nid2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	nid2
Entrez Gene ID	100036716
Full Name	nidogen 2
Synonyms	nid-2,nid1,nidogen-2
General protein information	Preferred Names nidogen 2 Names nidogen-2 nidogen 1 nidogen 2 (osteonidogen) osteonidogen
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012967809.2	XP_012823263.1	nidogen-2 isoform X1
XM_012967811.2	XP_012823265.1	nidogen-2 isoform X4
NM_001097263.1	NP_001090732.1	nidogen-2 precursor
XM_018096080.1	XP_017951569.1	nidogen-2 isoform X2
XM_012967811.3	XP_012823265.2	nidogen-2 isoform X2
XM_012967810.2	XP_012823264.1	nidogen-2 isoform X3
XM_012967809.3	XP_012823263.2	nidogen-2 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	NID2	NP_031387.3
Canis lupus familiaris (dog)	NID2	XP_537445.4
Mus musculus (house mouse)	Nid2	NP_032721.2
Danio rerio (zebrafish)	nid2a	XP_005169909.1
Gallus gallus (chicken)	NID2	XP_421471.4
	NID2	XP_001098764.1
Xenopus tropicalis (tropical clawed frog)	nid2	NP_001090732.1
Pan troglodytes (chimpanzee)	NID2	XP_001158556.2
Rattus norvegicus (Norway rat)	Nid2	NP_001012005.2

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following nid2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nid2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa63347	XM_012967811.3 Latest version!	Xenopus tropicalis nidogen 2 (nid2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa63346	XM_012967809.3 Latest version!	Xenopus tropicalis nidogen 2 (nid2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa23690	NM_001097263.1 Latest version!	Xenopus tropicalis nidogen 2 (nid2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa29902	XM_012967809.2 Latest version!	Xenopus tropicalis nidogen 2 (nid2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa29903	XM_012967810.2 Latest version!	Xenopus tropicalis nidogen 2 (nid2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OXa29904	XM_012967811.2 Latest version!	Xenopus tropicalis nidogen 2 (nid2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OXa45925	XM_018096080.1 Latest version!	Xenopus tropicalis nidogen 2 (nid2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nid2 ( XM_012967811.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823265.2 Xenopus tropicalis nidogen 2 (nid2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa63347
Clone ID Related Accession (Same CDS sequence)	XM_012967811.3
Accession Version	XM_012967811.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4320bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nidogen-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012967811.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGACA GAACTCTGCA TCTGCTCCTG CCCTTGCACT GCCTGCTGGC CTTCTCTGCT 
GCTATATCCC GGCATGAACT CTTCCCCTAT GGGGAACTGA GGGGAGATGT GCTCCTGCAG 
GAGGGGGACG ATGAGACTTC TGAGGTGGTG AAACTCTCCC AACCGCTGCT CTTCTATGAA 
GCCCAATTCA GCAATTTATA TGTAGGGACC AATGGAATTA TTTCCACTCA GGACTTCCCC 
AGAGAAACTC AGTATGTGGA TGATGGCTTC CCTACAGACT TCCCTGTCAT TGCTCCATTC 
CTCTCAGATA TAGATACCAG CAATGGCAAA GGAAAGATTT TTTATCGGCA GGACAATTCT 
GAACAAGTTC TCAATCGGGC AGTCAGTGAG ATCCAGAGGG GTTTCCCAGA GACTTCATTT 
GTTCCTACTG ATGCCTTCCT TGTGACTTGG GAAAATGTTG GTGCCTACGA AGAGATGACC 
CGTCACTCTC CATCGTCTGA TAGGAGAAAC ACGTTCCAGG CGGTACTAGC ATATGATCAC 
TCTGATGCTT ACGCTATATT CCTTTATCCT GAAGATGGAC TCCAGTTTTT TGGTACTCGT 
CCTAAGGAAT CCTACAATGT GCACATTGAG CTGCCTGCCA GAGTGGGCTT TAGCCGATCA 
GAATCGGGAC CATACTACAG TGTAACAAGT AATGAGCAAT CCGTGAAGAA TTTGTATCAA 
ACTGGAAACA CTGGTGTCCC AGGGATTTGG GTCTTTCACA TTGGCAGCAT TTGGGAATTA 
GACAATGTAA TTCCAGCAAA ATTCTTTGGT GCTCCCACAA AGGAACAATC GCTTGAAAAT 
TCACATACAG GTTTCCCAGA ACACAGAATA TTTGAAAAGA ATGAAAGTGA AGACAGTATT 
GATTACCCAG AGTCCTTTTA TGAGGAGAAT GAAGATGATC TGGAAGATAA TACAAGTGAT 
GCCACACCAA CACATAATGC ACACCAAAAA GTTGATTCAG ACACTGGAAA TCAGGGATAC 
TTGGACATAG ATCAGGCTTC CAAACTGACC CATGCAGATG TTGAACAGGA AGCTACATCA 
TCTGTTGTGC TTTCCTCTTC ACATCAGAAT CCTGATTGGA CACCCTACAA ACTCAGTGAA 
TCTGATACTG ACAGAGATGG GCCATTGCAG GAACCTCAAG TTCAGACAGG GGAGGATACA 
GATTTGACAA GGGGAGACAC ATTACTGACT GAAGAGCTGC CATCCCATAA AGCACATAAT 
TTAACACCTC AAAATTCTGA AGATCTCTCA ATATATCCTG AAAGTGAAGT TCTCGTGGGC 
AGTCACCCAG AACAGGAAAA TGATTTTGAA AGAAAGCAGA TTGATGCTGA TCCTGGACTC 
CAAACTGATG TGTTTTCCTT TAACCCTGTG GGCAAGGAGA CATGTGAGAG GAACCACGGG 
AGATGTTCTA GGTTTGCTTT TTGTGCCGAT TACTCTGCTG GCTTCTGCTG TCATTGCCAG 
GAAGATTATT ATGGCAACGG AGTACATTGT TTGCCAAGAG GGGCTCCACA CCGTGTGAAT 
GGTAAAGTGA GCGGAAACAT CCTGGTCGGT CAAACACCTG TCAACTTCAT TGCTGCCGAT 
TTACACGCAT ACATTGTGGC AAATGATGGA AGAGCGTACA CAGCAATCAG CCACGTTCCT 
GATCCTGCAT CCTGGTCTTT AACACCTCTT GCTCCAATTG GGGGACTCTT TGGCTGGCTT 
TTTGCTTTGG AAAAACCAGG CTTTCAAAAT GGTTTTAGCT TTACCGGTGC CAAGTTTGTA 
CACAACATTG ATGTCACATT TTACCCGGGA GAAGAAACTG TTCATATTAC TCAAACAGCA 
GATGGCTTTG ATTCTGAGGG TTATATGAAT GTAAAGACAG CAATTCAGGG CAAAATTCCA 
TATATCCCAG AGACATCGAC CATCAAGCTG TCTCCTTATA CTGAACTGTA TCAATACTCT 
GGCTCAGTTG TAACTTCGAC AGCCTACAGG GAATACACAG TCATCTCAGA ATCAAACGAA 
GAACAAAAAC TGTCTTACCG GTTACGGCAA AATGTCACTT ACCAAGAGTG CAGCCATTCC 
CAGAAACAGC CTGTTAGTGC TCAAAAGCTT TATGTTGATC GTGTCTTTGC CCTTTACAAT 
AAAGAAGAGA AGGTCCTTCG TTATGCAGTT ACAAACTACA TTGGATCTCT GCATGATTCG 
ACTGGACAGG ACGATCTCAC AGTTAACCCG TGCTATGATG GTACCCACTT GTGTGACACA 
AGAGCTAAAT GCCAGCCTGG TAGTGGTCTA GAATACAGTT GTGTTTGTGC GTCTGGATAC 
CAAGGGGATG GAAGAGATTG CACTGATGTA AATGAATGTG AAGTTGGCTT CACCCGGTGC 
GGCCAGAACA CTGTTTGTGT CAACCTCCAG GGGAGTTACC GTTGTGAGTG CGCCAGTGGT 
TTCACTTTAT CTGGAGACGG GCACAACTGC ATCTTGGCTT CACTAATAAA CCCATGTGAA 
GATGGAAGTC ATACATGTAA CAGAGACACA TCACGCTGTG TTCCGCGTGG AGATGGTGTA 
TTCACATGTG AATGTTTCCC TGGATTTAAC AAGAGTGGAG AAGACTGTGT TGATGTTGAT 
GAATGCACTG AGCACAGATG TCATCCAGAT GCCTCATGTA CCAACACCCT GGGCTCTTTC 
TCCTGTCGGT GTAACAGTGG TTATGAAGGA GATGGCTTCC AGTGCACTCA AATCTTAGGT 
CCTGAACCCC TGAAAACCCC CTGTTTAGAA AAGCGCCAGC AGCTACACCC AAGGGGCCCC 
CGTCCAGCTG TGGGACAGTT TGTACCAGAG TGTGATGTAG AGGGAAATTA TGTTCCATTA 
CAGTGTCATG GCAGCACTGG CCATTGTTGG TGTGTGGACA AATTAGGTGA AGAGATTACT 
GGAACAAGAA CACCACCGGG CAGACCAGCC CCAAATTGTG GGGAAAGAGG TCCTGAACCC 
CTGAAAACCC CCTGTTTAGA AAAGCGCCAG CAGCTACACC CAAGGGGCCC CCGTCCAGCT 
GTGGGACAGT TTGTACCAGA GTGTGATGTA GAGGGAAATT ATGTTCCATT ACAGTGTCAT 
GGCAGCACTG GCCATTGTTG GTGTGTGGAC AAATTAGGTG AAGAGATTAC TGGAACAAGA 
ACAACACCGG GCAGACCAGC CCCAAATTGT GGGGAAACAG AGCCTACTCG GAGACCTCAG 
ACAGTGTGTG AGAGGTGGAA GCAGAGTCTT ATTGAGCATT ATGGTGGGAA ACCAGGTGGA 
GAACATTATG TGCCTCAGTG TGATCAATAT GGTGACTTCA GTCCTCTCCA GTGCCATGGA 
AACAGTGGCT ATTGCTGGTG TGTGGATAAA GAAGGAAGAG AAATTGAAGG ATCTAGAACA 
GAACCTGGAA TGACTCCAGC ATGTATACCG ACAGTGGCAC CTCCAACCAT GCATCCAACT 
CCGCGTCCTG ATGTGACCCC TCCTGCAACT GGCACCTTCC TCTTGTATGC TCAGGGTCAG 
CAGATTGGCT ACTTACCACT CAACGGCACA AGGCTCCATA AGGAAAAAGC CAGGACACTG 
CTGTCACTTC ATGGTTCTAT AGTGGTTGGT ATTGACTATG ACTGCCATGA GAAAATGGTG 
TACTGGACGG ATGTTGCAGG TCGTACAATC AACCGCGCAA GCTTAGAACC AGGTGCAGAA 
CCGGAAACTA TCATTAACAC AGGGTTGATG AGCACAGAGG GCTTAGCCAT TGACTACCTT 
CGCAGGACAA TGTTTTGGAC AGATAGCGGC CTAGACAAGA TTGAGAGTGC GCGGTTGGAC 
GGCACTGAGA GGAAGGTTCT ATTTGACACA CAGCTTGTGA ATCCTCGAGC CATCACTGTG 
GATGCGGTTA GAGGGAACCT GTACTGGACA GACTGGAACA GAGAGGCTCC AAAGATTGAA 
TCTTCGTTTG TAGACGGATC AAACAGGAGG ATTCTGGTTA ATGACAACAT CGGCCTTCCC 
AACGGTTTAA CCTTTGATCC CTTCTCCAAA CAAGTCTGTT GGGCTGATGC AGGAACCAAG 
AAGCTTGAAT GCATTTTGTC AGACGGAACA GGGCGACGGG TCATACAGAG TAACTTGAAT 
TATCCTTTTA GTGTTGTTGT GTATGCAAAT TACTTCTACC ACACAGACTG GAGGAGGGAT 
GGGGTAATAT CCCTTAACAA GGACACTGGG CACATTGTTG AAGAATACCT TCCTGAGCAG 
CGATCCCATC TCTATGGAGT AACAGCTGTG TACCCATACT GCCCTTCAGG GAGAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012823265.2
CDS	95..4414
Translation	MWDRTLHLLLPLHCLLAFSAAISRHELFPYGELRGDVLLQEGDDETSEVVKLSQPLLFYEAQFSNLYVGTNGIISTQDFPRETQYVDDGFPTDFPVIAPFLSDIDTSNGKGKIFYRQDNSEQVLNRAVSEIQRGFPETSFVPTDAFLVTWENVGAYEEMTRHSPSSDRRNTFQAVLAYDHSDAYAIFLYPEDGLQFFGTRPKESYNVHIELPARVGFSRSESGPYYSVTSNEQSVKNLYQTGNTGVPGIWVFHIGSIWELDNVIPAKFFGAPTKEQSLENSHTGFPEHRIFEKNESEDSIDYPESFYEENEDDLEDNTSDATPTHNAHQKVDSDTGNQGYLDIDQASKLTHADVEQEATSSVVLSSSHQNPDWTPYKLSESDTDRDGPLQEPQVQTGEDTDLTRGDTLLTEELPSHKAHNLTPQNSEDLSIYPESEVLVGSHPEQENDFERKQIDADPGLQTDVFSFNPVGKETCERNHGRCSRFAFCADYSAGFCCHCQEDYYGNGVHCLPRGAPHRVNGKVSGNILVGQTPVNFIAADLHAYIVANDGRAYTAISHVPDPASWSLTPLAPIGGLFGWLFALEKPGFQNGFSFTGAKFVHNIDVTFYPGEETVHITQTADGFDSEGYMNVKTAIQGKIPYIPETSTIKLSPYTELYQYSGSVVTSTAYREYTVISESNEEQKLSYRLRQNVTYQECSHSQKQPVSAQKLYVDRVFALYNKEEKVLRYAVTNYIGSLHDSTGQDDLTVNPCYDGTHLCDTRAKCQPGSGLEYSCVCASGYQGDGRDCTDVNECEVGFTRCGQNTVCVNLQGSYRCECASGFTLSGDGHNCILASLINPCEDGSHTCNRDTSRCVPRGDGVFTCECFPGFNKSGEDCVDVDECTEHRCHPDASCTNTLGSFSCRCNSGYEGDGFQCTQILGPEPLKTPCLEKRQQLHPRGPRPAVGQFVPECDVEGNYVPLQCHGSTGHCWCVDKLGEEITGTRTPPGRPAPNCGERGPEPLKTPCLEKRQQLHPRGPRPAVGQFVPECDVEGNYVPLQCHGSTGHCWCVDKLGEEITGTRTTPGRPAPNCGETEPTRRPQTVCERWKQSLIEHYGGKPGGEHYVPQCDQYGDFSPLQCHGNSGYCWCVDKEGREIEGSRTEPGMTPACIPTVAPPTMHPTPRPDVTPPATGTFLLYAQGQQIGYLPLNGTRLHKEKARTLLSLHGSIVVGIDYDCHEKMVYWTDVAGRTINRASLEPGAEPETIINTGLMSTEGLAIDYLRRTMFWTDSGLDKIESARLDGTERKVLFDTQLVNPRAITVDAVRGNLYWTDWNREAPKIESSFVDGSNRRILVNDNIGLPNGLTFDPFSKQVCWADAGTKKLECILSDGTGRRVIQSNLNYPFSVVVYANYFYHTDWRRDGVISLNKDTGHIVEEYLPEQRSHLYGVTAVYPYCPSGRK

Target ORF information:

RefSeq Version	XM_012967811.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nidogen 2 (nid2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967811.3

ORF Insert Sequence:

ATGTGGGACA GAACTCTGCA TCTGCTCCTG CCCTTGCACT GCCTGCTGGC CTTCTCTGCT 
GCTATATCCC GGCATGAACT CTTCCCCTAT GGGGAACTGA GGGGAGATGT GCTCCTGCAG 
GAGGGGGACG ATGAGACTTC TGAGGTGGTG AAACTCTCCC AACCGCTGCT CTTCTATGAA 
GCCCAATTCA GCAATTTATA TGTAGGGACC AATGGAATTA TTTCCACTCA GGACTTCCCC 
AGAGAAACTC AGTATGTGGA TGATGGCTTC CCTACAGACT TCCCTGTCAT TGCTCCATTC 
CTCTCAGATA TAGATACCAG CAATGGCAAA GGAAAGATTT TTTATCGGCA GGACAATTCT 
GAACAAGTTC TCAATCGGGC AGTCAGTGAG ATCCAGAGGG GTTTCCCAGA GACTTCATTT 
GTTCCTACTG ATGCCTTCCT TGTGACTTGG GAAAATGTTG GTGCCTACGA AGAGATGACC 
CGTCACTCTC CATCGTCTGA TAGGAGAAAC ACGTTCCAGG CGGTACTAGC ATATGATCAC 
TCTGATGCTT ACGCTATATT CCTTTATCCT GAAGATGGAC TCCAGTTTTT TGGTACTCGT 
CCTAAGGAAT CCTACAATGT GCACATTGAG CTGCCTGCCA GAGTGGGCTT TAGCCGATCA 
GAATCGGGAC CATACTACAG TGTAACAAGT AATGAGCAAT CCGTGAAGAA TTTGTATCAA 
ACTGGAAACA CTGGTGTCCC AGGGATTTGG GTCTTTCACA TTGGCAGCAT TTGGGAATTA 
GACAATGTAA TTCCAGCAAA ATTCTTTGGT GCTCCCACAA AGGAACAATC GCTTGAAAAT 
TCACATACAG GTTTCCCAGA ACACAGAATA TTTGAAAAGA ATGAAAGTGA AGACAGTATT 
GATTACCCAG AGTCCTTTTA TGAGGAGAAT GAAGATGATC TGGAAGATAA TACAAGTGAT 
GCCACACCAA CACATAATGC ACACCAAAAA GTTGATTCAG ACACTGGAAA TCAGGGATAC 
TTGGACATAG ATCAGGCTTC CAAACTGACC CATGCAGATG TTGAACAGGA AGCTACATCA 
TCTGTTGTGC TTTCCTCTTC ACATCAGAAT CCTGATTGGA CACCCTACAA ACTCAGTGAA 
TCTGATACTG ACAGAGATGG GCCATTGCAG GAACCTCAAG TTCAGACAGG GGAGGATACA 
GATTTGACAA GGGGAGACAC ATTACTGACT GAAGAGCTGC CATCCCATAA AGCACATAAT 
TTAACACCTC AAAATTCTGA AGATCTCTCA ATATATCCTG AAAGTGAAGT TCTCGTGGGC 
AGTCACCCAG AACAGGAAAA TGATTTTGAA AGAAAGCAGA TTGATGCTGA TCCTGGACTC 
CAAACTGATG TGTTTTCCTT TAACCCTGTG GGCAAGGAGA CATGTGAGAG GAACCACGGG 
AGATGTTCTA GGTTTGCTTT TTGTGCCGAT TACTCTGCTG GCTTCTGCTG TCATTGCCAG 
GAAGATTATT ATGGCAACGG AGTACATTGT TTGCCAAGAG GGGCTCCACA CCGTGTGAAT 
GGTAAAGTGA GCGGAAACAT CCTGGTCGGT CAAACACCTG TCAACTTCAT TGCTGCCGAT 
TTACACGCAT ACATTGTGGC AAATGATGGA AGAGCGTACA CAGCAATCAG CCACGTTCCT 
GATCCTGCAT CCTGGTCTTT AACACCTCTT GCTCCAATTG GGGGACTCTT TGGCTGGCTT 
TTTGCTTTGG AAAAACCAGG CTTTCAAAAT GGTTTTAGCT TTACCGGTGC CAAGTTTGTA 
CACAACATTG ATGTCACATT TTACCCGGGA GAAGAAACTG TTCATATTAC TCAAACAGCA 
GATGGCTTTG ATTCTGAGGG TTATATGAAT GTAAAGACAG CAATTCAGGG CAAAATTCCA 
TATATCCCAG AGACATCGAC CATCAAGCTG TCTCCTTATA CTGAACTGTA TCAATACTCT 
GGCTCAGTTG TAACTTCGAC AGCCTACAGG GAATACACAG TCATCTCAGA ATCAAACGAA 
GAACAAAAAC TGTCTTACCG GTTACGGCAA AATGTCACTT ACCAAGAGTG CAGCCATTCC 
CAGAAACAGC CTGTTAGTGC TCAAAAGCTT TATGTTGATC GTGTCTTTGC CCTTTACAAT 
AAAGAAGAGA AGGTCCTTCG TTATGCAGTT ACAAACTACA TTGGATCTCT GCATGATTCG 
ACTGGACAGG ACGATCTCAC AGTTAACCCG TGCTATGATG GTACCCACTT GTGTGACACA 
AGAGCTAAAT GCCAGCCTGG TAGTGGTCTA GAATACAGTT GTGTTTGTGC GTCTGGATAC 
CAAGGGGATG GAAGAGATTG CACTGATGTA AATGAATGTG AAGTTGGCTT CACCCGGTGC 
GGCCAGAACA CTGTTTGTGT CAACCTCCAG GGGAGTTACC GTTGTGAGTG CGCCAGTGGT 
TTCACTTTAT CTGGAGACGG GCACAACTGC ATCTTGGCTT CACTAATAAA CCCATGTGAA 
GATGGAAGTC ATACATGTAA CAGAGACACA TCACGCTGTG TTCCGCGTGG AGATGGTGTA 
TTCACATGTG AATGTTTCCC TGGATTTAAC AAGAGTGGAG AAGACTGTGT TGATGTTGAT 
GAATGCACTG AGCACAGATG TCATCCAGAT GCCTCATGTA CCAACACCCT GGGCTCTTTC 
TCCTGTCGGT GTAACAGTGG TTATGAAGGA GATGGCTTCC AGTGCACTCA AATCTTAGGT 
CCTGAACCCC TGAAAACCCC CTGTTTAGAA AAGCGCCAGC AGCTACACCC AAGGGGCCCC 
CGTCCAGCTG TGGGACAGTT TGTACCAGAG TGTGATGTAG AGGGAAATTA TGTTCCATTA 
CAGTGTCATG GCAGCACTGG CCATTGTTGG TGTGTGGACA AATTAGGTGA AGAGATTACT 
GGAACAAGAA CACCACCGGG CAGACCAGCC CCAAATTGTG GGGAAAGAGG TCCTGAACCC 
CTGAAAACCC CCTGTTTAGA AAAGCGCCAG CAGCTACACC CAAGGGGCCC CCGTCCAGCT 
GTGGGACAGT TTGTACCAGA GTGTGATGTA GAGGGAAATT ATGTTCCATT ACAGTGTCAT 
GGCAGCACTG GCCATTGTTG GTGTGTGGAC AAATTAGGTG AAGAGATTAC TGGAACAAGA 
ACAACACCGG GCAGACCAGC CCCAAATTGT GGGGAAACAG AGCCTACTCG GAGACCTCAG 
ACAGTGTGTG AGAGGTGGAA GCAGAGTCTT ATTGAGCATT ATGGTGGGAA ACCAGGTGGA 
GAACATTATG TGCCTCAGTG TGATCAATAT GGTGACTTCA GTCCTCTCCA GTGCCATGGA 
AACAGTGGCT ATTGCTGGTG TGTGGATAAA GAAGGAAGAG AAATTGAAGG ATCTAGAACA 
GAACCTGGAA TGACTCCAGC ATGTATACCG ACAGTGGCAC CTCCAACCAT GCATCCAACT 
CCGCGTCCTG ATGTGACCCC TCCTGCAACT GGCACCTTCC TCTTGTATGC TCAGGGTCAG 
CAGATTGGCT ACTTACCACT CAACGGCACA AGGCTCCATA AGGAAAAAGC CAGGACACTG 
CTGTCACTTC ATGGTTCTAT AGTGGTTGGT ATTGACTATG ACTGCCATGA GAAAATGGTG 
TACTGGACGG ATGTTGCAGG TCGTACAATC AACCGCGCAA GCTTAGAACC AGGTGCAGAA 
CCGGAAACTA TCATTAACAC AGGGTTGATG AGCACAGAGG GCTTAGCCAT TGACTACCTT 
CGCAGGACAA TGTTTTGGAC AGATAGCGGC CTAGACAAGA TTGAGAGTGC GCGGTTGGAC 
GGCACTGAGA GGAAGGTTCT ATTTGACACA CAGCTTGTGA ATCCTCGAGC CATCACTGTG 
GATGCGGTTA GAGGGAACCT GTACTGGACA GACTGGAACA GAGAGGCTCC AAAGATTGAA 
TCTTCGTTTG TAGACGGATC AAACAGGAGG ATTCTGGTTA ATGACAACAT CGGCCTTCCC 
AACGGTTTAA CCTTTGATCC CTTCTCCAAA CAAGTCTGTT GGGCTGATGC AGGAACCAAG 
AAGCTTGAAT GCATTTTGTC AGACGGAACA GGGCGACGGG TCATACAGAG TAACTTGAAT 
TATCCTTTTA GTGTTGTTGT GTATGCAAAT TACTTCTACC ACACAGACTG GAGGAGGGAT 
GGGGTAATAT CCCTTAACAA GGACACTGGG CACATTGTTG AAGAATACCT TCCTGAGCAG 
CGATCCCATC TCTATGGAGT AACAGCTGTG TACCCATACT GCCCTTCAGG GAGAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nid2 ( XM_012967809.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823263.2 Xenopus tropicalis nidogen 2 (nid2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa63346
Clone ID Related Accession (Same CDS sequence)	XM_012967809.3
Accession Version	XM_012967809.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nidogen-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012967809.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGACA GAACTCTGCA TCTGCTCCTG CCCTTGCACT GCCTGCTGGC CTTCTCTGCT 
GCTATATCCC GGCATGAACT CTTCCCCTAT GGGGAACTGA GGGGAGATGT GCTCCTGCAG 
GAGGGGGACG ATGAGACTTC TGAGGTGGTG AAACTCTCCC AACCGCTGCT CTTCTATGAA 
GCCCAATTCA GCAATTTATA TGTAGGGACC AATGGAATTA TTTCCACTCA GGACTTCCCC 
AGAGAAACTC AGTATGTGGA TGATGGCTTC CCTACAGACT TCCCTGTCAT TGCTCCATTC 
CTCTCAGATA TAGATACCAG CAATGGCAAA GGAAAGATTT TTTATCGGCA GGACAATTCT 
GAACAAGTTC TCAATCGGGC AGTCAGTGAG ATCCAGAGGG GTTTCCCAGA GACTTCATTT 
GTTCCTACTG ATGCCTTCCT TGTGACTTGG GAAAATGTTG GTGCCTACGA AGAGATGACC 
CGTCACTCTC CATCGTCTGA TAGGAGAAAC ACGTTCCAGG CGGTACTAGC ATATGATCAC 
TCTGATGCTT ACGCTATATT CCTTTATCCT GAAGATGGAC TCCAGTTTTT TGGTACTCGT 
CCTAAGGAAT CCTACAATGT GCACATTGAG CTGCCTGCCA GAGTGGGCTT TAGCCGATCA 
GAATCGGGAC CATACTACAG TGTAACAAGT AATGAGCAAT CCGTGAAGAA TTTGTATCAA 
ACTGGAAACA CTGGTGTCCC AGGGATTTGG GTCTTTCACA TTGGCAGCAT TTGGGAATTA 
GACAATGTAA TTCCAGCAAA ATTCTTTGGT GCTCCCACAA AGGAACAATC GCTTGAAAAT 
TCACATACAG GTTTCCCAGA ACACAGAATA TTTGAAAAGA ATGAAAGTGA AGACAGTATT 
GATTACCCAG AGTCCTTTTA TGAGGAGAAT GAAGATGATC TGGAAGATAA TACAAGTGAT 
GCCACACCAA CACATAATGC ACACCAAAAA GTTGATTCAG ACACTGGAAA TCAGGGATAC 
TTGGACATAG ATCAGGCTTC CAAACTGACC CATGCAGATG TTGAACAGGA AGCTACATCA 
TCTGTTGTGC TTTCCTCTTC ACATCAGAAT CCTGATTGGA CACCCTACAA ACTCAGTGAA 
TCTGATACTG ACAGAGATGG GCCATTGCAG GAACCTCAAG TTCAGACAGG GGAGGATACA 
GATTTGACAA GGGGAGACAC ATTACTGACT GAAGAGCTGC CATCCCATAA AGCACATAAT 
TTAACACCTC AAAATTCTGA AGATCTCTCA ATATATCCTG AAAGTGAAGT TCTCGTGGGC 
AGTCACCCAG AACAGGAAAA TGATTTTGAA AGAAAGCAGA TTGATGCTGA TCCTGGACTC 
CAAACTGATG TGTTTTCCTT TAACCCTGTG GGCAAGGAGA CATGTGAGAG GAACCACGGG 
AGATGTTCTA GGTTTGCTTT TTGTGCCGAT TACTCTGCTG GCTTCTGCTG TCATTGCCAG 
GAAGATTATT ATGGCAACGG AGTACATTGT TTGCCAAGAG GGGCTCCACA CCGTGTGAAT 
GGTAAAGTGA GCGGAAACAT CCTGGTCGGT CAAACACCTG TCAACTTCAT TGCTGCCGAT 
TTACACGCAT ACATTGTGGC AAATGATGGA AGAGCGTACA CAGCAATCAG CCACGTTCCT 
GATCCTGCAT CCTGGTCTTT AACACCTCTT GCTCCAATTG GGGGACTCTT TGGCTGGCTT 
TTTGCTTTGG AAAAACCAGG CTTTCAAAAT GGTTTTAGCT TTACCGGTGC CAAGTTTGTA 
CACAACATTG ATGTCACATT TTACCCGGGA GAAGAAACTG TTCATATTAC TCAAACAGCA 
GATGGCTTTG ATTCTGAGGG TTATATGAAT GTAAAGACAG CAATTCAGGG CAAAATTCCA 
TATATCCCAG AGACATCGAC CATCAAGCTG TCTCCTTATA CTGAACTGTA TCAATACTCT 
GGCTCAGTTG TAACTTCGAC AGCCTACAGG GAATACACAG TCATCTCAGA ATCAAACGAA 
GAACAAAAAC TGTCTTACCG GTTACGGCAA AATGTCACTT ACCAAGAGTG CAGCCATTCC 
CAGAAACAGC CTGTTAGTGC TCAAAAGCTT TATGTTGATC GTGTCTTTGC CCTTTACAAT 
AAAGAAGAGA AGGTCCTTCG TTATGCAGTT ACAAACTACA TTGGATCTCT GCATGATTCG 
ACTGGACAGG ACGATCTCAC AGTTAACCCG TGCTATGATG GTACCCACTT GTGTGACACA 
AGAGCTAAAT GCCAGCCTGG TAGTGGTCTA GAATACAGTT GTGTTTGTGC GTCTGGATAC 
CAAGGGGATG GAAGAGATTG CACTGATGTA AATGAATGTG AAGTTGGCTT CACCCGGTGC 
GGCCAGAACA CTGTTTGTGT CAACCTCCAG GGGAGTTACC GTTGTGAGTG CGCCAGTGGT 
TTCACTTTAT CTGGAGACGG GCACAACTGC ATCTTGGCTT CACTAATAAA CCCATGTGAA 
GATGGAAGTC ATACATGTAA CAGAGACACA TCACGCTGTG TTCCGCGTGG AGATGGTGTA 
TTCACATGTG AATGTTTCCC TGGATTTAAC AAGAGTGGAG AAGACTGTGT TGATGTTGAT 
GAATGCACTG AGCACAGATG TCATCCAGAT GCCTCATGTA CCAACACCCT GGGCTCTTTC 
TCCTGTCGGT GTAACAGTGG TTATGAAGGA GATGGCTTCC AGTGCACTCA AATCTTAGGT 
CCTGAACCCC TGAAAACCCC CTGTTTAGAA AAGCGCCAGC AGCTACACCC AAGGGGCCCC 
CGTCCAGCTG TGGGACAGTT TGTACCAGAG TGTGATGTAG AGGGAAATTA TGTTCCATTA 
CAGTGTCATG GCAGCACTGG CCATTGTTGG TGTGTGGACA AATTAGGTGA AGAGATTACT 
GGAACAAGAA CACCACCGGG CAGACCAGCC CCAAATTGTG GGGAAAGAGG TCCTGAACCC 
CTGAAAACCC CCTGTTTAGA AAAGCGCCAG CAGCTACACC CAAGGGGCCC CCGTCCAGCT 
GTGGGACAGT TTGTACCAGA GTGTGATGTA GAGGGAAATT ATGTTCCATT ACAGTGTCAT 
GGCAGCACTG GCCATTGTTG GTGTGTGGAC AAATTAGGTG AAGAGATTAC TGGAACAAGA 
ACAACACCGG GCAGACCAGC CCCAAATTGT GGGGAAACAG GTCCTGAACC CCTGAAAACC 
CCCTGTTTAG AAAGGCGCCA GCAGCTGCTT GGACAGCTGC ACCCAAGAGG CCCCCGTCCA 
GCTGTGGGAC AGTTTGTACC TGAGTGTGAT GCAGAGGGAA ATTATGTTTC ATTACAGTGT 
CATGGCAGCA CTGGCCATTG TTGGTGTGTG GACAAATTAG GTGAAGAGAT TGCTGGAACA 
AGAACACCAC CTGGCAGACC AGGCCCAAAT TGTGGGGAAA CAGCAGAGCC TACTCGGAGA 
CCTCAGACAG TGTGTGAGAG GTGGAAGCAG AGTCTTATTG AGCATTATGG TGGGAAACCA 
GGTGGAGAAC ATTATGTGCC TCAGTGTGAT CAATATGGTG ACTTCAGTCC TCTCCAGTGC 
CATGGAAACA GTGGCTATTG CTGGTGTGTG GATAAAGAAG GAAGAGAAAT TGAAGGATCT 
AGAACAGAAC CTGGAATGAC TCCAGCATGT ATACCGACAG TGGCACCTCC AACCATGCAT 
CCAACTCCGC GTCCTGATGT GACCCCTCCT GCAACTGGCA CCTTCCTCTT GTATGCTCAG 
GGTCAGCAGA TTGGCTACTT ACCACTCAAC GGCACAAGGC TCCATAAGGA AAAAGCCAGG 
ACACTGCTGT CACTTCATGG TTCTATAGTG GTTGGTATTG ACTATGACTG CCATGAGAAA 
ATGGTGTACT GGACGGATGT TGCAGGTCGT ACAATCAACC GCGCAAGCTT AGAACCAGGT 
GCAGAACCGG AAACTATCAT TAACACAGGG TTGATGAGCA CAGAGGGCTT AGCCATTGAC 
TACCTTCGCA GGACAATGTT TTGGACAGAT AGCGGCCTAG ACAAGATTGA GAGTGCGCGG 
TTGGACGGCA CTGAGAGGAA GGTTCTATTT GACACACAGC TTGTGAATCC TCGAGCCATC 
ACTGTGGATG CGGTTAGAGG GAACCTGTAC TGGACAGACT GGAACAGAGA GGCTCCAAAG 
ATTGAATCTT CGTTTGTAGA CGGATCAAAC AGGAGGATTC TGGTTAATGA CAACATCGGC 
CTTCCCAACG GTTTAACCTT TGATCCCTTC TCCAAACAAG TCTGTTGGGC TGATGCAGGA 
ACCAAGAAGC TTGAATGCAT TTTGTCAGAC GGAACAGGGC GACGGGTCAT ACAGAGTAAC 
TTGAATTATC CTTTTAGTGT TGTTGTGTAT GCAAATTACT TCTACCACAC AGACTGGAGG 
AGGGATGGGG TAATATCCCT TAACAAGGAC ACTGGGCACA TTGTTGAAGA ATACCTTCCT 
GAGCAGCGAT CCCATCTCTA TGGAGTAACA GCTGTGTACC CATACTGCCC TTCAGGGAGA 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012823263.2
CDS	95..4660
Translation	MWDRTLHLLLPLHCLLAFSAAISRHELFPYGELRGDVLLQEGDDETSEVVKLSQPLLFYEAQFSNLYVGTNGIISTQDFPRETQYVDDGFPTDFPVIAPFLSDIDTSNGKGKIFYRQDNSEQVLNRAVSEIQRGFPETSFVPTDAFLVTWENVGAYEEMTRHSPSSDRRNTFQAVLAYDHSDAYAIFLYPEDGLQFFGTRPKESYNVHIELPARVGFSRSESGPYYSVTSNEQSVKNLYQTGNTGVPGIWVFHIGSIWELDNVIPAKFFGAPTKEQSLENSHTGFPEHRIFEKNESEDSIDYPESFYEENEDDLEDNTSDATPTHNAHQKVDSDTGNQGYLDIDQASKLTHADVEQEATSSVVLSSSHQNPDWTPYKLSESDTDRDGPLQEPQVQTGEDTDLTRGDTLLTEELPSHKAHNLTPQNSEDLSIYPESEVLVGSHPEQENDFERKQIDADPGLQTDVFSFNPVGKETCERNHGRCSRFAFCADYSAGFCCHCQEDYYGNGVHCLPRGAPHRVNGKVSGNILVGQTPVNFIAADLHAYIVANDGRAYTAISHVPDPASWSLTPLAPIGGLFGWLFALEKPGFQNGFSFTGAKFVHNIDVTFYPGEETVHITQTADGFDSEGYMNVKTAIQGKIPYIPETSTIKLSPYTELYQYSGSVVTSTAYREYTVISESNEEQKLSYRLRQNVTYQECSHSQKQPVSAQKLYVDRVFALYNKEEKVLRYAVTNYIGSLHDSTGQDDLTVNPCYDGTHLCDTRAKCQPGSGLEYSCVCASGYQGDGRDCTDVNECEVGFTRCGQNTVCVNLQGSYRCECASGFTLSGDGHNCILASLINPCEDGSHTCNRDTSRCVPRGDGVFTCECFPGFNKSGEDCVDVDECTEHRCHPDASCTNTLGSFSCRCNSGYEGDGFQCTQILGPEPLKTPCLEKRQQLHPRGPRPAVGQFVPECDVEGNYVPLQCHGSTGHCWCVDKLGEEITGTRTPPGRPAPNCGERGPEPLKTPCLEKRQQLHPRGPRPAVGQFVPECDVEGNYVPLQCHGSTGHCWCVDKLGEEITGTRTTPGRPAPNCGETGPEPLKTPCLERRQQLLGQLHPRGPRPAVGQFVPECDAEGNYVSLQCHGSTGHCWCVDKLGEEIAGTRTPPGRPGPNCGETAEPTRRPQTVCERWKQSLIEHYGGKPGGEHYVPQCDQYGDFSPLQCHGNSGYCWCVDKEGREIEGSRTEPGMTPACIPTVAPPTMHPTPRPDVTPPATGTFLLYAQGQQIGYLPLNGTRLHKEKARTLLSLHGSIVVGIDYDCHEKMVYWTDVAGRTINRASLEPGAEPETIINTGLMSTEGLAIDYLRRTMFWTDSGLDKIESARLDGTERKVLFDTQLVNPRAITVDAVRGNLYWTDWNREAPKIESSFVDGSNRRILVNDNIGLPNGLTFDPFSKQVCWADAGTKKLECILSDGTGRRVIQSNLNYPFSVVVYANYFYHTDWRRDGVISLNKDTGHIVEEYLPEQRSHLYGVTAVYPYCPSGRK

Target ORF information:

RefSeq Version	XM_012967809.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nidogen 2 (nid2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967809.3

ORF Insert Sequence:

ATGTGGGACA GAACTCTGCA TCTGCTCCTG CCCTTGCACT GCCTGCTGGC CTTCTCTGCT 
GCTATATCCC GGCATGAACT CTTCCCCTAT GGGGAACTGA GGGGAGATGT GCTCCTGCAG 
GAGGGGGACG ATGAGACTTC TGAGGTGGTG AAACTCTCCC AACCGCTGCT CTTCTATGAA 
GCCCAATTCA GCAATTTATA TGTAGGGACC AATGGAATTA TTTCCACTCA GGACTTCCCC 
AGAGAAACTC AGTATGTGGA TGATGGCTTC CCTACAGACT TCCCTGTCAT TGCTCCATTC 
CTCTCAGATA TAGATACCAG CAATGGCAAA GGAAAGATTT TTTATCGGCA GGACAATTCT 
GAACAAGTTC TCAATCGGGC AGTCAGTGAG ATCCAGAGGG GTTTCCCAGA GACTTCATTT 
GTTCCTACTG ATGCCTTCCT TGTGACTTGG GAAAATGTTG GTGCCTACGA AGAGATGACC 
CGTCACTCTC CATCGTCTGA TAGGAGAAAC ACGTTCCAGG CGGTACTAGC ATATGATCAC 
TCTGATGCTT ACGCTATATT CCTTTATCCT GAAGATGGAC TCCAGTTTTT TGGTACTCGT 
CCTAAGGAAT CCTACAATGT GCACATTGAG CTGCCTGCCA GAGTGGGCTT TAGCCGATCA 
GAATCGGGAC CATACTACAG TGTAACAAGT AATGAGCAAT CCGTGAAGAA TTTGTATCAA 
ACTGGAAACA CTGGTGTCCC AGGGATTTGG GTCTTTCACA TTGGCAGCAT TTGGGAATTA 
GACAATGTAA TTCCAGCAAA ATTCTTTGGT GCTCCCACAA AGGAACAATC GCTTGAAAAT 
TCACATACAG GTTTCCCAGA ACACAGAATA TTTGAAAAGA ATGAAAGTGA AGACAGTATT 
GATTACCCAG AGTCCTTTTA TGAGGAGAAT GAAGATGATC TGGAAGATAA TACAAGTGAT 
GCCACACCAA CACATAATGC ACACCAAAAA GTTGATTCAG ACACTGGAAA TCAGGGATAC 
TTGGACATAG ATCAGGCTTC CAAACTGACC CATGCAGATG TTGAACAGGA AGCTACATCA 
TCTGTTGTGC TTTCCTCTTC ACATCAGAAT CCTGATTGGA CACCCTACAA ACTCAGTGAA 
TCTGATACTG ACAGAGATGG GCCATTGCAG GAACCTCAAG TTCAGACAGG GGAGGATACA 
GATTTGACAA GGGGAGACAC ATTACTGACT GAAGAGCTGC CATCCCATAA AGCACATAAT 
TTAACACCTC AAAATTCTGA AGATCTCTCA ATATATCCTG AAAGTGAAGT TCTCGTGGGC 
AGTCACCCAG AACAGGAAAA TGATTTTGAA AGAAAGCAGA TTGATGCTGA TCCTGGACTC 
CAAACTGATG TGTTTTCCTT TAACCCTGTG GGCAAGGAGA CATGTGAGAG GAACCACGGG 
AGATGTTCTA GGTTTGCTTT TTGTGCCGAT TACTCTGCTG GCTTCTGCTG TCATTGCCAG 
GAAGATTATT ATGGCAACGG AGTACATTGT TTGCCAAGAG GGGCTCCACA CCGTGTGAAT 
GGTAAAGTGA GCGGAAACAT CCTGGTCGGT CAAACACCTG TCAACTTCAT TGCTGCCGAT 
TTACACGCAT ACATTGTGGC AAATGATGGA AGAGCGTACA CAGCAATCAG CCACGTTCCT 
GATCCTGCAT CCTGGTCTTT AACACCTCTT GCTCCAATTG GGGGACTCTT TGGCTGGCTT 
TTTGCTTTGG AAAAACCAGG CTTTCAAAAT GGTTTTAGCT TTACCGGTGC CAAGTTTGTA 
CACAACATTG ATGTCACATT TTACCCGGGA GAAGAAACTG TTCATATTAC TCAAACAGCA 
GATGGCTTTG ATTCTGAGGG TTATATGAAT GTAAAGACAG CAATTCAGGG CAAAATTCCA 
TATATCCCAG AGACATCGAC CATCAAGCTG TCTCCTTATA CTGAACTGTA TCAATACTCT 
GGCTCAGTTG TAACTTCGAC AGCCTACAGG GAATACACAG TCATCTCAGA ATCAAACGAA 
GAACAAAAAC TGTCTTACCG GTTACGGCAA AATGTCACTT ACCAAGAGTG CAGCCATTCC 
CAGAAACAGC CTGTTAGTGC TCAAAAGCTT TATGTTGATC GTGTCTTTGC CCTTTACAAT 
AAAGAAGAGA AGGTCCTTCG TTATGCAGTT ACAAACTACA TTGGATCTCT GCATGATTCG 
ACTGGACAGG ACGATCTCAC AGTTAACCCG TGCTATGATG GTACCCACTT GTGTGACACA 
AGAGCTAAAT GCCAGCCTGG TAGTGGTCTA GAATACAGTT GTGTTTGTGC GTCTGGATAC 
CAAGGGGATG GAAGAGATTG CACTGATGTA AATGAATGTG AAGTTGGCTT CACCCGGTGC 
GGCCAGAACA CTGTTTGTGT CAACCTCCAG GGGAGTTACC GTTGTGAGTG CGCCAGTGGT 
TTCACTTTAT CTGGAGACGG GCACAACTGC ATCTTGGCTT CACTAATAAA CCCATGTGAA 
GATGGAAGTC ATACATGTAA CAGAGACACA TCACGCTGTG TTCCGCGTGG AGATGGTGTA 
TTCACATGTG AATGTTTCCC TGGATTTAAC AAGAGTGGAG AAGACTGTGT TGATGTTGAT 
GAATGCACTG AGCACAGATG TCATCCAGAT GCCTCATGTA CCAACACCCT GGGCTCTTTC 
TCCTGTCGGT GTAACAGTGG TTATGAAGGA GATGGCTTCC AGTGCACTCA AATCTTAGGT 
CCTGAACCCC TGAAAACCCC CTGTTTAGAA AAGCGCCAGC AGCTACACCC AAGGGGCCCC 
CGTCCAGCTG TGGGACAGTT TGTACCAGAG TGTGATGTAG AGGGAAATTA TGTTCCATTA 
CAGTGTCATG GCAGCACTGG CCATTGTTGG TGTGTGGACA AATTAGGTGA AGAGATTACT 
GGAACAAGAA CACCACCGGG CAGACCAGCC CCAAATTGTG GGGAAAGAGG TCCTGAACCC 
CTGAAAACCC CCTGTTTAGA AAAGCGCCAG CAGCTACACC CAAGGGGCCC CCGTCCAGCT 
GTGGGACAGT TTGTACCAGA GTGTGATGTA GAGGGAAATT ATGTTCCATT ACAGTGTCAT 
GGCAGCACTG GCCATTGTTG GTGTGTGGAC AAATTAGGTG AAGAGATTAC TGGAACAAGA 
ACAACACCGG GCAGACCAGC CCCAAATTGT GGGGAAACAG GTCCTGAACC CCTGAAAACC 
CCCTGTTTAG AAAGGCGCCA GCAGCTGCTT GGACAGCTGC ACCCAAGAGG CCCCCGTCCA 
GCTGTGGGAC AGTTTGTACC TGAGTGTGAT GCAGAGGGAA ATTATGTTTC ATTACAGTGT 
CATGGCAGCA CTGGCCATTG TTGGTGTGTG GACAAATTAG GTGAAGAGAT TGCTGGAACA 
AGAACACCAC CTGGCAGACC AGGCCCAAAT TGTGGGGAAA CAGCAGAGCC TACTCGGAGA 
CCTCAGACAG TGTGTGAGAG GTGGAAGCAG AGTCTTATTG AGCATTATGG TGGGAAACCA 
GGTGGAGAAC ATTATGTGCC TCAGTGTGAT CAATATGGTG ACTTCAGTCC TCTCCAGTGC 
CATGGAAACA GTGGCTATTG CTGGTGTGTG GATAAAGAAG GAAGAGAAAT TGAAGGATCT 
AGAACAGAAC CTGGAATGAC TCCAGCATGT ATACCGACAG TGGCACCTCC AACCATGCAT 
CCAACTCCGC GTCCTGATGT GACCCCTCCT GCAACTGGCA CCTTCCTCTT GTATGCTCAG 
GGTCAGCAGA TTGGCTACTT ACCACTCAAC GGCACAAGGC TCCATAAGGA AAAAGCCAGG 
ACACTGCTGT CACTTCATGG TTCTATAGTG GTTGGTATTG ACTATGACTG CCATGAGAAA 
ATGGTGTACT GGACGGATGT TGCAGGTCGT ACAATCAACC GCGCAAGCTT AGAACCAGGT 
GCAGAACCGG AAACTATCAT TAACACAGGG TTGATGAGCA CAGAGGGCTT AGCCATTGAC 
TACCTTCGCA GGACAATGTT TTGGACAGAT AGCGGCCTAG ACAAGATTGA GAGTGCGCGG 
TTGGACGGCA CTGAGAGGAA GGTTCTATTT GACACACAGC TTGTGAATCC TCGAGCCATC 
ACTGTGGATG CGGTTAGAGG GAACCTGTAC TGGACAGACT GGAACAGAGA GGCTCCAAAG 
ATTGAATCTT CGTTTGTAGA CGGATCAAAC AGGAGGATTC TGGTTAATGA CAACATCGGC 
CTTCCCAACG GTTTAACCTT TGATCCCTTC TCCAAACAAG TCTGTTGGGC TGATGCAGGA 
ACCAAGAAGC TTGAATGCAT TTTGTCAGAC GGAACAGGGC GACGGGTCAT ACAGAGTAAC 
TTGAATTATC CTTTTAGTGT TGTTGTGTAT GCAAATTACT TCTACCACAC AGACTGGAGG 
AGGGATGGGG TAATATCCCT TAACAAGGAC ACTGGGCACA TTGTTGAAGA ATACCTTCCT 
GAGCAGCGAT CCCATCTCTA TGGAGTAACA GCTGTGTACC CATACTGCCC TTCAGGGAGA 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nid2 ( NM_001097263.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001090732.1 Xenopus tropicalis nidogen 2 (nid2), mRNA.

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CloneID	OXa23690
Clone ID Related Accession (Same CDS sequence)	NM_001097263.1
Accession Version	NM_001097263.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nidogen-2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC129003.1. ##Evidence-Data-START## Transcript exon combination :: BC129003.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028290, SAMD00028291 [ECO:0000348] ##Evidence-Data-END##

ATGTGGGACA GAACTCTGCA TCTGCTCCTG CCCTTGCACT GCCTGCTGGC CTTCTCTGCT 
GCTATATCCC GGCATGAACT CTTCCCCTAT GGGGAACTGA GGGGAGATGT GCTCCTGCAG 
GAGGGGGACG ATGAGACTTC TGAGGTGGTG AAACTCTCCC AACCGCTGCT CTTCTATGAA 
GCCCAATTCA GCAATTTATA TGTAGGGACC AATGGAATTA TTTCTACTCA GGACTTCCCC 
AGAGAAACTC AGTATGTGGA TGATGGCTTC CCTACAGACT TCCCTGTCAT TTCTCCATTC 
CTCTCAGATA TAGATACCAG CAATGGCAAA GGAAAGATTT TTTATCGGCA GGACAATTCT 
GAACAAGTTC TCAATCGGGC AGTCAGTGAG ATCCAGAGGG GTTTCCCAGA GACTTCATTT 
GTTCCTACTG ATGCCTTCCT TGTGACTTGG GAAAATGTTG GTGCCTACGA AGAGATGACC 
CGTCTCTCTC CATCGTCCGA TAGGAGAAAC ACGTTCCAGG CGGTACTAGC ATATGATCAC 
TCTGATGCTT ACGCTATATT CCTTTATCCT GAAGATGGAC TCCAGTTTTT TGGTACTCGT 
CCTAAGGAAT CCTACAATGT GCACATTGAG CTGCCTGCCA GAGTGGGCTT TAGCCGATCA 
GAATCGGGAC CATACTACAG TGTAACAAGT AATGAGCAAT CCGTGAAGAA TTTGTATCAA 
ACTGGCAACA CTGGTGTCCC AGGGATTTGG GTCTTTCACA TTGGCAGCAT TTGGGAATTA 
GACAATGTAA TTCCAGCAAA ATTCTTTGGT GCTCCCACAA AGGAACAATC GCTTGAAAAT 
TCACATACAG GTTTCCCAGA ACACAGAATA TTTGAAAAGA ATGAAAGTGA AGACAGTATT 
GATTACCCAG AGTCCTTTTA TGAGGAGAAT GAAGATGATC TGGAAGATAA TACAAGTGAT 
GCCACACCAA CACATAATGC ACACCAAAAA GTTGATTCAG ACACTGGAAA TCAGGGATAC 
TTGGACATAG ATCAGGCTTC CAAACTGACC CATGCAGATG TTGAACAGGA AGCTACATCA 
TCTGTTGTGC TTTCCTCTTC ACATCAGAAT CCTGATTGGA CACCCTACAA ACTCAGTGAA 
TCTGATACTG ACAGAGATGG GCCATTGCAG GAACCTCAAG TTCAGACAGG GGAGGATACA 
GATTTGACAA GGGGAGACAC ATTACTGACT GAAGAGCTGC CATCCCATAA AGCACATAAT 
TTAACACCTC AAAATTCTGA AGATCTCTCA ATATATCCTG AAAGTGAAGT TCTCGTGGGC 
AGTCACCCAG AACAGGAAAA TGATTTTGAA AGAAAGCAGA TTGATGCTGA TCCTGGACTC 
CAAACTGATG TGTTTTCCTT TAACCCTGTG GGCAAGGAGA CATGTGAGAG GAACCACGGG 
AGATGTTCTA GGTTTGCTTT TTGTGCCGAT TACTCTGCTG GCTTCTGCTG TCATTGCCAG 
GAAGATTATT ATGGCAACGG AGTACATTGT TTGCCAAGAG GGGCTCCACA CCGTGTGAAT 
GGTAAAGTGA GCGGAAACAT CCTGGTCGGT CAAACACCTG TCAACTTCAT TGCTGCCGAT 
TTACACGCAT ACATTGTGGC AAATGATGGA AGAGCGTACA CAGCAATCAG CCACGTTCCT 
GATCCTGCAT CCTGGTCTTT AACACCTCTT GCTCCAATTG GGGGACTCTT TGGCTGGCTT 
TTTGCTTTGG AAAAACCAGG CTTTCAAAAT GGTTTTAGCT TTACCGGTGC CAAGTTTGTA 
CACAACATTG ATGTCACATT TTACCCGGGA GAAGAAACTG TTCATATTAC TCAAACAGCA 
GATGGCTTTG ATTCTGAGGG TTATATGAAT GTAAAGACAG CAATTCAGGG CAAAATTCCA 
TATATCCCAG AGACATCGAC CATCAAGCTG TCTCCTTATA CTGAACTGTA TCAATACTCT 
GGCTCAGTTG TAACTTCGAC AGCCTACAGG GAATACACAG TCATCTCAGA ATCAAACGAA 
GAACAAAAAC TGTCTTACCG GTTACGGCAA AATGTCACTT ACCAAGAGTG CAGCCATTCC 
CAGAAACAGC CTGTTAGTGC TCAAAAGCTT TATGTTGATC GTGTCTTTGC CCTTTACAAT 
AAAGAAGAGA AGGTCCTTCG TTATGCAGTT ACAAACTACA TTGGATCTCT GCATGATTCG 
ACTGGACAGG ACGATCTCAC AGTTAACCCG TGCTATGATG GTACCCACTT GTGTGACACA 
AGAGCTAAAT GCCAGCCTGG TAGTGGTCTA GAATACAGTT GTGTTTGTGC GTCTGGATAC 
CAAGGGGATG GAAGAGATTG CACTGATGTA AATGAATGTG AAGTTGGCTT CACCCGGTGC 
GGCCAGAACA CTGTTTGTGT CAACCTCCAG GGGAGTTACC GTTGTGAGTG CGCCAGTGGT 
TTCACTTTAT CTGGAGACGG GCACAACTGC ATCTTGGCTT CACTAATAAA CCCATGTGAA 
GATGGAAGTC ATACATGTAA CAGAGACACA TCACGCTGTG TTCCGCGTGG AGATGGTGTA 
TTCACATGTG AATGTTTCCC TGGATTTAAC AAGAGTGGAG AAGACTGTGT TGATGTTGAT 
GAATGCACTG AGCACAGATG TCATCCAGAT GCCTCATGTA CCAACACCCT GGGCTCTTTC 
TCCTGTCGGT GTAACAGTGG TTATGAAGGA GATGGCTTCC AGTGCACTCA AATCTTAGGT 
CCTGAACCCC TGAAAACCCC CTGTTTAGAA AAGCGCCAGC AGCTACACCC AAGGGGCCCC 
CGTCCAGCTG TGGGACAGTT TGTACCAGAG TGTGATGTAG AGGGAAATTA TGTTCCATTA 
CAGTGTCATG GCAGCACTGG CCATTGTTGG TGTGTGGACA AATTAGGTGA AGAGATTGCT 
GGAACAAGAA CACCACCGGG CAGACCAGCC CCAAATTGTG GGGAAACAGG TCCTGAACCC 
CTGAAAACCC CCTGTTTAGA AAAGCGCCAG CAGCTACACC CAAGGGGCCC CCGTCCAGCT 
GTGGGACAGT TTGTACCAGA GTGTGATGTA GAGGGAAATT ATGTTCCATT ACAGTGTCAT 
GGCAGCACTG GCCATTGTTG GTGTGTGGAC AAATTAGGTG AAGAGATTGC TGGAACAAGA 
ACACCACCGG GCAGACCAGC CCCAAATTGT GGGGAAACAG GTCCTGAACC CCTGAAAACC 
CCCTGTTTAG AAAGGCGCCA GCAGCTGCTT GGACAGCTAC ACCCAAGAGG CCCCCGTCCA 
GCTGTGGGAC AGTTTGTACC TGAGTGTGAT GCAGAGGGAA ATTATGTTTC ATTACAGTGT 
CATGGCAGCA CTGGCCATTG TTGGTGTGTG GACAAATTAG GTGAAGAGAT TGCTGGAACA 
AGAACACCAC CTGGCAGACC AGGCCCAAAT TGTGGGGAAA CAGAGCCTAC TCAGAGACCT 
CAGACAGTGT GTGAGAGGTG GAAGCAGAGT CTTATTGAGC ATTATGGTGG GAAACCAGGT 
GGAGAACATT ATGTGCCTCA GTGTGATCAA TATGGTGACT TCAGTCCTCT CCAGTGCCAT 
GGAAACAGTG GCTATTGCTG GTGTGTGGAT AAAGAAGGAA GAGAAATTGA AGGATCTAGA 
ACAGAACCTG GAATGACTCC AGCATGTATA CCGACAGTGG CACCTCCAAC CATGCATCCA 
ACTCCGCGTC CTGATGTGAC CCCTCCTGCA ACTGGCACCT TCCTCTTGTA TGCTCAGGGT 
CAGCAGATTG GCTACTTACC ACTCAACGGC ACAAGGCTCC ATAAGGAAAA AGCCAGGACA 
CTGCTGTCAC TTCATGGTTC TATAGTGGTT GGTATTGACT ATGACTGCCA TGAGAAAATG 
GTGTACTGGA CGGATGTTGC AGGTCGTACA ATCAACCGCG CAAGCTTAGA ACCAGGTGCA 
GAACCGGAAA CTATCATTAA CACAGGGTTG ATGAGCACAG AGGGCTTAGC CATTGACTAC 
CTTCGCAGGA CAATGTTTTG GACAGATAGC GGCCTAGACA AGATTGAGAG TGCGCGGTTG 
GACGGCACTG AGAGGAAGGT TCTATTTGAC ACACAGCTTG TGAATCCTCG AGCCATCACT 
GTGGATGCGG TTAGAGGGAA CCTGTACTGG ACAGACTGGA ACAGAGAGGC TCCAAAGATT 
GAATCTTCGT TTGTAGACGG ATCAAACAGG AGGATTCTGG TTAATGACAA CATCGGCCTT 
CCCAACGGTT TAACCTTTGA TCCCTTCTCC AAACAAGTCT GTTGGGCTGA TGCAGGAACC 
AAGAAGCTTG AATGCATTTT GTCAGACGGA ACAGGGCGAC GGGTCATACA GAGTAACTTG 
AATTATCCTT TTAGTGTTGT TGTGTATGCA AATTACTTCT ACCACACAGA CTGGAGGAGG 
GATGGGGTAA TATCCCTTAA CAAGGACACT GGGCACATTG TTGAAGAATA CCTTCCTGAG 
CAGCGATCCC ATCTCTATGG AGTAACAGCT GTGTACCCAT ACTGCCCTTC AGGGAGAAAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001090732.1
CDS	90..4652
Translation	MWDRTLHLLLPLHCLLAFSAAISRHELFPYGELRGDVLLQEGDDETSEVVKLSQPLLFYEAQFSNLYVGTNGIISTQDFPRETQYVDDGFPTDFPVISPFLSDIDTSNGKGKIFYRQDNSEQVLNRAVSEIQRGFPETSFVPTDAFLVTWENVGAYEEMTRLSPSSDRRNTFQAVLAYDHSDAYAIFLYPEDGLQFFGTRPKESYNVHIELPARVGFSRSESGPYYSVTSNEQSVKNLYQTGNTGVPGIWVFHIGSIWELDNVIPAKFFGAPTKEQSLENSHTGFPEHRIFEKNESEDSIDYPESFYEENEDDLEDNTSDATPTHNAHQKVDSDTGNQGYLDIDQASKLTHADVEQEATSSVVLSSSHQNPDWTPYKLSESDTDRDGPLQEPQVQTGEDTDLTRGDTLLTEELPSHKAHNLTPQNSEDLSIYPESEVLVGSHPEQENDFERKQIDADPGLQTDVFSFNPVGKETCERNHGRCSRFAFCADYSAGFCCHCQEDYYGNGVHCLPRGAPHRVNGKVSGNILVGQTPVNFIAADLHAYIVANDGRAYTAISHVPDPASWSLTPLAPIGGLFGWLFALEKPGFQNGFSFTGAKFVHNIDVTFYPGEETVHITQTADGFDSEGYMNVKTAIQGKIPYIPETSTIKLSPYTELYQYSGSVVTSTAYREYTVISESNEEQKLSYRLRQNVTYQECSHSQKQPVSAQKLYVDRVFALYNKEEKVLRYAVTNYIGSLHDSTGQDDLTVNPCYDGTHLCDTRAKCQPGSGLEYSCVCASGYQGDGRDCTDVNECEVGFTRCGQNTVCVNLQGSYRCECASGFTLSGDGHNCILASLINPCEDGSHTCNRDTSRCVPRGDGVFTCECFPGFNKSGEDCVDVDECTEHRCHPDASCTNTLGSFSCRCNSGYEGDGFQCTQILGPEPLKTPCLEKRQQLHPRGPRPAVGQFVPECDVEGNYVPLQCHGSTGHCWCVDKLGEEIAGTRTPPGRPAPNCGETGPEPLKTPCLEKRQQLHPRGPRPAVGQFVPECDVEGNYVPLQCHGSTGHCWCVDKLGEEIAGTRTPPGRPAPNCGETGPEPLKTPCLERRQQLLGQLHPRGPRPAVGQFVPECDAEGNYVSLQCHGSTGHCWCVDKLGEEIAGTRTPPGRPGPNCGETEPTQRPQTVCERWKQSLIEHYGGKPGGEHYVPQCDQYGDFSPLQCHGNSGYCWCVDKEGREIEGSRTEPGMTPACIPTVAPPTMHPTPRPDVTPPATGTFLLYAQGQQIGYLPLNGTRLHKEKARTLLSLHGSIVVGIDYDCHEKMVYWTDVAGRTINRASLEPGAEPETIINTGLMSTEGLAIDYLRRTMFWTDSGLDKIESARLDGTERKVLFDTQLVNPRAITVDAVRGNLYWTDWNREAPKIESSFVDGSNRRILVNDNIGLPNGLTFDPFSKQVCWADAGTKKLECILSDGTGRRVIQSNLNYPFSVVVYANYFYHTDWRRDGVISLNKDTGHIVEEYLPEQRSHLYGVTAVYPYCPSGRK

Target ORF information:

RefSeq Version	NM_001097263.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nidogen 2 (nid2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001097263.1

ORF Insert Sequence:

ATGTGGGACA GAACTCTGCA TCTGCTCCTG CCCTTGCACT GCCTGCTGGC CTTCTCTGCT 
GCTATATCCC GGCATGAACT CTTCCCCTAT GGGGAACTGA GGGGAGATGT GCTCCTGCAG 
GAGGGGGACG ATGAGACTTC TGAGGTGGTG AAACTCTCCC AACCGCTGCT CTTCTATGAA 
GCCCAATTCA GCAATTTATA TGTAGGGACC AATGGAATTA TTTCTACTCA GGACTTCCCC 
AGAGAAACTC AGTATGTGGA TGATGGCTTC CCTACAGACT TCCCTGTCAT TTCTCCATTC 
CTCTCAGATA TAGATACCAG CAATGGCAAA GGAAAGATTT TTTATCGGCA GGACAATTCT 
GAACAAGTTC TCAATCGGGC AGTCAGTGAG ATCCAGAGGG GTTTCCCAGA GACTTCATTT 
GTTCCTACTG ATGCCTTCCT TGTGACTTGG GAAAATGTTG GTGCCTACGA AGAGATGACC 
CGTCTCTCTC CATCGTCCGA TAGGAGAAAC ACGTTCCAGG CGGTACTAGC ATATGATCAC 
TCTGATGCTT ACGCTATATT CCTTTATCCT GAAGATGGAC TCCAGTTTTT TGGTACTCGT 
CCTAAGGAAT CCTACAATGT GCACATTGAG CTGCCTGCCA GAGTGGGCTT TAGCCGATCA 
GAATCGGGAC CATACTACAG TGTAACAAGT AATGAGCAAT CCGTGAAGAA TTTGTATCAA 
ACTGGCAACA CTGGTGTCCC AGGGATTTGG GTCTTTCACA TTGGCAGCAT TTGGGAATTA 
GACAATGTAA TTCCAGCAAA ATTCTTTGGT GCTCCCACAA AGGAACAATC GCTTGAAAAT 
TCACATACAG GTTTCCCAGA ACACAGAATA TTTGAAAAGA ATGAAAGTGA AGACAGTATT 
GATTACCCAG AGTCCTTTTA TGAGGAGAAT GAAGATGATC TGGAAGATAA TACAAGTGAT 
GCCACACCAA CACATAATGC ACACCAAAAA GTTGATTCAG ACACTGGAAA TCAGGGATAC 
TTGGACATAG ATCAGGCTTC CAAACTGACC CATGCAGATG TTGAACAGGA AGCTACATCA 
TCTGTTGTGC TTTCCTCTTC ACATCAGAAT CCTGATTGGA CACCCTACAA ACTCAGTGAA 
TCTGATACTG ACAGAGATGG GCCATTGCAG GAACCTCAAG TTCAGACAGG GGAGGATACA 
GATTTGACAA GGGGAGACAC ATTACTGACT GAAGAGCTGC CATCCCATAA AGCACATAAT 
TTAACACCTC AAAATTCTGA AGATCTCTCA ATATATCCTG AAAGTGAAGT TCTCGTGGGC 
AGTCACCCAG AACAGGAAAA TGATTTTGAA AGAAAGCAGA TTGATGCTGA TCCTGGACTC 
CAAACTGATG TGTTTTCCTT TAACCCTGTG GGCAAGGAGA CATGTGAGAG GAACCACGGG 
AGATGTTCTA GGTTTGCTTT TTGTGCCGAT TACTCTGCTG GCTTCTGCTG TCATTGCCAG 
GAAGATTATT ATGGCAACGG AGTACATTGT TTGCCAAGAG GGGCTCCACA CCGTGTGAAT 
GGTAAAGTGA GCGGAAACAT CCTGGTCGGT CAAACACCTG TCAACTTCAT TGCTGCCGAT 
TTACACGCAT ACATTGTGGC AAATGATGGA AGAGCGTACA CAGCAATCAG CCACGTTCCT 
GATCCTGCAT CCTGGTCTTT AACACCTCTT GCTCCAATTG GGGGACTCTT TGGCTGGCTT 
TTTGCTTTGG AAAAACCAGG CTTTCAAAAT GGTTTTAGCT TTACCGGTGC CAAGTTTGTA 
CACAACATTG ATGTCACATT TTACCCGGGA GAAGAAACTG TTCATATTAC TCAAACAGCA 
GATGGCTTTG ATTCTGAGGG TTATATGAAT GTAAAGACAG CAATTCAGGG CAAAATTCCA 
TATATCCCAG AGACATCGAC CATCAAGCTG TCTCCTTATA CTGAACTGTA TCAATACTCT 
GGCTCAGTTG TAACTTCGAC AGCCTACAGG GAATACACAG TCATCTCAGA ATCAAACGAA 
GAACAAAAAC TGTCTTACCG GTTACGGCAA AATGTCACTT ACCAAGAGTG CAGCCATTCC 
CAGAAACAGC CTGTTAGTGC TCAAAAGCTT TATGTTGATC GTGTCTTTGC CCTTTACAAT 
AAAGAAGAGA AGGTCCTTCG TTATGCAGTT ACAAACTACA TTGGATCTCT GCATGATTCG 
ACTGGACAGG ACGATCTCAC AGTTAACCCG TGCTATGATG GTACCCACTT GTGTGACACA 
AGAGCTAAAT GCCAGCCTGG TAGTGGTCTA GAATACAGTT GTGTTTGTGC GTCTGGATAC 
CAAGGGGATG GAAGAGATTG CACTGATGTA AATGAATGTG AAGTTGGCTT CACCCGGTGC 
GGCCAGAACA CTGTTTGTGT CAACCTCCAG GGGAGTTACC GTTGTGAGTG CGCCAGTGGT 
TTCACTTTAT CTGGAGACGG GCACAACTGC ATCTTGGCTT CACTAATAAA CCCATGTGAA 
GATGGAAGTC ATACATGTAA CAGAGACACA TCACGCTGTG TTCCGCGTGG AGATGGTGTA 
TTCACATGTG AATGTTTCCC TGGATTTAAC AAGAGTGGAG AAGACTGTGT TGATGTTGAT 
GAATGCACTG AGCACAGATG TCATCCAGAT GCCTCATGTA CCAACACCCT GGGCTCTTTC 
TCCTGTCGGT GTAACAGTGG TTATGAAGGA GATGGCTTCC AGTGCACTCA AATCTTAGGT 
CCTGAACCCC TGAAAACCCC CTGTTTAGAA AAGCGCCAGC AGCTACACCC AAGGGGCCCC 
CGTCCAGCTG TGGGACAGTT TGTACCAGAG TGTGATGTAG AGGGAAATTA TGTTCCATTA 
CAGTGTCATG GCAGCACTGG CCATTGTTGG TGTGTGGACA AATTAGGTGA AGAGATTGCT 
GGAACAAGAA CACCACCGGG CAGACCAGCC CCAAATTGTG GGGAAACAGG TCCTGAACCC 
CTGAAAACCC CCTGTTTAGA AAAGCGCCAG CAGCTACACC CAAGGGGCCC CCGTCCAGCT 
GTGGGACAGT TTGTACCAGA GTGTGATGTA GAGGGAAATT ATGTTCCATT ACAGTGTCAT 
GGCAGCACTG GCCATTGTTG GTGTGTGGAC AAATTAGGTG AAGAGATTGC TGGAACAAGA 
ACACCACCGG GCAGACCAGC CCCAAATTGT GGGGAAACAG GTCCTGAACC CCTGAAAACC 
CCCTGTTTAG AAAGGCGCCA GCAGCTGCTT GGACAGCTAC ACCCAAGAGG CCCCCGTCCA 
GCTGTGGGAC AGTTTGTACC TGAGTGTGAT GCAGAGGGAA ATTATGTTTC ATTACAGTGT 
CATGGCAGCA CTGGCCATTG TTGGTGTGTG GACAAATTAG GTGAAGAGAT TGCTGGAACA 
AGAACACCAC CTGGCAGACC AGGCCCAAAT TGTGGGGAAA CAGAGCCTAC TCAGAGACCT 
CAGACAGTGT GTGAGAGGTG GAAGCAGAGT CTTATTGAGC ATTATGGTGG GAAACCAGGT 
GGAGAACATT ATGTGCCTCA GTGTGATCAA TATGGTGACT TCAGTCCTCT CCAGTGCCAT 
GGAAACAGTG GCTATTGCTG GTGTGTGGAT AAAGAAGGAA GAGAAATTGA AGGATCTAGA 
ACAGAACCTG GAATGACTCC AGCATGTATA CCGACAGTGG CACCTCCAAC CATGCATCCA 
ACTCCGCGTC CTGATGTGAC CCCTCCTGCA ACTGGCACCT TCCTCTTGTA TGCTCAGGGT 
CAGCAGATTG GCTACTTACC ACTCAACGGC ACAAGGCTCC ATAAGGAAAA AGCCAGGACA 
CTGCTGTCAC TTCATGGTTC TATAGTGGTT GGTATTGACT ATGACTGCCA TGAGAAAATG 
GTGTACTGGA CGGATGTTGC AGGTCGTACA ATCAACCGCG CAAGCTTAGA ACCAGGTGCA 
GAACCGGAAA CTATCATTAA CACAGGGTTG ATGAGCACAG AGGGCTTAGC CATTGACTAC 
CTTCGCAGGA CAATGTTTTG GACAGATAGC GGCCTAGACA AGATTGAGAG TGCGCGGTTG 
GACGGCACTG AGAGGAAGGT TCTATTTGAC ACACAGCTTG TGAATCCTCG AGCCATCACT 
GTGGATGCGG TTAGAGGGAA CCTGTACTGG ACAGACTGGA ACAGAGAGGC TCCAAAGATT 
GAATCTTCGT TTGTAGACGG ATCAAACAGG AGGATTCTGG TTAATGACAA CATCGGCCTT 
CCCAACGGTT TAACCTTTGA TCCCTTCTCC AAACAAGTCT GTTGGGCTGA TGCAGGAACC 
AAGAAGCTTG AATGCATTTT GTCAGACGGA ACAGGGCGAC GGGTCATACA GAGTAACTTG 
AATTATCCTT TTAGTGTTGT TGTGTATGCA AATTACTTCT ACCACACAGA CTGGAGGAGG 
GATGGGGTAA TATCCCTTAA CAAGGACACT GGGCACATTG TTGAAGAATA CCTTCCTGAG 
CAGCGATCCC ATCTCTATGG AGTAACAGCT GTGTACCCAT ACTGCCCTTC AGGGAGAAAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nid2 ( XM_012967809.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823263.1 Xenopus tropicalis nidogen 2 (nid2), transcript variant X1, mRNA.

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CloneID	OXa29902
Clone ID Related Accession (Same CDS sequence)	XM_012967809.2
Accession Version	XM_012967809.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nidogen-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012967809.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGACA GAACTCTGCA TCTGCTCCTG CCCTTGCACT GCCTGCTGGC CTTCTCTGCT 
GCTATATCCC GGCATGAACT CTTCCCCTAT GGGGAACTGA GGGGAGATGT GCTCCTGCAG 
GAGGGGGACG ATGAGACTTC TGAGGTGGTG AAACTCTCCC AACCGCTGCT CTTCTATGAA 
GCCCAATTCA GCAATTTATA TGTAGGGACC AATGGAATTA TTTCCACTCA GGACTTCCCC 
AGAGAAACTC AGTATGTGGA TGATGGCTTC CCTACAGACT TCCCTGTCAT TGCTCCATTC 
CTCTCAGATA TAGATACCAG CAATGGCAAA GGAAAGATTT TTTATCGGCA GGACAATTCT 
GAACAAGTTC TCAATCGGGC AGTCAGTGAG ATCCAGAGGG GTTTCCCAGA GACTTCATTT 
GTTCCTACTG ATGCCTTCCT TGTGACTTGG GAAAATGTTG GTGCCTACGA AGAGATGACC 
CGTCACTCTC CATCGTCTGA TAGGAGAAAC ACGTTCCAGG CGGTACTAGC ATATGATCAC 
TCTGATGCTT ACGCTATATT CCTTTATCCT GAAGATGGAC TCCAGTTTTT TGGTACTCGT 
CCTAAGGAAT CCTACAATGT GCACATTGAG CTGCCTGCCA GAGTGGGCTT TAGCCGATCA 
GAATCGGGAC CATACTACAG TGTAACAAGT AATGAGCAAT CCGTGAAGAA TTTGTATCAA 
ACTGGAAACA CTGGTGTCCC AGGGATTTGG GTCTTTCACA TTGGCAGCAT TTGGGAATTA 
GACAATGTAA TTCCAGCAAA ATTCTTTGGT GCTCCCACAA AGGAACAATC GCTTGAAAAT 
TCACATACAG GTTTCCCAGA ACACAGAATA TTTGAAAAGA ATGAAAGTGA AGACAGTATT 
GATTACCCAG AGTCCTTTTA TGAGGAGAAT GAAGATGATC TGGAAGATAA TACAAGTGAT 
GCCACACCAA CACATAATGC ACACCAAAAA GTTGATTCAG ACACTGGAAA TCAGGGATAC 
TTGGACATAG ATCAGGCTTC CAAACTGACC CATGCAGATG TTGAACAGGA AGCTACATCA 
TCTGTTGTGC TTTCCTCTTC ACATCAGAAT CCTGATTGGA CACCCTACAA ACTCAGTGAA 
TCTGATACTG ACAGAGATGG GCCATTGCAG GAACCTCAAG TTCAGACAGG GGAGGATACA 
GATTTGACAA GGGGAGACAC ATTACTGACT GAAGAGCTGC CATCCCATAA AGCACATAAT 
TTAACACCTC AAAATTCTGA AGATCTCTCA ATATATCCTG AAAGTGAAGT TCTCGTGGGC 
AGTCACCCAG AACAGGAAAA TGATTTTGAA AGAAAGCAGA TTGATGCTGA TCCTGGACTC 
CAAACTGATG TGTTTTCCTT TAACCCTGTG GGCAAGGAGA CATGTGAGAG GAACCACGGG 
AGATGTTCTA GGTTTGCTTT TTGTGCCGAT TACTCTGCTG GCTTCTGCTG TCATTGCCAG 
GAAGATTATT ATGGCAACGG AGTACATTGT TTGCCAAGAG GGGCTCCACA CCGTGTGAAT 
GGTAAAGTGA GCGGAAACAT CCTGGTCGGT CAAACACCTG TCAACTTCAT TGCTGCCGAT 
TTACACGCAT ACATTGTGGC AAATGATGGA AGAGCGTACA CAGCAATCAG CCACGTTCCT 
GATCCTGCAT CCTGGTCTTT AACACCTCTT GCTCCAATTG GGGGACTCTT TGGCTGGCTT 
TTTGCTTTGG AAAAACCAGG CTTTCAAAAT GGTTTTAGCT TTACCGGTGC CAAGTTTGTA 
CACAACATTG ATGTCACATT TTACCCGGGA GAAGAAACTG TTCATATTAC TCAAACAGCA 
GATGGCTTTG ATTCTGAGGG TTATATGAAT GTAAAGACAG CAATTCAGGG CAAAATTCCA 
TATATCCCAG AGACATCGAC CATCAAGCTG TCTCCTTATA CTGAACTGTA TCAATACTCT 
GGCTCAGTTG TAACTTCGAC AGCCTACAGG GAATACACAG TCATCTCAGA ATCAAACGAA 
GAACAAAAAC TGTCTTACCG GTTACGGCAA AATGTCACTT ACCAAGAGTG CAGCCATTCC 
CAGAAACAGC CTGTTAGTGC TCAAAAGCTT TATGTTGATC GTGTCTTTGC CCTTTACAAT 
AAAGAAGAGA AGGTCCTTCG TTATGCAGTT ACAAACTACA TTGGATCTCT GCATGATTCG 
ACTGGACAGG ACGATCTCAC AGTTAACCCG TGCTATGATG GTACCCACTT GTGTGACACA 
AGAGCTAAAT GCCAGCCTGG TAGTGGTCTA GAATACAGTT GTGTTTGTGC GTCTGGATAC 
CAAGGGGATG GAAGAGATTG CACTGATGTA AATGAATGTG AAGTTGGCTT CACCCGGTGC 
GGCCAGAACA CTGTTTGTGT CAACCTCCAG GGGAGTTACC GTTGTGAGTG CGCCAGTGGT 
TTCACTTTAT CTGGAGACGG GCACAACTGC ATCTTGGCTT CACTAATAAA CCCATGTGAA 
GATGGAAGTC ATACATGTAA CAGAGACACA TCACGCTGTG TTCCGCGTGG AGATGGTGTA 
TTCACATGTG AATGTTTCCC TGGATTTAAC AAGAGTGGAG AAGACTGTGT TGATGTTGAT 
GAATGCACTG AGCACAGATG TCATCCAGAT GCCTCATGTA CCAACACCCT GGGCTCTTTC 
TCCTGTCGGT GTAACAGTGG TTATGAAGGA GATGGCTTCC AGTGCACTCA AATCTTAGGT 
CCTGAACCCC TGAAAACCCC CTGTTTAGAA AAGCGCCAGC AGCTACACCC AAGGGGCCCC 
CGTCCAGCTG TGGGACAGTT TGTACCAGAG TGTGATGTAG AGGGAAATTA TGTTCCATTA 
CAGTGTCATG GCAGCACTGG CCATTGTTGG TGTGTGGACA AATTAGGTGA AGAGATTACT 
GGAACAAGAA CACCACCGGG CAGACCAGCC CCAAATTGTG GGGAAAGAGG TCCTGAACCC 
CTGAAAACCC CCTGTTTAGA AAAGCGCCAG CAGCTACACC CAAGGGGCCC CCGTCCAGCT 
GTGGGACAGT TTGTACCAGA GTGTGATGTA GAGGGAAATT ATGTTCCATT ACAGTGTCAT 
GGCAGCACTG GCCATTGTTG GTGTGTGGAC AAATTAGGTG AAGAGATTAC TGGAACAAGA 
ACACCACCGG GCAGACCAGC CCCAAATTGT GGGGAAACAG GTCCTGAACC CCTGAAAACC 
CCCTGTTTAG AAAGGCGCCA GCAGCTGCTT GGACAGCTGC ACCCAAGAGG CCCCCGTCCA 
GCTGTGGGAC AGTTTGTACC TGAGTGTGAT GCAGAGGGAA ATTATGTTTC ATTACAGTGT 
CATGGCAGCA CTGGCCATTG TTGGTGTGTG GACAAATTAG GTGAAGAGAT TGCTGGAACA 
AGAACACCAC CTGGCAGACC AGGCCCAAAT TGTGGGGAAA CAGCAGAGCC TACTCGGAGA 
CCTCAGACAG TGTGTGAGAG GTGGAAGCAG AGTCTTATTG AGCATTATGG TGGGAAACCA 
GGTGGAGAAC ATTATGTGCC TCAGTGTGAT CAATATGGTG ACTTCAGTCC TCTCCAGTGC 
CATGGAAACA GTGGCTATTG CTGGTGTGTG GATAAAGAAG GAAGAGAAAT TGAAGGATCT 
AGAACAGAAC CTGGAATGAC TCCAGCATGT ATACCGACAG TGGCACCTCC AACCATGCAT 
CCAACTCCGC GTCCTGATGT GACCCCTCCT GCAACTGGCA CCTTCCTCTT GTATGCTCAG 
GGTCAGCAGA TTGGCTACTT ACCACTCAAC GGCACAAGGC TCCATAAGGA AAAAGCCAGG 
ACACTGCTGT CACTTCATGG TTCTATAGTG GTTGGTATTG ACTATGACTG CCATGAGAAA 
ATGGTGTACT GGACGGATGT TGCAGGTCGT ACAATCAACC GCGCAAGCTT AGAACCAGGT 
GCAGAACCGG AAACTATCAT TAACACAGGG TTGATGAGCA CAGAGGGCTT AGCCATTGAC 
TACCTTCGCA GGACAATGTT TTGGACAGAT AGCGGCCTAG ACAAGATTGA GAGTGCGCGG 
TTGGACGGCA CTGAGAGGAA GGTTCTATTT GACACACAGC TTGTGAATCC TCGAGCCATC 
ACTGTGGATG CGGTTAGAGG GAACCTGTAC TGGACAGACT GGAACAGAGA GGCTCCAAAG 
ATTGAATCTT CGTTTGTAGA CGGATCAAAC AGGAGGATTC TGGTTAATGA CAACATCGGC 
CTTCCCAACG GTTTAACCTT TGATCCCTTC TCCAAACAAG TCTGTTGGGC TGATGCAGGA 
ACCAAGAAGC TTGAATGCAT TTTGTCAGAC GGAACAGGGC GACGGGTCAT ACAGAGTAAC 
TTGAATTATC CTTTTAGTGT TGTTGTGTAT GCAAATTACT TCTACCACAC AGACTGGAGG 
AGGGATGGGG TAATATCCCT TAACAAGGAC ACTGGGCACA TTGTTGAAGA ATACCTTCCT 
GAGCAGCGAT CCCATCTCTA TGGAGTAACA GCTGTGTACC CATACTGCCC TTCAGGGAGA 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012823263.1
CDS	100..4665
Translation	MWDRTLHLLLPLHCLLAFSAAISRHELFPYGELRGDVLLQEGDDETSEVVKLSQPLLFYEAQFSNLYVGTNGIISTQDFPRETQYVDDGFPTDFPVIAPFLSDIDTSNGKGKIFYRQDNSEQVLNRAVSEIQRGFPETSFVPTDAFLVTWENVGAYEEMTRHSPSSDRRNTFQAVLAYDHSDAYAIFLYPEDGLQFFGTRPKESYNVHIELPARVGFSRSESGPYYSVTSNEQSVKNLYQTGNTGVPGIWVFHIGSIWELDNVIPAKFFGAPTKEQSLENSHTGFPEHRIFEKNESEDSIDYPESFYEENEDDLEDNTSDATPTHNAHQKVDSDTGNQGYLDIDQASKLTHADVEQEATSSVVLSSSHQNPDWTPYKLSESDTDRDGPLQEPQVQTGEDTDLTRGDTLLTEELPSHKAHNLTPQNSEDLSIYPESEVLVGSHPEQENDFERKQIDADPGLQTDVFSFNPVGKETCERNHGRCSRFAFCADYSAGFCCHCQEDYYGNGVHCLPRGAPHRVNGKVSGNILVGQTPVNFIAADLHAYIVANDGRAYTAISHVPDPASWSLTPLAPIGGLFGWLFALEKPGFQNGFSFTGAKFVHNIDVTFYPGEETVHITQTADGFDSEGYMNVKTAIQGKIPYIPETSTIKLSPYTELYQYSGSVVTSTAYREYTVISESNEEQKLSYRLRQNVTYQECSHSQKQPVSAQKLYVDRVFALYNKEEKVLRYAVTNYIGSLHDSTGQDDLTVNPCYDGTHLCDTRAKCQPGSGLEYSCVCASGYQGDGRDCTDVNECEVGFTRCGQNTVCVNLQGSYRCECASGFTLSGDGHNCILASLINPCEDGSHTCNRDTSRCVPRGDGVFTCECFPGFNKSGEDCVDVDECTEHRCHPDASCTNTLGSFSCRCNSGYEGDGFQCTQILGPEPLKTPCLEKRQQLHPRGPRPAVGQFVPECDVEGNYVPLQCHGSTGHCWCVDKLGEEITGTRTPPGRPAPNCGERGPEPLKTPCLEKRQQLHPRGPRPAVGQFVPECDVEGNYVPLQCHGSTGHCWCVDKLGEEITGTRTPPGRPAPNCGETGPEPLKTPCLERRQQLLGQLHPRGPRPAVGQFVPECDAEGNYVSLQCHGSTGHCWCVDKLGEEIAGTRTPPGRPGPNCGETAEPTRRPQTVCERWKQSLIEHYGGKPGGEHYVPQCDQYGDFSPLQCHGNSGYCWCVDKEGREIEGSRTEPGMTPACIPTVAPPTMHPTPRPDVTPPATGTFLLYAQGQQIGYLPLNGTRLHKEKARTLLSLHGSIVVGIDYDCHEKMVYWTDVAGRTINRASLEPGAEPETIINTGLMSTEGLAIDYLRRTMFWTDSGLDKIESARLDGTERKVLFDTQLVNPRAITVDAVRGNLYWTDWNREAPKIESSFVDGSNRRILVNDNIGLPNGLTFDPFSKQVCWADAGTKKLECILSDGTGRRVIQSNLNYPFSVVVYANYFYHTDWRRDGVISLNKDTGHIVEEYLPEQRSHLYGVTAVYPYCPSGRK

Target ORF information:

RefSeq Version	XM_012967809.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nidogen 2 (nid2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967809.2

ORF Insert Sequence:

ATGTGGGACA GAACTCTGCA TCTGCTCCTG CCCTTGCACT GCCTGCTGGC CTTCTCTGCT 
GCTATATCCC GGCATGAACT CTTCCCCTAT GGGGAACTGA GGGGAGATGT GCTCCTGCAG 
GAGGGGGACG ATGAGACTTC TGAGGTGGTG AAACTCTCCC AACCGCTGCT CTTCTATGAA 
GCCCAATTCA GCAATTTATA TGTAGGGACC AATGGAATTA TTTCCACTCA GGACTTCCCC 
AGAGAAACTC AGTATGTGGA TGATGGCTTC CCTACAGACT TCCCTGTCAT TGCTCCATTC 
CTCTCAGATA TAGATACCAG CAATGGCAAA GGAAAGATTT TTTATCGGCA GGACAATTCT 
GAACAAGTTC TCAATCGGGC AGTCAGTGAG ATCCAGAGGG GTTTCCCAGA GACTTCATTT 
GTTCCTACTG ATGCCTTCCT TGTGACTTGG GAAAATGTTG GTGCCTACGA AGAGATGACC 
CGTCACTCTC CATCGTCTGA TAGGAGAAAC ACGTTCCAGG CGGTACTAGC ATATGATCAC 
TCTGATGCTT ACGCTATATT CCTTTATCCT GAAGATGGAC TCCAGTTTTT TGGTACTCGT 
CCTAAGGAAT CCTACAATGT GCACATTGAG CTGCCTGCCA GAGTGGGCTT TAGCCGATCA 
GAATCGGGAC CATACTACAG TGTAACAAGT AATGAGCAAT CCGTGAAGAA TTTGTATCAA 
ACTGGAAACA CTGGTGTCCC AGGGATTTGG GTCTTTCACA TTGGCAGCAT TTGGGAATTA 
GACAATGTAA TTCCAGCAAA ATTCTTTGGT GCTCCCACAA AGGAACAATC GCTTGAAAAT 
TCACATACAG GTTTCCCAGA ACACAGAATA TTTGAAAAGA ATGAAAGTGA AGACAGTATT 
GATTACCCAG AGTCCTTTTA TGAGGAGAAT GAAGATGATC TGGAAGATAA TACAAGTGAT 
GCCACACCAA CACATAATGC ACACCAAAAA GTTGATTCAG ACACTGGAAA TCAGGGATAC 
TTGGACATAG ATCAGGCTTC CAAACTGACC CATGCAGATG TTGAACAGGA AGCTACATCA 
TCTGTTGTGC TTTCCTCTTC ACATCAGAAT CCTGATTGGA CACCCTACAA ACTCAGTGAA 
TCTGATACTG ACAGAGATGG GCCATTGCAG GAACCTCAAG TTCAGACAGG GGAGGATACA 
GATTTGACAA GGGGAGACAC ATTACTGACT GAAGAGCTGC CATCCCATAA AGCACATAAT 
TTAACACCTC AAAATTCTGA AGATCTCTCA ATATATCCTG AAAGTGAAGT TCTCGTGGGC 
AGTCACCCAG AACAGGAAAA TGATTTTGAA AGAAAGCAGA TTGATGCTGA TCCTGGACTC 
CAAACTGATG TGTTTTCCTT TAACCCTGTG GGCAAGGAGA CATGTGAGAG GAACCACGGG 
AGATGTTCTA GGTTTGCTTT TTGTGCCGAT TACTCTGCTG GCTTCTGCTG TCATTGCCAG 
GAAGATTATT ATGGCAACGG AGTACATTGT TTGCCAAGAG GGGCTCCACA CCGTGTGAAT 
GGTAAAGTGA GCGGAAACAT CCTGGTCGGT CAAACACCTG TCAACTTCAT TGCTGCCGAT 
TTACACGCAT ACATTGTGGC AAATGATGGA AGAGCGTACA CAGCAATCAG CCACGTTCCT 
GATCCTGCAT CCTGGTCTTT AACACCTCTT GCTCCAATTG GGGGACTCTT TGGCTGGCTT 
TTTGCTTTGG AAAAACCAGG CTTTCAAAAT GGTTTTAGCT TTACCGGTGC CAAGTTTGTA 
CACAACATTG ATGTCACATT TTACCCGGGA GAAGAAACTG TTCATATTAC TCAAACAGCA 
GATGGCTTTG ATTCTGAGGG TTATATGAAT GTAAAGACAG CAATTCAGGG CAAAATTCCA 
TATATCCCAG AGACATCGAC CATCAAGCTG TCTCCTTATA CTGAACTGTA TCAATACTCT 
GGCTCAGTTG TAACTTCGAC AGCCTACAGG GAATACACAG TCATCTCAGA ATCAAACGAA 
GAACAAAAAC TGTCTTACCG GTTACGGCAA AATGTCACTT ACCAAGAGTG CAGCCATTCC 
CAGAAACAGC CTGTTAGTGC TCAAAAGCTT TATGTTGATC GTGTCTTTGC CCTTTACAAT 
AAAGAAGAGA AGGTCCTTCG TTATGCAGTT ACAAACTACA TTGGATCTCT GCATGATTCG 
ACTGGACAGG ACGATCTCAC AGTTAACCCG TGCTATGATG GTACCCACTT GTGTGACACA 
AGAGCTAAAT GCCAGCCTGG TAGTGGTCTA GAATACAGTT GTGTTTGTGC GTCTGGATAC 
CAAGGGGATG GAAGAGATTG CACTGATGTA AATGAATGTG AAGTTGGCTT CACCCGGTGC 
GGCCAGAACA CTGTTTGTGT CAACCTCCAG GGGAGTTACC GTTGTGAGTG CGCCAGTGGT 
TTCACTTTAT CTGGAGACGG GCACAACTGC ATCTTGGCTT CACTAATAAA CCCATGTGAA 
GATGGAAGTC ATACATGTAA CAGAGACACA TCACGCTGTG TTCCGCGTGG AGATGGTGTA 
TTCACATGTG AATGTTTCCC TGGATTTAAC AAGAGTGGAG AAGACTGTGT TGATGTTGAT 
GAATGCACTG AGCACAGATG TCATCCAGAT GCCTCATGTA CCAACACCCT GGGCTCTTTC 
TCCTGTCGGT GTAACAGTGG TTATGAAGGA GATGGCTTCC AGTGCACTCA AATCTTAGGT 
CCTGAACCCC TGAAAACCCC CTGTTTAGAA AAGCGCCAGC AGCTACACCC AAGGGGCCCC 
CGTCCAGCTG TGGGACAGTT TGTACCAGAG TGTGATGTAG AGGGAAATTA TGTTCCATTA 
CAGTGTCATG GCAGCACTGG CCATTGTTGG TGTGTGGACA AATTAGGTGA AGAGATTACT 
GGAACAAGAA CACCACCGGG CAGACCAGCC CCAAATTGTG GGGAAAGAGG TCCTGAACCC 
CTGAAAACCC CCTGTTTAGA AAAGCGCCAG CAGCTACACC CAAGGGGCCC CCGTCCAGCT 
GTGGGACAGT TTGTACCAGA GTGTGATGTA GAGGGAAATT ATGTTCCATT ACAGTGTCAT 
GGCAGCACTG GCCATTGTTG GTGTGTGGAC AAATTAGGTG AAGAGATTAC TGGAACAAGA 
ACACCACCGG GCAGACCAGC CCCAAATTGT GGGGAAACAG GTCCTGAACC CCTGAAAACC 
CCCTGTTTAG AAAGGCGCCA GCAGCTGCTT GGACAGCTGC ACCCAAGAGG CCCCCGTCCA 
GCTGTGGGAC AGTTTGTACC TGAGTGTGAT GCAGAGGGAA ATTATGTTTC ATTACAGTGT 
CATGGCAGCA CTGGCCATTG TTGGTGTGTG GACAAATTAG GTGAAGAGAT TGCTGGAACA 
AGAACACCAC CTGGCAGACC AGGCCCAAAT TGTGGGGAAA CAGCAGAGCC TACTCGGAGA 
CCTCAGACAG TGTGTGAGAG GTGGAAGCAG AGTCTTATTG AGCATTATGG TGGGAAACCA 
GGTGGAGAAC ATTATGTGCC TCAGTGTGAT CAATATGGTG ACTTCAGTCC TCTCCAGTGC 
CATGGAAACA GTGGCTATTG CTGGTGTGTG GATAAAGAAG GAAGAGAAAT TGAAGGATCT 
AGAACAGAAC CTGGAATGAC TCCAGCATGT ATACCGACAG TGGCACCTCC AACCATGCAT 
CCAACTCCGC GTCCTGATGT GACCCCTCCT GCAACTGGCA CCTTCCTCTT GTATGCTCAG 
GGTCAGCAGA TTGGCTACTT ACCACTCAAC GGCACAAGGC TCCATAAGGA AAAAGCCAGG 
ACACTGCTGT CACTTCATGG TTCTATAGTG GTTGGTATTG ACTATGACTG CCATGAGAAA 
ATGGTGTACT GGACGGATGT TGCAGGTCGT ACAATCAACC GCGCAAGCTT AGAACCAGGT 
GCAGAACCGG AAACTATCAT TAACACAGGG TTGATGAGCA CAGAGGGCTT AGCCATTGAC 
TACCTTCGCA GGACAATGTT TTGGACAGAT AGCGGCCTAG ACAAGATTGA GAGTGCGCGG 
TTGGACGGCA CTGAGAGGAA GGTTCTATTT GACACACAGC TTGTGAATCC TCGAGCCATC 
ACTGTGGATG CGGTTAGAGG GAACCTGTAC TGGACAGACT GGAACAGAGA GGCTCCAAAG 
ATTGAATCTT CGTTTGTAGA CGGATCAAAC AGGAGGATTC TGGTTAATGA CAACATCGGC 
CTTCCCAACG GTTTAACCTT TGATCCCTTC TCCAAACAAG TCTGTTGGGC TGATGCAGGA 
ACCAAGAAGC TTGAATGCAT TTTGTCAGAC GGAACAGGGC GACGGGTCAT ACAGAGTAAC 
TTGAATTATC CTTTTAGTGT TGTTGTGTAT GCAAATTACT TCTACCACAC AGACTGGAGG 
AGGGATGGGG TAATATCCCT TAACAAGGAC ACTGGGCACA TTGTTGAAGA ATACCTTCCT 
GAGCAGCGAT CCCATCTCTA TGGAGTAACA GCTGTGTACC CATACTGCCC TTCAGGGAGA 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nid2 ( XM_012967810.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823264.1 Xenopus tropicalis nidogen 2 (nid2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa29903
Clone ID Related Accession (Same CDS sequence)	XM_012967810.2
Accession Version	XM_012967810.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4332bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nidogen-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012967810.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGACA GAACTCTGCA TCTGCTCCTG CCCTTGCACT GCCTGCTGGC CTTCTCTGCT 
GCTATATCCC GGCATGAACT CTTCCCCTAT GGGGAACTGA GGGGAGATGT GCTCCTGCAG 
GAGGGGGACG ATGAGACTTC TGAGGTGGTG AAACTCTCCC AACCGCTGCT CTTCTATGAA 
GCCCAATTCA GCAATTTATA TGTAGGGACC AATGGAATTA TTTCCACTCA GGACTTCCCC 
AGAGAAACTC AGTATGTGGA TGATGGCTTC CCTACAGACT TCCCTGTCAT TGCTCCATTC 
CTCTCAGATA TAGATACCAG CAATGGCAAA GGAAAGATTT TTTATCGGCA GGACAATTCT 
GAACAAGTTC TCAATCGGGC AGTCAGTGAG ATCCAGAGGG GTTTCCCAGA GACTTCATTT 
GTTCCTACTG ATGCCTTCCT TGTGACTTGG GAAAATGTTG GTGCCTACGA AGAGATGACC 
CGTCACTCTC CATCGTCTGA TAGGAGAAAC ACGTTCCAGG CGGTACTAGC ATATGATCAC 
TCTGATGCTT ACGCTATATT CCTTTATCCT GAAGATGGAC TCCAGTTTTT TGGTACTCGT 
CCTAAGGAAT CCTACAATGT GCACATTGAG CTGCCTGCCA GAGTGGGCTT TAGCCGATCA 
GAATCGGGAC CATACTACAG TGTAACAAGT AATGAGCAAT CCGTGAAGAA TTTGTATCAA 
ACTGGAAACA CTGGTGTCCC AGGGATTTGG GTCTTTCACA TTGGCAGCAT TTGGGAATTA 
GACAATGTAA TTCCAGCAAA ATTCTTTGGT GCTCCCACAA AGGAACAATC GCTTGAAAAT 
TCACATACAG GTTTCCCAGA ACACAGAATA TTTGAAAAGA ATGAAAGTGA AGACAGTATT 
GATTACCCAG AGTCCTTTTA TGAGGAGAAT GAAGATGATC TGGAAGATAA TACAAGTGAT 
GCCACACCAA CACATAATGC ACACCAAAAA GTTGATTCAG ACACTGGAAA TCAGGGATAC 
TTGGACATAG ATCAGGCTTC CAAACTGACC CATGCAGATG TTGAACAGGA AGCTACATCA 
TCTGTTGTGC TTTCCTCTTC ACATCAGAAT CCTGATTGGA CACCCTACAA ACTCAGTGAA 
TCTGATACTG ACAGAGATGG GCCATTGCAG GAACCTCAAG TTCAGACAGG GGAGGATACA 
GATTTGACAA GGGGAGACAC ATTACTGACT GAAGAGCTGC CATCCCATAA AGCACATAAT 
TTAACACCTC AAAATTCTGA AGATCTCTCA ATATATCCTG AAAGTGAAGT TCTCGTGGGC 
AGTCACCCAG AACAGGAAAA TGATTTTGAA AGAAAGCAGA TTGATGCTGA TCCTGGACTC 
CAAACTGATG TGTTTTCCTT TAACCCTGTG GGCAAGGAGA CATGTGAGAG GAACCACGGG 
AGATGTTCTA GGTTTGCTTT TTGTGCCGAT TACTCTGCTG GCTTCTGCTG TCATTGCCAG 
GAAGATTATT ATGGCAACGG AGTACATTGT TTGCCAAGAG GGGCTCCACA CCGTGTGAAT 
GGTAAAGTGA GCGGAAACAT CCTGGTCGGT CAAACACCTG TCAACTTCAT TGCTGCCGAT 
TTACACGCAT ACATTGTGGC AAATGATGGA AGAGCGTACA CAGCAATCAG CCACGTTCCT 
GATCCTGCAT CCTGGTCTTT AACACCTCTT GCTCCAATTG GGGGACTCTT TGGCTGGCTT 
TTTGCTTTGG AAAAACCAGG CTTTCAAAAT GGTTTTAGCT TTACCGGTGC CAAGTTTGTA 
CACAACATTG ATGTCACATT TTACCCGGGA GAAGAAACTG TTCATATTAC TCAAACAGCA 
GATGGCTTTG ATTCTGAGGG TTATATGAAT GTAAAGACAG CAATTCAGGG CAAAATTCCA 
TATATCCCAG AGACATCGAC CATCAAGCTG TCTCCTTATA CTGAACTGTA TCAATACTCT 
GGCTCAGTTG TAACTTCGAC AGCCTACAGG GAATACACAG TCATCTCAGA ATCAAACGAA 
GAACAAAAAC TGTCTTACCG GTTACGGCAA AATGTCACTT ACCAAGAGTG CAGCCATTCC 
CAGAAACAGC CTGTTAGTGC TCAAAAGCTT TATGTTGATC GTGTCTTTGC CCTTTACAAT 
AAAGAAGAGA AGGTCCTTCG TTATGCAGTT ACAAACTACA TTGGATCTCT GCATGATTCG 
ACTGGACAGG ACGATCTCAC AGTTAACCCG TGCTATGATG GTACCCACTT GTGTGACACA 
AGAGCTAAAT GCCAGCCTGG TAGTGGTCTA GAATACAGTT GTGTTTGTGC GTCTGGATAC 
CAAGGGGATG GAAGAGATTG CACTGATGTA AATGAATGTG AAGTTGGCTT CACCCGGTGC 
GGCCAGAACA CTGTTTGTGT CAACCTCCAG GGGAGTTACC GTTGTGAGTG CGCCAGTGGT 
TTCACTTTAT CTGGAGACGG GCACAACTGC ATCTTGGCTT CACTAATAAA CCCATGTGAA 
GATGGAAGTC ATACATGTAA CAGAGACACA TCACGCTGTG TTCCGCGTGG AGATGGTGTA 
TTCACATGTG AATGTTTCCC TGGATTTAAC AAGAGTGGAG AAGACTGTGT TGATGTTGAT 
GAATGCACTG AGCACAGATG TCATCCAGAT GCCTCATGTA CCAACACCCT GGGCTCTTTC 
TCCTGTCGGT GTAACAGTGG TTATGAAGGA GATGGCTTCC AGTGCACTCA AATCTTAGGT 
CCTGAACCCC TGAAAACCCC CTGTTTAGAA AAGCGCCAGC AGCTACACCC AAGGGGCCCC 
CGTCCAGCTG TGGGACAGTT TGTACCAGAG TGTGATGTAG AGGGAAATTA TGTTCCATTA 
CAGTGTCATG GCAGCACTGG CCATTGTTGG TGTGTGGACA AATTAGGTGA AGAGATTACT 
GGAACAAGAA CACCACCGGG CAGACCAGCC CCAAATTGTG GGGAAAGAGG TCCTGAACCC 
CTGAAAACCC CCTGTTTAGA AAGGCGCCAG CAGCTGCTTG GACAGCTGCA CCCAAGAGGC 
CCCCGTCCAG CTGTGGGACA GTTTGTACCT GAGTGTGATG CAGAGGGAAA TTATGTTTCA 
TTACAGTGTC ATGGCAGCAC TGGCCATTGT TGGTGTGTGG ACAAATTAGG TGAAGAGATT 
GCTGGAACAA GAACACCACC TGGCAGACCA GGCCCAAATT GTGGGGAAAC AGAGCCTACT 
CGGAGACCTC AGACAGTGTG TGAGAGGTGG AAGCAGAGTC TTATTGAGCA TTATGGTGGG 
AAACCAGGTG GAGAACATTA TGTGCCTCAG TGTGATCAAT ATGGTGACTT CAGTCCTCTC 
CAGTGCCATG GAAACAGTGG CTATTGCTGG TGTGTGGATA AAGAAGGAAG AGAAATTGAA 
GGATCTAGAA CAGAACCTGG AATGACTCCA GCATGTATAC CGACAGTGGC ACCTCCAACC 
ATGCATCCAA CTCCGCGTCC TGATGTGACC CCTCCTGCAA CTGGCACCTT CCTCTTGTAT 
GCTCAGGGTC AGCAGATTGG CTACTTACCA CTCAACGGCA CAAGGCTCCA TAAGGAAAAA 
GCCAGGACAC TGCTGTCACT TCATGGTTCT ATAGTGGTTG GTATTGACTA TGACTGCCAT 
GAGAAAATGG TGTACTGGAC GGATGTTGCA GGTCGTACAA TCAACCGCGC AAGCTTAGAA 
CCAGGTGCAG AACCGGAAAC TATCATTAAC ACAGGGTTGA TGAGCACAGA GGGCTTAGCC 
ATTGACTACC TTCGCAGGAC AATGTTTTGG ACAGATAGCG GCCTAGACAA GATTGAGAGT 
GCGCGGTTGG ACGGCACTGA GAGGAAGGTT CTATTTGACA CACAGCTTGT GAATCCTCGA 
GCCATCACTG TGGATGCGGT TAGAGGGAAC CTGTACTGGA CAGACTGGAA CAGAGAGGCT 
CCAAAGATTG AATCTTCGTT TGTAGACGGA TCAAACAGGA GGATTCTGGT TAATGACAAC 
ATCGGCCTTC CCAACGGTTT AACCTTTGAT CCCTTCTCCA AACAAGTCTG TTGGGCTGAT 
GCAGGAACCA AGAAGCTTGA ATGCATTTTG TCAGACGGAA CAGGGCGACG GGTCATACAG 
AGTAACTTGA ATTATCCTTT TAGTGTTGTT GTGTATGCAA ATTACTTCTA CCACACAGAC 
TGGAGGAGGG ATGGGGTAAT ATCCCTTAAC AAGGACACTG GGCACATTGT TGAAGAATAC 
CTTCCTGAGC AGCGATCCCA TCTCTATGGA GTAACAGCTG TGTACCCATA CTGCCCTTCA 
GGGAGAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012823264.1
CDS	100..4431
Translation	MWDRTLHLLLPLHCLLAFSAAISRHELFPYGELRGDVLLQEGDDETSEVVKLSQPLLFYEAQFSNLYVGTNGIISTQDFPRETQYVDDGFPTDFPVIAPFLSDIDTSNGKGKIFYRQDNSEQVLNRAVSEIQRGFPETSFVPTDAFLVTWENVGAYEEMTRHSPSSDRRNTFQAVLAYDHSDAYAIFLYPEDGLQFFGTRPKESYNVHIELPARVGFSRSESGPYYSVTSNEQSVKNLYQTGNTGVPGIWVFHIGSIWELDNVIPAKFFGAPTKEQSLENSHTGFPEHRIFEKNESEDSIDYPESFYEENEDDLEDNTSDATPTHNAHQKVDSDTGNQGYLDIDQASKLTHADVEQEATSSVVLSSSHQNPDWTPYKLSESDTDRDGPLQEPQVQTGEDTDLTRGDTLLTEELPSHKAHNLTPQNSEDLSIYPESEVLVGSHPEQENDFERKQIDADPGLQTDVFSFNPVGKETCERNHGRCSRFAFCADYSAGFCCHCQEDYYGNGVHCLPRGAPHRVNGKVSGNILVGQTPVNFIAADLHAYIVANDGRAYTAISHVPDPASWSLTPLAPIGGLFGWLFALEKPGFQNGFSFTGAKFVHNIDVTFYPGEETVHITQTADGFDSEGYMNVKTAIQGKIPYIPETSTIKLSPYTELYQYSGSVVTSTAYREYTVISESNEEQKLSYRLRQNVTYQECSHSQKQPVSAQKLYVDRVFALYNKEEKVLRYAVTNYIGSLHDSTGQDDLTVNPCYDGTHLCDTRAKCQPGSGLEYSCVCASGYQGDGRDCTDVNECEVGFTRCGQNTVCVNLQGSYRCECASGFTLSGDGHNCILASLINPCEDGSHTCNRDTSRCVPRGDGVFTCECFPGFNKSGEDCVDVDECTEHRCHPDASCTNTLGSFSCRCNSGYEGDGFQCTQILGPEPLKTPCLEKRQQLHPRGPRPAVGQFVPECDVEGNYVPLQCHGSTGHCWCVDKLGEEITGTRTPPGRPAPNCGERGPEPLKTPCLERRQQLLGQLHPRGPRPAVGQFVPECDAEGNYVSLQCHGSTGHCWCVDKLGEEIAGTRTPPGRPGPNCGETEPTRRPQTVCERWKQSLIEHYGGKPGGEHYVPQCDQYGDFSPLQCHGNSGYCWCVDKEGREIEGSRTEPGMTPACIPTVAPPTMHPTPRPDVTPPATGTFLLYAQGQQIGYLPLNGTRLHKEKARTLLSLHGSIVVGIDYDCHEKMVYWTDVAGRTINRASLEPGAEPETIINTGLMSTEGLAIDYLRRTMFWTDSGLDKIESARLDGTERKVLFDTQLVNPRAITVDAVRGNLYWTDWNREAPKIESSFVDGSNRRILVNDNIGLPNGLTFDPFSKQVCWADAGTKKLECILSDGTGRRVIQSNLNYPFSVVVYANYFYHTDWRRDGVISLNKDTGHIVEEYLPEQRSHLYGVTAVYPYCPSGRK

Target ORF information:

RefSeq Version	XM_012967810.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nidogen 2 (nid2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967810.2

ORF Insert Sequence:

ATGTGGGACA GAACTCTGCA TCTGCTCCTG CCCTTGCACT GCCTGCTGGC CTTCTCTGCT 
GCTATATCCC GGCATGAACT CTTCCCCTAT GGGGAACTGA GGGGAGATGT GCTCCTGCAG 
GAGGGGGACG ATGAGACTTC TGAGGTGGTG AAACTCTCCC AACCGCTGCT CTTCTATGAA 
GCCCAATTCA GCAATTTATA TGTAGGGACC AATGGAATTA TTTCCACTCA GGACTTCCCC 
AGAGAAACTC AGTATGTGGA TGATGGCTTC CCTACAGACT TCCCTGTCAT TGCTCCATTC 
CTCTCAGATA TAGATACCAG CAATGGCAAA GGAAAGATTT TTTATCGGCA GGACAATTCT 
GAACAAGTTC TCAATCGGGC AGTCAGTGAG ATCCAGAGGG GTTTCCCAGA GACTTCATTT 
GTTCCTACTG ATGCCTTCCT TGTGACTTGG GAAAATGTTG GTGCCTACGA AGAGATGACC 
CGTCACTCTC CATCGTCTGA TAGGAGAAAC ACGTTCCAGG CGGTACTAGC ATATGATCAC 
TCTGATGCTT ACGCTATATT CCTTTATCCT GAAGATGGAC TCCAGTTTTT TGGTACTCGT 
CCTAAGGAAT CCTACAATGT GCACATTGAG CTGCCTGCCA GAGTGGGCTT TAGCCGATCA 
GAATCGGGAC CATACTACAG TGTAACAAGT AATGAGCAAT CCGTGAAGAA TTTGTATCAA 
ACTGGAAACA CTGGTGTCCC AGGGATTTGG GTCTTTCACA TTGGCAGCAT TTGGGAATTA 
GACAATGTAA TTCCAGCAAA ATTCTTTGGT GCTCCCACAA AGGAACAATC GCTTGAAAAT 
TCACATACAG GTTTCCCAGA ACACAGAATA TTTGAAAAGA ATGAAAGTGA AGACAGTATT 
GATTACCCAG AGTCCTTTTA TGAGGAGAAT GAAGATGATC TGGAAGATAA TACAAGTGAT 
GCCACACCAA CACATAATGC ACACCAAAAA GTTGATTCAG ACACTGGAAA TCAGGGATAC 
TTGGACATAG ATCAGGCTTC CAAACTGACC CATGCAGATG TTGAACAGGA AGCTACATCA 
TCTGTTGTGC TTTCCTCTTC ACATCAGAAT CCTGATTGGA CACCCTACAA ACTCAGTGAA 
TCTGATACTG ACAGAGATGG GCCATTGCAG GAACCTCAAG TTCAGACAGG GGAGGATACA 
GATTTGACAA GGGGAGACAC ATTACTGACT GAAGAGCTGC CATCCCATAA AGCACATAAT 
TTAACACCTC AAAATTCTGA AGATCTCTCA ATATATCCTG AAAGTGAAGT TCTCGTGGGC 
AGTCACCCAG AACAGGAAAA TGATTTTGAA AGAAAGCAGA TTGATGCTGA TCCTGGACTC 
CAAACTGATG TGTTTTCCTT TAACCCTGTG GGCAAGGAGA CATGTGAGAG GAACCACGGG 
AGATGTTCTA GGTTTGCTTT TTGTGCCGAT TACTCTGCTG GCTTCTGCTG TCATTGCCAG 
GAAGATTATT ATGGCAACGG AGTACATTGT TTGCCAAGAG GGGCTCCACA CCGTGTGAAT 
GGTAAAGTGA GCGGAAACAT CCTGGTCGGT CAAACACCTG TCAACTTCAT TGCTGCCGAT 
TTACACGCAT ACATTGTGGC AAATGATGGA AGAGCGTACA CAGCAATCAG CCACGTTCCT 
GATCCTGCAT CCTGGTCTTT AACACCTCTT GCTCCAATTG GGGGACTCTT TGGCTGGCTT 
TTTGCTTTGG AAAAACCAGG CTTTCAAAAT GGTTTTAGCT TTACCGGTGC CAAGTTTGTA 
CACAACATTG ATGTCACATT TTACCCGGGA GAAGAAACTG TTCATATTAC TCAAACAGCA 
GATGGCTTTG ATTCTGAGGG TTATATGAAT GTAAAGACAG CAATTCAGGG CAAAATTCCA 
TATATCCCAG AGACATCGAC CATCAAGCTG TCTCCTTATA CTGAACTGTA TCAATACTCT 
GGCTCAGTTG TAACTTCGAC AGCCTACAGG GAATACACAG TCATCTCAGA ATCAAACGAA 
GAACAAAAAC TGTCTTACCG GTTACGGCAA AATGTCACTT ACCAAGAGTG CAGCCATTCC 
CAGAAACAGC CTGTTAGTGC TCAAAAGCTT TATGTTGATC GTGTCTTTGC CCTTTACAAT 
AAAGAAGAGA AGGTCCTTCG TTATGCAGTT ACAAACTACA TTGGATCTCT GCATGATTCG 
ACTGGACAGG ACGATCTCAC AGTTAACCCG TGCTATGATG GTACCCACTT GTGTGACACA 
AGAGCTAAAT GCCAGCCTGG TAGTGGTCTA GAATACAGTT GTGTTTGTGC GTCTGGATAC 
CAAGGGGATG GAAGAGATTG CACTGATGTA AATGAATGTG AAGTTGGCTT CACCCGGTGC 
GGCCAGAACA CTGTTTGTGT CAACCTCCAG GGGAGTTACC GTTGTGAGTG CGCCAGTGGT 
TTCACTTTAT CTGGAGACGG GCACAACTGC ATCTTGGCTT CACTAATAAA CCCATGTGAA 
GATGGAAGTC ATACATGTAA CAGAGACACA TCACGCTGTG TTCCGCGTGG AGATGGTGTA 
TTCACATGTG AATGTTTCCC TGGATTTAAC AAGAGTGGAG AAGACTGTGT TGATGTTGAT 
GAATGCACTG AGCACAGATG TCATCCAGAT GCCTCATGTA CCAACACCCT GGGCTCTTTC 
TCCTGTCGGT GTAACAGTGG TTATGAAGGA GATGGCTTCC AGTGCACTCA AATCTTAGGT 
CCTGAACCCC TGAAAACCCC CTGTTTAGAA AAGCGCCAGC AGCTACACCC AAGGGGCCCC 
CGTCCAGCTG TGGGACAGTT TGTACCAGAG TGTGATGTAG AGGGAAATTA TGTTCCATTA 
CAGTGTCATG GCAGCACTGG CCATTGTTGG TGTGTGGACA AATTAGGTGA AGAGATTACT 
GGAACAAGAA CACCACCGGG CAGACCAGCC CCAAATTGTG GGGAAAGAGG TCCTGAACCC 
CTGAAAACCC CCTGTTTAGA AAGGCGCCAG CAGCTGCTTG GACAGCTGCA CCCAAGAGGC 
CCCCGTCCAG CTGTGGGACA GTTTGTACCT GAGTGTGATG CAGAGGGAAA TTATGTTTCA 
TTACAGTGTC ATGGCAGCAC TGGCCATTGT TGGTGTGTGG ACAAATTAGG TGAAGAGATT 
GCTGGAACAA GAACACCACC TGGCAGACCA GGCCCAAATT GTGGGGAAAC AGAGCCTACT 
CGGAGACCTC AGACAGTGTG TGAGAGGTGG AAGCAGAGTC TTATTGAGCA TTATGGTGGG 
AAACCAGGTG GAGAACATTA TGTGCCTCAG TGTGATCAAT ATGGTGACTT CAGTCCTCTC 
CAGTGCCATG GAAACAGTGG CTATTGCTGG TGTGTGGATA AAGAAGGAAG AGAAATTGAA 
GGATCTAGAA CAGAACCTGG AATGACTCCA GCATGTATAC CGACAGTGGC ACCTCCAACC 
ATGCATCCAA CTCCGCGTCC TGATGTGACC CCTCCTGCAA CTGGCACCTT CCTCTTGTAT 
GCTCAGGGTC AGCAGATTGG CTACTTACCA CTCAACGGCA CAAGGCTCCA TAAGGAAAAA 
GCCAGGACAC TGCTGTCACT TCATGGTTCT ATAGTGGTTG GTATTGACTA TGACTGCCAT 
GAGAAAATGG TGTACTGGAC GGATGTTGCA GGTCGTACAA TCAACCGCGC AAGCTTAGAA 
CCAGGTGCAG AACCGGAAAC TATCATTAAC ACAGGGTTGA TGAGCACAGA GGGCTTAGCC 
ATTGACTACC TTCGCAGGAC AATGTTTTGG ACAGATAGCG GCCTAGACAA GATTGAGAGT 
GCGCGGTTGG ACGGCACTGA GAGGAAGGTT CTATTTGACA CACAGCTTGT GAATCCTCGA 
GCCATCACTG TGGATGCGGT TAGAGGGAAC CTGTACTGGA CAGACTGGAA CAGAGAGGCT 
CCAAAGATTG AATCTTCGTT TGTAGACGGA TCAAACAGGA GGATTCTGGT TAATGACAAC 
ATCGGCCTTC CCAACGGTTT AACCTTTGAT CCCTTCTCCA AACAAGTCTG TTGGGCTGAT 
GCAGGAACCA AGAAGCTTGA ATGCATTTTG TCAGACGGAA CAGGGCGACG GGTCATACAG 
AGTAACTTGA ATTATCCTTT TAGTGTTGTT GTGTATGCAA ATTACTTCTA CCACACAGAC 
TGGAGGAGGG ATGGGGTAAT ATCCCTTAAC AAGGACACTG GGCACATTGT TGAAGAATAC 
CTTCCTGAGC AGCGATCCCA TCTCTATGGA GTAACAGCTG TGTACCCATA CTGCCCTTCA 
GGGAGAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nid2 ( XM_012967811.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823265.1 Xenopus tropicalis nidogen 2 (nid2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa29904
Clone ID Related Accession (Same CDS sequence)	XM_012967811.2
Accession Version	XM_012967811.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4320bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nidogen-2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012967811.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGACA GAACTCTGCA TCTGCTCCTG CCCTTGCACT GCCTGCTGGC CTTCTCTGCT 
GCTATATCCC GGCATGAACT CTTCCCCTAT GGGGAACTGA GGGGAGATGT GCTCCTGCAG 
GAGGGGGACG ATGAGACTTC TGAGGTGGTG AAACTCTCCC AACCGCTGCT CTTCTATGAA 
GCCCAATTCA GCAATTTATA TGTAGGGACC AATGGAATTA TTTCCACTCA GGACTTCCCC 
AGAGAAACTC AGTATGTGGA TGATGGCTTC CCTACAGACT TCCCTGTCAT TGCTCCATTC 
CTCTCAGATA TAGATACCAG CAATGGCAAA GGAAAGATTT TTTATCGGCA GGACAATTCT 
GAACAAGTTC TCAATCGGGC AGTCAGTGAG ATCCAGAGGG GTTTCCCAGA GACTTCATTT 
GTTCCTACTG ATGCCTTCCT TGTGACTTGG GAAAATGTTG GTGCCTACGA AGAGATGACC 
CGTCACTCTC CATCGTCTGA TAGGAGAAAC ACGTTCCAGG CGGTACTAGC ATATGATCAC 
TCTGATGCTT ACGCTATATT CCTTTATCCT GAAGATGGAC TCCAGTTTTT TGGTACTCGT 
CCTAAGGAAT CCTACAATGT GCACATTGAG CTGCCTGCCA GAGTGGGCTT TAGCCGATCA 
GAATCGGGAC CATACTACAG TGTAACAAGT AATGAGCAAT CCGTGAAGAA TTTGTATCAA 
ACTGGAAACA CTGGTGTCCC AGGGATTTGG GTCTTTCACA TTGGCAGCAT TTGGGAATTA 
GACAATGTAA TTCCAGCAAA ATTCTTTGGT GCTCCCACAA AGGAACAATC GCTTGAAAAT 
TCACATACAG GTTTCCCAGA ACACAGAATA TTTGAAAAGA ATGAAAGTGA AGACAGTATT 
GATTACCCAG AGTCCTTTTA TGAGGAGAAT GAAGATGATC TGGAAGATAA TACAAGTGAT 
GCCACACCAA CACATAATGC ACACCAAAAA GTTGATTCAG ACACTGGAAA TCAGGGATAC 
TTGGACATAG ATCAGGCTTC CAAACTGACC CATGCAGATG TTGAACAGGA AGCTACATCA 
TCTGTTGTGC TTTCCTCTTC ACATCAGAAT CCTGATTGGA CACCCTACAA ACTCAGTGAA 
TCTGATACTG ACAGAGATGG GCCATTGCAG GAACCTCAAG TTCAGACAGG GGAGGATACA 
GATTTGACAA GGGGAGACAC ATTACTGACT GAAGAGCTGC CATCCCATAA AGCACATAAT 
TTAACACCTC AAAATTCTGA AGATCTCTCA ATATATCCTG AAAGTGAAGT TCTCGTGGGC 
AGTCACCCAG AACAGGAAAA TGATTTTGAA AGAAAGCAGA TTGATGCTGA TCCTGGACTC 
CAAACTGATG TGTTTTCCTT TAACCCTGTG GGCAAGGAGA CATGTGAGAG GAACCACGGG 
AGATGTTCTA GGTTTGCTTT TTGTGCCGAT TACTCTGCTG GCTTCTGCTG TCATTGCCAG 
GAAGATTATT ATGGCAACGG AGTACATTGT TTGCCAAGAG GGGCTCCACA CCGTGTGAAT 
GGTAAAGTGA GCGGAAACAT CCTGGTCGGT CAAACACCTG TCAACTTCAT TGCTGCCGAT 
TTACACGCAT ACATTGTGGC AAATGATGGA AGAGCGTACA CAGCAATCAG CCACGTTCCT 
GATCCTGCAT CCTGGTCTTT AACACCTCTT GCTCCAATTG GGGGACTCTT TGGCTGGCTT 
TTTGCTTTGG AAAAACCAGG CTTTCAAAAT GGTTTTAGCT TTACCGGTGC CAAGTTTGTA 
CACAACATTG ATGTCACATT TTACCCGGGA GAAGAAACTG TTCATATTAC TCAAACAGCA 
GATGGCTTTG ATTCTGAGGG TTATATGAAT GTAAAGACAG CAATTCAGGG CAAAATTCCA 
TATATCCCAG AGACATCGAC CATCAAGCTG TCTCCTTATA CTGAACTGTA TCAATACTCT 
GGCTCAGTTG TAACTTCGAC AGCCTACAGG GAATACACAG TCATCTCAGA ATCAAACGAA 
GAACAAAAAC TGTCTTACCG GTTACGGCAA AATGTCACTT ACCAAGAGTG CAGCCATTCC 
CAGAAACAGC CTGTTAGTGC TCAAAAGCTT TATGTTGATC GTGTCTTTGC CCTTTACAAT 
AAAGAAGAGA AGGTCCTTCG TTATGCAGTT ACAAACTACA TTGGATCTCT GCATGATTCG 
ACTGGACAGG ACGATCTCAC AGTTAACCCG TGCTATGATG GTACCCACTT GTGTGACACA 
AGAGCTAAAT GCCAGCCTGG TAGTGGTCTA GAATACAGTT GTGTTTGTGC GTCTGGATAC 
CAAGGGGATG GAAGAGATTG CACTGATGTA AATGAATGTG AAGTTGGCTT CACCCGGTGC 
GGCCAGAACA CTGTTTGTGT CAACCTCCAG GGGAGTTACC GTTGTGAGTG CGCCAGTGGT 
TTCACTTTAT CTGGAGACGG GCACAACTGC ATCTTGGCTT CACTAATAAA CCCATGTGAA 
GATGGAAGTC ATACATGTAA CAGAGACACA TCACGCTGTG TTCCGCGTGG AGATGGTGTA 
TTCACATGTG AATGTTTCCC TGGATTTAAC AAGAGTGGAG AAGACTGTGT TGATGTTGAT 
GAATGCACTG AGCACAGATG TCATCCAGAT GCCTCATGTA CCAACACCCT GGGCTCTTTC 
TCCTGTCGGT GTAACAGTGG TTATGAAGGA GATGGCTTCC AGTGCACTCA AATCTTAGGT 
CCTGAACCCC TGAAAACCCC CTGTTTAGAA AAGCGCCAGC AGCTACACCC AAGGGGCCCC 
CGTCCAGCTG TGGGACAGTT TGTACCAGAG TGTGATGTAG AGGGAAATTA TGTTCCATTA 
CAGTGTCATG GCAGCACTGG CCATTGTTGG TGTGTGGACA AATTAGGTGA AGAGATTACT 
GGAACAAGAA CACCACCGGG CAGACCAGCC CCAAATTGTG GGGAAAGAGG TCCTGAACCC 
CTGAAAACCC CCTGTTTAGA AAAGCGCCAG CAGCTACACC CAAGGGGCCC CCGTCCAGCT 
GTGGGACAGT TTGTACCAGA GTGTGATGTA GAGGGAAATT ATGTTCCATT ACAGTGTCAT 
GGCAGCACTG GCCATTGTTG GTGTGTGGAC AAATTAGGTG AAGAGATTAC TGGAACAAGA 
ACACCACCGG GCAGACCAGC CCCAAATTGT GGGGAAACAG AGCCTACTCG GAGACCTCAG 
ACAGTGTGTG AGAGGTGGAA GCAGAGTCTT ATTGAGCATT ATGGTGGGAA ACCAGGTGGA 
GAACATTATG TGCCTCAGTG TGATCAATAT GGTGACTTCA GTCCTCTCCA GTGCCATGGA 
AACAGTGGCT ATTGCTGGTG TGTGGATAAA GAAGGAAGAG AAATTGAAGG ATCTAGAACA 
GAACCTGGAA TGACTCCAGC ATGTATACCG ACAGTGGCAC CTCCAACCAT GCATCCAACT 
CCGCGTCCTG ATGTGACCCC TCCTGCAACT GGCACCTTCC TCTTGTATGC TCAGGGTCAG 
CAGATTGGCT ACTTACCACT CAACGGCACA AGGCTCCATA AGGAAAAAGC CAGGACACTG 
CTGTCACTTC ATGGTTCTAT AGTGGTTGGT ATTGACTATG ACTGCCATGA GAAAATGGTG 
TACTGGACGG ATGTTGCAGG TCGTACAATC AACCGCGCAA GCTTAGAACC AGGTGCAGAA 
CCGGAAACTA TCATTAACAC AGGGTTGATG AGCACAGAGG GCTTAGCCAT TGACTACCTT 
CGCAGGACAA TGTTTTGGAC AGATAGCGGC CTAGACAAGA TTGAGAGTGC GCGGTTGGAC 
GGCACTGAGA GGAAGGTTCT ATTTGACACA CAGCTTGTGA ATCCTCGAGC CATCACTGTG 
GATGCGGTTA GAGGGAACCT GTACTGGACA GACTGGAACA GAGAGGCTCC AAAGATTGAA 
TCTTCGTTTG TAGACGGATC AAACAGGAGG ATTCTGGTTA ATGACAACAT CGGCCTTCCC 
AACGGTTTAA CCTTTGATCC CTTCTCCAAA CAAGTCTGTT GGGCTGATGC AGGAACCAAG 
AAGCTTGAAT GCATTTTGTC AGACGGAACA GGGCGACGGG TCATACAGAG TAACTTGAAT 
TATCCTTTTA GTGTTGTTGT GTATGCAAAT TACTTCTACC ACACAGACTG GAGGAGGGAT 
GGGGTAATAT CCCTTAACAA GGACACTGGG CACATTGTTG AAGAATACCT TCCTGAGCAG 
CGATCCCATC TCTATGGAGT AACAGCTGTG TACCCATACT GCCCTTCAGG GAGAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012823265.1
CDS	100..4419
Translation	MWDRTLHLLLPLHCLLAFSAAISRHELFPYGELRGDVLLQEGDDETSEVVKLSQPLLFYEAQFSNLYVGTNGIISTQDFPRETQYVDDGFPTDFPVIAPFLSDIDTSNGKGKIFYRQDNSEQVLNRAVSEIQRGFPETSFVPTDAFLVTWENVGAYEEMTRHSPSSDRRNTFQAVLAYDHSDAYAIFLYPEDGLQFFGTRPKESYNVHIELPARVGFSRSESGPYYSVTSNEQSVKNLYQTGNTGVPGIWVFHIGSIWELDNVIPAKFFGAPTKEQSLENSHTGFPEHRIFEKNESEDSIDYPESFYEENEDDLEDNTSDATPTHNAHQKVDSDTGNQGYLDIDQASKLTHADVEQEATSSVVLSSSHQNPDWTPYKLSESDTDRDGPLQEPQVQTGEDTDLTRGDTLLTEELPSHKAHNLTPQNSEDLSIYPESEVLVGSHPEQENDFERKQIDADPGLQTDVFSFNPVGKETCERNHGRCSRFAFCADYSAGFCCHCQEDYYGNGVHCLPRGAPHRVNGKVSGNILVGQTPVNFIAADLHAYIVANDGRAYTAISHVPDPASWSLTPLAPIGGLFGWLFALEKPGFQNGFSFTGAKFVHNIDVTFYPGEETVHITQTADGFDSEGYMNVKTAIQGKIPYIPETSTIKLSPYTELYQYSGSVVTSTAYREYTVISESNEEQKLSYRLRQNVTYQECSHSQKQPVSAQKLYVDRVFALYNKEEKVLRYAVTNYIGSLHDSTGQDDLTVNPCYDGTHLCDTRAKCQPGSGLEYSCVCASGYQGDGRDCTDVNECEVGFTRCGQNTVCVNLQGSYRCECASGFTLSGDGHNCILASLINPCEDGSHTCNRDTSRCVPRGDGVFTCECFPGFNKSGEDCVDVDECTEHRCHPDASCTNTLGSFSCRCNSGYEGDGFQCTQILGPEPLKTPCLEKRQQLHPRGPRPAVGQFVPECDVEGNYVPLQCHGSTGHCWCVDKLGEEITGTRTPPGRPAPNCGERGPEPLKTPCLEKRQQLHPRGPRPAVGQFVPECDVEGNYVPLQCHGSTGHCWCVDKLGEEITGTRTPPGRPAPNCGETEPTRRPQTVCERWKQSLIEHYGGKPGGEHYVPQCDQYGDFSPLQCHGNSGYCWCVDKEGREIEGSRTEPGMTPACIPTVAPPTMHPTPRPDVTPPATGTFLLYAQGQQIGYLPLNGTRLHKEKARTLLSLHGSIVVGIDYDCHEKMVYWTDVAGRTINRASLEPGAEPETIINTGLMSTEGLAIDYLRRTMFWTDSGLDKIESARLDGTERKVLFDTQLVNPRAITVDAVRGNLYWTDWNREAPKIESSFVDGSNRRILVNDNIGLPNGLTFDPFSKQVCWADAGTKKLECILSDGTGRRVIQSNLNYPFSVVVYANYFYHTDWRRDGVISLNKDTGHIVEEYLPEQRSHLYGVTAVYPYCPSGRK

Target ORF information:

RefSeq Version	XM_012967811.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nidogen 2 (nid2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967811.2

ORF Insert Sequence:

ATGTGGGACA GAACTCTGCA TCTGCTCCTG CCCTTGCACT GCCTGCTGGC CTTCTCTGCT 
GCTATATCCC GGCATGAACT CTTCCCCTAT GGGGAACTGA GGGGAGATGT GCTCCTGCAG 
GAGGGGGACG ATGAGACTTC TGAGGTGGTG AAACTCTCCC AACCGCTGCT CTTCTATGAA 
GCCCAATTCA GCAATTTATA TGTAGGGACC AATGGAATTA TTTCCACTCA GGACTTCCCC 
AGAGAAACTC AGTATGTGGA TGATGGCTTC CCTACAGACT TCCCTGTCAT TGCTCCATTC 
CTCTCAGATA TAGATACCAG CAATGGCAAA GGAAAGATTT TTTATCGGCA GGACAATTCT 
GAACAAGTTC TCAATCGGGC AGTCAGTGAG ATCCAGAGGG GTTTCCCAGA GACTTCATTT 
GTTCCTACTG ATGCCTTCCT TGTGACTTGG GAAAATGTTG GTGCCTACGA AGAGATGACC 
CGTCACTCTC CATCGTCTGA TAGGAGAAAC ACGTTCCAGG CGGTACTAGC ATATGATCAC 
TCTGATGCTT ACGCTATATT CCTTTATCCT GAAGATGGAC TCCAGTTTTT TGGTACTCGT 
CCTAAGGAAT CCTACAATGT GCACATTGAG CTGCCTGCCA GAGTGGGCTT TAGCCGATCA 
GAATCGGGAC CATACTACAG TGTAACAAGT AATGAGCAAT CCGTGAAGAA TTTGTATCAA 
ACTGGAAACA CTGGTGTCCC AGGGATTTGG GTCTTTCACA TTGGCAGCAT TTGGGAATTA 
GACAATGTAA TTCCAGCAAA ATTCTTTGGT GCTCCCACAA AGGAACAATC GCTTGAAAAT 
TCACATACAG GTTTCCCAGA ACACAGAATA TTTGAAAAGA ATGAAAGTGA AGACAGTATT 
GATTACCCAG AGTCCTTTTA TGAGGAGAAT GAAGATGATC TGGAAGATAA TACAAGTGAT 
GCCACACCAA CACATAATGC ACACCAAAAA GTTGATTCAG ACACTGGAAA TCAGGGATAC 
TTGGACATAG ATCAGGCTTC CAAACTGACC CATGCAGATG TTGAACAGGA AGCTACATCA 
TCTGTTGTGC TTTCCTCTTC ACATCAGAAT CCTGATTGGA CACCCTACAA ACTCAGTGAA 
TCTGATACTG ACAGAGATGG GCCATTGCAG GAACCTCAAG TTCAGACAGG GGAGGATACA 
GATTTGACAA GGGGAGACAC ATTACTGACT GAAGAGCTGC CATCCCATAA AGCACATAAT 
TTAACACCTC AAAATTCTGA AGATCTCTCA ATATATCCTG AAAGTGAAGT TCTCGTGGGC 
AGTCACCCAG AACAGGAAAA TGATTTTGAA AGAAAGCAGA TTGATGCTGA TCCTGGACTC 
CAAACTGATG TGTTTTCCTT TAACCCTGTG GGCAAGGAGA CATGTGAGAG GAACCACGGG 
AGATGTTCTA GGTTTGCTTT TTGTGCCGAT TACTCTGCTG GCTTCTGCTG TCATTGCCAG 
GAAGATTATT ATGGCAACGG AGTACATTGT TTGCCAAGAG GGGCTCCACA CCGTGTGAAT 
GGTAAAGTGA GCGGAAACAT CCTGGTCGGT CAAACACCTG TCAACTTCAT TGCTGCCGAT 
TTACACGCAT ACATTGTGGC AAATGATGGA AGAGCGTACA CAGCAATCAG CCACGTTCCT 
GATCCTGCAT CCTGGTCTTT AACACCTCTT GCTCCAATTG GGGGACTCTT TGGCTGGCTT 
TTTGCTTTGG AAAAACCAGG CTTTCAAAAT GGTTTTAGCT TTACCGGTGC CAAGTTTGTA 
CACAACATTG ATGTCACATT TTACCCGGGA GAAGAAACTG TTCATATTAC TCAAACAGCA 
GATGGCTTTG ATTCTGAGGG TTATATGAAT GTAAAGACAG CAATTCAGGG CAAAATTCCA 
TATATCCCAG AGACATCGAC CATCAAGCTG TCTCCTTATA CTGAACTGTA TCAATACTCT 
GGCTCAGTTG TAACTTCGAC AGCCTACAGG GAATACACAG TCATCTCAGA ATCAAACGAA 
GAACAAAAAC TGTCTTACCG GTTACGGCAA AATGTCACTT ACCAAGAGTG CAGCCATTCC 
CAGAAACAGC CTGTTAGTGC TCAAAAGCTT TATGTTGATC GTGTCTTTGC CCTTTACAAT 
AAAGAAGAGA AGGTCCTTCG TTATGCAGTT ACAAACTACA TTGGATCTCT GCATGATTCG 
ACTGGACAGG ACGATCTCAC AGTTAACCCG TGCTATGATG GTACCCACTT GTGTGACACA 
AGAGCTAAAT GCCAGCCTGG TAGTGGTCTA GAATACAGTT GTGTTTGTGC GTCTGGATAC 
CAAGGGGATG GAAGAGATTG CACTGATGTA AATGAATGTG AAGTTGGCTT CACCCGGTGC 
GGCCAGAACA CTGTTTGTGT CAACCTCCAG GGGAGTTACC GTTGTGAGTG CGCCAGTGGT 
TTCACTTTAT CTGGAGACGG GCACAACTGC ATCTTGGCTT CACTAATAAA CCCATGTGAA 
GATGGAAGTC ATACATGTAA CAGAGACACA TCACGCTGTG TTCCGCGTGG AGATGGTGTA 
TTCACATGTG AATGTTTCCC TGGATTTAAC AAGAGTGGAG AAGACTGTGT TGATGTTGAT 
GAATGCACTG AGCACAGATG TCATCCAGAT GCCTCATGTA CCAACACCCT GGGCTCTTTC 
TCCTGTCGGT GTAACAGTGG TTATGAAGGA GATGGCTTCC AGTGCACTCA AATCTTAGGT 
CCTGAACCCC TGAAAACCCC CTGTTTAGAA AAGCGCCAGC AGCTACACCC AAGGGGCCCC 
CGTCCAGCTG TGGGACAGTT TGTACCAGAG TGTGATGTAG AGGGAAATTA TGTTCCATTA 
CAGTGTCATG GCAGCACTGG CCATTGTTGG TGTGTGGACA AATTAGGTGA AGAGATTACT 
GGAACAAGAA CACCACCGGG CAGACCAGCC CCAAATTGTG GGGAAAGAGG TCCTGAACCC 
CTGAAAACCC CCTGTTTAGA AAAGCGCCAG CAGCTACACC CAAGGGGCCC CCGTCCAGCT 
GTGGGACAGT TTGTACCAGA GTGTGATGTA GAGGGAAATT ATGTTCCATT ACAGTGTCAT 
GGCAGCACTG GCCATTGTTG GTGTGTGGAC AAATTAGGTG AAGAGATTAC TGGAACAAGA 
ACACCACCGG GCAGACCAGC CCCAAATTGT GGGGAAACAG AGCCTACTCG GAGACCTCAG 
ACAGTGTGTG AGAGGTGGAA GCAGAGTCTT ATTGAGCATT ATGGTGGGAA ACCAGGTGGA 
GAACATTATG TGCCTCAGTG TGATCAATAT GGTGACTTCA GTCCTCTCCA GTGCCATGGA 
AACAGTGGCT ATTGCTGGTG TGTGGATAAA GAAGGAAGAG AAATTGAAGG ATCTAGAACA 
GAACCTGGAA TGACTCCAGC ATGTATACCG ACAGTGGCAC CTCCAACCAT GCATCCAACT 
CCGCGTCCTG ATGTGACCCC TCCTGCAACT GGCACCTTCC TCTTGTATGC TCAGGGTCAG 
CAGATTGGCT ACTTACCACT CAACGGCACA AGGCTCCATA AGGAAAAAGC CAGGACACTG 
CTGTCACTTC ATGGTTCTAT AGTGGTTGGT ATTGACTATG ACTGCCATGA GAAAATGGTG 
TACTGGACGG ATGTTGCAGG TCGTACAATC AACCGCGCAA GCTTAGAACC AGGTGCAGAA 
CCGGAAACTA TCATTAACAC AGGGTTGATG AGCACAGAGG GCTTAGCCAT TGACTACCTT 
CGCAGGACAA TGTTTTGGAC AGATAGCGGC CTAGACAAGA TTGAGAGTGC GCGGTTGGAC 
GGCACTGAGA GGAAGGTTCT ATTTGACACA CAGCTTGTGA ATCCTCGAGC CATCACTGTG 
GATGCGGTTA GAGGGAACCT GTACTGGACA GACTGGAACA GAGAGGCTCC AAAGATTGAA 
TCTTCGTTTG TAGACGGATC AAACAGGAGG ATTCTGGTTA ATGACAACAT CGGCCTTCCC 
AACGGTTTAA CCTTTGATCC CTTCTCCAAA CAAGTCTGTT GGGCTGATGC AGGAACCAAG 
AAGCTTGAAT GCATTTTGTC AGACGGAACA GGGCGACGGG TCATACAGAG TAACTTGAAT 
TATCCTTTTA GTGTTGTTGT GTATGCAAAT TACTTCTACC ACACAGACTG GAGGAGGGAT 
GGGGTAATAT CCCTTAACAA GGACACTGGG CACATTGTTG AAGAATACCT TCCTGAGCAG 
CGATCCCATC TCTATGGAGT AACAGCTGTG TACCCATACT GCCCTTCAGG GAGAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nid2 ( XM_018096080.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951569.1 Xenopus tropicalis nidogen 2 (nid2), transcript variant X2, mRNA.

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CloneID	OXa45925
Clone ID Related Accession (Same CDS sequence)	XM_018096080.1
Accession Version	XM_018096080.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4428bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nidogen-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGACA GAACTCTGCA TCTGCTCCTG CCCTTGCACT GCCTGCTGGC CTTCTCTGCT 
GCTATATCCC GGCATGAACT CTTCCCCTAT GGGGAACTGA GGGGAGATGT GCTCCTGCAG 
GAGGGGGACG ATGAGACTTC TGAGGTGGTG AAACTCTCCC AACCGCTGCT CTTCTATGAA 
GCCCAATTCA GCAATTTATA TGTAGGGACC AATGGAATTA TTTCCACTCA GGACTTCCCC 
AGAGAAACTC AGTATGTGGA TGATGGCTTC CCTACAGACT TCCCTGTCAT TGCTCCATTC 
CTCTCAGATA TAGATACCAG CAATGGCAAA GGAAAGATTT TTTATCGGCA GGACAATTCT 
GAACAAGTTC TCAATCGGGC AGTCAGTGAG ATCCAGAGGG GTTTCCCAGA GACTTCATTT 
GTTCCTACTG ATGCCTTCCT TGTGACTTGG GAAAATGTTG GTGCCTACGA AGAGATGACC 
CGTCACTCTC CATCGTCTGA TAGGAGAAAC ACGTTCCAGG CGGTACTAGC ATATGATCAC 
TCTGATGCTT ACGCTATATT CCTTTATCCT GAAGATGGAC TCCAGTTTTT TGGTACTCGT 
CCTAAGGAAT CCTACAATGT GCACATTGAG CTGCCTGCCA GAGTGGGCTT TAGCCGATCA 
GAATCGGGAC CATACTACAG TGTAACAAGT AATGAGCAAT CCGTGAAGAA TTTGTATCAA 
ACTGGAAACA CTGGTGTCCC AGGGATTTGG GTCTTTCACA TTGGCAGCAT TTGGGAATTA 
GACAATGTAA TTCCAGCAAA ATTCTTTGGT GCTCCCACAA AGGAACAATC GCTTGAAAAT 
TCACATACAG GTTTCCCAGA ACACAGAATA TTTGAAAAGA ATGAAAGTGA AGACAGTATT 
GATTACCCAG AGTCCTTTTA TGAGGAGAAT GAAGATGATC TGGAAGATAA TACAAGTGAT 
GCCACACCAA CACATAATGC ACACCAAAAA GTTGATTCAG ACACTGGAAA TCAGGGATAC 
TTGGACATAG ATCAGGCTTC CAAACTGACC CATGCAGATG TTGAACAGGA AGCTACATCA 
TCTGTTGTGC TTTCCTCTTC ACATCAGAAT CCTGATTGGA CACCCTACAA ACTCAGTGAA 
TCTGATACTG ACAGAGATGG GCCATTGCAG GAACCTCAAG TTCAGACAGG GGAGGATACA 
GATTTGACAA GGGGAGACAC ATTACTGACT GAAGAGCTGC CATCCCATAA AGCACATAAT 
TTAACACCTC AAAATTCTGA AGATCTCTCA ATATATCCTG AAAGTGAAGT TCTCGTGGGC 
AGTCACCCAG AACAGGAAAA TGATTTTGAA AGAAAGCAGA TTGATGCTGA TCCTGGACTC 
CAAACTGATG TGTTTTCCTT TAACCCTGTG GGCAAGGAGA CATGTGAGAG GAACCACGGG 
AGATGTTCTA GGTTTGCTTT TTGTGCCGAT TACTCTGCTG GCTTCTGCTG TCATTGCCAG 
GAAGATTATT ATGGCAACGG AGTACATTGT TTGCCAAGAG GGGCTCCACA CCGTGTGAAT 
GGTAAAGTGA GCGGAAACAT CCTGGTCGGT CAAACACCTG TCAACTTCAT TGCTGCCGAT 
TTACACGCAT ACATTGTGGC AAATGATGGA AGAGCGTACA CAGCAATCAG CCACGTTCCT 
GATCCTGCAT CCTGGTCTTT AACACCTCTT GCTCCAATTG GGGGACTCTT TGGCTGGCTT 
TTTGCTTTGG AAAAACCAGG CTTTCAAAAT GGTTTTAGCT TTACCGGTGC CAAGTTTGTA 
CACAACATTG ATGTCACATT TTACCCGGGA GAAGAAACTG TTCATATTAC TCAAACAGCA 
GATGGCTTTG ATTCTGAGGG TTATATGAAT GTAAAGACAG CAATTCAGGG CAAAATTCCA 
TATATCCCAG AGACATCGAC CATCAAGCTG TCTCCTTATA CTGAACTGTA TCAATACTCT 
GGCTCAGTTG TAACTTCGAC AGCCTACAGG GAATACACAG TCATCTCAGA ATCAAACGAA 
GAACAAAAAC TGTCTTACCG GTTACGGCAA AATGTCACTT ACCAAGAGTG CAGCCATTCC 
CAGAAACAGC CTGTTAGTGC TCAAAAGCTT TATGTTGATC GTGTCTTTGC CCTTTACAAT 
AAAGAAGAGA AGGTCCTTCG TTATGCAGTT ACAAACTACA TTGGATCTCT GCATGATTCG 
ACTGGACAGG ACGATCTCAC AGTTAACCCG TGCTATGATG GTACCCACTT GTGTGACACA 
AGAGCTAAAT GCCAGCCTGG TAGTGGTCTA GAATACAGTT GTGTTTGTGC GTCTGGATAC 
CAAGGGGATG GAAGAGATTG CACTGATGTA AATGAATGTG AAGTTGGCTT CACCCGGTGC 
GGCCAGAACA CTGTTTGTGT CAACCTCCAG GGGAGTTACC GTTGTGAGTG CGCCAGTGGT 
TTCACTTTAT CTGGAGACGG GCACAACTGC ATCTATGTTG ATGAATGCAC TGAGCACAGA 
TGTCATCCAG ATGCCTCATG TACCAACACC CTGGGCTCTT TCTCCTGTCG GTGTAACAGT 
GGTTATGAAG GAGATGGCTT CCAGTGCACT CAAATCTTAG GTCCTGAACC CCTGAAAACC 
CCCTGTTTAG AAAAGCGCCA GCAGCTACAC CCAAGGGGCC CCCGTCCAGC TGTGGGACAG 
TTTGTACCAG AGTGTGATGT AGAGGGAAAT TATGTTCCAT TACAGTGTCA TGGCAGCACT 
GGCCATTGTT GGTGTGTGGA CAAATTAGGT GAAGAGATTA CTGGAACAAG AACACCACCG 
GGCAGACCAG CCCCAAATTG TGGGGAAAGA GGTCCTGAAC CCCTGAAAAC CCCCTGTTTA 
GAAAAGCGCC AGCAGCTACA CCCAAGGGGC CCCCGTCCAG CTGTGGGACA GTTTGTACCA 
GAGTGTGATG TAGAGGGAAA TTATGTTCCA TTACAGTGTC ATGGCAGCAC TGGCCATTGT 
TGGTGTGTGG ACAAATTAGG TGAAGAGATT ACTGGAACAA GAACACCACC GGGCAGACCA 
GCCCCAAATT GTGGGGAAAC AGGTCCTGAA CCCCTGAAAA CCCCCTGTTT AGAAAGGCGC 
CAGCAGCTGC TTGGACAGCT GCACCCAAGA GGCCCCCGTC CAGCTGTGGG ACAGTTTGTA 
CCTGAGTGTG ATGCAGAGGG AAATTATGTT TCATTACAGT GTCATGGCAG CACTGGCCAT 
TGTTGGTGTG TGGACAAATT AGGTGAAGAG ATTGCTGGAA CAAGAACACC ACCTGGCAGA 
CCAGGCCCAA ATTGTGGGGA AACAGCAGAG CCTACTCGGA GACCTCAGAC AGTGTGTGAG 
AGGTGGAAGC AGAGTCTTAT TGAGCATTAT GGTGGGAAAC CAGGTGGAGA ACATTATGTG 
CCTCAGTGTG ATCAATATGG TGACTTCAGT CCTCTCCAGT GCCATGGAAA CAGTGGCTAT 
TGCTGGTGTG TGGATAAAGA AGGAAGAGAA ATTGAAGGAT CTAGAACAGA ACCTGGAATG 
ACTCCAGCAT GTATACCGAC AGTGGCACCT CCAACCATGC ATCCAACTCC GCGTCCTGAT 
GTGACCCCTC CTGCAACTGG CACCTTCCTC TTGTATGCTC AGGGTCAGCA GATTGGCTAC 
TTACCACTCA ACGGCACAAG GCTCCATAAG GAAAAAGCCA GGACACTGCT GTCACTTCAT 
GGTTCTATAG TGGTTGGTAT TGACTATGAC TGCCATGAGA AAATGGTGTA CTGGACGGAT 
GTTGCAGGTC GTACAATCAA CCGCGCAAGC TTAGAACCAG GTGCAGAACC GGAAACTATC 
ATTAACACAG GGTTGATGAG CACAGAGGGC TTAGCCATTG ACTACCTTCG CAGGACAATG 
TTTTGGACAG ATAGCGGCCT AGACAAGATT GAGAGTGCGC GGTTGGACGG CACTGAGAGG 
AAGGTTCTAT TTGACACACA GCTTGTGAAT CCTCGAGCCA TCACTGTGGA TGCGGTTAGA 
GGGAACCTGT ACTGGACAGA CTGGAACAGA GAGGCTCCAA AGATTGAATC TTCGTTTGTA 
GACGGATCAA ACAGGAGGAT TCTGGTTAAT GACAACATCG GCCTTCCCAA CGGTTTAACC 
TTTGATCCCT TCTCCAAACA AGTCTGTTGG GCTGATGCAG GAACCAAGAA GCTTGAATGC 
ATTTTGTCAG ACGGAACAGG GCGACGGGTC ATACAGAGTA ACTTGAATTA TCCTTTTAGT 
GTTGTTGTGT ATGCAAATTA CTTCTACCAC ACAGACTGGA GGAGGGATGG GGTAATATCC 
CTTAACAAGG ACACTGGGCA CATTGTTGAA GAATACCTTC CTGAGCAGCG ATCCCATCTC 
TATGGAGTAA CAGCTGTGTA CCCATACTGC CCTTCAGGGA GAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951569.1
CDS	100..4527
Translation	MWDRTLHLLLPLHCLLAFSAAISRHELFPYGELRGDVLLQEGDDETSEVVKLSQPLLFYEAQFSNLYVGTNGIISTQDFPRETQYVDDGFPTDFPVIAPFLSDIDTSNGKGKIFYRQDNSEQVLNRAVSEIQRGFPETSFVPTDAFLVTWENVGAYEEMTRHSPSSDRRNTFQAVLAYDHSDAYAIFLYPEDGLQFFGTRPKESYNVHIELPARVGFSRSESGPYYSVTSNEQSVKNLYQTGNTGVPGIWVFHIGSIWELDNVIPAKFFGAPTKEQSLENSHTGFPEHRIFEKNESEDSIDYPESFYEENEDDLEDNTSDATPTHNAHQKVDSDTGNQGYLDIDQASKLTHADVEQEATSSVVLSSSHQNPDWTPYKLSESDTDRDGPLQEPQVQTGEDTDLTRGDTLLTEELPSHKAHNLTPQNSEDLSIYPESEVLVGSHPEQENDFERKQIDADPGLQTDVFSFNPVGKETCERNHGRCSRFAFCADYSAGFCCHCQEDYYGNGVHCLPRGAPHRVNGKVSGNILVGQTPVNFIAADLHAYIVANDGRAYTAISHVPDPASWSLTPLAPIGGLFGWLFALEKPGFQNGFSFTGAKFVHNIDVTFYPGEETVHITQTADGFDSEGYMNVKTAIQGKIPYIPETSTIKLSPYTELYQYSGSVVTSTAYREYTVISESNEEQKLSYRLRQNVTYQECSHSQKQPVSAQKLYVDRVFALYNKEEKVLRYAVTNYIGSLHDSTGQDDLTVNPCYDGTHLCDTRAKCQPGSGLEYSCVCASGYQGDGRDCTDVNECEVGFTRCGQNTVCVNLQGSYRCECASGFTLSGDGHNCIYVDECTEHRCHPDASCTNTLGSFSCRCNSGYEGDGFQCTQILGPEPLKTPCLEKRQQLHPRGPRPAVGQFVPECDVEGNYVPLQCHGSTGHCWCVDKLGEEITGTRTPPGRPAPNCGERGPEPLKTPCLEKRQQLHPRGPRPAVGQFVPECDVEGNYVPLQCHGSTGHCWCVDKLGEEITGTRTPPGRPAPNCGETGPEPLKTPCLERRQQLLGQLHPRGPRPAVGQFVPECDAEGNYVSLQCHGSTGHCWCVDKLGEEIAGTRTPPGRPGPNCGETAEPTRRPQTVCERWKQSLIEHYGGKPGGEHYVPQCDQYGDFSPLQCHGNSGYCWCVDKEGREIEGSRTEPGMTPACIPTVAPPTMHPTPRPDVTPPATGTFLLYAQGQQIGYLPLNGTRLHKEKARTLLSLHGSIVVGIDYDCHEKMVYWTDVAGRTINRASLEPGAEPETIINTGLMSTEGLAIDYLRRTMFWTDSGLDKIESARLDGTERKVLFDTQLVNPRAITVDAVRGNLYWTDWNREAPKIESSFVDGSNRRILVNDNIGLPNGLTFDPFSKQVCWADAGTKKLECILSDGTGRRVIQSNLNYPFSVVVYANYFYHTDWRRDGVISLNKDTGHIVEEYLPEQRSHLYGVTAVYPYCPSGRK

Target ORF information:

RefSeq Version	XM_018096080.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nidogen 2 (nid2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096080.1

ORF Insert Sequence:

ATGTGGGACA GAACTCTGCA TCTGCTCCTG CCCTTGCACT GCCTGCTGGC CTTCTCTGCT 
GCTATATCCC GGCATGAACT CTTCCCCTAT GGGGAACTGA GGGGAGATGT GCTCCTGCAG 
GAGGGGGACG ATGAGACTTC TGAGGTGGTG AAACTCTCCC AACCGCTGCT CTTCTATGAA 
GCCCAATTCA GCAATTTATA TGTAGGGACC AATGGAATTA TTTCCACTCA GGACTTCCCC 
AGAGAAACTC AGTATGTGGA TGATGGCTTC CCTACAGACT TCCCTGTCAT TGCTCCATTC 
CTCTCAGATA TAGATACCAG CAATGGCAAA GGAAAGATTT TTTATCGGCA GGACAATTCT 
GAACAAGTTC TCAATCGGGC AGTCAGTGAG ATCCAGAGGG GTTTCCCAGA GACTTCATTT 
GTTCCTACTG ATGCCTTCCT TGTGACTTGG GAAAATGTTG GTGCCTACGA AGAGATGACC 
CGTCACTCTC CATCGTCTGA TAGGAGAAAC ACGTTCCAGG CGGTACTAGC ATATGATCAC 
TCTGATGCTT ACGCTATATT CCTTTATCCT GAAGATGGAC TCCAGTTTTT TGGTACTCGT 
CCTAAGGAAT CCTACAATGT GCACATTGAG CTGCCTGCCA GAGTGGGCTT TAGCCGATCA 
GAATCGGGAC CATACTACAG TGTAACAAGT AATGAGCAAT CCGTGAAGAA TTTGTATCAA 
ACTGGAAACA CTGGTGTCCC AGGGATTTGG GTCTTTCACA TTGGCAGCAT TTGGGAATTA 
GACAATGTAA TTCCAGCAAA ATTCTTTGGT GCTCCCACAA AGGAACAATC GCTTGAAAAT 
TCACATACAG GTTTCCCAGA ACACAGAATA TTTGAAAAGA ATGAAAGTGA AGACAGTATT 
GATTACCCAG AGTCCTTTTA TGAGGAGAAT GAAGATGATC TGGAAGATAA TACAAGTGAT 
GCCACACCAA CACATAATGC ACACCAAAAA GTTGATTCAG ACACTGGAAA TCAGGGATAC 
TTGGACATAG ATCAGGCTTC CAAACTGACC CATGCAGATG TTGAACAGGA AGCTACATCA 
TCTGTTGTGC TTTCCTCTTC ACATCAGAAT CCTGATTGGA CACCCTACAA ACTCAGTGAA 
TCTGATACTG ACAGAGATGG GCCATTGCAG GAACCTCAAG TTCAGACAGG GGAGGATACA 
GATTTGACAA GGGGAGACAC ATTACTGACT GAAGAGCTGC CATCCCATAA AGCACATAAT 
TTAACACCTC AAAATTCTGA AGATCTCTCA ATATATCCTG AAAGTGAAGT TCTCGTGGGC 
AGTCACCCAG AACAGGAAAA TGATTTTGAA AGAAAGCAGA TTGATGCTGA TCCTGGACTC 
CAAACTGATG TGTTTTCCTT TAACCCTGTG GGCAAGGAGA CATGTGAGAG GAACCACGGG 
AGATGTTCTA GGTTTGCTTT TTGTGCCGAT TACTCTGCTG GCTTCTGCTG TCATTGCCAG 
GAAGATTATT ATGGCAACGG AGTACATTGT TTGCCAAGAG GGGCTCCACA CCGTGTGAAT 
GGTAAAGTGA GCGGAAACAT CCTGGTCGGT CAAACACCTG TCAACTTCAT TGCTGCCGAT 
TTACACGCAT ACATTGTGGC AAATGATGGA AGAGCGTACA CAGCAATCAG CCACGTTCCT 
GATCCTGCAT CCTGGTCTTT AACACCTCTT GCTCCAATTG GGGGACTCTT TGGCTGGCTT 
TTTGCTTTGG AAAAACCAGG CTTTCAAAAT GGTTTTAGCT TTACCGGTGC CAAGTTTGTA 
CACAACATTG ATGTCACATT TTACCCGGGA GAAGAAACTG TTCATATTAC TCAAACAGCA 
GATGGCTTTG ATTCTGAGGG TTATATGAAT GTAAAGACAG CAATTCAGGG CAAAATTCCA 
TATATCCCAG AGACATCGAC CATCAAGCTG TCTCCTTATA CTGAACTGTA TCAATACTCT 
GGCTCAGTTG TAACTTCGAC AGCCTACAGG GAATACACAG TCATCTCAGA ATCAAACGAA 
GAACAAAAAC TGTCTTACCG GTTACGGCAA AATGTCACTT ACCAAGAGTG CAGCCATTCC 
CAGAAACAGC CTGTTAGTGC TCAAAAGCTT TATGTTGATC GTGTCTTTGC CCTTTACAAT 
AAAGAAGAGA AGGTCCTTCG TTATGCAGTT ACAAACTACA TTGGATCTCT GCATGATTCG 
ACTGGACAGG ACGATCTCAC AGTTAACCCG TGCTATGATG GTACCCACTT GTGTGACACA 
AGAGCTAAAT GCCAGCCTGG TAGTGGTCTA GAATACAGTT GTGTTTGTGC GTCTGGATAC 
CAAGGGGATG GAAGAGATTG CACTGATGTA AATGAATGTG AAGTTGGCTT CACCCGGTGC 
GGCCAGAACA CTGTTTGTGT CAACCTCCAG GGGAGTTACC GTTGTGAGTG CGCCAGTGGT 
TTCACTTTAT CTGGAGACGG GCACAACTGC ATCTATGTTG ATGAATGCAC TGAGCACAGA 
TGTCATCCAG ATGCCTCATG TACCAACACC CTGGGCTCTT TCTCCTGTCG GTGTAACAGT 
GGTTATGAAG GAGATGGCTT CCAGTGCACT CAAATCTTAG GTCCTGAACC CCTGAAAACC 
CCCTGTTTAG AAAAGCGCCA GCAGCTACAC CCAAGGGGCC CCCGTCCAGC TGTGGGACAG 
TTTGTACCAG AGTGTGATGT AGAGGGAAAT TATGTTCCAT TACAGTGTCA TGGCAGCACT 
GGCCATTGTT GGTGTGTGGA CAAATTAGGT GAAGAGATTA CTGGAACAAG AACACCACCG 
GGCAGACCAG CCCCAAATTG TGGGGAAAGA GGTCCTGAAC CCCTGAAAAC CCCCTGTTTA 
GAAAAGCGCC AGCAGCTACA CCCAAGGGGC CCCCGTCCAG CTGTGGGACA GTTTGTACCA 
GAGTGTGATG TAGAGGGAAA TTATGTTCCA TTACAGTGTC ATGGCAGCAC TGGCCATTGT 
TGGTGTGTGG ACAAATTAGG TGAAGAGATT ACTGGAACAA GAACACCACC GGGCAGACCA 
GCCCCAAATT GTGGGGAAAC AGGTCCTGAA CCCCTGAAAA CCCCCTGTTT AGAAAGGCGC 
CAGCAGCTGC TTGGACAGCT GCACCCAAGA GGCCCCCGTC CAGCTGTGGG ACAGTTTGTA 
CCTGAGTGTG ATGCAGAGGG AAATTATGTT TCATTACAGT GTCATGGCAG CACTGGCCAT 
TGTTGGTGTG TGGACAAATT AGGTGAAGAG ATTGCTGGAA CAAGAACACC ACCTGGCAGA 
CCAGGCCCAA ATTGTGGGGA AACAGCAGAG CCTACTCGGA GACCTCAGAC AGTGTGTGAG 
AGGTGGAAGC AGAGTCTTAT TGAGCATTAT GGTGGGAAAC CAGGTGGAGA ACATTATGTG 
CCTCAGTGTG ATCAATATGG TGACTTCAGT CCTCTCCAGT GCCATGGAAA CAGTGGCTAT 
TGCTGGTGTG TGGATAAAGA AGGAAGAGAA ATTGAAGGAT CTAGAACAGA ACCTGGAATG 
ACTCCAGCAT GTATACCGAC AGTGGCACCT CCAACCATGC ATCCAACTCC GCGTCCTGAT 
GTGACCCCTC CTGCAACTGG CACCTTCCTC TTGTATGCTC AGGGTCAGCA GATTGGCTAC 
TTACCACTCA ACGGCACAAG GCTCCATAAG GAAAAAGCCA GGACACTGCT GTCACTTCAT 
GGTTCTATAG TGGTTGGTAT TGACTATGAC TGCCATGAGA AAATGGTGTA CTGGACGGAT 
GTTGCAGGTC GTACAATCAA CCGCGCAAGC TTAGAACCAG GTGCAGAACC GGAAACTATC 
ATTAACACAG GGTTGATGAG CACAGAGGGC TTAGCCATTG ACTACCTTCG CAGGACAATG 
TTTTGGACAG ATAGCGGCCT AGACAAGATT GAGAGTGCGC GGTTGGACGG CACTGAGAGG 
AAGGTTCTAT TTGACACACA GCTTGTGAAT CCTCGAGCCA TCACTGTGGA TGCGGTTAGA 
GGGAACCTGT ACTGGACAGA CTGGAACAGA GAGGCTCCAA AGATTGAATC TTCGTTTGTA 
GACGGATCAA ACAGGAGGAT TCTGGTTAAT GACAACATCG GCCTTCCCAA CGGTTTAACC 
TTTGATCCCT TCTCCAAACA AGTCTGTTGG GCTGATGCAG GAACCAAGAA GCTTGAATGC 
ATTTTGTCAG ACGGAACAGG GCGACGGGTC ATACAGAGTA ACTTGAATTA TCCTTTTAGT 
GTTGTTGTGT ATGCAAATTA CTTCTACCAC ACAGACTGGA GGAGGGATGG GGTAATATCC 
CTTAACAAGG ACACTGGGCA CATTGTTGAA GAATACCTTC CTGAGCAGCG ATCCCATCTC 
TATGGAGTAA CAGCTGTGTA CCCATACTGC CCTTCAGGGA GAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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nid2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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