dio2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	dio2
Entrez Gene ID	100036737
Full Name	deiodinase, iodothyronine, type 2
Synonyms	5dii,DI-2,sely,txdi2
General protein information	Preferred Names deiodinase, iodothyronine, type 2 Names type II iodothyronine deiodinase deiodinase II iodothyronine type II deiodinase type 2 deiodinase
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)
Summary	The protein encoded by this gene belongs to the iodothyronine deiodinase family. It catalyzes the conversion of prohormone thyroxine (3,5,3',5'-tetraiodothyronine, T4) to the bioactive thyroid hormone (3,5,3'-triiodothyronine, T3) by outer ring 5'-deiodination. This enzyme is thought to be responsible for the 'local' production of T3, and thus important in influencing thyroid hormone action in those peripheral tissues where it is expressed. This gene is also essential for early development in amphibians, and its expression has been observed in early-tailbud-stage embryos before the development of a functional thyroid gland. This protein is a selenoprotein containing the rare selenocysteine (Sec) amino acid at its active site, and may contain additional Sec residues. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. [provided by RefSeq, May 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001197232.4	NP_001184161.3	type II iodothyronine deiodinase
NM_001197232.3	NP_001184161.2	type II iodothyronine deiodinase

Pathways from BioSystems

Homologies

Homo sapiens (human)	DIO2	NP_001007024.1
Bos taurus (cattle)	DIO2	NP_001010992.1
Danio rerio (zebrafish)	dio2	NP_997954.2
Xenopus tropicalis (tropical clawed frog)	dio2	NP_001184161.2
Pan troglodytes (chimpanzee)	DIO2	NP_001116109.1
Canis lupus familiaris (dog)	DIO2	NP_001116117.1
	DIO2	NP_001152766.1
Mus musculus (house mouse)	Dio2	NP_034180.1
Rattus norvegicus (Norway rat)	Dio2	NP_113908.3
Gallus gallus (chicken)	DIO2	NP_989445.2

Gene Ontology
Bibliography

Related articles in PubMed

Composition and evolution of the vertebrate and mammalian selenoproteomes.
Mariotti M, Ridge PG, Zhang Y, Lobanov AV, Pringle TH, Guigo R, Hatfield DL, Gladyshev VN
PloS one7(3)e33066(2012)

Expression of enzymes involved in thyroid hormone metabolism during the early development of Xenopus tropicalis.
Tindall AJ, Morris ID, Pownall ME, Isaacs HV
Biology of the cell99(3)151-63(2007 Mar)

Mutation of the Secys residue 266 in human type 2 selenodeiodinase alters 75Se incorporation without affecting its biochemical properties.
Salvatore D, Harney JW, Larsen PR
Biochimie81(5)535-8(1999 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following dio2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dio2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa03089	NM_001197232.3 Latest version!	Xenopus tropicalis deiodinase, iodothyronine, type 2 (dio2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OXa47225	NM_001197232.4 Latest version!	Xenopus tropicalis deiodinase, iodothyronine, type 2 (dio2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dio2 ( NM_001197232.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001184161.2 Xenopus tropicalis deiodinase, iodothyronine, type 2 (dio2), mRNA.

Price & Availability↑

CloneID	OXa03089
Clone ID Related Accession (Same CDS sequence)	NM_001197232.3
Accession Version	NM_001197232.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-11
Organism	Xenopus tropicalis(tropical clawed frog)
Product	type II iodothyronine deiodinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AAMC03031889.1, CX813198.1 and CX974560.1. On May 12, 2016 this sequence version replaced NM_001197232.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: CX813198.1, EF052283.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1025683, SAMEA1696413 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: PMID: 17073826 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGACTGC TGAGTGTGGA CTTGTTGATC ACCCTGCAGA TCTTGCCAGG TTTCTTCTCA 
AACTGCCTGT TCCTGGCTCT GTACGACTCT GTCGTGCTAG TAAAACACGT GCTTCTACAA 
CTCAGCCGCT CCAAATCCAG CCAGAGTCAA TGGCGCCGAA TGCTCACCCC AGAAGGGCTG 
CGCTGTGTGT GGAACAGCTT CCTATTGGAT GCCTACAAGC AGGTTAAGCT GGGACAAGAT 
GCTCCAAATT CCAATGTTAT TCAAGTGTCA AACAAAATTT GTAAAAGTGT ACAAAGAAAA 
CTTGTAGGAA AATGCCACCT TTTAGACTTT GCAAGCTCTG AGCGCCCACT TGTTGTCAAT 
TTTGGATCAG CTACCTGACC TCCATTCATA AGCCAGCTGC CAGCCTTCAG CAAACTGGTG 
GAAGAGTTCT CTAGTGTTGC CGACTTTGTG TTGGTGTACA TTGACGAGGC ACATCCCTCA 
GATGGCTGGG CTGCCCCTGG TACAACATCC TATGAAGTAA AGAAGCACAG AAACCAAGAA 
GAACGTTGTG CAGCTGCCAG CAAGCTTTTA GAACATTTCT CTATCCCTCC ACAATGCCAG 
GTCGTTGCTG ACTGCATGGA CAATAATGCC AATGTTGCAT ATGGTGTCTC TTTTGAGAGA 
GTATGTATTG TACAGAAGCA AAAGATTGTA TATCTGGGAG GTAAAGGACC TTTCTTTTAT 
AACATCCAGG AAATCCGCCG GTGGCTGGAA CTTAGCTTTG GGAAGAGATG AACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001184161.2
CDS	544..1320
Translation	MGLLSVDLLITLQILPGFFSNCLFLALYDSVVLVKHVLLQLSRSKSSQSQWRRMLTPEGLRCVWNSFLLDAYKQVKLGQDAPNSNVIQVSNKICKSVQRKLVGKCHLLDFASSERPLVVNFGSATPPFISQLPAFSKLVEEFSSVADFVLVYIDEAHPSDGWAAPGTTSYEVKKHRNQEERCAAASKLLEHFSIPPQCQVVADCMDNNANVAYGVSFERVCIVQKQKIVYLGGKGPFFYNIQEIRRWLELSFGKRT

Target ORF information:

RefSeq Version	NM_001197232.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis deiodinase, iodothyronine, type 2 (dio2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001197232.3

ORF Insert Sequence:

ATGGGACTGC TGAGTGTGGA CTTGTTGATC ACCCTGCAGA TCTTGCCAGG TTTCTTCTCA 
AACTGCCTGT TCCTGGCTCT GTACGACTCT GTCGTGCTAG TAAAACACGT GCTTCTACAA 
CTCAGCCGCT CCAAATCCAG CCAGAGTCAA TGGCGCCGAA TGCTCACCCC AGAAGGGCTG 
CGCTGTGTGT GGAACAGCTT CCTATTGGAT GCCTACAAGC AGGTTAAGCT GGGACAAGAT 
GCTCCAAATT CCAATGTTAT TCAAGTGTCA AACAAAATTT GTAAAAGTGT ACAAAGAAAA 
CTTGTAGGAA AATGCCACCT TTTAGACTTT GCAAGCTCTG AGCGCCCACT TGTTGTCAAT 
TTTGGATCAG CTACCTGACC TCCATTCATA AGCCAGCTGC CAGCCTTCAG CAAACTGGTG 
GAAGAGTTCT CTAGTGTTGC CGACTTTGTG TTGGTGTACA TTGACGAGGC ACATCCCTCA 
GATGGCTGGG CTGCCCCTGG TACAACATCC TATGAAGTAA AGAAGCACAG AAACCAAGAA 
GAACGTTGTG CAGCTGCCAG CAAGCTTTTA GAACATTTCT CTATCCCTCC ACAATGCCAG 
GTCGTTGCTG ACTGCATGGA CAATAATGCC AATGTTGCAT ATGGTGTCTC TTTTGAGAGA 
GTATGTATTG TACAGAAGCA AAAGATTGTA TATCTGGGAG GTAAAGGACC TTTCTTTTAT 
AACATCCAGG AAATCCGCCG GTGGCTGGAA CTTAGCTTTG GGAAGAGATG AACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dio2 ( NM_001197232.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001184161.3 Xenopus tropicalis deiodinase, iodothyronine, type 2 (dio2), mRNA.

Price & Availability↑

CloneID	OXa47225
Clone ID Related Accession (Same CDS sequence)	NM_001197232.4
Accession Version	NM_001197232.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	type II iodothyronine deiodinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AAMC03031889.1. On Oct 11, 2018 this sequence version replaced NM_001197232.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: CX813198.1, EF052283.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1025683, SAMEA1696413 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: PMID: 17073826 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGACTGC TGAGTGTGGA CTTGTTGATC ACCCTGCAGA TCTTGCCAGG TTTCTTCTCA 
AACTGCCTGT TCCTGGCTCT GTACGACTCT GTCGTGCTAG TAAAACACGT GCTTCTACAA 
CTCAGCCGCT CCAAATCCAG CCAGAGTCAA TGGCGCCGAA TGCTCACCCC AGAAGGGCTG 
CGCTGTGTGT GGAACAGCTT CCTATTGGAT GCCTACAAGC AGGTTAAGCT GGGACAAGAT 
GCTCCAAATT CCAATGTTAT TCAAGTGTCA AACAAAATTT GTAAAAGTGT ACAAAGAAAA 
CTTGTAGGAA AATGCCACCT TTTAGACTTT GCAAGCTCTG AGCGCCCACT TGTTGTCAAT 
TTTGGATCAG CTACCTGACC TCCATTCATA AGCCAGCTGC CAGCCTTCAG CAAACTGGTG 
GAAGAGTTCT CTAGTGTTGC CGACTTTGTG TTGGTGTACA TTGACGAGGC ACATCCCTCA 
GATGGCTGGG CTGCCCCTGG TACAACATCC TATGAAGTAA AGAAGCACAG AAACCAAGAA 
GAACGTTGTG CAGCTGCCAG CAAGCTTTTA GAACATTTCT CTATCCCTCC ACAATGCCAG 
GTCGTTGCTG ACTGCATGGA CAATAATGCC AATGTTGCAT ATGGTGTCTC TTTTGAGAGA 
GTATGTATTG TACAGAAGCA AAAGATTGTA TATCTGGGAG GTAAAGGACC TTTCTTTTAT 
AACATCCAGG AAATCCGCCG GTGGCTGGAA CTTAGCTTTG GGAAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001184161.3
CDS	544..1314
Translation	MGLLSVDLLITLQILPGFFSNCLFLALYDSVVLVKHVLLQLSRSKSSQSQWRRMLTPEGLRCVWNSFLLDAYKQVKLGQDAPNSNVIQVSNKICKSVQRKLVGKCHLLDFASSERPLVVNFGSAT*PPFISQLPAFSKLVEEFSSVADFVLVYIDEAHPSDGWAAPGTTSYEVKKHRNQEERCAAASKLLEHFSIPPQCQVVADCMDNNANVAYGVSFERVCIVQKQKIVYLGGKGPFFYNIQEIRRWLELSFGKR

Target ORF information:

RefSeq Version	NM_001197232.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis deiodinase, iodothyronine, type 2 (dio2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001197232.4

ORF Insert Sequence:

ATGGGACTGC TGAGTGTGGA CTTGTTGATC ACCCTGCAGA TCTTGCCAGG TTTCTTCTCA 
AACTGCCTGT TCCTGGCTCT GTACGACTCT GTCGTGCTAG TAAAACACGT GCTTCTACAA 
CTCAGCCGCT CCAAATCCAG CCAGAGTCAA TGGCGCCGAA TGCTCACCCC AGAAGGGCTG 
CGCTGTGTGT GGAACAGCTT CCTATTGGAT GCCTACAAGC AGGTTAAGCT GGGACAAGAT 
GCTCCAAATT CCAATGTTAT TCAAGTGTCA AACAAAATTT GTAAAAGTGT ACAAAGAAAA 
CTTGTAGGAA AATGCCACCT TTTAGACTTT GCAAGCTCTG AGCGCCCACT TGTTGTCAAT 
TTTGGATCAG CTACCTGACC TCCATTCATA AGCCAGCTGC CAGCCTTCAG CAAACTGGTG 
GAAGAGTTCT CTAGTGTTGC CGACTTTGTG TTGGTGTACA TTGACGAGGC ACATCCCTCA 
GATGGCTGGG CTGCCCCTGG TACAACATCC TATGAAGTAA AGAAGCACAG AAACCAAGAA 
GAACGTTGTG CAGCTGCCAG CAAGCTTTTA GAACATTTCT CTATCCCTCC ACAATGCCAG 
GTCGTTGCTG ACTGCATGGA CAATAATGCC AATGTTGCAT ATGGTGTCTC TTTTGAGAGA 
GTATGTATTG TACAGAAGCA AAAGATTGTA TATCTGGGAG GTAAAGGACC TTTCTTTTAT 
AACATCCAGG AAATCCGCCG GTGGCTGGAA CTTAGCTTTG GGAAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Composition and evolution of the vertebrate and mammalian selenoproteomes.
PloS one7(3)e33066(2012)
Mariotti M,Ridge PG,Zhang Y,Lobanov AV,Pringle TH,Guigo R,Hatfield DL,Gladyshev VN

Expression of enzymes involved in thyroid hormone metabolism during the early development of Xenopus tropicalis.
Biology of the cell99(3)151-63(2007 Mar)
Tindall AJ,Morris ID,Pownall ME,Isaacs HV

Mutation of the Secys residue 266 in human type 2 selenodeiodinase alters 75Se incorporation without affecting its biochemical properties.
Biochimie81(5)535-8(1999 May)
Salvatore D,Harney JW,Larsen PR

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dio2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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