col5a1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	col5a1
Entrez Gene ID	100038277
Full Name	collagen, type V, alpha 1
General protein information	Preferred Names collagen, type V, alpha 1 Names collagen alpha-1(V) chain
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004916645.3	XP_004916702.1	collagen alpha-1(V) chain isoform X1
XM_012969176.2	XP_012824630.1	collagen alpha-1(V) chain isoform X3
XM_012969176.3	XP_012824630.1	collagen alpha-1(V) chain isoform X3
XM_012969175.2	XP_012824629.1	collagen alpha-1(V) chain isoform X2
XM_004916645.4	XP_004916702.1	collagen alpha-1(V) chain isoform X1
XM_012969175.3	XP_012824629.1	collagen alpha-1(V) chain isoform X2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Col5a1	NP_604447.1
Homo sapiens (human)	COL5A1	NP_001265003.1
Mus musculus (house mouse)	Col5a1	NP_056549.2
Gallus gallus (chicken)	COL5A1	NP_990121.1
Xenopus tropicalis (tropical clawed frog)	col5a1	XP_004916702.1
Danio rerio (zebrafish)	col5a1	XP_002665924.2

Gene Ontology

The following col5a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the col5a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa14033	XM_004916645.4 Latest version!	Xenopus tropicalis collagen, type V, alpha 1 (col5a1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa30187	XM_012969175.3 Latest version!	Xenopus tropicalis collagen, type V, alpha 1 (col5a1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa30188	XM_012969176.3 Latest version!	Xenopus tropicalis collagen, type V, alpha 1 (col5a1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OXa30188	XM_012969176.2	Xenopus tropicalis collagen, type V, alpha 1 (col5a1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa14033	XM_004916645.3	Xenopus tropicalis collagen, type V, alpha 1 (col5a1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OXa30187	XM_012969175.2	Xenopus tropicalis collagen, type V, alpha 1 (col5a1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

col5a1 ( XM_004916645.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916702.1 Xenopus tropicalis collagen, type V, alpha 1 (col5a1), transcript variant X1, mRNA.

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CloneID	OXa14033
Clone ID Related Accession (Same CDS sequence)	XM_004916645.3 , XM_004916645.4
Accession Version	XM_004916645.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-1(V) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004916645.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTATGG ATTCCCACAC ACGATGGAAA AGACGGAATT GGACGACAGA GTGGCCCCTC 
CTTGTAGCTT TAGCCTTGTC TATATGTTCC CGGTTCAGCG CAGCTGAACC GGCGGACCTG 
TTGAAGGCCT TAGAATTTCA CAATTTACCA GACGGTATCA AAAAAACGAC GGGCTTCTGC 
GCCTACCGCA AATCTTCAGA TGGACCAGAC GTGGCATTTA GAGTGTCCAA AGAGGCCCAG 
CTTAGCGCTC CGACCAATCA ATTGTTCTCC GATGTCGCAT TCCCAGAAGA TTTCTCCTTG 
TTGGCAACTG TGAAACCCAA AAAAGGAAGC CATTCCTACC TGCTCTCCAT CTACAATGAG 
CAAGGAACCC TGCAGATCGG CCTGACGCTG GGCTGGGAAC CGATGTTCGT CTATGAAGAC 
CACCAGGGAC ACCCACCCCC CGAGGATTAC CCCATCTTTA GAAACATTAA TCTGTCAGAT 
GGGAAGTGGC ACCGGGTAGC TTTCAGCGTG CACAAGAAGA CGATCACCCT GTTCCTGGAC 
TGCGTCAAGA AGGCCACCCT GCCCTTGAAA AGAAGCGACG GCCCCATTAT AGACACGAAC 
GGCATCGTCG TGTTCGGCAC CAGGATCCTG GACGAGCAAG TCTTTGAGGG AGACATTCAA 
CAGCTGATGA TCGTGCCCGA CGCCCGTGCT GCACATGATT ATTGTGAACA CTACACCCCC 
GACTGTGACA CCGCCATTCC AGATGCACCG CAGTCTCAGG ATCCCAACCA AGATGAATAT 
TACCCAGAAG CTGCTGAAGC CGAAGGCGAG GGTTATTACT ATGAATATCC TTACTACGAA 
GATGAGAAGA CAGAAACTGC AACTACCAAG CCCACGCCTG AGAAAGCCGA GGTACTAAGG 
CCCGAGACAG AAATCGAAGA GGAGGAAACA CCCCCACCAA CTGAAGCCCC TGCTATTCCC 
GTCACTGATG CTCCACGATT TGAGACCAGG GAAGAGAGCA GGGTAGAGAG CAGGGTAGAG 
AGCAGAGTGG ATAACCCCAC CACCGATGAC TACAACTATG AAACCGTACA GGAAGAATAT 
TACACCGCCT CTCCTTATGA AGATGTCAAC TACTCTGAAG GAGAGGAAAC AGGAATTGAG 
ACTGGGACTA CTGGTGAAGG CCTCATCGAG GGAGAAGGAG AAATTTTGAC CAAAACTGTC 
ATCACAAGCA GCTCATCCAA TGGCTCATCG GTCATCAGCA GTGGAAGTTC CAAAAAAGAA 
GGCAGTACCG ACGGAGATTT CACAGAAGTG ACCATACAGG AGTACGACCA AGGGAAAGAT 
ACTTACTATG ACATCTACGA TGATAAGCTT CTCACTGACA CTTCAGACGG AATTGGTGGG 
CAGCCAGCTG AACAAGATCA ATTTGGCATT TCAGGTGGGG CCAGAGGTGA GAAAGGACAG 
AAGGGAGAGC CAGCCATCAT TGAACCCGGA ATGCTGATCG AAGGACCACC AGGCCCAGAA 
GGTCCAGCTG GTTATCCCGG TCCTCCTGGT ACCTCAGGTC CTCCTGGTCC AGCTGGAGAT 
CCTGGAGAGA GGGGCCCCCC TGGTCGTGCA GGTCTTCCTG GAGCTGATGG CTTGCCCGGA 
CCACCAGGAA CCATGCTGAT GTTGCCCTTC CGCTTCTCTG GTGGTGGAGA TGGTGGATCT 
AAAGGACCCC AAGTGTCTGC CCAGGAATCA CAAGCCCAAG CTATTCTCCA ACAAGCAAGA 
CTGGCGTTGA GAGGGCCATC TGGTCCGATG GGTCTTACTG GAAGACCAGG CCCATTGGGT 
TCTCCAGGTC CTTCAGGATT AAAGGGAGAG TCAGGAGATT TGGGACCTCA GGGGCCACGT 
GGGCCACAAG GTTCTCCCGG TCCATTAGGA AAACCAGGAC GAAGGGGACG AGCTGGTAGT 
GATGGTGCTC GGGGAATGCC TGGACAAACA GGACCAAAGG GTGACCGAGG CTTTGACGGG 
TTGGGCGGTT TGCCAGGAGA AAAGGGACAT CGTGGTGAGC CTGGTCCACA AGGTCCACCA 
GGGCCACATG GAGAAGACGG AGAAAGGGGT GATGATGGAG ACGTTGGACC TAGAGGGTTA 
CCAGGTGAAC CTGGACCCCG AGGGTTGCTT GGACCTAAAG GACCTCCTGG CCCACCGGGA 
ATCACCGGTG TTCCTGGAAT GGACGGTCAA GTTGGACCCA AAGGTAGTGT GGGACCTCAA 
GGTGAGCCAG GACCACCTGG GCAGCAAGGA AACCCAGGAG CACAGGGTCT TCCAGGTCCT 
CAGGGAGCTA TTGGACCACC AGGAGAGAAG GGACCACTGG GAAAATCAGG TCTTCCCGGA 
ATGCCCGGAG CTGATGGACC TCCCGGTCAC CCCGGGAAAG AAGGACCCTC AGGAGAGAAA 
GGAACCCTGG GTCCTGCAGG GCCACAGGGA CCAATTGGAT ACCCAGGACC ACGTGGTGTT 
AAGGGAGCTG ATGGAATTCG TGGTCTAAAG GGAACAAAAG GAGAAAAGGG TGAAGATGGA 
TTCCCTGGAT TTAAGGGTGA TATGGGCACC AAAGGAGACA GAGGTGAAAT CGGTCCTCCT 
GGTCCTAGAG GTGAAGATGG TCCTGAAGGT CCAAAGGGTC GAGGTGGTCC ATCTGGAGAA 
ATAGGTCCAC TTGGTCCTGG CGGTGAAAAG GGAAAGCTTG GTGTTCCTGG TCTACCAGGT 
TATCCAGGAA GACAAGGTCC AAAGGGTTCT ATCGGATTCC CAGGATTCGC AGGAGCAAAC 
GGAGAGAAAG GAACAAGGGG TACCCCTGGC AAACCGGGTC CAAGGGGGCA ACGAGGTCCA 
ACGGGTCCAC GTGGTGAAAG AGGTCCAAGG GGAGCTACAG GCAAGCCAGG CCCCAAGGGT 
AACTCCGGAG GGGATGGCCC AGCAGGTCCA CCTGGTGAAA GGGGACCACC TGGACCTCAA 
GGACCCACTG GATTTGCAGG ACCGAAGGGC CCCCCTGGTC CACCAGGGAA AGATGGACTT 
CCCGGCCACC CTGGACAGAG AGGAGAGACT GGATTCCAAG GAAAGACTGG CCCTCCTGGT 
CCCCCTGGCG TTGTCGGTCC TCAGGGTCCA ACTGGAGAGA CTGGCCCAAT GGGTGAAAGA 
GGACATCCTG GTCCTCCAGG TCCTCCTGGT GAGCAAGGTT TACCTGGTGT GGCTGGAAAA 
GAAGGATCCA AGGGAGACCC CGGTCCTGCT GGTTTGCCTG GAAAAGATGG ACCTCCAGGT 
CTAAGAGGTT TTACTGGAGA AAGAGGTCTC CCCGGTCCGA TTGGTGTTCC TGGACTGAAA 
GGCAATGAGG GTCCTCCTGG TCCACCTGGC CCAGCTGGTT CCCCCGGTGA AAGAGGAGCT 
GCGGGTCCAG CCGGACCTAT TGGACTGCCT GGAAGACCTG GCCCTCAGGG GCCACCAGGA 
CCTGCCGGCG AGAAGGGTAC ACCAGGTGAG AAAGGTCCCC AGGGACCTGC TGGACGTGAT 
GGTATCCAAG GCCCCGTTGG ACTTCCAGGC CCAGCTGGAC CTGTAGGGCC ACCTGGTGAA 
GATGGTGATA AGGGTGAAAT CGGAGAGCCT GGGCAAAAAG GAAACAGAGG AGATAAAGGA 
GAGCAGGGTC CCCCAGGTCC ACCTGGCCCA CAAGGTCCCA TTGGTCAGCC TGGCCCATCA 
GGATCTGATG GAGAGCCAGG CCCCAGGGGT CAACAAGGTC TTTTTGGACA AAAAGGTGAT 
GAAGGACCTA GAGGTTTTGC TGGACCACCA GGACCTGTTG GACTGCAGGG TTTACCTGGA 
CCACCTGGTG AGAAGGGAGA AACCGGAGAC GTTGGTCAGA TGGGTCCTCC TGGTCCCCCT 
GGTCCTAGAG GTCCTTCTGG CTCCCCTGGA GCTGATGGCC CACAAGGTCC TCCTGGAGGA 
ATTGGCAACC CTGGTCCTGT AGGAGAGAAG GGTGACCCTG GAGAGTCTGG GGAACCTGGT 
CTTCCTGGTG AACTTGGACC ACCGGGCCCT AAAGGTGAAA GAGGTGAAAA GGGAGAAGCG 
GGTCTTCAAG GAACTTCTGG CCCTCCAGGT CCTAAAGGTC CTCCAGGTGA TGATGGTCCA 
AAAGGTAGCC CTGGCCCAGT TGGATTTCCT GGTGATCCTG GTCCTCCTGG AGAATCTGGT 
CCTGCAGGTC AAGATGGTCC ACCTGGTGAT AAGGGAGAGG ACGGTGAATC TGGACAGCCA 
GGGTCACCAG GTCCTACTGG AGAGCCTGGG CCTTCAGGAC CACCTGGAAA GAGGGGTCCT 
CCTGGTCCAA CAGGTCCTGA AGGACGTCAA GGAGAAAAAG GAGCAAAGGG TGAATCAGGT 
TTGGAAGGAC CTCCTGGTAA GACTGGTCCT ATTGGTCCCC AAGGACCTCC CGGAAAACCT 
GGACCTGAAG GCCTTCGAGG AATTCCAGGC CCTGTTGGTG AACAAGGTCT CCCTGGTTCA 
CCTGGACCTG ATGGCCCTCC CGGACCTATG GGTCCACCAG GTATACCAGG TCTGAAAGGA 
GACTCAGGTC CAAAAGGAGA AAAGGGTCAT CCAGGCTTGA TAGGTCTCAT TGGACCTCCT 
GGTGAACAGG GAGAAAAGGG AGACCGTGGT TTGCCAGGCC CCCAGGGACC ACATGGTGCT 
AAAGGAGAAC AGGGTATTCT TGGTCCATCT GGTCCTGTTG GTCCACCTGG TCCTCCAGGC 
TTACCGGGTG GTCCTGGTCC AAAAGGTGCT AAAGGTTCAT CGGGTCCAAC TGGTCCAAAG 
GGTGAATCTG GAATCCCTGG TCCACCAGGT CCTCCAGGAC CTCCAGGCGA GGTGATCCAG 
CCTCTGCCTA TCCATTCCTC AAAGAGGACA AGACGTAGCA TTGATGCAAG TCAAGTAGTG 
GACGATGGAA ATGCAGACAA CTACATGGAC TATGCCGATG GAATGGAGGA AATATTTGGT 
TCTCTCAACT CCCTAAAACA GGAAATTGAA CAAATGAAAC ACCCACTGGG CAATCAGCAG 
AACCCAGCAA GGACTTGCAA GGACCTGCTT CTGTGCCATC CTGAACTCCC AGATGGGGAA 
TATTGGATTG ACCCAAATCA AGGATGCTCT AGAGACTCGT TCAAGGTTTT CTGTAATTTC 
ACAGCCGGTG GAGAATCTTG TATCTTCCCC GACAAGAAGT CTGAAGGAGC TAGAATTACA 
TCTTGGCCCA AGGAAAATCC AGGCTCCTGG TTCAGTGAAT TCAAGCGTGG TAAACTGTTG 
TCCTACGTTG ATTCAAATGG CAACCCCATT GGAGTGGTGC AGATGACCTT CATGAAGCTT 
CTGAGTGCTT CGGTGAAACA GAATATCACC TACAACTGCT ACCAGTCCGT GGCATGGCAC 
GACGCCACCA CCAACAGCTA CGACAAGGCC ATTCGCTTCC TGGGGTCCAA CGACGAAGAA 
ATGTCCTACG ACAACAATCC TTCCATTCGA GCCATTATGG ATGGATGTGC GTCAAAGAAA 
GGTTATCAGA AGACAGTTCT GGAACTCGAC ACGTCCAAAG TGGAACAGGC CCCCATTGTG 
GATATTATGT TCAACGACTT CGGAGATCCA GTACAAAAAT TCGGCTTTGA GGTGGGACCA 
GTGTGTTTCA TGGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916702.1
CDS	343..5940
Translation	MSMDSHTRWKRRNWTTEWPLLVALALSICSRFSAAEPADLLKALEFHNLPDGIKKTTGFCAYRKSSDGPDVAFRVSKEAQLSAPTNQLFSDVAFPEDFSLLATVKPKKGSHSYLLSIYNEQGTLQIGLTLGWEPMFVYEDHQGHPPPEDYPIFRNINLSDGKWHRVAFSVHKKTITLFLDCVKKATLPLKRSDGPIIDTNGIVVFGTRILDEQVFEGDIQQLMIVPDARAAHDYCEHYTPDCDTAIPDAPQSQDPNQDEYYPEAAEAEGEGYYYEYPYYEDEKTETATTKPTPEKAEVLRPETEIEEEETPPPTEAPAIPVTDAPRFETREESRVESRVESRVDNPTTDDYNYETVQEEYYTASPYEDVNYSEGEETGIETGTTGEGLIEGEGEILTKTVITSSSSNGSSVISSGSSKKEGSTDGDFTEVTIQEYDQGKDTYYDIYDDKLLTDTSDGIGGQPAEQDQFGISGGARGEKGQKGEPAIIEPGMLIEGPPGPEGPAGYPGPPGTSGPPGPAGDPGERGPPGRAGLPGADGLPGPPGTMLMLPFRFSGGGDGGSKGPQVSAQESQAQAILQQARLALRGPSGPMGLTGRPGPLGSPGPSGLKGESGDLGPQGPRGPQGSPGPLGKPGRRGRAGSDGARGMPGQTGPKGDRGFDGLGGLPGEKGHRGEPGPQGPPGPHGEDGERGDDGDVGPRGLPGEPGPRGLLGPKGPPGPPGITGVPGMDGQVGPKGSVGPQGEPGPPGQQGNPGAQGLPGPQGAIGPPGEKGPLGKSGLPGMPGADGPPGHPGKEGPSGEKGTLGPAGPQGPIGYPGPRGVKGADGIRGLKGTKGEKGEDGFPGFKGDMGTKGDRGEIGPPGPRGEDGPEGPKGRGGPSGEIGPLGPGGEKGKLGVPGLPGYPGRQGPKGSIGFPGFAGANGEKGTRGTPGKPGPRGQRGPTGPRGERGPRGATGKPGPKGNSGGDGPAGPPGERGPPGPQGPTGFAGPKGPPGPPGKDGLPGHPGQRGETGFQGKTGPPGPPGVVGPQGPTGETGPMGERGHPGPPGPPGEQGLPGVAGKEGSKGDPGPAGLPGKDGPPGLRGFTGERGLPGPIGVPGLKGNEGPPGPPGPAGSPGERGAAGPAGPIGLPGRPGPQGPPGPAGEKGTPGEKGPQGPAGRDGIQGPVGLPGPAGPVGPPGEDGDKGEIGEPGQKGNRGDKGEQGPPGPPGPQGPIGQPGPSGSDGEPGPRGQQGLFGQKGDEGPRGFAGPPGPVGLQGLPGPPGEKGETGDVGQMGPPGPPGPRGPSGSPGADGPQGPPGGIGNPGPVGEKGDPGESGEPGLPGELGPPGPKGERGEKGEAGLQGTSGPPGPKGPPGDDGPKGSPGPVGFPGDPGPPGESGPAGQDGPPGDKGEDGESGQPGSPGPTGEPGPSGPPGKRGPPGPTGPEGRQGEKGAKGESGLEGPPGKTGPIGPQGPPGKPGPEGLRGIPGPVGEQGLPGSPGPDGPPGPMGPPGIPGLKGDSGPKGEKGHPGLIGLIGPPGEQGEKGDRGLPGPQGPHGAKGEQGILGPSGPVGPPGPPGLPGGPGPKGAKGSSGPTGPKGESGIPGPPGPPGPPGEVIQPLPIHSSKRTRRSIDASQVVDDGNADNYMDYADGMEEIFGSLNSLKQEIEQMKHPLGNQQNPARTCKDLLLCHPELPDGEYWIDPNQGCSRDSFKVFCNFTAGGESCIFPDKKSEGARITSWPKENPGSWFSEFKRGKLLSYVDSNGNPIGVVQMTFMKLLSASVKQNITYNCYQSVAWHDATTNSYDKAIRFLGSNDEEMSYDNNPSIRAIMDGCASKKGYQKTVLELDTSKVEQAPIVDIMFNDFGDPVQKFGFEVGPVCFMG

Target ORF information:

RefSeq Version	XM_004916645.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen, type V, alpha 1 (col5a1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916645.4

ORF Insert Sequence:

ATGAGTATGG ATTCCCACAC ACGATGGAAA AGACGGAATT GGACGACAGA GTGGCCCCTC 
CTTGTAGCTT TAGCCTTGTC TATATGTTCC CGGTTCAGCG CAGCTGAACC GGCGGACCTG 
TTGAAGGCCT TAGAATTTCA CAATTTACCA GACGGTATCA AAAAAACGAC GGGCTTCTGC 
GCCTACCGCA AATCTTCAGA TGGACCAGAC GTGGCATTTA GAGTGTCCAA AGAGGCCCAG 
CTTAGCGCTC CGACCAATCA ATTGTTCTCC GATGTCGCAT TCCCAGAAGA TTTCTCCTTG 
TTGGCAACTG TGAAACCCAA AAAAGGAAGC CATTCCTACC TGCTCTCCAT CTACAATGAG 
CAAGGAACCC TGCAGATCGG CCTGACGCTG GGCTGGGAAC CGATGTTCGT CTATGAAGAC 
CACCAGGGAC ACCCACCCCC CGAGGATTAC CCCATCTTTA GAAACATTAA TCTGTCAGAT 
GGGAAGTGGC ACCGGGTAGC TTTCAGCGTG CACAAGAAGA CGATCACCCT GTTCCTGGAC 
TGCGTCAAGA AGGCCACCCT GCCCTTGAAA AGAAGCGACG GCCCCATTAT AGACACGAAC 
GGCATCGTCG TGTTCGGCAC CAGGATCCTG GACGAGCAAG TCTTTGAGGG AGACATTCAA 
CAGCTGATGA TCGTGCCCGA CGCCCGTGCT GCACATGATT ATTGTGAACA CTACACCCCC 
GACTGTGACA CCGCCATTCC AGATGCACCG CAGTCTCAGG ATCCCAACCA AGATGAATAT 
TACCCAGAAG CTGCTGAAGC CGAAGGCGAG GGTTATTACT ATGAATATCC TTACTACGAA 
GATGAGAAGA CAGAAACTGC AACTACCAAG CCCACGCCTG AGAAAGCCGA GGTACTAAGG 
CCCGAGACAG AAATCGAAGA GGAGGAAACA CCCCCACCAA CTGAAGCCCC TGCTATTCCC 
GTCACTGATG CTCCACGATT TGAGACCAGG GAAGAGAGCA GGGTAGAGAG CAGGGTAGAG 
AGCAGAGTGG ATAACCCCAC CACCGATGAC TACAACTATG AAACCGTACA GGAAGAATAT 
TACACCGCCT CTCCTTATGA AGATGTCAAC TACTCTGAAG GAGAGGAAAC AGGAATTGAG 
ACTGGGACTA CTGGTGAAGG CCTCATCGAG GGAGAAGGAG AAATTTTGAC CAAAACTGTC 
ATCACAAGCA GCTCATCCAA TGGCTCATCG GTCATCAGCA GTGGAAGTTC CAAAAAAGAA 
GGCAGTACCG ACGGAGATTT CACAGAAGTG ACCATACAGG AGTACGACCA AGGGAAAGAT 
ACTTACTATG ACATCTACGA TGATAAGCTT CTCACTGACA CTTCAGACGG AATTGGTGGG 
CAGCCAGCTG AACAAGATCA ATTTGGCATT TCAGGTGGGG CCAGAGGTGA GAAAGGACAG 
AAGGGAGAGC CAGCCATCAT TGAACCCGGA ATGCTGATCG AAGGACCACC AGGCCCAGAA 
GGTCCAGCTG GTTATCCCGG TCCTCCTGGT ACCTCAGGTC CTCCTGGTCC AGCTGGAGAT 
CCTGGAGAGA GGGGCCCCCC TGGTCGTGCA GGTCTTCCTG GAGCTGATGG CTTGCCCGGA 
CCACCAGGAA CCATGCTGAT GTTGCCCTTC CGCTTCTCTG GTGGTGGAGA TGGTGGATCT 
AAAGGACCCC AAGTGTCTGC CCAGGAATCA CAAGCCCAAG CTATTCTCCA ACAAGCAAGA 
CTGGCGTTGA GAGGGCCATC TGGTCCGATG GGTCTTACTG GAAGACCAGG CCCATTGGGT 
TCTCCAGGTC CTTCAGGATT AAAGGGAGAG TCAGGAGATT TGGGACCTCA GGGGCCACGT 
GGGCCACAAG GTTCTCCCGG TCCATTAGGA AAACCAGGAC GAAGGGGACG AGCTGGTAGT 
GATGGTGCTC GGGGAATGCC TGGACAAACA GGACCAAAGG GTGACCGAGG CTTTGACGGG 
TTGGGCGGTT TGCCAGGAGA AAAGGGACAT CGTGGTGAGC CTGGTCCACA AGGTCCACCA 
GGGCCACATG GAGAAGACGG AGAAAGGGGT GATGATGGAG ACGTTGGACC TAGAGGGTTA 
CCAGGTGAAC CTGGACCCCG AGGGTTGCTT GGACCTAAAG GACCTCCTGG CCCACCGGGA 
ATCACCGGTG TTCCTGGAAT GGACGGTCAA GTTGGACCCA AAGGTAGTGT GGGACCTCAA 
GGTGAGCCAG GACCACCTGG GCAGCAAGGA AACCCAGGAG CACAGGGTCT TCCAGGTCCT 
CAGGGAGCTA TTGGACCACC AGGAGAGAAG GGACCACTGG GAAAATCAGG TCTTCCCGGA 
ATGCCCGGAG CTGATGGACC TCCCGGTCAC CCCGGGAAAG AAGGACCCTC AGGAGAGAAA 
GGAACCCTGG GTCCTGCAGG GCCACAGGGA CCAATTGGAT ACCCAGGACC ACGTGGTGTT 
AAGGGAGCTG ATGGAATTCG TGGTCTAAAG GGAACAAAAG GAGAAAAGGG TGAAGATGGA 
TTCCCTGGAT TTAAGGGTGA TATGGGCACC AAAGGAGACA GAGGTGAAAT CGGTCCTCCT 
GGTCCTAGAG GTGAAGATGG TCCTGAAGGT CCAAAGGGTC GAGGTGGTCC ATCTGGAGAA 
ATAGGTCCAC TTGGTCCTGG CGGTGAAAAG GGAAAGCTTG GTGTTCCTGG TCTACCAGGT 
TATCCAGGAA GACAAGGTCC AAAGGGTTCT ATCGGATTCC CAGGATTCGC AGGAGCAAAC 
GGAGAGAAAG GAACAAGGGG TACCCCTGGC AAACCGGGTC CAAGGGGGCA ACGAGGTCCA 
ACGGGTCCAC GTGGTGAAAG AGGTCCAAGG GGAGCTACAG GCAAGCCAGG CCCCAAGGGT 
AACTCCGGAG GGGATGGCCC AGCAGGTCCA CCTGGTGAAA GGGGACCACC TGGACCTCAA 
GGACCCACTG GATTTGCAGG ACCGAAGGGC CCCCCTGGTC CACCAGGGAA AGATGGACTT 
CCCGGCCACC CTGGACAGAG AGGAGAGACT GGATTCCAAG GAAAGACTGG CCCTCCTGGT 
CCCCCTGGCG TTGTCGGTCC TCAGGGTCCA ACTGGAGAGA CTGGCCCAAT GGGTGAAAGA 
GGACATCCTG GTCCTCCAGG TCCTCCTGGT GAGCAAGGTT TACCTGGTGT GGCTGGAAAA 
GAAGGATCCA AGGGAGACCC CGGTCCTGCT GGTTTGCCTG GAAAAGATGG ACCTCCAGGT 
CTAAGAGGTT TTACTGGAGA AAGAGGTCTC CCCGGTCCGA TTGGTGTTCC TGGACTGAAA 
GGCAATGAGG GTCCTCCTGG TCCACCTGGC CCAGCTGGTT CCCCCGGTGA AAGAGGAGCT 
GCGGGTCCAG CCGGACCTAT TGGACTGCCT GGAAGACCTG GCCCTCAGGG GCCACCAGGA 
CCTGCCGGCG AGAAGGGTAC ACCAGGTGAG AAAGGTCCCC AGGGACCTGC TGGACGTGAT 
GGTATCCAAG GCCCCGTTGG ACTTCCAGGC CCAGCTGGAC CTGTAGGGCC ACCTGGTGAA 
GATGGTGATA AGGGTGAAAT CGGAGAGCCT GGGCAAAAAG GAAACAGAGG AGATAAAGGA 
GAGCAGGGTC CCCCAGGTCC ACCTGGCCCA CAAGGTCCCA TTGGTCAGCC TGGCCCATCA 
GGATCTGATG GAGAGCCAGG CCCCAGGGGT CAACAAGGTC TTTTTGGACA AAAAGGTGAT 
GAAGGACCTA GAGGTTTTGC TGGACCACCA GGACCTGTTG GACTGCAGGG TTTACCTGGA 
CCACCTGGTG AGAAGGGAGA AACCGGAGAC GTTGGTCAGA TGGGTCCTCC TGGTCCCCCT 
GGTCCTAGAG GTCCTTCTGG CTCCCCTGGA GCTGATGGCC CACAAGGTCC TCCTGGAGGA 
ATTGGCAACC CTGGTCCTGT AGGAGAGAAG GGTGACCCTG GAGAGTCTGG GGAACCTGGT 
CTTCCTGGTG AACTTGGACC ACCGGGCCCT AAAGGTGAAA GAGGTGAAAA GGGAGAAGCG 
GGTCTTCAAG GAACTTCTGG CCCTCCAGGT CCTAAAGGTC CTCCAGGTGA TGATGGTCCA 
AAAGGTAGCC CTGGCCCAGT TGGATTTCCT GGTGATCCTG GTCCTCCTGG AGAATCTGGT 
CCTGCAGGTC AAGATGGTCC ACCTGGTGAT AAGGGAGAGG ACGGTGAATC TGGACAGCCA 
GGGTCACCAG GTCCTACTGG AGAGCCTGGG CCTTCAGGAC CACCTGGAAA GAGGGGTCCT 
CCTGGTCCAA CAGGTCCTGA AGGACGTCAA GGAGAAAAAG GAGCAAAGGG TGAATCAGGT 
TTGGAAGGAC CTCCTGGTAA GACTGGTCCT ATTGGTCCCC AAGGACCTCC CGGAAAACCT 
GGACCTGAAG GCCTTCGAGG AATTCCAGGC CCTGTTGGTG AACAAGGTCT CCCTGGTTCA 
CCTGGACCTG ATGGCCCTCC CGGACCTATG GGTCCACCAG GTATACCAGG TCTGAAAGGA 
GACTCAGGTC CAAAAGGAGA AAAGGGTCAT CCAGGCTTGA TAGGTCTCAT TGGACCTCCT 
GGTGAACAGG GAGAAAAGGG AGACCGTGGT TTGCCAGGCC CCCAGGGACC ACATGGTGCT 
AAAGGAGAAC AGGGTATTCT TGGTCCATCT GGTCCTGTTG GTCCACCTGG TCCTCCAGGC 
TTACCGGGTG GTCCTGGTCC AAAAGGTGCT AAAGGTTCAT CGGGTCCAAC TGGTCCAAAG 
GGTGAATCTG GAATCCCTGG TCCACCAGGT CCTCCAGGAC CTCCAGGCGA GGTGATCCAG 
CCTCTGCCTA TCCATTCCTC AAAGAGGACA AGACGTAGCA TTGATGCAAG TCAAGTAGTG 
GACGATGGAA ATGCAGACAA CTACATGGAC TATGCCGATG GAATGGAGGA AATATTTGGT 
TCTCTCAACT CCCTAAAACA GGAAATTGAA CAAATGAAAC ACCCACTGGG CAATCAGCAG 
AACCCAGCAA GGACTTGCAA GGACCTGCTT CTGTGCCATC CTGAACTCCC AGATGGGGAA 
TATTGGATTG ACCCAAATCA AGGATGCTCT AGAGACTCGT TCAAGGTTTT CTGTAATTTC 
ACAGCCGGTG GAGAATCTTG TATCTTCCCC GACAAGAAGT CTGAAGGAGC TAGAATTACA 
TCTTGGCCCA AGGAAAATCC AGGCTCCTGG TTCAGTGAAT TCAAGCGTGG TAAACTGTTG 
TCCTACGTTG ATTCAAATGG CAACCCCATT GGAGTGGTGC AGATGACCTT CATGAAGCTT 
CTGAGTGCTT CGGTGAAACA GAATATCACC TACAACTGCT ACCAGTCCGT GGCATGGCAC 
GACGCCACCA CCAACAGCTA CGACAAGGCC ATTCGCTTCC TGGGGTCCAA CGACGAAGAA 
ATGTCCTACG ACAACAATCC TTCCATTCGA GCCATTATGG ATGGATGTGC GTCAAAGAAA 
GGTTATCAGA AGACAGTTCT GGAACTCGAC ACGTCCAAAG TGGAACAGGC CCCCATTGTG 
GATATTATGT TCAACGACTT CGGAGATCCA GTACAAAAAT TCGGCTTTGA GGTGGGACCA 
GTGTGTTTCA TGGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col5a1 ( XM_012969175.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824629.1 Xenopus tropicalis collagen, type V, alpha 1 (col5a1), transcript variant X2, mRNA.

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CloneID	OXa30187
Clone ID Related Accession (Same CDS sequence)	XM_012969175.2 , XM_012969175.3
Accession Version	XM_012969175.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-1(V) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012969175.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTATGG ATTCCCACAC ACGATGGAAA AGACGGAATT GGACGACAGA GTGGCCCCTC 
CTTGTAGCTT TAGCCTTGTC TATATGTTCC CGGTTCAGCG CAGCTGAACC GGCGGACCTG 
TTGAAGGCCT TAGAATTTCA CAATTTACCA GACGGTATCA AAAAAACGAC GGGCTTCTGC 
GCCTACCGCA AATCTTCAGA TGGACCAGAC GTGGCATTTA GAGTGTCCAA AGAGGCCCAG 
CTTAGCGCTC CGACCAATCA ATTGTTCTCC GATGTCGCAT TCCCAGAAGA TTTCTCCTTG 
TTGGCAACTG TGAAACCCAA AAAAGGAAGC CATTCCTACC TGCTCTCCAT CTACAATGAG 
CAAGGAACCC TGCAGATCGG CCTGACGCTG GGCTGGGAAC CGATGTTCGT CTATGAAGAC 
CACCAGGGAC ACCCACCCCC CGAGGATTAC CCCATCTTTA GAAACATTAA TCTGTCAGAT 
GGGAAGTGGC ACCGGGTAGC TTTCAGCGTG CACAAGAAGA CGATCACCCT GTTCCTGGAC 
TGCGTCAAGA AGGCCACCCT GCCCTTGAAA AGAAGCGACG GCCCCATTAT AGACACGAAC 
GGCATCGTCG TGTTCGGCAC CAGGATCCTG GACGAGCAAG TCTTTGAGGG AGACATTCAA 
CAGCTGATGA TCGTGCCCGA CGCCCGTGCT GCACATGATT ATTGTGAACA CTACACCCCC 
GACTGTGACA CCGCCATTCC AGATGCACCG CAGTCTCAGG ATCCCAACCA AGATGAATAT 
TACCCAGAAG CTGCTGAAGC CGAAGGCGAG GGTTATTACT ATGAATATCC TTACTACGAA 
GATGAGAAGA CAGAAACTGC AACTACCAAG CCCACGCCTG AGAAAGCCGA GGTACTAAGG 
CCCGAGACAG AAATCGAAGA GGAGGAAACA CCCCCACCAA CTGAAGCCCC TGCTATTCCC 
GTCACTGATG CTCCACGATT TGAGACCAGG GAAGAGAGCA GGGTAGAGAG CAGGGTAGAG 
AGCAGAGTGG ATAACCCCAC CACCGATGAC TACAACTATG AAACCGTACA GGAAGAATAT 
TACACCGCCT CTCCTTATGA AGATGTCAAC TACTCTGAAG GAGAGGAAAC AGGAATTGAG 
ACTGGGACTA CTGGTGAAGG CCTCATCGAG GGAGAAGGAG AAATTTTGAC CAAAACTGTC 
ATCACAAGCA GCTCATCCAA TGGCTCATCG GTCATCAGCA GTGGAAGTTC CAAAAAAGAA 
GGCAGTACCG ACGGAGATTT CACAGAAGTG ACCATACAGG AGTACGACCA AGGGAAAGAT 
ACTTACTATG ACATCTACGA TGATAAGCTT CTCACTGACA CTTCAGACGG AATTGGTGGG 
CAGCCAGCTG AACAAGATCA ATTTGGCATT TCAGGTGGGG CCAGAGGTGA GAAAGGACAG 
AAGGGAGAGC CAGCCATCAT TGAACCCGGA ATGCTGATCG AAGGACCACC AGGCCCAGAA 
GGTCCAGCTG GTTATCCCGG TCCTCCTGGT ACCTCAGGTC CTCCTGGTCC AGCTGGAGAT 
CCTGGAGAGA GGGGCCCCCC TGGTCGTGCA GGTCTTCCTG GAGCTGATGG CTTGCCCGGA 
CCACCAGGAA CCATGCTGAT GTTGCCCTTC CGCTTCTCTG GTGGTGGAGA TGGTGGATCT 
AAAGGACCCC AAGTGTCTGC CCAGGAATCA CAAGCCCAAG CTATTCTCCA ACAAGCAAGA 
CTGGCGTTGA GAGGGCCATC TGGTCCGATG GGTCTTACTG GAAGACCAGG CCCATTGGGT 
TCTCCAGGTC CTTCAGGATT AAAGGGAGAG TCAGGAGATT TGGGACCTCA GGGGCCACGT 
GGGCCACAAG GTTCTCCCGG TCCATTAGGA AAACCAGGAC GAAGGGGACG AGCTGGTAGT 
GATGGTGCTC GGGGAATGCC TGGACAAACA GGACCAAAGG GTGACCGAGG CTTTGACGGG 
TTGGGCGGTT TGCCAGGAGA AAAGGGACAT CGTGGTGAGC CTGGTCCACA AGGTCCACCA 
GGGCCACATG GAGAAGACGG AGAAAGGGGT GATGATGGAG ACGTTGGACC TAGAGGGTTA 
CCAGGTGAAC CTGGACCCCG AGGGTTGCTT GGACCTAAAG GACCTCCTGG CCCACCGGGA 
ATCACCGGTG TTCCTGGAAT GGACGGTCAA GTTGGACCCA AAGGTAGTGT GGGACCTCAA 
GGTGAGCCAG GACCACCTGG GCAGCAAGGA AACCCAGGAG CACAGGGTCT TCCAGGTCCT 
CAGGGAGCTA TTGGACCACC AGGAGAGAAG GGACCACTGG GAAAATCAGG TCTTCCCGGA 
ATGCCCGGAG CTGATGGACC TCCCGGTCAC CCCGGGAAAG AAGGACCCTC AGGAGAGAAA 
GGAACCCTGG GTCCTGCAGG GCCACAGGGA CCAATTGGAT ACCCAGGACC ACGTGGTGTT 
AAGGGAGCTG ATGGAATTCG TGGTCTAAAG GGAACAAAAG GAGAAAAGGG TGAAGATGGA 
TTCCCTGGAT TTAAGGGTGA TATGGGCACC AAAGGAGACA GAGGTGAAAT CGGTCCTCCT 
GGTCCTAGAG GTGAAGATGG TCCTGAAGGT CCAAAGGGTC GAGGTGGTCC ATCTGGAGAA 
ATAGGTCCAC TTGGTCCTGG CGGTGAAAAG GGAAAGCTTG GTGTTCCTGG TCTACCAGGT 
TATCCAGGAA GACAAGGTCC AAAGGGTTCT ATCGGATTCC CAGGATTCGC AGGAGCAAAC 
GGAGAGAAAG GAACAAGGGG TACCCCTGGC AAACCGGGTC CAAGGGGGCA ACGAGGTCCA 
ACGGGTCCAC GTGGTGAAAG AGGTCCAAGG GGAGCTACAG GCAAGCCAGG CCCCAAGGGT 
AACTCCGGAG GGGATGGCCC AGCAGGTCCA CCTGGTGAAA GGGGACCACC TGGACCTCAA 
GGACCCACTG GATTTGCAGG ACCGAAGGGC CCCCCTGGTC CACCAGGGAA AGATGGACTT 
CCCGGCCACC CTGGACAGAG AGGAGAGACT GGATTCCAAG GAAAGACTGG CCCTCCTGGT 
CCCCCTGGCG TTGTCGGTCC TCAGGGTCCA ACTGGAGAGA CTGGCCCAAT GGGTGAAAGA 
GGACATCCTG GTCCTCCAGG TCCTCCTGGT GAGCAAGGTT TACCTGGTGT GGCTGGAAAA 
GAAGGATCCA AGGGAGACCC CGGTCCTGCT GGTTTGCCTG GAAAAGATGG ACCTCCAGGT 
CTAAGAGGTT TTACTGGAGA AAGAGGTCTC CCCGGTCCGA TTGGTGTTCC TGGACTGAAA 
GGCAATGAGG GTCCTCCTGG TCCACCTGGC CCAGCTGGTT CCCCCGGTGA AAGAGGAGCT 
GCGGGTCCAG CCGGACCTAT TGGACTGCCT GGAAGACCTG GCCCTCAGGG GCCACCAGGA 
CCTGCCGGCG AGAAGGGTAC ACCAGGTGAG AAAGGTCCCC AGGGACCTGC TGGACGTGAT 
GGTATCCAAG GCCCCGTTGG ACTTCCAGGC CCAGCTGGAC CTGTAGGGCC ACCTGGTGAA 
GATGGTGATA AGGGTGAAAT CGGAGAGCCT GGGCAAAAAG GAAACAGAGG AGATAAAGGA 
GAGCAGGGTC CCCCAGGTCC ACCTGGCCCA CAAGGTCCCA TTGGTCAGCC TGGCCCATCA 
GGATCTGATG GAGAGCCAGG CCCCAGGGGT CAACAAGGTC TTTTTGGACA AAAAGGTGAT 
GAAGGACCTA GAGGTTTTGC TGGACCACCA GGACCTGTTG GACTGCAGGG TTTACCTGGA 
CCACCTGGTG AGAAGGGAGA AACCGGAGAC GTTGGTCAGA TGGGTCCTCC TGGTCCCCCT 
GGTCCTAGAG GTCCTTCTGG CTCCCCTGGA GCTGATGGCC CACAAGGTCC TCCTGGAGGA 
ATTGGCAACC CTGGTCCTGT AGGAGAGAAG GGTGACCCTG GAGAGTCTGG GGAACCTGGT 
CTTCCTGGTG AACTTGGACC ACCGGGCCCT AAAGGTGAAA GAGGTGAAAA GGGAGAAGCG 
GGTCTTCAAG GAACTTCTGG CCCTCCAGGT CCTAAAGGTC CTCCAGGTGA TGATGGTCCA 
AAAGGTAGCC CTGGCCCAGT TGGATTTCCT GGTGATCCTG GTCCTCCTGG AGAATCTGGT 
CCTGCAGGTC AAGATGGTCC ACCTGGTGAT AAGGGAGAGG ACGGTGAATC TGGACAGCCA 
GGGTCACCAG GTCCTACTGG AGAGCCTGGG CCTTCAGGAC CACCTGGAAA GAGGGGTCCT 
CCTGGTCCAA CAGGTCCTGA AGGACGTCAA GGAGAAAAAG GAGCAAAGGG TGAATCAGGT 
TTGGAAGGAC CTCCTGGTAA GACTGGTCCT ATTGGTCCCC AAGGACCTCC CGGAAAACCT 
GGACCTGAAG GCCTTCGAGG AATTCCAGGC CCTGTTGGTG AACAAGGTCT CCCTGGTTCA 
CCTGGACCTG ATGGCCCTCC CGGACCTATG GGTCCACCAG GTATACCAGG TCTGAAAGGA 
GACTCAGGTC CAAAAGGAGA AAAGGGTCAT CCAGGCTTGA TAGGTCTCAT TGGACCTCCT 
GGTGAACAGG GAGAAAAGGG AGACCGTGGT TTGCCAGGCC CCCAGGGACC ACATGGTGCT 
AAAGGAGAAC AGGGTATTCT TGGTCCATCT GGTCCTGTTG GTCCACCTGG TCCTCCAGGC 
TTACCGGGTG GTCCTGGTCC AAAAGGTGCT AAAGGTTCAT CGGGTCCAAC TGGTCCAAAG 
GGTGAATCTG GAATCCCTGG TCCACCAGGT CCTCCAGGAC CTCCAGGCGA GGTGATCCAG 
CCTCTGCCTA TCCATTCCTC AAAGAGGACA AGACGTAGCA TTGATGCAAG TCAAGTAGTG 
GACGATGGAA ATGCAGACAA CTACATGGAC TATGCCGATG GAATGGAGGA AATATTTGGT 
TCTCTCAACT CCCTAAAACA GGAAATTGAA CAAATGAAAC ACCCACTGGG CAATCAGCAG 
AACCCAGCAA GGACTTGCAA GGACCTGCTT CTGTGCCATC CTGAACTCCC AGATGGGGAA 
TATTGGATTG ACCCAAATCA AGGATGCTCT AGAGACTCGT TCAAGGTTTT CTGTAATTTC 
ACAGCCGGTG GAGAATCTTG TATCTTCCCC GACAAGAAGT CTGAAGGAAA TAAACTGGCT 
CGTTGGCCCA AAGAGCTGCC TGGTACATGG TATAGTCATT ACAAGCGGGG TTCCATGTTG 
TCCTACGTTG ATTCAAATGG CAACCCCATT GGAGTGGTGC AGATGACCTT CATGAAGCTT 
CTGAGTGCTT CGGTGAAACA GAATATCACC TACAACTGCT ACCAGTCCGT GGCATGGCAC 
GACGCCACCA CCAACAGCTA CGACAAGGCC ATTCGCTTCC TGGGGTCCAA CGACGAAGAA 
ATGTCCTACG ACAACAATCC TTCCATTCGA GCCATTATGG ATGGATGTGC GTCAAAGAAA 
GGTTATCAGA AGACAGTTCT GGAACTCGAC ACGTCCAAAG TGGAACAGGC CCCCATTGTG 
GATATTATGT TCAACGACTT CGGAGATCCA GTACAAAAAT TCGGCTTTGA GGTGGGACCA 
GTGTGTTTCA TGGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824629.1
CDS	343..5940
Translation	MSMDSHTRWKRRNWTTEWPLLVALALSICSRFSAAEPADLLKALEFHNLPDGIKKTTGFCAYRKSSDGPDVAFRVSKEAQLSAPTNQLFSDVAFPEDFSLLATVKPKKGSHSYLLSIYNEQGTLQIGLTLGWEPMFVYEDHQGHPPPEDYPIFRNINLSDGKWHRVAFSVHKKTITLFLDCVKKATLPLKRSDGPIIDTNGIVVFGTRILDEQVFEGDIQQLMIVPDARAAHDYCEHYTPDCDTAIPDAPQSQDPNQDEYYPEAAEAEGEGYYYEYPYYEDEKTETATTKPTPEKAEVLRPETEIEEEETPPPTEAPAIPVTDAPRFETREESRVESRVESRVDNPTTDDYNYETVQEEYYTASPYEDVNYSEGEETGIETGTTGEGLIEGEGEILTKTVITSSSSNGSSVISSGSSKKEGSTDGDFTEVTIQEYDQGKDTYYDIYDDKLLTDTSDGIGGQPAEQDQFGISGGARGEKGQKGEPAIIEPGMLIEGPPGPEGPAGYPGPPGTSGPPGPAGDPGERGPPGRAGLPGADGLPGPPGTMLMLPFRFSGGGDGGSKGPQVSAQESQAQAILQQARLALRGPSGPMGLTGRPGPLGSPGPSGLKGESGDLGPQGPRGPQGSPGPLGKPGRRGRAGSDGARGMPGQTGPKGDRGFDGLGGLPGEKGHRGEPGPQGPPGPHGEDGERGDDGDVGPRGLPGEPGPRGLLGPKGPPGPPGITGVPGMDGQVGPKGSVGPQGEPGPPGQQGNPGAQGLPGPQGAIGPPGEKGPLGKSGLPGMPGADGPPGHPGKEGPSGEKGTLGPAGPQGPIGYPGPRGVKGADGIRGLKGTKGEKGEDGFPGFKGDMGTKGDRGEIGPPGPRGEDGPEGPKGRGGPSGEIGPLGPGGEKGKLGVPGLPGYPGRQGPKGSIGFPGFAGANGEKGTRGTPGKPGPRGQRGPTGPRGERGPRGATGKPGPKGNSGGDGPAGPPGERGPPGPQGPTGFAGPKGPPGPPGKDGLPGHPGQRGETGFQGKTGPPGPPGVVGPQGPTGETGPMGERGHPGPPGPPGEQGLPGVAGKEGSKGDPGPAGLPGKDGPPGLRGFTGERGLPGPIGVPGLKGNEGPPGPPGPAGSPGERGAAGPAGPIGLPGRPGPQGPPGPAGEKGTPGEKGPQGPAGRDGIQGPVGLPGPAGPVGPPGEDGDKGEIGEPGQKGNRGDKGEQGPPGPPGPQGPIGQPGPSGSDGEPGPRGQQGLFGQKGDEGPRGFAGPPGPVGLQGLPGPPGEKGETGDVGQMGPPGPPGPRGPSGSPGADGPQGPPGGIGNPGPVGEKGDPGESGEPGLPGELGPPGPKGERGEKGEAGLQGTSGPPGPKGPPGDDGPKGSPGPVGFPGDPGPPGESGPAGQDGPPGDKGEDGESGQPGSPGPTGEPGPSGPPGKRGPPGPTGPEGRQGEKGAKGESGLEGPPGKTGPIGPQGPPGKPGPEGLRGIPGPVGEQGLPGSPGPDGPPGPMGPPGIPGLKGDSGPKGEKGHPGLIGLIGPPGEQGEKGDRGLPGPQGPHGAKGEQGILGPSGPVGPPGPPGLPGGPGPKGAKGSSGPTGPKGESGIPGPPGPPGPPGEVIQPLPIHSSKRTRRSIDASQVVDDGNADNYMDYADGMEEIFGSLNSLKQEIEQMKHPLGNQQNPARTCKDLLLCHPELPDGEYWIDPNQGCSRDSFKVFCNFTAGGESCIFPDKKSEGNKLARWPKELPGTWYSHYKRGSMLSYVDSNGNPIGVVQMTFMKLLSASVKQNITYNCYQSVAWHDATTNSYDKAIRFLGSNDEEMSYDNNPSIRAIMDGCASKKGYQKTVLELDTSKVEQAPIVDIMFNDFGDPVQKFGFEVGPVCFMG

Target ORF information:

RefSeq Version	XM_012969175.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen, type V, alpha 1 (col5a1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012969175.3

ORF Insert Sequence:

ATGAGTATGG ATTCCCACAC ACGATGGAAA AGACGGAATT GGACGACAGA GTGGCCCCTC 
CTTGTAGCTT TAGCCTTGTC TATATGTTCC CGGTTCAGCG CAGCTGAACC GGCGGACCTG 
TTGAAGGCCT TAGAATTTCA CAATTTACCA GACGGTATCA AAAAAACGAC GGGCTTCTGC 
GCCTACCGCA AATCTTCAGA TGGACCAGAC GTGGCATTTA GAGTGTCCAA AGAGGCCCAG 
CTTAGCGCTC CGACCAATCA ATTGTTCTCC GATGTCGCAT TCCCAGAAGA TTTCTCCTTG 
TTGGCAACTG TGAAACCCAA AAAAGGAAGC CATTCCTACC TGCTCTCCAT CTACAATGAG 
CAAGGAACCC TGCAGATCGG CCTGACGCTG GGCTGGGAAC CGATGTTCGT CTATGAAGAC 
CACCAGGGAC ACCCACCCCC CGAGGATTAC CCCATCTTTA GAAACATTAA TCTGTCAGAT 
GGGAAGTGGC ACCGGGTAGC TTTCAGCGTG CACAAGAAGA CGATCACCCT GTTCCTGGAC 
TGCGTCAAGA AGGCCACCCT GCCCTTGAAA AGAAGCGACG GCCCCATTAT AGACACGAAC 
GGCATCGTCG TGTTCGGCAC CAGGATCCTG GACGAGCAAG TCTTTGAGGG AGACATTCAA 
CAGCTGATGA TCGTGCCCGA CGCCCGTGCT GCACATGATT ATTGTGAACA CTACACCCCC 
GACTGTGACA CCGCCATTCC AGATGCACCG CAGTCTCAGG ATCCCAACCA AGATGAATAT 
TACCCAGAAG CTGCTGAAGC CGAAGGCGAG GGTTATTACT ATGAATATCC TTACTACGAA 
GATGAGAAGA CAGAAACTGC AACTACCAAG CCCACGCCTG AGAAAGCCGA GGTACTAAGG 
CCCGAGACAG AAATCGAAGA GGAGGAAACA CCCCCACCAA CTGAAGCCCC TGCTATTCCC 
GTCACTGATG CTCCACGATT TGAGACCAGG GAAGAGAGCA GGGTAGAGAG CAGGGTAGAG 
AGCAGAGTGG ATAACCCCAC CACCGATGAC TACAACTATG AAACCGTACA GGAAGAATAT 
TACACCGCCT CTCCTTATGA AGATGTCAAC TACTCTGAAG GAGAGGAAAC AGGAATTGAG 
ACTGGGACTA CTGGTGAAGG CCTCATCGAG GGAGAAGGAG AAATTTTGAC CAAAACTGTC 
ATCACAAGCA GCTCATCCAA TGGCTCATCG GTCATCAGCA GTGGAAGTTC CAAAAAAGAA 
GGCAGTACCG ACGGAGATTT CACAGAAGTG ACCATACAGG AGTACGACCA AGGGAAAGAT 
ACTTACTATG ACATCTACGA TGATAAGCTT CTCACTGACA CTTCAGACGG AATTGGTGGG 
CAGCCAGCTG AACAAGATCA ATTTGGCATT TCAGGTGGGG CCAGAGGTGA GAAAGGACAG 
AAGGGAGAGC CAGCCATCAT TGAACCCGGA ATGCTGATCG AAGGACCACC AGGCCCAGAA 
GGTCCAGCTG GTTATCCCGG TCCTCCTGGT ACCTCAGGTC CTCCTGGTCC AGCTGGAGAT 
CCTGGAGAGA GGGGCCCCCC TGGTCGTGCA GGTCTTCCTG GAGCTGATGG CTTGCCCGGA 
CCACCAGGAA CCATGCTGAT GTTGCCCTTC CGCTTCTCTG GTGGTGGAGA TGGTGGATCT 
AAAGGACCCC AAGTGTCTGC CCAGGAATCA CAAGCCCAAG CTATTCTCCA ACAAGCAAGA 
CTGGCGTTGA GAGGGCCATC TGGTCCGATG GGTCTTACTG GAAGACCAGG CCCATTGGGT 
TCTCCAGGTC CTTCAGGATT AAAGGGAGAG TCAGGAGATT TGGGACCTCA GGGGCCACGT 
GGGCCACAAG GTTCTCCCGG TCCATTAGGA AAACCAGGAC GAAGGGGACG AGCTGGTAGT 
GATGGTGCTC GGGGAATGCC TGGACAAACA GGACCAAAGG GTGACCGAGG CTTTGACGGG 
TTGGGCGGTT TGCCAGGAGA AAAGGGACAT CGTGGTGAGC CTGGTCCACA AGGTCCACCA 
GGGCCACATG GAGAAGACGG AGAAAGGGGT GATGATGGAG ACGTTGGACC TAGAGGGTTA 
CCAGGTGAAC CTGGACCCCG AGGGTTGCTT GGACCTAAAG GACCTCCTGG CCCACCGGGA 
ATCACCGGTG TTCCTGGAAT GGACGGTCAA GTTGGACCCA AAGGTAGTGT GGGACCTCAA 
GGTGAGCCAG GACCACCTGG GCAGCAAGGA AACCCAGGAG CACAGGGTCT TCCAGGTCCT 
CAGGGAGCTA TTGGACCACC AGGAGAGAAG GGACCACTGG GAAAATCAGG TCTTCCCGGA 
ATGCCCGGAG CTGATGGACC TCCCGGTCAC CCCGGGAAAG AAGGACCCTC AGGAGAGAAA 
GGAACCCTGG GTCCTGCAGG GCCACAGGGA CCAATTGGAT ACCCAGGACC ACGTGGTGTT 
AAGGGAGCTG ATGGAATTCG TGGTCTAAAG GGAACAAAAG GAGAAAAGGG TGAAGATGGA 
TTCCCTGGAT TTAAGGGTGA TATGGGCACC AAAGGAGACA GAGGTGAAAT CGGTCCTCCT 
GGTCCTAGAG GTGAAGATGG TCCTGAAGGT CCAAAGGGTC GAGGTGGTCC ATCTGGAGAA 
ATAGGTCCAC TTGGTCCTGG CGGTGAAAAG GGAAAGCTTG GTGTTCCTGG TCTACCAGGT 
TATCCAGGAA GACAAGGTCC AAAGGGTTCT ATCGGATTCC CAGGATTCGC AGGAGCAAAC 
GGAGAGAAAG GAACAAGGGG TACCCCTGGC AAACCGGGTC CAAGGGGGCA ACGAGGTCCA 
ACGGGTCCAC GTGGTGAAAG AGGTCCAAGG GGAGCTACAG GCAAGCCAGG CCCCAAGGGT 
AACTCCGGAG GGGATGGCCC AGCAGGTCCA CCTGGTGAAA GGGGACCACC TGGACCTCAA 
GGACCCACTG GATTTGCAGG ACCGAAGGGC CCCCCTGGTC CACCAGGGAA AGATGGACTT 
CCCGGCCACC CTGGACAGAG AGGAGAGACT GGATTCCAAG GAAAGACTGG CCCTCCTGGT 
CCCCCTGGCG TTGTCGGTCC TCAGGGTCCA ACTGGAGAGA CTGGCCCAAT GGGTGAAAGA 
GGACATCCTG GTCCTCCAGG TCCTCCTGGT GAGCAAGGTT TACCTGGTGT GGCTGGAAAA 
GAAGGATCCA AGGGAGACCC CGGTCCTGCT GGTTTGCCTG GAAAAGATGG ACCTCCAGGT 
CTAAGAGGTT TTACTGGAGA AAGAGGTCTC CCCGGTCCGA TTGGTGTTCC TGGACTGAAA 
GGCAATGAGG GTCCTCCTGG TCCACCTGGC CCAGCTGGTT CCCCCGGTGA AAGAGGAGCT 
GCGGGTCCAG CCGGACCTAT TGGACTGCCT GGAAGACCTG GCCCTCAGGG GCCACCAGGA 
CCTGCCGGCG AGAAGGGTAC ACCAGGTGAG AAAGGTCCCC AGGGACCTGC TGGACGTGAT 
GGTATCCAAG GCCCCGTTGG ACTTCCAGGC CCAGCTGGAC CTGTAGGGCC ACCTGGTGAA 
GATGGTGATA AGGGTGAAAT CGGAGAGCCT GGGCAAAAAG GAAACAGAGG AGATAAAGGA 
GAGCAGGGTC CCCCAGGTCC ACCTGGCCCA CAAGGTCCCA TTGGTCAGCC TGGCCCATCA 
GGATCTGATG GAGAGCCAGG CCCCAGGGGT CAACAAGGTC TTTTTGGACA AAAAGGTGAT 
GAAGGACCTA GAGGTTTTGC TGGACCACCA GGACCTGTTG GACTGCAGGG TTTACCTGGA 
CCACCTGGTG AGAAGGGAGA AACCGGAGAC GTTGGTCAGA TGGGTCCTCC TGGTCCCCCT 
GGTCCTAGAG GTCCTTCTGG CTCCCCTGGA GCTGATGGCC CACAAGGTCC TCCTGGAGGA 
ATTGGCAACC CTGGTCCTGT AGGAGAGAAG GGTGACCCTG GAGAGTCTGG GGAACCTGGT 
CTTCCTGGTG AACTTGGACC ACCGGGCCCT AAAGGTGAAA GAGGTGAAAA GGGAGAAGCG 
GGTCTTCAAG GAACTTCTGG CCCTCCAGGT CCTAAAGGTC CTCCAGGTGA TGATGGTCCA 
AAAGGTAGCC CTGGCCCAGT TGGATTTCCT GGTGATCCTG GTCCTCCTGG AGAATCTGGT 
CCTGCAGGTC AAGATGGTCC ACCTGGTGAT AAGGGAGAGG ACGGTGAATC TGGACAGCCA 
GGGTCACCAG GTCCTACTGG AGAGCCTGGG CCTTCAGGAC CACCTGGAAA GAGGGGTCCT 
CCTGGTCCAA CAGGTCCTGA AGGACGTCAA GGAGAAAAAG GAGCAAAGGG TGAATCAGGT 
TTGGAAGGAC CTCCTGGTAA GACTGGTCCT ATTGGTCCCC AAGGACCTCC CGGAAAACCT 
GGACCTGAAG GCCTTCGAGG AATTCCAGGC CCTGTTGGTG AACAAGGTCT CCCTGGTTCA 
CCTGGACCTG ATGGCCCTCC CGGACCTATG GGTCCACCAG GTATACCAGG TCTGAAAGGA 
GACTCAGGTC CAAAAGGAGA AAAGGGTCAT CCAGGCTTGA TAGGTCTCAT TGGACCTCCT 
GGTGAACAGG GAGAAAAGGG AGACCGTGGT TTGCCAGGCC CCCAGGGACC ACATGGTGCT 
AAAGGAGAAC AGGGTATTCT TGGTCCATCT GGTCCTGTTG GTCCACCTGG TCCTCCAGGC 
TTACCGGGTG GTCCTGGTCC AAAAGGTGCT AAAGGTTCAT CGGGTCCAAC TGGTCCAAAG 
GGTGAATCTG GAATCCCTGG TCCACCAGGT CCTCCAGGAC CTCCAGGCGA GGTGATCCAG 
CCTCTGCCTA TCCATTCCTC AAAGAGGACA AGACGTAGCA TTGATGCAAG TCAAGTAGTG 
GACGATGGAA ATGCAGACAA CTACATGGAC TATGCCGATG GAATGGAGGA AATATTTGGT 
TCTCTCAACT CCCTAAAACA GGAAATTGAA CAAATGAAAC ACCCACTGGG CAATCAGCAG 
AACCCAGCAA GGACTTGCAA GGACCTGCTT CTGTGCCATC CTGAACTCCC AGATGGGGAA 
TATTGGATTG ACCCAAATCA AGGATGCTCT AGAGACTCGT TCAAGGTTTT CTGTAATTTC 
ACAGCCGGTG GAGAATCTTG TATCTTCCCC GACAAGAAGT CTGAAGGAAA TAAACTGGCT 
CGTTGGCCCA AAGAGCTGCC TGGTACATGG TATAGTCATT ACAAGCGGGG TTCCATGTTG 
TCCTACGTTG ATTCAAATGG CAACCCCATT GGAGTGGTGC AGATGACCTT CATGAAGCTT 
CTGAGTGCTT CGGTGAAACA GAATATCACC TACAACTGCT ACCAGTCCGT GGCATGGCAC 
GACGCCACCA CCAACAGCTA CGACAAGGCC ATTCGCTTCC TGGGGTCCAA CGACGAAGAA 
ATGTCCTACG ACAACAATCC TTCCATTCGA GCCATTATGG ATGGATGTGC GTCAAAGAAA 
GGTTATCAGA AGACAGTTCT GGAACTCGAC ACGTCCAAAG TGGAACAGGC CCCCATTGTG 
GATATTATGT TCAACGACTT CGGAGATCCA GTACAAAAAT TCGGCTTTGA GGTGGGACCA 
GTGTGTTTCA TGGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col5a1 ( XM_012969176.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824630.1 Xenopus tropicalis collagen, type V, alpha 1 (col5a1), transcript variant X3, mRNA.

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CloneID	OXa30188
Clone ID Related Accession (Same CDS sequence)	XM_012969176.2 , XM_012969176.3
Accession Version	XM_012969176.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-1(V) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012969176.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTATGG ATTCCCACAC ACGATGGAAA AGACGGAATT GGACGACAGA GTGGCCCCTC 
CTTGTAGCTT TAGCCTTGTC TATATGTTCC CGGTTCAGCG CAGCTGAACC GGCGGACCTG 
TTGAAGGCCT TAGAATTTCA CAATTTACCA GACGGTATCA AAAAAACGAC GGGCTTCTGC 
GCCTACCGCA AATCTTCAGA TGGACCAGAC GTGGCATTTA GAGTGTCCAA AGAGGCCCAG 
CTTAGCGCTC CGACCAATCA ATTGTTCTCC GATGTCGCAT TCCCAGAAGA TTTCTCCTTG 
TTGGCAACTG TGAAACCCAA AAAAGGAAGC CATTCCTACC TGCTCTCCAT CTACAATGAG 
CAAGGAACCC TGCAGATCGG CCTGACGCTG GGCTGGGAAC CGATGTTCGT CTATGAAGAC 
CACCAGGGAC ACCCACCCCC CGAGGATTAC CCCATCTTTA GAAACATTAA TCTGTCAGAT 
GGGAAGTGGC ACCGGGTAGC TTTCAGCGTG CACAAGAAGA CGATCACCCT GTTCCTGGAC 
TGCGTCAAGA AGGCCACCCT GCCCTTGAAA AGAAGCGACG GCCCCATTAT AGACACGAAC 
GGCATCGTCG TGTTCGGCAC CAGGATCCTG GACGAGCAAG TCTTTGAGGG AGACATTCAA 
CAGCTGATGA TCGTGCCCGA CGCCCGTGCT GCACATGATT ATTGTGAACA CTACACCCCC 
GACTGTGACA CCGCCATTCC AGATGCACCG CAGTCTCAGG ATCCCAACCA AGATGAATAT 
TACCCAGAAG CTGCTGAAGC CGAAGGCGAG GGTTATTACT ATGAATATCC TTACTACGAA 
GATGAGAAGA CAGAAACTGC AACTACCAAG CCCACGCCTG AGAAAGCCGA GGTACTAAGG 
CCCGAGACAG AAATCGAAGA GGAGGAAACA CCCCCACCAA CTGAAGCCCC TGCTATTCCC 
GTCACTGATG CTCCACGATT TGAGACCAGG GAAGAGAGCA GGGTAGAGAG CAGGGTAGAG 
AGCAGAGTGG ATAACCCCAC CACCGATGAC TACAACTATG AAACCGTACA GGAAGAATAT 
TACACCGCCT CTCCTTATGA AGATGTCAAC TACTCTGAAG GAGAGGAAAC AGGAATTGAG 
ACTGGGACTA CTGGTGAAGG CCTCATCGAG GGAGAAGGAG AAATTTTGAC CAAAACTGTC 
ATCACAAGCA GCTCATCCAA TGGCTCATCG GTCATCAGCA GTGGAAGTTC CAAAAAAGAA 
GGCAGTACCG ACGGAGATTT CACAGAAGTG ACCATACAGG AGTACGACCA AGGGAAAGAT 
ACTTACTATG ACATCTACGA TGATAAGCTT CTCACTGACA CTTCAGACGG AATTGGTGGG 
CAGCCAGCTG AACAAGATCA ATTTGGCATT TCAGGTGGGG CCAGAGGTGA GAAAGGACAG 
AAGGGAGAGC CAGCCATCAT TGAACCCGGA ATGCTGATCG AAGGACCACC AGGCCCAGAA 
GGTCCAGCTG GTTATCCCGG TCCTCCTGGT ACCTCAGGTC CTCCTGGTCC AGCTGGAGAT 
CCTGGAGAGA GGGGCCCCCC TGGTCGTGCA GGTCTTCCTG GAGCTGATGG CTTGCCCGGA 
CCACCAGGAA CCATGCTGAT GTTGCCCTTC CGCTTCTCTG GTGGTGGAGA TGGTGGATCT 
AAAGGACCCC AAGTGTCTGC CCAGGAATCA CAAGCCCAAG CTATTCTCCA ACAAGCAAGA 
CTGGCGTTGA GAGGGCCATC TGGTCCGATG GGTCTTACTG GAAGACCAGG CCCATTGGGT 
TCTCCAGGTC CTTCAGGATT AAAGGGAGAG TCAGGAGATT TGGGACCTCA GGGGCCACGT 
GGGCCACAAG GTTCTCCCGG TCCATTAGGA AAACCAGGAC GAAGGGGACG AGCTGGTAGT 
GATGGTGCTC GGGGAATGCC TGGACAAACA GGACCAAAGG GTGACCGAGG CTTTGACGGG 
TTGGGCGGTT TGCCAGGAGA AAAGGGACAT CGTGGTGAGC CTGGTCCACA AGGTCCACCA 
GGGCCACATG GAGAAGACGG AGAAAGGGGT GATGATGGAG ACGTTGGACC TAGAGGGTTA 
CCAGGTGAAC CTGGACCCCG AGGGTTGCTT GGACCTAAAG GACCTCCTGG CCCACCGGGA 
ATCACCGGTG TTCCTGGAAT GGACGGTCAA GTTGGACCCA AAGGTAGTGT GGGACCTCAA 
GGTGAGCCAG GACCACCTGG GCAGCAAGGA AACCCAGGAG CACAGGGTCT TCCAGGTCCT 
CAGGGAGCTA TTGGACCACC AGGAGAGAAG GGACCACTGG GAAAATCAGG TCTTCCCGGA 
ATGCCCGGAG CTGATGGACC TCCCGGTCAC CCCGGGAAAG AAGGACCCTC AGGAGAGAAA 
GGAACCCTGG GTCCTGCAGG GCCACAGGGA CCAATTGGAT ACCCAGGACC ACGTGGTGTT 
AAGGGAGCTG ATGGAATTCG TGGTCTAAAG GGAACAAAAG GAGAAAAGGG TGAAGATGGA 
TTCCCTGGAT TTAAGGGTGA TATGGGCACC AAAGGAGACA GAGGTGAAAT CGGTCCTCCT 
GGTCCTAGAG GTGAAGATGG TCCTGAAGGT CCAAAGGGTC GAGGTGGTCC ATCTGGAGAA 
ATAGGTCCAC TTGGTCCTGG CGGTGAAAAG GGAAAGCTTG GTGTTCCTGG TCTACCAGGT 
TATCCAGGAA GACAAGGTCC AAAGGGTTCT ATCGGATTCC CAGGATTCGC AGGAGCAAAC 
GGAGAGAAAG GAACAAGGGG TACCCCTGGC AAACCGGGTC CAAGGGGGCA ACGAGGTCCA 
ACGGGTCCAC GTGGTGAAAG AGGTCCAAGG GGAGCTACAG GCAAGCCAGG CCCCAAGGGT 
AACTCCGGAG GGGATGGCCC AGCAGGTCCA CCTGGTGAAA GGGGACCACC TGGACCTCAA 
GGACCCACTG GATTTGCAGG ACCGAAGGGC CCCCCTGGTC CACCAGGGAA AGATGGACTT 
CCCGGCCACC CTGGACAGAG AGGAGAGACT GGATTCCAAG GAAAGACTGG CCCTCCTGGT 
CCCCCTGGCG TTGTCGGTCC TCAGGGTCCA ACTGGAGAGA CTGGCCCAAT GGGTGAAAGA 
GGACATCCTG GTCCTCCAGG TCCTCCTGGT GAGCAAGGTT TACCTGGTGT GGCTGGAAAA 
GAAGGATCCA AGGGAGACCC CGGTCCTGCT GGTTTGCCTG GAAAAGATGG ACCTCCAGGT 
CTAAGAGGTT TTACTGGAGA AAGAGGTCTC CCCGGTCCGA TTGGTGTTCC TGGACTGAAA 
GGCAATGAGG GTCCTCCTGG TCCACCTGGC CCAGCTGGTT CCCCCGGTGA AAGAGGAGCT 
GCGGGTCCAG CCGGACCTAT TGGACTGCCT GGAAGACCTG GCCCTCAGGG GCCACCAGGA 
CCTGCCGGCG AGAAGGGTAC ACCAGGTGAG AAAGGTCCCC AGGGACCTGC TGGACGTGAT 
GGTATCCAAG GCCCCGTTGG ACTTCCAGGC CCAGCTGGAC CTGTAGGGCC ACCTGGTGAA 
GATGGTGATA AGGGTGAAAT CGGAGAGCCT GGGCAAAAAG GAAACAGAGG AGATAAAGGA 
GAGCAGGGTC CCCCAGGTCC ACCTGGCCCA CAAGGTCCCA TTGGTCAGCC TGGCCCATCA 
GGATCTGATG GAGAGCCAGG CCCCAGGGGT CAACAAGGTC TTTTTGGACA AAAAGGTGAT 
GAAGGACCTA GAGGTTTTGC TGGACCACCA GGACCTGTTG GACTGCAGGG TTTACCTGGA 
CCACCTGGTG AGAAGGGAGA AACCGGAGAC GTTGGTCAGA TGGGTCCTCC TGGTCCCCCT 
GGTCCTAGAG GTCCTTCTGG CTCCCCTGGA GCTGATGGCC CACAAGGTCC TCCTGGAGGA 
ATTGGCAACC CTGGTCCTGT AGGAGAGAAG GGTGACCCTG GAGAGTCTGG GGAACCTGGT 
CTTCCTGGTG AACTTGGACC ACCGGGCCCT AAAGGTGAAA GAGGTGAAAA GGGAGAAGCG 
GGTCTTCAAG GAACTTCTGG CCCTCCAGGT CCTAAAGGTC CTCCAGGTGA TGATGGTCCA 
AAAGGTAGCC CTGGCCCAGT TGGATTTCCT GGTGATCCTG GTCCTCCTGG AGAATCTGGT 
CCTGCAGGTC AAGATGGTCC ACCTGGTGAT AAGGGAGAGG ACGGTGAATC TGGACAGCCA 
GGGTCACCAG GTCCTACTGG AGAGCCTGGG CCTTCAGGAC CACCTGGAAA GAGGGGTCCT 
CCTGGTCCAA CAGGTCCTGA AGGACGTCAA GGAGAAAAAG GAGCAAAGGG TGAATCAGGT 
TTGGAAGGAC CTCCTGGTAA GACTGGTCCT ATTGGTCCCC AAGGACCTCC CGGAAAACCT 
GGACCTGAAG GCCTTCGAGG AATTCCAGGC CCTGTTGGTG AACAAGGTCT CCCTGGTTCA 
CCTGGACCTG ATGGCCCTCC CGGACCTATG GGTCCACCAG GTATACCAGG TCTGAAAGGA 
GACTCAGGTC CAAAAGGAGA AAAGGGTCAT CCAGGCTTGA TAGGTCTCAT TGGACCTCCT 
GGTGAAACTA AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824630.1
CDS	344..4918
Translation	MSMDSHTRWKRRNWTTEWPLLVALALSICSRFSAAEPADLLKALEFHNLPDGIKKTTGFCAYRKSSDGPDVAFRVSKEAQLSAPTNQLFSDVAFPEDFSLLATVKPKKGSHSYLLSIYNEQGTLQIGLTLGWEPMFVYEDHQGHPPPEDYPIFRNINLSDGKWHRVAFSVHKKTITLFLDCVKKATLPLKRSDGPIIDTNGIVVFGTRILDEQVFEGDIQQLMIVPDARAAHDYCEHYTPDCDTAIPDAPQSQDPNQDEYYPEAAEAEGEGYYYEYPYYEDEKTETATTKPTPEKAEVLRPETEIEEEETPPPTEAPAIPVTDAPRFETREESRVESRVESRVDNPTTDDYNYETVQEEYYTASPYEDVNYSEGEETGIETGTTGEGLIEGEGEILTKTVITSSSSNGSSVISSGSSKKEGSTDGDFTEVTIQEYDQGKDTYYDIYDDKLLTDTSDGIGGQPAEQDQFGISGGARGEKGQKGEPAIIEPGMLIEGPPGPEGPAGYPGPPGTSGPPGPAGDPGERGPPGRAGLPGADGLPGPPGTMLMLPFRFSGGGDGGSKGPQVSAQESQAQAILQQARLALRGPSGPMGLTGRPGPLGSPGPSGLKGESGDLGPQGPRGPQGSPGPLGKPGRRGRAGSDGARGMPGQTGPKGDRGFDGLGGLPGEKGHRGEPGPQGPPGPHGEDGERGDDGDVGPRGLPGEPGPRGLLGPKGPPGPPGITGVPGMDGQVGPKGSVGPQGEPGPPGQQGNPGAQGLPGPQGAIGPPGEKGPLGKSGLPGMPGADGPPGHPGKEGPSGEKGTLGPAGPQGPIGYPGPRGVKGADGIRGLKGTKGEKGEDGFPGFKGDMGTKGDRGEIGPPGPRGEDGPEGPKGRGGPSGEIGPLGPGGEKGKLGVPGLPGYPGRQGPKGSIGFPGFAGANGEKGTRGTPGKPGPRGQRGPTGPRGERGPRGATGKPGPKGNSGGDGPAGPPGERGPPGPQGPTGFAGPKGPPGPPGKDGLPGHPGQRGETGFQGKTGPPGPPGVVGPQGPTGETGPMGERGHPGPPGPPGEQGLPGVAGKEGSKGDPGPAGLPGKDGPPGLRGFTGERGLPGPIGVPGLKGNEGPPGPPGPAGSPGERGAAGPAGPIGLPGRPGPQGPPGPAGEKGTPGEKGPQGPAGRDGIQGPVGLPGPAGPVGPPGEDGDKGEIGEPGQKGNRGDKGEQGPPGPPGPQGPIGQPGPSGSDGEPGPRGQQGLFGQKGDEGPRGFAGPPGPVGLQGLPGPPGEKGETGDVGQMGPPGPPGPRGPSGSPGADGPQGPPGGIGNPGPVGEKGDPGESGEPGLPGELGPPGPKGERGEKGEAGLQGTSGPPGPKGPPGDDGPKGSPGPVGFPGDPGPPGESGPAGQDGPPGDKGEDGESGQPGSPGPTGEPGPSGPPGKRGPPGPTGPEGRQGEKGAKGESGLEGPPGKTGPIGPQGPPGKPGPEGLRGIPGPVGEQGLPGSPGPDGPPGPMGPPGIPGLKGDSGPKGEKGHPGLIGLIGPPGETK

Target ORF information:

RefSeq Version	XM_012969176.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen, type V, alpha 1 (col5a1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012969176.3

ORF Insert Sequence:

ATGAGTATGG ATTCCCACAC ACGATGGAAA AGACGGAATT GGACGACAGA GTGGCCCCTC 
CTTGTAGCTT TAGCCTTGTC TATATGTTCC CGGTTCAGCG CAGCTGAACC GGCGGACCTG 
TTGAAGGCCT TAGAATTTCA CAATTTACCA GACGGTATCA AAAAAACGAC GGGCTTCTGC 
GCCTACCGCA AATCTTCAGA TGGACCAGAC GTGGCATTTA GAGTGTCCAA AGAGGCCCAG 
CTTAGCGCTC CGACCAATCA ATTGTTCTCC GATGTCGCAT TCCCAGAAGA TTTCTCCTTG 
TTGGCAACTG TGAAACCCAA AAAAGGAAGC CATTCCTACC TGCTCTCCAT CTACAATGAG 
CAAGGAACCC TGCAGATCGG CCTGACGCTG GGCTGGGAAC CGATGTTCGT CTATGAAGAC 
CACCAGGGAC ACCCACCCCC CGAGGATTAC CCCATCTTTA GAAACATTAA TCTGTCAGAT 
GGGAAGTGGC ACCGGGTAGC TTTCAGCGTG CACAAGAAGA CGATCACCCT GTTCCTGGAC 
TGCGTCAAGA AGGCCACCCT GCCCTTGAAA AGAAGCGACG GCCCCATTAT AGACACGAAC 
GGCATCGTCG TGTTCGGCAC CAGGATCCTG GACGAGCAAG TCTTTGAGGG AGACATTCAA 
CAGCTGATGA TCGTGCCCGA CGCCCGTGCT GCACATGATT ATTGTGAACA CTACACCCCC 
GACTGTGACA CCGCCATTCC AGATGCACCG CAGTCTCAGG ATCCCAACCA AGATGAATAT 
TACCCAGAAG CTGCTGAAGC CGAAGGCGAG GGTTATTACT ATGAATATCC TTACTACGAA 
GATGAGAAGA CAGAAACTGC AACTACCAAG CCCACGCCTG AGAAAGCCGA GGTACTAAGG 
CCCGAGACAG AAATCGAAGA GGAGGAAACA CCCCCACCAA CTGAAGCCCC TGCTATTCCC 
GTCACTGATG CTCCACGATT TGAGACCAGG GAAGAGAGCA GGGTAGAGAG CAGGGTAGAG 
AGCAGAGTGG ATAACCCCAC CACCGATGAC TACAACTATG AAACCGTACA GGAAGAATAT 
TACACCGCCT CTCCTTATGA AGATGTCAAC TACTCTGAAG GAGAGGAAAC AGGAATTGAG 
ACTGGGACTA CTGGTGAAGG CCTCATCGAG GGAGAAGGAG AAATTTTGAC CAAAACTGTC 
ATCACAAGCA GCTCATCCAA TGGCTCATCG GTCATCAGCA GTGGAAGTTC CAAAAAAGAA 
GGCAGTACCG ACGGAGATTT CACAGAAGTG ACCATACAGG AGTACGACCA AGGGAAAGAT 
ACTTACTATG ACATCTACGA TGATAAGCTT CTCACTGACA CTTCAGACGG AATTGGTGGG 
CAGCCAGCTG AACAAGATCA ATTTGGCATT TCAGGTGGGG CCAGAGGTGA GAAAGGACAG 
AAGGGAGAGC CAGCCATCAT TGAACCCGGA ATGCTGATCG AAGGACCACC AGGCCCAGAA 
GGTCCAGCTG GTTATCCCGG TCCTCCTGGT ACCTCAGGTC CTCCTGGTCC AGCTGGAGAT 
CCTGGAGAGA GGGGCCCCCC TGGTCGTGCA GGTCTTCCTG GAGCTGATGG CTTGCCCGGA 
CCACCAGGAA CCATGCTGAT GTTGCCCTTC CGCTTCTCTG GTGGTGGAGA TGGTGGATCT 
AAAGGACCCC AAGTGTCTGC CCAGGAATCA CAAGCCCAAG CTATTCTCCA ACAAGCAAGA 
CTGGCGTTGA GAGGGCCATC TGGTCCGATG GGTCTTACTG GAAGACCAGG CCCATTGGGT 
TCTCCAGGTC CTTCAGGATT AAAGGGAGAG TCAGGAGATT TGGGACCTCA GGGGCCACGT 
GGGCCACAAG GTTCTCCCGG TCCATTAGGA AAACCAGGAC GAAGGGGACG AGCTGGTAGT 
GATGGTGCTC GGGGAATGCC TGGACAAACA GGACCAAAGG GTGACCGAGG CTTTGACGGG 
TTGGGCGGTT TGCCAGGAGA AAAGGGACAT CGTGGTGAGC CTGGTCCACA AGGTCCACCA 
GGGCCACATG GAGAAGACGG AGAAAGGGGT GATGATGGAG ACGTTGGACC TAGAGGGTTA 
CCAGGTGAAC CTGGACCCCG AGGGTTGCTT GGACCTAAAG GACCTCCTGG CCCACCGGGA 
ATCACCGGTG TTCCTGGAAT GGACGGTCAA GTTGGACCCA AAGGTAGTGT GGGACCTCAA 
GGTGAGCCAG GACCACCTGG GCAGCAAGGA AACCCAGGAG CACAGGGTCT TCCAGGTCCT 
CAGGGAGCTA TTGGACCACC AGGAGAGAAG GGACCACTGG GAAAATCAGG TCTTCCCGGA 
ATGCCCGGAG CTGATGGACC TCCCGGTCAC CCCGGGAAAG AAGGACCCTC AGGAGAGAAA 
GGAACCCTGG GTCCTGCAGG GCCACAGGGA CCAATTGGAT ACCCAGGACC ACGTGGTGTT 
AAGGGAGCTG ATGGAATTCG TGGTCTAAAG GGAACAAAAG GAGAAAAGGG TGAAGATGGA 
TTCCCTGGAT TTAAGGGTGA TATGGGCACC AAAGGAGACA GAGGTGAAAT CGGTCCTCCT 
GGTCCTAGAG GTGAAGATGG TCCTGAAGGT CCAAAGGGTC GAGGTGGTCC ATCTGGAGAA 
ATAGGTCCAC TTGGTCCTGG CGGTGAAAAG GGAAAGCTTG GTGTTCCTGG TCTACCAGGT 
TATCCAGGAA GACAAGGTCC AAAGGGTTCT ATCGGATTCC CAGGATTCGC AGGAGCAAAC 
GGAGAGAAAG GAACAAGGGG TACCCCTGGC AAACCGGGTC CAAGGGGGCA ACGAGGTCCA 
ACGGGTCCAC GTGGTGAAAG AGGTCCAAGG GGAGCTACAG GCAAGCCAGG CCCCAAGGGT 
AACTCCGGAG GGGATGGCCC AGCAGGTCCA CCTGGTGAAA GGGGACCACC TGGACCTCAA 
GGACCCACTG GATTTGCAGG ACCGAAGGGC CCCCCTGGTC CACCAGGGAA AGATGGACTT 
CCCGGCCACC CTGGACAGAG AGGAGAGACT GGATTCCAAG GAAAGACTGG CCCTCCTGGT 
CCCCCTGGCG TTGTCGGTCC TCAGGGTCCA ACTGGAGAGA CTGGCCCAAT GGGTGAAAGA 
GGACATCCTG GTCCTCCAGG TCCTCCTGGT GAGCAAGGTT TACCTGGTGT GGCTGGAAAA 
GAAGGATCCA AGGGAGACCC CGGTCCTGCT GGTTTGCCTG GAAAAGATGG ACCTCCAGGT 
CTAAGAGGTT TTACTGGAGA AAGAGGTCTC CCCGGTCCGA TTGGTGTTCC TGGACTGAAA 
GGCAATGAGG GTCCTCCTGG TCCACCTGGC CCAGCTGGTT CCCCCGGTGA AAGAGGAGCT 
GCGGGTCCAG CCGGACCTAT TGGACTGCCT GGAAGACCTG GCCCTCAGGG GCCACCAGGA 
CCTGCCGGCG AGAAGGGTAC ACCAGGTGAG AAAGGTCCCC AGGGACCTGC TGGACGTGAT 
GGTATCCAAG GCCCCGTTGG ACTTCCAGGC CCAGCTGGAC CTGTAGGGCC ACCTGGTGAA 
GATGGTGATA AGGGTGAAAT CGGAGAGCCT GGGCAAAAAG GAAACAGAGG AGATAAAGGA 
GAGCAGGGTC CCCCAGGTCC ACCTGGCCCA CAAGGTCCCA TTGGTCAGCC TGGCCCATCA 
GGATCTGATG GAGAGCCAGG CCCCAGGGGT CAACAAGGTC TTTTTGGACA AAAAGGTGAT 
GAAGGACCTA GAGGTTTTGC TGGACCACCA GGACCTGTTG GACTGCAGGG TTTACCTGGA 
CCACCTGGTG AGAAGGGAGA AACCGGAGAC GTTGGTCAGA TGGGTCCTCC TGGTCCCCCT 
GGTCCTAGAG GTCCTTCTGG CTCCCCTGGA GCTGATGGCC CACAAGGTCC TCCTGGAGGA 
ATTGGCAACC CTGGTCCTGT AGGAGAGAAG GGTGACCCTG GAGAGTCTGG GGAACCTGGT 
CTTCCTGGTG AACTTGGACC ACCGGGCCCT AAAGGTGAAA GAGGTGAAAA GGGAGAAGCG 
GGTCTTCAAG GAACTTCTGG CCCTCCAGGT CCTAAAGGTC CTCCAGGTGA TGATGGTCCA 
AAAGGTAGCC CTGGCCCAGT TGGATTTCCT GGTGATCCTG GTCCTCCTGG AGAATCTGGT 
CCTGCAGGTC AAGATGGTCC ACCTGGTGAT AAGGGAGAGG ACGGTGAATC TGGACAGCCA 
GGGTCACCAG GTCCTACTGG AGAGCCTGGG CCTTCAGGAC CACCTGGAAA GAGGGGTCCT 
CCTGGTCCAA CAGGTCCTGA AGGACGTCAA GGAGAAAAAG GAGCAAAGGG TGAATCAGGT 
TTGGAAGGAC CTCCTGGTAA GACTGGTCCT ATTGGTCCCC AAGGACCTCC CGGAAAACCT 
GGACCTGAAG GCCTTCGAGG AATTCCAGGC CCTGTTGGTG AACAAGGTCT CCCTGGTTCA 
CCTGGACCTG ATGGCCCTCC CGGACCTATG GGTCCACCAG GTATACCAGG TCTGAAAGGA 
GACTCAGGTC CAAAAGGAGA AAAGGGTCAT CCAGGCTTGA TAGGTCTCAT TGGACCTCCT 
GGTGAAACTA AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col5a1 ( XM_004916645.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916702.1 Xenopus tropicalis collagen, type V, alpha 1 (col5a1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa14033
Clone ID Related Accession (Same CDS sequence)	XM_004916645.3 , XM_004916645.4
Accession Version	XM_004916645.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-1(V) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683374.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004916645.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTATGG ATTCCCACAC ACGATGGAAA AGACGGAATT GGACGACAGA GTGGCCCCTC 
CTTGTAGCTT TAGCCTTGTC TATATGTTCC CGGTTCAGCG CAGCTGAACC GGCGGACCTG 
TTGAAGGCCT TAGAATTTCA CAATTTACCA GACGGTATCA AAAAAACGAC GGGCTTCTGC 
GCCTACCGCA AATCTTCAGA TGGACCAGAC GTGGCATTTA GAGTGTCCAA AGAGGCCCAG 
CTTAGCGCTC CGACCAATCA ATTGTTCTCC GATGTCGCAT TCCCAGAAGA TTTCTCCTTG 
TTGGCAACTG TGAAACCCAA AAAAGGAAGC CATTCCTACC TGCTCTCCAT CTACAATGAG 
CAAGGAACCC TGCAGATCGG CCTGACGCTG GGCTGGGAAC CGATGTTCGT CTATGAAGAC 
CACCAGGGAC ACCCACCCCC CGAGGATTAC CCCATCTTTA GAAACATTAA TCTGTCAGAT 
GGGAAGTGGC ACCGGGTAGC TTTCAGCGTG CACAAGAAGA CGATCACCCT GTTCCTGGAC 
TGCGTCAAGA AGGCCACCCT GCCCTTGAAA AGAAGCGACG GCCCCATTAT AGACACGAAC 
GGCATCGTCG TGTTCGGCAC CAGGATCCTG GACGAGCAAG TCTTTGAGGG AGACATTCAA 
CAGCTGATGA TCGTGCCCGA CGCCCGTGCT GCACATGATT ATTGTGAACA CTACACCCCC 
GACTGTGACA CCGCCATTCC AGATGCACCG CAGTCTCAGG ATCCCAACCA AGATGAATAT 
TACCCAGAAG CTGCTGAAGC CGAAGGCGAG GGTTATTACT ATGAATATCC TTACTACGAA 
GATGAGAAGA CAGAAACTGC AACTACCAAG CCCACGCCTG AGAAAGCCGA GGTACTAAGG 
CCCGAGACAG AAATCGAAGA GGAGGAAACA CCCCCACCAA CTGAAGCCCC TGCTATTCCC 
GTCACTGATG CTCCACGATT TGAGACCAGG GAAGAGAGCA GGGTAGAGAG CAGGGTAGAG 
AGCAGAGTGG ATAACCCCAC CACCGATGAC TACAACTATG AAACCGTACA GGAAGAATAT 
TACACCGCCT CTCCTTATGA AGATGTCAAC TACTCTGAAG GAGAGGAAAC AGGAATTGAG 
ACTGGGACTA CTGGTGAAGG CCTCATCGAG GGAGAAGGAG AAATTTTGAC CAAAACTGTC 
ATCACAAGCA GCTCATCCAA TGGCTCATCG GTCATCAGCA GTGGAAGTTC CAAAAAAGAA 
GGCAGTACCG ACGGAGATTT CACAGAAGTG ACCATACAGG AGTACGACCA AGGGAAAGAT 
ACTTACTATG ACATCTACGA TGATAAGCTT CTCACTGACA CTTCAGACGG AATTGGTGGG 
CAGCCAGCTG AACAAGATCA ATTTGGCATT TCAGGTGGGG CCAGAGGTGA GAAAGGACAG 
AAGGGAGAGC CAGCCATCAT TGAACCCGGA ATGCTGATCG AAGGACCACC AGGCCCAGAA 
GGTCCAGCTG GTTATCCCGG TCCTCCTGGT ACCTCAGGTC CTCCTGGTCC AGCTGGAGAT 
CCTGGAGAGA GGGGCCCCCC TGGTCGTGCA GGTCTTCCTG GAGCTGATGG CTTGCCCGGA 
CCACCAGGAA CCATGCTGAT GTTGCCCTTC CGCTTCTCTG GTGGTGGAGA TGGTGGATCT 
AAAGGACCCC AAGTGTCTGC CCAGGAATCA CAAGCCCAAG CTATTCTCCA ACAAGCAAGA 
CTGGCGTTGA GAGGGCCATC TGGTCCGATG GGTCTTACTG GAAGACCAGG CCCATTGGGT 
TCTCCAGGTC CTTCAGGATT AAAGGGAGAG TCAGGAGATT TGGGACCTCA GGGGCCACGT 
GGGCCACAAG GTTCTCCCGG TCCATTAGGA AAACCAGGAC GAAGGGGACG AGCTGGTAGT 
GATGGTGCTC GGGGAATGCC TGGACAAACA GGACCAAAGG GTGACCGAGG CTTTGACGGG 
TTGGGCGGTT TGCCAGGAGA AAAGGGACAT CGTGGTGAGC CTGGTCCACA AGGTCCACCA 
GGGCCACATG GAGAAGACGG AGAAAGGGGT GATGATGGAG ACGTTGGACC TAGAGGGTTA 
CCAGGTGAAC CTGGACCCCG AGGGTTGCTT GGACCTAAAG GACCTCCTGG CCCACCGGGA 
ATCACCGGTG TTCCTGGAAT GGACGGTCAA GTTGGACCCA AAGGTAGTGT GGGACCTCAA 
GGTGAGCCAG GACCACCTGG GCAGCAAGGA AACCCAGGAG CACAGGGTCT TCCAGGTCCT 
CAGGGAGCTA TTGGACCACC AGGAGAGAAG GGACCACTGG GAAAATCAGG TCTTCCCGGA 
ATGCCCGGAG CTGATGGACC TCCCGGTCAC CCCGGGAAAG AAGGACCCTC AGGAGAGAAA 
GGAACCCTGG GTCCTGCAGG GCCACAGGGA CCAATTGGAT ACCCAGGACC ACGTGGTGTT 
AAGGGAGCTG ATGGAATTCG TGGTCTAAAG GGAACAAAAG GAGAAAAGGG TGAAGATGGA 
TTCCCTGGAT TTAAGGGTGA TATGGGCACC AAAGGAGACA GAGGTGAAAT CGGTCCTCCT 
GGTCCTAGAG GTGAAGATGG TCCTGAAGGT CCAAAGGGTC GAGGTGGTCC ATCTGGAGAA 
ATAGGTCCAC TTGGTCCTGG CGGTGAAAAG GGAAAGCTTG GTGTTCCTGG TCTACCAGGT 
TATCCAGGAA GACAAGGTCC AAAGGGTTCT ATCGGATTCC CAGGATTCGC AGGAGCAAAC 
GGAGAGAAAG GAACAAGGGG TACCCCTGGC AAACCGGGTC CAAGGGGGCA ACGAGGTCCA 
ACGGGTCCAC GTGGTGAAAG AGGTCCAAGG GGAGCTACAG GCAAGCCAGG CCCCAAGGGT 
AACTCCGGAG GGGATGGCCC AGCAGGTCCA CCTGGTGAAA GGGGACCACC TGGACCTCAA 
GGACCCACTG GATTTGCAGG ACCGAAGGGC CCCCCTGGTC CACCAGGGAA AGATGGACTT 
CCCGGCCACC CTGGACAGAG AGGAGAGACT GGATTCCAAG GAAAGACTGG CCCTCCTGGT 
CCCCCTGGCG TTGTCGGTCC TCAGGGTCCA ACTGGAGAGA CTGGCCCAAT GGGTGAAAGA 
GGACATCCTG GTCCTCCAGG TCCTCCTGGT GAGCAAGGTT TACCTGGTGT GGCTGGAAAA 
GAAGGATCCA AGGGAGACCC CGGTCCTGCT GGTTTGCCTG GAAAAGATGG ACCTCCAGGT 
CTAAGAGGTT TTACTGGAGA AAGAGGTCTC CCCGGTCCGA TTGGTGTTCC TGGACTGAAA 
GGCAATGAGG GTCCTCCTGG TCCACCTGGC CCAGCTGGTT CCCCCGGTGA AAGAGGAGCT 
GCGGGTCCAG CCGGACCTAT TGGACTGCCT GGAAGACCTG GCCCTCAGGG GCCACCAGGA 
CCTGCCGGCG AGAAGGGTAC ACCAGGTGAG AAAGGTCCCC AGGGACCTGC TGGACGTGAT 
GGTATCCAAG GCCCCGTTGG ACTTCCAGGC CCAGCTGGAC CTGTAGGGCC ACCTGGTGAA 
GATGGTGATA AGGGTGAAAT CGGAGAGCCT GGGCAAAAAG GAAACAGAGG AGATAAAGGA 
GAGCAGGGTC CCCCAGGTCC ACCTGGCCCA CAAGGTCCCA TTGGTCAGCC TGGCCCATCA 
GGATCTGATG GAGAGCCAGG CCCCAGGGGT CAACAAGGTC TTTTTGGACA AAAAGGTGAT 
GAAGGACCTA GAGGTTTTGC TGGACCACCA GGACCTGTTG GACTGCAGGG TTTACCTGGA 
CCACCTGGTG AGAAGGGAGA AACCGGAGAC GTTGGTCAGA TGGGTCCTCC TGGTCCCCCT 
GGTCCTAGAG GTCCTTCTGG CTCCCCTGGA GCTGATGGCC CACAAGGTCC TCCTGGAGGA 
ATTGGCAACC CTGGTCCTGT AGGAGAGAAG GGTGACCCTG GAGAGTCTGG GGAACCTGGT 
CTTCCTGGTG AACTTGGACC ACCGGGCCCT AAAGGTGAAA GAGGTGAAAA GGGAGAAGCG 
GGTCTTCAAG GAACTTCTGG CCCTCCAGGT CCTAAAGGTC CTCCAGGTGA TGATGGTCCA 
AAAGGTAGCC CTGGCCCAGT TGGATTTCCT GGTGATCCTG GTCCTCCTGG AGAATCTGGT 
CCTGCAGGTC AAGATGGTCC ACCTGGTGAT AAGGGAGAGG ACGGTGAATC TGGACAGCCA 
GGGTCACCAG GTCCTACTGG AGAGCCTGGG CCTTCAGGAC CACCTGGAAA GAGGGGTCCT 
CCTGGTCCAA CAGGTCCTGA AGGACGTCAA GGAGAAAAAG GAGCAAAGGG TGAATCAGGT 
TTGGAAGGAC CTCCTGGTAA GACTGGTCCT ATTGGTCCCC AAGGACCTCC CGGAAAACCT 
GGACCTGAAG GCCTTCGAGG AATTCCAGGC CCTGTTGGTG AACAAGGTCT CCCTGGTTCA 
CCTGGACCTG ATGGCCCTCC CGGACCTATG GGTCCACCAG GTATACCAGG TCTGAAAGGA 
GACTCAGGTC CAAAAGGAGA AAAGGGTCAT CCAGGCTTGA TAGGTCTCAT TGGACCTCCT 
GGTGAACAGG GAGAAAAGGG AGACCGTGGT TTGCCAGGCC CCCAGGGACC ACATGGTGCT 
AAAGGAGAAC AGGGTATTCT TGGTCCATCT GGTCCTGTTG GTCCACCTGG TCCTCCAGGC 
TTACCGGGTG GTCCTGGTCC AAAAGGTGCT AAAGGTTCAT CGGGTCCAAC TGGTCCAAAG 
GGTGAATCTG GAATCCCTGG TCCACCAGGT CCTCCAGGAC CTCCAGGCGA GGTGATCCAG 
CCTCTGCCTA TCCATTCCTC AAAGAGGACA AGACGTAGCA TTGATGCAAG TCAAGTAGTG 
GACGATGGAA ATGCAGACAA CTACATGGAC TATGCCGATG GAATGGAGGA AATATTTGGT 
TCTCTCAACT CCCTAAAACA GGAAATTGAA CAAATGAAAC ACCCACTGGG CAATCAGCAG 
AACCCAGCAA GGACTTGCAA GGACCTGCTT CTGTGCCATC CTGAACTCCC AGATGGGGAA 
TATTGGATTG ACCCAAATCA AGGATGCTCT AGAGACTCGT TCAAGGTTTT CTGTAATTTC 
ACAGCCGGTG GAGAATCTTG TATCTTCCCC GACAAGAAGT CTGAAGGAGC TAGAATTACA 
TCTTGGCCCA AGGAAAATCC AGGCTCCTGG TTCAGTGAAT TCAAGCGTGG TAAACTGTTG 
TCCTACGTTG ATTCAAATGG CAACCCCATT GGAGTGGTGC AGATGACCTT CATGAAGCTT 
CTGAGTGCTT CGGTGAAACA GAATATCACC TACAACTGCT ACCAGTCCGT GGCATGGCAC 
GACGCCACCA CCAACAGCTA CGACAAGGCC ATTCGCTTCC TGGGGTCCAA CGACGAAGAA 
ATGTCCTACG ACAACAATCC TTCCATTCGA GCCATTATGG ATGGATGTGC GTCAAAGAAA 
GGTTATCAGA AGACAGTTCT GGAACTCGAC ACGTCCAAAG TGGAACAGGC CCCCATTGTG 
GATATTATGT TCAACGACTT CGGAGATCCA GTACAAAAAT TCGGCTTTGA GGTGGGACCA 
GTGTGTTTCA TGGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916702.1
CDS	342..5939
Translation	MSMDSHTRWKRRNWTTEWPLLVALALSICSRFSAAEPADLLKALEFHNLPDGIKKTTGFCAYRKSSDGPDVAFRVSKEAQLSAPTNQLFSDVAFPEDFSLLATVKPKKGSHSYLLSIYNEQGTLQIGLTLGWEPMFVYEDHQGHPPPEDYPIFRNINLSDGKWHRVAFSVHKKTITLFLDCVKKATLPLKRSDGPIIDTNGIVVFGTRILDEQVFEGDIQQLMIVPDARAAHDYCEHYTPDCDTAIPDAPQSQDPNQDEYYPEAAEAEGEGYYYEYPYYEDEKTETATTKPTPEKAEVLRPETEIEEEETPPPTEAPAIPVTDAPRFETREESRVESRVESRVDNPTTDDYNYETVQEEYYTASPYEDVNYSEGEETGIETGTTGEGLIEGEGEILTKTVITSSSSNGSSVISSGSSKKEGSTDGDFTEVTIQEYDQGKDTYYDIYDDKLLTDTSDGIGGQPAEQDQFGISGGARGEKGQKGEPAIIEPGMLIEGPPGPEGPAGYPGPPGTSGPPGPAGDPGERGPPGRAGLPGADGLPGPPGTMLMLPFRFSGGGDGGSKGPQVSAQESQAQAILQQARLALRGPSGPMGLTGRPGPLGSPGPSGLKGESGDLGPQGPRGPQGSPGPLGKPGRRGRAGSDGARGMPGQTGPKGDRGFDGLGGLPGEKGHRGEPGPQGPPGPHGEDGERGDDGDVGPRGLPGEPGPRGLLGPKGPPGPPGITGVPGMDGQVGPKGSVGPQGEPGPPGQQGNPGAQGLPGPQGAIGPPGEKGPLGKSGLPGMPGADGPPGHPGKEGPSGEKGTLGPAGPQGPIGYPGPRGVKGADGIRGLKGTKGEKGEDGFPGFKGDMGTKGDRGEIGPPGPRGEDGPEGPKGRGGPSGEIGPLGPGGEKGKLGVPGLPGYPGRQGPKGSIGFPGFAGANGEKGTRGTPGKPGPRGQRGPTGPRGERGPRGATGKPGPKGNSGGDGPAGPPGERGPPGPQGPTGFAGPKGPPGPPGKDGLPGHPGQRGETGFQGKTGPPGPPGVVGPQGPTGETGPMGERGHPGPPGPPGEQGLPGVAGKEGSKGDPGPAGLPGKDGPPGLRGFTGERGLPGPIGVPGLKGNEGPPGPPGPAGSPGERGAAGPAGPIGLPGRPGPQGPPGPAGEKGTPGEKGPQGPAGRDGIQGPVGLPGPAGPVGPPGEDGDKGEIGEPGQKGNRGDKGEQGPPGPPGPQGPIGQPGPSGSDGEPGPRGQQGLFGQKGDEGPRGFAGPPGPVGLQGLPGPPGEKGETGDVGQMGPPGPPGPRGPSGSPGADGPQGPPGGIGNPGPVGEKGDPGESGEPGLPGELGPPGPKGERGEKGEAGLQGTSGPPGPKGPPGDDGPKGSPGPVGFPGDPGPPGESGPAGQDGPPGDKGEDGESGQPGSPGPTGEPGPSGPPGKRGPPGPTGPEGRQGEKGAKGESGLEGPPGKTGPIGPQGPPGKPGPEGLRGIPGPVGEQGLPGSPGPDGPPGPMGPPGIPGLKGDSGPKGEKGHPGLIGLIGPPGEQGEKGDRGLPGPQGPHGAKGEQGILGPSGPVGPPGPPGLPGGPGPKGAKGSSGPTGPKGESGIPGPPGPPGPPGEVIQPLPIHSSKRTRRSIDASQVVDDGNADNYMDYADGMEEIFGSLNSLKQEIEQMKHPLGNQQNPARTCKDLLLCHPELPDGEYWIDPNQGCSRDSFKVFCNFTAGGESCIFPDKKSEGARITSWPKENPGSWFSEFKRGKLLSYVDSNGNPIGVVQMTFMKLLSASVKQNITYNCYQSVAWHDATTNSYDKAIRFLGSNDEEMSYDNNPSIRAIMDGCASKKGYQKTVLELDTSKVEQAPIVDIMFNDFGDPVQKFGFEVGPVCFMG

Target ORF information:

RefSeq Version	XM_004916645.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen, type V, alpha 1 (col5a1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916645.3

ORF Insert Sequence:

ATGAGTATGG ATTCCCACAC ACGATGGAAA AGACGGAATT GGACGACAGA GTGGCCCCTC 
CTTGTAGCTT TAGCCTTGTC TATATGTTCC CGGTTCAGCG CAGCTGAACC GGCGGACCTG 
TTGAAGGCCT TAGAATTTCA CAATTTACCA GACGGTATCA AAAAAACGAC GGGCTTCTGC 
GCCTACCGCA AATCTTCAGA TGGACCAGAC GTGGCATTTA GAGTGTCCAA AGAGGCCCAG 
CTTAGCGCTC CGACCAATCA ATTGTTCTCC GATGTCGCAT TCCCAGAAGA TTTCTCCTTG 
TTGGCAACTG TGAAACCCAA AAAAGGAAGC CATTCCTACC TGCTCTCCAT CTACAATGAG 
CAAGGAACCC TGCAGATCGG CCTGACGCTG GGCTGGGAAC CGATGTTCGT CTATGAAGAC 
CACCAGGGAC ACCCACCCCC CGAGGATTAC CCCATCTTTA GAAACATTAA TCTGTCAGAT 
GGGAAGTGGC ACCGGGTAGC TTTCAGCGTG CACAAGAAGA CGATCACCCT GTTCCTGGAC 
TGCGTCAAGA AGGCCACCCT GCCCTTGAAA AGAAGCGACG GCCCCATTAT AGACACGAAC 
GGCATCGTCG TGTTCGGCAC CAGGATCCTG GACGAGCAAG TCTTTGAGGG AGACATTCAA 
CAGCTGATGA TCGTGCCCGA CGCCCGTGCT GCACATGATT ATTGTGAACA CTACACCCCC 
GACTGTGACA CCGCCATTCC AGATGCACCG CAGTCTCAGG ATCCCAACCA AGATGAATAT 
TACCCAGAAG CTGCTGAAGC CGAAGGCGAG GGTTATTACT ATGAATATCC TTACTACGAA 
GATGAGAAGA CAGAAACTGC AACTACCAAG CCCACGCCTG AGAAAGCCGA GGTACTAAGG 
CCCGAGACAG AAATCGAAGA GGAGGAAACA CCCCCACCAA CTGAAGCCCC TGCTATTCCC 
GTCACTGATG CTCCACGATT TGAGACCAGG GAAGAGAGCA GGGTAGAGAG CAGGGTAGAG 
AGCAGAGTGG ATAACCCCAC CACCGATGAC TACAACTATG AAACCGTACA GGAAGAATAT 
TACACCGCCT CTCCTTATGA AGATGTCAAC TACTCTGAAG GAGAGGAAAC AGGAATTGAG 
ACTGGGACTA CTGGTGAAGG CCTCATCGAG GGAGAAGGAG AAATTTTGAC CAAAACTGTC 
ATCACAAGCA GCTCATCCAA TGGCTCATCG GTCATCAGCA GTGGAAGTTC CAAAAAAGAA 
GGCAGTACCG ACGGAGATTT CACAGAAGTG ACCATACAGG AGTACGACCA AGGGAAAGAT 
ACTTACTATG ACATCTACGA TGATAAGCTT CTCACTGACA CTTCAGACGG AATTGGTGGG 
CAGCCAGCTG AACAAGATCA ATTTGGCATT TCAGGTGGGG CCAGAGGTGA GAAAGGACAG 
AAGGGAGAGC CAGCCATCAT TGAACCCGGA ATGCTGATCG AAGGACCACC AGGCCCAGAA 
GGTCCAGCTG GTTATCCCGG TCCTCCTGGT ACCTCAGGTC CTCCTGGTCC AGCTGGAGAT 
CCTGGAGAGA GGGGCCCCCC TGGTCGTGCA GGTCTTCCTG GAGCTGATGG CTTGCCCGGA 
CCACCAGGAA CCATGCTGAT GTTGCCCTTC CGCTTCTCTG GTGGTGGAGA TGGTGGATCT 
AAAGGACCCC AAGTGTCTGC CCAGGAATCA CAAGCCCAAG CTATTCTCCA ACAAGCAAGA 
CTGGCGTTGA GAGGGCCATC TGGTCCGATG GGTCTTACTG GAAGACCAGG CCCATTGGGT 
TCTCCAGGTC CTTCAGGATT AAAGGGAGAG TCAGGAGATT TGGGACCTCA GGGGCCACGT 
GGGCCACAAG GTTCTCCCGG TCCATTAGGA AAACCAGGAC GAAGGGGACG AGCTGGTAGT 
GATGGTGCTC GGGGAATGCC TGGACAAACA GGACCAAAGG GTGACCGAGG CTTTGACGGG 
TTGGGCGGTT TGCCAGGAGA AAAGGGACAT CGTGGTGAGC CTGGTCCACA AGGTCCACCA 
GGGCCACATG GAGAAGACGG AGAAAGGGGT GATGATGGAG ACGTTGGACC TAGAGGGTTA 
CCAGGTGAAC CTGGACCCCG AGGGTTGCTT GGACCTAAAG GACCTCCTGG CCCACCGGGA 
ATCACCGGTG TTCCTGGAAT GGACGGTCAA GTTGGACCCA AAGGTAGTGT GGGACCTCAA 
GGTGAGCCAG GACCACCTGG GCAGCAAGGA AACCCAGGAG CACAGGGTCT TCCAGGTCCT 
CAGGGAGCTA TTGGACCACC AGGAGAGAAG GGACCACTGG GAAAATCAGG TCTTCCCGGA 
ATGCCCGGAG CTGATGGACC TCCCGGTCAC CCCGGGAAAG AAGGACCCTC AGGAGAGAAA 
GGAACCCTGG GTCCTGCAGG GCCACAGGGA CCAATTGGAT ACCCAGGACC ACGTGGTGTT 
AAGGGAGCTG ATGGAATTCG TGGTCTAAAG GGAACAAAAG GAGAAAAGGG TGAAGATGGA 
TTCCCTGGAT TTAAGGGTGA TATGGGCACC AAAGGAGACA GAGGTGAAAT CGGTCCTCCT 
GGTCCTAGAG GTGAAGATGG TCCTGAAGGT CCAAAGGGTC GAGGTGGTCC ATCTGGAGAA 
ATAGGTCCAC TTGGTCCTGG CGGTGAAAAG GGAAAGCTTG GTGTTCCTGG TCTACCAGGT 
TATCCAGGAA GACAAGGTCC AAAGGGTTCT ATCGGATTCC CAGGATTCGC AGGAGCAAAC 
GGAGAGAAAG GAACAAGGGG TACCCCTGGC AAACCGGGTC CAAGGGGGCA ACGAGGTCCA 
ACGGGTCCAC GTGGTGAAAG AGGTCCAAGG GGAGCTACAG GCAAGCCAGG CCCCAAGGGT 
AACTCCGGAG GGGATGGCCC AGCAGGTCCA CCTGGTGAAA GGGGACCACC TGGACCTCAA 
GGACCCACTG GATTTGCAGG ACCGAAGGGC CCCCCTGGTC CACCAGGGAA AGATGGACTT 
CCCGGCCACC CTGGACAGAG AGGAGAGACT GGATTCCAAG GAAAGACTGG CCCTCCTGGT 
CCCCCTGGCG TTGTCGGTCC TCAGGGTCCA ACTGGAGAGA CTGGCCCAAT GGGTGAAAGA 
GGACATCCTG GTCCTCCAGG TCCTCCTGGT GAGCAAGGTT TACCTGGTGT GGCTGGAAAA 
GAAGGATCCA AGGGAGACCC CGGTCCTGCT GGTTTGCCTG GAAAAGATGG ACCTCCAGGT 
CTAAGAGGTT TTACTGGAGA AAGAGGTCTC CCCGGTCCGA TTGGTGTTCC TGGACTGAAA 
GGCAATGAGG GTCCTCCTGG TCCACCTGGC CCAGCTGGTT CCCCCGGTGA AAGAGGAGCT 
GCGGGTCCAG CCGGACCTAT TGGACTGCCT GGAAGACCTG GCCCTCAGGG GCCACCAGGA 
CCTGCCGGCG AGAAGGGTAC ACCAGGTGAG AAAGGTCCCC AGGGACCTGC TGGACGTGAT 
GGTATCCAAG GCCCCGTTGG ACTTCCAGGC CCAGCTGGAC CTGTAGGGCC ACCTGGTGAA 
GATGGTGATA AGGGTGAAAT CGGAGAGCCT GGGCAAAAAG GAAACAGAGG AGATAAAGGA 
GAGCAGGGTC CCCCAGGTCC ACCTGGCCCA CAAGGTCCCA TTGGTCAGCC TGGCCCATCA 
GGATCTGATG GAGAGCCAGG CCCCAGGGGT CAACAAGGTC TTTTTGGACA AAAAGGTGAT 
GAAGGACCTA GAGGTTTTGC TGGACCACCA GGACCTGTTG GACTGCAGGG TTTACCTGGA 
CCACCTGGTG AGAAGGGAGA AACCGGAGAC GTTGGTCAGA TGGGTCCTCC TGGTCCCCCT 
GGTCCTAGAG GTCCTTCTGG CTCCCCTGGA GCTGATGGCC CACAAGGTCC TCCTGGAGGA 
ATTGGCAACC CTGGTCCTGT AGGAGAGAAG GGTGACCCTG GAGAGTCTGG GGAACCTGGT 
CTTCCTGGTG AACTTGGACC ACCGGGCCCT AAAGGTGAAA GAGGTGAAAA GGGAGAAGCG 
GGTCTTCAAG GAACTTCTGG CCCTCCAGGT CCTAAAGGTC CTCCAGGTGA TGATGGTCCA 
AAAGGTAGCC CTGGCCCAGT TGGATTTCCT GGTGATCCTG GTCCTCCTGG AGAATCTGGT 
CCTGCAGGTC AAGATGGTCC ACCTGGTGAT AAGGGAGAGG ACGGTGAATC TGGACAGCCA 
GGGTCACCAG GTCCTACTGG AGAGCCTGGG CCTTCAGGAC CACCTGGAAA GAGGGGTCCT 
CCTGGTCCAA CAGGTCCTGA AGGACGTCAA GGAGAAAAAG GAGCAAAGGG TGAATCAGGT 
TTGGAAGGAC CTCCTGGTAA GACTGGTCCT ATTGGTCCCC AAGGACCTCC CGGAAAACCT 
GGACCTGAAG GCCTTCGAGG AATTCCAGGC CCTGTTGGTG AACAAGGTCT CCCTGGTTCA 
CCTGGACCTG ATGGCCCTCC CGGACCTATG GGTCCACCAG GTATACCAGG TCTGAAAGGA 
GACTCAGGTC CAAAAGGAGA AAAGGGTCAT CCAGGCTTGA TAGGTCTCAT TGGACCTCCT 
GGTGAACAGG GAGAAAAGGG AGACCGTGGT TTGCCAGGCC CCCAGGGACC ACATGGTGCT 
AAAGGAGAAC AGGGTATTCT TGGTCCATCT GGTCCTGTTG GTCCACCTGG TCCTCCAGGC 
TTACCGGGTG GTCCTGGTCC AAAAGGTGCT AAAGGTTCAT CGGGTCCAAC TGGTCCAAAG 
GGTGAATCTG GAATCCCTGG TCCACCAGGT CCTCCAGGAC CTCCAGGCGA GGTGATCCAG 
CCTCTGCCTA TCCATTCCTC AAAGAGGACA AGACGTAGCA TTGATGCAAG TCAAGTAGTG 
GACGATGGAA ATGCAGACAA CTACATGGAC TATGCCGATG GAATGGAGGA AATATTTGGT 
TCTCTCAACT CCCTAAAACA GGAAATTGAA CAAATGAAAC ACCCACTGGG CAATCAGCAG 
AACCCAGCAA GGACTTGCAA GGACCTGCTT CTGTGCCATC CTGAACTCCC AGATGGGGAA 
TATTGGATTG ACCCAAATCA AGGATGCTCT AGAGACTCGT TCAAGGTTTT CTGTAATTTC 
ACAGCCGGTG GAGAATCTTG TATCTTCCCC GACAAGAAGT CTGAAGGAGC TAGAATTACA 
TCTTGGCCCA AGGAAAATCC AGGCTCCTGG TTCAGTGAAT TCAAGCGTGG TAAACTGTTG 
TCCTACGTTG ATTCAAATGG CAACCCCATT GGAGTGGTGC AGATGACCTT CATGAAGCTT 
CTGAGTGCTT CGGTGAAACA GAATATCACC TACAACTGCT ACCAGTCCGT GGCATGGCAC 
GACGCCACCA CCAACAGCTA CGACAAGGCC ATTCGCTTCC TGGGGTCCAA CGACGAAGAA 
ATGTCCTACG ACAACAATCC TTCCATTCGA GCCATTATGG ATGGATGTGC GTCAAAGAAA 
GGTTATCAGA AGACAGTTCT GGAACTCGAC ACGTCCAAAG TGGAACAGGC CCCCATTGTG 
GATATTATGT TCAACGACTT CGGAGATCCA GTACAAAAAT TCGGCTTTGA GGTGGGACCA 
GTGTGTTTCA TGGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col5a1 ( XM_012969175.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824629.1 Xenopus tropicalis collagen, type V, alpha 1 (col5a1), transcript variant X2, mRNA.

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CloneID	OXa30187
Clone ID Related Accession (Same CDS sequence)	XM_012969175.2 , XM_012969175.3
Accession Version	XM_012969175.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-1(V) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683374.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012969175.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTATGG ATTCCCACAC ACGATGGAAA AGACGGAATT GGACGACAGA GTGGCCCCTC 
CTTGTAGCTT TAGCCTTGTC TATATGTTCC CGGTTCAGCG CAGCTGAACC GGCGGACCTG 
TTGAAGGCCT TAGAATTTCA CAATTTACCA GACGGTATCA AAAAAACGAC GGGCTTCTGC 
GCCTACCGCA AATCTTCAGA TGGACCAGAC GTGGCATTTA GAGTGTCCAA AGAGGCCCAG 
CTTAGCGCTC CGACCAATCA ATTGTTCTCC GATGTCGCAT TCCCAGAAGA TTTCTCCTTG 
TTGGCAACTG TGAAACCCAA AAAAGGAAGC CATTCCTACC TGCTCTCCAT CTACAATGAG 
CAAGGAACCC TGCAGATCGG CCTGACGCTG GGCTGGGAAC CGATGTTCGT CTATGAAGAC 
CACCAGGGAC ACCCACCCCC CGAGGATTAC CCCATCTTTA GAAACATTAA TCTGTCAGAT 
GGGAAGTGGC ACCGGGTAGC TTTCAGCGTG CACAAGAAGA CGATCACCCT GTTCCTGGAC 
TGCGTCAAGA AGGCCACCCT GCCCTTGAAA AGAAGCGACG GCCCCATTAT AGACACGAAC 
GGCATCGTCG TGTTCGGCAC CAGGATCCTG GACGAGCAAG TCTTTGAGGG AGACATTCAA 
CAGCTGATGA TCGTGCCCGA CGCCCGTGCT GCACATGATT ATTGTGAACA CTACACCCCC 
GACTGTGACA CCGCCATTCC AGATGCACCG CAGTCTCAGG ATCCCAACCA AGATGAATAT 
TACCCAGAAG CTGCTGAAGC CGAAGGCGAG GGTTATTACT ATGAATATCC TTACTACGAA 
GATGAGAAGA CAGAAACTGC AACTACCAAG CCCACGCCTG AGAAAGCCGA GGTACTAAGG 
CCCGAGACAG AAATCGAAGA GGAGGAAACA CCCCCACCAA CTGAAGCCCC TGCTATTCCC 
GTCACTGATG CTCCACGATT TGAGACCAGG GAAGAGAGCA GGGTAGAGAG CAGGGTAGAG 
AGCAGAGTGG ATAACCCCAC CACCGATGAC TACAACTATG AAACCGTACA GGAAGAATAT 
TACACCGCCT CTCCTTATGA AGATGTCAAC TACTCTGAAG GAGAGGAAAC AGGAATTGAG 
ACTGGGACTA CTGGTGAAGG CCTCATCGAG GGAGAAGGAG AAATTTTGAC CAAAACTGTC 
ATCACAAGCA GCTCATCCAA TGGCTCATCG GTCATCAGCA GTGGAAGTTC CAAAAAAGAA 
GGCAGTACCG ACGGAGATTT CACAGAAGTG ACCATACAGG AGTACGACCA AGGGAAAGAT 
ACTTACTATG ACATCTACGA TGATAAGCTT CTCACTGACA CTTCAGACGG AATTGGTGGG 
CAGCCAGCTG AACAAGATCA ATTTGGCATT TCAGGTGGGG CCAGAGGTGA GAAAGGACAG 
AAGGGAGAGC CAGCCATCAT TGAACCCGGA ATGCTGATCG AAGGACCACC AGGCCCAGAA 
GGTCCAGCTG GTTATCCCGG TCCTCCTGGT ACCTCAGGTC CTCCTGGTCC AGCTGGAGAT 
CCTGGAGAGA GGGGCCCCCC TGGTCGTGCA GGTCTTCCTG GAGCTGATGG CTTGCCCGGA 
CCACCAGGAA CCATGCTGAT GTTGCCCTTC CGCTTCTCTG GTGGTGGAGA TGGTGGATCT 
AAAGGACCCC AAGTGTCTGC CCAGGAATCA CAAGCCCAAG CTATTCTCCA ACAAGCAAGA 
CTGGCGTTGA GAGGGCCATC TGGTCCGATG GGTCTTACTG GAAGACCAGG CCCATTGGGT 
TCTCCAGGTC CTTCAGGATT AAAGGGAGAG TCAGGAGATT TGGGACCTCA GGGGCCACGT 
GGGCCACAAG GTTCTCCCGG TCCATTAGGA AAACCAGGAC GAAGGGGACG AGCTGGTAGT 
GATGGTGCTC GGGGAATGCC TGGACAAACA GGACCAAAGG GTGACCGAGG CTTTGACGGG 
TTGGGCGGTT TGCCAGGAGA AAAGGGACAT CGTGGTGAGC CTGGTCCACA AGGTCCACCA 
GGGCCACATG GAGAAGACGG AGAAAGGGGT GATGATGGAG ACGTTGGACC TAGAGGGTTA 
CCAGGTGAAC CTGGACCCCG AGGGTTGCTT GGACCTAAAG GACCTCCTGG CCCACCGGGA 
ATCACCGGTG TTCCTGGAAT GGACGGTCAA GTTGGACCCA AAGGTAGTGT GGGACCTCAA 
GGTGAGCCAG GACCACCTGG GCAGCAAGGA AACCCAGGAG CACAGGGTCT TCCAGGTCCT 
CAGGGAGCTA TTGGACCACC AGGAGAGAAG GGACCACTGG GAAAATCAGG TCTTCCCGGA 
ATGCCCGGAG CTGATGGACC TCCCGGTCAC CCCGGGAAAG AAGGACCCTC AGGAGAGAAA 
GGAACCCTGG GTCCTGCAGG GCCACAGGGA CCAATTGGAT ACCCAGGACC ACGTGGTGTT 
AAGGGAGCTG ATGGAATTCG TGGTCTAAAG GGAACAAAAG GAGAAAAGGG TGAAGATGGA 
TTCCCTGGAT TTAAGGGTGA TATGGGCACC AAAGGAGACA GAGGTGAAAT CGGTCCTCCT 
GGTCCTAGAG GTGAAGATGG TCCTGAAGGT CCAAAGGGTC GAGGTGGTCC ATCTGGAGAA 
ATAGGTCCAC TTGGTCCTGG CGGTGAAAAG GGAAAGCTTG GTGTTCCTGG TCTACCAGGT 
TATCCAGGAA GACAAGGTCC AAAGGGTTCT ATCGGATTCC CAGGATTCGC AGGAGCAAAC 
GGAGAGAAAG GAACAAGGGG TACCCCTGGC AAACCGGGTC CAAGGGGGCA ACGAGGTCCA 
ACGGGTCCAC GTGGTGAAAG AGGTCCAAGG GGAGCTACAG GCAAGCCAGG CCCCAAGGGT 
AACTCCGGAG GGGATGGCCC AGCAGGTCCA CCTGGTGAAA GGGGACCACC TGGACCTCAA 
GGACCCACTG GATTTGCAGG ACCGAAGGGC CCCCCTGGTC CACCAGGGAA AGATGGACTT 
CCCGGCCACC CTGGACAGAG AGGAGAGACT GGATTCCAAG GAAAGACTGG CCCTCCTGGT 
CCCCCTGGCG TTGTCGGTCC TCAGGGTCCA ACTGGAGAGA CTGGCCCAAT GGGTGAAAGA 
GGACATCCTG GTCCTCCAGG TCCTCCTGGT GAGCAAGGTT TACCTGGTGT GGCTGGAAAA 
GAAGGATCCA AGGGAGACCC CGGTCCTGCT GGTTTGCCTG GAAAAGATGG ACCTCCAGGT 
CTAAGAGGTT TTACTGGAGA AAGAGGTCTC CCCGGTCCGA TTGGTGTTCC TGGACTGAAA 
GGCAATGAGG GTCCTCCTGG TCCACCTGGC CCAGCTGGTT CCCCCGGTGA AAGAGGAGCT 
GCGGGTCCAG CCGGACCTAT TGGACTGCCT GGAAGACCTG GCCCTCAGGG GCCACCAGGA 
CCTGCCGGCG AGAAGGGTAC ACCAGGTGAG AAAGGTCCCC AGGGACCTGC TGGACGTGAT 
GGTATCCAAG GCCCCGTTGG ACTTCCAGGC CCAGCTGGAC CTGTAGGGCC ACCTGGTGAA 
GATGGTGATA AGGGTGAAAT CGGAGAGCCT GGGCAAAAAG GAAACAGAGG AGATAAAGGA 
GAGCAGGGTC CCCCAGGTCC ACCTGGCCCA CAAGGTCCCA TTGGTCAGCC TGGCCCATCA 
GGATCTGATG GAGAGCCAGG CCCCAGGGGT CAACAAGGTC TTTTTGGACA AAAAGGTGAT 
GAAGGACCTA GAGGTTTTGC TGGACCACCA GGACCTGTTG GACTGCAGGG TTTACCTGGA 
CCACCTGGTG AGAAGGGAGA AACCGGAGAC GTTGGTCAGA TGGGTCCTCC TGGTCCCCCT 
GGTCCTAGAG GTCCTTCTGG CTCCCCTGGA GCTGATGGCC CACAAGGTCC TCCTGGAGGA 
ATTGGCAACC CTGGTCCTGT AGGAGAGAAG GGTGACCCTG GAGAGTCTGG GGAACCTGGT 
CTTCCTGGTG AACTTGGACC ACCGGGCCCT AAAGGTGAAA GAGGTGAAAA GGGAGAAGCG 
GGTCTTCAAG GAACTTCTGG CCCTCCAGGT CCTAAAGGTC CTCCAGGTGA TGATGGTCCA 
AAAGGTAGCC CTGGCCCAGT TGGATTTCCT GGTGATCCTG GTCCTCCTGG AGAATCTGGT 
CCTGCAGGTC AAGATGGTCC ACCTGGTGAT AAGGGAGAGG ACGGTGAATC TGGACAGCCA 
GGGTCACCAG GTCCTACTGG AGAGCCTGGG CCTTCAGGAC CACCTGGAAA GAGGGGTCCT 
CCTGGTCCAA CAGGTCCTGA AGGACGTCAA GGAGAAAAAG GAGCAAAGGG TGAATCAGGT 
TTGGAAGGAC CTCCTGGTAA GACTGGTCCT ATTGGTCCCC AAGGACCTCC CGGAAAACCT 
GGACCTGAAG GCCTTCGAGG AATTCCAGGC CCTGTTGGTG AACAAGGTCT CCCTGGTTCA 
CCTGGACCTG ATGGCCCTCC CGGACCTATG GGTCCACCAG GTATACCAGG TCTGAAAGGA 
GACTCAGGTC CAAAAGGAGA AAAGGGTCAT CCAGGCTTGA TAGGTCTCAT TGGACCTCCT 
GGTGAACAGG GAGAAAAGGG AGACCGTGGT TTGCCAGGCC CCCAGGGACC ACATGGTGCT 
AAAGGAGAAC AGGGTATTCT TGGTCCATCT GGTCCTGTTG GTCCACCTGG TCCTCCAGGC 
TTACCGGGTG GTCCTGGTCC AAAAGGTGCT AAAGGTTCAT CGGGTCCAAC TGGTCCAAAG 
GGTGAATCTG GAATCCCTGG TCCACCAGGT CCTCCAGGAC CTCCAGGCGA GGTGATCCAG 
CCTCTGCCTA TCCATTCCTC AAAGAGGACA AGACGTAGCA TTGATGCAAG TCAAGTAGTG 
GACGATGGAA ATGCAGACAA CTACATGGAC TATGCCGATG GAATGGAGGA AATATTTGGT 
TCTCTCAACT CCCTAAAACA GGAAATTGAA CAAATGAAAC ACCCACTGGG CAATCAGCAG 
AACCCAGCAA GGACTTGCAA GGACCTGCTT CTGTGCCATC CTGAACTCCC AGATGGGGAA 
TATTGGATTG ACCCAAATCA AGGATGCTCT AGAGACTCGT TCAAGGTTTT CTGTAATTTC 
ACAGCCGGTG GAGAATCTTG TATCTTCCCC GACAAGAAGT CTGAAGGAAA TAAACTGGCT 
CGTTGGCCCA AAGAGCTGCC TGGTACATGG TATAGTCATT ACAAGCGGGG TTCCATGTTG 
TCCTACGTTG ATTCAAATGG CAACCCCATT GGAGTGGTGC AGATGACCTT CATGAAGCTT 
CTGAGTGCTT CGGTGAAACA GAATATCACC TACAACTGCT ACCAGTCCGT GGCATGGCAC 
GACGCCACCA CCAACAGCTA CGACAAGGCC ATTCGCTTCC TGGGGTCCAA CGACGAAGAA 
ATGTCCTACG ACAACAATCC TTCCATTCGA GCCATTATGG ATGGATGTGC GTCAAAGAAA 
GGTTATCAGA AGACAGTTCT GGAACTCGAC ACGTCCAAAG TGGAACAGGC CCCCATTGTG 
GATATTATGT TCAACGACTT CGGAGATCCA GTACAAAAAT TCGGCTTTGA GGTGGGACCA 
GTGTGTTTCA TGGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824629.1
CDS	342..5939
Translation	MSMDSHTRWKRRNWTTEWPLLVALALSICSRFSAAEPADLLKALEFHNLPDGIKKTTGFCAYRKSSDGPDVAFRVSKEAQLSAPTNQLFSDVAFPEDFSLLATVKPKKGSHSYLLSIYNEQGTLQIGLTLGWEPMFVYEDHQGHPPPEDYPIFRNINLSDGKWHRVAFSVHKKTITLFLDCVKKATLPLKRSDGPIIDTNGIVVFGTRILDEQVFEGDIQQLMIVPDARAAHDYCEHYTPDCDTAIPDAPQSQDPNQDEYYPEAAEAEGEGYYYEYPYYEDEKTETATTKPTPEKAEVLRPETEIEEEETPPPTEAPAIPVTDAPRFETREESRVESRVESRVDNPTTDDYNYETVQEEYYTASPYEDVNYSEGEETGIETGTTGEGLIEGEGEILTKTVITSSSSNGSSVISSGSSKKEGSTDGDFTEVTIQEYDQGKDTYYDIYDDKLLTDTSDGIGGQPAEQDQFGISGGARGEKGQKGEPAIIEPGMLIEGPPGPEGPAGYPGPPGTSGPPGPAGDPGERGPPGRAGLPGADGLPGPPGTMLMLPFRFSGGGDGGSKGPQVSAQESQAQAILQQARLALRGPSGPMGLTGRPGPLGSPGPSGLKGESGDLGPQGPRGPQGSPGPLGKPGRRGRAGSDGARGMPGQTGPKGDRGFDGLGGLPGEKGHRGEPGPQGPPGPHGEDGERGDDGDVGPRGLPGEPGPRGLLGPKGPPGPPGITGVPGMDGQVGPKGSVGPQGEPGPPGQQGNPGAQGLPGPQGAIGPPGEKGPLGKSGLPGMPGADGPPGHPGKEGPSGEKGTLGPAGPQGPIGYPGPRGVKGADGIRGLKGTKGEKGEDGFPGFKGDMGTKGDRGEIGPPGPRGEDGPEGPKGRGGPSGEIGPLGPGGEKGKLGVPGLPGYPGRQGPKGSIGFPGFAGANGEKGTRGTPGKPGPRGQRGPTGPRGERGPRGATGKPGPKGNSGGDGPAGPPGERGPPGPQGPTGFAGPKGPPGPPGKDGLPGHPGQRGETGFQGKTGPPGPPGVVGPQGPTGETGPMGERGHPGPPGPPGEQGLPGVAGKEGSKGDPGPAGLPGKDGPPGLRGFTGERGLPGPIGVPGLKGNEGPPGPPGPAGSPGERGAAGPAGPIGLPGRPGPQGPPGPAGEKGTPGEKGPQGPAGRDGIQGPVGLPGPAGPVGPPGEDGDKGEIGEPGQKGNRGDKGEQGPPGPPGPQGPIGQPGPSGSDGEPGPRGQQGLFGQKGDEGPRGFAGPPGPVGLQGLPGPPGEKGETGDVGQMGPPGPPGPRGPSGSPGADGPQGPPGGIGNPGPVGEKGDPGESGEPGLPGELGPPGPKGERGEKGEAGLQGTSGPPGPKGPPGDDGPKGSPGPVGFPGDPGPPGESGPAGQDGPPGDKGEDGESGQPGSPGPTGEPGPSGPPGKRGPPGPTGPEGRQGEKGAKGESGLEGPPGKTGPIGPQGPPGKPGPEGLRGIPGPVGEQGLPGSPGPDGPPGPMGPPGIPGLKGDSGPKGEKGHPGLIGLIGPPGEQGEKGDRGLPGPQGPHGAKGEQGILGPSGPVGPPGPPGLPGGPGPKGAKGSSGPTGPKGESGIPGPPGPPGPPGEVIQPLPIHSSKRTRRSIDASQVVDDGNADNYMDYADGMEEIFGSLNSLKQEIEQMKHPLGNQQNPARTCKDLLLCHPELPDGEYWIDPNQGCSRDSFKVFCNFTAGGESCIFPDKKSEGNKLARWPKELPGTWYSHYKRGSMLSYVDSNGNPIGVVQMTFMKLLSASVKQNITYNCYQSVAWHDATTNSYDKAIRFLGSNDEEMSYDNNPSIRAIMDGCASKKGYQKTVLELDTSKVEQAPIVDIMFNDFGDPVQKFGFEVGPVCFMG

Target ORF information:

RefSeq Version	XM_012969175.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen, type V, alpha 1 (col5a1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012969175.2

ORF Insert Sequence:

ATGAGTATGG ATTCCCACAC ACGATGGAAA AGACGGAATT GGACGACAGA GTGGCCCCTC 
CTTGTAGCTT TAGCCTTGTC TATATGTTCC CGGTTCAGCG CAGCTGAACC GGCGGACCTG 
TTGAAGGCCT TAGAATTTCA CAATTTACCA GACGGTATCA AAAAAACGAC GGGCTTCTGC 
GCCTACCGCA AATCTTCAGA TGGACCAGAC GTGGCATTTA GAGTGTCCAA AGAGGCCCAG 
CTTAGCGCTC CGACCAATCA ATTGTTCTCC GATGTCGCAT TCCCAGAAGA TTTCTCCTTG 
TTGGCAACTG TGAAACCCAA AAAAGGAAGC CATTCCTACC TGCTCTCCAT CTACAATGAG 
CAAGGAACCC TGCAGATCGG CCTGACGCTG GGCTGGGAAC CGATGTTCGT CTATGAAGAC 
CACCAGGGAC ACCCACCCCC CGAGGATTAC CCCATCTTTA GAAACATTAA TCTGTCAGAT 
GGGAAGTGGC ACCGGGTAGC TTTCAGCGTG CACAAGAAGA CGATCACCCT GTTCCTGGAC 
TGCGTCAAGA AGGCCACCCT GCCCTTGAAA AGAAGCGACG GCCCCATTAT AGACACGAAC 
GGCATCGTCG TGTTCGGCAC CAGGATCCTG GACGAGCAAG TCTTTGAGGG AGACATTCAA 
CAGCTGATGA TCGTGCCCGA CGCCCGTGCT GCACATGATT ATTGTGAACA CTACACCCCC 
GACTGTGACA CCGCCATTCC AGATGCACCG CAGTCTCAGG ATCCCAACCA AGATGAATAT 
TACCCAGAAG CTGCTGAAGC CGAAGGCGAG GGTTATTACT ATGAATATCC TTACTACGAA 
GATGAGAAGA CAGAAACTGC AACTACCAAG CCCACGCCTG AGAAAGCCGA GGTACTAAGG 
CCCGAGACAG AAATCGAAGA GGAGGAAACA CCCCCACCAA CTGAAGCCCC TGCTATTCCC 
GTCACTGATG CTCCACGATT TGAGACCAGG GAAGAGAGCA GGGTAGAGAG CAGGGTAGAG 
AGCAGAGTGG ATAACCCCAC CACCGATGAC TACAACTATG AAACCGTACA GGAAGAATAT 
TACACCGCCT CTCCTTATGA AGATGTCAAC TACTCTGAAG GAGAGGAAAC AGGAATTGAG 
ACTGGGACTA CTGGTGAAGG CCTCATCGAG GGAGAAGGAG AAATTTTGAC CAAAACTGTC 
ATCACAAGCA GCTCATCCAA TGGCTCATCG GTCATCAGCA GTGGAAGTTC CAAAAAAGAA 
GGCAGTACCG ACGGAGATTT CACAGAAGTG ACCATACAGG AGTACGACCA AGGGAAAGAT 
ACTTACTATG ACATCTACGA TGATAAGCTT CTCACTGACA CTTCAGACGG AATTGGTGGG 
CAGCCAGCTG AACAAGATCA ATTTGGCATT TCAGGTGGGG CCAGAGGTGA GAAAGGACAG 
AAGGGAGAGC CAGCCATCAT TGAACCCGGA ATGCTGATCG AAGGACCACC AGGCCCAGAA 
GGTCCAGCTG GTTATCCCGG TCCTCCTGGT ACCTCAGGTC CTCCTGGTCC AGCTGGAGAT 
CCTGGAGAGA GGGGCCCCCC TGGTCGTGCA GGTCTTCCTG GAGCTGATGG CTTGCCCGGA 
CCACCAGGAA CCATGCTGAT GTTGCCCTTC CGCTTCTCTG GTGGTGGAGA TGGTGGATCT 
AAAGGACCCC AAGTGTCTGC CCAGGAATCA CAAGCCCAAG CTATTCTCCA ACAAGCAAGA 
CTGGCGTTGA GAGGGCCATC TGGTCCGATG GGTCTTACTG GAAGACCAGG CCCATTGGGT 
TCTCCAGGTC CTTCAGGATT AAAGGGAGAG TCAGGAGATT TGGGACCTCA GGGGCCACGT 
GGGCCACAAG GTTCTCCCGG TCCATTAGGA AAACCAGGAC GAAGGGGACG AGCTGGTAGT 
GATGGTGCTC GGGGAATGCC TGGACAAACA GGACCAAAGG GTGACCGAGG CTTTGACGGG 
TTGGGCGGTT TGCCAGGAGA AAAGGGACAT CGTGGTGAGC CTGGTCCACA AGGTCCACCA 
GGGCCACATG GAGAAGACGG AGAAAGGGGT GATGATGGAG ACGTTGGACC TAGAGGGTTA 
CCAGGTGAAC CTGGACCCCG AGGGTTGCTT GGACCTAAAG GACCTCCTGG CCCACCGGGA 
ATCACCGGTG TTCCTGGAAT GGACGGTCAA GTTGGACCCA AAGGTAGTGT GGGACCTCAA 
GGTGAGCCAG GACCACCTGG GCAGCAAGGA AACCCAGGAG CACAGGGTCT TCCAGGTCCT 
CAGGGAGCTA TTGGACCACC AGGAGAGAAG GGACCACTGG GAAAATCAGG TCTTCCCGGA 
ATGCCCGGAG CTGATGGACC TCCCGGTCAC CCCGGGAAAG AAGGACCCTC AGGAGAGAAA 
GGAACCCTGG GTCCTGCAGG GCCACAGGGA CCAATTGGAT ACCCAGGACC ACGTGGTGTT 
AAGGGAGCTG ATGGAATTCG TGGTCTAAAG GGAACAAAAG GAGAAAAGGG TGAAGATGGA 
TTCCCTGGAT TTAAGGGTGA TATGGGCACC AAAGGAGACA GAGGTGAAAT CGGTCCTCCT 
GGTCCTAGAG GTGAAGATGG TCCTGAAGGT CCAAAGGGTC GAGGTGGTCC ATCTGGAGAA 
ATAGGTCCAC TTGGTCCTGG CGGTGAAAAG GGAAAGCTTG GTGTTCCTGG TCTACCAGGT 
TATCCAGGAA GACAAGGTCC AAAGGGTTCT ATCGGATTCC CAGGATTCGC AGGAGCAAAC 
GGAGAGAAAG GAACAAGGGG TACCCCTGGC AAACCGGGTC CAAGGGGGCA ACGAGGTCCA 
ACGGGTCCAC GTGGTGAAAG AGGTCCAAGG GGAGCTACAG GCAAGCCAGG CCCCAAGGGT 
AACTCCGGAG GGGATGGCCC AGCAGGTCCA CCTGGTGAAA GGGGACCACC TGGACCTCAA 
GGACCCACTG GATTTGCAGG ACCGAAGGGC CCCCCTGGTC CACCAGGGAA AGATGGACTT 
CCCGGCCACC CTGGACAGAG AGGAGAGACT GGATTCCAAG GAAAGACTGG CCCTCCTGGT 
CCCCCTGGCG TTGTCGGTCC TCAGGGTCCA ACTGGAGAGA CTGGCCCAAT GGGTGAAAGA 
GGACATCCTG GTCCTCCAGG TCCTCCTGGT GAGCAAGGTT TACCTGGTGT GGCTGGAAAA 
GAAGGATCCA AGGGAGACCC CGGTCCTGCT GGTTTGCCTG GAAAAGATGG ACCTCCAGGT 
CTAAGAGGTT TTACTGGAGA AAGAGGTCTC CCCGGTCCGA TTGGTGTTCC TGGACTGAAA 
GGCAATGAGG GTCCTCCTGG TCCACCTGGC CCAGCTGGTT CCCCCGGTGA AAGAGGAGCT 
GCGGGTCCAG CCGGACCTAT TGGACTGCCT GGAAGACCTG GCCCTCAGGG GCCACCAGGA 
CCTGCCGGCG AGAAGGGTAC ACCAGGTGAG AAAGGTCCCC AGGGACCTGC TGGACGTGAT 
GGTATCCAAG GCCCCGTTGG ACTTCCAGGC CCAGCTGGAC CTGTAGGGCC ACCTGGTGAA 
GATGGTGATA AGGGTGAAAT CGGAGAGCCT GGGCAAAAAG GAAACAGAGG AGATAAAGGA 
GAGCAGGGTC CCCCAGGTCC ACCTGGCCCA CAAGGTCCCA TTGGTCAGCC TGGCCCATCA 
GGATCTGATG GAGAGCCAGG CCCCAGGGGT CAACAAGGTC TTTTTGGACA AAAAGGTGAT 
GAAGGACCTA GAGGTTTTGC TGGACCACCA GGACCTGTTG GACTGCAGGG TTTACCTGGA 
CCACCTGGTG AGAAGGGAGA AACCGGAGAC GTTGGTCAGA TGGGTCCTCC TGGTCCCCCT 
GGTCCTAGAG GTCCTTCTGG CTCCCCTGGA GCTGATGGCC CACAAGGTCC TCCTGGAGGA 
ATTGGCAACC CTGGTCCTGT AGGAGAGAAG GGTGACCCTG GAGAGTCTGG GGAACCTGGT 
CTTCCTGGTG AACTTGGACC ACCGGGCCCT AAAGGTGAAA GAGGTGAAAA GGGAGAAGCG 
GGTCTTCAAG GAACTTCTGG CCCTCCAGGT CCTAAAGGTC CTCCAGGTGA TGATGGTCCA 
AAAGGTAGCC CTGGCCCAGT TGGATTTCCT GGTGATCCTG GTCCTCCTGG AGAATCTGGT 
CCTGCAGGTC AAGATGGTCC ACCTGGTGAT AAGGGAGAGG ACGGTGAATC TGGACAGCCA 
GGGTCACCAG GTCCTACTGG AGAGCCTGGG CCTTCAGGAC CACCTGGAAA GAGGGGTCCT 
CCTGGTCCAA CAGGTCCTGA AGGACGTCAA GGAGAAAAAG GAGCAAAGGG TGAATCAGGT 
TTGGAAGGAC CTCCTGGTAA GACTGGTCCT ATTGGTCCCC AAGGACCTCC CGGAAAACCT 
GGACCTGAAG GCCTTCGAGG AATTCCAGGC CCTGTTGGTG AACAAGGTCT CCCTGGTTCA 
CCTGGACCTG ATGGCCCTCC CGGACCTATG GGTCCACCAG GTATACCAGG TCTGAAAGGA 
GACTCAGGTC CAAAAGGAGA AAAGGGTCAT CCAGGCTTGA TAGGTCTCAT TGGACCTCCT 
GGTGAACAGG GAGAAAAGGG AGACCGTGGT TTGCCAGGCC CCCAGGGACC ACATGGTGCT 
AAAGGAGAAC AGGGTATTCT TGGTCCATCT GGTCCTGTTG GTCCACCTGG TCCTCCAGGC 
TTACCGGGTG GTCCTGGTCC AAAAGGTGCT AAAGGTTCAT CGGGTCCAAC TGGTCCAAAG 
GGTGAATCTG GAATCCCTGG TCCACCAGGT CCTCCAGGAC CTCCAGGCGA GGTGATCCAG 
CCTCTGCCTA TCCATTCCTC AAAGAGGACA AGACGTAGCA TTGATGCAAG TCAAGTAGTG 
GACGATGGAA ATGCAGACAA CTACATGGAC TATGCCGATG GAATGGAGGA AATATTTGGT 
TCTCTCAACT CCCTAAAACA GGAAATTGAA CAAATGAAAC ACCCACTGGG CAATCAGCAG 
AACCCAGCAA GGACTTGCAA GGACCTGCTT CTGTGCCATC CTGAACTCCC AGATGGGGAA 
TATTGGATTG ACCCAAATCA AGGATGCTCT AGAGACTCGT TCAAGGTTTT CTGTAATTTC 
ACAGCCGGTG GAGAATCTTG TATCTTCCCC GACAAGAAGT CTGAAGGAAA TAAACTGGCT 
CGTTGGCCCA AAGAGCTGCC TGGTACATGG TATAGTCATT ACAAGCGGGG TTCCATGTTG 
TCCTACGTTG ATTCAAATGG CAACCCCATT GGAGTGGTGC AGATGACCTT CATGAAGCTT 
CTGAGTGCTT CGGTGAAACA GAATATCACC TACAACTGCT ACCAGTCCGT GGCATGGCAC 
GACGCCACCA CCAACAGCTA CGACAAGGCC ATTCGCTTCC TGGGGTCCAA CGACGAAGAA 
ATGTCCTACG ACAACAATCC TTCCATTCGA GCCATTATGG ATGGATGTGC GTCAAAGAAA 
GGTTATCAGA AGACAGTTCT GGAACTCGAC ACGTCCAAAG TGGAACAGGC CCCCATTGTG 
GATATTATGT TCAACGACTT CGGAGATCCA GTACAAAAAT TCGGCTTTGA GGTGGGACCA 
GTGTGTTTCA TGGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col5a1 ( XM_012969176.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824630.1 Xenopus tropicalis collagen, type V, alpha 1 (col5a1), transcript variant X3, mRNA.

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CloneID	OXa30188
Clone ID Related Accession (Same CDS sequence)	XM_012969176.2 , XM_012969176.3
Accession Version	XM_012969176.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-1(V) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683374.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012969176.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTATGG ATTCCCACAC ACGATGGAAA AGACGGAATT GGACGACAGA GTGGCCCCTC 
CTTGTAGCTT TAGCCTTGTC TATATGTTCC CGGTTCAGCG CAGCTGAACC GGCGGACCTG 
TTGAAGGCCT TAGAATTTCA CAATTTACCA GACGGTATCA AAAAAACGAC GGGCTTCTGC 
GCCTACCGCA AATCTTCAGA TGGACCAGAC GTGGCATTTA GAGTGTCCAA AGAGGCCCAG 
CTTAGCGCTC CGACCAATCA ATTGTTCTCC GATGTCGCAT TCCCAGAAGA TTTCTCCTTG 
TTGGCAACTG TGAAACCCAA AAAAGGAAGC CATTCCTACC TGCTCTCCAT CTACAATGAG 
CAAGGAACCC TGCAGATCGG CCTGACGCTG GGCTGGGAAC CGATGTTCGT CTATGAAGAC 
CACCAGGGAC ACCCACCCCC CGAGGATTAC CCCATCTTTA GAAACATTAA TCTGTCAGAT 
GGGAAGTGGC ACCGGGTAGC TTTCAGCGTG CACAAGAAGA CGATCACCCT GTTCCTGGAC 
TGCGTCAAGA AGGCCACCCT GCCCTTGAAA AGAAGCGACG GCCCCATTAT AGACACGAAC 
GGCATCGTCG TGTTCGGCAC CAGGATCCTG GACGAGCAAG TCTTTGAGGG AGACATTCAA 
CAGCTGATGA TCGTGCCCGA CGCCCGTGCT GCACATGATT ATTGTGAACA CTACACCCCC 
GACTGTGACA CCGCCATTCC AGATGCACCG CAGTCTCAGG ATCCCAACCA AGATGAATAT 
TACCCAGAAG CTGCTGAAGC CGAAGGCGAG GGTTATTACT ATGAATATCC TTACTACGAA 
GATGAGAAGA CAGAAACTGC AACTACCAAG CCCACGCCTG AGAAAGCCGA GGTACTAAGG 
CCCGAGACAG AAATCGAAGA GGAGGAAACA CCCCCACCAA CTGAAGCCCC TGCTATTCCC 
GTCACTGATG CTCCACGATT TGAGACCAGG GAAGAGAGCA GGGTAGAGAG CAGGGTAGAG 
AGCAGAGTGG ATAACCCCAC CACCGATGAC TACAACTATG AAACCGTACA GGAAGAATAT 
TACACCGCCT CTCCTTATGA AGATGTCAAC TACTCTGAAG GAGAGGAAAC AGGAATTGAG 
ACTGGGACTA CTGGTGAAGG CCTCATCGAG GGAGAAGGAG AAATTTTGAC CAAAACTGTC 
ATCACAAGCA GCTCATCCAA TGGCTCATCG GTCATCAGCA GTGGAAGTTC CAAAAAAGAA 
GGCAGTACCG ACGGAGATTT CACAGAAGTG ACCATACAGG AGTACGACCA AGGGAAAGAT 
ACTTACTATG ACATCTACGA TGATAAGCTT CTCACTGACA CTTCAGACGG AATTGGTGGG 
CAGCCAGCTG AACAAGATCA ATTTGGCATT TCAGGTGGGG CCAGAGGTGA GAAAGGACAG 
AAGGGAGAGC CAGCCATCAT TGAACCCGGA ATGCTGATCG AAGGACCACC AGGCCCAGAA 
GGTCCAGCTG GTTATCCCGG TCCTCCTGGT ACCTCAGGTC CTCCTGGTCC AGCTGGAGAT 
CCTGGAGAGA GGGGCCCCCC TGGTCGTGCA GGTCTTCCTG GAGCTGATGG CTTGCCCGGA 
CCACCAGGAA CCATGCTGAT GTTGCCCTTC CGCTTCTCTG GTGGTGGAGA TGGTGGATCT 
AAAGGACCCC AAGTGTCTGC CCAGGAATCA CAAGCCCAAG CTATTCTCCA ACAAGCAAGA 
CTGGCGTTGA GAGGGCCATC TGGTCCGATG GGTCTTACTG GAAGACCAGG CCCATTGGGT 
TCTCCAGGTC CTTCAGGATT AAAGGGAGAG TCAGGAGATT TGGGACCTCA GGGGCCACGT 
GGGCCACAAG GTTCTCCCGG TCCATTAGGA AAACCAGGAC GAAGGGGACG AGCTGGTAGT 
GATGGTGCTC GGGGAATGCC TGGACAAACA GGACCAAAGG GTGACCGAGG CTTTGACGGG 
TTGGGCGGTT TGCCAGGAGA AAAGGGACAT CGTGGTGAGC CTGGTCCACA AGGTCCACCA 
GGGCCACATG GAGAAGACGG AGAAAGGGGT GATGATGGAG ACGTTGGACC TAGAGGGTTA 
CCAGGTGAAC CTGGACCCCG AGGGTTGCTT GGACCTAAAG GACCTCCTGG CCCACCGGGA 
ATCACCGGTG TTCCTGGAAT GGACGGTCAA GTTGGACCCA AAGGTAGTGT GGGACCTCAA 
GGTGAGCCAG GACCACCTGG GCAGCAAGGA AACCCAGGAG CACAGGGTCT TCCAGGTCCT 
CAGGGAGCTA TTGGACCACC AGGAGAGAAG GGACCACTGG GAAAATCAGG TCTTCCCGGA 
ATGCCCGGAG CTGATGGACC TCCCGGTCAC CCCGGGAAAG AAGGACCCTC AGGAGAGAAA 
GGAACCCTGG GTCCTGCAGG GCCACAGGGA CCAATTGGAT ACCCAGGACC ACGTGGTGTT 
AAGGGAGCTG ATGGAATTCG TGGTCTAAAG GGAACAAAAG GAGAAAAGGG TGAAGATGGA 
TTCCCTGGAT TTAAGGGTGA TATGGGCACC AAAGGAGACA GAGGTGAAAT CGGTCCTCCT 
GGTCCTAGAG GTGAAGATGG TCCTGAAGGT CCAAAGGGTC GAGGTGGTCC ATCTGGAGAA 
ATAGGTCCAC TTGGTCCTGG CGGTGAAAAG GGAAAGCTTG GTGTTCCTGG TCTACCAGGT 
TATCCAGGAA GACAAGGTCC AAAGGGTTCT ATCGGATTCC CAGGATTCGC AGGAGCAAAC 
GGAGAGAAAG GAACAAGGGG TACCCCTGGC AAACCGGGTC CAAGGGGGCA ACGAGGTCCA 
ACGGGTCCAC GTGGTGAAAG AGGTCCAAGG GGAGCTACAG GCAAGCCAGG CCCCAAGGGT 
AACTCCGGAG GGGATGGCCC AGCAGGTCCA CCTGGTGAAA GGGGACCACC TGGACCTCAA 
GGACCCACTG GATTTGCAGG ACCGAAGGGC CCCCCTGGTC CACCAGGGAA AGATGGACTT 
CCCGGCCACC CTGGACAGAG AGGAGAGACT GGATTCCAAG GAAAGACTGG CCCTCCTGGT 
CCCCCTGGCG TTGTCGGTCC TCAGGGTCCA ACTGGAGAGA CTGGCCCAAT GGGTGAAAGA 
GGACATCCTG GTCCTCCAGG TCCTCCTGGT GAGCAAGGTT TACCTGGTGT GGCTGGAAAA 
GAAGGATCCA AGGGAGACCC CGGTCCTGCT GGTTTGCCTG GAAAAGATGG ACCTCCAGGT 
CTAAGAGGTT TTACTGGAGA AAGAGGTCTC CCCGGTCCGA TTGGTGTTCC TGGACTGAAA 
GGCAATGAGG GTCCTCCTGG TCCACCTGGC CCAGCTGGTT CCCCCGGTGA AAGAGGAGCT 
GCGGGTCCAG CCGGACCTAT TGGACTGCCT GGAAGACCTG GCCCTCAGGG GCCACCAGGA 
CCTGCCGGCG AGAAGGGTAC ACCAGGTGAG AAAGGTCCCC AGGGACCTGC TGGACGTGAT 
GGTATCCAAG GCCCCGTTGG ACTTCCAGGC CCAGCTGGAC CTGTAGGGCC ACCTGGTGAA 
GATGGTGATA AGGGTGAAAT CGGAGAGCCT GGGCAAAAAG GAAACAGAGG AGATAAAGGA 
GAGCAGGGTC CCCCAGGTCC ACCTGGCCCA CAAGGTCCCA TTGGTCAGCC TGGCCCATCA 
GGATCTGATG GAGAGCCAGG CCCCAGGGGT CAACAAGGTC TTTTTGGACA AAAAGGTGAT 
GAAGGACCTA GAGGTTTTGC TGGACCACCA GGACCTGTTG GACTGCAGGG TTTACCTGGA 
CCACCTGGTG AGAAGGGAGA AACCGGAGAC GTTGGTCAGA TGGGTCCTCC TGGTCCCCCT 
GGTCCTAGAG GTCCTTCTGG CTCCCCTGGA GCTGATGGCC CACAAGGTCC TCCTGGAGGA 
ATTGGCAACC CTGGTCCTGT AGGAGAGAAG GGTGACCCTG GAGAGTCTGG GGAACCTGGT 
CTTCCTGGTG AACTTGGACC ACCGGGCCCT AAAGGTGAAA GAGGTGAAAA GGGAGAAGCG 
GGTCTTCAAG GAACTTCTGG CCCTCCAGGT CCTAAAGGTC CTCCAGGTGA TGATGGTCCA 
AAAGGTAGCC CTGGCCCAGT TGGATTTCCT GGTGATCCTG GTCCTCCTGG AGAATCTGGT 
CCTGCAGGTC AAGATGGTCC ACCTGGTGAT AAGGGAGAGG ACGGTGAATC TGGACAGCCA 
GGGTCACCAG GTCCTACTGG AGAGCCTGGG CCTTCAGGAC CACCTGGAAA GAGGGGTCCT 
CCTGGTCCAA CAGGTCCTGA AGGACGTCAA GGAGAAAAAG GAGCAAAGGG TGAATCAGGT 
TTGGAAGGAC CTCCTGGTAA GACTGGTCCT ATTGGTCCCC AAGGACCTCC CGGAAAACCT 
GGACCTGAAG GCCTTCGAGG AATTCCAGGC CCTGTTGGTG AACAAGGTCT CCCTGGTTCA 
CCTGGACCTG ATGGCCCTCC CGGACCTATG GGTCCACCAG GTATACCAGG TCTGAAAGGA 
GACTCAGGTC CAAAAGGAGA AAAGGGTCAT CCAGGCTTGA TAGGTCTCAT TGGACCTCCT 
GGTGAAACTA AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824630.1
CDS	343..4917
Translation	MSMDSHTRWKRRNWTTEWPLLVALALSICSRFSAAEPADLLKALEFHNLPDGIKKTTGFCAYRKSSDGPDVAFRVSKEAQLSAPTNQLFSDVAFPEDFSLLATVKPKKGSHSYLLSIYNEQGTLQIGLTLGWEPMFVYEDHQGHPPPEDYPIFRNINLSDGKWHRVAFSVHKKTITLFLDCVKKATLPLKRSDGPIIDTNGIVVFGTRILDEQVFEGDIQQLMIVPDARAAHDYCEHYTPDCDTAIPDAPQSQDPNQDEYYPEAAEAEGEGYYYEYPYYEDEKTETATTKPTPEKAEVLRPETEIEEEETPPPTEAPAIPVTDAPRFETREESRVESRVESRVDNPTTDDYNYETVQEEYYTASPYEDVNYSEGEETGIETGTTGEGLIEGEGEILTKTVITSSSSNGSSVISSGSSKKEGSTDGDFTEVTIQEYDQGKDTYYDIYDDKLLTDTSDGIGGQPAEQDQFGISGGARGEKGQKGEPAIIEPGMLIEGPPGPEGPAGYPGPPGTSGPPGPAGDPGERGPPGRAGLPGADGLPGPPGTMLMLPFRFSGGGDGGSKGPQVSAQESQAQAILQQARLALRGPSGPMGLTGRPGPLGSPGPSGLKGESGDLGPQGPRGPQGSPGPLGKPGRRGRAGSDGARGMPGQTGPKGDRGFDGLGGLPGEKGHRGEPGPQGPPGPHGEDGERGDDGDVGPRGLPGEPGPRGLLGPKGPPGPPGITGVPGMDGQVGPKGSVGPQGEPGPPGQQGNPGAQGLPGPQGAIGPPGEKGPLGKSGLPGMPGADGPPGHPGKEGPSGEKGTLGPAGPQGPIGYPGPRGVKGADGIRGLKGTKGEKGEDGFPGFKGDMGTKGDRGEIGPPGPRGEDGPEGPKGRGGPSGEIGPLGPGGEKGKLGVPGLPGYPGRQGPKGSIGFPGFAGANGEKGTRGTPGKPGPRGQRGPTGPRGERGPRGATGKPGPKGNSGGDGPAGPPGERGPPGPQGPTGFAGPKGPPGPPGKDGLPGHPGQRGETGFQGKTGPPGPPGVVGPQGPTGETGPMGERGHPGPPGPPGEQGLPGVAGKEGSKGDPGPAGLPGKDGPPGLRGFTGERGLPGPIGVPGLKGNEGPPGPPGPAGSPGERGAAGPAGPIGLPGRPGPQGPPGPAGEKGTPGEKGPQGPAGRDGIQGPVGLPGPAGPVGPPGEDGDKGEIGEPGQKGNRGDKGEQGPPGPPGPQGPIGQPGPSGSDGEPGPRGQQGLFGQKGDEGPRGFAGPPGPVGLQGLPGPPGEKGETGDVGQMGPPGPPGPRGPSGSPGADGPQGPPGGIGNPGPVGEKGDPGESGEPGLPGELGPPGPKGERGEKGEAGLQGTSGPPGPKGPPGDDGPKGSPGPVGFPGDPGPPGESGPAGQDGPPGDKGEDGESGQPGSPGPTGEPGPSGPPGKRGPPGPTGPEGRQGEKGAKGESGLEGPPGKTGPIGPQGPPGKPGPEGLRGIPGPVGEQGLPGSPGPDGPPGPMGPPGIPGLKGDSGPKGEKGHPGLIGLIGPPGETK

Target ORF information:

RefSeq Version	XM_012969176.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen, type V, alpha 1 (col5a1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012969176.2

ORF Insert Sequence:

ATGAGTATGG ATTCCCACAC ACGATGGAAA AGACGGAATT GGACGACAGA GTGGCCCCTC 
CTTGTAGCTT TAGCCTTGTC TATATGTTCC CGGTTCAGCG CAGCTGAACC GGCGGACCTG 
TTGAAGGCCT TAGAATTTCA CAATTTACCA GACGGTATCA AAAAAACGAC GGGCTTCTGC 
GCCTACCGCA AATCTTCAGA TGGACCAGAC GTGGCATTTA GAGTGTCCAA AGAGGCCCAG 
CTTAGCGCTC CGACCAATCA ATTGTTCTCC GATGTCGCAT TCCCAGAAGA TTTCTCCTTG 
TTGGCAACTG TGAAACCCAA AAAAGGAAGC CATTCCTACC TGCTCTCCAT CTACAATGAG 
CAAGGAACCC TGCAGATCGG CCTGACGCTG GGCTGGGAAC CGATGTTCGT CTATGAAGAC 
CACCAGGGAC ACCCACCCCC CGAGGATTAC CCCATCTTTA GAAACATTAA TCTGTCAGAT 
GGGAAGTGGC ACCGGGTAGC TTTCAGCGTG CACAAGAAGA CGATCACCCT GTTCCTGGAC 
TGCGTCAAGA AGGCCACCCT GCCCTTGAAA AGAAGCGACG GCCCCATTAT AGACACGAAC 
GGCATCGTCG TGTTCGGCAC CAGGATCCTG GACGAGCAAG TCTTTGAGGG AGACATTCAA 
CAGCTGATGA TCGTGCCCGA CGCCCGTGCT GCACATGATT ATTGTGAACA CTACACCCCC 
GACTGTGACA CCGCCATTCC AGATGCACCG CAGTCTCAGG ATCCCAACCA AGATGAATAT 
TACCCAGAAG CTGCTGAAGC CGAAGGCGAG GGTTATTACT ATGAATATCC TTACTACGAA 
GATGAGAAGA CAGAAACTGC AACTACCAAG CCCACGCCTG AGAAAGCCGA GGTACTAAGG 
CCCGAGACAG AAATCGAAGA GGAGGAAACA CCCCCACCAA CTGAAGCCCC TGCTATTCCC 
GTCACTGATG CTCCACGATT TGAGACCAGG GAAGAGAGCA GGGTAGAGAG CAGGGTAGAG 
AGCAGAGTGG ATAACCCCAC CACCGATGAC TACAACTATG AAACCGTACA GGAAGAATAT 
TACACCGCCT CTCCTTATGA AGATGTCAAC TACTCTGAAG GAGAGGAAAC AGGAATTGAG 
ACTGGGACTA CTGGTGAAGG CCTCATCGAG GGAGAAGGAG AAATTTTGAC CAAAACTGTC 
ATCACAAGCA GCTCATCCAA TGGCTCATCG GTCATCAGCA GTGGAAGTTC CAAAAAAGAA 
GGCAGTACCG ACGGAGATTT CACAGAAGTG ACCATACAGG AGTACGACCA AGGGAAAGAT 
ACTTACTATG ACATCTACGA TGATAAGCTT CTCACTGACA CTTCAGACGG AATTGGTGGG 
CAGCCAGCTG AACAAGATCA ATTTGGCATT TCAGGTGGGG CCAGAGGTGA GAAAGGACAG 
AAGGGAGAGC CAGCCATCAT TGAACCCGGA ATGCTGATCG AAGGACCACC AGGCCCAGAA 
GGTCCAGCTG GTTATCCCGG TCCTCCTGGT ACCTCAGGTC CTCCTGGTCC AGCTGGAGAT 
CCTGGAGAGA GGGGCCCCCC TGGTCGTGCA GGTCTTCCTG GAGCTGATGG CTTGCCCGGA 
CCACCAGGAA CCATGCTGAT GTTGCCCTTC CGCTTCTCTG GTGGTGGAGA TGGTGGATCT 
AAAGGACCCC AAGTGTCTGC CCAGGAATCA CAAGCCCAAG CTATTCTCCA ACAAGCAAGA 
CTGGCGTTGA GAGGGCCATC TGGTCCGATG GGTCTTACTG GAAGACCAGG CCCATTGGGT 
TCTCCAGGTC CTTCAGGATT AAAGGGAGAG TCAGGAGATT TGGGACCTCA GGGGCCACGT 
GGGCCACAAG GTTCTCCCGG TCCATTAGGA AAACCAGGAC GAAGGGGACG AGCTGGTAGT 
GATGGTGCTC GGGGAATGCC TGGACAAACA GGACCAAAGG GTGACCGAGG CTTTGACGGG 
TTGGGCGGTT TGCCAGGAGA AAAGGGACAT CGTGGTGAGC CTGGTCCACA AGGTCCACCA 
GGGCCACATG GAGAAGACGG AGAAAGGGGT GATGATGGAG ACGTTGGACC TAGAGGGTTA 
CCAGGTGAAC CTGGACCCCG AGGGTTGCTT GGACCTAAAG GACCTCCTGG CCCACCGGGA 
ATCACCGGTG TTCCTGGAAT GGACGGTCAA GTTGGACCCA AAGGTAGTGT GGGACCTCAA 
GGTGAGCCAG GACCACCTGG GCAGCAAGGA AACCCAGGAG CACAGGGTCT TCCAGGTCCT 
CAGGGAGCTA TTGGACCACC AGGAGAGAAG GGACCACTGG GAAAATCAGG TCTTCCCGGA 
ATGCCCGGAG CTGATGGACC TCCCGGTCAC CCCGGGAAAG AAGGACCCTC AGGAGAGAAA 
GGAACCCTGG GTCCTGCAGG GCCACAGGGA CCAATTGGAT ACCCAGGACC ACGTGGTGTT 
AAGGGAGCTG ATGGAATTCG TGGTCTAAAG GGAACAAAAG GAGAAAAGGG TGAAGATGGA 
TTCCCTGGAT TTAAGGGTGA TATGGGCACC AAAGGAGACA GAGGTGAAAT CGGTCCTCCT 
GGTCCTAGAG GTGAAGATGG TCCTGAAGGT CCAAAGGGTC GAGGTGGTCC ATCTGGAGAA 
ATAGGTCCAC TTGGTCCTGG CGGTGAAAAG GGAAAGCTTG GTGTTCCTGG TCTACCAGGT 
TATCCAGGAA GACAAGGTCC AAAGGGTTCT ATCGGATTCC CAGGATTCGC AGGAGCAAAC 
GGAGAGAAAG GAACAAGGGG TACCCCTGGC AAACCGGGTC CAAGGGGGCA ACGAGGTCCA 
ACGGGTCCAC GTGGTGAAAG AGGTCCAAGG GGAGCTACAG GCAAGCCAGG CCCCAAGGGT 
AACTCCGGAG GGGATGGCCC AGCAGGTCCA CCTGGTGAAA GGGGACCACC TGGACCTCAA 
GGACCCACTG GATTTGCAGG ACCGAAGGGC CCCCCTGGTC CACCAGGGAA AGATGGACTT 
CCCGGCCACC CTGGACAGAG AGGAGAGACT GGATTCCAAG GAAAGACTGG CCCTCCTGGT 
CCCCCTGGCG TTGTCGGTCC TCAGGGTCCA ACTGGAGAGA CTGGCCCAAT GGGTGAAAGA 
GGACATCCTG GTCCTCCAGG TCCTCCTGGT GAGCAAGGTT TACCTGGTGT GGCTGGAAAA 
GAAGGATCCA AGGGAGACCC CGGTCCTGCT GGTTTGCCTG GAAAAGATGG ACCTCCAGGT 
CTAAGAGGTT TTACTGGAGA AAGAGGTCTC CCCGGTCCGA TTGGTGTTCC TGGACTGAAA 
GGCAATGAGG GTCCTCCTGG TCCACCTGGC CCAGCTGGTT CCCCCGGTGA AAGAGGAGCT 
GCGGGTCCAG CCGGACCTAT TGGACTGCCT GGAAGACCTG GCCCTCAGGG GCCACCAGGA 
CCTGCCGGCG AGAAGGGTAC ACCAGGTGAG AAAGGTCCCC AGGGACCTGC TGGACGTGAT 
GGTATCCAAG GCCCCGTTGG ACTTCCAGGC CCAGCTGGAC CTGTAGGGCC ACCTGGTGAA 
GATGGTGATA AGGGTGAAAT CGGAGAGCCT GGGCAAAAAG GAAACAGAGG AGATAAAGGA 
GAGCAGGGTC CCCCAGGTCC ACCTGGCCCA CAAGGTCCCA TTGGTCAGCC TGGCCCATCA 
GGATCTGATG GAGAGCCAGG CCCCAGGGGT CAACAAGGTC TTTTTGGACA AAAAGGTGAT 
GAAGGACCTA GAGGTTTTGC TGGACCACCA GGACCTGTTG GACTGCAGGG TTTACCTGGA 
CCACCTGGTG AGAAGGGAGA AACCGGAGAC GTTGGTCAGA TGGGTCCTCC TGGTCCCCCT 
GGTCCTAGAG GTCCTTCTGG CTCCCCTGGA GCTGATGGCC CACAAGGTCC TCCTGGAGGA 
ATTGGCAACC CTGGTCCTGT AGGAGAGAAG GGTGACCCTG GAGAGTCTGG GGAACCTGGT 
CTTCCTGGTG AACTTGGACC ACCGGGCCCT AAAGGTGAAA GAGGTGAAAA GGGAGAAGCG 
GGTCTTCAAG GAACTTCTGG CCCTCCAGGT CCTAAAGGTC CTCCAGGTGA TGATGGTCCA 
AAAGGTAGCC CTGGCCCAGT TGGATTTCCT GGTGATCCTG GTCCTCCTGG AGAATCTGGT 
CCTGCAGGTC AAGATGGTCC ACCTGGTGAT AAGGGAGAGG ACGGTGAATC TGGACAGCCA 
GGGTCACCAG GTCCTACTGG AGAGCCTGGG CCTTCAGGAC CACCTGGAAA GAGGGGTCCT 
CCTGGTCCAA CAGGTCCTGA AGGACGTCAA GGAGAAAAAG GAGCAAAGGG TGAATCAGGT 
TTGGAAGGAC CTCCTGGTAA GACTGGTCCT ATTGGTCCCC AAGGACCTCC CGGAAAACCT 
GGACCTGAAG GCCTTCGAGG AATTCCAGGC CCTGTTGGTG AACAAGGTCT CCCTGGTTCA 
CCTGGACCTG ATGGCCCTCC CGGACCTATG GGTCCACCAG GTATACCAGG TCTGAAAGGA 
GACTCAGGTC CAAAAGGAGA AAAGGGTCAT CCAGGCTTGA TAGGTCTCAT TGGACCTCCT 
GGTGAAACTA AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

見積依頼

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電話

03-6811-6572

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03-6811-6573
050-3174-7589(受注専用)

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オンライン注文

col5a1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報