syngap1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following syngap1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the syngap1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa46369 XM_018096726.1
Latest version!
Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OXa46370 XM_018096727.1
Latest version!
Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OXa46371 XM_018096728.1
Latest version!
Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OXa46372 XM_018096729.1
Latest version!
Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OXa46373 XM_018096730.1
Latest version!
Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OXa46374 XM_018096731.1
Latest version!
Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OXa46375 XM_018096732.1
Latest version!
Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OXa46376 XM_018096733.1
Latest version!
Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OXa46377 XM_018096734.1
Latest version!
Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OXa46378 XM_018096735.1
Latest version!
Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OXa46379 XM_018096736.1
Latest version!
Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OXa46380 XM_018096737.1
Latest version!
Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OXa65724 XM_031892369.1
Latest version!
Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa65725 XM_031892370.1
Latest version!
Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa65726 XM_031892371.1
Latest version!
Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa65727 XM_031892372.1
Latest version!
Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa65728 XM_031892373.1
Latest version!
Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa65729 XM_031892374.1
Latest version!
Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa65730 XM_031892375.1
Latest version!
Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa65730 XM_031892377.1
Latest version!
Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa65731 XM_031892378.1
Latest version!
Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa65732 XM_031892379.1
Latest version!
Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa65733 XM_031892380.1
Latest version!
Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa65734 XM_031892381.1
Latest version!
Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa65735 XM_031892382.1
Latest version!
Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa65736 XM_031892383.1
Latest version!
Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa65737 XM_031892384.1
Latest version!
Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa65738 XM_031892385.1
Latest version!
Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa46369
    Clone ID Related Accession (Same CDS sequence) XM_018096726.1
    Accession Version XM_018096726.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3702bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ras/Rap GTPase-activating protein SynGAP isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGATGTTCT ATGCTCCCTT CAGAGATGCT CGAGGACCTC CCATGCATCG TATGCCCTAT 
    GTCACCTCTC CATACGACCG TCCAGCCTGG AACCCAAGAT ACTGTGTCCT CTCTGGAAAT
    CAGCTTCTCA TGCTTGATGA GGAGGAGGTT CACCCGCTGT TGATACGTGA AAGGAGAACT
    GATCCAACCA GAACAAAGTT CCTAAGGCGC ACAGTCAGTG TCCCCGTGGA GGGGAAGCAG
    CCTGACGAAC ACGATTATCA CCTTGCACGT TCCCGGAGGA AAAGCGTCCC AGGGGGAAAA
    CAGCCCAGTA TGGACGCCCC TCCCACCGCC CCCTTTCGAC CGTCGCAAGG ATTCCTGAGC
    CGTCGCTTGA AGAGCTCTAT AAAGAGGACA AAGAGTCAGC CCAAATTAGA TAGGACCAGC
    AGTTTCCGGC AAATATTGCC AAGGTTTCGA AGTGCTGATC ATGATAGGGC ACGGCTCATG
    CAGGCCTTTA AAGAGTCACA CTCTCACGAG TCCTTGCTTA GTCCTAGCAG TGCTGCAGAG
    GCTTTGGATT TGAACTTGGA TGAGGATTCT GTGGTGAAGC CTGTACACAG CAGCATCTTG
    GGACAGGAGT TTTGCTTCGA GGTGACCACA TCATCAGGTA CCAAATGTTT TGCCTGCCGT
    TCTGCTGCTG AAAGAGACAA ATGGATTGAG AATCTTCAAA GAGCTGTAAA GCCCAATAAG
    GATAACAGCA GGCGGGTAGA TAATGTCTTA AAGCTGTGGA TTATTGAAGC CCGTGATCTT
    CCCCCCAAGA AGCGCTATTA TTGTGAGCTC TGTTTGGATG ACATTTTGTA TGCCCGTACC
    ACCAGCAAGC CTCGGAATGA CACTGTCTTT TGGGGGGAAC ACTTTGAATT TAATAACCTT
    CCAGCTGTTC GGAACTTGCG CTTGCACCTC TATAAGGACA CAGACAAGAA AAGGCGCAAA
    GAAAAGAGCA ACTATGTGGG CATGGTGAAC GTGCCTATTG CCAGCATTAG TGGGCGCCAC
    TTTGTGGAGC AGTGGTACCC GGTTTCTCAG CCCACATCCT ACAAGGGGAA GAGCAGCTGT
    CCCTCCATTC GCCTAAAATC CCGATACCAA ACCATGAGCA TCCTCCCCAT GGAGCTGTAC
    AAGGAGTTTG CAGAGTATGT CACCAACAAC TACCGGATGC TGTGCGCTGT ACTGGAGCCT
    GTGCTTAGCG TGAAGAGCAA AGAGGAGGTT GCCTGTGCTT TAGTGCATAT ACTGCAAAGC
    ACAGGCAAGG CCAAGGACTT CCTTTCAGAC ATGGCTATGA CCGAGGTTGA TCGCTTCCTT
    GACAGAGAGC ACCTTATTCT CCGTGAGAAC ACACTTGCAA CTAAAGCCAT AGAGGAGTAT
    CTGAAACTGA TAGGACAGAG ATACCTCAAG GAAGCCATTG GTGAGTTTAT TCGGGCACTG
    TATGAGTCTG AGGAGGACTG TGAGGTTGAT CCCATGCGTT GTCCACCCTC TGCATTGCCC
    GATCACCAGG CCAATCTCAG GATGTGCTGT GAACTCGCCC TTTGCAAAGT CTTTAACTCT
    CATTGTGTGT TTCCTCGGGA GTTGAAGGAA GTCTTTGCAT CTTGGAGGAT GCGCTGTGCA
    GAGCGCGGCC GGGAAGATAT AGCAGATCGA CTCATCAGTG CCTCTCTCTT CCTCCGATTC
    CTGTGTCCGG CCATTATGTC CCCCAGTCTC TTCAGCCTAA TGCAGGAATA CCCTGATGAG
    CAAACATCTA GGACCCTCAC GCTCATCGCA AAAGTCATCC AGAACCTGGC CAACTTCAAC
    TACAACAGGT TCAGCAGTAA AGAAGAATAT ATGTCCTTTA TGAATGAGTT CCTAGAGACG
    GAGTTGGCGA ATATGCAGAA TTTCCTGTAT GAGATCTCAA ACCTGGATTC GCTCACCAAC
    AGCATTAACT TTGAGGGCTA CATTGACCTT GGGCGGGAGC TGAGTACACT TCATGCCCTG
    CTTTGGGAAG TGATCCCACA ACTTAGTAAG GATGCACTTA TAAAGTTGGG GCCTCTTCCA
    CGCCTCCTTA ATGACATCAG CCTAGCTCTC AGGAACCCCC ACATCCAACG ACAACCAAGC
    CACCAAGAAC GACTGCCACC CCCCAAACCT GTCATTCTGA GGGGACCTTC AGCTGAGCTG
    CAAAGCTACT TGGTGCGAGA CCTCAACAGC TCTATTGACA TGACTCGCCT ACCCTCACCT
    ACTAAGGATA AACCATCAAA AGATATGTTT TTTGTTTCAC GTCGCCCGCT AACTCGCTCC
    TCGCCAGCCT ATTGCACCAG CAGCTCAGAC ATCACAGAAC CAGACCAGAA GATGCTGAGC
    GTGAACAAGA GTGTGTCCAT GATGGACTTG CAGGACAGCC GAATGAATAG CGTAACCAAC
    TTGCAGGCCG TAGGTGATCT GCTGAACTCC AGCCAGGCCT CCATATCAGG GGGCCTACGT
    CCGGGGGGCC GGCTCTCCCA GGGCAGTGGA TCCAGCATTG CAGCGGGTCT GAGGCTCAGT
    CAGATGGGGG TCACCACTGA TGGGCTCTCA GCTCAACAGC TACGCATTCC CTTGTCCTTC
    CAGAACCCGC TCTTCCACAT GGCTCCCGAT GGGCCTGGTG GGCCCCCATC TCATCCTGCT
    CCTCATCGAC CAGACCCCGG AGCTGATGCC TTTGCCCCTT TCCATGGGTA TAGCAAGAGT
    GAGGACTTGA GCTCCGGAAA GCATATGCTT CACAGTCACA GCTACAGCGA CGAATTCAGC
    CGGCAGGGTA AAAGGCAGTT ATCCCTCCAG GACAATCTCC AGCACATGCT TTCACCCCCT
    CAAATAACCA TTGGCCCTGG AGTGCCACGA AGGCAAGACT CACAGCCTCC CCCTTTGCAG
    AGGGGAAAAT CCCAACAACT GACGGTGCAG GCAGCGCATA AACCTCGGCC AAGCAGTGGC
    AACCTCCTAC AGAGCCCAGA ACCAATGTAT GGGGCAGCAA GAATTAGGCA GCAGTCAGTT
    GAAAAGGATG CAGGTGGCTT GAAACCATCA ATAACCAAGC AGCACTCCCA TGCACAGGCC
    ACTCTGAACC CTGTCACTCC AGCCTCAGAA CGCACAGTTG CCTGGGTATC CAACATGCCC
    CACCTTTCAG CAGATATTGA GAGCGCTCAC ATAGAACGGG AGGAGTATAA GTTGAAGGAA
    TACTCTAAAT CAATGGATGA GAGCCGTCTT GAGAGGGTGA AAGAGTATGA GGAAGAGATT
    TACTCCTTAA AAGAGAGGCT GTTGATGTCA AACCGGAAGC TGGAGGAATA TGAGAGGCGT
    CTTCTTACAC AGGAAGAACA AACTAATAAG ATGATGCAGC AATATCAGTC ACGCCTGGAC
    CAGAGTGAAA AGCGTTTGCG ACAGCAGCAG GTTGAGAAGG ACACGCAGAT AAAGAGCATC
    TTAAACAGGT TAGAAATGGT TGATGCTGGT GGAGGAGGAG ATCAGGAAGG ATCACTCACA
    AGAACCAATC GATATGAAGA GGAGACTTAT CGAGGCTCAG GTGGAAATTA CAATGTCATT
    GTGCGTTTGT GTGTGACATC TTCATCACAC CCTGTCCCTG TCTTTCATCT CAAGAGGGAG
    AGAAGAAAAA AAAAAACAAG GATACAAAGA AATCTAGGGC TTAATGAAGA AATAGGGGGG
    AGAAGAAGTT TCCCTCCGTG GGTACAGCAG ACCCGCGTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017952215.1
    CDS945..4646
    Translation

    Target ORF information:

    RefSeq Version XM_018096726.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018096726.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGATGTTCT ATGCTCCCTT CAGAGATGCT CGAGGACCTC CCATGCATCG TATGCCCTAT 
    GTCACCTCTC CATACGACCG TCCAGCCTGG AACCCAAGAT ACTGTGTCCT CTCTGGAAAT
    CAGCTTCTCA TGCTTGATGA GGAGGAGGTT CACCCGCTGT TGATACGTGA AAGGAGAACT
    GATCCAACCA GAACAAAGTT CCTAAGGCGC ACAGTCAGTG TCCCCGTGGA GGGGAAGCAG
    CCTGACGAAC ACGATTATCA CCTTGCACGT TCCCGGAGGA AAAGCGTCCC AGGGGGAAAA
    CAGCCCAGTA TGGACGCCCC TCCCACCGCC CCCTTTCGAC CGTCGCAAGG ATTCCTGAGC
    CGTCGCTTGA AGAGCTCTAT AAAGAGGACA AAGAGTCAGC CCAAATTAGA TAGGACCAGC
    AGTTTCCGGC AAATATTGCC AAGGTTTCGA AGTGCTGATC ATGATAGGGC ACGGCTCATG
    CAGGCCTTTA AAGAGTCACA CTCTCACGAG TCCTTGCTTA GTCCTAGCAG TGCTGCAGAG
    GCTTTGGATT TGAACTTGGA TGAGGATTCT GTGGTGAAGC CTGTACACAG CAGCATCTTG
    GGACAGGAGT TTTGCTTCGA GGTGACCACA TCATCAGGTA CCAAATGTTT TGCCTGCCGT
    TCTGCTGCTG AAAGAGACAA ATGGATTGAG AATCTTCAAA GAGCTGTAAA GCCCAATAAG
    GATAACAGCA GGCGGGTAGA TAATGTCTTA AAGCTGTGGA TTATTGAAGC CCGTGATCTT
    CCCCCCAAGA AGCGCTATTA TTGTGAGCTC TGTTTGGATG ACATTTTGTA TGCCCGTACC
    ACCAGCAAGC CTCGGAATGA CACTGTCTTT TGGGGGGAAC ACTTTGAATT TAATAACCTT
    CCAGCTGTTC GGAACTTGCG CTTGCACCTC TATAAGGACA CAGACAAGAA AAGGCGCAAA
    GAAAAGAGCA ACTATGTGGG CATGGTGAAC GTGCCTATTG CCAGCATTAG TGGGCGCCAC
    TTTGTGGAGC AGTGGTACCC GGTTTCTCAG CCCACATCCT ACAAGGGGAA GAGCAGCTGT
    CCCTCCATTC GCCTAAAATC CCGATACCAA ACCATGAGCA TCCTCCCCAT GGAGCTGTAC
    AAGGAGTTTG CAGAGTATGT CACCAACAAC TACCGGATGC TGTGCGCTGT ACTGGAGCCT
    GTGCTTAGCG TGAAGAGCAA AGAGGAGGTT GCCTGTGCTT TAGTGCATAT ACTGCAAAGC
    ACAGGCAAGG CCAAGGACTT CCTTTCAGAC ATGGCTATGA CCGAGGTTGA TCGCTTCCTT
    GACAGAGAGC ACCTTATTCT CCGTGAGAAC ACACTTGCAA CTAAAGCCAT AGAGGAGTAT
    CTGAAACTGA TAGGACAGAG ATACCTCAAG GAAGCCATTG GTGAGTTTAT TCGGGCACTG
    TATGAGTCTG AGGAGGACTG TGAGGTTGAT CCCATGCGTT GTCCACCCTC TGCATTGCCC
    GATCACCAGG CCAATCTCAG GATGTGCTGT GAACTCGCCC TTTGCAAAGT CTTTAACTCT
    CATTGTGTGT TTCCTCGGGA GTTGAAGGAA GTCTTTGCAT CTTGGAGGAT GCGCTGTGCA
    GAGCGCGGCC GGGAAGATAT AGCAGATCGA CTCATCAGTG CCTCTCTCTT CCTCCGATTC
    CTGTGTCCGG CCATTATGTC CCCCAGTCTC TTCAGCCTAA TGCAGGAATA CCCTGATGAG
    CAAACATCTA GGACCCTCAC GCTCATCGCA AAAGTCATCC AGAACCTGGC CAACTTCAAC
    TACAACAGGT TCAGCAGTAA AGAAGAATAT ATGTCCTTTA TGAATGAGTT CCTAGAGACG
    GAGTTGGCGA ATATGCAGAA TTTCCTGTAT GAGATCTCAA ACCTGGATTC GCTCACCAAC
    AGCATTAACT TTGAGGGCTA CATTGACCTT GGGCGGGAGC TGAGTACACT TCATGCCCTG
    CTTTGGGAAG TGATCCCACA ACTTAGTAAG GATGCACTTA TAAAGTTGGG GCCTCTTCCA
    CGCCTCCTTA ATGACATCAG CCTAGCTCTC AGGAACCCCC ACATCCAACG ACAACCAAGC
    CACCAAGAAC GACTGCCACC CCCCAAACCT GTCATTCTGA GGGGACCTTC AGCTGAGCTG
    CAAAGCTACT TGGTGCGAGA CCTCAACAGC TCTATTGACA TGACTCGCCT ACCCTCACCT
    ACTAAGGATA AACCATCAAA AGATATGTTT TTTGTTTCAC GTCGCCCGCT AACTCGCTCC
    TCGCCAGCCT ATTGCACCAG CAGCTCAGAC ATCACAGAAC CAGACCAGAA GATGCTGAGC
    GTGAACAAGA GTGTGTCCAT GATGGACTTG CAGGACAGCC GAATGAATAG CGTAACCAAC
    TTGCAGGCCG TAGGTGATCT GCTGAACTCC AGCCAGGCCT CCATATCAGG GGGCCTACGT
    CCGGGGGGCC GGCTCTCCCA GGGCAGTGGA TCCAGCATTG CAGCGGGTCT GAGGCTCAGT
    CAGATGGGGG TCACCACTGA TGGGCTCTCA GCTCAACAGC TACGCATTCC CTTGTCCTTC
    CAGAACCCGC TCTTCCACAT GGCTCCCGAT GGGCCTGGTG GGCCCCCATC TCATCCTGCT
    CCTCATCGAC CAGACCCCGG AGCTGATGCC TTTGCCCCTT TCCATGGGTA TAGCAAGAGT
    GAGGACTTGA GCTCCGGAAA GCATATGCTT CACAGTCACA GCTACAGCGA CGAATTCAGC
    CGGCAGGGTA AAAGGCAGTT ATCCCTCCAG GACAATCTCC AGCACATGCT TTCACCCCCT
    CAAATAACCA TTGGCCCTGG AGTGCCACGA AGGCAAGACT CACAGCCTCC CCCTTTGCAG
    AGGGGAAAAT CCCAACAACT GACGGTGCAG GCAGCGCATA AACCTCGGCC AAGCAGTGGC
    AACCTCCTAC AGAGCCCAGA ACCAATGTAT GGGGCAGCAA GAATTAGGCA GCAGTCAGTT
    GAAAAGGATG CAGGTGGCTT GAAACCATCA ATAACCAAGC AGCACTCCCA TGCACAGGCC
    ACTCTGAACC CTGTCACTCC AGCCTCAGAA CGCACAGTTG CCTGGGTATC CAACATGCCC
    CACCTTTCAG CAGATATTGA GAGCGCTCAC ATAGAACGGG AGGAGTATAA GTTGAAGGAA
    TACTCTAAAT CAATGGATGA GAGCCGTCTT GAGAGGGTGA AAGAGTATGA GGAAGAGATT
    TACTCCTTAA AAGAGAGGCT GTTGATGTCA AACCGGAAGC TGGAGGAATA TGAGAGGCGT
    CTTCTTACAC AGGAAGAACA AACTAATAAG ATGATGCAGC AATATCAGTC ACGCCTGGAC
    CAGAGTGAAA AGCGTTTGCG ACAGCAGCAG GTTGAGAAGG ACACGCAGAT AAAGAGCATC
    TTAAACAGGT TAGAAATGGT TGATGCTGGT GGAGGAGGAG ATCAGGAAGG ATCACTCACA
    AGAACCAATC GATATGAAGA GGAGACTTAT CGAGGCTCAG GTGGAAATTA CAATGTCATT
    GTGCGTTTGT GTGTGACATC TTCATCACAC CCTGTCCCTG TCTTTCATCT CAAGAGGGAG
    AGAAGAAAAA AAAAAACAAG GATACAAAGA AATCTAGGGC TTAATGAAGA AATAGGGGGG
    AGAAGAAGTT TCCCTCCGTG GGTACAGCAG ACCCGCGTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa46370
    Clone ID Related Accession (Same CDS sequence) XM_018096727.1
    Accession Version XM_018096727.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3699bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ras/Rap GTPase-activating protein SynGAP isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGATGTTCT ATGCTCCCTT CAGAGATGCT CGAGGACCTC CCATGCATCG TATGCCCTAT 
    GTCACCTCTC CATACGACCG TCCAGCCTGG AACCCAAGAT ACTGTGTCCT CTCTGGAAAT
    CAGCTTCTCA TGCTTGATGA GGAGGAGGTT CACCCGCTGT TGATACGTGA AAGGAGAACT
    GATCCAACCA GAACAAAGTT CCTAAGGCGC ACAGTCAGTG TCCCCGTGGA GGGGAAGCAG
    CCTGACGAAC ACGATTATCA CCTTGCACGT TCCCGGAGGA AAAGCGTCCC AGGGGGAAAA
    CAGCCCAGTA TGGACGCCCC TCCCACCGCC CCCTTTCGAC CGTCGCAAGG ATTCCTGAGC
    CGTCGCTTGA AGAGCTCTAT AAAGAGGACA AAGAGTCAGC CCAAATTAGA TAGGACCAGC
    AGTTTCCGGC AAATATTGCC AAGGTTTCGA AGTGCTGATC ATGATAGGGC ACGGCTCATG
    CAGGCCTTTA AAGAGTCACA CTCTCACGAG TCCTTGCTTA GTCCTAGCAG TGCTGCAGAG
    GCTTTGGATT TGAACTTGGA TGAGGATTCT GTGGTGAAGC CTGTACACAG CAGCATCTTG
    GGACAGGAGT TTTGCTTCGA GGTGACCACA TCATCAGGTA CCAAATGTTT TGCCTGCCGT
    TCTGCTGCTG AAAGAGACAA ATGGATTGAG AATCTTCAAA GAGCTGTAAA GCCCAATAAG
    GATAACAGCA GGCGGGTAGA TAATGTCTTA AAGCTGTGGA TTATTGAAGC CCGTGATCTT
    CCCCCCAAGA AGCGCTATTA TTGTGAGCTC TGTTTGGATG ACATTTTGTA TGCCCGTACC
    ACCAGCAAGC CTCGGAATGA CACTGTCTTT TGGGGGGAAC ACTTTGAATT TAATAACCTT
    CCAGCTGTTC GGAACTTGCG CTTGCACCTC TATAAGGACA CAGACAAGAA AAGGCGCAAA
    GAAAAGAGCA ACTATGTGGG CATGGTGAAC GTGCCTATTG CCAGCATTAG TGGGCGCCAC
    TTTGTGGAGC AGTGGTACCC GGTTTCTCAG CCCACATCCT ACAAGGGGAA GAGCAGCTGT
    CCCTCCATTC GCCTAAAATC CCGATACCAA ACCATGAGCA TCCTCCCCAT GGAGCTGTAC
    AAGGAGTTTG CAGAGTATGT CACCAACAAC TACCGGATGC TGTGCGCTGT ACTGGAGCCT
    GTGCTTAGCG TGAAGAGCAA AGAGGAGGTT GCCTGTGCTT TAGTGCATAT ACTGCAAAGC
    ACAGGCAAGG CCAAGGACTT CCTTTCAGAC ATGGCTATGA CCGAGGTTGA TCGCTTCCTT
    GACAGAGAGC ACCTTATTCT CCGTGAGAAC ACACTTGCAA CTAAAGCCAT AGAGGAGTAT
    CTGAAACTGA TAGGACAGAG ATACCTCAAG GAAGCCATTG GTGAGTTTAT TCGGGCACTG
    TATGAGTCTG AGGAGGACTG TGAGGTTGAT CCCATGCGTT GTCCACCCTC TGCATTGCCC
    GATCACCAGG CCAATCTCAG GATGTGCTGT GAACTCGCCC TTTGCAAAGT CTTTAACTCT
    CATTGTGTGT TTCCTCGGGA GTTGAAGGAA GTCTTTGCAT CTTGGAGGAT GCGCTGTGCA
    GAGCGCGGCC GGGAAGATAT AGCAGATCGA CTCATCAGTG CCTCTCTCTT CCTCCGATTC
    CTGTGTCCGG CCATTATGTC CCCCAGTCTC TTCAGCCTAA TGCAGGAATA CCCTGATGAG
    CAAACATCTA GGACCCTCAC GCTCATCGCA AAAGTCATCC AGAACCTGGC CAACTTCAAC
    TACAAGTTCA GCAGTAAAGA AGAATATATG TCCTTTATGA ATGAGTTCCT AGAGACGGAG
    TTGGCGAATA TGCAGAATTT CCTGTATGAG ATCTCAAACC TGGATTCGCT CACCAACAGC
    ATTAACTTTG AGGGCTACAT TGACCTTGGG CGGGAGCTGA GTACACTTCA TGCCCTGCTT
    TGGGAAGTGA TCCCACAACT TAGTAAGGAT GCACTTATAA AGTTGGGGCC TCTTCCACGC
    CTCCTTAATG ACATCAGCCT AGCTCTCAGG AACCCCCACA TCCAACGACA ACCAAGCCAC
    CAAGAACGAC TGCCACCCCC CAAACCTGTC ATTCTGAGGG GACCTTCAGC TGAGCTGCAA
    AGCTACTTGG TGCGAGACCT CAACAGCTCT ATTGACATGA CTCGCCTACC CTCACCTACT
    AAGGATAAAC CATCAAAAGA TATGTTTTTT GTTTCACGTC GCCCGCTAAC TCGCTCCTCG
    CCAGCCTATT GCACCAGCAG CTCAGACATC ACAGAACCAG ACCAGAAGAT GCTGAGCGTG
    AACAAGAGTG TGTCCATGAT GGACTTGCAG GACAGCCGAA TGAATAGCGT AACCAACTTG
    CAGGCCGTAG GTGATCTGCT GAACTCCAGC CAGGCCTCCA TATCAGGGGG CCTACGTCCG
    GGGGGCCGGC TCTCCCAGGG CAGTGGATCC AGCATTGCAG CGGGTCTGAG GCTCAGTCAG
    ATGGGGGTCA CCACTGATGG GCTCTCAGCT CAACAGCTAC GCATTCCCTT GTCCTTCCAG
    AACCCGCTCT TCCACATGGC TCCCGATGGG CCTGGTGGGC CCCCATCTCA TCCTGCTCCT
    CATCGACCAG ACCCCGGAGC TGATGCCTTT GCCCCTTTCC ATGGGTATAG CAAGAGTGAG
    GACTTGAGCT CCGGAAAGCA TATGCTTCAC AGTCACAGCT ACAGCGACGA ATTCAGCCGG
    CAGGGTAAAA GGCAGTTATC CCTCCAGGAC AATCTCCAGC ACATGCTTTC ACCCCCTCAA
    ATAACCATTG GCCCTGGAGT GCCACGAAGG CAAGACTCAC AGCCTCCCCC TTTGCAGAGG
    GGAAAATCCC AACAACTGAC GGTGCAGGCA GCGCATAAAC CTCGGCCAAG CAGTGGCAAC
    CTCCTACAGA GCCCAGAACC AATGTATGGG GCAGCAAGAA TTAGGCAGCA GTCAGTTGAA
    AAGGATGCAG GTGGCTTGAA ACCATCAATA ACCAAGCAGC ACTCCCATGC ACAGGCCACT
    CTGAACCCTG TCACTCCAGC CTCAGAACGC ACAGTTGCCT GGGTATCCAA CATGCCCCAC
    CTTTCAGCAG ATATTGAGAG CGCTCACATA GAACGGGAGG AGTATAAGTT GAAGGAATAC
    TCTAAATCAA TGGATGAGAG CCGTCTTGAG AGGGTGAAAG AGTATGAGGA AGAGATTTAC
    TCCTTAAAAG AGAGGCTGTT GATGTCAAAC CGGAAGCTGG AGGAATATGA GAGGCGTCTT
    CTTACACAGG AAGAACAAAC TAATAAGATG ATGCAGCAAT ATCAGTCACG CCTGGACCAG
    AGTGAAAAGC GTTTGCGACA GCAGCAGGTT GAGAAGGACA CGCAGATAAA GAGCATCTTA
    AACAGGTTAG AAATGGTTGA TGCTGGTGGA GGAGGAGATC AGGAAGGATC ACTCACAAGA
    ACCAATCGAT ATGAAGAGGA GACTTATCGA GGCTCAGGTG GAAATTACAA TGTCATTGTG
    CGTTTGTGTG TGACATCTTC ATCACACCCT GTCCCTGTCT TTCATCTCAA GAGGGAGAGA
    AGAAAAAAAA AAACAAGGAT ACAAAGAAAT CTAGGGCTTA ATGAAGAAAT AGGGGGGAGA
    AGAAGTTTCC CTCCGTGGGT ACAGCAGACC CGCGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017952216.1
    CDS944..4642
    Translation

    Target ORF information:

    RefSeq Version XM_018096727.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018096727.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGATGTTCT ATGCTCCCTT CAGAGATGCT CGAGGACCTC CCATGCATCG TATGCCCTAT 
    GTCACCTCTC CATACGACCG TCCAGCCTGG AACCCAAGAT ACTGTGTCCT CTCTGGAAAT
    CAGCTTCTCA TGCTTGATGA GGAGGAGGTT CACCCGCTGT TGATACGTGA AAGGAGAACT
    GATCCAACCA GAACAAAGTT CCTAAGGCGC ACAGTCAGTG TCCCCGTGGA GGGGAAGCAG
    CCTGACGAAC ACGATTATCA CCTTGCACGT TCCCGGAGGA AAAGCGTCCC AGGGGGAAAA
    CAGCCCAGTA TGGACGCCCC TCCCACCGCC CCCTTTCGAC CGTCGCAAGG ATTCCTGAGC
    CGTCGCTTGA AGAGCTCTAT AAAGAGGACA AAGAGTCAGC CCAAATTAGA TAGGACCAGC
    AGTTTCCGGC AAATATTGCC AAGGTTTCGA AGTGCTGATC ATGATAGGGC ACGGCTCATG
    CAGGCCTTTA AAGAGTCACA CTCTCACGAG TCCTTGCTTA GTCCTAGCAG TGCTGCAGAG
    GCTTTGGATT TGAACTTGGA TGAGGATTCT GTGGTGAAGC CTGTACACAG CAGCATCTTG
    GGACAGGAGT TTTGCTTCGA GGTGACCACA TCATCAGGTA CCAAATGTTT TGCCTGCCGT
    TCTGCTGCTG AAAGAGACAA ATGGATTGAG AATCTTCAAA GAGCTGTAAA GCCCAATAAG
    GATAACAGCA GGCGGGTAGA TAATGTCTTA AAGCTGTGGA TTATTGAAGC CCGTGATCTT
    CCCCCCAAGA AGCGCTATTA TTGTGAGCTC TGTTTGGATG ACATTTTGTA TGCCCGTACC
    ACCAGCAAGC CTCGGAATGA CACTGTCTTT TGGGGGGAAC ACTTTGAATT TAATAACCTT
    CCAGCTGTTC GGAACTTGCG CTTGCACCTC TATAAGGACA CAGACAAGAA AAGGCGCAAA
    GAAAAGAGCA ACTATGTGGG CATGGTGAAC GTGCCTATTG CCAGCATTAG TGGGCGCCAC
    TTTGTGGAGC AGTGGTACCC GGTTTCTCAG CCCACATCCT ACAAGGGGAA GAGCAGCTGT
    CCCTCCATTC GCCTAAAATC CCGATACCAA ACCATGAGCA TCCTCCCCAT GGAGCTGTAC
    AAGGAGTTTG CAGAGTATGT CACCAACAAC TACCGGATGC TGTGCGCTGT ACTGGAGCCT
    GTGCTTAGCG TGAAGAGCAA AGAGGAGGTT GCCTGTGCTT TAGTGCATAT ACTGCAAAGC
    ACAGGCAAGG CCAAGGACTT CCTTTCAGAC ATGGCTATGA CCGAGGTTGA TCGCTTCCTT
    GACAGAGAGC ACCTTATTCT CCGTGAGAAC ACACTTGCAA CTAAAGCCAT AGAGGAGTAT
    CTGAAACTGA TAGGACAGAG ATACCTCAAG GAAGCCATTG GTGAGTTTAT TCGGGCACTG
    TATGAGTCTG AGGAGGACTG TGAGGTTGAT CCCATGCGTT GTCCACCCTC TGCATTGCCC
    GATCACCAGG CCAATCTCAG GATGTGCTGT GAACTCGCCC TTTGCAAAGT CTTTAACTCT
    CATTGTGTGT TTCCTCGGGA GTTGAAGGAA GTCTTTGCAT CTTGGAGGAT GCGCTGTGCA
    GAGCGCGGCC GGGAAGATAT AGCAGATCGA CTCATCAGTG CCTCTCTCTT CCTCCGATTC
    CTGTGTCCGG CCATTATGTC CCCCAGTCTC TTCAGCCTAA TGCAGGAATA CCCTGATGAG
    CAAACATCTA GGACCCTCAC GCTCATCGCA AAAGTCATCC AGAACCTGGC CAACTTCAAC
    TACAAGTTCA GCAGTAAAGA AGAATATATG TCCTTTATGA ATGAGTTCCT AGAGACGGAG
    TTGGCGAATA TGCAGAATTT CCTGTATGAG ATCTCAAACC TGGATTCGCT CACCAACAGC
    ATTAACTTTG AGGGCTACAT TGACCTTGGG CGGGAGCTGA GTACACTTCA TGCCCTGCTT
    TGGGAAGTGA TCCCACAACT TAGTAAGGAT GCACTTATAA AGTTGGGGCC TCTTCCACGC
    CTCCTTAATG ACATCAGCCT AGCTCTCAGG AACCCCCACA TCCAACGACA ACCAAGCCAC
    CAAGAACGAC TGCCACCCCC CAAACCTGTC ATTCTGAGGG GACCTTCAGC TGAGCTGCAA
    AGCTACTTGG TGCGAGACCT CAACAGCTCT ATTGACATGA CTCGCCTACC CTCACCTACT
    AAGGATAAAC CATCAAAAGA TATGTTTTTT GTTTCACGTC GCCCGCTAAC TCGCTCCTCG
    CCAGCCTATT GCACCAGCAG CTCAGACATC ACAGAACCAG ACCAGAAGAT GCTGAGCGTG
    AACAAGAGTG TGTCCATGAT GGACTTGCAG GACAGCCGAA TGAATAGCGT AACCAACTTG
    CAGGCCGTAG GTGATCTGCT GAACTCCAGC CAGGCCTCCA TATCAGGGGG CCTACGTCCG
    GGGGGCCGGC TCTCCCAGGG CAGTGGATCC AGCATTGCAG CGGGTCTGAG GCTCAGTCAG
    ATGGGGGTCA CCACTGATGG GCTCTCAGCT CAACAGCTAC GCATTCCCTT GTCCTTCCAG
    AACCCGCTCT TCCACATGGC TCCCGATGGG CCTGGTGGGC CCCCATCTCA TCCTGCTCCT
    CATCGACCAG ACCCCGGAGC TGATGCCTTT GCCCCTTTCC ATGGGTATAG CAAGAGTGAG
    GACTTGAGCT CCGGAAAGCA TATGCTTCAC AGTCACAGCT ACAGCGACGA ATTCAGCCGG
    CAGGGTAAAA GGCAGTTATC CCTCCAGGAC AATCTCCAGC ACATGCTTTC ACCCCCTCAA
    ATAACCATTG GCCCTGGAGT GCCACGAAGG CAAGACTCAC AGCCTCCCCC TTTGCAGAGG
    GGAAAATCCC AACAACTGAC GGTGCAGGCA GCGCATAAAC CTCGGCCAAG CAGTGGCAAC
    CTCCTACAGA GCCCAGAACC AATGTATGGG GCAGCAAGAA TTAGGCAGCA GTCAGTTGAA
    AAGGATGCAG GTGGCTTGAA ACCATCAATA ACCAAGCAGC ACTCCCATGC ACAGGCCACT
    CTGAACCCTG TCACTCCAGC CTCAGAACGC ACAGTTGCCT GGGTATCCAA CATGCCCCAC
    CTTTCAGCAG ATATTGAGAG CGCTCACATA GAACGGGAGG AGTATAAGTT GAAGGAATAC
    TCTAAATCAA TGGATGAGAG CCGTCTTGAG AGGGTGAAAG AGTATGAGGA AGAGATTTAC
    TCCTTAAAAG AGAGGCTGTT GATGTCAAAC CGGAAGCTGG AGGAATATGA GAGGCGTCTT
    CTTACACAGG AAGAACAAAC TAATAAGATG ATGCAGCAAT ATCAGTCACG CCTGGACCAG
    AGTGAAAAGC GTTTGCGACA GCAGCAGGTT GAGAAGGACA CGCAGATAAA GAGCATCTTA
    AACAGGTTAG AAATGGTTGA TGCTGGTGGA GGAGGAGATC AGGAAGGATC ACTCACAAGA
    ACCAATCGAT ATGAAGAGGA GACTTATCGA GGCTCAGGTG GAAATTACAA TGTCATTGTG
    CGTTTGTGTG TGACATCTTC ATCACACCCT GTCCCTGTCT TTCATCTCAA GAGGGAGAGA
    AGAAAAAAAA AAACAAGGAT ACAAAGAAAT CTAGGGCTTA ATGAAGAAAT AGGGGGGAGA
    AGAAGTTTCC CTCCGTGGGT ACAGCAGACC CGCGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa46371
    Clone ID Related Accession (Same CDS sequence) XM_018096728.1
    Accession Version XM_018096728.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3690bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ras/Rap GTPase-activating protein SynGAP isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCACAATA CAGATGCTCG AGGACCTCCC ATGCATCGTA TGCCCTATGT CACCTCTCCA 
    TACGACCGTC CAGCCTGGAA CCCAAGATAC TGTGTCCTCT CTGGAAATCA GCTTCTCATG
    CTTGATGAGG AGGAGGTTCA CCCGCTGTTG ATACGTGAAA GGAGAACTGA TCCAACCAGA
    ACAAAGTTCC TAAGGCGCAC AGTCAGTGTC CCCGTGGAGG GGAAGCAGCC TGACGAACAC
    GATTATCACC TTGCACGTTC CCGGAGGAAA AGCGTCCCAG GGGGAAAACA GCCCAGTATG
    GACGCCCCTC CCACCGCCCC CTTTCGACCG TCGCAAGGAT TCCTGAGCCG TCGCTTGAAG
    AGCTCTATAA AGAGGACAAA GAGTCAGCCC AAATTAGATA GGACCAGCAG TTTCCGGCAA
    ATATTGCCAA GGTTTCGAAG TGCTGATCAT GATAGGGCAC GGCTCATGCA GGCCTTTAAA
    GAGTCACACT CTCACGAGTC CTTGCTTAGT CCTAGCAGTG CTGCAGAGGC TTTGGATTTG
    AACTTGGATG AGGATTCTGT GGTGAAGCCT GTACACAGCA GCATCTTGGG ACAGGAGTTT
    TGCTTCGAGG TGACCACATC ATCAGGTACC AAATGTTTTG CCTGCCGTTC TGCTGCTGAA
    AGAGACAAAT GGATTGAGAA TCTTCAAAGA GCTGTAAAGC CCAATAAGGA TAACAGCAGG
    CGGGTAGATA ATGTCTTAAA GCTGTGGATT ATTGAAGCCC GTGATCTTCC CCCCAAGAAG
    CGCTATTATT GTGAGCTCTG TTTGGATGAC ATTTTGTATG CCCGTACCAC CAGCAAGCCT
    CGGAATGACA CTGTCTTTTG GGGGGAACAC TTTGAATTTA ATAACCTTCC AGCTGTTCGG
    AACTTGCGCT TGCACCTCTA TAAGGACACA GACAAGAAAA GGCGCAAAGA AAAGAGCAAC
    TATGTGGGCA TGGTGAACGT GCCTATTGCC AGCATTAGTG GGCGCCACTT TGTGGAGCAG
    TGGTACCCGG TTTCTCAGCC CACATCCTAC AAGGGGAAGA GCAGCTGTCC CTCCATTCGC
    CTAAAATCCC GATACCAAAC CATGAGCATC CTCCCCATGG AGCTGTACAA GGAGTTTGCA
    GAGTATGTCA CCAACAACTA CCGGATGCTG TGCGCTGTAC TGGAGCCTGT GCTTAGCGTG
    AAGAGCAAAG AGGAGGTTGC CTGTGCTTTA GTGCATATAC TGCAAAGCAC AGGCAAGGCC
    AAGGACTTCC TTTCAGACAT GGCTATGACC GAGGTTGATC GCTTCCTTGA CAGAGAGCAC
    CTTATTCTCC GTGAGAACAC ACTTGCAACT AAAGCCATAG AGGAGTATCT GAAACTGATA
    GGACAGAGAT ACCTCAAGGA AGCCATTGGT GAGTTTATTC GGGCACTGTA TGAGTCTGAG
    GAGGACTGTG AGGTTGATCC CATGCGTTGT CCACCCTCTG CATTGCCCGA TCACCAGGCC
    AATCTCAGGA TGTGCTGTGA ACTCGCCCTT TGCAAAGTCT TTAACTCTCA TTGTGTGTTT
    CCTCGGGAGT TGAAGGAAGT CTTTGCATCT TGGAGGATGC GCTGTGCAGA GCGCGGCCGG
    GAAGATATAG CAGATCGACT CATCAGTGCC TCTCTCTTCC TCCGATTCCT GTGTCCGGCC
    ATTATGTCCC CCAGTCTCTT CAGCCTAATG CAGGAATACC CTGATGAGCA AACATCTAGG
    ACCCTCACGC TCATCGCAAA AGTCATCCAG AACCTGGCCA ACTTCAACTA CAACAGGTTC
    AGCAGTAAAG AAGAATATAT GTCCTTTATG AATGAGTTCC TAGAGACGGA GTTGGCGAAT
    ATGCAGAATT TCCTGTATGA GATCTCAAAC CTGGATTCGC TCACCAACAG CATTAACTTT
    GAGGGCTACA TTGACCTTGG GCGGGAGCTG AGTACACTTC ATGCCCTGCT TTGGGAAGTG
    ATCCCACAAC TTAGTAAGGA TGCACTTATA AAGTTGGGGC CTCTTCCACG CCTCCTTAAT
    GACATCAGCC TAGCTCTCAG GAACCCCCAC ATCCAACGAC AACCAAGCCA CCAAGAACGA
    CTGCCACCCC CCAAACCTGT CATTCTGAGG GGACCTTCAG CTGAGCTGCA AAGCTACTTG
    GTGCGAGACC TCAACAGCTC TATTGACATG ACTCGCCTAC CCTCACCTAC TAAGGATAAA
    CCATCAAAAG ATATGTTTTT TGTTTCACGT CGCCCGCTAA CTCGCTCCTC GCCAGCCTAT
    TGCACCAGCA GCTCAGACAT CACAGAACCA GACCAGAAGA TGCTGAGCGT GAACAAGAGT
    GTGTCCATGA TGGACTTGCA GGACAGCCGA ATGAATAGCG TAACCAACTT GCAGGCCGTA
    GGTGATCTGC TGAACTCCAG CCAGGCCTCC ATATCAGGGG GCCTACGTCC GGGGGGCCGG
    CTCTCCCAGG GCAGTGGATC CAGCATTGCA GCGGGTCTGA GGCTCAGTCA GATGGGGGTC
    ACCACTGATG GGCTCTCAGC TCAACAGCTA CGCATTCCCT TGTCCTTCCA GAACCCGCTC
    TTCCACATGG CTCCCGATGG GCCTGGTGGG CCCCCATCTC ATCCTGCTCC TCATCGACCA
    GACCCCGGAG CTGATGCCTT TGCCCCTTTC CATGGGTATA GCAAGAGTGA GGACTTGAGC
    TCCGGAAAGC ATATGCTTCA CAGTCACAGC TACAGCGACG AATTCAGCCG GCAGGGTAAA
    AGGCAGTTAT CCCTCCAGGA CAATCTCCAG CACATGCTTT CACCCCCTCA AATAACCATT
    GGCCCTGGAG TGCCACGAAG GCAAGACTCA CAGCCTCCCC CTTTGCAGAG GGGAAAATCC
    CAACAACTGA CGGTGCAGGC AGCGCATAAA CCTCGGCCAA GCAGTGGCAA CCTCCTACAG
    AGCCCAGAAC CAATGTATGG GGCAGCAAGA ATTAGGCAGC AGTCAGTTGA AAAGGATGCA
    GGTGGCTTGA AACCATCAAT AACCAAGCAG CACTCCCATG CACAGGCCAC TCTGAACCCT
    GTCACTCCAG CCTCAGAACG CACAGTTGCC TGGGTATCCA ACATGCCCCA CCTTTCAGCA
    GATATTGAGA GCGCTCACAT AGAACGGGAG GAGTATAAGT TGAAGGAATA CTCTAAATCA
    ATGGATGAGA GCCGTCTTGA GAGGGTGAAA GAGTATGAGG AAGAGATTTA CTCCTTAAAA
    GAGAGGCTGT TGATGTCAAA CCGGAAGCTG GAGGAATATG AGAGGCGTCT TCTTACACAG
    GAAGAACAAA CTAATAAGAT GATGCAGCAA TATCAGTCAC GCCTGGACCA GAGTGAAAAG
    CGTTTGCGAC AGCAGCAGGT TGAGAAGGAC ACGCAGATAA AGAGCATCTT AAACAGGTTA
    GAAATGGTTG ATGCTGGTGG AGGAGGAGAT CAGGAAGGAT CACTCACAAG AACCAATCGA
    TATGAAGAGG AGACTTATCG AGGCTCAGGT GGAAATTACA ATGTCATTGT GCGTTTGTGT
    GTGACATCTT CATCACACCC TGTCCCTGTC TTTCATCTCA AGAGGGAGAG AAGAAAAAAA
    AAAACAAGGA TACAAAGAAA TCTAGGGCTT AATGAAGAAA TAGGGGGGAG AAGAAGTTTC
    CCTCCGTGGG TACAGCAGAC CCGCGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017952217.1
    CDS1013..4702
    Translation

    Target ORF information:

    RefSeq Version XM_018096728.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018096728.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCACAATA CAGATGCTCG AGGACCTCCC ATGCATCGTA TGCCCTATGT CACCTCTCCA 
    TACGACCGTC CAGCCTGGAA CCCAAGATAC TGTGTCCTCT CTGGAAATCA GCTTCTCATG
    CTTGATGAGG AGGAGGTTCA CCCGCTGTTG ATACGTGAAA GGAGAACTGA TCCAACCAGA
    ACAAAGTTCC TAAGGCGCAC AGTCAGTGTC CCCGTGGAGG GGAAGCAGCC TGACGAACAC
    GATTATCACC TTGCACGTTC CCGGAGGAAA AGCGTCCCAG GGGGAAAACA GCCCAGTATG
    GACGCCCCTC CCACCGCCCC CTTTCGACCG TCGCAAGGAT TCCTGAGCCG TCGCTTGAAG
    AGCTCTATAA AGAGGACAAA GAGTCAGCCC AAATTAGATA GGACCAGCAG TTTCCGGCAA
    ATATTGCCAA GGTTTCGAAG TGCTGATCAT GATAGGGCAC GGCTCATGCA GGCCTTTAAA
    GAGTCACACT CTCACGAGTC CTTGCTTAGT CCTAGCAGTG CTGCAGAGGC TTTGGATTTG
    AACTTGGATG AGGATTCTGT GGTGAAGCCT GTACACAGCA GCATCTTGGG ACAGGAGTTT
    TGCTTCGAGG TGACCACATC ATCAGGTACC AAATGTTTTG CCTGCCGTTC TGCTGCTGAA
    AGAGACAAAT GGATTGAGAA TCTTCAAAGA GCTGTAAAGC CCAATAAGGA TAACAGCAGG
    CGGGTAGATA ATGTCTTAAA GCTGTGGATT ATTGAAGCCC GTGATCTTCC CCCCAAGAAG
    CGCTATTATT GTGAGCTCTG TTTGGATGAC ATTTTGTATG CCCGTACCAC CAGCAAGCCT
    CGGAATGACA CTGTCTTTTG GGGGGAACAC TTTGAATTTA ATAACCTTCC AGCTGTTCGG
    AACTTGCGCT TGCACCTCTA TAAGGACACA GACAAGAAAA GGCGCAAAGA AAAGAGCAAC
    TATGTGGGCA TGGTGAACGT GCCTATTGCC AGCATTAGTG GGCGCCACTT TGTGGAGCAG
    TGGTACCCGG TTTCTCAGCC CACATCCTAC AAGGGGAAGA GCAGCTGTCC CTCCATTCGC
    CTAAAATCCC GATACCAAAC CATGAGCATC CTCCCCATGG AGCTGTACAA GGAGTTTGCA
    GAGTATGTCA CCAACAACTA CCGGATGCTG TGCGCTGTAC TGGAGCCTGT GCTTAGCGTG
    AAGAGCAAAG AGGAGGTTGC CTGTGCTTTA GTGCATATAC TGCAAAGCAC AGGCAAGGCC
    AAGGACTTCC TTTCAGACAT GGCTATGACC GAGGTTGATC GCTTCCTTGA CAGAGAGCAC
    CTTATTCTCC GTGAGAACAC ACTTGCAACT AAAGCCATAG AGGAGTATCT GAAACTGATA
    GGACAGAGAT ACCTCAAGGA AGCCATTGGT GAGTTTATTC GGGCACTGTA TGAGTCTGAG
    GAGGACTGTG AGGTTGATCC CATGCGTTGT CCACCCTCTG CATTGCCCGA TCACCAGGCC
    AATCTCAGGA TGTGCTGTGA ACTCGCCCTT TGCAAAGTCT TTAACTCTCA TTGTGTGTTT
    CCTCGGGAGT TGAAGGAAGT CTTTGCATCT TGGAGGATGC GCTGTGCAGA GCGCGGCCGG
    GAAGATATAG CAGATCGACT CATCAGTGCC TCTCTCTTCC TCCGATTCCT GTGTCCGGCC
    ATTATGTCCC CCAGTCTCTT CAGCCTAATG CAGGAATACC CTGATGAGCA AACATCTAGG
    ACCCTCACGC TCATCGCAAA AGTCATCCAG AACCTGGCCA ACTTCAACTA CAACAGGTTC
    AGCAGTAAAG AAGAATATAT GTCCTTTATG AATGAGTTCC TAGAGACGGA GTTGGCGAAT
    ATGCAGAATT TCCTGTATGA GATCTCAAAC CTGGATTCGC TCACCAACAG CATTAACTTT
    GAGGGCTACA TTGACCTTGG GCGGGAGCTG AGTACACTTC ATGCCCTGCT TTGGGAAGTG
    ATCCCACAAC TTAGTAAGGA TGCACTTATA AAGTTGGGGC CTCTTCCACG CCTCCTTAAT
    GACATCAGCC TAGCTCTCAG GAACCCCCAC ATCCAACGAC AACCAAGCCA CCAAGAACGA
    CTGCCACCCC CCAAACCTGT CATTCTGAGG GGACCTTCAG CTGAGCTGCA AAGCTACTTG
    GTGCGAGACC TCAACAGCTC TATTGACATG ACTCGCCTAC CCTCACCTAC TAAGGATAAA
    CCATCAAAAG ATATGTTTTT TGTTTCACGT CGCCCGCTAA CTCGCTCCTC GCCAGCCTAT
    TGCACCAGCA GCTCAGACAT CACAGAACCA GACCAGAAGA TGCTGAGCGT GAACAAGAGT
    GTGTCCATGA TGGACTTGCA GGACAGCCGA ATGAATAGCG TAACCAACTT GCAGGCCGTA
    GGTGATCTGC TGAACTCCAG CCAGGCCTCC ATATCAGGGG GCCTACGTCC GGGGGGCCGG
    CTCTCCCAGG GCAGTGGATC CAGCATTGCA GCGGGTCTGA GGCTCAGTCA GATGGGGGTC
    ACCACTGATG GGCTCTCAGC TCAACAGCTA CGCATTCCCT TGTCCTTCCA GAACCCGCTC
    TTCCACATGG CTCCCGATGG GCCTGGTGGG CCCCCATCTC ATCCTGCTCC TCATCGACCA
    GACCCCGGAG CTGATGCCTT TGCCCCTTTC CATGGGTATA GCAAGAGTGA GGACTTGAGC
    TCCGGAAAGC ATATGCTTCA CAGTCACAGC TACAGCGACG AATTCAGCCG GCAGGGTAAA
    AGGCAGTTAT CCCTCCAGGA CAATCTCCAG CACATGCTTT CACCCCCTCA AATAACCATT
    GGCCCTGGAG TGCCACGAAG GCAAGACTCA CAGCCTCCCC CTTTGCAGAG GGGAAAATCC
    CAACAACTGA CGGTGCAGGC AGCGCATAAA CCTCGGCCAA GCAGTGGCAA CCTCCTACAG
    AGCCCAGAAC CAATGTATGG GGCAGCAAGA ATTAGGCAGC AGTCAGTTGA AAAGGATGCA
    GGTGGCTTGA AACCATCAAT AACCAAGCAG CACTCCCATG CACAGGCCAC TCTGAACCCT
    GTCACTCCAG CCTCAGAACG CACAGTTGCC TGGGTATCCA ACATGCCCCA CCTTTCAGCA
    GATATTGAGA GCGCTCACAT AGAACGGGAG GAGTATAAGT TGAAGGAATA CTCTAAATCA
    ATGGATGAGA GCCGTCTTGA GAGGGTGAAA GAGTATGAGG AAGAGATTTA CTCCTTAAAA
    GAGAGGCTGT TGATGTCAAA CCGGAAGCTG GAGGAATATG AGAGGCGTCT TCTTACACAG
    GAAGAACAAA CTAATAAGAT GATGCAGCAA TATCAGTCAC GCCTGGACCA GAGTGAAAAG
    CGTTTGCGAC AGCAGCAGGT TGAGAAGGAC ACGCAGATAA AGAGCATCTT AAACAGGTTA
    GAAATGGTTG ATGCTGGTGG AGGAGGAGAT CAGGAAGGAT CACTCACAAG AACCAATCGA
    TATGAAGAGG AGACTTATCG AGGCTCAGGT GGAAATTACA ATGTCATTGT GCGTTTGTGT
    GTGACATCTT CATCACACCC TGTCCCTGTC TTTCATCTCA AGAGGGAGAG AAGAAAAAAA
    AAAACAAGGA TACAAAGAAA TCTAGGGCTT AATGAAGAAA TAGGGGGGAG AAGAAGTTTC
    CCTCCGTGGG TACAGCAGAC CCGCGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa46372
    Clone ID Related Accession (Same CDS sequence) XM_018096729.1
    Accession Version XM_018096729.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3660bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ras/Rap GTPase-activating protein SynGAP isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCATCGTA TGCCCTATGT CACCTCTCCA TACGACCGTC CAGCCTGGAA CCCAAGATAC 
    TGTGTCCTCT CTGGAAATCA GCTTCTCATG CTTGATGAGG AGGAGGTTCA CCCGCTGTTG
    ATACGTGAAA GGAGAACTGA TCCAACCAGA ACAAAGTTCC TAAGGCGCAC AGTCAGTGTC
    CCCGTGGAGG GGAAGCAGCC TGACGAACAC GATTATCACC TTGCACGTTC CCGGAGGAAA
    AGCGTCCCAG GGGGAAAACA GCCCAGTATG GACGCCCCTC CCACCGCCCC CTTTCGACCG
    TCGCAAGGAT TCCTGAGCCG TCGCTTGAAG AGCTCTATAA AGAGGACAAA GAGTCAGCCC
    AAATTAGATA GGACCAGCAG TTTCCGGCAA ATATTGCCAA GGTTTCGAAG TGCTGATCAT
    GATAGGGCAC GGCTCATGCA GGCCTTTAAA GAGTCACACT CTCACGAGTC CTTGCTTAGT
    CCTAGCAGTG CTGCAGAGGC TTTGGATTTG AACTTGGATG AGGATTCTGT GGTGAAGCCT
    GTACACAGCA GCATCTTGGG ACAGGAGTTT TGCTTCGAGG TGACCACATC ATCAGGTACC
    AAATGTTTTG CCTGCCGTTC TGCTGCTGAA AGAGACAAAT GGATTGAGAA TCTTCAAAGA
    GCTGTAAAGC CCAATAAGGA TAACAGCAGG CGGGTAGATA ATGTCTTAAA GCTGTGGATT
    ATTGAAGCCC GTGATCTTCC CCCCAAGAAG CGCTATTATT GTGAGCTCTG TTTGGATGAC
    ATTTTGTATG CCCGTACCAC CAGCAAGCCT CGGAATGACA CTGTCTTTTG GGGGGAACAC
    TTTGAATTTA ATAACCTTCC AGCTGTTCGG AACTTGCGCT TGCACCTCTA TAAGGACACA
    GACAAGAAAA GGCGCAAAGA AAAGAGCAAC TATGTGGGCA TGGTGAACGT GCCTATTGCC
    AGCATTAGTG GGCGCCACTT TGTGGAGCAG TGGTACCCGG TTTCTCAGCC CACATCCTAC
    AAGGGGAAGA GCAGCTGTCC CTCCATTCGC CTAAAATCCC GATACCAAAC CATGAGCATC
    CTCCCCATGG AGCTGTACAA GGAGTTTGCA GAGTATGTCA CCAACAACTA CCGGATGCTG
    TGCGCTGTAC TGGAGCCTGT GCTTAGCGTG AAGAGCAAAG AGGAGGTTGC CTGTGCTTTA
    GTGCATATAC TGCAAAGCAC AGGCAAGGCC AAGGACTTCC TTTCAGACAT GGCTATGACC
    GAGGTTGATC GCTTCCTTGA CAGAGAGCAC CTTATTCTCC GTGAGAACAC ACTTGCAACT
    AAAGCCATAG AGGAGTATCT GAAACTGATA GGACAGAGAT ACCTCAAGGA AGCCATTGGT
    GAGTTTATTC GGGCACTGTA TGAGTCTGAG GAGGACTGTG AGGTTGATCC CATGCGTTGT
    CCACCCTCTG CATTGCCCGA TCACCAGGCC AATCTCAGGA TGTGCTGTGA ACTCGCCCTT
    TGCAAAGTCT TTAACTCTCA TTGTGTGTTT CCTCGGGAGT TGAAGGAAGT CTTTGCATCT
    TGGAGGATGC GCTGTGCAGA GCGCGGCCGG GAAGATATAG CAGATCGACT CATCAGTGCC
    TCTCTCTTCC TCCGATTCCT GTGTCCGGCC ATTATGTCCC CCAGTCTCTT CAGCCTAATG
    CAGGAATACC CTGATGAGCA AACATCTAGG ACCCTCACGC TCATCGCAAA AGTCATCCAG
    AACCTGGCCA ACTTCAACTA CAACAGGTTC AGCAGTAAAG AAGAATATAT GTCCTTTATG
    AATGAGTTCC TAGAGACGGA GTTGGCGAAT ATGCAGAATT TCCTGTATGA GATCTCAAAC
    CTGGATTCGC TCACCAACAG CATTAACTTT GAGGGCTACA TTGACCTTGG GCGGGAGCTG
    AGTACACTTC ATGCCCTGCT TTGGGAAGTG ATCCCACAAC TTAGTAAGGA TGCACTTATA
    AAGTTGGGGC CTCTTCCACG CCTCCTTAAT GACATCAGCC TAGCTCTCAG GAACCCCCAC
    ATCCAACGAC AACCAAGCCA CCAAGAACGA CTGCCACCCC CCAAACCTGT CATTCTGAGG
    GGACCTTCAG CTGAGCTGCA AAGCTACTTG GTGCGAGACC TCAACAGCTC TATTGACATG
    ACTCGCCTAC CCTCACCTAC TAAGGATAAA CCATCAAAAG ATATGTTTTT TGTTTCACGT
    CGCCCGCTAA CTCGCTCCTC GCCAGCCTAT TGCACCAGCA GCTCAGACAT CACAGAACCA
    GACCAGAAGA TGCTGAGCGT GAACAAGAGT GTGTCCATGA TGGACTTGCA GGACAGCCGA
    ATGAATAGCG TAACCAACTT GCAGGCCGTA GGTGATCTGC TGAACTCCAG CCAGGCCTCC
    ATATCAGGGG GCCTACGTCC GGGGGGCCGG CTCTCCCAGG GCAGTGGATC CAGCATTGCA
    GCGGGTCTGA GGCTCAGTCA GATGGGGGTC ACCACTGATG GGCTCTCAGC TCAACAGCTA
    CGCATTCCCT TGTCCTTCCA GAACCCGCTC TTCCACATGG CTCCCGATGG GCCTGGTGGG
    CCCCCATCTC ATCCTGCTCC TCATCGACCA GACCCCGGAG CTGATGCCTT TGCCCCTTTC
    CATGGGTATA GCAAGAGTGA GGACTTGAGC TCCGGAAAGC ATATGCTTCA CAGTCACAGC
    TACAGCGACG AATTCAGCCG GCAGGGTAAA AGGCAGTTAT CCCTCCAGGA CAATCTCCAG
    CACATGCTTT CACCCCCTCA AATAACCATT GGCCCTGGAG TGCCACGAAG GCAAGACTCA
    CAGCCTCCCC CTTTGCAGAG GGGAAAATCC CAACAACTGA CGGTGCAGGC AGCGCATAAA
    CCTCGGCCAA GCAGTGGCAA CCTCCTACAG AGCCCAGAAC CAATGTATGG GGCAGCAAGA
    ATTAGGCAGC AGTCAGTTGA AAAGGATGCA GGTGGCTTGA AACCATCAAT AACCAAGCAG
    CACTCCCATG CACAGGCCAC TCTGAACCCT GTCACTCCAG CCTCAGAACG CACAGTTGCC
    TGGGTATCCA ACATGCCCCA CCTTTCAGCA GATATTGAGA GCGCTCACAT AGAACGGGAG
    GAGTATAAGT TGAAGGAATA CTCTAAATCA ATGGATGAGA GCCGTCTTGA GAGGGTGAAA
    GAGTATGAGG AAGAGATTTA CTCCTTAAAA GAGAGGCTGT TGATGTCAAA CCGGAAGCTG
    GAGGAATATG AGAGGCGTCT TCTTACACAG GAAGAACAAA CTAATAAGAT GATGCAGCAA
    TATCAGTCAC GCCTGGACCA GAGTGAAAAG CGTTTGCGAC AGCAGCAGGT TGAGAAGGAC
    ACGCAGATAA AGAGCATCTT AAACAGGTTA GAAATGGTTG ATGCTGGTGG AGGAGGAGAT
    CAGGAAGGAT CACTCACAAG AACCAATCGA TATGAAGAGG AGACTTATCG AGGCTCAGGT
    GGAAATTACA ATGTCATTGT GCGTTTGTGT GTGACATCTT CATCACACCC TGTCCCTGTC
    TTTCATCTCA AGAGGGAGAG AAGAAAAAAA AAAACAAGGA TACAAAGAAA TCTAGGGCTT
    AATGAAGAAA TAGGGGGGAG AAGAAGTTTC CCTCCGTGGG TACAGCAGAC CCGCGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017952218.1
    CDS55..3714
    Translation

    Target ORF information:

    RefSeq Version XM_018096729.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018096729.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCATCGTA TGCCCTATGT CACCTCTCCA TACGACCGTC CAGCCTGGAA CCCAAGATAC 
    TGTGTCCTCT CTGGAAATCA GCTTCTCATG CTTGATGAGG AGGAGGTTCA CCCGCTGTTG
    ATACGTGAAA GGAGAACTGA TCCAACCAGA ACAAAGTTCC TAAGGCGCAC AGTCAGTGTC
    CCCGTGGAGG GGAAGCAGCC TGACGAACAC GATTATCACC TTGCACGTTC CCGGAGGAAA
    AGCGTCCCAG GGGGAAAACA GCCCAGTATG GACGCCCCTC CCACCGCCCC CTTTCGACCG
    TCGCAAGGAT TCCTGAGCCG TCGCTTGAAG AGCTCTATAA AGAGGACAAA GAGTCAGCCC
    AAATTAGATA GGACCAGCAG TTTCCGGCAA ATATTGCCAA GGTTTCGAAG TGCTGATCAT
    GATAGGGCAC GGCTCATGCA GGCCTTTAAA GAGTCACACT CTCACGAGTC CTTGCTTAGT
    CCTAGCAGTG CTGCAGAGGC TTTGGATTTG AACTTGGATG AGGATTCTGT GGTGAAGCCT
    GTACACAGCA GCATCTTGGG ACAGGAGTTT TGCTTCGAGG TGACCACATC ATCAGGTACC
    AAATGTTTTG CCTGCCGTTC TGCTGCTGAA AGAGACAAAT GGATTGAGAA TCTTCAAAGA
    GCTGTAAAGC CCAATAAGGA TAACAGCAGG CGGGTAGATA ATGTCTTAAA GCTGTGGATT
    ATTGAAGCCC GTGATCTTCC CCCCAAGAAG CGCTATTATT GTGAGCTCTG TTTGGATGAC
    ATTTTGTATG CCCGTACCAC CAGCAAGCCT CGGAATGACA CTGTCTTTTG GGGGGAACAC
    TTTGAATTTA ATAACCTTCC AGCTGTTCGG AACTTGCGCT TGCACCTCTA TAAGGACACA
    GACAAGAAAA GGCGCAAAGA AAAGAGCAAC TATGTGGGCA TGGTGAACGT GCCTATTGCC
    AGCATTAGTG GGCGCCACTT TGTGGAGCAG TGGTACCCGG TTTCTCAGCC CACATCCTAC
    AAGGGGAAGA GCAGCTGTCC CTCCATTCGC CTAAAATCCC GATACCAAAC CATGAGCATC
    CTCCCCATGG AGCTGTACAA GGAGTTTGCA GAGTATGTCA CCAACAACTA CCGGATGCTG
    TGCGCTGTAC TGGAGCCTGT GCTTAGCGTG AAGAGCAAAG AGGAGGTTGC CTGTGCTTTA
    GTGCATATAC TGCAAAGCAC AGGCAAGGCC AAGGACTTCC TTTCAGACAT GGCTATGACC
    GAGGTTGATC GCTTCCTTGA CAGAGAGCAC CTTATTCTCC GTGAGAACAC ACTTGCAACT
    AAAGCCATAG AGGAGTATCT GAAACTGATA GGACAGAGAT ACCTCAAGGA AGCCATTGGT
    GAGTTTATTC GGGCACTGTA TGAGTCTGAG GAGGACTGTG AGGTTGATCC CATGCGTTGT
    CCACCCTCTG CATTGCCCGA TCACCAGGCC AATCTCAGGA TGTGCTGTGA ACTCGCCCTT
    TGCAAAGTCT TTAACTCTCA TTGTGTGTTT CCTCGGGAGT TGAAGGAAGT CTTTGCATCT
    TGGAGGATGC GCTGTGCAGA GCGCGGCCGG GAAGATATAG CAGATCGACT CATCAGTGCC
    TCTCTCTTCC TCCGATTCCT GTGTCCGGCC ATTATGTCCC CCAGTCTCTT CAGCCTAATG
    CAGGAATACC CTGATGAGCA AACATCTAGG ACCCTCACGC TCATCGCAAA AGTCATCCAG
    AACCTGGCCA ACTTCAACTA CAACAGGTTC AGCAGTAAAG AAGAATATAT GTCCTTTATG
    AATGAGTTCC TAGAGACGGA GTTGGCGAAT ATGCAGAATT TCCTGTATGA GATCTCAAAC
    CTGGATTCGC TCACCAACAG CATTAACTTT GAGGGCTACA TTGACCTTGG GCGGGAGCTG
    AGTACACTTC ATGCCCTGCT TTGGGAAGTG ATCCCACAAC TTAGTAAGGA TGCACTTATA
    AAGTTGGGGC CTCTTCCACG CCTCCTTAAT GACATCAGCC TAGCTCTCAG GAACCCCCAC
    ATCCAACGAC AACCAAGCCA CCAAGAACGA CTGCCACCCC CCAAACCTGT CATTCTGAGG
    GGACCTTCAG CTGAGCTGCA AAGCTACTTG GTGCGAGACC TCAACAGCTC TATTGACATG
    ACTCGCCTAC CCTCACCTAC TAAGGATAAA CCATCAAAAG ATATGTTTTT TGTTTCACGT
    CGCCCGCTAA CTCGCTCCTC GCCAGCCTAT TGCACCAGCA GCTCAGACAT CACAGAACCA
    GACCAGAAGA TGCTGAGCGT GAACAAGAGT GTGTCCATGA TGGACTTGCA GGACAGCCGA
    ATGAATAGCG TAACCAACTT GCAGGCCGTA GGTGATCTGC TGAACTCCAG CCAGGCCTCC
    ATATCAGGGG GCCTACGTCC GGGGGGCCGG CTCTCCCAGG GCAGTGGATC CAGCATTGCA
    GCGGGTCTGA GGCTCAGTCA GATGGGGGTC ACCACTGATG GGCTCTCAGC TCAACAGCTA
    CGCATTCCCT TGTCCTTCCA GAACCCGCTC TTCCACATGG CTCCCGATGG GCCTGGTGGG
    CCCCCATCTC ATCCTGCTCC TCATCGACCA GACCCCGGAG CTGATGCCTT TGCCCCTTTC
    CATGGGTATA GCAAGAGTGA GGACTTGAGC TCCGGAAAGC ATATGCTTCA CAGTCACAGC
    TACAGCGACG AATTCAGCCG GCAGGGTAAA AGGCAGTTAT CCCTCCAGGA CAATCTCCAG
    CACATGCTTT CACCCCCTCA AATAACCATT GGCCCTGGAG TGCCACGAAG GCAAGACTCA
    CAGCCTCCCC CTTTGCAGAG GGGAAAATCC CAACAACTGA CGGTGCAGGC AGCGCATAAA
    CCTCGGCCAA GCAGTGGCAA CCTCCTACAG AGCCCAGAAC CAATGTATGG GGCAGCAAGA
    ATTAGGCAGC AGTCAGTTGA AAAGGATGCA GGTGGCTTGA AACCATCAAT AACCAAGCAG
    CACTCCCATG CACAGGCCAC TCTGAACCCT GTCACTCCAG CCTCAGAACG CACAGTTGCC
    TGGGTATCCA ACATGCCCCA CCTTTCAGCA GATATTGAGA GCGCTCACAT AGAACGGGAG
    GAGTATAAGT TGAAGGAATA CTCTAAATCA ATGGATGAGA GCCGTCTTGA GAGGGTGAAA
    GAGTATGAGG AAGAGATTTA CTCCTTAAAA GAGAGGCTGT TGATGTCAAA CCGGAAGCTG
    GAGGAATATG AGAGGCGTCT TCTTACACAG GAAGAACAAA CTAATAAGAT GATGCAGCAA
    TATCAGTCAC GCCTGGACCA GAGTGAAAAG CGTTTGCGAC AGCAGCAGGT TGAGAAGGAC
    ACGCAGATAA AGAGCATCTT AAACAGGTTA GAAATGGTTG ATGCTGGTGG AGGAGGAGAT
    CAGGAAGGAT CACTCACAAG AACCAATCGA TATGAAGAGG AGACTTATCG AGGCTCAGGT
    GGAAATTACA ATGTCATTGT GCGTTTGTGT GTGACATCTT CATCACACCC TGTCCCTGTC
    TTTCATCTCA AGAGGGAGAG AAGAAAAAAA AAAACAAGGA TACAAAGAAA TCTAGGGCTT
    AATGAAGAAA TAGGGGGGAG AAGAAGTTTC CCTCCGTGGG TACAGCAGAC CCGCGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa46373
    Clone ID Related Accession (Same CDS sequence) XM_018096730.1
    Accession Version XM_018096730.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3651bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ras/Rap GTPase-activating protein SynGAP isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGATGTTCT ATGCTCCCTT CAGAGATGCT CGAGGACCTC CCATGCATCG TATGCCCTAT 
    GTCACCTCTC CATACGACCG TCCAGCCTGG AACCCAAGAT ACTGTGTCCT CTCTGGAAAT
    CAGCTTCTCA TGCTTGATGA GGAGGAGGTT CACCCGCTGT TGATACGTGA AAGGAGAACT
    GATCCAACCA GAACAAAGTT CCTAAGGCGC ACAGTCAGTG TCCCCGTGGA GGGGAAGCAG
    CCTGACGAAC ACGATTATCA CCTTGCACGT TCCCGGAGGA AAAGCGTCCC AGGGGGAAAA
    CAGCCCAGTA TGGACGCCCC TCCCACCGCC CCCTTTCGAC CGTCGCAAGG ATTCCTGAGC
    CGTCGCTTGA AGAGCTCTAT AAAGAGGACA AAGAGTCAGC CCAAATTAGA TAGGACCAGC
    AGTTTCCGGC AAATATTGCC AAGGTTTCGA AGTGCTGATC ATGATAGGGC ACGGCTCATG
    CAGGCCTTTA AAGAGTCACA CTCTCACGAG TCCTTGCTTA GTCCTAGCAG TGCTGCAGAG
    GCTTTGGATT TGAACTTGGA TGAGGATTCT GTGGTGAAGC CTGTACACAG CAGCATCTTG
    GGACAGGAGT TTTGCTTCGA GGTGACCACA TCATCAGGTA CCAAATGTTT TGCCTGCCGT
    TCTGCTGCTG AAAGAGACAA ATGGATTGAG AATCTTCAAA GAGCTGTAAA GCCCAATAAG
    GATAACAGCA GGCGGGTAGA TAATGTCTTA AAGCTGTGGA TTATTGAAGC CCGTGATCTT
    CCCCCCAAGA AGCGCTATTA TTGTGAGCTC TGTTTGGATG ACATTTTGTA TGCCCGTACC
    ACCAGCAAGC CTCGGAATGA CACTGTCTTT TGGGGGGAAC ACTTTGAATT TAATAACCTT
    CCAGCTGTTC GGAACTTGCG CTTGCACCTC TATAAGGACA CAGACAAGAA AAGGCGCAAA
    GAAAAGAGCA ACTATGTGGG CATGGTGAAC GTGCCTATTG CCAGCATTAG TGGGCGCCAC
    TTTGTGGAGC AGTGGTACCC GGTTTCTCAG CCCACATCCT ACAAGGGGAA GAGCAGCTGT
    CCCTCCATTC GCCTAAAATC CCGATACCAA ACCATGAGCA TCCTCCCCAT GGAGCTGTAC
    AAGGAGTTTG CAGAGTATGT CACCAACAAC TACCGGATGC TGTGCGCTGT ACTGGAGCCT
    GTGCTTAGCG TGAAGAGCAA AGAGGAGGTT GCCTGTGCTT TAGTGCATAT ACTGCAAAGC
    ACAGGCAAGG CCAAGGACTT CCTTTCAGAC ATGGCTATGA CCGAGGTTGA TCGCTTCCTT
    GACAGAGAGC ACCTTATTCT CCGTGAGAAC ACACTTGCAA CTAAAGCCAT AGAGGAGTAT
    CTGAAACTGA TAGGACAGAG ATACCTCAAG GAAGCCATTG GTGAGTTTAT TCGGGCACTG
    TATGAGTCTG AGGAGGACTG TGAGGTTGAT CCCATGCGTT GTCCACCCTC TGCATTGCCC
    GATCACCAGG CCAATCTCAG GATGTGCTGT GAACTCGCCC TTTGCAAAGT CTTTAACTCT
    CATTGTGTGT TTCCTCGGGA GTTGAAGGAA GTCTTTGCAT CTTGGAGGAT GCGCTGTGCA
    GAGCGCGGCC GGGAAGATAT AGCAGATCGA CTCATCAGTG CCTCTCTCTT CCTCCGATTC
    CTGTGTCCGG CCATTATGTC CCCCAGTCTC TTCAGCCTAA TGCAGGAATA CCCTGATGAG
    CAAACATCTA GGACCCTCAC GCTCATCGCA AAAGTCATCC AGAACCTGGC CAACTTCAAC
    TACAACAGGT TCAGCAGTAA AGAAGAATAT ATGTCCTTTA TGAATGAGTT CCTAGAGACG
    GAGTTGGCGA ATATGCAGAA TTTCCTGTAT GAGATCTCAA ACCTGGATTC GCTCACCAAC
    AGCATTAACT TTGAGGGCTA CATTGACCTT GGGCGGGAGC TGAGTACACT TCATGCCCTG
    CTTTGGGAAG TGATCCCACA ACTTAGTAAG GATGCACTTA TAAAGTTGGG GCCTCTTCCA
    CGCCTCCTTA ATGACATCAG CCTAGCTCTC AGGAACCCCC ACATCCAACG ACAACCAAGC
    CACCAAGAAC GACTGCCACC CCCCAAACCT GTCATTCTGA GGGGACCTTC AGCTGAGCTG
    CAAAGCTACT TGGTGCGAGA CCTCAACAGC TCTATTGACA TGACTCGCCT ACCCTCACCT
    ACTAAGGATA AACCATCAAA AGATATGTTT TTTGTTTCAC GTCGCCCGCT AACTCGCTCC
    TCGCCAGCCT ATTGCACCAG CAGCTCAGAC ATCACAGAAC CAGACCAGAA GATGCTGAGC
    GTGAACAAGA GTGTGTCCAT GATGGACTTG CAGGACAGCC GAATGAATAG CGTAACCAAC
    TTGCAGGCCG TAGGTGATCT GCTGAACTCC AGCCAGGCCT CCATATCAGG GGGCCTACGT
    CCGGGGGGCC GGCTCTCCCA GGGCAGTGGA TCCAGCATTG CAGCGGGTCT GAGGCTCAGT
    CAGATGGGGG TCACCACTGA TGGGCTCTCA GCTCAACAGC TACGCATTCC CTTGTCCTTC
    CAGAACCCGC TCTTCCACAT GGCTCCCGAT GGGCCTGGTG GGCCCCCATC TCATCCTGCT
    CCTCATCGAC CAGACCCCGG AGCTGATGCC TTTGCCCCTT TCCATGGGTA TAGCAAGAGT
    GAGGACTTGA GCTCCGGAAA GCATATGCTT CACAGTCACA GCTACAGCGA CGAATTCAGC
    CGGCAGGGTA AAAGGCAGTT ATCCCTCCAG GACAATCTCC AGCACATGCT TTCACCCCCT
    CAAATAACCA TTGGCCCTGG AGTGCCACGA AGGCAAGACT CACAGCCTCC CCCTTTGCAG
    AGGGGAAAAT CCCAACAACT GACGGTGCAG GCAGCGCATA AACCTCGGCC AAGCAGTGGC
    AACCTCCTAC AGAGCCCAGA ACCAATGTAT GGGGCAGCAA GAATTAGGCA GCAGTCAGTT
    GAAAAGGATG CAGGTGGCTT GAAACCATCA ATAACCAAGC AGCACTCCCA TGCACAGGCC
    ACTCTGAACC CTGTCACTCC AGCCTCAGAA CGCACAGTTG CCTGGGTATC CAACATGCCC
    CACCTTTCAG CAGATATTGA GAGCGCTCAC ATAGAACGGG AGGAGTATAA GTTGAAGGAA
    TACTCTAAAT CAATGGATGA GAGCCGTCTT GAGAGGGTGA AAGAGTATGA GGAAGAGATT
    TACTCCTTAA AAGAGAGGCT GTTGATGTCA AACCGGAAGC TGGAGGAATA TGAGAGGCGT
    CTTCTTACAC AGGAAGAACA AACTAATAAG ATGATGCAGC AATATCAGTC ACGCCTGGAC
    CAGAGTGAAA AGCGTTTGCG ACAGCAGCAG GTTGAGAAGG ACACGCAGAT AAAGAGCATC
    TTAAACAGGT TGATGCTGGT GGAGGAGGAG ATCAGGAAGG ATCACTCACA AGAACCAATC
    GATATGAAGA GGAGACTTAT CGAGGCTCAG GAGAAGAAGT TTCCCTCCGT GGGTACAGCA
    GACCCGCGTC TGAGGCTGGC GACTCCATGG AATGGGGCCC TCCCGCCTCT GCCTCCTCGA
    CTCCAAATCA CAGAAAATGG AGACTTTCTA AACTCAAGTG ACCATCACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017952219.1
    CDS941..4591
    Translation

    Target ORF information:

    RefSeq Version XM_018096730.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018096730.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGATGTTCT ATGCTCCCTT CAGAGATGCT CGAGGACCTC CCATGCATCG TATGCCCTAT 
    GTCACCTCTC CATACGACCG TCCAGCCTGG AACCCAAGAT ACTGTGTCCT CTCTGGAAAT
    CAGCTTCTCA TGCTTGATGA GGAGGAGGTT CACCCGCTGT TGATACGTGA AAGGAGAACT
    GATCCAACCA GAACAAAGTT CCTAAGGCGC ACAGTCAGTG TCCCCGTGGA GGGGAAGCAG
    CCTGACGAAC ACGATTATCA CCTTGCACGT TCCCGGAGGA AAAGCGTCCC AGGGGGAAAA
    CAGCCCAGTA TGGACGCCCC TCCCACCGCC CCCTTTCGAC CGTCGCAAGG ATTCCTGAGC
    CGTCGCTTGA AGAGCTCTAT AAAGAGGACA AAGAGTCAGC CCAAATTAGA TAGGACCAGC
    AGTTTCCGGC AAATATTGCC AAGGTTTCGA AGTGCTGATC ATGATAGGGC ACGGCTCATG
    CAGGCCTTTA AAGAGTCACA CTCTCACGAG TCCTTGCTTA GTCCTAGCAG TGCTGCAGAG
    GCTTTGGATT TGAACTTGGA TGAGGATTCT GTGGTGAAGC CTGTACACAG CAGCATCTTG
    GGACAGGAGT TTTGCTTCGA GGTGACCACA TCATCAGGTA CCAAATGTTT TGCCTGCCGT
    TCTGCTGCTG AAAGAGACAA ATGGATTGAG AATCTTCAAA GAGCTGTAAA GCCCAATAAG
    GATAACAGCA GGCGGGTAGA TAATGTCTTA AAGCTGTGGA TTATTGAAGC CCGTGATCTT
    CCCCCCAAGA AGCGCTATTA TTGTGAGCTC TGTTTGGATG ACATTTTGTA TGCCCGTACC
    ACCAGCAAGC CTCGGAATGA CACTGTCTTT TGGGGGGAAC ACTTTGAATT TAATAACCTT
    CCAGCTGTTC GGAACTTGCG CTTGCACCTC TATAAGGACA CAGACAAGAA AAGGCGCAAA
    GAAAAGAGCA ACTATGTGGG CATGGTGAAC GTGCCTATTG CCAGCATTAG TGGGCGCCAC
    TTTGTGGAGC AGTGGTACCC GGTTTCTCAG CCCACATCCT ACAAGGGGAA GAGCAGCTGT
    CCCTCCATTC GCCTAAAATC CCGATACCAA ACCATGAGCA TCCTCCCCAT GGAGCTGTAC
    AAGGAGTTTG CAGAGTATGT CACCAACAAC TACCGGATGC TGTGCGCTGT ACTGGAGCCT
    GTGCTTAGCG TGAAGAGCAA AGAGGAGGTT GCCTGTGCTT TAGTGCATAT ACTGCAAAGC
    ACAGGCAAGG CCAAGGACTT CCTTTCAGAC ATGGCTATGA CCGAGGTTGA TCGCTTCCTT
    GACAGAGAGC ACCTTATTCT CCGTGAGAAC ACACTTGCAA CTAAAGCCAT AGAGGAGTAT
    CTGAAACTGA TAGGACAGAG ATACCTCAAG GAAGCCATTG GTGAGTTTAT TCGGGCACTG
    TATGAGTCTG AGGAGGACTG TGAGGTTGAT CCCATGCGTT GTCCACCCTC TGCATTGCCC
    GATCACCAGG CCAATCTCAG GATGTGCTGT GAACTCGCCC TTTGCAAAGT CTTTAACTCT
    CATTGTGTGT TTCCTCGGGA GTTGAAGGAA GTCTTTGCAT CTTGGAGGAT GCGCTGTGCA
    GAGCGCGGCC GGGAAGATAT AGCAGATCGA CTCATCAGTG CCTCTCTCTT CCTCCGATTC
    CTGTGTCCGG CCATTATGTC CCCCAGTCTC TTCAGCCTAA TGCAGGAATA CCCTGATGAG
    CAAACATCTA GGACCCTCAC GCTCATCGCA AAAGTCATCC AGAACCTGGC CAACTTCAAC
    TACAACAGGT TCAGCAGTAA AGAAGAATAT ATGTCCTTTA TGAATGAGTT CCTAGAGACG
    GAGTTGGCGA ATATGCAGAA TTTCCTGTAT GAGATCTCAA ACCTGGATTC GCTCACCAAC
    AGCATTAACT TTGAGGGCTA CATTGACCTT GGGCGGGAGC TGAGTACACT TCATGCCCTG
    CTTTGGGAAG TGATCCCACA ACTTAGTAAG GATGCACTTA TAAAGTTGGG GCCTCTTCCA
    CGCCTCCTTA ATGACATCAG CCTAGCTCTC AGGAACCCCC ACATCCAACG ACAACCAAGC
    CACCAAGAAC GACTGCCACC CCCCAAACCT GTCATTCTGA GGGGACCTTC AGCTGAGCTG
    CAAAGCTACT TGGTGCGAGA CCTCAACAGC TCTATTGACA TGACTCGCCT ACCCTCACCT
    ACTAAGGATA AACCATCAAA AGATATGTTT TTTGTTTCAC GTCGCCCGCT AACTCGCTCC
    TCGCCAGCCT ATTGCACCAG CAGCTCAGAC ATCACAGAAC CAGACCAGAA GATGCTGAGC
    GTGAACAAGA GTGTGTCCAT GATGGACTTG CAGGACAGCC GAATGAATAG CGTAACCAAC
    TTGCAGGCCG TAGGTGATCT GCTGAACTCC AGCCAGGCCT CCATATCAGG GGGCCTACGT
    CCGGGGGGCC GGCTCTCCCA GGGCAGTGGA TCCAGCATTG CAGCGGGTCT GAGGCTCAGT
    CAGATGGGGG TCACCACTGA TGGGCTCTCA GCTCAACAGC TACGCATTCC CTTGTCCTTC
    CAGAACCCGC TCTTCCACAT GGCTCCCGAT GGGCCTGGTG GGCCCCCATC TCATCCTGCT
    CCTCATCGAC CAGACCCCGG AGCTGATGCC TTTGCCCCTT TCCATGGGTA TAGCAAGAGT
    GAGGACTTGA GCTCCGGAAA GCATATGCTT CACAGTCACA GCTACAGCGA CGAATTCAGC
    CGGCAGGGTA AAAGGCAGTT ATCCCTCCAG GACAATCTCC AGCACATGCT TTCACCCCCT
    CAAATAACCA TTGGCCCTGG AGTGCCACGA AGGCAAGACT CACAGCCTCC CCCTTTGCAG
    AGGGGAAAAT CCCAACAACT GACGGTGCAG GCAGCGCATA AACCTCGGCC AAGCAGTGGC
    AACCTCCTAC AGAGCCCAGA ACCAATGTAT GGGGCAGCAA GAATTAGGCA GCAGTCAGTT
    GAAAAGGATG CAGGTGGCTT GAAACCATCA ATAACCAAGC AGCACTCCCA TGCACAGGCC
    ACTCTGAACC CTGTCACTCC AGCCTCAGAA CGCACAGTTG CCTGGGTATC CAACATGCCC
    CACCTTTCAG CAGATATTGA GAGCGCTCAC ATAGAACGGG AGGAGTATAA GTTGAAGGAA
    TACTCTAAAT CAATGGATGA GAGCCGTCTT GAGAGGGTGA AAGAGTATGA GGAAGAGATT
    TACTCCTTAA AAGAGAGGCT GTTGATGTCA AACCGGAAGC TGGAGGAATA TGAGAGGCGT
    CTTCTTACAC AGGAAGAACA AACTAATAAG ATGATGCAGC AATATCAGTC ACGCCTGGAC
    CAGAGTGAAA AGCGTTTGCG ACAGCAGCAG GTTGAGAAGG ACACGCAGAT AAAGAGCATC
    TTAAACAGGT TGATGCTGGT GGAGGAGGAG ATCAGGAAGG ATCACTCACA AGAACCAATC
    GATATGAAGA GGAGACTTAT CGAGGCTCAG GAGAAGAAGT TTCCCTCCGT GGGTACAGCA
    GACCCGCGTC TGAGGCTGGC GACTCCATGG AATGGGGCCC TCCCGCCTCT GCCTCCTCGA
    CTCCAAATCA CAGAAAATGG AGACTTTCTA AACTCAAGTG ACCATCACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa46374
    Clone ID Related Accession (Same CDS sequence) XM_018096731.1
    Accession Version XM_018096731.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3651bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ras/Rap GTPase-activating protein SynGAP isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGATGTTCT ATGCTCCCTT CAGAGATGCT CGAGGACCTC CCATGCATCG TATGCCCTAT 
    GTCACCTCTC CATACGACCG TCCAGCCTGG AACCCAAGAT ACTGTGTCCT CTCTGGAAAT
    CAGCTTCTCA TGCTTGATGA GGAGGAGGTT CACCCGCTGT TGATACGTGA AAGGAGAACT
    GATCCAACCA GAACAAAGTT CCTAAGGCGC ACAGTCAGTG TCCCCGTGGA GGGGAAGCAG
    CCTGACGAAC ACGATTATCA CCTTGCACGT TCCCGGAGGA AAAGCGTCCC AGGGGGAAAA
    CAGCCCAGTA TGGACGCCCC TCCCACCGCC CCCTTTCGAC CGTCGCAAGG ATTCCTGAGC
    CGTCGCTTGA AGAGCTCTAT AAAGAGGACA AAGAGTCAGC CCAAATTAGA TAGGACCAGC
    AGTTTCCGGC AAATATTGCC AAGGTTTCGA AGTGCTGATC ATGATAGGGC ACGGCTCATG
    CAGGCCTTTA AAGAGTCACA CTCTCACGAG TCCTTGCTTA GTCCTAGCAG TGCTGCAGAG
    GCTTTGGATT TGAACTTGGA TGAGGATTCT GTGGTGAAGC CTGTACACAG CAGCATCTTG
    GGACAGGAGT TTTGCTTCGA GGTGACCACA TCATCAGGTA CCAAATGTTT TGCCTGCCGT
    TCTGCTGCTG AAAGAGACAA ATGGATTGAG AATCTTCAAA GAGCTGTAAA GCCCAATAAG
    GATAACAGCA GGCGGGTAGA TAATGTCTTA AAGCTGTGGA TTATTGAAGC CCGTGATCTT
    CCCCCCAAGA AGCGCTATTA TTGTGAGCTC TGTTTGGATG ACATTTTGTA TGCCCGTACC
    ACCAGCAAGC CTCGGAATGA CACTGTCTTT TGGGGGGAAC ACTTTGAATT TAATAACCTT
    CCAGCTGTTC GGAACTTGCG CTTGCACCTC TATAAGGACA CAGACAAGAA AAGGCGCAAA
    GAAAAGAGCA ACTATGTGGG CATGGTGAAC GTGCCTATTG CCAGCATTAG TGGGCGCCAC
    TTTGTGGAGC AGTGGTACCC GGTTTCTCAG CCCACATCCT ACAAGGGGAA GAGCAGCTGT
    CCCTCCATTC GCCTAAAATC CCGATACCAA ACCATGAGCA TCCTCCCCAT GGAGCTGTAC
    AAGGAGTTTG CAGAGTATGT CACCAACAAC TACCGGATGC TGTGCGCTGT ACTGGAGCCT
    GTGCTTAGCG TGAAGAGCAA AGAGGAGGTT GCCTGTGCTT TAGTGCATAT ACTGCAAAGC
    ACAGGCAAGG CCAAGGACTT CCTTTCAGAC ATGGCTATGA CCGAGGTTGA TCGCTTCCTT
    GACAGAGAGC ACCTTATTCT CCGTGAGAAC ACACTTGCAA CTAAAGCCAT AGAGGAGTAT
    CTGAAACTGA TAGGACAGAG ATACCTCAAG GAAGCCATTG GTGAGTTTAT TCGGGCACTG
    TATGAGTCTG AGGAGGACTG TGAGGTTGAT CCCATGCGTT GTCCACCCTC TGCATTGCCC
    GATCACCAGG CCAATCTCAG GATGTGCTGT GAACTCGCCC TTTGCAAAGT CTTTAACTCT
    CATTGTGTGT TTCCTCGGGA GTTGAAGGAA GTCTTTGCAT CTTGGAGGAT GCGCTGTGCA
    GAGCGCGGCC GGGAAGATAT AGCAGATCGA CTCATCAGTG CCTCTCTCTT CCTCCGATTC
    CTGTGTCCGG CCATTATGTC CCCCAGTCTC TTCAGCCTAA TGCAGGAATA CCCTGATGAG
    CAAACATCTA GGACCCTCAC GCTCATCGCA AAAGTCATCC AGAACCTGGC CAACTTCAAC
    TACAACAGGT TCAGCAGTAA AGAAGAATAT ATGTCCTTTA TGAATGAGTT CCTAGAGACG
    GAGTTGGCGA ATATGCAGAA TTTCCTGTAT GAGATCTCAA ACCTGGATTC GCTCACCAAC
    AGCATTAACT TTGAGGGCTA CATTGACCTT GGGCGGGAGC TGAGTACACT TCATGCCCTG
    CTTTGGGAAG TGATCCCACA ACTTAGTAAG GATGCACTTA TAAAGTTGGG GCCTCTTCCA
    CGCCTCCTTA ATGACATCAG CCTAGCTCTC AGGAACCCCC ACATCCAACG ACAACCAAGC
    CACCAAGAAC GACTGCCACC CCCCAAACCT GTCATTCTGA GGGGACCTTC AGCTGAGCTG
    CAAAGCTACT TGGTGCGAGA CCTCAACAGC TCTATTGACA TGACTCGCCT ACCCTCACCT
    ACTAAGGATA AACCATCAAA AGATATGTTT TTTGTTTCAC GTCGCCCGCT AACTCGCTCC
    TCGCCAGCCT ATTGCACCAG CAGCTCAGAC ATCACAGAAC CAGACCAGAA GATGCTGAGC
    GTGAACAAGA GTGTGTCCAT GATGGACTTG CAGGACAGCC GAATGAATAG CGTAACCAAC
    TTGCAGGCCG TAGGTGATCT GCTGAACTCC AGCCAGGCCT CCATATCAGG GGGCCTACGT
    CCGGGGGGCC GGCTCTCCCA GGGCAGTGGA TCCAGCATTG CAGCGGGTCT GAGGCTCAGT
    CAGATGGGGG TCACCACTGA TGGGCTCTCA GCTCAACAGC TACGCATTCC CTTGTCCTTC
    CAGAACCCGC TCTTCCACAT GGCTCCCGAT GGGCCTGGTG GGCCCCCATC TCATCCTGCT
    CCTCATCGAC CAGACCCCGG AGCTGATGCC TTTGCCCCTT TCCATGGGTA TAGCAAGAGT
    GAGGACTTGA GCTCCGGAAA GCATATGCTT CACAGTCACA GCTACAGCGA CGAATTCAGC
    CGGCAGGGTA AAAGGCAGTT ATCCCTCCAG GACAATCTCC AGCACATGCT TTCACCCCCT
    CAAATAACCA TTGGCCCTGG AGTGCCACGA AGGCAAGACT CACAGCCTCC CCCTTTGCAG
    AGGGGAAAAT CCCAACAACT GACGGTGCAG GCAGCGCATA AACCTCGGCC AAGCAGTGGC
    AACCTCCTAC AGAGCCCAGA ACCAATGTAT GGGGCAGCAA GAATTAGGCA GCAGTCAGTT
    GAAAAGGATG CAGGTGGCTT GAAACCATCA ATAACCAAGC AGCACTCCCA TGCACAGGCC
    ACTCTGAACC CTGTCACTCC AGCCTCAGAA CGCACAGTTG CCTGGGTATC CAACATGCCC
    CACCTTTCAG CAGATATTGA GAGCGCTCAC ATAGAACGGG AGGAGTATAA GTTGAAGGAA
    TACTCTAAAT CAATGGATGA GAGCCGTCTT GAGAGGGTGA AAGAGTATGA GGAAGAGATT
    TACTCCTTAA AAGAGAGGCT GTTGATGTCA AACCGGAAGC TGGAGGAATA TGAGAGGCGT
    CTTCTTACAC AGGAAGAACA AACTAATAAG ATGATGCAGC AATATCAGTC ACGCCTGGAC
    CAGAGTGAAA AGCGTTTGCG ACAGCAGCAG GTTGAGAAGG ACACGCAGAT AAAGAGCATC
    TTAAACAGGT TAGAAATGGT TGATGCTGGT GGAGGAGGAG ATCAGGAAGG ATCACTCACA
    AGAACCAATC GATATGAAGA GGAGACTTAT CGAGGCTCAG GAGAAGAAGT TTCCCTCCGT
    GGGTACAGCA GACCCGCGTC TGAGGCTGGC GACTCCATGG AATGGGGCCC TCCCGCCTCT
    GCCTCCTCGA CTCCAAATCA CAGAAAATGG AGACTTTCTA AACTCAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017952220.1
    CDS942..4592
    Translation

    Target ORF information:

    RefSeq Version XM_018096731.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018096731.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGATGTTCT ATGCTCCCTT CAGAGATGCT CGAGGACCTC CCATGCATCG TATGCCCTAT 
    GTCACCTCTC CATACGACCG TCCAGCCTGG AACCCAAGAT ACTGTGTCCT CTCTGGAAAT
    CAGCTTCTCA TGCTTGATGA GGAGGAGGTT CACCCGCTGT TGATACGTGA AAGGAGAACT
    GATCCAACCA GAACAAAGTT CCTAAGGCGC ACAGTCAGTG TCCCCGTGGA GGGGAAGCAG
    CCTGACGAAC ACGATTATCA CCTTGCACGT TCCCGGAGGA AAAGCGTCCC AGGGGGAAAA
    CAGCCCAGTA TGGACGCCCC TCCCACCGCC CCCTTTCGAC CGTCGCAAGG ATTCCTGAGC
    CGTCGCTTGA AGAGCTCTAT AAAGAGGACA AAGAGTCAGC CCAAATTAGA TAGGACCAGC
    AGTTTCCGGC AAATATTGCC AAGGTTTCGA AGTGCTGATC ATGATAGGGC ACGGCTCATG
    CAGGCCTTTA AAGAGTCACA CTCTCACGAG TCCTTGCTTA GTCCTAGCAG TGCTGCAGAG
    GCTTTGGATT TGAACTTGGA TGAGGATTCT GTGGTGAAGC CTGTACACAG CAGCATCTTG
    GGACAGGAGT TTTGCTTCGA GGTGACCACA TCATCAGGTA CCAAATGTTT TGCCTGCCGT
    TCTGCTGCTG AAAGAGACAA ATGGATTGAG AATCTTCAAA GAGCTGTAAA GCCCAATAAG
    GATAACAGCA GGCGGGTAGA TAATGTCTTA AAGCTGTGGA TTATTGAAGC CCGTGATCTT
    CCCCCCAAGA AGCGCTATTA TTGTGAGCTC TGTTTGGATG ACATTTTGTA TGCCCGTACC
    ACCAGCAAGC CTCGGAATGA CACTGTCTTT TGGGGGGAAC ACTTTGAATT TAATAACCTT
    CCAGCTGTTC GGAACTTGCG CTTGCACCTC TATAAGGACA CAGACAAGAA AAGGCGCAAA
    GAAAAGAGCA ACTATGTGGG CATGGTGAAC GTGCCTATTG CCAGCATTAG TGGGCGCCAC
    TTTGTGGAGC AGTGGTACCC GGTTTCTCAG CCCACATCCT ACAAGGGGAA GAGCAGCTGT
    CCCTCCATTC GCCTAAAATC CCGATACCAA ACCATGAGCA TCCTCCCCAT GGAGCTGTAC
    AAGGAGTTTG CAGAGTATGT CACCAACAAC TACCGGATGC TGTGCGCTGT ACTGGAGCCT
    GTGCTTAGCG TGAAGAGCAA AGAGGAGGTT GCCTGTGCTT TAGTGCATAT ACTGCAAAGC
    ACAGGCAAGG CCAAGGACTT CCTTTCAGAC ATGGCTATGA CCGAGGTTGA TCGCTTCCTT
    GACAGAGAGC ACCTTATTCT CCGTGAGAAC ACACTTGCAA CTAAAGCCAT AGAGGAGTAT
    CTGAAACTGA TAGGACAGAG ATACCTCAAG GAAGCCATTG GTGAGTTTAT TCGGGCACTG
    TATGAGTCTG AGGAGGACTG TGAGGTTGAT CCCATGCGTT GTCCACCCTC TGCATTGCCC
    GATCACCAGG CCAATCTCAG GATGTGCTGT GAACTCGCCC TTTGCAAAGT CTTTAACTCT
    CATTGTGTGT TTCCTCGGGA GTTGAAGGAA GTCTTTGCAT CTTGGAGGAT GCGCTGTGCA
    GAGCGCGGCC GGGAAGATAT AGCAGATCGA CTCATCAGTG CCTCTCTCTT CCTCCGATTC
    CTGTGTCCGG CCATTATGTC CCCCAGTCTC TTCAGCCTAA TGCAGGAATA CCCTGATGAG
    CAAACATCTA GGACCCTCAC GCTCATCGCA AAAGTCATCC AGAACCTGGC CAACTTCAAC
    TACAACAGGT TCAGCAGTAA AGAAGAATAT ATGTCCTTTA TGAATGAGTT CCTAGAGACG
    GAGTTGGCGA ATATGCAGAA TTTCCTGTAT GAGATCTCAA ACCTGGATTC GCTCACCAAC
    AGCATTAACT TTGAGGGCTA CATTGACCTT GGGCGGGAGC TGAGTACACT TCATGCCCTG
    CTTTGGGAAG TGATCCCACA ACTTAGTAAG GATGCACTTA TAAAGTTGGG GCCTCTTCCA
    CGCCTCCTTA ATGACATCAG CCTAGCTCTC AGGAACCCCC ACATCCAACG ACAACCAAGC
    CACCAAGAAC GACTGCCACC CCCCAAACCT GTCATTCTGA GGGGACCTTC AGCTGAGCTG
    CAAAGCTACT TGGTGCGAGA CCTCAACAGC TCTATTGACA TGACTCGCCT ACCCTCACCT
    ACTAAGGATA AACCATCAAA AGATATGTTT TTTGTTTCAC GTCGCCCGCT AACTCGCTCC
    TCGCCAGCCT ATTGCACCAG CAGCTCAGAC ATCACAGAAC CAGACCAGAA GATGCTGAGC
    GTGAACAAGA GTGTGTCCAT GATGGACTTG CAGGACAGCC GAATGAATAG CGTAACCAAC
    TTGCAGGCCG TAGGTGATCT GCTGAACTCC AGCCAGGCCT CCATATCAGG GGGCCTACGT
    CCGGGGGGCC GGCTCTCCCA GGGCAGTGGA TCCAGCATTG CAGCGGGTCT GAGGCTCAGT
    CAGATGGGGG TCACCACTGA TGGGCTCTCA GCTCAACAGC TACGCATTCC CTTGTCCTTC
    CAGAACCCGC TCTTCCACAT GGCTCCCGAT GGGCCTGGTG GGCCCCCATC TCATCCTGCT
    CCTCATCGAC CAGACCCCGG AGCTGATGCC TTTGCCCCTT TCCATGGGTA TAGCAAGAGT
    GAGGACTTGA GCTCCGGAAA GCATATGCTT CACAGTCACA GCTACAGCGA CGAATTCAGC
    CGGCAGGGTA AAAGGCAGTT ATCCCTCCAG GACAATCTCC AGCACATGCT TTCACCCCCT
    CAAATAACCA TTGGCCCTGG AGTGCCACGA AGGCAAGACT CACAGCCTCC CCCTTTGCAG
    AGGGGAAAAT CCCAACAACT GACGGTGCAG GCAGCGCATA AACCTCGGCC AAGCAGTGGC
    AACCTCCTAC AGAGCCCAGA ACCAATGTAT GGGGCAGCAA GAATTAGGCA GCAGTCAGTT
    GAAAAGGATG CAGGTGGCTT GAAACCATCA ATAACCAAGC AGCACTCCCA TGCACAGGCC
    ACTCTGAACC CTGTCACTCC AGCCTCAGAA CGCACAGTTG CCTGGGTATC CAACATGCCC
    CACCTTTCAG CAGATATTGA GAGCGCTCAC ATAGAACGGG AGGAGTATAA GTTGAAGGAA
    TACTCTAAAT CAATGGATGA GAGCCGTCTT GAGAGGGTGA AAGAGTATGA GGAAGAGATT
    TACTCCTTAA AAGAGAGGCT GTTGATGTCA AACCGGAAGC TGGAGGAATA TGAGAGGCGT
    CTTCTTACAC AGGAAGAACA AACTAATAAG ATGATGCAGC AATATCAGTC ACGCCTGGAC
    CAGAGTGAAA AGCGTTTGCG ACAGCAGCAG GTTGAGAAGG ACACGCAGAT AAAGAGCATC
    TTAAACAGGT TAGAAATGGT TGATGCTGGT GGAGGAGGAG ATCAGGAAGG ATCACTCACA
    AGAACCAATC GATATGAAGA GGAGACTTAT CGAGGCTCAG GAGAAGAAGT TTCCCTCCGT
    GGGTACAGCA GACCCGCGTC TGAGGCTGGC GACTCCATGG AATGGGGCCC TCCCGCCTCT
    GCCTCCTCGA CTCCAAATCA CAGAAAATGG AGACTTTCTA AACTCAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa46375
    Clone ID Related Accession (Same CDS sequence) XM_018096732.1
    Accession Version XM_018096732.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3534bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ras/Rap GTPase-activating protein SynGAP isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGATGTTCT ATGCTCCCTT CAGAGATGCT CGAGGACCTC CCATGCATCG TATGCCCTAT 
    GTCACCTCTC CATACGACCG TCCAGCCTGG AACCCAAGAT ACTGTGTCCT CTCTGGAAAT
    CAGCTTCTCA TGCTTGATGA GGAGGAGGTT CACCCGCTGT TGATACGTGA AAGGAGAACT
    GATCCAACCA GAACAAAGTT CCTAAGGCGC ACAGTCAGTG TCCCCGTGGA GGGGAAGCAG
    CCTGACGAAC ACGATTATCA CCTTGCACGT TCCCGGAGGA AAAGCGTCCC AGGGGGAAAA
    CAGCCCAGTA TGGACGCCCC TCCCACCGCC CCCTTTCGAC CGTCGCAAGG ATTCCTGAGC
    CGTCGCTTGA AGAGCTCTAT AAAGAGGACA AAGAGTCAGC CCAAATTAGA TAGGACCAGC
    AGTTTCCGGC AAATATTGCC AAGGTTTCGA AGTGCTGATC ATGATAGGGC ACGGCTCATG
    CAGGCCTTTA AAGAGTCACA CTCTCACGAG TCCTTGCTTA GTCCTAGCAG TGCTGCAGAG
    GCTTTGGATT TGAACTTGGA TGAGGATTCT GTGGTGAAGC CTGTACACAG CAGCATCTTG
    GGACAGGAGT TTTGCTTCGA GGTGACCACA TCATCAGGTA CCAAATGTTT TGCCTGCCGT
    TCTGCTGCTG AAAGAGACAA ATGGATTGAG AATCTTCAAA GAGCTGTAAA GCCCAATAAG
    GATAACAGCA GGCGGGTAGA TAATGTCTTA AAGCTGTGGA TTATTGAAGC CCGTGATCTT
    CCCCCCAAGA AGCGCTATTA TTGTGAGCTC TGTTTGGATG ACATTTTGTA TGCCCGTACC
    ACCAGCAAGC CTCGGAATGA CACTGTCTTT TGGGGGGAAC ACTTTGAATT TAATAACCTT
    CCAGCTGTTC GGAACTTGCG CTTGCACCTC TATAAGGACA CAGACAAGAA AAGGCGCAAA
    GAAAAGAGCA ACTATGTGGG CATGGTGAAC GTGCCTATTG CCAGCATTAG TGGGCGCCAC
    TTTGTGGAGC AGTGGTACCC GGTTTCTCAG CCCACATCCT ACAAGGGGAA GAGCAGCTGT
    CCCTCCATTC GCCTAAAATC CCGATACCAA ACCATGAGCA TCCTCCCCAT GGAGCTGTAC
    AAGGAGTTTG CAGAGTATGT CACCAACAAC TACCGGATGC TGTGCGCTGT ACTGGAGCCT
    GTGCTTAGCG TGAAGAGCAA AGAGGAGGTT GCCTGTGCTT TAGTGCATAT ACTGCAAAGC
    ACAGGCAAGG CCAAGGACTT CCTTTCAGAC ATGGCTATGA CCGAGGTTGA TCGCTTCCTT
    GACAGAGAGC ACCTTATTCT CCGTGAGAAC ACACTTGCAA CTAAAGCCAT AGAGGAGTAT
    CTGAAACTGA TAGGACAGAG ATACCTCAAG GAAGCCATTG GTGAGTTTAT TCGGGCACTG
    TATGAGTCTG AGGAGGACTG TGAGGTTGAT CCCATGCGTT GTCCACCCTC TGCATTGCCC
    GATCACCAGG CCAATCTCAG GATGTGCTGT GAACTCGCCC TTTGCAAAGT CTTTAACTCT
    CATTGTGTGT TTCCTCGGGA GTTGAAGGAA GTCTTTGCAT CTTGGAGGAT GCGCTGTGCA
    GAGCGCGGCC GGGAAGATAT AGCAGATCGA CTCATCAGTG CCTCTCTCTT CCTCCGATTC
    CTGTGTCCGG CCATTATGTC CCCCAGTCTC TTCAGCCTAA TGCAGGAATA CCCTGATGAG
    CAAACATCTA GGACCCTCAC GCTCATCGCA AAAGTCATCC AGAACCTGGC CAACTTCAAC
    TACAACAGGT TCAGCAGTAA AGAAGAATAT ATGTCCTTTA TGAATGAGTT CCTAGAGACG
    GAGTTGGCGA ATATGCAGAA TTTCCTGTAT GAGATCTCAA ACCTGGATTC GCTCACCAAC
    AGCATTAACT TTGAGGGCTA CATTGACCTT GGGCGGGAGC TGAGTACACT TCATGCCCTG
    CTTTGGGAAG TGATCCCACA ACTTAGTAAG GATGCACTTA TAAAGTTGGG GCCTCTTCCA
    CGCCTCCTTA ATGACATCAG CCTAGCTCTC AGGAACCCCC ACATCCAACG ACAACCAAGC
    CACCAAGAAC GACTGCCACC CCCCAAACCT GTCATTCTGA GGGGACCTTC AGCTGAGCTG
    CAAAGCTACT TGGTGCGAGA CCTCAACAGC TCTATTGACA TGACTCGCCT ACCCTCACCT
    ACTAAGGATA AACCATCAAA AGATATGTTT TTTGTTTCAC GTCGCCCGCT AACTCGCTCC
    TCGCCAGCCT ATTGCACCAG CAGCTCAGAC ATCACAGAAC CAGACCAGAA GATGCTGAGC
    GTGAACAAGA GTGTGTCCAT GATGGACTTG CAGGACAGCC GAATGAATAG CGTAACCAAC
    TTGCAGGCCG TAGGTGATCT GCTGAACTCC AGCCAGGCCT CCATATCAGG GGGCCTACGT
    CCGGGGGGCC GGCTCTCCCA GGGCAGTGGA TCCAGCATTG CAGCGGGTCT GAGGCTCAGT
    CAGATGGGGG TCACCACTGA TGGGCTCTCA GCTCAACAGC TACGCATTCC CTTGTCCTTC
    CAGAACCCGC TCTTCCACAT GGCTCCCGAT GGGCCTGGTG GGCCCCCATC TCATCCTGCT
    CCTCATCGAC CAGACCCCGG AGCTGATGCC TTTGCCCCTT TCCATGGGTA TAGCAAGAGT
    GAGGACTTGA GCTCCGGAAA GCATATGCTT CACAGTCACA GCTACAGCGA CGAATTCAGC
    CGGCAGGGTA AAAGGCAGTT ATCCCTCCAG GACAATCTCC AGCACATGCT TTCACCCCCT
    CAAATAACCA TTGGCCCTGG AGTGCCACGA AGGCAAGACT CACAGCCTCC CCCTTTGCAG
    AGGGGAAAAT CCCAACAACT GACGGTGCAG GCAGCGCATA AACCTCGGCC AAGCAGTGGC
    AACCTCCTAC AGAGCCCAGA ACCAATGTAT GGGGCAGCAA GAATTAGGCA GCAGTCAGTT
    GAAAAGGATG CAGGTGGCTT GAAACCATCA ATAACCAAGC AGCACTCCCA TGCACAGGCC
    ACTCTGAACC CTGTCACTCC AGCCTCAGAA CGCACAGTTG CCTGGGTATC CAACATGCCC
    CACCTTTCAG CAGATATTGA GAGCGCTCAC ATAGAACGGG AGGAGTATAA GTTGAAGGAA
    TACTCTAAAT CAATGGATGA GAGCCGTCTT GAGAGGGTGA AAGAGTATGA GGAAGAGATT
    TACTCCTTAA AAGAGAGGCT GTTGATGTCA AACCGGAAGC TGGAGGAATA TGAGAGGCGT
    CTTCTTACAC AGGAAGAACA AACTAATAAG ATGATGCAGC AATATCAGTC ACGCCTGGAC
    CAGAGTGAAA AGCGTTTGCG ACAGCAGCAG GTTGAGAAGG ACACGCAGAT AAAGAGCATC
    TTAAACAGGT TAGAAATGGT TGATGCTGGT GGAGGAGGAG ATCAGGAAGG ATCACTCACA
    AGAACCAATC GATATGAAGA GGAGACTTAT CGAGGCTCAG GTCTGAACTG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017952221.1
    CDS941..4474
    Translation

    Target ORF information:

    RefSeq Version XM_018096732.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018096732.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGATGTTCT ATGCTCCCTT CAGAGATGCT CGAGGACCTC CCATGCATCG TATGCCCTAT 
    GTCACCTCTC CATACGACCG TCCAGCCTGG AACCCAAGAT ACTGTGTCCT CTCTGGAAAT
    CAGCTTCTCA TGCTTGATGA GGAGGAGGTT CACCCGCTGT TGATACGTGA AAGGAGAACT
    GATCCAACCA GAACAAAGTT CCTAAGGCGC ACAGTCAGTG TCCCCGTGGA GGGGAAGCAG
    CCTGACGAAC ACGATTATCA CCTTGCACGT TCCCGGAGGA AAAGCGTCCC AGGGGGAAAA
    CAGCCCAGTA TGGACGCCCC TCCCACCGCC CCCTTTCGAC CGTCGCAAGG ATTCCTGAGC
    CGTCGCTTGA AGAGCTCTAT AAAGAGGACA AAGAGTCAGC CCAAATTAGA TAGGACCAGC
    AGTTTCCGGC AAATATTGCC AAGGTTTCGA AGTGCTGATC ATGATAGGGC ACGGCTCATG
    CAGGCCTTTA AAGAGTCACA CTCTCACGAG TCCTTGCTTA GTCCTAGCAG TGCTGCAGAG
    GCTTTGGATT TGAACTTGGA TGAGGATTCT GTGGTGAAGC CTGTACACAG CAGCATCTTG
    GGACAGGAGT TTTGCTTCGA GGTGACCACA TCATCAGGTA CCAAATGTTT TGCCTGCCGT
    TCTGCTGCTG AAAGAGACAA ATGGATTGAG AATCTTCAAA GAGCTGTAAA GCCCAATAAG
    GATAACAGCA GGCGGGTAGA TAATGTCTTA AAGCTGTGGA TTATTGAAGC CCGTGATCTT
    CCCCCCAAGA AGCGCTATTA TTGTGAGCTC TGTTTGGATG ACATTTTGTA TGCCCGTACC
    ACCAGCAAGC CTCGGAATGA CACTGTCTTT TGGGGGGAAC ACTTTGAATT TAATAACCTT
    CCAGCTGTTC GGAACTTGCG CTTGCACCTC TATAAGGACA CAGACAAGAA AAGGCGCAAA
    GAAAAGAGCA ACTATGTGGG CATGGTGAAC GTGCCTATTG CCAGCATTAG TGGGCGCCAC
    TTTGTGGAGC AGTGGTACCC GGTTTCTCAG CCCACATCCT ACAAGGGGAA GAGCAGCTGT
    CCCTCCATTC GCCTAAAATC CCGATACCAA ACCATGAGCA TCCTCCCCAT GGAGCTGTAC
    AAGGAGTTTG CAGAGTATGT CACCAACAAC TACCGGATGC TGTGCGCTGT ACTGGAGCCT
    GTGCTTAGCG TGAAGAGCAA AGAGGAGGTT GCCTGTGCTT TAGTGCATAT ACTGCAAAGC
    ACAGGCAAGG CCAAGGACTT CCTTTCAGAC ATGGCTATGA CCGAGGTTGA TCGCTTCCTT
    GACAGAGAGC ACCTTATTCT CCGTGAGAAC ACACTTGCAA CTAAAGCCAT AGAGGAGTAT
    CTGAAACTGA TAGGACAGAG ATACCTCAAG GAAGCCATTG GTGAGTTTAT TCGGGCACTG
    TATGAGTCTG AGGAGGACTG TGAGGTTGAT CCCATGCGTT GTCCACCCTC TGCATTGCCC
    GATCACCAGG CCAATCTCAG GATGTGCTGT GAACTCGCCC TTTGCAAAGT CTTTAACTCT
    CATTGTGTGT TTCCTCGGGA GTTGAAGGAA GTCTTTGCAT CTTGGAGGAT GCGCTGTGCA
    GAGCGCGGCC GGGAAGATAT AGCAGATCGA CTCATCAGTG CCTCTCTCTT CCTCCGATTC
    CTGTGTCCGG CCATTATGTC CCCCAGTCTC TTCAGCCTAA TGCAGGAATA CCCTGATGAG
    CAAACATCTA GGACCCTCAC GCTCATCGCA AAAGTCATCC AGAACCTGGC CAACTTCAAC
    TACAACAGGT TCAGCAGTAA AGAAGAATAT ATGTCCTTTA TGAATGAGTT CCTAGAGACG
    GAGTTGGCGA ATATGCAGAA TTTCCTGTAT GAGATCTCAA ACCTGGATTC GCTCACCAAC
    AGCATTAACT TTGAGGGCTA CATTGACCTT GGGCGGGAGC TGAGTACACT TCATGCCCTG
    CTTTGGGAAG TGATCCCACA ACTTAGTAAG GATGCACTTA TAAAGTTGGG GCCTCTTCCA
    CGCCTCCTTA ATGACATCAG CCTAGCTCTC AGGAACCCCC ACATCCAACG ACAACCAAGC
    CACCAAGAAC GACTGCCACC CCCCAAACCT GTCATTCTGA GGGGACCTTC AGCTGAGCTG
    CAAAGCTACT TGGTGCGAGA CCTCAACAGC TCTATTGACA TGACTCGCCT ACCCTCACCT
    ACTAAGGATA AACCATCAAA AGATATGTTT TTTGTTTCAC GTCGCCCGCT AACTCGCTCC
    TCGCCAGCCT ATTGCACCAG CAGCTCAGAC ATCACAGAAC CAGACCAGAA GATGCTGAGC
    GTGAACAAGA GTGTGTCCAT GATGGACTTG CAGGACAGCC GAATGAATAG CGTAACCAAC
    TTGCAGGCCG TAGGTGATCT GCTGAACTCC AGCCAGGCCT CCATATCAGG GGGCCTACGT
    CCGGGGGGCC GGCTCTCCCA GGGCAGTGGA TCCAGCATTG CAGCGGGTCT GAGGCTCAGT
    CAGATGGGGG TCACCACTGA TGGGCTCTCA GCTCAACAGC TACGCATTCC CTTGTCCTTC
    CAGAACCCGC TCTTCCACAT GGCTCCCGAT GGGCCTGGTG GGCCCCCATC TCATCCTGCT
    CCTCATCGAC CAGACCCCGG AGCTGATGCC TTTGCCCCTT TCCATGGGTA TAGCAAGAGT
    GAGGACTTGA GCTCCGGAAA GCATATGCTT CACAGTCACA GCTACAGCGA CGAATTCAGC
    CGGCAGGGTA AAAGGCAGTT ATCCCTCCAG GACAATCTCC AGCACATGCT TTCACCCCCT
    CAAATAACCA TTGGCCCTGG AGTGCCACGA AGGCAAGACT CACAGCCTCC CCCTTTGCAG
    AGGGGAAAAT CCCAACAACT GACGGTGCAG GCAGCGCATA AACCTCGGCC AAGCAGTGGC
    AACCTCCTAC AGAGCCCAGA ACCAATGTAT GGGGCAGCAA GAATTAGGCA GCAGTCAGTT
    GAAAAGGATG CAGGTGGCTT GAAACCATCA ATAACCAAGC AGCACTCCCA TGCACAGGCC
    ACTCTGAACC CTGTCACTCC AGCCTCAGAA CGCACAGTTG CCTGGGTATC CAACATGCCC
    CACCTTTCAG CAGATATTGA GAGCGCTCAC ATAGAACGGG AGGAGTATAA GTTGAAGGAA
    TACTCTAAAT CAATGGATGA GAGCCGTCTT GAGAGGGTGA AAGAGTATGA GGAAGAGATT
    TACTCCTTAA AAGAGAGGCT GTTGATGTCA AACCGGAAGC TGGAGGAATA TGAGAGGCGT
    CTTCTTACAC AGGAAGAACA AACTAATAAG ATGATGCAGC AATATCAGTC ACGCCTGGAC
    CAGAGTGAAA AGCGTTTGCG ACAGCAGCAG GTTGAGAAGG ACACGCAGAT AAAGAGCATC
    TTAAACAGGT TAGAAATGGT TGATGCTGGT GGAGGAGGAG ATCAGGAAGG ATCACTCACA
    AGAACCAATC GATATGAAGA GGAGACTTAT CGAGGCTCAG GTCTGAACTG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa46376
    Clone ID Related Accession (Same CDS sequence) XM_018096733.1
    Accession Version XM_018096733.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3528bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ras/Rap GTPase-activating protein SynGAP isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGATGTTCT ATGCTCCCTT CAGAGATGCT CGAGGACCTC CCATGCATCG TATGCCCTAT 
    GTCACCTCTC CATACGACCG TCCAGCCTGG AACCCAAGAT ACTGTGTCCT CTCTGGAAAT
    CAGCTTCTCA TGCTTGATGA GGAGGAGGTT CACCCGCTGT TGATACGTGA AAGGAGAACT
    GATCCAACCA GAACAAAGTT CCTAAGGCGC ACAGTCAGTG TCCCCGTGGA GGGGAAGCAG
    CCTGACGAAC ACGATTATCA CCTTGCACGT TCCCGGAGGA AAAGCGTCCC AGGGGGAAAA
    CAGCCCAGTA TGGACGCCCC TCCCACCGCC CCCTTTCGAC CGTCGCAAGG ATTCCTGAGC
    CGTCGCTTGA AGAGCTCTAT AAAGAGGACA AAGAGTCAGC CCAAATTAGA TAGGACCAGC
    AGTTTCCGGC AAATATTGCC AAGGTTTCGA AGTGCTGATC ATGATAGGGC ACGGCTCATG
    CAGGCCTTTA AAGAGTCACA CTCTCACGAG TCCTTGCTTA GTCCTAGCAG TGCTGCAGAG
    GCTTTGGATT TGAACTTGGA TGAGGATTCT GTGGTGAAGC CTGTACACAG CAGCATCTTG
    GGACAGGAGT TTTGCTTCGA GGTGACCACA TCATCAGGTA CCAAATGTTT TGCCTGCCGT
    TCTGCTGCTG AAAGAGACAA ATGGATTGAG AATCTTCAAA GAGCTGTAAA GCCCAATAAG
    GATAACAGCA GGCGGGTAGA TAATGTCTTA AAGCTGTGGA TTATTGAAGC CCGTGATCTT
    CCCCCCAAGA AGCGCTATTA TTGTGAGCTC TGTTTGGATG ACATTTTGTA TGCCCGTACC
    ACCAGCAAGC CTCGGAATGA CACTGTCTTT TGGGGGGAAC ACTTTGAATT TAATAACCTT
    CCAGCTGTTC GGAACTTGCG CTTGCACCTC TATAAGGACA CAGACAAGAA AAGGCGCAAA
    GAAAAGAGCA ACTATGTGGG CATGGTGAAC GTGCCTATTG CCAGCATTAG TGGGCGCCAC
    TTTGTGGAGC AGTGGTACCC GGTTTCTCAG CCCACATCCT ACAAGGGGAA GAGCAGCTGT
    CCCTCCATTC GCCTAAAATC CCGATACCAA ACCATGAGCA TCCTCCCCAT GGAGCTGTAC
    AAGGAGTTTG CAGAGTATGT CACCAACAAC TACCGGATGC TGTGCGCTGT ACTGGAGCCT
    GTGCTTAGCG TGAAGAGCAA AGAGGAGGTT GCCTGTGCTT TAGTGCATAT ACTGCAAAGC
    ACAGGCAAGG CCAAGGACTT CCTTTCAGAC ATGGCTATGA CCGAGGTTGA TCGCTTCCTT
    GACAGAGAGC ACCTTATTCT CCGTGAGAAC ACACTTGCAA CTAAAGCCAT AGAGGAGTAT
    CTGAAACTGA TAGGACAGAG ATACCTCAAG GAAGCCATTG GTGAGTTTAT TCGGGCACTG
    TATGAGTCTG AGGAGGACTG TGAGGTTGAT CCCATGCGTT GTCCACCCTC TGCATTGCCC
    GATCACCAGG CCAATCTCAG GATGTGCTGT GAACTCGCCC TTTGCAAAGT CTTTAACTCT
    CATTGTGTGT TTCCTCGGGA GTTGAAGGAA GTCTTTGCAT CTTGGAGGAT GCGCTGTGCA
    GAGCGCGGCC GGGAAGATAT AGCAGATCGA CTCATCAGTG CCTCTCTCTT CCTCCGATTC
    CTGTGTCCGG CCATTATGTC CCCCAGTCTC TTCAGCCTAA TGCAGGAATA CCCTGATGAG
    CAAACATCTA GGACCCTCAC GCTCATCGCA AAAGTCATCC AGAACCTGGC CAACTTCAAC
    TACAACAGGT TCAGCAGTAA AGAAGAATAT ATGTCCTTTA TGAATGAGTT CCTAGAGACG
    GAGTTGGCGA ATATGCAGAA TTTCCTGTAT GAGATCTCAA ACCTGGATTC GCTCACCAAC
    AGCATTAACT TTGAGGGCTA CATTGACCTT GGGCGGGAGC TGAGTACACT TCATGCCCTG
    CTTTGGGAAG TGATCCCACA ACTTAGTAAG GATGCACTTA TAAAGTTGGG GCCTCTTCCA
    CGCCTCCTTA ATGACATCAG CCTAGCTCTC AGGAACCCCC ACATCCAACG ACAACCAAGC
    CACCAAGAAC GACTGCCACC CCCCAAACCT GTCATTCTGA GGGGACCTTC AGCTGAGCTG
    CAAAGCTACT TGGTGCGAGA CCTCAACAGC TCTATTGACA TGACTCGCCT ACCCTCACCT
    ACTAAGGATA AACCATCAAA AGATATGTTT TTTGTTTCAC GTCGCCCGCT AACTCGCTCC
    TCGCCAGCCT ATTGCACCAG CAGCTCAGAC ATCACAGAAC CAGACCAGAA GATGCTGAGC
    GTGAACAAGA GTGTGTCCAT GATGGACTTG CAGGACAGCC GAATGAATAG CGTAACCAAC
    TTGCAGGCCA ACCCGCTCTT CCACATGGCT CCCGATGGGC CTGGTGGGCC CCCATCTCAT
    CCTGCTCCTC ATCGACCAGA CCCCGGAGCT GATGCCTTTG CCCCTTTCCA TGGGTATAGC
    AAGAGTGAGG ACTTGAGCTC CGGAAAGCAT ATGCTTCACA GTCACAGCTA CAGCGACGAA
    TTCAGCCGGC AGGGTAAAAG GCAGTTATCC CTCCAGGACA ATCTCCAGCA CATGCTTTCA
    CCCCCTCAAA TAACCATTGG CCCTGGAGTG CCACGAAGGC AAGACTCACA GCCTCCCCCT
    TTGCAGAGGG GAAAATCCCA ACAACTGACG GTGCAGGCAG CGCATAAACC TCGGCCAAGC
    AGTGGCAACC TCCTACAGAG CCCAGAACCA ATGTATGGGG CAGCAAGAAT TAGGCAGCAG
    TCAGTTGAAA AGGATGCAGG TGGCTTGAAA CCATCAATAA CCAAGCAGCA CTCCCATGCA
    CAGGCCACTC TGAACCCTGT CACTCCAGCC TCAGAACGCA CAGTTGCCTG GGTATCCAAC
    ATGCCCCACC TTTCAGCAGA TATTGAGAGC GCTCACATAG AACGGGAGGA GTATAAGTTG
    AAGGAATACT CTAAATCAAT GGATGAGAGC CGTCTTGAGA GGGTGAAAGA GTATGAGGAA
    GAGATTTACT CCTTAAAAGA GAGGCTGTTG ATGTCAAACC GGAAGCTGGA GGAATATGAG
    AGGCGTCTTC TTACACAGGA AGAACAAACT AATAAGATGA TGCAGCAATA TCAGTCACGC
    CTGGACCAGA GTGAAAAGCG TTTGCGACAG CAGCAGGTTG AGAAGGACAC GCAGATAAAG
    AGCATCTTAA ACAGGTTAGA AATGGTTGAT GCTGGTGGAG GAGGAGATCA GGAAGGATCA
    CTCACAAGAA CCAATCGATA TGAAGAGGAG ACTTATCGAG GCTCAGGTGG AAATTACAAT
    GTCATTGTGC GTTTGTGTGT GACATCTTCA TCACACCCTG TCCCTGTCTT TCATCTCAAG
    AGGGAGAGAA GAAAAAAAAA AACAAGGATA CAAAGAAATC TAGGGCTTAA TGAAGAAATA
    GGGGGGAGAA GAAGTTTCCC TCCGTGGGTA CAGCAGACCC GCGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017952222.1
    CDS945..4472
    Translation

    Target ORF information:

    RefSeq Version XM_018096733.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018096733.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGATGTTCT ATGCTCCCTT CAGAGATGCT CGAGGACCTC CCATGCATCG TATGCCCTAT 
    GTCACCTCTC CATACGACCG TCCAGCCTGG AACCCAAGAT ACTGTGTCCT CTCTGGAAAT
    CAGCTTCTCA TGCTTGATGA GGAGGAGGTT CACCCGCTGT TGATACGTGA AAGGAGAACT
    GATCCAACCA GAACAAAGTT CCTAAGGCGC ACAGTCAGTG TCCCCGTGGA GGGGAAGCAG
    CCTGACGAAC ACGATTATCA CCTTGCACGT TCCCGGAGGA AAAGCGTCCC AGGGGGAAAA
    CAGCCCAGTA TGGACGCCCC TCCCACCGCC CCCTTTCGAC CGTCGCAAGG ATTCCTGAGC
    CGTCGCTTGA AGAGCTCTAT AAAGAGGACA AAGAGTCAGC CCAAATTAGA TAGGACCAGC
    AGTTTCCGGC AAATATTGCC AAGGTTTCGA AGTGCTGATC ATGATAGGGC ACGGCTCATG
    CAGGCCTTTA AAGAGTCACA CTCTCACGAG TCCTTGCTTA GTCCTAGCAG TGCTGCAGAG
    GCTTTGGATT TGAACTTGGA TGAGGATTCT GTGGTGAAGC CTGTACACAG CAGCATCTTG
    GGACAGGAGT TTTGCTTCGA GGTGACCACA TCATCAGGTA CCAAATGTTT TGCCTGCCGT
    TCTGCTGCTG AAAGAGACAA ATGGATTGAG AATCTTCAAA GAGCTGTAAA GCCCAATAAG
    GATAACAGCA GGCGGGTAGA TAATGTCTTA AAGCTGTGGA TTATTGAAGC CCGTGATCTT
    CCCCCCAAGA AGCGCTATTA TTGTGAGCTC TGTTTGGATG ACATTTTGTA TGCCCGTACC
    ACCAGCAAGC CTCGGAATGA CACTGTCTTT TGGGGGGAAC ACTTTGAATT TAATAACCTT
    CCAGCTGTTC GGAACTTGCG CTTGCACCTC TATAAGGACA CAGACAAGAA AAGGCGCAAA
    GAAAAGAGCA ACTATGTGGG CATGGTGAAC GTGCCTATTG CCAGCATTAG TGGGCGCCAC
    TTTGTGGAGC AGTGGTACCC GGTTTCTCAG CCCACATCCT ACAAGGGGAA GAGCAGCTGT
    CCCTCCATTC GCCTAAAATC CCGATACCAA ACCATGAGCA TCCTCCCCAT GGAGCTGTAC
    AAGGAGTTTG CAGAGTATGT CACCAACAAC TACCGGATGC TGTGCGCTGT ACTGGAGCCT
    GTGCTTAGCG TGAAGAGCAA AGAGGAGGTT GCCTGTGCTT TAGTGCATAT ACTGCAAAGC
    ACAGGCAAGG CCAAGGACTT CCTTTCAGAC ATGGCTATGA CCGAGGTTGA TCGCTTCCTT
    GACAGAGAGC ACCTTATTCT CCGTGAGAAC ACACTTGCAA CTAAAGCCAT AGAGGAGTAT
    CTGAAACTGA TAGGACAGAG ATACCTCAAG GAAGCCATTG GTGAGTTTAT TCGGGCACTG
    TATGAGTCTG AGGAGGACTG TGAGGTTGAT CCCATGCGTT GTCCACCCTC TGCATTGCCC
    GATCACCAGG CCAATCTCAG GATGTGCTGT GAACTCGCCC TTTGCAAAGT CTTTAACTCT
    CATTGTGTGT TTCCTCGGGA GTTGAAGGAA GTCTTTGCAT CTTGGAGGAT GCGCTGTGCA
    GAGCGCGGCC GGGAAGATAT AGCAGATCGA CTCATCAGTG CCTCTCTCTT CCTCCGATTC
    CTGTGTCCGG CCATTATGTC CCCCAGTCTC TTCAGCCTAA TGCAGGAATA CCCTGATGAG
    CAAACATCTA GGACCCTCAC GCTCATCGCA AAAGTCATCC AGAACCTGGC CAACTTCAAC
    TACAACAGGT TCAGCAGTAA AGAAGAATAT ATGTCCTTTA TGAATGAGTT CCTAGAGACG
    GAGTTGGCGA ATATGCAGAA TTTCCTGTAT GAGATCTCAA ACCTGGATTC GCTCACCAAC
    AGCATTAACT TTGAGGGCTA CATTGACCTT GGGCGGGAGC TGAGTACACT TCATGCCCTG
    CTTTGGGAAG TGATCCCACA ACTTAGTAAG GATGCACTTA TAAAGTTGGG GCCTCTTCCA
    CGCCTCCTTA ATGACATCAG CCTAGCTCTC AGGAACCCCC ACATCCAACG ACAACCAAGC
    CACCAAGAAC GACTGCCACC CCCCAAACCT GTCATTCTGA GGGGACCTTC AGCTGAGCTG
    CAAAGCTACT TGGTGCGAGA CCTCAACAGC TCTATTGACA TGACTCGCCT ACCCTCACCT
    ACTAAGGATA AACCATCAAA AGATATGTTT TTTGTTTCAC GTCGCCCGCT AACTCGCTCC
    TCGCCAGCCT ATTGCACCAG CAGCTCAGAC ATCACAGAAC CAGACCAGAA GATGCTGAGC
    GTGAACAAGA GTGTGTCCAT GATGGACTTG CAGGACAGCC GAATGAATAG CGTAACCAAC
    TTGCAGGCCA ACCCGCTCTT CCACATGGCT CCCGATGGGC CTGGTGGGCC CCCATCTCAT
    CCTGCTCCTC ATCGACCAGA CCCCGGAGCT GATGCCTTTG CCCCTTTCCA TGGGTATAGC
    AAGAGTGAGG ACTTGAGCTC CGGAAAGCAT ATGCTTCACA GTCACAGCTA CAGCGACGAA
    TTCAGCCGGC AGGGTAAAAG GCAGTTATCC CTCCAGGACA ATCTCCAGCA CATGCTTTCA
    CCCCCTCAAA TAACCATTGG CCCTGGAGTG CCACGAAGGC AAGACTCACA GCCTCCCCCT
    TTGCAGAGGG GAAAATCCCA ACAACTGACG GTGCAGGCAG CGCATAAACC TCGGCCAAGC
    AGTGGCAACC TCCTACAGAG CCCAGAACCA ATGTATGGGG CAGCAAGAAT TAGGCAGCAG
    TCAGTTGAAA AGGATGCAGG TGGCTTGAAA CCATCAATAA CCAAGCAGCA CTCCCATGCA
    CAGGCCACTC TGAACCCTGT CACTCCAGCC TCAGAACGCA CAGTTGCCTG GGTATCCAAC
    ATGCCCCACC TTTCAGCAGA TATTGAGAGC GCTCACATAG AACGGGAGGA GTATAAGTTG
    AAGGAATACT CTAAATCAAT GGATGAGAGC CGTCTTGAGA GGGTGAAAGA GTATGAGGAA
    GAGATTTACT CCTTAAAAGA GAGGCTGTTG ATGTCAAACC GGAAGCTGGA GGAATATGAG
    AGGCGTCTTC TTACACAGGA AGAACAAACT AATAAGATGA TGCAGCAATA TCAGTCACGC
    CTGGACCAGA GTGAAAAGCG TTTGCGACAG CAGCAGGTTG AGAAGGACAC GCAGATAAAG
    AGCATCTTAA ACAGGTTAGA AATGGTTGAT GCTGGTGGAG GAGGAGATCA GGAAGGATCA
    CTCACAAGAA CCAATCGATA TGAAGAGGAG ACTTATCGAG GCTCAGGTGG AAATTACAAT
    GTCATTGTGC GTTTGTGTGT GACATCTTCA TCACACCCTG TCCCTGTCTT TCATCTCAAG
    AGGGAGAGAA GAAAAAAAAA AACAAGGATA CAAAGAAATC TAGGGCTTAA TGAAGAAATA
    GGGGGGAGAA GAAGTTTCCC TCCGTGGGTA CAGCAGACCC GCGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa46377
    Clone ID Related Accession (Same CDS sequence) XM_018096734.1
    Accession Version XM_018096734.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3525bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ras/Rap GTPase-activating protein SynGAP isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGATGTTCT ATGCTCCCTT CAGAGATGCT CGAGGACCTC CCATGCATCG TATGCCCTAT 
    GTCACCTCTC CATACGACCG TCCAGCCTGG AACCCAAGAT ACTGTGTCCT CTCTGGAAAT
    CAGCTTCTCA TGCTTGATGA GGAGGAGGTT CACCCGCTGT TGATACGTGA AAGGAGAACT
    GATCCAACCA GAACAAAGTT CCTAAGGCGC ACAGTCAGTG TCCCCGTGGA GGGGAAGCAG
    CCTGACGAAC ACGATTATCA CCTTGCACGT TCCCGGAGGA AAAGCGTCCC AGGGGGAAAA
    CAGCCCAGTA TGGACGCCCC TCCCACCGCC CCCTTTCGAC CGTCGCAAGG ATTCCTGAGC
    CGTCGCTTGA AGAGCTCTAT AAAGAGGACA AAGAGTCAGC CCAAATTAGA TAGGACCAGC
    AGTTTCCGGC AAATATTGCC AAGGTTTCGA AGTGCTGATC ATGATAGGGC ACGGCTCATG
    CAGGCCTTTA AAGAGTCACA CTCTCACGAG TCCTTGCTTA GTCCTAGCAG TGCTGCAGAG
    GCTTTGGATT TGAACTTGGA TGAGGATTCT GTGGTGAAGC CTGTACACAG CAGCATCTTG
    GGACAGGAGT TTTGCTTCGA GGTGACCACA TCATCAGGTA CCAAATGTTT TGCCTGCCGT
    TCTGCTGCTG AAAGAGACAA ATGGATTGAG AATCTTCAAA GAGCTGTAAA GCCCAATAAG
    GATAACAGCA GGCGGGTAGA TAATGTCTTA AAGCTGTGGA TTATTGAAGC CCGTGATCTT
    CCCCCCAAGA AGCGCTATTA TTGTGAGCTC TGTTTGGATG ACATTTTGTA TGCCCGTACC
    ACCAGCAAGC CTCGGAATGA CACTGTCTTT TGGGGGGAAC ACTTTGAATT TAATAACCTT
    CCAGCTGTTC GGAACTTGCG CTTGCACCTC TATAAGGACA CAGACAAGAA AAGGCGCAAA
    GAAAAGAGCA ACTATGTGGG CATGGTGAAC GTGCCTATTG CCAGCATTAG TGGGCGCCAC
    TTTGTGGAGC AGTGGTACCC GGTTTCTCAG CCCACATCCT ACAAGGGGAA GAGCAGCTGT
    CCCTCCATTC GCCTAAAATC CCGATACCAA ACCATGAGCA TCCTCCCCAT GGAGCTGTAC
    AAGGAGTTTG CAGAGTATGT CACCAACAAC TACCGGATGC TGTGCGCTGT ACTGGAGCCT
    GTGCTTAGCG TGAAGAGCAA AGAGGAGGTT GCCTGTGCTT TAGTGCATAT ACTGCAAAGC
    ACAGGCAAGG CCAAGGACTT CCTTTCAGAC ATGGCTATGA CCGAGGTTGA TCGCTTCCTT
    GACAGAGAGC ACCTTATTCT CCGTGAGAAC ACACTTGCAA CTAAAGCCAT AGAGGAGTAT
    CTGAAACTGA TAGGACAGAG ATACCTCAAG GAAGCCATTG GTGAGTTTAT TCGGGCACTG
    TATGAGTCTG AGGAGGACTG TGAGGTTGAT CCCATGCGTT GTCCACCCTC TGCATTGCCC
    GATCACCAGG CCAATCTCAG GATGTGCTGT GAACTCGCCC TTTGCAAAGT CTTTAACTCT
    CATTGTGTGT TTCCTCGGGA GTTGAAGGAA GTCTTTGCAT CTTGGAGGAT GCGCTGTGCA
    GAGCGCGGCC GGGAAGATAT AGCAGATCGA CTCATCAGTG CCTCTCTCTT CCTCCGATTC
    CTGTGTCCGG CCATTATGTC CCCCAGTCTC TTCAGCCTAA TGCAGGAATA CCCTGATGAG
    CAAACATCTA GGACCCTCAC GCTCATCGCA AAAGTCATCC AGAACCTGGC CAACTTCAAC
    TACAAGTTCA GCAGTAAAGA AGAATATATG TCCTTTATGA ATGAGTTCCT AGAGACGGAG
    TTGGCGAATA TGCAGAATTT CCTGTATGAG ATCTCAAACC TGGATTCGCT CACCAACAGC
    ATTAACTTTG AGGGCTACAT TGACCTTGGG CGGGAGCTGA GTACACTTCA TGCCCTGCTT
    TGGGAAGTGA TCCCACAACT TAGTAAGGAT GCACTTATAA AGTTGGGGCC TCTTCCACGC
    CTCCTTAATG ACATCAGCCT AGCTCTCAGG AACCCCCACA TCCAACGACA ACCAAGCCAC
    CAAGAACGAC TGCCACCCCC CAAACCTGTC ATTCTGAGGG GACCTTCAGC TGAGCTGCAA
    AGCTACTTGG TGCGAGACCT CAACAGCTCT ATTGACATGA CTCGCCTACC CTCACCTACT
    AAGGATAAAC CATCAAAAGA TATGTTTTTT GTTTCACGTC GCCCGCTAAC TCGCTCCTCG
    CCAGCCTATT GCACCAGCAG CTCAGACATC ACAGAACCAG ACCAGAAGAT GCTGAGCGTG
    AACAAGAGTG TGTCCATGAT GGACTTGCAG GACAGCCGAA TGAATAGCGT AACCAACTTG
    CAGGCCAACC CGCTCTTCCA CATGGCTCCC GATGGGCCTG GTGGGCCCCC ATCTCATCCT
    GCTCCTCATC GACCAGACCC CGGAGCTGAT GCCTTTGCCC CTTTCCATGG GTATAGCAAG
    AGTGAGGACT TGAGCTCCGG AAAGCATATG CTTCACAGTC ACAGCTACAG CGACGAATTC
    AGCCGGCAGG GTAAAAGGCA GTTATCCCTC CAGGACAATC TCCAGCACAT GCTTTCACCC
    CCTCAAATAA CCATTGGCCC TGGAGTGCCA CGAAGGCAAG ACTCACAGCC TCCCCCTTTG
    CAGAGGGGAA AATCCCAACA ACTGACGGTG CAGGCAGCGC ATAAACCTCG GCCAAGCAGT
    GGCAACCTCC TACAGAGCCC AGAACCAATG TATGGGGCAG CAAGAATTAG GCAGCAGTCA
    GTTGAAAAGG ATGCAGGTGG CTTGAAACCA TCAATAACCA AGCAGCACTC CCATGCACAG
    GCCACTCTGA ACCCTGTCAC TCCAGCCTCA GAACGCACAG TTGCCTGGGT ATCCAACATG
    CCCCACCTTT CAGCAGATAT TGAGAGCGCT CACATAGAAC GGGAGGAGTA TAAGTTGAAG
    GAATACTCTA AATCAATGGA TGAGAGCCGT CTTGAGAGGG TGAAAGAGTA TGAGGAAGAG
    ATTTACTCCT TAAAAGAGAG GCTGTTGATG TCAAACCGGA AGCTGGAGGA ATATGAGAGG
    CGTCTTCTTA CACAGGAAGA ACAAACTAAT AAGATGATGC AGCAATATCA GTCACGCCTG
    GACCAGAGTG AAAAGCGTTT GCGACAGCAG CAGGTTGAGA AGGACACGCA GATAAAGAGC
    ATCTTAAACA GGTTAGAAAT GGTTGATGCT GGTGGAGGAG GAGATCAGGA AGGATCACTC
    ACAAGAACCA ATCGATATGA AGAGGAGACT TATCGAGGCT CAGGTGGAAA TTACAATGTC
    ATTGTGCGTT TGTGTGTGAC ATCTTCATCA CACCCTGTCC CTGTCTTTCA TCTCAAGAGG
    GAGAGAAGAA AAAAAAAAAC AAGGATACAA AGAAATCTAG GGCTTAATGA AGAAATAGGG
    GGGAGAAGAA GTTTCCCTCC GTGGGTACAG CAGACCCGCG TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017952223.1
    CDS944..4468
    Translation

    Target ORF information:

    RefSeq Version XM_018096734.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018096734.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGATGTTCT ATGCTCCCTT CAGAGATGCT CGAGGACCTC CCATGCATCG TATGCCCTAT 
    GTCACCTCTC CATACGACCG TCCAGCCTGG AACCCAAGAT ACTGTGTCCT CTCTGGAAAT
    CAGCTTCTCA TGCTTGATGA GGAGGAGGTT CACCCGCTGT TGATACGTGA AAGGAGAACT
    GATCCAACCA GAACAAAGTT CCTAAGGCGC ACAGTCAGTG TCCCCGTGGA GGGGAAGCAG
    CCTGACGAAC ACGATTATCA CCTTGCACGT TCCCGGAGGA AAAGCGTCCC AGGGGGAAAA
    CAGCCCAGTA TGGACGCCCC TCCCACCGCC CCCTTTCGAC CGTCGCAAGG ATTCCTGAGC
    CGTCGCTTGA AGAGCTCTAT AAAGAGGACA AAGAGTCAGC CCAAATTAGA TAGGACCAGC
    AGTTTCCGGC AAATATTGCC AAGGTTTCGA AGTGCTGATC ATGATAGGGC ACGGCTCATG
    CAGGCCTTTA AAGAGTCACA CTCTCACGAG TCCTTGCTTA GTCCTAGCAG TGCTGCAGAG
    GCTTTGGATT TGAACTTGGA TGAGGATTCT GTGGTGAAGC CTGTACACAG CAGCATCTTG
    GGACAGGAGT TTTGCTTCGA GGTGACCACA TCATCAGGTA CCAAATGTTT TGCCTGCCGT
    TCTGCTGCTG AAAGAGACAA ATGGATTGAG AATCTTCAAA GAGCTGTAAA GCCCAATAAG
    GATAACAGCA GGCGGGTAGA TAATGTCTTA AAGCTGTGGA TTATTGAAGC CCGTGATCTT
    CCCCCCAAGA AGCGCTATTA TTGTGAGCTC TGTTTGGATG ACATTTTGTA TGCCCGTACC
    ACCAGCAAGC CTCGGAATGA CACTGTCTTT TGGGGGGAAC ACTTTGAATT TAATAACCTT
    CCAGCTGTTC GGAACTTGCG CTTGCACCTC TATAAGGACA CAGACAAGAA AAGGCGCAAA
    GAAAAGAGCA ACTATGTGGG CATGGTGAAC GTGCCTATTG CCAGCATTAG TGGGCGCCAC
    TTTGTGGAGC AGTGGTACCC GGTTTCTCAG CCCACATCCT ACAAGGGGAA GAGCAGCTGT
    CCCTCCATTC GCCTAAAATC CCGATACCAA ACCATGAGCA TCCTCCCCAT GGAGCTGTAC
    AAGGAGTTTG CAGAGTATGT CACCAACAAC TACCGGATGC TGTGCGCTGT ACTGGAGCCT
    GTGCTTAGCG TGAAGAGCAA AGAGGAGGTT GCCTGTGCTT TAGTGCATAT ACTGCAAAGC
    ACAGGCAAGG CCAAGGACTT CCTTTCAGAC ATGGCTATGA CCGAGGTTGA TCGCTTCCTT
    GACAGAGAGC ACCTTATTCT CCGTGAGAAC ACACTTGCAA CTAAAGCCAT AGAGGAGTAT
    CTGAAACTGA TAGGACAGAG ATACCTCAAG GAAGCCATTG GTGAGTTTAT TCGGGCACTG
    TATGAGTCTG AGGAGGACTG TGAGGTTGAT CCCATGCGTT GTCCACCCTC TGCATTGCCC
    GATCACCAGG CCAATCTCAG GATGTGCTGT GAACTCGCCC TTTGCAAAGT CTTTAACTCT
    CATTGTGTGT TTCCTCGGGA GTTGAAGGAA GTCTTTGCAT CTTGGAGGAT GCGCTGTGCA
    GAGCGCGGCC GGGAAGATAT AGCAGATCGA CTCATCAGTG CCTCTCTCTT CCTCCGATTC
    CTGTGTCCGG CCATTATGTC CCCCAGTCTC TTCAGCCTAA TGCAGGAATA CCCTGATGAG
    CAAACATCTA GGACCCTCAC GCTCATCGCA AAAGTCATCC AGAACCTGGC CAACTTCAAC
    TACAAGTTCA GCAGTAAAGA AGAATATATG TCCTTTATGA ATGAGTTCCT AGAGACGGAG
    TTGGCGAATA TGCAGAATTT CCTGTATGAG ATCTCAAACC TGGATTCGCT CACCAACAGC
    ATTAACTTTG AGGGCTACAT TGACCTTGGG CGGGAGCTGA GTACACTTCA TGCCCTGCTT
    TGGGAAGTGA TCCCACAACT TAGTAAGGAT GCACTTATAA AGTTGGGGCC TCTTCCACGC
    CTCCTTAATG ACATCAGCCT AGCTCTCAGG AACCCCCACA TCCAACGACA ACCAAGCCAC
    CAAGAACGAC TGCCACCCCC CAAACCTGTC ATTCTGAGGG GACCTTCAGC TGAGCTGCAA
    AGCTACTTGG TGCGAGACCT CAACAGCTCT ATTGACATGA CTCGCCTACC CTCACCTACT
    AAGGATAAAC CATCAAAAGA TATGTTTTTT GTTTCACGTC GCCCGCTAAC TCGCTCCTCG
    CCAGCCTATT GCACCAGCAG CTCAGACATC ACAGAACCAG ACCAGAAGAT GCTGAGCGTG
    AACAAGAGTG TGTCCATGAT GGACTTGCAG GACAGCCGAA TGAATAGCGT AACCAACTTG
    CAGGCCAACC CGCTCTTCCA CATGGCTCCC GATGGGCCTG GTGGGCCCCC ATCTCATCCT
    GCTCCTCATC GACCAGACCC CGGAGCTGAT GCCTTTGCCC CTTTCCATGG GTATAGCAAG
    AGTGAGGACT TGAGCTCCGG AAAGCATATG CTTCACAGTC ACAGCTACAG CGACGAATTC
    AGCCGGCAGG GTAAAAGGCA GTTATCCCTC CAGGACAATC TCCAGCACAT GCTTTCACCC
    CCTCAAATAA CCATTGGCCC TGGAGTGCCA CGAAGGCAAG ACTCACAGCC TCCCCCTTTG
    CAGAGGGGAA AATCCCAACA ACTGACGGTG CAGGCAGCGC ATAAACCTCG GCCAAGCAGT
    GGCAACCTCC TACAGAGCCC AGAACCAATG TATGGGGCAG CAAGAATTAG GCAGCAGTCA
    GTTGAAAAGG ATGCAGGTGG CTTGAAACCA TCAATAACCA AGCAGCACTC CCATGCACAG
    GCCACTCTGA ACCCTGTCAC TCCAGCCTCA GAACGCACAG TTGCCTGGGT ATCCAACATG
    CCCCACCTTT CAGCAGATAT TGAGAGCGCT CACATAGAAC GGGAGGAGTA TAAGTTGAAG
    GAATACTCTA AATCAATGGA TGAGAGCCGT CTTGAGAGGG TGAAAGAGTA TGAGGAAGAG
    ATTTACTCCT TAAAAGAGAG GCTGTTGATG TCAAACCGGA AGCTGGAGGA ATATGAGAGG
    CGTCTTCTTA CACAGGAAGA ACAAACTAAT AAGATGATGC AGCAATATCA GTCACGCCTG
    GACCAGAGTG AAAAGCGTTT GCGACAGCAG CAGGTTGAGA AGGACACGCA GATAAAGAGC
    ATCTTAAACA GGTTAGAAAT GGTTGATGCT GGTGGAGGAG GAGATCAGGA AGGATCACTC
    ACAAGAACCA ATCGATATGA AGAGGAGACT TATCGAGGCT CAGGTGGAAA TTACAATGTC
    ATTGTGCGTT TGTGTGTGAC ATCTTCATCA CACCCTGTCC CTGTCTTTCA TCTCAAGAGG
    GAGAGAAGAA AAAAAAAAAC AAGGATACAA AGAAATCTAG GGCTTAATGA AGAAATAGGG
    GGGAGAAGAA GTTTCCCTCC GTGGGTACAG CAGACCCGCG TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa46378
    Clone ID Related Accession (Same CDS sequence) XM_018096735.1
    Accession Version XM_018096735.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3516bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ras/Rap GTPase-activating protein SynGAP isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGATGTTCT ATGCTCCCTT CAGAGATGCT CGAGGACCTC CCATGCATCG TATGCCCTAT 
    GTCACCTCTC CATACGACCG TCCAGCCTGG AACCCAAGAT ACTGTGTCCT CTCTGGAAAT
    CAGCTTCTCA TGCTTGATGA GGAGGAGGTT CACCCGCTGT TGATACGTGA AAGGAGAACT
    GATCCAACCA GAACAAAGTT CCTAAGGCGC ACAGTCAGTG TCCCCGTGGA GGGGAAGCAG
    CCTGACGAAC ACGATTATCA CCTTGCACGT TCCCGGAGGA AAAGCGTCCC AGGGGGAAAA
    CAGCCCAGTA TGGACGCCCC TCCCACCGCC CCCTTTCGAC CGTCGCAAGG ATTCCTGAGC
    CGTCGCTTGA AGAGCTCTAT AAAGAGGACA AAGAGTCAGC CCAAATTAGA TAGGACCAGC
    AGTTTCCGGC AAATATTGCC AAGGTTTCGA AGTGCTGATC ATGATAGGGC ACGGCTCATG
    CAGGCCTTTA AAGAGTCACA CTCTCACGAG TCCTTGCTTA GTCCTAGCAG TGCTGCAGAG
    GCTTTGGATT TGAACTTGGA TGAGGATTCT GTGGTGAAGC CTGTACACAG CAGCATCTTG
    GGACAGGAGT TTTGCTTCGA GGTGACCACA TCATCAGGTA CCAAATGTTT TGCCTGCCGT
    TCTGCTGCTG AAAGAGACAA ATGGATTGAG AATCTTCAAA GAGCTGTAAA GCCCAATAAG
    GATAACAGCA GGCGGGTAGA TAATGTCTTA AAGCTGTGGA TTATTGAAGC CCGTGATCTT
    CCCCCCAAGA AGCGCTATTA TTGTGAGCTC TGTTTGGATG ACATTTTGTA TGCCCGTACC
    ACCAGCAAGC CTCGGAATGA CACTGTCTTT TGGGGGGAAC ACTTTGAATT TAATAACCTT
    CCAGCTGTTC GGAACTTGCG CTTGCACCTC TATAAGGACA CAGACAAGAA AAGGCGCAAA
    GAAAAGAGCA ACTATGTGGG CATGGTGAAC GTGCCTATTG CCAGCATTAG TGGGCGCCAC
    TTTGTGGAGC AGTGGTACCC GGTTTCTCAG CCCACATCCT ACAAGGGGAA GAGCAGCTGT
    CCCTCCATTC GCCTAAAATC CCGATACCAA ACCATGAGCA TCCTCCCCAT GGAGCTGTAC
    AAGGAGTTTG CAGAGTATGT CACCAACAAC TACCGGATGC TGTGCGCTGT ACTGGAGCCT
    GTGCTTAGCG TGAAGAGCAA AGAGGAGGTT GCCTGTGCTT TAGTGCATAT ACTGCAAAGC
    ACAGGCAAGG CCAAGGACTT CCTTTCAGAC ATGGCTATGA CCGAGGTTGA TCGCTTCCTT
    GACAGAGAGC ACCTTATTCT CCGTGAGAAC ACACTTGCAA CTAAAGCCAT AGAGGAGTAT
    CTGAAACTGA TAGGACAGAG ATACCTCAAG GAAGCCATTG GTGAGTTTAT TCGGGCACTG
    TATGAGTCTG AGGAGGACTG TGAGGTTGAT CCCATGCGTT GTCCACCCTC TGCATTGCCC
    GATCACCAGG CCAATCTCAG GATGTGCTGT GAACTCGCCC TTTGCAAAGT CTTTAACTCT
    CATTGTGTGT TTCCTCGGGA GTTGAAGGAA GTCTTTGCAT CTTGGAGGAT GCGCTGTGCA
    GAGCGCGGCC GGGAAGATAT AGCAGATCGA CTCATCAGTG CCTCTCTCTT CCTCCGATTC
    CTGTGTCCGG CCATTATGTC CCCCAGTCTC TTCAGCCTAA TGCAGGAATA CCCTGATGAG
    CAAACATCTA GGACCCTCAC GCTCATCGCA AAAGTCATCC AGAACCTGGC CAACTTCAAC
    TACAACAGGT TCAGCAGTAA AGAAGAATAT ATGTCCTTTA TGAATGAGTT CCTAGAGACG
    GAGTTGGCGA ATATGCAGAA TTTCCTGTAT GAGATCTCAA ACCTGGATTC GCTCACCAAC
    AGCATTAACT TTGAGGGCTA CATTGACCTT GGGCGGGAGC TGAGTACACT TCATGCCCTG
    CTTTGGGAAG TGATCCCACA ACTTAGTAAG GATGCACTTA TAAAGTTGGG GCCTCTTCCA
    CGCCTCCTTA ATGACATCAG CCTAGCTCTC AGGAACCCCC ACATCCAACG ACAACCAAGC
    CACCAAGAAC GACTGCCACC CCCCAAACCT GTCATTCTGA GGGGACCTTC AGCTGAGCTG
    CAAAGCTACT TGGTGCGAGA CCTCAACAGC TCTATTGACA TGACTCGCCT ACCCTCACCT
    ACTAAGGATA AACCATCAAA AGATATGTTT TTTGTTTCAC GTCGCCCGCT AACTCGCTCC
    TCGCCAGCCT ATTGCACCAG CAGCTCAGAC ATCACAGAAC CAGACCAGAA GATGCTGAGC
    GTGAACAAGA GTGTGTCCAT GATGGACTTG CAGGACAGCC GAATGAATAG CGTAACCAAC
    TTGCAGGCCG TAGGTGATCT GCTGAACTCC AGCCAGGCCT CCATATCAGG GGGCCTACGT
    CCGGGGGGCC GGCTCTCCCA GGGCAGTGGA TCCAGCATTG CAGCGGGTCT GAGGCTCAGT
    CAGATGGGGG TCACCACTGA TGGGCTCTCA GCTCAACAGC TACGCATTCC CTTGTCCTTC
    CAGAACCCGC TCTTCCACAT GGCTCCCGAT GGGCCTGGTG GGCCCCCATC TCATCCTGCT
    CCTCATCGAC CAGACCCCGG AGCTGATGCC TTTGCCCCTT TCCATGGGTA TAGCAAGAGT
    GAGGACTTGA GCTCCGGAAA GCATATGCTT CACAGTCACA GCTACAGCGA CGAATTCAGC
    CGGCAGGGTA AAAGGCAGTT ATCCCTCCAG GACAATCTCC AGCACATGCT TTCACCCCCT
    CAAATAACCA TTGGCCCTGG AGTGCCACGA AGGCAAGACT CACAGCCTCC CCCTTTGCAG
    AGGGGAAAAT CCCAACAACT GACGGTGCAG GCAGCGCATA AACCTCGGCC AAGCAGTGGC
    AACCTCCTAC AGAGCCCAGA ACCAATGTAT GGGGCAGCAA GAATTAGGCA GCAGTCAGTT
    GAAAAGGATG CAGGTGGCTT GAAACCATCA ATAACCAAGC AGCACTCCCA TGCACAGGCC
    ACTCTGAACC CTGTCACTCC AGCCTCAGAA CGCACAGTTG CCTGGGTATC CAACATGCCC
    CACCTTTCAG CAGATATTGA GAGCGCTCAC ATAGAACGGG AGGAGTATAA GTTGAAGGAA
    TACTCTAAAT CAATGGATGA GAGCCGTCTT GAGAGGGTGA AAGAGTATGA GGAAGAGATT
    TACTCCTTAA AAGAGAGGCT GTTGATGTCA AACCGGAAGC TGGAGGAATA TGAGAGGCGT
    CTTCTTACAC AGGAAGAACA AACTAATAAG ATGATGCAGC AATATCAGTC ACGCCTGGAC
    CAGAGTGAAA AGCGTTTGCG ACAGCAGCAG GTTGAGAAGG ACACGCAGAT AAAGAGCATC
    TTAAACAGGT TGATGCTGGT GGAGGAGGAG ATCAGGAAGG ATCACTCACA AGAACCAATC
    GATATGAAGA GGAGACTTAT CGAGGCTCAG GTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017952224.1
    CDS940..4455
    Translation

    Target ORF information:

    RefSeq Version XM_018096735.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018096735.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGATGTTCT ATGCTCCCTT CAGAGATGCT CGAGGACCTC CCATGCATCG TATGCCCTAT 
    GTCACCTCTC CATACGACCG TCCAGCCTGG AACCCAAGAT ACTGTGTCCT CTCTGGAAAT
    CAGCTTCTCA TGCTTGATGA GGAGGAGGTT CACCCGCTGT TGATACGTGA AAGGAGAACT
    GATCCAACCA GAACAAAGTT CCTAAGGCGC ACAGTCAGTG TCCCCGTGGA GGGGAAGCAG
    CCTGACGAAC ACGATTATCA CCTTGCACGT TCCCGGAGGA AAAGCGTCCC AGGGGGAAAA
    CAGCCCAGTA TGGACGCCCC TCCCACCGCC CCCTTTCGAC CGTCGCAAGG ATTCCTGAGC
    CGTCGCTTGA AGAGCTCTAT AAAGAGGACA AAGAGTCAGC CCAAATTAGA TAGGACCAGC
    AGTTTCCGGC AAATATTGCC AAGGTTTCGA AGTGCTGATC ATGATAGGGC ACGGCTCATG
    CAGGCCTTTA AAGAGTCACA CTCTCACGAG TCCTTGCTTA GTCCTAGCAG TGCTGCAGAG
    GCTTTGGATT TGAACTTGGA TGAGGATTCT GTGGTGAAGC CTGTACACAG CAGCATCTTG
    GGACAGGAGT TTTGCTTCGA GGTGACCACA TCATCAGGTA CCAAATGTTT TGCCTGCCGT
    TCTGCTGCTG AAAGAGACAA ATGGATTGAG AATCTTCAAA GAGCTGTAAA GCCCAATAAG
    GATAACAGCA GGCGGGTAGA TAATGTCTTA AAGCTGTGGA TTATTGAAGC CCGTGATCTT
    CCCCCCAAGA AGCGCTATTA TTGTGAGCTC TGTTTGGATG ACATTTTGTA TGCCCGTACC
    ACCAGCAAGC CTCGGAATGA CACTGTCTTT TGGGGGGAAC ACTTTGAATT TAATAACCTT
    CCAGCTGTTC GGAACTTGCG CTTGCACCTC TATAAGGACA CAGACAAGAA AAGGCGCAAA
    GAAAAGAGCA ACTATGTGGG CATGGTGAAC GTGCCTATTG CCAGCATTAG TGGGCGCCAC
    TTTGTGGAGC AGTGGTACCC GGTTTCTCAG CCCACATCCT ACAAGGGGAA GAGCAGCTGT
    CCCTCCATTC GCCTAAAATC CCGATACCAA ACCATGAGCA TCCTCCCCAT GGAGCTGTAC
    AAGGAGTTTG CAGAGTATGT CACCAACAAC TACCGGATGC TGTGCGCTGT ACTGGAGCCT
    GTGCTTAGCG TGAAGAGCAA AGAGGAGGTT GCCTGTGCTT TAGTGCATAT ACTGCAAAGC
    ACAGGCAAGG CCAAGGACTT CCTTTCAGAC ATGGCTATGA CCGAGGTTGA TCGCTTCCTT
    GACAGAGAGC ACCTTATTCT CCGTGAGAAC ACACTTGCAA CTAAAGCCAT AGAGGAGTAT
    CTGAAACTGA TAGGACAGAG ATACCTCAAG GAAGCCATTG GTGAGTTTAT TCGGGCACTG
    TATGAGTCTG AGGAGGACTG TGAGGTTGAT CCCATGCGTT GTCCACCCTC TGCATTGCCC
    GATCACCAGG CCAATCTCAG GATGTGCTGT GAACTCGCCC TTTGCAAAGT CTTTAACTCT
    CATTGTGTGT TTCCTCGGGA GTTGAAGGAA GTCTTTGCAT CTTGGAGGAT GCGCTGTGCA
    GAGCGCGGCC GGGAAGATAT AGCAGATCGA CTCATCAGTG CCTCTCTCTT CCTCCGATTC
    CTGTGTCCGG CCATTATGTC CCCCAGTCTC TTCAGCCTAA TGCAGGAATA CCCTGATGAG
    CAAACATCTA GGACCCTCAC GCTCATCGCA AAAGTCATCC AGAACCTGGC CAACTTCAAC
    TACAACAGGT TCAGCAGTAA AGAAGAATAT ATGTCCTTTA TGAATGAGTT CCTAGAGACG
    GAGTTGGCGA ATATGCAGAA TTTCCTGTAT GAGATCTCAA ACCTGGATTC GCTCACCAAC
    AGCATTAACT TTGAGGGCTA CATTGACCTT GGGCGGGAGC TGAGTACACT TCATGCCCTG
    CTTTGGGAAG TGATCCCACA ACTTAGTAAG GATGCACTTA TAAAGTTGGG GCCTCTTCCA
    CGCCTCCTTA ATGACATCAG CCTAGCTCTC AGGAACCCCC ACATCCAACG ACAACCAAGC
    CACCAAGAAC GACTGCCACC CCCCAAACCT GTCATTCTGA GGGGACCTTC AGCTGAGCTG
    CAAAGCTACT TGGTGCGAGA CCTCAACAGC TCTATTGACA TGACTCGCCT ACCCTCACCT
    ACTAAGGATA AACCATCAAA AGATATGTTT TTTGTTTCAC GTCGCCCGCT AACTCGCTCC
    TCGCCAGCCT ATTGCACCAG CAGCTCAGAC ATCACAGAAC CAGACCAGAA GATGCTGAGC
    GTGAACAAGA GTGTGTCCAT GATGGACTTG CAGGACAGCC GAATGAATAG CGTAACCAAC
    TTGCAGGCCG TAGGTGATCT GCTGAACTCC AGCCAGGCCT CCATATCAGG GGGCCTACGT
    CCGGGGGGCC GGCTCTCCCA GGGCAGTGGA TCCAGCATTG CAGCGGGTCT GAGGCTCAGT
    CAGATGGGGG TCACCACTGA TGGGCTCTCA GCTCAACAGC TACGCATTCC CTTGTCCTTC
    CAGAACCCGC TCTTCCACAT GGCTCCCGAT GGGCCTGGTG GGCCCCCATC TCATCCTGCT
    CCTCATCGAC CAGACCCCGG AGCTGATGCC TTTGCCCCTT TCCATGGGTA TAGCAAGAGT
    GAGGACTTGA GCTCCGGAAA GCATATGCTT CACAGTCACA GCTACAGCGA CGAATTCAGC
    CGGCAGGGTA AAAGGCAGTT ATCCCTCCAG GACAATCTCC AGCACATGCT TTCACCCCCT
    CAAATAACCA TTGGCCCTGG AGTGCCACGA AGGCAAGACT CACAGCCTCC CCCTTTGCAG
    AGGGGAAAAT CCCAACAACT GACGGTGCAG GCAGCGCATA AACCTCGGCC AAGCAGTGGC
    AACCTCCTAC AGAGCCCAGA ACCAATGTAT GGGGCAGCAA GAATTAGGCA GCAGTCAGTT
    GAAAAGGATG CAGGTGGCTT GAAACCATCA ATAACCAAGC AGCACTCCCA TGCACAGGCC
    ACTCTGAACC CTGTCACTCC AGCCTCAGAA CGCACAGTTG CCTGGGTATC CAACATGCCC
    CACCTTTCAG CAGATATTGA GAGCGCTCAC ATAGAACGGG AGGAGTATAA GTTGAAGGAA
    TACTCTAAAT CAATGGATGA GAGCCGTCTT GAGAGGGTGA AAGAGTATGA GGAAGAGATT
    TACTCCTTAA AAGAGAGGCT GTTGATGTCA AACCGGAAGC TGGAGGAATA TGAGAGGCGT
    CTTCTTACAC AGGAAGAACA AACTAATAAG ATGATGCAGC AATATCAGTC ACGCCTGGAC
    CAGAGTGAAA AGCGTTTGCG ACAGCAGCAG GTTGAGAAGG ACACGCAGAT AAAGAGCATC
    TTAAACAGGT TGATGCTGGT GGAGGAGGAG ATCAGGAAGG ATCACTCACA AGAACCAATC
    GATATGAAGA GGAGACTTAT CGAGGCTCAG GTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa46379
    Clone ID Related Accession (Same CDS sequence) XM_018096736.1
    Accession Version XM_018096736.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3432bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ras/Rap GTPase-activating protein SynGAP isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGATGTTCT ATGCTCCCTT CAGAGATGCT CGAGGACCTC CCATGCATCG TATGCCCTAT 
    GTCACCTCTC CATACGACCG TCCAGCCTGG AACCCAAGAT ACTGTGTCCT CTCTGGAAAT
    CAGCTTCTCA TGCTTGATGA GGAGGAGGTT CACCCGCTGT TGATACGTGA AAGGAGAACT
    GATCCAACCA GAACAAAGTT CCTAAGGCGC ACAGTCAGTG TCCCCGTGGA GGGGAAGCAG
    CCTGACGAAC ACGATTATCA CCTTGCACGT TCCCGGAGGA AAAGCGTCCC AGGGGGAAAA
    CAGCCCAGTA TGGACGCCCC TCCCACCGCC CCCTTTCGAC CGTCGCAAGG ATTCCTGAGC
    CGTCGCTTGA AGAGCTCTAT AAAGAGGACA AAGAGTCAGC CCAAATTAGA TAGGACCAGC
    AGTTTCCGGC AAATATTGCC AAGGTTTCGA AGTGCTGATC ATGATAGGGC ACGGCTCATG
    CAGGCCTTTA AAGAGTCACA CTCTCACGAG TCCTTGCTTA GTCCTAGCAG TGCTGCAGAG
    GCTTTGGATT TGAACTTGGA TGAGGATTCT GTGGTGAAGC CTGTACACAG CAGCATCTTG
    GGACAGGAGT TTTGCTTCGA GGTGACCACA TCATCAGGTA CCAAATGTTT TGCCTGCCGT
    TCTGCTGCTG AAAGAGACAA ATGGATTGAG AATCTTCAAA GAGCTGTAAA GCCCAATAAG
    GATAACAGCA GGCGGGTAGA TAATGTCTTA AAGCTGTGGA TTATTGAAGC CCGTGATCTT
    CCCCCCAAGA AGCGCTATTA TTGTGAGCTC TGTTTGGATG ACATTTTGTA TGCCCGTACC
    ACCAGCAAGC CTCGGAATGA CACTGTCTTT TGGGGGGAAC ACTTTGAATT TAATAACCTT
    CCAGCTGTTC GGAACTTGCG CTTGCACCTC TATAAGGACA CAGACAAGAA AAGGCGCAAA
    GAAAAGAGCA ACTATGTGGG CATGGTGAAC GTGCCTATTG CCAGCATTAG TGGGCGCCAC
    TTTGTGGAGC AGTGGTACCC GGTTTCTCAG CCCACATCCT ACAAGGGGAA GAGCAGCTGT
    CCCTCCATTC GCCTAAAATC CCGATACCAA ACCATGAGCA TCCTCCCCAT GGAGCTGTAC
    AAGGAGTTTG CAGAGTATGT CACCAACAAC TACCGGATGC TGTGCGCTGT ACTGGAGCCT
    GTGCTTAGCG TGAAGAGCAA AGAGGAGGTT GCCTGTGCTT TAGTGCATAT ACTGCAAAGC
    ACAGGCAAGG CCAAGGACTT CCTTTCAGAC ATGGCTATGA CCGAGGTTGA TCGCTTCCTT
    GACAGAGAGC ACCTTATTCT CCGTGAGAAC ACACTTGCAA CTAAAGCCAT AGAGGAGTAT
    CTGAAACTGA TAGGACAGAG ATACCTCAAG GAAGCCATTG GTGAGTTTAT TCGGGCACTG
    TATGAGTCTG AGGAGGACTG TGAGGTTGAT CCCATGCGTT GTCCACCCTC TGCATTGCCC
    GATCACCAGG CCAATCTCAG GATGTGCTGT GAACTCGCCC TTTGCAAAGT CTTTAACTCT
    CATTGTGTGT TTCCTCGGGA GTTGAAGGAA GTCTTTGCAT CTTGGAGGAT GCGCTGTGCA
    GAGCGCGGCC GGGAAGATAT AGCAGATCGA CTCATCAGTG CCTCTCTCTT CCTCCGATTC
    CTGTGTCCGG CCATTATGTC CCCCAGTCTC TTCAGCCTAA TGCAGGAATA CCCTGATGAG
    CAAACATCTA GGACCCTCAC GCTCATCGCA AAAGTCATCC AGAACCTGGC CAACTTCAAC
    TACAACAGGT TCAGCAGTAA AGAAGAATAT ATGTCCTTTA TGAATGAGTT CCTAGAGACG
    GAGTTGGCGA ATATGCAGAA TTTCCTGTAT GAGATCTCAA ACCTGGATTC GCTCACCAAC
    AGCATTAACT TTGAGGGCTA CATTGACCTT GGGCGGGAGC TGAGTACACT TCATGCCCTG
    CTTTGGGAAG TGATCCCACA ACTTAGTAAG GATGCACTTA TAAAGTTGGG GCCTCTTCCA
    CGCCTCCTTA ATGACATCAG CCTAGCTCTC AGGAACCCCC ACATCCAACG ACAACCAAGC
    CACCAAGAAC GACTGCCACC CCCCAAACCT GTCATTCTGA GGGGACCTTC AGCTGAGCTG
    CAAAGCTACT TGGTGCGAGA CCTCAACAGC TCTATTGACA TGACTCGCCT ACCCTCACCT
    ACTAAGGATA AACCATCAAA AGATATGTTT TTTGTTTCAC GTCGCCCGCT AACTCGCTCC
    TCGCCAGCCT ATTGCACCAG CAGCTCAGAC ATCACAGAAC CAGACCAGAA GATGCTGAGC
    GTGAACAAGA GTGTGTCCAT GATGGACTTG CAGGACAGCC GAATGAATAG CGTAACCAAC
    TTGCAGGCCG TAGGTGATCT GCTGAACTCC AGCCAGGCCT CCATATCAGG GGGCCTACGT
    CCGGGGGGCC GGCTCTCCCA GGGCAGTGGA TCCAGCATTG CAGCGGGTCT GAGGCTCAGT
    CAGATGGGGG TCACCACTGA TGGGCTCTCA GCTCAACAGC TACGCATTCC CTTGTCCTTC
    CAGAACCCGC TCTTCCACAT GGCTCCCGAT GGGCCTGGTG GGCCCCCATC TCATCCTGCT
    CCTCATCGAC CAGACCCCGG AGCTGATGCC TTTGCCCCTT TCCATGGGTA TAGCAAGAGT
    GAGGACTTGA GCTCCGGAAA GCATATGCTT CACAGTCACA GCTACAGCGA CGAATTCAGC
    CGGCAGGGTA AAAGGCAGTT ATCCCTCCAG GACAATCTCC AGCACATGCT TTCACCCCCT
    CAAATAACCA TTGGCCCTGG AGTGCCACGA AGGCAAGACT CACAGCCTCC CCCTTTGCAG
    AGGGGAAAAT CCCAACAACT GACGGTGCAG GCAGCGCATA AACCTCGGCC AAGCAGTGGC
    AACCTCCTAC AGAGCCCAGA ACCAATGTAT GGGGCAGCAA GAATTAGGCA GCAGTCAGTT
    GAAAAGGATG CAGGTGGCTT GAAACCATCA ATAACCAAGC AGCACTCCCA TGCACAGGCC
    ACTCTGAACC CTGTCACTCC AGCCTCAGAA CGCACAGTTG CCTGGGTATC CAACATGCCC
    CACCTTTCAG CAGATATTGA GAGCGCTCAC ATAGAACGGG AGGAGTATAA GTTGAAGGAA
    TACTCTAAAT CAATGGATGA GAGCCGTCTT GAGAGGGTGA AAGAGTATGA GGAAGAGATT
    TACTCCTTAA AAGAGAGGCT GTTGATGTCA AACCGGAAGC TGGAGGAATA TGAGAGGCGT
    CTTCTTACAC AGGAAGAACA AACTAATAAG ATGATGCAGC AATATCAGTC ACGCCTGGAC
    CAGAGTGAAA AGCGTTTGCG ACAGCAGCAG GTTGAGAAGG ACACGCAGAT AAAGAGCATC
    TTAAACAGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017952225.1
    CDS939..4370
    Translation

    Target ORF information:

    RefSeq Version XM_018096736.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018096736.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGATGTTCT ATGCTCCCTT CAGAGATGCT CGAGGACCTC CCATGCATCG TATGCCCTAT 
    GTCACCTCTC CATACGACCG TCCAGCCTGG AACCCAAGAT ACTGTGTCCT CTCTGGAAAT
    CAGCTTCTCA TGCTTGATGA GGAGGAGGTT CACCCGCTGT TGATACGTGA AAGGAGAACT
    GATCCAACCA GAACAAAGTT CCTAAGGCGC ACAGTCAGTG TCCCCGTGGA GGGGAAGCAG
    CCTGACGAAC ACGATTATCA CCTTGCACGT TCCCGGAGGA AAAGCGTCCC AGGGGGAAAA
    CAGCCCAGTA TGGACGCCCC TCCCACCGCC CCCTTTCGAC CGTCGCAAGG ATTCCTGAGC
    CGTCGCTTGA AGAGCTCTAT AAAGAGGACA AAGAGTCAGC CCAAATTAGA TAGGACCAGC
    AGTTTCCGGC AAATATTGCC AAGGTTTCGA AGTGCTGATC ATGATAGGGC ACGGCTCATG
    CAGGCCTTTA AAGAGTCACA CTCTCACGAG TCCTTGCTTA GTCCTAGCAG TGCTGCAGAG
    GCTTTGGATT TGAACTTGGA TGAGGATTCT GTGGTGAAGC CTGTACACAG CAGCATCTTG
    GGACAGGAGT TTTGCTTCGA GGTGACCACA TCATCAGGTA CCAAATGTTT TGCCTGCCGT
    TCTGCTGCTG AAAGAGACAA ATGGATTGAG AATCTTCAAA GAGCTGTAAA GCCCAATAAG
    GATAACAGCA GGCGGGTAGA TAATGTCTTA AAGCTGTGGA TTATTGAAGC CCGTGATCTT
    CCCCCCAAGA AGCGCTATTA TTGTGAGCTC TGTTTGGATG ACATTTTGTA TGCCCGTACC
    ACCAGCAAGC CTCGGAATGA CACTGTCTTT TGGGGGGAAC ACTTTGAATT TAATAACCTT
    CCAGCTGTTC GGAACTTGCG CTTGCACCTC TATAAGGACA CAGACAAGAA AAGGCGCAAA
    GAAAAGAGCA ACTATGTGGG CATGGTGAAC GTGCCTATTG CCAGCATTAG TGGGCGCCAC
    TTTGTGGAGC AGTGGTACCC GGTTTCTCAG CCCACATCCT ACAAGGGGAA GAGCAGCTGT
    CCCTCCATTC GCCTAAAATC CCGATACCAA ACCATGAGCA TCCTCCCCAT GGAGCTGTAC
    AAGGAGTTTG CAGAGTATGT CACCAACAAC TACCGGATGC TGTGCGCTGT ACTGGAGCCT
    GTGCTTAGCG TGAAGAGCAA AGAGGAGGTT GCCTGTGCTT TAGTGCATAT ACTGCAAAGC
    ACAGGCAAGG CCAAGGACTT CCTTTCAGAC ATGGCTATGA CCGAGGTTGA TCGCTTCCTT
    GACAGAGAGC ACCTTATTCT CCGTGAGAAC ACACTTGCAA CTAAAGCCAT AGAGGAGTAT
    CTGAAACTGA TAGGACAGAG ATACCTCAAG GAAGCCATTG GTGAGTTTAT TCGGGCACTG
    TATGAGTCTG AGGAGGACTG TGAGGTTGAT CCCATGCGTT GTCCACCCTC TGCATTGCCC
    GATCACCAGG CCAATCTCAG GATGTGCTGT GAACTCGCCC TTTGCAAAGT CTTTAACTCT
    CATTGTGTGT TTCCTCGGGA GTTGAAGGAA GTCTTTGCAT CTTGGAGGAT GCGCTGTGCA
    GAGCGCGGCC GGGAAGATAT AGCAGATCGA CTCATCAGTG CCTCTCTCTT CCTCCGATTC
    CTGTGTCCGG CCATTATGTC CCCCAGTCTC TTCAGCCTAA TGCAGGAATA CCCTGATGAG
    CAAACATCTA GGACCCTCAC GCTCATCGCA AAAGTCATCC AGAACCTGGC CAACTTCAAC
    TACAACAGGT TCAGCAGTAA AGAAGAATAT ATGTCCTTTA TGAATGAGTT CCTAGAGACG
    GAGTTGGCGA ATATGCAGAA TTTCCTGTAT GAGATCTCAA ACCTGGATTC GCTCACCAAC
    AGCATTAACT TTGAGGGCTA CATTGACCTT GGGCGGGAGC TGAGTACACT TCATGCCCTG
    CTTTGGGAAG TGATCCCACA ACTTAGTAAG GATGCACTTA TAAAGTTGGG GCCTCTTCCA
    CGCCTCCTTA ATGACATCAG CCTAGCTCTC AGGAACCCCC ACATCCAACG ACAACCAAGC
    CACCAAGAAC GACTGCCACC CCCCAAACCT GTCATTCTGA GGGGACCTTC AGCTGAGCTG
    CAAAGCTACT TGGTGCGAGA CCTCAACAGC TCTATTGACA TGACTCGCCT ACCCTCACCT
    ACTAAGGATA AACCATCAAA AGATATGTTT TTTGTTTCAC GTCGCCCGCT AACTCGCTCC
    TCGCCAGCCT ATTGCACCAG CAGCTCAGAC ATCACAGAAC CAGACCAGAA GATGCTGAGC
    GTGAACAAGA GTGTGTCCAT GATGGACTTG CAGGACAGCC GAATGAATAG CGTAACCAAC
    TTGCAGGCCG TAGGTGATCT GCTGAACTCC AGCCAGGCCT CCATATCAGG GGGCCTACGT
    CCGGGGGGCC GGCTCTCCCA GGGCAGTGGA TCCAGCATTG CAGCGGGTCT GAGGCTCAGT
    CAGATGGGGG TCACCACTGA TGGGCTCTCA GCTCAACAGC TACGCATTCC CTTGTCCTTC
    CAGAACCCGC TCTTCCACAT GGCTCCCGAT GGGCCTGGTG GGCCCCCATC TCATCCTGCT
    CCTCATCGAC CAGACCCCGG AGCTGATGCC TTTGCCCCTT TCCATGGGTA TAGCAAGAGT
    GAGGACTTGA GCTCCGGAAA GCATATGCTT CACAGTCACA GCTACAGCGA CGAATTCAGC
    CGGCAGGGTA AAAGGCAGTT ATCCCTCCAG GACAATCTCC AGCACATGCT TTCACCCCCT
    CAAATAACCA TTGGCCCTGG AGTGCCACGA AGGCAAGACT CACAGCCTCC CCCTTTGCAG
    AGGGGAAAAT CCCAACAACT GACGGTGCAG GCAGCGCATA AACCTCGGCC AAGCAGTGGC
    AACCTCCTAC AGAGCCCAGA ACCAATGTAT GGGGCAGCAA GAATTAGGCA GCAGTCAGTT
    GAAAAGGATG CAGGTGGCTT GAAACCATCA ATAACCAAGC AGCACTCCCA TGCACAGGCC
    ACTCTGAACC CTGTCACTCC AGCCTCAGAA CGCACAGTTG CCTGGGTATC CAACATGCCC
    CACCTTTCAG CAGATATTGA GAGCGCTCAC ATAGAACGGG AGGAGTATAA GTTGAAGGAA
    TACTCTAAAT CAATGGATGA GAGCCGTCTT GAGAGGGTGA AAGAGTATGA GGAAGAGATT
    TACTCCTTAA AAGAGAGGCT GTTGATGTCA AACCGGAAGC TGGAGGAATA TGAGAGGCGT
    CTTCTTACAC AGGAAGAACA AACTAATAAG ATGATGCAGC AATATCAGTC ACGCCTGGAC
    CAGAGTGAAA AGCGTTTGCG ACAGCAGCAG GTTGAGAAGG ACACGCAGAT AAAGAGCATC
    TTAAACAGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa46380
    Clone ID Related Accession (Same CDS sequence) XM_018096737.1
    Accession Version XM_018096737.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3648bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ras/Rap GTPase-activating protein SynGAP isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGATGTTCT ATGCTCCCTT CAGAGATGCT CGAGGACCTC CCATGCATCG TATGCCCTAT 
    GTCACCTCTC CATACGACCG TCCAGCCTGG AACCCAAGAT ACTGTGTCCT CTCTGGAAAT
    CAGCTTCTCA TGCTTGATGA GGAGGAGGTT CACCCGCTGT TGATACGTGA AAGGAGAACT
    GATCCAACCA GAACAAAGTT CCTAAGGCGC ACAGTCAGTG TCCCCGTGGA GGGGAAGCAG
    CCTGACGAAC ACGATTATCA CCTTGCACGT TCCCGGAGGA AAAGCGTCCC AGGGGGAAAA
    CAGCCCAGTA TGGACGCCCC TCCCACCGCC CCCTTTCGAC CGTCGCAAGG ATTCCTGAGC
    CGTCGCTTGA AGAGCTCTAT AAAGAGGACA AAGAGTCAGC CCAAATTAGA TAGGACCAGC
    AGTTTCCGGC AAATATTGCC AAGGTTTCGA AGTGCTGATC ATGATAGGGC ACGGCTCATG
    CAGGCCTTTA AAGAGTCACA CTCTCACGAG TCCTTGCTTA GTCCTAGCAG TGCTGCAGAG
    GCTTTGGATT TGAACTTGGA TGAGGATTCT GTGGTGAAGC CTGTACACAG CAGCATCTTG
    GGACAGGAGT TTTGCTTCGA GGTGACCACA TCATCAGGTA CCAAATGTTT TGCCTGCCGT
    TCTGCTGCTG AAAGAGACAA ATGGATTGAG AATCTTCAAA GAGCTGTAAA GCCCAATAAG
    GATAACAGCA GGCGGGTAGA TAATGTCTTA AAGCTGTGGA TTATTGAAGC CCGTGATCTT
    CCCCCCAAGA AGCGCTATTA TTGTGAGCTC TGTTTGGATG ACATTTTGTA TGCCCGTACC
    ACCAGCAAGC CTCGGAATGA CACTGTCTTT TGGGGGGAAC ACTTTGAATT TAATAACCTT
    CCAGCTGTTC GGAACTTGCG CTTGCACCTC TATAAGGACA CAGACAAGAA AAGGCGCAAA
    GAAAAGAGCA ACTATGTGGG CATGGTGAAC GTGCCTATTG CCAGCATTAG TGGGCGCCAC
    TTTGTGGAGC AGTGGTACCC GGTTTCTCAG CCCACATCCT ACAAGGGGAA GAGCAGCTGT
    CCCTCCATTC GCCTAAAATC CCGATACCAA ACCATGAGCA TCCTCCCCAT GGAGCTGTAC
    AAGGAGTTTG CAGAGTATGT CACCAACAAC TACCGGATGC TGTGCGCTGT ACTGGAGCCT
    GTGCTTAGCG TGAAGAGCAA AGAGGAGGTT GCCTGTGCTT TAGTGCATAT ACTGCAAAGC
    ACAGGCAAGG CCAAGGACTT CCTTTCAGAC ATGGCTATGA CCGAGGTTGA TCGCTTCCTT
    GACAGAGAGC ACCTTATTCT CCGTGAGAAC ACACTTGCAA CTAAAGCCAT AGAGGAGTAT
    CTGAAACTGA TAGGACAGAG ATACCTCAAG GAAGCCATTG GTGAGTTTAT TCGGGCACTG
    TATGAGTCTG AGGAGGACTG TGAGGTTGAT CCCATGCGTT GTCCACCCTC TGCATTGCCC
    GATCACCAGG CCAATCTCAG GATGTGCTGT GAACTCGCCC TTTGCAAAGT CTTTAACTCT
    CATTGTGTGT TTCCTCGGGA GTTGAAGGAA GTCTTTGCAT CTTGGAGGAT GCGCTGTGCA
    GAGCGCGGCC GGGAAGATAT AGCAGATCGA CTCATCAGTG CCTCTCTCTT CCTCCGATTC
    CTGTGTCCGG CCATTATGTC CCCCAGTCTC TTCAGCCTAA TGCAGGAATA CCCTGATGAG
    CAAACATCTA GGACCCTCAC GCTCATCGCA AAAGTCATCC AGAACCTGGC CAACTTCAAC
    TACAAGTTCA GCAGTAAAGA AGAATATATG TCCTTTATGA ATGAGTTCCT AGAGACGGAG
    TTGGCGAATA TGCAGAATTT CCTGTATGAG ATCTCAAACC TGGATTCGCT CACCAACAGC
    ATTAACTTTG AGGGCTACAT TGACCTTGGG CGGGAGCTGA GTACACTTCA TGCCCTGCTT
    TGGGAAGTGA TCCCACAACT TAGTAAGGAT GCACTTATAA AGTTGGGGCC TCTTCCACGC
    CTCCTTAATG ACATCAGCCT AGCTCTCAGG AACCCCCACA TCCAACGACA ACCAAGCCAC
    CAAGAACGAC TGCCACCCCC CAAACCTGTC ATTCTGAGGG GACCTTCAGC TGAGCTGCAA
    AGCTACTTGG TGCGAGACCT CAACAGCTCT ATTGACATGA CTCGCCTACC CTCACCTACT
    AAGGATAAAC CATCAAAAGA TATGTTTTTT GTTTCACGTC GCCCGCTAAC TCGCTCCTCG
    CCAGCCTATT GCACCAGCAG CTCAGACATC ACAGAACCAG ACCAGAAGAT GCTGAGCGTG
    AACAAGAGTG TGTCCATGAT GGACTTGCAG GACAGCCGAA TGAATAGCGT AACCAACTTG
    CAGGCCGTAG GTGATCTGCT GAACTCCAGC CAGGCCTCCA TATCAGGGGG CCTACGTCCG
    GGGGGCCGGC TCTCCCAGGG CAGTGGATCC AGCATTGCAG CGGGTCTGAG GCTCAGTCAG
    ATGGGGGTCA CCACTGATGG GCTCTCAGCT CAACAGCTAC GCATTCCCTT GTCCTTCCAG
    AACCCGCTCT TCCACATGGC TCCCGATGGG CCTGGTGGGC CCCCATCTCA TCCTGCTCCT
    CATCGACCAG ACCCCGGAGC TGATGCCTTT GCCCCTTTCC ATGGGTATAG CAAGAGTGAG
    GACTTGAGCT CCGGAAAGCA TATGCTTCAC AGTCACAGCT ACAGCGACGA ATTCAGCCGG
    CAGGGTAAAA GGCAGTTATC CCTCCAGGAC AATCTCCAGC ACATGCTTTC ACCCCCTCAA
    ATAACCATTG GCCCTGGAGT GCCACGAAGG CAAGACTCAC AGCCTCCCCC TTTGCAGAGG
    GGAAAATCCC AACAACTGAC GGTGCAGGCA GCGCATAAAC CTCGGCCAAG CAGTGGCAAC
    CTCCTACAGA GCCCAGAACC AATGTATGGG GCAGCAAGAA TTAGGCAGCA GTCAGTTGAA
    AAGGATGCAG GTGGCTTGAA ACCATCAATA ACCAAGCAGC ACTCCCATGC ACAGGCCACT
    CTGAACCCTG TCACTCCAGC CTCAGAACGC ACAGTTGCCT GGGTATCCAA CATGCCCCAC
    CTTTCAGCAG ATATTGAGAG CGCTCACATA GAACGGGAGG AGTATAAGTT GAAGGAATAC
    TCTAAATCAA TGGATGAGAG CCGTCTTGAG AGGGTGAAAG AGTATGAGGA AGAGATTTAC
    TCCTTAAAAG AGAGGCTGTT GATGTCAAAC CGGAAGCTGG AGGAATATGA GAGGCGTCTT
    CTTACACAGG AAGAACAAAC TAATAAGATG ATGCAGCAAT ATCAGTCACG CCTGGACCAG
    AGTGAAAAGC GTTTGCGACA GCAGCAGGTT GAGAAGGACA CGCAGATAAA GAGCATCTTA
    AACAGGTTGA TGCTGGTGGA GGAGGAGATC AGGAAGGATC ACTCACAAGA ACCAATCGAT
    ATGAAGAGGA GACTTATCGA GGCTCAGGAG AAGAAGTTTC CCTCCGTGGG TACAGCAGAC
    CCGCGTCTGA GGCTGGCGAC TCCATGGAAT GGGGCCCTCC CGCCTCTGCC TCCTCGACTC
    CAAATCACAG AAAATGGAGA CTTTCTAAAC TCAAGTGACC ATCACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017952226.1
    CDS940..4587
    Translation

    Target ORF information:

    RefSeq Version XM_018096737.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018096737.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGATGTTCT ATGCTCCCTT CAGAGATGCT CGAGGACCTC CCATGCATCG TATGCCCTAT 
    GTCACCTCTC CATACGACCG TCCAGCCTGG AACCCAAGAT ACTGTGTCCT CTCTGGAAAT
    CAGCTTCTCA TGCTTGATGA GGAGGAGGTT CACCCGCTGT TGATACGTGA AAGGAGAACT
    GATCCAACCA GAACAAAGTT CCTAAGGCGC ACAGTCAGTG TCCCCGTGGA GGGGAAGCAG
    CCTGACGAAC ACGATTATCA CCTTGCACGT TCCCGGAGGA AAAGCGTCCC AGGGGGAAAA
    CAGCCCAGTA TGGACGCCCC TCCCACCGCC CCCTTTCGAC CGTCGCAAGG ATTCCTGAGC
    CGTCGCTTGA AGAGCTCTAT AAAGAGGACA AAGAGTCAGC CCAAATTAGA TAGGACCAGC
    AGTTTCCGGC AAATATTGCC AAGGTTTCGA AGTGCTGATC ATGATAGGGC ACGGCTCATG
    CAGGCCTTTA AAGAGTCACA CTCTCACGAG TCCTTGCTTA GTCCTAGCAG TGCTGCAGAG
    GCTTTGGATT TGAACTTGGA TGAGGATTCT GTGGTGAAGC CTGTACACAG CAGCATCTTG
    GGACAGGAGT TTTGCTTCGA GGTGACCACA TCATCAGGTA CCAAATGTTT TGCCTGCCGT
    TCTGCTGCTG AAAGAGACAA ATGGATTGAG AATCTTCAAA GAGCTGTAAA GCCCAATAAG
    GATAACAGCA GGCGGGTAGA TAATGTCTTA AAGCTGTGGA TTATTGAAGC CCGTGATCTT
    CCCCCCAAGA AGCGCTATTA TTGTGAGCTC TGTTTGGATG ACATTTTGTA TGCCCGTACC
    ACCAGCAAGC CTCGGAATGA CACTGTCTTT TGGGGGGAAC ACTTTGAATT TAATAACCTT
    CCAGCTGTTC GGAACTTGCG CTTGCACCTC TATAAGGACA CAGACAAGAA AAGGCGCAAA
    GAAAAGAGCA ACTATGTGGG CATGGTGAAC GTGCCTATTG CCAGCATTAG TGGGCGCCAC
    TTTGTGGAGC AGTGGTACCC GGTTTCTCAG CCCACATCCT ACAAGGGGAA GAGCAGCTGT
    CCCTCCATTC GCCTAAAATC CCGATACCAA ACCATGAGCA TCCTCCCCAT GGAGCTGTAC
    AAGGAGTTTG CAGAGTATGT CACCAACAAC TACCGGATGC TGTGCGCTGT ACTGGAGCCT
    GTGCTTAGCG TGAAGAGCAA AGAGGAGGTT GCCTGTGCTT TAGTGCATAT ACTGCAAAGC
    ACAGGCAAGG CCAAGGACTT CCTTTCAGAC ATGGCTATGA CCGAGGTTGA TCGCTTCCTT
    GACAGAGAGC ACCTTATTCT CCGTGAGAAC ACACTTGCAA CTAAAGCCAT AGAGGAGTAT
    CTGAAACTGA TAGGACAGAG ATACCTCAAG GAAGCCATTG GTGAGTTTAT TCGGGCACTG
    TATGAGTCTG AGGAGGACTG TGAGGTTGAT CCCATGCGTT GTCCACCCTC TGCATTGCCC
    GATCACCAGG CCAATCTCAG GATGTGCTGT GAACTCGCCC TTTGCAAAGT CTTTAACTCT
    CATTGTGTGT TTCCTCGGGA GTTGAAGGAA GTCTTTGCAT CTTGGAGGAT GCGCTGTGCA
    GAGCGCGGCC GGGAAGATAT AGCAGATCGA CTCATCAGTG CCTCTCTCTT CCTCCGATTC
    CTGTGTCCGG CCATTATGTC CCCCAGTCTC TTCAGCCTAA TGCAGGAATA CCCTGATGAG
    CAAACATCTA GGACCCTCAC GCTCATCGCA AAAGTCATCC AGAACCTGGC CAACTTCAAC
    TACAAGTTCA GCAGTAAAGA AGAATATATG TCCTTTATGA ATGAGTTCCT AGAGACGGAG
    TTGGCGAATA TGCAGAATTT CCTGTATGAG ATCTCAAACC TGGATTCGCT CACCAACAGC
    ATTAACTTTG AGGGCTACAT TGACCTTGGG CGGGAGCTGA GTACACTTCA TGCCCTGCTT
    TGGGAAGTGA TCCCACAACT TAGTAAGGAT GCACTTATAA AGTTGGGGCC TCTTCCACGC
    CTCCTTAATG ACATCAGCCT AGCTCTCAGG AACCCCCACA TCCAACGACA ACCAAGCCAC
    CAAGAACGAC TGCCACCCCC CAAACCTGTC ATTCTGAGGG GACCTTCAGC TGAGCTGCAA
    AGCTACTTGG TGCGAGACCT CAACAGCTCT ATTGACATGA CTCGCCTACC CTCACCTACT
    AAGGATAAAC CATCAAAAGA TATGTTTTTT GTTTCACGTC GCCCGCTAAC TCGCTCCTCG
    CCAGCCTATT GCACCAGCAG CTCAGACATC ACAGAACCAG ACCAGAAGAT GCTGAGCGTG
    AACAAGAGTG TGTCCATGAT GGACTTGCAG GACAGCCGAA TGAATAGCGT AACCAACTTG
    CAGGCCGTAG GTGATCTGCT GAACTCCAGC CAGGCCTCCA TATCAGGGGG CCTACGTCCG
    GGGGGCCGGC TCTCCCAGGG CAGTGGATCC AGCATTGCAG CGGGTCTGAG GCTCAGTCAG
    ATGGGGGTCA CCACTGATGG GCTCTCAGCT CAACAGCTAC GCATTCCCTT GTCCTTCCAG
    AACCCGCTCT TCCACATGGC TCCCGATGGG CCTGGTGGGC CCCCATCTCA TCCTGCTCCT
    CATCGACCAG ACCCCGGAGC TGATGCCTTT GCCCCTTTCC ATGGGTATAG CAAGAGTGAG
    GACTTGAGCT CCGGAAAGCA TATGCTTCAC AGTCACAGCT ACAGCGACGA ATTCAGCCGG
    CAGGGTAAAA GGCAGTTATC CCTCCAGGAC AATCTCCAGC ACATGCTTTC ACCCCCTCAA
    ATAACCATTG GCCCTGGAGT GCCACGAAGG CAAGACTCAC AGCCTCCCCC TTTGCAGAGG
    GGAAAATCCC AACAACTGAC GGTGCAGGCA GCGCATAAAC CTCGGCCAAG CAGTGGCAAC
    CTCCTACAGA GCCCAGAACC AATGTATGGG GCAGCAAGAA TTAGGCAGCA GTCAGTTGAA
    AAGGATGCAG GTGGCTTGAA ACCATCAATA ACCAAGCAGC ACTCCCATGC ACAGGCCACT
    CTGAACCCTG TCACTCCAGC CTCAGAACGC ACAGTTGCCT GGGTATCCAA CATGCCCCAC
    CTTTCAGCAG ATATTGAGAG CGCTCACATA GAACGGGAGG AGTATAAGTT GAAGGAATAC
    TCTAAATCAA TGGATGAGAG CCGTCTTGAG AGGGTGAAAG AGTATGAGGA AGAGATTTAC
    TCCTTAAAAG AGAGGCTGTT GATGTCAAAC CGGAAGCTGG AGGAATATGA GAGGCGTCTT
    CTTACACAGG AAGAACAAAC TAATAAGATG ATGCAGCAAT ATCAGTCACG CCTGGACCAG
    AGTGAAAAGC GTTTGCGACA GCAGCAGGTT GAGAAGGACA CGCAGATAAA GAGCATCTTA
    AACAGGTTGA TGCTGGTGGA GGAGGAGATC AGGAAGGATC ACTCACAAGA ACCAATCGAT
    ATGAAGAGGA GACTTATCGA GGCTCAGGAG AAGAAGTTTC CCTCCGTGGG TACAGCAGAC
    CCGCGTCTGA GGCTGGCGAC TCCATGGAAT GGGGCCCTCC CGCCTCTGCC TCCTCGACTC
    CAAATCACAG AAAATGGAGA CTTTCTAAAC TCAAGTGACC ATCACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa65724
    Clone ID Related Accession (Same CDS sequence) XM_031892369.1
    Accession Version XM_031892369.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3669bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ras/Rap GTPase-activating protein SynGAP isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGATGTTCT ATGCTCCCTT CAGAGATGCT CGAGGACCTC CCATGCATCG TATGCCCTAT 
    GTCACCTCTC CATACGACCG TCCAGCCTGG AACCCAAGAT ACTGTGTCCT CTCTGGAAAT
    CAGCTTCTCA TGCTTGATGA GGAGGAGGTT CACCCGCTGT TGATACGTGA AAGGAGAACT
    GATCCAACCA GAACAAAGTT CCTAAGGCGC ACAGTCAGTG TCCCCGTGGA GGGGAAGCAG
    CCTGACGAAC ACGATTATCA CCTTGCACGT TCCCGGAGGA AAAGCGTCCC AGGGGGAAAA
    CAGCCCAGTA TGGACGCCCC TCCCACCGCC CCCTTTCGAC CGTCGCAAGG ATTCCTGAGC
    CGTCGCTTGA AGAGCTCTAT AAAGAGGACA AAGAGTCAGC CCAAATTAGA TAGGACCAGC
    AGTTTCCGGC AAATATTGCC AAGGTTTCGA AGTGCTGATC ATGATAGGGC ACGGCTCATG
    CAGGCCTTTA AAGAGTCACA CTCTCACGAG TCCTTGCTTA GTCCTAGCAG TGCTGCAGAG
    GCTTTGGATT TGAACTTGGA TGAGGATTCT GTGGTGAAGC CTGTACACAG CAGCATCTTG
    GGACAGGAGT TTTGCTTCGA GGTGACCACA TCATCAGGTA CCAAATGTTT TGCCTGCCGT
    TCTGCTGCTG AAAGAGACAA ATGGATTGAG AATCTTCAAA GAGCTGTAAA GCCCAATAAG
    GATAACAGCA GGCGGGTAGA TAATGTCTTA AAGCTGTGGA TTATTGAAGC CCGTGATCTT
    CCCCCCAAGA AGCGCTATTA TTGTGAGCTC TGTTTGGATG ACATTTTGTA TGCCCGTACC
    ACCAGCAAGC CTCGGAATGA CACTGTCTTT TGGGGGGAAC ACTTTGAATT TAATAACCTT
    CCAGCTGTTC GGAACTTGCG CTTGCACCTC TATAAGGACA CAGACAAGAA AAGGCGCAAA
    GAAAAGAGCA ACTATGTGGG CATGGTGAAC GTGCCTATTG CCAGCATTAG TGGGCGCCAC
    TTTGTGGAGC AGTGGTACCC GGTTTCTCAG CCCACATCCT ACAAGGGGAA GAGCAGCTGT
    CCCTCCATTC GCCTAAAATC CCGATACCAA ACCATGAGCA TCCTCCCCAT GGAGCTGTAC
    AAGGAGTTTG CAGAGTATGT CACCAACAAC TACCGGATGC TGTGCGCTGT ACTGGAGCCT
    GTGCTTAGCG TGAAGAGCAA AGAGGAGGTT GCCTGTGCTT TAGTGCATAT ACTGCAAAGC
    ACAGGCAAGG CCAAGGACTT CCTTTCAGAC ATGGCTATGA CCGAGGTTGA TCGCTTCCTT
    GACAGAGAGC ACCTTATTCT CCGTGAGAAC ACACTTGCAA CTAAAGCCAT AGAGGAGTAT
    CTGAAACTGA TAGGACAGAG ATACCTCAAG GAAGCCATTG GTGAGTTTAT TCGGGCACTG
    TATGAGTCTG AGGAGGACTG TGAGGTTGAT CCCATGCGTT GTCCACCCTC TGCATTGCCC
    GATCACCAGG CCAATCTCAG GATGTGCTGT GAACTCGCCC TTTGCAAAGT CTTTAACTCT
    CATTGTGTGT TTCCTCGGGA GTTGAAGGAA GTCTTTGCAT CTTGGAGGAT GCGCTGTGCA
    GAGCGCGGCC GGGAAGATAT AGCAGATCGA CTCATCAGTG CCTCTCTCTT CCTCCGATTC
    CTGTGTCCGG CCATTATGTC CCCCAGTCTC TTCAGCCTAA TGCAGGAATA CCCTGATGAG
    CAAACATCTA GGACCCTCAC GCTCATCGCA AAAGTCATCC AGAACCTGGC CAACTTCAAC
    TACAAGTTCA GCAGTAAAGA AGAATATATG TCCTTTATGA ATGAGTTCCT AGAGACGGAG
    TTGGCGAATA TGCAGAATTT CCTGTATGAG ATCTCAAACC TGGATTCGCT CACCAACAGC
    ATTAACTTTG AGGGCTACAT TGACCTTGGG CGGGAGCTGA GTACACTTCA TGCCCTGCTT
    TGGGAAGTGA TCCCACAACT TAGTAAGGAT GCACTTATAA AGTTGGGGCC TCTTCCACGC
    CTCCTTAATG ACATCAGCCT AGCTCTCAGG AACCCCCACA TCCAACGACA ACCAAGCCAC
    CAAGAACGAC TGCCACCCCC CAAACCTGTC ATTCTGAGGG GACCTTCAGC TGAGCTGCAA
    AGCTACTTGG TGCGAGACCT CAACAGCTCT ATTGACATGA CTCGCCTACC CTCACCTACT
    AAGGATAAAC CATCAAAAGA TATGTTTTTT GTTTCACGTC GCCCGCTAAC TCGCTCCTCG
    CCAGCCTATT GCACCAGCAG CTCAGACATC ACAGAACCAG ACCAGAAGAT GCTGAGCGTG
    AACAAGAGTG TGTCCATGAT GGACTTGCAG GACAGCCGAA TGAATAGCGT AACCAACTTG
    CAGGCCGTAG GTGATCTGCT GAACTCCAGC CAGGCCTCCA TATCAGGGGG CCTACGTCCG
    GGGGGCCGGC TCTCCCAGGG CAGTGGATCC AGCATTGCAG CGGGTCTGAG GCTCAGTCAG
    ATGGGGGTCA CCACTGATGG GCTCTCAGCT CAACAGCTAC GCATTCCCTT GTCCTTCCAG
    AACCCGCTCT TCCACATGGC TCCCGATGGG CCTGGTGGGC CCCCATCTCA TCCTGCTCCT
    CATCGACCAG ACCCCGGAGC TGATGCCTTT GCCCCTTTCC ATGGGTATAG CAAGAGTGAG
    GACTTGAGCT CCGGAAAGCA TATGCTTCAC AGTCACAGCT ACAGCGACGA ATTCAGCCGG
    CAGGGGTCAG AGTTCAGTAA AAGGCAGTTA TCCCTCCAGG ACAATCTCCA GCACATGCTT
    TCACCCCCTC AAATAACCAT TGGCCCTGGA GTGCCACGAA GGCAAGACTC ACAGCCTCCC
    CCTTTGCAGA GGGGAAAATC CCAACAACTG ACGGTGCAGG CAGCGCATAA ACCTCGGCCA
    AGCAGTGGCA ACCTCCTACA GAGCCCAGAA CCAATGTATG GGGCAGCAAG AATTAGGCAG
    CAGTCAGTTG AAAAGGATGC AGGTGGCTTG AAACCATCAA TAACCAAGCA GCACTCCCAT
    GCACAGGCCA CTCTGAACCC TGTCACTCCA GCCTCAGAAC GCACAGTTGC CTGGGTATCC
    AACATGCCCC ACCTTTCAGC AGATATTGAG AGCGCTCACA TAGAACGGGA GGAGTATAAG
    TTGAAGGAAT ACTCTAAATC AATGGATGAG AGCCGTCTTG AGAGGGTGAA AGAGTATGAG
    GAAGAGATTT ACTCCTTAAA AGAGAGGCTG TTGATGTCAA ACCGGAAGCT GGAGGAATAT
    GAGAGGCGTC TTCTTACACA GGAAGAACAA ACTAATAAGA TGATGCAGCA ATATCAGTCA
    CGCCTGGACC AGAGTGAAAA GCGTTTGCGA CAGCAGCAGG TTGAGAAGGA CACGCAGATA
    AAGAGCATCT TAAACAGGTT AGAAATGGTT GATGCTGGTG GAGGAGGAGA TCAGGAAGGA
    TCACTCACAA GAACCAATCG ATATGAAGAG GAGACTTATC GAGGCTCAGA GAAGAAGTTT
    CCCTCCGTGG GTACAGCAGA CCCGCGTCTG AGGCTGGCGA CTCCATGGAA TGGGGCCCTC
    CCGCCTCTGC CTCCTCGACT CCAAATCACA GAAAATGGAG ACTTTCTAAA CTCAAGTGAC
    CATCACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031748229.1
    CDS665..4333
    Translation

    Target ORF information:

    RefSeq Version XM_031892369.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031892369.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGATGTTCT ATGCTCCCTT CAGAGATGCT CGAGGACCTC CCATGCATCG TATGCCCTAT 
    GTCACCTCTC CATACGACCG TCCAGCCTGG AACCCAAGAT ACTGTGTCCT CTCTGGAAAT
    CAGCTTCTCA TGCTTGATGA GGAGGAGGTT CACCCGCTGT TGATACGTGA AAGGAGAACT
    GATCCAACCA GAACAAAGTT CCTAAGGCGC ACAGTCAGTG TCCCCGTGGA GGGGAAGCAG
    CCTGACGAAC ACGATTATCA CCTTGCACGT TCCCGGAGGA AAAGCGTCCC AGGGGGAAAA
    CAGCCCAGTA TGGACGCCCC TCCCACCGCC CCCTTTCGAC CGTCGCAAGG ATTCCTGAGC
    CGTCGCTTGA AGAGCTCTAT AAAGAGGACA AAGAGTCAGC CCAAATTAGA TAGGACCAGC
    AGTTTCCGGC AAATATTGCC AAGGTTTCGA AGTGCTGATC ATGATAGGGC ACGGCTCATG
    CAGGCCTTTA AAGAGTCACA CTCTCACGAG TCCTTGCTTA GTCCTAGCAG TGCTGCAGAG
    GCTTTGGATT TGAACTTGGA TGAGGATTCT GTGGTGAAGC CTGTACACAG CAGCATCTTG
    GGACAGGAGT TTTGCTTCGA GGTGACCACA TCATCAGGTA CCAAATGTTT TGCCTGCCGT
    TCTGCTGCTG AAAGAGACAA ATGGATTGAG AATCTTCAAA GAGCTGTAAA GCCCAATAAG
    GATAACAGCA GGCGGGTAGA TAATGTCTTA AAGCTGTGGA TTATTGAAGC CCGTGATCTT
    CCCCCCAAGA AGCGCTATTA TTGTGAGCTC TGTTTGGATG ACATTTTGTA TGCCCGTACC
    ACCAGCAAGC CTCGGAATGA CACTGTCTTT TGGGGGGAAC ACTTTGAATT TAATAACCTT
    CCAGCTGTTC GGAACTTGCG CTTGCACCTC TATAAGGACA CAGACAAGAA AAGGCGCAAA
    GAAAAGAGCA ACTATGTGGG CATGGTGAAC GTGCCTATTG CCAGCATTAG TGGGCGCCAC
    TTTGTGGAGC AGTGGTACCC GGTTTCTCAG CCCACATCCT ACAAGGGGAA GAGCAGCTGT
    CCCTCCATTC GCCTAAAATC CCGATACCAA ACCATGAGCA TCCTCCCCAT GGAGCTGTAC
    AAGGAGTTTG CAGAGTATGT CACCAACAAC TACCGGATGC TGTGCGCTGT ACTGGAGCCT
    GTGCTTAGCG TGAAGAGCAA AGAGGAGGTT GCCTGTGCTT TAGTGCATAT ACTGCAAAGC
    ACAGGCAAGG CCAAGGACTT CCTTTCAGAC ATGGCTATGA CCGAGGTTGA TCGCTTCCTT
    GACAGAGAGC ACCTTATTCT CCGTGAGAAC ACACTTGCAA CTAAAGCCAT AGAGGAGTAT
    CTGAAACTGA TAGGACAGAG ATACCTCAAG GAAGCCATTG GTGAGTTTAT TCGGGCACTG
    TATGAGTCTG AGGAGGACTG TGAGGTTGAT CCCATGCGTT GTCCACCCTC TGCATTGCCC
    GATCACCAGG CCAATCTCAG GATGTGCTGT GAACTCGCCC TTTGCAAAGT CTTTAACTCT
    CATTGTGTGT TTCCTCGGGA GTTGAAGGAA GTCTTTGCAT CTTGGAGGAT GCGCTGTGCA
    GAGCGCGGCC GGGAAGATAT AGCAGATCGA CTCATCAGTG CCTCTCTCTT CCTCCGATTC
    CTGTGTCCGG CCATTATGTC CCCCAGTCTC TTCAGCCTAA TGCAGGAATA CCCTGATGAG
    CAAACATCTA GGACCCTCAC GCTCATCGCA AAAGTCATCC AGAACCTGGC CAACTTCAAC
    TACAAGTTCA GCAGTAAAGA AGAATATATG TCCTTTATGA ATGAGTTCCT AGAGACGGAG
    TTGGCGAATA TGCAGAATTT CCTGTATGAG ATCTCAAACC TGGATTCGCT CACCAACAGC
    ATTAACTTTG AGGGCTACAT TGACCTTGGG CGGGAGCTGA GTACACTTCA TGCCCTGCTT
    TGGGAAGTGA TCCCACAACT TAGTAAGGAT GCACTTATAA AGTTGGGGCC TCTTCCACGC
    CTCCTTAATG ACATCAGCCT AGCTCTCAGG AACCCCCACA TCCAACGACA ACCAAGCCAC
    CAAGAACGAC TGCCACCCCC CAAACCTGTC ATTCTGAGGG GACCTTCAGC TGAGCTGCAA
    AGCTACTTGG TGCGAGACCT CAACAGCTCT ATTGACATGA CTCGCCTACC CTCACCTACT
    AAGGATAAAC CATCAAAAGA TATGTTTTTT GTTTCACGTC GCCCGCTAAC TCGCTCCTCG
    CCAGCCTATT GCACCAGCAG CTCAGACATC ACAGAACCAG ACCAGAAGAT GCTGAGCGTG
    AACAAGAGTG TGTCCATGAT GGACTTGCAG GACAGCCGAA TGAATAGCGT AACCAACTTG
    CAGGCCGTAG GTGATCTGCT GAACTCCAGC CAGGCCTCCA TATCAGGGGG CCTACGTCCG
    GGGGGCCGGC TCTCCCAGGG CAGTGGATCC AGCATTGCAG CGGGTCTGAG GCTCAGTCAG
    ATGGGGGTCA CCACTGATGG GCTCTCAGCT CAACAGCTAC GCATTCCCTT GTCCTTCCAG
    AACCCGCTCT TCCACATGGC TCCCGATGGG CCTGGTGGGC CCCCATCTCA TCCTGCTCCT
    CATCGACCAG ACCCCGGAGC TGATGCCTTT GCCCCTTTCC ATGGGTATAG CAAGAGTGAG
    GACTTGAGCT CCGGAAAGCA TATGCTTCAC AGTCACAGCT ACAGCGACGA ATTCAGCCGG
    CAGGGGTCAG AGTTCAGTAA AAGGCAGTTA TCCCTCCAGG ACAATCTCCA GCACATGCTT
    TCACCCCCTC AAATAACCAT TGGCCCTGGA GTGCCACGAA GGCAAGACTC ACAGCCTCCC
    CCTTTGCAGA GGGGAAAATC CCAACAACTG ACGGTGCAGG CAGCGCATAA ACCTCGGCCA
    AGCAGTGGCA ACCTCCTACA GAGCCCAGAA CCAATGTATG GGGCAGCAAG AATTAGGCAG
    CAGTCAGTTG AAAAGGATGC AGGTGGCTTG AAACCATCAA TAACCAAGCA GCACTCCCAT
    GCACAGGCCA CTCTGAACCC TGTCACTCCA GCCTCAGAAC GCACAGTTGC CTGGGTATCC
    AACATGCCCC ACCTTTCAGC AGATATTGAG AGCGCTCACA TAGAACGGGA GGAGTATAAG
    TTGAAGGAAT ACTCTAAATC AATGGATGAG AGCCGTCTTG AGAGGGTGAA AGAGTATGAG
    GAAGAGATTT ACTCCTTAAA AGAGAGGCTG TTGATGTCAA ACCGGAAGCT GGAGGAATAT
    GAGAGGCGTC TTCTTACACA GGAAGAACAA ACTAATAAGA TGATGCAGCA ATATCAGTCA
    CGCCTGGACC AGAGTGAAAA GCGTTTGCGA CAGCAGCAGG TTGAGAAGGA CACGCAGATA
    AAGAGCATCT TAAACAGGTT AGAAATGGTT GATGCTGGTG GAGGAGGAGA TCAGGAAGGA
    TCACTCACAA GAACCAATCG ATATGAAGAG GAGACTTATC GAGGCTCAGA GAAGAAGTTT
    CCCTCCGTGG GTACAGCAGA CCCGCGTCTG AGGCTGGCGA CTCCATGGAA TGGGGCCCTC
    CCGCCTCTGC CTCCTCGACT CCAAATCACA GAAAATGGAG ACTTTCTAAA CTCAAGTGAC
    CATCACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa65725
    Clone ID Related Accession (Same CDS sequence) XM_031892370.1
    Accession Version XM_031892370.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3663bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ras/Rap GTPase-activating protein SynGAP isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGATGTTCT ATGCTCCCTT CAGAGATGCT CGAGGACCTC CCATGCATCG TATGCCCTAT 
    GTCACCTCTC CATACGACCG TCCAGCCTGG AACCCAAGAT ACTGTGTCCT CTCTGGAAAT
    CAGCTTCTCA TGCTTGATGA GGAGGAGGTT CACCCGCTGT TGATACGTGA AAGGAGAACT
    GATCCAACCA GAACAAAGTT CCTAAGGCGC ACAGTCAGTG TCCCCGTGGA GGGGAAGCAG
    CCTGACGAAC ACGATTATCA CCTTGCACGT TCCCGGAGGA AAAGCGTCCC AGGGGGAAAA
    CAGCCCAGTA TGGACGCCCC TCCCACCGCC CCCTTTCGAC CGTCGCAAGG ATTCCTGAGC
    CGTCGCTTGA AGAGCTCTAT AAAGAGGACA AAGAGTCAGC CCAAATTAGA TAGGACCAGC
    AGTTTCCGGC AAATATTGCC AAGGTTTCGA AGTGCTGATC ATGATAGGGC ACGGCTCATG
    CAGGCCTTTA AAGAGTCACA CTCTCACGAG TCCTTGCTTA GTCCTAGCAG TGCTGCAGAG
    GCTTTGGATT TGAACTTGGA TGAGGATTCT GTGGTGAAGC CTGTACACAG CAGCATCTTG
    GGACAGGAGT TTTGCTTCGA GGTGACCACA TCATCAGGTA CCAAATGTTT TGCCTGCCGT
    TCTGCTGCTG AAAGAGACAA ATGGATTGAG AATCTTCAAA GAGCTGTAAA GCCCAATAAG
    GATAACAGCA GGCGGGTAGA TAATGTCTTA AAGCTGTGGA TTATTGAAGC CCGTGATCTT
    CCCCCCAAGA AGCGCTATTA TTGTGAGCTC TGTTTGGATG ACATTTTGTA TGCCCGTACC
    ACCAGCAAGC CTCGGAATGA CACTGTCTTT TGGGGGGAAC ACTTTGAATT TAATAACCTT
    CCAGCTGTTC GGAACTTGCG CTTGCACCTC TATAAGGACA CAGACAAGAA AAGGCGCAAA
    GAAAAGAGCA ACTATGTGGG CATGGTGAAC GTGCCTATTG CCAGCATTAG TGGGCGCCAC
    TTTGTGGAGC AGTGGTACCC GGTTTCTCAG CCCACATCCT ACAAGGGGAA GAGCAGCTGT
    CCCTCCATTC GCCTAAAATC CCGATACCAA ACCATGAGCA TCCTCCCCAT GGAGCTGTAC
    AAGGAGTTTG CAGAGTATGT CACCAACAAC TACCGGATGC TGTGCGCTGT ACTGGAGCCT
    GTGCTTAGCG TGAAGAGCAA AGAGGAGGTT GCCTGTGCTT TAGTGCATAT ACTGCAAAGC
    ACAGGCAAGG CCAAGGACTT CCTTTCAGAC ATGGCTATGA CCGAGGTTGA TCGCTTCCTT
    GACAGAGAGC ACCTTATTCT CCGTGAGAAC ACACTTGCAA CTAAAGCCAT AGAGGAGTAT
    CTGAAACTGA TAGGACAGAG ATACCTCAAG GAAGCCATTG GTGAGTTTAT TCGGGCACTG
    TATGAGTCTG AGGAGGACTG TGAGGTTGAT CCCATGCGTT GTCCACCCTC TGCATTGCCC
    GATCACCAGG CCAATCTCAG GATGTGCTGT GAACTCGCCC TTTGCAAAGT CTTTAACTCT
    CATTGTGTGT TTCCTCGGGA GTTGAAGGAA GTCTTTGCAT CTTGGAGGAT GCGCTGTGCA
    GAGCGCGGCC GGGAAGATAT AGCAGATCGA CTCATCAGTG CCTCTCTCTT CCTCCGATTC
    CTGTGTCCGG CCATTATGTC CCCCAGTCTC TTCAGCCTAA TGCAGGAATA CCCTGATGAG
    CAAACATCTA GGACCCTCAC GCTCATCGCA AAAGTCATCC AGAACCTGGC CAACTTCAAC
    TACAACAGGT TCAGCAGTAA AGAAGAATAT ATGTCCTTTA TGAATGAGTT CCTAGAGACG
    GAGTTGGCGA ATATGCAGAA TTTCCTGTAT GAGATCTCAA ACCTGGATTC GCTCACCAAC
    AGCATTAACT TTGAGGGCTA CATTGACCTT GGGCGGGAGC TGAGTACACT TCATGCCCTG
    CTTTGGGAAG TGATCCCACA ACTTAGTAAG GATGCACTTA TAAAGTTGGG GCCTCTTCCA
    CGCCTCCTTA ATGACATCAG CCTAGCTCTC AGGAACCCCC ACATCCAACG ACAACCAAGC
    CACCAAGAAC GACTGCCACC CCCCAAACCT GTCATTCTGA GGGGACCTTC AGCTGAGCTG
    CAAAGCTACT TGGTGCGAGA CCTCAACAGC TCTATTGACA TGACTCGCCT ACCCTCACCT
    ACTAAGGATA AACCATCAAA AGATATGTTT TTTGTTTCAC GTCGCCCGCT AACTCGCTCC
    TCGCCAGCCT ATTGCACCAG CAGCTCAGAC ATCACAGAAC CAGACCAGAA GATGCTGAGC
    GTGAACAAGA GTGTGTCCAT GATGGACTTG CAGGACAGCC GAATGAATAG CGTAACCAAC
    TTGCAGGCCG TAGGTGATCT GCTGAACTCC AGCCAGGCCT CCATATCAGG GGGCCTACGT
    CCGGGGGGCC GGCTCTCCCA GGGCAGTGGA TCCAGCATTG CAGCGGGTCT GAGGCTCAGT
    CAGATGGGGG TCACCACTGA TGGGCTCTCA GCTCAACAGC TACGCATTCC CTTGTCCTTC
    CAGAACCCGC TCTTCCACAT GGCTCCCGAT GGGCCTGGTG GGCCCCCATC TCATCCTGCT
    CCTCATCGAC CAGACCCCGG AGCTGATGCC TTTGCCCCTT TCCATGGGTA TAGCAAGAGT
    GAGGACTTGA GCTCCGGAAA GCATATGCTT CACAGTCACA GCTACAGCGA CGAATTCAGC
    CGGCAGGGGT CAGAGTTCAG TAAAAGGCAG TTATCCCTCC AGGACAATCT CCAGCACATG
    CTTTCACCCC CTCAAATAAC CATTGGCCCT GGAGTGCCAC GAAGGCAAGA CTCACAGCCT
    CCCCCTTTGC AGAGGGGAAA ATCCCAACAA CTGACGGTGC AGGCAGCGCA TAAACCTCGG
    CCAAGCAGTG GCAACCTCCT ACAGAGCCCA GAACCAATGT ATGGGGCAGC AAGAATTAGG
    CAGCAGTCAG TTGAAAAGGA TGCAGGTGGC TTGAAACCAT CAATAACCAA GCAGCACTCC
    CATGCACAGG CCACTCTGAA CCCTGTCACT CCAGCCTCAG AACGCACAGT TGCCTGGGTA
    TCCAACATGC CCCACCTTTC AGCAGATATT GAGAGCGCTC ACATAGAACG GGAGGAGTAT
    AAGTTGAAGG AATACTCTAA ATCAATGGAT GAGAGCCGTC TTGAGAGGGT GAAAGAGTAT
    GAGGAAGAGA TTTACTCCTT AAAAGAGAGG CTGTTGATGT CAAACCGGAA GCTGGAGGAA
    TATGAGAGGC GTCTTCTTAC ACAGGAAGAA CAAACTAATA AGATGATGCA GCAATATCAG
    TCACGCCTGG ACCAGAGTGA AAAGCGTTTG CGACAGCAGC AGGTTGAGAA GGACACGCAG
    ATAAAGAGCA TCTTAAACAG GTTAGAAATG GTTGATGCTG GTGGAGGAGG AGATCAGGAA
    GGATCACTCA CAAGAACCAA TCGATATGAA GAGGAGACTT ATCGAGGCTC AGGAGAAGAA
    GTTTCCCTCC GTGGGTACAG CAGACCCGCG TCTGAGGCTG GCGACTCCAT GGAATGGGGC
    CCTCCCGCCT CTGCCTCCTC GACTCCAAAT CACAGAAAAT GGAGACTTTC TAAACTCAAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031748230.1
    CDS665..4327
    Translation

    Target ORF information:

    RefSeq Version XM_031892370.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031892370.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGATGTTCT ATGCTCCCTT CAGAGATGCT CGAGGACCTC CCATGCATCG TATGCCCTAT 
    GTCACCTCTC CATACGACCG TCCAGCCTGG AACCCAAGAT ACTGTGTCCT CTCTGGAAAT
    CAGCTTCTCA TGCTTGATGA GGAGGAGGTT CACCCGCTGT TGATACGTGA AAGGAGAACT
    GATCCAACCA GAACAAAGTT CCTAAGGCGC ACAGTCAGTG TCCCCGTGGA GGGGAAGCAG
    CCTGACGAAC ACGATTATCA CCTTGCACGT TCCCGGAGGA AAAGCGTCCC AGGGGGAAAA
    CAGCCCAGTA TGGACGCCCC TCCCACCGCC CCCTTTCGAC CGTCGCAAGG ATTCCTGAGC
    CGTCGCTTGA AGAGCTCTAT AAAGAGGACA AAGAGTCAGC CCAAATTAGA TAGGACCAGC
    AGTTTCCGGC AAATATTGCC AAGGTTTCGA AGTGCTGATC ATGATAGGGC ACGGCTCATG
    CAGGCCTTTA AAGAGTCACA CTCTCACGAG TCCTTGCTTA GTCCTAGCAG TGCTGCAGAG
    GCTTTGGATT TGAACTTGGA TGAGGATTCT GTGGTGAAGC CTGTACACAG CAGCATCTTG
    GGACAGGAGT TTTGCTTCGA GGTGACCACA TCATCAGGTA CCAAATGTTT TGCCTGCCGT
    TCTGCTGCTG AAAGAGACAA ATGGATTGAG AATCTTCAAA GAGCTGTAAA GCCCAATAAG
    GATAACAGCA GGCGGGTAGA TAATGTCTTA AAGCTGTGGA TTATTGAAGC CCGTGATCTT
    CCCCCCAAGA AGCGCTATTA TTGTGAGCTC TGTTTGGATG ACATTTTGTA TGCCCGTACC
    ACCAGCAAGC CTCGGAATGA CACTGTCTTT TGGGGGGAAC ACTTTGAATT TAATAACCTT
    CCAGCTGTTC GGAACTTGCG CTTGCACCTC TATAAGGACA CAGACAAGAA AAGGCGCAAA
    GAAAAGAGCA ACTATGTGGG CATGGTGAAC GTGCCTATTG CCAGCATTAG TGGGCGCCAC
    TTTGTGGAGC AGTGGTACCC GGTTTCTCAG CCCACATCCT ACAAGGGGAA GAGCAGCTGT
    CCCTCCATTC GCCTAAAATC CCGATACCAA ACCATGAGCA TCCTCCCCAT GGAGCTGTAC
    AAGGAGTTTG CAGAGTATGT CACCAACAAC TACCGGATGC TGTGCGCTGT ACTGGAGCCT
    GTGCTTAGCG TGAAGAGCAA AGAGGAGGTT GCCTGTGCTT TAGTGCATAT ACTGCAAAGC
    ACAGGCAAGG CCAAGGACTT CCTTTCAGAC ATGGCTATGA CCGAGGTTGA TCGCTTCCTT
    GACAGAGAGC ACCTTATTCT CCGTGAGAAC ACACTTGCAA CTAAAGCCAT AGAGGAGTAT
    CTGAAACTGA TAGGACAGAG ATACCTCAAG GAAGCCATTG GTGAGTTTAT TCGGGCACTG
    TATGAGTCTG AGGAGGACTG TGAGGTTGAT CCCATGCGTT GTCCACCCTC TGCATTGCCC
    GATCACCAGG CCAATCTCAG GATGTGCTGT GAACTCGCCC TTTGCAAAGT CTTTAACTCT
    CATTGTGTGT TTCCTCGGGA GTTGAAGGAA GTCTTTGCAT CTTGGAGGAT GCGCTGTGCA
    GAGCGCGGCC GGGAAGATAT AGCAGATCGA CTCATCAGTG CCTCTCTCTT CCTCCGATTC
    CTGTGTCCGG CCATTATGTC CCCCAGTCTC TTCAGCCTAA TGCAGGAATA CCCTGATGAG
    CAAACATCTA GGACCCTCAC GCTCATCGCA AAAGTCATCC AGAACCTGGC CAACTTCAAC
    TACAACAGGT TCAGCAGTAA AGAAGAATAT ATGTCCTTTA TGAATGAGTT CCTAGAGACG
    GAGTTGGCGA ATATGCAGAA TTTCCTGTAT GAGATCTCAA ACCTGGATTC GCTCACCAAC
    AGCATTAACT TTGAGGGCTA CATTGACCTT GGGCGGGAGC TGAGTACACT TCATGCCCTG
    CTTTGGGAAG TGATCCCACA ACTTAGTAAG GATGCACTTA TAAAGTTGGG GCCTCTTCCA
    CGCCTCCTTA ATGACATCAG CCTAGCTCTC AGGAACCCCC ACATCCAACG ACAACCAAGC
    CACCAAGAAC GACTGCCACC CCCCAAACCT GTCATTCTGA GGGGACCTTC AGCTGAGCTG
    CAAAGCTACT TGGTGCGAGA CCTCAACAGC TCTATTGACA TGACTCGCCT ACCCTCACCT
    ACTAAGGATA AACCATCAAA AGATATGTTT TTTGTTTCAC GTCGCCCGCT AACTCGCTCC
    TCGCCAGCCT ATTGCACCAG CAGCTCAGAC ATCACAGAAC CAGACCAGAA GATGCTGAGC
    GTGAACAAGA GTGTGTCCAT GATGGACTTG CAGGACAGCC GAATGAATAG CGTAACCAAC
    TTGCAGGCCG TAGGTGATCT GCTGAACTCC AGCCAGGCCT CCATATCAGG GGGCCTACGT
    CCGGGGGGCC GGCTCTCCCA GGGCAGTGGA TCCAGCATTG CAGCGGGTCT GAGGCTCAGT
    CAGATGGGGG TCACCACTGA TGGGCTCTCA GCTCAACAGC TACGCATTCC CTTGTCCTTC
    CAGAACCCGC TCTTCCACAT GGCTCCCGAT GGGCCTGGTG GGCCCCCATC TCATCCTGCT
    CCTCATCGAC CAGACCCCGG AGCTGATGCC TTTGCCCCTT TCCATGGGTA TAGCAAGAGT
    GAGGACTTGA GCTCCGGAAA GCATATGCTT CACAGTCACA GCTACAGCGA CGAATTCAGC
    CGGCAGGGGT CAGAGTTCAG TAAAAGGCAG TTATCCCTCC AGGACAATCT CCAGCACATG
    CTTTCACCCC CTCAAATAAC CATTGGCCCT GGAGTGCCAC GAAGGCAAGA CTCACAGCCT
    CCCCCTTTGC AGAGGGGAAA ATCCCAACAA CTGACGGTGC AGGCAGCGCA TAAACCTCGG
    CCAAGCAGTG GCAACCTCCT ACAGAGCCCA GAACCAATGT ATGGGGCAGC AAGAATTAGG
    CAGCAGTCAG TTGAAAAGGA TGCAGGTGGC TTGAAACCAT CAATAACCAA GCAGCACTCC
    CATGCACAGG CCACTCTGAA CCCTGTCACT CCAGCCTCAG AACGCACAGT TGCCTGGGTA
    TCCAACATGC CCCACCTTTC AGCAGATATT GAGAGCGCTC ACATAGAACG GGAGGAGTAT
    AAGTTGAAGG AATACTCTAA ATCAATGGAT GAGAGCCGTC TTGAGAGGGT GAAAGAGTAT
    GAGGAAGAGA TTTACTCCTT AAAAGAGAGG CTGTTGATGT CAAACCGGAA GCTGGAGGAA
    TATGAGAGGC GTCTTCTTAC ACAGGAAGAA CAAACTAATA AGATGATGCA GCAATATCAG
    TCACGCCTGG ACCAGAGTGA AAAGCGTTTG CGACAGCAGC AGGTTGAGAA GGACACGCAG
    ATAAAGAGCA TCTTAAACAG GTTAGAAATG GTTGATGCTG GTGGAGGAGG AGATCAGGAA
    GGATCACTCA CAAGAACCAA TCGATATGAA GAGGAGACTT ATCGAGGCTC AGGAGAAGAA
    GTTTCCCTCC GTGGGTACAG CAGACCCGCG TCTGAGGCTG GCGACTCCAT GGAATGGGGC
    CCTCCCGCCT CTGCCTCCTC GACTCCAAAT CACAGAAAAT GGAGACTTTC TAAACTCAAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa65726
    Clone ID Related Accession (Same CDS sequence) XM_031892371.1
    Accession Version XM_031892371.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3663bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ras/Rap GTPase-activating protein SynGAP isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGATGTTCT ATGCTCCCTT CAGAGATGCT CGAGGACCTC CCATGCATCG TATGCCCTAT 
    GTCACCTCTC CATACGACCG TCCAGCCTGG AACCCAAGAT ACTGTGTCCT CTCTGGAAAT
    CAGCTTCTCA TGCTTGATGA GGAGGAGGTT CACCCGCTGT TGATACGTGA AAGGAGAACT
    GATCCAACCA GAACAAAGTT CCTAAGGCGC ACAGTCAGTG TCCCCGTGGA GGGGAAGCAG
    CCTGACGAAC ACGATTATCA CCTTGCACGT TCCCGGAGGA AAAGCGTCCC AGGGGGAAAA
    CAGCCCAGTA TGGACGCCCC TCCCACCGCC CCCTTTCGAC CGTCGCAAGG ATTCCTGAGC
    CGTCGCTTGA AGAGCTCTAT AAAGAGGACA AAGAGTCAGC CCAAATTAGA TAGGACCAGC
    AGTTTCCGGC AAATATTGCC AAGGTTTCGA AGTGCTGATC ATGATAGGGC ACGGCTCATG
    CAGGCCTTTA AAGAGTCACA CTCTCACGAG TCCTTGCTTA GTCCTAGCAG TGCTGCAGAG
    GCTTTGGATT TGAACTTGGA TGAGGATTCT GTGGTGAAGC CTGTACACAG CAGCATCTTG
    GGACAGGAGT TTTGCTTCGA GGTGACCACA TCATCAGGTA CCAAATGTTT TGCCTGCCGT
    TCTGCTGCTG AAAGAGACAA ATGGATTGAG AATCTTCAAA GAGCTGTAAA GCCCAATAAG
    GATAACAGCA GGCGGGTAGA TAATGTCTTA AAGCTGTGGA TTATTGAAGC CCGTGATCTT
    CCCCCCAAGA AGCGCTATTA TTGTGAGCTC TGTTTGGATG ACATTTTGTA TGCCCGTACC
    ACCAGCAAGC CTCGGAATGA CACTGTCTTT TGGGGGGAAC ACTTTGAATT TAATAACCTT
    CCAGCTGTTC GGAACTTGCG CTTGCACCTC TATAAGGACA CAGACAAGAA AAGGCGCAAA
    GAAAAGAGCA ACTATGTGGG CATGGTGAAC GTGCCTATTG CCAGCATTAG TGGGCGCCAC
    TTTGTGGAGC AGTGGTACCC GGTTTCTCAG CCCACATCCT ACAAGGGGAA GAGCAGCTGT
    CCCTCCATTC GCCTAAAATC CCGATACCAA ACCATGAGCA TCCTCCCCAT GGAGCTGTAC
    AAGGAGTTTG CAGAGTATGT CACCAACAAC TACCGGATGC TGTGCGCTGT ACTGGAGCCT
    GTGCTTAGCG TGAAGAGCAA AGAGGAGGTT GCCTGTGCTT TAGTGCATAT ACTGCAAAGC
    ACAGGCAAGG CCAAGGACTT CCTTTCAGAC ATGGCTATGA CCGAGGTTGA TCGCTTCCTT
    GACAGAGAGC ACCTTATTCT CCGTGAGAAC ACACTTGCAA CTAAAGCCAT AGAGGAGTAT
    CTGAAACTGA TAGGACAGAG ATACCTCAAG GAAGCCATTG GTGAGTTTAT TCGGGCACTG
    TATGAGTCTG AGGAGGACTG TGAGGTTGAT CCCATGCGTT GTCCACCCTC TGCATTGCCC
    GATCACCAGG CCAATCTCAG GATGTGCTGT GAACTCGCCC TTTGCAAAGT CTTTAACTCT
    CATTGTGTGT TTCCTCGGGA GTTGAAGGAA GTCTTTGCAT CTTGGAGGAT GCGCTGTGCA
    GAGCGCGGCC GGGAAGATAT AGCAGATCGA CTCATCAGTG CCTCTCTCTT CCTCCGATTC
    CTGTGTCCGG CCATTATGTC CCCCAGTCTC TTCAGCCTAA TGCAGGAATA CCCTGATGAG
    CAAACATCTA GGACCCTCAC GCTCATCGCA AAAGTCATCC AGAACCTGGC CAACTTCAAC
    TACAACAGGT TCAGCAGTAA AGAAGAATAT ATGTCCTTTA TGAATGAGTT CCTAGAGACG
    GAGTTGGCGA ATATGCAGAA TTTCCTGTAT GAGATCTCAA ACCTGGATTC GCTCACCAAC
    AGCATTAACT TTGAGGGCTA CATTGACCTT GGGCGGGAGC TGAGTACACT TCATGCCCTG
    CTTTGGGAAG TGATCCCACA ACTTAGTAAG GATGCACTTA TAAAGTTGGG GCCTCTTCCA
    CGCCTCCTTA ATGACATCAG CCTAGCTCTC AGGAACCCCC ACATCCAACG ACAACCAAGC
    CACCAAGAAC GACTGCCACC CCCCAAACCT GTCATTCTGA GGGGACCTTC AGCTGAGCTG
    CAAAGCTACT TGGTGCGAGA CCTCAACAGC TCTATTGACA TGACTCGCCT ACCCTCACCT
    ACTAAGGATA AACCATCAAA AGATATGTTT TTTGTTTCAC GTCGCCCGCT AACTCGCTCC
    TCGCCAGCCT ATTGCACCAG CAGCTCAGAC ATCACAGAAC CAGACCAGAA GATGCTGAGC
    GTGAACAAGA GTGTGTCCAT GATGGACTTG CAGGACAGCC GAATGAATAG CGTAACCAAC
    TTGCAGGCCG TAGGTGATCT GCTGAACTCC AGCCAGGCCT CCATATCAGG GGGCCTACGT
    CCGGGGGGCC GGCTCTCCCA GGGCAGTGGA TCCAGCATTG CAGCGGGTCT GAGGCTCAGT
    CAGATGGGGG TCACCACTGA TGGGCTCTCA GCTCAACAGC TACGCATTCC CTTGTCCTTC
    CAGAACCCGC TCTTCCACAT GGCTCCCGAT GGGCCTGGTG GGCCCCCATC TCATCCTGCT
    CCTCATCGAC CAGACCCCGG AGCTGATGCC TTTGCCCCTT TCCATGGGTA TAGCAAGAGT
    GAGGACTTGA GCTCCGGAAA GCATATGCTT CACAGTCACA GCTACAGCGA CGAATTCAGC
    CGGCAGGGGT CAGAGTTCAG TAAAAGGCAG TTATCCCTCC AGGACAATCT CCAGCACATG
    CTTTCACCCC CTCAAATAAC CATTGGCCCT GGAGTGCCAC GAAGGCAAGA CTCACAGCCT
    CCCCCTTTGC AGAGGGGAAA ATCCCAACAA CTGACGGTGC AGGCAGCGCA TAAACCTCGG
    CCAAGCAGTG GCAACCTCCT ACAGAGCCCA GAACCAATGT ATGGGGCAGC AAGAATTAGG
    CAGCAGTCAG TTGAAAAGGA TGCAGGTGGC TTGAAACCAT CAATAACCAA GCAGCACTCC
    CATGCACAGG CCACTCTGAA CCCTGTCACT CCAGCCTCAG AACGCACAGT TGCCTGGGTA
    TCCAACATGC CCCACCTTTC AGCAGATATT GAGAGCGCTC ACATAGAACG GGAGGAGTAT
    AAGTTGAAGG AATACTCTAA ATCAATGGAT GAGAGCCGTC TTGAGAGGGT GAAAGAGTAT
    GAGGAAGAGA TTTACTCCTT AAAAGAGAGG CTGTTGATGT CAAACCGGAA GCTGGAGGAA
    TATGAGAGGC GTCTTCTTAC ACAGGAAGAA CAAACTAATA AGATGATGCA GCAATATCAG
    TCACGCCTGG ACCAGAGTGA AAAGCGTTTG CGACAGCAGC AGGTTGAGAA GGACACGCAG
    ATAAAGAGCA TCTTAAACAG GTTGATGCTG GTGGAGGAGG AGATCAGGAA GGATCACTCA
    CAAGAACCAA TCGATATGAA GAGGAGACTT ATCGAGGCTC AGGAGAAGAA GTTTCCCTCC
    GTGGGTACAG CAGACCCGCG TCTGAGGCTG GCGACTCCAT GGAATGGGGC CCTCCCGCCT
    CTGCCTCCTC GACTCCAAAT CACAGAAAAT GGAGACTTTC TAAACTCAAG TGACCATCAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031748231.1
    CDS665..4327
    Translation

    Target ORF information:

    RefSeq Version XM_031892371.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031892371.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGATGTTCT ATGCTCCCTT CAGAGATGCT CGAGGACCTC CCATGCATCG TATGCCCTAT 
    GTCACCTCTC CATACGACCG TCCAGCCTGG AACCCAAGAT ACTGTGTCCT CTCTGGAAAT
    CAGCTTCTCA TGCTTGATGA GGAGGAGGTT CACCCGCTGT TGATACGTGA AAGGAGAACT
    GATCCAACCA GAACAAAGTT CCTAAGGCGC ACAGTCAGTG TCCCCGTGGA GGGGAAGCAG
    CCTGACGAAC ACGATTATCA CCTTGCACGT TCCCGGAGGA AAAGCGTCCC AGGGGGAAAA
    CAGCCCAGTA TGGACGCCCC TCCCACCGCC CCCTTTCGAC CGTCGCAAGG ATTCCTGAGC
    CGTCGCTTGA AGAGCTCTAT AAAGAGGACA AAGAGTCAGC CCAAATTAGA TAGGACCAGC
    AGTTTCCGGC AAATATTGCC AAGGTTTCGA AGTGCTGATC ATGATAGGGC ACGGCTCATG
    CAGGCCTTTA AAGAGTCACA CTCTCACGAG TCCTTGCTTA GTCCTAGCAG TGCTGCAGAG
    GCTTTGGATT TGAACTTGGA TGAGGATTCT GTGGTGAAGC CTGTACACAG CAGCATCTTG
    GGACAGGAGT TTTGCTTCGA GGTGACCACA TCATCAGGTA CCAAATGTTT TGCCTGCCGT
    TCTGCTGCTG AAAGAGACAA ATGGATTGAG AATCTTCAAA GAGCTGTAAA GCCCAATAAG
    GATAACAGCA GGCGGGTAGA TAATGTCTTA AAGCTGTGGA TTATTGAAGC CCGTGATCTT
    CCCCCCAAGA AGCGCTATTA TTGTGAGCTC TGTTTGGATG ACATTTTGTA TGCCCGTACC
    ACCAGCAAGC CTCGGAATGA CACTGTCTTT TGGGGGGAAC ACTTTGAATT TAATAACCTT
    CCAGCTGTTC GGAACTTGCG CTTGCACCTC TATAAGGACA CAGACAAGAA AAGGCGCAAA
    GAAAAGAGCA ACTATGTGGG CATGGTGAAC GTGCCTATTG CCAGCATTAG TGGGCGCCAC
    TTTGTGGAGC AGTGGTACCC GGTTTCTCAG CCCACATCCT ACAAGGGGAA GAGCAGCTGT
    CCCTCCATTC GCCTAAAATC CCGATACCAA ACCATGAGCA TCCTCCCCAT GGAGCTGTAC
    AAGGAGTTTG CAGAGTATGT CACCAACAAC TACCGGATGC TGTGCGCTGT ACTGGAGCCT
    GTGCTTAGCG TGAAGAGCAA AGAGGAGGTT GCCTGTGCTT TAGTGCATAT ACTGCAAAGC
    ACAGGCAAGG CCAAGGACTT CCTTTCAGAC ATGGCTATGA CCGAGGTTGA TCGCTTCCTT
    GACAGAGAGC ACCTTATTCT CCGTGAGAAC ACACTTGCAA CTAAAGCCAT AGAGGAGTAT
    CTGAAACTGA TAGGACAGAG ATACCTCAAG GAAGCCATTG GTGAGTTTAT TCGGGCACTG
    TATGAGTCTG AGGAGGACTG TGAGGTTGAT CCCATGCGTT GTCCACCCTC TGCATTGCCC
    GATCACCAGG CCAATCTCAG GATGTGCTGT GAACTCGCCC TTTGCAAAGT CTTTAACTCT
    CATTGTGTGT TTCCTCGGGA GTTGAAGGAA GTCTTTGCAT CTTGGAGGAT GCGCTGTGCA
    GAGCGCGGCC GGGAAGATAT AGCAGATCGA CTCATCAGTG CCTCTCTCTT CCTCCGATTC
    CTGTGTCCGG CCATTATGTC CCCCAGTCTC TTCAGCCTAA TGCAGGAATA CCCTGATGAG
    CAAACATCTA GGACCCTCAC GCTCATCGCA AAAGTCATCC AGAACCTGGC CAACTTCAAC
    TACAACAGGT TCAGCAGTAA AGAAGAATAT ATGTCCTTTA TGAATGAGTT CCTAGAGACG
    GAGTTGGCGA ATATGCAGAA TTTCCTGTAT GAGATCTCAA ACCTGGATTC GCTCACCAAC
    AGCATTAACT TTGAGGGCTA CATTGACCTT GGGCGGGAGC TGAGTACACT TCATGCCCTG
    CTTTGGGAAG TGATCCCACA ACTTAGTAAG GATGCACTTA TAAAGTTGGG GCCTCTTCCA
    CGCCTCCTTA ATGACATCAG CCTAGCTCTC AGGAACCCCC ACATCCAACG ACAACCAAGC
    CACCAAGAAC GACTGCCACC CCCCAAACCT GTCATTCTGA GGGGACCTTC AGCTGAGCTG
    CAAAGCTACT TGGTGCGAGA CCTCAACAGC TCTATTGACA TGACTCGCCT ACCCTCACCT
    ACTAAGGATA AACCATCAAA AGATATGTTT TTTGTTTCAC GTCGCCCGCT AACTCGCTCC
    TCGCCAGCCT ATTGCACCAG CAGCTCAGAC ATCACAGAAC CAGACCAGAA GATGCTGAGC
    GTGAACAAGA GTGTGTCCAT GATGGACTTG CAGGACAGCC GAATGAATAG CGTAACCAAC
    TTGCAGGCCG TAGGTGATCT GCTGAACTCC AGCCAGGCCT CCATATCAGG GGGCCTACGT
    CCGGGGGGCC GGCTCTCCCA GGGCAGTGGA TCCAGCATTG CAGCGGGTCT GAGGCTCAGT
    CAGATGGGGG TCACCACTGA TGGGCTCTCA GCTCAACAGC TACGCATTCC CTTGTCCTTC
    CAGAACCCGC TCTTCCACAT GGCTCCCGAT GGGCCTGGTG GGCCCCCATC TCATCCTGCT
    CCTCATCGAC CAGACCCCGG AGCTGATGCC TTTGCCCCTT TCCATGGGTA TAGCAAGAGT
    GAGGACTTGA GCTCCGGAAA GCATATGCTT CACAGTCACA GCTACAGCGA CGAATTCAGC
    CGGCAGGGGT CAGAGTTCAG TAAAAGGCAG TTATCCCTCC AGGACAATCT CCAGCACATG
    CTTTCACCCC CTCAAATAAC CATTGGCCCT GGAGTGCCAC GAAGGCAAGA CTCACAGCCT
    CCCCCTTTGC AGAGGGGAAA ATCCCAACAA CTGACGGTGC AGGCAGCGCA TAAACCTCGG
    CCAAGCAGTG GCAACCTCCT ACAGAGCCCA GAACCAATGT ATGGGGCAGC AAGAATTAGG
    CAGCAGTCAG TTGAAAAGGA TGCAGGTGGC TTGAAACCAT CAATAACCAA GCAGCACTCC
    CATGCACAGG CCACTCTGAA CCCTGTCACT CCAGCCTCAG AACGCACAGT TGCCTGGGTA
    TCCAACATGC CCCACCTTTC AGCAGATATT GAGAGCGCTC ACATAGAACG GGAGGAGTAT
    AAGTTGAAGG AATACTCTAA ATCAATGGAT GAGAGCCGTC TTGAGAGGGT GAAAGAGTAT
    GAGGAAGAGA TTTACTCCTT AAAAGAGAGG CTGTTGATGT CAAACCGGAA GCTGGAGGAA
    TATGAGAGGC GTCTTCTTAC ACAGGAAGAA CAAACTAATA AGATGATGCA GCAATATCAG
    TCACGCCTGG ACCAGAGTGA AAAGCGTTTG CGACAGCAGC AGGTTGAGAA GGACACGCAG
    ATAAAGAGCA TCTTAAACAG GTTGATGCTG GTGGAGGAGG AGATCAGGAA GGATCACTCA
    CAAGAACCAA TCGATATGAA GAGGAGACTT ATCGAGGCTC AGGAGAAGAA GTTTCCCTCC
    GTGGGTACAG CAGACCCGCG TCTGAGGCTG GCGACTCCAT GGAATGGGGC CCTCCCGCCT
    CTGCCTCCTC GACTCCAAAT CACAGAAAAT GGAGACTTTC TAAACTCAAG TGACCATCAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa65727
    Clone ID Related Accession (Same CDS sequence) XM_031892372.1
    Accession Version XM_031892372.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3660bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ras/Rap GTPase-activating protein SynGAP isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGATGTTCT ATGCTCCCTT CAGAGATGCT CGAGGACCTC CCATGCATCG TATGCCCTAT 
    GTCACCTCTC CATACGACCG TCCAGCCTGG AACCCAAGAT ACTGTGTCCT CTCTGGAAAT
    CAGCTTCTCA TGCTTGATGA GGAGGAGGTT CACCCGCTGT TGATACGTGA AAGGAGAACT
    GATCCAACCA GAACAAAGTT CCTAAGGCGC ACAGTCAGTG TCCCCGTGGA GGGGAAGCAG
    CCTGACGAAC ACGATTATCA CCTTGCACGT TCCCGGAGGA AAAGCGTCCC AGGGGGAAAA
    CAGCCCAGTA TGGACGCCCC TCCCACCGCC CCCTTTCGAC CGTCGCAAGG ATTCCTGAGC
    CGTCGCTTGA AGAGCTCTAT AAAGAGGACA AAGAGTCAGC CCAAATTAGA TAGGACCAGC
    AGTTTCCGGC AAATATTGCC AAGGTTTCGA AGTGCTGATC ATGATAGGGC ACGGCTCATG
    CAGGCCTTTA AAGAGTCACA CTCTCACGAG TCCTTGCTTA GTCCTAGCAG TGCTGCAGAG
    GCTTTGGATT TGAACTTGGA TGAGGATTCT GTGGTGAAGC CTGTACACAG CAGCATCTTG
    GGACAGGAGT TTTGCTTCGA GGTGACCACA TCATCAGGTA CCAAATGTTT TGCCTGCCGT
    TCTGCTGCTG AAAGAGACAA ATGGATTGAG AATCTTCAAA GAGCTGTAAA GCCCAATAAG
    GATAACAGCA GGCGGGTAGA TAATGTCTTA AAGCTGTGGA TTATTGAAGC CCGTGATCTT
    CCCCCCAAGA AGCGCTATTA TTGTGAGCTC TGTTTGGATG ACATTTTGTA TGCCCGTACC
    ACCAGCAAGC CTCGGAATGA CACTGTCTTT TGGGGGGAAC ACTTTGAATT TAATAACCTT
    CCAGCTGTTC GGAACTTGCG CTTGCACCTC TATAAGGACA CAGACAAGAA AAGGCGCAAA
    GAAAAGAGCA ACTATGTGGG CATGGTGAAC GTGCCTATTG CCAGCATTAG TGGGCGCCAC
    TTTGTGGAGC AGTGGTACCC GGTTTCTCAG CCCACATCCT ACAAGGGGAA GAGCAGCTGT
    CCCTCCATTC GCCTAAAATC CCGATACCAA ACCATGAGCA TCCTCCCCAT GGAGCTGTAC
    AAGGAGTTTG CAGAGTATGT CACCAACAAC TACCGGATGC TGTGCGCTGT ACTGGAGCCT
    GTGCTTAGCG TGAAGAGCAA AGAGGAGGTT GCCTGTGCTT TAGTGCATAT ACTGCAAAGC
    ACAGGCAAGG CCAAGGACTT CCTTTCAGAC ATGGCTATGA CCGAGGTTGA TCGCTTCCTT
    GACAGAGAGC ACCTTATTCT CCGTGAGAAC ACACTTGCAA CTAAAGCCAT AGAGGAGTAT
    CTGAAACTGA TAGGACAGAG ATACCTCAAG GAAGCCATTG GTGAGTTTAT TCGGGCACTG
    TATGAGTCTG AGGAGGACTG TGAGGTTGAT CCCATGCGTT GTCCACCCTC TGCATTGCCC
    GATCACCAGG CCAATCTCAG GATGTGCTGT GAACTCGCCC TTTGCAAAGT CTTTAACTCT
    CATTGTGTGT TTCCTCGGGA GTTGAAGGAA GTCTTTGCAT CTTGGAGGAT GCGCTGTGCA
    GAGCGCGGCC GGGAAGATAT AGCAGATCGA CTCATCAGTG CCTCTCTCTT CCTCCGATTC
    CTGTGTCCGG CCATTATGTC CCCCAGTCTC TTCAGCCTAA TGCAGGAATA CCCTGATGAG
    CAAACATCTA GGACCCTCAC GCTCATCGCA AAAGTCATCC AGAACCTGGC CAACTTCAAC
    TACAACAGGT TCAGCAGTAA AGAAGAATAT ATGTCCTTTA TGAATGAGTT CCTAGAGACG
    GAGTTGGCGA ATATGCAGAA TTTCCTGTAT GAGATCTCAA ACCTGGATTC GCTCACCAAC
    AGCATTAACT TTGAGGGCTA CATTGACCTT GGGCGGGAGC TGAGTACACT TCATGCCCTG
    CTTTGGGAAG TGATCCCACA ACTTAGTAAG GATGCACTTA TAAAGTTGGG GCCTCTTCCA
    CGCCTCCTTA ATGACATCAG CCTAGCTCTC AGGAACCCCC ACATCCAACG ACAACCAAGC
    CACCAAGAAC GACTGCCACC CCCCAAACCT GTCATTCTGA GGGGACCTTC AGCTGAGCTG
    CAAAGCTACT TGGTGCGAGA CCTCAACAGC TCTATTGACA TGACTCGCCT ACCCTCACCT
    ACTAAGGATA AACCATCAAA AGATATGTTT TTTGTTTCAC GTCGCCCGCT AACTCGCTCC
    TCGCCAGCCT ATTGCACCAG CAGCTCAGAC ATCACAGAAC CAGACCAGAA GATGCTGAGC
    GTGAACAAGA GTGTGTCCAT GATGGACTTG CAGGACAGCC GAATGAATAG CGTAACCAAC
    TTGCAGGCCG TAGGTGATCT GCTGAACTCC AGCCAGGCCT CCATATCAGG GGGCCTACGT
    CCGGGGGGCC GGCTCTCCCA GGGCAGTGGA TCCAGCATTG CAGCGGGTCT GAGGCTCAGT
    CAGATGGGGG TCACCACTGA TGGGCTCTCA GCTCAACAGC TACGCATTCC CTTGTCCTTC
    CAGAACCCGC TCTTCCACAT GGCTCCCGAT GGGCCTGGTG GGCCCCCATC TCATCCTGCT
    CCTCATCGAC CAGACCCCGG AGCTGATGCC TTTGCCCCTT TCCATGGGTA TAGCAAGAGT
    GAGGACTTGA GCTCCGGAAA GCATATGCTT CACAGTCACA GCTACAGCGA CGAATTCAGC
    CGGCAGGGTA AAAGGCAGTT ATCCCTCCAG GACAATCTCC AGCACATGCT TTCACCCCCT
    CAAATAACCA TTGGCCCTGG AGTGCCACGA AGGCAAGACT CACAGCCTCC CCCTTTGCAG
    AGGGGAAAAT CCCAACAACT GACGGTGCAG GCAGCGCATA AACCTCGGCC AAGCAGTGGC
    AACCTCCTAC AGAGCCCAGA ACCAATGTAT GGGGCAGCAA GAATTAGGCA GCAGTCAGTT
    GAAAAGGATG CAGGTGGCTT GAAACCATCA ATAACCAAGC AGCACTCCCA TGCACAGGCC
    ACTCTGAACC CTGTCACTCC AGCCTCAGAA CGCACAGTTG CCTGGGTATC CAACATGCCC
    CACCTTTCAG CAGATATTGA GAGCGCTCAC ATAGAACGGG AGGAGTATAA GTTGAAGGAA
    TACTCTAAAT CAATGGATGA GAGCCGTCTT GAGAGGGTGA AAGAGTATGA GGAAGAGATT
    TACTCCTTAA AAGAGAGGCT GTTGATGTCA AACCGGAAGC TGGAGGAATA TGAGAGGCGT
    CTTCTTACAC AGGAAGAACA AACTAATAAG ATGATGCAGC AATATCAGTC ACGCCTGGAC
    CAGAGTGAAA AGCGTTTGCG ACAGCAGCAG GTTGAGAAGG ACACGCAGAT AAAGAGCATC
    TTAAACAGGT TAGAAATGGT TGATGCTGGT GGAGGAGGAG ATCAGGAAGG ATCACTCACA
    AGAACCAATC GATATGAAGA GGAGACTTAT CGAGGCTCAG AGAAGAAGTT TCCCTCCGTG
    GGTACAGCAG ACCCGCGTCT GAGGCTGGCG ACTCCATGGA ATGGGGCCCT CCCGCCTCTG
    CCTCCTCGAC TCCAAATCAC AGAAAATGGA GACTTTCTAA ACTCAAGTGA CCATCACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031748232.1
    CDS665..4324
    Translation

    Target ORF information:

    RefSeq Version XM_031892372.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031892372.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGATGTTCT ATGCTCCCTT CAGAGATGCT CGAGGACCTC CCATGCATCG TATGCCCTAT 
    GTCACCTCTC CATACGACCG TCCAGCCTGG AACCCAAGAT ACTGTGTCCT CTCTGGAAAT
    CAGCTTCTCA TGCTTGATGA GGAGGAGGTT CACCCGCTGT TGATACGTGA AAGGAGAACT
    GATCCAACCA GAACAAAGTT CCTAAGGCGC ACAGTCAGTG TCCCCGTGGA GGGGAAGCAG
    CCTGACGAAC ACGATTATCA CCTTGCACGT TCCCGGAGGA AAAGCGTCCC AGGGGGAAAA
    CAGCCCAGTA TGGACGCCCC TCCCACCGCC CCCTTTCGAC CGTCGCAAGG ATTCCTGAGC
    CGTCGCTTGA AGAGCTCTAT AAAGAGGACA AAGAGTCAGC CCAAATTAGA TAGGACCAGC
    AGTTTCCGGC AAATATTGCC AAGGTTTCGA AGTGCTGATC ATGATAGGGC ACGGCTCATG
    CAGGCCTTTA AAGAGTCACA CTCTCACGAG TCCTTGCTTA GTCCTAGCAG TGCTGCAGAG
    GCTTTGGATT TGAACTTGGA TGAGGATTCT GTGGTGAAGC CTGTACACAG CAGCATCTTG
    GGACAGGAGT TTTGCTTCGA GGTGACCACA TCATCAGGTA CCAAATGTTT TGCCTGCCGT
    TCTGCTGCTG AAAGAGACAA ATGGATTGAG AATCTTCAAA GAGCTGTAAA GCCCAATAAG
    GATAACAGCA GGCGGGTAGA TAATGTCTTA AAGCTGTGGA TTATTGAAGC CCGTGATCTT
    CCCCCCAAGA AGCGCTATTA TTGTGAGCTC TGTTTGGATG ACATTTTGTA TGCCCGTACC
    ACCAGCAAGC CTCGGAATGA CACTGTCTTT TGGGGGGAAC ACTTTGAATT TAATAACCTT
    CCAGCTGTTC GGAACTTGCG CTTGCACCTC TATAAGGACA CAGACAAGAA AAGGCGCAAA
    GAAAAGAGCA ACTATGTGGG CATGGTGAAC GTGCCTATTG CCAGCATTAG TGGGCGCCAC
    TTTGTGGAGC AGTGGTACCC GGTTTCTCAG CCCACATCCT ACAAGGGGAA GAGCAGCTGT
    CCCTCCATTC GCCTAAAATC CCGATACCAA ACCATGAGCA TCCTCCCCAT GGAGCTGTAC
    AAGGAGTTTG CAGAGTATGT CACCAACAAC TACCGGATGC TGTGCGCTGT ACTGGAGCCT
    GTGCTTAGCG TGAAGAGCAA AGAGGAGGTT GCCTGTGCTT TAGTGCATAT ACTGCAAAGC
    ACAGGCAAGG CCAAGGACTT CCTTTCAGAC ATGGCTATGA CCGAGGTTGA TCGCTTCCTT
    GACAGAGAGC ACCTTATTCT CCGTGAGAAC ACACTTGCAA CTAAAGCCAT AGAGGAGTAT
    CTGAAACTGA TAGGACAGAG ATACCTCAAG GAAGCCATTG GTGAGTTTAT TCGGGCACTG
    TATGAGTCTG AGGAGGACTG TGAGGTTGAT CCCATGCGTT GTCCACCCTC TGCATTGCCC
    GATCACCAGG CCAATCTCAG GATGTGCTGT GAACTCGCCC TTTGCAAAGT CTTTAACTCT
    CATTGTGTGT TTCCTCGGGA GTTGAAGGAA GTCTTTGCAT CTTGGAGGAT GCGCTGTGCA
    GAGCGCGGCC GGGAAGATAT AGCAGATCGA CTCATCAGTG CCTCTCTCTT CCTCCGATTC
    CTGTGTCCGG CCATTATGTC CCCCAGTCTC TTCAGCCTAA TGCAGGAATA CCCTGATGAG
    CAAACATCTA GGACCCTCAC GCTCATCGCA AAAGTCATCC AGAACCTGGC CAACTTCAAC
    TACAACAGGT TCAGCAGTAA AGAAGAATAT ATGTCCTTTA TGAATGAGTT CCTAGAGACG
    GAGTTGGCGA ATATGCAGAA TTTCCTGTAT GAGATCTCAA ACCTGGATTC GCTCACCAAC
    AGCATTAACT TTGAGGGCTA CATTGACCTT GGGCGGGAGC TGAGTACACT TCATGCCCTG
    CTTTGGGAAG TGATCCCACA ACTTAGTAAG GATGCACTTA TAAAGTTGGG GCCTCTTCCA
    CGCCTCCTTA ATGACATCAG CCTAGCTCTC AGGAACCCCC ACATCCAACG ACAACCAAGC
    CACCAAGAAC GACTGCCACC CCCCAAACCT GTCATTCTGA GGGGACCTTC AGCTGAGCTG
    CAAAGCTACT TGGTGCGAGA CCTCAACAGC TCTATTGACA TGACTCGCCT ACCCTCACCT
    ACTAAGGATA AACCATCAAA AGATATGTTT TTTGTTTCAC GTCGCCCGCT AACTCGCTCC
    TCGCCAGCCT ATTGCACCAG CAGCTCAGAC ATCACAGAAC CAGACCAGAA GATGCTGAGC
    GTGAACAAGA GTGTGTCCAT GATGGACTTG CAGGACAGCC GAATGAATAG CGTAACCAAC
    TTGCAGGCCG TAGGTGATCT GCTGAACTCC AGCCAGGCCT CCATATCAGG GGGCCTACGT
    CCGGGGGGCC GGCTCTCCCA GGGCAGTGGA TCCAGCATTG CAGCGGGTCT GAGGCTCAGT
    CAGATGGGGG TCACCACTGA TGGGCTCTCA GCTCAACAGC TACGCATTCC CTTGTCCTTC
    CAGAACCCGC TCTTCCACAT GGCTCCCGAT GGGCCTGGTG GGCCCCCATC TCATCCTGCT
    CCTCATCGAC CAGACCCCGG AGCTGATGCC TTTGCCCCTT TCCATGGGTA TAGCAAGAGT
    GAGGACTTGA GCTCCGGAAA GCATATGCTT CACAGTCACA GCTACAGCGA CGAATTCAGC
    CGGCAGGGTA AAAGGCAGTT ATCCCTCCAG GACAATCTCC AGCACATGCT TTCACCCCCT
    CAAATAACCA TTGGCCCTGG AGTGCCACGA AGGCAAGACT CACAGCCTCC CCCTTTGCAG
    AGGGGAAAAT CCCAACAACT GACGGTGCAG GCAGCGCATA AACCTCGGCC AAGCAGTGGC
    AACCTCCTAC AGAGCCCAGA ACCAATGTAT GGGGCAGCAA GAATTAGGCA GCAGTCAGTT
    GAAAAGGATG CAGGTGGCTT GAAACCATCA ATAACCAAGC AGCACTCCCA TGCACAGGCC
    ACTCTGAACC CTGTCACTCC AGCCTCAGAA CGCACAGTTG CCTGGGTATC CAACATGCCC
    CACCTTTCAG CAGATATTGA GAGCGCTCAC ATAGAACGGG AGGAGTATAA GTTGAAGGAA
    TACTCTAAAT CAATGGATGA GAGCCGTCTT GAGAGGGTGA AAGAGTATGA GGAAGAGATT
    TACTCCTTAA AAGAGAGGCT GTTGATGTCA AACCGGAAGC TGGAGGAATA TGAGAGGCGT
    CTTCTTACAC AGGAAGAACA AACTAATAAG ATGATGCAGC AATATCAGTC ACGCCTGGAC
    CAGAGTGAAA AGCGTTTGCG ACAGCAGCAG GTTGAGAAGG ACACGCAGAT AAAGAGCATC
    TTAAACAGGT TAGAAATGGT TGATGCTGGT GGAGGAGGAG ATCAGGAAGG ATCACTCACA
    AGAACCAATC GATATGAAGA GGAGACTTAT CGAGGCTCAG AGAAGAAGTT TCCCTCCGTG
    GGTACAGCAG ACCCGCGTCT GAGGCTGGCG ACTCCATGGA ATGGGGCCCT CCCGCCTCTG
    CCTCCTCGAC TCCAAATCAC AGAAAATGGA GACTTTCTAA ACTCAAGTGA CCATCACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa65728
    Clone ID Related Accession (Same CDS sequence) XM_031892373.1
    Accession Version XM_031892373.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3660bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ras/Rap GTPase-activating protein SynGAP isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGATGTTCT ATGCTCCCTT CAGAGATGCT CGAGGACCTC CCATGCATCG TATGCCCTAT 
    GTCACCTCTC CATACGACCG TCCAGCCTGG AACCCAAGAT ACTGTGTCCT CTCTGGAAAT
    CAGCTTCTCA TGCTTGATGA GGAGGAGGTT CACCCGCTGT TGATACGTGA AAGGAGAACT
    GATCCAACCA GAACAAAGTT CCTAAGGCGC ACAGTCAGTG TCCCCGTGGA GGGGAAGCAG
    CCTGACGAAC ACGATTATCA CCTTGCACGT TCCCGGAGGA AAAGCGTCCC AGGGGGAAAA
    CAGCCCAGTA TGGACGCCCC TCCCACCGCC CCCTTTCGAC CGTCGCAAGG ATTCCTGAGC
    CGTCGCTTGA AGAGCTCTAT AAAGAGGACA AAGAGTCAGC CCAAATTAGA TAGGACCAGC
    AGTTTCCGGC AAATATTGCC AAGGTTTCGA AGTGCTGATC ATGATAGGGC ACGGCTCATG
    CAGGCCTTTA AAGAGTCACA CTCTCACGAG TCCTTGCTTA GTCCTAGCAG TGCTGCAGAG
    GCTTTGGATT TGAACTTGGA TGAGGATTCT GTGGTGAAGC CTGTACACAG CAGCATCTTG
    GGACAGGAGT TTTGCTTCGA GGTGACCACA TCATCAGGTA CCAAATGTTT TGCCTGCCGT
    TCTGCTGCTG AAAGAGACAA ATGGATTGAG AATCTTCAAA GAGCTGTAAA GCCCAATAAG
    GATAACAGCA GGCGGGTAGA TAATGTCTTA AAGCTGTGGA TTATTGAAGC CCGTGATCTT
    CCCCCCAAGA AGCGCTATTA TTGTGAGCTC TGTTTGGATG ACATTTTGTA TGCCCGTACC
    ACCAGCAAGC CTCGGAATGA CACTGTCTTT TGGGGGGAAC ACTTTGAATT TAATAACCTT
    CCAGCTGTTC GGAACTTGCG CTTGCACCTC TATAAGGACA CAGACAAGAA AAGGCGCAAA
    GAAAAGAGCA ACTATGTGGG CATGGTGAAC GTGCCTATTG CCAGCATTAG TGGGCGCCAC
    TTTGTGGAGC AGTGGTACCC GGTTTCTCAG CCCACATCCT ACAAGGGGAA GAGCAGCTGT
    CCCTCCATTC GCCTAAAATC CCGATACCAA ACCATGAGCA TCCTCCCCAT GGAGCTGTAC
    AAGGAGTTTG CAGAGTATGT CACCAACAAC TACCGGATGC TGTGCGCTGT ACTGGAGCCT
    GTGCTTAGCG TGAAGAGCAA AGAGGAGGTT GCCTGTGCTT TAGTGCATAT ACTGCAAAGC
    ACAGGCAAGG CCAAGGACTT CCTTTCAGAC ATGGCTATGA CCGAGGTTGA TCGCTTCCTT
    GACAGAGAGC ACCTTATTCT CCGTGAGAAC ACACTTGCAA CTAAAGCCAT AGAGGAGTAT
    CTGAAACTGA TAGGACAGAG ATACCTCAAG GAAGCCATTG GTGAGTTTAT TCGGGCACTG
    TATGAGTCTG AGGAGGACTG TGAGGTTGAT CCCATGCGTT GTCCACCCTC TGCATTGCCC
    GATCACCAGG CCAATCTCAG GATGTGCTGT GAACTCGCCC TTTGCAAAGT CTTTAACTCT
    CATTGTGTGT TTCCTCGGGA GTTGAAGGAA GTCTTTGCAT CTTGGAGGAT GCGCTGTGCA
    GAGCGCGGCC GGGAAGATAT AGCAGATCGA CTCATCAGTG CCTCTCTCTT CCTCCGATTC
    CTGTGTCCGG CCATTATGTC CCCCAGTCTC TTCAGCCTAA TGCAGGAATA CCCTGATGAG
    CAAACATCTA GGACCCTCAC GCTCATCGCA AAAGTCATCC AGAACCTGGC CAACTTCAAC
    TACAAGTTCA GCAGTAAAGA AGAATATATG TCCTTTATGA ATGAGTTCCT AGAGACGGAG
    TTGGCGAATA TGCAGAATTT CCTGTATGAG ATCTCAAACC TGGATTCGCT CACCAACAGC
    ATTAACTTTG AGGGCTACAT TGACCTTGGG CGGGAGCTGA GTACACTTCA TGCCCTGCTT
    TGGGAAGTGA TCCCACAACT TAGTAAGGAT GCACTTATAA AGTTGGGGCC TCTTCCACGC
    CTCCTTAATG ACATCAGCCT AGCTCTCAGG AACCCCCACA TCCAACGACA ACCAAGCCAC
    CAAGAACGAC TGCCACCCCC CAAACCTGTC ATTCTGAGGG GACCTTCAGC TGAGCTGCAA
    AGCTACTTGG TGCGAGACCT CAACAGCTCT ATTGACATGA CTCGCCTACC CTCACCTACT
    AAGGATAAAC CATCAAAAGA TATGTTTTTT GTTTCACGTC GCCCGCTAAC TCGCTCCTCG
    CCAGCCTATT GCACCAGCAG CTCAGACATC ACAGAACCAG ACCAGAAGAT GCTGAGCGTG
    AACAAGAGTG TGTCCATGAT GGACTTGCAG GACAGCCGAA TGAATAGCGT AACCAACTTG
    CAGGCCGTAG GTGATCTGCT GAACTCCAGC CAGGCCTCCA TATCAGGGGG CCTACGTCCG
    GGGGGCCGGC TCTCCCAGGG CAGTGGATCC AGCATTGCAG CGGGTCTGAG GCTCAGTCAG
    ATGGGGGTCA CCACTGATGG GCTCTCAGCT CAACAGCTAC GCATTCCCTT GTCCTTCCAG
    AACCCGCTCT TCCACATGGC TCCCGATGGG CCTGGTGGGC CCCCATCTCA TCCTGCTCCT
    CATCGACCAG ACCCCGGAGC TGATGCCTTT GCCCCTTTCC ATGGGTATAG CAAGAGTGAG
    GACTTGAGCT CCGGAAAGCA TATGCTTCAC AGTCACAGCT ACAGCGACGA ATTCAGCCGG
    CAGGGGTCAG AGTTCAGTAA AAGGCAGTTA TCCCTCCAGG ACAATCTCCA GCACATGCTT
    TCACCCCCTC AAATAACCAT TGGCCCTGGA GTGCCACGAA GGCAAGACTC ACAGCCTCCC
    CCTTTGCAGA GGGGAAAATC CCAACAACTG ACGGTGCAGG CAGCGCATAA ACCTCGGCCA
    AGCAGTGGCA ACCTCCTACA GAGCCCAGAA CCAATGTATG GGGCAGCAAG AATTAGGCAG
    CAGTCAGTTG AAAAGGATGC AGGTGGCTTG AAACCATCAA TAACCAAGCA GCACTCCCAT
    GCACAGGCCA CTCTGAACCC TGTCACTCCA GCCTCAGAAC GCACAGTTGC CTGGGTATCC
    AACATGCCCC ACCTTTCAGC AGATATTGAG AGCGCTCACA TAGAACGGGA GGAGTATAAG
    TTGAAGGAAT ACTCTAAATC AATGGATGAG AGCCGTCTTG AGAGGGTGAA AGAGTATGAG
    GAAGAGATTT ACTCCTTAAA AGAGAGGCTG TTGATGTCAA ACCGGAAGCT GGAGGAATAT
    GAGAGGCGTC TTCTTACACA GGAAGAACAA ACTAATAAGA TGATGCAGCA ATATCAGTCA
    CGCCTGGACC AGAGTGAAAA GCGTTTGCGA CAGCAGCAGG TTGAGAAGGA CACGCAGATA
    AAGAGCATCT TAAACAGGTT GATGCTGGTG GAGGAGGAGA TCAGGAAGGA TCACTCACAA
    GAACCAATCG ATATGAAGAG GAGACTTATC GAGGCTCAGG AGAAGAAGTT TCCCTCCGTG
    GGTACAGCAG ACCCGCGTCT GAGGCTGGCG ACTCCATGGA ATGGGGCCCT CCCGCCTCTG
    CCTCCTCGAC TCCAAATCAC AGAAAATGGA GACTTTCTAA ACTCAAGTGA CCATCACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031748233.1
    CDS665..4324
    Translation

    Target ORF information:

    RefSeq Version XM_031892373.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031892373.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGATGTTCT ATGCTCCCTT CAGAGATGCT CGAGGACCTC CCATGCATCG TATGCCCTAT 
    GTCACCTCTC CATACGACCG TCCAGCCTGG AACCCAAGAT ACTGTGTCCT CTCTGGAAAT
    CAGCTTCTCA TGCTTGATGA GGAGGAGGTT CACCCGCTGT TGATACGTGA AAGGAGAACT
    GATCCAACCA GAACAAAGTT CCTAAGGCGC ACAGTCAGTG TCCCCGTGGA GGGGAAGCAG
    CCTGACGAAC ACGATTATCA CCTTGCACGT TCCCGGAGGA AAAGCGTCCC AGGGGGAAAA
    CAGCCCAGTA TGGACGCCCC TCCCACCGCC CCCTTTCGAC CGTCGCAAGG ATTCCTGAGC
    CGTCGCTTGA AGAGCTCTAT AAAGAGGACA AAGAGTCAGC CCAAATTAGA TAGGACCAGC
    AGTTTCCGGC AAATATTGCC AAGGTTTCGA AGTGCTGATC ATGATAGGGC ACGGCTCATG
    CAGGCCTTTA AAGAGTCACA CTCTCACGAG TCCTTGCTTA GTCCTAGCAG TGCTGCAGAG
    GCTTTGGATT TGAACTTGGA TGAGGATTCT GTGGTGAAGC CTGTACACAG CAGCATCTTG
    GGACAGGAGT TTTGCTTCGA GGTGACCACA TCATCAGGTA CCAAATGTTT TGCCTGCCGT
    TCTGCTGCTG AAAGAGACAA ATGGATTGAG AATCTTCAAA GAGCTGTAAA GCCCAATAAG
    GATAACAGCA GGCGGGTAGA TAATGTCTTA AAGCTGTGGA TTATTGAAGC CCGTGATCTT
    CCCCCCAAGA AGCGCTATTA TTGTGAGCTC TGTTTGGATG ACATTTTGTA TGCCCGTACC
    ACCAGCAAGC CTCGGAATGA CACTGTCTTT TGGGGGGAAC ACTTTGAATT TAATAACCTT
    CCAGCTGTTC GGAACTTGCG CTTGCACCTC TATAAGGACA CAGACAAGAA AAGGCGCAAA
    GAAAAGAGCA ACTATGTGGG CATGGTGAAC GTGCCTATTG CCAGCATTAG TGGGCGCCAC
    TTTGTGGAGC AGTGGTACCC GGTTTCTCAG CCCACATCCT ACAAGGGGAA GAGCAGCTGT
    CCCTCCATTC GCCTAAAATC CCGATACCAA ACCATGAGCA TCCTCCCCAT GGAGCTGTAC
    AAGGAGTTTG CAGAGTATGT CACCAACAAC TACCGGATGC TGTGCGCTGT ACTGGAGCCT
    GTGCTTAGCG TGAAGAGCAA AGAGGAGGTT GCCTGTGCTT TAGTGCATAT ACTGCAAAGC
    ACAGGCAAGG CCAAGGACTT CCTTTCAGAC ATGGCTATGA CCGAGGTTGA TCGCTTCCTT
    GACAGAGAGC ACCTTATTCT CCGTGAGAAC ACACTTGCAA CTAAAGCCAT AGAGGAGTAT
    CTGAAACTGA TAGGACAGAG ATACCTCAAG GAAGCCATTG GTGAGTTTAT TCGGGCACTG
    TATGAGTCTG AGGAGGACTG TGAGGTTGAT CCCATGCGTT GTCCACCCTC TGCATTGCCC
    GATCACCAGG CCAATCTCAG GATGTGCTGT GAACTCGCCC TTTGCAAAGT CTTTAACTCT
    CATTGTGTGT TTCCTCGGGA GTTGAAGGAA GTCTTTGCAT CTTGGAGGAT GCGCTGTGCA
    GAGCGCGGCC GGGAAGATAT AGCAGATCGA CTCATCAGTG CCTCTCTCTT CCTCCGATTC
    CTGTGTCCGG CCATTATGTC CCCCAGTCTC TTCAGCCTAA TGCAGGAATA CCCTGATGAG
    CAAACATCTA GGACCCTCAC GCTCATCGCA AAAGTCATCC AGAACCTGGC CAACTTCAAC
    TACAAGTTCA GCAGTAAAGA AGAATATATG TCCTTTATGA ATGAGTTCCT AGAGACGGAG
    TTGGCGAATA TGCAGAATTT CCTGTATGAG ATCTCAAACC TGGATTCGCT CACCAACAGC
    ATTAACTTTG AGGGCTACAT TGACCTTGGG CGGGAGCTGA GTACACTTCA TGCCCTGCTT
    TGGGAAGTGA TCCCACAACT TAGTAAGGAT GCACTTATAA AGTTGGGGCC TCTTCCACGC
    CTCCTTAATG ACATCAGCCT AGCTCTCAGG AACCCCCACA TCCAACGACA ACCAAGCCAC
    CAAGAACGAC TGCCACCCCC CAAACCTGTC ATTCTGAGGG GACCTTCAGC TGAGCTGCAA
    AGCTACTTGG TGCGAGACCT CAACAGCTCT ATTGACATGA CTCGCCTACC CTCACCTACT
    AAGGATAAAC CATCAAAAGA TATGTTTTTT GTTTCACGTC GCCCGCTAAC TCGCTCCTCG
    CCAGCCTATT GCACCAGCAG CTCAGACATC ACAGAACCAG ACCAGAAGAT GCTGAGCGTG
    AACAAGAGTG TGTCCATGAT GGACTTGCAG GACAGCCGAA TGAATAGCGT AACCAACTTG
    CAGGCCGTAG GTGATCTGCT GAACTCCAGC CAGGCCTCCA TATCAGGGGG CCTACGTCCG
    GGGGGCCGGC TCTCCCAGGG CAGTGGATCC AGCATTGCAG CGGGTCTGAG GCTCAGTCAG
    ATGGGGGTCA CCACTGATGG GCTCTCAGCT CAACAGCTAC GCATTCCCTT GTCCTTCCAG
    AACCCGCTCT TCCACATGGC TCCCGATGGG CCTGGTGGGC CCCCATCTCA TCCTGCTCCT
    CATCGACCAG ACCCCGGAGC TGATGCCTTT GCCCCTTTCC ATGGGTATAG CAAGAGTGAG
    GACTTGAGCT CCGGAAAGCA TATGCTTCAC AGTCACAGCT ACAGCGACGA ATTCAGCCGG
    CAGGGGTCAG AGTTCAGTAA AAGGCAGTTA TCCCTCCAGG ACAATCTCCA GCACATGCTT
    TCACCCCCTC AAATAACCAT TGGCCCTGGA GTGCCACGAA GGCAAGACTC ACAGCCTCCC
    CCTTTGCAGA GGGGAAAATC CCAACAACTG ACGGTGCAGG CAGCGCATAA ACCTCGGCCA
    AGCAGTGGCA ACCTCCTACA GAGCCCAGAA CCAATGTATG GGGCAGCAAG AATTAGGCAG
    CAGTCAGTTG AAAAGGATGC AGGTGGCTTG AAACCATCAA TAACCAAGCA GCACTCCCAT
    GCACAGGCCA CTCTGAACCC TGTCACTCCA GCCTCAGAAC GCACAGTTGC CTGGGTATCC
    AACATGCCCC ACCTTTCAGC AGATATTGAG AGCGCTCACA TAGAACGGGA GGAGTATAAG
    TTGAAGGAAT ACTCTAAATC AATGGATGAG AGCCGTCTTG AGAGGGTGAA AGAGTATGAG
    GAAGAGATTT ACTCCTTAAA AGAGAGGCTG TTGATGTCAA ACCGGAAGCT GGAGGAATAT
    GAGAGGCGTC TTCTTACACA GGAAGAACAA ACTAATAAGA TGATGCAGCA ATATCAGTCA
    CGCCTGGACC AGAGTGAAAA GCGTTTGCGA CAGCAGCAGG TTGAGAAGGA CACGCAGATA
    AAGAGCATCT TAAACAGGTT GATGCTGGTG GAGGAGGAGA TCAGGAAGGA TCACTCACAA
    GAACCAATCG ATATGAAGAG GAGACTTATC GAGGCTCAGG AGAAGAAGTT TCCCTCCGTG
    GGTACAGCAG ACCCGCGTCT GAGGCTGGCG ACTCCATGGA ATGGGGCCCT CCCGCCTCTG
    CCTCCTCGAC TCCAAATCAC AGAAAATGGA GACTTTCTAA ACTCAAGTGA CCATCACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa65729
    Clone ID Related Accession (Same CDS sequence) XM_031892374.1
    Accession Version XM_031892374.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3660bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ras/Rap GTPase-activating protein SynGAP isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCACAATA CAGATGCTCG AGGACCTCCC ATGCATCGTA TGCCCTATGT CACCTCTCCA 
    TACGACCGTC CAGCCTGGAA CCCAAGATAC TGTGTCCTCT CTGGAAATCA GCTTCTCATG
    CTTGATGAGG AGGAGGTTCA CCCGCTGTTG ATACGTGAAA GGAGAACTGA TCCAACCAGA
    ACAAAGTTCC TAAGGCGCAC AGTCAGTGTC CCCGTGGAGG GGAAGCAGCC TGACGAACAC
    GATTATCACC TTGCACGTTC CCGGAGGAAA AGCGTCCCAG GGGGAAAACA GCCCAGTATG
    GACGCCCCTC CCACCGCCCC CTTTCGACCG TCGCAAGGAT TCCTGAGCCG TCGCTTGAAG
    AGCTCTATAA AGAGGACAAA GAGTCAGCCC AAATTAGATA GGACCAGCAG TTTCCGGCAA
    ATATTGCCAA GGTTTCGAAG TGCTGATCAT GATAGGGCAC GGCTCATGCA GGCCTTTAAA
    GAGTCACACT CTCACGAGTC CTTGCTTAGT CCTAGCAGTG CTGCAGAGGC TTTGGATTTG
    AACTTGGATG AGGATTCTGT GGTGAAGCCT GTACACAGCA GCATCTTGGG ACAGGAGTTT
    TGCTTCGAGG TGACCACATC ATCAGGTACC AAATGTTTTG CCTGCCGTTC TGCTGCTGAA
    AGAGACAAAT GGATTGAGAA TCTTCAAAGA GCTGTAAAGC CCAATAAGGA TAACAGCAGG
    CGGGTAGATA ATGTCTTAAA GCTGTGGATT ATTGAAGCCC GTGATCTTCC CCCCAAGAAG
    CGCTATTATT GTGAGCTCTG TTTGGATGAC ATTTTGTATG CCCGTACCAC CAGCAAGCCT
    CGGAATGACA CTGTCTTTTG GGGGGAACAC TTTGAATTTA ATAACCTTCC AGCTGTTCGG
    AACTTGCGCT TGCACCTCTA TAAGGACACA GACAAGAAAA GGCGCAAAGA AAAGAGCAAC
    TATGTGGGCA TGGTGAACGT GCCTATTGCC AGCATTAGTG GGCGCCACTT TGTGGAGCAG
    TGGTACCCGG TTTCTCAGCC CACATCCTAC AAGGGGAAGA GCAGCTGTCC CTCCATTCGC
    CTAAAATCCC GATACCAAAC CATGAGCATC CTCCCCATGG AGCTGTACAA GGAGTTTGCA
    GAGTATGTCA CCAACAACTA CCGGATGCTG TGCGCTGTAC TGGAGCCTGT GCTTAGCGTG
    AAGAGCAAAG AGGAGGTTGC CTGTGCTTTA GTGCATATAC TGCAAAGCAC AGGCAAGGCC
    AAGGACTTCC TTTCAGACAT GGCTATGACC GAGGTTGATC GCTTCCTTGA CAGAGAGCAC
    CTTATTCTCC GTGAGAACAC ACTTGCAACT AAAGCCATAG AGGAGTATCT GAAACTGATA
    GGACAGAGAT ACCTCAAGGA AGCCATTGGT GAGTTTATTC GGGCACTGTA TGAGTCTGAG
    GAGGACTGTG AGGTTGATCC CATGCGTTGT CCACCCTCTG CATTGCCCGA TCACCAGGCC
    AATCTCAGGA TGTGCTGTGA ACTCGCCCTT TGCAAAGTCT TTAACTCTCA TTGTGTGTTT
    CCTCGGGAGT TGAAGGAAGT CTTTGCATCT TGGAGGATGC GCTGTGCAGA GCGCGGCCGG
    GAAGATATAG CAGATCGACT CATCAGTGCC TCTCTCTTCC TCCGATTCCT GTGTCCGGCC
    ATTATGTCCC CCAGTCTCTT CAGCCTAATG CAGGAATACC CTGATGAGCA AACATCTAGG
    ACCCTCACGC TCATCGCAAA AGTCATCCAG AACCTGGCCA ACTTCAACTA CAACAGGTTC
    AGCAGTAAAG AAGAATATAT GTCCTTTATG AATGAGTTCC TAGAGACGGA GTTGGCGAAT
    ATGCAGAATT TCCTGTATGA GATCTCAAAC CTGGATTCGC TCACCAACAG CATTAACTTT
    GAGGGCTACA TTGACCTTGG GCGGGAGCTG AGTACACTTC ATGCCCTGCT TTGGGAAGTG
    ATCCCACAAC TTAGTAAGGA TGCACTTATA AAGTTGGGGC CTCTTCCACG CCTCCTTAAT
    GACATCAGCC TAGCTCTCAG GAACCCCCAC ATCCAACGAC AACCAAGCCA CCAAGAACGA
    CTGCCACCCC CCAAACCTGT CATTCTGAGG GGACCTTCAG CTGAGCTGCA AAGCTACTTG
    GTGCGAGACC TCAACAGCTC TATTGACATG ACTCGCCTAC CCTCACCTAC TAAGGATAAA
    CCATCAAAAG ATATGTTTTT TGTTTCACGT CGCCCGCTAA CTCGCTCCTC GCCAGCCTAT
    TGCACCAGCA GCTCAGACAT CACAGAACCA GACCAGAAGA TGCTGAGCGT GAACAAGAGT
    GTGTCCATGA TGGACTTGCA GGACAGCCGA ATGAATAGCG TAACCAACTT GCAGGCCGTA
    GGTGATCTGC TGAACTCCAG CCAGGCCTCC ATATCAGGGG GCCTACGTCC GGGGGGCCGG
    CTCTCCCAGG GCAGTGGATC CAGCATTGCA GCGGGTCTGA GGCTCAGTCA GATGGGGGTC
    ACCACTGATG GGCTCTCAGC TCAACAGCTA CGCATTCCCT TGTCCTTCCA GAACCCGCTC
    TTCCACATGG CTCCCGATGG GCCTGGTGGG CCCCCATCTC ATCCTGCTCC TCATCGACCA
    GACCCCGGAG CTGATGCCTT TGCCCCTTTC CATGGGTATA GCAAGAGTGA GGACTTGAGC
    TCCGGAAAGC ATATGCTTCA CAGTCACAGC TACAGCGACG AATTCAGCCG GCAGGGGTCA
    GAGTTCAGTA AAAGGCAGTT ATCCCTCCAG GACAATCTCC AGCACATGCT TTCACCCCCT
    CAAATAACCA TTGGCCCTGG AGTGCCACGA AGGCAAGACT CACAGCCTCC CCCTTTGCAG
    AGGGGAAAAT CCCAACAACT GACGGTGCAG GCAGCGCATA AACCTCGGCC AAGCAGTGGC
    AACCTCCTAC AGAGCCCAGA ACCAATGTAT GGGGCAGCAA GAATTAGGCA GCAGTCAGTT
    GAAAAGGATG CAGGTGGCTT GAAACCATCA ATAACCAAGC AGCACTCCCA TGCACAGGCC
    ACTCTGAACC CTGTCACTCC AGCCTCAGAA CGCACAGTTG CCTGGGTATC CAACATGCCC
    CACCTTTCAG CAGATATTGA GAGCGCTCAC ATAGAACGGG AGGAGTATAA GTTGAAGGAA
    TACTCTAAAT CAATGGATGA GAGCCGTCTT GAGAGGGTGA AAGAGTATGA GGAAGAGATT
    TACTCCTTAA AAGAGAGGCT GTTGATGTCA AACCGGAAGC TGGAGGAATA TGAGAGGCGT
    CTTCTTACAC AGGAAGAACA AACTAATAAG ATGATGCAGC AATATCAGTC ACGCCTGGAC
    CAGAGTGAAA AGCGTTTGCG ACAGCAGCAG GTTGAGAAGG ACACGCAGAT AAAGAGCATC
    TTAAACAGGT TAGAAATGGT TGATGCTGGT GGAGGAGGAG ATCAGGAAGG ATCACTCACA
    AGAACCAATC GATATGAAGA GGAGACTTAT CGAGGCTCAG AGAAGAAGTT TCCCTCCGTG
    GGTACAGCAG ACCCGCGTCT GAGGCTGGCG ACTCCATGGA ATGGGGCCCT CCCGCCTCTG
    CCTCCTCGAC TCCAAATCAC AGAAAATGGA GACTTTCTAA ACTCAAGTGA CCATCACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031748234.1
    CDS682..4341
    Translation

    Target ORF information:

    RefSeq Version XM_031892374.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031892374.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCACAATA CAGATGCTCG AGGACCTCCC ATGCATCGTA TGCCCTATGT CACCTCTCCA 
    TACGACCGTC CAGCCTGGAA CCCAAGATAC TGTGTCCTCT CTGGAAATCA GCTTCTCATG
    CTTGATGAGG AGGAGGTTCA CCCGCTGTTG ATACGTGAAA GGAGAACTGA TCCAACCAGA
    ACAAAGTTCC TAAGGCGCAC AGTCAGTGTC CCCGTGGAGG GGAAGCAGCC TGACGAACAC
    GATTATCACC TTGCACGTTC CCGGAGGAAA AGCGTCCCAG GGGGAAAACA GCCCAGTATG
    GACGCCCCTC CCACCGCCCC CTTTCGACCG TCGCAAGGAT TCCTGAGCCG TCGCTTGAAG
    AGCTCTATAA AGAGGACAAA GAGTCAGCCC AAATTAGATA GGACCAGCAG TTTCCGGCAA
    ATATTGCCAA GGTTTCGAAG TGCTGATCAT GATAGGGCAC GGCTCATGCA GGCCTTTAAA
    GAGTCACACT CTCACGAGTC CTTGCTTAGT CCTAGCAGTG CTGCAGAGGC TTTGGATTTG
    AACTTGGATG AGGATTCTGT GGTGAAGCCT GTACACAGCA GCATCTTGGG ACAGGAGTTT
    TGCTTCGAGG TGACCACATC ATCAGGTACC AAATGTTTTG CCTGCCGTTC TGCTGCTGAA
    AGAGACAAAT GGATTGAGAA TCTTCAAAGA GCTGTAAAGC CCAATAAGGA TAACAGCAGG
    CGGGTAGATA ATGTCTTAAA GCTGTGGATT ATTGAAGCCC GTGATCTTCC CCCCAAGAAG
    CGCTATTATT GTGAGCTCTG TTTGGATGAC ATTTTGTATG CCCGTACCAC CAGCAAGCCT
    CGGAATGACA CTGTCTTTTG GGGGGAACAC TTTGAATTTA ATAACCTTCC AGCTGTTCGG
    AACTTGCGCT TGCACCTCTA TAAGGACACA GACAAGAAAA GGCGCAAAGA AAAGAGCAAC
    TATGTGGGCA TGGTGAACGT GCCTATTGCC AGCATTAGTG GGCGCCACTT TGTGGAGCAG
    TGGTACCCGG TTTCTCAGCC CACATCCTAC AAGGGGAAGA GCAGCTGTCC CTCCATTCGC
    CTAAAATCCC GATACCAAAC CATGAGCATC CTCCCCATGG AGCTGTACAA GGAGTTTGCA
    GAGTATGTCA CCAACAACTA CCGGATGCTG TGCGCTGTAC TGGAGCCTGT GCTTAGCGTG
    AAGAGCAAAG AGGAGGTTGC CTGTGCTTTA GTGCATATAC TGCAAAGCAC AGGCAAGGCC
    AAGGACTTCC TTTCAGACAT GGCTATGACC GAGGTTGATC GCTTCCTTGA CAGAGAGCAC
    CTTATTCTCC GTGAGAACAC ACTTGCAACT AAAGCCATAG AGGAGTATCT GAAACTGATA
    GGACAGAGAT ACCTCAAGGA AGCCATTGGT GAGTTTATTC GGGCACTGTA TGAGTCTGAG
    GAGGACTGTG AGGTTGATCC CATGCGTTGT CCACCCTCTG CATTGCCCGA TCACCAGGCC
    AATCTCAGGA TGTGCTGTGA ACTCGCCCTT TGCAAAGTCT TTAACTCTCA TTGTGTGTTT
    CCTCGGGAGT TGAAGGAAGT CTTTGCATCT TGGAGGATGC GCTGTGCAGA GCGCGGCCGG
    GAAGATATAG CAGATCGACT CATCAGTGCC TCTCTCTTCC TCCGATTCCT GTGTCCGGCC
    ATTATGTCCC CCAGTCTCTT CAGCCTAATG CAGGAATACC CTGATGAGCA AACATCTAGG
    ACCCTCACGC TCATCGCAAA AGTCATCCAG AACCTGGCCA ACTTCAACTA CAACAGGTTC
    AGCAGTAAAG AAGAATATAT GTCCTTTATG AATGAGTTCC TAGAGACGGA GTTGGCGAAT
    ATGCAGAATT TCCTGTATGA GATCTCAAAC CTGGATTCGC TCACCAACAG CATTAACTTT
    GAGGGCTACA TTGACCTTGG GCGGGAGCTG AGTACACTTC ATGCCCTGCT TTGGGAAGTG
    ATCCCACAAC TTAGTAAGGA TGCACTTATA AAGTTGGGGC CTCTTCCACG CCTCCTTAAT
    GACATCAGCC TAGCTCTCAG GAACCCCCAC ATCCAACGAC AACCAAGCCA CCAAGAACGA
    CTGCCACCCC CCAAACCTGT CATTCTGAGG GGACCTTCAG CTGAGCTGCA AAGCTACTTG
    GTGCGAGACC TCAACAGCTC TATTGACATG ACTCGCCTAC CCTCACCTAC TAAGGATAAA
    CCATCAAAAG ATATGTTTTT TGTTTCACGT CGCCCGCTAA CTCGCTCCTC GCCAGCCTAT
    TGCACCAGCA GCTCAGACAT CACAGAACCA GACCAGAAGA TGCTGAGCGT GAACAAGAGT
    GTGTCCATGA TGGACTTGCA GGACAGCCGA ATGAATAGCG TAACCAACTT GCAGGCCGTA
    GGTGATCTGC TGAACTCCAG CCAGGCCTCC ATATCAGGGG GCCTACGTCC GGGGGGCCGG
    CTCTCCCAGG GCAGTGGATC CAGCATTGCA GCGGGTCTGA GGCTCAGTCA GATGGGGGTC
    ACCACTGATG GGCTCTCAGC TCAACAGCTA CGCATTCCCT TGTCCTTCCA GAACCCGCTC
    TTCCACATGG CTCCCGATGG GCCTGGTGGG CCCCCATCTC ATCCTGCTCC TCATCGACCA
    GACCCCGGAG CTGATGCCTT TGCCCCTTTC CATGGGTATA GCAAGAGTGA GGACTTGAGC
    TCCGGAAAGC ATATGCTTCA CAGTCACAGC TACAGCGACG AATTCAGCCG GCAGGGGTCA
    GAGTTCAGTA AAAGGCAGTT ATCCCTCCAG GACAATCTCC AGCACATGCT TTCACCCCCT
    CAAATAACCA TTGGCCCTGG AGTGCCACGA AGGCAAGACT CACAGCCTCC CCCTTTGCAG
    AGGGGAAAAT CCCAACAACT GACGGTGCAG GCAGCGCATA AACCTCGGCC AAGCAGTGGC
    AACCTCCTAC AGAGCCCAGA ACCAATGTAT GGGGCAGCAA GAATTAGGCA GCAGTCAGTT
    GAAAAGGATG CAGGTGGCTT GAAACCATCA ATAACCAAGC AGCACTCCCA TGCACAGGCC
    ACTCTGAACC CTGTCACTCC AGCCTCAGAA CGCACAGTTG CCTGGGTATC CAACATGCCC
    CACCTTTCAG CAGATATTGA GAGCGCTCAC ATAGAACGGG AGGAGTATAA GTTGAAGGAA
    TACTCTAAAT CAATGGATGA GAGCCGTCTT GAGAGGGTGA AAGAGTATGA GGAAGAGATT
    TACTCCTTAA AAGAGAGGCT GTTGATGTCA AACCGGAAGC TGGAGGAATA TGAGAGGCGT
    CTTCTTACAC AGGAAGAACA AACTAATAAG ATGATGCAGC AATATCAGTC ACGCCTGGAC
    CAGAGTGAAA AGCGTTTGCG ACAGCAGCAG GTTGAGAAGG ACACGCAGAT AAAGAGCATC
    TTAAACAGGT TAGAAATGGT TGATGCTGGT GGAGGAGGAG ATCAGGAAGG ATCACTCACA
    AGAACCAATC GATATGAAGA GGAGACTTAT CGAGGCTCAG AGAAGAAGTT TCCCTCCGTG
    GGTACAGCAG ACCCGCGTCT GAGGCTGGCG ACTCCATGGA ATGGGGCCCT CCCGCCTCTG
    CCTCCTCGAC TCCAAATCAC AGAAAATGGA GACTTTCTAA ACTCAAGTGA CCATCACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa65730
    Clone ID Related Accession (Same CDS sequence) XM_031892377.1 , XM_031892375.1
    Accession Version XM_031892377.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3630bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ras/Rap GTPase-activating protein SynGAP isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCATCGTA TGCCCTATGT CACCTCTCCA TACGACCGTC CAGCCTGGAA CCCAAGATAC 
    TGTGTCCTCT CTGGAAATCA GCTTCTCATG CTTGATGAGG AGGAGGTTCA CCCGCTGTTG
    ATACGTGAAA GGAGAACTGA TCCAACCAGA ACAAAGTTCC TAAGGCGCAC AGTCAGTGTC
    CCCGTGGAGG GGAAGCAGCC TGACGAACAC GATTATCACC TTGCACGTTC CCGGAGGAAA
    AGCGTCCCAG GGGGAAAACA GCCCAGTATG GACGCCCCTC CCACCGCCCC CTTTCGACCG
    TCGCAAGGAT TCCTGAGCCG TCGCTTGAAG AGCTCTATAA AGAGGACAAA GAGTCAGCCC
    AAATTAGATA GGACCAGCAG TTTCCGGCAA ATATTGCCAA GGTTTCGAAG TGCTGATCAT
    GATAGGGCAC GGCTCATGCA GGCCTTTAAA GAGTCACACT CTCACGAGTC CTTGCTTAGT
    CCTAGCAGTG CTGCAGAGGC TTTGGATTTG AACTTGGATG AGGATTCTGT GGTGAAGCCT
    GTACACAGCA GCATCTTGGG ACAGGAGTTT TGCTTCGAGG TGACCACATC ATCAGGTACC
    AAATGTTTTG CCTGCCGTTC TGCTGCTGAA AGAGACAAAT GGATTGAGAA TCTTCAAAGA
    GCTGTAAAGC CCAATAAGGA TAACAGCAGG CGGGTAGATA ATGTCTTAAA GCTGTGGATT
    ATTGAAGCCC GTGATCTTCC CCCCAAGAAG CGCTATTATT GTGAGCTCTG TTTGGATGAC
    ATTTTGTATG CCCGTACCAC CAGCAAGCCT CGGAATGACA CTGTCTTTTG GGGGGAACAC
    TTTGAATTTA ATAACCTTCC AGCTGTTCGG AACTTGCGCT TGCACCTCTA TAAGGACACA
    GACAAGAAAA GGCGCAAAGA AAAGAGCAAC TATGTGGGCA TGGTGAACGT GCCTATTGCC
    AGCATTAGTG GGCGCCACTT TGTGGAGCAG TGGTACCCGG TTTCTCAGCC CACATCCTAC
    AAGGGGAAGA GCAGCTGTCC CTCCATTCGC CTAAAATCCC GATACCAAAC CATGAGCATC
    CTCCCCATGG AGCTGTACAA GGAGTTTGCA GAGTATGTCA CCAACAACTA CCGGATGCTG
    TGCGCTGTAC TGGAGCCTGT GCTTAGCGTG AAGAGCAAAG AGGAGGTTGC CTGTGCTTTA
    GTGCATATAC TGCAAAGCAC AGGCAAGGCC AAGGACTTCC TTTCAGACAT GGCTATGACC
    GAGGTTGATC GCTTCCTTGA CAGAGAGCAC CTTATTCTCC GTGAGAACAC ACTTGCAACT
    AAAGCCATAG AGGAGTATCT GAAACTGATA GGACAGAGAT ACCTCAAGGA AGCCATTGGT
    GAGTTTATTC GGGCACTGTA TGAGTCTGAG GAGGACTGTG AGGTTGATCC CATGCGTTGT
    CCACCCTCTG CATTGCCCGA TCACCAGGCC AATCTCAGGA TGTGCTGTGA ACTCGCCCTT
    TGCAAAGTCT TTAACTCTCA TTGTGTGTTT CCTCGGGAGT TGAAGGAAGT CTTTGCATCT
    TGGAGGATGC GCTGTGCAGA GCGCGGCCGG GAAGATATAG CAGATCGACT CATCAGTGCC
    TCTCTCTTCC TCCGATTCCT GTGTCCGGCC ATTATGTCCC CCAGTCTCTT CAGCCTAATG
    CAGGAATACC CTGATGAGCA AACATCTAGG ACCCTCACGC TCATCGCAAA AGTCATCCAG
    AACCTGGCCA ACTTCAACTA CAACAGGTTC AGCAGTAAAG AAGAATATAT GTCCTTTATG
    AATGAGTTCC TAGAGACGGA GTTGGCGAAT ATGCAGAATT TCCTGTATGA GATCTCAAAC
    CTGGATTCGC TCACCAACAG CATTAACTTT GAGGGCTACA TTGACCTTGG GCGGGAGCTG
    AGTACACTTC ATGCCCTGCT TTGGGAAGTG ATCCCACAAC TTAGTAAGGA TGCACTTATA
    AAGTTGGGGC CTCTTCCACG CCTCCTTAAT GACATCAGCC TAGCTCTCAG GAACCCCCAC
    ATCCAACGAC AACCAAGCCA CCAAGAACGA CTGCCACCCC CCAAACCTGT CATTCTGAGG
    GGACCTTCAG CTGAGCTGCA AAGCTACTTG GTGCGAGACC TCAACAGCTC TATTGACATG
    ACTCGCCTAC CCTCACCTAC TAAGGATAAA CCATCAAAAG ATATGTTTTT TGTTTCACGT
    CGCCCGCTAA CTCGCTCCTC GCCAGCCTAT TGCACCAGCA GCTCAGACAT CACAGAACCA
    GACCAGAAGA TGCTGAGCGT GAACAAGAGT GTGTCCATGA TGGACTTGCA GGACAGCCGA
    ATGAATAGCG TAACCAACTT GCAGGCCGTA GGTGATCTGC TGAACTCCAG CCAGGCCTCC
    ATATCAGGGG GCCTACGTCC GGGGGGCCGG CTCTCCCAGG GCAGTGGATC CAGCATTGCA
    GCGGGTCTGA GGCTCAGTCA GATGGGGGTC ACCACTGATG GGCTCTCAGC TCAACAGCTA
    CGCATTCCCT TGTCCTTCCA GAACCCGCTC TTCCACATGG CTCCCGATGG GCCTGGTGGG
    CCCCCATCTC ATCCTGCTCC TCATCGACCA GACCCCGGAG CTGATGCCTT TGCCCCTTTC
    CATGGGTATA GCAAGAGTGA GGACTTGAGC TCCGGAAAGC ATATGCTTCA CAGTCACAGC
    TACAGCGACG AATTCAGCCG GCAGGGGTCA GAGTTCAGTA AAAGGCAGTT ATCCCTCCAG
    GACAATCTCC AGCACATGCT TTCACCCCCT CAAATAACCA TTGGCCCTGG AGTGCCACGA
    AGGCAAGACT CACAGCCTCC CCCTTTGCAG AGGGGAAAAT CCCAACAACT GACGGTGCAG
    GCAGCGCATA AACCTCGGCC AAGCAGTGGC AACCTCCTAC AGAGCCCAGA ACCAATGTAT
    GGGGCAGCAA GAATTAGGCA GCAGTCAGTT GAAAAGGATG CAGGTGGCTT GAAACCATCA
    ATAACCAAGC AGCACTCCCA TGCACAGGCC ACTCTGAACC CTGTCACTCC AGCCTCAGAA
    CGCACAGTTG CCTGGGTATC CAACATGCCC CACCTTTCAG CAGATATTGA GAGCGCTCAC
    ATAGAACGGG AGGAGTATAA GTTGAAGGAA TACTCTAAAT CAATGGATGA GAGCCGTCTT
    GAGAGGGTGA AAGAGTATGA GGAAGAGATT TACTCCTTAA AAGAGAGGCT GTTGATGTCA
    AACCGGAAGC TGGAGGAATA TGAGAGGCGT CTTCTTACAC AGGAAGAACA AACTAATAAG
    ATGATGCAGC AATATCAGTC ACGCCTGGAC CAGAGTGAAA AGCGTTTGCG ACAGCAGCAG
    GTTGAGAAGG ACACGCAGAT AAAGAGCATC TTAAACAGGT TAGAAATGGT TGATGCTGGT
    GGAGGAGGAG ATCAGGAAGG ATCACTCACA AGAACCAATC GATATGAAGA GGAGACTTAT
    CGAGGCTCAG AGAAGAAGTT TCCCTCCGTG GGTACAGCAG ACCCGCGTCT GAGGCTGGCG
    ACTCCATGGA ATGGGGCCCT CCCGCCTCTG CCTCCTCGAC TCCAAATCAC AGAAAATGGA
    GACTTTCTAA ACTCAAGTGA CCATCACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031748237.1
    CDS50..3679
    Translation

    Target ORF information:

    RefSeq Version XM_031892377.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031892377.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCATCGTA TGCCCTATGT CACCTCTCCA TACGACCGTC CAGCCTGGAA CCCAAGATAC 
    TGTGTCCTCT CTGGAAATCA GCTTCTCATG CTTGATGAGG AGGAGGTTCA CCCGCTGTTG
    ATACGTGAAA GGAGAACTGA TCCAACCAGA ACAAAGTTCC TAAGGCGCAC AGTCAGTGTC
    CCCGTGGAGG GGAAGCAGCC TGACGAACAC GATTATCACC TTGCACGTTC CCGGAGGAAA
    AGCGTCCCAG GGGGAAAACA GCCCAGTATG GACGCCCCTC CCACCGCCCC CTTTCGACCG
    TCGCAAGGAT TCCTGAGCCG TCGCTTGAAG AGCTCTATAA AGAGGACAAA GAGTCAGCCC
    AAATTAGATA GGACCAGCAG TTTCCGGCAA ATATTGCCAA GGTTTCGAAG TGCTGATCAT
    GATAGGGCAC GGCTCATGCA GGCCTTTAAA GAGTCACACT CTCACGAGTC CTTGCTTAGT
    CCTAGCAGTG CTGCAGAGGC TTTGGATTTG AACTTGGATG AGGATTCTGT GGTGAAGCCT
    GTACACAGCA GCATCTTGGG ACAGGAGTTT TGCTTCGAGG TGACCACATC ATCAGGTACC
    AAATGTTTTG CCTGCCGTTC TGCTGCTGAA AGAGACAAAT GGATTGAGAA TCTTCAAAGA
    GCTGTAAAGC CCAATAAGGA TAACAGCAGG CGGGTAGATA ATGTCTTAAA GCTGTGGATT
    ATTGAAGCCC GTGATCTTCC CCCCAAGAAG CGCTATTATT GTGAGCTCTG TTTGGATGAC
    ATTTTGTATG CCCGTACCAC CAGCAAGCCT CGGAATGACA CTGTCTTTTG GGGGGAACAC
    TTTGAATTTA ATAACCTTCC AGCTGTTCGG AACTTGCGCT TGCACCTCTA TAAGGACACA
    GACAAGAAAA GGCGCAAAGA AAAGAGCAAC TATGTGGGCA TGGTGAACGT GCCTATTGCC
    AGCATTAGTG GGCGCCACTT TGTGGAGCAG TGGTACCCGG TTTCTCAGCC CACATCCTAC
    AAGGGGAAGA GCAGCTGTCC CTCCATTCGC CTAAAATCCC GATACCAAAC CATGAGCATC
    CTCCCCATGG AGCTGTACAA GGAGTTTGCA GAGTATGTCA CCAACAACTA CCGGATGCTG
    TGCGCTGTAC TGGAGCCTGT GCTTAGCGTG AAGAGCAAAG AGGAGGTTGC CTGTGCTTTA
    GTGCATATAC TGCAAAGCAC AGGCAAGGCC AAGGACTTCC TTTCAGACAT GGCTATGACC
    GAGGTTGATC GCTTCCTTGA CAGAGAGCAC CTTATTCTCC GTGAGAACAC ACTTGCAACT
    AAAGCCATAG AGGAGTATCT GAAACTGATA GGACAGAGAT ACCTCAAGGA AGCCATTGGT
    GAGTTTATTC GGGCACTGTA TGAGTCTGAG GAGGACTGTG AGGTTGATCC CATGCGTTGT
    CCACCCTCTG CATTGCCCGA TCACCAGGCC AATCTCAGGA TGTGCTGTGA ACTCGCCCTT
    TGCAAAGTCT TTAACTCTCA TTGTGTGTTT CCTCGGGAGT TGAAGGAAGT CTTTGCATCT
    TGGAGGATGC GCTGTGCAGA GCGCGGCCGG GAAGATATAG CAGATCGACT CATCAGTGCC
    TCTCTCTTCC TCCGATTCCT GTGTCCGGCC ATTATGTCCC CCAGTCTCTT CAGCCTAATG
    CAGGAATACC CTGATGAGCA AACATCTAGG ACCCTCACGC TCATCGCAAA AGTCATCCAG
    AACCTGGCCA ACTTCAACTA CAACAGGTTC AGCAGTAAAG AAGAATATAT GTCCTTTATG
    AATGAGTTCC TAGAGACGGA GTTGGCGAAT ATGCAGAATT TCCTGTATGA GATCTCAAAC
    CTGGATTCGC TCACCAACAG CATTAACTTT GAGGGCTACA TTGACCTTGG GCGGGAGCTG
    AGTACACTTC ATGCCCTGCT TTGGGAAGTG ATCCCACAAC TTAGTAAGGA TGCACTTATA
    AAGTTGGGGC CTCTTCCACG CCTCCTTAAT GACATCAGCC TAGCTCTCAG GAACCCCCAC
    ATCCAACGAC AACCAAGCCA CCAAGAACGA CTGCCACCCC CCAAACCTGT CATTCTGAGG
    GGACCTTCAG CTGAGCTGCA AAGCTACTTG GTGCGAGACC TCAACAGCTC TATTGACATG
    ACTCGCCTAC CCTCACCTAC TAAGGATAAA CCATCAAAAG ATATGTTTTT TGTTTCACGT
    CGCCCGCTAA CTCGCTCCTC GCCAGCCTAT TGCACCAGCA GCTCAGACAT CACAGAACCA
    GACCAGAAGA TGCTGAGCGT GAACAAGAGT GTGTCCATGA TGGACTTGCA GGACAGCCGA
    ATGAATAGCG TAACCAACTT GCAGGCCGTA GGTGATCTGC TGAACTCCAG CCAGGCCTCC
    ATATCAGGGG GCCTACGTCC GGGGGGCCGG CTCTCCCAGG GCAGTGGATC CAGCATTGCA
    GCGGGTCTGA GGCTCAGTCA GATGGGGGTC ACCACTGATG GGCTCTCAGC TCAACAGCTA
    CGCATTCCCT TGTCCTTCCA GAACCCGCTC TTCCACATGG CTCCCGATGG GCCTGGTGGG
    CCCCCATCTC ATCCTGCTCC TCATCGACCA GACCCCGGAG CTGATGCCTT TGCCCCTTTC
    CATGGGTATA GCAAGAGTGA GGACTTGAGC TCCGGAAAGC ATATGCTTCA CAGTCACAGC
    TACAGCGACG AATTCAGCCG GCAGGGGTCA GAGTTCAGTA AAAGGCAGTT ATCCCTCCAG
    GACAATCTCC AGCACATGCT TTCACCCCCT CAAATAACCA TTGGCCCTGG AGTGCCACGA
    AGGCAAGACT CACAGCCTCC CCCTTTGCAG AGGGGAAAAT CCCAACAACT GACGGTGCAG
    GCAGCGCATA AACCTCGGCC AAGCAGTGGC AACCTCCTAC AGAGCCCAGA ACCAATGTAT
    GGGGCAGCAA GAATTAGGCA GCAGTCAGTT GAAAAGGATG CAGGTGGCTT GAAACCATCA
    ATAACCAAGC AGCACTCCCA TGCACAGGCC ACTCTGAACC CTGTCACTCC AGCCTCAGAA
    CGCACAGTTG CCTGGGTATC CAACATGCCC CACCTTTCAG CAGATATTGA GAGCGCTCAC
    ATAGAACGGG AGGAGTATAA GTTGAAGGAA TACTCTAAAT CAATGGATGA GAGCCGTCTT
    GAGAGGGTGA AAGAGTATGA GGAAGAGATT TACTCCTTAA AAGAGAGGCT GTTGATGTCA
    AACCGGAAGC TGGAGGAATA TGAGAGGCGT CTTCTTACAC AGGAAGAACA AACTAATAAG
    ATGATGCAGC AATATCAGTC ACGCCTGGAC CAGAGTGAAA AGCGTTTGCG ACAGCAGCAG
    GTTGAGAAGG ACACGCAGAT AAAGAGCATC TTAAACAGGT TAGAAATGGT TGATGCTGGT
    GGAGGAGGAG ATCAGGAAGG ATCACTCACA AGAACCAATC GATATGAAGA GGAGACTTAT
    CGAGGCTCAG AGAAGAAGTT TCCCTCCGTG GGTACAGCAG ACCCGCGTCT GAGGCTGGCG
    ACTCCATGGA ATGGGGCCCT CCCGCCTCTG CCTCCTCGAC TCCAAATCAC AGAAAATGGA
    GACTTTCTAA ACTCAAGTGA CCATCACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa65730
    Clone ID Related Accession (Same CDS sequence) XM_031892377.1 , XM_031892375.1
    Accession Version XM_031892375.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3630bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ras/Rap GTPase-activating protein SynGAP isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCATCGTA TGCCCTATGT CACCTCTCCA TACGACCGTC CAGCCTGGAA CCCAAGATAC 
    TGTGTCCTCT CTGGAAATCA GCTTCTCATG CTTGATGAGG AGGAGGTTCA CCCGCTGTTG
    ATACGTGAAA GGAGAACTGA TCCAACCAGA ACAAAGTTCC TAAGGCGCAC AGTCAGTGTC
    CCCGTGGAGG GGAAGCAGCC TGACGAACAC GATTATCACC TTGCACGTTC CCGGAGGAAA
    AGCGTCCCAG GGGGAAAACA GCCCAGTATG GACGCCCCTC CCACCGCCCC CTTTCGACCG
    TCGCAAGGAT TCCTGAGCCG TCGCTTGAAG AGCTCTATAA AGAGGACAAA GAGTCAGCCC
    AAATTAGATA GGACCAGCAG TTTCCGGCAA ATATTGCCAA GGTTTCGAAG TGCTGATCAT
    GATAGGGCAC GGCTCATGCA GGCCTTTAAA GAGTCACACT CTCACGAGTC CTTGCTTAGT
    CCTAGCAGTG CTGCAGAGGC TTTGGATTTG AACTTGGATG AGGATTCTGT GGTGAAGCCT
    GTACACAGCA GCATCTTGGG ACAGGAGTTT TGCTTCGAGG TGACCACATC ATCAGGTACC
    AAATGTTTTG CCTGCCGTTC TGCTGCTGAA AGAGACAAAT GGATTGAGAA TCTTCAAAGA
    GCTGTAAAGC CCAATAAGGA TAACAGCAGG CGGGTAGATA ATGTCTTAAA GCTGTGGATT
    ATTGAAGCCC GTGATCTTCC CCCCAAGAAG CGCTATTATT GTGAGCTCTG TTTGGATGAC
    ATTTTGTATG CCCGTACCAC CAGCAAGCCT CGGAATGACA CTGTCTTTTG GGGGGAACAC
    TTTGAATTTA ATAACCTTCC AGCTGTTCGG AACTTGCGCT TGCACCTCTA TAAGGACACA
    GACAAGAAAA GGCGCAAAGA AAAGAGCAAC TATGTGGGCA TGGTGAACGT GCCTATTGCC
    AGCATTAGTG GGCGCCACTT TGTGGAGCAG TGGTACCCGG TTTCTCAGCC CACATCCTAC
    AAGGGGAAGA GCAGCTGTCC CTCCATTCGC CTAAAATCCC GATACCAAAC CATGAGCATC
    CTCCCCATGG AGCTGTACAA GGAGTTTGCA GAGTATGTCA CCAACAACTA CCGGATGCTG
    TGCGCTGTAC TGGAGCCTGT GCTTAGCGTG AAGAGCAAAG AGGAGGTTGC CTGTGCTTTA
    GTGCATATAC TGCAAAGCAC AGGCAAGGCC AAGGACTTCC TTTCAGACAT GGCTATGACC
    GAGGTTGATC GCTTCCTTGA CAGAGAGCAC CTTATTCTCC GTGAGAACAC ACTTGCAACT
    AAAGCCATAG AGGAGTATCT GAAACTGATA GGACAGAGAT ACCTCAAGGA AGCCATTGGT
    GAGTTTATTC GGGCACTGTA TGAGTCTGAG GAGGACTGTG AGGTTGATCC CATGCGTTGT
    CCACCCTCTG CATTGCCCGA TCACCAGGCC AATCTCAGGA TGTGCTGTGA ACTCGCCCTT
    TGCAAAGTCT TTAACTCTCA TTGTGTGTTT CCTCGGGAGT TGAAGGAAGT CTTTGCATCT
    TGGAGGATGC GCTGTGCAGA GCGCGGCCGG GAAGATATAG CAGATCGACT CATCAGTGCC
    TCTCTCTTCC TCCGATTCCT GTGTCCGGCC ATTATGTCCC CCAGTCTCTT CAGCCTAATG
    CAGGAATACC CTGATGAGCA AACATCTAGG ACCCTCACGC TCATCGCAAA AGTCATCCAG
    AACCTGGCCA ACTTCAACTA CAACAGGTTC AGCAGTAAAG AAGAATATAT GTCCTTTATG
    AATGAGTTCC TAGAGACGGA GTTGGCGAAT ATGCAGAATT TCCTGTATGA GATCTCAAAC
    CTGGATTCGC TCACCAACAG CATTAACTTT GAGGGCTACA TTGACCTTGG GCGGGAGCTG
    AGTACACTTC ATGCCCTGCT TTGGGAAGTG ATCCCACAAC TTAGTAAGGA TGCACTTATA
    AAGTTGGGGC CTCTTCCACG CCTCCTTAAT GACATCAGCC TAGCTCTCAG GAACCCCCAC
    ATCCAACGAC AACCAAGCCA CCAAGAACGA CTGCCACCCC CCAAACCTGT CATTCTGAGG
    GGACCTTCAG CTGAGCTGCA AAGCTACTTG GTGCGAGACC TCAACAGCTC TATTGACATG
    ACTCGCCTAC CCTCACCTAC TAAGGATAAA CCATCAAAAG ATATGTTTTT TGTTTCACGT
    CGCCCGCTAA CTCGCTCCTC GCCAGCCTAT TGCACCAGCA GCTCAGACAT CACAGAACCA
    GACCAGAAGA TGCTGAGCGT GAACAAGAGT GTGTCCATGA TGGACTTGCA GGACAGCCGA
    ATGAATAGCG TAACCAACTT GCAGGCCGTA GGTGATCTGC TGAACTCCAG CCAGGCCTCC
    ATATCAGGGG GCCTACGTCC GGGGGGCCGG CTCTCCCAGG GCAGTGGATC CAGCATTGCA
    GCGGGTCTGA GGCTCAGTCA GATGGGGGTC ACCACTGATG GGCTCTCAGC TCAACAGCTA
    CGCATTCCCT TGTCCTTCCA GAACCCGCTC TTCCACATGG CTCCCGATGG GCCTGGTGGG
    CCCCCATCTC ATCCTGCTCC TCATCGACCA GACCCCGGAG CTGATGCCTT TGCCCCTTTC
    CATGGGTATA GCAAGAGTGA GGACTTGAGC TCCGGAAAGC ATATGCTTCA CAGTCACAGC
    TACAGCGACG AATTCAGCCG GCAGGGGTCA GAGTTCAGTA AAAGGCAGTT ATCCCTCCAG
    GACAATCTCC AGCACATGCT TTCACCCCCT CAAATAACCA TTGGCCCTGG AGTGCCACGA
    AGGCAAGACT CACAGCCTCC CCCTTTGCAG AGGGGAAAAT CCCAACAACT GACGGTGCAG
    GCAGCGCATA AACCTCGGCC AAGCAGTGGC AACCTCCTAC AGAGCCCAGA ACCAATGTAT
    GGGGCAGCAA GAATTAGGCA GCAGTCAGTT GAAAAGGATG CAGGTGGCTT GAAACCATCA
    ATAACCAAGC AGCACTCCCA TGCACAGGCC ACTCTGAACC CTGTCACTCC AGCCTCAGAA
    CGCACAGTTG CCTGGGTATC CAACATGCCC CACCTTTCAG CAGATATTGA GAGCGCTCAC
    ATAGAACGGG AGGAGTATAA GTTGAAGGAA TACTCTAAAT CAATGGATGA GAGCCGTCTT
    GAGAGGGTGA AAGAGTATGA GGAAGAGATT TACTCCTTAA AAGAGAGGCT GTTGATGTCA
    AACCGGAAGC TGGAGGAATA TGAGAGGCGT CTTCTTACAC AGGAAGAACA AACTAATAAG
    ATGATGCAGC AATATCAGTC ACGCCTGGAC CAGAGTGAAA AGCGTTTGCG ACAGCAGCAG
    GTTGAGAAGG ACACGCAGAT AAAGAGCATC TTAAACAGGT TAGAAATGGT TGATGCTGGT
    GGAGGAGGAG ATCAGGAAGG ATCACTCACA AGAACCAATC GATATGAAGA GGAGACTTAT
    CGAGGCTCAG AGAAGAAGTT TCCCTCCGTG GGTACAGCAG ACCCGCGTCT GAGGCTGGCG
    ACTCCATGGA ATGGGGCCCT CCCGCCTCTG CCTCCTCGAC TCCAAATCAC AGAAAATGGA
    GACTTTCTAA ACTCAAGTGA CCATCACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031748235.1
    CDS78..3707
    Translation

    Target ORF information:

    RefSeq Version XM_031892375.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031892375.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCATCGTA TGCCCTATGT CACCTCTCCA TACGACCGTC CAGCCTGGAA CCCAAGATAC 
    TGTGTCCTCT CTGGAAATCA GCTTCTCATG CTTGATGAGG AGGAGGTTCA CCCGCTGTTG
    ATACGTGAAA GGAGAACTGA TCCAACCAGA ACAAAGTTCC TAAGGCGCAC AGTCAGTGTC
    CCCGTGGAGG GGAAGCAGCC TGACGAACAC GATTATCACC TTGCACGTTC CCGGAGGAAA
    AGCGTCCCAG GGGGAAAACA GCCCAGTATG GACGCCCCTC CCACCGCCCC CTTTCGACCG
    TCGCAAGGAT TCCTGAGCCG TCGCTTGAAG AGCTCTATAA AGAGGACAAA GAGTCAGCCC
    AAATTAGATA GGACCAGCAG TTTCCGGCAA ATATTGCCAA GGTTTCGAAG TGCTGATCAT
    GATAGGGCAC GGCTCATGCA GGCCTTTAAA GAGTCACACT CTCACGAGTC CTTGCTTAGT
    CCTAGCAGTG CTGCAGAGGC TTTGGATTTG AACTTGGATG AGGATTCTGT GGTGAAGCCT
    GTACACAGCA GCATCTTGGG ACAGGAGTTT TGCTTCGAGG TGACCACATC ATCAGGTACC
    AAATGTTTTG CCTGCCGTTC TGCTGCTGAA AGAGACAAAT GGATTGAGAA TCTTCAAAGA
    GCTGTAAAGC CCAATAAGGA TAACAGCAGG CGGGTAGATA ATGTCTTAAA GCTGTGGATT
    ATTGAAGCCC GTGATCTTCC CCCCAAGAAG CGCTATTATT GTGAGCTCTG TTTGGATGAC
    ATTTTGTATG CCCGTACCAC CAGCAAGCCT CGGAATGACA CTGTCTTTTG GGGGGAACAC
    TTTGAATTTA ATAACCTTCC AGCTGTTCGG AACTTGCGCT TGCACCTCTA TAAGGACACA
    GACAAGAAAA GGCGCAAAGA AAAGAGCAAC TATGTGGGCA TGGTGAACGT GCCTATTGCC
    AGCATTAGTG GGCGCCACTT TGTGGAGCAG TGGTACCCGG TTTCTCAGCC CACATCCTAC
    AAGGGGAAGA GCAGCTGTCC CTCCATTCGC CTAAAATCCC GATACCAAAC CATGAGCATC
    CTCCCCATGG AGCTGTACAA GGAGTTTGCA GAGTATGTCA CCAACAACTA CCGGATGCTG
    TGCGCTGTAC TGGAGCCTGT GCTTAGCGTG AAGAGCAAAG AGGAGGTTGC CTGTGCTTTA
    GTGCATATAC TGCAAAGCAC AGGCAAGGCC AAGGACTTCC TTTCAGACAT GGCTATGACC
    GAGGTTGATC GCTTCCTTGA CAGAGAGCAC CTTATTCTCC GTGAGAACAC ACTTGCAACT
    AAAGCCATAG AGGAGTATCT GAAACTGATA GGACAGAGAT ACCTCAAGGA AGCCATTGGT
    GAGTTTATTC GGGCACTGTA TGAGTCTGAG GAGGACTGTG AGGTTGATCC CATGCGTTGT
    CCACCCTCTG CATTGCCCGA TCACCAGGCC AATCTCAGGA TGTGCTGTGA ACTCGCCCTT
    TGCAAAGTCT TTAACTCTCA TTGTGTGTTT CCTCGGGAGT TGAAGGAAGT CTTTGCATCT
    TGGAGGATGC GCTGTGCAGA GCGCGGCCGG GAAGATATAG CAGATCGACT CATCAGTGCC
    TCTCTCTTCC TCCGATTCCT GTGTCCGGCC ATTATGTCCC CCAGTCTCTT CAGCCTAATG
    CAGGAATACC CTGATGAGCA AACATCTAGG ACCCTCACGC TCATCGCAAA AGTCATCCAG
    AACCTGGCCA ACTTCAACTA CAACAGGTTC AGCAGTAAAG AAGAATATAT GTCCTTTATG
    AATGAGTTCC TAGAGACGGA GTTGGCGAAT ATGCAGAATT TCCTGTATGA GATCTCAAAC
    CTGGATTCGC TCACCAACAG CATTAACTTT GAGGGCTACA TTGACCTTGG GCGGGAGCTG
    AGTACACTTC ATGCCCTGCT TTGGGAAGTG ATCCCACAAC TTAGTAAGGA TGCACTTATA
    AAGTTGGGGC CTCTTCCACG CCTCCTTAAT GACATCAGCC TAGCTCTCAG GAACCCCCAC
    ATCCAACGAC AACCAAGCCA CCAAGAACGA CTGCCACCCC CCAAACCTGT CATTCTGAGG
    GGACCTTCAG CTGAGCTGCA AAGCTACTTG GTGCGAGACC TCAACAGCTC TATTGACATG
    ACTCGCCTAC CCTCACCTAC TAAGGATAAA CCATCAAAAG ATATGTTTTT TGTTTCACGT
    CGCCCGCTAA CTCGCTCCTC GCCAGCCTAT TGCACCAGCA GCTCAGACAT CACAGAACCA
    GACCAGAAGA TGCTGAGCGT GAACAAGAGT GTGTCCATGA TGGACTTGCA GGACAGCCGA
    ATGAATAGCG TAACCAACTT GCAGGCCGTA GGTGATCTGC TGAACTCCAG CCAGGCCTCC
    ATATCAGGGG GCCTACGTCC GGGGGGCCGG CTCTCCCAGG GCAGTGGATC CAGCATTGCA
    GCGGGTCTGA GGCTCAGTCA GATGGGGGTC ACCACTGATG GGCTCTCAGC TCAACAGCTA
    CGCATTCCCT TGTCCTTCCA GAACCCGCTC TTCCACATGG CTCCCGATGG GCCTGGTGGG
    CCCCCATCTC ATCCTGCTCC TCATCGACCA GACCCCGGAG CTGATGCCTT TGCCCCTTTC
    CATGGGTATA GCAAGAGTGA GGACTTGAGC TCCGGAAAGC ATATGCTTCA CAGTCACAGC
    TACAGCGACG AATTCAGCCG GCAGGGGTCA GAGTTCAGTA AAAGGCAGTT ATCCCTCCAG
    GACAATCTCC AGCACATGCT TTCACCCCCT CAAATAACCA TTGGCCCTGG AGTGCCACGA
    AGGCAAGACT CACAGCCTCC CCCTTTGCAG AGGGGAAAAT CCCAACAACT GACGGTGCAG
    GCAGCGCATA AACCTCGGCC AAGCAGTGGC AACCTCCTAC AGAGCCCAGA ACCAATGTAT
    GGGGCAGCAA GAATTAGGCA GCAGTCAGTT GAAAAGGATG CAGGTGGCTT GAAACCATCA
    ATAACCAAGC AGCACTCCCA TGCACAGGCC ACTCTGAACC CTGTCACTCC AGCCTCAGAA
    CGCACAGTTG CCTGGGTATC CAACATGCCC CACCTTTCAG CAGATATTGA GAGCGCTCAC
    ATAGAACGGG AGGAGTATAA GTTGAAGGAA TACTCTAAAT CAATGGATGA GAGCCGTCTT
    GAGAGGGTGA AAGAGTATGA GGAAGAGATT TACTCCTTAA AAGAGAGGCT GTTGATGTCA
    AACCGGAAGC TGGAGGAATA TGAGAGGCGT CTTCTTACAC AGGAAGAACA AACTAATAAG
    ATGATGCAGC AATATCAGTC ACGCCTGGAC CAGAGTGAAA AGCGTTTGCG ACAGCAGCAG
    GTTGAGAAGG ACACGCAGAT AAAGAGCATC TTAAACAGGT TAGAAATGGT TGATGCTGGT
    GGAGGAGGAG ATCAGGAAGG ATCACTCACA AGAACCAATC GATATGAAGA GGAGACTTAT
    CGAGGCTCAG AGAAGAAGTT TCCCTCCGTG GGTACAGCAG ACCCGCGTCT GAGGCTGGCG
    ACTCCATGGA ATGGGGCCCT CCCGCCTCTG CCTCCTCGAC TCCAAATCAC AGAAAATGGA
    GACTTTCTAA ACTCAAGTGA CCATCACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa65731
    Clone ID Related Accession (Same CDS sequence) XM_031892378.1
    Accession Version XM_031892378.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3546bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ras/Rap GTPase-activating protein SynGAP isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGATGTTCT ATGCTCCCTT CAGAGATGCT CGAGGACCTC CCATGCATCG TATGCCCTAT 
    GTCACCTCTC CATACGACCG TCCAGCCTGG AACCCAAGAT ACTGTGTCCT CTCTGGAAAT
    CAGCTTCTCA TGCTTGATGA GGAGGAGGTT CACCCGCTGT TGATACGTGA AAGGAGAACT
    GATCCAACCA GAACAAAGTT CCTAAGGCGC ACAGTCAGTG TCCCCGTGGA GGGGAAGCAG
    CCTGACGAAC ACGATTATCA CCTTGCACGT TCCCGGAGGA AAAGCGTCCC AGGGGGAAAA
    CAGCCCAGTA TGGACGCCCC TCCCACCGCC CCCTTTCGAC CGTCGCAAGG ATTCCTGAGC
    CGTCGCTTGA AGAGCTCTAT AAAGAGGACA AAGAGTCAGC CCAAATTAGA TAGGACCAGC
    AGTTTCCGGC AAATATTGCC AAGGTTTCGA AGTGCTGATC ATGATAGGGC ACGGCTCATG
    CAGGCCTTTA AAGAGTCACA CTCTCACGAG TCCTTGCTTA GTCCTAGCAG TGCTGCAGAG
    GCTTTGGATT TGAACTTGGA TGAGGATTCT GTGGTGAAGC CTGTACACAG CAGCATCTTG
    GGACAGGAGT TTTGCTTCGA GGTGACCACA TCATCAGGTA CCAAATGTTT TGCCTGCCGT
    TCTGCTGCTG AAAGAGACAA ATGGATTGAG AATCTTCAAA GAGCTGTAAA GCCCAATAAG
    GATAACAGCA GGCGGGTAGA TAATGTCTTA AAGCTGTGGA TTATTGAAGC CCGTGATCTT
    CCCCCCAAGA AGCGCTATTA TTGTGAGCTC TGTTTGGATG ACATTTTGTA TGCCCGTACC
    ACCAGCAAGC CTCGGAATGA CACTGTCTTT TGGGGGGAAC ACTTTGAATT TAATAACCTT
    CCAGCTGTTC GGAACTTGCG CTTGCACCTC TATAAGGACA CAGACAAGAA AAGGCGCAAA
    GAAAAGAGCA ACTATGTGGG CATGGTGAAC GTGCCTATTG CCAGCATTAG TGGGCGCCAC
    TTTGTGGAGC AGTGGTACCC GGTTTCTCAG CCCACATCCT ACAAGGGGAA GAGCAGCTGT
    CCCTCCATTC GCCTAAAATC CCGATACCAA ACCATGAGCA TCCTCCCCAT GGAGCTGTAC
    AAGGAGTTTG CAGAGTATGT CACCAACAAC TACCGGATGC TGTGCGCTGT ACTGGAGCCT
    GTGCTTAGCG TGAAGAGCAA AGAGGAGGTT GCCTGTGCTT TAGTGCATAT ACTGCAAAGC
    ACAGGCAAGG CCAAGGACTT CCTTTCAGAC ATGGCTATGA CCGAGGTTGA TCGCTTCCTT
    GACAGAGAGC ACCTTATTCT CCGTGAGAAC ACACTTGCAA CTAAAGCCAT AGAGGAGTAT
    CTGAAACTGA TAGGACAGAG ATACCTCAAG GAAGCCATTG GTGAGTTTAT TCGGGCACTG
    TATGAGTCTG AGGAGGACTG TGAGGTTGAT CCCATGCGTT GTCCACCCTC TGCATTGCCC
    GATCACCAGG CCAATCTCAG GATGTGCTGT GAACTCGCCC TTTGCAAAGT CTTTAACTCT
    CATTGTGTGT TTCCTCGGGA GTTGAAGGAA GTCTTTGCAT CTTGGAGGAT GCGCTGTGCA
    GAGCGCGGCC GGGAAGATAT AGCAGATCGA CTCATCAGTG CCTCTCTCTT CCTCCGATTC
    CTGTGTCCGG CCATTATGTC CCCCAGTCTC TTCAGCCTAA TGCAGGAATA CCCTGATGAG
    CAAACATCTA GGACCCTCAC GCTCATCGCA AAAGTCATCC AGAACCTGGC CAACTTCAAC
    TACAACAGGT TCAGCAGTAA AGAAGAATAT ATGTCCTTTA TGAATGAGTT CCTAGAGACG
    GAGTTGGCGA ATATGCAGAA TTTCCTGTAT GAGATCTCAA ACCTGGATTC GCTCACCAAC
    AGCATTAACT TTGAGGGCTA CATTGACCTT GGGCGGGAGC TGAGTACACT TCATGCCCTG
    CTTTGGGAAG TGATCCCACA ACTTAGTAAG GATGCACTTA TAAAGTTGGG GCCTCTTCCA
    CGCCTCCTTA ATGACATCAG CCTAGCTCTC AGGAACCCCC ACATCCAACG ACAACCAAGC
    CACCAAGAAC GACTGCCACC CCCCAAACCT GTCATTCTGA GGGGACCTTC AGCTGAGCTG
    CAAAGCTACT TGGTGCGAGA CCTCAACAGC TCTATTGACA TGACTCGCCT ACCCTCACCT
    ACTAAGGATA AACCATCAAA AGATATGTTT TTTGTTTCAC GTCGCCCGCT AACTCGCTCC
    TCGCCAGCCT ATTGCACCAG CAGCTCAGAC ATCACAGAAC CAGACCAGAA GATGCTGAGC
    GTGAACAAGA GTGTGTCCAT GATGGACTTG CAGGACAGCC GAATGAATAG CGTAACCAAC
    TTGCAGGCCG TAGGTGATCT GCTGAACTCC AGCCAGGCCT CCATATCAGG GGGCCTACGT
    CCGGGGGGCC GGCTCTCCCA GGGCAGTGGA TCCAGCATTG CAGCGGGTCT GAGGCTCAGT
    CAGATGGGGG TCACCACTGA TGGGCTCTCA GCTCAACAGC TACGCATTCC CTTGTCCTTC
    CAGAACCCGC TCTTCCACAT GGCTCCCGAT GGGCCTGGTG GGCCCCCATC TCATCCTGCT
    CCTCATCGAC CAGACCCCGG AGCTGATGCC TTTGCCCCTT TCCATGGGTA TAGCAAGAGT
    GAGGACTTGA GCTCCGGAAA GCATATGCTT CACAGTCACA GCTACAGCGA CGAATTCAGC
    CGGCAGGGGT CAGAGTTCAG TAAAAGGCAG TTATCCCTCC AGGACAATCT CCAGCACATG
    CTTTCACCCC CTCAAATAAC CATTGGCCCT GGAGTGCCAC GAAGGCAAGA CTCACAGCCT
    CCCCCTTTGC AGAGGGGAAA ATCCCAACAA CTGACGGTGC AGGCAGCGCA TAAACCTCGG
    CCAAGCAGTG GCAACCTCCT ACAGAGCCCA GAACCAATGT ATGGGGCAGC AAGAATTAGG
    CAGCAGTCAG TTGAAAAGGA TGCAGGTGGC TTGAAACCAT CAATAACCAA GCAGCACTCC
    CATGCACAGG CCACTCTGAA CCCTGTCACT CCAGCCTCAG AACGCACAGT TGCCTGGGTA
    TCCAACATGC CCCACCTTTC AGCAGATATT GAGAGCGCTC ACATAGAACG GGAGGAGTAT
    AAGTTGAAGG AATACTCTAA ATCAATGGAT GAGAGCCGTC TTGAGAGGGT GAAAGAGTAT
    GAGGAAGAGA TTTACTCCTT AAAAGAGAGG CTGTTGATGT CAAACCGGAA GCTGGAGGAA
    TATGAGAGGC GTCTTCTTAC ACAGGAAGAA CAAACTAATA AGATGATGCA GCAATATCAG
    TCACGCCTGG ACCAGAGTGA AAAGCGTTTG CGACAGCAGC AGGTTGAGAA GGACACGCAG
    ATAAAGAGCA TCTTAAACAG GTTAGAAATG GTTGATGCTG GTGGAGGAGG AGATCAGGAA
    GGATCACTCA CAAGAACCAA TCGATATGAA GAGGAGACTT ATCGAGGCTC AGGTCTGAAC
    TGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031748238.1
    CDS665..4210
    Translation

    Target ORF information:

    RefSeq Version XM_031892378.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031892378.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGATGTTCT ATGCTCCCTT CAGAGATGCT CGAGGACCTC CCATGCATCG TATGCCCTAT 
    GTCACCTCTC CATACGACCG TCCAGCCTGG AACCCAAGAT ACTGTGTCCT CTCTGGAAAT
    CAGCTTCTCA TGCTTGATGA GGAGGAGGTT CACCCGCTGT TGATACGTGA AAGGAGAACT
    GATCCAACCA GAACAAAGTT CCTAAGGCGC ACAGTCAGTG TCCCCGTGGA GGGGAAGCAG
    CCTGACGAAC ACGATTATCA CCTTGCACGT TCCCGGAGGA AAAGCGTCCC AGGGGGAAAA
    CAGCCCAGTA TGGACGCCCC TCCCACCGCC CCCTTTCGAC CGTCGCAAGG ATTCCTGAGC
    CGTCGCTTGA AGAGCTCTAT AAAGAGGACA AAGAGTCAGC CCAAATTAGA TAGGACCAGC
    AGTTTCCGGC AAATATTGCC AAGGTTTCGA AGTGCTGATC ATGATAGGGC ACGGCTCATG
    CAGGCCTTTA AAGAGTCACA CTCTCACGAG TCCTTGCTTA GTCCTAGCAG TGCTGCAGAG
    GCTTTGGATT TGAACTTGGA TGAGGATTCT GTGGTGAAGC CTGTACACAG CAGCATCTTG
    GGACAGGAGT TTTGCTTCGA GGTGACCACA TCATCAGGTA CCAAATGTTT TGCCTGCCGT
    TCTGCTGCTG AAAGAGACAA ATGGATTGAG AATCTTCAAA GAGCTGTAAA GCCCAATAAG
    GATAACAGCA GGCGGGTAGA TAATGTCTTA AAGCTGTGGA TTATTGAAGC CCGTGATCTT
    CCCCCCAAGA AGCGCTATTA TTGTGAGCTC TGTTTGGATG ACATTTTGTA TGCCCGTACC
    ACCAGCAAGC CTCGGAATGA CACTGTCTTT TGGGGGGAAC ACTTTGAATT TAATAACCTT
    CCAGCTGTTC GGAACTTGCG CTTGCACCTC TATAAGGACA CAGACAAGAA AAGGCGCAAA
    GAAAAGAGCA ACTATGTGGG CATGGTGAAC GTGCCTATTG CCAGCATTAG TGGGCGCCAC
    TTTGTGGAGC AGTGGTACCC GGTTTCTCAG CCCACATCCT ACAAGGGGAA GAGCAGCTGT
    CCCTCCATTC GCCTAAAATC CCGATACCAA ACCATGAGCA TCCTCCCCAT GGAGCTGTAC
    AAGGAGTTTG CAGAGTATGT CACCAACAAC TACCGGATGC TGTGCGCTGT ACTGGAGCCT
    GTGCTTAGCG TGAAGAGCAA AGAGGAGGTT GCCTGTGCTT TAGTGCATAT ACTGCAAAGC
    ACAGGCAAGG CCAAGGACTT CCTTTCAGAC ATGGCTATGA CCGAGGTTGA TCGCTTCCTT
    GACAGAGAGC ACCTTATTCT CCGTGAGAAC ACACTTGCAA CTAAAGCCAT AGAGGAGTAT
    CTGAAACTGA TAGGACAGAG ATACCTCAAG GAAGCCATTG GTGAGTTTAT TCGGGCACTG
    TATGAGTCTG AGGAGGACTG TGAGGTTGAT CCCATGCGTT GTCCACCCTC TGCATTGCCC
    GATCACCAGG CCAATCTCAG GATGTGCTGT GAACTCGCCC TTTGCAAAGT CTTTAACTCT
    CATTGTGTGT TTCCTCGGGA GTTGAAGGAA GTCTTTGCAT CTTGGAGGAT GCGCTGTGCA
    GAGCGCGGCC GGGAAGATAT AGCAGATCGA CTCATCAGTG CCTCTCTCTT CCTCCGATTC
    CTGTGTCCGG CCATTATGTC CCCCAGTCTC TTCAGCCTAA TGCAGGAATA CCCTGATGAG
    CAAACATCTA GGACCCTCAC GCTCATCGCA AAAGTCATCC AGAACCTGGC CAACTTCAAC
    TACAACAGGT TCAGCAGTAA AGAAGAATAT ATGTCCTTTA TGAATGAGTT CCTAGAGACG
    GAGTTGGCGA ATATGCAGAA TTTCCTGTAT GAGATCTCAA ACCTGGATTC GCTCACCAAC
    AGCATTAACT TTGAGGGCTA CATTGACCTT GGGCGGGAGC TGAGTACACT TCATGCCCTG
    CTTTGGGAAG TGATCCCACA ACTTAGTAAG GATGCACTTA TAAAGTTGGG GCCTCTTCCA
    CGCCTCCTTA ATGACATCAG CCTAGCTCTC AGGAACCCCC ACATCCAACG ACAACCAAGC
    CACCAAGAAC GACTGCCACC CCCCAAACCT GTCATTCTGA GGGGACCTTC AGCTGAGCTG
    CAAAGCTACT TGGTGCGAGA CCTCAACAGC TCTATTGACA TGACTCGCCT ACCCTCACCT
    ACTAAGGATA AACCATCAAA AGATATGTTT TTTGTTTCAC GTCGCCCGCT AACTCGCTCC
    TCGCCAGCCT ATTGCACCAG CAGCTCAGAC ATCACAGAAC CAGACCAGAA GATGCTGAGC
    GTGAACAAGA GTGTGTCCAT GATGGACTTG CAGGACAGCC GAATGAATAG CGTAACCAAC
    TTGCAGGCCG TAGGTGATCT GCTGAACTCC AGCCAGGCCT CCATATCAGG GGGCCTACGT
    CCGGGGGGCC GGCTCTCCCA GGGCAGTGGA TCCAGCATTG CAGCGGGTCT GAGGCTCAGT
    CAGATGGGGG TCACCACTGA TGGGCTCTCA GCTCAACAGC TACGCATTCC CTTGTCCTTC
    CAGAACCCGC TCTTCCACAT GGCTCCCGAT GGGCCTGGTG GGCCCCCATC TCATCCTGCT
    CCTCATCGAC CAGACCCCGG AGCTGATGCC TTTGCCCCTT TCCATGGGTA TAGCAAGAGT
    GAGGACTTGA GCTCCGGAAA GCATATGCTT CACAGTCACA GCTACAGCGA CGAATTCAGC
    CGGCAGGGGT CAGAGTTCAG TAAAAGGCAG TTATCCCTCC AGGACAATCT CCAGCACATG
    CTTTCACCCC CTCAAATAAC CATTGGCCCT GGAGTGCCAC GAAGGCAAGA CTCACAGCCT
    CCCCCTTTGC AGAGGGGAAA ATCCCAACAA CTGACGGTGC AGGCAGCGCA TAAACCTCGG
    CCAAGCAGTG GCAACCTCCT ACAGAGCCCA GAACCAATGT ATGGGGCAGC AAGAATTAGG
    CAGCAGTCAG TTGAAAAGGA TGCAGGTGGC TTGAAACCAT CAATAACCAA GCAGCACTCC
    CATGCACAGG CCACTCTGAA CCCTGTCACT CCAGCCTCAG AACGCACAGT TGCCTGGGTA
    TCCAACATGC CCCACCTTTC AGCAGATATT GAGAGCGCTC ACATAGAACG GGAGGAGTAT
    AAGTTGAAGG AATACTCTAA ATCAATGGAT GAGAGCCGTC TTGAGAGGGT GAAAGAGTAT
    GAGGAAGAGA TTTACTCCTT AAAAGAGAGG CTGTTGATGT CAAACCGGAA GCTGGAGGAA
    TATGAGAGGC GTCTTCTTAC ACAGGAAGAA CAAACTAATA AGATGATGCA GCAATATCAG
    TCACGCCTGG ACCAGAGTGA AAAGCGTTTG CGACAGCAGC AGGTTGAGAA GGACACGCAG
    ATAAAGAGCA TCTTAAACAG GTTAGAAATG GTTGATGCTG GTGGAGGAGG AGATCAGGAA
    GGATCACTCA CAAGAACCAA TCGATATGAA GAGGAGACTT ATCGAGGCTC AGGTCTGAAC
    TGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa65732
    Clone ID Related Accession (Same CDS sequence) XM_031892379.1
    Accession Version XM_031892379.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3528bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ras/Rap GTPase-activating protein SynGAP isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGATGTTCT ATGCTCCCTT CAGAGATGCT CGAGGACCTC CCATGCATCG TATGCCCTAT 
    GTCACCTCTC CATACGACCG TCCAGCCTGG AACCCAAGAT ACTGTGTCCT CTCTGGAAAT
    CAGCTTCTCA TGCTTGATGA GGAGGAGGTT CACCCGCTGT TGATACGTGA AAGGAGAACT
    GATCCAACCA GAACAAAGTT CCTAAGGCGC ACAGTCAGTG TCCCCGTGGA GGGGAAGCAG
    CCTGACGAAC ACGATTATCA CCTTGCACGT TCCCGGAGGA AAAGCGTCCC AGGGGGAAAA
    CAGCCCAGTA TGGACGCCCC TCCCACCGCC CCCTTTCGAC CGTCGCAAGG ATTCCTGAGC
    CGTCGCTTGA AGAGCTCTAT AAAGAGGACA AAGAGTCAGC CCAAATTAGA TAGGACCAGC
    AGTTTCCGGC AAATATTGCC AAGGTTTCGA AGTGCTGATC ATGATAGGGC ACGGCTCATG
    CAGGCCTTTA AAGAGTCACA CTCTCACGAG TCCTTGCTTA GTCCTAGCAG TGCTGCAGAG
    GCTTTGGATT TGAACTTGGA TGAGGATTCT GTGGTGAAGC CTGTACACAG CAGCATCTTG
    GGACAGGAGT TTTGCTTCGA GGTGACCACA TCATCAGGTA CCAAATGTTT TGCCTGCCGT
    TCTGCTGCTG AAAGAGACAA ATGGATTGAG AATCTTCAAA GAGCTGTAAA GCCCAATAAG
    GATAACAGCA GGCGGGTAGA TAATGTCTTA AAGCTGTGGA TTATTGAAGC CCGTGATCTT
    CCCCCCAAGA AGCGCTATTA TTGTGAGCTC TGTTTGGATG ACATTTTGTA TGCCCGTACC
    ACCAGCAAGC CTCGGAATGA CACTGTCTTT TGGGGGGAAC ACTTTGAATT TAATAACCTT
    CCAGCTGTTC GGAACTTGCG CTTGCACCTC TATAAGGACA CAGACAAGAA AAGGCGCAAA
    GAAAAGAGCA ACTATGTGGG CATGGTGAAC GTGCCTATTG CCAGCATTAG TGGGCGCCAC
    TTTGTGGAGC AGTGGTACCC GGTTTCTCAG CCCACATCCT ACAAGGGGAA GAGCAGCTGT
    CCCTCCATTC GCCTAAAATC CCGATACCAA ACCATGAGCA TCCTCCCCAT GGAGCTGTAC
    AAGGAGTTTG CAGAGTATGT CACCAACAAC TACCGGATGC TGTGCGCTGT ACTGGAGCCT
    GTGCTTAGCG TGAAGAGCAA AGAGGAGGTT GCCTGTGCTT TAGTGCATAT ACTGCAAAGC
    ACAGGCAAGG CCAAGGACTT CCTTTCAGAC ATGGCTATGA CCGAGGTTGA TCGCTTCCTT
    GACAGAGAGC ACCTTATTCT CCGTGAGAAC ACACTTGCAA CTAAAGCCAT AGAGGAGTAT
    CTGAAACTGA TAGGACAGAG ATACCTCAAG GAAGCCATTG GTGAGTTTAT TCGGGCACTG
    TATGAGTCTG AGGAGGACTG TGAGGTTGAT CCCATGCGTT GTCCACCCTC TGCATTGCCC
    GATCACCAGG CCAATCTCAG GATGTGCTGT GAACTCGCCC TTTGCAAAGT CTTTAACTCT
    CATTGTGTGT TTCCTCGGGA GTTGAAGGAA GTCTTTGCAT CTTGGAGGAT GCGCTGTGCA
    GAGCGCGGCC GGGAAGATAT AGCAGATCGA CTCATCAGTG CCTCTCTCTT CCTCCGATTC
    CTGTGTCCGG CCATTATGTC CCCCAGTCTC TTCAGCCTAA TGCAGGAATA CCCTGATGAG
    CAAACATCTA GGACCCTCAC GCTCATCGCA AAAGTCATCC AGAACCTGGC CAACTTCAAC
    TACAACAGGT TCAGCAGTAA AGAAGAATAT ATGTCCTTTA TGAATGAGTT CCTAGAGACG
    GAGTTGGCGA ATATGCAGAA TTTCCTGTAT GAGATCTCAA ACCTGGATTC GCTCACCAAC
    AGCATTAACT TTGAGGGCTA CATTGACCTT GGGCGGGAGC TGAGTACACT TCATGCCCTG
    CTTTGGGAAG TGATCCCACA ACTTAGTAAG GATGCACTTA TAAAGTTGGG GCCTCTTCCA
    CGCCTCCTTA ATGACATCAG CCTAGCTCTC AGGAACCCCC ACATCCAACG ACAACCAAGC
    CACCAAGAAC GACTGCCACC CCCCAAACCT GTCATTCTGA GGGGACCTTC AGCTGAGCTG
    CAAAGCTACT TGGTGCGAGA CCTCAACAGC TCTATTGACA TGACTCGCCT ACCCTCACCT
    ACTAAGGATA AACCATCAAA AGATATGTTT TTTGTTTCAC GTCGCCCGCT AACTCGCTCC
    TCGCCAGCCT ATTGCACCAG CAGCTCAGAC ATCACAGAAC CAGACCAGAA GATGCTGAGC
    GTGAACAAGA GTGTGTCCAT GATGGACTTG CAGGACAGCC GAATGAATAG CGTAACCAAC
    TTGCAGGCCG TAGGTGATCT GCTGAACTCC AGCCAGGCCT CCATATCAGG GGGCCTACGT
    CCGGGGGGCC GGCTCTCCCA GGGCAGTGGA TCCAGCATTG CAGCGGGTCT GAGGCTCAGT
    CAGATGGGGG TCACCACTGA TGGGCTCTCA GCTCAACAGC TACGCATTCC CTTGTCCTTC
    CAGAACCCGC TCTTCCACAT GGCTCCCGAT GGGCCTGGTG GGCCCCCATC TCATCCTGCT
    CCTCATCGAC CAGACCCCGG AGCTGATGCC TTTGCCCCTT TCCATGGGTA TAGCAAGAGT
    GAGGACTTGA GCTCCGGAAA GCATATGCTT CACAGTCACA GCTACAGCGA CGAATTCAGC
    CGGCAGGGGT CAGAGTTCAG TAAAAGGCAG TTATCCCTCC AGGACAATCT CCAGCACATG
    CTTTCACCCC CTCAAATAAC CATTGGCCCT GGAGTGCCAC GAAGGCAAGA CTCACAGCCT
    CCCCCTTTGC AGAGGGGAAA ATCCCAACAA CTGACGGTGC AGGCAGCGCA TAAACCTCGG
    CCAAGCAGTG GCAACCTCCT ACAGAGCCCA GAACCAATGT ATGGGGCAGC AAGAATTAGG
    CAGCAGTCAG TTGAAAAGGA TGCAGGTGGC TTGAAACCAT CAATAACCAA GCAGCACTCC
    CATGCACAGG CCACTCTGAA CCCTGTCACT CCAGCCTCAG AACGCACAGT TGCCTGGGTA
    TCCAACATGC CCCACCTTTC AGCAGATATT GAGAGCGCTC ACATAGAACG GGAGGAGTAT
    AAGTTGAAGG AATACTCTAA ATCAATGGAT GAGAGCCGTC TTGAGAGGGT GAAAGAGTAT
    GAGGAAGAGA TTTACTCCTT AAAAGAGAGG CTGTTGATGT CAAACCGGAA GCTGGAGGAA
    TATGAGAGGC GTCTTCTTAC ACAGGAAGAA CAAACTAATA AGATGATGCA GCAATATCAG
    TCACGCCTGG ACCAGAGTGA AAAGCGTTTG CGACAGCAGC AGGTTGAGAA GGACACGCAG
    ATAAAGAGCA TCTTAAACAG GTTGATGCTG GTGGAGGAGG AGATCAGGAA GGATCACTCA
    CAAGAACCAA TCGATATGAA GAGGAGACTT ATCGAGGCTC AGGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031748239.1
    CDS665..4192
    Translation

    Target ORF information:

    RefSeq Version XM_031892379.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031892379.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGATGTTCT ATGCTCCCTT CAGAGATGCT CGAGGACCTC CCATGCATCG TATGCCCTAT 
    GTCACCTCTC CATACGACCG TCCAGCCTGG AACCCAAGAT ACTGTGTCCT CTCTGGAAAT
    CAGCTTCTCA TGCTTGATGA GGAGGAGGTT CACCCGCTGT TGATACGTGA AAGGAGAACT
    GATCCAACCA GAACAAAGTT CCTAAGGCGC ACAGTCAGTG TCCCCGTGGA GGGGAAGCAG
    CCTGACGAAC ACGATTATCA CCTTGCACGT TCCCGGAGGA AAAGCGTCCC AGGGGGAAAA
    CAGCCCAGTA TGGACGCCCC TCCCACCGCC CCCTTTCGAC CGTCGCAAGG ATTCCTGAGC
    CGTCGCTTGA AGAGCTCTAT AAAGAGGACA AAGAGTCAGC CCAAATTAGA TAGGACCAGC
    AGTTTCCGGC AAATATTGCC AAGGTTTCGA AGTGCTGATC ATGATAGGGC ACGGCTCATG
    CAGGCCTTTA AAGAGTCACA CTCTCACGAG TCCTTGCTTA GTCCTAGCAG TGCTGCAGAG
    GCTTTGGATT TGAACTTGGA TGAGGATTCT GTGGTGAAGC CTGTACACAG CAGCATCTTG
    GGACAGGAGT TTTGCTTCGA GGTGACCACA TCATCAGGTA CCAAATGTTT TGCCTGCCGT
    TCTGCTGCTG AAAGAGACAA ATGGATTGAG AATCTTCAAA GAGCTGTAAA GCCCAATAAG
    GATAACAGCA GGCGGGTAGA TAATGTCTTA AAGCTGTGGA TTATTGAAGC CCGTGATCTT
    CCCCCCAAGA AGCGCTATTA TTGTGAGCTC TGTTTGGATG ACATTTTGTA TGCCCGTACC
    ACCAGCAAGC CTCGGAATGA CACTGTCTTT TGGGGGGAAC ACTTTGAATT TAATAACCTT
    CCAGCTGTTC GGAACTTGCG CTTGCACCTC TATAAGGACA CAGACAAGAA AAGGCGCAAA
    GAAAAGAGCA ACTATGTGGG CATGGTGAAC GTGCCTATTG CCAGCATTAG TGGGCGCCAC
    TTTGTGGAGC AGTGGTACCC GGTTTCTCAG CCCACATCCT ACAAGGGGAA GAGCAGCTGT
    CCCTCCATTC GCCTAAAATC CCGATACCAA ACCATGAGCA TCCTCCCCAT GGAGCTGTAC
    AAGGAGTTTG CAGAGTATGT CACCAACAAC TACCGGATGC TGTGCGCTGT ACTGGAGCCT
    GTGCTTAGCG TGAAGAGCAA AGAGGAGGTT GCCTGTGCTT TAGTGCATAT ACTGCAAAGC
    ACAGGCAAGG CCAAGGACTT CCTTTCAGAC ATGGCTATGA CCGAGGTTGA TCGCTTCCTT
    GACAGAGAGC ACCTTATTCT CCGTGAGAAC ACACTTGCAA CTAAAGCCAT AGAGGAGTAT
    CTGAAACTGA TAGGACAGAG ATACCTCAAG GAAGCCATTG GTGAGTTTAT TCGGGCACTG
    TATGAGTCTG AGGAGGACTG TGAGGTTGAT CCCATGCGTT GTCCACCCTC TGCATTGCCC
    GATCACCAGG CCAATCTCAG GATGTGCTGT GAACTCGCCC TTTGCAAAGT CTTTAACTCT
    CATTGTGTGT TTCCTCGGGA GTTGAAGGAA GTCTTTGCAT CTTGGAGGAT GCGCTGTGCA
    GAGCGCGGCC GGGAAGATAT AGCAGATCGA CTCATCAGTG CCTCTCTCTT CCTCCGATTC
    CTGTGTCCGG CCATTATGTC CCCCAGTCTC TTCAGCCTAA TGCAGGAATA CCCTGATGAG
    CAAACATCTA GGACCCTCAC GCTCATCGCA AAAGTCATCC AGAACCTGGC CAACTTCAAC
    TACAACAGGT TCAGCAGTAA AGAAGAATAT ATGTCCTTTA TGAATGAGTT CCTAGAGACG
    GAGTTGGCGA ATATGCAGAA TTTCCTGTAT GAGATCTCAA ACCTGGATTC GCTCACCAAC
    AGCATTAACT TTGAGGGCTA CATTGACCTT GGGCGGGAGC TGAGTACACT TCATGCCCTG
    CTTTGGGAAG TGATCCCACA ACTTAGTAAG GATGCACTTA TAAAGTTGGG GCCTCTTCCA
    CGCCTCCTTA ATGACATCAG CCTAGCTCTC AGGAACCCCC ACATCCAACG ACAACCAAGC
    CACCAAGAAC GACTGCCACC CCCCAAACCT GTCATTCTGA GGGGACCTTC AGCTGAGCTG
    CAAAGCTACT TGGTGCGAGA CCTCAACAGC TCTATTGACA TGACTCGCCT ACCCTCACCT
    ACTAAGGATA AACCATCAAA AGATATGTTT TTTGTTTCAC GTCGCCCGCT AACTCGCTCC
    TCGCCAGCCT ATTGCACCAG CAGCTCAGAC ATCACAGAAC CAGACCAGAA GATGCTGAGC
    GTGAACAAGA GTGTGTCCAT GATGGACTTG CAGGACAGCC GAATGAATAG CGTAACCAAC
    TTGCAGGCCG TAGGTGATCT GCTGAACTCC AGCCAGGCCT CCATATCAGG GGGCCTACGT
    CCGGGGGGCC GGCTCTCCCA GGGCAGTGGA TCCAGCATTG CAGCGGGTCT GAGGCTCAGT
    CAGATGGGGG TCACCACTGA TGGGCTCTCA GCTCAACAGC TACGCATTCC CTTGTCCTTC
    CAGAACCCGC TCTTCCACAT GGCTCCCGAT GGGCCTGGTG GGCCCCCATC TCATCCTGCT
    CCTCATCGAC CAGACCCCGG AGCTGATGCC TTTGCCCCTT TCCATGGGTA TAGCAAGAGT
    GAGGACTTGA GCTCCGGAAA GCATATGCTT CACAGTCACA GCTACAGCGA CGAATTCAGC
    CGGCAGGGGT CAGAGTTCAG TAAAAGGCAG TTATCCCTCC AGGACAATCT CCAGCACATG
    CTTTCACCCC CTCAAATAAC CATTGGCCCT GGAGTGCCAC GAAGGCAAGA CTCACAGCCT
    CCCCCTTTGC AGAGGGGAAA ATCCCAACAA CTGACGGTGC AGGCAGCGCA TAAACCTCGG
    CCAAGCAGTG GCAACCTCCT ACAGAGCCCA GAACCAATGT ATGGGGCAGC AAGAATTAGG
    CAGCAGTCAG TTGAAAAGGA TGCAGGTGGC TTGAAACCAT CAATAACCAA GCAGCACTCC
    CATGCACAGG CCACTCTGAA CCCTGTCACT CCAGCCTCAG AACGCACAGT TGCCTGGGTA
    TCCAACATGC CCCACCTTTC AGCAGATATT GAGAGCGCTC ACATAGAACG GGAGGAGTAT
    AAGTTGAAGG AATACTCTAA ATCAATGGAT GAGAGCCGTC TTGAGAGGGT GAAAGAGTAT
    GAGGAAGAGA TTTACTCCTT AAAAGAGAGG CTGTTGATGT CAAACCGGAA GCTGGAGGAA
    TATGAGAGGC GTCTTCTTAC ACAGGAAGAA CAAACTAATA AGATGATGCA GCAATATCAG
    TCACGCCTGG ACCAGAGTGA AAAGCGTTTG CGACAGCAGC AGGTTGAGAA GGACACGCAG
    ATAAAGAGCA TCTTAAACAG GTTGATGCTG GTGGAGGAGG AGATCAGGAA GGATCACTCA
    CAAGAACCAA TCGATATGAA GAGGAGACTT ATCGAGGCTC AGGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa65733
    Clone ID Related Accession (Same CDS sequence) XM_031892380.1
    Accession Version XM_031892380.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3498bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ras/Rap GTPase-activating protein SynGAP isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGATGTTCT ATGCTCCCTT CAGAGATGCT CGAGGACCTC CCATGCATCG TATGCCCTAT 
    GTCACCTCTC CATACGACCG TCCAGCCTGG AACCCAAGAT ACTGTGTCCT CTCTGGAAAT
    CAGCTTCTCA TGCTTGATGA GGAGGAGGTT CACCCGCTGT TGATACGTGA AAGGAGAACT
    GATCCAACCA GAACAAAGTT CCTAAGGCGC ACAGTCAGTG TCCCCGTGGA GGGGAAGCAG
    CCTGACGAAC ACGATTATCA CCTTGCACGT TCCCGGAGGA AAAGCGTCCC AGGGGGAAAA
    CAGCCCAGTA TGGACGCCCC TCCCACCGCC CCCTTTCGAC CGTCGCAAGG ATTCCTGAGC
    CGTCGCTTGA AGAGCTCTAT AAAGAGGACA AAGAGTCAGC CCAAATTAGA TAGGACCAGC
    AGTTTCCGGC AAATATTGCC AAGGTTTCGA AGTGCTGATC ATGATAGGGC ACGGCTCATG
    CAGGCCTTTA AAGAGTCACA CTCTCACGAG TCCTTGCTTA GTCCTAGCAG TGCTGCAGAG
    GCTTTGGATT TGAACTTGGA TGAGGATTCT GTGGTGAAGC CTGTACACAG CAGCATCTTG
    GGACAGGAGT TTTGCTTCGA GGTGACCACA TCATCAGGTA CCAAATGTTT TGCCTGCCGT
    TCTGCTGCTG AAAGAGACAA ATGGATTGAG AATCTTCAAA GAGCTGTAAA GCCCAATAAG
    GATAACAGCA GGCGGGTAGA TAATGTCTTA AAGCTGTGGA TTATTGAAGC CCGTGATCTT
    CCCCCCAAGA AGCGCTATTA TTGTGAGCTC TGTTTGGATG ACATTTTGTA TGCCCGTACC
    ACCAGCAAGC CTCGGAATGA CACTGTCTTT TGGGGGGAAC ACTTTGAATT TAATAACCTT
    CCAGCTGTTC GGAACTTGCG CTTGCACCTC TATAAGGACA CAGACAAGAA AAGGCGCAAA
    GAAAAGAGCA ACTATGTGGG CATGGTGAAC GTGCCTATTG CCAGCATTAG TGGGCGCCAC
    TTTGTGGAGC AGTGGTACCC GGTTTCTCAG CCCACATCCT ACAAGGGGAA GAGCAGCTGT
    CCCTCCATTC GCCTAAAATC CCGATACCAA ACCATGAGCA TCCTCCCCAT GGAGCTGTAC
    AAGGAGTTTG CAGAGTATGT CACCAACAAC TACCGGATGC TGTGCGCTGT ACTGGAGCCT
    GTGCTTAGCG TGAAGAGCAA AGAGGAGGTT GCCTGTGCTT TAGTGCATAT ACTGCAAAGC
    ACAGGCAAGG CCAAGGACTT CCTTTCAGAC ATGGCTATGA CCGAGGTTGA TCGCTTCCTT
    GACAGAGAGC ACCTTATTCT CCGTGAGAAC ACACTTGCAA CTAAAGCCAT AGAGGAGTAT
    CTGAAACTGA TAGGACAGAG ATACCTCAAG GAAGCCATTG GTGAGTTTAT TCGGGCACTG
    TATGAGTCTG AGGAGGACTG TGAGGTTGAT CCCATGCGTT GTCCACCCTC TGCATTGCCC
    GATCACCAGG CCAATCTCAG GATGTGCTGT GAACTCGCCC TTTGCAAAGT CTTTAACTCT
    CATTGTGTGT TTCCTCGGGA GTTGAAGGAA GTCTTTGCAT CTTGGAGGAT GCGCTGTGCA
    GAGCGCGGCC GGGAAGATAT AGCAGATCGA CTCATCAGTG CCTCTCTCTT CCTCCGATTC
    CTGTGTCCGG CCATTATGTC CCCCAGTCTC TTCAGCCTAA TGCAGGAATA CCCTGATGAG
    CAAACATCTA GGACCCTCAC GCTCATCGCA AAAGTCATCC AGAACCTGGC CAACTTCAAC
    TACAACAGGT TCAGCAGTAA AGAAGAATAT ATGTCCTTTA TGAATGAGTT CCTAGAGACG
    GAGTTGGCGA ATATGCAGAA TTTCCTGTAT GAGATCTCAA ACCTGGATTC GCTCACCAAC
    AGCATTAACT TTGAGGGCTA CATTGACCTT GGGCGGGAGC TGAGTACACT TCATGCCCTG
    CTTTGGGAAG TGATCCCACA ACTTAGTAAG GATGCACTTA TAAAGTTGGG GCCTCTTCCA
    CGCCTCCTTA ATGACATCAG CCTAGCTCTC AGGAACCCCC ACATCCAACG ACAACCAAGC
    CACCAAGAAC GACTGCCACC CCCCAAACCT GTCATTCTGA GGGGACCTTC AGCTGAGCTG
    CAAAGCTACT TGGTGCGAGA CCTCAACAGC TCTATTGACA TGACTCGCCT ACCCTCACCT
    ACTAAGGATA AACCATCAAA AGATATGTTT TTTGTTTCAC GTCGCCCGCT AACTCGCTCC
    TCGCCAGCCT ATTGCACCAG CAGCTCAGAC ATCACAGAAC CAGACCAGAA GATGCTGAGC
    GTGAACAAGA GTGTGTCCAT GATGGACTTG CAGGACAGCC GAATGAATAG CGTAACCAAC
    TTGCAGGCCA ACCCGCTCTT CCACATGGCT CCCGATGGGC CTGGTGGGCC CCCATCTCAT
    CCTGCTCCTC ATCGACCAGA CCCCGGAGCT GATGCCTTTG CCCCTTTCCA TGGGTATAGC
    AAGAGTGAGG ACTTGAGCTC CGGAAAGCAT ATGCTTCACA GTCACAGCTA CAGCGACGAA
    TTCAGCCGGC AGGGGTCAGA GTTCAGTAAA AGGCAGTTAT CCCTCCAGGA CAATCTCCAG
    CACATGCTTT CACCCCCTCA AATAACCATT GGCCCTGGAG TGCCACGAAG GCAAGACTCA
    CAGCCTCCCC CTTTGCAGAG GGGAAAATCC CAACAACTGA CGGTGCAGGC AGCGCATAAA
    CCTCGGCCAA GCAGTGGCAA CCTCCTACAG AGCCCAGAAC CAATGTATGG GGCAGCAAGA
    ATTAGGCAGC AGTCAGTTGA AAAGGATGCA GGTGGCTTGA AACCATCAAT AACCAAGCAG
    CACTCCCATG CACAGGCCAC TCTGAACCCT GTCACTCCAG CCTCAGAACG CACAGTTGCC
    TGGGTATCCA ACATGCCCCA CCTTTCAGCA GATATTGAGA GCGCTCACAT AGAACGGGAG
    GAGTATAAGT TGAAGGAATA CTCTAAATCA ATGGATGAGA GCCGTCTTGA GAGGGTGAAA
    GAGTATGAGG AAGAGATTTA CTCCTTAAAA GAGAGGCTGT TGATGTCAAA CCGGAAGCTG
    GAGGAATATG AGAGGCGTCT TCTTACACAG GAAGAACAAA CTAATAAGAT GATGCAGCAA
    TATCAGTCAC GCCTGGACCA GAGTGAAAAG CGTTTGCGAC AGCAGCAGGT TGAGAAGGAC
    ACGCAGATAA AGAGCATCTT AAACAGGTTA GAAATGGTTG ATGCTGGTGG AGGAGGAGAT
    CAGGAAGGAT CACTCACAAG AACCAATCGA TATGAAGAGG AGACTTATCG AGGCTCAGAG
    AAGAAGTTTC CCTCCGTGGG TACAGCAGAC CCGCGTCTGA GGCTGGCGAC TCCATGGAAT
    GGGGCCCTCC CGCCTCTGCC TCCTCGACTC CAAATCACAG AAAATGGAGA CTTTCTAAAC
    TCAAGTGACC ATCACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031748240.1
    CDS665..4162
    Translation

    Target ORF information:

    RefSeq Version XM_031892380.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031892380.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGATGTTCT ATGCTCCCTT CAGAGATGCT CGAGGACCTC CCATGCATCG TATGCCCTAT 
    GTCACCTCTC CATACGACCG TCCAGCCTGG AACCCAAGAT ACTGTGTCCT CTCTGGAAAT
    CAGCTTCTCA TGCTTGATGA GGAGGAGGTT CACCCGCTGT TGATACGTGA AAGGAGAACT
    GATCCAACCA GAACAAAGTT CCTAAGGCGC ACAGTCAGTG TCCCCGTGGA GGGGAAGCAG
    CCTGACGAAC ACGATTATCA CCTTGCACGT TCCCGGAGGA AAAGCGTCCC AGGGGGAAAA
    CAGCCCAGTA TGGACGCCCC TCCCACCGCC CCCTTTCGAC CGTCGCAAGG ATTCCTGAGC
    CGTCGCTTGA AGAGCTCTAT AAAGAGGACA AAGAGTCAGC CCAAATTAGA TAGGACCAGC
    AGTTTCCGGC AAATATTGCC AAGGTTTCGA AGTGCTGATC ATGATAGGGC ACGGCTCATG
    CAGGCCTTTA AAGAGTCACA CTCTCACGAG TCCTTGCTTA GTCCTAGCAG TGCTGCAGAG
    GCTTTGGATT TGAACTTGGA TGAGGATTCT GTGGTGAAGC CTGTACACAG CAGCATCTTG
    GGACAGGAGT TTTGCTTCGA GGTGACCACA TCATCAGGTA CCAAATGTTT TGCCTGCCGT
    TCTGCTGCTG AAAGAGACAA ATGGATTGAG AATCTTCAAA GAGCTGTAAA GCCCAATAAG
    GATAACAGCA GGCGGGTAGA TAATGTCTTA AAGCTGTGGA TTATTGAAGC CCGTGATCTT
    CCCCCCAAGA AGCGCTATTA TTGTGAGCTC TGTTTGGATG ACATTTTGTA TGCCCGTACC
    ACCAGCAAGC CTCGGAATGA CACTGTCTTT TGGGGGGAAC ACTTTGAATT TAATAACCTT
    CCAGCTGTTC GGAACTTGCG CTTGCACCTC TATAAGGACA CAGACAAGAA AAGGCGCAAA
    GAAAAGAGCA ACTATGTGGG CATGGTGAAC GTGCCTATTG CCAGCATTAG TGGGCGCCAC
    TTTGTGGAGC AGTGGTACCC GGTTTCTCAG CCCACATCCT ACAAGGGGAA GAGCAGCTGT
    CCCTCCATTC GCCTAAAATC CCGATACCAA ACCATGAGCA TCCTCCCCAT GGAGCTGTAC
    AAGGAGTTTG CAGAGTATGT CACCAACAAC TACCGGATGC TGTGCGCTGT ACTGGAGCCT
    GTGCTTAGCG TGAAGAGCAA AGAGGAGGTT GCCTGTGCTT TAGTGCATAT ACTGCAAAGC
    ACAGGCAAGG CCAAGGACTT CCTTTCAGAC ATGGCTATGA CCGAGGTTGA TCGCTTCCTT
    GACAGAGAGC ACCTTATTCT CCGTGAGAAC ACACTTGCAA CTAAAGCCAT AGAGGAGTAT
    CTGAAACTGA TAGGACAGAG ATACCTCAAG GAAGCCATTG GTGAGTTTAT TCGGGCACTG
    TATGAGTCTG AGGAGGACTG TGAGGTTGAT CCCATGCGTT GTCCACCCTC TGCATTGCCC
    GATCACCAGG CCAATCTCAG GATGTGCTGT GAACTCGCCC TTTGCAAAGT CTTTAACTCT
    CATTGTGTGT TTCCTCGGGA GTTGAAGGAA GTCTTTGCAT CTTGGAGGAT GCGCTGTGCA
    GAGCGCGGCC GGGAAGATAT AGCAGATCGA CTCATCAGTG CCTCTCTCTT CCTCCGATTC
    CTGTGTCCGG CCATTATGTC CCCCAGTCTC TTCAGCCTAA TGCAGGAATA CCCTGATGAG
    CAAACATCTA GGACCCTCAC GCTCATCGCA AAAGTCATCC AGAACCTGGC CAACTTCAAC
    TACAACAGGT TCAGCAGTAA AGAAGAATAT ATGTCCTTTA TGAATGAGTT CCTAGAGACG
    GAGTTGGCGA ATATGCAGAA TTTCCTGTAT GAGATCTCAA ACCTGGATTC GCTCACCAAC
    AGCATTAACT TTGAGGGCTA CATTGACCTT GGGCGGGAGC TGAGTACACT TCATGCCCTG
    CTTTGGGAAG TGATCCCACA ACTTAGTAAG GATGCACTTA TAAAGTTGGG GCCTCTTCCA
    CGCCTCCTTA ATGACATCAG CCTAGCTCTC AGGAACCCCC ACATCCAACG ACAACCAAGC
    CACCAAGAAC GACTGCCACC CCCCAAACCT GTCATTCTGA GGGGACCTTC AGCTGAGCTG
    CAAAGCTACT TGGTGCGAGA CCTCAACAGC TCTATTGACA TGACTCGCCT ACCCTCACCT
    ACTAAGGATA AACCATCAAA AGATATGTTT TTTGTTTCAC GTCGCCCGCT AACTCGCTCC
    TCGCCAGCCT ATTGCACCAG CAGCTCAGAC ATCACAGAAC CAGACCAGAA GATGCTGAGC
    GTGAACAAGA GTGTGTCCAT GATGGACTTG CAGGACAGCC GAATGAATAG CGTAACCAAC
    TTGCAGGCCA ACCCGCTCTT CCACATGGCT CCCGATGGGC CTGGTGGGCC CCCATCTCAT
    CCTGCTCCTC ATCGACCAGA CCCCGGAGCT GATGCCTTTG CCCCTTTCCA TGGGTATAGC
    AAGAGTGAGG ACTTGAGCTC CGGAAAGCAT ATGCTTCACA GTCACAGCTA CAGCGACGAA
    TTCAGCCGGC AGGGGTCAGA GTTCAGTAAA AGGCAGTTAT CCCTCCAGGA CAATCTCCAG
    CACATGCTTT CACCCCCTCA AATAACCATT GGCCCTGGAG TGCCACGAAG GCAAGACTCA
    CAGCCTCCCC CTTTGCAGAG GGGAAAATCC CAACAACTGA CGGTGCAGGC AGCGCATAAA
    CCTCGGCCAA GCAGTGGCAA CCTCCTACAG AGCCCAGAAC CAATGTATGG GGCAGCAAGA
    ATTAGGCAGC AGTCAGTTGA AAAGGATGCA GGTGGCTTGA AACCATCAAT AACCAAGCAG
    CACTCCCATG CACAGGCCAC TCTGAACCCT GTCACTCCAG CCTCAGAACG CACAGTTGCC
    TGGGTATCCA ACATGCCCCA CCTTTCAGCA GATATTGAGA GCGCTCACAT AGAACGGGAG
    GAGTATAAGT TGAAGGAATA CTCTAAATCA ATGGATGAGA GCCGTCTTGA GAGGGTGAAA
    GAGTATGAGG AAGAGATTTA CTCCTTAAAA GAGAGGCTGT TGATGTCAAA CCGGAAGCTG
    GAGGAATATG AGAGGCGTCT TCTTACACAG GAAGAACAAA CTAATAAGAT GATGCAGCAA
    TATCAGTCAC GCCTGGACCA GAGTGAAAAG CGTTTGCGAC AGCAGCAGGT TGAGAAGGAC
    ACGCAGATAA AGAGCATCTT AAACAGGTTA GAAATGGTTG ATGCTGGTGG AGGAGGAGAT
    CAGGAAGGAT CACTCACAAG AACCAATCGA TATGAAGAGG AGACTTATCG AGGCTCAGAG
    AAGAAGTTTC CCTCCGTGGG TACAGCAGAC CCGCGTCTGA GGCTGGCGAC TCCATGGAAT
    GGGGCCCTCC CGCCTCTGCC TCCTCGACTC CAAATCACAG AAAATGGAGA CTTTCTAAAC
    TCAAGTGACC ATCACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa65734
    Clone ID Related Accession (Same CDS sequence) XM_031892381.1
    Accession Version XM_031892381.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ras/Rap GTPase-activating protein SynGAP isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGATGTTCT ATGCTCCCTT CAGAGATGCT CGAGGACCTC CCATGCATCG TATGCCCTAT 
    GTCACCTCTC CATACGACCG TCCAGCCTGG AACCCAAGAT ACTGTGTCCT CTCTGGAAAT
    CAGCTTCTCA TGCTTGATGA GGAGGAGGTT CACCCGCTGT TGATACGTGA AAGGAGAACT
    GATCCAACCA GAACAAAGTT CCTAAGGCGC ACAGTCAGTG TCCCCGTGGA GGGGAAGCAG
    CCTGACGAAC ACGATTATCA CCTTGCACGT TCCCGGAGGA AAAGCGTCCC AGGGGGAAAA
    CAGCCCAGTA TGGACGCCCC TCCCACCGCC CCCTTTCGAC CGTCGCAAGG ATTCCTGAGC
    CGTCGCTTGA AGAGCTCTAT AAAGAGGACA AAGAGTCAGC CCAAATTAGA TAGGACCAGC
    AGTTTCCGGC AAATATTGCC AAGGTTTCGA AGTGCTGATC ATGATAGGGC ACGGCTCATG
    CAGGCCTTTA AAGAGTCACA CTCTCACGAG TCCTTGCTTA GTCCTAGCAG TGCTGCAGAG
    GCTTTGGATT TGAACTTGGA TGAGGATTCT GTGGTGAAGC CTGTACACAG CAGCATCTTG
    GGACAGGAGT TTTGCTTCGA GGTGACCACA TCATCAGGTA CCAAATGTTT TGCCTGCCGT
    TCTGCTGCTG AAAGAGACAA ATGGATTGAG AATCTTCAAA GAGCTGTAAA GCCCAATAAG
    GATAACAGCA GGCGGGTAGA TAATGTCTTA AAGCTGTGGA TTATTGAAGC CCGTGATCTT
    CCCCCCAAGA AGCGCTATTA TTGTGAGCTC TGTTTGGATG ACATTTTGTA TGCCCGTACC
    ACCAGCAAGC CTCGGAATGA CACTGTCTTT TGGGGGGAAC ACTTTGAATT TAATAACCTT
    CCAGCTGTTC GGAACTTGCG CTTGCACCTC TATAAGGACA CAGACAAGAA AAGGCGCAAA
    GAAAAGAGCA ACTATGTGGG CATGGTGAAC GTGCCTATTG CCAGCATTAG TGGGCGCCAC
    TTTGTGGAGC AGTGGTACCC GGTTTCTCAG CCCACATCCT ACAAGGGGAA GAGCAGCTGT
    CCCTCCATTC GCCTAAAATC CCGATACCAA ACCATGAGCA TCCTCCCCAT GGAGCTGTAC
    AAGGAGTTTG CAGAGTATGT CACCAACAAC TACCGGATGC TGTGCGCTGT ACTGGAGCCT
    GTGCTTAGCG TGAAGAGCAA AGAGGAGGTT GCCTGTGCTT TAGTGCATAT ACTGCAAAGC
    ACAGGCAAGG CCAAGGACTT CCTTTCAGAC ATGGCTATGA CCGAGGTTGA TCGCTTCCTT
    GACAGAGAGC ACCTTATTCT CCGTGAGAAC ACACTTGCAA CTAAAGCCAT AGAGGAGTAT
    CTGAAACTGA TAGGACAGAG ATACCTCAAG GAAGCCATTG GTGAGTTTAT TCGGGCACTG
    TATGAGTCTG AGGAGGACTG TGAGGTTGAT CCCATGCGTT GTCCACCCTC TGCATTGCCC
    GATCACCAGG CCAATCTCAG GATGTGCTGT GAACTCGCCC TTTGCAAAGT CTTTAACTCT
    CATTGTGTGT TTCCTCGGGA GTTGAAGGAA GTCTTTGCAT CTTGGAGGAT GCGCTGTGCA
    GAGCGCGGCC GGGAAGATAT AGCAGATCGA CTCATCAGTG CCTCTCTCTT CCTCCGATTC
    CTGTGTCCGG CCATTATGTC CCCCAGTCTC TTCAGCCTAA TGCAGGAATA CCCTGATGAG
    CAAACATCTA GGACCCTCAC GCTCATCGCA AAAGTCATCC AGAACCTGGC CAACTTCAAC
    TACAAGTTCA GCAGTAAAGA AGAATATATG TCCTTTATGA ATGAGTTCCT AGAGACGGAG
    TTGGCGAATA TGCAGAATTT CCTGTATGAG ATCTCAAACC TGGATTCGCT CACCAACAGC
    ATTAACTTTG AGGGCTACAT TGACCTTGGG CGGGAGCTGA GTACACTTCA TGCCCTGCTT
    TGGGAAGTGA TCCCACAACT TAGTAAGGAT GCACTTATAA AGTTGGGGCC TCTTCCACGC
    CTCCTTAATG ACATCAGCCT AGCTCTCAGG AACCCCCACA TCCAACGACA ACCAAGCCAC
    CAAGAACGAC TGCCACCCCC CAAACCTGTC ATTCTGAGGG GACCTTCAGC TGAGCTGCAA
    AGCTACTTGG TGCGAGACCT CAACAGCTCT ATTGACATGA CTCGCCTACC CTCACCTACT
    AAGGATAAAC CATCAAAAGA TATGTTTTTT GTTTCACGTC GCCCGCTAAC TCGCTCCTCG
    CCAGCCTATT GCACCAGCAG CTCAGACATC ACAGAACCAG ACCAGAAGAT GCTGAGCGTG
    AACAAGAGTG TGTCCATGAT GGACTTGCAG GACAGCCGAA TGAATAGCGT AACCAACTTG
    CAGGCCAACC CGCTCTTCCA CATGGCTCCC GATGGGCCTG GTGGGCCCCC ATCTCATCCT
    GCTCCTCATC GACCAGACCC CGGAGCTGAT GCCTTTGCCC CTTTCCATGG GTATAGCAAG
    AGTGAGGACT TGAGCTCCGG AAAGCATATG CTTCACAGTC ACAGCTACAG CGACGAATTC
    AGCCGGCAGG GGTCAGAGTT CAGTAAAAGG CAGTTATCCC TCCAGGACAA TCTCCAGCAC
    ATGCTTTCAC CCCCTCAAAT AACCATTGGC CCTGGAGTGC CACGAAGGCA AGACTCACAG
    CCTCCCCCTT TGCAGAGGGG AAAATCCCAA CAACTGACGG TGCAGGCAGC GCATAAACCT
    CGGCCAAGCA GTGGCAACCT CCTACAGAGC CCAGAACCAA TGTATGGGGC AGCAAGAATT
    AGGCAGCAGT CAGTTGAAAA GGATGCAGGT GGCTTGAAAC CATCAATAAC CAAGCAGCAC
    TCCCATGCAC AGGCCACTCT GAACCCTGTC ACTCCAGCCT CAGAACGCAC AGTTGCCTGG
    GTATCCAACA TGCCCCACCT TTCAGCAGAT ATTGAGAGCG CTCACATAGA ACGGGAGGAG
    TATAAGTTGA AGGAATACTC TAAATCAATG GATGAGAGCC GTCTTGAGAG GGTGAAAGAG
    TATGAGGAAG AGATTTACTC CTTAAAAGAG AGGCTGTTGA TGTCAAACCG GAAGCTGGAG
    GAATATGAGA GGCGTCTTCT TACACAGGAA GAACAAACTA ATAAGATGAT GCAGCAATAT
    CAGTCACGCC TGGACCAGAG TGAAAAGCGT TTGCGACAGC AGCAGGTTGA GAAGGACACG
    CAGATAAAGA GCATCTTAAA CAGGTTAGAA ATGGTTGATG CTGGTGGAGG AGGAGATCAG
    GAAGGATCAC TCACAAGAAC CAATCGATAT GAAGAGGAGA CTTATCGAGG CTCAGAGAAG
    AAGTTTCCCT CCGTGGGTAC AGCAGACCCG CGTCTGAGGC TGGCGACTCC ATGGAATGGG
    GCCCTCCCGC CTCTGCCTCC TCGACTCCAA ATCACAGAAA ATGGAGACTT TCTAAACTCA
    AGTGACCATC ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031748241.1
    CDS665..4159
    Translation

    Target ORF information:

    RefSeq Version XM_031892381.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031892381.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGATGTTCT ATGCTCCCTT CAGAGATGCT CGAGGACCTC CCATGCATCG TATGCCCTAT 
    GTCACCTCTC CATACGACCG TCCAGCCTGG AACCCAAGAT ACTGTGTCCT CTCTGGAAAT
    CAGCTTCTCA TGCTTGATGA GGAGGAGGTT CACCCGCTGT TGATACGTGA AAGGAGAACT
    GATCCAACCA GAACAAAGTT CCTAAGGCGC ACAGTCAGTG TCCCCGTGGA GGGGAAGCAG
    CCTGACGAAC ACGATTATCA CCTTGCACGT TCCCGGAGGA AAAGCGTCCC AGGGGGAAAA
    CAGCCCAGTA TGGACGCCCC TCCCACCGCC CCCTTTCGAC CGTCGCAAGG ATTCCTGAGC
    CGTCGCTTGA AGAGCTCTAT AAAGAGGACA AAGAGTCAGC CCAAATTAGA TAGGACCAGC
    AGTTTCCGGC AAATATTGCC AAGGTTTCGA AGTGCTGATC ATGATAGGGC ACGGCTCATG
    CAGGCCTTTA AAGAGTCACA CTCTCACGAG TCCTTGCTTA GTCCTAGCAG TGCTGCAGAG
    GCTTTGGATT TGAACTTGGA TGAGGATTCT GTGGTGAAGC CTGTACACAG CAGCATCTTG
    GGACAGGAGT TTTGCTTCGA GGTGACCACA TCATCAGGTA CCAAATGTTT TGCCTGCCGT
    TCTGCTGCTG AAAGAGACAA ATGGATTGAG AATCTTCAAA GAGCTGTAAA GCCCAATAAG
    GATAACAGCA GGCGGGTAGA TAATGTCTTA AAGCTGTGGA TTATTGAAGC CCGTGATCTT
    CCCCCCAAGA AGCGCTATTA TTGTGAGCTC TGTTTGGATG ACATTTTGTA TGCCCGTACC
    ACCAGCAAGC CTCGGAATGA CACTGTCTTT TGGGGGGAAC ACTTTGAATT TAATAACCTT
    CCAGCTGTTC GGAACTTGCG CTTGCACCTC TATAAGGACA CAGACAAGAA AAGGCGCAAA
    GAAAAGAGCA ACTATGTGGG CATGGTGAAC GTGCCTATTG CCAGCATTAG TGGGCGCCAC
    TTTGTGGAGC AGTGGTACCC GGTTTCTCAG CCCACATCCT ACAAGGGGAA GAGCAGCTGT
    CCCTCCATTC GCCTAAAATC CCGATACCAA ACCATGAGCA TCCTCCCCAT GGAGCTGTAC
    AAGGAGTTTG CAGAGTATGT CACCAACAAC TACCGGATGC TGTGCGCTGT ACTGGAGCCT
    GTGCTTAGCG TGAAGAGCAA AGAGGAGGTT GCCTGTGCTT TAGTGCATAT ACTGCAAAGC
    ACAGGCAAGG CCAAGGACTT CCTTTCAGAC ATGGCTATGA CCGAGGTTGA TCGCTTCCTT
    GACAGAGAGC ACCTTATTCT CCGTGAGAAC ACACTTGCAA CTAAAGCCAT AGAGGAGTAT
    CTGAAACTGA TAGGACAGAG ATACCTCAAG GAAGCCATTG GTGAGTTTAT TCGGGCACTG
    TATGAGTCTG AGGAGGACTG TGAGGTTGAT CCCATGCGTT GTCCACCCTC TGCATTGCCC
    GATCACCAGG CCAATCTCAG GATGTGCTGT GAACTCGCCC TTTGCAAAGT CTTTAACTCT
    CATTGTGTGT TTCCTCGGGA GTTGAAGGAA GTCTTTGCAT CTTGGAGGAT GCGCTGTGCA
    GAGCGCGGCC GGGAAGATAT AGCAGATCGA CTCATCAGTG CCTCTCTCTT CCTCCGATTC
    CTGTGTCCGG CCATTATGTC CCCCAGTCTC TTCAGCCTAA TGCAGGAATA CCCTGATGAG
    CAAACATCTA GGACCCTCAC GCTCATCGCA AAAGTCATCC AGAACCTGGC CAACTTCAAC
    TACAAGTTCA GCAGTAAAGA AGAATATATG TCCTTTATGA ATGAGTTCCT AGAGACGGAG
    TTGGCGAATA TGCAGAATTT CCTGTATGAG ATCTCAAACC TGGATTCGCT CACCAACAGC
    ATTAACTTTG AGGGCTACAT TGACCTTGGG CGGGAGCTGA GTACACTTCA TGCCCTGCTT
    TGGGAAGTGA TCCCACAACT TAGTAAGGAT GCACTTATAA AGTTGGGGCC TCTTCCACGC
    CTCCTTAATG ACATCAGCCT AGCTCTCAGG AACCCCCACA TCCAACGACA ACCAAGCCAC
    CAAGAACGAC TGCCACCCCC CAAACCTGTC ATTCTGAGGG GACCTTCAGC TGAGCTGCAA
    AGCTACTTGG TGCGAGACCT CAACAGCTCT ATTGACATGA CTCGCCTACC CTCACCTACT
    AAGGATAAAC CATCAAAAGA TATGTTTTTT GTTTCACGTC GCCCGCTAAC TCGCTCCTCG
    CCAGCCTATT GCACCAGCAG CTCAGACATC ACAGAACCAG ACCAGAAGAT GCTGAGCGTG
    AACAAGAGTG TGTCCATGAT GGACTTGCAG GACAGCCGAA TGAATAGCGT AACCAACTTG
    CAGGCCAACC CGCTCTTCCA CATGGCTCCC GATGGGCCTG GTGGGCCCCC ATCTCATCCT
    GCTCCTCATC GACCAGACCC CGGAGCTGAT GCCTTTGCCC CTTTCCATGG GTATAGCAAG
    AGTGAGGACT TGAGCTCCGG AAAGCATATG CTTCACAGTC ACAGCTACAG CGACGAATTC
    AGCCGGCAGG GGTCAGAGTT CAGTAAAAGG CAGTTATCCC TCCAGGACAA TCTCCAGCAC
    ATGCTTTCAC CCCCTCAAAT AACCATTGGC CCTGGAGTGC CACGAAGGCA AGACTCACAG
    CCTCCCCCTT TGCAGAGGGG AAAATCCCAA CAACTGACGG TGCAGGCAGC GCATAAACCT
    CGGCCAAGCA GTGGCAACCT CCTACAGAGC CCAGAACCAA TGTATGGGGC AGCAAGAATT
    AGGCAGCAGT CAGTTGAAAA GGATGCAGGT GGCTTGAAAC CATCAATAAC CAAGCAGCAC
    TCCCATGCAC AGGCCACTCT GAACCCTGTC ACTCCAGCCT CAGAACGCAC AGTTGCCTGG
    GTATCCAACA TGCCCCACCT TTCAGCAGAT ATTGAGAGCG CTCACATAGA ACGGGAGGAG
    TATAAGTTGA AGGAATACTC TAAATCAATG GATGAGAGCC GTCTTGAGAG GGTGAAAGAG
    TATGAGGAAG AGATTTACTC CTTAAAAGAG AGGCTGTTGA TGTCAAACCG GAAGCTGGAG
    GAATATGAGA GGCGTCTTCT TACACAGGAA GAACAAACTA ATAAGATGAT GCAGCAATAT
    CAGTCACGCC TGGACCAGAG TGAAAAGCGT TTGCGACAGC AGCAGGTTGA GAAGGACACG
    CAGATAAAGA GCATCTTAAA CAGGTTAGAA ATGGTTGATG CTGGTGGAGG AGGAGATCAG
    GAAGGATCAC TCACAAGAAC CAATCGATAT GAAGAGGAGA CTTATCGAGG CTCAGAGAAG
    AAGTTTCCCT CCGTGGGTAC AGCAGACCCG CGTCTGAGGC TGGCGACTCC ATGGAATGGG
    GCCCTCCCGC CTCTGCCTCC TCGACTCCAA ATCACAGAAA ATGGAGACTT TCTAAACTCA
    AGTGACCATC ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa65735
    Clone ID Related Accession (Same CDS sequence) XM_031892382.1
    Accession Version XM_031892382.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3459bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ras/Rap GTPase-activating protein SynGAP isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGATGTTCT ATGCTCCCTT CAGAGATGCT CGAGGACCTC CCATGCATCG TATGCCCTAT 
    GTCACCTCTC CATACGACCG TCCAGCCTGG AACCCAAGAT ACTGTGTCCT CTCTGGAAAT
    CAGCTTCTCA TGCTTGATGA GGAGGAGGTT CACCCGCTGT TGATACGTGA AAGGAGAACT
    GATCCAACCA GAACAAAGTT CCTAAGGCGC ACAGTCAGTG TCCCCGTGGA GGGGAAGCAG
    CCTGACGAAC ACGATTATCA CCTTGCACGT TCCCGGAGGA AAAGCGTCCC AGGGGGAAAA
    CAGCCCAGTA TGGACGCCCC TCCCACCGCC CCCTTTCGAC CGTCGCAAGG ATTCCTGAGC
    CGTCGCTTGA AGAGCTCTAT AAAGAGGACA AAGAGTCAGC CCAAATTAGA TAGGACCAGC
    AGTTTCCGGC AAATATTGCC AAGGTTTCGA AGTGCTGATC ATGATAGGGC ACGGCTCATG
    CAGGCCTTTA AAGAGTCACA CTCTCACGAG TCCTTGCTTA GTCCTAGCAG TGCTGCAGAG
    GCTTTGGATT TGAACTTGGA TGAGGATTCT GTGGTGAAGC CTGTACACAG CAGCATCTTG
    GGACAGGAGT TTTGCTTCGA GGTGACCACA TCATCAGGTA CCAAATGTTT TGCCTGCCGT
    TCTGCTGCTG AAAGAGACAA ATGGATTGAG AATCTTCAAA GAGCTGTAAA GCCCAATAAG
    GATAACAGCA GGCGGGTAGA TAATGTCTTA AAGCTGTGGA TTATTGAAGC CCGTGATCTT
    CCCCCCAAGA AGCGCTATTA TTGTGAGCTC TGTTTGGATG ACATTTTGTA TGCCCGTACC
    ACCAGCAAGC CTCGGAATGA CACTGTCTTT TGGGGGGAAC ACTTTGAATT TAATAACCTT
    CCAGCTGTTC GGAACTTGCG CTTGCACCTC TATAAGGACA CAGACAAGAA AAGGCGCAAA
    GAAAAGAGCA ACTATGTGGG CATGGTGAAC GTGCCTATTG CCAGCATTAG TGGGCGCCAC
    TTTGTGGAGC AGTGGTACCC GGTTTCTCAG CCCACATCCT ACAAGGGGAA GAGCAGCTGT
    CCCTCCATTC GCCTAAAATC CCGATACCAA ACCATGAGCA TCCTCCCCAT GGAGCTGTAC
    AAGGAGTTTG CAGAGTATGT CACCAACAAC TACCGGATGC TGTGCGCTGT ACTGGAGCCT
    GTGCTTAGCG TGAAGAGCAA AGAGGAGGTT GCCTGTGCTT TAGTGCATAT ACTGCAAAGC
    ACAGGCAAGG CCAAGGACTT CCTTTCAGAC ATGGCTATGA CCGAGGTTGA TCGCTTCCTT
    GACAGAGAGC ACCTTATTCT CCGTGAGAAC ACACTTGCAA CTAAAGCCAT AGAGGAGTAT
    CTGAAACTGA TAGGACAGAG ATACCTCAAG GAAGCCATTG GTGAGTTTAT TCGGGCACTG
    TATGAGTCTG AGGAGGACTG TGAGGTTGAT CCCATGCGTT GTCCACCCTC TGCATTGCCC
    GATCACCAGG CCAATCTCAG GATGTGCTGT GAACTCGCCC TTTGCAAAGT CTTTAACTCT
    CATTGTGTGT TTCCTCGGGA GTTGAAGGAA GTCTTTGCAT CTTGGAGGAT GCGCTGTGCA
    GAGCGCGGCC GGGAAGATAT AGCAGATCGA CTCATCAGTG CCTCTCTCTT CCTCCGATTC
    CTGTGTCCGG CCATTATGTC CCCCAGTCTC TTCAGCCTAA TGCAGGAATA CCCTGATGAG
    CAAACATCTA GGACCCTCAC GCTCATCGCA AAAGTCATCC AGAACCTGGC CAACTTCAAC
    TACAACAGGT TCAGCAGTAA AGAAGAATAT ATGTCCTTTA TGAATGAGTT CCTAGAGACG
    GAGTTGGCGA ATATGCAGAA TTTCCTGTAT GAGATCTCAA ACCTGGATTC GCTCACCAAC
    AGCATTAACT TTGAGGGCTA CATTGACCTT GGGCGGGAGC TGAGTACACT TCATGCCCTG
    CTTTGGGAAG TGATCCCACA ACTTAGTAAG GATGCACTTA TAAAGTTGGG GCCTCTTCCA
    CGCCTCCTTA ATGACATCAG CCTAGCTCTC AGGAACCCCC ACATCCAACG ACAACCAAGC
    CACCAAGAAC GACTGCCACC CCCCAAACCT GTCATTCTGA GGGGACCTTC AGCTGAGCTG
    CAAAGCTACT TGGTGCGAGA CCTCAACAGC TCTATTGACA TGACTCGCCT ACCCTCACCT
    ACTAAGGATA AACCATCAAA AGATATGTTT TTTGTTTCAC GTCGCCCGCT AACTCGCTCC
    TCGCCAGCCT ATTGCACCAG CAGCTCAGAC ATCACAGAAC CAGACCAGAA GATGCTGAGC
    GTGAACAAGA GTGTGTCCAT GATGGACTTG CAGGACAGCC GAATGAATAG CGTAACCAAC
    TTGCAGGCCG TAGGTGATCT GCTGAACTCC AGCCAGGCCT CCATATCAGG GGGCCTACGT
    CCGGGGGGCC GGCTCTCCCA GGGCAGTGGA TCCAGCATTG CAGCGGGTCT GAGGCTCAGT
    CAGATGGGGG TCACCACTGA TGGGCTCTCA GCTCAACAGC TACGCATTCC CTTGTCCTTC
    CAGAACCCGC TCTTCCACAT GGCTCCCGAT GGGCCTGGTG GGCCCCCATC TCATCCTGCT
    CCTCATCGAC CAGACCCCGG AGCTGATGCC TTTGCCCCTT TCCATGGGTA TAGCAAGAGT
    GAGGACTTGA GCTCCGGAAA GCATATGCTT CACAGTCACA GCTACAGCGA CGAATTCAGC
    CGGCAGGGGT CAGAGTTCAG TAAAAGGCAG TTATCCCTCC AGGACAATCT CCAGCACATG
    CTTTCACCCC CTCAAATAAC CATTGGCCCT GGAGTGCCAC GAAGGCAAGA CTCACAGCCT
    CCCCCTTTGC AGAGGGGAAA ATCCCAACAA CTGACGGTGC AGGCAGCGCA TAAACCTCGG
    CCAAGCAGTG GCAACCTCCT ACAGAGCCCA GAACCAATGT ATGGGGCAGC AAGAATTAGG
    CAGCAGTCAG TTGAAAAGGA TGCAGGTGGC TTGAAACCAT CAATAACCAA GCAGCACTCC
    CATGCACAGG CCACTCTGAA CCCTGTCACT CCAGCCTCAG AACGCACAGT TGCCTGGGTA
    TCCAACATGC CCCACCTTTC AGCAGATATT GAGAGCGCTC ACATAGAACG GGAGGAGTAT
    AAGTTGAAGG AATACTCTAA ATCAATGGAT GAGAGCCGTC TTGAGAGGGT GAAAGAGTAT
    GAGGAAGAGA TTTACTCCTT AAAAGAGAGG CTGTTGATGT CAAACCGGAA GCTGGAGGAA
    TATGAGAGGC GTCTTCTTAC ACAGGAAGAA CAAACTAATA AGATGATGCA GCAATATCAG
    TCACGCCTGG ACCAGAGTGA AAAGCGTTTG CGACAGCAGC AGGTTGAGAA GGACACGCAG
    ATAAAGAGCA TCTTAAACAG GTTAGAAATG GTAAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031748242.1
    CDS665..4123
    Translation

    Target ORF information:

    RefSeq Version XM_031892382.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031892382.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGATGTTCT ATGCTCCCTT CAGAGATGCT CGAGGACCTC CCATGCATCG TATGCCCTAT 
    GTCACCTCTC CATACGACCG TCCAGCCTGG AACCCAAGAT ACTGTGTCCT CTCTGGAAAT
    CAGCTTCTCA TGCTTGATGA GGAGGAGGTT CACCCGCTGT TGATACGTGA AAGGAGAACT
    GATCCAACCA GAACAAAGTT CCTAAGGCGC ACAGTCAGTG TCCCCGTGGA GGGGAAGCAG
    CCTGACGAAC ACGATTATCA CCTTGCACGT TCCCGGAGGA AAAGCGTCCC AGGGGGAAAA
    CAGCCCAGTA TGGACGCCCC TCCCACCGCC CCCTTTCGAC CGTCGCAAGG ATTCCTGAGC
    CGTCGCTTGA AGAGCTCTAT AAAGAGGACA AAGAGTCAGC CCAAATTAGA TAGGACCAGC
    AGTTTCCGGC AAATATTGCC AAGGTTTCGA AGTGCTGATC ATGATAGGGC ACGGCTCATG
    CAGGCCTTTA AAGAGTCACA CTCTCACGAG TCCTTGCTTA GTCCTAGCAG TGCTGCAGAG
    GCTTTGGATT TGAACTTGGA TGAGGATTCT GTGGTGAAGC CTGTACACAG CAGCATCTTG
    GGACAGGAGT TTTGCTTCGA GGTGACCACA TCATCAGGTA CCAAATGTTT TGCCTGCCGT
    TCTGCTGCTG AAAGAGACAA ATGGATTGAG AATCTTCAAA GAGCTGTAAA GCCCAATAAG
    GATAACAGCA GGCGGGTAGA TAATGTCTTA AAGCTGTGGA TTATTGAAGC CCGTGATCTT
    CCCCCCAAGA AGCGCTATTA TTGTGAGCTC TGTTTGGATG ACATTTTGTA TGCCCGTACC
    ACCAGCAAGC CTCGGAATGA CACTGTCTTT TGGGGGGAAC ACTTTGAATT TAATAACCTT
    CCAGCTGTTC GGAACTTGCG CTTGCACCTC TATAAGGACA CAGACAAGAA AAGGCGCAAA
    GAAAAGAGCA ACTATGTGGG CATGGTGAAC GTGCCTATTG CCAGCATTAG TGGGCGCCAC
    TTTGTGGAGC AGTGGTACCC GGTTTCTCAG CCCACATCCT ACAAGGGGAA GAGCAGCTGT
    CCCTCCATTC GCCTAAAATC CCGATACCAA ACCATGAGCA TCCTCCCCAT GGAGCTGTAC
    AAGGAGTTTG CAGAGTATGT CACCAACAAC TACCGGATGC TGTGCGCTGT ACTGGAGCCT
    GTGCTTAGCG TGAAGAGCAA AGAGGAGGTT GCCTGTGCTT TAGTGCATAT ACTGCAAAGC
    ACAGGCAAGG CCAAGGACTT CCTTTCAGAC ATGGCTATGA CCGAGGTTGA TCGCTTCCTT
    GACAGAGAGC ACCTTATTCT CCGTGAGAAC ACACTTGCAA CTAAAGCCAT AGAGGAGTAT
    CTGAAACTGA TAGGACAGAG ATACCTCAAG GAAGCCATTG GTGAGTTTAT TCGGGCACTG
    TATGAGTCTG AGGAGGACTG TGAGGTTGAT CCCATGCGTT GTCCACCCTC TGCATTGCCC
    GATCACCAGG CCAATCTCAG GATGTGCTGT GAACTCGCCC TTTGCAAAGT CTTTAACTCT
    CATTGTGTGT TTCCTCGGGA GTTGAAGGAA GTCTTTGCAT CTTGGAGGAT GCGCTGTGCA
    GAGCGCGGCC GGGAAGATAT AGCAGATCGA CTCATCAGTG CCTCTCTCTT CCTCCGATTC
    CTGTGTCCGG CCATTATGTC CCCCAGTCTC TTCAGCCTAA TGCAGGAATA CCCTGATGAG
    CAAACATCTA GGACCCTCAC GCTCATCGCA AAAGTCATCC AGAACCTGGC CAACTTCAAC
    TACAACAGGT TCAGCAGTAA AGAAGAATAT ATGTCCTTTA TGAATGAGTT CCTAGAGACG
    GAGTTGGCGA ATATGCAGAA TTTCCTGTAT GAGATCTCAA ACCTGGATTC GCTCACCAAC
    AGCATTAACT TTGAGGGCTA CATTGACCTT GGGCGGGAGC TGAGTACACT TCATGCCCTG
    CTTTGGGAAG TGATCCCACA ACTTAGTAAG GATGCACTTA TAAAGTTGGG GCCTCTTCCA
    CGCCTCCTTA ATGACATCAG CCTAGCTCTC AGGAACCCCC ACATCCAACG ACAACCAAGC
    CACCAAGAAC GACTGCCACC CCCCAAACCT GTCATTCTGA GGGGACCTTC AGCTGAGCTG
    CAAAGCTACT TGGTGCGAGA CCTCAACAGC TCTATTGACA TGACTCGCCT ACCCTCACCT
    ACTAAGGATA AACCATCAAA AGATATGTTT TTTGTTTCAC GTCGCCCGCT AACTCGCTCC
    TCGCCAGCCT ATTGCACCAG CAGCTCAGAC ATCACAGAAC CAGACCAGAA GATGCTGAGC
    GTGAACAAGA GTGTGTCCAT GATGGACTTG CAGGACAGCC GAATGAATAG CGTAACCAAC
    TTGCAGGCCG TAGGTGATCT GCTGAACTCC AGCCAGGCCT CCATATCAGG GGGCCTACGT
    CCGGGGGGCC GGCTCTCCCA GGGCAGTGGA TCCAGCATTG CAGCGGGTCT GAGGCTCAGT
    CAGATGGGGG TCACCACTGA TGGGCTCTCA GCTCAACAGC TACGCATTCC CTTGTCCTTC
    CAGAACCCGC TCTTCCACAT GGCTCCCGAT GGGCCTGGTG GGCCCCCATC TCATCCTGCT
    CCTCATCGAC CAGACCCCGG AGCTGATGCC TTTGCCCCTT TCCATGGGTA TAGCAAGAGT
    GAGGACTTGA GCTCCGGAAA GCATATGCTT CACAGTCACA GCTACAGCGA CGAATTCAGC
    CGGCAGGGGT CAGAGTTCAG TAAAAGGCAG TTATCCCTCC AGGACAATCT CCAGCACATG
    CTTTCACCCC CTCAAATAAC CATTGGCCCT GGAGTGCCAC GAAGGCAAGA CTCACAGCCT
    CCCCCTTTGC AGAGGGGAAA ATCCCAACAA CTGACGGTGC AGGCAGCGCA TAAACCTCGG
    CCAAGCAGTG GCAACCTCCT ACAGAGCCCA GAACCAATGT ATGGGGCAGC AAGAATTAGG
    CAGCAGTCAG TTGAAAAGGA TGCAGGTGGC TTGAAACCAT CAATAACCAA GCAGCACTCC
    CATGCACAGG CCACTCTGAA CCCTGTCACT CCAGCCTCAG AACGCACAGT TGCCTGGGTA
    TCCAACATGC CCCACCTTTC AGCAGATATT GAGAGCGCTC ACATAGAACG GGAGGAGTAT
    AAGTTGAAGG AATACTCTAA ATCAATGGAT GAGAGCCGTC TTGAGAGGGT GAAAGAGTAT
    GAGGAAGAGA TTTACTCCTT AAAAGAGAGG CTGTTGATGT CAAACCGGAA GCTGGAGGAA
    TATGAGAGGC GTCTTCTTAC ACAGGAAGAA CAAACTAATA AGATGATGCA GCAATATCAG
    TCACGCCTGG ACCAGAGTGA AAAGCGTTTG CGACAGCAGC AGGTTGAGAA GGACACGCAG
    ATAAAGAGCA TCTTAAACAG GTTAGAAATG GTAAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa65736
    Clone ID Related Accession (Same CDS sequence) XM_031892383.1
    Accession Version XM_031892383.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3444bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ras/Rap GTPase-activating protein SynGAP isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGATGTTCT ATGCTCCCTT CAGAGATGCT CGAGGACCTC CCATGCATCG TATGCCCTAT 
    GTCACCTCTC CATACGACCG TCCAGCCTGG AACCCAAGAT ACTGTGTCCT CTCTGGAAAT
    CAGCTTCTCA TGCTTGATGA GGAGGAGGTT CACCCGCTGT TGATACGTGA AAGGAGAACT
    GATCCAACCA GAACAAAGTT CCTAAGGCGC ACAGTCAGTG TCCCCGTGGA GGGGAAGCAG
    CCTGACGAAC ACGATTATCA CCTTGCACGT TCCCGGAGGA AAAGCGTCCC AGGGGGAAAA
    CAGCCCAGTA TGGACGCCCC TCCCACCGCC CCCTTTCGAC CGTCGCAAGG ATTCCTGAGC
    CGTCGCTTGA AGAGCTCTAT AAAGAGGACA AAGAGTCAGC CCAAATTAGA TAGGACCAGC
    AGTTTCCGGC AAATATTGCC AAGGTTTCGA AGTGCTGATC ATGATAGGGC ACGGCTCATG
    CAGGCCTTTA AAGAGTCACA CTCTCACGAG TCCTTGCTTA GTCCTAGCAG TGCTGCAGAG
    GCTTTGGATT TGAACTTGGA TGAGGATTCT GTGGTGAAGC CTGTACACAG CAGCATCTTG
    GGACAGGAGT TTTGCTTCGA GGTGACCACA TCATCAGGTA CCAAATGTTT TGCCTGCCGT
    TCTGCTGCTG AAAGAGACAA ATGGATTGAG AATCTTCAAA GAGCTGTAAA GCCCAATAAG
    GATAACAGCA GGCGGGTAGA TAATGTCTTA AAGCTGTGGA TTATTGAAGC CCGTGATCTT
    CCCCCCAAGA AGCGCTATTA TTGTGAGCTC TGTTTGGATG ACATTTTGTA TGCCCGTACC
    ACCAGCAAGC CTCGGAATGA CACTGTCTTT TGGGGGGAAC ACTTTGAATT TAATAACCTT
    CCAGCTGTTC GGAACTTGCG CTTGCACCTC TATAAGGACA CAGACAAGAA AAGGCGCAAA
    GAAAAGAGCA ACTATGTGGG CATGGTGAAC GTGCCTATTG CCAGCATTAG TGGGCGCCAC
    TTTGTGGAGC AGTGGTACCC GGTTTCTCAG CCCACATCCT ACAAGGGGAA GAGCAGCTGT
    CCCTCCATTC GCCTAAAATC CCGATACCAA ACCATGAGCA TCCTCCCCAT GGAGCTGTAC
    AAGGAGTTTG CAGAGTATGT CACCAACAAC TACCGGATGC TGTGCGCTGT ACTGGAGCCT
    GTGCTTAGCG TGAAGAGCAA AGAGGAGGTT GCCTGTGCTT TAGTGCATAT ACTGCAAAGC
    ACAGGCAAGG CCAAGGACTT CCTTTCAGAC ATGGCTATGA CCGAGGTTGA TCGCTTCCTT
    GACAGAGAGC ACCTTATTCT CCGTGAGAAC ACACTTGCAA CTAAAGCCAT AGAGGAGTAT
    CTGAAACTGA TAGGACAGAG ATACCTCAAG GAAGCCATTG GTGAGTTTAT TCGGGCACTG
    TATGAGTCTG AGGAGGACTG TGAGGTTGAT CCCATGCGTT GTCCACCCTC TGCATTGCCC
    GATCACCAGG CCAATCTCAG GATGTGCTGT GAACTCGCCC TTTGCAAAGT CTTTAACTCT
    CATTGTGTGT TTCCTCGGGA GTTGAAGGAA GTCTTTGCAT CTTGGAGGAT GCGCTGTGCA
    GAGCGCGGCC GGGAAGATAT AGCAGATCGA CTCATCAGTG CCTCTCTCTT CCTCCGATTC
    CTGTGTCCGG CCATTATGTC CCCCAGTCTC TTCAGCCTAA TGCAGGAATA CCCTGATGAG
    CAAACATCTA GGACCCTCAC GCTCATCGCA AAAGTCATCC AGAACCTGGC CAACTTCAAC
    TACAACAGGT TCAGCAGTAA AGAAGAATAT ATGTCCTTTA TGAATGAGTT CCTAGAGACG
    GAGTTGGCGA ATATGCAGAA TTTCCTGTAT GAGATCTCAA ACCTGGATTC GCTCACCAAC
    AGCATTAACT TTGAGGGCTA CATTGACCTT GGGCGGGAGC TGAGTACACT TCATGCCCTG
    CTTTGGGAAG TGATCCCACA ACTTAGTAAG GATGCACTTA TAAAGTTGGG GCCTCTTCCA
    CGCCTCCTTA ATGACATCAG CCTAGCTCTC AGGAACCCCC ACATCCAACG ACAACCAAGC
    CACCAAGAAC GACTGCCACC CCCCAAACCT GTCATTCTGA GGGGACCTTC AGCTGAGCTG
    CAAAGCTACT TGGTGCGAGA CCTCAACAGC TCTATTGACA TGACTCGCCT ACCCTCACCT
    ACTAAGGATA AACCATCAAA AGATATGTTT TTTGTTTCAC GTCGCCCGCT AACTCGCTCC
    TCGCCAGCCT ATTGCACCAG CAGCTCAGAC ATCACAGAAC CAGACCAGAA GATGCTGAGC
    GTGAACAAGA GTGTGTCCAT GATGGACTTG CAGGACAGCC GAATGAATAG CGTAACCAAC
    TTGCAGGCCG TAGGTGATCT GCTGAACTCC AGCCAGGCCT CCATATCAGG GGGCCTACGT
    CCGGGGGGCC GGCTCTCCCA GGGCAGTGGA TCCAGCATTG CAGCGGGTCT GAGGCTCAGT
    CAGATGGGGG TCACCACTGA TGGGCTCTCA GCTCAACAGC TACGCATTCC CTTGTCCTTC
    CAGAACCCGC TCTTCCACAT GGCTCCCGAT GGGCCTGGTG GGCCCCCATC TCATCCTGCT
    CCTCATCGAC CAGACCCCGG AGCTGATGCC TTTGCCCCTT TCCATGGGTA TAGCAAGAGT
    GAGGACTTGA GCTCCGGAAA GCATATGCTT CACAGTCACA GCTACAGCGA CGAATTCAGC
    CGGCAGGGGT CAGAGTTCAG TAAAAGGCAG TTATCCCTCC AGGACAATCT CCAGCACATG
    CTTTCACCCC CTCAAATAAC CATTGGCCCT GGAGTGCCAC GAAGGCAAGA CTCACAGCCT
    CCCCCTTTGC AGAGGGGAAA ATCCCAACAA CTGACGGTGC AGGCAGCGCA TAAACCTCGG
    CCAAGCAGTG GCAACCTCCT ACAGAGCCCA GAACCAATGT ATGGGGCAGC AAGAATTAGG
    CAGCAGTCAG TTGAAAAGGA TGCAGGTGGC TTGAAACCAT CAATAACCAA GCAGCACTCC
    CATGCACAGG CCACTCTGAA CCCTGTCACT CCAGCCTCAG AACGCACAGT TGCCTGGGTA
    TCCAACATGC CCCACCTTTC AGCAGATATT GAGAGCGCTC ACATAGAACG GGAGGAGTAT
    AAGTTGAAGG AATACTCTAA ATCAATGGAT GAGAGCCGTC TTGAGAGGGT GAAAGAGTAT
    GAGGAAGAGA TTTACTCCTT AAAAGAGAGG CTGTTGATGT CAAACCGGAA GCTGGAGGAA
    TATGAGAGGC GTCTTCTTAC ACAGGAAGAA CAAACTAATA AGATGATGCA GCAATATCAG
    TCACGCCTGG ACCAGAGTGA AAAGCGTTTG CGACAGCAGC AGGTTGAGAA GGACACGCAG
    ATAAAGAGCA TCTTAAACAG GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031748243.1
    CDS665..4108
    Translation

    Target ORF information:

    RefSeq Version XM_031892383.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031892383.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGATGTTCT ATGCTCCCTT CAGAGATGCT CGAGGACCTC CCATGCATCG TATGCCCTAT 
    GTCACCTCTC CATACGACCG TCCAGCCTGG AACCCAAGAT ACTGTGTCCT CTCTGGAAAT
    CAGCTTCTCA TGCTTGATGA GGAGGAGGTT CACCCGCTGT TGATACGTGA AAGGAGAACT
    GATCCAACCA GAACAAAGTT CCTAAGGCGC ACAGTCAGTG TCCCCGTGGA GGGGAAGCAG
    CCTGACGAAC ACGATTATCA CCTTGCACGT TCCCGGAGGA AAAGCGTCCC AGGGGGAAAA
    CAGCCCAGTA TGGACGCCCC TCCCACCGCC CCCTTTCGAC CGTCGCAAGG ATTCCTGAGC
    CGTCGCTTGA AGAGCTCTAT AAAGAGGACA AAGAGTCAGC CCAAATTAGA TAGGACCAGC
    AGTTTCCGGC AAATATTGCC AAGGTTTCGA AGTGCTGATC ATGATAGGGC ACGGCTCATG
    CAGGCCTTTA AAGAGTCACA CTCTCACGAG TCCTTGCTTA GTCCTAGCAG TGCTGCAGAG
    GCTTTGGATT TGAACTTGGA TGAGGATTCT GTGGTGAAGC CTGTACACAG CAGCATCTTG
    GGACAGGAGT TTTGCTTCGA GGTGACCACA TCATCAGGTA CCAAATGTTT TGCCTGCCGT
    TCTGCTGCTG AAAGAGACAA ATGGATTGAG AATCTTCAAA GAGCTGTAAA GCCCAATAAG
    GATAACAGCA GGCGGGTAGA TAATGTCTTA AAGCTGTGGA TTATTGAAGC CCGTGATCTT
    CCCCCCAAGA AGCGCTATTA TTGTGAGCTC TGTTTGGATG ACATTTTGTA TGCCCGTACC
    ACCAGCAAGC CTCGGAATGA CACTGTCTTT TGGGGGGAAC ACTTTGAATT TAATAACCTT
    CCAGCTGTTC GGAACTTGCG CTTGCACCTC TATAAGGACA CAGACAAGAA AAGGCGCAAA
    GAAAAGAGCA ACTATGTGGG CATGGTGAAC GTGCCTATTG CCAGCATTAG TGGGCGCCAC
    TTTGTGGAGC AGTGGTACCC GGTTTCTCAG CCCACATCCT ACAAGGGGAA GAGCAGCTGT
    CCCTCCATTC GCCTAAAATC CCGATACCAA ACCATGAGCA TCCTCCCCAT GGAGCTGTAC
    AAGGAGTTTG CAGAGTATGT CACCAACAAC TACCGGATGC TGTGCGCTGT ACTGGAGCCT
    GTGCTTAGCG TGAAGAGCAA AGAGGAGGTT GCCTGTGCTT TAGTGCATAT ACTGCAAAGC
    ACAGGCAAGG CCAAGGACTT CCTTTCAGAC ATGGCTATGA CCGAGGTTGA TCGCTTCCTT
    GACAGAGAGC ACCTTATTCT CCGTGAGAAC ACACTTGCAA CTAAAGCCAT AGAGGAGTAT
    CTGAAACTGA TAGGACAGAG ATACCTCAAG GAAGCCATTG GTGAGTTTAT TCGGGCACTG
    TATGAGTCTG AGGAGGACTG TGAGGTTGAT CCCATGCGTT GTCCACCCTC TGCATTGCCC
    GATCACCAGG CCAATCTCAG GATGTGCTGT GAACTCGCCC TTTGCAAAGT CTTTAACTCT
    CATTGTGTGT TTCCTCGGGA GTTGAAGGAA GTCTTTGCAT CTTGGAGGAT GCGCTGTGCA
    GAGCGCGGCC GGGAAGATAT AGCAGATCGA CTCATCAGTG CCTCTCTCTT CCTCCGATTC
    CTGTGTCCGG CCATTATGTC CCCCAGTCTC TTCAGCCTAA TGCAGGAATA CCCTGATGAG
    CAAACATCTA GGACCCTCAC GCTCATCGCA AAAGTCATCC AGAACCTGGC CAACTTCAAC
    TACAACAGGT TCAGCAGTAA AGAAGAATAT ATGTCCTTTA TGAATGAGTT CCTAGAGACG
    GAGTTGGCGA ATATGCAGAA TTTCCTGTAT GAGATCTCAA ACCTGGATTC GCTCACCAAC
    AGCATTAACT TTGAGGGCTA CATTGACCTT GGGCGGGAGC TGAGTACACT TCATGCCCTG
    CTTTGGGAAG TGATCCCACA ACTTAGTAAG GATGCACTTA TAAAGTTGGG GCCTCTTCCA
    CGCCTCCTTA ATGACATCAG CCTAGCTCTC AGGAACCCCC ACATCCAACG ACAACCAAGC
    CACCAAGAAC GACTGCCACC CCCCAAACCT GTCATTCTGA GGGGACCTTC AGCTGAGCTG
    CAAAGCTACT TGGTGCGAGA CCTCAACAGC TCTATTGACA TGACTCGCCT ACCCTCACCT
    ACTAAGGATA AACCATCAAA AGATATGTTT TTTGTTTCAC GTCGCCCGCT AACTCGCTCC
    TCGCCAGCCT ATTGCACCAG CAGCTCAGAC ATCACAGAAC CAGACCAGAA GATGCTGAGC
    GTGAACAAGA GTGTGTCCAT GATGGACTTG CAGGACAGCC GAATGAATAG CGTAACCAAC
    TTGCAGGCCG TAGGTGATCT GCTGAACTCC AGCCAGGCCT CCATATCAGG GGGCCTACGT
    CCGGGGGGCC GGCTCTCCCA GGGCAGTGGA TCCAGCATTG CAGCGGGTCT GAGGCTCAGT
    CAGATGGGGG TCACCACTGA TGGGCTCTCA GCTCAACAGC TACGCATTCC CTTGTCCTTC
    CAGAACCCGC TCTTCCACAT GGCTCCCGAT GGGCCTGGTG GGCCCCCATC TCATCCTGCT
    CCTCATCGAC CAGACCCCGG AGCTGATGCC TTTGCCCCTT TCCATGGGTA TAGCAAGAGT
    GAGGACTTGA GCTCCGGAAA GCATATGCTT CACAGTCACA GCTACAGCGA CGAATTCAGC
    CGGCAGGGGT CAGAGTTCAG TAAAAGGCAG TTATCCCTCC AGGACAATCT CCAGCACATG
    CTTTCACCCC CTCAAATAAC CATTGGCCCT GGAGTGCCAC GAAGGCAAGA CTCACAGCCT
    CCCCCTTTGC AGAGGGGAAA ATCCCAACAA CTGACGGTGC AGGCAGCGCA TAAACCTCGG
    CCAAGCAGTG GCAACCTCCT ACAGAGCCCA GAACCAATGT ATGGGGCAGC AAGAATTAGG
    CAGCAGTCAG TTGAAAAGGA TGCAGGTGGC TTGAAACCAT CAATAACCAA GCAGCACTCC
    CATGCACAGG CCACTCTGAA CCCTGTCACT CCAGCCTCAG AACGCACAGT TGCCTGGGTA
    TCCAACATGC CCCACCTTTC AGCAGATATT GAGAGCGCTC ACATAGAACG GGAGGAGTAT
    AAGTTGAAGG AATACTCTAA ATCAATGGAT GAGAGCCGTC TTGAGAGGGT GAAAGAGTAT
    GAGGAAGAGA TTTACTCCTT AAAAGAGAGG CTGTTGATGT CAAACCGGAA GCTGGAGGAA
    TATGAGAGGC GTCTTCTTAC ACAGGAAGAA CAAACTAATA AGATGATGCA GCAATATCAG
    TCACGCCTGG ACCAGAGTGA AAAGCGTTTG CGACAGCAGC AGGTTGAGAA GGACACGCAG
    ATAAAGAGCA TCTTAAACAG GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa65737
    Clone ID Related Accession (Same CDS sequence) XM_031892384.1
    Accession Version XM_031892384.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3423bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ras/Rap GTPase-activating protein SynGAP isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGATTATC ACCTTGCACG TTCCCGGAGG AAAAGCGTCC CAGGGGGAAA ACAGCCCAGT 
    ATGGACGCCC CTCCCACCGC CCCCTTTCGA CCGTCGCAAG GATTCCTGAG CCGTCGCTTG
    AAGAGCTCTA TAAAGAGGAC AAAGAGTCAG CCCAAATTAG ATAGGACCAG CAGTTTCCGG
    CAAATATTGC CAAGGTTTCG AAGTGCTGAT CATGATAGGG CACGGCTCAT GCAGGCCTTT
    AAAGAGTCAC ACTCTCACGA GTCCTTGCTT AGTCCTAGCA GTGCTGCAGA GGCTTTGGAT
    TTGAACTTGG ATGAGGATTC TGTGGTGAAG CCTGTACACA GCAGCATCTT GGGACAGGAG
    TTTTGCTTCG AGGTGACCAC ATCATCAGGT ACCAAATGTT TTGCCTGCCG TTCTGCTGCT
    GAAAGAGACA AATGGATTGA GAATCTTCAA AGAGCTGTAA AGCCCAATAA GGATAACAGC
    AGGCGGGTAG ATAATGTCTT AAAGCTGTGG ATTATTGAAG CCCGTGATCT TCCCCCCAAG
    AAGCGCTATT ATTGTGAGCT CTGTTTGGAT GACATTTTGT ATGCCCGTAC CACCAGCAAG
    CCTCGGAATG ACACTGTCTT TTGGGGGGAA CACTTTGAAT TTAATAACCT TCCAGCTGTT
    CGGAACTTGC GCTTGCACCT CTATAAGGAC ACAGACAAGA AAAGGCGCAA AGAAAAGAGC
    AACTATGTGG GCATGGTGAA CGTGCCTATT GCCAGCATTA GTGGGCGCCA CTTTGTGGAG
    CAGTGGTACC CGGTTTCTCA GCCCACATCC TACAAGGGGA AGAGCAGCTG TCCCTCCATT
    CGCCTAAAAT CCCGATACCA AACCATGAGC ATCCTCCCCA TGGAGCTGTA CAAGGAGTTT
    GCAGAGTATG TCACCAACAA CTACCGGATG CTGTGCGCTG TACTGGAGCC TGTGCTTAGC
    GTGAAGAGCA AAGAGGAGGT TGCCTGTGCT TTAGTGCATA TACTGCAAAG CACAGGCAAG
    GCCAAGGACT TCCTTTCAGA CATGGCTATG ACCGAGGTTG ATCGCTTCCT TGACAGAGAG
    CACCTTATTC TCCGTGAGAA CACACTTGCA ACTAAAGCCA TAGAGGAGTA TCTGAAACTG
    ATAGGACAGA GATACCTCAA GGAAGCCATT GGTGAGTTTA TTCGGGCACT GTATGAGTCT
    GAGGAGGACT GTGAGGTTGA TCCCATGCGT TGTCCACCCT CTGCATTGCC CGATCACCAG
    GCCAATCTCA GGATGTGCTG TGAACTCGCC CTTTGCAAAG TCTTTAACTC TCATTGTGTG
    TTTCCTCGGG AGTTGAAGGA AGTCTTTGCA TCTTGGAGGA TGCGCTGTGC AGAGCGCGGC
    CGGGAAGATA TAGCAGATCG ACTCATCAGT GCCTCTCTCT TCCTCCGATT CCTGTGTCCG
    GCCATTATGT CCCCCAGTCT CTTCAGCCTA ATGCAGGAAT ACCCTGATGA GCAAACATCT
    AGGACCCTCA CGCTCATCGC AAAAGTCATC CAGAACCTGG CCAACTTCAA CTACAACAGG
    TTCAGCAGTA AAGAAGAATA TATGTCCTTT ATGAATGAGT TCCTAGAGAC GGAGTTGGCG
    AATATGCAGA ATTTCCTGTA TGAGATCTCA AACCTGGATT CGCTCACCAA CAGCATTAAC
    TTTGAGGGCT ACATTGACCT TGGGCGGGAG CTGAGTACAC TTCATGCCCT GCTTTGGGAA
    GTGATCCCAC AACTTAGTAA GGATGCACTT ATAAAGTTGG GGCCTCTTCC ACGCCTCCTT
    AATGACATCA GCCTAGCTCT CAGGAACCCC CACATCCAAC GACAACCAAG CCACCAAGAA
    CGACTGCCAC CCCCCAAACC TGTCATTCTG AGGGGACCTT CAGCTGAGCT GCAAAGCTAC
    TTGGTGCGAG ACCTCAACAG CTCTATTGAC ATGACTCGCC TACCCTCACC TACTAAGGAT
    AAACCATCAA AAGATATGTT TTTTGTTTCA CGTCGCCCGC TAACTCGCTC CTCGCCAGCC
    TATTGCACCA GCAGCTCAGA CATCACAGAA CCAGACCAGA AGATGCTGAG CGTGAACAAG
    AGTGTGTCCA TGATGGACTT GCAGGACAGC CGAATGAATA GCGTAACCAA CTTGCAGGCC
    GTAGGTGATC TGCTGAACTC CAGCCAGGCC TCCATATCAG GGGGCCTACG TCCGGGGGGC
    CGGCTCTCCC AGGGCAGTGG ATCCAGCATT GCAGCGGGTC TGAGGCTCAG TCAGATGGGG
    GTCACCACTG ATGGGCTCTC AGCTCAACAG CTACGCATTC CCTTGTCCTT CCAGAACCCG
    CTCTTCCACA TGGCTCCCGA TGGGCCTGGT GGGCCCCCAT CTCATCCTGC TCCTCATCGA
    CCAGACCCCG GAGCTGATGC CTTTGCCCCT TTCCATGGGT ATAGCAAGAG TGAGGACTTG
    AGCTCCGGAA AGCATATGCT TCACAGTCAC AGCTACAGCG ACGAATTCAG CCGGCAGGGG
    TCAGAGTTCA GTAAAAGGCA GTTATCCCTC CAGGACAATC TCCAGCACAT GCTTTCACCC
    CCTCAAATAA CCATTGGCCC TGGAGTGCCA CGAAGGCAAG ACTCACAGCC TCCCCCTTTG
    CAGAGGGGAA AATCCCAACA ACTGACGGTG CAGGCAGCGC ATAAACCTCG GCCAAGCAGT
    GGCAACCTCC TACAGAGCCC AGAACCAATG TATGGGGCAG CAAGAATTAG GCAGCAGTCA
    GTTGAAAAGG ATGCAGGTGG CTTGAAACCA TCAATAACCA AGCAGCACTC CCATGCACAG
    GCCACTCTGA ACCCTGTCAC TCCAGCCTCA GAACGCACAG TTGCCTGGGT ATCCAACATG
    CCCCACCTTT CAGCAGATAT TGAGAGCGCT CACATAGAAC GGGAGGAGTA TAAGTTGAAG
    GAATACTCTA AATCAATGGA TGAGAGCCGT CTTGAGAGGG TGAAAGAGTA TGAGGAAGAG
    ATTTACTCCT TAAAAGAGAG GCTGTTGATG TCAAACCGGA AGCTGGAGGA ATATGAGAGG
    CGTCTTCTTA CACAGGAAGA ACAAACTAAT AAGATGATGC AGCAATATCA GTCACGCCTG
    GACCAGAGTG AAAAGCGTTT GCGACAGCAG CAGGTTGAGA AGGACACGCA GATAAAGAGC
    ATCTTAAACA GGTTAGAAAT GGTTGATGCT GGTGGAGGAG GAGATCAGGA AGGATCACTC
    ACAAGAACCA ATCGATATGA AGAGGAGACT TATCGAGGCT CAGAGAAGAA GTTTCCCTCC
    GTGGGTACAG CAGACCCGCG TCTGAGGCTG GCGACTCCAT GGAATGGGGC CCTCCCGCCT
    CTGCCTCCTC GACTCCAAAT CACAGAAAAT GGAGACTTTC TAAACTCAAG TGACCATCAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031748244.1
    CDS21..3443
    Translation

    Target ORF information:

    RefSeq Version XM_031892384.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031892384.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGATTATC ACCTTGCACG TTCCCGGAGG AAAAGCGTCC CAGGGGGAAA ACAGCCCAGT 
    ATGGACGCCC CTCCCACCGC CCCCTTTCGA CCGTCGCAAG GATTCCTGAG CCGTCGCTTG
    AAGAGCTCTA TAAAGAGGAC AAAGAGTCAG CCCAAATTAG ATAGGACCAG CAGTTTCCGG
    CAAATATTGC CAAGGTTTCG AAGTGCTGAT CATGATAGGG CACGGCTCAT GCAGGCCTTT
    AAAGAGTCAC ACTCTCACGA GTCCTTGCTT AGTCCTAGCA GTGCTGCAGA GGCTTTGGAT
    TTGAACTTGG ATGAGGATTC TGTGGTGAAG CCTGTACACA GCAGCATCTT GGGACAGGAG
    TTTTGCTTCG AGGTGACCAC ATCATCAGGT ACCAAATGTT TTGCCTGCCG TTCTGCTGCT
    GAAAGAGACA AATGGATTGA GAATCTTCAA AGAGCTGTAA AGCCCAATAA GGATAACAGC
    AGGCGGGTAG ATAATGTCTT AAAGCTGTGG ATTATTGAAG CCCGTGATCT TCCCCCCAAG
    AAGCGCTATT ATTGTGAGCT CTGTTTGGAT GACATTTTGT ATGCCCGTAC CACCAGCAAG
    CCTCGGAATG ACACTGTCTT TTGGGGGGAA CACTTTGAAT TTAATAACCT TCCAGCTGTT
    CGGAACTTGC GCTTGCACCT CTATAAGGAC ACAGACAAGA AAAGGCGCAA AGAAAAGAGC
    AACTATGTGG GCATGGTGAA CGTGCCTATT GCCAGCATTA GTGGGCGCCA CTTTGTGGAG
    CAGTGGTACC CGGTTTCTCA GCCCACATCC TACAAGGGGA AGAGCAGCTG TCCCTCCATT
    CGCCTAAAAT CCCGATACCA AACCATGAGC ATCCTCCCCA TGGAGCTGTA CAAGGAGTTT
    GCAGAGTATG TCACCAACAA CTACCGGATG CTGTGCGCTG TACTGGAGCC TGTGCTTAGC
    GTGAAGAGCA AAGAGGAGGT TGCCTGTGCT TTAGTGCATA TACTGCAAAG CACAGGCAAG
    GCCAAGGACT TCCTTTCAGA CATGGCTATG ACCGAGGTTG ATCGCTTCCT TGACAGAGAG
    CACCTTATTC TCCGTGAGAA CACACTTGCA ACTAAAGCCA TAGAGGAGTA TCTGAAACTG
    ATAGGACAGA GATACCTCAA GGAAGCCATT GGTGAGTTTA TTCGGGCACT GTATGAGTCT
    GAGGAGGACT GTGAGGTTGA TCCCATGCGT TGTCCACCCT CTGCATTGCC CGATCACCAG
    GCCAATCTCA GGATGTGCTG TGAACTCGCC CTTTGCAAAG TCTTTAACTC TCATTGTGTG
    TTTCCTCGGG AGTTGAAGGA AGTCTTTGCA TCTTGGAGGA TGCGCTGTGC AGAGCGCGGC
    CGGGAAGATA TAGCAGATCG ACTCATCAGT GCCTCTCTCT TCCTCCGATT CCTGTGTCCG
    GCCATTATGT CCCCCAGTCT CTTCAGCCTA ATGCAGGAAT ACCCTGATGA GCAAACATCT
    AGGACCCTCA CGCTCATCGC AAAAGTCATC CAGAACCTGG CCAACTTCAA CTACAACAGG
    TTCAGCAGTA AAGAAGAATA TATGTCCTTT ATGAATGAGT TCCTAGAGAC GGAGTTGGCG
    AATATGCAGA ATTTCCTGTA TGAGATCTCA AACCTGGATT CGCTCACCAA CAGCATTAAC
    TTTGAGGGCT ACATTGACCT TGGGCGGGAG CTGAGTACAC TTCATGCCCT GCTTTGGGAA
    GTGATCCCAC AACTTAGTAA GGATGCACTT ATAAAGTTGG GGCCTCTTCC ACGCCTCCTT
    AATGACATCA GCCTAGCTCT CAGGAACCCC CACATCCAAC GACAACCAAG CCACCAAGAA
    CGACTGCCAC CCCCCAAACC TGTCATTCTG AGGGGACCTT CAGCTGAGCT GCAAAGCTAC
    TTGGTGCGAG ACCTCAACAG CTCTATTGAC ATGACTCGCC TACCCTCACC TACTAAGGAT
    AAACCATCAA AAGATATGTT TTTTGTTTCA CGTCGCCCGC TAACTCGCTC CTCGCCAGCC
    TATTGCACCA GCAGCTCAGA CATCACAGAA CCAGACCAGA AGATGCTGAG CGTGAACAAG
    AGTGTGTCCA TGATGGACTT GCAGGACAGC CGAATGAATA GCGTAACCAA CTTGCAGGCC
    GTAGGTGATC TGCTGAACTC CAGCCAGGCC TCCATATCAG GGGGCCTACG TCCGGGGGGC
    CGGCTCTCCC AGGGCAGTGG ATCCAGCATT GCAGCGGGTC TGAGGCTCAG TCAGATGGGG
    GTCACCACTG ATGGGCTCTC AGCTCAACAG CTACGCATTC CCTTGTCCTT CCAGAACCCG
    CTCTTCCACA TGGCTCCCGA TGGGCCTGGT GGGCCCCCAT CTCATCCTGC TCCTCATCGA
    CCAGACCCCG GAGCTGATGC CTTTGCCCCT TTCCATGGGT ATAGCAAGAG TGAGGACTTG
    AGCTCCGGAA AGCATATGCT TCACAGTCAC AGCTACAGCG ACGAATTCAG CCGGCAGGGG
    TCAGAGTTCA GTAAAAGGCA GTTATCCCTC CAGGACAATC TCCAGCACAT GCTTTCACCC
    CCTCAAATAA CCATTGGCCC TGGAGTGCCA CGAAGGCAAG ACTCACAGCC TCCCCCTTTG
    CAGAGGGGAA AATCCCAACA ACTGACGGTG CAGGCAGCGC ATAAACCTCG GCCAAGCAGT
    GGCAACCTCC TACAGAGCCC AGAACCAATG TATGGGGCAG CAAGAATTAG GCAGCAGTCA
    GTTGAAAAGG ATGCAGGTGG CTTGAAACCA TCAATAACCA AGCAGCACTC CCATGCACAG
    GCCACTCTGA ACCCTGTCAC TCCAGCCTCA GAACGCACAG TTGCCTGGGT ATCCAACATG
    CCCCACCTTT CAGCAGATAT TGAGAGCGCT CACATAGAAC GGGAGGAGTA TAAGTTGAAG
    GAATACTCTA AATCAATGGA TGAGAGCCGT CTTGAGAGGG TGAAAGAGTA TGAGGAAGAG
    ATTTACTCCT TAAAAGAGAG GCTGTTGATG TCAAACCGGA AGCTGGAGGA ATATGAGAGG
    CGTCTTCTTA CACAGGAAGA ACAAACTAAT AAGATGATGC AGCAATATCA GTCACGCCTG
    GACCAGAGTG AAAAGCGTTT GCGACAGCAG CAGGTTGAGA AGGACACGCA GATAAAGAGC
    ATCTTAAACA GGTTAGAAAT GGTTGATGCT GGTGGAGGAG GAGATCAGGA AGGATCACTC
    ACAAGAACCA ATCGATATGA AGAGGAGACT TATCGAGGCT CAGAGAAGAA GTTTCCCTCC
    GTGGGTACAG CAGACCCGCG TCTGAGGCTG GCGACTCCAT GGAATGGGGC CCTCCCGCCT
    CTGCCTCCTC GACTCCAAAT CACAGAAAAT GGAGACTTTC TAAACTCAAG TGACCATCAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa65738
    Clone ID Related Accession (Same CDS sequence) XM_031892385.1
    Accession Version XM_031892385.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3363bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ras/Rap GTPase-activating protein SynGAP isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGACGCCC CTCCCACCGC CCCCTTTCGA CCGTCGCAAG GATTCCTGAG CCGTCGCTTG 
    AAGAGCTCTA TAAAGAGGAC AAAGAGTCAG CCCAAATTAG ATAGGACCAG CAGTTTCCGG
    CAAATATTGC CAAGGTTTCG AAGTGCTGAT CATGATAGGG CACGGCTCAT GCAGGCCTTT
    AAAGAGTCAC ACTCTCACGA GTCCTTGCTT AGTCCTAGCA GTGCTGCAGA GGCTTTGGAT
    TTGAACTTGG ATGAGGATTC TGTGGTGAAG CCTGTACACA GCAGCATCTT GGGACAGGAG
    TTTTGCTTCG AGGTGACCAC ATCATCAGGT ACCAAATGTT TTGCCTGCCG TTCTGCTGCT
    GAAAGAGACA AATGGATTGA GAATCTTCAA AGAGCTGTAA AGCCCAATAA GGATAACAGC
    AGGCGGGTAG ATAATGTCTT AAAGCTGTGG ATTATTGAAG CCCGTGATCT TCCCCCCAAG
    AAGCGCTATT ATTGTGAGCT CTGTTTGGAT GACATTTTGT ATGCCCGTAC CACCAGCAAG
    CCTCGGAATG ACACTGTCTT TTGGGGGGAA CACTTTGAAT TTAATAACCT TCCAGCTGTT
    CGGAACTTGC GCTTGCACCT CTATAAGGAC ACAGACAAGA AAAGGCGCAA AGAAAAGAGC
    AACTATGTGG GCATGGTGAA CGTGCCTATT GCCAGCATTA GTGGGCGCCA CTTTGTGGAG
    CAGTGGTACC CGGTTTCTCA GCCCACATCC TACAAGGGGA AGAGCAGCTG TCCCTCCATT
    CGCCTAAAAT CCCGATACCA AACCATGAGC ATCCTCCCCA TGGAGCTGTA CAAGGAGTTT
    GCAGAGTATG TCACCAACAA CTACCGGATG CTGTGCGCTG TACTGGAGCC TGTGCTTAGC
    GTGAAGAGCA AAGAGGAGGT TGCCTGTGCT TTAGTGCATA TACTGCAAAG CACAGGCAAG
    GCCAAGGACT TCCTTTCAGA CATGGCTATG ACCGAGGTTG ATCGCTTCCT TGACAGAGAG
    CACCTTATTC TCCGTGAGAA CACACTTGCA ACTAAAGCCA TAGAGGAGTA TCTGAAACTG
    ATAGGACAGA GATACCTCAA GGAAGCCATT GGTGAGTTTA TTCGGGCACT GTATGAGTCT
    GAGGAGGACT GTGAGGTTGA TCCCATGCGT TGTCCACCCT CTGCATTGCC CGATCACCAG
    GCCAATCTCA GGATGTGCTG TGAACTCGCC CTTTGCAAAG TCTTTAACTC TCATTGTGTG
    TTTCCTCGGG AGTTGAAGGA AGTCTTTGCA TCTTGGAGGA TGCGCTGTGC AGAGCGCGGC
    CGGGAAGATA TAGCAGATCG ACTCATCAGT GCCTCTCTCT TCCTCCGATT CCTGTGTCCG
    GCCATTATGT CCCCCAGTCT CTTCAGCCTA ATGCAGGAAT ACCCTGATGA GCAAACATCT
    AGGACCCTCA CGCTCATCGC AAAAGTCATC CAGAACCTGG CCAACTTCAA CTACAACAGG
    TTCAGCAGTA AAGAAGAATA TATGTCCTTT ATGAATGAGT TCCTAGAGAC GGAGTTGGCG
    AATATGCAGA ATTTCCTGTA TGAGATCTCA AACCTGGATT CGCTCACCAA CAGCATTAAC
    TTTGAGGGCT ACATTGACCT TGGGCGGGAG CTGAGTACAC TTCATGCCCT GCTTTGGGAA
    GTGATCCCAC AACTTAGTAA GGATGCACTT ATAAAGTTGG GGCCTCTTCC ACGCCTCCTT
    AATGACATCA GCCTAGCTCT CAGGAACCCC CACATCCAAC GACAACCAAG CCACCAAGAA
    CGACTGCCAC CCCCCAAACC TGTCATTCTG AGGGGACCTT CAGCTGAGCT GCAAAGCTAC
    TTGGTGCGAG ACCTCAACAG CTCTATTGAC ATGACTCGCC TACCCTCACC TACTAAGGAT
    AAACCATCAA AAGATATGTT TTTTGTTTCA CGTCGCCCGC TAACTCGCTC CTCGCCAGCC
    TATTGCACCA GCAGCTCAGA CATCACAGAA CCAGACCAGA AGATGCTGAG CGTGAACAAG
    AGTGTGTCCA TGATGGACTT GCAGGACAGC CGAATGAATA GCGTAACCAA CTTGCAGGCC
    GTAGGTGATC TGCTGAACTC CAGCCAGGCC TCCATATCAG GGGGCCTACG TCCGGGGGGC
    CGGCTCTCCC AGGGCAGTGG ATCCAGCATT GCAGCGGGTC TGAGGCTCAG TCAGATGGGG
    GTCACCACTG ATGGGCTCTC AGCTCAACAG CTACGCATTC CCTTGTCCTT CCAGAACCCG
    CTCTTCCACA TGGCTCCCGA TGGGCCTGGT GGGCCCCCAT CTCATCCTGC TCCTCATCGA
    CCAGACCCCG GAGCTGATGC CTTTGCCCCT TTCCATGGGT ATAGCAAGAG TGAGGACTTG
    AGCTCCGGAA AGCATATGCT TCACAGTCAC AGCTACAGCG ACGAATTCAG CCGGCAGGGG
    TCAGAGTTCA GTAAAAGGCA GTTATCCCTC CAGGACAATC TCCAGCACAT GCTTTCACCC
    CCTCAAATAA CCATTGGCCC TGGAGTGCCA CGAAGGCAAG ACTCACAGCC TCCCCCTTTG
    CAGAGGGGAA AATCCCAACA ACTGACGGTG CAGGCAGCGC ATAAACCTCG GCCAAGCAGT
    GGCAACCTCC TACAGAGCCC AGAACCAATG TATGGGGCAG CAAGAATTAG GCAGCAGTCA
    GTTGAAAAGG ATGCAGGTGG CTTGAAACCA TCAATAACCA AGCAGCACTC CCATGCACAG
    GCCACTCTGA ACCCTGTCAC TCCAGCCTCA GAACGCACAG TTGCCTGGGT ATCCAACATG
    CCCCACCTTT CAGCAGATAT TGAGAGCGCT CACATAGAAC GGGAGGAGTA TAAGTTGAAG
    GAATACTCTA AATCAATGGA TGAGAGCCGT CTTGAGAGGG TGAAAGAGTA TGAGGAAGAG
    ATTTACTCCT TAAAAGAGAG GCTGTTGATG TCAAACCGGA AGCTGGAGGA ATATGAGAGG
    CGTCTTCTTA CACAGGAAGA ACAAACTAAT AAGATGATGC AGCAATATCA GTCACGCCTG
    GACCAGAGTG AAAAGCGTTT GCGACAGCAG CAGGTTGAGA AGGACACGCA GATAAAGAGC
    ATCTTAAACA GGTTAGAAAT GGTTGATGCT GGTGGAGGAG GAGATCAGGA AGGATCACTC
    ACAAGAACCA ATCGATATGA AGAGGAGACT TATCGAGGCT CAGAGAAGAA GTTTCCCTCC
    GTGGGTACAG CAGACCCGCG TCTGAGGCTG GCGACTCCAT GGAATGGGGC CCTCCCGCCT
    CTGCCTCCTC GACTCCAAAT CACAGAAAAT GGAGACTTTC TAAACTCAAG TGACCATCAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031748245.1
    CDS507..3869
    Translation

    Target ORF information:

    RefSeq Version XM_031892385.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis synaptic Ras GTPase activating protein 1 (syngap1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031892385.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGACGCCC CTCCCACCGC CCCCTTTCGA CCGTCGCAAG GATTCCTGAG CCGTCGCTTG 
    AAGAGCTCTA TAAAGAGGAC AAAGAGTCAG CCCAAATTAG ATAGGACCAG CAGTTTCCGG
    CAAATATTGC CAAGGTTTCG AAGTGCTGAT CATGATAGGG CACGGCTCAT GCAGGCCTTT
    AAAGAGTCAC ACTCTCACGA GTCCTTGCTT AGTCCTAGCA GTGCTGCAGA GGCTTTGGAT
    TTGAACTTGG ATGAGGATTC TGTGGTGAAG CCTGTACACA GCAGCATCTT GGGACAGGAG
    TTTTGCTTCG AGGTGACCAC ATCATCAGGT ACCAAATGTT TTGCCTGCCG TTCTGCTGCT
    GAAAGAGACA AATGGATTGA GAATCTTCAA AGAGCTGTAA AGCCCAATAA GGATAACAGC
    AGGCGGGTAG ATAATGTCTT AAAGCTGTGG ATTATTGAAG CCCGTGATCT TCCCCCCAAG
    AAGCGCTATT ATTGTGAGCT CTGTTTGGAT GACATTTTGT ATGCCCGTAC CACCAGCAAG
    CCTCGGAATG ACACTGTCTT TTGGGGGGAA CACTTTGAAT TTAATAACCT TCCAGCTGTT
    CGGAACTTGC GCTTGCACCT CTATAAGGAC ACAGACAAGA AAAGGCGCAA AGAAAAGAGC
    AACTATGTGG GCATGGTGAA CGTGCCTATT GCCAGCATTA GTGGGCGCCA CTTTGTGGAG
    CAGTGGTACC CGGTTTCTCA GCCCACATCC TACAAGGGGA AGAGCAGCTG TCCCTCCATT
    CGCCTAAAAT CCCGATACCA AACCATGAGC ATCCTCCCCA TGGAGCTGTA CAAGGAGTTT
    GCAGAGTATG TCACCAACAA CTACCGGATG CTGTGCGCTG TACTGGAGCC TGTGCTTAGC
    GTGAAGAGCA AAGAGGAGGT TGCCTGTGCT TTAGTGCATA TACTGCAAAG CACAGGCAAG
    GCCAAGGACT TCCTTTCAGA CATGGCTATG ACCGAGGTTG ATCGCTTCCT TGACAGAGAG
    CACCTTATTC TCCGTGAGAA CACACTTGCA ACTAAAGCCA TAGAGGAGTA TCTGAAACTG
    ATAGGACAGA GATACCTCAA GGAAGCCATT GGTGAGTTTA TTCGGGCACT GTATGAGTCT
    GAGGAGGACT GTGAGGTTGA TCCCATGCGT TGTCCACCCT CTGCATTGCC CGATCACCAG
    GCCAATCTCA GGATGTGCTG TGAACTCGCC CTTTGCAAAG TCTTTAACTC TCATTGTGTG
    TTTCCTCGGG AGTTGAAGGA AGTCTTTGCA TCTTGGAGGA TGCGCTGTGC AGAGCGCGGC
    CGGGAAGATA TAGCAGATCG ACTCATCAGT GCCTCTCTCT TCCTCCGATT CCTGTGTCCG
    GCCATTATGT CCCCCAGTCT CTTCAGCCTA ATGCAGGAAT ACCCTGATGA GCAAACATCT
    AGGACCCTCA CGCTCATCGC AAAAGTCATC CAGAACCTGG CCAACTTCAA CTACAACAGG
    TTCAGCAGTA AAGAAGAATA TATGTCCTTT ATGAATGAGT TCCTAGAGAC GGAGTTGGCG
    AATATGCAGA ATTTCCTGTA TGAGATCTCA AACCTGGATT CGCTCACCAA CAGCATTAAC
    TTTGAGGGCT ACATTGACCT TGGGCGGGAG CTGAGTACAC TTCATGCCCT GCTTTGGGAA
    GTGATCCCAC AACTTAGTAA GGATGCACTT ATAAAGTTGG GGCCTCTTCC ACGCCTCCTT
    AATGACATCA GCCTAGCTCT CAGGAACCCC CACATCCAAC GACAACCAAG CCACCAAGAA
    CGACTGCCAC CCCCCAAACC TGTCATTCTG AGGGGACCTT CAGCTGAGCT GCAAAGCTAC
    TTGGTGCGAG ACCTCAACAG CTCTATTGAC ATGACTCGCC TACCCTCACC TACTAAGGAT
    AAACCATCAA AAGATATGTT TTTTGTTTCA CGTCGCCCGC TAACTCGCTC CTCGCCAGCC
    TATTGCACCA GCAGCTCAGA CATCACAGAA CCAGACCAGA AGATGCTGAG CGTGAACAAG
    AGTGTGTCCA TGATGGACTT GCAGGACAGC CGAATGAATA GCGTAACCAA CTTGCAGGCC
    GTAGGTGATC TGCTGAACTC CAGCCAGGCC TCCATATCAG GGGGCCTACG TCCGGGGGGC
    CGGCTCTCCC AGGGCAGTGG ATCCAGCATT GCAGCGGGTC TGAGGCTCAG TCAGATGGGG
    GTCACCACTG ATGGGCTCTC AGCTCAACAG CTACGCATTC CCTTGTCCTT CCAGAACCCG
    CTCTTCCACA TGGCTCCCGA TGGGCCTGGT GGGCCCCCAT CTCATCCTGC TCCTCATCGA
    CCAGACCCCG GAGCTGATGC CTTTGCCCCT TTCCATGGGT ATAGCAAGAG TGAGGACTTG
    AGCTCCGGAA AGCATATGCT TCACAGTCAC AGCTACAGCG ACGAATTCAG CCGGCAGGGG
    TCAGAGTTCA GTAAAAGGCA GTTATCCCTC CAGGACAATC TCCAGCACAT GCTTTCACCC
    CCTCAAATAA CCATTGGCCC TGGAGTGCCA CGAAGGCAAG ACTCACAGCC TCCCCCTTTG
    CAGAGGGGAA AATCCCAACA ACTGACGGTG CAGGCAGCGC ATAAACCTCG GCCAAGCAGT
    GGCAACCTCC TACAGAGCCC AGAACCAATG TATGGGGCAG CAAGAATTAG GCAGCAGTCA
    GTTGAAAAGG ATGCAGGTGG CTTGAAACCA TCAATAACCA AGCAGCACTC CCATGCACAG
    GCCACTCTGA ACCCTGTCAC TCCAGCCTCA GAACGCACAG TTGCCTGGGT ATCCAACATG
    CCCCACCTTT CAGCAGATAT TGAGAGCGCT CACATAGAAC GGGAGGAGTA TAAGTTGAAG
    GAATACTCTA AATCAATGGA TGAGAGCCGT CTTGAGAGGG TGAAAGAGTA TGAGGAAGAG
    ATTTACTCCT TAAAAGAGAG GCTGTTGATG TCAAACCGGA AGCTGGAGGA ATATGAGAGG
    CGTCTTCTTA CACAGGAAGA ACAAACTAAT AAGATGATGC AGCAATATCA GTCACGCCTG
    GACCAGAGTG AAAAGCGTTT GCGACAGCAG CAGGTTGAGA AGGACACGCA GATAAAGAGC
    ATCTTAAACA GGTTAGAAAT GGTTGATGCT GGTGGAGGAG GAGATCAGGA AGGATCACTC
    ACAAGAACCA ATCGATATGA AGAGGAGACT TATCGAGGCT CAGAGAAGAA GTTTCCCTCC
    GTGGGTACAG CAGACCCGCG TCTGAGGCTG GCGACTCCAT GGAATGGGGC CCTCCCGCCT
    CTGCCTCCTC GACTCCAAAT CACAGAAAAT GGAGACTTTC TAAACTCAAG TGACCATCAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.