lefty.S cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	lefty.S
Entrez Gene ID	398193
Full Name	left-right determination factor S homeolog
Synonyms	XATV,Xlefty,antivin,atv,leftb,lefty-1,lefty-a,lefty-b,lefty1,leftyb
General protein information	Preferred Names left-right determination factor S homeolog Names left-right determination factor S homeolog TGF-beta family member lefty-B
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001088574.1	NP_001082043.1	left-right determination factor S homeolog precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Rapid differential transport of Nodal and Lefty on sulfated proteoglycan-rich extracellular matrix regulates left-right asymmetry in Xenopus.
Marjoram L, Wright C
Development (Cambridge, England)138(3)475-85(2011 Feb)

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

Expression cloning of Xantivin, a Xenopus lefty/antivin-related gene, involved in the regulation of activin signaling during mesoderm induction.
Tanegashima K, Yokota C, Takahashi S, Asashima M
Mechanisms of development99(1-2)3-14(2000 Dec)

Regulation of gut and heart left-right asymmetry by context-dependent interactions between xenopus lefty and BMP4 signaling.
Branford WW, Essner JJ, Yost HJ
Developmental biology223(2)291-306(2000 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that rapid differential transport of Nodal and Lefty on sulfated proteoglycan-rich extracellular matrix regulates left-right asymmetry in Xenopus.
Title: Rapid differential transport of Nodal and Lefty on sulfated proteoglycan-rich extracellular matrix regulates left-right asymmetry in Xenopus.

The following lefty.S gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lefty.S cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAf04373	NM_001088574.1 Latest version!	Xenopus laevis left-right determination factor S homeolog (lefty.S), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lefty.S ( NM_001088574.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> NP_001082043.1 Xenopus laevis left-right determination factor S homeolog (lefty.S), mRNA.

Price & Availability↑

CloneID	OAf04373
Clone ID Related Accession (Same CDS sequence)	NM_001088574.1
Accession Version	NM_001088574.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-27
Organism	Xenopus laevis(African clawed frog)
Product	left-right determination factor S homeolog precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF283563.1. On Jul 25, 2007 this sequence version replaced NM_001088575.1. ##Evidence-Data-START## Transcript exon combination :: AF283563.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN04277011, SAMN04277012 [ECO:0000348] ##Evidence-Data-END##

ATGGGTGTCA CTACCAAATC TTTGAGACTC TGCTTGATCT TCAGCACCCT GTGCCTGGCT 
GGCATCAGTG CCTTCAACCC AGATGACATC AGAGATGCTC TGCTAAAAAA ACTCAACCTC 
CAAGAGGTCC CCAAACTGGA GAAAAGAGAC GTGGAGAACC TGGTGATCCC CAGGCACATC 
CAAGCCAAGT ACATGTCTAT GCTGCACAAT CACAGAGAGA GGAAGAAGAG ATCACTGCCC 
AGCTTGGCTG GCATCCTCAG GGGCATCTCG GGCAATGCAG ATATTTCCGG GGAGATTCTG 
TACTCAGACT CCAGCAAGCA GTCCCTGGTT TTTGGAATGG AGTCCCGTAT CCCAGAGAAC 
AGTGAGGTTA CCATGGCTGA ACTCAAGCTC TTCAAGAAAC CCCCTAAAAT CATGAACGTT 
CCAGAAAGGA GATTCCACCG ACCTGTCAAC AATGCCAGAG TTAGTGTGTA TTATGTGGAG 
ATATTGAAAG ATGGCACCAA CAGAACCTCC CTGGTGGACT CTAGGCTTGT GCCAATCATG 
GAGTCGGGCT GGAGGAGCTT TGACGTGACC CAGGCTGTGC ATTACTGGAT GAGAAGTGGT 
GGACAGTCAT CAATGCACTT AGAGATCCAC GTGGATGGGG AGCGACATGG AAGTCATGCA 
TCTGAGATGG CAAAGATGGT TCGTTTCACC ACCCAGAGCC CATCAGACAA CTCTCTGGGA 
AAACCAGAGC TTGTCCTGTT TACTCTCAAC CTCAATGAGC AGGGGACCCG TGGAGACTGC 
TCAGCATCAG GTGCCAAGAA AGACAACATC TGCTGCAGAG AGGAATATTT CATTAACTTT 
CGGGAACTTA CCTGGACACA GTACTGGATT ATTGAGCCAG CAGGATACAA CGCTTTTCGG 
TGCACTGGAA GTTGCAAGCA ACCAAAGTAC CCCTTGTCTC ATTATCACTA TGGACAGAGA 
ACATGCGCCG TGGTGGAAAG CGCCCCACTA CCCGTAATGT ACCTGGTCAA AAAGGGAGAC 
TACACGGAAA TCGAAGTGGC GGAGTTCCCT AATATGATTG TCGAAAAATG CGGTTGCACA 
ATGGACAATA TCGCTATCAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001082043.1
CDS	49..1152
Translation	MGVTTKSLRLCLIFSTLCLAGISAFNPDDIRDALLKKLNLQEVPKLEKRDVENLVIPRHIQAKYMSMLHNHRERKKRSLPSLAGILRGISGNADISGEILYSDSSKQSLVFGMESRIPENSEVTMAELKLFKKPPKIMNVPERRFHRPVNNARVSVYYVEILKDGTNRTSLVDSRLVPIMESGWRSFDVTQAVHYWMRSGGQSSMHLEIHVDGERHGSHASEMAKMVRFTTQSPSDNSLGKPELVLFTLNLNEQGTRGDCSASGAKKDNICCREEYFINFRELTWTQYWIIEPAGYNAFRCTGSCKQPKYPLSHYHYGQRTCAVVESAPLPVMYLVKKGDYTEIEVAEFPNMIVEKCGCTMDNIAII

Target ORF information:

RefSeq Version	NM_001088574.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis left-right determination factor S homeolog (lefty.S), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001088574.1

ORF Insert Sequence:

ATGGGTGTCA CTACCAAATC TTTGAGACTC TGCTTGATCT TCAGCACCCT GTGCCTGGCT 
GGCATCAGTG CCTTCAACCC AGATGACATC AGAGATGCTC TGCTAAAAAA ACTCAACCTC 
CAAGAGGTCC CCAAACTGGA GAAAAGAGAC GTGGAGAACC TGGTGATCCC CAGGCACATC 
CAAGCCAAGT ACATGTCTAT GCTGCACAAT CACAGAGAGA GGAAGAAGAG ATCACTGCCC 
AGCTTGGCTG GCATCCTCAG GGGCATCTCG GGCAATGCAG ATATTTCCGG GGAGATTCTG 
TACTCAGACT CCAGCAAGCA GTCCCTGGTT TTTGGAATGG AGTCCCGTAT CCCAGAGAAC 
AGTGAGGTTA CCATGGCTGA ACTCAAGCTC TTCAAGAAAC CCCCTAAAAT CATGAACGTT 
CCAGAAAGGA GATTCCACCG ACCTGTCAAC AATGCCAGAG TTAGTGTGTA TTATGTGGAG 
ATATTGAAAG ATGGCACCAA CAGAACCTCC CTGGTGGACT CTAGGCTTGT GCCAATCATG 
GAGTCGGGCT GGAGGAGCTT TGACGTGACC CAGGCTGTGC ATTACTGGAT GAGAAGTGGT 
GGACAGTCAT CAATGCACTT AGAGATCCAC GTGGATGGGG AGCGACATGG AAGTCATGCA 
TCTGAGATGG CAAAGATGGT TCGTTTCACC ACCCAGAGCC CATCAGACAA CTCTCTGGGA 
AAACCAGAGC TTGTCCTGTT TACTCTCAAC CTCAATGAGC AGGGGACCCG TGGAGACTGC 
TCAGCATCAG GTGCCAAGAA AGACAACATC TGCTGCAGAG AGGAATATTT CATTAACTTT 
CGGGAACTTA CCTGGACACA GTACTGGATT ATTGAGCCAG CAGGATACAA CGCTTTTCGG 
TGCACTGGAA GTTGCAAGCA ACCAAAGTAC CCCTTGTCTC ATTATCACTA TGGACAGAGA 
ACATGCGCCG TGGTGGAAAG CGCCCCACTA CCCGTAATGT ACCTGGTCAA AAAGGGAGAC 
TACACGGAAA TCGAAGTGGC GGAGTTCCCT AATATGATTG TCGAAAAATG CGGTTGCACA 
ATGGACAATA TCGCTATCAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Rapid differential transport of Nodal and Lefty on sulfated proteoglycan-rich extracellular matrix regulates left-right asymmetry in Xenopus.
Development (Cambridge, England)138(3)475-85(2011 Feb)
Marjoram L,Wright C

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

Expression cloning of Xantivin, a Xenopus lefty/antivin-related gene, involved in the regulation of activin signaling during mesoderm induction.
Mechanisms of development99(1-2)3-14(2000 Dec)
Tanegashima K,Yokota C,Takahashi S,Asashima M

Regulation of gut and heart left-right asymmetry by context-dependent interactions between xenopus lefty and BMP4 signaling.
Developmental biology223(2)291-306(2000 Jul)
Branford WW,Essner JJ,Yost HJ