chek2.L cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	chek2.L
Entrez Gene ID	398179
Full Name	checkpoint kinase 2 L homeolog
Synonyms	cds1,chek2,chk2,hucds1,lfs2,pp1425,rad53
General protein information	Preferred Names checkpoint kinase 2 L homeolog Names checkpoint kinase 2 L homeolog CHK2 checkpoint homolog protein kinase Cds1
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001088547.1	NP_001082016.1	checkpoint kinase 2 L homeolog

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Chk2/Cds1 protein kinase blocks apoptosis during early development of Xenopus laevis.
Wroble BN, Sible JC
Developmental dynamics : an official publication of the American Association of Anatomists233(4)1359-65(2005 Aug)

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

Response of Xenopus Cds1 in cell-free extracts to DNA templates with double-stranded ends.
Guo Z, Dunphy WG
Molecular biology of the cell11(5)1535-46(2000 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Chk2/Cds kinase operates at a switch between cell cycle arrest or apoptosis in response to genomic assaults.
Title: Chk2/Cds1 protein kinase blocks apoptosis during early development of Xenopus laevis.

The following chek2.L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the chek2.L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAf10761	NM_001088547.1 Latest version!	Xenopus laevis checkpoint kinase 2 L homeolog (chek2.L), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

chek2.L ( NM_001088547.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> NP_001082016.1 Xenopus laevis checkpoint kinase 2 L homeolog (chek2.L), mRNA.

Price & Availability↑

CloneID	OAf10761
Clone ID Related Accession (Same CDS sequence)	NM_001088547.1
Accession Version	NM_001088547.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-27
Organism	Xenopus laevis(African clawed frog)
Product	checkpoint kinase 2 L homeolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF174295.2. ##Evidence-Data-START## Transcript exon combination :: AF174295.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN04157064, SAMN04158978 [ECO:0000350] ##Evidence-Data-END##

ATGATGTCTC GTGATACTAA AACAGAGTCG CAACAATCTC AGGGCACTTC AAGTTCCTCG 
TCCTCCAGTG CTCCTCAATC TTACAGTCAG TCGTCTTCAT CGGGTACATT AAGTTCTTTG 
GATACTGTTC CAGTGCAAGA TCTTGCGTCC ATTCCTGAAG ACCCTGAGAT AGACGAGGAT 
ATACCCCAGC CTTGGGGTCG TCTCTGGGCT CTTGGGAAGG GCTTTCTAAA TCATGATTGC 
CTGCATGAAG AATATGTATT TGGAAGAGAC AAAAAATGTG ATTACACCTT TGATATTCCA 
GTACTAAACC AGACCGACAG GTACAAAACA TATAGCAAAA GGCACTTCAG AATATTTCAG 
GAATTAGGTC ATGGACACTC CCGTGTTGCT AACATAGAAG ATCTGAGCGG CAACGGAACA 
TTTGTTAACA AGGAGATTAT TGGAAAAGGG CGGACATTGC CTTTAACAAA TAATGCCGAG 
ATTGCACTTT CATTACCAAC TAATAAAGTT TTTGTTTTTT CAGATTTGTC TGTGGATGAT 
CAGACTATAT ATCCTAAGGA CTTCATTGAT AAATACATCA TGTCAAGGCC AATCGGAAGT 
GGGGCTTGCG GGGAAGTGAA ATTGGCTTTT CAAAAGTCAG TATGCAAGAA GGTTGCTGTA 
AAAATCATCA GTAAAAGAAA ATTTAAAATG AACACTTCTA GTAATGAACA CCCTATATCT 
GTTGACACAG AAATAGAGAT CCTGAAAAAA CTTGATCATC CCTGTATCAT TAAAATAGAG 
AATTTTTTTG ACTCTGAGGA CTTCTATTAC ATTGTGTTGG AACTGATGGA AGGAGGCGAA 
CTGTTTGACA GGGTGGTAAA TTCGACAAGA CTCCGAGAAC CAATTGCCAA ACTGTATTTT 
TATCAGATGC TGCTAGCTGT TCAGTACCTC CATGAAAATG GGGTGATACA TCGTGATCTG 
AAGCCTGAAA ATGTGCTGTT GTCATCCACT AGTGAAGAAT GTTGCATAAA GATAACGGAT 
TTTGGACAGT CAAAAATTCT GGGTGAAACG TCTTTAATGA GAACTTTGTG TGGAACTCCT 
ACATACTTGG CGCCTGAAGT TTTGAATACA GCAGGCACAA CTGGATACAG TAGTGCAGTG 
GATTGCTGGA GTTTAGGAGT CATCCTTTTT GTGTGTCTTT GTGGATATCC CCCCTTTTCA 
GAACAAAATA GTAACATTCC CTTGAAAAAT CAGATTGCAG AGGGAAAATA CACCTACATT 
GCTGCTGCTT GGAGAAATGT ATCAGAACAA GCATTTGATT TAGTCAAGAA TCTTCTTGTT 
GTTGATCCTG AGCAAAGACT TACCACTAAA CAAGCACTGG AACATCCCTG GCTTCAGGAC 
GATTCTATGA AGCATACTGT TGAAAGGTTA ATGTATGGGG TTGACCACAC AATGCCTCCT 
CCAATCAAGA AAAACATAAT TCGAAAACGG GGACATGAAT GGGATCAAGA TGCCAGTACT 
TCATCTTGCT CAGAGATATT ACCAACATCA GCCGAAAAGA GAGCAAAAAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001082016.1
CDS	224..1777
Translation	MMSRDTKTESQQSQGTSSSSSSSAPQSYSQSSSSGTLSSLDTVPVQDLASIPEDPEIDEDIPQPWGRLWALGKGFLNHDCLHEEYVFGRDKKCDYTFDIPVLNQTDRYKTYSKRHFRIFQELGHGHSRVANIEDLSGNGTFVNKEIIGKGRTLPLTNNAEIALSLPTNKVFVFSDLSVDDQTIYPKDFIDKYIMSRPIGSGACGEVKLAFQKSVCKKVAVKIISKRKFKMNTSSNEHPISVDTEIEILKKLDHPCIIKIENFFDSEDFYYIVLELMEGGELFDRVVNSTRLREPIAKLYFYQMLLAVQYLHENGVIHRDLKPENVLLSSTSEECCIKITDFGQSKILGETSLMRTLCGTPTYLAPEVLNTAGTTGYSSAVDCWSLGVILFVCLCGYPPFSEQNSNIPLKNQIAEGKYTYIAAAWRNVSEQAFDLVKNLLVVDPEQRLTTKQALEHPWLQDDSMKHTVERLMYGVDHTMPPPIKKNIIRKRGHEWDQDASTSSCSEILPTSAEKRAKR

Target ORF information:

RefSeq Version	NM_001088547.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis checkpoint kinase 2 L homeolog (chek2.L), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001088547.1

ORF Insert Sequence:

ATGATGTCTC GTGATACTAA AACAGAGTCG CAACAATCTC AGGGCACTTC AAGTTCCTCG 
TCCTCCAGTG CTCCTCAATC TTACAGTCAG TCGTCTTCAT CGGGTACATT AAGTTCTTTG 
GATACTGTTC CAGTGCAAGA TCTTGCGTCC ATTCCTGAAG ACCCTGAGAT AGACGAGGAT 
ATACCCCAGC CTTGGGGTCG TCTCTGGGCT CTTGGGAAGG GCTTTCTAAA TCATGATTGC 
CTGCATGAAG AATATGTATT TGGAAGAGAC AAAAAATGTG ATTACACCTT TGATATTCCA 
GTACTAAACC AGACCGACAG GTACAAAACA TATAGCAAAA GGCACTTCAG AATATTTCAG 
GAATTAGGTC ATGGACACTC CCGTGTTGCT AACATAGAAG ATCTGAGCGG CAACGGAACA 
TTTGTTAACA AGGAGATTAT TGGAAAAGGG CGGACATTGC CTTTAACAAA TAATGCCGAG 
ATTGCACTTT CATTACCAAC TAATAAAGTT TTTGTTTTTT CAGATTTGTC TGTGGATGAT 
CAGACTATAT ATCCTAAGGA CTTCATTGAT AAATACATCA TGTCAAGGCC AATCGGAAGT 
GGGGCTTGCG GGGAAGTGAA ATTGGCTTTT CAAAAGTCAG TATGCAAGAA GGTTGCTGTA 
AAAATCATCA GTAAAAGAAA ATTTAAAATG AACACTTCTA GTAATGAACA CCCTATATCT 
GTTGACACAG AAATAGAGAT CCTGAAAAAA CTTGATCATC CCTGTATCAT TAAAATAGAG 
AATTTTTTTG ACTCTGAGGA CTTCTATTAC ATTGTGTTGG AACTGATGGA AGGAGGCGAA 
CTGTTTGACA GGGTGGTAAA TTCGACAAGA CTCCGAGAAC CAATTGCCAA ACTGTATTTT 
TATCAGATGC TGCTAGCTGT TCAGTACCTC CATGAAAATG GGGTGATACA TCGTGATCTG 
AAGCCTGAAA ATGTGCTGTT GTCATCCACT AGTGAAGAAT GTTGCATAAA GATAACGGAT 
TTTGGACAGT CAAAAATTCT GGGTGAAACG TCTTTAATGA GAACTTTGTG TGGAACTCCT 
ACATACTTGG CGCCTGAAGT TTTGAATACA GCAGGCACAA CTGGATACAG TAGTGCAGTG 
GATTGCTGGA GTTTAGGAGT CATCCTTTTT GTGTGTCTTT GTGGATATCC CCCCTTTTCA 
GAACAAAATA GTAACATTCC CTTGAAAAAT CAGATTGCAG AGGGAAAATA CACCTACATT 
GCTGCTGCTT GGAGAAATGT ATCAGAACAA GCATTTGATT TAGTCAAGAA TCTTCTTGTT 
GTTGATCCTG AGCAAAGACT TACCACTAAA CAAGCACTGG AACATCCCTG GCTTCAGGAC 
GATTCTATGA AGCATACTGT TGAAAGGTTA ATGTATGGGG TTGACCACAC AATGCCTCCT 
CCAATCAAGA AAAACATAAT TCGAAAACGG GGACATGAAT GGGATCAAGA TGCCAGTACT 
TCATCTTGCT CAGAGATATT ACCAACATCA GCCGAAAAGA GAGCAAAAAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chk2/Cds1 protein kinase blocks apoptosis during early development of Xenopus laevis.
Developmental dynamics : an official publication of the American Association of Anatomists233(4)1359-65(2005 Aug)
Wroble BN,Sible JC

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

Response of Xenopus Cds1 in cell-free extracts to DNA templates with double-stranded ends.
Molecular biology of the cell11(5)1535-46(2000 May)
Guo Z,Dunphy WG