ccnb1.2.S cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ccnb1.2.S
Entrez Gene ID	397742
Full Name	cyclin B1 S homeolog
Synonyms	ccnb,ccnb1,ccnb1.2
General protein information	Preferred Names cyclin B1 S homeolog Names G2/mitotic-specific cyclin-B1 C-B1
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001087797.1	NP_001081266.1	G2/mitotic-specific cyclin-B1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

A novel p34(cdc2)-binding and activating protein that is necessary and sufficient to trigger G(2)/M progression in Xenopus oocytes.
Ferby I, Blazquez M, Palmer A, Eritja R, Nebreda AR
Genes & development13(16)2177-89(1999 Aug)

Translation of cyclin mRNA is necessary for extracts of activated xenopus eggs to enter mitosis.
Minshull J, Blow JJ, Hunt T
Cell56(6)947-56(1989 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ccnb1.2.S gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ccnb1.2.S cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAf00795	NM_001087797.1 Latest version!	Xenopus laevis cyclin B1 S homeolog (ccnb1.2.S), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ccnb1.2.S ( NM_001087797.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> NP_001081266.1 Xenopus laevis cyclin B1 S homeolog (ccnb1.2.S), mRNA.

Price & Availability↑

CloneID	OAf00795
Clone ID Related Accession (Same CDS sequence)	NM_001087797.1
Accession Version	NM_001087797.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1194bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Xenopus laevis(African clawed frog)
Product	G2/mitotic-specific cyclin-B1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from J03166.1. On Jul 25, 2007 this sequence version replaced NM_001087798.1. ##Evidence-Data-START## Transcript exon combination :: J03166.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00012418, SAMD00012419 [ECO:0000348] ##Evidence-Data-END##

ATGTCGCTAC GAGTCACCAG AAACATGCTG GCAAATGCAG AAAACAATGT GAAAACCACT 
TTGGCTGGAA AGAGGGTTGT TGCTACCAAA CCAGGGTTGA GACCTCGTAC AGCCTTGGGA 
GACATTGGAA ACAAGGCAGA GGTGAAAGTG CCAACAAAAA AGGAATTAAA GCCAGCAGTA 
AAAGCTGCCA AGAAGGCAAA ACCTGTTGAC AAATTGTTGG AGCCTCTTAA AGTGATAGAA 
GAGAATGTTT GCCCTAAACC TGCTCAGGTT GAACCCAGCT CACCAAGCCC AATGGAAACA 
TCTGGTTGCC TCCCTGATGA GCTCTGCCAG GCTTTCTCTG ATGTCCTCAT TCACGTTAAA 
GATGTTGATG CTGATGATGA TGGCAACCCA ATGCTGTGCA GTGAATATGT CAAGGACATT 
TATGCTTACC TGAGGAGCCT TGAGGATGCA CAAGCAGTCA GACAAAACTA CCTTCATGGA 
CAGGAAGTCA CAGGCAACAT GCGTGCCATT TTGATTGACT GGCTGGTCCA GGTGCAAATG 
AAATTCCGTC TACTGCAGGA GACAATGTTC ATGACTGTTG GCATAATTGA CCGCTTTCTG 
CAGGAACATC CAGTTCCCAA AAACCAGCTA CAGCTTGTGG GGGTCACGGC TATGTTCCTT 
GCTGCTAAAT ATGAAGAGAT GTACCCACCA GAAATTGGAG ACTTTACATT TGTAACTGAT 
CACACATACA CAAAGGCTCA AATTCGGGAC ATGGAAATGA AGATACTTAG GGTGCTAAAG 
TTTGCAATTG GCCGACCCTT ACCCCTGCAC TTTCTTCGGA GAGCTTCTAA AATTGGAGAG 
GTAACTGCTG AACAGCATAG TTTAGCCAAA TATTTGATGG AACTTGTGAT GGTGGATTAT 
GATATGGTAC ATTTCACGCC TTCCCAAATA GCAGCTGCTT CCTCCTGCTT GTCTCTCAAA 
ATCTTAAATG CAGGTGACTG GACCCCAACA CTCCATCACT ATATGGCTTA CTCTGAAGAA 
GATCTAGTCC CTGTTATGCA GCATATGGCC AAGAACATCA TCAAGGTGAA CAAAGGACTA 
ACCAAGCATC TGACTGTTAA GAACAAGTAT GCTAGCAGCA AACAAATGAA GATCAGCACG 
ATTCCACAGC TGAGGTCAGA TGTTGTTGTG GAAATGGCCC GCCCACTCAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001081266.1
CDS	94..1287
Translation	MSLRVTRNMLANAENNVKTTLAGKRVVATKPGLRPRTALGDIGNKAEVKVPTKKELKPAVKAAKKAKPVDKLLEPLKVIEENVCPKPAQVEPSSPSPMETSGCLPDELCQAFSDVLIHVKDVDADDDGNPMLCSEYVKDIYAYLRSLEDAQAVRQNYLHGQEVTGNMRAILIDWLVQVQMKFRLLQETMFMTVGIIDRFLQEHPVPKNQLQLVGVTAMFLAAKYEEMYPPEIGDFTFVTDHTYTKAQIRDMEMKILRVLKFAIGRPLPLHFLRRASKIGEVTAEQHSLAKYLMELVMVDYDMVHFTPSQIAAASSCLSLKILNAGDWTPTLHHYMAYSEEDLVPVMQHMAKNIIKVNKGLTKHLTVKNKYASSKQMKISTIPQLRSDVVVEMARPLM

Target ORF information:

RefSeq Version	NM_001087797.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis cyclin B1 S homeolog (ccnb1.2.S), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001087797.1

ORF Insert Sequence:

ATGTCGCTAC GAGTCACCAG AAACATGCTG GCAAATGCAG AAAACAATGT GAAAACCACT 
TTGGCTGGAA AGAGGGTTGT TGCTACCAAA CCAGGGTTGA GACCTCGTAC AGCCTTGGGA 
GACATTGGAA ACAAGGCAGA GGTGAAAGTG CCAACAAAAA AGGAATTAAA GCCAGCAGTA 
AAAGCTGCCA AGAAGGCAAA ACCTGTTGAC AAATTGTTGG AGCCTCTTAA AGTGATAGAA 
GAGAATGTTT GCCCTAAACC TGCTCAGGTT GAACCCAGCT CACCAAGCCC AATGGAAACA 
TCTGGTTGCC TCCCTGATGA GCTCTGCCAG GCTTTCTCTG ATGTCCTCAT TCACGTTAAA 
GATGTTGATG CTGATGATGA TGGCAACCCA ATGCTGTGCA GTGAATATGT CAAGGACATT 
TATGCTTACC TGAGGAGCCT TGAGGATGCA CAAGCAGTCA GACAAAACTA CCTTCATGGA 
CAGGAAGTCA CAGGCAACAT GCGTGCCATT TTGATTGACT GGCTGGTCCA GGTGCAAATG 
AAATTCCGTC TACTGCAGGA GACAATGTTC ATGACTGTTG GCATAATTGA CCGCTTTCTG 
CAGGAACATC CAGTTCCCAA AAACCAGCTA CAGCTTGTGG GGGTCACGGC TATGTTCCTT 
GCTGCTAAAT ATGAAGAGAT GTACCCACCA GAAATTGGAG ACTTTACATT TGTAACTGAT 
CACACATACA CAAAGGCTCA AATTCGGGAC ATGGAAATGA AGATACTTAG GGTGCTAAAG 
TTTGCAATTG GCCGACCCTT ACCCCTGCAC TTTCTTCGGA GAGCTTCTAA AATTGGAGAG 
GTAACTGCTG AACAGCATAG TTTAGCCAAA TATTTGATGG AACTTGTGAT GGTGGATTAT 
GATATGGTAC ATTTCACGCC TTCCCAAATA GCAGCTGCTT CCTCCTGCTT GTCTCTCAAA 
ATCTTAAATG CAGGTGACTG GACCCCAACA CTCCATCACT ATATGGCTTA CTCTGAAGAA 
GATCTAGTCC CTGTTATGCA GCATATGGCC AAGAACATCA TCAAGGTGAA CAAAGGACTA 
ACCAAGCATC TGACTGTTAA GAACAAGTAT GCTAGCAGCA AACAAATGAA GATCAGCACG 
ATTCCACAGC TGAGGTCAGA TGTTGTTGTG GAAATGGCCC GCCCACTCAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

A novel p34(cdc2)-binding and activating protein that is necessary and sufficient to trigger G(2)/M progression in Xenopus oocytes.
Genes & development13(16)2177-89(1999 Aug)
Ferby I,Blazquez M,Palmer A,Eritja R,Nebreda AR

Translation of cyclin mRNA is necessary for extracts of activated xenopus eggs to enter mitosis.
Cell56(6)947-56(1989 Mar)
Minshull J,Blow JJ,Hunt T