACTA CTCAAAATGC TGGAGAGCAG 
CAGCCATCCC TGGCAAAGAA TATCCAGTCT TTTGTAAACT CTAGTGGAAA TAGCAGGCAG 
AATATACATA GAAACAACCT GTCATATCTG CAAGAGCAAA TATGTGACAC CAACCAACAA 
GTTTGCAGAG TGAATGGTAT TAAAATGGAA ATGCAGAATC ACCCACAACA GCACTGTGCG 
AATGCACAAA ACTTCTCCGG TCAGTTGTAT GACCAAACAG CAGCATATCC TCAGCAAAGT 
ATCAAGTCAG GTTCTGAGCC TGGTATGGAA GCAAACTGTC TTTTACAAGA AAGTAATAAT 
ACAAACTCAT CCCAGCTTCT TTCGCCTGGG GCAAACCAAG TGACTAGCAC AGTCAATAAC 
ATTGACAGCA GTTTGGATGA GGTGCAAATC GATTTTGATG CTATAATTGA TGATGGGGAT 
CATGCCAGCC TCATTTCTGG AGCTCTAAGT CCAAGTATCA TCCAGAATCT CTCCCGTAAT 
TCTTCACGCC TTACCACTCC TCGAACATCT CTGACATTCC CAGCCATACC TGTTAGTACC 
AGTAACATGG CTATTGGGGA CATGAGCTCT TTGCTGACCT CACTTGCAGA AGAAAGCAAG 
TTTCTTGCTA TTATGCAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915407.1
CDS	405..5105
Translation	MEAQSRSTTASEKKKVENSIIKGHTRTEVSEKAVASSTTSNEDESPGQTYHRERRNAITMQPHGGQGLGKINEEPSTSSEERASLIKKELHGSITHLPEPSVPYRGTVFAMDPRNGYIDPHYHPPHLFPSFHPPVPIDARHHEGRYHYEPTPIPPLHVPSALASSPTYPDLPFIRISPHRNPASASDSPFSPPHPYISPYMDYIRSLHSSPSLSMISAARGLSPTDVPHAGVSPAEYYHQMALLTGQRSPYADIIPSPATAGAGANALHMEYLHAMESSRFSSPRVPLRPSRKRTLSISPLSDHSFDLQTMIRNSPNSLVTILNNSRSSSSASGSYGHLAASAISPALSFTYQPTPVSLQQMHQQLMSRQHSIGSAFGHSPPLLHPAPTFPSQRTIPGIPSVLNPVQVSIGPSEAAQQNKPTSESAVSSTGDPLHNKRSKVKPEEDLPSPGAVCIQDQPDGMTLVKEEGDKDESKQEPEVVYETNCHWEGCSREFDTQEQLVHHINNDHIHGEKKEFVCRWLDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCSKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDIHPRPPPPREPGSHSQSRSPGQHIQGTHGEHKDLSNTTSKHEECLQVKSVKTEKPMTSQPSPGGKSSCSSEQSPVSNYTNSGIELTLNSGGSIGDFSALDETPIMDSTISTATTGLGLHGRRIMTGTKWMEQIKVEKIKQVNGILPRLNPVPPHRAPNLPPITGNGVQLNSNISLGGSAPVLPNKNELSSMDITVLNMLNRRDSNTSTISSAYMSSRRSSGISPCFSSRRSSEASQFEGRVQNISVADSYDPISTDASRRSSETSQCDGLPSLLSLTPAQQYRLKAKYAAATGGPPPTPLPNMERMSLKTRIALLGDSRDCRLSPLPSVNVPRRCSDGGVGSYGRRHLLSNELQGNGMRRASDPVKMVSDNFSNARVPRFNSLNNITPPDAPPLMERRNLSLQNYTRNVYSPCPPSISENVALEAMIMEAEGNFDDEDLLPDDMVQYLNSRNQRLYDIFETDKLQNNAGERNGNNFEPSHVTSNIINEQFLNSEQTDTIENKNDLPIQWNEVSSGSAEYSPSRLKYGQRLTTQQNRPFGLYNNMMVQQQNVPKNGLPQQRSYQHHTQNNPQVPQQNLNLYNNSSVWSGQSVRGNQCIDIARSSLGTSATGSLCNTTRGQKFTANGLQMNIGQQNYGPSTHYSVQSASRTDPVQNENIMNQEFMHQMTAEHLIYGMHFLGVTRSNQTTSGQNGNTIDATQSFLSTTQNAGEQQPSLAKNIQSFVNSSGNSRQNIHRNNLSYLQEQICDTNQQVCRVNGIKMEMQNHPQQHCANAQNFSGQLYDQTAAYPQQSIKSGSEPGMEANCLLQESNNTNSSQLLSPGANQVTSTVNNIDSSLDEVQIDFDAIIDDGDHASLISGALSPSIIQNLSRNSSRLTTPRTSLTFPAIPVSTSNMAIGDMSSLLTSLAEESKFLAIMQ

Target ORF information:

RefSeq Version	XM_004915350.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis GLI family zinc finger 3 (gli3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915350.3

ORF Insert Sequence:

ATGGAAGCCC AGTCCCGTAG CACTACAGCA AGTGAAAAGA AAAAGGTTGA GAACTCCATA 
ATCAAAGGTC ACACTCGAAC AGAAGTCAGC GAGAAAGCTG TTGCCTCCAG CACAACTTCC 
AATGAGGATG AAAGTCCTGG ACAGACATAT CACAGAGAAA GAAGAAACGC CATCACCATG 
CAGCCTCATG GAGGCCAAGG TCTTGGTAAA ATCAATGAGG AACCCTCTAC GTCAAGTGAG 
GAAAGGGCCT CATTAATAAA AAAAGAACTT CATGGATCTA TAACACATCT GCCTGAACCC 
TCAGTTCCCT ACCGTGGCAC TGTCTTTGCC ATGGACCCTA GGAATGGTTA TATTGATCCA 
CATTACCATC CGCCTCATCT CTTCCCATCT TTTCATCCTC CTGTGCCAAT TGATGCAAGA 
CATCATGAGG GAAGATACCA TTATGAGCCA ACTCCCATTC CGCCACTGCA TGTGCCTTCT 
GCATTAGCAA GCAGCCCGAC ATACCCAGAT CTTCCATTTA TCCGGATATC TCCACACAGA 
AACCCAGCCA GTGCATCTGA TTCCCCATTC AGCCCTCCTC ATCCTTACAT CAGTCCTTAC 
ATGGACTATA TCAGATCTCT CCACAGCAGC CCTTCACTGT CTATGATATC AGCTGCCCGT 
GGACTCAGCC CTACTGATGT CCCACATGCT GGTGTTAGCC CTGCTGAATA TTACCATCAA 
ATGGCACTGC TAACTGGACA GAGAAGCCCA TATGCTGATA TTATTCCATC CCCTGCAACT 
GCTGGTGCTG GAGCTAATGC CCTCCATATG GAATATCTTC ATGCCATGGA GAGCTCACGG 
TTTTCAAGTC CTAGAGTGCC ATTACGACCA AGTCGCAAGA GAACACTGTC CATATCACCT 
CTTTCTGATC ATAGCTTTGA TCTCCAGACA ATGATTCGCA ACTCTCCAAA TTCTCTTGTT 
ACTATACTTA ACAACTCCCG CAGCAGCTCA TCTGCTAGTG GCTCTTATGG GCATCTAGCA 
GCCAGTGCCA TCAGTCCAGC ATTGAGTTTT ACATACCAGC CTACACCAGT GTCTCTTCAG 
CAAATGCATC AACAGCTTAT GAGTCGACAG CACAGCATAG GCTCAGCTTT TGGACACAGC 
CCTCCACTTC TTCACCCAGC ACCAACATTT CCTTCTCAAA GGACCATTCC CGGCATCCCC 
AGTGTTTTGA ATCCAGTGCA GGTCAGCATT GGGCCATCAG AGGCTGCACA GCAAAACAAG 
CCCACCAGTG AGTCAGCAGT CAGCAGCACA GGTGACCCCC TGCACAACAA GCGTTCTAAG 
GTAAAACCTG AAGAAGATCT TCCAAGCCCT GGAGCAGTGT GTATTCAGGA TCAGCCAGAT 
GGAATGACTC TGGTGAAAGA GGAGGGGGAC AAAGATGAAA GTAAGCAAGA GCCTGAAGTG 
GTCTACGAGA CAAACTGCCA TTGGGAAGGC TGCTCGCGAG AGTTTGACAC ACAGGAACAG 
CTAGTGCATC ATATCAACAA TGACCACATA CATGGAGAGA AAAAGGAGTT TGTGTGCCGA 
TGGCTAGATT GTTCAAGAGA ACAAAAGCCG TTTAAGGCTC AGTACATGCT GGTGGTCCAC 
ATGAGAAGAC ATACAGGAGA AAAGCCACAC AAATGCACTT TTGAAGGTTG TTCAAAAGCC 
TACTCCAGAC TAGAAAACTT GAAAACTCAC CTGAGATCTC ACACTGGAGA GAAACCATAT 
GTCTGTGAGC ATGAGGGCTG CAACAAGGCC TTTTCAAATG CATCTGATAG AGCCAAGCAC 
CAAAACCGCA CTCATTCAAA TGAGAAACCT TATGTTTGCA AGATCCCAGG GTGTACAAAA 
CGTTACACTG ACCCTAGCTC ACTTAGAAAG CATGTAAAGA CAGTGCATGG TCCAGAGGCT 
CATGTAACTA AGAAGCAGAG AGGGGACATA CACCCAAGGC CTCCACCACC ACGAGAACCA 
GGAAGCCATT CGCAGTCAAG ATCACCAGGC CAACATATCC AGGGTACACA TGGAGAGCAC 
AAGGACCTCA GTAACACTAC CTCAAAGCAT GAAGAATGCC TGCAAGTAAA ATCAGTCAAG 
ACTGAAAAGC CAATGACATC TCAGCCAAGC CCTGGTGGTA AGTCTTCATG CAGCAGCGAG 
CAGTCCCCCG TCAGCAACTA TACCAACAGT GGGATCGAGC TTACTCTAAA CAGTGGAGGC 
AGCATTGGAG ACTTCAGTGC CCTAGATGAA ACCCCAATCA TGGACTCTAC CATTTCCACC 
GCAACCACAG GACTTGGTTT ACACGGAAGG AGGATTATGA CAGGAACCAA ATGGATGGAG 
CAAATTAAAG TTGAAAAAAT TAAACAAGTT AATGGTATTC TTCCAAGACT GAACCCAGTT 
CCACCTCATA GAGCACCAAA TTTACCACCA ATCACTGGAA ATGGTGTGCA GCTGAACAGC 
AACATCAGTT TGGGGGGATC AGCACCTGTT CTTCCAAACA AAAATGAGCT TTCTAGCATG 
GACATTACAG TCTTGAATAT GCTTAACAGA AGAGATAGCA ATACTAGCAC AATAAGTTCA 
GCATATATGA GCAGCCGCAG GTCCTCTGGA ATTTCACCTT GTTTTTCCAG TCGAAGATCC 
AGTGAGGCAT CTCAGTTTGA GGGACGGGTT CAGAATATAA GTGTTGCAGA CTCCTATGAT 
CCAATTTCTA CTGATGCCTC AAGACGATCA AGTGAAACAA GCCAGTGTGA TGGGCTGCCA 
AGCCTCCTAA GCCTTACTCC AGCACAGCAA TACAGGCTGA AAGCTAAATA TGCTGCTGCT 
ACAGGTGGGC CCCCACCAAC ACCACTGCCG AACATGGAAA GAATGAGTCT AAAAACCAGA 
ATAGCTCTCC TGGGTGACTC AAGAGATTGT AGATTGTCAC CTCTTCCTTC AGTCAATGTC 
CCACGAAGAT GTAGTGATGG TGGAGTAGGC TCTTATGGAA GAAGGCATTT GTTGTCAAAT 
GAACTTCAAG GTAATGGAAT GCGTAGAGCA AGTGATCCTG TAAAAATGGT CTCTGATAAC 
TTTTCTAATG CTAGAGTTCC AAGGTTCAAC AGTCTTAACA ACATCACCCC TCCTGATGCC 
CCGCCACTTA TGGAAAGGCG CAATTTAAGT CTTCAGAACT ATACCCGTAA TGTGTATTCA 
CCATGCCCTC CTAGTATAAG TGAAAATGTT GCCCTGGAAG CAATGATAAT GGAAGCAGAA 
GGCAATTTTG ATGATGAGGA TTTACTGCCA GATGACATGG TTCAGTATCT TAACTCTCGG 
AACCAAAGAC TTTATGACAT TTTTGAAACA GATAAATTAC AGAATAATGC TGGAGAAAGA 
AATGGAAACA ACTTTGAACC ATCTCATGTA ACCAGTAATA TAATAAATGA ACAGTTCCTC 
AATTCCGAAC AAACAGATAC CATAGAAAAT AAAAATGACC TCCCAATTCA GTGGAATGAA 
GTAAGCTCTG GCAGTGCAGA ATATTCTCCA TCGAGATTAA AGTATGGGCA GCGTTTAACC 
ACACAGCAGA ATCGACCTTT TGGATTGTAT AATAACATGA TGGTCCAGCA GCAAAATGTA 
CCAAAGAACG GGTTGCCCCA GCAAAGGAGC TATCAACATC ATACACAAAA CAACCCACAG 
GTGCCCCAGC AAAATCTGAA CCTTTACAAT AACAGCAGTG TGTGGAGTGG TCAGTCAGTC 
AGGGGCAATC AGTGCATTGA CATAGCAAGA TCATCACTGG GAACGTCTGC AACAGGAAGT 
CTTTGCAATA CAACTCGAGG ACAAAAATTT ACAGCCAATG GTTTGCAAAT GAATATTGGT 
CAACAAAACT ATGGACCATC CACACATTAT AGTGTGCAAT CAGCTAGCAG AACTGACCCA 
GTCCAGAATG AAAACATCAT GAATCAGGAA TTTATGCACC AGATGACAGC CGAACACCTA 
ATTTATGGTA TGCATTTCCT GGGGGTAACT CGATCTAATC AGACAACATC AGGGCAAAAT 
GGAAACACAA TTGATGCAAC ACAAAGTTTC CTGTCAACTA CTCAAAATGC TGGAGAGCAG 
CAGCCATCCC TGGCAAAGAA TATCCAGTCT TTTGTAAACT CTAGTGGAAA TAGCAGGCAG 
AATATACATA GAAACAACCT GTCATATCTG CAAGAGCAAA TATGTGACAC CAACCAACAA 
GTTTGCAGAG TGAATGGTAT TAAAATGGAA ATGCAGAATC ACCCACAACA GCACTGTGCG 
AATGCACAAA ACTTCTCCGG TCAGTTGTAT GACCAAACAG CAGCATATCC TCAGCAAAGT 
ATCAAGTCAG GTTCTGAGCC TGGTATGGAA GCAAACTGTC TTTTACAAGA AAGTAATAAT 
ACAAACTCAT CCCAGCTTCT TTCGCCTGGG GCAAACCAAG TGACTAGCAC AGTCAATAAC 
ATTGACAGCA GTTTGGATGA GGTGCAAATC GATTTTGATG CTATAATTGA TGATGGGGAT 
CATGCCAGCC TCATTTCTGG AGCTCTAAGT CCAAGTATCA TCCAGAATCT CTCCCGTAAT 
TCTTCACGCC TTACCACTCC TCGAACATCT CTGACATTCC CAGCCATACC TGTTAGTACC 
AGTAACATGG CTATTGGGGA CATGAGCTCT TTGCTGACCT CACTTGCAGA AGAAAGCAAG 
TTTCTTGCTA TTATGCAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gli3 ( XM_004915351.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915408.1 Xenopus tropicalis GLI family zinc finger 3 (gli3), transcript variant X1, mRNA.

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CloneID	OXa11836
Clone ID Related Accession (Same CDS sequence)	XM_004915350.3 , XM_004915350.4 , XM_004915351.4 , XM_004915351.3
Accession Version	XM_004915351.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transcriptional activator GLI3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004915351.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCCC AGTCCCGTAG CACTACAGCA AGTGAAAAGA AAAAGGTTGA GAACTCCATA 
ATCAAAGGTC ACACTCGAAC AGAAGTCAGC GAGAAAGCTG TTGCCTCCAG CACAACTTCC 
AATGAGGATG AAAGTCCTGG ACAGACATAT CACAGAGAAA GAAGAAACGC CATCACCATG 
CAGCCTCATG GAGGCCAAGG TCTTGGTAAA ATCAATGAGG AACCCTCTAC GTCAAGTGAG 
GAAAGGGCCT CATTAATAAA AAAAGAACTT CATGGATCTA TAACACATCT GCCTGAACCC 
TCAGTTCCCT ACCGTGGCAC TGTCTTTGCC ATGGACCCTA GGAATGGTTA TATTGATCCA 
CATTACCATC CGCCTCATCT CTTCCCATCT TTTCATCCTC CTGTGCCAAT TGATGCAAGA 
CATCATGAGG GAAGATACCA TTATGAGCCA ACTCCCATTC CGCCACTGCA TGTGCCTTCT 
GCATTAGCAA GCAGCCCGAC ATACCCAGAT CTTCCATTTA TCCGGATATC TCCACACAGA 
AACCCAGCCA GTGCATCTGA TTCCCCATTC AGCCCTCCTC ATCCTTACAT CAGTCCTTAC 
ATGGACTATA TCAGATCTCT CCACAGCAGC CCTTCACTGT CTATGATATC AGCTGCCCGT 
GGACTCAGCC CTACTGATGT CCCACATGCT GGTGTTAGCC CTGCTGAATA TTACCATCAA 
ATGGCACTGC TAACTGGACA GAGAAGCCCA TATGCTGATA TTATTCCATC CCCTGCAACT 
GCTGGTGCTG GAGCTAATGC CCTCCATATG GAATATCTTC ATGCCATGGA GAGCTCACGG 
TTTTCAAGTC CTAGAGTGCC ATTACGACCA AGTCGCAAGA GAACACTGTC CATATCACCT 
CTTTCTGATC ATAGCTTTGA TCTCCAGACA ATGATTCGCA ACTCTCCAAA TTCTCTTGTT 
ACTATACTTA ACAACTCCCG CAGCAGCTCA TCTGCTAGTG GCTCTTATGG GCATCTAGCA 
GCCAGTGCCA TCAGTCCAGC ATTGAGTTTT ACATACCAGC CTACACCAGT GTCTCTTCAG 
CAAATGCATC AACAGCTTAT GAGTCGACAG CACAGCATAG GCTCAGCTTT TGGACACAGC 
CCTCCACTTC TTCACCCAGC ACCAACATTT CCTTCTCAAA GGACCATTCC CGGCATCCCC 
AGTGTTTTGA ATCCAGTGCA GGTCAGCATT GGGCCATCAG AGGCTGCACA GCAAAACAAG 
CCCACCAGTG AGTCAGCAGT CAGCAGCACA GGTGACCCCC TGCACAACAA GCGTTCTAAG 
GTAAAACCTG AAGAAGATCT TCCAAGCCCT GGAGCAGTGT GTATTCAGGA TCAGCCAGAT 
GGAATGACTC TGGTGAAAGA GGAGGGGGAC AAAGATGAAA GTAAGCAAGA GCCTGAAGTG 
GTCTACGAGA CAAACTGCCA TTGGGAAGGC TGCTCGCGAG AGTTTGACAC ACAGGAACAG 
CTAGTGCATC ATATCAACAA TGACCACATA CATGGAGAGA AAAAGGAGTT TGTGTGCCGA 
TGGCTAGATT GTTCAAGAGA ACAAAAGCCG TTTAAGGCTC AGTACATGCT GGTGGTCCAC 
ATGAGAAGAC ATACAGGAGA AAAGCCACAC AAATGCACTT TTGAAGGTTG TTCAAAAGCC 
TACTCCAGAC TAGAAAACTT GAAAACTCAC CTGAGATCTC ACACTGGAGA GAAACCATAT 
GTCTGTGAGC ATGAGGGCTG CAACAAGGCC TTTTCAAATG CATCTGATAG AGCCAAGCAC 
CAAAACCGCA CTCATTCAAA TGAGAAACCT TATGTTTGCA AGATCCCAGG GTGTACAAAA 
CGTTACACTG ACCCTAGCTC ACTTAGAAAG CATGTAAAGA CAGTGCATGG TCCAGAGGCT 
CATGTAACTA AGAAGCAGAG AGGGGACATA CACCCAAGGC CTCCACCACC ACGAGAACCA 
GGAAGCCATT CGCAGTCAAG ATCACCAGGC CAACATATCC AGGGTACACA TGGAGAGCAC 
AAGGACCTCA GTAACACTAC CTCAAAGCAT GAAGAATGCC TGCAAGTAAA ATCAGTCAAG 
ACTGAAAAGC CAATGACATC TCAGCCAAGC CCTGGTGGTA AGTCTTCATG CAGCAGCGAG 
CAGTCCCCCG TCAGCAACTA TACCAACAGT GGGATCGAGC TTACTCTAAA CAGTGGAGGC 
AGCATTGGAG ACTTCAGTGC CCTAGATGAA ACCCCAATCA TGGACTCTAC CATTTCCACC 
GCAACCACAG GACTTGGTTT ACACGGAAGG AGGATTATGA CAGGAACCAA ATGGATGGAG 
CAAATTAAAG TTGAAAAAAT TAAACAAGTT AATGGTATTC TTCCAAGACT GAACCCAGTT 
CCACCTCATA GAGCACCAAA TTTACCACCA ATCACTGGAA ATGGTGTGCA GCTGAACAGC 
AACATCAGTT TGGGGGGATC AGCACCTGTT CTTCCAAACA AAAATGAGCT TTCTAGCATG 
GACATTACAG TCTTGAATAT GCTTAACAGA AGAGATAGCA ATACTAGCAC AATAAGTTCA 
GCATATATGA GCAGCCGCAG GTCCTCTGGA ATTTCACCTT GTTTTTCCAG TCGAAGATCC 
AGTGAGGCAT CTCAGTTTGA GGGACGGGTT CAGAATATAA GTGTTGCAGA CTCCTATGAT 
CCAATTTCTA CTGATGCCTC AAGACGATCA AGTGAAACAA GCCAGTGTGA TGGGCTGCCA 
AGCCTCCTAA GCCTTACTCC AGCACAGCAA TACAGGCTGA AAGCTAAATA TGCTGCTGCT 
ACAGGTGGGC CCCCACCAAC ACCACTGCCG AACATGGAAA GAATGAGTCT AAAAACCAGA 
ATAGCTCTCC TGGGTGACTC AAGAGATTGT AGATTGTCAC CTCTTCCTTC AGTCAATGTC 
CCACGAAGAT GTAGTGATGG TGGAGTAGGC TCTTATGGAA GAAGGCATTT GTTGTCAAAT 
GAACTTCAAG GTAATGGAAT GCGTAGAGCA AGTGATCCTG TAAAAATGGT CTCTGATAAC 
TTTTCTAATG CTAGAGTTCC AAGGTTCAAC AGTCTTAACA ACATCACCCC TCCTGATGCC 
CCGCCACTTA TGGAAAGGCG CAATTTAAGT CTTCAGAACT ATACCCGTAA TGTGTATTCA 
CCATGCCCTC CTAGTATAAG TGAAAATGTT GCCCTGGAAG CAATGATAAT GGAAGCAGAA 
GGCAATTTTG ATGATGAGGA TTTACTGCCA GATGACATGG TTCAGTATCT TAACTCTCGG 
AACCAAAGAC TTTATGACAT TTTTGAAACA GATAAATTAC AGAATAATGC TGGAGAAAGA 
AATGGAAACA ACTTTGAACC ATCTCATGTA ACCAGTAATA TAATAAATGA ACAGTTCCTC 
AATTCCGAAC AAACAGATAC CATAGAAAAT AAAAATGACC TCCCAATTCA GTGGAATGAA 
GTAAGCTCTG GCAGTGCAGA ATATTCTCCA TCGAGATTAA AGTATGGGCA GCGTTTAACC 
ACACAGCAGA ATCGACCTTT TGGATTGTAT AATAACATGA TGGTCCAGCA GCAAAATGTA 
CCAAAGAACG GGTTGCCCCA GCAAAGGAGC TATCAACATC ATACACAAAA CAACCCACAG 
GTGCCCCAGC AAAATCTGAA CCTTTACAAT AACAGCAGTG TGTGGAGTGG TCAGTCAGTC 
AGGGGCAATC AGTGCATTGA CATAGCAAGA TCATCACTGG GAACGTCTGC AACAGGAAGT 
CTTTGCAATA CAACTCGAGG ACAAAAATTT ACAGCCAATG GTTTGCAAAT GAATATTGGT 
CAACAAAACT ATGGACCATC CACACATTAT AGTGTGCAAT CAGCTAGCAG AACTGACCCA 
GTCCAGAATG AAAACATCAT GAATCAGGAA TTTATGCACC AGATGACAGC CGAACACCTA 
ATTTATGGTA TGCATTTCCT GGGGGTAACT CGATCTAATC AGACAACATC AGGGCAAAAT 
GGAAACACAA TTGATGCAAC ACAAAGTTTC CTGTCAACTA CTCAAAATGC TGGAGAGCAG 
CAGCCATCCC TGGCAAAGAA TATCCAGTCT TTTGTAAACT CTAGTGGAAA TAGCAGGCAG 
AATATACATA GAAACAACCT GTCATATCTG CAAGAGCAAA TATGTGACAC CAACCAACAA 
GTTTGCAGAG TGAATGGTAT TAAAATGGAA ATGCAGAATC ACCCACAACA GCACTGTGCG 
AATGCACAAA ACTTCTCCGG TCAGTTGTAT GACCAAACAG CAGCATATCC TCAGCAAAGT 
ATCAAGTCAG GTTCTGAGCC TGGTATGGAA GCAAACTGTC TTTTACAAGA AAGTAATAAT 
ACAAACTCAT CCCAGCTTCT TTCGCCTGGG GCAAACCAAG TGACTAGCAC AGTCAATAAC 
ATTGACAGCA GTTTGGATGA GGTGCAAATC GATTTTGATG CTATAATTGA TGATGGGGAT 
CATGCCAGCC TCATTTCTGG AGCTCTAAGT CCAAGTATCA TCCAGAATCT CTCCCGTAAT 
TCTTCACGCC TTACCACTCC TCGAACATCT CTGACATTCC CAGCCATACC TGTTAGTACC 
AGTAACATGG CTATTGGGGA CATGAGCTCT TTGCTGACCT CACTTGCAGA AGAAAGCAAG 
TTTCTTGCTA TTATGCAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915408.1
CDS	873..5573
Translation	MEAQSRSTTASEKKKVENSIIKGHTRTEVSEKAVASSTTSNEDESPGQTYHRERRNAITMQPHGGQGLGKINEEPSTSSEERASLIKKELHGSITHLPEPSVPYRGTVFAMDPRNGYIDPHYHPPHLFPSFHPPVPIDARHHEGRYHYEPTPIPPLHVPSALASSPTYPDLPFIRISPHRNPASASDSPFSPPHPYISPYMDYIRSLHSSPSLSMISAARGLSPTDVPHAGVSPAEYYHQMALLTGQRSPYADIIPSPATAGAGANALHMEYLHAMESSRFSSPRVPLRPSRKRTLSISPLSDHSFDLQTMIRNSPNSLVTILNNSRSSSSASGSYGHLAASAISPALSFTYQPTPVSLQQMHQQLMSRQHSIGSAFGHSPPLLHPAPTFPSQRTIPGIPSVLNPVQVSIGPSEAAQQNKPTSESAVSSTGDPLHNKRSKVKPEEDLPSPGAVCIQDQPDGMTLVKEEGDKDESKQEPEVVYETNCHWEGCSREFDTQEQLVHHINNDHIHGEKKEFVCRWLDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCSKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDIHPRPPPPREPGSHSQSRSPGQHIQGTHGEHKDLSNTTSKHEECLQVKSVKTEKPMTSQPSPGGKSSCSSEQSPVSNYTNSGIELTLNSGGSIGDFSALDETPIMDSTISTATTGLGLHGRRIMTGTKWMEQIKVEKIKQVNGILPRLNPVPPHRAPNLPPITGNGVQLNSNISLGGSAPVLPNKNELSSMDITVLNMLNRRDSNTSTISSAYMSSRRSSGISPCFSSRRSSEASQFEGRVQNISVADSYDPISTDASRRSSETSQCDGLPSLLSLTPAQQYRLKAKYAAATGGPPPTPLPNMERMSLKTRIALLGDSRDCRLSPLPSVNVPRRCSDGGVGSYGRRHLLSNELQGNGMRRASDPVKMVSDNFSNARVPRFNSLNNITPPDAPPLMERRNLSLQNYTRNVYSPCPPSISENVALEAMIMEAEGNFDDEDLLPDDMVQYLNSRNQRLYDIFETDKLQNNAGERNGNNFEPSHVTSNIINEQFLNSEQTDTIENKNDLPIQWNEVSSGSAEYSPSRLKYGQRLTTQQNRPFGLYNNMMVQQQNVPKNGLPQQRSYQHHTQNNPQVPQQNLNLYNNSSVWSGQSVRGNQCIDIARSSLGTSATGSLCNTTRGQKFTANGLQMNIGQQNYGPSTHYSVQSASRTDPVQNENIMNQEFMHQMTAEHLIYGMHFLGVTRSNQTTSGQNGNTIDATQSFLSTTQNAGEQQPSLAKNIQSFVNSSGNSRQNIHRNNLSYLQEQICDTNQQVCRVNGIKMEMQNHPQQHCANAQNFSGQLYDQTAAYPQQSIKSGSEPGMEANCLLQESNNTNSSQLLSPGANQVTSTVNNIDSSLDEVQIDFDAIIDDGDHASLISGALSPSIIQNLSRNSSRLTTPRTSLTFPAIPVSTSNMAIGDMSSLLTSLAEESKFLAIMQ

Target ORF information:

RefSeq Version	XM_004915351.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis GLI family zinc finger 3 (gli3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915351.3

ORF Insert Sequence:

ATGGAAGCCC AGTCCCGTAG CACTACAGCA AGTGAAAAGA AAAAGGTTGA GAACTCCATA 
ATCAAAGGTC ACACTCGAAC AGAAGTCAGC GAGAAAGCTG TTGCCTCCAG CACAACTTCC 
AATGAGGATG AAAGTCCTGG ACAGACATAT CACAGAGAAA GAAGAAACGC CATCACCATG 
CAGCCTCATG GAGGCCAAGG TCTTGGTAAA ATCAATGAGG AACCCTCTAC GTCAAGTGAG 
GAAAGGGCCT CATTAATAAA AAAAGAACTT CATGGATCTA TAACACATCT GCCTGAACCC 
TCAGTTCCCT ACCGTGGCAC TGTCTTTGCC ATGGACCCTA GGAATGGTTA TATTGATCCA 
CATTACCATC CGCCTCATCT CTTCCCATCT TTTCATCCTC CTGTGCCAAT TGATGCAAGA 
CATCATGAGG GAAGATACCA TTATGAGCCA ACTCCCATTC CGCCACTGCA TGTGCCTTCT 
GCATTAGCAA GCAGCCCGAC ATACCCAGAT CTTCCATTTA TCCGGATATC TCCACACAGA 
AACCCAGCCA GTGCATCTGA TTCCCCATTC AGCCCTCCTC ATCCTTACAT CAGTCCTTAC 
ATGGACTATA TCAGATCTCT CCACAGCAGC CCTTCACTGT CTATGATATC AGCTGCCCGT 
GGACTCAGCC CTACTGATGT CCCACATGCT GGTGTTAGCC CTGCTGAATA TTACCATCAA 
ATGGCACTGC TAACTGGACA GAGAAGCCCA TATGCTGATA TTATTCCATC CCCTGCAACT 
GCTGGTGCTG GAGCTAATGC CCTCCATATG GAATATCTTC ATGCCATGGA GAGCTCACGG 
TTTTCAAGTC CTAGAGTGCC ATTACGACCA AGTCGCAAGA GAACACTGTC CATATCACCT 
CTTTCTGATC ATAGCTTTGA TCTCCAGACA ATGATTCGCA ACTCTCCAAA TTCTCTTGTT 
ACTATACTTA ACAACTCCCG CAGCAGCTCA TCTGCTAGTG GCTCTTATGG GCATCTAGCA 
GCCAGTGCCA TCAGTCCAGC ATTGAGTTTT ACATACCAGC CTACACCAGT GTCTCTTCAG 
CAAATGCATC AACAGCTTAT GAGTCGACAG CACAGCATAG GCTCAGCTTT TGGACACAGC 
CCTCCACTTC TTCACCCAGC ACCAACATTT CCTTCTCAAA GGACCATTCC CGGCATCCCC 
AGTGTTTTGA ATCCAGTGCA GGTCAGCATT GGGCCATCAG AGGCTGCACA GCAAAACAAG 
CCCACCAGTG AGTCAGCAGT CAGCAGCACA GGTGACCCCC TGCACAACAA GCGTTCTAAG 
GTAAAACCTG AAGAAGATCT TCCAAGCCCT GGAGCAGTGT GTATTCAGGA TCAGCCAGAT 
GGAATGACTC TGGTGAAAGA GGAGGGGGAC AAAGATGAAA GTAAGCAAGA GCCTGAAGTG 
GTCTACGAGA CAAACTGCCA TTGGGAAGGC TGCTCGCGAG AGTTTGACAC ACAGGAACAG 
CTAGTGCATC ATATCAACAA TGACCACATA CATGGAGAGA AAAAGGAGTT TGTGTGCCGA 
TGGCTAGATT GTTCAAGAGA ACAAAAGCCG TTTAAGGCTC AGTACATGCT GGTGGTCCAC 
ATGAGAAGAC ATACAGGAGA AAAGCCACAC AAATGCACTT TTGAAGGTTG TTCAAAAGCC 
TACTCCAGAC TAGAAAACTT GAAAACTCAC CTGAGATCTC ACACTGGAGA GAAACCATAT 
GTCTGTGAGC ATGAGGGCTG CAACAAGGCC TTTTCAAATG CATCTGATAG AGCCAAGCAC 
CAAAACCGCA CTCATTCAAA TGAGAAACCT TATGTTTGCA AGATCCCAGG GTGTACAAAA 
CGTTACACTG ACCCTAGCTC ACTTAGAAAG CATGTAAAGA CAGTGCATGG TCCAGAGGCT 
CATGTAACTA AGAAGCAGAG AGGGGACATA CACCCAAGGC CTCCACCACC ACGAGAACCA 
GGAAGCCATT CGCAGTCAAG ATCACCAGGC CAACATATCC AGGGTACACA TGGAGAGCAC 
AAGGACCTCA GTAACACTAC CTCAAAGCAT GAAGAATGCC TGCAAGTAAA ATCAGTCAAG 
ACTGAAAAGC CAATGACATC TCAGCCAAGC CCTGGTGGTA AGTCTTCATG CAGCAGCGAG 
CAGTCCCCCG TCAGCAACTA TACCAACAGT GGGATCGAGC TTACTCTAAA CAGTGGAGGC 
AGCATTGGAG ACTTCAGTGC CCTAGATGAA ACCCCAATCA TGGACTCTAC CATTTCCACC 
GCAACCACAG GACTTGGTTT ACACGGAAGG AGGATTATGA CAGGAACCAA ATGGATGGAG 
CAAATTAAAG TTGAAAAAAT TAAACAAGTT AATGGTATTC TTCCAAGACT GAACCCAGTT 
CCACCTCATA GAGCACCAAA TTTACCACCA ATCACTGGAA ATGGTGTGCA GCTGAACAGC 
AACATCAGTT TGGGGGGATC AGCACCTGTT CTTCCAAACA AAAATGAGCT TTCTAGCATG 
GACATTACAG TCTTGAATAT GCTTAACAGA AGAGATAGCA ATACTAGCAC AATAAGTTCA 
GCATATATGA GCAGCCGCAG GTCCTCTGGA ATTTCACCTT GTTTTTCCAG TCGAAGATCC 
AGTGAGGCAT CTCAGTTTGA GGGACGGGTT CAGAATATAA GTGTTGCAGA CTCCTATGAT 
CCAATTTCTA CTGATGCCTC AAGACGATCA AGTGAAACAA GCCAGTGTGA TGGGCTGCCA 
AGCCTCCTAA GCCTTACTCC AGCACAGCAA TACAGGCTGA AAGCTAAATA TGCTGCTGCT 
ACAGGTGGGC CCCCACCAAC ACCACTGCCG AACATGGAAA GAATGAGTCT AAAAACCAGA 
ATAGCTCTCC TGGGTGACTC AAGAGATTGT AGATTGTCAC CTCTTCCTTC AGTCAATGTC 
CCACGAAGAT GTAGTGATGG TGGAGTAGGC TCTTATGGAA GAAGGCATTT GTTGTCAAAT 
GAACTTCAAG GTAATGGAAT GCGTAGAGCA AGTGATCCTG TAAAAATGGT CTCTGATAAC 
TTTTCTAATG CTAGAGTTCC AAGGTTCAAC AGTCTTAACA ACATCACCCC TCCTGATGCC 
CCGCCACTTA TGGAAAGGCG CAATTTAAGT CTTCAGAACT ATACCCGTAA TGTGTATTCA 
CCATGCCCTC CTAGTATAAG TGAAAATGTT GCCCTGGAAG CAATGATAAT GGAAGCAGAA 
GGCAATTTTG ATGATGAGGA TTTACTGCCA GATGACATGG TTCAGTATCT TAACTCTCGG 
AACCAAAGAC TTTATGACAT TTTTGAAACA GATAAATTAC AGAATAATGC TGGAGAAAGA 
AATGGAAACA ACTTTGAACC ATCTCATGTA ACCAGTAATA TAATAAATGA ACAGTTCCTC 
AATTCCGAAC AAACAGATAC CATAGAAAAT AAAAATGACC TCCCAATTCA GTGGAATGAA 
GTAAGCTCTG GCAGTGCAGA ATATTCTCCA TCGAGATTAA AGTATGGGCA GCGTTTAACC 
ACACAGCAGA ATCGACCTTT TGGATTGTAT AATAACATGA TGGTCCAGCA GCAAAATGTA 
CCAAAGAACG GGTTGCCCCA GCAAAGGAGC TATCAACATC ATACACAAAA CAACCCACAG 
GTGCCCCAGC AAAATCTGAA CCTTTACAAT AACAGCAGTG TGTGGAGTGG TCAGTCAGTC 
AGGGGCAATC AGTGCATTGA CATAGCAAGA TCATCACTGG GAACGTCTGC AACAGGAAGT 
CTTTGCAATA CAACTCGAGG ACAAAAATTT ACAGCCAATG GTTTGCAAAT GAATATTGGT 
CAACAAAACT ATGGACCATC CACACATTAT AGTGTGCAAT CAGCTAGCAG AACTGACCCA 
GTCCAGAATG AAAACATCAT GAATCAGGAA TTTATGCACC AGATGACAGC CGAACACCTA 
ATTTATGGTA TGCATTTCCT GGGGGTAACT CGATCTAATC AGACAACATC AGGGCAAAAT 
GGAAACACAA TTGATGCAAC ACAAAGTTTC CTGTCAACTA CTCAAAATGC TGGAGAGCAG 
CAGCCATCCC TGGCAAAGAA TATCCAGTCT TTTGTAAACT CTAGTGGAAA TAGCAGGCAG 
AATATACATA GAAACAACCT GTCATATCTG CAAGAGCAAA TATGTGACAC CAACCAACAA 
GTTTGCAGAG TGAATGGTAT TAAAATGGAA ATGCAGAATC ACCCACAACA GCACTGTGCG 
AATGCACAAA ACTTCTCCGG TCAGTTGTAT GACCAAACAG CAGCATATCC TCAGCAAAGT 
ATCAAGTCAG GTTCTGAGCC TGGTATGGAA GCAAACTGTC TTTTACAAGA AAGTAATAAT 
ACAAACTCAT CCCAGCTTCT TTCGCCTGGG GCAAACCAAG TGACTAGCAC AGTCAATAAC 
ATTGACAGCA GTTTGGATGA GGTGCAAATC GATTTTGATG CTATAATTGA TGATGGGGAT 
CATGCCAGCC TCATTTCTGG AGCTCTAAGT CCAAGTATCA TCCAGAATCT CTCCCGTAAT 
TCTTCACGCC TTACCACTCC TCGAACATCT CTGACATTCC CAGCCATACC TGTTAGTACC 
AGTAACATGG CTATTGGGGA CATGAGCTCT TTGCTGACCT CACTTGCAGA AGAAAGCAAG 
TTTCTTGCTA TTATGCAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gli3 ( XM_012965492.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012820946.1 Xenopus tropicalis GLI family zinc finger 3 (gli3), transcript variant X3, mRNA.

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CloneID	OXa30279
Clone ID Related Accession (Same CDS sequence)	XM_012965492.2 , XM_012965492.3
Accession Version	XM_012965492.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transcriptional activator GLI3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965492.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCTC ATGGAGGCCA AGGTCTTGGT AAAATCAATG AGGAACCCTC TACGTCAAGT 
GAGGAAAGGG CCTCATTAAT AAAAAAAGAA CTTCATGGAT CTATAACACA TCTGCCTGAA 
CCCTCAGTTC CCTACCGTGG CACTGTCTTT GCCATGGACC CTAGGAATGG TTATATTGAT 
CCACATTACC ATCCGCCTCA TCTCTTCCCA TCTTTTCATC CTCCTGTGCC AATTGATGCA 
AGACATCATG AGGGAAGATA CCATTATGAG CCAACTCCCA TTCCGCCACT GCATGTGCCT 
TCTGCATTAG CAAGCAGCCC GACATACCCA GATCTTCCAT TTATCCGGAT ATCTCCACAC 
AGAAACCCAG CCAGTGCATC TGATTCCCCA TTCAGCCCTC CTCATCCTTA CATCAGTCCT 
TACATGGACT ATATCAGATC TCTCCACAGC AGCCCTTCAC TGTCTATGAT ATCAGCTGCC 
CGTGGACTCA GCCCTACTGA TGTCCCACAT GCTGGTGTTA GCCCTGCTGA ATATTACCAT 
CAAATGGCAC TGCTAACTGG ACAGAGAAGC CCATATGCTG ATATTATTCC ATCCCCTGCA 
ACTGCTGGTG CTGGAGCTAA TGCCCTCCAT ATGGAATATC TTCATGCCAT GGAGAGCTCA 
CGGTTTTCAA GTCCTAGAGT GCCATTACGA CCAAGTCGCA AGAGAACACT GTCCATATCA 
CCTCTTTCTG ATCATAGCTT TGATCTCCAG ACAATGATTC GCAACTCTCC AAATTCTCTT 
GTTACTATAC TTAACAACTC CCGCAGCAGC TCATCTGCTA GTGGCTCTTA TGGGCATCTA 
GCAGCCAGTG CCATCAGTCC AGCATTGAGT TTTACATACC AGCCTACACC AGTGTCTCTT 
CAGCAAATGC ATCAACAGCT TATGAGTCGA CAGCACAGCA TAGGCTCAGC TTTTGGACAC 
AGCCCTCCAC TTCTTCACCC AGCACCAACA TTTCCTTCTC AAAGGACCAT TCCCGGCATC 
CCCAGTGTTT TGAATCCAGT GCAGGTCAGC ATTGGGCCAT CAGAGGCTGC ACAGCAAAAC 
AAGCCCACCA GTGAGTCAGC AGTCAGCAGC ACAGGTGACC CCCTGCACAA CAAGCGTTCT 
AAGGTAAAAC CTGAAGAAGA TCTTCCAAGC CCTGGAGCAG TGTGTATTCA GGATCAGCCA 
GATGGAATGA CTCTGGTGAA AGAGGAGGGG GACAAAGATG AAAGTAAGCA AGAGCCTGAA 
GTGGTCTACG AGACAAACTG CCATTGGGAA GGCTGCTCGC GAGAGTTTGA CACACAGGAA 
CAGCTAGTGC ATCATATCAA CAATGACCAC ATACATGGAG AGAAAAAGGA GTTTGTGTGC 
CGATGGCTAG ATTGTTCAAG AGAACAAAAG CCGTTTAAGG CTCAGTACAT GCTGGTGGTC 
CACATGAGAA GACATACAGG AGAAAAGCCA CACAAATGCA CTTTTGAAGG TTGTTCAAAA 
GCCTACTCCA GACTAGAAAA CTTGAAAACT CACCTGAGAT CTCACACTGG AGAGAAACCA 
TATGTCTGTG AGCATGAGGG CTGCAACAAG GCCTTTTCAA ATGCATCTGA TAGAGCCAAG 
CACCAAAACC GCACTCATTC AAATGAGAAA CCTTATGTTT GCAAGATCCC AGGGTGTACA 
AAACGTTACA CTGACCCTAG CTCACTTAGA AAGCATGTAA AGACAGTGCA TGGTCCAGAG 
GCTCATGTAA CTAAGAAGCA GAGAGGGGAC ATACACCCAA GGCCTCCACC ACCACGAGAA 
CCAGGAAGCC ATTCGCAGTC AAGATCACCA GGCCAACATA TCCAGGGTAC ACATGGAGAG 
CACAAGGACC TCAGTAACAC TACCTCAAAG CATGAAGAAT GCCTGCAAGT AAAATCAGTC 
AAGACTGAAA AGCCAATGAC ATCTCAGCCA AGCCCTGGTG GTAAGTCTTC ATGCAGCAGC 
GAGCAGTCCC CCGTCAGCAA CTATACCAAC AGTGGGATCG AGCTTACTCT AAACAGTGGA 
GGCAGCATTG GAGACTTCAG TGCCCTAGAT GAAACCCCAA TCATGGACTC TACCATTTCC 
ACCGCAACCA CAGGACTTGG TTTACACGGA AGGAGGATTA TGACAGGAAC CAAATGGATG 
GAGCAAATTA AAGTTGAAAA AATTAAACAA GTTAATGGTA TTCTTCCAAG ACTGAACCCA 
GTTCCACCTC ATAGAGCACC AAATTTACCA CCAATCACTG GAAATGGTGT GCAGCTGAAC 
AGCAACATCA GTTTGGGGGG ATCAGCACCT GTTCTTCCAA ACAAAAATGA GCTTTCTAGC 
ATGGACATTA CAGTCTTGAA TATGCTTAAC AGAAGAGATA GCAATACTAG CACAATAAGT 
TCAGCATATA TGAGCAGCCG CAGGTCCTCT GGAATTTCAC CTTGTTTTTC CAGTCGAAGA 
TCCAGTGAGG CATCTCAGTT TGAGGGACGG GTTCAGAATA TAAGTGTTGC AGACTCCTAT 
GATCCAATTT CTACTGATGC CTCAAGACGA TCAAGTGAAA CAAGCCAGTG TGATGGGCTG 
CCAAGCCTCC TAAGCCTTAC TCCAGCACAG CAATACAGGC TGAAAGCTAA ATATGCTGCT 
GCTACAGGTG GGCCCCCACC AACACCACTG CCGAACATGG AAAGAATGAG TCTAAAAACC 
AGAATAGCTC TCCTGGGTGA CTCAAGAGAT TGTAGATTGT CACCTCTTCC TTCAGTCAAT 
GTCCCACGAA GATGTAGTGA TGGTGGAGTA GGCTCTTATG GAAGAAGGCA TTTGTTGTCA 
AATGAACTTC AAGGTAATGG AATGCGTAGA GCAAGTGATC CTGTAAAAAT GGTCTCTGAT 
AACTTTTCTA ATGCTAGAGT TCCAAGGTTC AACAGTCTTA ACAACATCAC CCCTCCTGAT 
GCCCCGCCAC TTATGGAAAG GCGCAATTTA AGTCTTCAGA ACTATACCCG TAATGTGTAT 
TCACCATGCC CTCCTAGTAT AAGTGAAAAT GTTGCCCTGG AAGCAATGAT AATGGAAGCA 
GAAGGCAATT TTGATGATGA GGATTTACTG CCAGATGACA TGGTTCAGTA TCTTAACTCT 
CGGAACCAAA GACTTTATGA CATTTTTGAA ACAGATAAAT TACAGAATAA TGCTGGAGAA 
AGAAATGGAA ACAACTTTGA ACCATCTCAT GTAACCAGTA ATATAATAAA TGAACAGTTC 
CTCAATTCCG AACAAACAGA TACCATAGAA AATAAAAATG ACCTCCCAAT TCAGTGGAAT 
GAAGTAAGCT CTGGCAGTGC AGAATATTCT CCATCGAGAT TAAAGTATGG GCAGCGTTTA 
ACCACACAGC AGAATCGACC TTTTGGATTG TATAATAACA TGATGGTCCA GCAGCAAAAT 
GTACCAAAGA ACGGGTTGCC CCAGCAAAGG AGCTATCAAC ATCATACACA AAACAACCCA 
CAGGTGCCCC AGCAAAATCT GAACCTTTAC AATAACAGCA GTGTGTGGAG TGGTCAGTCA 
GTCAGGGGCA ATCAGTGCAT TGACATAGCA AGATCATCAC TGGGAACGTC TGCAACAGGA 
AGTCTTTGCA ATACAACTCG AGGACAAAAA TTTACAGCCA ATGGTTTGCA AATGAATATT 
GGTCAACAAA ACTATGGACC ATCCACACAT TATAGTGTGC AATCAGCTAG CAGAACTGAC 
CCAGTCCAGA ATGAAAACAT CATGAATCAG GAATTTATGC ACCAGATGAC AGCCGAACAC 
CTAATTTATG GTATGCATTT CCTGGGGGTA ACTCGATCTA ATCAGACAAC ATCAGGGCAA 
AATGGAAACA CAATTGATGC AACACAAAGT TTCCTGTCAA CTACTCAAAA TGCTGGAGAG 
CAGCAGCCAT CCCTGGCAAA GAATATCCAG TCTTTTGTAA ACTCTAGTGG AAATAGCAGG 
CAGAATATAC ATAGAAACAA CCTGTCATAT CTGCAAGAGC AAATATGTGA CACCAACCAA 
CAAGTTTGCA GAGTGAATGG TATTAAAATG GAAATGCAGA ATCACCCACA ACAGCACTGT 
GCGAATGCAC AAAACTTCTC CGGTCAGTTG TATGACCAAA CAGCAGCATA TCCTCAGCAA 
AGTATCAAGT CAGGTTCTGA GCCTGGTATG GAAGCAAACT GTCTTTTACA AGAAAGTAAT 
AATACAAACT CATCCCAGCT TCTTTCGCCT GGGGCAAACC AAGTGACTAG CACAGTCAAT 
AACATTGACA GCAGTTTGGA TGAGGTGCAA ATCGATTTTG ATGCTATAAT TGATGATGGG 
GATCATGCCA GCCTCATTTC TGGAGCTCTA AGTCCAAGTA TCATCCAGAA TCTCTCCCGT 
AATTCTTCAC GCCTTACCAC TCCTCGAACA TCTCTGACAT TCCCAGCCAT ACCTGTTAGT 
ACCAGTAACA TGGCTATTGG GGACATGAGC TCTTTGCTGA CCTCACTTGC AGAAGAAAGC 
AAGTTTCTTG CTATTATGCA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012820946.1
CDS	1363..5886
Translation	MQPHGGQGLGKINEEPSTSSEERASLIKKELHGSITHLPEPSVPYRGTVFAMDPRNGYIDPHYHPPHLFPSFHPPVPIDARHHEGRYHYEPTPIPPLHVPSALASSPTYPDLPFIRISPHRNPASASDSPFSPPHPYISPYMDYIRSLHSSPSLSMISAARGLSPTDVPHAGVSPAEYYHQMALLTGQRSPYADIIPSPATAGAGANALHMEYLHAMESSRFSSPRVPLRPSRKRTLSISPLSDHSFDLQTMIRNSPNSLVTILNNSRSSSSASGSYGHLAASAISPALSFTYQPTPVSLQQMHQQLMSRQHSIGSAFGHSPPLLHPAPTFPSQRTIPGIPSVLNPVQVSIGPSEAAQQNKPTSESAVSSTGDPLHNKRSKVKPEEDLPSPGAVCIQDQPDGMTLVKEEGDKDESKQEPEVVYETNCHWEGCSREFDTQEQLVHHINNDHIHGEKKEFVCRWLDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCSKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDIHPRPPPPREPGSHSQSRSPGQHIQGTHGEHKDLSNTTSKHEECLQVKSVKTEKPMTSQPSPGGKSSCSSEQSPVSNYTNSGIELTLNSGGSIGDFSALDETPIMDSTISTATTGLGLHGRRIMTGTKWMEQIKVEKIKQVNGILPRLNPVPPHRAPNLPPITGNGVQLNSNISLGGSAPVLPNKNELSSMDITVLNMLNRRDSNTSTISSAYMSSRRSSGISPCFSSRRSSEASQFEGRVQNISVADSYDPISTDASRRSSETSQCDGLPSLLSLTPAQQYRLKAKYAAATGGPPPTPLPNMERMSLKTRIALLGDSRDCRLSPLPSVNVPRRCSDGGVGSYGRRHLLSNELQGNGMRRASDPVKMVSDNFSNARVPRFNSLNNITPPDAPPLMERRNLSLQNYTRNVYSPCPPSISENVALEAMIMEAEGNFDDEDLLPDDMVQYLNSRNQRLYDIFETDKLQNNAGERNGNNFEPSHVTSNIINEQFLNSEQTDTIENKNDLPIQWNEVSSGSAEYSPSRLKYGQRLTTQQNRPFGLYNNMMVQQQNVPKNGLPQQRSYQHHTQNNPQVPQQNLNLYNNSSVWSGQSVRGNQCIDIARSSLGTSATGSLCNTTRGQKFTANGLQMNIGQQNYGPSTHYSVQSASRTDPVQNENIMNQEFMHQMTAEHLIYGMHFLGVTRSNQTTSGQNGNTIDATQSFLSTTQNAGEQQPSLAKNIQSFVNSSGNSRQNIHRNNLSYLQEQICDTNQQVCRVNGIKMEMQNHPQQHCANAQNFSGQLYDQTAAYPQQSIKSGSEPGMEANCLLQESNNTNSSQLLSPGANQVTSTVNNIDSSLDEVQIDFDAIIDDGDHASLISGALSPSIIQNLSRNSSRLTTPRTSLTFPAIPVSTSNMAIGDMSSLLTSLAEESKFLAIMQ

Target ORF information:

RefSeq Version	XM_012965492.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis GLI family zinc finger 3 (gli3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965492.2

ORF Insert Sequence:

ATGCAGCCTC ATGGAGGCCA AGGTCTTGGT AAAATCAATG AGGAACCCTC TACGTCAAGT 
GAGGAAAGGG CCTCATTAAT AAAAAAAGAA CTTCATGGAT CTATAACACA TCTGCCTGAA 
CCCTCAGTTC CCTACCGTGG CACTGTCTTT GCCATGGACC CTAGGAATGG TTATATTGAT 
CCACATTACC ATCCGCCTCA TCTCTTCCCA TCTTTTCATC CTCCTGTGCC AATTGATGCA 
AGACATCATG AGGGAAGATA CCATTATGAG CCAACTCCCA TTCCGCCACT GCATGTGCCT 
TCTGCATTAG CAAGCAGCCC GACATACCCA GATCTTCCAT TTATCCGGAT ATCTCCACAC 
AGAAACCCAG CCAGTGCATC TGATTCCCCA TTCAGCCCTC CTCATCCTTA CATCAGTCCT 
TACATGGACT ATATCAGATC TCTCCACAGC AGCCCTTCAC TGTCTATGAT ATCAGCTGCC 
CGTGGACTCA GCCCTACTGA TGTCCCACAT GCTGGTGTTA GCCCTGCTGA ATATTACCAT 
CAAATGGCAC TGCTAACTGG ACAGAGAAGC CCATATGCTG ATATTATTCC ATCCCCTGCA 
ACTGCTGGTG CTGGAGCTAA TGCCCTCCAT ATGGAATATC TTCATGCCAT GGAGAGCTCA 
CGGTTTTCAA GTCCTAGAGT GCCATTACGA CCAAGTCGCA AGAGAACACT GTCCATATCA 
CCTCTTTCTG ATCATAGCTT TGATCTCCAG ACAATGATTC GCAACTCTCC AAATTCTCTT 
GTTACTATAC TTAACAACTC CCGCAGCAGC TCATCTGCTA GTGGCTCTTA TGGGCATCTA 
GCAGCCAGTG CCATCAGTCC AGCATTGAGT TTTACATACC AGCCTACACC AGTGTCTCTT 
CAGCAAATGC ATCAACAGCT TATGAGTCGA CAGCACAGCA TAGGCTCAGC TTTTGGACAC 
AGCCCTCCAC TTCTTCACCC AGCACCAACA TTTCCTTCTC AAAGGACCAT TCCCGGCATC 
CCCAGTGTTT TGAATCCAGT GCAGGTCAGC ATTGGGCCAT CAGAGGCTGC ACAGCAAAAC 
AAGCCCACCA GTGAGTCAGC AGTCAGCAGC ACAGGTGACC CCCTGCACAA CAAGCGTTCT 
AAGGTAAAAC CTGAAGAAGA TCTTCCAAGC CCTGGAGCAG TGTGTATTCA GGATCAGCCA 
GATGGAATGA CTCTGGTGAA AGAGGAGGGG GACAAAGATG AAAGTAAGCA AGAGCCTGAA 
GTGGTCTACG AGACAAACTG CCATTGGGAA GGCTGCTCGC GAGAGTTTGA CACACAGGAA 
CAGCTAGTGC ATCATATCAA CAATGACCAC ATACATGGAG AGAAAAAGGA GTTTGTGTGC 
CGATGGCTAG ATTGTTCAAG AGAACAAAAG CCGTTTAAGG CTCAGTACAT GCTGGTGGTC 
CACATGAGAA GACATACAGG AGAAAAGCCA CACAAATGCA CTTTTGAAGG TTGTTCAAAA 
GCCTACTCCA GACTAGAAAA CTTGAAAACT CACCTGAGAT CTCACACTGG AGAGAAACCA 
TATGTCTGTG AGCATGAGGG CTGCAACAAG GCCTTTTCAA ATGCATCTGA TAGAGCCAAG 
CACCAAAACC GCACTCATTC AAATGAGAAA CCTTATGTTT GCAAGATCCC AGGGTGTACA 
AAACGTTACA CTGACCCTAG CTCACTTAGA AAGCATGTAA AGACAGTGCA TGGTCCAGAG 
GCTCATGTAA CTAAGAAGCA GAGAGGGGAC ATACACCCAA GGCCTCCACC ACCACGAGAA 
CCAGGAAGCC ATTCGCAGTC AAGATCACCA GGCCAACATA TCCAGGGTAC ACATGGAGAG 
CACAAGGACC TCAGTAACAC TACCTCAAAG CATGAAGAAT GCCTGCAAGT AAAATCAGTC 
AAGACTGAAA AGCCAATGAC ATCTCAGCCA AGCCCTGGTG GTAAGTCTTC ATGCAGCAGC 
GAGCAGTCCC CCGTCAGCAA CTATACCAAC AGTGGGATCG AGCTTACTCT AAACAGTGGA 
GGCAGCATTG GAGACTTCAG TGCCCTAGAT GAAACCCCAA TCATGGACTC TACCATTTCC 
ACCGCAACCA CAGGACTTGG TTTACACGGA AGGAGGATTA TGACAGGAAC CAAATGGATG 
GAGCAAATTA AAGTTGAAAA AATTAAACAA GTTAATGGTA TTCTTCCAAG ACTGAACCCA 
GTTCCACCTC ATAGAGCACC AAATTTACCA CCAATCACTG GAAATGGTGT GCAGCTGAAC 
AGCAACATCA GTTTGGGGGG ATCAGCACCT GTTCTTCCAA ACAAAAATGA GCTTTCTAGC 
ATGGACATTA CAGTCTTGAA TATGCTTAAC AGAAGAGATA GCAATACTAG CACAATAAGT 
TCAGCATATA TGAGCAGCCG CAGGTCCTCT GGAATTTCAC CTTGTTTTTC CAGTCGAAGA 
TCCAGTGAGG CATCTCAGTT TGAGGGACGG GTTCAGAATA TAAGTGTTGC AGACTCCTAT 
GATCCAATTT CTACTGATGC CTCAAGACGA TCAAGTGAAA CAAGCCAGTG TGATGGGCTG 
CCAAGCCTCC TAAGCCTTAC TCCAGCACAG CAATACAGGC TGAAAGCTAA ATATGCTGCT 
GCTACAGGTG GGCCCCCACC AACACCACTG CCGAACATGG AAAGAATGAG TCTAAAAACC 
AGAATAGCTC TCCTGGGTGA CTCAAGAGAT TGTAGATTGT CACCTCTTCC TTCAGTCAAT 
GTCCCACGAA GATGTAGTGA TGGTGGAGTA GGCTCTTATG GAAGAAGGCA TTTGTTGTCA 
AATGAACTTC AAGGTAATGG AATGCGTAGA GCAAGTGATC CTGTAAAAAT GGTCTCTGAT 
AACTTTTCTA ATGCTAGAGT TCCAAGGTTC AACAGTCTTA ACAACATCAC CCCTCCTGAT 
GCCCCGCCAC TTATGGAAAG GCGCAATTTA AGTCTTCAGA ACTATACCCG TAATGTGTAT 
TCACCATGCC CTCCTAGTAT AAGTGAAAAT GTTGCCCTGG AAGCAATGAT AATGGAAGCA 
GAAGGCAATT TTGATGATGA GGATTTACTG CCAGATGACA TGGTTCAGTA TCTTAACTCT 
CGGAACCAAA GACTTTATGA CATTTTTGAA ACAGATAAAT TACAGAATAA TGCTGGAGAA 
AGAAATGGAA ACAACTTTGA ACCATCTCAT GTAACCAGTA ATATAATAAA TGAACAGTTC 
CTCAATTCCG AACAAACAGA TACCATAGAA AATAAAAATG ACCTCCCAAT TCAGTGGAAT 
GAAGTAAGCT CTGGCAGTGC AGAATATTCT CCATCGAGAT TAAAGTATGG GCAGCGTTTA 
ACCACACAGC AGAATCGACC TTTTGGATTG TATAATAACA TGATGGTCCA GCAGCAAAAT 
GTACCAAAGA ACGGGTTGCC CCAGCAAAGG AGCTATCAAC ATCATACACA AAACAACCCA 
CAGGTGCCCC AGCAAAATCT GAACCTTTAC AATAACAGCA GTGTGTGGAG TGGTCAGTCA 
GTCAGGGGCA ATCAGTGCAT TGACATAGCA AGATCATCAC TGGGAACGTC TGCAACAGGA 
AGTCTTTGCA ATACAACTCG AGGACAAAAA TTTACAGCCA ATGGTTTGCA AATGAATATT 
GGTCAACAAA ACTATGGACC ATCCACACAT TATAGTGTGC AATCAGCTAG CAGAACTGAC 
CCAGTCCAGA ATGAAAACAT CATGAATCAG GAATTTATGC ACCAGATGAC AGCCGAACAC 
CTAATTTATG GTATGCATTT CCTGGGGGTA ACTCGATCTA ATCAGACAAC ATCAGGGCAA 
AATGGAAACA CAATTGATGC AACACAAAGT TTCCTGTCAA CTACTCAAAA TGCTGGAGAG 
CAGCAGCCAT CCCTGGCAAA GAATATCCAG TCTTTTGTAA ACTCTAGTGG AAATAGCAGG 
CAGAATATAC ATAGAAACAA CCTGTCATAT CTGCAAGAGC AAATATGTGA CACCAACCAA 
CAAGTTTGCA GAGTGAATGG TATTAAAATG GAAATGCAGA ATCACCCACA ACAGCACTGT 
GCGAATGCAC AAAACTTCTC CGGTCAGTTG TATGACCAAA CAGCAGCATA TCCTCAGCAA 
AGTATCAAGT CAGGTTCTGA GCCTGGTATG GAAGCAAACT GTCTTTTACA AGAAAGTAAT 
AATACAAACT CATCCCAGCT TCTTTCGCCT GGGGCAAACC AAGTGACTAG CACAGTCAAT 
AACATTGACA GCAGTTTGGA TGAGGTGCAA ATCGATTTTG ATGCTATAAT TGATGATGGG 
GATCATGCCA GCCTCATTTC TGGAGCTCTA AGTCCAAGTA TCATCCAGAA TCTCTCCCGT 
AATTCTTCAC GCCTTACCAC TCCTCGAACA TCTCTGACAT TCCCAGCCAT ACCTGTTAGT 
ACCAGTAACA TGGCTATTGG GGACATGAGC TCTTTGCTGA CCTCACTTGC AGAAGAAAGC 
AAGTTTCTTG CTATTATGCA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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gli3 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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