col5a1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following col5a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the col5a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa14033 XM_004916645.4
Latest version!
Xenopus tropicalis collagen, type V, alpha 1 (col5a1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa30187 XM_012969175.3
Latest version!
Xenopus tropicalis collagen, type V, alpha 1 (col5a1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa30188 XM_012969176.3
Latest version!
Xenopus tropicalis collagen, type V, alpha 1 (col5a1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
View Old Accession Versions >>
OXa30188 XM_012969176.2 Xenopus tropicalis collagen, type V, alpha 1 (col5a1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OXa14033 XM_004916645.3 Xenopus tropicalis collagen, type V, alpha 1 (col5a1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OXa30187 XM_012969175.2 Xenopus tropicalis collagen, type V, alpha 1 (col5a1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa14033
    Clone ID Related Accession (Same CDS sequence) XM_004916645.3 , XM_004916645.4
    Accession Version XM_004916645.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5598bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(V) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004916645.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGAGTATGG ATTCCCACAC ACGATGGAAA AGACGGAATT GGACGACAGA GTGGCCCCTC 
    CTTGTAGCTT TAGCCTTGTC TATATGTTCC CGGTTCAGCG CAGCTGAACC GGCGGACCTG
    TTGAAGGCCT TAGAATTTCA CAATTTACCA GACGGTATCA AAAAAACGAC GGGCTTCTGC
    GCCTACCGCA AATCTTCAGA TGGACCAGAC GTGGCATTTA GAGTGTCCAA AGAGGCCCAG
    CTTAGCGCTC CGACCAATCA ATTGTTCTCC GATGTCGCAT TCCCAGAAGA TTTCTCCTTG
    TTGGCAACTG TGAAACCCAA AAAAGGAAGC CATTCCTACC TGCTCTCCAT CTACAATGAG
    CAAGGAACCC TGCAGATCGG CCTGACGCTG GGCTGGGAAC CGATGTTCGT CTATGAAGAC
    CACCAGGGAC ACCCACCCCC CGAGGATTAC CCCATCTTTA GAAACATTAA TCTGTCAGAT
    GGGAAGTGGC ACCGGGTAGC TTTCAGCGTG CACAAGAAGA CGATCACCCT GTTCCTGGAC
    TGCGTCAAGA AGGCCACCCT GCCCTTGAAA AGAAGCGACG GCCCCATTAT AGACACGAAC
    GGCATCGTCG TGTTCGGCAC CAGGATCCTG GACGAGCAAG TCTTTGAGGG AGACATTCAA
    CAGCTGATGA TCGTGCCCGA CGCCCGTGCT GCACATGATT ATTGTGAACA CTACACCCCC
    GACTGTGACA CCGCCATTCC AGATGCACCG CAGTCTCAGG ATCCCAACCA AGATGAATAT
    TACCCAGAAG CTGCTGAAGC CGAAGGCGAG GGTTATTACT ATGAATATCC TTACTACGAA
    GATGAGAAGA CAGAAACTGC AACTACCAAG CCCACGCCTG AGAAAGCCGA GGTACTAAGG
    CCCGAGACAG AAATCGAAGA GGAGGAAACA CCCCCACCAA CTGAAGCCCC TGCTATTCCC
    GTCACTGATG CTCCACGATT TGAGACCAGG GAAGAGAGCA GGGTAGAGAG CAGGGTAGAG
    AGCAGAGTGG ATAACCCCAC CACCGATGAC TACAACTATG AAACCGTACA GGAAGAATAT
    TACACCGCCT CTCCTTATGA AGATGTCAAC TACTCTGAAG GAGAGGAAAC AGGAATTGAG
    ACTGGGACTA CTGGTGAAGG CCTCATCGAG GGAGAAGGAG AAATTTTGAC CAAAACTGTC
    ATCACAAGCA GCTCATCCAA TGGCTCATCG GTCATCAGCA GTGGAAGTTC CAAAAAAGAA
    GGCAGTACCG ACGGAGATTT CACAGAAGTG ACCATACAGG AGTACGACCA AGGGAAAGAT
    ACTTACTATG ACATCTACGA TGATAAGCTT CTCACTGACA CTTCAGACGG AATTGGTGGG
    CAGCCAGCTG AACAAGATCA ATTTGGCATT TCAGGTGGGG CCAGAGGTGA GAAAGGACAG
    AAGGGAGAGC CAGCCATCAT TGAACCCGGA ATGCTGATCG AAGGACCACC AGGCCCAGAA
    GGTCCAGCTG GTTATCCCGG TCCTCCTGGT ACCTCAGGTC CTCCTGGTCC AGCTGGAGAT
    CCTGGAGAGA GGGGCCCCCC TGGTCGTGCA GGTCTTCCTG GAGCTGATGG CTTGCCCGGA
    CCACCAGGAA CCATGCTGAT GTTGCCCTTC CGCTTCTCTG GTGGTGGAGA TGGTGGATCT
    AAAGGACCCC AAGTGTCTGC CCAGGAATCA CAAGCCCAAG CTATTCTCCA ACAAGCAAGA
    CTGGCGTTGA GAGGGCCATC TGGTCCGATG GGTCTTACTG GAAGACCAGG CCCATTGGGT
    TCTCCAGGTC CTTCAGGATT AAAGGGAGAG TCAGGAGATT TGGGACCTCA GGGGCCACGT
    GGGCCACAAG GTTCTCCCGG TCCATTAGGA AAACCAGGAC GAAGGGGACG AGCTGGTAGT
    GATGGTGCTC GGGGAATGCC TGGACAAACA GGACCAAAGG GTGACCGAGG CTTTGACGGG
    TTGGGCGGTT TGCCAGGAGA AAAGGGACAT CGTGGTGAGC CTGGTCCACA AGGTCCACCA
    GGGCCACATG GAGAAGACGG AGAAAGGGGT GATGATGGAG ACGTTGGACC TAGAGGGTTA
    CCAGGTGAAC CTGGACCCCG AGGGTTGCTT GGACCTAAAG GACCTCCTGG CCCACCGGGA
    ATCACCGGTG TTCCTGGAAT GGACGGTCAA GTTGGACCCA AAGGTAGTGT GGGACCTCAA
    GGTGAGCCAG GACCACCTGG GCAGCAAGGA AACCCAGGAG CACAGGGTCT TCCAGGTCCT
    CAGGGAGCTA TTGGACCACC AGGAGAGAAG GGACCACTGG GAAAATCAGG TCTTCCCGGA
    ATGCCCGGAG CTGATGGACC TCCCGGTCAC CCCGGGAAAG AAGGACCCTC AGGAGAGAAA
    GGAACCCTGG GTCCTGCAGG GCCACAGGGA CCAATTGGAT ACCCAGGACC ACGTGGTGTT
    AAGGGAGCTG ATGGAATTCG TGGTCTAAAG GGAACAAAAG GAGAAAAGGG TGAAGATGGA
    TTCCCTGGAT TTAAGGGTGA TATGGGCACC AAAGGAGACA GAGGTGAAAT CGGTCCTCCT
    GGTCCTAGAG GTGAAGATGG TCCTGAAGGT CCAAAGGGTC GAGGTGGTCC ATCTGGAGAA
    ATAGGTCCAC TTGGTCCTGG CGGTGAAAAG GGAAAGCTTG GTGTTCCTGG TCTACCAGGT
    TATCCAGGAA GACAAGGTCC AAAGGGTTCT ATCGGATTCC CAGGATTCGC AGGAGCAAAC
    GGAGAGAAAG GAACAAGGGG TACCCCTGGC AAACCGGGTC CAAGGGGGCA ACGAGGTCCA
    ACGGGTCCAC GTGGTGAAAG AGGTCCAAGG GGAGCTACAG GCAAGCCAGG CCCCAAGGGT
    AACTCCGGAG GGGATGGCCC AGCAGGTCCA CCTGGTGAAA GGGGACCACC TGGACCTCAA
    GGACCCACTG GATTTGCAGG ACCGAAGGGC CCCCCTGGTC CACCAGGGAA AGATGGACTT
    CCCGGCCACC CTGGACAGAG AGGAGAGACT GGATTCCAAG GAAAGACTGG CCCTCCTGGT
    CCCCCTGGCG TTGTCGGTCC TCAGGGTCCA ACTGGAGAGA CTGGCCCAAT GGGTGAAAGA
    GGACATCCTG GTCCTCCAGG TCCTCCTGGT GAGCAAGGTT TACCTGGTGT GGCTGGAAAA
    GAAGGATCCA AGGGAGACCC CGGTCCTGCT GGTTTGCCTG GAAAAGATGG ACCTCCAGGT
    CTAAGAGGTT TTACTGGAGA AAGAGGTCTC CCCGGTCCGA TTGGTGTTCC TGGACTGAAA
    GGCAATGAGG GTCCTCCTGG TCCACCTGGC CCAGCTGGTT CCCCCGGTGA AAGAGGAGCT
    GCGGGTCCAG CCGGACCTAT TGGACTGCCT GGAAGACCTG GCCCTCAGGG GCCACCAGGA
    CCTGCCGGCG AGAAGGGTAC ACCAGGTGAG AAAGGTCCCC AGGGACCTGC TGGACGTGAT
    GGTATCCAAG GCCCCGTTGG ACTTCCAGGC CCAGCTGGAC CTGTAGGGCC ACCTGGTGAA
    GATGGTGATA AGGGTGAAAT CGGAGAGCCT GGGCAAAAAG GAAACAGAGG AGATAAAGGA
    GAGCAGGGTC CCCCAGGTCC ACCTGGCCCA CAAGGTCCCA TTGGTCAGCC TGGCCCATCA
    GGATCTGATG GAGAGCCAGG CCCCAGGGGT CAACAAGGTC TTTTTGGACA AAAAGGTGAT
    GAAGGACCTA GAGGTTTTGC TGGACCACCA GGACCTGTTG GACTGCAGGG TTTACCTGGA
    CCACCTGGTG AGAAGGGAGA AACCGGAGAC GTTGGTCAGA TGGGTCCTCC TGGTCCCCCT
    GGTCCTAGAG GTCCTTCTGG CTCCCCTGGA GCTGATGGCC CACAAGGTCC TCCTGGAGGA
    ATTGGCAACC CTGGTCCTGT AGGAGAGAAG GGTGACCCTG GAGAGTCTGG GGAACCTGGT
    CTTCCTGGTG AACTTGGACC ACCGGGCCCT AAAGGTGAAA GAGGTGAAAA GGGAGAAGCG
    GGTCTTCAAG GAACTTCTGG CCCTCCAGGT CCTAAAGGTC CTCCAGGTGA TGATGGTCCA
    AAAGGTAGCC CTGGCCCAGT TGGATTTCCT GGTGATCCTG GTCCTCCTGG AGAATCTGGT
    CCTGCAGGTC AAGATGGTCC ACCTGGTGAT AAGGGAGAGG ACGGTGAATC TGGACAGCCA
    GGGTCACCAG GTCCTACTGG AGAGCCTGGG CCTTCAGGAC CACCTGGAAA GAGGGGTCCT
    CCTGGTCCAA CAGGTCCTGA AGGACGTCAA GGAGAAAAAG GAGCAAAGGG TGAATCAGGT
    TTGGAAGGAC CTCCTGGTAA GACTGGTCCT ATTGGTCCCC AAGGACCTCC CGGAAAACCT
    GGACCTGAAG GCCTTCGAGG AATTCCAGGC CCTGTTGGTG AACAAGGTCT CCCTGGTTCA
    CCTGGACCTG ATGGCCCTCC CGGACCTATG GGTCCACCAG GTATACCAGG TCTGAAAGGA
    GACTCAGGTC CAAAAGGAGA AAAGGGTCAT CCAGGCTTGA TAGGTCTCAT TGGACCTCCT
    GGTGAACAGG GAGAAAAGGG AGACCGTGGT TTGCCAGGCC CCCAGGGACC ACATGGTGCT
    AAAGGAGAAC AGGGTATTCT TGGTCCATCT GGTCCTGTTG GTCCACCTGG TCCTCCAGGC
    TTACCGGGTG GTCCTGGTCC AAAAGGTGCT AAAGGTTCAT CGGGTCCAAC TGGTCCAAAG
    GGTGAATCTG GAATCCCTGG TCCACCAGGT CCTCCAGGAC CTCCAGGCGA GGTGATCCAG
    CCTCTGCCTA TCCATTCCTC AAAGAGGACA AGACGTAGCA TTGATGCAAG TCAAGTAGTG
    GACGATGGAA ATGCAGACAA CTACATGGAC TATGCCGATG GAATGGAGGA AATATTTGGT
    TCTCTCAACT CCCTAAAACA GGAAATTGAA CAAATGAAAC ACCCACTGGG CAATCAGCAG
    AACCCAGCAA GGACTTGCAA GGACCTGCTT CTGTGCCATC CTGAACTCCC AGATGGGGAA
    TATTGGATTG ACCCAAATCA AGGATGCTCT AGAGACTCGT TCAAGGTTTT CTGTAATTTC
    ACAGCCGGTG GAGAATCTTG TATCTTCCCC GACAAGAAGT CTGAAGGAGC TAGAATTACA
    TCTTGGCCCA AGGAAAATCC AGGCTCCTGG TTCAGTGAAT TCAAGCGTGG TAAACTGTTG
    TCCTACGTTG ATTCAAATGG CAACCCCATT GGAGTGGTGC AGATGACCTT CATGAAGCTT
    CTGAGTGCTT CGGTGAAACA GAATATCACC TACAACTGCT ACCAGTCCGT GGCATGGCAC
    GACGCCACCA CCAACAGCTA CGACAAGGCC ATTCGCTTCC TGGGGTCCAA CGACGAAGAA
    ATGTCCTACG ACAACAATCC TTCCATTCGA GCCATTATGG ATGGATGTGC GTCAAAGAAA
    GGTTATCAGA AGACAGTTCT GGAACTCGAC ACGTCCAAAG TGGAACAGGC CCCCATTGTG
    GATATTATGT TCAACGACTT CGGAGATCCA GTACAAAAAT TCGGCTTTGA GGTGGGACCA
    GTGTGTTTCA TGGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004916702.1
    CDS343..5940
    Translation

    Target ORF information:

    RefSeq Version XM_004916645.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type V, alpha 1 (col5a1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004916645.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGAGTATGG ATTCCCACAC ACGATGGAAA AGACGGAATT GGACGACAGA GTGGCCCCTC 
    CTTGTAGCTT TAGCCTTGTC TATATGTTCC CGGTTCAGCG CAGCTGAACC GGCGGACCTG
    TTGAAGGCCT TAGAATTTCA CAATTTACCA GACGGTATCA AAAAAACGAC GGGCTTCTGC
    GCCTACCGCA AATCTTCAGA TGGACCAGAC GTGGCATTTA GAGTGTCCAA AGAGGCCCAG
    CTTAGCGCTC CGACCAATCA ATTGTTCTCC GATGTCGCAT TCCCAGAAGA TTTCTCCTTG
    TTGGCAACTG TGAAACCCAA AAAAGGAAGC CATTCCTACC TGCTCTCCAT CTACAATGAG
    CAAGGAACCC TGCAGATCGG CCTGACGCTG GGCTGGGAAC CGATGTTCGT CTATGAAGAC
    CACCAGGGAC ACCCACCCCC CGAGGATTAC CCCATCTTTA GAAACATTAA TCTGTCAGAT
    GGGAAGTGGC ACCGGGTAGC TTTCAGCGTG CACAAGAAGA CGATCACCCT GTTCCTGGAC
    TGCGTCAAGA AGGCCACCCT GCCCTTGAAA AGAAGCGACG GCCCCATTAT AGACACGAAC
    GGCATCGTCG TGTTCGGCAC CAGGATCCTG GACGAGCAAG TCTTTGAGGG AGACATTCAA
    CAGCTGATGA TCGTGCCCGA CGCCCGTGCT GCACATGATT ATTGTGAACA CTACACCCCC
    GACTGTGACA CCGCCATTCC AGATGCACCG CAGTCTCAGG ATCCCAACCA AGATGAATAT
    TACCCAGAAG CTGCTGAAGC CGAAGGCGAG GGTTATTACT ATGAATATCC TTACTACGAA
    GATGAGAAGA CAGAAACTGC AACTACCAAG CCCACGCCTG AGAAAGCCGA GGTACTAAGG
    CCCGAGACAG AAATCGAAGA GGAGGAAACA CCCCCACCAA CTGAAGCCCC TGCTATTCCC
    GTCACTGATG CTCCACGATT TGAGACCAGG GAAGAGAGCA GGGTAGAGAG CAGGGTAGAG
    AGCAGAGTGG ATAACCCCAC CACCGATGAC TACAACTATG AAACCGTACA GGAAGAATAT
    TACACCGCCT CTCCTTATGA AGATGTCAAC TACTCTGAAG GAGAGGAAAC AGGAATTGAG
    ACTGGGACTA CTGGTGAAGG CCTCATCGAG GGAGAAGGAG AAATTTTGAC CAAAACTGTC
    ATCACAAGCA GCTCATCCAA TGGCTCATCG GTCATCAGCA GTGGAAGTTC CAAAAAAGAA
    GGCAGTACCG ACGGAGATTT CACAGAAGTG ACCATACAGG AGTACGACCA AGGGAAAGAT
    ACTTACTATG ACATCTACGA TGATAAGCTT CTCACTGACA CTTCAGACGG AATTGGTGGG
    CAGCCAGCTG AACAAGATCA ATTTGGCATT TCAGGTGGGG CCAGAGGTGA GAAAGGACAG
    AAGGGAGAGC CAGCCATCAT TGAACCCGGA ATGCTGATCG AAGGACCACC AGGCCCAGAA
    GGTCCAGCTG GTTATCCCGG TCCTCCTGGT ACCTCAGGTC CTCCTGGTCC AGCTGGAGAT
    CCTGGAGAGA GGGGCCCCCC TGGTCGTGCA GGTCTTCCTG GAGCTGATGG CTTGCCCGGA
    CCACCAGGAA CCATGCTGAT GTTGCCCTTC CGCTTCTCTG GTGGTGGAGA TGGTGGATCT
    AAAGGACCCC AAGTGTCTGC CCAGGAATCA CAAGCCCAAG CTATTCTCCA ACAAGCAAGA
    CTGGCGTTGA GAGGGCCATC TGGTCCGATG GGTCTTACTG GAAGACCAGG CCCATTGGGT
    TCTCCAGGTC CTTCAGGATT AAAGGGAGAG TCAGGAGATT TGGGACCTCA GGGGCCACGT
    GGGCCACAAG GTTCTCCCGG TCCATTAGGA AAACCAGGAC GAAGGGGACG AGCTGGTAGT
    GATGGTGCTC GGGGAATGCC TGGACAAACA GGACCAAAGG GTGACCGAGG CTTTGACGGG
    TTGGGCGGTT TGCCAGGAGA AAAGGGACAT CGTGGTGAGC CTGGTCCACA AGGTCCACCA
    GGGCCACATG GAGAAGACGG AGAAAGGGGT GATGATGGAG ACGTTGGACC TAGAGGGTTA
    CCAGGTGAAC CTGGACCCCG AGGGTTGCTT GGACCTAAAG GACCTCCTGG CCCACCGGGA
    ATCACCGGTG TTCCTGGAAT GGACGGTCAA GTTGGACCCA AAGGTAGTGT GGGACCTCAA
    GGTGAGCCAG GACCACCTGG GCAGCAAGGA AACCCAGGAG CACAGGGTCT TCCAGGTCCT
    CAGGGAGCTA TTGGACCACC AGGAGAGAAG GGACCACTGG GAAAATCAGG TCTTCCCGGA
    ATGCCCGGAG CTGATGGACC TCCCGGTCAC CCCGGGAAAG AAGGACCCTC AGGAGAGAAA
    GGAACCCTGG GTCCTGCAGG GCCACAGGGA CCAATTGGAT ACCCAGGACC ACGTGGTGTT
    AAGGGAGCTG ATGGAATTCG TGGTCTAAAG GGAACAAAAG GAGAAAAGGG TGAAGATGGA
    TTCCCTGGAT TTAAGGGTGA TATGGGCACC AAAGGAGACA GAGGTGAAAT CGGTCCTCCT
    GGTCCTAGAG GTGAAGATGG TCCTGAAGGT CCAAAGGGTC GAGGTGGTCC ATCTGGAGAA
    ATAGGTCCAC TTGGTCCTGG CGGTGAAAAG GGAAAGCTTG GTGTTCCTGG TCTACCAGGT
    TATCCAGGAA GACAAGGTCC AAAGGGTTCT ATCGGATTCC CAGGATTCGC AGGAGCAAAC
    GGAGAGAAAG GAACAAGGGG TACCCCTGGC AAACCGGGTC CAAGGGGGCA ACGAGGTCCA
    ACGGGTCCAC GTGGTGAAAG AGGTCCAAGG GGAGCTACAG GCAAGCCAGG CCCCAAGGGT
    AACTCCGGAG GGGATGGCCC AGCAGGTCCA CCTGGTGAAA GGGGACCACC TGGACCTCAA
    GGACCCACTG GATTTGCAGG ACCGAAGGGC CCCCCTGGTC CACCAGGGAA AGATGGACTT
    CCCGGCCACC CTGGACAGAG AGGAGAGACT GGATTCCAAG GAAAGACTGG CCCTCCTGGT
    CCCCCTGGCG TTGTCGGTCC TCAGGGTCCA ACTGGAGAGA CTGGCCCAAT GGGTGAAAGA
    GGACATCCTG GTCCTCCAGG TCCTCCTGGT GAGCAAGGTT TACCTGGTGT GGCTGGAAAA
    GAAGGATCCA AGGGAGACCC CGGTCCTGCT GGTTTGCCTG GAAAAGATGG ACCTCCAGGT
    CTAAGAGGTT TTACTGGAGA AAGAGGTCTC CCCGGTCCGA TTGGTGTTCC TGGACTGAAA
    GGCAATGAGG GTCCTCCTGG TCCACCTGGC CCAGCTGGTT CCCCCGGTGA AAGAGGAGCT
    GCGGGTCCAG CCGGACCTAT TGGACTGCCT GGAAGACCTG GCCCTCAGGG GCCACCAGGA
    CCTGCCGGCG AGAAGGGTAC ACCAGGTGAG AAAGGTCCCC AGGGACCTGC TGGACGTGAT
    GGTATCCAAG GCCCCGTTGG ACTTCCAGGC CCAGCTGGAC CTGTAGGGCC ACCTGGTGAA
    GATGGTGATA AGGGTGAAAT CGGAGAGCCT GGGCAAAAAG GAAACAGAGG AGATAAAGGA
    GAGCAGGGTC CCCCAGGTCC ACCTGGCCCA CAAGGTCCCA TTGGTCAGCC TGGCCCATCA
    GGATCTGATG GAGAGCCAGG CCCCAGGGGT CAACAAGGTC TTTTTGGACA AAAAGGTGAT
    GAAGGACCTA GAGGTTTTGC TGGACCACCA GGACCTGTTG GACTGCAGGG TTTACCTGGA
    CCACCTGGTG AGAAGGGAGA AACCGGAGAC GTTGGTCAGA TGGGTCCTCC TGGTCCCCCT
    GGTCCTAGAG GTCCTTCTGG CTCCCCTGGA GCTGATGGCC CACAAGGTCC TCCTGGAGGA
    ATTGGCAACC CTGGTCCTGT AGGAGAGAAG GGTGACCCTG GAGAGTCTGG GGAACCTGGT
    CTTCCTGGTG AACTTGGACC ACCGGGCCCT AAAGGTGAAA GAGGTGAAAA GGGAGAAGCG
    GGTCTTCAAG GAACTTCTGG CCCTCCAGGT CCTAAAGGTC CTCCAGGTGA TGATGGTCCA
    AAAGGTAGCC CTGGCCCAGT TGGATTTCCT GGTGATCCTG GTCCTCCTGG AGAATCTGGT
    CCTGCAGGTC AAGATGGTCC ACCTGGTGAT AAGGGAGAGG ACGGTGAATC TGGACAGCCA
    GGGTCACCAG GTCCTACTGG AGAGCCTGGG CCTTCAGGAC CACCTGGAAA GAGGGGTCCT
    CCTGGTCCAA CAGGTCCTGA AGGACGTCAA GGAGAAAAAG GAGCAAAGGG TGAATCAGGT
    TTGGAAGGAC CTCCTGGTAA GACTGGTCCT ATTGGTCCCC AAGGACCTCC CGGAAAACCT
    GGACCTGAAG GCCTTCGAGG AATTCCAGGC CCTGTTGGTG AACAAGGTCT CCCTGGTTCA
    CCTGGACCTG ATGGCCCTCC CGGACCTATG GGTCCACCAG GTATACCAGG TCTGAAAGGA
    GACTCAGGTC CAAAAGGAGA AAAGGGTCAT CCAGGCTTGA TAGGTCTCAT TGGACCTCCT
    GGTGAACAGG GAGAAAAGGG AGACCGTGGT TTGCCAGGCC CCCAGGGACC ACATGGTGCT
    AAAGGAGAAC AGGGTATTCT TGGTCCATCT GGTCCTGTTG GTCCACCTGG TCCTCCAGGC
    TTACCGGGTG GTCCTGGTCC AAAAGGTGCT AAAGGTTCAT CGGGTCCAAC TGGTCCAAAG
    GGTGAATCTG GAATCCCTGG TCCACCAGGT CCTCCAGGAC CTCCAGGCGA GGTGATCCAG
    CCTCTGCCTA TCCATTCCTC AAAGAGGACA AGACGTAGCA TTGATGCAAG TCAAGTAGTG
    GACGATGGAA ATGCAGACAA CTACATGGAC TATGCCGATG GAATGGAGGA AATATTTGGT
    TCTCTCAACT CCCTAAAACA GGAAATTGAA CAAATGAAAC ACCCACTGGG CAATCAGCAG
    AACCCAGCAA GGACTTGCAA GGACCTGCTT CTGTGCCATC CTGAACTCCC AGATGGGGAA
    TATTGGATTG ACCCAAATCA AGGATGCTCT AGAGACTCGT TCAAGGTTTT CTGTAATTTC
    ACAGCCGGTG GAGAATCTTG TATCTTCCCC GACAAGAAGT CTGAAGGAGC TAGAATTACA
    TCTTGGCCCA AGGAAAATCC AGGCTCCTGG TTCAGTGAAT TCAAGCGTGG TAAACTGTTG
    TCCTACGTTG ATTCAAATGG CAACCCCATT GGAGTGGTGC AGATGACCTT CATGAAGCTT
    CTGAGTGCTT CGGTGAAACA GAATATCACC TACAACTGCT ACCAGTCCGT GGCATGGCAC
    GACGCCACCA CCAACAGCTA CGACAAGGCC ATTCGCTTCC TGGGGTCCAA CGACGAAGAA
    ATGTCCTACG ACAACAATCC TTCCATTCGA GCCATTATGG ATGGATGTGC GTCAAAGAAA
    GGTTATCAGA AGACAGTTCT GGAACTCGAC ACGTCCAAAG TGGAACAGGC CCCCATTGTG
    GATATTATGT TCAACGACTT CGGAGATCCA GTACAAAAAT TCGGCTTTGA GGTGGGACCA
    GTGTGTTTCA TGGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30187
    Clone ID Related Accession (Same CDS sequence) XM_012969175.2 , XM_012969175.3
    Accession Version XM_012969175.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5598bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(V) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012969175.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGAGTATGG ATTCCCACAC ACGATGGAAA AGACGGAATT GGACGACAGA GTGGCCCCTC 
    CTTGTAGCTT TAGCCTTGTC TATATGTTCC CGGTTCAGCG CAGCTGAACC GGCGGACCTG
    TTGAAGGCCT TAGAATTTCA CAATTTACCA GACGGTATCA AAAAAACGAC GGGCTTCTGC
    GCCTACCGCA AATCTTCAGA TGGACCAGAC GTGGCATTTA GAGTGTCCAA AGAGGCCCAG
    CTTAGCGCTC CGACCAATCA ATTGTTCTCC GATGTCGCAT TCCCAGAAGA TTTCTCCTTG
    TTGGCAACTG TGAAACCCAA AAAAGGAAGC CATTCCTACC TGCTCTCCAT CTACAATGAG
    CAAGGAACCC TGCAGATCGG CCTGACGCTG GGCTGGGAAC CGATGTTCGT CTATGAAGAC
    CACCAGGGAC ACCCACCCCC CGAGGATTAC CCCATCTTTA GAAACATTAA TCTGTCAGAT
    GGGAAGTGGC ACCGGGTAGC TTTCAGCGTG CACAAGAAGA CGATCACCCT GTTCCTGGAC
    TGCGTCAAGA AGGCCACCCT GCCCTTGAAA AGAAGCGACG GCCCCATTAT AGACACGAAC
    GGCATCGTCG TGTTCGGCAC CAGGATCCTG GACGAGCAAG TCTTTGAGGG AGACATTCAA
    CAGCTGATGA TCGTGCCCGA CGCCCGTGCT GCACATGATT ATTGTGAACA CTACACCCCC
    GACTGTGACA CCGCCATTCC AGATGCACCG CAGTCTCAGG ATCCCAACCA AGATGAATAT
    TACCCAGAAG CTGCTGAAGC CGAAGGCGAG GGTTATTACT ATGAATATCC TTACTACGAA
    GATGAGAAGA CAGAAACTGC AACTACCAAG CCCACGCCTG AGAAAGCCGA GGTACTAAGG
    CCCGAGACAG AAATCGAAGA GGAGGAAACA CCCCCACCAA CTGAAGCCCC TGCTATTCCC
    GTCACTGATG CTCCACGATT TGAGACCAGG GAAGAGAGCA GGGTAGAGAG CAGGGTAGAG
    AGCAGAGTGG ATAACCCCAC CACCGATGAC TACAACTATG AAACCGTACA GGAAGAATAT
    TACACCGCCT CTCCTTATGA AGATGTCAAC TACTCTGAAG GAGAGGAAAC AGGAATTGAG
    ACTGGGACTA CTGGTGAAGG CCTCATCGAG GGAGAAGGAG AAATTTTGAC CAAAACTGTC
    ATCACAAGCA GCTCATCCAA TGGCTCATCG GTCATCAGCA GTGGAAGTTC CAAAAAAGAA
    GGCAGTACCG ACGGAGATTT CACAGAAGTG ACCATACAGG AGTACGACCA AGGGAAAGAT
    ACTTACTATG ACATCTACGA TGATAAGCTT CTCACTGACA CTTCAGACGG AATTGGTGGG
    CAGCCAGCTG AACAAGATCA ATTTGGCATT TCAGGTGGGG CCAGAGGTGA GAAAGGACAG
    AAGGGAGAGC CAGCCATCAT TGAACCCGGA ATGCTGATCG AAGGACCACC AGGCCCAGAA
    GGTCCAGCTG GTTATCCCGG TCCTCCTGGT ACCTCAGGTC CTCCTGGTCC AGCTGGAGAT
    CCTGGAGAGA GGGGCCCCCC TGGTCGTGCA GGTCTTCCTG GAGCTGATGG CTTGCCCGGA
    CCACCAGGAA CCATGCTGAT GTTGCCCTTC CGCTTCTCTG GTGGTGGAGA TGGTGGATCT
    AAAGGACCCC AAGTGTCTGC CCAGGAATCA CAAGCCCAAG CTATTCTCCA ACAAGCAAGA
    CTGGCGTTGA GAGGGCCATC TGGTCCGATG GGTCTTACTG GAAGACCAGG CCCATTGGGT
    TCTCCAGGTC CTTCAGGATT AAAGGGAGAG TCAGGAGATT TGGGACCTCA GGGGCCACGT
    GGGCCACAAG GTTCTCCCGG TCCATTAGGA AAACCAGGAC GAAGGGGACG AGCTGGTAGT
    GATGGTGCTC GGGGAATGCC TGGACAAACA GGACCAAAGG GTGACCGAGG CTTTGACGGG
    TTGGGCGGTT TGCCAGGAGA AAAGGGACAT CGTGGTGAGC CTGGTCCACA AGGTCCACCA
    GGGCCACATG GAGAAGACGG AGAAAGGGGT GATGATGGAG ACGTTGGACC TAGAGGGTTA
    CCAGGTGAAC CTGGACCCCG AGGGTTGCTT GGACCTAAAG GACCTCCTGG CCCACCGGGA
    ATCACCGGTG TTCCTGGAAT GGACGGTCAA GTTGGACCCA AAGGTAGTGT GGGACCTCAA
    GGTGAGCCAG GACCACCTGG GCAGCAAGGA AACCCAGGAG CACAGGGTCT TCCAGGTCCT
    CAGGGAGCTA TTGGACCACC AGGAGAGAAG GGACCACTGG GAAAATCAGG TCTTCCCGGA
    ATGCCCGGAG CTGATGGACC TCCCGGTCAC CCCGGGAAAG AAGGACCCTC AGGAGAGAAA
    GGAACCCTGG GTCCTGCAGG GCCACAGGGA CCAATTGGAT ACCCAGGACC ACGTGGTGTT
    AAGGGAGCTG ATGGAATTCG TGGTCTAAAG GGAACAAAAG GAGAAAAGGG TGAAGATGGA
    TTCCCTGGAT TTAAGGGTGA TATGGGCACC AAAGGAGACA GAGGTGAAAT CGGTCCTCCT
    GGTCCTAGAG GTGAAGATGG TCCTGAAGGT CCAAAGGGTC GAGGTGGTCC ATCTGGAGAA
    ATAGGTCCAC TTGGTCCTGG CGGTGAAAAG GGAAAGCTTG GTGTTCCTGG TCTACCAGGT
    TATCCAGGAA GACAAGGTCC AAAGGGTTCT ATCGGATTCC CAGGATTCGC AGGAGCAAAC
    GGAGAGAAAG GAACAAGGGG TACCCCTGGC AAACCGGGTC CAAGGGGGCA ACGAGGTCCA
    ACGGGTCCAC GTGGTGAAAG AGGTCCAAGG GGAGCTACAG GCAAGCCAGG CCCCAAGGGT
    AACTCCGGAG GGGATGGCCC AGCAGGTCCA CCTGGTGAAA GGGGACCACC TGGACCTCAA
    GGACCCACTG GATTTGCAGG ACCGAAGGGC CCCCCTGGTC CACCAGGGAA AGATGGACTT
    CCCGGCCACC CTGGACAGAG AGGAGAGACT GGATTCCAAG GAAAGACTGG CCCTCCTGGT
    CCCCCTGGCG TTGTCGGTCC TCAGGGTCCA ACTGGAGAGA CTGGCCCAAT GGGTGAAAGA
    GGACATCCTG GTCCTCCAGG TCCTCCTGGT GAGCAAGGTT TACCTGGTGT GGCTGGAAAA
    GAAGGATCCA AGGGAGACCC CGGTCCTGCT GGTTTGCCTG GAAAAGATGG ACCTCCAGGT
    CTAAGAGGTT TTACTGGAGA AAGAGGTCTC CCCGGTCCGA TTGGTGTTCC TGGACTGAAA
    GGCAATGAGG GTCCTCCTGG TCCACCTGGC CCAGCTGGTT CCCCCGGTGA AAGAGGAGCT
    GCGGGTCCAG CCGGACCTAT TGGACTGCCT GGAAGACCTG GCCCTCAGGG GCCACCAGGA
    CCTGCCGGCG AGAAGGGTAC ACCAGGTGAG AAAGGTCCCC AGGGACCTGC TGGACGTGAT
    GGTATCCAAG GCCCCGTTGG ACTTCCAGGC CCAGCTGGAC CTGTAGGGCC ACCTGGTGAA
    GATGGTGATA AGGGTGAAAT CGGAGAGCCT GGGCAAAAAG GAAACAGAGG AGATAAAGGA
    GAGCAGGGTC CCCCAGGTCC ACCTGGCCCA CAAGGTCCCA TTGGTCAGCC TGGCCCATCA
    GGATCTGATG GAGAGCCAGG CCCCAGGGGT CAACAAGGTC TTTTTGGACA AAAAGGTGAT
    GAAGGACCTA GAGGTTTTGC TGGACCACCA GGACCTGTTG GACTGCAGGG TTTACCTGGA
    CCACCTGGTG AGAAGGGAGA AACCGGAGAC GTTGGTCAGA TGGGTCCTCC TGGTCCCCCT
    GGTCCTAGAG GTCCTTCTGG CTCCCCTGGA GCTGATGGCC CACAAGGTCC TCCTGGAGGA
    ATTGGCAACC CTGGTCCTGT AGGAGAGAAG GGTGACCCTG GAGAGTCTGG GGAACCTGGT
    CTTCCTGGTG AACTTGGACC ACCGGGCCCT AAAGGTGAAA GAGGTGAAAA GGGAGAAGCG
    GGTCTTCAAG GAACTTCTGG CCCTCCAGGT CCTAAAGGTC CTCCAGGTGA TGATGGTCCA
    AAAGGTAGCC CTGGCCCAGT TGGATTTCCT GGTGATCCTG GTCCTCCTGG AGAATCTGGT
    CCTGCAGGTC AAGATGGTCC ACCTGGTGAT AAGGGAGAGG ACGGTGAATC TGGACAGCCA
    GGGTCACCAG GTCCTACTGG AGAGCCTGGG CCTTCAGGAC CACCTGGAAA GAGGGGTCCT
    CCTGGTCCAA CAGGTCCTGA AGGACGTCAA GGAGAAAAAG GAGCAAAGGG TGAATCAGGT
    TTGGAAGGAC CTCCTGGTAA GACTGGTCCT ATTGGTCCCC AAGGACCTCC CGGAAAACCT
    GGACCTGAAG GCCTTCGAGG AATTCCAGGC CCTGTTGGTG AACAAGGTCT CCCTGGTTCA
    CCTGGACCTG ATGGCCCTCC CGGACCTATG GGTCCACCAG GTATACCAGG TCTGAAAGGA
    GACTCAGGTC CAAAAGGAGA AAAGGGTCAT CCAGGCTTGA TAGGTCTCAT TGGACCTCCT
    GGTGAACAGG GAGAAAAGGG AGACCGTGGT TTGCCAGGCC CCCAGGGACC ACATGGTGCT
    AAAGGAGAAC AGGGTATTCT TGGTCCATCT GGTCCTGTTG GTCCACCTGG TCCTCCAGGC
    TTACCGGGTG GTCCTGGTCC AAAAGGTGCT AAAGGTTCAT CGGGTCCAAC TGGTCCAAAG
    GGTGAATCTG GAATCCCTGG TCCACCAGGT CCTCCAGGAC CTCCAGGCGA GGTGATCCAG
    CCTCTGCCTA TCCATTCCTC AAAGAGGACA AGACGTAGCA TTGATGCAAG TCAAGTAGTG
    GACGATGGAA ATGCAGACAA CTACATGGAC TATGCCGATG GAATGGAGGA AATATTTGGT
    TCTCTCAACT CCCTAAAACA GGAAATTGAA CAAATGAAAC ACCCACTGGG CAATCAGCAG
    AACCCAGCAA GGACTTGCAA GGACCTGCTT CTGTGCCATC CTGAACTCCC AGATGGGGAA
    TATTGGATTG ACCCAAATCA AGGATGCTCT AGAGACTCGT TCAAGGTTTT CTGTAATTTC
    ACAGCCGGTG GAGAATCTTG TATCTTCCCC GACAAGAAGT CTGAAGGAAA TAAACTGGCT
    CGTTGGCCCA AAGAGCTGCC TGGTACATGG TATAGTCATT ACAAGCGGGG TTCCATGTTG
    TCCTACGTTG ATTCAAATGG CAACCCCATT GGAGTGGTGC AGATGACCTT CATGAAGCTT
    CTGAGTGCTT CGGTGAAACA GAATATCACC TACAACTGCT ACCAGTCCGT GGCATGGCAC
    GACGCCACCA CCAACAGCTA CGACAAGGCC ATTCGCTTCC TGGGGTCCAA CGACGAAGAA
    ATGTCCTACG ACAACAATCC TTCCATTCGA GCCATTATGG ATGGATGTGC GTCAAAGAAA
    GGTTATCAGA AGACAGTTCT GGAACTCGAC ACGTCCAAAG TGGAACAGGC CCCCATTGTG
    GATATTATGT TCAACGACTT CGGAGATCCA GTACAAAAAT TCGGCTTTGA GGTGGGACCA
    GTGTGTTTCA TGGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012824629.1
    CDS343..5940
    Translation

    Target ORF information:

    RefSeq Version XM_012969175.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type V, alpha 1 (col5a1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012969175.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGAGTATGG ATTCCCACAC ACGATGGAAA AGACGGAATT GGACGACAGA GTGGCCCCTC 
    CTTGTAGCTT TAGCCTTGTC TATATGTTCC CGGTTCAGCG CAGCTGAACC GGCGGACCTG
    TTGAAGGCCT TAGAATTTCA CAATTTACCA GACGGTATCA AAAAAACGAC GGGCTTCTGC
    GCCTACCGCA AATCTTCAGA TGGACCAGAC GTGGCATTTA GAGTGTCCAA AGAGGCCCAG
    CTTAGCGCTC CGACCAATCA ATTGTTCTCC GATGTCGCAT TCCCAGAAGA TTTCTCCTTG
    TTGGCAACTG TGAAACCCAA AAAAGGAAGC CATTCCTACC TGCTCTCCAT CTACAATGAG
    CAAGGAACCC TGCAGATCGG CCTGACGCTG GGCTGGGAAC CGATGTTCGT CTATGAAGAC
    CACCAGGGAC ACCCACCCCC CGAGGATTAC CCCATCTTTA GAAACATTAA TCTGTCAGAT
    GGGAAGTGGC ACCGGGTAGC TTTCAGCGTG CACAAGAAGA CGATCACCCT GTTCCTGGAC
    TGCGTCAAGA AGGCCACCCT GCCCTTGAAA AGAAGCGACG GCCCCATTAT AGACACGAAC
    GGCATCGTCG TGTTCGGCAC CAGGATCCTG GACGAGCAAG TCTTTGAGGG AGACATTCAA
    CAGCTGATGA TCGTGCCCGA CGCCCGTGCT GCACATGATT ATTGTGAACA CTACACCCCC
    GACTGTGACA CCGCCATTCC AGATGCACCG CAGTCTCAGG ATCCCAACCA AGATGAATAT
    TACCCAGAAG CTGCTGAAGC CGAAGGCGAG GGTTATTACT ATGAATATCC TTACTACGAA
    GATGAGAAGA CAGAAACTGC AACTACCAAG CCCACGCCTG AGAAAGCCGA GGTACTAAGG
    CCCGAGACAG AAATCGAAGA GGAGGAAACA CCCCCACCAA CTGAAGCCCC TGCTATTCCC
    GTCACTGATG CTCCACGATT TGAGACCAGG GAAGAGAGCA GGGTAGAGAG CAGGGTAGAG
    AGCAGAGTGG ATAACCCCAC CACCGATGAC TACAACTATG AAACCGTACA GGAAGAATAT
    TACACCGCCT CTCCTTATGA AGATGTCAAC TACTCTGAAG GAGAGGAAAC AGGAATTGAG
    ACTGGGACTA CTGGTGAAGG CCTCATCGAG GGAGAAGGAG AAATTTTGAC CAAAACTGTC
    ATCACAAGCA GCTCATCCAA TGGCTCATCG GTCATCAGCA GTGGAAGTTC CAAAAAAGAA
    GGCAGTACCG ACGGAGATTT CACAGAAGTG ACCATACAGG AGTACGACCA AGGGAAAGAT
    ACTTACTATG ACATCTACGA TGATAAGCTT CTCACTGACA CTTCAGACGG AATTGGTGGG
    CAGCCAGCTG AACAAGATCA ATTTGGCATT TCAGGTGGGG CCAGAGGTGA GAAAGGACAG
    AAGGGAGAGC CAGCCATCAT TGAACCCGGA ATGCTGATCG AAGGACCACC AGGCCCAGAA
    GGTCCAGCTG GTTATCCCGG TCCTCCTGGT ACCTCAGGTC CTCCTGGTCC AGCTGGAGAT
    CCTGGAGAGA GGGGCCCCCC TGGTCGTGCA GGTCTTCCTG GAGCTGATGG CTTGCCCGGA
    CCACCAGGAA CCATGCTGAT GTTGCCCTTC CGCTTCTCTG GTGGTGGAGA TGGTGGATCT
    AAAGGACCCC AAGTGTCTGC CCAGGAATCA CAAGCCCAAG CTATTCTCCA ACAAGCAAGA
    CTGGCGTTGA GAGGGCCATC TGGTCCGATG GGTCTTACTG GAAGACCAGG CCCATTGGGT
    TCTCCAGGTC CTTCAGGATT AAAGGGAGAG TCAGGAGATT TGGGACCTCA GGGGCCACGT
    GGGCCACAAG GTTCTCCCGG TCCATTAGGA AAACCAGGAC GAAGGGGACG AGCTGGTAGT
    GATGGTGCTC GGGGAATGCC TGGACAAACA GGACCAAAGG GTGACCGAGG CTTTGACGGG
    TTGGGCGGTT TGCCAGGAGA AAAGGGACAT CGTGGTGAGC CTGGTCCACA AGGTCCACCA
    GGGCCACATG GAGAAGACGG AGAAAGGGGT GATGATGGAG ACGTTGGACC TAGAGGGTTA
    CCAGGTGAAC CTGGACCCCG AGGGTTGCTT GGACCTAAAG GACCTCCTGG CCCACCGGGA
    ATCACCGGTG TTCCTGGAAT GGACGGTCAA GTTGGACCCA AAGGTAGTGT GGGACCTCAA
    GGTGAGCCAG GACCACCTGG GCAGCAAGGA AACCCAGGAG CACAGGGTCT TCCAGGTCCT
    CAGGGAGCTA TTGGACCACC AGGAGAGAAG GGACCACTGG GAAAATCAGG TCTTCCCGGA
    ATGCCCGGAG CTGATGGACC TCCCGGTCAC CCCGGGAAAG AAGGACCCTC AGGAGAGAAA
    GGAACCCTGG GTCCTGCAGG GCCACAGGGA CCAATTGGAT ACCCAGGACC ACGTGGTGTT
    AAGGGAGCTG ATGGAATTCG TGGTCTAAAG GGAACAAAAG GAGAAAAGGG TGAAGATGGA
    TTCCCTGGAT TTAAGGGTGA TATGGGCACC AAAGGAGACA GAGGTGAAAT CGGTCCTCCT
    GGTCCTAGAG GTGAAGATGG TCCTGAAGGT CCAAAGGGTC GAGGTGGTCC ATCTGGAGAA
    ATAGGTCCAC TTGGTCCTGG CGGTGAAAAG GGAAAGCTTG GTGTTCCTGG TCTACCAGGT
    TATCCAGGAA GACAAGGTCC AAAGGGTTCT ATCGGATTCC CAGGATTCGC AGGAGCAAAC
    GGAGAGAAAG GAACAAGGGG TACCCCTGGC AAACCGGGTC CAAGGGGGCA ACGAGGTCCA
    ACGGGTCCAC GTGGTGAAAG AGGTCCAAGG GGAGCTACAG GCAAGCCAGG CCCCAAGGGT
    AACTCCGGAG GGGATGGCCC AGCAGGTCCA CCTGGTGAAA GGGGACCACC TGGACCTCAA
    GGACCCACTG GATTTGCAGG ACCGAAGGGC CCCCCTGGTC CACCAGGGAA AGATGGACTT
    CCCGGCCACC CTGGACAGAG AGGAGAGACT GGATTCCAAG GAAAGACTGG CCCTCCTGGT
    CCCCCTGGCG TTGTCGGTCC TCAGGGTCCA ACTGGAGAGA CTGGCCCAAT GGGTGAAAGA
    GGACATCCTG GTCCTCCAGG TCCTCCTGGT GAGCAAGGTT TACCTGGTGT GGCTGGAAAA
    GAAGGATCCA AGGGAGACCC CGGTCCTGCT GGTTTGCCTG GAAAAGATGG ACCTCCAGGT
    CTAAGAGGTT TTACTGGAGA AAGAGGTCTC CCCGGTCCGA TTGGTGTTCC TGGACTGAAA
    GGCAATGAGG GTCCTCCTGG TCCACCTGGC CCAGCTGGTT CCCCCGGTGA AAGAGGAGCT
    GCGGGTCCAG CCGGACCTAT TGGACTGCCT GGAAGACCTG GCCCTCAGGG GCCACCAGGA
    CCTGCCGGCG AGAAGGGTAC ACCAGGTGAG AAAGGTCCCC AGGGACCTGC TGGACGTGAT
    GGTATCCAAG GCCCCGTTGG ACTTCCAGGC CCAGCTGGAC CTGTAGGGCC ACCTGGTGAA
    GATGGTGATA AGGGTGAAAT CGGAGAGCCT GGGCAAAAAG GAAACAGAGG AGATAAAGGA
    GAGCAGGGTC CCCCAGGTCC ACCTGGCCCA CAAGGTCCCA TTGGTCAGCC TGGCCCATCA
    GGATCTGATG GAGAGCCAGG CCCCAGGGGT CAACAAGGTC TTTTTGGACA AAAAGGTGAT
    GAAGGACCTA GAGGTTTTGC TGGACCACCA GGACCTGTTG GACTGCAGGG TTTACCTGGA
    CCACCTGGTG AGAAGGGAGA AACCGGAGAC GTTGGTCAGA TGGGTCCTCC TGGTCCCCCT
    GGTCCTAGAG GTCCTTCTGG CTCCCCTGGA GCTGATGGCC CACAAGGTCC TCCTGGAGGA
    ATTGGCAACC CTGGTCCTGT AGGAGAGAAG GGTGACCCTG GAGAGTCTGG GGAACCTGGT
    CTTCCTGGTG AACTTGGACC ACCGGGCCCT AAAGGTGAAA GAGGTGAAAA GGGAGAAGCG
    GGTCTTCAAG GAACTTCTGG CCCTCCAGGT CCTAAAGGTC CTCCAGGTGA TGATGGTCCA
    AAAGGTAGCC CTGGCCCAGT TGGATTTCCT GGTGATCCTG GTCCTCCTGG AGAATCTGGT
    CCTGCAGGTC AAGATGGTCC ACCTGGTGAT AAGGGAGAGG ACGGTGAATC TGGACAGCCA
    GGGTCACCAG GTCCTACTGG AGAGCCTGGG CCTTCAGGAC CACCTGGAAA GAGGGGTCCT
    CCTGGTCCAA CAGGTCCTGA AGGACGTCAA GGAGAAAAAG GAGCAAAGGG TGAATCAGGT
    TTGGAAGGAC CTCCTGGTAA GACTGGTCCT ATTGGTCCCC AAGGACCTCC CGGAAAACCT
    GGACCTGAAG GCCTTCGAGG AATTCCAGGC CCTGTTGGTG AACAAGGTCT CCCTGGTTCA
    CCTGGACCTG ATGGCCCTCC CGGACCTATG GGTCCACCAG GTATACCAGG TCTGAAAGGA
    GACTCAGGTC CAAAAGGAGA AAAGGGTCAT CCAGGCTTGA TAGGTCTCAT TGGACCTCCT
    GGTGAACAGG GAGAAAAGGG AGACCGTGGT TTGCCAGGCC CCCAGGGACC ACATGGTGCT
    AAAGGAGAAC AGGGTATTCT TGGTCCATCT GGTCCTGTTG GTCCACCTGG TCCTCCAGGC
    TTACCGGGTG GTCCTGGTCC AAAAGGTGCT AAAGGTTCAT CGGGTCCAAC TGGTCCAAAG
    GGTGAATCTG GAATCCCTGG TCCACCAGGT CCTCCAGGAC CTCCAGGCGA GGTGATCCAG
    CCTCTGCCTA TCCATTCCTC AAAGAGGACA AGACGTAGCA TTGATGCAAG TCAAGTAGTG
    GACGATGGAA ATGCAGACAA CTACATGGAC TATGCCGATG GAATGGAGGA AATATTTGGT
    TCTCTCAACT CCCTAAAACA GGAAATTGAA CAAATGAAAC ACCCACTGGG CAATCAGCAG
    AACCCAGCAA GGACTTGCAA GGACCTGCTT CTGTGCCATC CTGAACTCCC AGATGGGGAA
    TATTGGATTG ACCCAAATCA AGGATGCTCT AGAGACTCGT TCAAGGTTTT CTGTAATTTC
    ACAGCCGGTG GAGAATCTTG TATCTTCCCC GACAAGAAGT CTGAAGGAAA TAAACTGGCT
    CGTTGGCCCA AAGAGCTGCC TGGTACATGG TATAGTCATT ACAAGCGGGG TTCCATGTTG
    TCCTACGTTG ATTCAAATGG CAACCCCATT GGAGTGGTGC AGATGACCTT CATGAAGCTT
    CTGAGTGCTT CGGTGAAACA GAATATCACC TACAACTGCT ACCAGTCCGT GGCATGGCAC
    GACGCCACCA CCAACAGCTA CGACAAGGCC ATTCGCTTCC TGGGGTCCAA CGACGAAGAA
    ATGTCCTACG ACAACAATCC TTCCATTCGA GCCATTATGG ATGGATGTGC GTCAAAGAAA
    GGTTATCAGA AGACAGTTCT GGAACTCGAC ACGTCCAAAG TGGAACAGGC CCCCATTGTG
    GATATTATGT TCAACGACTT CGGAGATCCA GTACAAAAAT TCGGCTTTGA GGTGGGACCA
    GTGTGTTTCA TGGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30188
    Clone ID Related Accession (Same CDS sequence) XM_012969176.2 , XM_012969176.3
    Accession Version XM_012969176.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4575bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(V) chain isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012969176.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTATGG ATTCCCACAC ACGATGGAAA AGACGGAATT GGACGACAGA GTGGCCCCTC 
    CTTGTAGCTT TAGCCTTGTC TATATGTTCC CGGTTCAGCG CAGCTGAACC GGCGGACCTG
    TTGAAGGCCT TAGAATTTCA CAATTTACCA GACGGTATCA AAAAAACGAC GGGCTTCTGC
    GCCTACCGCA AATCTTCAGA TGGACCAGAC GTGGCATTTA GAGTGTCCAA AGAGGCCCAG
    CTTAGCGCTC CGACCAATCA ATTGTTCTCC GATGTCGCAT TCCCAGAAGA TTTCTCCTTG
    TTGGCAACTG TGAAACCCAA AAAAGGAAGC CATTCCTACC TGCTCTCCAT CTACAATGAG
    CAAGGAACCC TGCAGATCGG CCTGACGCTG GGCTGGGAAC CGATGTTCGT CTATGAAGAC
    CACCAGGGAC ACCCACCCCC CGAGGATTAC CCCATCTTTA GAAACATTAA TCTGTCAGAT
    GGGAAGTGGC ACCGGGTAGC TTTCAGCGTG CACAAGAAGA CGATCACCCT GTTCCTGGAC
    TGCGTCAAGA AGGCCACCCT GCCCTTGAAA AGAAGCGACG GCCCCATTAT AGACACGAAC
    GGCATCGTCG TGTTCGGCAC CAGGATCCTG GACGAGCAAG TCTTTGAGGG AGACATTCAA
    CAGCTGATGA TCGTGCCCGA CGCCCGTGCT GCACATGATT ATTGTGAACA CTACACCCCC
    GACTGTGACA CCGCCATTCC AGATGCACCG CAGTCTCAGG ATCCCAACCA AGATGAATAT
    TACCCAGAAG CTGCTGAAGC CGAAGGCGAG GGTTATTACT ATGAATATCC TTACTACGAA
    GATGAGAAGA CAGAAACTGC AACTACCAAG CCCACGCCTG AGAAAGCCGA GGTACTAAGG
    CCCGAGACAG AAATCGAAGA GGAGGAAACA CCCCCACCAA CTGAAGCCCC TGCTATTCCC
    GTCACTGATG CTCCACGATT TGAGACCAGG GAAGAGAGCA GGGTAGAGAG CAGGGTAGAG
    AGCAGAGTGG ATAACCCCAC CACCGATGAC TACAACTATG AAACCGTACA GGAAGAATAT
    TACACCGCCT CTCCTTATGA AGATGTCAAC TACTCTGAAG GAGAGGAAAC AGGAATTGAG
    ACTGGGACTA CTGGTGAAGG CCTCATCGAG GGAGAAGGAG AAATTTTGAC CAAAACTGTC
    ATCACAAGCA GCTCATCCAA TGGCTCATCG GTCATCAGCA GTGGAAGTTC CAAAAAAGAA
    GGCAGTACCG ACGGAGATTT CACAGAAGTG ACCATACAGG AGTACGACCA AGGGAAAGAT
    ACTTACTATG ACATCTACGA TGATAAGCTT CTCACTGACA CTTCAGACGG AATTGGTGGG
    CAGCCAGCTG AACAAGATCA ATTTGGCATT TCAGGTGGGG CCAGAGGTGA GAAAGGACAG
    AAGGGAGAGC CAGCCATCAT TGAACCCGGA ATGCTGATCG AAGGACCACC AGGCCCAGAA
    GGTCCAGCTG GTTATCCCGG TCCTCCTGGT ACCTCAGGTC CTCCTGGTCC AGCTGGAGAT
    CCTGGAGAGA GGGGCCCCCC TGGTCGTGCA GGTCTTCCTG GAGCTGATGG CTTGCCCGGA
    CCACCAGGAA CCATGCTGAT GTTGCCCTTC CGCTTCTCTG GTGGTGGAGA TGGTGGATCT
    AAAGGACCCC AAGTGTCTGC CCAGGAATCA CAAGCCCAAG CTATTCTCCA ACAAGCAAGA
    CTGGCGTTGA GAGGGCCATC TGGTCCGATG GGTCTTACTG GAAGACCAGG CCCATTGGGT
    TCTCCAGGTC CTTCAGGATT AAAGGGAGAG TCAGGAGATT TGGGACCTCA GGGGCCACGT
    GGGCCACAAG GTTCTCCCGG TCCATTAGGA AAACCAGGAC GAAGGGGACG AGCTGGTAGT
    GATGGTGCTC GGGGAATGCC TGGACAAACA GGACCAAAGG GTGACCGAGG CTTTGACGGG
    TTGGGCGGTT TGCCAGGAGA AAAGGGACAT CGTGGTGAGC CTGGTCCACA AGGTCCACCA
    GGGCCACATG GAGAAGACGG AGAAAGGGGT GATGATGGAG ACGTTGGACC TAGAGGGTTA
    CCAGGTGAAC CTGGACCCCG AGGGTTGCTT GGACCTAAAG GACCTCCTGG CCCACCGGGA
    ATCACCGGTG TTCCTGGAAT GGACGGTCAA GTTGGACCCA AAGGTAGTGT GGGACCTCAA
    GGTGAGCCAG GACCACCTGG GCAGCAAGGA AACCCAGGAG CACAGGGTCT TCCAGGTCCT
    CAGGGAGCTA TTGGACCACC AGGAGAGAAG GGACCACTGG GAAAATCAGG TCTTCCCGGA
    ATGCCCGGAG CTGATGGACC TCCCGGTCAC CCCGGGAAAG AAGGACCCTC AGGAGAGAAA
    GGAACCCTGG GTCCTGCAGG GCCACAGGGA CCAATTGGAT ACCCAGGACC ACGTGGTGTT
    AAGGGAGCTG ATGGAATTCG TGGTCTAAAG GGAACAAAAG GAGAAAAGGG TGAAGATGGA
    TTCCCTGGAT TTAAGGGTGA TATGGGCACC AAAGGAGACA GAGGTGAAAT CGGTCCTCCT
    GGTCCTAGAG GTGAAGATGG TCCTGAAGGT CCAAAGGGTC GAGGTGGTCC ATCTGGAGAA
    ATAGGTCCAC TTGGTCCTGG CGGTGAAAAG GGAAAGCTTG GTGTTCCTGG TCTACCAGGT
    TATCCAGGAA GACAAGGTCC AAAGGGTTCT ATCGGATTCC CAGGATTCGC AGGAGCAAAC
    GGAGAGAAAG GAACAAGGGG TACCCCTGGC AAACCGGGTC CAAGGGGGCA ACGAGGTCCA
    ACGGGTCCAC GTGGTGAAAG AGGTCCAAGG GGAGCTACAG GCAAGCCAGG CCCCAAGGGT
    AACTCCGGAG GGGATGGCCC AGCAGGTCCA CCTGGTGAAA GGGGACCACC TGGACCTCAA
    GGACCCACTG GATTTGCAGG ACCGAAGGGC CCCCCTGGTC CACCAGGGAA AGATGGACTT
    CCCGGCCACC CTGGACAGAG AGGAGAGACT GGATTCCAAG GAAAGACTGG CCCTCCTGGT
    CCCCCTGGCG TTGTCGGTCC TCAGGGTCCA ACTGGAGAGA CTGGCCCAAT GGGTGAAAGA
    GGACATCCTG GTCCTCCAGG TCCTCCTGGT GAGCAAGGTT TACCTGGTGT GGCTGGAAAA
    GAAGGATCCA AGGGAGACCC CGGTCCTGCT GGTTTGCCTG GAAAAGATGG ACCTCCAGGT
    CTAAGAGGTT TTACTGGAGA AAGAGGTCTC CCCGGTCCGA TTGGTGTTCC TGGACTGAAA
    GGCAATGAGG GTCCTCCTGG TCCACCTGGC CCAGCTGGTT CCCCCGGTGA AAGAGGAGCT
    GCGGGTCCAG CCGGACCTAT TGGACTGCCT GGAAGACCTG GCCCTCAGGG GCCACCAGGA
    CCTGCCGGCG AGAAGGGTAC ACCAGGTGAG AAAGGTCCCC AGGGACCTGC TGGACGTGAT
    GGTATCCAAG GCCCCGTTGG ACTTCCAGGC CCAGCTGGAC CTGTAGGGCC ACCTGGTGAA
    GATGGTGATA AGGGTGAAAT CGGAGAGCCT GGGCAAAAAG GAAACAGAGG AGATAAAGGA
    GAGCAGGGTC CCCCAGGTCC ACCTGGCCCA CAAGGTCCCA TTGGTCAGCC TGGCCCATCA
    GGATCTGATG GAGAGCCAGG CCCCAGGGGT CAACAAGGTC TTTTTGGACA AAAAGGTGAT
    GAAGGACCTA GAGGTTTTGC TGGACCACCA GGACCTGTTG GACTGCAGGG TTTACCTGGA
    CCACCTGGTG AGAAGGGAGA AACCGGAGAC GTTGGTCAGA TGGGTCCTCC TGGTCCCCCT
    GGTCCTAGAG GTCCTTCTGG CTCCCCTGGA GCTGATGGCC CACAAGGTCC TCCTGGAGGA
    ATTGGCAACC CTGGTCCTGT AGGAGAGAAG GGTGACCCTG GAGAGTCTGG GGAACCTGGT
    CTTCCTGGTG AACTTGGACC ACCGGGCCCT AAAGGTGAAA GAGGTGAAAA GGGAGAAGCG
    GGTCTTCAAG GAACTTCTGG CCCTCCAGGT CCTAAAGGTC CTCCAGGTGA TGATGGTCCA
    AAAGGTAGCC CTGGCCCAGT TGGATTTCCT GGTGATCCTG GTCCTCCTGG AGAATCTGGT
    CCTGCAGGTC AAGATGGTCC ACCTGGTGAT AAGGGAGAGG ACGGTGAATC TGGACAGCCA
    GGGTCACCAG GTCCTACTGG AGAGCCTGGG CCTTCAGGAC CACCTGGAAA GAGGGGTCCT
    CCTGGTCCAA CAGGTCCTGA AGGACGTCAA GGAGAAAAAG GAGCAAAGGG TGAATCAGGT
    TTGGAAGGAC CTCCTGGTAA GACTGGTCCT ATTGGTCCCC AAGGACCTCC CGGAAAACCT
    GGACCTGAAG GCCTTCGAGG AATTCCAGGC CCTGTTGGTG AACAAGGTCT CCCTGGTTCA
    CCTGGACCTG ATGGCCCTCC CGGACCTATG GGTCCACCAG GTATACCAGG TCTGAAAGGA
    GACTCAGGTC CAAAAGGAGA AAAGGGTCAT CCAGGCTTGA TAGGTCTCAT TGGACCTCCT
    GGTGAAACTA AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012824630.1
    CDS344..4918
    Translation

    Target ORF information:

    RefSeq Version XM_012969176.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type V, alpha 1 (col5a1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012969176.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTATGG ATTCCCACAC ACGATGGAAA AGACGGAATT GGACGACAGA GTGGCCCCTC 
    CTTGTAGCTT TAGCCTTGTC TATATGTTCC CGGTTCAGCG CAGCTGAACC GGCGGACCTG
    TTGAAGGCCT TAGAATTTCA CAATTTACCA GACGGTATCA AAAAAACGAC GGGCTTCTGC
    GCCTACCGCA AATCTTCAGA TGGACCAGAC GTGGCATTTA GAGTGTCCAA AGAGGCCCAG
    CTTAGCGCTC CGACCAATCA ATTGTTCTCC GATGTCGCAT TCCCAGAAGA TTTCTCCTTG
    TTGGCAACTG TGAAACCCAA AAAAGGAAGC CATTCCTACC TGCTCTCCAT CTACAATGAG
    CAAGGAACCC TGCAGATCGG CCTGACGCTG GGCTGGGAAC CGATGTTCGT CTATGAAGAC
    CACCAGGGAC ACCCACCCCC CGAGGATTAC CCCATCTTTA GAAACATTAA TCTGTCAGAT
    GGGAAGTGGC ACCGGGTAGC TTTCAGCGTG CACAAGAAGA CGATCACCCT GTTCCTGGAC
    TGCGTCAAGA AGGCCACCCT GCCCTTGAAA AGAAGCGACG GCCCCATTAT AGACACGAAC
    GGCATCGTCG TGTTCGGCAC CAGGATCCTG GACGAGCAAG TCTTTGAGGG AGACATTCAA
    CAGCTGATGA TCGTGCCCGA CGCCCGTGCT GCACATGATT ATTGTGAACA CTACACCCCC
    GACTGTGACA CCGCCATTCC AGATGCACCG CAGTCTCAGG ATCCCAACCA AGATGAATAT
    TACCCAGAAG CTGCTGAAGC CGAAGGCGAG GGTTATTACT ATGAATATCC TTACTACGAA
    GATGAGAAGA CAGAAACTGC AACTACCAAG CCCACGCCTG AGAAAGCCGA GGTACTAAGG
    CCCGAGACAG AAATCGAAGA GGAGGAAACA CCCCCACCAA CTGAAGCCCC TGCTATTCCC
    GTCACTGATG CTCCACGATT TGAGACCAGG GAAGAGAGCA GGGTAGAGAG CAGGGTAGAG
    AGCAGAGTGG ATAACCCCAC CACCGATGAC TACAACTATG AAACCGTACA GGAAGAATAT
    TACACCGCCT CTCCTTATGA AGATGTCAAC TACTCTGAAG GAGAGGAAAC AGGAATTGAG
    ACTGGGACTA CTGGTGAAGG CCTCATCGAG GGAGAAGGAG AAATTTTGAC CAAAACTGTC
    ATCACAAGCA GCTCATCCAA TGGCTCATCG GTCATCAGCA GTGGAAGTTC CAAAAAAGAA
    GGCAGTACCG ACGGAGATTT CACAGAAGTG ACCATACAGG AGTACGACCA AGGGAAAGAT
    ACTTACTATG ACATCTACGA TGATAAGCTT CTCACTGACA CTTCAGACGG AATTGGTGGG
    CAGCCAGCTG AACAAGATCA ATTTGGCATT TCAGGTGGGG CCAGAGGTGA GAAAGGACAG
    AAGGGAGAGC CAGCCATCAT TGAACCCGGA ATGCTGATCG AAGGACCACC AGGCCCAGAA
    GGTCCAGCTG GTTATCCCGG TCCTCCTGGT ACCTCAGGTC CTCCTGGTCC AGCTGGAGAT
    CCTGGAGAGA GGGGCCCCCC TGGTCGTGCA GGTCTTCCTG GAGCTGATGG CTTGCCCGGA
    CCACCAGGAA CCATGCTGAT GTTGCCCTTC CGCTTCTCTG GTGGTGGAGA TGGTGGATCT
    AAAGGACCCC AAGTGTCTGC CCAGGAATCA CAAGCCCAAG CTATTCTCCA ACAAGCAAGA
    CTGGCGTTGA GAGGGCCATC TGGTCCGATG GGTCTTACTG GAAGACCAGG CCCATTGGGT
    TCTCCAGGTC CTTCAGGATT AAAGGGAGAG TCAGGAGATT TGGGACCTCA GGGGCCACGT
    GGGCCACAAG GTTCTCCCGG TCCATTAGGA AAACCAGGAC GAAGGGGACG AGCTGGTAGT
    GATGGTGCTC GGGGAATGCC TGGACAAACA GGACCAAAGG GTGACCGAGG CTTTGACGGG
    TTGGGCGGTT TGCCAGGAGA AAAGGGACAT CGTGGTGAGC CTGGTCCACA AGGTCCACCA
    GGGCCACATG GAGAAGACGG AGAAAGGGGT GATGATGGAG ACGTTGGACC TAGAGGGTTA
    CCAGGTGAAC CTGGACCCCG AGGGTTGCTT GGACCTAAAG GACCTCCTGG CCCACCGGGA
    ATCACCGGTG TTCCTGGAAT GGACGGTCAA GTTGGACCCA AAGGTAGTGT GGGACCTCAA
    GGTGAGCCAG GACCACCTGG GCAGCAAGGA AACCCAGGAG CACAGGGTCT TCCAGGTCCT
    CAGGGAGCTA TTGGACCACC AGGAGAGAAG GGACCACTGG GAAAATCAGG TCTTCCCGGA
    ATGCCCGGAG CTGATGGACC TCCCGGTCAC CCCGGGAAAG AAGGACCCTC AGGAGAGAAA
    GGAACCCTGG GTCCTGCAGG GCCACAGGGA CCAATTGGAT ACCCAGGACC ACGTGGTGTT
    AAGGGAGCTG ATGGAATTCG TGGTCTAAAG GGAACAAAAG GAGAAAAGGG TGAAGATGGA
    TTCCCTGGAT TTAAGGGTGA TATGGGCACC AAAGGAGACA GAGGTGAAAT CGGTCCTCCT
    GGTCCTAGAG GTGAAGATGG TCCTGAAGGT CCAAAGGGTC GAGGTGGTCC ATCTGGAGAA
    ATAGGTCCAC TTGGTCCTGG CGGTGAAAAG GGAAAGCTTG GTGTTCCTGG TCTACCAGGT
    TATCCAGGAA GACAAGGTCC AAAGGGTTCT ATCGGATTCC CAGGATTCGC AGGAGCAAAC
    GGAGAGAAAG GAACAAGGGG TACCCCTGGC AAACCGGGTC CAAGGGGGCA ACGAGGTCCA
    ACGGGTCCAC GTGGTGAAAG AGGTCCAAGG GGAGCTACAG GCAAGCCAGG CCCCAAGGGT
    AACTCCGGAG GGGATGGCCC AGCAGGTCCA CCTGGTGAAA GGGGACCACC TGGACCTCAA
    GGACCCACTG GATTTGCAGG ACCGAAGGGC CCCCCTGGTC CACCAGGGAA AGATGGACTT
    CCCGGCCACC CTGGACAGAG AGGAGAGACT GGATTCCAAG GAAAGACTGG CCCTCCTGGT
    CCCCCTGGCG TTGTCGGTCC TCAGGGTCCA ACTGGAGAGA CTGGCCCAAT GGGTGAAAGA
    GGACATCCTG GTCCTCCAGG TCCTCCTGGT GAGCAAGGTT TACCTGGTGT GGCTGGAAAA
    GAAGGATCCA AGGGAGACCC CGGTCCTGCT GGTTTGCCTG GAAAAGATGG ACCTCCAGGT
    CTAAGAGGTT TTACTGGAGA AAGAGGTCTC CCCGGTCCGA TTGGTGTTCC TGGACTGAAA
    GGCAATGAGG GTCCTCCTGG TCCACCTGGC CCAGCTGGTT CCCCCGGTGA AAGAGGAGCT
    GCGGGTCCAG CCGGACCTAT TGGACTGCCT GGAAGACCTG GCCCTCAGGG GCCACCAGGA
    CCTGCCGGCG AGAAGGGTAC ACCAGGTGAG AAAGGTCCCC AGGGACCTGC TGGACGTGAT
    GGTATCCAAG GCCCCGTTGG ACTTCCAGGC CCAGCTGGAC CTGTAGGGCC ACCTGGTGAA
    GATGGTGATA AGGGTGAAAT CGGAGAGCCT GGGCAAAAAG GAAACAGAGG AGATAAAGGA
    GAGCAGGGTC CCCCAGGTCC ACCTGGCCCA CAAGGTCCCA TTGGTCAGCC TGGCCCATCA
    GGATCTGATG GAGAGCCAGG CCCCAGGGGT CAACAAGGTC TTTTTGGACA AAAAGGTGAT
    GAAGGACCTA GAGGTTTTGC TGGACCACCA GGACCTGTTG GACTGCAGGG TTTACCTGGA
    CCACCTGGTG AGAAGGGAGA AACCGGAGAC GTTGGTCAGA TGGGTCCTCC TGGTCCCCCT
    GGTCCTAGAG GTCCTTCTGG CTCCCCTGGA GCTGATGGCC CACAAGGTCC TCCTGGAGGA
    ATTGGCAACC CTGGTCCTGT AGGAGAGAAG GGTGACCCTG GAGAGTCTGG GGAACCTGGT
    CTTCCTGGTG AACTTGGACC ACCGGGCCCT AAAGGTGAAA GAGGTGAAAA GGGAGAAGCG
    GGTCTTCAAG GAACTTCTGG CCCTCCAGGT CCTAAAGGTC CTCCAGGTGA TGATGGTCCA
    AAAGGTAGCC CTGGCCCAGT TGGATTTCCT GGTGATCCTG GTCCTCCTGG AGAATCTGGT
    CCTGCAGGTC AAGATGGTCC ACCTGGTGAT AAGGGAGAGG ACGGTGAATC TGGACAGCCA
    GGGTCACCAG GTCCTACTGG AGAGCCTGGG CCTTCAGGAC CACCTGGAAA GAGGGGTCCT
    CCTGGTCCAA CAGGTCCTGA AGGACGTCAA GGAGAAAAAG GAGCAAAGGG TGAATCAGGT
    TTGGAAGGAC CTCCTGGTAA GACTGGTCCT ATTGGTCCCC AAGGACCTCC CGGAAAACCT
    GGACCTGAAG GCCTTCGAGG AATTCCAGGC CCTGTTGGTG AACAAGGTCT CCCTGGTTCA
    CCTGGACCTG ATGGCCCTCC CGGACCTATG GGTCCACCAG GTATACCAGG TCTGAAAGGA
    GACTCAGGTC CAAAAGGAGA AAAGGGTCAT CCAGGCTTGA TAGGTCTCAT TGGACCTCCT
    GGTGAAACTA AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa14033
    Clone ID Related Accession (Same CDS sequence) XM_004916645.3 , XM_004916645.4
    Accession Version XM_004916645.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5598bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(V) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683374.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004916645.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGAGTATGG ATTCCCACAC ACGATGGAAA AGACGGAATT GGACGACAGA GTGGCCCCTC 
    CTTGTAGCTT TAGCCTTGTC TATATGTTCC CGGTTCAGCG CAGCTGAACC GGCGGACCTG
    TTGAAGGCCT TAGAATTTCA CAATTTACCA GACGGTATCA AAAAAACGAC GGGCTTCTGC
    GCCTACCGCA AATCTTCAGA TGGACCAGAC GTGGCATTTA GAGTGTCCAA AGAGGCCCAG
    CTTAGCGCTC CGACCAATCA ATTGTTCTCC GATGTCGCAT TCCCAGAAGA TTTCTCCTTG
    TTGGCAACTG TGAAACCCAA AAAAGGAAGC CATTCCTACC TGCTCTCCAT CTACAATGAG
    CAAGGAACCC TGCAGATCGG CCTGACGCTG GGCTGGGAAC CGATGTTCGT CTATGAAGAC
    CACCAGGGAC ACCCACCCCC CGAGGATTAC CCCATCTTTA GAAACATTAA TCTGTCAGAT
    GGGAAGTGGC ACCGGGTAGC TTTCAGCGTG CACAAGAAGA CGATCACCCT GTTCCTGGAC
    TGCGTCAAGA AGGCCACCCT GCCCTTGAAA AGAAGCGACG GCCCCATTAT AGACACGAAC
    GGCATCGTCG TGTTCGGCAC CAGGATCCTG GACGAGCAAG TCTTTGAGGG AGACATTCAA
    CAGCTGATGA TCGTGCCCGA CGCCCGTGCT GCACATGATT ATTGTGAACA CTACACCCCC
    GACTGTGACA CCGCCATTCC AGATGCACCG CAGTCTCAGG ATCCCAACCA AGATGAATAT
    TACCCAGAAG CTGCTGAAGC CGAAGGCGAG GGTTATTACT ATGAATATCC TTACTACGAA
    GATGAGAAGA CAGAAACTGC AACTACCAAG CCCACGCCTG AGAAAGCCGA GGTACTAAGG
    CCCGAGACAG AAATCGAAGA GGAGGAAACA CCCCCACCAA CTGAAGCCCC TGCTATTCCC
    GTCACTGATG CTCCACGATT TGAGACCAGG GAAGAGAGCA GGGTAGAGAG CAGGGTAGAG
    AGCAGAGTGG ATAACCCCAC CACCGATGAC TACAACTATG AAACCGTACA GGAAGAATAT
    TACACCGCCT CTCCTTATGA AGATGTCAAC TACTCTGAAG GAGAGGAAAC AGGAATTGAG
    ACTGGGACTA CTGGTGAAGG CCTCATCGAG GGAGAAGGAG AAATTTTGAC CAAAACTGTC
    ATCACAAGCA GCTCATCCAA TGGCTCATCG GTCATCAGCA GTGGAAGTTC CAAAAAAGAA
    GGCAGTACCG ACGGAGATTT CACAGAAGTG ACCATACAGG AGTACGACCA AGGGAAAGAT
    ACTTACTATG ACATCTACGA TGATAAGCTT CTCACTGACA CTTCAGACGG AATTGGTGGG
    CAGCCAGCTG AACAAGATCA ATTTGGCATT TCAGGTGGGG CCAGAGGTGA GAAAGGACAG
    AAGGGAGAGC CAGCCATCAT TGAACCCGGA ATGCTGATCG AAGGACCACC AGGCCCAGAA
    GGTCCAGCTG GTTATCCCGG TCCTCCTGGT ACCTCAGGTC CTCCTGGTCC AGCTGGAGAT
    CCTGGAGAGA GGGGCCCCCC TGGTCGTGCA GGTCTTCCTG GAGCTGATGG CTTGCCCGGA
    CCACCAGGAA CCATGCTGAT GTTGCCCTTC CGCTTCTCTG GTGGTGGAGA TGGTGGATCT
    AAAGGACCCC AAGTGTCTGC CCAGGAATCA CAAGCCCAAG CTATTCTCCA ACAAGCAAGA
    CTGGCGTTGA GAGGGCCATC TGGTCCGATG GGTCTTACTG GAAGACCAGG CCCATTGGGT
    TCTCCAGGTC CTTCAGGATT AAAGGGAGAG TCAGGAGATT TGGGACCTCA GGGGCCACGT
    GGGCCACAAG GTTCTCCCGG TCCATTAGGA AAACCAGGAC GAAGGGGACG AGCTGGTAGT
    GATGGTGCTC GGGGAATGCC TGGACAAACA GGACCAAAGG GTGACCGAGG CTTTGACGGG
    TTGGGCGGTT TGCCAGGAGA AAAGGGACAT CGTGGTGAGC CTGGTCCACA AGGTCCACCA
    GGGCCACATG GAGAAGACGG AGAAAGGGGT GATGATGGAG ACGTTGGACC TAGAGGGTTA
    CCAGGTGAAC CTGGACCCCG AGGGTTGCTT GGACCTAAAG GACCTCCTGG CCCACCGGGA
    ATCACCGGTG TTCCTGGAAT GGACGGTCAA GTTGGACCCA AAGGTAGTGT GGGACCTCAA
    GGTGAGCCAG GACCACCTGG GCAGCAAGGA AACCCAGGAG CACAGGGTCT TCCAGGTCCT
    CAGGGAGCTA TTGGACCACC AGGAGAGAAG GGACCACTGG GAAAATCAGG TCTTCCCGGA
    ATGCCCGGAG CTGATGGACC TCCCGGTCAC CCCGGGAAAG AAGGACCCTC AGGAGAGAAA
    GGAACCCTGG GTCCTGCAGG GCCACAGGGA CCAATTGGAT ACCCAGGACC ACGTGGTGTT
    AAGGGAGCTG ATGGAATTCG TGGTCTAAAG GGAACAAAAG GAGAAAAGGG TGAAGATGGA
    TTCCCTGGAT TTAAGGGTGA TATGGGCACC AAAGGAGACA GAGGTGAAAT CGGTCCTCCT
    GGTCCTAGAG GTGAAGATGG TCCTGAAGGT CCAAAGGGTC GAGGTGGTCC ATCTGGAGAA
    ATAGGTCCAC TTGGTCCTGG CGGTGAAAAG GGAAAGCTTG GTGTTCCTGG TCTACCAGGT
    TATCCAGGAA GACAAGGTCC AAAGGGTTCT ATCGGATTCC CAGGATTCGC AGGAGCAAAC
    GGAGAGAAAG GAACAAGGGG TACCCCTGGC AAACCGGGTC CAAGGGGGCA ACGAGGTCCA
    ACGGGTCCAC GTGGTGAAAG AGGTCCAAGG GGAGCTACAG GCAAGCCAGG CCCCAAGGGT
    AACTCCGGAG GGGATGGCCC AGCAGGTCCA CCTGGTGAAA GGGGACCACC TGGACCTCAA
    GGACCCACTG GATTTGCAGG ACCGAAGGGC CCCCCTGGTC CACCAGGGAA AGATGGACTT
    CCCGGCCACC CTGGACAGAG AGGAGAGACT GGATTCCAAG GAAAGACTGG CCCTCCTGGT
    CCCCCTGGCG TTGTCGGTCC TCAGGGTCCA ACTGGAGAGA CTGGCCCAAT GGGTGAAAGA
    GGACATCCTG GTCCTCCAGG TCCTCCTGGT GAGCAAGGTT TACCTGGTGT GGCTGGAAAA
    GAAGGATCCA AGGGAGACCC CGGTCCTGCT GGTTTGCCTG GAAAAGATGG ACCTCCAGGT
    CTAAGAGGTT TTACTGGAGA AAGAGGTCTC CCCGGTCCGA TTGGTGTTCC TGGACTGAAA
    GGCAATGAGG GTCCTCCTGG TCCACCTGGC CCAGCTGGTT CCCCCGGTGA AAGAGGAGCT
    GCGGGTCCAG CCGGACCTAT TGGACTGCCT GGAAGACCTG GCCCTCAGGG GCCACCAGGA
    CCTGCCGGCG AGAAGGGTAC ACCAGGTGAG AAAGGTCCCC AGGGACCTGC TGGACGTGAT
    GGTATCCAAG GCCCCGTTGG ACTTCCAGGC CCAGCTGGAC CTGTAGGGCC ACCTGGTGAA
    GATGGTGATA AGGGTGAAAT CGGAGAGCCT GGGCAAAAAG GAAACAGAGG AGATAAAGGA
    GAGCAGGGTC CCCCAGGTCC ACCTGGCCCA CAAGGTCCCA TTGGTCAGCC TGGCCCATCA
    GGATCTGATG GAGAGCCAGG CCCCAGGGGT CAACAAGGTC TTTTTGGACA AAAAGGTGAT
    GAAGGACCTA GAGGTTTTGC TGGACCACCA GGACCTGTTG GACTGCAGGG TTTACCTGGA
    CCACCTGGTG AGAAGGGAGA AACCGGAGAC GTTGGTCAGA TGGGTCCTCC TGGTCCCCCT
    GGTCCTAGAG GTCCTTCTGG CTCCCCTGGA GCTGATGGCC CACAAGGTCC TCCTGGAGGA
    ATTGGCAACC CTGGTCCTGT AGGAGAGAAG GGTGACCCTG GAGAGTCTGG GGAACCTGGT
    CTTCCTGGTG AACTTGGACC ACCGGGCCCT AAAGGTGAAA GAGGTGAAAA GGGAGAAGCG
    GGTCTTCAAG GAACTTCTGG CCCTCCAGGT CCTAAAGGTC CTCCAGGTGA TGATGGTCCA
    AAAGGTAGCC CTGGCCCAGT TGGATTTCCT GGTGATCCTG GTCCTCCTGG AGAATCTGGT
    CCTGCAGGTC AAGATGGTCC ACCTGGTGAT AAGGGAGAGG ACGGTGAATC TGGACAGCCA
    GGGTCACCAG GTCCTACTGG AGAGCCTGGG CCTTCAGGAC CACCTGGAAA GAGGGGTCCT
    CCTGGTCCAA CAGGTCCTGA AGGACGTCAA GGAGAAAAAG GAGCAAAGGG TGAATCAGGT
    TTGGAAGGAC CTCCTGGTAA GACTGGTCCT ATTGGTCCCC AAGGACCTCC CGGAAAACCT
    GGACCTGAAG GCCTTCGAGG AATTCCAGGC CCTGTTGGTG AACAAGGTCT CCCTGGTTCA
    CCTGGACCTG ATGGCCCTCC CGGACCTATG GGTCCACCAG GTATACCAGG TCTGAAAGGA
    GACTCAGGTC CAAAAGGAGA AAAGGGTCAT CCAGGCTTGA TAGGTCTCAT TGGACCTCCT
    GGTGAACAGG GAGAAAAGGG AGACCGTGGT TTGCCAGGCC CCCAGGGACC ACATGGTGCT
    AAAGGAGAAC AGGGTATTCT TGGTCCATCT GGTCCTGTTG GTCCACCTGG TCCTCCAGGC
    TTACCGGGTG GTCCTGGTCC AAAAGGTGCT AAAGGTTCAT CGGGTCCAAC TGGTCCAAAG
    GGTGAATCTG GAATCCCTGG TCCACCAGGT CCTCCAGGAC CTCCAGGCGA GGTGATCCAG
    CCTCTGCCTA TCCATTCCTC AAAGAGGACA AGACGTAGCA TTGATGCAAG TCAAGTAGTG
    GACGATGGAA ATGCAGACAA CTACATGGAC TATGCCGATG GAATGGAGGA AATATTTGGT
    TCTCTCAACT CCCTAAAACA GGAAATTGAA CAAATGAAAC ACCCACTGGG CAATCAGCAG
    AACCCAGCAA GGACTTGCAA GGACCTGCTT CTGTGCCATC CTGAACTCCC AGATGGGGAA
    TATTGGATTG ACCCAAATCA AGGATGCTCT AGAGACTCGT TCAAGGTTTT CTGTAATTTC
    ACAGCCGGTG GAGAATCTTG TATCTTCCCC GACAAGAAGT CTGAAGGAGC TAGAATTACA
    TCTTGGCCCA AGGAAAATCC AGGCTCCTGG TTCAGTGAAT TCAAGCGTGG TAAACTGTTG
    TCCTACGTTG ATTCAAATGG CAACCCCATT GGAGTGGTGC AGATGACCTT CATGAAGCTT
    CTGAGTGCTT CGGTGAAACA GAATATCACC TACAACTGCT ACCAGTCCGT GGCATGGCAC
    GACGCCACCA CCAACAGCTA CGACAAGGCC ATTCGCTTCC TGGGGTCCAA CGACGAAGAA
    ATGTCCTACG ACAACAATCC TTCCATTCGA GCCATTATGG ATGGATGTGC GTCAAAGAAA
    GGTTATCAGA AGACAGTTCT GGAACTCGAC ACGTCCAAAG TGGAACAGGC CCCCATTGTG
    GATATTATGT TCAACGACTT CGGAGATCCA GTACAAAAAT TCGGCTTTGA GGTGGGACCA
    GTGTGTTTCA TGGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004916702.1
    CDS342..5939
    Translation

    Target ORF information:

    RefSeq Version XM_004916645.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type V, alpha 1 (col5a1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004916645.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGAGTATGG ATTCCCACAC ACGATGGAAA AGACGGAATT GGACGACAGA GTGGCCCCTC 
    CTTGTAGCTT TAGCCTTGTC TATATGTTCC CGGTTCAGCG CAGCTGAACC GGCGGACCTG
    TTGAAGGCCT TAGAATTTCA CAATTTACCA GACGGTATCA AAAAAACGAC GGGCTTCTGC
    GCCTACCGCA AATCTTCAGA TGGACCAGAC GTGGCATTTA GAGTGTCCAA AGAGGCCCAG
    CTTAGCGCTC CGACCAATCA ATTGTTCTCC GATGTCGCAT TCCCAGAAGA TTTCTCCTTG
    TTGGCAACTG TGAAACCCAA AAAAGGAAGC CATTCCTACC TGCTCTCCAT CTACAATGAG
    CAAGGAACCC TGCAGATCGG CCTGACGCTG GGCTGGGAAC CGATGTTCGT CTATGAAGAC
    CACCAGGGAC ACCCACCCCC CGAGGATTAC CCCATCTTTA GAAACATTAA TCTGTCAGAT
    GGGAAGTGGC ACCGGGTAGC TTTCAGCGTG CACAAGAAGA CGATCACCCT GTTCCTGGAC
    TGCGTCAAGA AGGCCACCCT GCCCTTGAAA AGAAGCGACG GCCCCATTAT AGACACGAAC
    GGCATCGTCG TGTTCGGCAC CAGGATCCTG GACGAGCAAG TCTTTGAGGG AGACATTCAA
    CAGCTGATGA TCGTGCCCGA CGCCCGTGCT GCACATGATT ATTGTGAACA CTACACCCCC
    GACTGTGACA CCGCCATTCC AGATGCACCG CAGTCTCAGG ATCCCAACCA AGATGAATAT
    TACCCAGAAG CTGCTGAAGC CGAAGGCGAG GGTTATTACT ATGAATATCC TTACTACGAA
    GATGAGAAGA CAGAAACTGC AACTACCAAG CCCACGCCTG AGAAAGCCGA GGTACTAAGG
    CCCGAGACAG AAATCGAAGA GGAGGAAACA CCCCCACCAA CTGAAGCCCC TGCTATTCCC
    GTCACTGATG CTCCACGATT TGAGACCAGG GAAGAGAGCA GGGTAGAGAG CAGGGTAGAG
    AGCAGAGTGG ATAACCCCAC CACCGATGAC TACAACTATG AAACCGTACA GGAAGAATAT
    TACACCGCCT CTCCTTATGA AGATGTCAAC TACTCTGAAG GAGAGGAAAC AGGAATTGAG
    ACTGGGACTA CTGGTGAAGG CCTCATCGAG GGAGAAGGAG AAATTTTGAC CAAAACTGTC
    ATCACAAGCA GCTCATCCAA TGGCTCATCG GTCATCAGCA GTGGAAGTTC CAAAAAAGAA
    GGCAGTACCG ACGGAGATTT CACAGAAGTG ACCATACAGG AGTACGACCA AGGGAAAGAT
    ACTTACTATG ACATCTACGA TGATAAGCTT CTCACTGACA CTTCAGACGG AATTGGTGGG
    CAGCCAGCTG AACAAGATCA ATTTGGCATT TCAGGTGGGG CCAGAGGTGA GAAAGGACAG
    AAGGGAGAGC CAGCCATCAT TGAACCCGGA ATGCTGATCG AAGGACCACC AGGCCCAGAA
    GGTCCAGCTG GTTATCCCGG TCCTCCTGGT ACCTCAGGTC CTCCTGGTCC AGCTGGAGAT
    CCTGGAGAGA GGGGCCCCCC TGGTCGTGCA GGTCTTCCTG GAGCTGATGG CTTGCCCGGA
    CCACCAGGAA CCATGCTGAT GTTGCCCTTC CGCTTCTCTG GTGGTGGAGA TGGTGGATCT
    AAAGGACCCC AAGTGTCTGC CCAGGAATCA CAAGCCCAAG CTATTCTCCA ACAAGCAAGA
    CTGGCGTTGA GAGGGCCATC TGGTCCGATG GGTCTTACTG GAAGACCAGG CCCATTGGGT
    TCTCCAGGTC CTTCAGGATT AAAGGGAGAG TCAGGAGATT TGGGACCTCA GGGGCCACGT
    GGGCCACAAG GTTCTCCCGG TCCATTAGGA AAACCAGGAC GAAGGGGACG AGCTGGTAGT
    GATGGTGCTC GGGGAATGCC TGGACAAACA GGACCAAAGG GTGACCGAGG CTTTGACGGG
    TTGGGCGGTT TGCCAGGAGA AAAGGGACAT CGTGGTGAGC CTGGTCCACA AGGTCCACCA
    GGGCCACATG GAGAAGACGG AGAAAGGGGT GATGATGGAG ACGTTGGACC TAGAGGGTTA
    CCAGGTGAAC CTGGACCCCG AGGGTTGCTT GGACCTAAAG GACCTCCTGG CCCACCGGGA
    ATCACCGGTG TTCCTGGAAT GGACGGTCAA GTTGGACCCA AAGGTAGTGT GGGACCTCAA
    GGTGAGCCAG GACCACCTGG GCAGCAAGGA AACCCAGGAG CACAGGGTCT TCCAGGTCCT
    CAGGGAGCTA TTGGACCACC AGGAGAGAAG GGACCACTGG GAAAATCAGG TCTTCCCGGA
    ATGCCCGGAG CTGATGGACC TCCCGGTCAC CCCGGGAAAG AAGGACCCTC AGGAGAGAAA
    GGAACCCTGG GTCCTGCAGG GCCACAGGGA CCAATTGGAT ACCCAGGACC ACGTGGTGTT
    AAGGGAGCTG ATGGAATTCG TGGTCTAAAG GGAACAAAAG GAGAAAAGGG TGAAGATGGA
    TTCCCTGGAT TTAAGGGTGA TATGGGCACC AAAGGAGACA GAGGTGAAAT CGGTCCTCCT
    GGTCCTAGAG GTGAAGATGG TCCTGAAGGT CCAAAGGGTC GAGGTGGTCC ATCTGGAGAA
    ATAGGTCCAC TTGGTCCTGG CGGTGAAAAG GGAAAGCTTG GTGTTCCTGG TCTACCAGGT
    TATCCAGGAA GACAAGGTCC AAAGGGTTCT ATCGGATTCC CAGGATTCGC AGGAGCAAAC
    GGAGAGAAAG GAACAAGGGG TACCCCTGGC AAACCGGGTC CAAGGGGGCA ACGAGGTCCA
    ACGGGTCCAC GTGGTGAAAG AGGTCCAAGG GGAGCTACAG GCAAGCCAGG CCCCAAGGGT
    AACTCCGGAG GGGATGGCCC AGCAGGTCCA CCTGGTGAAA GGGGACCACC TGGACCTCAA
    GGACCCACTG GATTTGCAGG ACCGAAGGGC CCCCCTGGTC CACCAGGGAA AGATGGACTT
    CCCGGCCACC CTGGACAGAG AGGAGAGACT GGATTCCAAG GAAAGACTGG CCCTCCTGGT
    CCCCCTGGCG TTGTCGGTCC TCAGGGTCCA ACTGGAGAGA CTGGCCCAAT GGGTGAAAGA
    GGACATCCTG GTCCTCCAGG TCCTCCTGGT GAGCAAGGTT TACCTGGTGT GGCTGGAAAA
    GAAGGATCCA AGGGAGACCC CGGTCCTGCT GGTTTGCCTG GAAAAGATGG ACCTCCAGGT
    CTAAGAGGTT TTACTGGAGA AAGAGGTCTC CCCGGTCCGA TTGGTGTTCC TGGACTGAAA
    GGCAATGAGG GTCCTCCTGG TCCACCTGGC CCAGCTGGTT CCCCCGGTGA AAGAGGAGCT
    GCGGGTCCAG CCGGACCTAT TGGACTGCCT GGAAGACCTG GCCCTCAGGG GCCACCAGGA
    CCTGCCGGCG AGAAGGGTAC ACCAGGTGAG AAAGGTCCCC AGGGACCTGC TGGACGTGAT
    GGTATCCAAG GCCCCGTTGG ACTTCCAGGC CCAGCTGGAC CTGTAGGGCC ACCTGGTGAA
    GATGGTGATA AGGGTGAAAT CGGAGAGCCT GGGCAAAAAG GAAACAGAGG AGATAAAGGA
    GAGCAGGGTC CCCCAGGTCC ACCTGGCCCA CAAGGTCCCA TTGGTCAGCC TGGCCCATCA
    GGATCTGATG GAGAGCCAGG CCCCAGGGGT CAACAAGGTC TTTTTGGACA AAAAGGTGAT
    GAAGGACCTA GAGGTTTTGC TGGACCACCA GGACCTGTTG GACTGCAGGG TTTACCTGGA
    CCACCTGGTG AGAAGGGAGA AACCGGAGAC GTTGGTCAGA TGGGTCCTCC TGGTCCCCCT
    GGTCCTAGAG GTCCTTCTGG CTCCCCTGGA GCTGATGGCC CACAAGGTCC TCCTGGAGGA
    ATTGGCAACC CTGGTCCTGT AGGAGAGAAG GGTGACCCTG GAGAGTCTGG GGAACCTGGT
    CTTCCTGGTG AACTTGGACC ACCGGGCCCT AAAGGTGAAA GAGGTGAAAA GGGAGAAGCG
    GGTCTTCAAG GAACTTCTGG CCCTCCAGGT CCTAAAGGTC CTCCAGGTGA TGATGGTCCA
    AAAGGTAGCC CTGGCCCAGT TGGATTTCCT GGTGATCCTG GTCCTCCTGG AGAATCTGGT
    CCTGCAGGTC AAGATGGTCC ACCTGGTGAT AAGGGAGAGG ACGGTGAATC TGGACAGCCA
    GGGTCACCAG GTCCTACTGG AGAGCCTGGG CCTTCAGGAC CACCTGGAAA GAGGGGTCCT
    CCTGGTCCAA CAGGTCCTGA AGGACGTCAA GGAGAAAAAG GAGCAAAGGG TGAATCAGGT
    TTGGAAGGAC CTCCTGGTAA GACTGGTCCT ATTGGTCCCC AAGGACCTCC CGGAAAACCT
    GGACCTGAAG GCCTTCGAGG AATTCCAGGC CCTGTTGGTG AACAAGGTCT CCCTGGTTCA
    CCTGGACCTG ATGGCCCTCC CGGACCTATG GGTCCACCAG GTATACCAGG TCTGAAAGGA
    GACTCAGGTC CAAAAGGAGA AAAGGGTCAT CCAGGCTTGA TAGGTCTCAT TGGACCTCCT
    GGTGAACAGG GAGAAAAGGG AGACCGTGGT TTGCCAGGCC CCCAGGGACC ACATGGTGCT
    AAAGGAGAAC AGGGTATTCT TGGTCCATCT GGTCCTGTTG GTCCACCTGG TCCTCCAGGC
    TTACCGGGTG GTCCTGGTCC AAAAGGTGCT AAAGGTTCAT CGGGTCCAAC TGGTCCAAAG
    GGTGAATCTG GAATCCCTGG TCCACCAGGT CCTCCAGGAC CTCCAGGCGA GGTGATCCAG
    CCTCTGCCTA TCCATTCCTC AAAGAGGACA AGACGTAGCA TTGATGCAAG TCAAGTAGTG
    GACGATGGAA ATGCAGACAA CTACATGGAC TATGCCGATG GAATGGAGGA AATATTTGGT
    TCTCTCAACT CCCTAAAACA GGAAATTGAA CAAATGAAAC ACCCACTGGG CAATCAGCAG
    AACCCAGCAA GGACTTGCAA GGACCTGCTT CTGTGCCATC CTGAACTCCC AGATGGGGAA
    TATTGGATTG ACCCAAATCA AGGATGCTCT AGAGACTCGT TCAAGGTTTT CTGTAATTTC
    ACAGCCGGTG GAGAATCTTG TATCTTCCCC GACAAGAAGT CTGAAGGAGC TAGAATTACA
    TCTTGGCCCA AGGAAAATCC AGGCTCCTGG TTCAGTGAAT TCAAGCGTGG TAAACTGTTG
    TCCTACGTTG ATTCAAATGG CAACCCCATT GGAGTGGTGC AGATGACCTT CATGAAGCTT
    CTGAGTGCTT CGGTGAAACA GAATATCACC TACAACTGCT ACCAGTCCGT GGCATGGCAC
    GACGCCACCA CCAACAGCTA CGACAAGGCC ATTCGCTTCC TGGGGTCCAA CGACGAAGAA
    ATGTCCTACG ACAACAATCC TTCCATTCGA GCCATTATGG ATGGATGTGC GTCAAAGAAA
    GGTTATCAGA AGACAGTTCT GGAACTCGAC ACGTCCAAAG TGGAACAGGC CCCCATTGTG
    GATATTATGT TCAACGACTT CGGAGATCCA GTACAAAAAT TCGGCTTTGA GGTGGGACCA
    GTGTGTTTCA TGGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30187
    Clone ID Related Accession (Same CDS sequence) XM_012969175.2 , XM_012969175.3
    Accession Version XM_012969175.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5598bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(V) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683374.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012969175.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGAGTATGG ATTCCCACAC ACGATGGAAA AGACGGAATT GGACGACAGA GTGGCCCCTC 
    CTTGTAGCTT TAGCCTTGTC TATATGTTCC CGGTTCAGCG CAGCTGAACC GGCGGACCTG
    TTGAAGGCCT TAGAATTTCA CAATTTACCA GACGGTATCA AAAAAACGAC GGGCTTCTGC
    GCCTACCGCA AATCTTCAGA TGGACCAGAC GTGGCATTTA GAGTGTCCAA AGAGGCCCAG
    CTTAGCGCTC CGACCAATCA ATTGTTCTCC GATGTCGCAT TCCCAGAAGA TTTCTCCTTG
    TTGGCAACTG TGAAACCCAA AAAAGGAAGC CATTCCTACC TGCTCTCCAT CTACAATGAG
    CAAGGAACCC TGCAGATCGG CCTGACGCTG GGCTGGGAAC CGATGTTCGT CTATGAAGAC
    CACCAGGGAC ACCCACCCCC CGAGGATTAC CCCATCTTTA GAAACATTAA TCTGTCAGAT
    GGGAAGTGGC ACCGGGTAGC TTTCAGCGTG CACAAGAAGA CGATCACCCT GTTCCTGGAC
    TGCGTCAAGA AGGCCACCCT GCCCTTGAAA AGAAGCGACG GCCCCATTAT AGACACGAAC
    GGCATCGTCG TGTTCGGCAC CAGGATCCTG GACGAGCAAG TCTTTGAGGG AGACATTCAA
    CAGCTGATGA TCGTGCCCGA CGCCCGTGCT GCACATGATT ATTGTGAACA CTACACCCCC
    GACTGTGACA CCGCCATTCC AGATGCACCG CAGTCTCAGG ATCCCAACCA AGATGAATAT
    TACCCAGAAG CTGCTGAAGC CGAAGGCGAG GGTTATTACT ATGAATATCC TTACTACGAA
    GATGAGAAGA CAGAAACTGC AACTACCAAG CCCACGCCTG AGAAAGCCGA GGTACTAAGG
    CCCGAGACAG AAATCGAAGA GGAGGAAACA CCCCCACCAA CTGAAGCCCC TGCTATTCCC
    GTCACTGATG CTCCACGATT TGAGACCAGG GAAGAGAGCA GGGTAGAGAG CAGGGTAGAG
    AGCAGAGTGG ATAACCCCAC CACCGATGAC TACAACTATG AAACCGTACA GGAAGAATAT
    TACACCGCCT CTCCTTATGA AGATGTCAAC TACTCTGAAG GAGAGGAAAC AGGAATTGAG
    ACTGGGACTA CTGGTGAAGG CCTCATCGAG GGAGAAGGAG AAATTTTGAC CAAAACTGTC
    ATCACAAGCA GCTCATCCAA TGGCTCATCG GTCATCAGCA GTGGAAGTTC CAAAAAAGAA
    GGCAGTACCG ACGGAGATTT CACAGAAGTG ACCATACAGG AGTACGACCA AGGGAAAGAT
    ACTTACTATG ACATCTACGA TGATAAGCTT CTCACTGACA CTTCAGACGG AATTGGTGGG
    CAGCCAGCTG AACAAGATCA ATTTGGCATT TCAGGTGGGG CCAGAGGTGA GAAAGGACAG
    AAGGGAGAGC CAGCCATCAT TGAACCCGGA ATGCTGATCG AAGGACCACC AGGCCCAGAA
    GGTCCAGCTG GTTATCCCGG TCCTCCTGGT ACCTCAGGTC CTCCTGGTCC AGCTGGAGAT
    CCTGGAGAGA GGGGCCCCCC TGGTCGTGCA GGTCTTCCTG GAGCTGATGG CTTGCCCGGA
    CCACCAGGAA CCATGCTGAT GTTGCCCTTC CGCTTCTCTG GTGGTGGAGA TGGTGGATCT
    AAAGGACCCC AAGTGTCTGC CCAGGAATCA CAAGCCCAAG CTATTCTCCA ACAAGCAAGA
    CTGGCGTTGA GAGGGCCATC TGGTCCGATG GGTCTTACTG GAAGACCAGG CCCATTGGGT
    TCTCCAGGTC CTTCAGGATT AAAGGGAGAG TCAGGAGATT TGGGACCTCA GGGGCCACGT
    GGGCCACAAG GTTCTCCCGG TCCATTAGGA AAACCAGGAC GAAGGGGACG AGCTGGTAGT
    GATGGTGCTC GGGGAATGCC TGGACAAACA GGACCAAAGG GTGACCGAGG CTTTGACGGG
    TTGGGCGGTT TGCCAGGAGA AAAGGGACAT CGTGGTGAGC CTGGTCCACA AGGTCCACCA
    GGGCCACATG GAGAAGACGG AGAAAGGGGT GATGATGGAG ACGTTGGACC TAGAGGGTTA
    CCAGGTGAAC CTGGACCCCG AGGGTTGCTT GGACCTAAAG GACCTCCTGG CCCACCGGGA
    ATCACCGGTG TTCCTGGAAT GGACGGTCAA GTTGGACCCA AAGGTAGTGT GGGACCTCAA
    GGTGAGCCAG GACCACCTGG GCAGCAAGGA AACCCAGGAG CACAGGGTCT TCCAGGTCCT
    CAGGGAGCTA TTGGACCACC AGGAGAGAAG GGACCACTGG GAAAATCAGG TCTTCCCGGA
    ATGCCCGGAG CTGATGGACC TCCCGGTCAC CCCGGGAAAG AAGGACCCTC AGGAGAGAAA
    GGAACCCTGG GTCCTGCAGG GCCACAGGGA CCAATTGGAT ACCCAGGACC ACGTGGTGTT
    AAGGGAGCTG ATGGAATTCG TGGTCTAAAG GGAACAAAAG GAGAAAAGGG TGAAGATGGA
    TTCCCTGGAT TTAAGGGTGA TATGGGCACC AAAGGAGACA GAGGTGAAAT CGGTCCTCCT
    GGTCCTAGAG GTGAAGATGG TCCTGAAGGT CCAAAGGGTC GAGGTGGTCC ATCTGGAGAA
    ATAGGTCCAC TTGGTCCTGG CGGTGAAAAG GGAAAGCTTG GTGTTCCTGG TCTACCAGGT
    TATCCAGGAA GACAAGGTCC AAAGGGTTCT ATCGGATTCC CAGGATTCGC AGGAGCAAAC
    GGAGAGAAAG GAACAAGGGG TACCCCTGGC AAACCGGGTC CAAGGGGGCA ACGAGGTCCA
    ACGGGTCCAC GTGGTGAAAG AGGTCCAAGG GGAGCTACAG GCAAGCCAGG CCCCAAGGGT
    AACTCCGGAG GGGATGGCCC AGCAGGTCCA CCTGGTGAAA GGGGACCACC TGGACCTCAA
    GGACCCACTG GATTTGCAGG ACCGAAGGGC CCCCCTGGTC CACCAGGGAA AGATGGACTT
    CCCGGCCACC CTGGACAGAG AGGAGAGACT GGATTCCAAG GAAAGACTGG CCCTCCTGGT
    CCCCCTGGCG TTGTCGGTCC TCAGGGTCCA ACTGGAGAGA CTGGCCCAAT GGGTGAAAGA
    GGACATCCTG GTCCTCCAGG TCCTCCTGGT GAGCAAGGTT TACCTGGTGT GGCTGGAAAA
    GAAGGATCCA AGGGAGACCC CGGTCCTGCT GGTTTGCCTG GAAAAGATGG ACCTCCAGGT
    CTAAGAGGTT TTACTGGAGA AAGAGGTCTC CCCGGTCCGA TTGGTGTTCC TGGACTGAAA
    GGCAATGAGG GTCCTCCTGG TCCACCTGGC CCAGCTGGTT CCCCCGGTGA AAGAGGAGCT
    GCGGGTCCAG CCGGACCTAT TGGACTGCCT GGAAGACCTG GCCCTCAGGG GCCACCAGGA
    CCTGCCGGCG AGAAGGGTAC ACCAGGTGAG AAAGGTCCCC AGGGACCTGC TGGACGTGAT
    GGTATCCAAG GCCCCGTTGG ACTTCCAGGC CCAGCTGGAC CTGTAGGGCC ACCTGGTGAA
    GATGGTGATA AGGGTGAAAT CGGAGAGCCT GGGCAAAAAG GAAACAGAGG AGATAAAGGA
    GAGCAGGGTC CCCCAGGTCC ACCTGGCCCA CAAGGTCCCA TTGGTCAGCC TGGCCCATCA
    GGATCTGATG GAGAGCCAGG CCCCAGGGGT CAACAAGGTC TTTTTGGACA AAAAGGTGAT
    GAAGGACCTA GAGGTTTTGC TGGACCACCA GGACCTGTTG GACTGCAGGG TTTACCTGGA
    CCACCTGGTG AGAAGGGAGA AACCGGAGAC GTTGGTCAGA TGGGTCCTCC TGGTCCCCCT
    GGTCCTAGAG GTCCTTCTGG CTCCCCTGGA GCTGATGGCC CACAAGGTCC TCCTGGAGGA
    ATTGGCAACC CTGGTCCTGT AGGAGAGAAG GGTGACCCTG GAGAGTCTGG GGAACCTGGT
    CTTCCTGGTG AACTTGGACC ACCGGGCCCT AAAGGTGAAA GAGGTGAAAA GGGAGAAGCG
    GGTCTTCAAG GAACTTCTGG CCCTCCAGGT CCTAAAGGTC CTCCAGGTGA TGATGGTCCA
    AAAGGTAGCC CTGGCCCAGT TGGATTTCCT GGTGATCCTG GTCCTCCTGG AGAATCTGGT
    CCTGCAGGTC AAGATGGTCC ACCTGGTGAT AAGGGAGAGG ACGGTGAATC TGGACAGCCA
    GGGTCACCAG GTCCTACTGG AGAGCCTGGG CCTTCAGGAC CACCTGGAAA GAGGGGTCCT
    CCTGGTCCAA CAGGTCCTGA AGGACGTCAA GGAGAAAAAG GAGCAAAGGG TGAATCAGGT
    TTGGAAGGAC CTCCTGGTAA GACTGGTCCT ATTGGTCCCC AAGGACCTCC CGGAAAACCT
    GGACCTGAAG GCCTTCGAGG AATTCCAGGC CCTGTTGGTG AACAAGGTCT CCCTGGTTCA
    CCTGGACCTG ATGGCCCTCC CGGACCTATG GGTCCACCAG GTATACCAGG TCTGAAAGGA
    GACTCAGGTC CAAAAGGAGA AAAGGGTCAT CCAGGCTTGA TAGGTCTCAT TGGACCTCCT
    GGTGAACAGG GAGAAAAGGG AGACCGTGGT TTGCCAGGCC CCCAGGGACC ACATGGTGCT
    AAAGGAGAAC AGGGTATTCT TGGTCCATCT GGTCCTGTTG GTCCACCTGG TCCTCCAGGC
    TTACCGGGTG GTCCTGGTCC AAAAGGTGCT AAAGGTTCAT CGGGTCCAAC TGGTCCAAAG
    GGTGAATCTG GAATCCCTGG TCCACCAGGT CCTCCAGGAC CTCCAGGCGA GGTGATCCAG
    CCTCTGCCTA TCCATTCCTC AAAGAGGACA AGACGTAGCA TTGATGCAAG TCAAGTAGTG
    GACGATGGAA ATGCAGACAA CTACATGGAC TATGCCGATG GAATGGAGGA AATATTTGGT
    TCTCTCAACT CCCTAAAACA GGAAATTGAA CAAATGAAAC ACCCACTGGG CAATCAGCAG
    AACCCAGCAA GGACTTGCAA GGACCTGCTT CTGTGCCATC CTGAACTCCC AGATGGGGAA
    TATTGGATTG ACCCAAATCA AGGATGCTCT AGAGACTCGT TCAAGGTTTT CTGTAATTTC
    ACAGCCGGTG GAGAATCTTG TATCTTCCCC GACAAGAAGT CTGAAGGAAA TAAACTGGCT
    CGTTGGCCCA AAGAGCTGCC TGGTACATGG TATAGTCATT ACAAGCGGGG TTCCATGTTG
    TCCTACGTTG ATTCAAATGG CAACCCCATT GGAGTGGTGC AGATGACCTT CATGAAGCTT
    CTGAGTGCTT CGGTGAAACA GAATATCACC TACAACTGCT ACCAGTCCGT GGCATGGCAC
    GACGCCACCA CCAACAGCTA CGACAAGGCC ATTCGCTTCC TGGGGTCCAA CGACGAAGAA
    ATGTCCTACG ACAACAATCC TTCCATTCGA GCCATTATGG ATGGATGTGC GTCAAAGAAA
    GGTTATCAGA AGACAGTTCT GGAACTCGAC ACGTCCAAAG TGGAACAGGC CCCCATTGTG
    GATATTATGT TCAACGACTT CGGAGATCCA GTACAAAAAT TCGGCTTTGA GGTGGGACCA
    GTGTGTTTCA TGGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012824629.1
    CDS342..5939
    Translation

    Target ORF information:

    RefSeq Version XM_012969175.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type V, alpha 1 (col5a1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012969175.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGAGTATGG ATTCCCACAC ACGATGGAAA AGACGGAATT GGACGACAGA GTGGCCCCTC 
    CTTGTAGCTT TAGCCTTGTC TATATGTTCC CGGTTCAGCG CAGCTGAACC GGCGGACCTG
    TTGAAGGCCT TAGAATTTCA CAATTTACCA GACGGTATCA AAAAAACGAC GGGCTTCTGC
    GCCTACCGCA AATCTTCAGA TGGACCAGAC GTGGCATTTA GAGTGTCCAA AGAGGCCCAG
    CTTAGCGCTC CGACCAATCA ATTGTTCTCC GATGTCGCAT TCCCAGAAGA TTTCTCCTTG
    TTGGCAACTG TGAAACCCAA AAAAGGAAGC CATTCCTACC TGCTCTCCAT CTACAATGAG
    CAAGGAACCC TGCAGATCGG CCTGACGCTG GGCTGGGAAC CGATGTTCGT CTATGAAGAC
    CACCAGGGAC ACCCACCCCC CGAGGATTAC CCCATCTTTA GAAACATTAA TCTGTCAGAT
    GGGAAGTGGC ACCGGGTAGC TTTCAGCGTG CACAAGAAGA CGATCACCCT GTTCCTGGAC
    TGCGTCAAGA AGGCCACCCT GCCCTTGAAA AGAAGCGACG GCCCCATTAT AGACACGAAC
    GGCATCGTCG TGTTCGGCAC CAGGATCCTG GACGAGCAAG TCTTTGAGGG AGACATTCAA
    CAGCTGATGA TCGTGCCCGA CGCCCGTGCT GCACATGATT ATTGTGAACA CTACACCCCC
    GACTGTGACA CCGCCATTCC AGATGCACCG CAGTCTCAGG ATCCCAACCA AGATGAATAT
    TACCCAGAAG CTGCTGAAGC CGAAGGCGAG GGTTATTACT ATGAATATCC TTACTACGAA
    GATGAGAAGA CAGAAACTGC AACTACCAAG CCCACGCCTG AGAAAGCCGA GGTACTAAGG
    CCCGAGACAG AAATCGAAGA GGAGGAAACA CCCCCACCAA CTGAAGCCCC TGCTATTCCC
    GTCACTGATG CTCCACGATT TGAGACCAGG GAAGAGAGCA GGGTAGAGAG CAGGGTAGAG
    AGCAGAGTGG ATAACCCCAC CACCGATGAC TACAACTATG AAACCGTACA GGAAGAATAT
    TACACCGCCT CTCCTTATGA AGATGTCAAC TACTCTGAAG GAGAGGAAAC AGGAATTGAG
    ACTGGGACTA CTGGTGAAGG CCTCATCGAG GGAGAAGGAG AAATTTTGAC CAAAACTGTC
    ATCACAAGCA GCTCATCCAA TGGCTCATCG GTCATCAGCA GTGGAAGTTC CAAAAAAGAA
    GGCAGTACCG ACGGAGATTT CACAGAAGTG ACCATACAGG AGTACGACCA AGGGAAAGAT
    ACTTACTATG ACATCTACGA TGATAAGCTT CTCACTGACA CTTCAGACGG AATTGGTGGG
    CAGCCAGCTG AACAAGATCA ATTTGGCATT TCAGGTGGGG CCAGAGGTGA GAAAGGACAG
    AAGGGAGAGC CAGCCATCAT TGAACCCGGA ATGCTGATCG AAGGACCACC AGGCCCAGAA
    GGTCCAGCTG GTTATCCCGG TCCTCCTGGT ACCTCAGGTC CTCCTGGTCC AGCTGGAGAT
    CCTGGAGAGA GGGGCCCCCC TGGTCGTGCA GGTCTTCCTG GAGCTGATGG CTTGCCCGGA
    CCACCAGGAA CCATGCTGAT GTTGCCCTTC CGCTTCTCTG GTGGTGGAGA TGGTGGATCT
    AAAGGACCCC AAGTGTCTGC CCAGGAATCA CAAGCCCAAG CTATTCTCCA ACAAGCAAGA
    CTGGCGTTGA GAGGGCCATC TGGTCCGATG GGTCTTACTG GAAGACCAGG CCCATTGGGT
    TCTCCAGGTC CTTCAGGATT AAAGGGAGAG TCAGGAGATT TGGGACCTCA GGGGCCACGT
    GGGCCACAAG GTTCTCCCGG TCCATTAGGA AAACCAGGAC GAAGGGGACG AGCTGGTAGT
    GATGGTGCTC GGGGAATGCC TGGACAAACA GGACCAAAGG GTGACCGAGG CTTTGACGGG
    TTGGGCGGTT TGCCAGGAGA AAAGGGACAT CGTGGTGAGC CTGGTCCACA AGGTCCACCA
    GGGCCACATG GAGAAGACGG AGAAAGGGGT GATGATGGAG ACGTTGGACC TAGAGGGTTA
    CCAGGTGAAC CTGGACCCCG AGGGTTGCTT GGACCTAAAG GACCTCCTGG CCCACCGGGA
    ATCACCGGTG TTCCTGGAAT GGACGGTCAA GTTGGACCCA AAGGTAGTGT GGGACCTCAA
    GGTGAGCCAG GACCACCTGG GCAGCAAGGA AACCCAGGAG CACAGGGTCT TCCAGGTCCT
    CAGGGAGCTA TTGGACCACC AGGAGAGAAG GGACCACTGG GAAAATCAGG TCTTCCCGGA
    ATGCCCGGAG CTGATGGACC TCCCGGTCAC CCCGGGAAAG AAGGACCCTC AGGAGAGAAA
    GGAACCCTGG GTCCTGCAGG GCCACAGGGA CCAATTGGAT ACCCAGGACC ACGTGGTGTT
    AAGGGAGCTG ATGGAATTCG TGGTCTAAAG GGAACAAAAG GAGAAAAGGG TGAAGATGGA
    TTCCCTGGAT TTAAGGGTGA TATGGGCACC AAAGGAGACA GAGGTGAAAT CGGTCCTCCT
    GGTCCTAGAG GTGAAGATGG TCCTGAAGGT CCAAAGGGTC GAGGTGGTCC ATCTGGAGAA
    ATAGGTCCAC TTGGTCCTGG CGGTGAAAAG GGAAAGCTTG GTGTTCCTGG TCTACCAGGT
    TATCCAGGAA GACAAGGTCC AAAGGGTTCT ATCGGATTCC CAGGATTCGC AGGAGCAAAC
    GGAGAGAAAG GAACAAGGGG TACCCCTGGC AAACCGGGTC CAAGGGGGCA ACGAGGTCCA
    ACGGGTCCAC GTGGTGAAAG AGGTCCAAGG GGAGCTACAG GCAAGCCAGG CCCCAAGGGT
    AACTCCGGAG GGGATGGCCC AGCAGGTCCA CCTGGTGAAA GGGGACCACC TGGACCTCAA
    GGACCCACTG GATTTGCAGG ACCGAAGGGC CCCCCTGGTC CACCAGGGAA AGATGGACTT
    CCCGGCCACC CTGGACAGAG AGGAGAGACT GGATTCCAAG GAAAGACTGG CCCTCCTGGT
    CCCCCTGGCG TTGTCGGTCC TCAGGGTCCA ACTGGAGAGA CTGGCCCAAT GGGTGAAAGA
    GGACATCCTG GTCCTCCAGG TCCTCCTGGT GAGCAAGGTT TACCTGGTGT GGCTGGAAAA
    GAAGGATCCA AGGGAGACCC CGGTCCTGCT GGTTTGCCTG GAAAAGATGG ACCTCCAGGT
    CTAAGAGGTT TTACTGGAGA AAGAGGTCTC CCCGGTCCGA TTGGTGTTCC TGGACTGAAA
    GGCAATGAGG GTCCTCCTGG TCCACCTGGC CCAGCTGGTT CCCCCGGTGA AAGAGGAGCT
    GCGGGTCCAG CCGGACCTAT TGGACTGCCT GGAAGACCTG GCCCTCAGGG GCCACCAGGA
    CCTGCCGGCG AGAAGGGTAC ACCAGGTGAG AAAGGTCCCC AGGGACCTGC TGGACGTGAT
    GGTATCCAAG GCCCCGTTGG ACTTCCAGGC CCAGCTGGAC CTGTAGGGCC ACCTGGTGAA
    GATGGTGATA AGGGTGAAAT CGGAGAGCCT GGGCAAAAAG GAAACAGAGG AGATAAAGGA
    GAGCAGGGTC CCCCAGGTCC ACCTGGCCCA CAAGGTCCCA TTGGTCAGCC TGGCCCATCA
    GGATCTGATG GAGAGCCAGG CCCCAGGGGT CAACAAGGTC TTTTTGGACA AAAAGGTGAT
    GAAGGACCTA GAGGTTTTGC TGGACCACCA GGACCTGTTG GACTGCAGGG TTTACCTGGA
    CCACCTGGTG AGAAGGGAGA AACCGGAGAC GTTGGTCAGA TGGGTCCTCC TGGTCCCCCT
    GGTCCTAGAG GTCCTTCTGG CTCCCCTGGA GCTGATGGCC CACAAGGTCC TCCTGGAGGA
    ATTGGCAACC CTGGTCCTGT AGGAGAGAAG GGTGACCCTG GAGAGTCTGG GGAACCTGGT
    CTTCCTGGTG AACTTGGACC ACCGGGCCCT AAAGGTGAAA GAGGTGAAAA GGGAGAAGCG
    GGTCTTCAAG GAACTTCTGG CCCTCCAGGT CCTAAAGGTC CTCCAGGTGA TGATGGTCCA
    AAAGGTAGCC CTGGCCCAGT TGGATTTCCT GGTGATCCTG GTCCTCCTGG AGAATCTGGT
    CCTGCAGGTC AAGATGGTCC ACCTGGTGAT AAGGGAGAGG ACGGTGAATC TGGACAGCCA
    GGGTCACCAG GTCCTACTGG AGAGCCTGGG CCTTCAGGAC CACCTGGAAA GAGGGGTCCT
    CCTGGTCCAA CAGGTCCTGA AGGACGTCAA GGAGAAAAAG GAGCAAAGGG TGAATCAGGT
    TTGGAAGGAC CTCCTGGTAA GACTGGTCCT ATTGGTCCCC AAGGACCTCC CGGAAAACCT
    GGACCTGAAG GCCTTCGAGG AATTCCAGGC CCTGTTGGTG AACAAGGTCT CCCTGGTTCA
    CCTGGACCTG ATGGCCCTCC CGGACCTATG GGTCCACCAG GTATACCAGG TCTGAAAGGA
    GACTCAGGTC CAAAAGGAGA AAAGGGTCAT CCAGGCTTGA TAGGTCTCAT TGGACCTCCT
    GGTGAACAGG GAGAAAAGGG AGACCGTGGT TTGCCAGGCC CCCAGGGACC ACATGGTGCT
    AAAGGAGAAC AGGGTATTCT TGGTCCATCT GGTCCTGTTG GTCCACCTGG TCCTCCAGGC
    TTACCGGGTG GTCCTGGTCC AAAAGGTGCT AAAGGTTCAT CGGGTCCAAC TGGTCCAAAG
    GGTGAATCTG GAATCCCTGG TCCACCAGGT CCTCCAGGAC CTCCAGGCGA GGTGATCCAG
    CCTCTGCCTA TCCATTCCTC AAAGAGGACA AGACGTAGCA TTGATGCAAG TCAAGTAGTG
    GACGATGGAA ATGCAGACAA CTACATGGAC TATGCCGATG GAATGGAGGA AATATTTGGT
    TCTCTCAACT CCCTAAAACA GGAAATTGAA CAAATGAAAC ACCCACTGGG CAATCAGCAG
    AACCCAGCAA GGACTTGCAA GGACCTGCTT CTGTGCCATC CTGAACTCCC AGATGGGGAA
    TATTGGATTG ACCCAAATCA AGGATGCTCT AGAGACTCGT TCAAGGTTTT CTGTAATTTC
    ACAGCCGGTG GAGAATCTTG TATCTTCCCC GACAAGAAGT CTGAAGGAAA TAAACTGGCT
    CGTTGGCCCA AAGAGCTGCC TGGTACATGG TATAGTCATT ACAAGCGGGG TTCCATGTTG
    TCCTACGTTG ATTCAAATGG CAACCCCATT GGAGTGGTGC AGATGACCTT CATGAAGCTT
    CTGAGTGCTT CGGTGAAACA GAATATCACC TACAACTGCT ACCAGTCCGT GGCATGGCAC
    GACGCCACCA CCAACAGCTA CGACAAGGCC ATTCGCTTCC TGGGGTCCAA CGACGAAGAA
    ATGTCCTACG ACAACAATCC TTCCATTCGA GCCATTATGG ATGGATGTGC GTCAAAGAAA
    GGTTATCAGA AGACAGTTCT GGAACTCGAC ACGTCCAAAG TGGAACAGGC CCCCATTGTG
    GATATTATGT TCAACGACTT CGGAGATCCA GTACAAAAAT TCGGCTTTGA GGTGGGACCA
    GTGTGTTTCA TGGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30188
    Clone ID Related Accession (Same CDS sequence) XM_012969176.2 , XM_012969176.3
    Accession Version XM_012969176.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4575bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(V) chain isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683374.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012969176.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTATGG ATTCCCACAC ACGATGGAAA AGACGGAATT GGACGACAGA GTGGCCCCTC 
    CTTGTAGCTT TAGCCTTGTC TATATGTTCC CGGTTCAGCG CAGCTGAACC GGCGGACCTG
    TTGAAGGCCT TAGAATTTCA CAATTTACCA GACGGTATCA AAAAAACGAC GGGCTTCTGC
    GCCTACCGCA AATCTTCAGA TGGACCAGAC GTGGCATTTA GAGTGTCCAA AGAGGCCCAG
    CTTAGCGCTC CGACCAATCA ATTGTTCTCC GATGTCGCAT TCCCAGAAGA TTTCTCCTTG
    TTGGCAACTG TGAAACCCAA AAAAGGAAGC CATTCCTACC TGCTCTCCAT CTACAATGAG
    CAAGGAACCC TGCAGATCGG CCTGACGCTG GGCTGGGAAC CGATGTTCGT CTATGAAGAC
    CACCAGGGAC ACCCACCCCC CGAGGATTAC CCCATCTTTA GAAACATTAA TCTGTCAGAT
    GGGAAGTGGC ACCGGGTAGC TTTCAGCGTG CACAAGAAGA CGATCACCCT GTTCCTGGAC
    TGCGTCAAGA AGGCCACCCT GCCCTTGAAA AGAAGCGACG GCCCCATTAT AGACACGAAC
    GGCATCGTCG TGTTCGGCAC CAGGATCCTG GACGAGCAAG TCTTTGAGGG AGACATTCAA
    CAGCTGATGA TCGTGCCCGA CGCCCGTGCT GCACATGATT ATTGTGAACA CTACACCCCC
    GACTGTGACA CCGCCATTCC AGATGCACCG CAGTCTCAGG ATCCCAACCA AGATGAATAT
    TACCCAGAAG CTGCTGAAGC CGAAGGCGAG GGTTATTACT ATGAATATCC TTACTACGAA
    GATGAGAAGA CAGAAACTGC AACTACCAAG CCCACGCCTG AGAAAGCCGA GGTACTAAGG
    CCCGAGACAG AAATCGAAGA GGAGGAAACA CCCCCACCAA CTGAAGCCCC TGCTATTCCC
    GTCACTGATG CTCCACGATT TGAGACCAGG GAAGAGAGCA GGGTAGAGAG CAGGGTAGAG
    AGCAGAGTGG ATAACCCCAC CACCGATGAC TACAACTATG AAACCGTACA GGAAGAATAT
    TACACCGCCT CTCCTTATGA AGATGTCAAC TACTCTGAAG GAGAGGAAAC AGGAATTGAG
    ACTGGGACTA CTGGTGAAGG CCTCATCGAG GGAGAAGGAG AAATTTTGAC CAAAACTGTC
    ATCACAAGCA GCTCATCCAA TGGCTCATCG GTCATCAGCA GTGGAAGTTC CAAAAAAGAA
    GGCAGTACCG ACGGAGATTT CACAGAAGTG ACCATACAGG AGTACGACCA AGGGAAAGAT
    ACTTACTATG ACATCTACGA TGATAAGCTT CTCACTGACA CTTCAGACGG AATTGGTGGG
    CAGCCAGCTG AACAAGATCA ATTTGGCATT TCAGGTGGGG CCAGAGGTGA GAAAGGACAG
    AAGGGAGAGC CAGCCATCAT TGAACCCGGA ATGCTGATCG AAGGACCACC AGGCCCAGAA
    GGTCCAGCTG GTTATCCCGG TCCTCCTGGT ACCTCAGGTC CTCCTGGTCC AGCTGGAGAT
    CCTGGAGAGA GGGGCCCCCC TGGTCGTGCA GGTCTTCCTG GAGCTGATGG CTTGCCCGGA
    CCACCAGGAA CCATGCTGAT GTTGCCCTTC CGCTTCTCTG GTGGTGGAGA TGGTGGATCT
    AAAGGACCCC AAGTGTCTGC CCAGGAATCA CAAGCCCAAG CTATTCTCCA ACAAGCAAGA
    CTGGCGTTGA GAGGGCCATC TGGTCCGATG GGTCTTACTG GAAGACCAGG CCCATTGGGT
    TCTCCAGGTC CTTCAGGATT AAAGGGAGAG TCAGGAGATT TGGGACCTCA GGGGCCACGT
    GGGCCACAAG GTTCTCCCGG TCCATTAGGA AAACCAGGAC GAAGGGGACG AGCTGGTAGT
    GATGGTGCTC GGGGAATGCC TGGACAAACA GGACCAAAGG GTGACCGAGG CTTTGACGGG
    TTGGGCGGTT TGCCAGGAGA AAAGGGACAT CGTGGTGAGC CTGGTCCACA AGGTCCACCA
    GGGCCACATG GAGAAGACGG AGAAAGGGGT GATGATGGAG ACGTTGGACC TAGAGGGTTA
    CCAGGTGAAC CTGGACCCCG AGGGTTGCTT GGACCTAAAG GACCTCCTGG CCCACCGGGA
    ATCACCGGTG TTCCTGGAAT GGACGGTCAA GTTGGACCCA AAGGTAGTGT GGGACCTCAA
    GGTGAGCCAG GACCACCTGG GCAGCAAGGA AACCCAGGAG CACAGGGTCT TCCAGGTCCT
    CAGGGAGCTA TTGGACCACC AGGAGAGAAG GGACCACTGG GAAAATCAGG TCTTCCCGGA
    ATGCCCGGAG CTGATGGACC TCCCGGTCAC CCCGGGAAAG AAGGACCCTC AGGAGAGAAA
    GGAACCCTGG GTCCTGCAGG GCCACAGGGA CCAATTGGAT ACCCAGGACC ACGTGGTGTT
    AAGGGAGCTG ATGGAATTCG TGGTCTAAAG GGAACAAAAG GAGAAAAGGG TGAAGATGGA
    TTCCCTGGAT TTAAGGGTGA TATGGGCACC AAAGGAGACA GAGGTGAAAT CGGTCCTCCT
    GGTCCTAGAG GTGAAGATGG TCCTGAAGGT CCAAAGGGTC GAGGTGGTCC ATCTGGAGAA
    ATAGGTCCAC TTGGTCCTGG CGGTGAAAAG GGAAAGCTTG GTGTTCCTGG TCTACCAGGT
    TATCCAGGAA GACAAGGTCC AAAGGGTTCT ATCGGATTCC CAGGATTCGC AGGAGCAAAC
    GGAGAGAAAG GAACAAGGGG TACCCCTGGC AAACCGGGTC CAAGGGGGCA ACGAGGTCCA
    ACGGGTCCAC GTGGTGAAAG AGGTCCAAGG GGAGCTACAG GCAAGCCAGG CCCCAAGGGT
    AACTCCGGAG GGGATGGCCC AGCAGGTCCA CCTGGTGAAA GGGGACCACC TGGACCTCAA
    GGACCCACTG GATTTGCAGG ACCGAAGGGC CCCCCTGGTC CACCAGGGAA AGATGGACTT
    CCCGGCCACC CTGGACAGAG AGGAGAGACT GGATTCCAAG GAAAGACTGG CCCTCCTGGT
    CCCCCTGGCG TTGTCGGTCC TCAGGGTCCA ACTGGAGAGA CTGGCCCAAT GGGTGAAAGA
    GGACATCCTG GTCCTCCAGG TCCTCCTGGT GAGCAAGGTT TACCTGGTGT GGCTGGAAAA
    GAAGGATCCA AGGGAGACCC CGGTCCTGCT GGTTTGCCTG GAAAAGATGG ACCTCCAGGT
    CTAAGAGGTT TTACTGGAGA AAGAGGTCTC CCCGGTCCGA TTGGTGTTCC TGGACTGAAA
    GGCAATGAGG GTCCTCCTGG TCCACCTGGC CCAGCTGGTT CCCCCGGTGA AAGAGGAGCT
    GCGGGTCCAG CCGGACCTAT TGGACTGCCT GGAAGACCTG GCCCTCAGGG GCCACCAGGA
    CCTGCCGGCG AGAAGGGTAC ACCAGGTGAG AAAGGTCCCC AGGGACCTGC TGGACGTGAT
    GGTATCCAAG GCCCCGTTGG ACTTCCAGGC CCAGCTGGAC CTGTAGGGCC ACCTGGTGAA
    GATGGTGATA AGGGTGAAAT CGGAGAGCCT GGGCAAAAAG GAAACAGAGG AGATAAAGGA
    GAGCAGGGTC CCCCAGGTCC ACCTGGCCCA CAAGGTCCCA TTGGTCAGCC TGGCCCATCA
    GGATCTGATG GAGAGCCAGG CCCCAGGGGT CAACAAGGTC TTTTTGGACA AAAAGGTGAT
    GAAGGACCTA GAGGTTTTGC TGGACCACCA GGACCTGTTG GACTGCAGGG TTTACCTGGA
    CCACCTGGTG AGAAGGGAGA AACCGGAGAC GTTGGTCAGA TGGGTCCTCC TGGTCCCCCT
    GGTCCTAGAG GTCCTTCTGG CTCCCCTGGA GCTGATGGCC CACAAGGTCC TCCTGGAGGA
    ATTGGCAACC CTGGTCCTGT AGGAGAGAAG GGTGACCCTG GAGAGTCTGG GGAACCTGGT
    CTTCCTGGTG AACTTGGACC ACCGGGCCCT AAAGGTGAAA GAGGTGAAAA GGGAGAAGCG
    GGTCTTCAAG GAACTTCTGG CCCTCCAGGT CCTAAAGGTC CTCCAGGTGA TGATGGTCCA
    AAAGGTAGCC CTGGCCCAGT TGGATTTCCT GGTGATCCTG GTCCTCCTGG AGAATCTGGT
    CCTGCAGGTC AAGATGGTCC ACCTGGTGAT AAGGGAGAGG ACGGTGAATC TGGACAGCCA
    GGGTCACCAG GTCCTACTGG AGAGCCTGGG CCTTCAGGAC CACCTGGAAA GAGGGGTCCT
    CCTGGTCCAA CAGGTCCTGA AGGACGTCAA GGAGAAAAAG GAGCAAAGGG TGAATCAGGT
    TTGGAAGGAC CTCCTGGTAA GACTGGTCCT ATTGGTCCCC AAGGACCTCC CGGAAAACCT
    GGACCTGAAG GCCTTCGAGG AATTCCAGGC CCTGTTGGTG AACAAGGTCT CCCTGGTTCA
    CCTGGACCTG ATGGCCCTCC CGGACCTATG GGTCCACCAG GTATACCAGG TCTGAAAGGA
    GACTCAGGTC CAAAAGGAGA AAAGGGTCAT CCAGGCTTGA TAGGTCTCAT TGGACCTCCT
    GGTGAAACTA AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012824630.1
    CDS343..4917
    Translation

    Target ORF information:

    RefSeq Version XM_012969176.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type V, alpha 1 (col5a1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012969176.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTATGG ATTCCCACAC ACGATGGAAA AGACGGAATT GGACGACAGA GTGGCCCCTC 
    CTTGTAGCTT TAGCCTTGTC TATATGTTCC CGGTTCAGCG CAGCTGAACC GGCGGACCTG
    TTGAAGGCCT TAGAATTTCA CAATTTACCA GACGGTATCA AAAAAACGAC GGGCTTCTGC
    GCCTACCGCA AATCTTCAGA TGGACCAGAC GTGGCATTTA GAGTGTCCAA AGAGGCCCAG
    CTTAGCGCTC CGACCAATCA ATTGTTCTCC GATGTCGCAT TCCCAGAAGA TTTCTCCTTG
    TTGGCAACTG TGAAACCCAA AAAAGGAAGC CATTCCTACC TGCTCTCCAT CTACAATGAG
    CAAGGAACCC TGCAGATCGG CCTGACGCTG GGCTGGGAAC CGATGTTCGT CTATGAAGAC
    CACCAGGGAC ACCCACCCCC CGAGGATTAC CCCATCTTTA GAAACATTAA TCTGTCAGAT
    GGGAAGTGGC ACCGGGTAGC TTTCAGCGTG CACAAGAAGA CGATCACCCT GTTCCTGGAC
    TGCGTCAAGA AGGCCACCCT GCCCTTGAAA AGAAGCGACG GCCCCATTAT AGACACGAAC
    GGCATCGTCG TGTTCGGCAC CAGGATCCTG GACGAGCAAG TCTTTGAGGG AGACATTCAA
    CAGCTGATGA TCGTGCCCGA CGCCCGTGCT GCACATGATT ATTGTGAACA CTACACCCCC
    GACTGTGACA CCGCCATTCC AGATGCACCG CAGTCTCAGG ATCCCAACCA AGATGAATAT
    TACCCAGAAG CTGCTGAAGC CGAAGGCGAG GGTTATTACT ATGAATATCC TTACTACGAA
    GATGAGAAGA CAGAAACTGC AACTACCAAG CCCACGCCTG AGAAAGCCGA GGTACTAAGG
    CCCGAGACAG AAATCGAAGA GGAGGAAACA CCCCCACCAA CTGAAGCCCC TGCTATTCCC
    GTCACTGATG CTCCACGATT TGAGACCAGG GAAGAGAGCA GGGTAGAGAG CAGGGTAGAG
    AGCAGAGTGG ATAACCCCAC CACCGATGAC TACAACTATG AAACCGTACA GGAAGAATAT
    TACACCGCCT CTCCTTATGA AGATGTCAAC TACTCTGAAG GAGAGGAAAC AGGAATTGAG
    ACTGGGACTA CTGGTGAAGG CCTCATCGAG GGAGAAGGAG AAATTTTGAC CAAAACTGTC
    ATCACAAGCA GCTCATCCAA TGGCTCATCG GTCATCAGCA GTGGAAGTTC CAAAAAAGAA
    GGCAGTACCG ACGGAGATTT CACAGAAGTG ACCATACAGG AGTACGACCA AGGGAAAGAT
    ACTTACTATG ACATCTACGA TGATAAGCTT CTCACTGACA CTTCAGACGG AATTGGTGGG
    CAGCCAGCTG AACAAGATCA ATTTGGCATT TCAGGTGGGG CCAGAGGTGA GAAAGGACAG
    AAGGGAGAGC CAGCCATCAT TGAACCCGGA ATGCTGATCG AAGGACCACC AGGCCCAGAA
    GGTCCAGCTG GTTATCCCGG TCCTCCTGGT ACCTCAGGTC CTCCTGGTCC AGCTGGAGAT
    CCTGGAGAGA GGGGCCCCCC TGGTCGTGCA GGTCTTCCTG GAGCTGATGG CTTGCCCGGA
    CCACCAGGAA CCATGCTGAT GTTGCCCTTC CGCTTCTCTG GTGGTGGAGA TGGTGGATCT
    AAAGGACCCC AAGTGTCTGC CCAGGAATCA CAAGCCCAAG CTATTCTCCA ACAAGCAAGA
    CTGGCGTTGA GAGGGCCATC TGGTCCGATG GGTCTTACTG GAAGACCAGG CCCATTGGGT
    TCTCCAGGTC CTTCAGGATT AAAGGGAGAG TCAGGAGATT TGGGACCTCA GGGGCCACGT
    GGGCCACAAG GTTCTCCCGG TCCATTAGGA AAACCAGGAC GAAGGGGACG AGCTGGTAGT
    GATGGTGCTC GGGGAATGCC TGGACAAACA GGACCAAAGG GTGACCGAGG CTTTGACGGG
    TTGGGCGGTT TGCCAGGAGA AAAGGGACAT CGTGGTGAGC CTGGTCCACA AGGTCCACCA
    GGGCCACATG GAGAAGACGG AGAAAGGGGT GATGATGGAG ACGTTGGACC TAGAGGGTTA
    CCAGGTGAAC CTGGACCCCG AGGGTTGCTT GGACCTAAAG GACCTCCTGG CCCACCGGGA
    ATCACCGGTG TTCCTGGAAT GGACGGTCAA GTTGGACCCA AAGGTAGTGT GGGACCTCAA
    GGTGAGCCAG GACCACCTGG GCAGCAAGGA AACCCAGGAG CACAGGGTCT TCCAGGTCCT
    CAGGGAGCTA TTGGACCACC AGGAGAGAAG GGACCACTGG GAAAATCAGG TCTTCCCGGA
    ATGCCCGGAG CTGATGGACC TCCCGGTCAC CCCGGGAAAG AAGGACCCTC AGGAGAGAAA
    GGAACCCTGG GTCCTGCAGG GCCACAGGGA CCAATTGGAT ACCCAGGACC ACGTGGTGTT
    AAGGGAGCTG ATGGAATTCG TGGTCTAAAG GGAACAAAAG GAGAAAAGGG TGAAGATGGA
    TTCCCTGGAT TTAAGGGTGA TATGGGCACC AAAGGAGACA GAGGTGAAAT CGGTCCTCCT
    GGTCCTAGAG GTGAAGATGG TCCTGAAGGT CCAAAGGGTC GAGGTGGTCC ATCTGGAGAA
    ATAGGTCCAC TTGGTCCTGG CGGTGAAAAG GGAAAGCTTG GTGTTCCTGG TCTACCAGGT
    TATCCAGGAA GACAAGGTCC AAAGGGTTCT ATCGGATTCC CAGGATTCGC AGGAGCAAAC
    GGAGAGAAAG GAACAAGGGG TACCCCTGGC AAACCGGGTC CAAGGGGGCA ACGAGGTCCA
    ACGGGTCCAC GTGGTGAAAG AGGTCCAAGG GGAGCTACAG GCAAGCCAGG CCCCAAGGGT
    AACTCCGGAG GGGATGGCCC AGCAGGTCCA CCTGGTGAAA GGGGACCACC TGGACCTCAA
    GGACCCACTG GATTTGCAGG ACCGAAGGGC CCCCCTGGTC CACCAGGGAA AGATGGACTT
    CCCGGCCACC CTGGACAGAG AGGAGAGACT GGATTCCAAG GAAAGACTGG CCCTCCTGGT
    CCCCCTGGCG TTGTCGGTCC TCAGGGTCCA ACTGGAGAGA CTGGCCCAAT GGGTGAAAGA
    GGACATCCTG GTCCTCCAGG TCCTCCTGGT GAGCAAGGTT TACCTGGTGT GGCTGGAAAA
    GAAGGATCCA AGGGAGACCC CGGTCCTGCT GGTTTGCCTG GAAAAGATGG ACCTCCAGGT
    CTAAGAGGTT TTACTGGAGA AAGAGGTCTC CCCGGTCCGA TTGGTGTTCC TGGACTGAAA
    GGCAATGAGG GTCCTCCTGG TCCACCTGGC CCAGCTGGTT CCCCCGGTGA AAGAGGAGCT
    GCGGGTCCAG CCGGACCTAT TGGACTGCCT GGAAGACCTG GCCCTCAGGG GCCACCAGGA
    CCTGCCGGCG AGAAGGGTAC ACCAGGTGAG AAAGGTCCCC AGGGACCTGC TGGACGTGAT
    GGTATCCAAG GCCCCGTTGG ACTTCCAGGC CCAGCTGGAC CTGTAGGGCC ACCTGGTGAA
    GATGGTGATA AGGGTGAAAT CGGAGAGCCT GGGCAAAAAG GAAACAGAGG AGATAAAGGA
    GAGCAGGGTC CCCCAGGTCC ACCTGGCCCA CAAGGTCCCA TTGGTCAGCC TGGCCCATCA
    GGATCTGATG GAGAGCCAGG CCCCAGGGGT CAACAAGGTC TTTTTGGACA AAAAGGTGAT
    GAAGGACCTA GAGGTTTTGC TGGACCACCA GGACCTGTTG GACTGCAGGG TTTACCTGGA
    CCACCTGGTG AGAAGGGAGA AACCGGAGAC GTTGGTCAGA TGGGTCCTCC TGGTCCCCCT
    GGTCCTAGAG GTCCTTCTGG CTCCCCTGGA GCTGATGGCC CACAAGGTCC TCCTGGAGGA
    ATTGGCAACC CTGGTCCTGT AGGAGAGAAG GGTGACCCTG GAGAGTCTGG GGAACCTGGT
    CTTCCTGGTG AACTTGGACC ACCGGGCCCT AAAGGTGAAA GAGGTGAAAA GGGAGAAGCG
    GGTCTTCAAG GAACTTCTGG CCCTCCAGGT CCTAAAGGTC CTCCAGGTGA TGATGGTCCA
    AAAGGTAGCC CTGGCCCAGT TGGATTTCCT GGTGATCCTG GTCCTCCTGG AGAATCTGGT
    CCTGCAGGTC AAGATGGTCC ACCTGGTGAT AAGGGAGAGG ACGGTGAATC TGGACAGCCA
    GGGTCACCAG GTCCTACTGG AGAGCCTGGG CCTTCAGGAC CACCTGGAAA GAGGGGTCCT
    CCTGGTCCAA CAGGTCCTGA AGGACGTCAA GGAGAAAAAG GAGCAAAGGG TGAATCAGGT
    TTGGAAGGAC CTCCTGGTAA GACTGGTCCT ATTGGTCCCC AAGGACCTCC CGGAAAACCT
    GGACCTGAAG GCCTTCGAGG AATTCCAGGC CCTGTTGGTG AACAAGGTCT CCCTGGTTCA
    CCTGGACCTG ATGGCCCTCC CGGACCTATG GGTCCACCAG GTATACCAGG TCTGAAAGGA
    GACTCAGGTC CAAAAGGAGA AAAGGGTCAT CCAGGCTTGA TAGGTCTCAT TGGACCTCCT
    GGTGAAACTA AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.