chek1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following chek1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the chek1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa13013 XM_002937515.5
Latest version!
Xenopus tropicalis checkpoint kinase 1 (chek1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OXa13013 XM_004916034.4
Latest version!
Xenopus tropicalis checkpoint kinase 1 (chek1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OXa13013 XM_012966987.3
Latest version!
Xenopus tropicalis checkpoint kinase 1 (chek1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
View Old Accession Versions >>
OXa13013 XM_002937515.4 Xenopus tropicalis checkpoint kinase 1 (chek1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OXa13013 XM_012966987.2 Xenopus tropicalis checkpoint kinase 1 (chek1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OXa13013 XM_004916034.3 Xenopus tropicalis checkpoint kinase 1 (chek1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa13013
    Clone ID Related Accession (Same CDS sequence) XM_002937515.4 , XM_012966987.2 , XM_002937515.5 , XM_012966987.3 , XM_004916034.3 , XM_004916034.4
    Accession Version XM_002937515.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1425bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product serine/threonine-protein kinase Chk1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002937515.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCAGTTC CATTTGTTGA AGACTGGGAT CTTGTCCAGA CTCTTGGGGA AGGGGCATAT 
    GGAGAAGTGC AGCTGGCAGT AAACCGGAAA ACAGAAGAAG CAGTAGCAGT GAAGATTGTG
    GACATGACAC GTGCAGCTGA TTGCCCAGAA AACATCAAAA AGGAGATTTG TATTAATAGG
    ATGCTTAATC ACACAAATAT TGTAAGATTT TATGGACATC GACGGGAAGG AAACATTCAG
    TACCTCTTTC TGGAGTATTG TCGAGGTGGT GAGCTCTTTG ATCGCATAGA ACCTGATGTT
    GGAATGCCTG AGCAAGATGC ACAAAAATTT TTTCAGCAGC TAATTGCTGG TGTGGAATAC
    CTGCACAGCA TTGGAATAAC GCACAGAGAT ATTAAGCCTG AGAACTTACT CTTAGATGAC
    CGAGATCACC TGAAAATCTC TGACTTTGGT TTGGCAACAG TGTTCCGACA CAATGCGAAA
    GAAAGACTTT TAAACAAGAT GTGTGGAACC CTACCCTATG TTGCACCAGA ACTGATTAAG
    TCCAGAGCCT TTAATGCAGA GCCAGTAGAT GTGTGGTCAT GTGGCATTGT ACTGACTGCC
    ATGTTAGCCG GAGAGTTACC ATGGGATCAG CCAACTGAAG CATGCCAGGA GTATTGTGAT
    TGGAAGGAAA AAAATCATTA TCTCACTCCA TGGAAAAAAA TAGGTGCTAC CCCTCTTGCA
    TTGCTGTGTA AAATGTTAAC AGAAAATCCA CAAAGCAGAA TCACCATTCC AGACATAAAG
    AAGGACCGTT GGTTCACAGA ATTAATCAAG AAAGGGATTA AGAGAAGCCG TGCTTTCTCT
    GGAGGTACTT CAGATTCTTC TGGGCTTTGT AAGCAGATCC GATCAGATAT GGAGATTTCA
    ATCATCTCTC ACAGTGAAGA GAAGAATGCA TATTCTAGTA CACAGCCAGA GCCACGGACT
    GCACTGGCAA CATGGGACAG CAATGGTTCT CATATAGACA ATTTGGTGCA GGGAAAAGGC
    ATCAGCTTCT CTCAGCCTGC CTGTCCTGAA AACATGCTTC TGAGCAGCCA GTTAATTGGT
    ACTCCAGGCT CCTCTCAGAA TGTTTGGCAA CGTTTGGTCA AAAGAATGAC CAGGTTCTTT
    ACAAAAGTGG ATGCTGAAAG CTCGTACAGC AATTTGATGG ACACTTGTGA AAAAATGGGA
    TATGTTTTAA AGAAGTCTTG TGCTAACCAG GTCACCCTAT CAACTACTGA CAGAAGAAAT
    AATAAATTAA TATTCAAGGT GAACCTTGTG GAAATGGAAG ACAGGATTCT GTTAGATTTT
    AGACTTTCTA AGGGTGATGG GCTGGAGTTT AAAAGGCATT TCCTGAAGAT TAAGAAGAAG
    ATGGATGCTA TTGTTGCAGT CCAAAAAGTT CTGCCTGCTA CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002937561.2
    CDS289..1713
    Translation

    Target ORF information:

    RefSeq Version XM_002937515.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis checkpoint kinase 1 (chek1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002937515.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCAGTTC CATTTGTTGA AGACTGGGAT CTTGTCCAGA CTCTTGGGGA AGGGGCATAT 
    GGAGAAGTGC AGCTGGCAGT AAACCGGAAA ACAGAAGAAG CAGTAGCAGT GAAGATTGTG
    GACATGACAC GTGCAGCTGA TTGCCCAGAA AACATCAAAA AGGAGATTTG TATTAATAGG
    ATGCTTAATC ACACAAATAT TGTAAGATTT TATGGACATC GACGGGAAGG AAACATTCAG
    TACCTCTTTC TGGAGTATTG TCGAGGTGGT GAGCTCTTTG ATCGCATAGA ACCTGATGTT
    GGAATGCCTG AGCAAGATGC ACAAAAATTT TTTCAGCAGC TAATTGCTGG TGTGGAATAC
    CTGCACAGCA TTGGAATAAC GCACAGAGAT ATTAAGCCTG AGAACTTACT CTTAGATGAC
    CGAGATCACC TGAAAATCTC TGACTTTGGT TTGGCAACAG TGTTCCGACA CAATGCGAAA
    GAAAGACTTT TAAACAAGAT GTGTGGAACC CTACCCTATG TTGCACCAGA ACTGATTAAG
    TCCAGAGCCT TTAATGCAGA GCCAGTAGAT GTGTGGTCAT GTGGCATTGT ACTGACTGCC
    ATGTTAGCCG GAGAGTTACC ATGGGATCAG CCAACTGAAG CATGCCAGGA GTATTGTGAT
    TGGAAGGAAA AAAATCATTA TCTCACTCCA TGGAAAAAAA TAGGTGCTAC CCCTCTTGCA
    TTGCTGTGTA AAATGTTAAC AGAAAATCCA CAAAGCAGAA TCACCATTCC AGACATAAAG
    AAGGACCGTT GGTTCACAGA ATTAATCAAG AAAGGGATTA AGAGAAGCCG TGCTTTCTCT
    GGAGGTACTT CAGATTCTTC TGGGCTTTGT AAGCAGATCC GATCAGATAT GGAGATTTCA
    ATCATCTCTC ACAGTGAAGA GAAGAATGCA TATTCTAGTA CACAGCCAGA GCCACGGACT
    GCACTGGCAA CATGGGACAG CAATGGTTCT CATATAGACA ATTTGGTGCA GGGAAAAGGC
    ATCAGCTTCT CTCAGCCTGC CTGTCCTGAA AACATGCTTC TGAGCAGCCA GTTAATTGGT
    ACTCCAGGCT CCTCTCAGAA TGTTTGGCAA CGTTTGGTCA AAAGAATGAC CAGGTTCTTT
    ACAAAAGTGG ATGCTGAAAG CTCGTACAGC AATTTGATGG ACACTTGTGA AAAAATGGGA
    TATGTTTTAA AGAAGTCTTG TGCTAACCAG GTCACCCTAT CAACTACTGA CAGAAGAAAT
    AATAAATTAA TATTCAAGGT GAACCTTGTG GAAATGGAAG ACAGGATTCT GTTAGATTTT
    AGACTTTCTA AGGGTGATGG GCTGGAGTTT AAAAGGCATT TCCTGAAGAT TAAGAAGAAG
    ATGGATGCTA TTGTTGCAGT CCAAAAAGTT CTGCCTGCTA CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa13013
    Clone ID Related Accession (Same CDS sequence) XM_002937515.4 , XM_012966987.2 , XM_002937515.5 , XM_012966987.3 , XM_004916034.3 , XM_004916034.4
    Accession Version XM_012966987.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1425bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product serine/threonine-protein kinase Chk1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966987.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCAGTTC CATTTGTTGA AGACTGGGAT CTTGTCCAGA CTCTTGGGGA AGGGGCATAT 
    GGAGAAGTGC AGCTGGCAGT AAACCGGAAA ACAGAAGAAG CAGTAGCAGT GAAGATTGTG
    GACATGACAC GTGCAGCTGA TTGCCCAGAA AACATCAAAA AGGAGATTTG TATTAATAGG
    ATGCTTAATC ACACAAATAT TGTAAGATTT TATGGACATC GACGGGAAGG AAACATTCAG
    TACCTCTTTC TGGAGTATTG TCGAGGTGGT GAGCTCTTTG ATCGCATAGA ACCTGATGTT
    GGAATGCCTG AGCAAGATGC ACAAAAATTT TTTCAGCAGC TAATTGCTGG TGTGGAATAC
    CTGCACAGCA TTGGAATAAC GCACAGAGAT ATTAAGCCTG AGAACTTACT CTTAGATGAC
    CGAGATCACC TGAAAATCTC TGACTTTGGT TTGGCAACAG TGTTCCGACA CAATGCGAAA
    GAAAGACTTT TAAACAAGAT GTGTGGAACC CTACCCTATG TTGCACCAGA ACTGATTAAG
    TCCAGAGCCT TTAATGCAGA GCCAGTAGAT GTGTGGTCAT GTGGCATTGT ACTGACTGCC
    ATGTTAGCCG GAGAGTTACC ATGGGATCAG CCAACTGAAG CATGCCAGGA GTATTGTGAT
    TGGAAGGAAA AAAATCATTA TCTCACTCCA TGGAAAAAAA TAGGTGCTAC CCCTCTTGCA
    TTGCTGTGTA AAATGTTAAC AGAAAATCCA CAAAGCAGAA TCACCATTCC AGACATAAAG
    AAGGACCGTT GGTTCACAGA ATTAATCAAG AAAGGGATTA AGAGAAGCCG TGCTTTCTCT
    GGAGGTACTT CAGATTCTTC TGGGCTTTGT AAGCAGATCC GATCAGATAT GGAGATTTCA
    ATCATCTCTC ACAGTGAAGA GAAGAATGCA TATTCTAGTA CACAGCCAGA GCCACGGACT
    GCACTGGCAA CATGGGACAG CAATGGTTCT CATATAGACA ATTTGGTGCA GGGAAAAGGC
    ATCAGCTTCT CTCAGCCTGC CTGTCCTGAA AACATGCTTC TGAGCAGCCA GTTAATTGGT
    ACTCCAGGCT CCTCTCAGAA TGTTTGGCAA CGTTTGGTCA AAAGAATGAC CAGGTTCTTT
    ACAAAAGTGG ATGCTGAAAG CTCGTACAGC AATTTGATGG ACACTTGTGA AAAAATGGGA
    TATGTTTTAA AGAAGTCTTG TGCTAACCAG GTCACCCTAT CAACTACTGA CAGAAGAAAT
    AATAAATTAA TATTCAAGGT GAACCTTGTG GAAATGGAAG ACAGGATTCT GTTAGATTTT
    AGACTTTCTA AGGGTGATGG GCTGGAGTTT AAAAGGCATT TCCTGAAGAT TAAGAAGAAG
    ATGGATGCTA TTGTTGCAGT CCAAAAAGTT CTGCCTGCTA CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012822441.1
    CDS241..1665
    Translation

    Target ORF information:

    RefSeq Version XM_012966987.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis checkpoint kinase 1 (chek1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966987.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCAGTTC CATTTGTTGA AGACTGGGAT CTTGTCCAGA CTCTTGGGGA AGGGGCATAT 
    GGAGAAGTGC AGCTGGCAGT AAACCGGAAA ACAGAAGAAG CAGTAGCAGT GAAGATTGTG
    GACATGACAC GTGCAGCTGA TTGCCCAGAA AACATCAAAA AGGAGATTTG TATTAATAGG
    ATGCTTAATC ACACAAATAT TGTAAGATTT TATGGACATC GACGGGAAGG AAACATTCAG
    TACCTCTTTC TGGAGTATTG TCGAGGTGGT GAGCTCTTTG ATCGCATAGA ACCTGATGTT
    GGAATGCCTG AGCAAGATGC ACAAAAATTT TTTCAGCAGC TAATTGCTGG TGTGGAATAC
    CTGCACAGCA TTGGAATAAC GCACAGAGAT ATTAAGCCTG AGAACTTACT CTTAGATGAC
    CGAGATCACC TGAAAATCTC TGACTTTGGT TTGGCAACAG TGTTCCGACA CAATGCGAAA
    GAAAGACTTT TAAACAAGAT GTGTGGAACC CTACCCTATG TTGCACCAGA ACTGATTAAG
    TCCAGAGCCT TTAATGCAGA GCCAGTAGAT GTGTGGTCAT GTGGCATTGT ACTGACTGCC
    ATGTTAGCCG GAGAGTTACC ATGGGATCAG CCAACTGAAG CATGCCAGGA GTATTGTGAT
    TGGAAGGAAA AAAATCATTA TCTCACTCCA TGGAAAAAAA TAGGTGCTAC CCCTCTTGCA
    TTGCTGTGTA AAATGTTAAC AGAAAATCCA CAAAGCAGAA TCACCATTCC AGACATAAAG
    AAGGACCGTT GGTTCACAGA ATTAATCAAG AAAGGGATTA AGAGAAGCCG TGCTTTCTCT
    GGAGGTACTT CAGATTCTTC TGGGCTTTGT AAGCAGATCC GATCAGATAT GGAGATTTCA
    ATCATCTCTC ACAGTGAAGA GAAGAATGCA TATTCTAGTA CACAGCCAGA GCCACGGACT
    GCACTGGCAA CATGGGACAG CAATGGTTCT CATATAGACA ATTTGGTGCA GGGAAAAGGC
    ATCAGCTTCT CTCAGCCTGC CTGTCCTGAA AACATGCTTC TGAGCAGCCA GTTAATTGGT
    ACTCCAGGCT CCTCTCAGAA TGTTTGGCAA CGTTTGGTCA AAAGAATGAC CAGGTTCTTT
    ACAAAAGTGG ATGCTGAAAG CTCGTACAGC AATTTGATGG ACACTTGTGA AAAAATGGGA
    TATGTTTTAA AGAAGTCTTG TGCTAACCAG GTCACCCTAT CAACTACTGA CAGAAGAAAT
    AATAAATTAA TATTCAAGGT GAACCTTGTG GAAATGGAAG ACAGGATTCT GTTAGATTTT
    AGACTTTCTA AGGGTGATGG GCTGGAGTTT AAAAGGCATT TCCTGAAGAT TAAGAAGAAG
    ATGGATGCTA TTGTTGCAGT CCAAAAAGTT CTGCCTGCTA CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa13013
    Clone ID Related Accession (Same CDS sequence) XM_002937515.4 , XM_012966987.2 , XM_002937515.5 , XM_012966987.3 , XM_004916034.3 , XM_004916034.4
    Accession Version XM_004916034.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1425bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product serine/threonine-protein kinase Chk1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004916034.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCAGTTC CATTTGTTGA AGACTGGGAT CTTGTCCAGA CTCTTGGGGA AGGGGCATAT 
    GGAGAAGTGC AGCTGGCAGT AAACCGGAAA ACAGAAGAAG CAGTAGCAGT GAAGATTGTG
    GACATGACAC GTGCAGCTGA TTGCCCAGAA AACATCAAAA AGGAGATTTG TATTAATAGG
    ATGCTTAATC ACACAAATAT TGTAAGATTT TATGGACATC GACGGGAAGG AAACATTCAG
    TACCTCTTTC TGGAGTATTG TCGAGGTGGT GAGCTCTTTG ATCGCATAGA ACCTGATGTT
    GGAATGCCTG AGCAAGATGC ACAAAAATTT TTTCAGCAGC TAATTGCTGG TGTGGAATAC
    CTGCACAGCA TTGGAATAAC GCACAGAGAT ATTAAGCCTG AGAACTTACT CTTAGATGAC
    CGAGATCACC TGAAAATCTC TGACTTTGGT TTGGCAACAG TGTTCCGACA CAATGCGAAA
    GAAAGACTTT TAAACAAGAT GTGTGGAACC CTACCCTATG TTGCACCAGA ACTGATTAAG
    TCCAGAGCCT TTAATGCAGA GCCAGTAGAT GTGTGGTCAT GTGGCATTGT ACTGACTGCC
    ATGTTAGCCG GAGAGTTACC ATGGGATCAG CCAACTGAAG CATGCCAGGA GTATTGTGAT
    TGGAAGGAAA AAAATCATTA TCTCACTCCA TGGAAAAAAA TAGGTGCTAC CCCTCTTGCA
    TTGCTGTGTA AAATGTTAAC AGAAAATCCA CAAAGCAGAA TCACCATTCC AGACATAAAG
    AAGGACCGTT GGTTCACAGA ATTAATCAAG AAAGGGATTA AGAGAAGCCG TGCTTTCTCT
    GGAGGTACTT CAGATTCTTC TGGGCTTTGT AAGCAGATCC GATCAGATAT GGAGATTTCA
    ATCATCTCTC ACAGTGAAGA GAAGAATGCA TATTCTAGTA CACAGCCAGA GCCACGGACT
    GCACTGGCAA CATGGGACAG CAATGGTTCT CATATAGACA ATTTGGTGCA GGGAAAAGGC
    ATCAGCTTCT CTCAGCCTGC CTGTCCTGAA AACATGCTTC TGAGCAGCCA GTTAATTGGT
    ACTCCAGGCT CCTCTCAGAA TGTTTGGCAA CGTTTGGTCA AAAGAATGAC CAGGTTCTTT
    ACAAAAGTGG ATGCTGAAAG CTCGTACAGC AATTTGATGG ACACTTGTGA AAAAATGGGA
    TATGTTTTAA AGAAGTCTTG TGCTAACCAG GTCACCCTAT CAACTACTGA CAGAAGAAAT
    AATAAATTAA TATTCAAGGT GAACCTTGTG GAAATGGAAG ACAGGATTCT GTTAGATTTT
    AGACTTTCTA AGGGTGATGG GCTGGAGTTT AAAAGGCATT TCCTGAAGAT TAAGAAGAAG
    ATGGATGCTA TTGTTGCAGT CCAAAAAGTT CTGCCTGCTA CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004916091.1
    CDS276..1700
    Translation

    Target ORF information:

    RefSeq Version XM_004916034.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis checkpoint kinase 1 (chek1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004916034.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCAGTTC CATTTGTTGA AGACTGGGAT CTTGTCCAGA CTCTTGGGGA AGGGGCATAT 
    GGAGAAGTGC AGCTGGCAGT AAACCGGAAA ACAGAAGAAG CAGTAGCAGT GAAGATTGTG
    GACATGACAC GTGCAGCTGA TTGCCCAGAA AACATCAAAA AGGAGATTTG TATTAATAGG
    ATGCTTAATC ACACAAATAT TGTAAGATTT TATGGACATC GACGGGAAGG AAACATTCAG
    TACCTCTTTC TGGAGTATTG TCGAGGTGGT GAGCTCTTTG ATCGCATAGA ACCTGATGTT
    GGAATGCCTG AGCAAGATGC ACAAAAATTT TTTCAGCAGC TAATTGCTGG TGTGGAATAC
    CTGCACAGCA TTGGAATAAC GCACAGAGAT ATTAAGCCTG AGAACTTACT CTTAGATGAC
    CGAGATCACC TGAAAATCTC TGACTTTGGT TTGGCAACAG TGTTCCGACA CAATGCGAAA
    GAAAGACTTT TAAACAAGAT GTGTGGAACC CTACCCTATG TTGCACCAGA ACTGATTAAG
    TCCAGAGCCT TTAATGCAGA GCCAGTAGAT GTGTGGTCAT GTGGCATTGT ACTGACTGCC
    ATGTTAGCCG GAGAGTTACC ATGGGATCAG CCAACTGAAG CATGCCAGGA GTATTGTGAT
    TGGAAGGAAA AAAATCATTA TCTCACTCCA TGGAAAAAAA TAGGTGCTAC CCCTCTTGCA
    TTGCTGTGTA AAATGTTAAC AGAAAATCCA CAAAGCAGAA TCACCATTCC AGACATAAAG
    AAGGACCGTT GGTTCACAGA ATTAATCAAG AAAGGGATTA AGAGAAGCCG TGCTTTCTCT
    GGAGGTACTT CAGATTCTTC TGGGCTTTGT AAGCAGATCC GATCAGATAT GGAGATTTCA
    ATCATCTCTC ACAGTGAAGA GAAGAATGCA TATTCTAGTA CACAGCCAGA GCCACGGACT
    GCACTGGCAA CATGGGACAG CAATGGTTCT CATATAGACA ATTTGGTGCA GGGAAAAGGC
    ATCAGCTTCT CTCAGCCTGC CTGTCCTGAA AACATGCTTC TGAGCAGCCA GTTAATTGGT
    ACTCCAGGCT CCTCTCAGAA TGTTTGGCAA CGTTTGGTCA AAAGAATGAC CAGGTTCTTT
    ACAAAAGTGG ATGCTGAAAG CTCGTACAGC AATTTGATGG ACACTTGTGA AAAAATGGGA
    TATGTTTTAA AGAAGTCTTG TGCTAACCAG GTCACCCTAT CAACTACTGA CAGAAGAAAT
    AATAAATTAA TATTCAAGGT GAACCTTGTG GAAATGGAAG ACAGGATTCT GTTAGATTTT
    AGACTTTCTA AGGGTGATGG GCTGGAGTTT AAAAGGCATT TCCTGAAGAT TAAGAAGAAG
    ATGGATGCTA TTGTTGCAGT CCAAAAAGTT CTGCCTGCTA CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa13013
    Clone ID Related Accession (Same CDS sequence) XM_002937515.4 , XM_012966987.2 , XM_002937515.5 , XM_012966987.3 , XM_004916034.3 , XM_004916034.4
    Accession Version XM_002937515.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1425bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product serine/threonine-protein kinase Chk1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002937515.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCAGTTC CATTTGTTGA AGACTGGGAT CTTGTCCAGA CTCTTGGGGA AGGGGCATAT 
    GGAGAAGTGC AGCTGGCAGT AAACCGGAAA ACAGAAGAAG CAGTAGCAGT GAAGATTGTG
    GACATGACAC GTGCAGCTGA TTGCCCAGAA AACATCAAAA AGGAGATTTG TATTAATAGG
    ATGCTTAATC ACACAAATAT TGTAAGATTT TATGGACATC GACGGGAAGG AAACATTCAG
    TACCTCTTTC TGGAGTATTG TCGAGGTGGT GAGCTCTTTG ATCGCATAGA ACCTGATGTT
    GGAATGCCTG AGCAAGATGC ACAAAAATTT TTTCAGCAGC TAATTGCTGG TGTGGAATAC
    CTGCACAGCA TTGGAATAAC GCACAGAGAT ATTAAGCCTG AGAACTTACT CTTAGATGAC
    CGAGATCACC TGAAAATCTC TGACTTTGGT TTGGCAACAG TGTTCCGACA CAATGCGAAA
    GAAAGACTTT TAAACAAGAT GTGTGGAACC CTACCCTATG TTGCACCAGA ACTGATTAAG
    TCCAGAGCCT TTAATGCAGA GCCAGTAGAT GTGTGGTCAT GTGGCATTGT ACTGACTGCC
    ATGTTAGCCG GAGAGTTACC ATGGGATCAG CCAACTGAAG CATGCCAGGA GTATTGTGAT
    TGGAAGGAAA AAAATCATTA TCTCACTCCA TGGAAAAAAA TAGGTGCTAC CCCTCTTGCA
    TTGCTGTGTA AAATGTTAAC AGAAAATCCA CAAAGCAGAA TCACCATTCC AGACATAAAG
    AAGGACCGTT GGTTCACAGA ATTAATCAAG AAAGGGATTA AGAGAAGCCG TGCTTTCTCT
    GGAGGTACTT CAGATTCTTC TGGGCTTTGT AAGCAGATCC GATCAGATAT GGAGATTTCA
    ATCATCTCTC ACAGTGAAGA GAAGAATGCA TATTCTAGTA CACAGCCAGA GCCACGGACT
    GCACTGGCAA CATGGGACAG CAATGGTTCT CATATAGACA ATTTGGTGCA GGGAAAAGGC
    ATCAGCTTCT CTCAGCCTGC CTGTCCTGAA AACATGCTTC TGAGCAGCCA GTTAATTGGT
    ACTCCAGGCT CCTCTCAGAA TGTTTGGCAA CGTTTGGTCA AAAGAATGAC CAGGTTCTTT
    ACAAAAGTGG ATGCTGAAAG CTCGTACAGC AATTTGATGG ACACTTGTGA AAAAATGGGA
    TATGTTTTAA AGAAGTCTTG TGCTAACCAG GTCACCCTAT CAACTACTGA CAGAAGAAAT
    AATAAATTAA TATTCAAGGT GAACCTTGTG GAAATGGAAG ACAGGATTCT GTTAGATTTT
    AGACTTTCTA AGGGTGATGG GCTGGAGTTT AAAAGGCATT TCCTGAAGAT TAAGAAGAAG
    ATGGATGCTA TTGTTGCAGT CCAAAAAGTT CTGCCTGCTA CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002937561.2
    CDS260..1684
    Translation

    Target ORF information:

    RefSeq Version XM_002937515.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis checkpoint kinase 1 (chek1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002937515.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCAGTTC CATTTGTTGA AGACTGGGAT CTTGTCCAGA CTCTTGGGGA AGGGGCATAT 
    GGAGAAGTGC AGCTGGCAGT AAACCGGAAA ACAGAAGAAG CAGTAGCAGT GAAGATTGTG
    GACATGACAC GTGCAGCTGA TTGCCCAGAA AACATCAAAA AGGAGATTTG TATTAATAGG
    ATGCTTAATC ACACAAATAT TGTAAGATTT TATGGACATC GACGGGAAGG AAACATTCAG
    TACCTCTTTC TGGAGTATTG TCGAGGTGGT GAGCTCTTTG ATCGCATAGA ACCTGATGTT
    GGAATGCCTG AGCAAGATGC ACAAAAATTT TTTCAGCAGC TAATTGCTGG TGTGGAATAC
    CTGCACAGCA TTGGAATAAC GCACAGAGAT ATTAAGCCTG AGAACTTACT CTTAGATGAC
    CGAGATCACC TGAAAATCTC TGACTTTGGT TTGGCAACAG TGTTCCGACA CAATGCGAAA
    GAAAGACTTT TAAACAAGAT GTGTGGAACC CTACCCTATG TTGCACCAGA ACTGATTAAG
    TCCAGAGCCT TTAATGCAGA GCCAGTAGAT GTGTGGTCAT GTGGCATTGT ACTGACTGCC
    ATGTTAGCCG GAGAGTTACC ATGGGATCAG CCAACTGAAG CATGCCAGGA GTATTGTGAT
    TGGAAGGAAA AAAATCATTA TCTCACTCCA TGGAAAAAAA TAGGTGCTAC CCCTCTTGCA
    TTGCTGTGTA AAATGTTAAC AGAAAATCCA CAAAGCAGAA TCACCATTCC AGACATAAAG
    AAGGACCGTT GGTTCACAGA ATTAATCAAG AAAGGGATTA AGAGAAGCCG TGCTTTCTCT
    GGAGGTACTT CAGATTCTTC TGGGCTTTGT AAGCAGATCC GATCAGATAT GGAGATTTCA
    ATCATCTCTC ACAGTGAAGA GAAGAATGCA TATTCTAGTA CACAGCCAGA GCCACGGACT
    GCACTGGCAA CATGGGACAG CAATGGTTCT CATATAGACA ATTTGGTGCA GGGAAAAGGC
    ATCAGCTTCT CTCAGCCTGC CTGTCCTGAA AACATGCTTC TGAGCAGCCA GTTAATTGGT
    ACTCCAGGCT CCTCTCAGAA TGTTTGGCAA CGTTTGGTCA AAAGAATGAC CAGGTTCTTT
    ACAAAAGTGG ATGCTGAAAG CTCGTACAGC AATTTGATGG ACACTTGTGA AAAAATGGGA
    TATGTTTTAA AGAAGTCTTG TGCTAACCAG GTCACCCTAT CAACTACTGA CAGAAGAAAT
    AATAAATTAA TATTCAAGGT GAACCTTGTG GAAATGGAAG ACAGGATTCT GTTAGATTTT
    AGACTTTCTA AGGGTGATGG GCTGGAGTTT AAAAGGCATT TCCTGAAGAT TAAGAAGAAG
    ATGGATGCTA TTGTTGCAGT CCAAAAAGTT CTGCCTGCTA CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa13013
    Clone ID Related Accession (Same CDS sequence) XM_002937515.4 , XM_012966987.2 , XM_002937515.5 , XM_012966987.3 , XM_004916034.3 , XM_004916034.4
    Accession Version XM_012966987.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1425bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product serine/threonine-protein kinase Chk1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966987.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCAGTTC CATTTGTTGA AGACTGGGAT CTTGTCCAGA CTCTTGGGGA AGGGGCATAT 
    GGAGAAGTGC AGCTGGCAGT AAACCGGAAA ACAGAAGAAG CAGTAGCAGT GAAGATTGTG
    GACATGACAC GTGCAGCTGA TTGCCCAGAA AACATCAAAA AGGAGATTTG TATTAATAGG
    ATGCTTAATC ACACAAATAT TGTAAGATTT TATGGACATC GACGGGAAGG AAACATTCAG
    TACCTCTTTC TGGAGTATTG TCGAGGTGGT GAGCTCTTTG ATCGCATAGA ACCTGATGTT
    GGAATGCCTG AGCAAGATGC ACAAAAATTT TTTCAGCAGC TAATTGCTGG TGTGGAATAC
    CTGCACAGCA TTGGAATAAC GCACAGAGAT ATTAAGCCTG AGAACTTACT CTTAGATGAC
    CGAGATCACC TGAAAATCTC TGACTTTGGT TTGGCAACAG TGTTCCGACA CAATGCGAAA
    GAAAGACTTT TAAACAAGAT GTGTGGAACC CTACCCTATG TTGCACCAGA ACTGATTAAG
    TCCAGAGCCT TTAATGCAGA GCCAGTAGAT GTGTGGTCAT GTGGCATTGT ACTGACTGCC
    ATGTTAGCCG GAGAGTTACC ATGGGATCAG CCAACTGAAG CATGCCAGGA GTATTGTGAT
    TGGAAGGAAA AAAATCATTA TCTCACTCCA TGGAAAAAAA TAGGTGCTAC CCCTCTTGCA
    TTGCTGTGTA AAATGTTAAC AGAAAATCCA CAAAGCAGAA TCACCATTCC AGACATAAAG
    AAGGACCGTT GGTTCACAGA ATTAATCAAG AAAGGGATTA AGAGAAGCCG TGCTTTCTCT
    GGAGGTACTT CAGATTCTTC TGGGCTTTGT AAGCAGATCC GATCAGATAT GGAGATTTCA
    ATCATCTCTC ACAGTGAAGA GAAGAATGCA TATTCTAGTA CACAGCCAGA GCCACGGACT
    GCACTGGCAA CATGGGACAG CAATGGTTCT CATATAGACA ATTTGGTGCA GGGAAAAGGC
    ATCAGCTTCT CTCAGCCTGC CTGTCCTGAA AACATGCTTC TGAGCAGCCA GTTAATTGGT
    ACTCCAGGCT CCTCTCAGAA TGTTTGGCAA CGTTTGGTCA AAAGAATGAC CAGGTTCTTT
    ACAAAAGTGG ATGCTGAAAG CTCGTACAGC AATTTGATGG ACACTTGTGA AAAAATGGGA
    TATGTTTTAA AGAAGTCTTG TGCTAACCAG GTCACCCTAT CAACTACTGA CAGAAGAAAT
    AATAAATTAA TATTCAAGGT GAACCTTGTG GAAATGGAAG ACAGGATTCT GTTAGATTTT
    AGACTTTCTA AGGGTGATGG GCTGGAGTTT AAAAGGCATT TCCTGAAGAT TAAGAAGAAG
    ATGGATGCTA TTGTTGCAGT CCAAAAAGTT CTGCCTGCTA CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012822441.1
    CDS211..1635
    Translation

    Target ORF information:

    RefSeq Version XM_012966987.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis checkpoint kinase 1 (chek1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966987.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCAGTTC CATTTGTTGA AGACTGGGAT CTTGTCCAGA CTCTTGGGGA AGGGGCATAT 
    GGAGAAGTGC AGCTGGCAGT AAACCGGAAA ACAGAAGAAG CAGTAGCAGT GAAGATTGTG
    GACATGACAC GTGCAGCTGA TTGCCCAGAA AACATCAAAA AGGAGATTTG TATTAATAGG
    ATGCTTAATC ACACAAATAT TGTAAGATTT TATGGACATC GACGGGAAGG AAACATTCAG
    TACCTCTTTC TGGAGTATTG TCGAGGTGGT GAGCTCTTTG ATCGCATAGA ACCTGATGTT
    GGAATGCCTG AGCAAGATGC ACAAAAATTT TTTCAGCAGC TAATTGCTGG TGTGGAATAC
    CTGCACAGCA TTGGAATAAC GCACAGAGAT ATTAAGCCTG AGAACTTACT CTTAGATGAC
    CGAGATCACC TGAAAATCTC TGACTTTGGT TTGGCAACAG TGTTCCGACA CAATGCGAAA
    GAAAGACTTT TAAACAAGAT GTGTGGAACC CTACCCTATG TTGCACCAGA ACTGATTAAG
    TCCAGAGCCT TTAATGCAGA GCCAGTAGAT GTGTGGTCAT GTGGCATTGT ACTGACTGCC
    ATGTTAGCCG GAGAGTTACC ATGGGATCAG CCAACTGAAG CATGCCAGGA GTATTGTGAT
    TGGAAGGAAA AAAATCATTA TCTCACTCCA TGGAAAAAAA TAGGTGCTAC CCCTCTTGCA
    TTGCTGTGTA AAATGTTAAC AGAAAATCCA CAAAGCAGAA TCACCATTCC AGACATAAAG
    AAGGACCGTT GGTTCACAGA ATTAATCAAG AAAGGGATTA AGAGAAGCCG TGCTTTCTCT
    GGAGGTACTT CAGATTCTTC TGGGCTTTGT AAGCAGATCC GATCAGATAT GGAGATTTCA
    ATCATCTCTC ACAGTGAAGA GAAGAATGCA TATTCTAGTA CACAGCCAGA GCCACGGACT
    GCACTGGCAA CATGGGACAG CAATGGTTCT CATATAGACA ATTTGGTGCA GGGAAAAGGC
    ATCAGCTTCT CTCAGCCTGC CTGTCCTGAA AACATGCTTC TGAGCAGCCA GTTAATTGGT
    ACTCCAGGCT CCTCTCAGAA TGTTTGGCAA CGTTTGGTCA AAAGAATGAC CAGGTTCTTT
    ACAAAAGTGG ATGCTGAAAG CTCGTACAGC AATTTGATGG ACACTTGTGA AAAAATGGGA
    TATGTTTTAA AGAAGTCTTG TGCTAACCAG GTCACCCTAT CAACTACTGA CAGAAGAAAT
    AATAAATTAA TATTCAAGGT GAACCTTGTG GAAATGGAAG ACAGGATTCT GTTAGATTTT
    AGACTTTCTA AGGGTGATGG GCTGGAGTTT AAAAGGCATT TCCTGAAGAT TAAGAAGAAG
    ATGGATGCTA TTGTTGCAGT CCAAAAAGTT CTGCCTGCTA CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa13013
    Clone ID Related Accession (Same CDS sequence) XM_002937515.4 , XM_012966987.2 , XM_002937515.5 , XM_012966987.3 , XM_004916034.3 , XM_004916034.4
    Accession Version XM_004916034.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1425bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product serine/threonine-protein kinase Chk1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004916034.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCAGTTC CATTTGTTGA AGACTGGGAT CTTGTCCAGA CTCTTGGGGA AGGGGCATAT 
    GGAGAAGTGC AGCTGGCAGT AAACCGGAAA ACAGAAGAAG CAGTAGCAGT GAAGATTGTG
    GACATGACAC GTGCAGCTGA TTGCCCAGAA AACATCAAAA AGGAGATTTG TATTAATAGG
    ATGCTTAATC ACACAAATAT TGTAAGATTT TATGGACATC GACGGGAAGG AAACATTCAG
    TACCTCTTTC TGGAGTATTG TCGAGGTGGT GAGCTCTTTG ATCGCATAGA ACCTGATGTT
    GGAATGCCTG AGCAAGATGC ACAAAAATTT TTTCAGCAGC TAATTGCTGG TGTGGAATAC
    CTGCACAGCA TTGGAATAAC GCACAGAGAT ATTAAGCCTG AGAACTTACT CTTAGATGAC
    CGAGATCACC TGAAAATCTC TGACTTTGGT TTGGCAACAG TGTTCCGACA CAATGCGAAA
    GAAAGACTTT TAAACAAGAT GTGTGGAACC CTACCCTATG TTGCACCAGA ACTGATTAAG
    TCCAGAGCCT TTAATGCAGA GCCAGTAGAT GTGTGGTCAT GTGGCATTGT ACTGACTGCC
    ATGTTAGCCG GAGAGTTACC ATGGGATCAG CCAACTGAAG CATGCCAGGA GTATTGTGAT
    TGGAAGGAAA AAAATCATTA TCTCACTCCA TGGAAAAAAA TAGGTGCTAC CCCTCTTGCA
    TTGCTGTGTA AAATGTTAAC AGAAAATCCA CAAAGCAGAA TCACCATTCC AGACATAAAG
    AAGGACCGTT GGTTCACAGA ATTAATCAAG AAAGGGATTA AGAGAAGCCG TGCTTTCTCT
    GGAGGTACTT CAGATTCTTC TGGGCTTTGT AAGCAGATCC GATCAGATAT GGAGATTTCA
    ATCATCTCTC ACAGTGAAGA GAAGAATGCA TATTCTAGTA CACAGCCAGA GCCACGGACT
    GCACTGGCAA CATGGGACAG CAATGGTTCT CATATAGACA ATTTGGTGCA GGGAAAAGGC
    ATCAGCTTCT CTCAGCCTGC CTGTCCTGAA AACATGCTTC TGAGCAGCCA GTTAATTGGT
    ACTCCAGGCT CCTCTCAGAA TGTTTGGCAA CGTTTGGTCA AAAGAATGAC CAGGTTCTTT
    ACAAAAGTGG ATGCTGAAAG CTCGTACAGC AATTTGATGG ACACTTGTGA AAAAATGGGA
    TATGTTTTAA AGAAGTCTTG TGCTAACCAG GTCACCCTAT CAACTACTGA CAGAAGAAAT
    AATAAATTAA TATTCAAGGT GAACCTTGTG GAAATGGAAG ACAGGATTCT GTTAGATTTT
    AGACTTTCTA AGGGTGATGG GCTGGAGTTT AAAAGGCATT TCCTGAAGAT TAAGAAGAAG
    ATGGATGCTA TTGTTGCAGT CCAAAAAGTT CTGCCTGCTA CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004916091.1
    CDS247..1671
    Translation

    Target ORF information:

    RefSeq Version XM_004916034.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis checkpoint kinase 1 (chek1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004916034.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCAGTTC CATTTGTTGA AGACTGGGAT CTTGTCCAGA CTCTTGGGGA AGGGGCATAT 
    GGAGAAGTGC AGCTGGCAGT AAACCGGAAA ACAGAAGAAG CAGTAGCAGT GAAGATTGTG
    GACATGACAC GTGCAGCTGA TTGCCCAGAA AACATCAAAA AGGAGATTTG TATTAATAGG
    ATGCTTAATC ACACAAATAT TGTAAGATTT TATGGACATC GACGGGAAGG AAACATTCAG
    TACCTCTTTC TGGAGTATTG TCGAGGTGGT GAGCTCTTTG ATCGCATAGA ACCTGATGTT
    GGAATGCCTG AGCAAGATGC ACAAAAATTT TTTCAGCAGC TAATTGCTGG TGTGGAATAC
    CTGCACAGCA TTGGAATAAC GCACAGAGAT ATTAAGCCTG AGAACTTACT CTTAGATGAC
    CGAGATCACC TGAAAATCTC TGACTTTGGT TTGGCAACAG TGTTCCGACA CAATGCGAAA
    GAAAGACTTT TAAACAAGAT GTGTGGAACC CTACCCTATG TTGCACCAGA ACTGATTAAG
    TCCAGAGCCT TTAATGCAGA GCCAGTAGAT GTGTGGTCAT GTGGCATTGT ACTGACTGCC
    ATGTTAGCCG GAGAGTTACC ATGGGATCAG CCAACTGAAG CATGCCAGGA GTATTGTGAT
    TGGAAGGAAA AAAATCATTA TCTCACTCCA TGGAAAAAAA TAGGTGCTAC CCCTCTTGCA
    TTGCTGTGTA AAATGTTAAC AGAAAATCCA CAAAGCAGAA TCACCATTCC AGACATAAAG
    AAGGACCGTT GGTTCACAGA ATTAATCAAG AAAGGGATTA AGAGAAGCCG TGCTTTCTCT
    GGAGGTACTT CAGATTCTTC TGGGCTTTGT AAGCAGATCC GATCAGATAT GGAGATTTCA
    ATCATCTCTC ACAGTGAAGA GAAGAATGCA TATTCTAGTA CACAGCCAGA GCCACGGACT
    GCACTGGCAA CATGGGACAG CAATGGTTCT CATATAGACA ATTTGGTGCA GGGAAAAGGC
    ATCAGCTTCT CTCAGCCTGC CTGTCCTGAA AACATGCTTC TGAGCAGCCA GTTAATTGGT
    ACTCCAGGCT CCTCTCAGAA TGTTTGGCAA CGTTTGGTCA AAAGAATGAC CAGGTTCTTT
    ACAAAAGTGG ATGCTGAAAG CTCGTACAGC AATTTGATGG ACACTTGTGA AAAAATGGGA
    TATGTTTTAA AGAAGTCTTG TGCTAACCAG GTCACCCTAT CAACTACTGA CAGAAGAAAT
    AATAAATTAA TATTCAAGGT GAACCTTGTG GAAATGGAAG ACAGGATTCT GTTAGATTTT
    AGACTTTCTA AGGGTGATGG GCTGGAGTTT AAAAGGCATT TCCTGAAGAT TAAGAAGAAG
    ATGGATGCTA TTGTTGCAGT CCAAAAAGTT CTGCCTGCTA CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.