herc4 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	herc4
Entrez Gene ID	100036667
Full Name	HECT and RLD domain containing E3 ubiquitin protein ligase 4
General protein information	Preferred Names HECT and RLD domain containing E3 ubiquitin protein ligase 4 Names probable E3 ubiquitin-protein ligase HERC4 hect domain and RLD 4
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012965815.1	XP_012821269.1	probable E3 ubiquitin-protein ligase HERC4 isoform X1
NM_001128650.1	NP_001122122.1	probable E3 ubiquitin-protein ligase HERC4
XM_031904674.1	XP_031760534.1	probable E3 ubiquitin-protein ligase HERC4 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	HERC4	NP_071362.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006994	XP_308776.4
Canis lupus familiaris (dog)	HERC4	XP_005618991.1
Danio rerio (zebrafish)	herc4	XP_685685.5
Drosophila melanogaster (fruit fly)	CG9153	NP_001261249.1
Bos taurus (cattle)	HERC4	NP_001070362.2
Pan troglodytes (chimpanzee)	HERC4	XP_001167753.1
Mus musculus (house mouse)	Herc4	NP_084390.1
Rattus norvegicus (Norway rat)	Herc4	NP_001012074.1
Gallus gallus (chicken)	HERC4	XP_004942077.1
Arabidopsis thaliana (thale cress)	UVR8	NP_201191.1
Oryza sativa (rice)	Os02g0554100	NP_001047115.1
Xenopus tropicalis (tropical clawed frog)	herc4	NP_001122122.1
	HERC4	XP_001087608.1
Oryza sativa (rice)	Os04g0435700	NP_001052849.1

Gene Ontology
Bibliography

Related articles in PubMed

Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Strausberg RL, Feingold EA, Grouse LH, Derge JG, Klausner RD, Collins FS, Wagner L, Shenmen CM, Schuler GD, Altschul SF, Zeeberg B, Buetow KH, Schaefer CF, Bhat NK, Hopkins RF, Jordan H, Moore T, Max SI, Wang J, Hsieh F, Diatchenko L, Marusina K, Farmer AA, Rubin GM, Hong L, Stapleton M, Soares MB, Bonaldo MF, Casavant TL, Scheetz TE, Brownstein MJ, Usdin TB, Toshiyuki S, Carninci P, Prange C, Raha SS, Loquellano NA, Peters GJ, Abramson RD, Mullahy SJ, Bosak SA, McEwan PJ, McKernan KJ, Malek JA, Gunaratne PH, Richards S, Worley KC, Hale S, Garcia AM, Gay LJ, Hulyk SW, Villalon DK, Muzny DM, Sodergren EJ, Lu X, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madan A, Young AC, Shevchenko Y, Bouffard GG, Blakesley RW, Touchman JW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Krzywinski MI, Skalska U, Smailus DE, Schnerch A, Schein JE, Jones SJ, Marra MA,
Proceedings of the National Academy of Sciences of the United States of America99(26)16899-903(2002 Dec)

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following herc4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the herc4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa61082	XM_031904674.1 Latest version!	Xenopus tropicalis HECT and RLD domain containing E3 ubiquitin protein ligase 4 (herc4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa21746	NM_001128650.1 Latest version!	Xenopus tropicalis HECT and RLD domain containing E3 ubiquitin protein ligase 4 (herc4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa29858	XM_012965815.1 Latest version!	Xenopus tropicalis HECT and RLD domain containing E3 ubiquitin protein ligase 4 (herc4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

herc4 ( XM_031904674.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031760534.1 Xenopus tropicalis HECT and RLD domain containing E3 ubiquitin protein ligase 4 (herc4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa61082
Clone ID Related Accession (Same CDS sequence)	XM_031904674.1
Accession Version	XM_031904674.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3174bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATGCT GGGGAAATAC ATCTTTTGGA CAGCTGGGAT TAGGGGGGAT TGATGAGGAG 
GTTGTTTTAG AACCGAGAAG ATGTGACTTT TTCATCAATA AAAATGTCCG TGATGTCGGA 
TGTGGAAGCA GACACACAGT TTTTGTCTTG GATGATGGGA CAGTTTACAC CTGCGGATGT 
AATGATCTTG GGCAACTGGG ACATGACAAA AGCAGAAGAA AGCCAGAGCA GGTTGGAGCC 
CTGGATGCTC AGATCATTGT GGCAGTTTCA TGTGGAGAAG CTCACACCTT GGCATTAAAT 
GACAAAGGTC AAGTGTTTTC TTGGGGTCAT GCTGTATATG GACAGATAGG TCTCTCAACT 
GCAGAAGAAT ATATTAGAGT ACCCAGAAAT ATAAAGAGTC TGTCTGATGT ACAGATTGTG 
CAAGTTGCCT GTGGTCATCA TCATTCACTT GCACTGTCCA AAGACAGTGA TATTTTTTCA 
TGGGGACAAA ACCAATATGG CCAGCTTGGC TTGGGTTTAG AAATAAAGAA AGAATGTGCA 
CCCAGACATA TTAAGTCTCT ATCAGGGATT CCCTTCGCTC ATATTGCTGC TGGAGGTGGA 
CACAGTTTTG CGTTGACAAT CTCAGGGGCC ATTTTCGGTT GGGGAAGAAA CAAATTTGGC 
CAGCTTGGTC TTACTGATGA AACTGATCGA AATGTCCCAG CTCTTCTCAA GTCACTTCGC 
TCACAGAAAA TCATTCATAT TTGTTGTGGA GAAGATCATA CAGCTGCTTT AACCAAAGAG 
GGTGGTGTAT TTACATTTGG AGCTGGGGGC TATGGACAGC TTGGTCATAA CTCTACCAAC 
CATGAAGTCA ACCCAAGGAA AGTGTTTGAG CTTATGGGAA GCATTGTGAC ACAAATCGCT 
TGTGGGAGGC AGCACACATT AGCTTTTGTT CCTTCATCAG GACGCATTTA TTCGTTTGGG 
CTTGGTGGCA ATGGGCAGTT AGGAACAGGG TCTACCATTA ACAGAAAAAG TCCTTTCACA 
GTTAAAGGGA ACTGGCTGAC TTATGAAGAC TATGTTCGGC ACCTTCCTGA GGCTAATGGA 
TGTTACTGTT TGAAGAGAAT TTACAGCGGT GGAGACCAGA GTTTTGCACA TTATATTACA 
ATACAGAATT GCCATCCTCC AGATGACTTT AGGAGGTGTA ATTGTTCCAG AAAAATACGG 
ACTTTAGACG AGGGAGTTAT ACAACGATGG CTGAATTACT CCTTGGGAAG ACTTCCATTA 
GAAATAGTAA ATGAAATAGA CAAAGTATTT TCATCAGCTG CATGTTTAAA TGGAAGCTTT 
TTAGTGACAA GCAACAACGA TCACTATATG ACAAGTTCCA AGTTCTCAGG AGTAGACATG 
AATGTTGCTA GACTTCTTCT TCACAAGCTT ATGGATCCAT CACAGCCACA AATAGCCCAA 
CAGGTGGCTG CCAGCTTGGA GAAGAATCTT ATTCCCAGAC TCACAAGTTC TCCCCCCGAC 
ATTGAGTGCT TGCGTTTGTA TCTCATCCTC CCAGAGTGTC CCCCCATGGC TGATGCCAAC 
AACTTAACTT CTTTAGCAAT TCCGTTTGGC ACAGCTATTG TAAACTTGGA AAAAGCACCA 
TTAAAAGTTT TAGAAAACTG GTGGTCCCTA CTTGAGCCTC CGCTTTTCTT GAAAGTAGTA 
CAACTTTATA AGGATGTTGT GGTGCACCTA TTGAAACTCT CCAAAAATGG AATTCCTCTT 
ACGGAGCGAA GATTATTCTA CAGATTTCTT AATACTGCCC TCAGGGTTTT AGAGATCCTG 
CACAGGGTAA ATGAAAAAGC TGCACAGATC ATACCCTATG ATAAATTCTA CATTCATGAA 
GTGCAAGATT TTGTAGATAT CCAAAACGAC TACATTAACT GGATTAAGCA ACAAGCATCT 
GGAATGGATG TGAATCATGG ATTATATGAG GTAGCTGATC CACCAGTTAC AATCTGCACA 
TACCCCTTTG TTTTTGATGC TCAGGCAAAG ACAACACTTC TGCAGACAGA TGCAGTCATT 
CAGATGCAGA TGGCAGTTGA CAATGCTCAT AGGCAAAACA ACTTATCCTG GTTATTTCCT 
GCAACTGAAT CAGTGGATCC TTGTCTGATA TTAGTTGTTC GCAGAGATAA TATTGTTGGT 
GATGCTATGG AAGTCCTAAG GAAAACCAAA AATATAGACT ACAAAAAGCC AATGCGGGTA 
ATCTTTTTTG GAGAAGAAGC AATTGATGCA GGTGGCGTTC GTAAGGAGTT TTTCCTACTC 
ATCATGAGAG AATTACTGGA CCCCAAGTAT GGAATGTTCA GATATTACGA AGAATCCAGA 
TTAATCTGGT TTTCTGATAA GACCTTTGAA GACAGTGATT TATTCCACCT TATTGGTGTA 
GTCTGTGGTT TAGCAATATA CAATTTCACG ATTATTGAGC TTCACTTCCC TTTGGCTTTA 
TATAAGAAGC TTTTAAAAAA GAAACCATCC TTGGAAGACT TAAAGGAGCT GATGCCTATT 
ATCGGACGGA GCATGCAGCA GTTGTTGGAC TATACTGAAG ATGACATTGA AGAGACATTC 
TGCCTCAATT TTACGATCAC AGTAGAAAAC TTCGGAACAA CAGAAGTCAG AGAGCTAATC 
CCAAATGGAG CTGAGATACC AGTGAATAGG AAAAACAGGC AGGACTTCGT AGATGCCTAT 
GTAGACTACA TCTTCAATCA GTCTGTGGCT TCCTTATTCA GTGCGTTTAG TGCAGGATTT 
CACAAAGTGT GTGGTAGCAA GGTACTCGAG CTCTTCCAGC CCAATGAACT GCAGGCCATG 
GTGATTGGCA ATACCGCCTA TGACTGGAAG GAACTCGAAA AAAGCACAGA ATACAAAGGA 
GAGTATTGGG CCGACCACCC TACAATTAAG ATGTTCTGGG AAATTTTTCA TGCATTACCC 
TTGGAAAAGA AAAAACAATT CTTGCTATTC CTTACTGGCA GCGATCGGAT TCCTATTCTC 
GGTATGAAAA GTTTAAAGCT TGTCATTCAG CCAACAGGAG GAGGAGAACA GTTCCTACCA 
GTCGCTCACA CCTGCTTCAA CCTCTTGGAT CTCCCCAAAT ACAGCAAGAA AGAAACTCTG 
CGATCCAAAC TGATTCAAGC TATAGATCAC TATGAAGGCT TTAGCCTCGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031760534.1
CDS	257..3430
Translation	MLCWGNTSFGQLGLGGIDEEVVLEPRRCDFFINKNVRDVGCGSRHTVFVLDDGTVYTCGCNDLGQLGHDKSRRKPEQVGALDAQIIVAVSCGEAHTLALNDKGQVFSWGHAVYGQIGLSTAEEYIRVPRNIKSLSDVQIVQVACGHHHSLALSKDSDIFSWGQNQYGQLGLGLEIKKECAPRHIKSLSGIPFAHIAAGGGHSFALTISGAIFGWGRNKFGQLGLTDETDRNVPALLKSLRSQKIIHICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTNHEVNPRKVFELMGSIVTQIACGRQHTLAFVPSSGRIYSFGLGGNGQLGTGSTINRKSPFTVKGNWLTYEDYVRHLPEANGCYCLKRIYSGGDQSFAHYITIQNCHPPDDFRRCNCSRKIRTLDEGVIQRWLNYSLGRLPLEIVNEIDKVFSSAACLNGSFLVTSNNDHYMTSSKFSGVDMNVARLLLHKLMDPSQPQIAQQVAASLEKNLIPRLTSSPPDIECLRLYLILPECPPMADANNLTSLAIPFGTAIVNLEKAPLKVLENWWSLLEPPLFLKVVQLYKDVVVHLLKLSKNGIPLTERRLFYRFLNTALRVLEILHRVNEKAAQIIPYDKFYIHEVQDFVDIQNDYINWIKQQASGMDVNHGLYEVADPPVTICTYPFVFDAQAKTTLLQTDAVIQMQMAVDNAHRQNNLSWLFPATESVDPCLILVVRRDNIVGDAMEVLRKTKNIDYKKPMRVIFFGEEAIDAGGVRKEFFLLIMRELLDPKYGMFRYYEESRLIWFSDKTFEDSDLFHLIGVVCGLAIYNFTIIELHFPLALYKKLLKKKPSLEDLKELMPIIGRSMQQLLDYTEDDIEETFCLNFTITVENFGTTEVRELIPNGAEIPVNRKNRQDFVDAYVDYIFNQSVASLFSAFSAGFHKVCGSKVLELFQPNELQAMVIGNTAYDWKELEKSTEYKGEYWADHPTIKMFWEIFHALPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQPTGGGEQFLPVAHTCFNLLDLPKYSKKETLRSKLIQAIDHYEGFSLV

Target ORF information:

RefSeq Version	XM_031904674.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis HECT and RLD domain containing E3 ubiquitin protein ligase 4 (herc4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031904674.1

ORF Insert Sequence:

ATGTTATGCT GGGGAAATAC ATCTTTTGGA CAGCTGGGAT TAGGGGGGAT TGATGAGGAG 
GTTGTTTTAG AACCGAGAAG ATGTGACTTT TTCATCAATA AAAATGTCCG TGATGTCGGA 
TGTGGAAGCA GACACACAGT TTTTGTCTTG GATGATGGGA CAGTTTACAC CTGCGGATGT 
AATGATCTTG GGCAACTGGG ACATGACAAA AGCAGAAGAA AGCCAGAGCA GGTTGGAGCC 
CTGGATGCTC AGATCATTGT GGCAGTTTCA TGTGGAGAAG CTCACACCTT GGCATTAAAT 
GACAAAGGTC AAGTGTTTTC TTGGGGTCAT GCTGTATATG GACAGATAGG TCTCTCAACT 
GCAGAAGAAT ATATTAGAGT ACCCAGAAAT ATAAAGAGTC TGTCTGATGT ACAGATTGTG 
CAAGTTGCCT GTGGTCATCA TCATTCACTT GCACTGTCCA AAGACAGTGA TATTTTTTCA 
TGGGGACAAA ACCAATATGG CCAGCTTGGC TTGGGTTTAG AAATAAAGAA AGAATGTGCA 
CCCAGACATA TTAAGTCTCT ATCAGGGATT CCCTTCGCTC ATATTGCTGC TGGAGGTGGA 
CACAGTTTTG CGTTGACAAT CTCAGGGGCC ATTTTCGGTT GGGGAAGAAA CAAATTTGGC 
CAGCTTGGTC TTACTGATGA AACTGATCGA AATGTCCCAG CTCTTCTCAA GTCACTTCGC 
TCACAGAAAA TCATTCATAT TTGTTGTGGA GAAGATCATA CAGCTGCTTT AACCAAAGAG 
GGTGGTGTAT TTACATTTGG AGCTGGGGGC TATGGACAGC TTGGTCATAA CTCTACCAAC 
CATGAAGTCA ACCCAAGGAA AGTGTTTGAG CTTATGGGAA GCATTGTGAC ACAAATCGCT 
TGTGGGAGGC AGCACACATT AGCTTTTGTT CCTTCATCAG GACGCATTTA TTCGTTTGGG 
CTTGGTGGCA ATGGGCAGTT AGGAACAGGG TCTACCATTA ACAGAAAAAG TCCTTTCACA 
GTTAAAGGGA ACTGGCTGAC TTATGAAGAC TATGTTCGGC ACCTTCCTGA GGCTAATGGA 
TGTTACTGTT TGAAGAGAAT TTACAGCGGT GGAGACCAGA GTTTTGCACA TTATATTACA 
ATACAGAATT GCCATCCTCC AGATGACTTT AGGAGGTGTA ATTGTTCCAG AAAAATACGG 
ACTTTAGACG AGGGAGTTAT ACAACGATGG CTGAATTACT CCTTGGGAAG ACTTCCATTA 
GAAATAGTAA ATGAAATAGA CAAAGTATTT TCATCAGCTG CATGTTTAAA TGGAAGCTTT 
TTAGTGACAA GCAACAACGA TCACTATATG ACAAGTTCCA AGTTCTCAGG AGTAGACATG 
AATGTTGCTA GACTTCTTCT TCACAAGCTT ATGGATCCAT CACAGCCACA AATAGCCCAA 
CAGGTGGCTG CCAGCTTGGA GAAGAATCTT ATTCCCAGAC TCACAAGTTC TCCCCCCGAC 
ATTGAGTGCT TGCGTTTGTA TCTCATCCTC CCAGAGTGTC CCCCCATGGC TGATGCCAAC 
AACTTAACTT CTTTAGCAAT TCCGTTTGGC ACAGCTATTG TAAACTTGGA AAAAGCACCA 
TTAAAAGTTT TAGAAAACTG GTGGTCCCTA CTTGAGCCTC CGCTTTTCTT GAAAGTAGTA 
CAACTTTATA AGGATGTTGT GGTGCACCTA TTGAAACTCT CCAAAAATGG AATTCCTCTT 
ACGGAGCGAA GATTATTCTA CAGATTTCTT AATACTGCCC TCAGGGTTTT AGAGATCCTG 
CACAGGGTAA ATGAAAAAGC TGCACAGATC ATACCCTATG ATAAATTCTA CATTCATGAA 
GTGCAAGATT TTGTAGATAT CCAAAACGAC TACATTAACT GGATTAAGCA ACAAGCATCT 
GGAATGGATG TGAATCATGG ATTATATGAG GTAGCTGATC CACCAGTTAC AATCTGCACA 
TACCCCTTTG TTTTTGATGC TCAGGCAAAG ACAACACTTC TGCAGACAGA TGCAGTCATT 
CAGATGCAGA TGGCAGTTGA CAATGCTCAT AGGCAAAACA ACTTATCCTG GTTATTTCCT 
GCAACTGAAT CAGTGGATCC TTGTCTGATA TTAGTTGTTC GCAGAGATAA TATTGTTGGT 
GATGCTATGG AAGTCCTAAG GAAAACCAAA AATATAGACT ACAAAAAGCC AATGCGGGTA 
ATCTTTTTTG GAGAAGAAGC AATTGATGCA GGTGGCGTTC GTAAGGAGTT TTTCCTACTC 
ATCATGAGAG AATTACTGGA CCCCAAGTAT GGAATGTTCA GATATTACGA AGAATCCAGA 
TTAATCTGGT TTTCTGATAA GACCTTTGAA GACAGTGATT TATTCCACCT TATTGGTGTA 
GTCTGTGGTT TAGCAATATA CAATTTCACG ATTATTGAGC TTCACTTCCC TTTGGCTTTA 
TATAAGAAGC TTTTAAAAAA GAAACCATCC TTGGAAGACT TAAAGGAGCT GATGCCTATT 
ATCGGACGGA GCATGCAGCA GTTGTTGGAC TATACTGAAG ATGACATTGA AGAGACATTC 
TGCCTCAATT TTACGATCAC AGTAGAAAAC TTCGGAACAA CAGAAGTCAG AGAGCTAATC 
CCAAATGGAG CTGAGATACC AGTGAATAGG AAAAACAGGC AGGACTTCGT AGATGCCTAT 
GTAGACTACA TCTTCAATCA GTCTGTGGCT TCCTTATTCA GTGCGTTTAG TGCAGGATTT 
CACAAAGTGT GTGGTAGCAA GGTACTCGAG CTCTTCCAGC CCAATGAACT GCAGGCCATG 
GTGATTGGCA ATACCGCCTA TGACTGGAAG GAACTCGAAA AAAGCACAGA ATACAAAGGA 
GAGTATTGGG CCGACCACCC TACAATTAAG ATGTTCTGGG AAATTTTTCA TGCATTACCC 
TTGGAAAAGA AAAAACAATT CTTGCTATTC CTTACTGGCA GCGATCGGAT TCCTATTCTC 
GGTATGAAAA GTTTAAAGCT TGTCATTCAG CCAACAGGAG GAGGAGAACA GTTCCTACCA 
GTCGCTCACA CCTGCTTCAA CCTCTTGGAT CTCCCCAAAT ACAGCAAGAA AGAAACTCTG 
CGATCCAAAC TGATTCAAGC TATAGATCAC TATGAAGGCT TTAGCCTCGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

herc4 ( NM_001128650.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001122122.1 Xenopus tropicalis HECT and RLD domain containing E3 ubiquitin protein ligase 4 (herc4), mRNA.

Price & Availability↑

CloneID	OXa21746
Clone ID Related Accession (Same CDS sequence)	NM_001128650.1
Accession Version	NM_001128650.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3150bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	probable E3 ubiquitin-protein ligase HERC4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC167508.1. ##Evidence-Data-START## Transcript exon combination :: BC167508.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028290, SAMD00028291 [ECO:0000348] ##Evidence-Data-END##

ATGTTATGCT GGGGAAATAC ATCTTTTGGA CAGCTGGGAT TAGGGGGGAT TGATGAGGAG 
GTTGTTTTAG AACCGAGAAG ATGTGACTTT TTCATCAATA AAAATGTCCG TGACGTCGGA 
TGTGGAAGCA GACACACAGT TTTTGTCTTG GATGATGGGA CAGTTTACAC CTGCGGATGT 
AATGATCTTG GGCAACTGGG ACATGACAAA AGCAGAAGAA AGCCAGAGCA GGTTGGAGCC 
CTGGATGCTC AGATCATTGT GGCAGTTTCA TGTGGAGAAG CTCACACCTT GGCATTAAAT 
GACAAAGGTC AAGTGTTTTC TTGGGGTCAT GCTGTATATG GACAGATAGG TCTCTCAACT 
GCAGAAGAAT ATATTAGAGT ACCCAGAAAT ATAAAGAGTC TGTCTGATGT ACAGATTGTG 
CAAGTTGCCT GTGGTCATCA TCATTCACTT GCACTGTCCA AAGACAGTGA TATTTTTTCA 
TGGGGACAAA ACCAATATGG CCAGCTTGGC TTGGGTTTAG AAATAAAGAA AGAATGTGCA 
CCCAGACATA TTAAGTCTCT ATCAGGGATT CCCTTCGCTC ATATTGCTGC TGGAGGTGGA 
CACAGTTTTG CGTTGACAAT CTCAGGGGCC ATTTTCGGTT GGGGAAGAAA CAAATTTGGC 
CAGCTTGGTC TTACTGATGA AACTGATCGA AATGTCCCAG CTCTTCTCAA GTCACTTCGC 
TCACAGAAAA TCATTCATAT TTGTTGTGGA GAAGATCATA CAGCTGCTTT AACCAAAGAG 
GGTGGTGTAT TTACATTTGG AGCTGGGGGC TATGGACAGC TTGGTCATAA CTCTACCAAC 
CATGAAGTCA ACCCAAGGAA AGTGTTTGAG CTTATGGGAA GCATTGTGAC ACAAATCGCT 
TGTGGGAGGC AGCACACATT AGCTTTTGTT CCTTCATCAG GACGCATTTA TTCGTTTGGG 
CTTGGTGGCA ATGGGCAGTT AGGAACAGGG TCTACCATTA ACAGAAAAAG TCCTTTCACA 
GTTAAAGGGA ACTGGCTGAC TTATGAAGAC TATGTTCGGC ACCTTCCTGA GGCTAATGGA 
TGTTACTGTT TGAAGAGAAT TTACAGCGGT GGAGACCAGA GTTTTGCACA TTATATTACA 
ATACAGAATT GCCATCCTCC AGATGACTTT AGGAGGTGTA ATTGTTCCAG AAAAATACGG 
ACTTTAGACG AGGGAGTTAT ACAACGATGG CTGAATTACT CCTTGGGAAG ACTTCCATTA 
GAAATAGTAA ATGAAATAGA CAAAGTATTT TCATCAGCTG CATGTTTAAA TGGAAGCTTT 
TTAGTGACAA GCAACAACGA TCACTATATG ACAAGTTCCA AGTTCTCAGG AGTAGACATG 
AATGTTGCTA GACTTCTTCT TCACAAGCTT ATGGATCCAT CACAGCCACA AATAGCCCAA 
CAGGTGGCTG CCAGCTTGGA GAAGAATCTT ATTCCCAGAC TCACAAGTTC TCCCCCCGAC 
ATTGAGTGCT TGCGTTTGTA TCTCATCCTC CCAGAGTGTC CCCCCATGGC TGATGCCAAC 
AACTTAACTT CTTTAGCAAT TCCGTTTGGC ACAGCTATTG TAAACTTGGA AAAAGCACCA 
TTAAAAGTTT TAGAAAACTG GTGGTCCCTA CTTGAGCCTC CGCTTTTCTT GAAAGTAGTA 
CAACTTTATA AGGATGTTGT GGTGCACCTA TTGAAACTCT CCAAAAATGG AATTCCTCTT 
ACGGAGCGAA GATTATTCTA CAGATTTCTT AATACTGCCC TCAGGGTTTT AGAGATCCTG 
CACAGGGTAA ATGAAAAAGC TGCACAGATC ATACCCTATG ATAAATTCTA CATTCATGAA 
GTGCAAGATT TTGTAGATAT CCAAAACGAC TACATTAACT GGATTAAGCA ACAAGCATCT 
GGAATGGTAG CTGATCCACC AGTTACAATC TGCACATACC CCTTTGTTTT TGATGCTCAG 
GCAAAGACAA CACTTCTGCA GACAGATGCA GTCATTCAGA TGCAGATGGC AGTTGACAAT 
GCTCATAGGC AAAACAACTT ATCCTGGTTA TTTCCTGCAA CTGAATCAGT GGATCCTTGT 
CTGATATTAG TTGTTCGCAG AGATAATATT GTTGGTGATG CTATGGAAGT CCTAAGGAAA 
ACCAAAAATA TAGACTACAA AAAGCCAATG CGGGTAATCT TTTTTGGAGA AGAAGCAATT 
GATGCAGGTG GCGTTCGTAA GGAGTTTTTC CTACTCATCA TGAGAGAATT ACTGGACCCC 
AAGTATGGAA TGTTCAGATA TTACGAAGAA TCCAGATTAA TCTGGTTTTC TGATAAGACC 
TTTGAAGACA GTGATTTATT CCACCTTATT GGTGTAGTCT GTGGTTTAGC AATATACAAT 
TTCACGATTA TTGAGCTTCA CTTCCCTTTG GCTTTATATA AGAAGCTTTT AAAAAAGAAA 
CCATCCTTGG AAGACTTAAA GGAGCTGATG CCTATTATCG GACGGAGCAT GCAGCAGTTG 
TTGGACTATA CTGAAGATGA CATTGAAGAG ACATTCTGCC TCAATTTTAC GATCACAGTA 
GAAAACTTCG GAACAACAGA AGTCAGAGAG CTAATCCCAA ATGGAGCTGA GATACCAGTG 
AATAGGAAAA ACAGGCAGGA CTTCGTAGAT GCCTATGTAG ACTACATCTT CAATCAGTCT 
GTGGCTTCCT TATTCAGTGC GTTTAGTGCA GGATTTCACA AAGTGTGTGG TAGCAAGGTA 
CTCGAGCTCT TCCAGCCCAA TGAACTGCAG GCCATGGTGA TTGGCAATAC CGCCTATGAC 
TGGAAGGAAC TCGAAAAAAG CACAGAATAC AAAGGAGAGT ATTGGGCCGA CCACCCTACA 
ATTAAGATGT TCTGGGAAAT TTTTCATGCA TTACCCTTGG AAAAGAAAAA ACAATTCTTG 
CTATTCCTTA CTGGCAGCGA TCGGATTCCT ATTCTCGGTA TGAAAAGTTT AAAGCTTGTC 
ATTCAGCCAA CAGGAGGAGG AGAACAGTTC CTACCAGTCG CTCACACCTG CTTCAACCTC 
TTGGATCTCC CCAAATACAG CAAGAAAGAA ACTCTGCGAT CCAAACTGAT TCAAGCTATA 
GATCACTATG AAGGCTTTAG CCTCGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001122122.1
CDS	181..3330
Translation	MLCWGNTSFGQLGLGGIDEEVVLEPRRCDFFINKNVRDVGCGSRHTVFVLDDGTVYTCGCNDLGQLGHDKSRRKPEQVGALDAQIIVAVSCGEAHTLALNDKGQVFSWGHAVYGQIGLSTAEEYIRVPRNIKSLSDVQIVQVACGHHHSLALSKDSDIFSWGQNQYGQLGLGLEIKKECAPRHIKSLSGIPFAHIAAGGGHSFALTISGAIFGWGRNKFGQLGLTDETDRNVPALLKSLRSQKIIHICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTNHEVNPRKVFELMGSIVTQIACGRQHTLAFVPSSGRIYSFGLGGNGQLGTGSTINRKSPFTVKGNWLTYEDYVRHLPEANGCYCLKRIYSGGDQSFAHYITIQNCHPPDDFRRCNCSRKIRTLDEGVIQRWLNYSLGRLPLEIVNEIDKVFSSAACLNGSFLVTSNNDHYMTSSKFSGVDMNVARLLLHKLMDPSQPQIAQQVAASLEKNLIPRLTSSPPDIECLRLYLILPECPPMADANNLTSLAIPFGTAIVNLEKAPLKVLENWWSLLEPPLFLKVVQLYKDVVVHLLKLSKNGIPLTERRLFYRFLNTALRVLEILHRVNEKAAQIIPYDKFYIHEVQDFVDIQNDYINWIKQQASGMVADPPVTICTYPFVFDAQAKTTLLQTDAVIQMQMAVDNAHRQNNLSWLFPATESVDPCLILVVRRDNIVGDAMEVLRKTKNIDYKKPMRVIFFGEEAIDAGGVRKEFFLLIMRELLDPKYGMFRYYEESRLIWFSDKTFEDSDLFHLIGVVCGLAIYNFTIIELHFPLALYKKLLKKKPSLEDLKELMPIIGRSMQQLLDYTEDDIEETFCLNFTITVENFGTTEVRELIPNGAEIPVNRKNRQDFVDAYVDYIFNQSVASLFSAFSAGFHKVCGSKVLELFQPNELQAMVIGNTAYDWKELEKSTEYKGEYWADHPTIKMFWEIFHALPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQPTGGGEQFLPVAHTCFNLLDLPKYSKKETLRSKLIQAIDHYEGFSLV

Target ORF information:

RefSeq Version	NM_001128650.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis HECT and RLD domain containing E3 ubiquitin protein ligase 4 (herc4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001128650.1

ORF Insert Sequence:

ATGTTATGCT GGGGAAATAC ATCTTTTGGA CAGCTGGGAT TAGGGGGGAT TGATGAGGAG 
GTTGTTTTAG AACCGAGAAG ATGTGACTTT TTCATCAATA AAAATGTCCG TGACGTCGGA 
TGTGGAAGCA GACACACAGT TTTTGTCTTG GATGATGGGA CAGTTTACAC CTGCGGATGT 
AATGATCTTG GGCAACTGGG ACATGACAAA AGCAGAAGAA AGCCAGAGCA GGTTGGAGCC 
CTGGATGCTC AGATCATTGT GGCAGTTTCA TGTGGAGAAG CTCACACCTT GGCATTAAAT 
GACAAAGGTC AAGTGTTTTC TTGGGGTCAT GCTGTATATG GACAGATAGG TCTCTCAACT 
GCAGAAGAAT ATATTAGAGT ACCCAGAAAT ATAAAGAGTC TGTCTGATGT ACAGATTGTG 
CAAGTTGCCT GTGGTCATCA TCATTCACTT GCACTGTCCA AAGACAGTGA TATTTTTTCA 
TGGGGACAAA ACCAATATGG CCAGCTTGGC TTGGGTTTAG AAATAAAGAA AGAATGTGCA 
CCCAGACATA TTAAGTCTCT ATCAGGGATT CCCTTCGCTC ATATTGCTGC TGGAGGTGGA 
CACAGTTTTG CGTTGACAAT CTCAGGGGCC ATTTTCGGTT GGGGAAGAAA CAAATTTGGC 
CAGCTTGGTC TTACTGATGA AACTGATCGA AATGTCCCAG CTCTTCTCAA GTCACTTCGC 
TCACAGAAAA TCATTCATAT TTGTTGTGGA GAAGATCATA CAGCTGCTTT AACCAAAGAG 
GGTGGTGTAT TTACATTTGG AGCTGGGGGC TATGGACAGC TTGGTCATAA CTCTACCAAC 
CATGAAGTCA ACCCAAGGAA AGTGTTTGAG CTTATGGGAA GCATTGTGAC ACAAATCGCT 
TGTGGGAGGC AGCACACATT AGCTTTTGTT CCTTCATCAG GACGCATTTA TTCGTTTGGG 
CTTGGTGGCA ATGGGCAGTT AGGAACAGGG TCTACCATTA ACAGAAAAAG TCCTTTCACA 
GTTAAAGGGA ACTGGCTGAC TTATGAAGAC TATGTTCGGC ACCTTCCTGA GGCTAATGGA 
TGTTACTGTT TGAAGAGAAT TTACAGCGGT GGAGACCAGA GTTTTGCACA TTATATTACA 
ATACAGAATT GCCATCCTCC AGATGACTTT AGGAGGTGTA ATTGTTCCAG AAAAATACGG 
ACTTTAGACG AGGGAGTTAT ACAACGATGG CTGAATTACT CCTTGGGAAG ACTTCCATTA 
GAAATAGTAA ATGAAATAGA CAAAGTATTT TCATCAGCTG CATGTTTAAA TGGAAGCTTT 
TTAGTGACAA GCAACAACGA TCACTATATG ACAAGTTCCA AGTTCTCAGG AGTAGACATG 
AATGTTGCTA GACTTCTTCT TCACAAGCTT ATGGATCCAT CACAGCCACA AATAGCCCAA 
CAGGTGGCTG CCAGCTTGGA GAAGAATCTT ATTCCCAGAC TCACAAGTTC TCCCCCCGAC 
ATTGAGTGCT TGCGTTTGTA TCTCATCCTC CCAGAGTGTC CCCCCATGGC TGATGCCAAC 
AACTTAACTT CTTTAGCAAT TCCGTTTGGC ACAGCTATTG TAAACTTGGA AAAAGCACCA 
TTAAAAGTTT TAGAAAACTG GTGGTCCCTA CTTGAGCCTC CGCTTTTCTT GAAAGTAGTA 
CAACTTTATA AGGATGTTGT GGTGCACCTA TTGAAACTCT CCAAAAATGG AATTCCTCTT 
ACGGAGCGAA GATTATTCTA CAGATTTCTT AATACTGCCC TCAGGGTTTT AGAGATCCTG 
CACAGGGTAA ATGAAAAAGC TGCACAGATC ATACCCTATG ATAAATTCTA CATTCATGAA 
GTGCAAGATT TTGTAGATAT CCAAAACGAC TACATTAACT GGATTAAGCA ACAAGCATCT 
GGAATGGTAG CTGATCCACC AGTTACAATC TGCACATACC CCTTTGTTTT TGATGCTCAG 
GCAAAGACAA CACTTCTGCA GACAGATGCA GTCATTCAGA TGCAGATGGC AGTTGACAAT 
GCTCATAGGC AAAACAACTT ATCCTGGTTA TTTCCTGCAA CTGAATCAGT GGATCCTTGT 
CTGATATTAG TTGTTCGCAG AGATAATATT GTTGGTGATG CTATGGAAGT CCTAAGGAAA 
ACCAAAAATA TAGACTACAA AAAGCCAATG CGGGTAATCT TTTTTGGAGA AGAAGCAATT 
GATGCAGGTG GCGTTCGTAA GGAGTTTTTC CTACTCATCA TGAGAGAATT ACTGGACCCC 
AAGTATGGAA TGTTCAGATA TTACGAAGAA TCCAGATTAA TCTGGTTTTC TGATAAGACC 
TTTGAAGACA GTGATTTATT CCACCTTATT GGTGTAGTCT GTGGTTTAGC AATATACAAT 
TTCACGATTA TTGAGCTTCA CTTCCCTTTG GCTTTATATA AGAAGCTTTT AAAAAAGAAA 
CCATCCTTGG AAGACTTAAA GGAGCTGATG CCTATTATCG GACGGAGCAT GCAGCAGTTG 
TTGGACTATA CTGAAGATGA CATTGAAGAG ACATTCTGCC TCAATTTTAC GATCACAGTA 
GAAAACTTCG GAACAACAGA AGTCAGAGAG CTAATCCCAA ATGGAGCTGA GATACCAGTG 
AATAGGAAAA ACAGGCAGGA CTTCGTAGAT GCCTATGTAG ACTACATCTT CAATCAGTCT 
GTGGCTTCCT TATTCAGTGC GTTTAGTGCA GGATTTCACA AAGTGTGTGG TAGCAAGGTA 
CTCGAGCTCT TCCAGCCCAA TGAACTGCAG GCCATGGTGA TTGGCAATAC CGCCTATGAC 
TGGAAGGAAC TCGAAAAAAG CACAGAATAC AAAGGAGAGT ATTGGGCCGA CCACCCTACA 
ATTAAGATGT TCTGGGAAAT TTTTCATGCA TTACCCTTGG AAAAGAAAAA ACAATTCTTG 
CTATTCCTTA CTGGCAGCGA TCGGATTCCT ATTCTCGGTA TGAAAAGTTT AAAGCTTGTC 
ATTCAGCCAA CAGGAGGAGG AGAACAGTTC CTACCAGTCG CTCACACCTG CTTCAACCTC 
TTGGATCTCC CCAAATACAG CAAGAAAGAA ACTCTGCGAT CCAAACTGAT TCAAGCTATA 
GATCACTATG AAGGCTTTAG CCTCGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

herc4 ( XM_012965815.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821269.1 Xenopus tropicalis HECT and RLD domain containing E3 ubiquitin protein ligase 4 (herc4), transcript variant X1, mRNA.

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CloneID	OXa29858
Clone ID Related Accession (Same CDS sequence)	XM_012965815.1
Accession Version	XM_012965815.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3150bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) and transcript sequence (BC127372.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 31 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTTATGCT GGGGAAATAC ATCTTTTGGA CAGCTGGGAT TAGGGGGGAT TGATGAGGAG 
GTTGTTTTAG AACCGAGAAG ATGTGACTTT TTCATCAATA AAAATGTCCG TGATGTCGGA 
TGTGGAAGCA GACACACAGT TTTTGTCTTG GATGATGGGA CAGTTTACAC CTGCGGATGT 
AATGATCTTG GGCAACTGGG ACATGACAAA AGCAGAAGAA AGCCAGAGCA GGTTGGAGCC 
CTGGATGCTC AGATCATTGT GGCAGTTTCA TGTGGAGAAG CTCACACCTT GGCATTAAAT 
GACAAAGGTC AAGTGTTTTC TTGGGGTCAT GCTGTATATG GACAGATAGG TCTCTCAACT 
GCAGAAGAAT ATATTAGAGT ACCCAGAAAT ATAAAGAGTC TGTCTGATGT ACAGATTGTG 
CAAGTTGCCT GTGGTCATCA TCATTCACTT GCACTGTCCA AAGACAGTGA TATTTTCTCA 
TGGGGACAAA ACCAATATGG CCAGCTTGGC TTGGGTTTAG AAATAAAGAA AGAATGTGCA 
CCCAGACATA TTAAGTCTCT ATCAGGGATT CCCTTCGCTC ATATTGCTGC TGGAGGTGGA 
CACAGTTTTG CGTTGACAAT CTCAGGGGCC ATTTTCGGTT GGGGAAGAAA CAAATTTGGC 
CAGCTTGGTC TTACTGATGA AACTGATCGA AATGTCCCAG CTCTTCTCAA GTCACTTCGC 
TCACAGAAAA TCATTCATAT TTGTTGTGGA GAAGATCATA CAGCTGCTTT AACCAAAGAG 
GGTGGTGTAT TTACATTTGG AGCTGGGGGC TATGGACAGC TTGGTCATAA CTCTACCAAC 
CATGAAGTCA ACCCAAGGAA AGTGTTTGAG CTTATGGGAA GCATTGTGAC ACAAATCGCT 
TGTGGGAGGC AGCACACTTT AGCTTTTGTT CCTTCATCAG GACGCATTTA TTCGTTTGGG 
CTTGGTGGCA ATGGGCAGTT AGGAACAGGG TCTACCATTA ACAGAAAAAG TCCTTTCACA 
GTTAAAGGGA ACTGGCTGAC TTATGAAGAC TATGTTCGGC ACCTTCCTGA GGCTAATGGA 
TGTTACTGTT TGAAGAGAAT TTACAGCGGT GGAGACCAGA GTTTTGCACA TTATATTACA 
ATACAGAATT GCCATCCTCC AGATGACTTT AGGAGGTGTA ATTGTTCCAG AAAAATACGG 
ACTTTAGACG AGGGAGTTAT ACAACGATGG CTGAATTACT CCTTGGGAAG ACTTCCATTA 
GAAATAGTAA ATGAAATAGA CAAAGTATTT TCATCAGCTG CATGTTTAAA TGGAAGCTTT 
TTAGTGACAA GCAACAACGA TCACTATATG ACAAGTTCCA AGTTCTCAGG AGTAGACATG 
AATGTTGCTA GACTTCTTCT TCACAAGCTT ATGGATCCAT CACAGCCACA AATAGCCCAA 
GAGGTGGCTG CCAGCTTGGA GAAGAATCTC ATTCCCAGAC TCACAAGTTC TCCCCCAGAC 
ATTGAGTGCT TGCGTTTGTA TCTCATCCTC CCAGAGTGTC CCCCCATGGC TGATGCCAAC 
AACTTAACTT CTTTAGCAAT TCCGTTTGGC ACAGCTATTG TAAACTTGGA AAAAGCACCA 
TTAAAAGTTT TAGAAAACTG GTGGTCCCTA CTTGAGCCTC CGCTTTTCTT GAAAGTAGTA 
CAACTTTATA AGGATGTTGT GGTGCACCTA TTGAAACTCT CCAAAAATGG AATTCCTCTT 
ACGGAGCGAA GATTATTCTA CAGATTTCTT AATACTGCCC TCAGGGTTTT AGAGATCCTG 
CACAGGGTAA ATGAAAAAGC TGCACAGATC ATACCCTATG ATAAATTCTA CATTCATGAA 
GTGCAAGATT TTGTAGATAT CCAAAACGAC TACATTAACT GGATTAAGCA ACAAGCATCT 
GGAATGGTAG CTGATCCACC AGTTACAATC TGCACATACC CCTTTGTTTT TGATGCTCAG 
GCAAAGACAA CACTTCTGCA GACAGATGCA GTCATTCAGA TGCAGATGGC AGTTGACAAT 
GCTCATAGGC AAAACAACTT ATCCTGGTTA TTTCCTGCAA CTGAATCAGT GGATCCTTGT 
CTGATATTAG TTGTTCGCAG AGATAATATT GTTGGTGATG CTATGGAAGT CCTAAGGAAA 
ACCAAAAATA TAGACTACAA AAAGCCAATG CGGGTAATCT TTTTTGGAGA AGAAGCAATT 
GATGCAGGTG GCGTTCGTAA GGAGTTTTTC CTACTCATCA TGAGAGAATT ACTGGACCCC 
AAGTATGGAA TGTTCAGATA TTACGAAGAA TCCAGATTAA TCTGGTTTTC TGATAAGACC 
TTTGAAGACA GTGATTTATT CCACCTTATT GGTGTAGTCT GTGGTTTAGC AATATACAAT 
TTCACGATTA TTGAGCTTCA CTTCCCTTTG GCTTTATATA AGAAGCTTTT AAAAAAGAAA 
CCATCCTTGG AAGACTTAAA GGAGCTGATG CCTATTATCG GACGGAGCAT GCAGCAGTTG 
TTGGACTATA CTGAAGATGA CATTGAAGAG ACATTCTGCC TCAATTTTAC GATCACAGTA 
GAAAACTTCG GAACAACAGA AGTCAGAGAG CTAATCCCAA ATGGAGCTGA GATACCAGTG 
AATAGGAAAA ACAGGCAGGA CTTCGTAGAT GCCTATGTAG ACTACATCTT CAATCAGTCT 
GTGGCTTCCT TATTCAGTGC GTTTAGTGCA GGATTTCACA AAGTGTGTGG TAGCAAGGTA 
CTCGAGCTCT TCCAGCCCAA TGAACTGCAG GCCATGGTGA TTGGCAATAC CGCCTATGAC 
TGGAAGGAAC TCGAAAAAAG CACAGAATAC AAAGGAGAGT ATTGGGCCGA CCACCCTACA 
ATTAAGATGT TCTGGGAAAT TTTTCATGCA TTACCCTTGG AAAAGAAAAA ACAATTCTTG 
CTATTCCTTA CTGGCAGCGA TCGGATTCCT ATTCTCGGTA TGAAAAGTTT AAAGCTTGTC 
ATTCAGCCAA CAGGAGGAGG AGAACAGTTC CTACCAGTCG CTCACACCTG CTTCAACCTC 
TTGGATCTCC CCAAATACAG CAAGAAAGAA ACTCTGCGAT CCAAACTGAT TCAAGCTATA 
GATCACTATG AAGGCTTTAG CCTCGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821269.1
CDS	254..3403
Translation	MLCWGNTSFGQLGLGGIDEEVVLEPRRCDFFINKNVRDVGCGSRHTVFVLDDGTVYTCGCNDLGQLGHDKSRRKPEQVGALDAQIIVAVSCGEAHTLALNDKGQVFSWGHAVYGQIGLSTAEEYIRVPRNIKSLSDVQIVQVACGHHHSLALSKDSDIFSWGQNQYGQLGLGLEIKKECAPRHIKSLSGIPFAHIAAGGGHSFALTISGAIFGWGRNKFGQLGLTDETDRNVPALLKSLRSQKIIHICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTNHEVNPRKVFELMGSIVTQIACGRQHTLAFVPSSGRIYSFGLGGNGQLGTGSTINRKSPFTVKGNWLTYEDYVRHLPEANGCYCLKRIYSGGDQSFAHYITIQNCHPPDDFRRCNCSRKIRTLDEGVIQRWLNYSLGRLPLEIVNEIDKVFSSAACLNGSFLVTSNNDHYMTSSKFSGVDMNVARLLLHKLMDPSQPQIAQEVAASLEKNLIPRLTSSPPDIECLRLYLILPECPPMADANNLTSLAIPFGTAIVNLEKAPLKVLENWWSLLEPPLFLKVVQLYKDVVVHLLKLSKNGIPLTERRLFYRFLNTALRVLEILHRVNEKAAQIIPYDKFYIHEVQDFVDIQNDYINWIKQQASGMVADPPVTICTYPFVFDAQAKTTLLQTDAVIQMQMAVDNAHRQNNLSWLFPATESVDPCLILVVRRDNIVGDAMEVLRKTKNIDYKKPMRVIFFGEEAIDAGGVRKEFFLLIMRELLDPKYGMFRYYEESRLIWFSDKTFEDSDLFHLIGVVCGLAIYNFTIIELHFPLALYKKLLKKKPSLEDLKELMPIIGRSMQQLLDYTEDDIEETFCLNFTITVENFGTTEVRELIPNGAEIPVNRKNRQDFVDAYVDYIFNQSVASLFSAFSAGFHKVCGSKVLELFQPNELQAMVIGNTAYDWKELEKSTEYKGEYWADHPTIKMFWEIFHALPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQPTGGGEQFLPVAHTCFNLLDLPKYSKKETLRSKLIQAIDHYEGFSLV

Target ORF information:

RefSeq Version	XM_012965815.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis HECT and RLD domain containing E3 ubiquitin protein ligase 4 (herc4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965815.1

ORF Insert Sequence:

ATGTTATGCT GGGGAAATAC ATCTTTTGGA CAGCTGGGAT TAGGGGGGAT TGATGAGGAG 
GTTGTTTTAG AACCGAGAAG ATGTGACTTT TTCATCAATA AAAATGTCCG TGATGTCGGA 
TGTGGAAGCA GACACACAGT TTTTGTCTTG GATGATGGGA CAGTTTACAC CTGCGGATGT 
AATGATCTTG GGCAACTGGG ACATGACAAA AGCAGAAGAA AGCCAGAGCA GGTTGGAGCC 
CTGGATGCTC AGATCATTGT GGCAGTTTCA TGTGGAGAAG CTCACACCTT GGCATTAAAT 
GACAAAGGTC AAGTGTTTTC TTGGGGTCAT GCTGTATATG GACAGATAGG TCTCTCAACT 
GCAGAAGAAT ATATTAGAGT ACCCAGAAAT ATAAAGAGTC TGTCTGATGT ACAGATTGTG 
CAAGTTGCCT GTGGTCATCA TCATTCACTT GCACTGTCCA AAGACAGTGA TATTTTCTCA 
TGGGGACAAA ACCAATATGG CCAGCTTGGC TTGGGTTTAG AAATAAAGAA AGAATGTGCA 
CCCAGACATA TTAAGTCTCT ATCAGGGATT CCCTTCGCTC ATATTGCTGC TGGAGGTGGA 
CACAGTTTTG CGTTGACAAT CTCAGGGGCC ATTTTCGGTT GGGGAAGAAA CAAATTTGGC 
CAGCTTGGTC TTACTGATGA AACTGATCGA AATGTCCCAG CTCTTCTCAA GTCACTTCGC 
TCACAGAAAA TCATTCATAT TTGTTGTGGA GAAGATCATA CAGCTGCTTT AACCAAAGAG 
GGTGGTGTAT TTACATTTGG AGCTGGGGGC TATGGACAGC TTGGTCATAA CTCTACCAAC 
CATGAAGTCA ACCCAAGGAA AGTGTTTGAG CTTATGGGAA GCATTGTGAC ACAAATCGCT 
TGTGGGAGGC AGCACACTTT AGCTTTTGTT CCTTCATCAG GACGCATTTA TTCGTTTGGG 
CTTGGTGGCA ATGGGCAGTT AGGAACAGGG TCTACCATTA ACAGAAAAAG TCCTTTCACA 
GTTAAAGGGA ACTGGCTGAC TTATGAAGAC TATGTTCGGC ACCTTCCTGA GGCTAATGGA 
TGTTACTGTT TGAAGAGAAT TTACAGCGGT GGAGACCAGA GTTTTGCACA TTATATTACA 
ATACAGAATT GCCATCCTCC AGATGACTTT AGGAGGTGTA ATTGTTCCAG AAAAATACGG 
ACTTTAGACG AGGGAGTTAT ACAACGATGG CTGAATTACT CCTTGGGAAG ACTTCCATTA 
GAAATAGTAA ATGAAATAGA CAAAGTATTT TCATCAGCTG CATGTTTAAA TGGAAGCTTT 
TTAGTGACAA GCAACAACGA TCACTATATG ACAAGTTCCA AGTTCTCAGG AGTAGACATG 
AATGTTGCTA GACTTCTTCT TCACAAGCTT ATGGATCCAT CACAGCCACA AATAGCCCAA 
GAGGTGGCTG CCAGCTTGGA GAAGAATCTC ATTCCCAGAC TCACAAGTTC TCCCCCAGAC 
ATTGAGTGCT TGCGTTTGTA TCTCATCCTC CCAGAGTGTC CCCCCATGGC TGATGCCAAC 
AACTTAACTT CTTTAGCAAT TCCGTTTGGC ACAGCTATTG TAAACTTGGA AAAAGCACCA 
TTAAAAGTTT TAGAAAACTG GTGGTCCCTA CTTGAGCCTC CGCTTTTCTT GAAAGTAGTA 
CAACTTTATA AGGATGTTGT GGTGCACCTA TTGAAACTCT CCAAAAATGG AATTCCTCTT 
ACGGAGCGAA GATTATTCTA CAGATTTCTT AATACTGCCC TCAGGGTTTT AGAGATCCTG 
CACAGGGTAA ATGAAAAAGC TGCACAGATC ATACCCTATG ATAAATTCTA CATTCATGAA 
GTGCAAGATT TTGTAGATAT CCAAAACGAC TACATTAACT GGATTAAGCA ACAAGCATCT 
GGAATGGTAG CTGATCCACC AGTTACAATC TGCACATACC CCTTTGTTTT TGATGCTCAG 
GCAAAGACAA CACTTCTGCA GACAGATGCA GTCATTCAGA TGCAGATGGC AGTTGACAAT 
GCTCATAGGC AAAACAACTT ATCCTGGTTA TTTCCTGCAA CTGAATCAGT GGATCCTTGT 
CTGATATTAG TTGTTCGCAG AGATAATATT GTTGGTGATG CTATGGAAGT CCTAAGGAAA 
ACCAAAAATA TAGACTACAA AAAGCCAATG CGGGTAATCT TTTTTGGAGA AGAAGCAATT 
GATGCAGGTG GCGTTCGTAA GGAGTTTTTC CTACTCATCA TGAGAGAATT ACTGGACCCC 
AAGTATGGAA TGTTCAGATA TTACGAAGAA TCCAGATTAA TCTGGTTTTC TGATAAGACC 
TTTGAAGACA GTGATTTATT CCACCTTATT GGTGTAGTCT GTGGTTTAGC AATATACAAT 
TTCACGATTA TTGAGCTTCA CTTCCCTTTG GCTTTATATA AGAAGCTTTT AAAAAAGAAA 
CCATCCTTGG AAGACTTAAA GGAGCTGATG CCTATTATCG GACGGAGCAT GCAGCAGTTG 
TTGGACTATA CTGAAGATGA CATTGAAGAG ACATTCTGCC TCAATTTTAC GATCACAGTA 
GAAAACTTCG GAACAACAGA AGTCAGAGAG CTAATCCCAA ATGGAGCTGA GATACCAGTG 
AATAGGAAAA ACAGGCAGGA CTTCGTAGAT GCCTATGTAG ACTACATCTT CAATCAGTCT 
GTGGCTTCCT TATTCAGTGC GTTTAGTGCA GGATTTCACA AAGTGTGTGG TAGCAAGGTA 
CTCGAGCTCT TCCAGCCCAA TGAACTGCAG GCCATGGTGA TTGGCAATAC CGCCTATGAC 
TGGAAGGAAC TCGAAAAAAG CACAGAATAC AAAGGAGAGT ATTGGGCCGA CCACCCTACA 
ATTAAGATGT TCTGGGAAAT TTTTCATGCA TTACCCTTGG AAAAGAAAAA ACAATTCTTG 
CTATTCCTTA CTGGCAGCGA TCGGATTCCT ATTCTCGGTA TGAAAAGTTT AAAGCTTGTC 
ATTCAGCCAA CAGGAGGAGG AGAACAGTTC CTACCAGTCG CTCACACCTG CTTCAACCTC 
TTGGATCTCC CCAAATACAG CAAGAAAGAA ACTCTGCGAT CCAAACTGAT TCAAGCTATA 
GATCACTATG AAGGCTTTAG CCTCGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Proceedings of the National Academy of Sciences of the United States of America99(26)16899-903(2002 Dec)
Strausberg RL,Feingold EA,Grouse LH,Derge JG,Klausner RD,Collins FS,Wagner L,Shenmen CM,Schuler GD,Altschul SF,Zeeberg B,Buetow KH,Schaefer CF,Bhat NK,Hopkins RF,Jordan H,Moore T,Max SI,Wang J,Hsieh F,Diatchenko L,Marusina K,Farmer AA,Rubin GM,Hong L,Stapleton M,Soares MB,Bonaldo MF,Casavant TL,Scheetz TE,Brownstein MJ,Usdin TB,Toshiyuki S,Carninci P,Prange C,Raha SS,Loquellano NA,Peters GJ,Abramson RD,Mullahy SJ,Bosak SA,McEwan PJ,McKernan KJ,Malek JA,Gunaratne PH,Richards S,Worley KC,Hale S,Garcia AM,Gay LJ,Hulyk SW,Villalon DK,Muzny DM,Sodergren EJ,Lu X,Gibbs RA,Fahey J,Helton E,Ketteman M,Madan A,Rodrigues S,Sanchez A,Whiting M,Madan A,Young AC,Shevchenko Y,Bouffard GG,Blakesley RW,Touchman JW,Green ED,Dickson MC,Rodriguez AC,Grimwood J,Schmutz J,Myers RM,Butterfield YS,Krzywinski MI,Skalska U,Smailus DE,Schnerch A,Schein JE,Jones SJ,Marra MA,

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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herc4 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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