ube4a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ube4a
Entrez Gene ID	100101757
Full Name	ubiquitination factor E4A
Synonyms	ubox2,ufd2
General protein information	Preferred Names ubiquitination factor E4A Names ubiquitin conjugation factor E4 A ubiquitination factor E4A ubiquitination factor E4A (UFD2 homolog)
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012967018.1	XP_012822472.1	ubiquitin conjugation factor E4 A isoform X1
XM_012967018.2	XP_012822472.1	ubiquitin conjugation factor E4 A isoform X1
XM_012967019.2	XP_012822473.1	ubiquitin conjugation factor E4 A isoform X3
XM_012967019.3	XP_012822473.1	ubiquitin conjugation factor E4 A isoform X2
XM_018096066.1	XP_017951555.1	ubiquitin conjugation factor E4 A isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	UBE4A	NP_004779.2
Bos taurus (cattle)	UBE4A	NP_001092469.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001681	XP_321414.5
Pan troglodytes (chimpanzee)	UBE4A	XP_508791.2
Xenopus tropicalis (tropical clawed frog)	ube4a	NP_001163971.1
Canis lupus familiaris (dog)	UBE4A	XP_850081.1
Mus musculus (house mouse)	Ube4a	NP_663375.3
Danio rerio (zebrafish)	ube4a	XP_001922961.2
Drosophila melanogaster (fruit fly)	CG11070	NP_609060.1
	UBE4A	XP_001097911.2
Rattus norvegicus (Norway rat)	Ube4a	NP_997493.1
Gallus gallus (chicken)	UBE4A	NP_001026081.1

Gene Ontology

The following ube4a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ube4a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa30244	XM_012967019.3 Latest version!	Xenopus tropicalis ubiquitination factor E4A (ube4a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa30243	XM_012967018.2 Latest version!	Xenopus tropicalis ubiquitination factor E4A (ube4a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa45914	XM_018096066.1 Latest version!	Xenopus tropicalis ubiquitination factor E4A (ube4a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
OXa30243	XM_012967018.1	Xenopus tropicalis ubiquitination factor E4A (ube4a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa30244	XM_012967019.2	Xenopus tropicalis ubiquitination factor E4A (ube4a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ube4a ( XM_012967019.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822473.1 Xenopus tropicalis ubiquitination factor E4A (ube4a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa30244
Clone ID Related Accession (Same CDS sequence)	XM_012967019.2 , XM_012967019.3
Accession Version	XM_012967019.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ubiquitin conjugation factor E4 A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012967019.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGACC AAGAAAACAA CAACAGCTTT TCAGCCAATC CTTTTGCTGC CCTGTTTGGG 
TCTTTGGCTG AGGCTCGCCA ATTTGTGGCT GGTCAGAAAG AGCAACTCAG GCAGACTGAG 
GATGATGCTG AAGAGAGCCA AGATGAGTCA GACAACAGCG TGTCAGAAAG CCTGGATGAT 
TGTGATGACT CTGTGGCAGA AATCAGTCGA TCTTTTCGGT CCCAGCAGGA AATCTGTGAG 
CAGCTAAACA TTAATCACAT GATCCAAAGG ATCTTCCTCA TTACCTTGGA CAACAGTGAT 
CCCAGTCTGA AGAGTGGGAA TGGAATTCCT TTACGTTGTG TGTATTTGGA AGAGCTTGGA 
TCAGAGCTGG ATGGGCAGGA CTGGCTTGAC ATGGATAATG TTGAACAGGC TCTGTTTACA 
CGACTACTTC TCCCAGAGCC AGGAAAGTAT CTAATCTGTA TGACATCCTC TGGAGCCCCC 
AATTTATCTG CAGAGAGAGA TGCTGGAGAG AAACAAATAA TCCCATTCTT ATTTTCCTGT 
TTTCGTAGAG CCAAGAAAGA GGTCACAAAA GTGCCTGAGA ACCTTTTACC ATATGCTGTA 
CGCTGTAAAG CTCTGACTGT ATCCAATGCT CGTACTGTTC TACTGACCCC AGAGATATAC 
CATGGGCAGG ATATTGCCAC ACAGTTGGTG GACTTGATGC TAGAAGAACT CAGAGGAGCA 
AATTTTGAAG AGGTAAAGGA GTTCTTGGAG GAGGTGATAG AGGCTCTTCT GAAAGATGAG 
GAGGTCCGCT CTTTTAGTGA GGTCTTCCAC CCAGTATTCG ATGTTCTCCT GGAGCGCGTA 
CAGGATTTGG ACCTTTGTCA GATGCTACTC TACACTTACC TTGACCTTCT TCTATACTGC 
ACCCACCAGA AGGACATTGC CAAGGTGTTT GTGGAATATA TTCAGCCACA GGACCCCACA 
AACGGGCATC AGTATCAGAA GACTCTGTTG GGTGTAATCC TTAATATCTC TTGTTTACTG 
CGCACGCCAG GGGTAGTTGA GAATCATGGC TTCTTTATAA ATCCATCTCG CTCCAGCCCT 
CAAGAGATCA AAGTACAGGA ATCTAATATA CATCAGTTTA TGGCTCAGTT TCATGAGAAG 
ATTTATCAAC TTCTGAAGAA CCTACTTCAG TTATCCCCAG ATACTAAGCA CCGTATTTTG 
TCCTGGCTGG GGAACTGCCT GCATGCCAAT GCTGGTCGTA CAAAAATCTG GGCTAACCAG 
GTGCCCGAGA TCTTCATGCA AACCTATGCA TCTGACAGTT TCTTCCTGAA CCTTGGAGCA 
GCCTTACTCC GCCTCTGCCA ACCATTCTCT AAGCCACGTT CTGCACGTCT GCTGACTTTT 
AACCCCACCT ATTGTGCTCT AAAAGAAGTC AATGAGGAGG AACGCCGGAG CAGGAATATG 
CACATGAAAG GGTTGGATAA GGAGACCTGC TTGATACCAG CTGCTGCTGA CCAGCAGCCT 
GACTTTCCTG AGAACTTCAA CTTGGTGACA GAAAACCTAG TGTTGACACA ATACACATTG 
CACTTAGGCT TCCATAGGTT GCATGAGCAA ATGGTTAAGG TCAACCAGAG CCTGCATCGG 
CTTCAGGGTG CATGGAGAGA TGCCCAGCAG AGTGGAAGCC CCACAGCAGA GAACCTTAGA 
GAACAGTTTG AGCGGCTTAT GACCATTTAC CTATGTCTGA AAACAGCTTT GAGTGAACCC 
CAAATGCTTC AGAACTGTAT TCATCTTCAA GTGTCAACTG CACTGCTCTT AGTGCAAATT 
GCATATGGCA ACAAAGGCAC AGAACCAGTG GCCCTCTCTT TTCCTATGCC TAATGTACAG 
CACAGTGCTC TGGCTTATGT ACCAGAATTC TTTGCTGATA ATTTGGGGGA TTTCTTCATA 
TTCCTACGCC GTTTTGCAGA TGAAGTTTTG GAAACTGCAG CAGACTCCTT AGAGCAAATT 
CTTGATTTTA TCACTGTTTT TACTGGCAGT GTTGAGAGAA TGAAGAACCC CCACTTGCGG 
GCCAAGCTGG CAGAGGTCCT AGAGGCAGTG ATGCCACATC TGGAACAGGT GCAAAACCCC 
CTGATTTCTA GTGTGTTCCA TCGGCAGCGT ATCTTCTGTT CATACCAACA TGCCCCTCAC 
TTGGCGGAAG CCCTCATTAA GGTGTTTGTT GACATTGAAT TCACAGGGGA CCCTCATCAG 
TTTGAACAGA AGTTTAATTA TCGGCGACCA ATGTATCCCA TACTAAAGTA CATGTGGGGA 
AGAGACAATT ACAGACAAAG TATAAAGAAC CTAGCTGACT ATGCCTCTAA AAATCTGGAA 
GCAGTGAACC CACCTCTCTT TTTACGGTTC CTTAATCTCC TCATGAATGA TGCTGTATTT 
CTGCTTGATG AGGCAATCCA GTATCTGAGC AAGATTAAAG TTCTGCAGAT AGAAAGGGAC 
CGAGGTGAAT GGGATGCATT GAGCCCTGAG AGCCGGCGGG AAAAGGAGTC CAACCTCCTT 
ATGTTTGGCC AGCTGGCCCG ATTTCATAAT ATCATGTCGA ATGAGACCAT TGGAACCTTG 
GCCTTCCTTA CCTCAGATAT ACGTTCTCTC TTTGTTCAGC CATTTCTTGC TGATCGCACT 
ATTTCCATGC TCAACTACTT CCTGCAGCAT CTTGTCGGGC CTAAGATGGG GGCCCTGAAG 
GTGAAAGATT TCAGTGAGTT TGACTTCAAG CCACAGCAAT TGGTGTCTGA CATCTGCACA 
ATCTACCTAA ATCTCGGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822473.1
CDS	1014..3794
Translation	MTDQENNNSFSANPFAALFGSLAEARQFVAGQKEQLRQTEDDAEESQDESDNSVSESLDDCDDSVAEISRSFRSQQEICEQLNINHMIQRIFLITLDNSDPSLKSGNGIPLRCVYLEELGSELDGQDWLDMDNVEQALFTRLLLPEPGKYLICMTSSGAPNLSAERDAGEKQIIPFLFSCFRRAKKEVTKVPENLLPYAVRCKALTVSNARTVLLTPEIYHGQDIATQLVDLMLEELRGANFEEVKEFLEEVIEALLKDEEVRSFSEVFHPVFDVLLERVQDLDLCQMLLYTYLDLLLYCTHQKDIAKVFVEYIQPQDPTNGHQYQKTLLGVILNISCLLRTPGVVENHGFFINPSRSSPQEIKVQESNIHQFMAQFHEKIYQLLKNLLQLSPDTKHRILSWLGNCLHANAGRTKIWANQVPEIFMQTYASDSFFLNLGAALLRLCQPFSKPRSARLLTFNPTYCALKEVNEEERRSRNMHMKGLDKETCLIPAAADQQPDFPENFNLVTENLVLTQYTLHLGFHRLHEQMVKVNQSLHRLQGAWRDAQQSGSPTAENLREQFERLMTIYLCLKTALSEPQMLQNCIHLQVSTALLLVQIAYGNKGTEPVALSFPMPNVQHSALAYVPEFFADNLGDFFIFLRRFADEVLETAADSLEQILDFITVFTGSVERMKNPHLRAKLAEVLEAVMPHLEQVQNPLISSVFHRQRIFCSYQHAPHLAEALIKVFVDIEFTGDPHQFEQKFNYRRPMYPILKYMWGRDNYRQSIKNLADYASKNLEAVNPPLFLRFLNLLMNDAVFLLDEAIQYLSKIKVLQIERDRGEWDALSPESRREKESNLLMFGQLARFHNIMSNETIGTLAFLTSDIRSLFVQPFLADRTISMLNYFLQHLVGPKMGALKVKDFSEFDFKPQQLVSDICTIYLNLG

Target ORF information:

RefSeq Version	XM_012967019.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitination factor E4A (ube4a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967019.2

ORF Insert Sequence:

ATGACGGACC AAGAAAACAA CAACAGCTTT TCAGCCAATC CTTTTGCTGC CCTGTTTGGG 
TCTTTGGCTG AGGCTCGCCA ATTTGTGGCT GGTCAGAAAG AGCAACTCAG GCAGACTGAG 
GATGATGCTG AAGAGAGCCA AGATGAGTCA GACAACAGCG TGTCAGAAAG CCTGGATGAT 
TGTGATGACT CTGTGGCAGA AATCAGTCGA TCTTTTCGGT CCCAGCAGGA AATCTGTGAG 
CAGCTAAACA TTAATCACAT GATCCAAAGG ATCTTCCTCA TTACCTTGGA CAACAGTGAT 
CCCAGTCTGA AGAGTGGGAA TGGAATTCCT TTACGTTGTG TGTATTTGGA AGAGCTTGGA 
TCAGAGCTGG ATGGGCAGGA CTGGCTTGAC ATGGATAATG TTGAACAGGC TCTGTTTACA 
CGACTACTTC TCCCAGAGCC AGGAAAGTAT CTAATCTGTA TGACATCCTC TGGAGCCCCC 
AATTTATCTG CAGAGAGAGA TGCTGGAGAG AAACAAATAA TCCCATTCTT ATTTTCCTGT 
TTTCGTAGAG CCAAGAAAGA GGTCACAAAA GTGCCTGAGA ACCTTTTACC ATATGCTGTA 
CGCTGTAAAG CTCTGACTGT ATCCAATGCT CGTACTGTTC TACTGACCCC AGAGATATAC 
CATGGGCAGG ATATTGCCAC ACAGTTGGTG GACTTGATGC TAGAAGAACT CAGAGGAGCA 
AATTTTGAAG AGGTAAAGGA GTTCTTGGAG GAGGTGATAG AGGCTCTTCT GAAAGATGAG 
GAGGTCCGCT CTTTTAGTGA GGTCTTCCAC CCAGTATTCG ATGTTCTCCT GGAGCGCGTA 
CAGGATTTGG ACCTTTGTCA GATGCTACTC TACACTTACC TTGACCTTCT TCTATACTGC 
ACCCACCAGA AGGACATTGC CAAGGTGTTT GTGGAATATA TTCAGCCACA GGACCCCACA 
AACGGGCATC AGTATCAGAA GACTCTGTTG GGTGTAATCC TTAATATCTC TTGTTTACTG 
CGCACGCCAG GGGTAGTTGA GAATCATGGC TTCTTTATAA ATCCATCTCG CTCCAGCCCT 
CAAGAGATCA AAGTACAGGA ATCTAATATA CATCAGTTTA TGGCTCAGTT TCATGAGAAG 
ATTTATCAAC TTCTGAAGAA CCTACTTCAG TTATCCCCAG ATACTAAGCA CCGTATTTTG 
TCCTGGCTGG GGAACTGCCT GCATGCCAAT GCTGGTCGTA CAAAAATCTG GGCTAACCAG 
GTGCCCGAGA TCTTCATGCA AACCTATGCA TCTGACAGTT TCTTCCTGAA CCTTGGAGCA 
GCCTTACTCC GCCTCTGCCA ACCATTCTCT AAGCCACGTT CTGCACGTCT GCTGACTTTT 
AACCCCACCT ATTGTGCTCT AAAAGAAGTC AATGAGGAGG AACGCCGGAG CAGGAATATG 
CACATGAAAG GGTTGGATAA GGAGACCTGC TTGATACCAG CTGCTGCTGA CCAGCAGCCT 
GACTTTCCTG AGAACTTCAA CTTGGTGACA GAAAACCTAG TGTTGACACA ATACACATTG 
CACTTAGGCT TCCATAGGTT GCATGAGCAA ATGGTTAAGG TCAACCAGAG CCTGCATCGG 
CTTCAGGGTG CATGGAGAGA TGCCCAGCAG AGTGGAAGCC CCACAGCAGA GAACCTTAGA 
GAACAGTTTG AGCGGCTTAT GACCATTTAC CTATGTCTGA AAACAGCTTT GAGTGAACCC 
CAAATGCTTC AGAACTGTAT TCATCTTCAA GTGTCAACTG CACTGCTCTT AGTGCAAATT 
GCATATGGCA ACAAAGGCAC AGAACCAGTG GCCCTCTCTT TTCCTATGCC TAATGTACAG 
CACAGTGCTC TGGCTTATGT ACCAGAATTC TTTGCTGATA ATTTGGGGGA TTTCTTCATA 
TTCCTACGCC GTTTTGCAGA TGAAGTTTTG GAAACTGCAG CAGACTCCTT AGAGCAAATT 
CTTGATTTTA TCACTGTTTT TACTGGCAGT GTTGAGAGAA TGAAGAACCC CCACTTGCGG 
GCCAAGCTGG CAGAGGTCCT AGAGGCAGTG ATGCCACATC TGGAACAGGT GCAAAACCCC 
CTGATTTCTA GTGTGTTCCA TCGGCAGCGT ATCTTCTGTT CATACCAACA TGCCCCTCAC 
TTGGCGGAAG CCCTCATTAA GGTGTTTGTT GACATTGAAT TCACAGGGGA CCCTCATCAG 
TTTGAACAGA AGTTTAATTA TCGGCGACCA ATGTATCCCA TACTAAAGTA CATGTGGGGA 
AGAGACAATT ACAGACAAAG TATAAAGAAC CTAGCTGACT ATGCCTCTAA AAATCTGGAA 
GCAGTGAACC CACCTCTCTT TTTACGGTTC CTTAATCTCC TCATGAATGA TGCTGTATTT 
CTGCTTGATG AGGCAATCCA GTATCTGAGC AAGATTAAAG TTCTGCAGAT AGAAAGGGAC 
CGAGGTGAAT GGGATGCATT GAGCCCTGAG AGCCGGCGGG AAAAGGAGTC CAACCTCCTT 
ATGTTTGGCC AGCTGGCCCG ATTTCATAAT ATCATGTCGA ATGAGACCAT TGGAACCTTG 
GCCTTCCTTA CCTCAGATAT ACGTTCTCTC TTTGTTCAGC CATTTCTTGC TGATCGCACT 
ATTTCCATGC TCAACTACTT CCTGCAGCAT CTTGTCGGGC CTAAGATGGG GGCCCTGAAG 
GTGAAAGATT TCAGTGAGTT TGACTTCAAG CCACAGCAAT TGGTGTCTGA CATCTGCACA 
ATCTACCTAA ATCTCGGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ube4a ( XM_012967019.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822473.1 Xenopus tropicalis ubiquitination factor E4A (ube4a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa30244
Clone ID Related Accession (Same CDS sequence)	XM_012967019.2 , XM_012967019.3
Accession Version	XM_012967019.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ubiquitin conjugation factor E4 A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012967019.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGACC AAGAAAACAA CAACAGCTTT TCAGCCAATC CTTTTGCTGC CCTGTTTGGG 
TCTTTGGCTG AGGCTCGCCA ATTTGTGGCT GGTCAGAAAG AGCAACTCAG GCAGACTGAG 
GATGATGCTG AAGAGAGCCA AGATGAGTCA GACAACAGCG TGTCAGAAAG CCTGGATGAT 
TGTGATGACT CTGTGGCAGA AATCAGTCGA TCTTTTCGGT CCCAGCAGGA AATCTGTGAG 
CAGCTAAACA TTAATCACAT GATCCAAAGG ATCTTCCTCA TTACCTTGGA CAACAGTGAT 
CCCAGTCTGA AGAGTGGGAA TGGAATTCCT TTACGTTGTG TGTATTTGGA AGAGCTTGGA 
TCAGAGCTGG ATGGGCAGGA CTGGCTTGAC ATGGATAATG TTGAACAGGC TCTGTTTACA 
CGACTACTTC TCCCAGAGCC AGGAAAGTAT CTAATCTGTA TGACATCCTC TGGAGCCCCC 
AATTTATCTG CAGAGAGAGA TGCTGGAGAG AAACAAATAA TCCCATTCTT ATTTTCCTGT 
TTTCGTAGAG CCAAGAAAGA GGTCACAAAA GTGCCTGAGA ACCTTTTACC ATATGCTGTA 
CGCTGTAAAG CTCTGACTGT ATCCAATGCT CGTACTGTTC TACTGACCCC AGAGATATAC 
CATGGGCAGG ATATTGCCAC ACAGTTGGTG GACTTGATGC TAGAAGAACT CAGAGGAGCA 
AATTTTGAAG AGGTAAAGGA GTTCTTGGAG GAGGTGATAG AGGCTCTTCT GAAAGATGAG 
GAGGTCCGCT CTTTTAGTGA GGTCTTCCAC CCAGTATTCG ATGTTCTCCT GGAGCGCGTA 
CAGGATTTGG ACCTTTGTCA GATGCTACTC TACACTTACC TTGACCTTCT TCTATACTGC 
ACCCACCAGA AGGACATTGC CAAGGTGTTT GTGGAATATA TTCAGCCACA GGACCCCACA 
AACGGGCATC AGTATCAGAA GACTCTGTTG GGTGTAATCC TTAATATCTC TTGTTTACTG 
CGCACGCCAG GGGTAGTTGA GAATCATGGC TTCTTTATAA ATCCATCTCG CTCCAGCCCT 
CAAGAGATCA AAGTACAGGA ATCTAATATA CATCAGTTTA TGGCTCAGTT TCATGAGAAG 
ATTTATCAAC TTCTGAAGAA CCTACTTCAG TTATCCCCAG ATACTAAGCA CCGTATTTTG 
TCCTGGCTGG GGAACTGCCT GCATGCCAAT GCTGGTCGTA CAAAAATCTG GGCTAACCAG 
GTGCCCGAGA TCTTCATGCA AACCTATGCA TCTGACAGTT TCTTCCTGAA CCTTGGAGCA 
GCCTTACTCC GCCTCTGCCA ACCATTCTCT AAGCCACGTT CTGCACGTCT GCTGACTTTT 
AACCCCACCT ATTGTGCTCT AAAAGAAGTC AATGAGGAGG AACGCCGGAG CAGGAATATG 
CACATGAAAG GGTTGGATAA GGAGACCTGC TTGATACCAG CTGCTGCTGA CCAGCAGCCT 
GACTTTCCTG AGAACTTCAA CTTGGTGACA GAAAACCTAG TGTTGACACA ATACACATTG 
CACTTAGGCT TCCATAGGTT GCATGAGCAA ATGGTTAAGG TCAACCAGAG CCTGCATCGG 
CTTCAGGGTG CATGGAGAGA TGCCCAGCAG AGTGGAAGCC CCACAGCAGA GAACCTTAGA 
GAACAGTTTG AGCGGCTTAT GACCATTTAC CTATGTCTGA AAACAGCTTT GAGTGAACCC 
CAAATGCTTC AGAACTGTAT TCATCTTCAA GTGTCAACTG CACTGCTCTT AGTGCAAATT 
GCATATGGCA ACAAAGGCAC AGAACCAGTG GCCCTCTCTT TTCCTATGCC TAATGTACAG 
CACAGTGCTC TGGCTTATGT ACCAGAATTC TTTGCTGATA ATTTGGGGGA TTTCTTCATA 
TTCCTACGCC GTTTTGCAGA TGAAGTTTTG GAAACTGCAG CAGACTCCTT AGAGCAAATT 
CTTGATTTTA TCACTGTTTT TACTGGCAGT GTTGAGAGAA TGAAGAACCC CCACTTGCGG 
GCCAAGCTGG CAGAGGTCCT AGAGGCAGTG ATGCCACATC TGGAACAGGT GCAAAACCCC 
CTGATTTCTA GTGTGTTCCA TCGGCAGCGT ATCTTCTGTT CATACCAACA TGCCCCTCAC 
TTGGCGGAAG CCCTCATTAA GGTGTTTGTT GACATTGAAT TCACAGGGGA CCCTCATCAG 
TTTGAACAGA AGTTTAATTA TCGGCGACCA ATGTATCCCA TACTAAAGTA CATGTGGGGA 
AGAGACAATT ACAGACAAAG TATAAAGAAC CTAGCTGACT ATGCCTCTAA AAATCTGGAA 
GCAGTGAACC CACCTCTCTT TTTACGGTTC CTTAATCTCC TCATGAATGA TGCTGTATTT 
CTGCTTGATG AGGCAATCCA GTATCTGAGC AAGATTAAAG TTCTGCAGAT AGAAAGGGAC 
CGAGGTGAAT GGGATGCATT GAGCCCTGAG AGCCGGCGGG AAAAGGAGTC CAACCTCCTT 
ATGTTTGGCC AGCTGGCCCG ATTTCATAAT ATCATGTCGA ATGAGACCAT TGGAACCTTG 
GCCTTCCTTA CCTCAGATAT ACGTTCTCTC TTTGTTCAGC CATTTCTTGC TGATCGCACT 
ATTTCCATGC TCAACTACTT CCTGCAGCAT CTTGTCGGGC CTAAGATGGG GGCCCTGAAG 
GTGAAAGATT TCAGTGAGTT TGACTTCAAG CCACAGCAAT TGGTGTCTGA CATCTGCACA 
ATCTACCTAA ATCTCGGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822473.1
CDS	82..2862
Translation	MTDQENNNSFSANPFAALFGSLAEARQFVAGQKEQLRQTEDDAEESQDESDNSVSESLDDCDDSVAEISRSFRSQQEICEQLNINHMIQRIFLITLDNSDPSLKSGNGIPLRCVYLEELGSELDGQDWLDMDNVEQALFTRLLLPEPGKYLICMTSSGAPNLSAERDAGEKQIIPFLFSCFRRAKKEVTKVPENLLPYAVRCKALTVSNARTVLLTPEIYHGQDIATQLVDLMLEELRGANFEEVKEFLEEVIEALLKDEEVRSFSEVFHPVFDVLLERVQDLDLCQMLLYTYLDLLLYCTHQKDIAKVFVEYIQPQDPTNGHQYQKTLLGVILNISCLLRTPGVVENHGFFINPSRSSPQEIKVQESNIHQFMAQFHEKIYQLLKNLLQLSPDTKHRILSWLGNCLHANAGRTKIWANQVPEIFMQTYASDSFFLNLGAALLRLCQPFSKPRSARLLTFNPTYCALKEVNEEERRSRNMHMKGLDKETCLIPAAADQQPDFPENFNLVTENLVLTQYTLHLGFHRLHEQMVKVNQSLHRLQGAWRDAQQSGSPTAENLREQFERLMTIYLCLKTALSEPQMLQNCIHLQVSTALLLVQIAYGNKGTEPVALSFPMPNVQHSALAYVPEFFADNLGDFFIFLRRFADEVLETAADSLEQILDFITVFTGSVERMKNPHLRAKLAEVLEAVMPHLEQVQNPLISSVFHRQRIFCSYQHAPHLAEALIKVFVDIEFTGDPHQFEQKFNYRRPMYPILKYMWGRDNYRQSIKNLADYASKNLEAVNPPLFLRFLNLLMNDAVFLLDEAIQYLSKIKVLQIERDRGEWDALSPESRREKESNLLMFGQLARFHNIMSNETIGTLAFLTSDIRSLFVQPFLADRTISMLNYFLQHLVGPKMGALKVKDFSEFDFKPQQLVSDICTIYLNLG

Target ORF information:

RefSeq Version	XM_012967019.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitination factor E4A (ube4a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967019.3

ORF Insert Sequence:

ATGACGGACC AAGAAAACAA CAACAGCTTT TCAGCCAATC CTTTTGCTGC CCTGTTTGGG 
TCTTTGGCTG AGGCTCGCCA ATTTGTGGCT GGTCAGAAAG AGCAACTCAG GCAGACTGAG 
GATGATGCTG AAGAGAGCCA AGATGAGTCA GACAACAGCG TGTCAGAAAG CCTGGATGAT 
TGTGATGACT CTGTGGCAGA AATCAGTCGA TCTTTTCGGT CCCAGCAGGA AATCTGTGAG 
CAGCTAAACA TTAATCACAT GATCCAAAGG ATCTTCCTCA TTACCTTGGA CAACAGTGAT 
CCCAGTCTGA AGAGTGGGAA TGGAATTCCT TTACGTTGTG TGTATTTGGA AGAGCTTGGA 
TCAGAGCTGG ATGGGCAGGA CTGGCTTGAC ATGGATAATG TTGAACAGGC TCTGTTTACA 
CGACTACTTC TCCCAGAGCC AGGAAAGTAT CTAATCTGTA TGACATCCTC TGGAGCCCCC 
AATTTATCTG CAGAGAGAGA TGCTGGAGAG AAACAAATAA TCCCATTCTT ATTTTCCTGT 
TTTCGTAGAG CCAAGAAAGA GGTCACAAAA GTGCCTGAGA ACCTTTTACC ATATGCTGTA 
CGCTGTAAAG CTCTGACTGT ATCCAATGCT CGTACTGTTC TACTGACCCC AGAGATATAC 
CATGGGCAGG ATATTGCCAC ACAGTTGGTG GACTTGATGC TAGAAGAACT CAGAGGAGCA 
AATTTTGAAG AGGTAAAGGA GTTCTTGGAG GAGGTGATAG AGGCTCTTCT GAAAGATGAG 
GAGGTCCGCT CTTTTAGTGA GGTCTTCCAC CCAGTATTCG ATGTTCTCCT GGAGCGCGTA 
CAGGATTTGG ACCTTTGTCA GATGCTACTC TACACTTACC TTGACCTTCT TCTATACTGC 
ACCCACCAGA AGGACATTGC CAAGGTGTTT GTGGAATATA TTCAGCCACA GGACCCCACA 
AACGGGCATC AGTATCAGAA GACTCTGTTG GGTGTAATCC TTAATATCTC TTGTTTACTG 
CGCACGCCAG GGGTAGTTGA GAATCATGGC TTCTTTATAA ATCCATCTCG CTCCAGCCCT 
CAAGAGATCA AAGTACAGGA ATCTAATATA CATCAGTTTA TGGCTCAGTT TCATGAGAAG 
ATTTATCAAC TTCTGAAGAA CCTACTTCAG TTATCCCCAG ATACTAAGCA CCGTATTTTG 
TCCTGGCTGG GGAACTGCCT GCATGCCAAT GCTGGTCGTA CAAAAATCTG GGCTAACCAG 
GTGCCCGAGA TCTTCATGCA AACCTATGCA TCTGACAGTT TCTTCCTGAA CCTTGGAGCA 
GCCTTACTCC GCCTCTGCCA ACCATTCTCT AAGCCACGTT CTGCACGTCT GCTGACTTTT 
AACCCCACCT ATTGTGCTCT AAAAGAAGTC AATGAGGAGG AACGCCGGAG CAGGAATATG 
CACATGAAAG GGTTGGATAA GGAGACCTGC TTGATACCAG CTGCTGCTGA CCAGCAGCCT 
GACTTTCCTG AGAACTTCAA CTTGGTGACA GAAAACCTAG TGTTGACACA ATACACATTG 
CACTTAGGCT TCCATAGGTT GCATGAGCAA ATGGTTAAGG TCAACCAGAG CCTGCATCGG 
CTTCAGGGTG CATGGAGAGA TGCCCAGCAG AGTGGAAGCC CCACAGCAGA GAACCTTAGA 
GAACAGTTTG AGCGGCTTAT GACCATTTAC CTATGTCTGA AAACAGCTTT GAGTGAACCC 
CAAATGCTTC AGAACTGTAT TCATCTTCAA GTGTCAACTG CACTGCTCTT AGTGCAAATT 
GCATATGGCA ACAAAGGCAC AGAACCAGTG GCCCTCTCTT TTCCTATGCC TAATGTACAG 
CACAGTGCTC TGGCTTATGT ACCAGAATTC TTTGCTGATA ATTTGGGGGA TTTCTTCATA 
TTCCTACGCC GTTTTGCAGA TGAAGTTTTG GAAACTGCAG CAGACTCCTT AGAGCAAATT 
CTTGATTTTA TCACTGTTTT TACTGGCAGT GTTGAGAGAA TGAAGAACCC CCACTTGCGG 
GCCAAGCTGG CAGAGGTCCT AGAGGCAGTG ATGCCACATC TGGAACAGGT GCAAAACCCC 
CTGATTTCTA GTGTGTTCCA TCGGCAGCGT ATCTTCTGTT CATACCAACA TGCCCCTCAC 
TTGGCGGAAG CCCTCATTAA GGTGTTTGTT GACATTGAAT TCACAGGGGA CCCTCATCAG 
TTTGAACAGA AGTTTAATTA TCGGCGACCA ATGTATCCCA TACTAAAGTA CATGTGGGGA 
AGAGACAATT ACAGACAAAG TATAAAGAAC CTAGCTGACT ATGCCTCTAA AAATCTGGAA 
GCAGTGAACC CACCTCTCTT TTTACGGTTC CTTAATCTCC TCATGAATGA TGCTGTATTT 
CTGCTTGATG AGGCAATCCA GTATCTGAGC AAGATTAAAG TTCTGCAGAT AGAAAGGGAC 
CGAGGTGAAT GGGATGCATT GAGCCCTGAG AGCCGGCGGG AAAAGGAGTC CAACCTCCTT 
ATGTTTGGCC AGCTGGCCCG ATTTCATAAT ATCATGTCGA ATGAGACCAT TGGAACCTTG 
GCCTTCCTTA CCTCAGATAT ACGTTCTCTC TTTGTTCAGC CATTTCTTGC TGATCGCACT 
ATTTCCATGC TCAACTACTT CCTGCAGCAT CTTGTCGGGC CTAAGATGGG GGCCCTGAAG 
GTGAAAGATT TCAGTGAGTT TGACTTCAAG CCACAGCAAT TGGTGTCTGA CATCTGCACA 
ATCTACCTAA ATCTCGGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ube4a ( XM_012967018.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822472.1 Xenopus tropicalis ubiquitination factor E4A (ube4a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa30243
Clone ID Related Accession (Same CDS sequence)	XM_012967018.1 , XM_012967018.2
Accession Version	XM_012967018.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ubiquitin conjugation factor E4 A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012967018.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGACC AAGAAAACAA CAACAGCTTT TCAGCCAATC CTTTTGCTGC CCTGTTTGGG 
TCTTTGGCTG AGGCTCGCCA ATTTGTGGCT GGTCAGAAAG AGCAACTCAG GCAGACTGAG 
GATGATGCTG AAGAGAGCCA AGATGAGTCA GACAACAGCG TGTCAGAAAG CCTGGATGAT 
TGTGATGACT CTGTGGCAGA AATCAGTCGA TCTTTTCGGT CCCAGCAGGA AATCTGTGAG 
CAGCTAAACA TTAATCACAT GATCCAAAGG ATCTTCCTCA TTACCTTGGA CAACAGTGAT 
CCCAGTCTGA AGAGTGGGAA TGGAATTCCT TTACGTTGTG TGTATTTGGA AGAGCTTGGA 
TCAGAGCTGG ATGGGCAGGA CTGGCTTGAC ATGGATAATG TTGAACAGGC TCTGTTTACA 
CGACTACTTC TCCCAGAGCC AGGAAAGTAT CTAATCTGTA TGACATCCTC TGGAGCCCCC 
AATTTATCTG CAGAGAGAGA TGCTGGAGAG AAACAAATAA TCCCATTCTT ATTTTCCTGT 
TTTCGTAGAG CCAAGAAAGA GGTCACAAAA GTGCCTGAGA ACCTTTTACC ATATGCTGTA 
CGCTGTAAAG CTCTGACTGT ATCCAATGCT CGTACTGTTC TACTGACCCC AGAGATATAC 
CATGGGCAGG ATATTGCCAC ACAGTTGGTG GACTTGATGC TAGAAGAACT CAGAGGAGCA 
AATTTTGAAG AGGTAAAGGA GTTCTTGGAG GAGGTGATAG AGGCTCTTCT GAAAGATGAG 
GAGGTCCGCT CTTTTAGTGA GGTCTTCCAC CCAGTATTCG ATGTTCTCCT GGAGCGCGTA 
CAGGATTTGG ACCTTTGTCA GATGCTACTC TACACTTACC TTGACCTTCT TCTATACTGC 
ACCCACCAGA AGGACATTGC CAAGGTGTTT GTGGAATATA TTCAGCCACA GGACCCCACA 
AACGGGCATC AGTATCAGAA GACTCTGTTG GGTGTAATCC TTAATATCTC TTGTTTACTG 
CGCACGCCAG GGGTAGTTGA GAATCATGGC TTCTTTATAA ATCCATCTCG CTCCAGCCCT 
CAAGAGATCA AAGTACAGGA ATCTAATATA CATCAGTTTA TGGCTCAGTT TCATGAGAAG 
ATTTATCAAC TTCTGAAGAA CCTACTTCAG TTATCCCCAG ATACTAAGCA CCGTATTTTG 
TCCTGGCTGG GGAACTGCCT GCATGCCAAT GCTGGTCGTA CAAAAATCTG GGCTAACCAG 
GTGCCCGAGA TCTTCATGCA AACCTATGCA TCTGACAGTT TCTTCCTGAA CCTTGGAGCA 
GCCTTACTCC GCCTCTGCCA ACCATTCTCT AAGCCACGTT CTGCACGTCT GCTGACTTTT 
AACCCCACCT ATTGTGCTCT AAAAGAAGTC AATGAGGAGG AACGCCGGAG CAGGAATATG 
CACATGAAAG GGTTGGATAA GGAGACCTGC TTGATACCAG CTGCTGCTGA CCAGCAGCCT 
GACTTTCCTG AGAACTTCAA CTTGGTGACA GAAAACCTAG TGTTGACACA ATACACATTG 
CACTTAGGCT TCCATAGGTT GCATGAGCAA ATGGTTAAGG TCAACCAGAG CCTGCATCGG 
CTTCAGGGTG CATGGAGAGA TGCCCAGCAG AGTGGAAGCC CCACAGCAGA GAACCTTAGA 
GAACAGTTTG AGCGGCTTAT GACCATTTAC CTATGTCTGA AAACAGCTTT GAGTGAACCC 
CAAATGCTTC AGAACTGTAT TCATCTTCAA GTGTCAACTG CACTGCTCTT AGTGCAAATT 
GCATATGGCA ACAAAGGCAC AGAACCAGTG GCCCTCTCTT TTCCTATGCC TAATGTACAG 
CACAGTGCTC TGGCTTATGT ACCAGAATTC TTTGCTGATA ATTTGGGGGA TTTCTTCATA 
TTCCTACGCC GTTTTGCAGA TGAAGTTTTG GAAACTGCAG CAGACTCCTT AGAGCAAATT 
CTTGATTTTA TCACTGTTTT TACTGGCAGT GTTGAGAGAA TGAAGAACCC CCACTTGCGG 
GCCAAGCTGG CAGAGGTCCT AGAGGCAGTG ATGCCACATC TGGAACAGGT GCAAAACCCC 
CTGATTTCTA GTGTGTTCCA TCGGCAGCGT ATCTTCTGTT CATACCAACA TGCCCCTCAC 
TTGGCGGAAG CCCTCATTAA GGTGTTTGTT GACATTGAAT TCACAGGGGA CCCTCATCAG 
TTTGAACAGA AGTTTAATTA TCGGCGACCA ATGTATCCCA TACTAAAGTA CATGTGGGGA 
AGAGACAATT ACAGACAAAG TATAAAGAAC CTAGCTGACT ATGCCTCTAA AAATCTGGAA 
GCAGTGAACC CACCTCTCTT TTTACGGTTC CTTAATCTCC TCATGAATGA TGCTGTATTT 
CTGCTTGATG AGGCAATCCA GTATCTGAGC AAGATTAAAG TTCTGCAGAT AGAAAGGGAC 
CGAGGTGAAT GGGATGCATT GAGCCCTGAG AGCCGGCGGG AAAAGGAGTC CAACCTCCTT 
ATGTTTGGCC AGCTGGCCCG ATTTCATAAT ATCATGTCGA ATGAGACCAT TGGAACCTTG 
GCCTTCCTTA CCTCAGATAT ACGTTCTCTC TTTGTTCAGC CATTTCTTGC TGATCGCACT 
ATTTCCATGC TCAACTACTT CCTGCAGCAT CTTGTCGGGC CTAAGATGGG GGCCCTGAAG 
GTGAAAGATT TCAGTGAGTT TGACTTCAAG CCACAGCAAT TGGTGTCTGA CATCTGCACA 
ATCTACCTAA ATCTCGGGGA AGAGGAGCAT TTCTGTGCCT CTGTGCCTAA AGACGGACGC 
TCATATTCAC CCATGCTTTT TGCTCAAACT GTGCGGGTAC TAAAAAAGAT AAATAAGCCT 
GGAAATATGA TCGTGGCATT CACCAATTTA GCTGAACAGA TCAAGTCTTT GGCAGACCGG 
CAGCAACAGG AAGAAGAAAC ATTTGCTGAT GCCCCAGATG ACTTCTTGGA CCCTATTATG 
AGTACGGTCA TGTCTGACCC AGTAATTCTA CCCTCCTCCC GTGTTACTGT GGATAGGTCA 
ACTATCGCCC GGCACCTTCT GAGTGACCAG ACTGATCCTT TCAACCGAAG TCCTCTAACC 
ATGGATCAGA TCAAACCAAA TGTTGAATTA AAAGACAGGA TCCTTCAATG GCTAGCCCAG 
CGGAAGCAGC AGAGTGAGAA CCTTGGCCCC GGATCATTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822472.1
CDS	81..3302
Translation	MTDQENNNSFSANPFAALFGSLAEARQFVAGQKEQLRQTEDDAEESQDESDNSVSESLDDCDDSVAEISRSFRSQQEICEQLNINHMIQRIFLITLDNSDPSLKSGNGIPLRCVYLEELGSELDGQDWLDMDNVEQALFTRLLLPEPGKYLICMTSSGAPNLSAERDAGEKQIIPFLFSCFRRAKKEVTKVPENLLPYAVRCKALTVSNARTVLLTPEIYHGQDIATQLVDLMLEELRGANFEEVKEFLEEVIEALLKDEEVRSFSEVFHPVFDVLLERVQDLDLCQMLLYTYLDLLLYCTHQKDIAKVFVEYIQPQDPTNGHQYQKTLLGVILNISCLLRTPGVVENHGFFINPSRSSPQEIKVQESNIHQFMAQFHEKIYQLLKNLLQLSPDTKHRILSWLGNCLHANAGRTKIWANQVPEIFMQTYASDSFFLNLGAALLRLCQPFSKPRSARLLTFNPTYCALKEVNEEERRSRNMHMKGLDKETCLIPAAADQQPDFPENFNLVTENLVLTQYTLHLGFHRLHEQMVKVNQSLHRLQGAWRDAQQSGSPTAENLREQFERLMTIYLCLKTALSEPQMLQNCIHLQVSTALLLVQIAYGNKGTEPVALSFPMPNVQHSALAYVPEFFADNLGDFFIFLRRFADEVLETAADSLEQILDFITVFTGSVERMKNPHLRAKLAEVLEAVMPHLEQVQNPLISSVFHRQRIFCSYQHAPHLAEALIKVFVDIEFTGDPHQFEQKFNYRRPMYPILKYMWGRDNYRQSIKNLADYASKNLEAVNPPLFLRFLNLLMNDAVFLLDEAIQYLSKIKVLQIERDRGEWDALSPESRREKESNLLMFGQLARFHNIMSNETIGTLAFLTSDIRSLFVQPFLADRTISMLNYFLQHLVGPKMGALKVKDFSEFDFKPQQLVSDICTIYLNLGEEEHFCASVPKDGRSYSPMLFAQTVRVLKKINKPGNMIVAFTNLAEQIKSLADRQQQEEETFADAPDDFLDPIMSTVMSDPVILPSSRVTVDRSTIARHLLSDQTDPFNRSPLTMDQIKPNVELKDRILQWLAQRKQQSENLGPGSL

Target ORF information:

RefSeq Version	XM_012967018.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitination factor E4A (ube4a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967018.2

ORF Insert Sequence:

ATGACGGACC AAGAAAACAA CAACAGCTTT TCAGCCAATC CTTTTGCTGC CCTGTTTGGG 
TCTTTGGCTG AGGCTCGCCA ATTTGTGGCT GGTCAGAAAG AGCAACTCAG GCAGACTGAG 
GATGATGCTG AAGAGAGCCA AGATGAGTCA GACAACAGCG TGTCAGAAAG CCTGGATGAT 
TGTGATGACT CTGTGGCAGA AATCAGTCGA TCTTTTCGGT CCCAGCAGGA AATCTGTGAG 
CAGCTAAACA TTAATCACAT GATCCAAAGG ATCTTCCTCA TTACCTTGGA CAACAGTGAT 
CCCAGTCTGA AGAGTGGGAA TGGAATTCCT TTACGTTGTG TGTATTTGGA AGAGCTTGGA 
TCAGAGCTGG ATGGGCAGGA CTGGCTTGAC ATGGATAATG TTGAACAGGC TCTGTTTACA 
CGACTACTTC TCCCAGAGCC AGGAAAGTAT CTAATCTGTA TGACATCCTC TGGAGCCCCC 
AATTTATCTG CAGAGAGAGA TGCTGGAGAG AAACAAATAA TCCCATTCTT ATTTTCCTGT 
TTTCGTAGAG CCAAGAAAGA GGTCACAAAA GTGCCTGAGA ACCTTTTACC ATATGCTGTA 
CGCTGTAAAG CTCTGACTGT ATCCAATGCT CGTACTGTTC TACTGACCCC AGAGATATAC 
CATGGGCAGG ATATTGCCAC ACAGTTGGTG GACTTGATGC TAGAAGAACT CAGAGGAGCA 
AATTTTGAAG AGGTAAAGGA GTTCTTGGAG GAGGTGATAG AGGCTCTTCT GAAAGATGAG 
GAGGTCCGCT CTTTTAGTGA GGTCTTCCAC CCAGTATTCG ATGTTCTCCT GGAGCGCGTA 
CAGGATTTGG ACCTTTGTCA GATGCTACTC TACACTTACC TTGACCTTCT TCTATACTGC 
ACCCACCAGA AGGACATTGC CAAGGTGTTT GTGGAATATA TTCAGCCACA GGACCCCACA 
AACGGGCATC AGTATCAGAA GACTCTGTTG GGTGTAATCC TTAATATCTC TTGTTTACTG 
CGCACGCCAG GGGTAGTTGA GAATCATGGC TTCTTTATAA ATCCATCTCG CTCCAGCCCT 
CAAGAGATCA AAGTACAGGA ATCTAATATA CATCAGTTTA TGGCTCAGTT TCATGAGAAG 
ATTTATCAAC TTCTGAAGAA CCTACTTCAG TTATCCCCAG ATACTAAGCA CCGTATTTTG 
TCCTGGCTGG GGAACTGCCT GCATGCCAAT GCTGGTCGTA CAAAAATCTG GGCTAACCAG 
GTGCCCGAGA TCTTCATGCA AACCTATGCA TCTGACAGTT TCTTCCTGAA CCTTGGAGCA 
GCCTTACTCC GCCTCTGCCA ACCATTCTCT AAGCCACGTT CTGCACGTCT GCTGACTTTT 
AACCCCACCT ATTGTGCTCT AAAAGAAGTC AATGAGGAGG AACGCCGGAG CAGGAATATG 
CACATGAAAG GGTTGGATAA GGAGACCTGC TTGATACCAG CTGCTGCTGA CCAGCAGCCT 
GACTTTCCTG AGAACTTCAA CTTGGTGACA GAAAACCTAG TGTTGACACA ATACACATTG 
CACTTAGGCT TCCATAGGTT GCATGAGCAA ATGGTTAAGG TCAACCAGAG CCTGCATCGG 
CTTCAGGGTG CATGGAGAGA TGCCCAGCAG AGTGGAAGCC CCACAGCAGA GAACCTTAGA 
GAACAGTTTG AGCGGCTTAT GACCATTTAC CTATGTCTGA AAACAGCTTT GAGTGAACCC 
CAAATGCTTC AGAACTGTAT TCATCTTCAA GTGTCAACTG CACTGCTCTT AGTGCAAATT 
GCATATGGCA ACAAAGGCAC AGAACCAGTG GCCCTCTCTT TTCCTATGCC TAATGTACAG 
CACAGTGCTC TGGCTTATGT ACCAGAATTC TTTGCTGATA ATTTGGGGGA TTTCTTCATA 
TTCCTACGCC GTTTTGCAGA TGAAGTTTTG GAAACTGCAG CAGACTCCTT AGAGCAAATT 
CTTGATTTTA TCACTGTTTT TACTGGCAGT GTTGAGAGAA TGAAGAACCC CCACTTGCGG 
GCCAAGCTGG CAGAGGTCCT AGAGGCAGTG ATGCCACATC TGGAACAGGT GCAAAACCCC 
CTGATTTCTA GTGTGTTCCA TCGGCAGCGT ATCTTCTGTT CATACCAACA TGCCCCTCAC 
TTGGCGGAAG CCCTCATTAA GGTGTTTGTT GACATTGAAT TCACAGGGGA CCCTCATCAG 
TTTGAACAGA AGTTTAATTA TCGGCGACCA ATGTATCCCA TACTAAAGTA CATGTGGGGA 
AGAGACAATT ACAGACAAAG TATAAAGAAC CTAGCTGACT ATGCCTCTAA AAATCTGGAA 
GCAGTGAACC CACCTCTCTT TTTACGGTTC CTTAATCTCC TCATGAATGA TGCTGTATTT 
CTGCTTGATG AGGCAATCCA GTATCTGAGC AAGATTAAAG TTCTGCAGAT AGAAAGGGAC 
CGAGGTGAAT GGGATGCATT GAGCCCTGAG AGCCGGCGGG AAAAGGAGTC CAACCTCCTT 
ATGTTTGGCC AGCTGGCCCG ATTTCATAAT ATCATGTCGA ATGAGACCAT TGGAACCTTG 
GCCTTCCTTA CCTCAGATAT ACGTTCTCTC TTTGTTCAGC CATTTCTTGC TGATCGCACT 
ATTTCCATGC TCAACTACTT CCTGCAGCAT CTTGTCGGGC CTAAGATGGG GGCCCTGAAG 
GTGAAAGATT TCAGTGAGTT TGACTTCAAG CCACAGCAAT TGGTGTCTGA CATCTGCACA 
ATCTACCTAA ATCTCGGGGA AGAGGAGCAT TTCTGTGCCT CTGTGCCTAA AGACGGACGC 
TCATATTCAC CCATGCTTTT TGCTCAAACT GTGCGGGTAC TAAAAAAGAT AAATAAGCCT 
GGAAATATGA TCGTGGCATT CACCAATTTA GCTGAACAGA TCAAGTCTTT GGCAGACCGG 
CAGCAACAGG AAGAAGAAAC ATTTGCTGAT GCCCCAGATG ACTTCTTGGA CCCTATTATG 
AGTACGGTCA TGTCTGACCC AGTAATTCTA CCCTCCTCCC GTGTTACTGT GGATAGGTCA 
ACTATCGCCC GGCACCTTCT GAGTGACCAG ACTGATCCTT TCAACCGAAG TCCTCTAACC 
ATGGATCAGA TCAAACCAAA TGTTGAATTA AAAGACAGGA TCCTTCAATG GCTAGCCCAG 
CGGAAGCAGC AGAGTGAGAA CCTTGGCCCC GGATCATTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ube4a ( XM_018096066.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951555.1 Xenopus tropicalis ubiquitination factor E4A (ube4a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa45914
Clone ID Related Accession (Same CDS sequence)	XM_018096066.1
Accession Version	XM_018096066.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ubiquitin conjugation factor E4 A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGACC AAGAAAACAA CAACAGCTTT TCAGCCAATC CTTTTGCTGC CCTGTTTGGG 
TCTTTGGCTG AGGCTCGCCA ATTTGTGGCT GGTCAGAAAG AGCAACTCAG GCAGACTGAG 
GATGATGCTG AAGAGAGCCA AGATGAGTCA GACAACAGCG TGTCAGAAAG CCTGGATGAT 
TGTGATGACT CTGTGGCAGA AATCAGTCGA TCTTTTCGGT CCCAGCAGGA AATCTGTGAG 
CAGCTAAACA TTAATCACAT GATCCAAAGG ATCTTCCTCA TTACCTTGGA CAACAGTGAT 
CCCAGTCTGA AGAGTGGGAA TGGAATTCCT TTACGTTGTG TGTATTTGGA AGAGCTTGGA 
TCAGAGCTGG ATGGGCAGGA CTGGCTTGAC ATGGATAATG TTGAACAGGC TCTGTTTACA 
CGACTACTTC TCCCAGAGCC AGGAAAGTAT CTAATCTGTA TGACATCCTC TGGAGCCCCC 
AATTTATCTG CAGAGAGAGA TGCTGGAGAG AAACAAATAA TCCCATTCTT ATTTTCCTGT 
TTTCGTAGAG CCAAGAAAGA GGTCACAAAA GTGCCTGAGA ACCTTTTACC ATATGCTGTA 
CGCTGTAAAG CTCTGACTGT ATCCAATGCT CGTACTGTTC TACTGACCCC AGAGATATAC 
CATGGGCAGG ATATTGCCAC ACAGTTGGTG GACTTGATGC TAGAAGAACT CAGAGGAGCA 
AATTTTGAAG AGGTAAAGGA GTTCTTGGAG GAGGTGATAG AGGCTCTTCT GAAAGATGAG 
GAGGTCCGCT CTTTTAGTGA GGTCTTCCAC CCAGTATTCG ATGTTCTCCT GGAGCGCGTA 
CAGGATTTGG ACCTTTGTCA GATGCTACTC TACACTTACC TTGACCTTCT TCTATACTGC 
ACCCACCAGA AGGACATTGC CAAGGTGTTT GTGGAATATA TTCAGCCACA GGACCCCACA 
AACGGGCATC AGTATCAGAA GACTCTGTTG GGTGTAATCC TTAATATCTC TTGTTTACTG 
CGCACGCCAG GGGTAGTTGA GAATCATGGC TTCTTTATAA ATCCATCTCG CTCCAGCCCT 
CAAGAGATCA AAGTACAGGA ATCTAATATA CATCAGTTTA TGGCTCAGTT TCATGAGAAG 
ATTTATCAAC TTCTGAAGAA CCTACTTCAG TTATCCCCAG ATACTAAGCA CCGTATTTTG 
TCCTGGCTGG GGAACTGCCT GCATGCCAAT GCTGGTCGTA CAAAAATCTG GGCTAACCAG 
GTGCCCGAGA TCTTCATGCA AACCTATGCA TCTGACAGTT TCTTCCTGAA CCTTGGAGCA 
GCCTTACTCC GCCTCTGCCA ACCATTCTCT AAGCCACGTT CTGCACGTCT GCTGACTTTT 
AACCCCACCT ATTGTGCTCT AAAAGAAGTC AATGAGGAGG AACGCCGGAG CAGGAATATG 
CACATGAAAG GGTTGGATAA GGAGACCTGC TTGATACCAG CTGCTGCTGA CCAGCAGCCT 
GACTTTCCTG AGAACTTCAA CTTGGTGACA GAAAACCTAG TGTTGACACA ATACACATTG 
CACTTAGGCT TCCATAGGTT GCATGAGCAA ATGGTTAAGG TCAACCAGAG CCTGCATCGG 
CTTCAGGGTG CATGGAGAGA TGCCCAGCAG AGTGGAAGCC CCACAGCAGA GAACCTTAGA 
GAACAGTTTG AGCGGCTTAT GACCATTTAC CTATGTCTGA AAACAGCTTT GAGTGAACCC 
CAAATGCTTC AGAACTGTAT TCATCTTCAA GTGTCAACTG CACTGCTCTT AGTGCAAATT 
GCATATGGCA ACAAAGGCAC AGAACCAGTG GCCCTCTCTT TTCCTATGCC TAATGTACAG 
CACAGTGCTC TGGCTTATGT ACCAGAATTC TTTGCTGATA ATTTGGGGGA TTTCTTCATA 
TTCCTACGCC GTTTTGCAGA TGAAGTTTTG GAAACTGCAG CAGACTCCTT AGAGCAAATT 
CTTGATTTTA TCACTGTTTT TACTGGCAGT GTTGAGAGAA TGAAGAACCC CCACTTGCGG 
GCCAAGCTGG CAGAGGTCCT AGAGGCAGTG ATGCCACATC TGGAACAGGT GCAAAACCCC 
CTGATTTCTA GTGTGTTCCA TCGGCAGCGT ATCTTCTGTT CATACCAACA TGCCCCTCAC 
TTGGCGGAAG CCCTCATTAA GGTGTTTGTT GACATTGAAT TCACAGGGGA CCCTCATCAG 
TTTGAACAGA AGTTTAATTA TCGGCGACCA ATGTATCCCA TACTAAAGTA CATGTGGGGA 
AGAGACAATT ACAGACAAAG TATAAAGTAT CTGAGCAAGA TTAAAGTTCT GCAGATAGAA 
AGGGACCGAG GTGAATGGGA TGCATTGAGC CCTGAGAGCC GGCGGGAAAA GGAGTCCAAC 
CTCCTTATGT TTGGCCAGCT GGCCCGATTT CATAATATCA TGTCGAATGA GACCATTGGA 
ACCTTGGCCT TCCTTACCTC AGATATACGT TCTCTCTTTG TTCAGCCATT TCTTGCTGAT 
CGCACTATTT CCATGCTCAA CTACTTCCTG CAGCATCTTG TCGGGCCTAA GATGGGGGCC 
CTGAAGGTGA AAGATTTCAG TGAGTTTGAC TTCAAGCCAC AGCAATTGGT GTCTGACATC 
TGCACAATCT ACCTAAATCT CGGGGAAGAG GAGCATTTCT GTGCCTCTGT GCCTAAAGAC 
GGACGCTCAT ATTCACCCAT GCTTTTTGCT CAAACTGTGC GGGTACTAAA AAAGATAAAT 
AAGCCTGGAA ATATGATCGT GGCATTCACC AATTTAGCTG AACAGATCAA GTCTTTGGCA 
GACCGGCAGC AACAGGAAGA AGAAACATTT GCTGATGCCC CAGATGACTT CTTGGACCCT 
ATTATGAGTA CGGTCATGTC TGACCCAGTA ATTCTACCCT CCTCCCGTGT TACTGTGGAT 
AGGTCAACTA TCGCCCGGCA CCTTCTGAGT GACCAGACTG ATCCTTTCAA CCGAAGTCCT 
CTAACCATGG ATCAGATCAA ACCAAATGTT GAATTAAAAG ACAGGATCCT TCAATGGCTA 
GCCCAGCGGA AGCAGCAGAG TGAGAACCTT GGCCCCGGAT CATTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951555.1
CDS	1014..4121
Translation	MTDQENNNSFSANPFAALFGSLAEARQFVAGQKEQLRQTEDDAEESQDESDNSVSESLDDCDDSVAEISRSFRSQQEICEQLNINHMIQRIFLITLDNSDPSLKSGNGIPLRCVYLEELGSELDGQDWLDMDNVEQALFTRLLLPEPGKYLICMTSSGAPNLSAERDAGEKQIIPFLFSCFRRAKKEVTKVPENLLPYAVRCKALTVSNARTVLLTPEIYHGQDIATQLVDLMLEELRGANFEEVKEFLEEVIEALLKDEEVRSFSEVFHPVFDVLLERVQDLDLCQMLLYTYLDLLLYCTHQKDIAKVFVEYIQPQDPTNGHQYQKTLLGVILNISCLLRTPGVVENHGFFINPSRSSPQEIKVQESNIHQFMAQFHEKIYQLLKNLLQLSPDTKHRILSWLGNCLHANAGRTKIWANQVPEIFMQTYASDSFFLNLGAALLRLCQPFSKPRSARLLTFNPTYCALKEVNEEERRSRNMHMKGLDKETCLIPAAADQQPDFPENFNLVTENLVLTQYTLHLGFHRLHEQMVKVNQSLHRLQGAWRDAQQSGSPTAENLREQFERLMTIYLCLKTALSEPQMLQNCIHLQVSTALLLVQIAYGNKGTEPVALSFPMPNVQHSALAYVPEFFADNLGDFFIFLRRFADEVLETAADSLEQILDFITVFTGSVERMKNPHLRAKLAEVLEAVMPHLEQVQNPLISSVFHRQRIFCSYQHAPHLAEALIKVFVDIEFTGDPHQFEQKFNYRRPMYPILKYMWGRDNYRQSIKYLSKIKVLQIERDRGEWDALSPESRREKESNLLMFGQLARFHNIMSNETIGTLAFLTSDIRSLFVQPFLADRTISMLNYFLQHLVGPKMGALKVKDFSEFDFKPQQLVSDICTIYLNLGEEEHFCASVPKDGRSYSPMLFAQTVRVLKKINKPGNMIVAFTNLAEQIKSLADRQQQEEETFADAPDDFLDPIMSTVMSDPVILPSSRVTVDRSTIARHLLSDQTDPFNRSPLTMDQIKPNVELKDRILQWLAQRKQQSENLGPGSL

Target ORF information:

RefSeq Version	XM_018096066.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitination factor E4A (ube4a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096066.1

ORF Insert Sequence:

ATGACGGACC AAGAAAACAA CAACAGCTTT TCAGCCAATC CTTTTGCTGC CCTGTTTGGG 
TCTTTGGCTG AGGCTCGCCA ATTTGTGGCT GGTCAGAAAG AGCAACTCAG GCAGACTGAG 
GATGATGCTG AAGAGAGCCA AGATGAGTCA GACAACAGCG TGTCAGAAAG CCTGGATGAT 
TGTGATGACT CTGTGGCAGA AATCAGTCGA TCTTTTCGGT CCCAGCAGGA AATCTGTGAG 
CAGCTAAACA TTAATCACAT GATCCAAAGG ATCTTCCTCA TTACCTTGGA CAACAGTGAT 
CCCAGTCTGA AGAGTGGGAA TGGAATTCCT TTACGTTGTG TGTATTTGGA AGAGCTTGGA 
TCAGAGCTGG ATGGGCAGGA CTGGCTTGAC ATGGATAATG TTGAACAGGC TCTGTTTACA 
CGACTACTTC TCCCAGAGCC AGGAAAGTAT CTAATCTGTA TGACATCCTC TGGAGCCCCC 
AATTTATCTG CAGAGAGAGA TGCTGGAGAG AAACAAATAA TCCCATTCTT ATTTTCCTGT 
TTTCGTAGAG CCAAGAAAGA GGTCACAAAA GTGCCTGAGA ACCTTTTACC ATATGCTGTA 
CGCTGTAAAG CTCTGACTGT ATCCAATGCT CGTACTGTTC TACTGACCCC AGAGATATAC 
CATGGGCAGG ATATTGCCAC ACAGTTGGTG GACTTGATGC TAGAAGAACT CAGAGGAGCA 
AATTTTGAAG AGGTAAAGGA GTTCTTGGAG GAGGTGATAG AGGCTCTTCT GAAAGATGAG 
GAGGTCCGCT CTTTTAGTGA GGTCTTCCAC CCAGTATTCG ATGTTCTCCT GGAGCGCGTA 
CAGGATTTGG ACCTTTGTCA GATGCTACTC TACACTTACC TTGACCTTCT TCTATACTGC 
ACCCACCAGA AGGACATTGC CAAGGTGTTT GTGGAATATA TTCAGCCACA GGACCCCACA 
AACGGGCATC AGTATCAGAA GACTCTGTTG GGTGTAATCC TTAATATCTC TTGTTTACTG 
CGCACGCCAG GGGTAGTTGA GAATCATGGC TTCTTTATAA ATCCATCTCG CTCCAGCCCT 
CAAGAGATCA AAGTACAGGA ATCTAATATA CATCAGTTTA TGGCTCAGTT TCATGAGAAG 
ATTTATCAAC TTCTGAAGAA CCTACTTCAG TTATCCCCAG ATACTAAGCA CCGTATTTTG 
TCCTGGCTGG GGAACTGCCT GCATGCCAAT GCTGGTCGTA CAAAAATCTG GGCTAACCAG 
GTGCCCGAGA TCTTCATGCA AACCTATGCA TCTGACAGTT TCTTCCTGAA CCTTGGAGCA 
GCCTTACTCC GCCTCTGCCA ACCATTCTCT AAGCCACGTT CTGCACGTCT GCTGACTTTT 
AACCCCACCT ATTGTGCTCT AAAAGAAGTC AATGAGGAGG AACGCCGGAG CAGGAATATG 
CACATGAAAG GGTTGGATAA GGAGACCTGC TTGATACCAG CTGCTGCTGA CCAGCAGCCT 
GACTTTCCTG AGAACTTCAA CTTGGTGACA GAAAACCTAG TGTTGACACA ATACACATTG 
CACTTAGGCT TCCATAGGTT GCATGAGCAA ATGGTTAAGG TCAACCAGAG CCTGCATCGG 
CTTCAGGGTG CATGGAGAGA TGCCCAGCAG AGTGGAAGCC CCACAGCAGA GAACCTTAGA 
GAACAGTTTG AGCGGCTTAT GACCATTTAC CTATGTCTGA AAACAGCTTT GAGTGAACCC 
CAAATGCTTC AGAACTGTAT TCATCTTCAA GTGTCAACTG CACTGCTCTT AGTGCAAATT 
GCATATGGCA ACAAAGGCAC AGAACCAGTG GCCCTCTCTT TTCCTATGCC TAATGTACAG 
CACAGTGCTC TGGCTTATGT ACCAGAATTC TTTGCTGATA ATTTGGGGGA TTTCTTCATA 
TTCCTACGCC GTTTTGCAGA TGAAGTTTTG GAAACTGCAG CAGACTCCTT AGAGCAAATT 
CTTGATTTTA TCACTGTTTT TACTGGCAGT GTTGAGAGAA TGAAGAACCC CCACTTGCGG 
GCCAAGCTGG CAGAGGTCCT AGAGGCAGTG ATGCCACATC TGGAACAGGT GCAAAACCCC 
CTGATTTCTA GTGTGTTCCA TCGGCAGCGT ATCTTCTGTT CATACCAACA TGCCCCTCAC 
TTGGCGGAAG CCCTCATTAA GGTGTTTGTT GACATTGAAT TCACAGGGGA CCCTCATCAG 
TTTGAACAGA AGTTTAATTA TCGGCGACCA ATGTATCCCA TACTAAAGTA CATGTGGGGA 
AGAGACAATT ACAGACAAAG TATAAAGTAT CTGAGCAAGA TTAAAGTTCT GCAGATAGAA 
AGGGACCGAG GTGAATGGGA TGCATTGAGC CCTGAGAGCC GGCGGGAAAA GGAGTCCAAC 
CTCCTTATGT TTGGCCAGCT GGCCCGATTT CATAATATCA TGTCGAATGA GACCATTGGA 
ACCTTGGCCT TCCTTACCTC AGATATACGT TCTCTCTTTG TTCAGCCATT TCTTGCTGAT 
CGCACTATTT CCATGCTCAA CTACTTCCTG CAGCATCTTG TCGGGCCTAA GATGGGGGCC 
CTGAAGGTGA AAGATTTCAG TGAGTTTGAC TTCAAGCCAC AGCAATTGGT GTCTGACATC 
TGCACAATCT ACCTAAATCT CGGGGAAGAG GAGCATTTCT GTGCCTCTGT GCCTAAAGAC 
GGACGCTCAT ATTCACCCAT GCTTTTTGCT CAAACTGTGC GGGTACTAAA AAAGATAAAT 
AAGCCTGGAA ATATGATCGT GGCATTCACC AATTTAGCTG AACAGATCAA GTCTTTGGCA 
GACCGGCAGC AACAGGAAGA AGAAACATTT GCTGATGCCC CAGATGACTT CTTGGACCCT 
ATTATGAGTA CGGTCATGTC TGACCCAGTA ATTCTACCCT CCTCCCGTGT TACTGTGGAT 
AGGTCAACTA TCGCCCGGCA CCTTCTGAGT GACCAGACTG ATCCTTTCAA CCGAAGTCCT 
CTAACCATGG ATCAGATCAA ACCAAATGTT GAATTAAAAG ACAGGATCCT TCAATGGCTA 
GCCCAGCGGA AGCAGCAGAG TGAGAACCTT GGCCCCGGAT CATTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ube4a ( XM_012967018.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822472.1 Xenopus tropicalis ubiquitination factor E4A (ube4a), transcript variant X1, mRNA.

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CloneID	OXa30243
Clone ID Related Accession (Same CDS sequence)	XM_012967018.1 , XM_012967018.2
Accession Version	XM_012967018.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ubiquitin conjugation factor E4 A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGACC AAGAAAACAA CAACAGCTTT TCAGCCAATC CTTTTGCTGC CCTGTTTGGG 
TCTTTGGCTG AGGCTCGCCA ATTTGTGGCT GGTCAGAAAG AGCAACTCAG GCAGACTGAG 
GATGATGCTG AAGAGAGCCA AGATGAGTCA GACAACAGCG TGTCAGAAAG CCTGGATGAT 
TGTGATGACT CTGTGGCAGA AATCAGTCGA TCTTTTCGGT CCCAGCAGGA AATCTGTGAG 
CAGCTAAACA TTAATCACAT GATCCAAAGG ATCTTCCTCA TTACCTTGGA CAACAGTGAT 
CCCAGTCTGA AGAGTGGGAA TGGAATTCCT TTACGTTGTG TGTATTTGGA AGAGCTTGGA 
TCAGAGCTGG ATGGGCAGGA CTGGCTTGAC ATGGATAATG TTGAACAGGC TCTGTTTACA 
CGACTACTTC TCCCAGAGCC AGGAAAGTAT CTAATCTGTA TGACATCCTC TGGAGCCCCC 
AATTTATCTG CAGAGAGAGA TGCTGGAGAG AAACAAATAA TCCCATTCTT ATTTTCCTGT 
TTTCGTAGAG CCAAGAAAGA GGTCACAAAA GTGCCTGAGA ACCTTTTACC ATATGCTGTA 
CGCTGTAAAG CTCTGACTGT ATCCAATGCT CGTACTGTTC TACTGACCCC AGAGATATAC 
CATGGGCAGG ATATTGCCAC ACAGTTGGTG GACTTGATGC TAGAAGAACT CAGAGGAGCA 
AATTTTGAAG AGGTAAAGGA GTTCTTGGAG GAGGTGATAG AGGCTCTTCT GAAAGATGAG 
GAGGTCCGCT CTTTTAGTGA GGTCTTCCAC CCAGTATTCG ATGTTCTCCT GGAGCGCGTA 
CAGGATTTGG ACCTTTGTCA GATGCTACTC TACACTTACC TTGACCTTCT TCTATACTGC 
ACCCACCAGA AGGACATTGC CAAGGTGTTT GTGGAATATA TTCAGCCACA GGACCCCACA 
AACGGGCATC AGTATCAGAA GACTCTGTTG GGTGTAATCC TTAATATCTC TTGTTTACTG 
CGCACGCCAG GGGTAGTTGA GAATCATGGC TTCTTTATAA ATCCATCTCG CTCCAGCCCT 
CAAGAGATCA AAGTACAGGA ATCTAATATA CATCAGTTTA TGGCTCAGTT TCATGAGAAG 
ATTTATCAAC TTCTGAAGAA CCTACTTCAG TTATCCCCAG ATACTAAGCA CCGTATTTTG 
TCCTGGCTGG GGAACTGCCT GCATGCCAAT GCTGGTCGTA CAAAAATCTG GGCTAACCAG 
GTGCCCGAGA TCTTCATGCA AACCTATGCA TCTGACAGTT TCTTCCTGAA CCTTGGAGCA 
GCCTTACTCC GCCTCTGCCA ACCATTCTCT AAGCCACGTT CTGCACGTCT GCTGACTTTT 
AACCCCACCT ATTGTGCTCT AAAAGAAGTC AATGAGGAGG AACGCCGGAG CAGGAATATG 
CACATGAAAG GGTTGGATAA GGAGACCTGC TTGATACCAG CTGCTGCTGA CCAGCAGCCT 
GACTTTCCTG AGAACTTCAA CTTGGTGACA GAAAACCTAG TGTTGACACA ATACACATTG 
CACTTAGGCT TCCATAGGTT GCATGAGCAA ATGGTTAAGG TCAACCAGAG CCTGCATCGG 
CTTCAGGGTG CATGGAGAGA TGCCCAGCAG AGTGGAAGCC CCACAGCAGA GAACCTTAGA 
GAACAGTTTG AGCGGCTTAT GACCATTTAC CTATGTCTGA AAACAGCTTT GAGTGAACCC 
CAAATGCTTC AGAACTGTAT TCATCTTCAA GTGTCAACTG CACTGCTCTT AGTGCAAATT 
GCATATGGCA ACAAAGGCAC AGAACCAGTG GCCCTCTCTT TTCCTATGCC TAATGTACAG 
CACAGTGCTC TGGCTTATGT ACCAGAATTC TTTGCTGATA ATTTGGGGGA TTTCTTCATA 
TTCCTACGCC GTTTTGCAGA TGAAGTTTTG GAAACTGCAG CAGACTCCTT AGAGCAAATT 
CTTGATTTTA TCACTGTTTT TACTGGCAGT GTTGAGAGAA TGAAGAACCC CCACTTGCGG 
GCCAAGCTGG CAGAGGTCCT AGAGGCAGTG ATGCCACATC TGGAACAGGT GCAAAACCCC 
CTGATTTCTA GTGTGTTCCA TCGGCAGCGT ATCTTCTGTT CATACCAACA TGCCCCTCAC 
TTGGCGGAAG CCCTCATTAA GGTGTTTGTT GACATTGAAT TCACAGGGGA CCCTCATCAG 
TTTGAACAGA AGTTTAATTA TCGGCGACCA ATGTATCCCA TACTAAAGTA CATGTGGGGA 
AGAGACAATT ACAGACAAAG TATAAAGAAC CTAGCTGACT ATGCCTCTAA AAATCTGGAA 
GCAGTGAACC CACCTCTCTT TTTACGGTTC CTTAATCTCC TCATGAATGA TGCTGTATTT 
CTGCTTGATG AGGCAATCCA GTATCTGAGC AAGATTAAAG TTCTGCAGAT AGAAAGGGAC 
CGAGGTGAAT GGGATGCATT GAGCCCTGAG AGCCGGCGGG AAAAGGAGTC CAACCTCCTT 
ATGTTTGGCC AGCTGGCCCG ATTTCATAAT ATCATGTCGA ATGAGACCAT TGGAACCTTG 
GCCTTCCTTA CCTCAGATAT ACGTTCTCTC TTTGTTCAGC CATTTCTTGC TGATCGCACT 
ATTTCCATGC TCAACTACTT CCTGCAGCAT CTTGTCGGGC CTAAGATGGG GGCCCTGAAG 
GTGAAAGATT TCAGTGAGTT TGACTTCAAG CCACAGCAAT TGGTGTCTGA CATCTGCACA 
ATCTACCTAA ATCTCGGGGA AGAGGAGCAT TTCTGTGCCT CTGTGCCTAA AGACGGACGC 
TCATATTCAC CCATGCTTTT TGCTCAAACT GTGCGGGTAC TAAAAAAGAT AAATAAGCCT 
GGAAATATGA TCGTGGCATT CACCAATTTA GCTGAACAGA TCAAGTCTTT GGCAGACCGG 
CAGCAACAGG AAGAAGAAAC ATTTGCTGAT GCCCCAGATG ACTTCTTGGA CCCTATTATG 
AGTACGGTCA TGTCTGACCC AGTAATTCTA CCCTCCTCCC GTGTTACTGT GGATAGGTCA 
ACTATCGCCC GGCACCTTCT GAGTGACCAG ACTGATCCTT TCAACCGAAG TCCTCTAACC 
ATGGATCAGA TCAAACCAAA TGTTGAATTA AAAGACAGGA TCCTTCAATG GCTAGCCCAG 
CGGAAGCAGC AGAGTGAGAA CCTTGGCCCC GGATCATTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822472.1
CDS	1014..4235
Translation	MTDQENNNSFSANPFAALFGSLAEARQFVAGQKEQLRQTEDDAEESQDESDNSVSESLDDCDDSVAEISRSFRSQQEICEQLNINHMIQRIFLITLDNSDPSLKSGNGIPLRCVYLEELGSELDGQDWLDMDNVEQALFTRLLLPEPGKYLICMTSSGAPNLSAERDAGEKQIIPFLFSCFRRAKKEVTKVPENLLPYAVRCKALTVSNARTVLLTPEIYHGQDIATQLVDLMLEELRGANFEEVKEFLEEVIEALLKDEEVRSFSEVFHPVFDVLLERVQDLDLCQMLLYTYLDLLLYCTHQKDIAKVFVEYIQPQDPTNGHQYQKTLLGVILNISCLLRTPGVVENHGFFINPSRSSPQEIKVQESNIHQFMAQFHEKIYQLLKNLLQLSPDTKHRILSWLGNCLHANAGRTKIWANQVPEIFMQTYASDSFFLNLGAALLRLCQPFSKPRSARLLTFNPTYCALKEVNEEERRSRNMHMKGLDKETCLIPAAADQQPDFPENFNLVTENLVLTQYTLHLGFHRLHEQMVKVNQSLHRLQGAWRDAQQSGSPTAENLREQFERLMTIYLCLKTALSEPQMLQNCIHLQVSTALLLVQIAYGNKGTEPVALSFPMPNVQHSALAYVPEFFADNLGDFFIFLRRFADEVLETAADSLEQILDFITVFTGSVERMKNPHLRAKLAEVLEAVMPHLEQVQNPLISSVFHRQRIFCSYQHAPHLAEALIKVFVDIEFTGDPHQFEQKFNYRRPMYPILKYMWGRDNYRQSIKNLADYASKNLEAVNPPLFLRFLNLLMNDAVFLLDEAIQYLSKIKVLQIERDRGEWDALSPESRREKESNLLMFGQLARFHNIMSNETIGTLAFLTSDIRSLFVQPFLADRTISMLNYFLQHLVGPKMGALKVKDFSEFDFKPQQLVSDICTIYLNLGEEEHFCASVPKDGRSYSPMLFAQTVRVLKKINKPGNMIVAFTNLAEQIKSLADRQQQEEETFADAPDDFLDPIMSTVMSDPVILPSSRVTVDRSTIARHLLSDQTDPFNRSPLTMDQIKPNVELKDRILQWLAQRKQQSENLGPGSL

Target ORF information:

RefSeq Version	XM_012967018.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitination factor E4A (ube4a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967018.1

ORF Insert Sequence:

ATGACGGACC AAGAAAACAA CAACAGCTTT TCAGCCAATC CTTTTGCTGC CCTGTTTGGG 
TCTTTGGCTG AGGCTCGCCA ATTTGTGGCT GGTCAGAAAG AGCAACTCAG GCAGACTGAG 
GATGATGCTG AAGAGAGCCA AGATGAGTCA GACAACAGCG TGTCAGAAAG CCTGGATGAT 
TGTGATGACT CTGTGGCAGA AATCAGTCGA TCTTTTCGGT CCCAGCAGGA AATCTGTGAG 
CAGCTAAACA TTAATCACAT GATCCAAAGG ATCTTCCTCA TTACCTTGGA CAACAGTGAT 
CCCAGTCTGA AGAGTGGGAA TGGAATTCCT TTACGTTGTG TGTATTTGGA AGAGCTTGGA 
TCAGAGCTGG ATGGGCAGGA CTGGCTTGAC ATGGATAATG TTGAACAGGC TCTGTTTACA 
CGACTACTTC TCCCAGAGCC AGGAAAGTAT CTAATCTGTA TGACATCCTC TGGAGCCCCC 
AATTTATCTG CAGAGAGAGA TGCTGGAGAG AAACAAATAA TCCCATTCTT ATTTTCCTGT 
TTTCGTAGAG CCAAGAAAGA GGTCACAAAA GTGCCTGAGA ACCTTTTACC ATATGCTGTA 
CGCTGTAAAG CTCTGACTGT ATCCAATGCT CGTACTGTTC TACTGACCCC AGAGATATAC 
CATGGGCAGG ATATTGCCAC ACAGTTGGTG GACTTGATGC TAGAAGAACT CAGAGGAGCA 
AATTTTGAAG AGGTAAAGGA GTTCTTGGAG GAGGTGATAG AGGCTCTTCT GAAAGATGAG 
GAGGTCCGCT CTTTTAGTGA GGTCTTCCAC CCAGTATTCG ATGTTCTCCT GGAGCGCGTA 
CAGGATTTGG ACCTTTGTCA GATGCTACTC TACACTTACC TTGACCTTCT TCTATACTGC 
ACCCACCAGA AGGACATTGC CAAGGTGTTT GTGGAATATA TTCAGCCACA GGACCCCACA 
AACGGGCATC AGTATCAGAA GACTCTGTTG GGTGTAATCC TTAATATCTC TTGTTTACTG 
CGCACGCCAG GGGTAGTTGA GAATCATGGC TTCTTTATAA ATCCATCTCG CTCCAGCCCT 
CAAGAGATCA AAGTACAGGA ATCTAATATA CATCAGTTTA TGGCTCAGTT TCATGAGAAG 
ATTTATCAAC TTCTGAAGAA CCTACTTCAG TTATCCCCAG ATACTAAGCA CCGTATTTTG 
TCCTGGCTGG GGAACTGCCT GCATGCCAAT GCTGGTCGTA CAAAAATCTG GGCTAACCAG 
GTGCCCGAGA TCTTCATGCA AACCTATGCA TCTGACAGTT TCTTCCTGAA CCTTGGAGCA 
GCCTTACTCC GCCTCTGCCA ACCATTCTCT AAGCCACGTT CTGCACGTCT GCTGACTTTT 
AACCCCACCT ATTGTGCTCT AAAAGAAGTC AATGAGGAGG AACGCCGGAG CAGGAATATG 
CACATGAAAG GGTTGGATAA GGAGACCTGC TTGATACCAG CTGCTGCTGA CCAGCAGCCT 
GACTTTCCTG AGAACTTCAA CTTGGTGACA GAAAACCTAG TGTTGACACA ATACACATTG 
CACTTAGGCT TCCATAGGTT GCATGAGCAA ATGGTTAAGG TCAACCAGAG CCTGCATCGG 
CTTCAGGGTG CATGGAGAGA TGCCCAGCAG AGTGGAAGCC CCACAGCAGA GAACCTTAGA 
GAACAGTTTG AGCGGCTTAT GACCATTTAC CTATGTCTGA AAACAGCTTT GAGTGAACCC 
CAAATGCTTC AGAACTGTAT TCATCTTCAA GTGTCAACTG CACTGCTCTT AGTGCAAATT 
GCATATGGCA ACAAAGGCAC AGAACCAGTG GCCCTCTCTT TTCCTATGCC TAATGTACAG 
CACAGTGCTC TGGCTTATGT ACCAGAATTC TTTGCTGATA ATTTGGGGGA TTTCTTCATA 
TTCCTACGCC GTTTTGCAGA TGAAGTTTTG GAAACTGCAG CAGACTCCTT AGAGCAAATT 
CTTGATTTTA TCACTGTTTT TACTGGCAGT GTTGAGAGAA TGAAGAACCC CCACTTGCGG 
GCCAAGCTGG CAGAGGTCCT AGAGGCAGTG ATGCCACATC TGGAACAGGT GCAAAACCCC 
CTGATTTCTA GTGTGTTCCA TCGGCAGCGT ATCTTCTGTT CATACCAACA TGCCCCTCAC 
TTGGCGGAAG CCCTCATTAA GGTGTTTGTT GACATTGAAT TCACAGGGGA CCCTCATCAG 
TTTGAACAGA AGTTTAATTA TCGGCGACCA ATGTATCCCA TACTAAAGTA CATGTGGGGA 
AGAGACAATT ACAGACAAAG TATAAAGAAC CTAGCTGACT ATGCCTCTAA AAATCTGGAA 
GCAGTGAACC CACCTCTCTT TTTACGGTTC CTTAATCTCC TCATGAATGA TGCTGTATTT 
CTGCTTGATG AGGCAATCCA GTATCTGAGC AAGATTAAAG TTCTGCAGAT AGAAAGGGAC 
CGAGGTGAAT GGGATGCATT GAGCCCTGAG AGCCGGCGGG AAAAGGAGTC CAACCTCCTT 
ATGTTTGGCC AGCTGGCCCG ATTTCATAAT ATCATGTCGA ATGAGACCAT TGGAACCTTG 
GCCTTCCTTA CCTCAGATAT ACGTTCTCTC TTTGTTCAGC CATTTCTTGC TGATCGCACT 
ATTTCCATGC TCAACTACTT CCTGCAGCAT CTTGTCGGGC CTAAGATGGG GGCCCTGAAG 
GTGAAAGATT TCAGTGAGTT TGACTTCAAG CCACAGCAAT TGGTGTCTGA CATCTGCACA 
ATCTACCTAA ATCTCGGGGA AGAGGAGCAT TTCTGTGCCT CTGTGCCTAA AGACGGACGC 
TCATATTCAC CCATGCTTTT TGCTCAAACT GTGCGGGTAC TAAAAAAGAT AAATAAGCCT 
GGAAATATGA TCGTGGCATT CACCAATTTA GCTGAACAGA TCAAGTCTTT GGCAGACCGG 
CAGCAACAGG AAGAAGAAAC ATTTGCTGAT GCCCCAGATG ACTTCTTGGA CCCTATTATG 
AGTACGGTCA TGTCTGACCC AGTAATTCTA CCCTCCTCCC GTGTTACTGT GGATAGGTCA 
ACTATCGCCC GGCACCTTCT GAGTGACCAG ACTGATCCTT TCAACCGAAG TCCTCTAACC 
ATGGATCAGA TCAAACCAAA TGTTGAATTA AAAGACAGGA TCCTTCAATG GCTAGCCCAG 
CGGAAGCAGC AGAGTGAGAA CCTTGGCCCC GGATCATTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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ube4a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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